LOCUS       DAECNO010000001        89421 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.001, whole genome shotgun sequence.
ACCESSION   DAECNO010000001 DAECNO010000000
VERSION     DAECNO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.001"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(86..469)
                     /gene="rplQ"
                     /locus_tag="KKO08_000001"
     CDS             complement(86..469)
                     /gene="rplQ"
                     /locus_tag="KKO08_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBD6316422.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(510..1499)
                     /gene="rpoA"
                     /locus_tag="KKO08_000002"
     CDS             complement(510..1499)
                     /gene="rpoA"
                     /locus_tag="KKO08_000002"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBD6316423.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(1525..2145)
                     /gene="rpsD"
                     /locus_tag="KKO08_000003"
     CDS             complement(1525..2145)
                     /gene="rpsD"
                     /locus_tag="KKO08_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBD6316424.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(2179..2568)
                     /gene="rpsK"
                     /locus_tag="KKO08_000004"
     CDS             complement(2179..2568)
                     /gene="rpsK"
                     /locus_tag="KKO08_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBD6316425.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2585..2941)
                     /gene="rpsM"
                     /locus_tag="KKO08_000005"
     CDS             complement(2585..2941)
                     /gene="rpsM"
                     /locus_tag="KKO08_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBD6316426.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3088..3204)
                     /gene="rpmJ"
                     /locus_tag="KKO08_000006"
     CDS             complement(3088..3204)
                     /gene="rpmJ"
                     /locus_tag="KKO08_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBD6316427.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(3236..4567)
                     /gene="secY"
                     /locus_tag="KKO08_000007"
     CDS             complement(3236..4567)
                     /gene="secY"
                     /locus_tag="KKO08_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBD6316428.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(4575..5009)
                     /gene="rplO"
                     /locus_tag="KKO08_000008"
     CDS             complement(4575..5009)
                     /gene="rplO"
                     /locus_tag="KKO08_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBD6316429.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(5013..5192)
                     /gene="rpmD"
                     /locus_tag="KKO08_000009"
     CDS             complement(5013..5192)
                     /gene="rpmD"
                     /locus_tag="KKO08_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBD6316430.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(5196..5699)
                     /gene="rpsE"
                     /locus_tag="KKO08_000010"
     CDS             complement(5196..5699)
                     /gene="rpsE"
                     /locus_tag="KKO08_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBD6316431.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(5714..6067)
                     /gene="rplR"
                     /locus_tag="KKO08_000011"
     CDS             complement(5714..6067)
                     /gene="rplR"
                     /locus_tag="KKO08_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBD6316432.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(6077..6610)
                     /gene="rplF"
                     /locus_tag="KKO08_000012"
     CDS             complement(6077..6610)
                     /gene="rplF"
                     /locus_tag="KKO08_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBD6316433.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(6623..7015)
                     /gene="rpsH"
                     /locus_tag="KKO08_000013"
     CDS             complement(6623..7015)
                     /gene="rpsH"
                     /locus_tag="KKO08_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBD6316434.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(7049..7354)
                     /gene="rpsN"
                     /locus_tag="KKO08_000014"
     CDS             complement(7049..7354)
                     /gene="rpsN"
                     /locus_tag="KKO08_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBD6316435.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7369..7908)
                     /gene="rplE"
                     /locus_tag="KKO08_000015"
     CDS             complement(7369..7908)
                     /gene="rplE"
                     /locus_tag="KKO08_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBD6316436.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(7923..8237)
                     /gene="rplX"
                     /locus_tag="KKO08_000016"
     CDS             complement(7923..8237)
                     /gene="rplX"
                     /locus_tag="KKO08_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBD6316437.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(8248..8619)
                     /gene="rplN"
                     /locus_tag="KKO08_000017"
     CDS             complement(8248..8619)
                     /gene="rplN"
                     /locus_tag="KKO08_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBD6316438.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(8784..9038)
                     /gene="rpsQ"
                     /locus_tag="KKO08_000018"
     CDS             complement(8784..9038)
                     /gene="rpsQ"
                     /locus_tag="KKO08_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBD6316439.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(9038..9229)
                     /gene="rpmC"
                     /locus_tag="KKO08_000019"
     CDS             complement(9038..9229)
                     /gene="rpmC"
                     /locus_tag="KKO08_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBD6316440.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(9229..9639)
                     /gene="rplP"
                     /locus_tag="KKO08_000020"
     CDS             complement(9229..9639)
                     /gene="rplP"
                     /locus_tag="KKO08_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBD6316441.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(9652..10353)
                     /gene="rpsC"
                     /locus_tag="KKO08_000021"
     CDS             complement(9652..10353)
                     /gene="rpsC"
                     /locus_tag="KKO08_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBD6316442.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(10371..10703)
                     /gene="rplV"
                     /locus_tag="KKO08_000022"
     CDS             complement(10371..10703)
                     /gene="rplV"
                     /locus_tag="KKO08_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBD6316443.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(10718..10996)
                     /gene="rpsS"
                     /locus_tag="KKO08_000023"
     CDS             complement(10718..10996)
                     /gene="rpsS"
                     /locus_tag="KKO08_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBD6316444.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(11013..11834)
                     /gene="rplB"
                     /locus_tag="KKO08_000024"
     CDS             complement(11013..11834)
                     /gene="rplB"
                     /locus_tag="KKO08_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBD6316445.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(11852..12154)
                     /gene="rplW"
                     /locus_tag="KKO08_000025"
     CDS             complement(11852..12154)
                     /gene="rplW"
                     /locus_tag="KKO08_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBD6316446.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(12151..12756)
                     /gene="rplD"
                     /locus_tag="KKO08_000026"
     CDS             complement(12151..12756)
                     /gene="rplD"
                     /locus_tag="KKO08_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBD6316447.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(12767..13396)
                     /gene="rplC"
                     /locus_tag="KKO08_000027"
     CDS             complement(12767..13396)
                     /gene="rplC"
                     /locus_tag="KKO08_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBD6316448.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(13429..13740)
                     /gene="rpsJ"
                     /locus_tag="KKO08_000028"
     CDS             complement(13429..13740)
                     /gene="rpsJ"
                     /locus_tag="KKO08_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBD6316449.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            14121..14588
                     /locus_tag="KKO08_000029"
     CDS             14121..14588
                     /locus_tag="KKO08_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD6316450.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            complement(14585..15061)
                     /gene="bfr"
                     /locus_tag="KKO08_000030"
     CDS             complement(14585..15061)
                     /gene="bfr"
                     /locus_tag="KKO08_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBD6316451.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(15134..15328)
                     /gene="bfd"
                     /locus_tag="KKO08_000031"
     CDS             complement(15134..15328)
                     /gene="bfd"
                     /locus_tag="KKO08_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBD6316452.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            complement(15511..16695)
                     /gene="tuf"
                     /locus_tag="KKO08_000032"
     CDS             complement(15511..16695)
                     /gene="tuf"
                     /locus_tag="KKO08_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD6316453.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LG"
     gene            complement(16766..18880)
                     /gene="fusA"
                     /locus_tag="KKO08_000033"
     CDS             complement(16766..18880)
                     /gene="fusA"
                     /locus_tag="KKO08_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBD6316454.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(18977..19447)
                     /gene="rpsG"
                     /locus_tag="KKO08_000034"
     CDS             complement(18977..19447)
                     /gene="rpsG"
                     /locus_tag="KKO08_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBD6316455.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(19544..19918)
                     /gene="rpsL"
                     /locus_tag="KKO08_000035"
     CDS             complement(19544..19918)
                     /gene="rpsL"
                     /locus_tag="KKO08_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBD6316456.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(20044..20331)
                     /gene="tusB"
                     /locus_tag="KKO08_000036"
     CDS             complement(20044..20331)
                     /gene="tusB"
                     /locus_tag="KKO08_000036"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBD6316457.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(20339..20698)
                     /gene="tusC"
                     /locus_tag="KKO08_000037"
     CDS             complement(20339..20698)
                     /gene="tusC"
                     /locus_tag="KKO08_000037"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBD6316458.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(20698..21084)
                     /gene="tusD"
                     /locus_tag="KKO08_000038"
     CDS             complement(20698..21084)
                     /gene="tusD"
                     /locus_tag="KKO08_000038"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBD6316459.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(21084..21806)
                     /locus_tag="KKO08_000039"
     CDS             complement(21084..21806)
                     /locus_tag="KKO08_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6316460.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(21973..22785)
                     /gene="fkpA"
                     /locus_tag="KKO08_000040"
     CDS             complement(21973..22785)
                     /gene="fkpA"
                     /locus_tag="KKO08_000040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD6316461.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            23006..23224
                     /locus_tag="KKO08_000041"
     CDS             23006..23224
                     /locus_tag="KKO08_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBD6316462.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(23273..23863)
                     /gene="slyD"
                     /locus_tag="KKO08_000042"
     CDS             complement(23273..23863)
                     /gene="slyD"
                     /locus_tag="KKO08_000042"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD6316463.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(23958..24158)
                     /locus_tag="KKO08_000043"
     CDS             complement(23958..24158)
                     /locus_tag="KKO08_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBD6316464.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(24168..25905)
                     /gene="kefB"
                     /locus_tag="KKO08_000044"
                     /pseudo
     CDS             complement(24168..25905)
                     /gene="kefB"
                     /locus_tag="KKO08_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(25905..26456)
                     /gene="kefG"
                     /locus_tag="KKO08_000045"
     CDS             complement(25905..26456)
                     /gene="kefG"
                     /locus_tag="KKO08_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBD6316465.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            26587..28500
                     /locus_tag="KKO08_000046"
     CDS             26587..28500
                     /locus_tag="KKO08_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6316466.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            28500..29522
                     /locus_tag="KKO08_000047"
     CDS             28500..29522
                     /locus_tag="KKO08_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD6316467.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            29516..29734
                     /locus_tag="KKO08_000048"
     CDS             29516..29734
                     /locus_tag="KKO08_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBD6316468.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            29788..30657
                     /locus_tag="KKO08_000049"
     CDS             29788..30657
                     /locus_tag="KKO08_000049"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBD6316469.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(30712..31116)
                     /locus_tag="KKO08_000050"
     CDS             complement(30712..31116)
                     /locus_tag="KKO08_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBD6316470.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            31418..32050
                     /gene="crp"
                     /locus_tag="KKO08_000051"
     CDS             31418..32050
                     /gene="crp"
                     /locus_tag="KKO08_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBD6316471.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            32101..34191
                     /locus_tag="KKO08_000052"
     CDS             32101..34191
                     /locus_tag="KKO08_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBD6316472.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(34258..35478)
                     /gene="argD/dapC"
                     /locus_tag="KKO08_000053"
     CDS             complement(34258..35478)
                     /gene="argD/dapC"
                     /locus_tag="KKO08_000053"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBD6316473.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(35564..36127)
                     /gene="pabA"
                     /locus_tag="KKO08_000054"
     CDS             complement(35564..36127)
                     /gene="pabA"
                     /locus_tag="KKO08_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBD6316474.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(36159..36761)
                     /locus_tag="KKO08_000055"
     CDS             complement(36159..36761)
                     /locus_tag="KKO08_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBD6316475.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(36751..36918)
                     /locus_tag="KKO08_000056"
     CDS             complement(36751..36918)
                     /locus_tag="KKO08_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBD6316476.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(37023..37604)
                     /gene="ppiA"
                     /locus_tag="KKO08_000057"
                     /gene_synonym="rotA"
     CDS             complement(37023..37604)
                     /gene="ppiA"
                     /locus_tag="KKO08_000057"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBD6316477.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            37875..39056
                     /gene="tsgA"
                     /locus_tag="KKO08_000058"
     CDS             37875..39056
                     /gene="tsgA"
                     /locus_tag="KKO08_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBD6316478.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            39318..41861
                     /gene="nirB"
                     /locus_tag="KKO08_000059"
     CDS             39318..41861
                     /gene="nirB"
                     /locus_tag="KKO08_000059"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBD6316479.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            41858..42184
                     /gene="nirD"
                     /locus_tag="KKO08_000060"
     CDS             41858..42184
                     /gene="nirD"
                     /locus_tag="KKO08_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBD6316480.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            42310..43116
                     /gene="nirC"
                     /locus_tag="KKO08_000061"
     CDS             42310..43116
                     /gene="nirC"
                     /locus_tag="KKO08_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBD6316481.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            43135..44508
                     /gene="cobA"
                     /locus_tag="KKO08_000062"
     CDS             43135..44508
                     /gene="cobA"
                     /locus_tag="KKO08_000062"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD6316482.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            44755..44922
                     /locus_tag="KKO08_000063"
     CDS             44755..44922
                     /locus_tag="KKO08_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBD6316483.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            45217..46554
                     /gene="frlA"
                     /locus_tag="KKO08_000064"
                     /pseudo
     CDS             45217..46554
                     /gene="frlA"
                     /locus_tag="KKO08_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            46575..47597
                     /gene="frlB"
                     /locus_tag="KKO08_000065"
     CDS             46575..47597
                     /gene="frlB"
                     /locus_tag="KKO08_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBD6316484.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            47647..48477
                     /gene="frlC"
                     /locus_tag="KKO08_000066"
     CDS             47647..48477
                     /gene="frlC"
                     /locus_tag="KKO08_000066"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBD6316485.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            48474..49259
                     /gene="frlD"
                     /locus_tag="KKO08_000067"
     CDS             48474..49259
                     /gene="frlD"
                     /locus_tag="KKO08_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBD6316486.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            49359..50090
                     /gene="phoB"
                     /locus_tag="KKO08_000068"
     CDS             49359..50090
                     /gene="phoB"
                     /locus_tag="KKO08_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBD6316487.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(50250..51335)
                     /locus_tag="KKO08_000069"
     CDS             complement(50250..51335)
                     /locus_tag="KKO08_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBD6316488.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(51347..52651)
                     /locus_tag="KKO08_000070"
     CDS             complement(51347..52651)
                     /locus_tag="KKO08_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBD6316489.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(52663..53016)
                     /locus_tag="KKO08_000071"
     CDS             complement(52663..53016)
                     /locus_tag="KKO08_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBD6316490.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(53027..53905)
                     /locus_tag="KKO08_000072"
     CDS             complement(53027..53905)
                     /locus_tag="KKO08_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBD6316491.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(53902..55128)
                     /locus_tag="KKO08_000073"
     CDS             complement(53902..55128)
                     /locus_tag="KKO08_000073"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD6316492.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(55129..56292)
                     /locus_tag="KKO08_000074"
     CDS             complement(55129..56292)
                     /locus_tag="KKO08_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBD6316493.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(56376..56738)
                     /locus_tag="KKO08_000075"
     CDS             complement(56376..56738)
                     /locus_tag="KKO08_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBD6316494.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(56755..57660)
                     /locus_tag="KKO08_000076"
     CDS             complement(56755..57660)
                     /locus_tag="KKO08_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316495.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(57930..58934)
                     /gene="trpS"
                     /locus_tag="KKO08_000077"
     CDS             complement(57930..58934)
                     /gene="trpS"
                     /locus_tag="KKO08_000077"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBD6316496.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(58927..59685)
                     /gene="gph"
                     /locus_tag="KKO08_000078"
     CDS             complement(58927..59685)
                     /gene="gph"
                     /locus_tag="KKO08_000078"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBD6316497.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(59678..60355)
                     /gene="rpe"
                     /locus_tag="KKO08_000079"
     CDS             complement(59678..60355)
                     /gene="rpe"
                     /locus_tag="KKO08_000079"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBD6316498.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(60373..61209)
                     /gene="dam"
                     /locus_tag="KKO08_000080"
     CDS             complement(60373..61209)
                     /gene="dam"
                     /locus_tag="KKO08_000080"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD6316499.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(61316..62602)
                     /gene="damX"
                     /locus_tag="KKO08_000081"
     CDS             complement(61316..62602)
                     /gene="damX"
                     /locus_tag="KKO08_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBD6316500.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(62694..63782)
                     /gene="aroB"
                     /locus_tag="KKO08_000082"
     CDS             complement(62694..63782)
                     /gene="aroB"
                     /locus_tag="KKO08_000082"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBD6316501.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(63839..64360)
                     /gene="aroK"
                     /locus_tag="KKO08_000083"
     CDS             complement(63839..64360)
                     /gene="aroK"
                     /locus_tag="KKO08_000083"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBD6316502.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(64761..65999)
                     /gene="hofQ"
                     /locus_tag="KKO08_000084"
     CDS             complement(64761..65999)
                     /gene="hofQ"
                     /locus_tag="KKO08_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBD6316503.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(65911..66315)
                     /gene="hofP"
                     /locus_tag="KKO08_000085"
     CDS             complement(65911..66315)
                     /gene="hofP"
                     /locus_tag="KKO08_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBD6316504.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(66305..66745)
                     /gene="hofO"
                     /locus_tag="KKO08_000086"
     CDS             complement(66305..66745)
                     /gene="hofO"
                     /locus_tag="KKO08_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBD6316505.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(66729..67268)
                     /gene="hofN"
                     /locus_tag="KKO08_000087"
     CDS             complement(66729..67268)
                     /gene="hofN"
                     /locus_tag="KKO08_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBD6316506.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(67268..68047)
                     /gene="hofM"
                     /locus_tag="KKO08_000088"
     CDS             complement(67268..68047)
                     /gene="hofM"
                     /locus_tag="KKO08_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBD6316507.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            68167..70719
                     /gene="mrcA"
                     /locus_tag="KKO08_000089"
     CDS             68167..70719
                     /gene="mrcA"
                     /locus_tag="KKO08_000089"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBD6316508.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(70884..71444)
                     /gene="nudE"
                     /locus_tag="KKO08_000090"
     CDS             complement(70884..71444)
                     /gene="nudE"
                     /locus_tag="KKO08_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBD6316509.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            71764..73899
                     /gene="igaA"
                     /locus_tag="KKO08_000091"
     CDS             71764..73899
                     /gene="igaA"
                     /locus_tag="KKO08_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBD6316510.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            73964..74632
                     /gene="yrfG"
                     /locus_tag="KKO08_000092"
     CDS             73964..74632
                     /gene="yrfG"
                     /locus_tag="KKO08_000092"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBD6316511.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            74643..75044
                     /gene="hslR"
                     /locus_tag="KKO08_000093"
     CDS             74643..75044
                     /gene="hslR"
                     /locus_tag="KKO08_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBD6316512.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            75069..75938
                     /gene="hslO"
                     /locus_tag="KKO08_000094"
     CDS             75069..75938
                     /gene="hslO"
                     /locus_tag="KKO08_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBD6316513.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(76082..77806)
                     /locus_tag="KKO08_000095"
     CDS             complement(76082..77806)
                     /locus_tag="KKO08_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBD6316514.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            78185..79807
                     /gene="pckA"
                     /locus_tag="KKO08_000096"
     CDS             78185..79807
                     /gene="pckA"
                     /locus_tag="KKO08_000096"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBD6316515.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(79883..81235)
                     /gene="envZ"
                     /locus_tag="KKO08_000097"
     CDS             complement(79883..81235)
                     /gene="envZ"
                     /locus_tag="KKO08_000097"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBD6316516.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(81232..81951)
                     /gene="ompR"
                     /locus_tag="KKO08_000098"
     CDS             complement(81232..81951)
                     /gene="ompR"
                     /locus_tag="KKO08_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBD6316517.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            82179..82655
                     /gene="greB"
                     /locus_tag="KKO08_000099"
     CDS             82179..82655
                     /gene="greB"
                     /locus_tag="KKO08_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBD6316518.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            82752..85073
                     /locus_tag="KKO08_000100"
     CDS             82752..85073
                     /locus_tag="KKO08_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBD6316519.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSETGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            85511..85738
                     /gene="feoA"
                     /locus_tag="KKO08_000101"
     CDS             85511..85738
                     /gene="feoA"
                     /locus_tag="KKO08_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBD6316520.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            85755..88076
                     /gene="feoB"
                     /locus_tag="KKO08_000102"
     CDS             85755..88076
                     /gene="feoB"
                     /locus_tag="KKO08_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBD6316521.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            88076..88312
                     /gene="feoC"
                     /locus_tag="KKO08_000103"
     CDS             88076..88312
                     /gene="feoC"
                     /locus_tag="KKO08_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBD6316522.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            88515..89402
                     /gene="rpnA"
                     /locus_tag="KKO08_000104"
     CDS             88515..89402
                     /gene="rpnA"
                     /locus_tag="KKO08_000104"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBD6316523.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        21541 a        24170 c        22340 g        21370 t
ORIGIN      
        1 aaaagagtac agctattgta gataagtggg gatactacgg gtataaaaaa acccgccggg
       61 gcgggttttt ttacattgct tcagattact ctgcagcagc ttctgctttc tctgaacgat
      121 caaccagctc gatgtaagcc atcggcgcgt tgtcgcctgc acggaagcca cacttcagaa
      181 tacgagtgta accaccggca cggctcgcga aacgcgggcc cagttcgtta aacagttttg
      241 ccacgatctc gttatcacga gtacgggcga atgccagacg acgattagca acgctatcag
      301 tcttggcaag agtaatcagc ggctcaacta cgcggcgcag ctctttcgct ttaggcagag
      361 tcgtcttgat gatttcatga cgaaccagtg aacctgccat attgcggaac atagcctggc
      421 gatggctgct gttgcggttc agttgacgac cactcttacg atggcgcatg accttatcct
      481 tctcagtaaa accttaacct gtgatccggt tactcgtcag cgatgcttgc cggtggccag
      541 ttttccaagc gcatgcccag agacagtcca cgggaagcca gcacgtcttt aatctcagta
      601 agagattttt taccaaggtt aggcgtttta aggagctcaa cctcggtacg ctgtaccaga
      661 tcaccgatat agtggatagc ttctgcttta aggcagttag cagagcggac agtcaattcc
      721 agatcgtcaa cagggcgcag caggatcgga tcgaactctg gtttctcttc tttcacttca
      781 ggctgacgta catcacgtaa gtcaacgaaa gcttccagtt gttcagccag aatggttgcc
      841 gcacgacgaa tcgcctcttc aggatcgatt gtgccgttgg tttccatttc gatgaccagc
      901 ttgtccaggt cggtacgctg ttctacacgc gctgcttcaa cattgtaggc aatacgctcc
      961 acagggctgt agcatgcgtc gaccagcaga cggccgattg ggcgctcatc ttcttccgaa
     1021 tgaattcggg tagaagccgg cacataacca cgaccgcgct gaactttgat acgcatgcta
     1081 atagacgcgt tctcatcggt caggtggcag atcacgtgct gcggcttgac gatttcgaca
     1141 tcaccgtcgt gggtgatatc ggctgcagtc acagggccaa tgccagattt attcaaggta
     1201 agaataactt catctttgcc ctgaactctc accgccagcc ctttcaggtt gagcaggatt
     1261 tccaggatat cttcctgaac gccttctttg gtgctgtact catgtagtac accatcaatc
     1321 tcaacctcgg tcaccgcgca acccggcatc gatgagagca gaatacggcg cagtgcgtta
     1381 cccagagtat ggccaaagcc acgctctaaa ggctcaaggg tcaccttggc gtgcgtcgaa
     1441 ctcacttgct cgatatcaac caggcgcggt tttagaaact ctgtcacaga accctgcatt
     1501 gtgtcctctc tttggtacta agctttactt ggagtaaagc tcgacgatca ggtgttcgtt
     1561 aatgtccgca gacagatcag aacgctccgg cttacgctta aacgtacctt ccatcttgcc
     1621 agcatcaact tccagccaag ttggcttttc acgctgctca gccagctcca gagcggcttt
     1681 cacgcgagac tgcttcttcg ctttctcacg aatgcttaca acgtcattcg gactaacctg
     1741 ataagaagcg atgttaacaa cacgaccgtt taccataatt gctttatggc taaccagctg
     1801 acgtgcttct gcacgagtgg caccgaagcc catacggtat acaacgttgt ccagacgacc
     1861 ttccagcaga gccaacaggt tttcaccggt gttgcctttc agacgtgctg cttctttgta
     1921 gtagttacgg aactgacgct ccagcacacc atagatacgg cgaacttttt gcttttcacg
     1981 caactgcaca ccatagtcag acagacgcgg tttacgcgca ccgtgctggc caggagcttg
     2041 ttcaatttta cacttggtat cgatcgcgcg aacgccagac ttaaggaata agtcggtgcc
     2101 ctcacgacgg ctcagcttga gcttaggacc caaatatctt gccattttct ttctccaaca
     2161 aacctggaaa acgaggcgtt atacgcgacg ttttttcggc ggacgacaac cgttatgagg
     2221 gatcggagtc acatcagtaa tgttagtgat gcggaaacct gcggcgttca gagcacgaat
     2281 agtagattcg cggcctggac ccggaccttt aaccataact tccagattct tgatgccgta
     2341 ttctttcacg gcgtcagcgc aacgctctgc tgcaacctga gctgcaaacg gagtggattt
     2401 gcgagaacca cggaaaccgg aaccaccggc tgttgcccaa cccaacgcgt taccctgacg
     2461 atcagtgata gtcacgatgg tgttgttgaa agaagcatgg atatgagcca cgccgtcaga
     2521 gacttgtttt cttacacgtt tacgtgcacg aattggtgcc tttgccatta ttcaatcacc
     2581 ccgattattt cttgatcggt ttgcgcggac ccttacgggt acgtgcgttg gtcttggtac
     2641 gctgaccgcg aaccgggaga ccacgacgat gacgcaaacc gcgatagcaa ccaagatcca
     2701 tcaggcgctt gatgctcatg ctgatttcac ggcgcagatc accttcaacg acaaatttgg
     2761 caacttcgtc acgcagcgtg tcgatttgtc cttcagacag ctcactgatc ttaacatctt
     2821 cagcgatacc cgctgcagcc aggatggctt tagaacgggt cttgccgacg ccataaatcg
     2881 aagttaatgc gattacggca tgcttatgat caggaatgtt aatgcctgct atacgggcca
     2941 ctatgcactc ctactattta atatgtacgt tccatgctga aaagcccgtt ttcaggatac
     3001 tcaaatggaa acgtacagac atacaaaaga ttggctggct aatctagcca gctcaaccca
     3061 actttgcaag aaaaatatgc gaaaaaatca gccttggcgc tgtttatgct tcggctcggc
     3121 actgcaaatc acacggatga caccatcacg cttaacgatt ttgcagttac ggcataattt
     3181 cttgacggaa gcacgaactt tcatttttac tctccgtaac ttctcgggcg accaattatc
     3241 ggccgtagcc tttcaggttc gccttcttca atgcagactc atactgactg gacatcatca
     3301 gagtttgcac ttgagccata aagtccataa tcacgacaac aacgataagc agtgaggtcc
     3361 caccgaagta gaacggtact ttcattgcat cacgcatgaa ctccgggatc aggcagataa
     3421 aggtaatata cagcgcacca accaaggtca ggcgggtcat tactttatcg atatacttcg
     3481 ccgtttgctc tcccggacga attcctggta caaatgcacc ggacttcttc aggttatctg
     3541 ctgtttcacg cgggttgaaa accaacgccg tgtagaagaa acagaagaag atgattgcag
     3601 acgcatagag taacacataa agcggttgcc caggctgcaa atacagcgaa attgttgtca
     3661 gccagttcca accagtaccg cccccgaacc atgacgcgat ggtcgccggg aacagaataa
     3721 tactggaagc gaagattgcc gggattaccc ccgccatatt cactttcagc ggtaaatgtg
     3781 tgctctgtgc agcatagaca cgacgacctt gctgacgttt cgcgtagttt accacaatgc
     3841 ggcgttgacc acgctcaaca aacacaacaa agaacgtcac tgcaaatact aatactgcaa
     3901 ccaacagcaa cacgaggaag tgcaggtcgc cttgacgcgc ttgctcgata gtatgggcaa
     3961 tggctggcgg gagtcccgcg acaataccgg cgaagataat gattgaaata ccgttgccga
     4021 tacctcgttc agtgatctgt tcgcccaacc acatcaggaa catcgttcct gtgaccagac
     4081 ttacaacagc ggtgaagtag aatgcaaagc ccgggttaat caccaggcct tgcataccag
     4141 gcatattcgg cagaccggta gcaataccga tcgactggaa tattgccagc accagagtac
     4201 cgtaacgggt gtactggctg atcttacgac gaccagactc cccttctttc ttaatttctg
     4261 ccaacgttgg gtgaaccacc gtcagcagct ggataatgat cgacgccgaa atatacggca
     4321 tgatccccag agcaaagata gaagcacggc tgagagcacc accagagaac atgttaaaca
     4381 tctcaatgat ggtgcctcgc tgttgctcaa gcagtttggc aagtacagcg gcatcaatac
     4441 cagggatcgg aataaaagag ccaatacgga acacaatcag cgcaccgata acaaacagca
     4501 gtctgcgttt cagctcgcct aagccacctt tggcactttg aaaatctaat cccggttgtt
     4561 tagccatctg ctacttattc ctcgatttta ccgccagcag cttcgatagc agcacgagcg
     4621 cctttagtaa cacgcaggcc acgaacagtt accggagtcg ttacttcgcc agccaggatc
     4681 actttcgcga actcgatctg gataccgata atgttagccg ctttcagcgt gttcaggtct
     4741 acaacaccgc cttctacttt agccaggtca gatagacgaa cttcggctgt aatcgctgct
     4801 ttacgagaag tgaagccgaa tttcggcaga cgacggtaca gaggcatctg accaccctcg
     4861 aaaccgcgac gtacgccacc gccagaacga gacttctgac ctttgtgacc acgaccaccg
     4921 gttttaccga ggccagaacc gataccacga cccaggcgtt tacccgcctt tttggagcct
     4981 tcggccggag acagagtatt taaacgcatc tcttactcct caactttaac catgaaggaa
     5041 accgcgttga tcataccgcg aatagcagga gtatcctcgc gctctacggt gtgaccaata
     5101 cgacgcagac ccaagccaag cagcgttgcc ttgtgtttcg gcagacgacc gattgcactg
     5161 cgggtttgag taattttaat agtctttgcc atggtttatt tccccagaat ttcttcaacg
     5221 gatttaccac gcttggcagc gaccatttct ggagaattca tattttccag gccatcaata
     5281 gttgcacgaa ccacgttgat cgggttggtg gaaccatatg ctttagccag aacgttatga
     5341 accccagcga cttccagaac ggcgcgcatt gcaccaccgg cgatgatacc ggtaccttcg
     5401 gaagccggct gcatgaatac gcgagaaccc gtgtgaacac ctttaacagg gtgttgtaga
     5461 gtgccgttat tcagcgcgac gttaatcata ttgcgacggg ctttttccat cgctttctgg
     5521 atcgctgctg gaacttcacg cgctttaccg taaccaaaac caacgcgacc gttaccatcg
     5581 ccaactacag tcagagctgt gaaggagaaa atacgaccac ctttaacggt tttagatacg
     5641 cggtttaccg cgatcagctt ttcctgcagt tcgccagctt gtttttcgat gtgagccatc
     5701 ttacacctct accttagaac tgaaggccag cttcacgggc agcatctgcc agtgcctgga
     5761 cacgaccatg atattggaac ccggaacggt caaaggatac atctttgatg cctttttcca
     5821 gagcgcgttc agcgacagct ttacccacag ctgcagccgc gtctttgtta ccggtgtact
     5881 tcagttgttc agcgatagct ttttctacag tagaagcagc taccagaact tcagaaccgt
     5941 tcggtgcaat tacctgtgcg taaatgtgac gcggggtacg atgtaccacc aggcgagttg
     6001 cgcccagctc ctggagcttg cggcgtgcgc gggtcgcacg acggatacga gcagatttct
     6061 tatccatagt gttaccttac ttcttcttag cctctttggt acgcacgact tcgtcggcgt
     6121 aacgaacacc cttgcctttg taaggctcag gacgacggta ggcgcgcaga tccgctgcaa
     6181 cctggccgat cacctgctta tcagcgcctt tcagcacgat ttcagtctga gtcggacatt
     6241 cagcagtgat acccgcaggc agctgatggt caacaggatg agagaaaccc agagacaggt
     6301 taatcacatt gcctttaacc gctgcacggt aacctacacc aaccagctgc agcttcttag
     6361 tgaagccttc ggtaacaccg ataaccattg agttcagcag ggcacgcgcg gtaccagcct
     6421 gtgcccaacc gtctgcgtaa ccatcacgcg gaccgaaggt cagggtatta tctgcatgtt
     6481 taacttcaac agcatcgttg agagtacgag tcagctcgcc gtttttacct ttgatcgtaa
     6541 taacctgacc gttgattttt acgtcaacgc cggcaggaac aacgaccggt gctttagcaa
     6601 cacgagacat tttttcctcc gattaggcta cgtagcagat aatttcgcca ccaagaccag
     6661 cctggcgcgc tgcacgatca gtcataacac ctttagaggt agaaacaact gcgataccca
     6721 gacccgccat aactttcggc agctcatctt tacgtttata gatgcgcaga cctgggcggc
     6781 tgacacgctg aatgctttct acaacagctt tgccctggaa atacttcaga gtaagttcca
     6841 gttcaggctt ggtgtcgcct tcaactttaa aatcttcaat aaaaccttct tccttcagca
     6901 cgttggcgat tgccactttc agcttggagg aaggcatggt gaccgcagct ttgttcgcgg
     6961 cctgaccgtt acggatacgg gtcagcatat ccgcgatcgg atcttgcatg ctcatctgtc
     7021 tttactcccg tgattcaatt ggtgacaatt accagctagc ctttttcaga cccgggattt
     7081 caccgcgcat agcggcttca cggaccttaa tacggctcaa cccgaacttc cgcaggaaac
     7141 catgcggacg acctgtttga cggcagcggt tacgctgacg agacgggctg gaatcacgcg
     7201 gcagagtctg cagcttgaga acagcgttcc aacgatcttc gtcggaagcg ttcacatcag
     7261 agatgatcgc tttcagttca gcgcgtttcg cgaagtattt atcagctaaa gctacgcgtt
     7321 ttacttcgcg tgctttcatt gattgcttag ccatttagta accctacctt acttgcggaa
     7381 cgggaagtca aaggcagcca gcagagcgcg gccttcttcg tcagatttcg cagtagtggt
     7441 aatggtaata tccaaaccac gaacgcggtc gactttatcg tagtcgattt ctgggaagat
     7501 gatctgctca cggacaccca tgctgtagtt accacgaccg tcgaaagact tagcggacag
     7561 gccacggaag tcacggatac gaggtacagc aatagtgatc aggcgctcaa agaactccca
     7621 catgcgttcg ccacgcagag ttactttaca gccgatcgga tagccctgac ggattttgaa
     7681 gcctgcaaca gatttgcgtg ctttggtgat cagcggtttt tgaccggaga ttgctgccag
     7741 gtctgctgct gcgttatcca gcagtttttt gtcagcgatc gcttcaccaa cacccatgtt
     7801 cagggtgatc ttctcgaccc gagggacttg catgacagaa ttgtagttaa actcagtcat
     7861 gagtttttta actacttcgt ctttgtagta atcatgcagt ttcgccatcg tactactcca
     7921 aattacttga tagtttcgct gttagacttg aagaaacgga cttttttgcc gtcttcgaat
     7981 ctaaagccta cacggtcagc cttgccggtt gccgcattga agattgctac gttggaaacc
     8041 tgaatagcgg cttctttttc aacgatgcca cccggttggt tcagggccgg aaccggcttc
     8101 tgatgtttct taaccaggtt gataccttca acaatgacct tgccggaaga caggacattc
     8161 ttaactttac cgcgtttacc tttatcttta ccggttaaca cgataacttc gtcatcacga
     8221 cggattttcg ctgccatgat tcgctcctta gagtacttct ggtgccagag agataatttt
     8281 catgaacttc tcactacgaa gctcacgagt taccggccca aaaatacgcg taccgatagg
     8341 ctgctcgctg ttgttgttca gaagaacaca agcattacca tcgaagcgaa tgacagaacc
     8401 gtccgggcga cgaacaccct tcttggtgcg caccactacc gccttcagca catcaccttt
     8461 tttgacctta ccacgcggaa ttgcttcttt gatggtgatc ttgatgatgt cgcctacgcc
     8521 tgcgtagcga cggtgcgagc cacccagaac cttgatacac attacgcgac gtgcaccgga
     8581 gttgtcggcg acgttcagca tagtctgttc ttggatcatt ttagtgctcc gctaatgtca
     8641 actactactg agacccgaaa atcaggtcgt taaaaatccc catatcgagg gcgcggcatt
     8701 ataacaccgc ttcaaggata tgggtagaaa aaataaacgg ctcatttctg agccgtttat
     8761 tcgtatcgag agagtgtact gtattacaga accgctttct ctacaacgcg aaccagcgtc
     8821 caggatttag tcttggacag cggacggcat tcgcggattt caaccacgtc accgataccg
     8881 cattcgttgt tctcgtcatg tacgtgcagt ttggtcgtac gcttgatgaa tttaccgtag
     8941 atcgggtgtt tcacaaaacg ttcgatagca acaacaatgg atttctccat tttgtcgcta
     9001 acaacgcgac cttgcagagt acggatttta tcggtcatta cgcacccgcc ttctcgttca
     9061 gtaaagtctt aacgcgtgcg acatcgcgac gcacttgctt caacaggtga gactgttgca
     9121 gctggccact tgcagcctgc atacgcaggt tgaactgctc acgcagcagg ttcagcagct
     9181 cggtgttcag ctcttcaatg ctcttctcac gcagctcttt tgctttcatt acatcaccgt
     9241 cttagttaca aaggtggttt taatcggcag tttcgctgct gccagcttga atgcttcacg
     9301 ggccagctct tccggaacac cgtccatttc atacaggact ttacccggct gaatcaaggc
     9361 aacccaatac tccacgttac ctttaccttt acccatacgc actgccagcg gcttttcagt
     9421 gatcggtttg tccgggaaca cacggatcca gatcttacct tgacgcttaa ctgcacgggt
     9481 catagcacga cgtgctgctt cgatctgacg ggcagtcaga cgaccacggc caacagcttt
     9541 cagaccgaag ctgccgaagc taacatccgt accctgcgcc agaccgcggt tacggccttt
     9601 gtgcatttta cggaattttg tacgctttgg ttgtaacatc agcgacgctc cttatttacg
     9661 gcctttacgc tgctgctttt taggctgagc agccggtttt tccggttgtt caacagcagc
     9721 cataccaccc aggatctcgc ctttgaagat ccacacttta acgccgatta caccgtaagt
     9781 ggtgtgcgct tcagaggtgt tgtagtcgat gtcagcacgc agagtgtgca gcggtacgcg
     9841 accttcgcgg taccattcgg tacgtgcgat ttccgcgccg cccagacggc cgctaacttc
     9901 aactttaata cctttagcgc ccagacgcat tgcgttctgt acagcacgct tcatagcacg
     9961 acggaacata acgcgacgtt ccagctgaga agtgatgctg tcagcaacca gttttgcgtc
    10021 cagttcaggc ttacgaactt cggcgatgtt gatctgtgca ggaacgccag cgatgtccgc
    10081 tacgacctta cgcagttttt ctacgtcttc acctttttta ccgataacga tacccgggcg
    10141 agcagtgtga atggttacac ggatgctctt agccggacgc tcgataacga tacgagatac
    10201 ggacgcttta gccagttcct tagtcaggta ctgacgtact ttaaaatcgc tgtccaggtt
    10261 gtcagcgaat tctttggtgt tcgcaaacca agtagagttc catggtttta caatacccaa
    10321 gcgaatacca ttaggatgta ctttctgacc cattgctagt ctccagagtc tcagcgatcg
    10381 gacacaacca cagtgatgtg gctggtgcgc ttcaggatgc gatctgcacg accttttgca
    10441 cgcggcataa tgcgcttcat gctcgggcct tcgtctacga aaattttcgt aactttcaga
    10501 tcgtcaatgt cagcgccatc gttgtgttca gcgttagcaa tggcagattc cagaactttc
    10561 ttgacctgta cagccgcttt cttgttggtg taggtcaaaa tatccagagc ctgcgacact
    10621 ttcttaccgc gaatcaggtc agcaacaagg cgaaccttct gagcagaaga acgagcatgg
    10681 cgatgtttag caatagtttc catctcttcc tcctacctta tttcttcttc gcttttttat
    10741 cagcagcgtg gccgcgataa gtacgagtcg gtgcgaattc acccagtttg tgaccgacca
    10801 tttcgtcggt tacaaatacc ggaacgtgct gacgaccatt atggacagcg atggtcaaac
    10861 cgatcatgtt aggaaagatc gttgaacgac gggaccaagt gcgcaggggc ttcttgtctc
    10921 cgctttccac cgctttctct accttcttca gcaagtgcag gtcaataaaa ggacctttct
    10981 tgagagaacg tggcatggct tatcctctaa aattatttgc tacggcgacg tacgatgaat
    11041 ttatcagtac gcttgttgct gcgggtcttc ttacctttgg tctgaacgcc ccacggagtt
    11101 accgggtgct taccaaagtt acgaccttca ccaccaccat gtgggtggtc taccgggttc
    11161 atcgcggtac cgcgaacggt cggacgaaca ccacgccagc gtgcagcacc tgctttaccc
    11221 agaacgcgca gcatatgctc agcattgcca acttcgccca gagttgcacg gcagtctgct
    11281 tctactttac gcatttcacc agaacgcaga cgcagggtga cataagcacc atcacgagca
    11341 acgatctgaa cgtaagtacc agcggaacgt gccagctgac cgcctttacc tggtttcatt
    11401 tctacgttat gaacagtaga accaaccggg atgttgcgca tcggcagggt gttacctggt
    11461 ttgattgcag catcaacgcc agactgaatc tggtcgccag ctttcaggcc tttaggggcc
    11521 aggatgtaac ggcgttcacc gtctttgtac agaaccagcg cgatgttcgc ggaacggttc
    11581 ggatcgtact caagacgttc aacaactgcc gggataccgt ctttgttgcg tttgaagtca
    11641 acaatacggt aagcctgctt gtggccacca ccgatatgac gagtggtgat acggccattg
    11701 ttgttacgac caccggattt gctgtttttt tccagcaacg gagcaaaagg tttgcccttg
    11761 tgcagctcag ggttaaccac tttaactacg tggcgacgac ccggagatgt cggtttacat
    11821 ttaacaactg ccattgtatt actcctccga cttactcagc gccgccaacg aagtccagat
    11881 tctggccttc tttcagggtg acgtaagctt ttttccagtc gctacgacga ccgatacgct
    11941 gtccgtgacg tttaactttc cctttaacta ccagggtgtt aacgacttcg acttcgactt
    12001 caaacagttt ctgcacagca gctttgattt ctgctttggt cgcgtctttc gcaactttga
    12061 gtacgatggt gttggatttt tccatcgcag tagacgcttt ttcagaaacg tgcggtgcac
    12121 gcagcacctt cagcagacgt tcttcacgaa tcatgccagc atctcctcaa cttgcttaac
    12181 agcatcagca gtcattacga ctttgtcgaa ggcgatcagg ctaaccgggt cgataccagt
    12241 tgcatcgcgt acgtcaacct tctgcaggtt gcgcgcagcc aggaacaggt tttcgtccag
    12301 ctcaccggtg atgatcagca catcttccag agccatgtct ttcagtttct gtgccagcag
    12361 cttagttttc ggcgcttcta cagagaactt ctcgacaacg atcagacgat cctgacgtac
    12421 cagttcggac aggatgcttt tcagcgcgcc gcggtacatc ttcttgttaa ctttttgact
    12481 gtggtcctgc ggacgagcag caaaggtcac gccaccagaa cgccagatcg ggctcttgat
    12541 agaaccagaa cgcgcacggc cggtgccttt ctggcgccac ggttttttac cggaaccagt
    12601 tacttcagca cgagtcttct gagcacgagt accctgacga gcaccagctg cataagcaac
    12661 aacaacctgg tgaaccagcg cttcgttgaa atcacgaccg aaggtagttt cggaaacagt
    12721 cagcgcgctc tgcgcgtctt tcaatactaa ttccattgct atctccttac gccttcacag
    12781 ctggtttaac gatcaggtcg ctaccggttg cacccgggac agcaccttta accagcagca
    12841 ggttgcgctc agcgtcaacg cgtactacgt caaggctctg aacggttaca cgttcgttac
    12901 ccatctgacc tgccattttc ttgcctttga acactttgcc cggagtctgg ttctgaccga
    12961 tagaacccgg aacgcggtga gacaaggagt taccgtgagt agcgtcctgg gtacggaagt
    13021 tccagcgctt aacggtacct gcgaaacctt tacctttaga ggtgccagtt acgtcaactt
    13081 ttttaacgtc agcaaacagt tcaacgctaa tgctctgacc tacagtgaac tcttcgcctt
    13141 cagccaggcg gaattcccac agaccacggc cagcttctac gccagcttta gcgaagtggc
    13201 cagcttcagg cttggtcaca cggttagctt ttttagcacc ggtggtcacc tgaatagcac
    13261 ggtagccatc gttagccagg tctttaacct gagtaacgcg gtttgcttca acttcgatta
    13321 cggttactgg gatagaaacg ccgtcttctg tgaagatacg ggtcataccc acttttttac
    13381 cgactaaacc aatcattgtt tcaacctctc aatcgctcaa tgacctgatt aacccaggct
    13441 gatctgcacg tctacaccgg cagccagatc cagacgcatc agagcatcaa cggttttctc
    13501 ggttggctca acgatgtcaa ccagacgcaa gtgagtacgg atttcgtact gatcgcgcgc
    13561 gtctttgttg acgtgcgggg agatcagaac agtgaagcgc tctttgcgtg tcggcagcgg
    13621 gatcggacca cggacctgcg caccagtgcg cttggcagtc tcgacgattt ccgcggttgc
    13681 ttgatcgatc agacgatgat caaacgcttt caggcggata cggattcttt ggttctgcat
    13741 gagaccagag ctccaattat tttataaacg aaaatgatta ctcctcagac ccattacgat
    13801 tgatgggaga gtgtaaccgt tcttacgtag ctccccgatt gggagcattg ttaggtagcc
    13861 aaattcggct aactgaggtt cagattgaac ctgctgtcaa ctacgacaag cccgcgcatt
    13921 atacgcaaat ctgagcctga cgcaagcatc gggtagaaat taatcgcaac gcgcagattg
    13981 cgagctgcat tccacgaaat ttgtaaggtt gtgaagatag cgtttttcac cggaatcagg
    14041 ttaacaaatc agcgctctct ctctgttgct gagcatccgg agttattcga tggtgatact
    14101 ccaattacag aggatgtttt atggacacta cccttccctt tctaattttg tacgcttgtt
    14161 tatcagccct actttttttc tgggatgcaa agcatggctt gttacctgac agatttacgt
    14221 gcccgttact ctggtcaggt cttttgttct atcaagtttg tcatcctgat ggtttagccg
    14281 atgcgttatg gggggcaatt gttggttacg gcacattcgc tgtcatttac tgggggtatc
    14341 gcatactgcg acataaagaa ggattaggct atggtgatgt gaagtttctc gccgcccttg
    14401 gcgcctggca ttcatgggcc ttcttaccac gattggtttt ccttgctgcc tcattcgctt
    14461 gcggagccgt agttattggt ctgctcatga gaggaaaaga atcattaaaa aacccgctgc
    14521 cttttgggcc atttctggcg gctgcgggtt tcgttatagg ctgggatagt ttgctgatag
    14581 gccgttaatc tttaacttta atttgtgatt gcaggtaatt ttgtaggccg attttaccga
    14641 tgagatccag ttcagtttcg agccagtcga tatgaccttc ttcatcagca aggatctcga
    14701 tcatcatatc gcggctaaca tagtcatgaa cgctgtcggc atatgcgata gcctcacgca
    14761 gatccttcgc gccttccagt tctaatcgca ggtcagattg cagcatctcc tcgacatctt
    14821 caccaatccc cagtttgcct aaatcctgta agttggggat tccttctaaa aacaaaatac
    14881 gctcgatata tttatcagcg tgtttcatct catcgatgga ttcatggtat tcgacgtcat
    14941 tgagacgcat caggccccag tttttaaaca tccgggcatg gagaaaatac tgattgattg
    15001 cgacaagctc atttcccaat agtttattga gataatttat gattttaaca tcacctttca
    15061 ttttatagtc cctccgcttc cactattaga gcgtagatgg ggctagcagg atgtcaaaaa
    15121 ataagcgtgg gcttcaggca atctctttaa attctggcat ctgcatcaac tcgtcctgca
    15181 tcacttcgcg cgcggcgcga atgcacttac cgcattgatt tcccacagga ataaacttac
    15241 gcaattgttg gaaagattgc ggatgaaact gacggactgc ctggcgaatt tttttgtcac
    15301 ttacaccatt acacaaacaa acgtacatga ttgctcccgt tcaaattctg cacaaagtgt
    15361 aaatgagaat ggttatgatt acaatagtac agttttattt gcgccatagt tcagcgagca
    15421 aaattacgac taaatatttc gagctcaaat tacgggcagc aaaaagggcg ccgaagcgcc
    15481 cttttcaatt caaaactaat taacgtgtaa ttagcccaga actttagcaa caacgcccgc
    15541 gccaacggta cggccgcctt cacggattgc gaaacgcaga ccgtcgtcca tcgcgatcgg
    15601 gtggatcagg gtaacaacca ttttgatgtt gtcgcccggc attaccatct ctacgccttc
    15661 cggcagttcg atggtaccag tcacgtcagt agtacggaag tagaactgcg gacggtagcc
    15721 tttgaagaac ggagtatgac ggccgccttc atctttggac agaatgtaca cttcagattc
    15781 gaacttggtg tgcggcttga tggtgcccgg cttagccagt acctgaccac gttcgatttc
    15841 ttcacgtttg ataccacgca gcagaacacc tacgttctca ccagcacggc cttcgtccag
    15901 cagtttgcgg aacatttcaa cgccagtaca ggtagacttc tgagtctctt tgataccaac
    15961 gatttcaact tcttcaccaa ctttgatgat accgcgttct acacgaccgg taacaacggt
    16021 accacgaccg gagatggaga atacgtcttc gatcggcagc aggaacggct tgtcaatcgc
    16081 acgctctggt tccggaatat aagaatccag gaagccagcc agttccagga ttttcgcttc
    16141 ccactctgcg tcgccttcca gcgctttcag agcagaacca cgaacgatcg gagtgtcgtc
    16201 gcccgggaag tcgtactgag acagaagttc acgaacttcc atttcaacca gttccagcag
    16261 ctcttcgtca tcaaccatgt cgcatttgtt caggaacacg atgatgtacg gaacgcctac
    16321 ctgacgaccc agcaggatgt gctcacgagt ctgcggcatc gggccgtcag tcgcagcaac
    16381 taccaggatc gcgccgtcca tctgagcagc accggtgatc atgtttttaa catagtcggc
    16441 gtgccccggg cagtctacgt gtgcgtagtg acgggtcggg gtgtcatatt caacgtgaga
    16501 agtgttgatg gtgataccac gagctttttc ttccggcgcg ttatcgatct ggtcgaatgc
    16561 acgagcagca ccgccgtagg ttttagccag tacggtggtg attgcagcgg tcagagtagt
    16621 tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg tgcggttttg tacgttcaaa
    16681 tttttcttta gacacggcta tattccttac tatagtgctc tccccttcag gagagagcac
    16741 gggactttgg tattaaccct taggcttatt taccacgggc ttcaattacg gcctgagcaa
    16801 cgttactcgg cgcttcatca tacttcagga attccatagt gtatgatgca cgacctttgg
    16861 tcagagaacg cagctgagtt gcgtatccga acatttcaga cagcggtact tcagcgtgga
    16921 tcttaacgcc agtaacttca gattcctgac ctttgagcat accacgacga cggctcaagt
    16981 caccgataac gtcaccggtg ttctcttccg gagtttctac ttcaaccttc atgatcggct
    17041 caagcagaac tggtttcgct ttcttaaagc cttctttaaa ggcgatagaa gcagccagtt
    17101 taaacgccag ttcagaggag tcaacgtcat ggtaagaacc gaagtgcaga cgaataccca
    17161 tgtctactac cgggtagcct gccagcggac ctgctttcag ctgttcctgg atacctttat
    17221 caacggccgg gatgtattcg ccagggatta caccaccttt aatgtcgttg atgaactcgt
    17281 agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc gataacaaca tgaccatact
    17341 gaccacgacc accagactgt ttcgcgtgtt taccttcaac atcggtaact ttctggcgga
    17401 tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc ttcaacgttg aattcacgct
    17461 tcatacggtc aacgatgatg tcgaggtgca gttcgcccat acccgcgatg atggtctggt
    17521 tagattcttc gtcagtccat acacggaaag acgggtcttc tttagccaga cggcccagag
    17581 ccagacccat tttttcctgg tcagctttgg ttttcggttc aactgcgatg gagattaccg
    17641 gctcagggaa ttccatacgt tccagaatga tcggcgcatc cgggtcacac agggtgtcac
    17701 cagtggttac gtctttcaga ccgatagcag cagcgatgtc gcccgcgcga acttctttga
    17761 tctcttcacg tttgttagcg tgcatctgaa cgatacgacc gaaacgctca cgtgcagctt
    17821 tcacggagtt cagtacggta tcaccagagt taaccacacc ggagtaaaca cggaagaagg
    17881 tcaggttacc aacaaacggg tcggtagcga ttttgaacgc cagtgcagag aacggctcgt
    17941 catcacttgc gtgacgttca gccggagtgt ctttaccgtc gtccaggata ccgttgatcg
    18001 caggtacgtc aaccggggat ggcaggtaat caattaccgc atccagcatc gcctgaacac
    18061 ctttgttctt gaacgcagaa ccacaggtta ccaggatgat ttcgttgttc agaacgcgct
    18121 gacgcagagc acctttgatt tctgcttcag tcagttcttc accacccagg tatttttcca
    18181 tcagctcttc agaagcttca gctgcggatt cgatcaggtt ctggtgccat tcgttagcca
    18241 gttcaaccat gtctgccggg atatcttcgt attcgaaggt tacgccctgg tcagcgtcgt
    18301 tccagttgat agctttcatt ttcaccaggt caacaacacc ggtgaaatgt tcttcagcac
    18361 caatcgccag ctgcagcgga accgggttcg cgcccagacg ggttttgatc tggttaacaa
    18421 ctttcaggaa gttcgcaccc acgcggtcca ttttgttaac gaacgcaatg cgcggaactt
    18481 tatatttgtt tgcctgacgc catacggttt cagactgcgg ctgaacacca ccaactgcgc
    18541 agtaaaccat taccgcacca tcgagaacac gcatggaacg ttctacttcg attgtgaagt
    18601 caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg ctcatactgc ttagccatac
    18661 cagaccagaa tgcagtagtc gcagcggaag tgatggtaat accacgttcc tgctcctgct
    18721 ccatccagtc catggttgca gcgccgtcat gaacttcacc gattttatgg tttacaccgg
    18781 tgtagaacag aatacgttcg gtagtagtgg ttttaccggc gtcgatgtgc gcactgatac
    18841 cgatgttacg gtagcgtgcg atgggtgttg tacgagccat ttgtttcctc gtttatcttt
    18901 taggcgttca atttaagtag cccaaagcgg gctgcttact ggaagcgccc gcctggtgac
    18961 taaaactccg aagggattac caacggtagt gtgcgaacgc cttgttggct tcggccatac
    19021 ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt ttctgcagca tcagaaagtt
    19081 cgttcgccag gcgcagagcc atggatttat caccgcgttt acgagcagct tcaacgatcc
    19141 aacgcattgc cagagcatta cgacgaaccg gacggacttc aactggtacc tgataagtag
    19201 aaccaccaac gcggcgagac ttaacttcta cagtcgggcg cacgttttcg agagctactt
    19261 cgaatgcttc cagttcagat ttaccagagc gctgagccag ggtctccagc gcgctgtata
    19321 cgatagattc agcagtagat tttttaccat ctaccatcag gatatttaca aatttagcca
    19381 gcagttctga tccgaacttc ggatccggca gaattttacg ctgaccaatg acgcgacgac
    19441 gtggcatgga aatactccgt tgttaattca ggattgtcca aaactctacg agtttagttt
    19501 gacatttaag ttaaaacgtt tggccttact taacggagaa ccattaagcc ttaggacgct
    19561 tcacgccata cttggaacga gcctgcttac ggtctttaac gccggagcag tcaagcgcac
    19621 cacgtacggt gtggtaacga acacccggga ggtctttaac acgaccgcca cggatcagga
    19681 tcacggagtg ctcctgcagg ttgtgacctt caccaccgat gtaggaagtc acttcgaaac
    19741 cgttagtcag acgaacacgg catactttac gcagcgcgga gttcggtttt ttaggagtgg
    19801 tagtatatac acgagtacat acgccacgtt tttgcgggca tgcttccagc gcaggcacgt
    19861 tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag ctggttaact gttgccatta
    19921 aatagctcct ggttttagct tttgcttcgt aaacacgtaa taaaacgtcc tcacacaata
    19981 tgaggacgcc gaattttagg gcgatgccga aaaggtgtca agaaatatac aacgatcccg
    20041 ccatcaccag gccatctggc tggagtgctt aaccgtaagt ctgacgaaat cagtatagtc
    20101 aatgggaatg atgtcgttca aaatttgaac agtcaaaccg cgggcaataa ggtcttcgtt
    20161 cagggcatag accttaatgg gggcattacg cagactttca aggtagcggt taccgtcaac
    20221 tgcggcagtt acgccatctt gcaataatag cagttcgtct ccttcactga gcagacgcag
    20281 cagcgcagca aaatccgtca gccagggtga acgatgtaac gtgtgcagca taaacgcctc
    20341 aaaacctcaa aataacatcg tagttggcga gttcgcggcg taaggcatct gcttcgagcg
    20401 gcgtggcttc gacaacaaag ggtgtctgcg gatctaaccc gcgttcgcgc agtgaagccg
    20461 cacaaaccca gcactgttca atgtcgtaca gactcaacaa tttaaaagtg gcaatgtaat
    20521 cacgcgccag cactgcatcg ggcttttgtc ctgacagcag ctgaaaaacg ccatcagcaa
    20581 taaagaagac ggccagattg tcagttaatg cggaagttgc cagtaaagca tctaaacctt
    20641 cccgaccagc agctgtacca tgaggtgcag tagaaaaaac aaacgcaatt cgtttcatca
    20701 gaactgtacc accctgtcac aggtcagcga ggcttccgcc agcgcgccaa gtccgcttaa
    20761 ggtaaatccc ggctgaaggt ttgacgaagc cagccccagc cttcctgctt cagtttcatc
    20821 gacgataccg cggcgtaacg ctgccgctac gcagatattc agcgccacac catgttgcgc
    20881 attcaactgt tgccagccgc gcacaaggtc aaattcgtcg cttgccggtg aggtcagttg
    20941 gttagcgtta tagacccctt cccgatagaa aaagacgctg cttaacttat ggccttctgc
    21001 tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt tgcgtaccgt atgctggccc
    21061 ggtcaccacg atggcaaaac gcattactta tcttgcccct gaaaatcacc gctcttgaac
    21121 tggcggatgt agagatagac agtgtgtttg gagatgttca ggcggtcagc aacctggttg
    21181 atcgcatctt taatatcgaa gatccctttc tcgtagagat tcagcacgat ctgacgattt
    21241 ttggcgttat tagaaacatt gcgatcggca ttcacttctt cgatggtgaa ctccagcgtt
    21301 tgggtaacca gatcttcaac agaagaggca aagttgacgc ttgaaccgac atccggggtt
    21361 tctggcggca caaaagtgct cataatctgc gagaagggaa catcaagatt catattgatg
    21421 cacagcagac caattacacg ctgttcgcgg ttacgaatcg cgatagtcag ggacttcatt
    21481 aatacgccgc ttttggcgcg agtaaagtag catttagaaa cgctgctatc cgctcccgtc
    21541 atatcgtgca gcatacgtag cgccaggtca gtaattggcg aaccaatctt ccggcctgta
    21601 tgttcaccgt tagcaatgcg aatggctgaa cattttagat cctgcaaaga gtgcaaaacg
    21661 atttcacagt gggagccaat aagcatcgct aacccgtcca ccaccgcttc gtaggatttc
    21721 agaatatcaa aatcggtctg gtcgaaagga cgttgatcca gtaaatccaa ctcactggtt
    21781 tcgttggtta aaagcgacct ggacatgaaa aaaaacactc cttttcagga gcctgtcgtt
    21841 aacttttcag ggcaggctca ttaatgatgc gggtaactaa attaatacag cggaggttcc
    21901 gctttccagc actaattata tccggcctgt aataaaaaaa ccgccgcctg gtcaggcggc
    21961 ggttcttaat gcttattttt tagcagaatc tgcggctttc gcatcagctt ccggctttgc
    22021 atcagccttc ggcgctggtt tcacatccag cagctctacg tcaaacacta gggtagaatt
    22081 cggtgggatc cccggaacac ccgctttgcc gtaagccagt tctggtggaa taaccagttt
    22141 gatcttaccg cctttcttga tgttcttcag accttctgtc caacccggga taacaccgtc
    22201 cagacggaaa gaaagcggtt caccacgggt gtaagagttg tcgaactctt taccgtcgat
    22261 cagcgtacct ttgtagttca ctacaacagt atcgctgtct ttcggtgctt cgcctttacc
    22321 ggcttctact acctgataaa ccagaccagt tgaagaggtt ttcacacctt tctctttggc
    22381 aaatttctcg cggtactctt taccttttgc ttcgttatca gccgcgtctt tttccatctt
    22441 cgcctgagca gaagacttca cgcgagcttc gaatgcttgc agagtctgtt cgatctcttg
    22501 gtcggagagt ttgctcttat cagcaaatgc atcctgaaca ccagcgatca gctgatcttt
    22561 atccagtttg atgcccagtt tttcttgttc tttaagagag ttttccatgt aacgacccag
    22621 cgaagcaccc agtgcataag ctgatttctg atcgtcattt ttgaacgatg ctttgctgtc
    22681 tgcagttgta gcaggttttg cagcttcagc agcaaaagtg attggtgcat gcagggcaac
    22741 ggccattgtg gtcgccagta gcgttacttt aaacagtgat ttcatccata tctccaggat
    22801 cggggcatct caccccaggg ttaactatta tcagaagggt actataaagc gttgtcgaac
    22861 aaatctacat acagacacgc cctattatca tctattttca gactcttttt gtttaaatta
    22921 gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa agttaagtag aatccgcggc
    22981 gaccattcga caaaagaggt gaatcatgca ggatttatca ttggaagcac gcctggcaga
    23041 gctggagagc cgactggctt ttcaggaaat caccattgaa gaactgaacg tcacggtgac
    23101 cgctcatgaa atggagatgg cgaaactgcg cgatcatctg cgtctgctga ccgagaagtt
    23161 aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa gaaacgccac cgccacatta
    23221 ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc accttttcgg tattagtggc
    23281 aaccacaacc gccgttgcct ttaccgccac agcagccttc gccaccgtgt tcatgaccgt
    23341 gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg atcgtggtga tgatcgtgcg
    23401 cgccgtgaac gtggccatga gccagttctt cttcggtcgc ttcgcgaatc gccacaactt
    23461 caacgttgaa tttcaggttc tgaccggcca gcatgtggtt accatcaacc actacgtgat
    23521 cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg gtcagtttca gccaggaaac
    23581 gcatacctac ctgcagttca tcaacgccca taaatacgtc tttaggaaca cgttgcacta
    23641 ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac agcgacatca aatttgtcgc
    23701 caacttcatg accttccagc gccgtttcca ggccagagat cagggaaccg tgaccatgca
    23761 ggtagtccag cggcgcactc accggagact catcaaccaa cacaccgtct tctgtacgta
    23821 cctgataggc caggctgacc accaggtctt ttgctacttt catgatatct cctgagcatg
    23881 ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc gtgtactcac tagcttaaaa
    23941 aaactcggcg catatcgcta gtccggatga aaaatcccga tcacttgctc atctttgtga
    24001 acgtgatcgc gggcttcttt gtctgcttct cgcatctgat gtccgcactt aacacattca
    24061 acaatatcaa tattattttc gcgccacatc gccattgaat cctgcgcctg acaggccggg
    24121 cattttgcgc ccgctataaa acgttttcgg attgccatcc ttaccctcta ctcaaattca
    24181 tcccagccgt ccagctggcg tcgttcttgt tgcatttcac gctggaaaat ctcttccagt
    24241 tcgcgtcggg cttccctggc gcgagaaatt tgtacggtat cagcatgcat cgggattaac
    24301 tcccgcagca ttcgcatatc caggcggcga aaatgcagtt gcgcccgctg tgcctgatgc
    24361 ggatgcatgc caagcgtgac cagcgtcttg cgccccagct ctaacgcact ggagaatgtt
    24421 tcacgggaaa actgcgtcac ccctgcctgt aataactcat gcgcttccac acgtccgcgc
    24481 gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc atatttccac cagcttcatg
    24541 gtgtcttccg gctcgttaca ggtaatgacg atagactcag cggcctctgc acccgcagaa
    24601 cgtaaaagat cgacctgcgt ggcgtcgccg taataaactt tgtagccata tttacgcatc
    24661 aggttaacgg cgctgatatc ccgctccagc acggtaatgc gcattttatt tgccatcagc
    24721 aaacgaccaa tcacctgacc aaaacgcccg aagcccacga caatgacctg gggtttatca
    24781 tcgttgaccc acggtttttc gtcttcttct tccggtccgt taaactggcg ggatagccat
    24841 ttatccacca gcttcatcag caacggcgcc atctggtcgc cctggaataa acgttgtgaa
    24901 gaagcggtag aaaagaggac aaaggcaaac tcaccgccct gactcaacac gccagcaaac
    24961 tgcatccgct ctgagctacg cacgccatac aatcgcgcca gcagatacag cacgagaatt
    25021 ttcaccgcca ccagcacaac cacgcttatc actacccaca acagatgggt ataaagcacc
    25081 ccgaggttga gcgacatgcc gacagagata aagaacaaac cgagcagcaa gcctttgaag
    25141 ggatcgatag ccgtttccag ttcatggcga tattcacttt ccgccagcag cacgcccgca
    25201 ataaatgtac cgagcgccat cgacagcccc agcgcatcca taaacaatgc ggaacccaac
    25261 accagcagca gcgtcgcggc ggtgaacact tcccgcacgc cagaagctgc aataaagcgg
    25321 aataccggac gcagtaaata gcgcccacca atcagcatgc cgacaaactc cagcaccttc
    25381 atgccgatct tcatccagtc gaaatgttcg tctgccgacc ccgccaacaa cggcactaac
    25441 gccagtgctg ggatcactgc cagatcctga aacagcagaa ccgaaaaccc gagctggccg
    25501 gattcgctgc gattcatccc tttctcacgc atcaattgca acgccattgc agttgaagac
    25561 atcgcaaagc caatgccacc gaccaccgcc gcctgccagg cgaaatccgt cagcatcaat
    25621 aatcccgcca gcaacgccgc gcttaacagc acctgtgccg cgcctacgcc aaaaatcgaa
    25681 cgccgcagtt gccaaagttt ggagggattc aactcaaggc cgatgataaa catcaggaat
    25741 accacgccga gttccgaaaa gtggaggatc tcatcgacgt cgctaataaa ccccagcccc
    25801 cacgggccaa ttgcaatccc cgccagcaaa tatcccaaca cagcgccaat ccccagccgc
    25861 gaagagaaac agcactcctg cgagtaaaaa atcggaacct tccatcagcg gcctcctgga
    25921 gacagcggat ttgccaacca gtcaccgtag gctctggcgt ggctcgccag ctcctttgcg
    25981 ctttgccgtc tcgcccagta aataatgatg ggacttaacc aatgcatccg gcacatgccc
    26041 gccgccagtt caaaggggcg cagcacatcg ctcatcgggt agcgattcag cgcgtcataa
    26101 cggtaagcac tttccggctc gccggtggta atcacgttac gccagtactt tcccgccagt
    26161 tggtttcctc ccggcccgct ggcaaaacca cgacttaata cccggtccag ccactctttc
    26221 agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac ctcgtgctcg
    26281 cgcagtaatg cctgctcacg ggggatatca ataaaaaaat cgggatagtg cgcgtaaagg
    26341 tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagtacccg gtttgccacc
    26401 gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg agacatcatc
    26461 cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg cgcccggtgc
    26521 ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct gaacatacgg
    26581 cgccttatga ttgttttctc ctcgttacaa attcgtcgcg gcgtgcgcgt cctgctggat
    26641 aatgccaccg ccaccatcaa ccccgggcag aaagtcggcc tggtgggtaa aaacggctgt
    26701 ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg cggcagctac
    26761 acctttccgg gaagctggca actggcgtgg gtgaatcagg aaacgccagc gttaccgcaa
    26821 gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga agcgcagctg
    26881 cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatag caagctggat
    26941 gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg cctcggtttc
    27001 agcaatgaac aactggagcg cccggtaagt gatttctccg gtggctggcg tatgcgtctt
    27061 aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga accgactaac
    27121 cacctcgatc tcgatgccgt tatctggctg gaaaaatggt tgaagagcta tcagggcacg
    27181 ctgatcctga tctctcacga ccgcgacttc ctcgatccga tcgttgataa aattattcat
    27241 atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga agtacagcgc
    27301 gccacccgtc tggcgcagca acaagcgatg tacgaaagcc agcaggaacg cgtggcacat
    27361 ctgcaaagtt atatcgatcg tttccgtgcc aaagccacca aagcgaagca ggcccagagc
    27421 cgcattaaga tgctggaacg tatggagctg attgcccccg cacacgtcga caacccgttc
    27481 cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat ggaaaaggtc
    27541 agcgcaggct atggcgatcg cattattctc gactcgatta aattgaatct ggtccccggc
    27601 tcgcgcattg gtctgttagg ccgcaacggc gcaggtaaat cgacattaat caaactgtta
    27661 gccggtgaac ttgcgccagt cagcggtgaa attggtctgg cgaaagggat caagctcggc
    27721 tacttcgccc agcatcaact tgaatacctg cgcgccgacg aatcacctat tcaacatctg
    27781 gcacgtttag cgccgcagga gctggaacaa aaactgcgtg actacctcgg cggctttggt
    27841 ttcctgggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga aaaagcccgc
    27901 ctggtgctgg cattaattgt ctggcagcgt ccgaatctgc tgctgctcga cgaaccgact
    27961 aaccaccttg acctcgacat gcgtcaggca ctcaccgaag cattaatcga gtttgaaggc
    28021 gcgctggttg tcgtttcgca cgaccgtcat ttgctgcgtt ccaccactga cgatctctac
    28081 ctggttcacg atcgtaaagt cgaaccgttc gacggcgatc tggaagatta tcaacagtgg
    28141 ttgagcgacg tacaaaagca ggaaaaccag accgacgaag cgccaaaaga gaacgcgaac
    28201 agcgcccagg cacgtaaaga tcagaagcgt cgggaagcgg agctgcgtgc gcaaacccag
    28261 ccgctgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa cgcgcaactg
    28321 gcgcaggcgg aagagaaact cggcgacagc gaactgtatg accagagccg taaagcggag
    28381 ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga gtgcgaaatg
    28441 gcgtggctgg aagcccagga gcagcttgag cagatgctgc tggaaggcca aagcaactga
    28501 tggcgcagat aacgacgacc gatgccaatg aatttagcag cagtgctgaa ttcaccccta
    28561 tgcgcggctt tagcaattgt catctacaaa ctatgctgcc gcgtttgttt cgtcgtcagg
    28621 tgaaattcac cccgtactgg cagcggctgg agttgcccga cggcgatttt gtcgatctcg
    28681 cgtggagtga agaccccgca caggcgaaac ataaaccgcg tttagtggtg tttcacgggc
    28741 tggaaggcag tctcaatagc ccttacgccc acggtctggt tgaggcggcg caaaagcgcg
    28801 gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac cgtatgcacc
    28861 gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg ctgcaacgcg
    28921 aatttggtca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat atgctggcct
    28981 gtttgctggc aaaggaaggc aatgatctcc cggttgatgc ggcggtgatt gtctctgcgc
    29041 cgtttatgct ggaagcctgt agctatcata tggaaaaggg cttttcccgc gtttatcagc
    29101 gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc taccccggaa
    29161 cgctgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa tttgacgatc
    29221 taatcaccgc cagaattcac ggctacgccg acgctatcga ctattatcgt cagtgtagcg
    29281 ccatgccaat gctgaaccgg atcgccaaac cgacgctgat tattcacgcc aaagacgatc
    29341 cgtttatgga tcatcaggtg atcccaaaac cggaaagtct cccctcgcag gtggagtatc
    29401 aactgactga acatggcggt catgttggct ttattggcgg tacattactt catccgcaaa
    29461 tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg aaatcatgtt
    29521 gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa gctttgtgtt
    29581 acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag tcgccgacgt
    29641 caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc acgaaacggt
    29701 caatattatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg gacgagataa
    29761 tagtgatagc taagtcatgg agttactatg tctgccaaac atccggtcat tgcggtaaca
    29821 ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat attcgcgcag
    29881 ttaaatctgc atgcagctga ggtggaaggc gacagttttc accgttacac ccgcccggaa
    29941 atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta cttcggcccc
    30001 gaagcgaacg acttcggcct gctggaacaa accttcattg aatacggtca gagcggcaaa
    30061 gggaaatctc gcaaatatct gcatacctac gacgaagccg taccctggaa tcaggtaccg
    30121 ggaacattca ccccctggca acctttacca gaacccactg atgtactgtt ttatgaaggt
    30181 ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga cttactggtc
    30241 ggcgtagtgc ctatcgttaa ccttgagtgg attcaaaaac tgatccgcga caccagcgag
    30301 cgcgggcact cacgagaagc agtgatggac tcggtagtgc gttcaatgga agactatatc
    30361 aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt tcccaccgtc
    30421 gacacttcaa acccgttcgc ggcaaaaggt atcccgtcgc tggatgaaag ctttgtggtg
    30481 atccattttc gtaatctgga agggatcgat ttcccttggc tgctggcgat gttgcaaggc
    30541 tcattcattt cccacatcaa tacgttagtg gtacctggcg gtaaaatggg gctggcaatg
    30601 gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat cgagtaactt
    30661 tgctattatg ccggataaaa tactatccgg cttcacaacg ggatagttaa gtcacgcggc
    30721 aaccacttca tacgagtgag taatattcac cgctttttcc agcatcaacg ccactgaaca
    30781 atatttctcg gcagagagat caaccgcacg cgcaaccgct gcgtctttca ggtcgcgacc
    30841 ggtgacgata aaatgcagat taatgtgcgt aaacaggcgt ggtgcctctt cgcggcgttc
    30901 agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca ggatcgaaac
    30961 cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg gacttggtgc
    31021 tttatcgcct gagttgccgt ccattaaaat ctggtgacca gaggcggatt cgcccagaaa
    31081 agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac tgcgtcaatt
    31141 ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct gggtcatgct
    31201 gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag gatgctacag
    31261 taatacattg atgtactgca tgtatgcaaa ggacgtcaca ttaccgtgca gtacagttga
    31321 tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc ggcgttatct
    31381 ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca aaccgcaaac
    31441 agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc catccaagag
    31501 cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta aaggctctgt
    31561 ggcagtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc tgaatcaggg
    31621 tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg catgggtacg
    31681 tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc aattgattca
    31741 ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc tgcaagtcac
    31801 ttcagagaaa gtgggcaacc tggcgttcct cgacgtgacg ggccgcattg cacagactct
    31861 gctgaacctg gcaaaacaac cagatgctat gactcacccg gacggtatgc aaatcaaaat
    31921 tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg gacgcattct
    31981 gaagatgctg gaagatcaga acctgatctc cgcacacggt aaaaccatcg tcgtttacgg
    32041 cactcgttaa tcccgtcgga gtggcgcgtt acctggtagc gcgccatttt gtttcccccg
    32101 atgtggcgca gactgattta tcaccccgat atcaactatg cacttcgaca aacgctggtg
    32161 ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg tctgctcttc
    32221 tccctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa acgttttttc
    32281 aaacacttaa tcattggtgc gtcgctgttt gccacctgta gcttactgac acagctgcta
    32341 ctggtaaaag atgttcccct gccctttttg ctgaccggat taacgctggt acttggcgtc
    32401 actgctgagc tggggccatt gcacgcaaaa ttgcttcctg catcgctgct cgccgccatt
    32461 tttaccctca gtctggcggg atacatgccg gtctgggaac cattgctcat ctatgcgttg
    32521 ggcactctct ggtacggatt gtttaactgg ttttggttct ggatctggcg cgaacaaccg
    32581 ctgcgcgagt cactaagtct gctgtaccgt gaactggcag attattgtga agccaaatac
    32641 agcctgctta cccagcacac cgatcctgaa aaagcgctgc caccgctgct ggtgcgccag
    32701 caaaaagcgg tcgatttaat tactcagtgc tatcagcaaa tgcatatgct ttccgcgcaa
    32761 aacaataccg attacaagcg gatgctgcgt attttccagg aggcgctgga tttgcaggaa
    32821 catatttcgg tcagtttgca tcagccggaa gaggtgcaaa aactggtcga gcgtagccat
    32881 gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg cgtgctggct
    32941 gatgacattc tttaccatcg cctgccaacg cgttttacga tggaaaagca aattggcgca
    33001 ctggaaaaaa tcgcccgcca gcatccggat aatccggttg ggcaattctg ctactggcat
    33061 ttcagccgca tcgcccgcgt gctgcgcacc caaaaaccgc tctatgcccg tgacttactg
    33121 gccgataaac agcggcgaat gccgttgctt ccggcactga aaagttatct gtcactaaag
    33181 tctccggcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc cagcctgatg
    33241 ggcaccgtgc tgcatctgcc gaagtcgtac tggatcctga tgacggtatt gctggtgaca
    33301 caaaatggct atggcgcaac ccgtctgagg attgtgaatc gctccgtggg aaccgtggtc
    33361 gggttaatca ttgcgggcgt agcgctgcac tttaaaattc ccgaaagtta caccctgacg
    33421 ttgatgctga ttaccaccct cgccagctac ctgatattgc gcaaaaacta cggctgggca
    33481 acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct gaacggtgag
    33541 cagtacatcc ttccacgtct tatcgatacc attattggtt gtttgattgc tttcggtggt
    33601 accgtctggc tgtggccgca gtggcagagc gggttattgc gtaaaaacgc ccatgatgct
    33661 ttagaagcct atcaggaagc gattcgcttg attctgagcg aggacccgca accaacgcca
    33721 ctggcctggc agagaatgcg ggtaaatcag gcgcataaca ctctgtataa ctcattgaat
    33781 caggcgatgc aggaaccggc attcaacagc cattatctgg cagatatgaa actgtgggta
    33841 acgcacagcc agtttattgt tgagcatatt aatgccatga ccacgctggc gcgggaacac
    33901 cgggcattgc cacctgaact ggcacaagag tatttacagt cttgtgaaat cgccattcag
    33961 cgttgtcagc agcgactgga gtatgacgaa ccgggtagtt ctggcgatgc caatatcatg
    34021 gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca gcatttacag
    34081 cgggttattg gccatctgaa caccatgcac accatttcgt cgatggcatg gcgtcagcga
    34141 ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaaggcgta atacaggcta
    34201 aaccatcgga taaggcattc acgccgcatc cgacatattt tgcctgatac gtcttattta
    34261 cgccccaacc accttcgcca ccgcgtgggc gaaacgttgc atcccttcat cgatatccgc
    34321 atcttccacc accagcgacg gtgctaaacg catcacatcc ggtccggcat tcagcaccat
    34381 tacgccaacc tctgcacccg catacaggaa atcacgcgcc cgacctttgt agtgtggttt
    34441 cagctccgcg ccaattaaca gccccatacc gcgaatatcg ctaaacacat cgtactgctg
    34501 atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatgc cttccagcac
    34561 ttcaggggta ttgatgatat caaacgccgc gcccgctact gcacaggcca gaggattacc
    34621 gccgtaggtg gaaccgtgag aaccaggatg aaacgcagaa gcaatttccg ccgtggtcag
    34681 catggcgcta atcgggaagc cgccgcctaa cgctttcgca gaagtcagaa tatccggcgt
    34741 cacaccgtag tacctgtaag caaacaaatc gccagtccgc cccatcccgc actgcacttc
    34801 atcaaacacc aataatgcct gatgttgatc gcacagctcg cgcaagccct gcaaaaactc
    34861 tggcgtcgct gccgtcacac cgccctcgcc ctggatcggc tcaaccacca ccgcacaggt
    34921 gtgatcatcc atcaccgctt tcactgcatg gagatcgtta aagggaacgt ggatgatgtc
    34981 tgccggtttc ggcccaaagc cgtcggaata ttttggctgc ccacccaccg aaacggtaaa
    35041 cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga acgggctatg
    35101 acgcacacag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt ccgtgccgga
    35161 gttcataaac accacgcgtt cggcaaacgt cgcctcaatc agtttgcgcc caagacgcag
    35221 cgccggttca ttggtgaaaa cgttactgat atgccacaga gtttcgccct gggtttttaa
    35281 cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc cacccgcgaa
    35341 atcgacatac tccttgcctt gctgatccca gattcggctg ccctgacctt ttaccggaat
    35401 aaactctgcc ggagcataaa tcggcaggat cacttcatcg aaagtcgcgc gtgtaattgc
    35461 tgtttgttca attgccatct catgatcacc ctgttacgca taaataaatg tgaaattata
    35521 atcacaaaat atgcataaaa aatcactgaa tggcaatcgg aaatcagaga tgcaggaaat
    35581 tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaac tgcacacctt
    35641 ccagatccca ctggcgatgg cgaatcccca taatctctcg ggtttcgctc caggccgtca
    35701 cgtcaaagca cgctggtaat gagtcaggtt ccaccaccag cgaatggtag cgtgtcacgg
    35761 taagtggatt tgccagcccc cgaaatacgc cctcaccgct atgtgtaatc ggcgaggttt
    35821 tgccgtgcat gacctttgcg gcgcgcacaa ctttgccgcc aaatgcctgc gccattgcct
    35881 gatgaccgag gcagacgcca agaatcggca agcgcccggc atagtggcga ataacgtcaa
    35941 gggagatccc ggcttcatct ggcgtacagg ggccaggtga gatgacaatt ttttgtggtt
    36001 taagggcgtc gatatccgcc agcgtcaacg catcgttgcg cttaaccagc acatccgccc
    36061 ccagttcaca aaagtactgg tagaggttcc aggtaaaaga atcgtagtta tctataagca
    36121 ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc cccggcttcg
    36181 cttaccactt tgctaaatat catctgcagt gcggtgagat cccccattgc cccactctga
    36241 tttgcctgat tccaggcctc tttttcgata ccctgccagc tcagttgata tccggcatga
    36301 atcgccagtt gctcgaagaa gatccgctgt gccagaccac ttcccacccg gaagggatgc
    36361 agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgc tttctccagg
    36421 ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc tttttcgata
    36481 taagcaaagt ggcagaacgg cgtatcgccc tgataaatat caacttcacg cagttgccct
    36541 gcccagtcga aaatatcctg gtacagctgg cgatggatag tgcgcaaatg cggtaaaccg
    36601 cgcaccagcg gaccaagctc aatggtcgca gcacgcagcg ccgtcatttc gtaagcggcc
    36661 tgttccagcc gctgctgctg gcggatgttc agccggttac gcatgatatc aaggccagga
    36721 taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgcctcc tcagctcttc
    36781 aaggcgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga cgccttcaag
    36841 acggcgactg gcctggaaat tacgattacg ctgaagctcc cagagacggg acttttgctt
    36901 atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac aagtataagc
    36961 ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg cgcgagaaat
    37021 cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac attctggtac
    37081 ggaccaacat catgagtcgg cacctgggaa atcttatcgg caacgtccat gcctttcacc
    37141 actttaccaa ataccgcgta accgaaatca cgctgaccat ggtcaaggaa ggcgttatcg
    37201 gcaacgttga taaagaactg gctggtggcg ctgtctttgt cagcggtacg cgccatcgcg
    37261 atggtgccac gcgtgttgcg caggccgtta tcggcttcat ttttgattgg cgggtttggt
    37321 tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc aggaatgacg
    37381 cggtgaaagg tagtgttgtt ataaaaaccg ctgttcacat aatcgacaaa gttttgcaca
    37441 gacactggcg ctttttgttt atccagctcc agttcgatgt taccagctga ggttgtcaac
    37501 aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga aagagcagaa
    37561 agagcgaaaa cagcagccat cgccgccagg gtcgatttga acatgagatt tccttaacaa
    37621 acagagcaga aaaaaagcaa gtgaagagat tctaaatagc cttcaagaac aaggccatcc
    37681 ctttacctaa ttttacgtat ctgaaatata tgtaacctga cgatcacttt ttattccgtg
    37741 atcaaaatca cctcttaaaa tgcaatttag caaccgattg caataaaaca tttaaacaga
    37801 tcataaaatc acctaaaatc gcccgctcgc ggcatatccc gctatgccat tttatttttg
    37861 aacgcaggcc aatcatgact aacagcaatc gcatcaagct cacatggatt agctttctct
    37921 cctacgcact gactggtgcg ttggttattg tcaccgggat ggtgatggga aatatcgccg
    37981 attatttcaa tctgcctgtt tccagtatga gtaacacctt caccttcctc aacgccggca
    38041 ttttaatctc tatcttcctc aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt
    38101 tacgttttgg ctttctcctg atggtgctgg cggttgccgg tttgatgttc agccacagcc
    38161 tggcgctgtt ctcgacggcg atgttcattc tcggggtggt cagcggcatt actatgtcga
    38221 ttggtacatt cctgataaca caaatgtatg aagggcgtca gcgcggttcc cgcctgttat
    38281 ttactgactc cttcttcagt atggctggga tgattttccc aatgatcgcc gcgtttctac
    38341 tggcgcgcag cattgagtgg tactgggttt atgcctgcat cgggctggtg tatgtcgcta
    38401 tttttattct gaccttcggc tgtgagttcc cggcactggg caaacgtgca ccaaaaacgg
    38461 atgctccggt agagaaagaa aagtggggga tcggcgtact gtttctctct gttgcggcgc
    38521 tgtgctacat cctcggtcag ttaggtttta tctcctgggt gcctgagtat gccaaaggcc
    38581 tgggcatgag cctgaacgac gcgggcacgc tggtgagtaa cttctggatg tcatacatgg
    38641 tcggcatgtg ggcgttcagc tttattcttc gcttctttga tttgcaacgc attctgaccg
    38701 tactggctgg tctggctgcg attctgatgt acgtctttaa caccggaaca ccagtacata
    38761 tggcgtggtc aattctcgct ctgggcttct tctccagcgc gatctatacc accatcatca
    38821 ccctgggttc acagcagacc aaagtaccgt cgccaaaact ggttaacttt gtcctgacct
    38881 gcggaaccat cggtactatg ttgacctttg tggttaccgg cccgattgtt gaacatagcg
    38941 gtccgcaggc ggcactgctt acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct
    39001 tcctgttagg tttcgtcagc cgtcaccgtc agcataacac cctgacctct cattaattgc
    39061 taatgccgga cggcactatc gtcgtccggc cttttcctct cttcccccgc tacgtgcatc
    39121 tatttctata aacccgttca ttttgtccat tttttgcaca aacatgaaat gtcagacaat
    39181 tccgtgactt aagaaaattt atacaaatca gcaatatacc ccttaaggag tatataaagg
    39241 tgaatttgat ttacatcaat aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa
    39301 aagaaatcga ggcaaaaatg agcaaagtca gactcgcaat tatcggtaac ggtatggtcg
    39361 gccatcgctt tatcgaagat cttcttgata aatctgatgc ggccaacttt gatattaccg
    39421 ttttctgtga agaaccgcgc atcgcttatg accgcgtaca cctctcgtct tacttctctc
    39481 accacaccgc cgaagagctg tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca
    39541 aagttctggt cggcgaacgc gctatcacca tcaaccgtca ggagaaggtg attcactcca
    39601 gcgccggacg taccgttttt tatgacaagc tgatcatggc aaccggttcc tacccgtgga
    39661 tcccaccaat caaaggttct gatactcagg actgctttgt ctatcgcacc attgaagacc
    39721 tcaacgccat tgaatcctgc gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc
    39781 tgttaggtct ggaagccgct ggcgcgctga aaaacttagg tattgaaacc cacgttatcg
    39841 aatttgcccc tatgctgatg gcggaacagc ttgatcagat gggtggcgag cagctgcgtc
    39901 gcaaaatcga aagtatgggc gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc
    39961 aggaaggtgt cgaagcgcgt aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg
    40021 actttatcgt cttctctacc ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc
    40081 tggacgttgc tccgcgtggg gggattgtca ttaatgattc ctgccagact tccgatccgg
    40141 atatctacgc catcggtgaa tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac
    40201 caggctacaa aatggcgcag gtcgccgttg accatattct gggtagcgaa aacgcctttg
    40261 aaggtgctga ccttagcgcc aagctgaaac tgctgggcgt agacgtaggt ggtattggtg
    40321 atgcgcacgg tcgcacgcct ggcgcacgta gctatgttta cctcgacgaa agtaaagaga
    40381 tctacaaacg cctgattgtc agcgaagaca acaaaaccct gctcggtgcg gtactggtgg
    40441 gcgataccag cgactacggc aacctgctgc aactggtgct gaacgctatc gagctgccgg
    40501 aaaacccgga ttctctgatt ctgccggcgc actcgggtag cggtaagccg tctatcggtg
    40561 ttgataaact gccggacagc gcgcaaatct gctcctgctt cgacgtcact aaaggtgatc
    40621 tgattgctgc catcaacaaa ggctgccaca cagttgcggc gctgaaagct gaaaccaaag
    40681 cgggtactgg ctgcggtggc tgtatcccgc tggtcactca ggtactgaac gcggaactgg
    40741 cgaaacaggg catcgaagtt aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg
    40801 aactgttcca tttgatccgc gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac
    40861 acggcaaagg ctacggttgt gaagtttgta aaccaaccgt cggttcgctg ctggcctcct
    40921 gctggaacga atacattctg aagccggaac atactccgct gcaggattct aacgacaact
    40981 tcctcgctaa catccagaaa gacggcacct actcggtgat cccgcgttct ccgggcggtg
    41041 aaatcacccc ggaagggctg atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca
    41101 ccaaaatcac cggctcccag cgcctggcga tgtttggcgc acagaaagac gatctgccgg
    41161 aaatctggcg tcagctgatt gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac
    41221 tgcgtatggc gaaaacctgc gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg
    41281 tcggcctcgg cgtggaactg gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga
    41341 agttcggtgt ctccggctgt acccgtgaat gttcagaagc tcagggtaaa gacgtgggta
    41401 ttatcgccac tgaaaaaggc tggaacctgt atgtttgcgg taacggcggc atgaaaccgc
    41461 gtcatgcgga tctgctggca gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc
    41521 gcttcatgat gttctacatc cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag
    41581 aaaacctcga aggcggcatc gattacctga aagcggtgat cattgacgac aaactggggc
    41641 tgaacgctca tctggaagaa gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga
    41701 ctgaaacggt caatacaccg tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca
    41761 agcgtgaccc gaacgtgcag atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt
    41821 atgaacgtat cccggtaact ctggtggagg acaacgcatg agccagtgga aagacatctg
    41881 caaaatcgat gacatcctgc ctgaaaccgg cgtctgcgca ctgttaggtg acaagcaggt
    41941 agcgattttc cgcccgtatc acagcgacca ggtgtttgcg atcagcaaca ttgatccgtt
    42001 cttcgagtcc agcgtgctat cacgcgggct gattgcggaa caccagggcg agctgtgggt
    42061 cgccagcccg ctgaaaaaac agcgttttcg cttaagtgat ggcttgtgca tggaagacga
    42121 acagttttcc gtcaaacatt acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg
    42181 ttaatgtttt aacgggaggc gcaatgcctc ccctttttgc atggtcctgt aatgaccttc
    42241 ggtatattgc aggacatttt ttaaactttt tgttttattt tttgttttta ttttttaaag
    42301 gataatcaaa tgtttacaga cactattaat aagtgtgcgg ctaatgctgc gcgcattgca
    42361 cgcctgtcgg caaataaccc gctcggcttt tgggtcagct ccgccatggc gggcgcatat
    42421 gtgggtcttg ggatcatcct gattttcacg ctcggtaatt tgctcgaccc gtccgtacgt
    42481 ccgctggtga tgggcgcgac ctttggtatc gccttaacgc tggtgattat cgccggttct
    42541 gaactgttca ccggacacac catgttcctc acctttgggg taaaagcggg cagcatcagc
    42601 cacgggcaaa tgtgggcaat cctgccgcaa acctggctgg gtaacctggt cggttccgtc
    42661 ttcgttgcca tgctctatag ctggggcggc ggtagcctgc tgccggtaga taccagcatc
    42721 gttcactccg tcgcgctggc taaaaccact gcaccggcaa tggtactgtt cttcaaaggt
    42781 gcattgtgta actggctggt ttgcctagca atctggatgg cgctgcgcac tgaaggggcg
    42841 gcgaaattta tcgctatctg gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac
    42901 tctatcgcta acatgacgct gttcgcgctc tcctggttcg gcaaccacag cgaagcctac
    42961 acgctggcgg gtattggtca taacctgctg tgggtgacgc tgggtaatac tttatcaggt
    43021 gccgtattca tgggattggg ttattggtat gctacgccga aagcgaatcg tccggttgcg
    43081 gacaaattta atcaaactga aacagctgcc ggttaattac taaggggttt ttacgtggat
    43141 catttgccta tattttgcca attacgcgat cgcgactgtc tgattgtcgg cggtggtgat
    43201 gtcgcggaac gcaaagcaag gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca
    43261 ttagcgttta ttccacagtt caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa
    43321 gggccatttg atgaaagcct tctcgacacc tgctggctgg cgattgcagc gacggatgat
    43381 gacacgctta accagcgcgt cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta
    43441 gttgatgcgc cgaaagccgc cagctttatt atgccgtcga ttattgaccg ctcaccgcta
    43501 atggtagcgg tctcctctgg cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa
    43561 ctcgaatcac tgctgccgtt gcatctgggc caggtggcga aatatgccgg gcaattacgc
    43621 gggcgagtga aacaacagtt cgccacgatg agtgagcgtc gccgtttctg ggagaaattg
    43681 ttcgttaacg accgcctggc gcagtcgctg gcaaacaacg atcagaaagc cattactgaa
    43741 acgaccgaac agttaatcaa cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt
    43801 gcaggtccgg gcgatgccgg gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca
    43861 gatgtggtgg tctacgaccg tctggtttct gacgatatta tgaatctggt acgccgcgat
    43921 gctgatcgcg ttttcgtcgg caaacgcgcg ggataccact gtgtaccgca ggaagagatt
    43981 aaccagatcc tgctgcggga agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc
    44041 gatccgttta tttttggccg tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt
    44101 ccgttctcgg tggttccggg tattaccgca gcttctggtt gctctgccta ttcgggtatt
    44161 ccactcacgc atcgcgatta tgcccagagc gtacgcttaa ttaccggaca cttaaaaacc
    44221 ggtggcgagc tggactggga aaacctggcg gcagaaaaac agacgctggt gttctatatg
    44281 gggttgaatc aggccgcgac tattcagcaa aagctgattg aacacggtat gcctggcgaa
    44341 atgccggtgg caattgtcga aaacggaacg gcagtcacgc agcgcgtgat tgacggtacg
    44401 ctcacgcaac tcggtgaact tgctcagcaa atgaacagtc catcgctaat tattattggt
    44461 cgggttgttg gcctgcgcga taaactgaac tggttctcca atcattaatt taacccgggc
    44521 cagagaattc tggccttctt aatgactctt tttatatatt cagcaaataa aacataaccc
    44581 cttaataaat attatctgct aatgggttat attcaccgtt acagcgactt acaaaattaa
    44641 gtcattcgct cactgacgcc aattaaatat tcctgtatcg ttcttaacaa gcagtctgct
    44701 tatattcata taatcaatga atattaatta atatataata catagggaat gtaaatgaac
    44761 aaatttatta aagttgcact ggtaggtgca gtactggcta cgttaactgc atgtactggt
    44821 catattgaaa accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct
    44881 atttctaaaa tcattggcgg ttgcggtcct actgcacagt aagtctctga cagaaatcgg
    44941 ctaacaccgg tttaattgcc cgacgccctc tggattttcc gagggcgtat ttgtttctat
    45001 gtccttcttg cccccgcccg taaaaatatt ttcactttaa attcaatttt ataactacat
    45061 ccattagcca cagcgcgatc ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca
    45121 taatatgttg ttatattcat catgcattgt catgttacct tttaaatgac tgcaaactct
    45181 cccctacaac gtattggaca agaaaaaggt atcgctatgg gaagccaaga actccaacgc
    45241 aagctcggat tttgggccgt tcttgcaatc gccgtcggga caaccgtcgg ctccggtatt
    45301 tttgtatctg tgggtgaagt ggcaaaagca gcgggcacgc cgtggcttac ggtgctcgcg
    45361 tttgtcattg gcgggttaat tgtgatcccg caaatgtgcg tctatgcgga actatccacc
    45421 gcttatccgg aaaatggcgc agattatgtt tatctgaaaa atgccggaag ccgaccgctg
    45481 gctttcctct ccggctgggc cagcttctgg gccaacgatg cgccgtcatt gtcgattatg
    45541 gcgctggcga ttgtcagcaa tcttggcttt ttaacgccta tcgatccgtt gctcggtaaa
    45601 tttatcgccg ccggattaat tatcgccttt atgttgctac acctgcgctc cgttgaaggc
    45661 ggcgcagcgt ttcagacgct aattaccatc gccaaaatta tcccgttcac tatcgtcatt
    45721 ggccttggga tcttctggtt taaagcggag aattttgccg cccttaccac cactgcgatt
    45781 ggcgcaacgg gcagctttat ggcgctgctg gcggggatct ctgccaccag ttggtcgtat
    45841 accggcatgg cctctatctg ttatatgacc ggcgaaatta aaaaccccgg aaaaaccatg
    45901 ccacgagcgc tgattggttc ctgtctgctg gttctggtgc tctacaccct gctggcgctg
    45961 gtgatttccg gcctgatgcc cttcgacaaa ctcgccaatt ctgaaacgcc gatttccgac
    46021 gccctgacct agatccccgc actcggcagc accgctgggc tctttgttgc catcacggcg
    46081 atgatcgtca ttcttggttc gctttccagc tgcgtgatgt accagccgcg gctggaatac
    46141 gcgatggcga aagacaacct gttctttaaa tgcttcggcc atgtgcatcc gaaatacaac
    46201 acgccggatg tctccatcat cctgcaaggg gcgctgggga tcttcttcat cttcgtttcc
    46261 gatctcacca gcctgctggg ttatttcacc ctggtgatgt gtttcaaaaa taccctcacc
    46321 ttcggctcca tcatctggtg tcgtaaacgc gacgattaca aaccgctgtg gcgtactccg
    46381 gctttcgggc tgatgaccac cctcgccatt gcgtcaagcc tcattctggt cgcctcaacc
    46441 tttgtctggg caccgattcc cggccttatc tgcgccgtca tcgttattgc tactggtctg
    46501 cctgcttacg ccttctgggc gaagcgtagc cgccagctca acgctttgtc gtaatgttat
    46561 ctgaagaaaa taaaatgttg gatattgata aaagcaccgt ggactttctg gtcaccgaaa
    46621 atatggttca ggaagtggaa aaagttctca gccatgacgt tccgctggtg cacgccatcg
    46681 tggaagagat ggtgaagcgc gacattgatc gtatttattt cgttgcctgc ggctcgccac
    46741 tcaacgcggc gcaaacggcg aaacatctgg cggatcgctt ttccgatctt caggtctacg
    46801 ccatttccgg ctgggagttc tgcgataaca ccccgtatcg cctcgacgat cgttgcgcgg
    46861 tgattggcgt ttctgactac ggtaaaacgg aagaggtaat caaagcgctg gagctgggcc
    46921 gggcctgcgg cgcactcact gcggcgttca ccaaacgcgc ggacagcccg attacctcgg
    46981 cggcggaatt tagcattgat tatcaggccg actgtatctg ggaaattcac ctgctgctct
    47041 gctacagcgt ggtgctggag atgatcaccc gcctcgcgcc gaacgcggaa atcggcaaga
    47101 tcaaaaacga tctcaagcag ttgccgaatg cgctcggtca tctggtacgc acctgggaag
    47161 aaaaaggccg ccagcttggt gaactggcca gccagtggcc gatgatttat accgttgctg
    47221 cgggtccgct gcgtccgctg ggttacaaag aaggcattgt aacgctgatg gaatttacct
    47281 ggacgcacgg ctgcgtgatt gagagcggag agttccgcca tggcccgctg gagattgtcg
    47341 aaccgggcgt tccgttcctg ttcctgctcg gcaatgatga aagtcgccac accaccgaac
    47401 gcgccattaa ctttgttaaa cagcgtactg acaacgtgat cgtcatcgat tacgccgaaa
    47461 tttcgcaagg gctgcacccg tggctggcac cgttcctgat gttcgtgcca atggagtggc
    47521 tctgctacta cctgtctatt tacaaagatc acaacccgga tgaacgccgc tattacggtg
    47581 gtctggtgga atattaatcc ctctcccggc ccggcaacgg gccggattta cgcaaggagt
    47641 tacccgatga aaacaggtat gtttacctgc ggccaccagc ggctgcctat tgaacacgca
    47701 tttcgtgatg caagcgagct gggttacgac ggcatcgaaa tttggggcgg tcgcccgcac
    47761 gcgttcgcgc cggacttaaa agcgggcggc atcaaacaaa tcaaggcgct ggcgcagacg
    47821 tatcagatgc cgattatcgg ttacacgcca gaaaccaacg gctatccgta taacatgatg
    47881 ctgggcgatg aacatatgcg tcgtgaaagc ctggacatga tcaagctggc gatagatatg
    47941 gcaaaagaga tgaacgcggg ttatacgctg atttctgcgg cccacgcggg ctatctcacg
    48001 ccgcctaacg ttatctgggg gcggctggca gagaacttaa gcgaactgtg tgagtacgcg
    48061 gaaaacatcg gcatggatct gatcctcgaa ccgttaacgc cgtatgaatc gaacgtcgtg
    48121 tgtaatgcta atgatgtgct tcatgcgctg gcgctggtgc cttcgccgcg cctgttcagc
    48181 atggtcgaca tttgcgcgcc gtatgtccag gcggaaccgg tgatgagtta tttcgacaaa
    48241 ctgggcgata aattacgtca tctgcatatt gtcgacagcg acggggccag cgacacgcat
    48301 tacattcctg gagaaggcaa aatgccgctg cgggaactga tgcgcgatat tattgatcgg
    48361 ggctatgagg gttactgtac ggtggagctg gtgacgatgt atatgaacga gcccagactc
    48421 tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc cggaggatga gagatgaaaa
    48481 ccctggcgac aatcggcgat aactgcgtcg atatctaccc gcaactgaat aaagcgtttt
    48541 ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg ctacggcata cagccgggat
    48601 gcattacctg ggtgggtgac gatgactacg gcacaaagct gaagcaggat ctcgcccgca
    48661 tgggcgtcga tatcagccat gtccatacga aacacggcgt taccgcacaa actcaggtgg
    48721 aactgcacga caatgatcgc gtttttggcg actacaccga aggcgtgatg gccgactttg
    48781 ccctgagtga agaggattac gcctggctgg cgcagtatga cattgtgcac gcggcaatct
    48841 ggggacatgc ggaagacgca ttcccacagc tgcacgctgc gggcaaactt accgctttcg
    48901 acttctccga caagtgggac agcccgctct ggcagacact ggtgccgcat ctcgattttg
    48961 cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct gaagatgaaa gcgattgttg
    49021 cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa cggcagcatt gcctgggatg
    49081 gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt tatcgacacc atgggtgccg
    49141 gagattcgtt cattgccgga ttcctttgcg gctggtctgc ggggatgaca ttaccgcagg
    49201 cgatggcgca gggaacggcg tgcgcggcga aaaccattca gtaccacggt gcctggtagg
    49261 tataacgttg gcgtgagcat cttcacgcca acgtgctgtt acttgccgga aaacgaccct
    49321 ataatccgag taattcattc tttatttcag ggtcgattat gtcagctacg gaccgctact
    49381 ctcatcaact cctctacgct accgtccgcc agcggctgct ggatgatatc gcgcaggggg
    49441 tttaccaggc cgggcaacag atccctaccg aaaacgagct ttgtacacaa tataacgtca
    49501 gccgcattac cattcgcaaa gccatcagcg acttagtggc agacggcgta ctgatccgct
    49561 ggcagggaaa aggcaccttt gtacaaagcc agaaagttga aaacgccctg cttactgtca
    49621 gtggttttac cgattttggc gtctcacaag gcaagtcgac gaaagagaaa gtgatcgaac
    49681 aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa catccccgga aacagcgaag
    49741 tgttccatct ctgccgggtg atgtatctcg ataaagagcc gctgtttatt gatagttcat
    49801 ggatcccgct gtcgcgttat cctgactttg atgagattta cgtcgaagga agctccacct
    49861 atcagttatt tcaggagcgt tttgacacgc gagtggtcag cgacaaaaag accatcgata
    49921 tctttgccgc cacccgcccg caggcaaaat ggctgaaatg cgaactgggc gaaccgttgt
    49981 ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc ggtgcacgtc tccgaactct
    50041 tctgccgcgc caatcgcatc accttaacga ttgataataa aagacattaa ccgtaggccg
    50101 gataagatgc gccagcatcg catccggcga tgctggcgca ctgaatttta catcccgcac
    50161 gttcccctca ccctaaccct ctccccaaag gggcgagggg accgttcagt gcggaattta
    50221 gcttgattgc cgaacggtgc tatccagcat taaatactcg caatactctc acgcaaaatc
    50281 cgcagcaccg tctcttcacc gctgcgattc gggttaatgc gaatcgcaca atgttcgagt
    50341 tgtggattcg cctggcgaaa cgttcctgaa agtcgataga agagcggcgg gatttcatat
    50401 ttcgactctg caccgaccgg gtaaggcaac gcgccacgct tttgcgcttc ttccagcacc
    50461 ctggcggcaa tcagctgatg aaactcgaca atcaacacct tcgactgcgc attggcaatg
    50521 acggcgcttt tcacttccgc caccgcgccg ccgttaagca aagccagcaa ccgttcggat
    50581 actcccgcct gcaccgcatg catcactggc gcgaacacca gaccacgcaa gacttccagc
    50641 gcctgtgagc cctggatctg gctaccgccg gagtaaagcg tggcgcgaat ccgactaata
    50701 acatcggcat cgccaaccac tgcaccaacg ccccccggcc caaacagctt gaagcaggag
    50761 aatgtcgaga cattcgcgcc gcattcacag ccgattcgcg ctaccttcat caccgcatag
    50821 ttatcatcgg ttaacgctgg aacacctgcc gcgcgcaacg ttgccagcac atctgccagc
    50881 acatagctgt cctgcggctg ctggcgcgta tgctgcacca gcgcggcatc aggttgttgc
    50941 tcgtcgacga cctgcttcag tgccgacagg tcattgaaat cagcagtaat aagcgtcagc
    51001 cccatctgct caataataac ccgtgtcgtc gggtaaacag gcgcgtcatg caccagaaga
    51061 cgctgccccg gtttgagcaa agccgccagc ccggcgcgaa tcgcgccagt ccccgcgccc
    51121 tgcaccagcg ccgcggcctg tgcgtgaaat gcatcagcca gcacctgctc cacccgttgg
    51181 gtaatacgcg gttgattcag acttggagtt aaccctaaat caccgccagc aagaaactcg
    51241 ctgcctggaa aatggcggca aatagtgtcc accagcgcaa actgcttttg ctgcgcttca
    51301 gcaagagtca ggctttgcaa aggaaacgtc ttcataggtg ccttccttaa gccggaacga
    51361 acaggccaag ccagtaaaga acattcagca gaataccggt gatcattact gccacgaccg
    51421 gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa atggcaaccg
    51481 cgatagagaa tccggtataa cccgccatct taattgctgc gaaaatcgaa ccgaccagca
    51541 gcgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca ttacgcaccg
    51601 acgggtagcg tcccagccat ttgccgatcg aacgaagcag caagacttcc gccgaaataa
    51661 ccactgcgcc taataccgct gcaaccatcg gattcggcga gagatagccc accgcataaa
    51721 caaaggtaaa gcccgcaact gcatacacac ccgttgctaa ggcggtggtg gcaatcaacg
    51781 gcacaaaccc cagtccgcgc ataaattctg ccagagccgc ctgattaatc agcgtttgcg
    51841 attgttccgg cgtcacgcct gcggaatacg ctttctccag tgtgaagatc gaaacttcgc
    51901 taccagcaaa aatcttcata ctggcaacgg cggcaatcaa tgctcccacg atggcgatat
    51961 aaggtaagtt tttgataatc cgcgacgtgc gctcttcaaa caccgaaagc ccgctggcat
    52021 cgatgtcatt ttcatcacga tgacgcaggt cgtgagttat cgcgatcccc agcagcatca
    52081 ccatgccaat aaagatttcg atggattcag ggttaagatg tgggaaatag cgcacgacta
    52141 ccacacgggt catcagaaca accacggcgg cgaccagact ttgcttccag ccaaactggt
    52201 agaaaatcgc caccagcggg aacagtgcaa aagcagaaac caccggcgag cttaattccc
    52261 cgaggctacc taatacatcc accggcagcg cggtcagcag ctggttaact ggcaacaggc
    52321 aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg ctgtttatcg
    52381 ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgttgagc agcccggttt
    52441 tcagggtaaa agagatcccc accgaggcca caaaaccaat gctcagacca aaagcgatac
    52501 tccccgcctc gcgacggttc atatagcctt caatcagttg cggcaggatc gggtggatgc
    52561 cgtcatgaaa aacagccgcc gagcgatgcg ccagaagcga tgtcataccc gtcaggcacg
    52621 ccaccacgat aatctgaata tacagatcca tagtcgtgcc ttttatttca gatggttaat
    52681 caacatcgga atcgcgtgtt cgacgtgctc aacggaaagg ccaaacgcca ctttcccttc
    52741 agcgatcatt ttggcgatat gctcgtcttt cgctttaatg cctggtttgg caatggtgca
    52801 acttttgtta tagccaatca ccgcgatggc aatcgacaat gccgcgcccg caccggtatt
    52861 acacgcgcca atgtaataat ctagttgccc ggatttcacc ttcattgccg cttccatgtc
    52921 gttgtgaatg aaaacttcaa aacagccagg agccgtcgct tcaatggttt ttttaatctg
    52981 ctcacgctgt aagcctgcaa cgccaatctt tttcatgagt ctgtccttat tggaaaaatt
    53041 gagagggatt ttcacgtaac atcacatcca catcggcctg actgaatcct gactggcgca
    53101 attgcggaat aaaggtggtc aataaatagt catagccata accaccattg gcttttaaat
    53161 gggagcggcg cgtaatatcc atcgacagca tgacgcggtt cagcaaccca cggtcacgta
    53221 gcgcgtggag catcgcaatg cgcttttcgt ccgggtagta actgttctta ccaatggtgt
    53281 cgaactgcac gtacgcgccg agatcgatca tcttcaaaat gttgtcgagg ttgtctttca
    53341 gatcgcagtg accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc aacagagcca
    53401 gttgttccag ccccatggtg ctgaacgacg tatgcgtgga gatcgggcgt ccggtctggt
    53461 tatgcgccag cgcagcggca ataaatacct tctcttccag cggcgtaatc tttccttcgc
    53521 tggagccgat ctccgcgatg atcccggctt tcagctccgt gccatcgata ccctgttcaa
    53581 tttcatcgac catctcctgc gccagttcct gcacgctgcg ggtcaccaca tgttccggga
    53641 aaaacgcatc ctggtaataa ccagtacagg ccaccacgtt gatcccggtt tcgcgcatta
    53701 catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca atcacattac
    53761 gtacaccccg ggtcatcagg tcgttcatct cctggcaaat gaacgcatac tgatcaaggc
    53821 ggcagtccac gttgttttta aagccggaga gatcaatatg cagatgctca tgggccaggg
    53881 tgtaacccgt cggatcaaaa ctcatagggt gtctcctgca aaccggaggg aggaaagaaa
    53941 agagcgacca ttttgtggcg gtggcgcacg gaaaaattca cacacggtag ctcccacatc
    54001 agaaagcgtg gtgcgcacac cgagctgcgt ggcgaccagc ccttgctgat aaaccagcac
    54061 tggcaccact tcacgggtat ggtggctgtg accaatggtc ggatcgttac cgtgatccgc
    54121 catcacgacc aggcaatcat ctggctgcat cgcctcaaca agccgggcaa ggttacggtc
    54181 aacgacctgc aaacgttcgg cataacgtgc gacgtcttct gcatgaccag cgaggtcggt
    54241 ttcctggata ttgatgcaaa taaacgccgt cggatgggtg ttaaattcgt cgagggtgat
    54301 atccataatc cgctggctat ccaccaggtt ttgccagctc acaccataag gattgctgac
    54361 gatatctgcc accttaccca ccagcacggt tggcacgccc gcttcataca gtttttgcgg
    54421 cacctgcact ttttcatcaa caccgtagcc catatgcacg acctggaagc cgttgtcata
    54481 agcgccagaa cgcggcgcat taataccaat aaagcgccct tctttgcttt ctgcggcatc
    54541 gagaatacgc tgactgttgg ttaacaggcc gccaaatgta atgacccgac cgacctgtac
    54601 ctggtcacgc acgatacgac caattttgat tgcgtcgtca aaagagatca cagagagatt
    54661 ggcggtaatg ttatagactt ggcctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc
    54721 ctgattgacc gacagaaatt gcagatcatc gccacggcgc tccacctgcc agccagcgga
    54781 aactaatgcc tgctcaacac ggtcaatcac atcgcgaaaa ggcatccgca gcggcggtaa
    54841 cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt catgttgcag
    54901 ctctgccacg ccccaggttg cggaatctga cggctgcata tcgcctggcg cataacccaa
    54961 tgtgttgatt agccccagct tctccagcgt tggtagctgc aaatgcggca actggctcag
    55021 gatgtgacca caggtattcg ccccggcatc ttgcgggcgc accagcgtga catctttcat
    55081 tgcccctacg ccaaagctat caatcactaa caccacaaat cgcgccattt atgcctccag
    55141 agggttgccg aggctgtcat aacgaccaac tatttctggt tcgccgcgct gaataccgga
    55201 caccagcacc acatcgctac gggtgacaaa aatctgcgtg cgaaaacaga gcaccactgc
    55261 actgctcacc ggatactcgc ctgccagcgg cagggtgtag tcgatactgc tgtcatccac
    55321 agttttgaga ttggtttcag taatcttttg attttctggc gtaaacacca gcgcatgttg
    55381 cgcatgacca cgacggtagt aaccgccgcc gtagcagtag ctgtcgccac ggaaatgatg
    55441 ggagatttca cttaaccaga gcatcgcgat acgttcaggc tgatcgccct gctggtttgc
    55501 cggaatagta ccggtcagcg catgaccggg ttcggcatga gtcgcaccgt attccgccag
    55561 caatggcagc gaagtgcagc tggtcgctga aggtacgttc agttgctcaa tcgcaatacc
    55621 agatttcgcc agttgatccc gtgcttgtac cagcgtgtga agattcggtg ttggcaaaac
    55681 tttcccagca gcctcatccc aaagcaggca agggaaatgg gttagtccgg ctaaatgcag
    55741 ccccggcagt ttcttaattt cagcaatcac ctcgtgcagc gaatgctgta caaaaccgct
    55801 ctcctgcccc ggataaagaa aatcatcgtc gctataaact ttgagcagca caggctggac
    55861 ccgtccggcc ttaaccgctg ccgcagaaac ttcccgcgct ttgtcgagag taaacacggt
    55921 gatgatgtcg gtgccctgtt caacggcgtc agcaacctga tgacaaggga tttgtaccag
    55981 atgcccctga tgcgccactg gcaaaccagc gcggcgcatg actcgcgcct ctttgtaatc
    56041 caccgccaca atgccgctgt agcctaatgc cagcaatttt tccgccagcc acggattacg
    56101 accaaattgc ttggtcatca gatacagttc aatgccgtaa agccgcgccg tctcaatgag
    56161 ccgcttaccg ttttccagta tctgatccac gtcgatcacc cagctgtccg gggcgatttt
    56221 gccctgctgc cacaaactta gtgcggcgga aatcagcgcc gggttctggc gtttcaatgc
    56281 ttctacaaac atcttgttgc ctcgtttctg ttccttgaat taaatattca tttttttgaa
    56341 tatttaggtt gtgcgacgtg gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc
    56401 cataatccat aaagattggc cagaaaatag ccttcttcac acgggttaac ttccagagcg
    56461 aattccttca acaactcctg atgcaattgc acaacggctt gccagtggct ggattgcgcc
    56521 agttccgcca gcaactcgtt atccagcggc tctatttctt caccacggcg actgcgcatc
    56581 agtgcactcg ccatatgtgt catcgccatc gttccttgct cactgcgcac cggcagatgg
    56641 cactcttttt ccagtacgtt gacgacctgc atcatgcctt tgcagacgtc cttatcaata
    56701 acgcctgcct cgcaaagcag gttgagtctg gtttccatat cagatgcctt tcgtttaata
    56761 actgggttcc tgttccccac tcaccacccg ttgttgatgg gcaagcaggg cgtgtttatc
    56821 tacaacagca cgcagcaatt gttgcattgg gtaatcatcg actcgcgtca ggaccacggc
    56881 ttcggtggcc tgtaaaaatc gcggatcgtc tgttagcggc gtcgcctcta atcccagcat
    56941 ggtcagttcg ttttccgcca ccacgttcca gatcaccgca tcgacatcgc ctttaacaat
    57001 gcgttgtaaa ctctcgtgat aagagagatc gactcgttcc acatcactat cgccaaaaaa
    57061 gacatcggtc atgattttct gatccgccga acgactatcg agacctactc gcttaacatt
    57121 tccagactca cctttgcggc atatcagttg atgttcacca acatacgtat gcgggccaag
    57181 ctcgagggcg atgcacaaat tgttttggct gagataactt tccgccgcca ggcgggaaac
    57241 tactgccatg tcatagacac cgttaagtaa acactccacg cgaatatccg cgccgcgcat
    57301 atgcgcatag taaaaaggaa tgccatcaaa ctgggctttc aatccgctcg ctaaaccttc
    57361 gtacaagcgg gtatagggca aaggcatcgc acataccacg ttgttgatat ccacatgact
    57421 cagcaatgct ttgttatcca tctcgacaag ataactgcca ttgcgcccac ggcgttcaat
    57481 ccgtatcgct ccgcttgatt ccagcgtttt caacgcggcc tgcgtcaggc caaccgatga
    57541 acggcattca gttgccagct catctatggt tttcagtcga ttaccgcatt tttcacccaa
    57601 caaataacgg gccagtgtcg tgatgacgac gccttctttt ttgataaacg ttcgacgcat
    57661 aatatatttt caataaaatg aatatttata tcttcattaa aatgaagata agtggcgacg
    57721 acgagatgtg gaaacggcga ggcgcttcac attttttcgg gttgttgcga aaagaggata
    57781 aaaaaaccgg gtttccccgg tttcagagtg atgacgaagt gatttattcg acaatgcgag
    57841 caaagggtaa ttcccactgg cgcttgcctg ctcccctcac cctaaccctc tccccaaagg
    57901 ggcgagggga ccgtccgagc gttttgatat tacggcttcg ccacaaaacc aatcgcttcg
    57961 tacaccgctt tcagcgtacg ggaagcgtgt acactggctt tttctgcgcc gtctttcatc
    58021 acctgttgca ggaaggcttc atcgttgcgg aaacggtgat agcgttcctg caattcagtc
    58081 agcataccgg aaacggcatc agccacttca cctttcagat gaccatacat cttgccttcg
    58141 aactgttttt ccagttctgg gatgctctgg cccgttaccg cagaaaggat atccagcagg
    58201 ttggaaacgc ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggagtca
    58261 gtgaccgcgc gtttgatttt cttcactacc gatttcggat cttccagcag gccgataacg
    58321 ttattgcggt tatcgtcaga cttggacatc ttcttggtcg gctccagcag cgacattacg
    58381 cgcgcgccag atttcggaat aaacggctcc ggcaccttaa agatctcgcc atacagcgcg
    58441 ttgaaacgct gggcgatatc gcgactcagt tccaggtgct gtttctggtc ttcacccacc
    58501 ggtaccagat tagtttgata cagcaggatg tccgcagcca tcagcaccgg atagccaaac
    58561 agaccagcgt tgatgttctc ggcataacgc gcagatttat ctttaaactg cgtcatgcga
    58621 ctcagttcgc cgaagtaggt atagcagttc agtgcccagc ctaactgcgc atgttctggt
    58681 acgtgggact gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag
    58741 gccagcgtat ccaacgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg
    58801 tgttggtcaa cgatacagta aacgcaatgg tagtcatcct gcatgtttac ccactgacgc
    58861 agcgcaccca tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg
    58921 atgggcttag tcatttttcg attcctgatt ttcgctatgc ggaagcccta atgcgggcag
    58981 aaggtcattt atagactgat aaattacatc aggctggctg agatcgatag cctcgccgta
    59041 gttgtatccg taggttaagc caactgatgg gcaacctgcc gcttttgccg cctgaatatc
    59101 attccgtgag tcgccgacaa acagcatctg ttgtggggca attcccatcc gctcagccac
    59161 cagtaacagc gggtccggat gcggtttttt gttttgcaca tcatcgccgc cgataaccac
    59221 gctaaagtat ttggcaatat ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt
    59281 gttggtgacc aagcctagcg gcaggccttt agcctgcaac gcgcccaacg tatcggcaac
    59341 gtgcgggaac aaaaacgtcc cctcttcggc aacctcgcca tagtagcgat cgaacagttt
    59401 acgcagaata cgtacctgtt cttctgccgg aatgtcgtca tcaacgggcg gtttccccat
    59461 tgttttacgc agagtcgcac gttcctgacg cgcccaggtc aatgcgcgct ccatcagaac
    59521 atctgcgccg ttaccaatcc aggtaataac gcgttcttca cctgcgacgg gcaactccag
    59581 cgcatacagc gccatatcta ccgcagcagc aagaccagga gcactgtcga ccagcgtacc
    59641 atcaagatca aaagcgacgc cgcgaatatc ttcaaactta ttcatgactt acctttgcca
    59701 gttcactgcg catttcatca atgacttttt tatagtctgg ctggtcgaag attgccgaac
    59761 cggcgacgaa catatccgcg cccgccgcag cgatttcgcc aatgttgttc accttcacgc
    59821 caccgtccac ttccagacga atgtcaaagc cagactcgtc gatacggcga cgtacttcgc
    59881 gcagtttatc cagtgtttga ggaatgaaag actgaccgcc gaaaccaggg ttgacggaca
    59941 tcagcaggat cacatccagc ttatccatca cgtaatccag atagctcaga ggtgtcgccg
    60001 ggttaaatac cagacccgct ttacagccat tttctttaat cagttgcagc gtgcggtcaa
    60061 catgctcgga ggcttctgga tgaaaggtaa tgatgctggc accagcggca gcgaaatcag
    60121 gcacaatgcg atcgacgggt ttcaccatca gatgtacgtc gataggggcg gtaatgccgt
    60181 agttacgcaa ggatttcagc accattggcc caatcgtcag attgggaaca tagtggttat
    60241 ccatgacgtc aaaatgcacg acatcagcgc cagctgccag ggcttttgcg gtatcttcac
    60301 ccaggcgggc aaaatcagcc gacagaattg agggggcaat caaatactgt ttcatccgct
    60361 tctccttgag aattattttt tcgcgggtga aacgactcct ggtttgtaca aagccagcag
    60421 ttcgtccacc tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac
    60481 atgcaatttt gcgcgctgat accactcacg cgttaacatc gtatcgtgat tggagatcag
    60541 cactggaata tggcgctcaa ccagaccttc ggcgatctcc gccagatgcg cttgttgttc
    60601 aagcgtaaaa ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca gcggtgcata
    60661 aggcggatcg caatagacga cggatgcatc atctgcgcgc tccatgctat cggcgtaaga
    60721 ctcacaatag aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg
    60781 gaaatagggt tttttgtagc ggccaaacgg cacgttaaac tcaccgcgca gattgtaacg
    60841 acacaggccg ttgtaaccgt agcggttcaa atataaaaac agtaccgccc gacggaacgg
    60901 atcctggctt ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc
    60961 gggaacaaac agctcgcgtg cggcctgtac gtactcatca gtacgcatct tcacaatgtt
    61021 atagagactg atcaggtcgc tattgatatc ggcaaggata taacgagaaa agtcggtgtt
    61081 gagaaacacc gacccggcac ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg
    61141 ccgtttaata tcatcaagca ggggatactt gccccctgcc cacttcaaaa aagcgcgatt
    61201 tttcttcatg ccgattaact aattacacct tctccggctg tggagaaagc tccgacagca
    61261 tccagcgctt caggcagctc ccgcaacgat aaaccattgc gggagataac cttgattact
    61321 tcagatcggc ctgtacctga cgcagcggtt tcgcccacgg gtttttggcc tgaacatctg
    61381 ctggcaatgt agataccgct tttttcgcct cttctttcga agcgtacacg ccagaaacca
    61441 ggacatacca cggctgacca ttacgcgtcg tttcatagac aacgtagttt ttcagattct
    61501 ctttcttcgc ccaaccgttc aggttgtcgt agttagagga actgctcagc tgcagagtgt
    61561 aatggctgga cggtgccgat ttcaacgaac caacattacc tgcggtcttc cctccagcgg
    61621 ctggtgttgc cgtggtttgc gccggggatg ccgtctgtac tggagccgta gtcgccgtct
    61681 cttttggtgc tggcgtagaa gtcgcagccg gtgccttcgt gctcgccaca ggggcagctg
    61741 gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg
    61801 gttttttcgg ttcaatcacc gcctgctgac gaactgggcg cgtagtggac ggacgttcag
    61861 cggtttgcgt tttcgccgag tcacgcgatg cattgccatt gcgaacaggc gcaaccgttg
    61921 cgggttcagt cggcaacgtg gaattgaccg ccacattgtt cagctgttgc tgattttgtg
    61981 gctgggtcag cgcattgttc aggtcaccct gcacttcaac acgttgttga ccatccgttg
    62041 ccaccggggt ttgcccttga gtcggcgtag aagagatcgg tggcagcgaa acatcctgct
    62101 gagtattttc cgcagacgtg gttcccggcg ctggctgcac gccattcgcc tgatcggtcg
    62161 cattgccagc aagatcaata ctcttctcgc cagacgcggt ttgatcgctg gaagtggtcg
    62221 agggggcttt tagcgcagaa ccgataccga tgatcaacag cagtagaacc agaatgccga
    62281 cgcccatcat catatactga cgagaagcgg gtttactggc tgcttttttg cgcttacgcg
    62341 gacgacgctc tacgcgctct tcatccacgg tttcatcttc ggattcgtca atttcttctt
    62401 cgatttccgg ttcctcattg cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt
    62461 catcaagttc aatatcatca aaattgatct gcggttcgcc acgttcagta cgctcagaac
    62521 gttcagaaga ttgacgagaa cgaccagtac gacgatcgct gggatcgggt ttcagctcgt
    62581 cttctggttt gaattcatcc atttaacacc ccactaaaag gttaatgctt accacgttgc
    62641 aattaacctg aagctaatag accgcttgat aagcggcctg acctttcttg ttgttacgct
    62701 gattgacaat cggcaatggc gttaagaaca agctcgtgcg aaacgccgct gcgaacttca
    62761 ctcttaccaa ttgccaacgg aagaattaag cgcatctctc ccgcaaggac tttcttgtca
    62821 cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac
    62881 ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga
    62941 cgttccgacg tccgcgccgc catcaccata cccgcagcga ccgcttcacc atgtaaccaa
    63001 ttgccatacc ccatttcagc ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa
    63061 gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt ctgccttcag ttcacaacaa
    63121 cggcgaatac agtacgccat tgccggaccg tccagacgca acaacgcatc cagattctct
    63181 tccagccagt taaaaaacgc accgtcaaga ataatgccgt atttgatgac ttctgccagc
    63241 cccgacgcta actcacgcgg gggaagcgtt ttcagacagt cgagatccac caccactgaa
    63301 gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg gatggttgac cgcagttttg
    63361 ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca
    63421 ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc
    63481 gccaccagcg tagtatcgcg accatgcggc ttttgtaaca acgccgtaaa gacggtatcg
    63541 agtacagcta ggcttttata ctgctcgccg tcagggagga taacgctatc gacgttaaca
    63601 cccgcctgtt caagtacgcc gcggacctta tcgagataca gaggagccag ggtttcgctg
    63661 gtgaccaaca tgacctgctc gcccgatttc agcggtaaga atgaagctgg ttcattaaac
    63721 aaaccagatg cgatggtaat tgggtaacta cgttccccga gagtaacgac aatcctctcc
    63781 ataacgcgac atccacctta attactgtac ccgcagacga gtgtatataa agccagaatt
    63841 agttgctttc cagcatgtga ataatctggt ttgcaaccac tttagcgctt tgatcatcag
    63901 tacgaatggt cacgtcggca atctcttcat acagcggatt gcgttcattg gccaacgctt
    63961 ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg
    64021 tgcgtgcaag ttgcttttcg atggtcgttt caagataaac gacaacgcca cgagcggaaa
    64081 gacggttacg cgtttcacgg gatttcacag agccgccgcc agtagccagc acaataccct
    64141 gtttctcggt caactcattg atgacctttt cttcgcgatc gcggaagcct tcttcgcctt
    64201 ctaaatcgaa aacccagccc acatcagctc cggttcgttt ctcaatctct tgatcggaat
    64261 cgtaaaattc catattgagc tgttgagcta actggcgccc aatagtgctt tttccggcac
    64321 ccataggccc aaccagaaag atattgcgtt tctctgccat tttttcggta ctactaagac
    64381 tattcgttaa tgataaaccc gcttcgctca gagagcgccg caggacatga actgaaacct
    64441 cataagatat tgcgagagtc agactgaaaa ttatctcaat actccagcgg gtttggcaac
    64501 tgaataaatc acccatccct ctgcatatct ggtcgctgca agcgcgctgc cttgctacca
    64561 ccgctctggc gataaatcac cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct
    64621 gaatgataaa tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta
    64681 ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcataaaaac
    64741 attaccgctt ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact
    64801 aactcgcgtc gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg
    64861 tcgccaagca acggtacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc
    64921 agcgccaacg tttctccgct ttttacctcg acctgcgttt cgatctcctg cttatcaatc
    64981 gccagcactt cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt
    65041 aatttcagcc ggatgcgacc tttttgtaac accgtgggcg tgacctccat ccccaggacg
    65101 gcctctttaa attccaccga cgtcgcgcca ctttccccgc tggaaacctg atatggaatt
    65161 tcgctctcct gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactggcg
    65221 ataatatcca gctgctgttt ttgttcgagc gcggaaagct caagatccag caagcgtccg
    65281 ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgcta
    65341 ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg catcggccag cgtccatttc
    65401 acgcctaact cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc
    65461 tgcccgaccg gcagatccat ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg
    65521 ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg tcatactccc tttggcactc
    65581 agtagcttct cccccgcttt cgccagttct cccgcgtcgg cgtattgcag ggttatactg
    65641 cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata
    65701 ttgtcattct gccaggcaat ggaatgcact gagagaatgt tgccttcctg ccgcgttatc
    65761 agtccggcgc ttttcactac agtttgtagt gcctgcttcc agggaacatc tgttagatgt
    65821 aacgacaccg taccgctgac gtctggcgac acgaccaggt tcaacttctc ctgttcagcc
    65881 agcgcctgca acacctgagc taccggaacg tcatccacca tcagcgtcac tttttgcggc
    65941 tttgccgcct gtacgccggg tatcagcatc aacagtagtg cggctatcca ttgcttcatt
    66001 tgtatctcct tgccgttgcc acaaccattg tggcggttcg cagtttgtcc cggtacccag
    66061 cgttagtacg tctggcgtca gctgtaaaat tgtccagccg ttttccagca catcgttttg
    66121 ctgcacccgt cgccatttct tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg
    66181 ccctaccatc ccctgatagc gccactggct aagttcgcta atccggcata gatcttccgg
    66241 cgatttaaaa gggtcacgca taccggttaa aaggcacaat gcaatacctg ccaacaacca
    66301 gcgtttaacc ctcatgcggc atctccagtt gtagcgtgaa caaaagatca tcaccttcca
    66361 cgcttaacga aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg
    66421 gcactgcttc ccacagcgtt ttcaacgcca actcgcctcc ctgcgcggat ggatgccagg
    66481 aaaccagttg cgcgccggat aactgaaaat ccagtggcga aaagggcagc gttttttcct
    66541 cgctgaaggg agtggtgtct accaggcgat acagtgcggc ccactgctgg tgatgacttg
    66601 cccgtagtcg aattaatgcg tcgcgctctt catggtgtgt cgatgataga aaaatgagcg
    66661 taactaacat cagcaaccag aatgcccagc aaaactggcg gaggcggggt gatgtggcga
    66721 accaccagtc aaagaacatg ttcatcgcta accttccttg ttaactgata ctcaaattgc
    66781 cagcgtccct gcgcatcctg ctgcgtggct ccccgctgat tgagatgaaa agacgcatcc
    66841 tggcggagtg acgtttctag tgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc
    66901 tccagcgttc cctgctgcca gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc
    66961 gccagtgctt ccagcgcaga ttgccagtcg cgggtaaatt gtcgctggcg ctgccgttgc
    67021 gaacgctgtt cgcgtaactg ctgttgctcc agcaaacgtg gcttcgttat ctgtaagctg
    67081 cgggcgagtt gttgttccgc ctgaagcaaa actgcgtcta tgcgcgcttc ggcgctgcct
    67141 gtcagacgca gtattagcgt tatcccgacg gccagcagca gaggcgcaac gaacatcagc
    67201 aaccagaaac gcagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc
    67261 gggttcatca gtacgccttc ccaagcgcca gccccagcgc gatggcaaag tctccaccag
    67321 gcggcggtag cggcggctga cgaacagaaa cggcctccca ggggtcaaat ccgccttcgc
    67381 cacatatcgc gacgctgtcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag
    67441 tcatccccac cgccagtttg cgcccccagc tatagcgcgt cgcccacagc cactgttcgt
    67501 tatcacgcca ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg
    67561 cactggcatc cggggtgatc gcactaacat gaacacgcaa cctttctgcc agcgtaagca
    67621 gcgttgccag ctctttgctt tgcgcggcag tcacgttata agcggggctg agtgagtctt
    67681 cgctgtaatc gaagcgcagg gagtccggat ccatatccag ctcgcgggcc atcgtccctg
    67741 acagccaggc cgtttgctcc cgctcaccaa gggacatcga cgggcgcgga aatgaccgct
    67801 gtaatgtgcg actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac
    67861 tccacggtaa caacgtttta gccagctgct gcgcatcaac aatccgccca tctttgataa
    67921 tgtcgtgcgc cagcggcaac cgccaccagc gttgcaaaaa gcattctttt gcgccccgca
    67981 cgatcgcaac cgctgccgct tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa
    68041 atgtcattgt gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg
    68101 cttgccttta tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa
    68161 tttccagtga agttcgtaaa gtattttttg atccttgcag tctgttgcat tctgctggga
    68221 gcaggctcga tttatggcct ataccgctac atcgagccac aactgccgga tgtggtgaca
    68281 ttaaaagatg ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt
    68341 gctcaatacg gtgagaaacg tcgtattccg gttacgttgg atcaaatccc accggagatg
    68401 gtgaaagcct ttatcgcgac ggaagacagc cgcttctacg agcatcacgg agttgacccg
    68461 gtggggatct tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg
    68521 agtaccatta cccagcagct ggcgagaaat ttcttcctca gtccagaacg cacgatgatg
    68581 cgtaagatta aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgaa
    68641 atcctcgagc tttatctgaa caagatttac cttggttacc gcgcctatgg tgtcggtgct
    68701 gcggcacaag tctatttcgg taaaacggtc gaccaactga cgctgaacga aatggcggtg
    68761 atagccgggt tgccgaaagc gccttccacc tttaatccgc tctactcaat ggatcgtgcc
    68821 gtcgcgcggc gtaacgtcgt actgtcgcgg atgctggatg aagggtatat cacccagcaa
    68881 cagttcgatc agacacgcac tgaggcgatt aacgctaatt atcacgcgcc ggagattgct
    68941 ttctctgcgc cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa
    69001 agtgcctatg aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc
    69061 gcgcagcagg cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc
    69121 ccggcaaatg tgctgtggaa agtgggcgag tcggcgtggg ataacaacaa gattaccgat
    69181 acgctgaagg cgctgccgac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat
    69241 cctcagggag cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc
    69301 gttcgctggg cgcgtcctta ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg
    69361 accgatgttc tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg
    69421 ctggcacaag tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaatggtgcc
    69481 gttatggcgc tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag
    69541 gcactgcgtc aggtgggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa
    69601 ggtctgacgc tggcaagtat gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc
    69661 ggttctgact ggcagccgaa gaactcacca ccgcagtatg ctggtccaat tcgcttacgt
    69721 caggggctgg gtcagtcgaa aaacgtggtg atggtacgtg caatgcgggc gatgggcgtc
    69781 gactacgctg cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc
    69841 gaatcgctgg cgctgggttc agcgtccttc accccaatgc aggtggcgcg cggctacgcg
    69901 gtcatggcga acggcggctt cctagtggac ccgtggttta tcagcaaaat tgaaaacgat
    69961 cagggcggcg tgattttcga agcgaaaccg aaagtagcct gcccggaatg cgatattcca
    70021 gtgatttacg gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc
    70081 gctatctccc gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat
    70141 caggcgttag tggcgaagac tggcgcgcag gagtacgcac cgcacgtcat caacactccg
    70201 ctggcattcc tgattaagag tgctttgaac accaatatct ttggtgaacc aggctggcag
    70261 ggtaccggct ggcgtgcagg tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt
    70321 accactaaca gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg
    70381 gtctggattg gctttgatga tcaccgtcgt aatctcggtc atacaacggc ttccggagcg
    70441 attaaagatc agatctcagg ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac
    70501 gcttatatga aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt
    70561 attgtgacgg tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc
    70621 gaagagtatt tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg
    70681 accattatcg ataatggtga ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc
    70741 ttttcagttg gctgacaaag tgcatttgtt tatgccggat gcgggtgaac gcgttatccg
    70801 gctaacaaaa tcgtgaaaaa tcaatatatt gcaggaaccc cataagcctg ataagcgttg
    70861 tgcatcaggc aaacttcacg catttacact cgcccctgcc ctttcaacca ttcgcgcacg
    70921 aggaacagcg cgctgacatt acgcgcttcg ttgaagtcag ggtcttccag caaatccatc
    70981 atatgcgcca gcggccagcg cacctgtggt agcggctctg gctcatcgcc ttccagcgat
    71041 tccgggtaga gatcttgcgc gaccacgata ttcattttgc tggaaaagta agacggtgcc
    71101 atgctgagct tcttcaaaaa agtcagatcg ttcgcaccaa atccaacctc ttcttttagc
    71161 tcgcggttag cggcttcgta gacgctttca cccggatcaa ttaatccttt cgaaaaacct
    71221 aattcgtagg attcagttcc cactgcgtat tcgcggatca ggatcaggtg atcatccaca
    71281 atcggcacaa tcatcactgc ttcccggttg gttggacgca ttcgttcata aacacgccgc
    71341 acgccattgc tgaactccag atccacgctc tcgacggtaa acagtcggga acgggctaca
    71401 gtttcaacat tcagaatggt gggtttttgt aatgatttgt tcatcgtggg atctatgctg
    71461 tgaaatcagc ggttattgtg cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta
    71521 tttacattta tgtaacttaa taaataatcg tcctcaaatc aaattaaaag tcaataggtt
    71581 gaaataactc caggaatttg ctgatattcc gccttcagag ggtttgctat gatcagcggt
    71641 tactgtgatg tgcttaatga tgctcaagtt aagctccacg cttgccgata gccaaccgca
    71701 gaatcatgta ttgtgtccgg tgcgactgac cacgcctgac agactaagta agatggggaa
    71761 agcatgagca ccattgtgat ttttttagct gctttgctgg cctgctcact acttgcggga
    71821 tggctgataa aagtgcgatc cagacggcgt cagctgccct ggaccaacgc cttcgcggat
    71881 gcgcaaacgc gtaaactcac acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc
    71941 ctgacgcagg tattacaggt gcctggccca acgggagcta gcgcggcacc gatctctctg
    72001 gcgctgaatg ccgaaagcaa caacgtcatg atgctgacac acgctatcac acgttacggc
    72061 atctctaccg acgatccgaa taaatggcgt tactacctcg attcggtaga agtccacctg
    72121 ccccccttct gggaacagta catcaacgat gagaataccg ttgaactgat tcataccgat
    72181 tcgctgccgt tggttatttc actcaacggt catacgctgc aggagtacat gcaggaaacg
    72241 cgcggctatg ccttgcaacc tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt
    72301 gagcaaatcg agctactcaa tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt
    72361 ccgcgcgggc tgcgtgaagc gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc
    72421 ctgattaccc cggatgtatt tgttccgtgg ctggcaggcg gcgcattact gctgctgggc
    72481 gcaggtctgt gggggctatt cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt
    72541 ctgcgcggta caccccgtcg ttggggattg tttggcgaaa acgatcagga acagatcaac
    72601 aatatttcac tcggtattat cgacctggtc tatcccgcac actggcagcc atacattgct
    72661 caggatctcg gtcaacaaac cgatattgat atctatctcg accgccacgt agtgcgtcag
    72721 gggcgatatc tttcgctgca tgatgaagta aaaaactttc cgttacagca ctggctgcgc
    72781 agtacgatta tcgctgcggg ctcgctgctg gtgctgttta tgctgttatt ctggatcccg
    72841 ctggatatgc cgctgaaatt cactctctca tggatgaaag gcgcgcagac cattgaagcc
    72901 accagcgtaa aacaactggc tgacgctggc gtgcgggtag gcgatacatt gcgtattagc
    72961 ggtacgggaa tgtgtaatat tcgcacttcc ggaacctgga gcgcgaaaac caattcacct
    73021 tttttaccgt ttgactgctc gcagatcatc tggaatgacg cccgctcatt accattacca
    73081 gaatctgaac tggtcaacaa agcgacggca ttgactgaag cagttaatcg ccagctgcac
    73141 cctaaaccgg aagatgaatc tcgcgtcagt gcctcattgc gttcagcgat tcaaaaatcc
    73201 ggcatggtat tgcttgatga ttttggcgac attgtactga agacagcgga tttatgttct
    73261 gccaaagatg actgtgtgcg actgaaaaat gcgctggtca atctcggcaa cagtaaagac
    73321 tgggacgcgc tggtaaaacg cgccaacgcc gggaagctcg atggcgtgaa tgtgttatta
    73381 cgcccggtga gtgcggaatc gctggataac ctggtggcaa cctccaccgc gccgtttatc
    73441 acgcatgaaa cggcgcgagc ggcacaatca ctaaacagtc cggcccccgg cggattcctg
    73501 attgtcagcg acgaaggcag cgattttgtt gatcagccct ggccttcggc atcactttac
    73561 gactacccgc cgcaagaaca gtggaacgct ttccagaaac tggcacaaat gctgatgcat
    73621 acgccgttta acgccgaagg tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag
    73681 catattggcc ttcatccgat cccggatcgt tccggcctgt ggcgctatct cagcaccaca
    73741 ttgctgctac tgacgatgct gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt
    73801 taccagcgtc atcgcactcg catgatggag attcaggcct attatgaaag ctgcctgaac
    73861 ccgcaactga tcaccccttc agaaagcctt atcgaataac acgtttgcgc ggcaggttat
    73921 gctaccctgt cgcgcaaatt gcttcactct ggagatttcc ctcatgcata tcaacattgc
    73981 ctggcaggac gtagataccg ttctgctgga tatggacggc acgttgctcg acctcgcctt
    74041 cgataactat ttctggcaaa agctggtgcc tgaaacatgg ggcgcgaaaa acggggttac
    74101 gccacaggaa gcgatggaat atatgcgcca gcaatatcac gacgtacagc atacgctaaa
    74161 ctggtactgt cttgattact ggagtgagca actgggtctg gatatctgtg cgatgaccac
    74221 cgagatggga ccgcgcgccg tactgcgtga agataccatt ccgtttcttg aggcactgaa
    74281 agccagcggt aagcagcgaa ttttgctcac caatgctcat ccgcacaacc tggcggtaaa
    74341 acttgagcat acgggtctgg acgcacacct tgatttatta ctttccaccc acacatttgg
    74401 ttatccgaaa gaggatcagc ggttatggca tgcggtggcc gaagctacgg gtctgaaagc
    74461 tgaaagaacg ctgtttattg atgacagcga agcgattctc gatgctgccg cgcaatttgg
    74521 tattcgttac tgcctcggcg tgactaatcc tgattccggg attgccgaga aacagtatca
    74581 acgccatccg tcactgaatg actaccgccg cctgatcccc tcgctaatgt gaaggagacg
    74641 ccatgaaaga gaaacctgct gttgaggttc gactggataa atggctatgg gctgcccgtt
    74701 tttataaaac ccgcgcgctg gcccgtgaaa tgattgaagg cggtaaggtg cattacaacg
    74761 ggcagcgcag caagccgagc aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg
    74821 gaaatgacga acgcacggtg attgtaaagg cgattactga acagcgtcgc cccgccagcg
    74881 aggcagcctt gctgtatgaa gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcga
    74941 tggcacgtaa acttaatgcc ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag
    75001 agcgccgcga cctgttacga tttaaacacg gcgacagtga ataactgtca cctgcaagag
    75061 agatgattat gccgcaacat gaccaattac atcgctatct gtttgaaaat tttgccgttc
    75121 gcggcgaact ggtaaccgtt tcggaaaccc tgcaacagat ccttgagaac cacgattatc
    75181 cgcagcccgt taaaaacgtg ctggcagaac tgctggttgc gaccagcctg ttaaccgcta
    75241 cgctgaagtt tgatggtgat atcaccgtac agctgcaggg cgacggtccg atgaatctgg
    75301 cggttattaa cggtaacaat aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa
    75361 ttccagaaaa tgccgacctg aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta
    75421 ccccgagcga aggcgaacgc tatcagggcg tagttggtct ggaaggtgat accctggcgg
    75481 cctgcctaga agattacttt atgcgttctg aacagctgcc gacgcgcctg tttattcgca
    75541 ccggcgacgt agacggcaaa ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc
    75601 aaaataccca acaggacgac tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg
    75661 aagaactgct gaccttaccg gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg
    75721 tgacggttta cgatccgcag gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg
    75781 ccgatgcgct gaaaacgctg cctgatgaag aagttgatag catcctggcg gaagatggcg
    75841 aaattgacat gcattgtgat tactgcggta accactatct gttcaatgcg atggatattg
    75901 ctgaaatccg caacaacgcg tctccggcag atccgtaagt tcattaatga gtatgtccgg
    75961 cagagagggc tctgccggat tttacgactt aatggcctga tgtgctgcgc ttaccagcgc
    76021 ctatgaaata aatcgcaaat tattttggtc agatacagcg tcgtatccga taaaagcgtt
    76081 gtcacttact taagtttatt tccagcgttt caccatctac cgtcagatcg cgccacgctt
    76141 tcggcctcgt ccccagtttt ccatccttcg cagctgtgag taatttttct ttcgttattt
    76201 cgttcggaag taaactcata tcaagcgcgt cccattcttt tctgtctgag tcaaagccat
    76261 agacaatgac tctgtcatca ttaaaagcaa acaggatccg ctccgcctga ccatcactat
    76321 tcagatcttg ctcgaccagt acgcaggcgt ctttttcaat acaggtcatc acgttatagc
    76381 gatcctggat taaggccgac cagaatgtcg catccggttt aacagaacca ggggcaatta
    76441 acacattttc ggcgaatact ttttccgata cctgttcctg aagatgttgc tctccatcta
    76501 acgccatcag cagatcccgc gcgcgtttcg ggtctttcat aaacccggca tcgcttttca
    76561 gcgactcaag cgccgcacgt ccatagcgac cgctctgttc gagcatgtag atagttacct
    76621 ggtctggcgt gtttttgccg ctctgataac gcgccatatg actgttcacg ctaatacgca
    76681 tactgtccag caccggcgaa ttaagaagca ccagtatcac caacaccaat aacgaaaccg
    76741 caaggttcac tttgccctga agaacaaggg gattttgccc attacgccag acgatactga
    76801 caaaataccc cagcgaccac accagtaaca ccagctccgc cagcgcgccc tgcaagcggt
    76861 cgacagtcca gccgtactga gcgacccgca gccataaccc ccaggtggcg acgaacacat
    76921 acagcggagc aaccagcaaa gcggttttaa tcaggcaacg caacggcccc gtccagggaa
    76981 gtgacgcttt ttgcggatcg cggacaatag ccattaagat caattgcaaa aaggccagcg
    77041 ttaacagcaa cccggcggcg gagatgtggc gagaaatcgc gctcaggccc gtaaacggca
    77101 gggtgatgat aaacatcggg gttagcaatg atactaacgg cagcaacccc gtggcgatta
    77161 gcgtgaacaa cttttgaata gagtcgatta aacgtgactg tgtacgcgcg aggatcaccg
    77221 ccagtgcggt aaccagacca aatgtaagat acataaacca gtcggttgca aaaaagagcg
    77281 tattgaaaaa cgtgatgcca acaagtttaa acaactcacc ccacagtaaa agcaccagcc
    77341 acgtcaggcc attggagaga aaaatcacca gtaatataag tacgttatgc catactgact
    77401 ggtaaaagta gctatagcgg ctactgccat tgcggatacg aaggctttgt tgtatccacg
    77461 gtagcaacaa cattgccatc aacagcagat agcagccaaa atcccaaatc gccttttcag
    77521 ctctccaggg gttcatgcca tcagtttgcc acttcagcca accgctcatc cctgtcgtgg
    77581 caataaacac cagcgccagc catccccaaa ggcgcttctg tttaaaagag atcacggaga
    77641 agagtaaaac ggatgaaaac gcgacggtcg caggcacgcc ataaacaatc caactgtgat
    77701 tttttccggc aagccaggcg ataagcaggt agcaaaccag cccctgaagt aatccggtag
    77761 caatcatccc ccagcgtgtt gccggtgaaa gttcaacgtt atccatgttg gttatccaga
    77821 atcaaaaggt gggttaatta tcgcatccgg gcagtagtat tttgcttttt tcagaaaata
    77881 atcaaaaaaa gttagcgtgg tgaatcgata ctttaccggt tgaatttgca tcaatttcat
    77941 tcaggaatgc gattccactc acaatattcc cgccatataa accaagattt aaccttttga
    78001 gaacattttc cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga
    78061 atcacggagt tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc
    78121 gcggttaaca cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag
    78181 tgaaatgcgc gttaacaatg gtttgccccc gcaagaactc gaggcttatg gtatcagtga
    78241 cgtacatgat atcgtttaca acccaagcta cgacctgctg tatcaggaag agctcgatcc
    78301 gagcctgaca ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac
    78361 cgggatcttc accggtcgtt caccaaaaga taagtatatc gtccgtgacg ataccactcg
    78421 cgatactttc tggtgggcag acaaaggcaa aggtaagaac gacaacaaac ctctctctcc
    78481 ggaaacctgg cagcatctga aaggcctggt gaccaggcag ctttccggca aacgtctgtt
    78541 cgttgtcgac gctttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac
    78601 cgaagtggcc tggcaggcgc attttgtcaa aaacatgttt attcgcccga gcgatgaaga
    78661 gctggcaggt ttcaaaccag actttatcgt tatgaacggc gcgaagtgca ctaacccgca
    78721 gtggaaagaa cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat
    78781 gcagctgatt ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat
    78841 gaactacctg ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggcga
    78901 gaaaggcgat gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca ccctttccac
    78961 cgacccgaaa cgccgcctga ttggcgatga cgaacacggc tgggacgatg acggcgtgtt
    79021 taatttcgaa ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag cggaacctga
    79081 aatctacaac gctatccgtc gtgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg
    79141 cactatcgac tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccaatcta
    79201 tcacatcgat aacatcgtta agccggtttc caaagcgggc cacgcgacta aggttatctt
    79261 cctgactgct gatgctttcg gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac
    79321 ccagtatcac ttcctctctg gcttcaccgc caaactggcc ggtactgagc gtggcatcac
    79381 cgaaccgacg ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac
    79441 tcagtacgca gaagtgctgg tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt
    79501 taacactggc tggaacggca ctggcaaacg tatctcgatt aaagataccc gcgccattat
    79561 cgacgccatc ctcaacggtt cgctggataa tgcagaaacc ttcactctgc cgatgtttaa
    79621 cctggcgatc ccaaccgaac tgccgggcgt agacacgaag attctcgatc cgcgtaacac
    79681 ctacgcttct ccggaacagt ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga
    79741 caacttcgat aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa
    79801 actgtaatga tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga
    79861 ggcaccttcg cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg
    79921 gcactggcag ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca
    79981 tcccgttatg gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa
    80041 tggtgcgcgc actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg
    80101 caattcccgg accgtcatct tccacctgga accaggcgcg attcggctcc gttccgctgc
    80161 tgactttgat ccagccattg ccataacggg cggcgttgac caccatattc gccaccgcgc
    80221 gtttgatcga cagcgggtgc attttcactt caatgctgcc ggggtaaagc gcggtttcaa
    80281 tttcccgctc atagccactt tcggcagcaa tcacctcacc gagcactgca ttaagatccg
    80341 ccatttccat cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg
    80401 cgttgcactc ttcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca
    80461 tcatctcagt cgccaggcga atacgcgtca gcggcgtgcg caagtcgtga cttacccccg
    80521 ccatcagcag cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg
    80581 cacgggtaac ggaacgcacc tccgaagcgc catattcacg cagcggcggc ggaataatcc
    80641 ctttaccaac ctgcaaggct gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa
    80701 taaacagcca cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg
    80761 gagagaaatc gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca
    80821 gccaggtttt cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg
    80881 ggccgcccag ttgctgtgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca
    80941 gacctgcctc ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct
    81001 cccgacggaa agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg
    81061 tcatcaacat acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa
    81121 tcgcgaagtt cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga
    81181 tgagcaataa cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt
    81241 agagccgtcc ggtacaaaga cgtagcccag gccccaaacg gtctgaatgt aacgcggatg
    81301 cgctggatct tcttctacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc
    81361 cattgcggaa tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc
    81421 acgcggatgg ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg
    81481 ctcatcttcg cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat
    81541 taccgcctct tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc
    81601 acgaatacgg gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc
    81661 aatctccagg cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg
    81721 catcgggttg ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa
    81781 cattaaatcc agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc
    81841 tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc
    81901 acgcaggcgc atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac
    81961 tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc
    82021 agctaaatct ggtataaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta
    82081 agtatacaat ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca
    82141 atgtgcgcat tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc
    82201 gggaagggta tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg
    82261 aggtcacaaa aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact
    82321 atcagtataa taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat
    82381 gcctggaaaa tctcaaaatc gtcgattact cccctcagca ggaaggcaaa gtattttttg
    82441 gcgcgtgggt ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct
    82501 acgatgaaat ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat
    82561 tgctgaaaaa agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgaact
    82621 ggtatgttaa tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat
    82681 ggctggcatt tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat
    82741 gaactcaaac catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc
    82801 gcccggaaca ggttgacgct gccgttcgcc tgcttgacga agggaatacc gtgccgttta
    82861 tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg
    82921 aaacgcgtct gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca
    82981 tttccgagca aggcaaactc accgatgatc tggcgaaggc catcaacgcc accctaagca
    83041 aaaccgaact cgaagacctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa
    83101 tcgccattga agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca
    83161 cgccagaagt cgccgctgca caatatgttg atgccgataa aggcgtggca gataccaaag
    83221 ccgcgctgga tggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc
    83281 tggcgaaagt gcgtgattat ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg
    83341 gtaaagaaga ggaaggggcg aaattccgcg actatttcga tcatcacgaa ccgttgtcca
    83401 cggtgccttc tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc
    83461 tttcgctgaa tgccgatccg cagttcgatg agccgcccaa agagagctat tgcgagcaaa
    83521 tcatcatgga tcaccttggc ctgcgcctga acaatgcccc ggcggatagc tggcgcaaaa
    83581 gcgtagtgag ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg
    83641 gtaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg
    83701 atctgctgat ggcggcccct gccggactgc gtgcaacgat gggcctcgat ccgggtctgc
    83761 gtaccggggt aaaagtggcg gtggtcgatg ccactggcaa actggtggcg accgatacca
    83821 tttacccgca caccggacaa gccgcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa
    83881 aacataacgt tgaactggtg gcgatcggca acggtacagc ttcccgcgaa actgaacgtt
    83941 tctatctcga cgtgcagaag cagttcccga aagtgaccgc acagaaagtg atcgtcagcg
    84001 aaactggcgc gtcggtttac tcagcttccg agctggcagc gcaggagttc ccggatctcg
    84061 acgtttcgct gcgtggtgcg gtatctatcg cccgccgttt gcaggatccg ctggcggagc
    84121 tggtgaaaat cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga
    84181 cgcaactggc ccgcaaactg gatgcagtag tagaagactg cgtaaacgcc gttggcgtcg
    84241 atctcaacac cgcttctgtt ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg
    84301 cgcaaaacat cgtggcctgg cgcgatgaga acggccagtt ccagaaccgt cagcaactgc
    84361 tgaaagtcag ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta
    84421 accacggtga taacccgctg gacgcctcta ccgttcatcc ggaagcctat ccggtggtgg
    84481 aacgcattct ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac
    84541 tgcgtaacct gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg
    84601 acatcatcaa agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc
    84661 agtttgccga tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg
    84721 cagtgaccaa cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc
    84781 tggttcacat ctcttcatta tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag
    84841 cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc
    84901 tgactatgcg tctggacgag cagcctggcg aaaccaactc tcgtcgcggc ggcggtaatg
    84961 aacgcccgca aaacaaccgc ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg
    85021 gtaatagcgc gatgatggat gcgctggcgg cagcgatggg taaaaaacgt taaacgcccg
    85081 gactggcccc taaacctgcg cgataaggcg ttcacgccgc atctggcaac gaacgctttg
    85141 cctgacataa aagtgccgga gaatatctcc ggcactttta ttccacagcc aaactcacaa
    85201 tatactccgt taatatttat cacttcatta acaactgaaa ccttaattaa acattagcca
    85261 gtccgggtaa ctcactattc gaattatatt ttcgctgcga tataaccttg agccacatca
    85321 acattgagtc agattattat tcaaaccaac attcgcacac attttaagta ttgctgatag
    85381 aaaccattct cattatcatt gtgttgttga ttatttaatc tctcctttgt tggcaaatca
    85441 tctggtctca tgtcgcggtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa
    85501 gtaggcacct atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga
    85561 aatcagcccg gcatatcgcc aaaaactgct ttctctaggc atgttacctg gctcctcttt
    85621 tgatgtggtg cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag
    85681 cctggtatta cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata
    85741 cggtgataac aacaatgaaa aaattaacca ttggcttaat tggtaatcca aattctggca
    85801 agacaacctt atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg
    85861 ttaccgtcga acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg
    85921 acctgcccgg cacctattct ctgaccacca tctcatcgca gacctcgctc gatgagcaaa
    85981 tcgcctgtca ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt
    86041 ctaaccttga gcgtaacctg tacctgacgc tgcaactgct ggaactcggc attccctgca
    86101 tcgtggcgct gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg
    86161 ctctatcggc acgtcttggc tgcccggtga tcccgctggt ttcaacccgt ggtcgcggta
    86221 ttgaagcgct caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc
    86281 attacgcaca gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca
    86341 tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc
    86401 gcgcctacgc cggtgaagcg tcgcagcatc tggatgccac cctcgcccgt ctgcgtaatg
    86461 aaatggatga tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct
    86521 gtgatgtggt aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata
    86581 aaatcgtgct caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt
    86641 tcctgctggc tatcaacatc ggcggggcgt tacagccgct gtttaacgtc ggctccgtgg
    86701 cgctatttgt gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga
    86761 ctatcttcct cgcccagggc ctgggtggcg gtattaacac cgtgctgcca ctggtgccgc
    86821 agatcggcat gatgtacctg ttcctctcct tccttgagga ctccggctat atggcgcgtg
    86881 cagcgtttgt gatggaccgc ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc
    86941 cgctgatcgt cggtttcggt tgtaacgtac cgtcggtaat gggcgcacgt acgcttgatg
    87001 caccgcgtga acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc
    87061 tggctatctt cgcagtattc gcggctgcct tcttcgggca gaacggtgcc ctggcggttt
    87121 tctcgctgta tatgctcggt atcgtgatgg cggtgctgac tggcctgatg ctcaagtaca
    87181 ccatcatgcg cggtgaagcg acgccgtttg tcatggagct gccggtctac catgtaccac
    87241 acgttaaaag cctgattatc cagacctggc agcgtctgaa gggcttcgtt ctgcgtgctg
    87301 gtaaagtgat tatcatcgtc agcattttcc tgagcgcttt caacagcttc tcgttgagcg
    87361 ggaaaatcgt cgataacatc aacgactcgg cgctggcttc cgtcagccgg gtgataaccc
    87421 ctgtcttcaa gccgattggc gtacatgaag ataactggca ggcaacggtt ggcctgttta
    87481 cgggcgcaat ggcaaaagaa gtggtggtgg gtacgctcaa caccctctac accgcagaaa
    87541 atattcagga cgaagagttc aatccggcgg aattcaacct cggtgaagag ctgttcagtg
    87601 cggtagatga aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc
    87661 ccattgaagc tagcaaaggc aacggtgaaa tgggtaccgg ggcgatgggc gtgatggatc
    87721 agaaattcgg tagcgcagct gccgcataca gctacctgat tttcgtcctg ctgtatgtac
    87781 catgtatctc ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg atgggcttct
    87841 ccatcctgtg ggggctgaat atcgcttact cactggcaac attgttctat caggtcgcca
    87901 gctacagtca gcacccaact tacagcctgg tgtgcattct ggcggtaatc ctgtttaaca
    87961 tcgtagctat cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa
    88021 cccgcaagtc ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc
    88081 ttcacttatt caggtacgtg atttgctggc gttacggggc cgtatggaag cgacccagat
    88141 aagccagaca ttgaacactc cgcagccaat gattaacgcc atgctgcaac agctggaaag
    88201 catgggcaaa gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa
    88261 aagctgcccg gaaggcaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccatactc
    88321 catcgcctgt tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt
    88381 tccataaaaa tgcaattctt gcagcgcaac gtaagttcct ctgaaagcgt ctcgcaggga
    88441 tgaaaactcg ctaatgcaca gatgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca
    88501 ggtgaaggaa cgccatgagt aaaaagcaga gttccacccc acatgatgcg ctgttcaaac
    88561 tctttttacg ccaaccggag acggcacgcg attttcttgc gtttcattta ccggcaccca
    88621 ttcacgcgct ctgtgatatg aaaaccctca agctggagtc gaggagcttt attgatgacg
    88681 atctgcgtga aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat
    88741 acatctattg tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga
    88801 tgcgttacgc tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga
    88861 tggtggtgcc attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtgtt
    88921 ggctggattg tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc
    88981 tgattgatgt caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc
    89041 tggagttaat tcaaaaacat attcgtcaac gcgatctgat ggggctggta gagcaaatgg
    89101 cctgcttatt aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca
    89161 tactgcaaac tggcgacgct gtacgtttta acgattttat cgacggcgtt gccgaacgtt
    89221 caccgaaaca caaggagagt ttaatgacta ttgcggaaag attgcggcag gagggggaac
    89281 aatccaaagc cctgcatata gccaaaataa tgcttgaatc cggagtccct cttgcagaca
    89341 tcatgcgatt taccgggctg tcagaagaag agttggctgc ggcgagtaga ctggccccct
    89401 gaatctccag acaaccagta t
//
LOCUS       DAECNO010000002        78784 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.002, whole genome shotgun sequence.
ACCESSION   DAECNO010000002 DAECNO010000000
VERSION     DAECNO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.002"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..229
                     /locus_tag="KKO08_000105"
     CDS             <1..229
                     /locus_tag="KKO08_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HBD6316524.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            649..1264
                     /locus_tag="KKO08_000106"
                     /pseudo
     CDS             649..1264
                     /locus_tag="KKO08_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1367..1690
                     /locus_tag="KKO08_000107"
     CDS             1367..1690
                     /locus_tag="KKO08_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HBD6316525.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1757..3214)
                     /gene="nuoN"
                     /locus_tag="KKO08_000108"
     CDS             complement(1757..3214)
                     /gene="nuoN"
                     /locus_tag="KKO08_000108"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBD6316526.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3221..4750)
                     /gene="nuoM"
                     /locus_tag="KKO08_000109"
     CDS             complement(3221..4750)
                     /gene="nuoM"
                     /locus_tag="KKO08_000109"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBD6316527.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4914..6755)
                     /gene="nuoL"
                     /locus_tag="KKO08_000110"
     CDS             complement(4914..6755)
                     /gene="nuoL"
                     /locus_tag="KKO08_000110"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBD6316528.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6752..7054)
                     /gene="nuoK"
                     /locus_tag="KKO08_000111"
     CDS             complement(6752..7054)
                     /gene="nuoK"
                     /locus_tag="KKO08_000111"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBD6316529.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7051..7605)
                     /gene="nuoJ"
                     /locus_tag="KKO08_000112"
     CDS             complement(7051..7605)
                     /gene="nuoJ"
                     /locus_tag="KKO08_000112"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBD6316530.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7617..8159)
                     /gene="nuoI"
                     /locus_tag="KKO08_000113"
     CDS             complement(7617..8159)
                     /gene="nuoI"
                     /locus_tag="KKO08_000113"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBD6316531.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8174..9151)
                     /gene="nuoH"
                     /locus_tag="KKO08_000114"
     CDS             complement(8174..9151)
                     /gene="nuoH"
                     /locus_tag="KKO08_000114"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBD6316532.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9148..11874)
                     /gene="nuoG"
                     /locus_tag="KKO08_000115"
     CDS             complement(9148..11874)
                     /gene="nuoG"
                     /locus_tag="KKO08_000115"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBD6316533.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11927..13264)
                     /gene="nuoF"
                     /locus_tag="KKO08_000116"
     CDS             complement(11927..13264)
                     /gene="nuoF"
                     /locus_tag="KKO08_000116"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBD6316534.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13261..13761)
                     /gene="nuoE"
                     /locus_tag="KKO08_000117"
     CDS             complement(13261..13761)
                     /gene="nuoE"
                     /locus_tag="KKO08_000117"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBD6316535.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13764..15566)
                     /gene="nuoC"
                     /locus_tag="KKO08_000118"
     CDS             complement(13764..15566)
                     /gene="nuoC"
                     /locus_tag="KKO08_000118"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBD6316536.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15660..16322)
                     /gene="nuoB"
                     /locus_tag="KKO08_000119"
     CDS             complement(15660..16322)
                     /gene="nuoB"
                     /locus_tag="KKO08_000119"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBD6316537.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16338..16781)
                     /gene="nuoA"
                     /locus_tag="KKO08_000120"
     CDS             complement(16338..16781)
                     /gene="nuoA"
                     /locus_tag="KKO08_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBD6316538.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17412..18350)
                     /gene="lrhA"
                     /locus_tag="KKO08_000121"
     CDS             complement(17412..18350)
                     /gene="lrhA"
                     /locus_tag="KKO08_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBD6316539.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19270..20487
                     /gene="alaA"
                     /locus_tag="KKO08_000122"
     CDS             19270..20487
                     /gene="alaA"
                     /locus_tag="KKO08_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBD6316540.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20571..21170
                     /gene="yfbR"
                     /locus_tag="KKO08_000123"
     CDS             20571..21170
                     /gene="yfbR"
                     /locus_tag="KKO08_000123"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBD6316541.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21229..23061)
                     /locus_tag="KKO08_000124"
     CDS             complement(21229..23061)
                     /locus_tag="KKO08_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBD6316542.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23148..23798)
                     /gene="hxpA"
                     /locus_tag="KKO08_000125"
     CDS             complement(23148..23798)
                     /gene="hxpA"
                     /locus_tag="KKO08_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBD6316543.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23809..24303)
                     /locus_tag="KKO08_000126"
     CDS             complement(23809..24303)
                     /locus_tag="KKO08_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBD6316544.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24386..24841)
                     /locus_tag="KKO08_000127"
     CDS             complement(24386..24841)
                     /locus_tag="KKO08_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBD6316545.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25179..26381
                     /gene="ackA"
                     /locus_tag="KKO08_000128"
     CDS             25179..26381
                     /gene="ackA"
                     /locus_tag="KKO08_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBD6316546.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26456..28600
                     /gene="pta"
                     /locus_tag="KKO08_000129"
     CDS             26456..28600
                     /gene="pta"
                     /locus_tag="KKO08_000129"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBD6316547.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28790..30310
                     /gene="yfcC"
                     /locus_tag="KKO08_000130"
     CDS             28790..30310
                     /gene="yfcC"
                     /locus_tag="KKO08_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBD6316548.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30343..30885)
                     /gene="yfcD"
                     /locus_tag="KKO08_000131"
     CDS             complement(30343..30885)
                     /gene="yfcD"
                     /locus_tag="KKO08_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBD6316549.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30943..31494)
                     /gene="yfcE"
                     /locus_tag="KKO08_000132"
     CDS             complement(30943..31494)
                     /gene="yfcE"
                     /locus_tag="KKO08_000132"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBD6316550.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31550..32194)
                     /gene="yfcF"
                     /locus_tag="KKO08_000133"
     CDS             complement(31550..32194)
                     /gene="yfcF"
                     /locus_tag="KKO08_000133"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBD6316551.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32330..32977
                     /gene="yfcG"
                     /locus_tag="KKO08_000134"
     CDS             32330..32977
                     /gene="yfcG"
                     /locus_tag="KKO08_000134"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBD6316552.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33034..33396
                     /gene="folX"
                     /locus_tag="KKO08_000135"
     CDS             33034..33396
                     /gene="folX"
                     /locus_tag="KKO08_000135"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBD6316553.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33417..34310
                     /locus_tag="KKO08_000136"
     CDS             33417..34310
                     /locus_tag="KKO08_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBD6316554.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34358..35248)
                     /gene="rpnB"
                     /locus_tag="KKO08_000137"
     CDS             complement(34358..35248)
                     /gene="rpnB"
                     /locus_tag="KKO08_000137"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBD6316555.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35445..36218)
                     /gene="hisP"
                     /locus_tag="KKO08_000138"
     CDS             complement(35445..36218)
                     /gene="hisP"
                     /locus_tag="KKO08_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBD6316556.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36226..36942)
                     /locus_tag="KKO08_000139"
     CDS             complement(36226..36942)
                     /locus_tag="KKO08_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6316557.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36939..37625)
                     /gene="hisQ"
                     /locus_tag="KKO08_000140"
     CDS             complement(36939..37625)
                     /gene="hisQ"
                     /locus_tag="KKO08_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBD6316558.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37715..38497)
                     /gene="hisJ"
                     /locus_tag="KKO08_000141"
     CDS             complement(37715..38497)
                     /gene="hisJ"
                     /locus_tag="KKO08_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBD6316559.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38718..39500)
                     /gene="argT"
                     /locus_tag="KKO08_000142"
     CDS             complement(38718..39500)
                     /gene="argT"
                     /locus_tag="KKO08_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBD6316560.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39766..40335)
                     /gene="ubiX"
                     /locus_tag="KKO08_000143"
     CDS             complement(39766..40335)
                     /gene="ubiX"
                     /locus_tag="KKO08_000143"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBD6316561.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40430..41947)
                     /gene="purF"
                     /locus_tag="KKO08_000144"
     CDS             complement(40430..41947)
                     /gene="purF"
                     /locus_tag="KKO08_000144"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBD6316562.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41984..42472)
                     /gene="cvpA"
                     /locus_tag="KKO08_000145"
     CDS             complement(41984..42472)
                     /gene="cvpA"
                     /locus_tag="KKO08_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBD6316563.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42731..43393)
                     /gene="dedD"
                     /locus_tag="KKO08_000146"
     CDS             complement(42731..43393)
                     /gene="dedD"
                     /locus_tag="KKO08_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBD6316564.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43383..44651)
                     /gene="folC"
                     /locus_tag="KKO08_000147"
     CDS             complement(43383..44651)
                     /gene="folC"
                     /locus_tag="KKO08_000147"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBD6316565.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44721..45635)
                     /gene="accD"
                     /locus_tag="KKO08_000148"
     CDS             complement(44721..45635)
                     /gene="accD"
                     /locus_tag="KKO08_000148"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBD6316566.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45791..46450)
                     /locus_tag="KKO08_000149"
     CDS             complement(45791..46450)
                     /locus_tag="KKO08_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6316567.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46533..47345)
                     /gene="truA"
                     /locus_tag="KKO08_000150"
     CDS             complement(46533..47345)
                     /gene="truA"
                     /locus_tag="KKO08_000150"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBD6316568.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47345..48358)
                     /locus_tag="KKO08_000151"
     CDS             complement(47345..48358)
                     /locus_tag="KKO08_000151"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD6316569.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48424..49560)
                     /gene="pdxB"
                     /locus_tag="KKO08_000152"
     CDS             complement(48424..49560)
                     /gene="pdxB"
                     /locus_tag="KKO08_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBD6316570.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49659..50654
                     /gene="flk"
                     /locus_tag="KKO08_000153"
     CDS             49659..50654
                     /gene="flk"
                     /locus_tag="KKO08_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBD6316571.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50651..51829)
                     /locus_tag="KKO08_000154"
     CDS             complement(50651..51829)
                     /locus_tag="KKO08_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6316572.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52084..53325)
                     /gene="fabB"
                     /locus_tag="KKO08_000155"
     CDS             complement(52084..53325)
                     /gene="fabB"
                     /locus_tag="KKO08_000155"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBD6316573.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53484..55490
                     /gene="mnmC"
                     /locus_tag="KKO08_000156"
     CDS             53484..55490
                     /gene="mnmC"
                     /locus_tag="KKO08_000156"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBD6316574.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55611..55889)
                     /locus_tag="KKO08_000157"
     CDS             complement(55611..55889)
                     /locus_tag="KKO08_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBD6316575.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55923..56471)
                     /gene="epmC"
                     /locus_tag="KKO08_000158"
     CDS             complement(55923..56471)
                     /gene="epmC"
                     /locus_tag="KKO08_000158"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBD6316576.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56471..57280)
                     /locus_tag="KKO08_000159"
     CDS             complement(56471..57280)
                     /locus_tag="KKO08_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBD6316577.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57280..58104)
                     /gene="mepA"
                     /locus_tag="KKO08_000160"
     CDS             complement(57280..58104)
                     /gene="mepA"
                     /locus_tag="KKO08_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBD6316578.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58108..59193)
                     /gene="aroC"
                     /locus_tag="KKO08_000161"
     CDS             complement(58108..59193)
                     /gene="aroC"
                     /locus_tag="KKO08_000161"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBD6316579.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59228..60160)
                     /gene="prmB"
                     /locus_tag="KKO08_000162"
     CDS             complement(59228..60160)
                     /gene="prmB"
                     /locus_tag="KKO08_000162"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD6316580.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60326..60877
                     /gene="smrB"
                     /locus_tag="KKO08_000163"
     CDS             60326..60877
                     /gene="smrB"
                     /locus_tag="KKO08_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBD6316581.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60981..61871)
                     /locus_tag="KKO08_000164"
     CDS             complement(60981..61871)
                     /locus_tag="KKO08_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBD6316582.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61858..62382)
                     /locus_tag="KKO08_000165"
     CDS             complement(61858..62382)
                     /locus_tag="KKO08_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6316583.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62379..62849)
                     /locus_tag="KKO08_000166"
     CDS             complement(62379..62849)
                     /locus_tag="KKO08_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6316584.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62846..63394)
                     /locus_tag="KKO08_000167"
     CDS             complement(62846..63394)
                     /locus_tag="KKO08_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6316585.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63399..64121)
                     /locus_tag="KKO08_000168"
                     /pseudo
     CDS             complement(63399..64121)
                     /locus_tag="KKO08_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64141..64863)
                     /locus_tag="KKO08_000169"
     CDS             complement(64141..64863)
                     /locus_tag="KKO08_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD6316586.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64923..66155)
                     /locus_tag="KKO08_000170"
     CDS             complement(64923..66155)
                     /locus_tag="KKO08_000170"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD6316587.1"
                     /translation="MRADWQTNYQHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLK
                     AKLALGEDYLRSDIFDGFNYVGGSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQM
                     GRVIYETQVPAGPFRIQDLGDSVSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVR
                     YKIMMGRPQEWGHHVEGEFFSGAEASWGIANGWSLYGGALGDENYQSAALGVGRDLST
                     FGAVAFDVTHSHTKLDKDTAYGKGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENF
                     MTMSEYLDASDSGMVRTGNDKEMYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYN
                     IMLSHYFNMGSIRNVSISMTGYRYEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGR
                     TAVRSVISAVSMTRLTIS"
     gene            complement(66204..66782)
                     /locus_tag="KKO08_000171"
                     /pseudo
     CDS             complement(66204..66782)
                     /locus_tag="KKO08_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66864..67427)
                     /locus_tag="KKO08_000172"
     CDS             complement(66864..67427)
                     /locus_tag="KKO08_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6316588.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68111..68596)
                     /gene="sixA"
                     /locus_tag="KKO08_000173"
     CDS             complement(68111..68596)
                     /gene="sixA"
                     /locus_tag="KKO08_000173"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBD6316589.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68799..70943)
                     /gene="fadJ"
                     /locus_tag="KKO08_000174"
                     /pseudo
     CDS             complement(68799..70943)
                     /gene="fadJ"
                     /locus_tag="KKO08_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            complement(70943..72253)
                     /gene="fadI"
                     /locus_tag="KKO08_000175"
     CDS             complement(70943..72253)
                     /gene="fadI"
                     /locus_tag="KKO08_000175"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBD6316590.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72433..72717)
                     /locus_tag="KKO08_000176"
     CDS             complement(72433..72717)
                     /locus_tag="KKO08_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBD6316591.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73089..74429
                     /gene="fadL"
                     /locus_tag="KKO08_000177"
     CDS             73089..74429
                     /gene="fadL"
                     /locus_tag="KKO08_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBD6316592.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75180..75854
                     /locus_tag="KKO08_000178"
     CDS             75180..75854
                     /locus_tag="KKO08_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316593.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLFLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTKYQNEINQLSALG
                     VNFDDI"
     gene            complement(76036..76791)
                     /gene="mlaA"
                     /locus_tag="KKO08_000179"
     CDS             complement(76036..76791)
                     /gene="mlaA"
                     /locus_tag="KKO08_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBD6316594.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            77085..78017
                     /locus_tag="KKO08_000180"
     CDS             77085..78017
                     /locus_tag="KKO08_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBD6316595.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            78091..78165
                     /locus_tag="KKO08_000181"
     tRNA            78091..78165
                     /locus_tag="KKO08_000181"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78124..78126,aa:Arg,seq:cct)
     gene            78327..>78784
                     /gene="intS"
                     /locus_tag="KKO08_000182"
     CDS             78327..>78784
                     /gene="intS"
                     /locus_tag="KKO08_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBD6316596.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT        19966 a        21040 c        19326 g        18452 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaataa
      601 cgccttttat tgataattta catatgaaaa aaattttcct tttgttatat tatttctgtt
      661 ttcactccct ctgattattt ttaccaaccc tgggtcactg cattatcgcc aacgccccga
      721 cacgtaagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac tgtgcatcca
      781 cgtaatgccg gcaatattga taatctgaat aggtccgcag agtatataaa agagttcttt
      841 gtcagtagcg gtgccagagt taccgcgcag ggcgtccccg attggctatt acgactctgc
      901 gcctggaagc cagaattacc cttacccggc aatgtcctgg ctttatcccg atcggggaga
      961 ctttattgct gtggtcggca gaatgcagga tatcaacgcc gttcgtcagg taaaagcggc
     1021 attgttgtca tcccgggatt tatctgttta ttctataaat gccccagggt ttattcccgg
     1081 tattgatttt tctgatcacc tcaattattg gcaacacgat attcccgccg taatgattac
     1141 tgacaccgct ttttatcgta ataaacaata ccacttgccc ggtgataccg cagacagatt
     1201 gaattatcag aaaatggctc aggtggtaga tggtgttata accttgttat acaacagtaa
     1261 ataataaaaa tttctcgagg cgggtccaga aacagcgaca tgtacatttt attttccctc
     1321 gtctgtttta agatgcgaaa cataaaattc aatggaggaa aaataaatgg gaatgattgg
     1381 ctattttgca gaaattgact cagaaaagat taaccaactg cttgagtcta ctgaaaaacc
     1441 tttgatggat aatatacatg acacgctttc aggattacgc cgactggaat cactggatcg
     1501 aagtgagctg cgtaaacagt tttccattaa gcgactcaat gaaatggaga tttatccggg
     1561 agtgaccttt tcagaagagt tagaggggca actctttgcc tcgatcatgc ttgatatgga
     1621 aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc tgacggttat
     1681 tgttgggtga tggtgtcaga gcgccaaaaa aaaccgccga acatgtcggc ggctttctga
     1741 cttacaaact aacagtttac atcagcggca ttgctaaccg cacaatacta atcagcggtt
     1801 gtggccatac acccagcacc agtaccaaca gtgcagaaat cagcaccacg ataccgcccg
     1861 cgctgtactg ccagtttgac ggtgcatcgc gacccggttg ttccggtgcg tgcaggtaca
     1921 ggctcaccgc cacgcgcagg tagtagtaga ggccgattgc cgaaccgaca accacggcac
     1981 ccaccagcca ccacaagtgt gcctggacac cgaccgccag cacgtagaac ttaccgataa
     2041 agcccagcgt catcgggata ccggccagag acagcatcat caccgtcatc actgccgcga
     2101 ggatcggacg atgccagaac agaccgcggt aagagaacag ggaatcagca tccgggccac
     2161 gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg aacagataac
     2221 cggccaggta aacccctacc gcttccatcg acatctcgcc ggtttgcagc gcaatcagcg
     2281 ccaccagcag atagccgagg tgagagatag atgagtaacc gagcagacgt ttgatgttgg
     2341 tctggctcaa cgccatcagg ttaccgaaga tgatggaggc aaaggcgata atcgccagta
     2401 ctacgcgaat cgcttcgctg tcacccaccg gtgcgtagag gaacagacgc atcaccacac
     2461 cgaagatagc gattttgctc gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct
     2521 ggtatacgtc tggcgtccac aggtggaacg gcaccagaga gagtttgaag ccgaggccaa
     2581 caatcatcat gccgaaacct gccagcaaca gcggctcgtt gagcataccg tcgccgaggt
     2641 ttttacccag cgcgacaaac gacaggtcgc cagactgcgc atacaccagc gccataccga
     2701 acagcaggaa agaagacgct gcggcagaaa ggatggtgta tttgatactg gcttccagcg
     2761 aacgtttctg gcggaaagcg taaccgacca ggccaaacag cggcaaagag atcagttcaa
     2821 taccgaggaa cagagacgcc agatggtttg cattcgccag caggatcccg cccagcgcgg
     2881 caattaacac caacaggtag aactcatcct tgttgtcgtt atagccttca agccacgggt
     2941 aggcgaaagt acaggtggcg aggctcgcca acaataccag cccggtgtaa agcatggcga
     3001 aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc cgcctggcca acaaaccaga
     3061 gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt agcgttgagg aaatgattgc
     3121 gtcgccacgc aatggagagc atcacaacca ccaccgtcaa gccgacgatc agcaacggta
     3181 gcagtgcgat caggttttgt ggagttattg tcatggcgat ttacggcctt gtagtagtaa
     3241 cggaattaac aaaccactgc tggatattgc caatcgcgga gtgcgaggta tccagaatcg
     3301 gctgcggata gaagcccagc agtaccagca gcaccaccag caacaggatc ataaacagct
     3361 cacgcagcga catccctggc agttcctggc tggcaatctg gcttttcgct ttaccgaagt
     3421 aagcgcgatg caacatcgcc agcgaataaa cagatgcaaa gaccagccca aaggtagaga
     3481 taacggtaat caccgggaca acctggaagc tgccgaacag aatcataaat tcgccgacga
     3541 agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca
     3601 gccatttcat cttgctccac agaccgccca tcatgcgcat gtcgcgggta tggatacgtt
     3661 cataaagctg accgcagaga ataaacagac ccgccgccga caagccgtgc gcaatcatct
     3721 ggattaccgc gccctggtag gccaactggc tgccggtgta gattgcaatc agcacgaagc
     3781 ccatgtggga aaccgaggtg taggcgatca gacgtttgat atcggtctga gcgaaggtca
     3841 tccacgcacc gtagaagatg ccgataacac ccagccacat agcgattggc gcgaactctg
     3901 ccgacgcgtt cgggaacagc ggcagggaga aacgcaacaa accgtaagcg gcggttttca
     3961 gcaagatccc cgcgaggtcc acggaaccgg cggtcggagc ctgggagtgc gcatccggca
     4021 gccagccatg cagcggaacc accggcattt tgactgcgaa ggcgatgaag aagcccagca
     4081 tcaacagata ttccacacca ctggacattg gcgtattcag cagctcttca tagttgaagg
     4141 tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc caggatggcg atcaacatca
     4201 ccagaccact cgcctgggtg taaatgaaga acttggttgc cgccgtgata cgcgttttac
     4261 cgtcagaggc tttatgcccc cacagtgcga tcaggaagta catcggcacc agcatcattt
     4321 cccagaagaa gaagaacagg aacatgtcga tggcaaggaa cacgccgata acgccgccca
     4381 ggatccacat caggttgagg tggaagaagc cctgatattt ttcgatctct ttccacgaac
     4441 atagtaccgc cagcacaccg agcagaccgg tcagcacgac catcagcagc gacagaccgt
     4501 caatggcgag atgaatagag ataccaaaac gcgggatcca cggcatgtcg aattcagact
     4561 gccactgcgg aattccggcg gattgcgtca gtgaataacc gccctgcaac cacagttgca
     4621 gcgacagcgc cagcgtcaat cccatggtga tcagcgcgat ccagcgtggc accttgacgc
     4681 caaagcgttc ggtctgccag cacaggaagc cgccaataaa gggaattaat attagccagg
     4741 gtagtaacat ggcgatcttt attccttgta aaagtcccgt caggaccgga ttttcaacga
     4801 attctcacga cagaaaattc tcttcggact gggggtagtg tcggatgcgc cgcgaaggca
     4861 gcttatccga cctacgttct tatatgccca ggggcaatcc cacaatcctt aactcaacgc
     4921 agtaccatca acagtgccag caccacgacc gcaccgatgc tcatggatgc cacataccag
     4981 cgcagataac cgttctcgct taacagcaga cctttacctg caaagcggga aaaaaccgcc
     5041 gggatgttca tcattgagtt cagcggatcg cgtttcagca accaggcaat acccaggaac
     5101 ggcttgacga acactttgtc gtacagccag tcaaagcccc aagcgttgta ccaccaggta
     5161 ctcagcaggc ggcccggcgc actgttggcg atggaggtca ccagagaacg tttacccagc
     5221 cacagccagg ctgccagcag aatgccgacc accgcgacca cgccagaggt aatttccagg
     5281 gtcaacatgc tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg cagcggcggt
     5341 acaatcagtg cgccaacgaa ggtggaaagg atcagcagca caatcagcgg caggctgtga
     5401 gttacccctt tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc
     5461 atacggaagg tgtagagcga ggtcataaac gcaccgacca gacctgccac catcagattg
     5521 atatgaccat tcgccatcgc acccgcgagg atctcatcct tactgaagaa gcccgcagtg
     5581 accagcggca gtgccgacag tgctgcgccg cccaccagga agcagagata aaccagcgga
     5641 atagatttac gcagaccgcc catcttgaag atgttctgtt cgtgatggca ggccagaatg
     5701 acggaaccgg atgccaggaa cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa
     5761 atcgccgcat cccatgcctg cacgccaagc gcgaggaaca tgtagccaat ctggctcatg
     5821 gtagagtaag cgagaacacg tttgatgtcg gtctgtacca gcgcggcaaa accggccagc
     5881 agcagcgtaa ccgccccgac aatacccacc agatgcagaa cttccggcgt catcaggaac
     5941 aggccgtggg tacgggcgat caggtagaca cccgcggtta ccatcgttgc ggcgtggatc
     6001 agcgcggaga caggcgtcgg gcccgccatc gcgtcagcaa gccatgtctg caacggcaac
     6061 tgcgcagatt taccgaccgc accgcccagc agcatcagcg tcgcccacat cagcatgtta
     6121 ttgccgtcag caaagtgcgc tggtgccagt tccaccattt cgcggaagtt cagggtgccc
     6181 agttcgttgt aaagaatgaa cagtgcgaaa gcgaggaaaa cgtcacccac acgggtcacg
     6241 acgaacgctt tcattgctgc tgcgccattc ttcggatcgg tgtaatagaa accgatcagc
     6301 agataggagc acaggcccac gccttcccag ccgaggtaca tcagcagcag gttatcggcg
     6361 agaaccagaa ctaccatgct ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag
     6421 ccctcttcac cgcgcatgta ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg
     6481 accaccgaga gcatggtcag cgacaggccg tccagcacca ggttaaaacc gatgttaaag
     6541 tcgcctaccg acatccacgt ccacagcggc tggctgtatg cctgctcgcc gttagcgaag
     6601 aaatcaacgc cgataaaggc ggttaccagc gccgccaggc ccacagagcc aacgccgacg
     6661 atcgccgaaa cgttttcaga ccagcgccca cgggagaacg ccagcaggac gaagccaatc
     6721 aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg catctcactt actgaatcga
     6781 tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg atactcgctt
     6841 ctgccgccgc gaggctgatg gcgagaatgt acatcacctg accgtcggtc tgaccccagt
     6901 agcttccggc gaccacaaag gccagcgcgg aggcgttaat catgatttcc agaccaatca
     6961 gcataaacag cagattgcga cggataacca gaccggttaa gccaagaacg aataagattg
     7021 ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc
     7081 gctgtcgtct ttacgattgc tcagcacttc acccgcacgc tcttcacgac cgacgtggaa
     7141 ggccacaacc agacccgcga gcagcagcat agaagccagt tccaccgcca gtacgtaagg
     7201 cccgaacagc gtaataccca ctgctttagc actgattggc gtaccgtcga taccctgatc
     7261 gttaacaccg aggatggcgt aaacaatcac caccagcatg atggccgaca aaattgccgg
     7321 gccaatccac acctgcggtt tcagccactg gcgttcctgt tcgatttctg aaccgcccag
     7381 gttgagcatc atcaccacga acacgaacag caccataatg gcacccgcgt agacgataat
     7441 ttccagcgca ccggcgaagt aagcgcctag tgagaagaac accccggaaa tcgccagcag
     7501 cgaaataatc aggtacagca gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt
     7561 cgcaagtatg gctatcaggc cacagatata aaaagcgaac tccattgccc ctctccttac
     7621 ggtaacaggc tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc cttatctttg
     7681 ccgtcgattg ccatacctgc catccggtag aagttatatt ccgggtattt gcccggaccg
     7741 gagatcagca gatcctcttt ctcgtaaacc agatcctggc gcttgtattc ccccatttcg
     7801 aaatccgggg ttaactgaat cgccgtggtc ggacaggctt cttcgcacag accacagaaa
     7861 atgcagcgtg agaagttaat gcggaaaaat tccggatacc agcgaccgtc tttggtttct
     7921 gctttttgca gcgagataca gccgaccggg caggctaccg cacagaggtt acaggctacg
     7981 caacgctctt cgccgtccgg gtcgcgggtc agaacgatac gaccacgata acggggcggc
     8041 agatagaccg gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg
     8101 atcatccaga tactacgaac ctgggtgccg aaacctacta acaattcttt taaggtcatg
     8161 gtcttattgc cccttattgc gcctgccaga gaatgacagc cgccgttacc agcaagttga
     8221 tcagcgtcag cggcaggcag attttccagc cgaaggacat tacccggtca taacgcggac
     8281 gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga
     8341 accagatgaa tggcggtaac aacgggcctt gccagccacc gaagaacagc gtcaccatca
     8401 atgcagagat ggtcacaatc ccgatgtact cacccacgaa gaacagaccg aacttcatac
     8461 cggaatattc aatgtggtaa ccatccgcca gttcctgctc ggcttccggc tggtcaaacg
     8521 ggtgacggtg acacaccgcc acgcccgcga tggcaaaggt aataaaacca aagaattgcg
     8581 ggatgacgtt ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg
     8641 cctgcgccac cacgcccatc agggaaagcc cgaggaacac ttcgtagctc agggtctgcg
     8701 cagaagcacg catcgcaccc agcagcgagt atttgttgtt acttgaccag cccgcaaaca
     8761 gcaccgcgta aaccgccaga cctgccatca tcaggaagaa caaaatcccg atgttcaggt
     8821 cggcaaccac ccaacccgga ctgactggca caattgcaaa ggccagcagc agcgaggtaa
     8881 aggcaatcat cggtgccagg gtaaagatga cgcgatccga gaatttcggg atccagtctt
     8941 ctttaaagaa cattttgatc atgtccgcaa ccagctggag cgaaccgccc cagccaacac
     9001 ggttaggtcc ataacggttc tggaacagac ccagcaggcg acgttcgcca aagctcatga
     9061 atgccccaca ggtgacaacc accagcagga tcaccaccgc tttgaggatg gtcagcagga
     9121 tctcaatcag ttccggtgat atccaactca ttgttgtgcc tccttgagat cctcaagatg
     9181 cgcgccagcc agcaccggag caatgccgga catacccatc ggcaagccca cctgccctgc
     9241 cgtcagtcct tcggcgattt caaccggcag cgtgaccgtg ttgccatcgt aactaaagga
     9301 gacgcgtgta cctgcgttca cacccaactt cgcggcatcc gctgggttga gtttgatgta
     9361 cggctgcggc atacggctct ggaagaccgg agcacgctgt gacaattcat cgctgccaaa
     9421 caggtggtag tacggcgcga tacgccattt cccgtcctgc ggctggaagc gtgccggtac
     9481 gctggtgaag taatccagac cgttttcgct ggtttcaaac agacgcacgc ccggatcgcc
     9541 aaagcgcagt ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg gggagttcca
     9601 gcccggcgcc caggcaaacg gcacttgcga acggtgcgcg gtcggctggt tgttaccttc
     9661 catcgagaag gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat gaacgctgat
     9721 attggcgcgc atggcggtac gaccgctgta acggtgcggt tcacgggcca gtttctgccc
     9781 acgaatacgg aatgtcgcat ccggcgcagc atctttgata cccgccagtt ccgggatttt
     9841 cgccacaaca gcgtcaatta catggtcgag ctgcgtccag tccacttcac ggctcagcaa
     9901 ggtgctgtgc agcgagtgca gccagcgcca gctttccagc atgacagttt tgctgtcgta
     9961 ataagccgga tcgtaaacct ggaagaaacg ttgggcgcga ccttcattat taattaccgt
    10021 accgtcgctt tcagcaaagc tggcagagga aagcaccaga tgggcgtttt ccatgatcgc
    10081 tgtgcgttga tgatcaacca ccatcaccag cggcgcttta gccagcgcag cattcacgcg
    10141 ggtagcagaa gcgtaacgat gcagatcgtt ttccaacacc accaccgcgt cggcgcgtcc
    10201 ggtttccagt tcggttaacg cttcttcaag cgaaccgcca cccataatgc ccagccccat
    10261 gctgttgacg gaacgggcaa tcatggtgat accaacgtca gcgccgcgac ctttcagggc
    10321 tttcgcgacg ttagccgcag cctgaatcac ctctatgcta ccggcgttcg tcccggagat
    10381 aatcaacggt ttcttcgcac ctgccagtgc ctgcacgatg acgtcgattt tgttttgcag
    10441 ctcaggttcg ataccgtcaa ccgctggtgc ggagttatcc agcgcatggg cgatggcaaa
    10501 acctaaacgc gcctgatctt caaccggtgc gcggtaagtc cacgccgcga tatcatccag
    10561 acgagtgtca tcaacgttgg taacaaacag cggatgcttc gcacgttgac cgatgttgag
    10621 gattgccgca atctgccagt cagccacttt ctgtgctgca gccatttcgc gcgctttacc
    10681 tttcaccgcc tgacgcactg ccagtgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc
    10741 cagcaccagt accgcatcgt aagattcgat ttcgcgcaga gccggagtat aaatgccgcc
    10801 ttcacgcagc actttcagcg ccagttgcag acgttcctgc tcaccgtgag cgataccggt
    10861 gtagaagttt tcttcgccca ccagttcacg cagcgcaaag ttgctttcca cgctggcacg
    10921 cggagaacca ataccgatca ctttcttcga ctgacgcaga atatctgccg cgccctgcat
    10981 tgcctgttcg gcgttgaggg taatgaaatc atcgccacga cgctgtactg gctgacgcgg
    11041 acgatccttc aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg
    11101 gtttaccgta ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac gttcaccggg
    11161 gctgatgtta cagccgatgg aacattgctg gcagatgctc ggcgcaaact gcatatccca
    11221 tttacggttg taacgctcgg agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc
    11281 gaccaggtta ccggaaaatt cgctttccag cgtgccgtct tccgggcgac cgaagtagac
    11341 gttgtcgtgc gcgccataaa cgcccagatc tgtaccgtca gcgtaatctt tgtagtaacg
    11401 cacacagcgg tagcaggcga tgcagcggtt catttcgtga gagatgaatg gccccaaatc
    11461 ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac
    11521 ggtcatatcc tgaagatggc agttaccgcc ttcttcacat accggacagt cgtgcgggtg
    11581 gttggtcatt aaccactcga ccacactttc acggaactgt ttcgcttctt cgtcgtcaat
    11641 ggaaataaag gtgccatcgg aagccggtgt catacaggac atcaccaggc gaccacgcgt
    11701 gtcttccgcg ttttggtatt gcttcaccgc acactggcgg caagcaccga cgcttcccag
    11761 cgccggatgc cagcaaaagt aaggaatatc aaggcccaga gacagacaag cttccagcag
    11821 gttgtccgct ccgttgacct cgtattcttt gccgtctaca tgaattgtag ccattagcat
    11881 gcttccagtt ttctcggtca gggaccgagc gttaatcgaa attcggttac cagcgctctt
    11941 tcagcaggtt cggctgaatc ccattaatca aatgggtatt gctgaacggc tgtttgattc
    12001 ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg taacggctcc actgcaccag
    12061 gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg acacagttgc tcaagtgttt
    12121 cgatatcgcc cggctggcct tcgccacgct ccagcgcacg cagaattttc acgctccacg
    12181 gcagaccgtc gcggcacggc gtacaccagc cgcaggactc acgggcgaaa aactcttcca
    12241 ggttacgcac cagcgacacc atgttgatct catggtcaac cgccatcgcc agcgccgtac
    12301 ccagacggct gcccgcttta ccgatacttt cgaattccat cggcagatca aggtgcgctt
    12361 cggtcaggaa gtcagtcccc gcgccgcctg gctgccaggc tttaaatttc agaccatcac
    12421 gcataccacc ggcgtaatct tcgaggatct cgcgtgcggt ggtgccgaac ggcagttccc
    12481 acagtcccgg atttttcacc cgaccggaga agcccatcag cttggtgcca gcatctttac
    12541 ttttcgagat gttctgatac cactccacgc cgttagcgag gatcgccgga acgttacaca
    12601 gggtttcgac gttgttgaca caggtcggtt tgccccatac gccggaggtt gccgggaagg
    12661 ggggtttcga gcgtgggtta gcacgacgcc cttccaggga gttgattaac gctgtttctt
    12721 ccccgcagat gtagcgccct gccccggtat ggacgaacag ttcgaagtcg aaacctgttc
    12781 ccataatgtt tttgccaagc aagcccgctt cagtggcttc ggcaatggcg cggcgcagat
    12841 ttactgccgc ttcgatatat tcgccacgca ggaagatgta gccacggtaa gctttcagcg
    12901 caaacgcgga gatgagcata ccttccacca gcaggtgcgg cagttgctcc atcaacaggc
    12961 ggtctttata ggtgcccggc tccatttcat cggcgttaca cagcaggtaa cggatgttca
    13021 tggattcgtc tttcggcatc aggctccact tcaggccagt cgaaaagccc gcgccgccgc
    13081 gccctttcag accagcgtct tttacctgat taacgatttc gtccggagac agcccggtca
    13141 gcgccttacg cgccccttcg taaccgtttt tgctgcggta ttcgtccagc cacactggct
    13201 gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg ataatgtttt
    13261 tcatttatac cgctccagca gttcagggat cgcttccggg gtcagatgcg cgtgagtgtc
    13321 ctcatcgatc atcatgtttg gccctttatc acagttcccc aggcagcaag ttggcagcag
    13381 cgtaaagcgg ccatcaaatg tcgtttgccc tggtttgatg ttcagctttt tctcgagcgc
    13441 cgcctgaata ccctgatacc cgttgatatg acagaccacg ctgtcgcaat aacggatcac
    13501 atggcgacca accggctggc ggaagatctg actgtagaac gttgccacac cttcgacgtc
    13561 gcttgccgga atacccagca catcggcgat cgcgtggatc gcaccatccg gcacccagcc
    13621 acgctgcttc tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg
    13681 gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc agctcaaaag cctcggtttg
    13741 tggttgttga ttctcgtgca taattagcgg tccacatctg acataacaaa atcgatactg
    13801 cccagataaa caatcaggtc agacaccagg ctgccgcgga tcgccgccgg aatttgctgc
    13861 aaatgcgcat aactcggcgt gcggatacga gtgcgataac tcatggtgct accgtcgctg
    13921 gtcaggtagt aactgttgat ccccttggtc gcctcaatca tctggaaaga ttcattggca
    13981 ggcatcaccg gcccccacga cacttgcagg aagtgggtga tcagggtttc gatatgttgc
    14041 agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc
    14101 ggcatgttgt tgaggcactg ctcaagaatg cgcagactct ggcgcagctc ttccactttc
    14161 agcattacgc gggtgtagca gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag
    14221 ttttcatagc cagaataagg acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc
    14281 aggcccgcgc cagtggtgcc ccactccagc gcctctttcg cgccataggc ggcaacgccc
    14341 tgggaacgac ctttcagaat ggtattttgc agcgccgctt tctcgtaaga cgccagacgt
    14401 ttcggcatcc agtcgaggaa ctcacgcagc agacgatccc agccgcgcgg caggtcgtgc
    14461 gctacgccgc caatacggaa ccacgccggg tgcatacgga aacccgtgat cgcttccacc
    14521 agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg
    14581 tcctgaataa aggtcgagat gtacagcagg tggctgttga tacggaacag ttcggagagc
    14641 ataacgcgaa tgacgttaac gcgatccggc acggtgatcc cggccagttt ctctaccgcc
    14701 agcacgtaag gcatttcgtt aacgcagccg ccgaggtatt caatacggtc ggtatacgga
    14761 atgtagctgt gccaggactg gcgctcgccc attttctccg caccacggtg gtggtagccg
    14821 atgtctggta cgcagtcgac aatctcttcg ccgtcgagtt gtaaaacgat acggaaagcc
    14881 ccgtgcgccg acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc gttttcggtg
    14941 ccgcgcttca tcccccactc ttccggtttg aaggtcaggg cttccatctc cagatcctgt
    15001 ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc gcgccgggta atctttacgc
    15061 agcgggtgac ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg
    15121 aaagtaatgc caaacagatc ccaggtttca cgctcatacc agttagcgtt cgggaacagt
    15181 ttggtgaagg tcggtacgtg caggtcgttt tctgccagcg ccaccttcag catgatgtcg
    15241 cggttacgat cgatagaaat cagatggtag aaaacggaaa aatccgcggc aggtaaccct
    15301 tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt
    15361 ttcggcagtt tctttaagaa atcgccaact tccagtaatt gttcacgctt gatccacaca
    15421 acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc
    15481 agttcgccaa ttaccggatc atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg
    15541 gttaagtcgg tcatattgtt caccattgca aatggtccgt ggtgactgtc ggcgcagtaa
    15601 gcttcgcgaa atagatttag gaataagcga agaaaaatcc ctttgccgac aggcgcaaat
    15661 taaatctcgt caggtgtacg caggttggtt acggcaatgc gttcaccgcg cttgcgttcg
    15721 cgctctgatt gcatattggc gcgataaacg ccctgatcgc caaccaccca ggagagcgga
    15781 cgacgttctt tgccgataga ttcctgcaac agcatcagtg cctgcatgta cgcttcagga
    15841 cgcggcgggc agcccgggat atacacatca accgggatga atttatcgac gccctgcaca
    15901 acggaataaa tatcgtacat accaccagag ttggcacagg cacccattga gataacccat
    15961 tttggttcca gcatctggtc atacagacgc tgaataaccg gtgccatttt ggtaaagcag
    16021 gttcctgcaa ccaccatcag gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca
    16081 aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta acagcaggaa
    16141 agaccgaagt tatacggcca aattgagttt ttacgacccc agttaaccat gtcattgagc
    16201 ttgcccataa acacgttttt gttaacttct tgctccagag ggtcggttac gatctcctgc
    16261 ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag cgtataatcc
    16321 atcttaatgc ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc cgggttcata
    16381 cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac cagataaacc
    16441 agacctgcca gtaacacaaa aataaaaatt gcagcttcca caaagcctac ccagccgctt
    16501 tcgcggatag aggttgacca tgcgaacaga tacagcgctt caacgtcgaa gataacgaag
    16561 aacatggcca ccagataaaa cttggcggac aggcgtaagc gggcggagcc gaccgagtcg
    16621 ataccggatt caaacggcac gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa
    16681 ccgcctacca gcatcaggca acacaggcca atggcaacga taagaaagat agcgaatgcc
    16741 cagtgatgag cgatgacttc agtggatgtt gacatactca ttgcttactc atcaaaagta
    16801 gcgccagatt cactgctctt cacggcagat ggacgccaca tcgattcatg gggaggaata
    16861 aaaaaaacct tacaataact gtagaaattc ttttatacag ctaattgatg tggtctttta
    16921 ctcctttcta taaccttttg tcaactttaa caaaagcttc ttcacattag tttacataat
    16981 atcaacacca ttagcattta atgccctttc accccagatc cttgacgact ccaggataat
    17041 tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac
    17101 aggaatttgt gtcgattcct ccccccaaaa gagagtattt tcttgatctg tgacacgctt
    17161 ttgttattcc ataacaaaaa cgcagcaaca aatttacgta ttttttaaca tcattgtagc
    17221 aggtgatttt tttcaggcga ttatttgtgc attcgggacg tgaatctctg gtgattgaaa
    17281 aataaacagt tttgtacgtt ctgcactatg cgaggaagga tttttactaa aaaaaagccg
    17341 ctggggttta aaacaccccc agcggctcgt tttttacact attgtctcag gaattatcta
    17401 tcgtccgtcg attactcgat atccctttca atcaacaatg aatcatcccc ttccggagca
    17461 gacattgggc tgtattgcca cggattatgg tagctctcca tcgcctgata gatcacctgg
    17521 gccagctcat tattactgga cgggtcatag cacagcaaat attcggtatc aggtagcggc
    17581 ggtaagccat ctactccact cagcacacgc aggtccgggc tcatcatctc aaccggcctt
    17641 gccgtcacgc caagaccggc tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca
    17701 taagccagtc gccatggaat atctacttta ttaagcgtcg ccagcaccat atcgcgaaac
    17761 ggacttggat catccagcaa tacaagaggg atcggctcgc ctttttgcaa tacgtattcc
    17821 gctgcgcagt accagtgtgt tggcgaggta cgcaggttca atgccttaaa cgctgaagga
    17881 cgatgggtgg ttaccatcaa atccacttcc tgggattcca gcatttctgc catataggca
    17941 ttacgtttaa cgcggacatc cagcgctaat ttcggataaa ccgaactcac gcgatttaac
    18001 aggaaaggta agatcgtatc ggcagattca tctgaagcac cgatagttaa cacgccctga
    18061 agattactga acattaatga tgagcaggcc tcatcattaa aacgcaggat tttcctggcg
    18121 taaccaagaa gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac
    18181 agttctttcc caacgagttg ttccagacgc tgcatttgct gacttacggc ggactgagta
    18241 cgacacacag cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt
    18301 ctcagcagat cgaggtcgag gttaattatc ggacgatttg cacttatcat atattatcac
    18361 ttactggcgg ctcatactga gctggttaat gctgtgcaca cacaaacaag caattccatt
    18421 tgtaatgtgc ctccctggca gtttcatccg gaacccggac gaaagtaaaa atgcatatga
    18481 gttgcactaa aaaagcgact cacattgttc cgttataatg cctgaagtag atcacagaat
    18541 atatcttcag ggatcgcata tctattaagt tactcactct tttctattta tgacatgcgc
    18601 gtgtttgtat aaatgtaaat gtgagtcctt gttccactct cgtgcagcat cgctggtcat
    18661 acgcgaacac gtacaaacag caatggtgaa gcggcatcaa gagcagggat ccgtcattta
    18721 tgcgagcatt ttaccccaaa aaccttttat ttataagggt cattgcgaat tatctgatgc
    18781 aaagttatgt tatgttaggc aaagtaatct tctattatta ataagcacat caaaaccttt
    18841 ttgaatatta aataataatt aattagcatc atcctcattc attaattccg cttaacaata
    18901 gtttcacacc ttacccccat tgcgacacct cggaaatcat cacgctgtta aactctccgt
    18961 aatgcggaaa tatcatcatc gcgtttcatt agtgaattct taaatgaggc attttcacac
    19021 aattatctta cagataaaaa aaccagactt gcaattaaga attagaacaa ttcactatat
    19081 aacattgcat gtaaagcata tacacctcat tattttgtca ttattaagtt attaacagca
    19141 caatcgagcc ttcccctctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg
    19201 gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg
    19261 tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc
    19321 cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa
    19381 ctgaacatcg gcaacccagc cccattcggt tttgacgcgc cagatgaaat cctcgttgac
    19441 gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaagggct ttactccgcg
    19501 cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc gtgatgttac cgtggaagat
    19561 atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac
    19621 agcggggacg aaatgttggt tcctgcacca gattacccac tctggaccgc ggcggtttcg
    19681 ctttccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac
    19741 ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggattgttat tatcaaccca
    19801 aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca
    19861 cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct ctacgacgat
    19921 gctgagcatc actcaattgc gccgctggca cctgacctgc tgaccattac ctttaacgga
    19981 ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg
    20041 aaaaaacacg ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt
    20101 gctaacgttc ctgcgcaaca cgccattcag accgcgctgg gtggttatca gagcatcagt
    20161 gaatttatta cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaactgatc
    20221 aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa
    20281 atcgacgcca aacgctttaa cattcatgac gatcagaaaa tggtgctgga tttcctgttg
    20341 caggaaaaag ttctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc
    20401 cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt
    20461 ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca
    20521 gtgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga
    20581 gccatttttt tgcccatctc tcccgcctga aactcattaa ccgctggccg ctaatgcgca
    20641 acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc
    20701 tggcagctat caaaaatcgg aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac
    20761 tggcgatgta ccacgatgcc tcagaagtgc tcaccggcga tctccctact ccggtgaaat
    20821 acttcaatgc gcaaatcgct caggagtaca aggctattga aaaaatcgct cagcagaaac
    20881 tggtcgatat ggttccggaa gaactgcggg atatctttgc gccgttaatt gacgagcatg
    20941 catatagcga tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt gcatatctga
    21001 aatgtctgga agaactcgcg gccggaaata atgaattctt gctggcaaaa acgcgactgg
    21061 aagcgacgct tgaagcgcgt cgcagccagg agatggacta cttcatggaa gtatttgttc
    21121 ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa gcagccggag
    21181 tctgcgtcgc atcagtcaat aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa
    21241 cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccaa
    21301 cttcacaaag tcactgaagc tgtaatttcc cggacctaaa accagggtgt taacaggtga
    21361 agacaccggt gtcataaagg cggcggatgc tgccatcgcc acgaccatcg caaatggata
    21421 aggcgacact cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt
    21481 cgcggtatta gagataaaca gcccaataac cgccgacaag acaaacaaac accccagcat
    21541 catatgtggc ccataaccgc cgccaatatc catcagccct ttcaccgcca gcgcgacacc
    21601 tcccgttttc tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg
    21661 ccagtgaatg gatttatagg cgctttcagc atctatacag cggaatttcc ccatcagcag
    21721 gcaggcgatg atagcggcaa caggattagg aatttcattt gtcagcatta acgccaccat
    21781 tagcaccaga cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg cttcactcac
    21841 ctcttccggt aagttcagcg ctacgaagtc gcggccctgt ttggccagca taccgatcag
    21901 tttccagtta cccacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga
    21961 accttccagc gccacgccat tgcgcttgag ccccaccaca ttcagtccgt agcgggtacg
    22021 aaaaccaatt tcgcgcaccg atttaccaat cagttctgac tcaggaatta atgaaatctc
    22081 tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc
    22141 cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag
    22201 caaaacgtca cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa
    22261 acgccgccag cgttcaacac cgatgacgtt agcgccataa cgctcacgta atttgagatc
    22321 atccagccgt tgacctatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc
    22381 ggtcagtcga tattcacgga taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg
    22441 cggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag
    22501 caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc
    22561 acgcagcaat tcactgttga ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc
    22621 gctaatcagc ccggcaaaac ttaacggcat catcagacgt gacggcgaag tttgcatacg
    22681 catggcaacg cttaacacca cgggaataaa gatagcgaca acgctggttg aactcataaa
    22741 cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc
    22801 cgccactttg accagccatg ttcccattac ggtggcaaca ccggtacgga ccaaaccatc
    22861 gccaataata aacaaggcgg caatcaggac aacgttagga tcagaaaagc cggaaaatac
    22921 ttcggggacc gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc
    22981 atccatacgc actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac
    23041 ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggggatga cttgattctg
    23101 ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata
    23161 acatcgccat ttggctgttt ggtcacagta atttgctcca gactgtggag gaccaaatcg
    23221 acctcattca ggcgcggggt atctgccgga gcgttaaccg caatgacatg gcaacccgcc
    23281 gccaggccag aaagcacgcc agcgggagca tcttccacca ccacacactc ctgcggcgca
    23341 agccccagca gctgcgcacc taacagatac gcatcaggtt ctggttttcc gcgcttcact
    23401 cgctcagcgg ttacaaacac ctctggtgcg ggaagccccg ctattttatg gcgcgctcgc
    23461 gctaccggca tggagccaga agtcacaatg gcccacggaa tacctgcttt attcaaatga
    23521 ttgagtaagg cgattgcccc aggaagcgcg gtaatacctt cggtttccgt ggcctcgatt
    23581 tgctccagac gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg
    23641 cgcaaagagg tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggcgctaac
    23701 ccatgacgtc tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc
    23761 gttccatcaa gatcaaacag aaaacctttg caccgcacgc gggcctcctc aggcgttgat
    23821 aatttgatta atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt
    23881 aagcatgcgc tgatattttt cccacattgg cgtctgggcg ttaaaaccat gagtcccagc
    23941 gtcaaaatgg gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg
    24001 tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg
    24061 gtcctgcaaa ttagaccagg aaacatacaa cgcatgatac atctccataa tgtcgatgat
    24121 agtgcggcag gtttcttctt tcagctcgcc aaactcgcga tccaattcac gcatctgtaa
    24181 tccgtaacca cgctcaataa ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc
    24241 gagcatagtc atcatcttgt actgattaga caaaataaga cgttgcgcgt tggtcatttc
    24301 cattgttgac tcctgtatca ctctactacg gtgaaaaaaa agaaggctga gtatgccttc
    24361 ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt
    24421 tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg
    24481 ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttaaggattg
    24541 cagggggtaa tggcgttaca gaacgcttgc ccagccacca caatccctgc atgggtaaac
    24601 ttaaggcgaa cagtgcagtg gcaactgccg gcccaagcag accgcccagg gcaatctgcc
    24661 agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga
    24721 taacgcgatt ttcgacaaag accggggcaa ggcgtttttc cagtggccac gtctttgagt
    24781 aatgctgtcc gcggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca
    24841 tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg
    24901 ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg
    24961 aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct
    25021 gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg
    25081 cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct
    25141 gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg
    25201 ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag
    25261 agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa
    25321 tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca
    25381 actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta
    25441 tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt
    25501 ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc
    25561 tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg
    25621 tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt
    25681 acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct
    25741 atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca
    25801 cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca
    25861 cctctatggg tctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg
    25921 atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca
    25981 aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct
    26041 atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc
    26101 accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg
    26161 ttgcattcac tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca
    26221 aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca
    26281 aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag
    26341 aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag
    26401 ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc
    26461 ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg
    26521 cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca
    26581 gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc
    26641 caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa
    26701 tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc
    26761 tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct
    26821 gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac
    26881 tgacaccccg gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggtggtgc
    26941 caaaaacacc aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca
    27001 gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa
    27061 caatgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc
    27121 gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac
    27181 catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg
    27241 cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga
    27301 ccgtcctgac gtgctggtgg ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc
    27361 cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc
    27421 tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga cctctctgag
    27481 cctgcagagc ttcaacctgg aagttccggt tgacgatcac gaacgtatcg agaaagttca
    27541 ggaatacgtt gctaactaca tcaacgctga ctggatcgaa tctctgactg ccacttctga
    27601 gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac tggcgcgcaa
    27661 agcgggcaaa cgtatcgttc tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc
    27721 tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa
    27781 ccgtgttgca gcgtctcagg gtgtagaact aggtgcaggc attgaaatcg ttgatccaga
    27841 agtggttcgc gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca aaggcatgac
    27901 cgaaaccgtt gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga
    27961 acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg
    28021 tccgccgctg caactgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt
    28081 catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac
    28141 cgctgaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat
    28201 cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt
    28261 agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga
    28321 cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa
    28381 ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac
    28441 cacctacaaa gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg
    28501 tatgcgcaaa ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac
    28561 catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccacagct
    28621 ttgcgctgcg gatatctgaa ccggaaataa tcactatttc cggtttttta ttctcttaat
    28681 ttgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatc
    28741 tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat
    28801 cactgaatcc aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt
    28861 tgttgctatt ttaaccagtc ttgccacctg ggtagttccg gtggggatgt ttgacagtca
    28921 ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc
    28981 atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac
    29041 gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa cctcaggatc
    29101 gaaatacggg acagccgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg
    29161 cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg
    29221 cgggaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt
    29281 atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg
    29341 gctgggctat gacagtatta ccaccgtcct ggtgacttat attgccacgc aaatcggttt
    29401 tgccagttcg tggatgaacc cgttttgtgt ggttgttgct caggggattg ccggcgttcc
    29461 ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct
    29521 gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca
    29581 tgagtccgac cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt
    29641 tggtgactgg ctggtattga ttgtcctgac agccgtaatg gtctgggtga tttggggcgt
    29701 gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt
    29761 gattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc
    29821 atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa
    29881 agggattttg ctgctggttg gtaatggtga agcaggtaat gccagcgtgt taaatactat
    29941 cctcaacagc attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat
    30001 gttgctcttc caggctgtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcatt
    30061 aactatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt
    30121 gctggcgttc cagtttggtg atggcttcag ccacatcatt tacccaacct cagcttcgtt
    30181 aatggcaacg ctcggtgtgt gccgggtgga cttccgtaac tggctgaagg tgggcgcgac
    30241 cctgcttgga ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg
    30301 ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt
    30361 ctctacggct tcatttttgg cattgcgctt catccacaac gccagcgctt tcagcgagtc
    30421 tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaactccact
    30481 gacttcatct tcctgtaggg cgaagggacc gtgagagacg caactgaaca acgcgcccca
    30541 gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc
    30601 aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg
    30661 aactacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg
    30721 acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg
    30781 cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt
    30841 gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc
    30901 tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac
    30961 ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat
    31021 gccataaccc gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa
    31081 aatctccccc cgctgttctg ccactggcag atgggtatga ccgtacacca gcacatcgtt
    31141 ctggtttaaa gcaggtagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg
    31201 ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca gcatcatttg
    31261 atccacttcg ctgtcgcagt tgccacgcac agcgataacc ttatgtgcca cttcattaag
    31321 ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt
    31381 caacacgtcg ccaaggatca ccagccactg ggcaccgctt tggacaaaca actccacaac
    31441 acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca
    31501 ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt
    31561 gcttcgccga aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat
    31621 aatccaccag gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg
    31681 ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc agtgctaaca
    31741 aatgttctgc catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg
    31801 cttttttcgc ccccgcaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat
    31861 cgctgcgcag ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat
    31921 aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag
    31981 attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac
    32041 cttgccacgt cggctgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg
    32101 acaggccttt ttcctgcaac gccacccagg cggataacac ataaggggag aaaaagtggg
    32161 catctgacca aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg
    32221 gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc
    32281 gcgctttcta tacttgtggt gttcagatga ttaaacaacc ggagctgcaa tgatcgatct
    32341 ctatttcgcc ccgacaccca atggtcacaa aattacgctg tttctcgaag aagcagagct
    32401 tgattatcgc ttgattaagg tagacctggg gaaaggcggt cagtttcgtc cggaattttt
    32461 gcgcatttcg cccaacaaca aaattccggc aattgttgat cattctcctg ccgatggcgg
    32521 cgaaccgtta agcctctttg agtctggtgc cattttgttg tatctggctg agaaaacagg
    32581 actctttttg agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt tattctggca
    32641 ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca
    32701 aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtcttt accatgtact
    32761 aaacaagcgg ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat
    32821 tgcctgctgg ccgtgggtta atgcctggac tcgcctgcga attgacctcg caatgtatcc
    32881 ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct
    32941 aaaagcacaa ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta
    33001 tttcatccta agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt
    33061 ataaagaacc ttcgtttgcg tacgtttatc ggaattaagg aagaagaaat taacaaccgt
    33121 caggatattg ttatcaatgt gacgatccac taccccgccg ataaagcgcg cactagcgaa
    33181 gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag
    33241 aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa
    33301 catcactggg tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc
    33361 gattcggtat ccatgacctt aagctggcag cgttaatcgc catatcggga ggctgcatga
    33421 acatagtgat caccggaggg acgggattaa ttggtcgcca tttgattccg cgtttgctgg
    33481 agctgggcca tcaaattacg gtagtgacgc gtaacccgca gaaagccagt tccgttctcg
    33541 gccctcgggt gacactatgg caagggcttg ccgatcaaag caacctcaac ggcgttgatg
    33601 cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac aagcaaaaag
    33661 agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg attaatgcta
    33721 gcgacacgcc gccgtcggta ctcatttccg gctcggcaac gggctattat ggcgacttag
    33781 gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttactcat aaactctgcg
    33841 cccgctggga agagattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc
    33901 gaaccggcgt ggtactggca ccggacggcg gtattctcgg taaaatgctg ccaccgtttc
    33961 gtcttggcct gggcgggccg attggttccg gtcggcagta tctggcctgg attcatattg
    34021 atgatatggt caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata
    34081 tggtttcgcc ctacccggta cgcaatgaac aatttgccca tgcgctcggt catgcgctgc
    34141 atcgcccggc tattttgcgc gtccccacaa ccgccattcg gctgttaatg ggcgaatctt
    34201 cagtactggt attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg
    34261 cgtttcgctg gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac
    34321 agcaaacatc cgccagttaa ctccctgtgt tacaggatta gtggctttgc gcgataagat
    34381 cgtctggtga aagtcgggtc accatcataa ctaactctct gtctaaacct ctatccagca
    34441 tctcctgagc aatacgcagg gcttcttcgt gtttgccctg cattgcgcct tcttcacgta
    34501 atctgtcagc aatggtcatc agtttctcct tttcttgtgg tgcgcgttct gttatctcac
    34561 caataaatgc acggaaacgc tgggcatccc ctgtttgtaa tacgtaatta aacagggctt
    34621 ttagctgtct gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc
    34681 ccaacagatc gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcggt
    34741 gttgcataat ctcgtcatcc ggcaccacgg taatatccac caacggaaac gctgatgaat
    34801 atattttgcg ggctatagca ggctcggcaa attcatcaag ccagcagagt gaataaggat
    34861 aaggacttct gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc
    34921 ccgcatcaag atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta
    34981 attcttccgg cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg
    35041 ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt
    35101 ttggttctag tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa
    35161 tattaataaa attccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat
    35221 gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac
    35281 gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa
    35341 ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat
    35401 tccccttgaa aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc
    35461 ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc
    35521 tcttcttcta ttttcccctg atggaggaaa atgacgtgag tagaaacatg gcgagcaaag
    35581 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata
    35641 cgtaacactt cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa
    35701 acttctggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa
    35761 agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc cgactttcgc cagatacttc
    35821 accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg
    35881 acgttttcca acaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg
    35941 cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg
    36001 cgcaccagat tgatcgtctg gccattgacc acgatcgatc cttcactcgg tttttcgagg
    36061 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt
    36121 acatctccgg cattcgcttg cagtgagacc cctttcagca cttcgtgttc gccatagcgt
    36181 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
    36241 aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata
    36301 agagatgatt aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc
    36361 ggcgttaata tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc
    36421 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
    36481 agaaggcaaa ataatgcagc gatacatttt aaaggtcgag aagccatagg ctctggcggc
    36541 ttcaatttcc ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc
    36601 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt
    36661 aaggaattcg gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa
    36721 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
    36781 gtatttatta ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac
    36841 taccgacaaa ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt
    36901 ccacagcagc ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca
    36961 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacggt ggtgaaaacc
    37021 aggtaaatca cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc
    37081 agctgcgtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
    37141 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
    37201 atgatccgcc gaaacacttg cccgcgagta aaaccgaacg ccgtcgccgc ctctatatgc
    37261 cctttcggca ctgccataaa cgcgccacgg aacgtttcgg taaaataagc accgtagata
    37321 aaaccgagag tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc
    37381 gcctccgtca ccatgtttag cgcaatctgc aaaccataga aaatcagcag catcaacacc
    37441 aagtccggca cgcctcgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga
    37501 ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag
    37561 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac
    37621 aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
    37681 ccgaagggag cgggctttct ttcaccgatg gcgattaacc accataaaca tcaaaatcga
    37741 agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt
    37801 tcagtgcctc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa
    37861 acagtttttc atctttaaca gacggaccac cgaatttgta atctttaccg acgggttgtt
    37921 tgaggaaacc ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag
    37981 tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg
    38041 cccagtgttc attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
    38101 ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc agacgagaat
    38161 cggcagcgta cagtttgtcg gtgaaggcaa tttcttgctg acgtttttcc gtaatggaaa
    38221 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg
    38281 ggttttcgac aaacgtacat tgcgtattga tgcgcttgca caactccttc gccagctcga
    38341 tatcgaaacc aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt
    38401 cggtaccgat gcggatgttt tgcggaattg cagcaagtgc cgcagttgcg ctggagaagg
    38461 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
    38521 ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
    38581 aaaatatccc gccccgttag acgtattatt tttcattaaa aatgcttaag tatgtatttc
    38641 aatgcaccac gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac
    38701 agcttcccag cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc
    38761 ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt
    38821 tcagcatcat ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt
    38881 actgatgagc cagcaaaagc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg
    38941 gcagcaactt catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc
    39001 aaatcctggt tggcataggc caccacatcc acgcctttac tacgccaggt ctcgttagcg
    39061 taagcttcct gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
    39121 gttggctgaa tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg
    39181 tcggagaagg caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatagcg
    39241 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta
    39301 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa
    39361 tcacctttag catctttcga cgagaacagc gcataggtgg tatcggttcc gatacgtacc
    39421 gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa taaagacaga
    39481 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta
    39541 ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacaacagt
    39601 aacattcaac gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag
    39661 caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat
    39721 ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca
    39781 gcgggcaaag agatcttcag gaagggttat tgcaaactgg tcaagaacac gattaaccgt
    39841 ctgatttatc acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat
    39901 aatcaccgca ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg
    39961 tgtttcacgc acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg
    40021 ggtcagtaag ccatcagtat agctatggac aatgccggac agggttttga ttgaacaggg
    40081 taaaatcacc atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg
    40141 cgcatcgagc gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag
    40201 ggataaggtc tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc
    40261 gcgcagaacc tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac
    40321 aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc
    40381 ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt
    40441 tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac
    40501 gtaacgtatc gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt
    40561 tgaacaccga gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca
    40621 gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa
    40681 cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg
    40741 ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg
    40801 ccatctcgat aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga
    40861 cgtttttatc gcggaactcg gcgcggttgg cattcagttt acggcgcacg gacttacgac
    40921 gcagctgctg gcccggcatg ataaaggtgc ggccaacata gcggttttta acgaagccct
    40981 ggcggtacgg tttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttctg
    41041 ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca
    41101 gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgata aacgagtccg
    41161 ggcgggcaaa gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt
    41221 gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac
    41281 gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg
    41341 tacggttctc gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc
    41401 ggaaagcaac cataccgtgg ccgataatca tcgccacaca ggcatacgcg ccgcggatta
    41461 agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga
    41521 agttgtccag ctcgctggcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga
    41581 tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc
    41641 cgttgtgggc aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg
    41701 cgctggagct gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac
    41761 gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga
    41821 agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca
    41881 ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac
    41941 cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga
    42001 aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg aattgcggga
    42061 tcagctgtga tttgctccag tcttcacttt tcgacacccc ggtaaaggag tcgagaaaga
    42121 agagaatggc ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc
    42181 gatcggtgcc tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgatag
    42241 caccaacgat cagggtagcg ataaacagta ccgcgatggc aatcccattt cgaaccagtt
    42301 cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga
    42361 aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgtgg atcaggctaa
    42421 ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc
    42481 cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt
    42541 gcgtagggat ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca
    42601 tcgacttttt atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt
    42661 atccgaccta cggttctaac actgcccaag cctgataaga cgcgccagcg tcgcatcagg
    42721 caagactgta ttaattcggc gtatagccca ttaccacgcc acttaagcca gaaagttgct
    42781 tcaactcacc cagcgaacct ttcagcttat ctttcgaggc atccggccca accagaatac
    42841 gggtaatttt accctgcact ggcgtggatg gcgacgtata aacccgataa ccggcaccgc
    42901 gcagcttacc gacaatctca ttcactttat cggcattttt cagcgcaccc agttgcacaa
    42961 cataggcttt acccgttggc gctgcttttt cttccacgac cggcttcggt tctggcttcg
    43021 gcgcaggtgg tgcttcaacc tttggtttag gcggctccac cggtttcggc ttcggtgggg
    43081 cgaccggtgc aggttccggt tcaaactcgg tgttattggc tgcaatagtg gccggatcga
    43141 gcgacggtgc tgcggcatca cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg
    43201 tcggcaacgc ctgggtggcg gctggcatca tatcaggctc atcacgatcg cccgctttcg
    43261 gcaccagcgg gatagccgcg aactcatcct gataatgttt tttctgcccg tccagcagcc
    43321 ctggaagtac aatcaccccc agcgccacca gcacgatcgt gcccactaac cgattctgaa
    43381 acttacttgc caccgcttct cctcgcgtca atcacttcca tgacatgtgc gaccgtgtgg
    43441 aaagaaccac acaccagcac ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat
    43501 gcctgcgcaa cgctatcaaa tgatttgccg ttacccaaat gctcaagcag ttgttctgcc
    43561 gtggcaccgc gcggcccttc cagtggcgca caataccagt catcaaccac gcttttcaac
    43621 caggccagag ttccggcaat atctttatca tgtagcatac cgataaccgc cagcacgcgc
    43681 ccgtttttcg gtagcgcttt catacgcccg gtgagatatt ccgccgcatg tggattatgc
    43741 gcgacatcaa aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt
    43801 gcgctggcaa tcccgtcgcg aatggcattt tcactgactt ccagcccgct ggcacgcagt
    43861 gccgccagcg ccgttgcggc attcggttgc gggacaagcg gcaacggcag attttccagc
    43921 gtgccgtgag catcgctaaa cgcccagtca tgatcggtga cggaatagtt ccactcaacg
    43981 ccccgacgtt gtaacagtgc acctttttcc tgcgccacat cagcaatggt agaaggcatt
    44041 tccggctcac cgacaattgc cggtttttcg ctgcggaaga tgcctgcttt ctcgcgacca
    44101 atactttcgc gatctggacc cagccagtcg gtatgatcca gcgcaatact ggttactacc
    44161 gcgacatcgg catcgacaat attggttgcg tccagacgac cgcccagccc tacttccaga
    44221 atcaccacgt caagttgtgc ctgcttgaac agccacaacg ccgacagcgt accgtactcg
    44281 aaataggtca gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg
    44341 gccgattccg gcaattcctg gccctgcaca cgtacgcgct cggtataacg caccagatga
    44401 ggcgaactgt agacgcccac tttgtaccct gccgccatca gaatcgactc cagcgtacgg
    44461 caggtggtgc ctttgccatt cgtacccgca acggtaaaca caaatggcgc tggtttcagg
    44521 acgccaagac gcgccgcgac ctggctcacg cgctcaaggc cgagatcgat agttttactg
    44581 tgcaggtttt ccagataaga aagccacgaa gccagaggcg acgcggcttg aggagtgcgt
    44641 ttgataatca tggtatccgc tgattcgtta cggtgagaat agcaaaaggg cagagccagt
    44701 ggccctgccc ttatcagtta tcaggcctca ggttcctgat ccggtaccgg gggtaccact
    44761 acgccttcac gcggcgcttc aggattcggc gctggcagat tcatcaactt cgccagaatg
    44821 ctcgccagtt tcaggcgcat ttccggacga cggacgatca tgtcgatcgc gcctttctcg
    44881 atcaggaatt cactgcgctg gaatctaggc ggcagttttt cgcgaacggt ctgttcgata
    44941 acacgcggac cggcaaagcc gattaacgct ttaggttcag cgatgttgag atcgcccagc
    45001 atggcgaagc ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga gatgtacggc
    45061 aagccgcgct cctgcatttt tgccagtgcc gcagaggttt tcgccatctg catcagcgac
    45121 atcagtgctt cctgcatacg tgcgccacca gaggcggaga agcagatcag cgggcagtta
    45181 tcttcctgcg cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa
    45241 ccgcccataa aggcgaactc gaatgccgca gcgacaaccg gcattccata cagagtgcct
    45301 ttcatcacca ccagcgcatc tttttcgccg gtttctttct gcgcagatgc cagacggtct
    45361 ttatacttct tggagtcacg aaacttcagc tcatctttcg gctcaagctc gctacccagc
    45421 tccacaaggc ttccttcatc taacaggcta tgcaggcgat tacgcgctgt catacgcatg
    45481 tgatggtcac acttcggaca gacctcaaga ttacgttcca gctcagcgcg gtataaaacc
    45541 tgaccgcagc tatcacactt agtccacacc ccttcaggaa tgctcgcctt gcgggtggga
    45601 gtaatgttgc ttttaattcg ttcaatccag ctcattaggg acctttctgt ctgaacctgg
    45661 ttcgatgcca gttttatctt tggggacgca taatgccatt tttgccccca acagaccatg
    45721 aatgttgcac attaaaacat aacagcccga aactttggat aaaaaagtgg tcgaaccgcg
    45781 gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt gtgacggatt atttcgatga
    45841 cgcccggcaa aatagaaacc acaataatcc cgacgatcag cagcttaagg ttatcctgaa
    45901 ccatcgggat tgtaccgaag aaatagcctg cgtaggtaaa aagcagtacc cacaacagtg
    45961 cgccgatcac gttataagca gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa
    46021 acggggcgaa cgttctgacg atcggcacaa aacgggcgag aataatcgtt ttgccgccat
    46081 gtttctcata aaactgatgg gttttgtcga gataactgcg acggaaaatt ttcgaattgg
    46141 gattactgaa taacttttca ccgaacaacc gcccgatagt gtaattgacc gcgtcaccca
    46201 caatcgcggc gatcagcatc agcactacca tcatatggac gttgagatcg ttggtttcca
    46261 gtgatgccaa cgctccggca acaaacagca atgaatcacc cggtaaaaac ggcattacca
    46321 ccagaccggt ttcacagaac aaaattaaga acaaaatggc ataaacccag acgccgtact
    46381 ccgcgactag ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa
    46441 tcaggtccat atttgcctat gccttgtact cgtcatcttt caggctgtaa ctacgttggc
    46501 tacagcctga aatgttccga gcattattct cgttagtccg ccagaaatag cgggcccatt
    46561 ggcggttttg gaagatcata ccggtcaggg taatccaccg cgaccagata cagcccttcc
    46621 gcttttgccg ttgctgccgc cagcgttctg tcctttgccg ccagcaactc tgctatccag
    46681 ctctccggct ggttgtgggc accgacttcc atcaggctgc cgacaatatt cctgaccata
    46741 tgatgtacaa aggcattcgc tttgatatca accaccacat aaggaccgtg acgcgtgaca
    46801 ttaatatgca taacgttgcg ccacggggtt cgggactggc actgcaccgc acggaacgag
    46861 gtgaaatcat tctcgcccag caagcattgc gcagcccgat gcatccgttc agcgtccagc
    46921 ggttcgtaaa aatgggttac ccctttactc agtaccgccg ggcgcagccg atgattgtag
    46981 atgatgtagc gataacggcg agccgtggcg ctaaatcgtg catgaaaatc atcaggtaca
    47041 gctttaaccc aacgcacggc gatgtcacca ggtaaattcg catttacgcc caaggtccac
    47101 gccgcgtctt tgcgctgcgc ggttgtttcg aaatgcacca cttgcccggt accgtgtacc
    47161 cctgcgtcag tacgcccggc gcagaagacg gtgatgggtt cgttcgccac ctgggagagc
    47221 gccttttcca gcttctcctg cacactgcgg acttcattct gccgttgcca gccgtaatac
    47281 ttactgccgt cgtactcaat gcccagcgca attttataaa ctggcagttg ttgctggtcg
    47341 gacattagta cagatactcc tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc
    47401 cgccaaagcg aacgttatcg gccaccgacc agaactggac ttgctccggc ataccgtagt
    47461 cattacgcac gcagccaaca gaaagatgcg gcgtacccga agcatcccct acctgagtcg
    47521 ggaattcgtt ctcttcagag agcacaatat cttcgccctg agcaaacgca tcacgcgctt
    47581 cttctgccgc cagcggacgc agtgcttcaa agttgaccat ctgggcatga ccgtagaata
    47641 ccggtgcctg gacgacgctt gccgaaatca tcagcccttc gtcctgcagg attttgcgta
    47701 cttcgtcaac gatacgacgt tcttcacgca cgctaccttc gctatccggc aacaacggca
    47761 gcatattgaa cgccagttga cgaccgaaga aatcttcttc gtcaatcgga atgccgttga
    47821 gcaatttcgc actctgcccc gctaacgcat cgaccgcttt tttgccctgg gcggaggctg
    47881 aaatcaggct ggtaacactg atacgcgaca aaccgccctg atcgattaac ggtttcagtg
    47941 ccgccagcag ctggctggtc agactgtctg gtacggcgat gacattccgg ttgcggtaat
    48001 cggtcagtac aaacgggttt acttccggca ccaccagcgg tacgtcgggt tcgagagcaa
    48061 acagtccgct gctgtcgatc accaggcaac ctgagttggt cgcttcttca acccaggcag
    48121 cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag
    48181 cggcatcctg cacggtgatt gttttaccac caaagcgcag ttgttcgcct gcgctttcgt
    48241 tacgtgccag tgcataaatt tccccaaccg ggaactgacg ttcagccagc gtttcaagca
    48301 gggcttcgcc catagcgcca gttgcgccca ggacggcaat gttccagcct tcagacatgg
    48361 tggtttactc cagaaatagc aaagctccct gccaatgtta cagcagagag catgaagaag
    48421 agattaacgt gccggatgat gaacggcgtt aaaacccagt ttacacagca atgatgccgc
    48481 actggcgtca tcacaaatta catacagaga tgaccattca cggcgctcaa gatagttttt
    48541 gcgcagttta tcgaactcac ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg
    48601 gcgcacatca tacaccaaat gcaccagcct tttcagcgtc ggttgatcga gcggaccatg
    48661 cagcgtaatg cggccaaact caggcgcggg cagcaacgta tccagcgcaa cgtgttgttc
    48721 atgcccaata aacttgctat aggcttcaaa cacttgcgtc gtgccacgcg ctttaccctc
    48781 cagggtatag cctgcgatat gcggcgtgcc gatatccact tttgtcagca attccacatt
    48841 aagttccggc tcgccttccc agacatccag caccacgctt aacttctggc cttcattcag
    48901 gcaggtcagc agcgcggtat tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc
    48961 tccgggcttc aggctacgga tcagtttttc atccgccaga tgtagcgttt tgtacggacc
    49021 atctttaaag agtggcgtat ggaaagtcag aatatccgcg cgctggacta actcatccag
    49081 cgagcggaaa tcaccctcat ccccacggtc ggcgcgaggc ggatcgcaaa gtaaggtttt
    49141 aatccctaac gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat
    49201 ccccaccgta cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca gcagggagga
    49261 gaaaacatat tccaccaccg caatcgcatt acagccaggt gcagcggaaa aaccaattcc
    49321 cgcctgcttc aaccatgctt catcgacatg gtcggtcccc gcagtggcag ttccaacaaa
    49381 tttaatgggt tttcctgcca gcaaagattc attcactttc gtgaccgaac gcaccatcag
    49441 cgcatccgcg tctgccagtt gagcgacggg gattggacgc ccgggaaccg cgatcacctc
    49501 acccaaacgg ctaaataagt cgcgggcata aggcatattt tcatcaacaa ggattttcac
    49561 gtttgtgtta cctgtatgag acgagagtta accggacaag tatgccataa tctcgcggcc
    49621 aggcatactt gcgaagattt caggtataag gatacgtaat gatacaacct atttccggcc
    49681 ctcctcctgg gcagccacca ggtcagggag ataacctgcc gtctggcgcg ggcaatcagc
    49741 ctttatccag tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg acctcactga
    49801 cgcaacaaca aagagcagaa ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag
    49861 attcaccgct gctttcgcgt cacttccctg ccgctgagca taatctggcg caacgtctgc
    49921 tggccgcgca aaaaagccat tctgcccgcc agcttttagc gcaattaggg gagtatttac
    49981 gtctggggaa tcatcgtcag gcggtcacgg attatatccg tcataacttt ggtcagacgc
    50041 cgctgaatca gctctcaccg gagcaattaa aaaccattct caccctgttg caggaaggga
    50101 agatggttat tccgcaacca cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg
    50161 agcacaatgc gctaaaacag ctggtgacca aacttgcggc ggcaacgggg gaacccagca
    50221 aacagatctg gcaatcgatg ctggaacttt ccggggtgaa agatggcgag ttaattccag
    50281 cgaaactgtt taaccatctg gtgacctggc tacaggcgcg tcagacgcta agccagcaaa
    50341 atacgccgac gctggaatca ctacagatgg cgctaaaaca acctttagat gccagtgaac
    50401 tggcggcgtt atcggcatat atccagcaaa aatatggcct ttctgcgcaa tcatcgcttt
    50461 cttctgccca ggccgaggat attcttaatc agctttatca acggcgggtt aaagggattg
    50521 atccgcgtga tatgcaaccg ctgcttaatc cttttccacc gatgatggac acgttgcaaa
    50581 atatggcaac gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg atgctggtct
    50641 ggctggttcg ttaaccccga cgaaatgaca gtatcgtgac aataataccc agcaccgcag
    50701 agatcgcccc ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc attcccgcca
    50761 gcggcccgga gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc
    50821 cgcgaacttg tgaggggacg cgtttaacca cctccacgcc cagcgcagga aagataagcg
    50881 aacatccggc tccggttaac gccgcgcccg ctaatgcgac ccatgcacct ggggcttgcc
    50941 agagcagcaa caagcccacc gtttctacaa gcagagagac aatcgccact ttcacgccgc
    51001 caaaacggtc cggcatccag ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg
    51061 cggtaagagt aaagcccgcc atcgcccatc ctttgctggc aaagtagagc gaaacgaaag
    51121 tcccgataac cgcaaaacca acgccttgta gtgccagccc taaccctggt ttccagataa
    51181 gcccgacaac gctccacagc gatggacgtt ctcccgccag ggccggtact ttgcgcactg
    51241 tgccgttaca ggcccacgcc agtaagggta ataccattgt ggtgatcgcc agtgcggcaa
    51301 aaccgtaatg gctatgaatc aacaggccaa gcggcgcacc aacagcgagg gcaccgtaaa
    51361 tcgccattcc gttccatgac atcactttgc cagagtgttt tggccctacg atgcctaacc
    51421 cccaggtcag agcgcctgtc agtaactggc tttcaccaaa cccaagaatc aaacgcccga
    51481 tgaccaacag ggcaaatttg aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg
    51541 cgccgccagc cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt gcgccatatt
    51601 gatcggccag tcgcccggcg taaccacgcg tcagcaccgt agccagaaac tgaatcccga
    51661 cggcaatgcc gaccatggta ttgccatagc ccagatcatg atgaacaaac agcgggataa
    51721 ccggcaacgg caaccctacg gtcatgtagg tgagaaaaac cgcaaaagag atgcggaaga
    51781 gcgaaaaact ggcagaagat cgtgtttcgg tttggcttac agcagtcatg cattactcca
    51841 gaatgcagcg caaggcgagg agtatccccg tctcatctct ctggtttcag ggttacggtg
    51901 cgttggcagg atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg
    51961 cctagaactg gtggcgattg tcaatgatgt gaaaaaggga accatcaggt tcccttttgc
    52021 gttagtgccg gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc
    52081 gacctactcc ggcctactgc gaattaatct tttcagcttg cgcattacca gcgtggcgtt
    52141 ggtgccgccg aagccgaagc tgttagacat aacagtggtc agttcgcgat cggtcgtttc
    52201 ggtcacgatg ttcagacccg cagcctgctc gtccagctct tcaatgttga tgctcggggc
    52261 gataaagccg tgttccagca tcagcagaga gtagatagct tcctgcacac ccgcagcgcc
    52321 cagtgagtga ccggtcatgg ctttggttgc agaaatcgcc gggctcttat cgccgaacac
    52381 ttcacggata gctgccagct ctttcacgtc gccaaccgga gtcgaagtac cgtgagagtt
    52441 caggtaatcg attggggtat caacgccatg catcgccatc tgcatacaac gtactgcgcc
    52501 ttcgccagac ggagcaacca tgtctgcacc atcagaggtt gcgccgtagc caacgatttc
    52561 agcatagatg taagcaccac gcgccagcgc gtgttccagc tcttcaacca ctaccatacc
    52621 gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg taagtacggg aggctttttc
    52681 cggggtgtcg ttgtatttag tagatagcgc acccatcgcg tcgaattcgc aggccatttc
    52741 ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc tgtttgccca gttggatctg
    52801 ctctactgcg ttaccgatac agtgtgcgga agtcgcacac gcggagctga tggagtagtt
    52861 aacgccatga attttaaacg gcgtggcgag acaggcagaa acgccggatg ccatcgcttt
    52921 agtgaccaca tacgggccaa ccgctttcag gccgcgcggg ccgcgcattg cgtcagcgcc
    52981 gaacacctgg aaacgcgggg aaccgccgcc ggaacctgca atcaggccaa cgcgcgggtt
    53041 attctggtaa gcttccggag agaggcccgc atcagcgatt gcctgctcca tagaaaggaa
    53101 tgcataaatg gatgcgtcgc tcataaagcg cacaactttg cggtcaatga ggccagtggt
    53161 atccagtttt atgttgcccc agacgtggct acgcatgccg gaatccttca gctcctgaga
    53221 gaaagtgatc cctgaacgtc cttcacgcag agatgccagg acttcctgct ggttattacc
    53281 gatgctggaa acaatgccca ggccagtaat cactgcacgt ttcattcaat acctctgtaa
    53341 gtcgcacata gagtaagttt cgaatgcaca atagcgtaca cttgtacgcc gaacaagtcc
    53401 gatcagccat ttaatagaga tatttgcgca gccttacaca catcgctaag atcgagccac
    53461 cgcctgtaag acgagtaact tacgtgaaac actactccat acaacctgcc aacctcgaat
    53521 ttaatgctga gggtacacct gtttcccgag attttgacga tgtctatttt tccaacgata
    53581 acgggctgga agagacgcgt tatgtttttc tgggaggtaa ccaattagag gtacgctttc
    53641 ctgagcatcc acatcctctg tttgtgatag cagagagcgg cttcggcacc ggattaaact
    53701 tcctgacgct atggaaggca tttgatcagt ttcgcgaagc gcatccgcaa gcgcaattac
    53761 aacgcttaca tttcattagt tttgagaaat ttcccctcac ccgtgcggat ttagccttag
    53821 cgcatcaaca ctggccggaa ctggctccgt gggcagaaca acttcaggcg cagtggccaa
    53881 tgcccttgcc cggttgccat cgtttattgc tcgatgaagg ccgcgtgacg ctggatttat
    53941 ggtttggcga tattaacgaa ctgaccagcc aactggacga ttcgctaaat caaaaagtag
    54001 atgcctggtt tctggacggc tttgcgccag ctaaaaaccc ggatatgtgg acgcaaaatc
    54061 tgtttaacgc catggcaagg ttggcgcgtc caggcggcac gctggcgaca tttacgtctg
    54121 ccggttttgt ccgccgcggt ttgcaggaag ccggattcac gatgcaaaaa cgtaagggct
    54181 ttgggcgcaa acgggaaatg ctttgtgggg tgatggaaca gacattacca ctcccctgct
    54241 ccacgccgtg gtttaatcgt acgggcagca gcaaacggga agtagcgatt atcggcggtg
    54301 gtattgccag cgcgttgttg tcgctggcgt tattacggcg cggctggcag gtagcgcttt
    54361 attgcgcgga tgaagccccc gcgctgggtg cttccggcaa tcgccagggg gcgctgtatc
    54421 cgttattaag caaacacgat gaggcgctaa accgcttttt ctctaatgcg tttacttttg
    54481 ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca tgactggtgc ggcgtcacgc
    54541 agttaggctg ggatgagaaa agccagcata aaatcgcaca gatgttgtca atggatttac
    54601 ccgcagaact ggctgtagcc gttgaggcaa atgcggttga acaaattacg ggcgttgcga
    54661 caaattgcag cggcattact tatccgcaag gtggttggct gtgcccagca gaactgaccc
    54721 gtaatgtgct ggaactggcg caacagcagg gtttgcagat ttattatcaa tatcagttac
    54781 agaatttatc ccgtaaggat gactgttggt tgttgaattt tgcaggagat cagcaagcaa
    54841 cacacagcgt agtggtactg gcgaacgggc atcaaatcag ccgtttcagc caaacgtcga
    54901 ctctcccggt gtattcggtt gccgggcagg tcagccatat tccgacaacg ccggaactgg
    54961 cagagctgaa gcaggtgctg tgctatgacg gttatctcac gccacaaaat ccggcgaatc
    55021 aacatcattg tattggtgcc agttatcatc gcggcagcga agatacggcg tacagtgagg
    55081 acgatcagca gcagaatcgc cagcggttga ttgattgttt cccgcaggca cagtgggcaa
    55141 aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt gcgttgtgcc acccgcgatc
    55201 atctgccaat ggtaggcaat gttcccgatt atgaggcaac actcgtggaa tatgcgtcgt
    55261 tggcggagca aaaagatgag gcggtaagcg cgccggtttt tgacgatctc tttatgtttg
    55321 cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg tgccgagatt ctggcggcgc
    55381 agatgagcga cgaaccgatt ccgatggatg ccagtacgct ggcggcgtta aacccgaatc
    55441 ggttatgggt gcggaaattg ttgaagggta aagcggttaa ggcggggtaa tctgctctcg
    55501 cagtgtttgc cggatgcggc gtgaacgcct tatccaacct acgtgtgacg tgtaggcctg
    55561 ataagacgcg ataagcgtcg catcaggcat ggtgctccag acgccgcaac ttacagctgt
    55621 gacgcctgct gaaacaggtt ttcccacata tcggtcacca gcgcctggtc acgcggcgac
    55681 aattcaccgg caccaatggc tttttccaga ctctggctaa cggtcgtatg caccgcctga
    55741 gcggagtggt catcaccact ttccagttct gcgatggcta atgtcaggtg gccacgcaaa
    55801 tacccactgg caaacaactc atcatcactg gcatggtcaa ccatgccgtc gattaatgcc
    55861 agaatgcgtg attcaaactc cgcgatcatc ttctttcctc attgtaaaac gtggcgctga
    55921 cttcagttga gcgcttccgg ccacgggaac tgttccgccg taagttccgg cgtgtgataa
    55981 taattttgta gtgctttaat gaagcgtgcc gggcgttccg gaatgccatt tgtcagataa
    56041 tccatcacct gcgcatgtac ccgccgttga aacaccacac gatccggttc gaaatcccct
    56101 tccagattgt cgcaactgac gttaaacgga tatccagccg ccacgcagaa taaccagtcg
    56161 aacgcctgcg gcttcacttc aacatcttca aactggcttt gcgtctgggc atcgcgtccg
    56221 tccgggcaat accagtagcc gaaatcaacc agctcacggc gcgcttttcc ggcaatacac
    56281 cagtgcgaaa tctcatgaat tgcgctggca taaaagccat gagcaaagac gattcggtta
    56341 tacggtactt ccgcatcagc aggaagatag atcggttcgt cgtcgccttt aatcagacgg
    56401 gtattaaatt catcggcaaa gcagctatta aaaatttcaa tcaactgctc gtagtggtgt
    56461 gtactgttca ttagttcatc cccaaccagt ggaggatctc ctgtccgtgg ctatcgtaaa
    56521 gtaatttggc actcatcacc gccgagacga taacaatcat cgggcggatc agcttttgtc
    56581 ctttgctcaa caccaaccgt gaacccatgc gcgcgccgag gaactgcccc accagcatga
    56641 caaagcccgt cgcccaaatc actttgccgc cgagaataaa cagcagcaga ccgccaatgt
    56701 ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt ggcgaggttg aatccgcaca
    56761 gcataacgaa ggccagcgcg taaaacgatc cggcagccgg gccaaagaat ccgtcataaa
    56821 aaccgacgca gccaccggca attaacgcga agggtaaacc gtacatccgg cgctggcgat
    56881 cttcttcacc cagctttggc atcagcaaga aatagaggcc gatacaaatc accagaatgg
    56941 gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac caacagtgcg ccgctcattg
    57001 agccgacaaa ggtcatggca atattgagtt tctgatcgct taaactaacc actttgcggc
    57061 gaataaagta gatagtggcg gaaatagagc cgccgcaggc ttgcagttta ttggttgcca
    57121 gcgcgttagc gggagacatt cccgcagcca tcaatgccgg aatggtgagt aacccaccgc
    57181 caccggcaat cgagtcgata aatcccgcca gcatggcgac aaaaaagaga actcccagca
    57241 acagcggaga aaccataaac aggctattaa acgtttccat tagatcacgt gctcatccag
    57301 tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc ttctcaggct ttgttgttcc
    57361 cggttttgga ggttcaaacc agctttgcag ttctgccccg caaccatcgc ctgatggcgg
    57421 taaaggttga tcttcacact ccagactatc ggcagggcaa cgtaatcgta catgcatatg
    57481 cgcgcgatgc tggaaccagg gtcgcacttt gcgcaaccag tcgcgatcgg tgcccgcatc
    57541 aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg cgcgtgacgt ctttgtcctg
    57601 ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac agcgtggaga caacgtgttt
    57661 accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag agctgcgcgg aggtccagcg
    57721 agttttcggc agttgcagaa agatatcgac atccagtccg gtctggtggc tggcatgacc
    57781 gccgttgaaa cgcccaccag cgggcatccc catatcgcca atcagcaccg tacccatgcc
    57841 cagattgctc acctggctac tcagacgctg gataaacatc accagatccg ggtgaccgaa
    57901 atagcgacgc tgatcggtac gcatgacctg ataatgttcg gactgtatcg gcaacgtgtc
    57961 agcgccgaca atacagccat tagaaaaact gcctatcgat tgtgcgctac ccggcacagg
    58021 ttgggttatt ttttgccacg gcgtcgctgc caggctagcg ctactggcaa gcagagccag
    58081 cagcgcaatc gcggttttgt tcatttttta ccagcgtgga atatcagtct tcacatcggc
    58141 attttgcgcc cgctgccgta acaggtgatc cattaaaacg atcgccagca tcgcttcagc
    58201 gatcggcact gcacggatcc cgacacaggg atcgtgacgg cctttggtga tcatctcaac
    58261 ttcttcgcca aagcggttaa tagtgcgccc cggcacggta atgctggagg tcggtttcag
    58321 cgccatatga gcaatgattt gctgcccgct gctgataccg ccgaaaatgc cgcccgcatg
    58381 gttgctctgg aaaccgtctt tggtgatttc gtcgcggttc tggctgccgc gcagcgccac
    58441 tacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg ttgatgctca tcagcgcatg
    58501 ggcgatgtcg gcatccaggc ggtcaaagac cggctcgcca agtccggcgg ggacaccact
    58561 ggcaacaacg gtgactttcg cgccgatgga gtcgccctct tttttcagcg cgcgcatcag
    58621 ttcatctaac gcgtcgattt tgtcggggtc cgggcagaag aacggatttt gttcaaccag
    58681 cgaccagtct ttgatttcca gcggaatgtc gcccatctgg gtcaggcagc cgcggatttc
    58741 aataccaaat ttctcggcga gatatttttt ggcaatcgcc cctgccgcca cgcgcatggc
    58801 ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg cgcagaccgt atttttgttc
    58861 gtaggtgtaa tcagcatggc ctggacggaa gacgtcttta attgcgctgt aatcctgaga
    58921 acgctggtcg gtattttcga tcaacaagcc aatgctggtg ccagtagtaa cgccttcaaa
    58981 aacaccggag agtattttga cctgatccgg ctcgcggcgc tgggtggtat agcgcgatgt
    59041 cccagggcga cgacgatcaa ggtcatgttg caggtccgct tccgtcagcg gaatgcctgg
    59101 cggaacacca tcgacgatgc agccgagcgc cagcccgtgc gattcgccga aggtggttac
    59161 gcgaaagagt tgtccaattg tgtttccagc catcacggct ccgttattgt tgtgtttgcg
    59221 tgtttactta atctttataa atcgcgaaat gttctcgtgc ggcaataagc tgctctttag
    59281 tgagcataaa cacaccatcg ccgccgttat caaactccag ccaggtgaac ggaacatcgg
    59341 gatattgttc cataagatgt accatgctgt tgcccacttc acaaatcaac acgccatcat
    59401 cagcaaggta atctgccgcg ttaccgagaa tgcgacgcgt cagtttcagg ccgtcggtgc
    59461 cagatgccag gcccagttcc ggctcgtgac ggtattcgtt tggcaggtcg gacatatctt
    59521 ccgcgtcgac atacggcggg ttagtgacaa tcagatcgta ttgcactttc ggcaaatcac
    59581 ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag accgtgttct tcgatgttct
    59641 gttcggcaac cgccagcgcg tcgggagaga tatctaccgc gtcgacttcc gcttccggga
    59701 aggcataagc acaggcaatg gcgatgcagc cgctaccggt acacatatct aaaatatgct
    59761 gcggttgctt gctgatgagt ccggcaaatt tattgttgat cagttcacca atcggcgagc
    59821 gcggcaccag cacgcgttca tcgacgtaaa attcatgacc gcagaaccag gctttgttgg
    59881 tcaggtaagc caccggaatg cgctcgttga cgcggcggat cacgcgttca acaatacggt
    59941 gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc cgggatatcc agcggcaggt
    60001 agagcgatgg taacaccagc tgtacggctt catcccacgg gttatcggtg ccgtgaccgt
    60061 accagatatt tgccgcgctg aagcggctta ccgaccagcg caacatgtcc tgaatggttt
    60121 gcagctcatt tactgcttca tcaacgaaaa ttttatccac gtattcctcc agggcatgat
    60181 cgcaataatt tcggcggcta gtttgccatg aagatgacga taaatcagca ttcacgcgcg
    60241 gtgagtgagg aaaaatacgt ttaaaacgat cgattgtgct acgagtcggg taaactgtag
    60301 gaaaattaga aatagagaca gataaatgaa aaagaaaaca acacttagcg aggaggacca
    60361 ggctctgttt cgccagttga tggcggggac tcgcaagatt aagcaggaca cgattgtcca
    60421 ccgaccgcag cgtaaaaaaa tcagcgaagt gccggtgaaa cgcttgatcc aggagcaggc
    60481 tgatgccagc cattatttct ccgatgagtt tcagccgtta ttaaataccg aaggtccggt
    60541 gaaatatgtt cgcccggatg tcagccattt tgaggcgaag aaactgcgcc gcggcgatta
    60601 ttcaccggaa ttgtttttgg atttacacgg tctgacgcag ctgcaggcca agcaggaact
    60661 gggcgcgttg attgccgcct gtcgccgcga acatgtgttt tgtgcctgcg tgatgcatgg
    60721 gcacgggaag catattttga agcaacaaac accgctgtgg ctggcacaac atccgcatgt
    60781 gatggcattt caccaggcac cgaaagagta cggcggtgat gcggctttgt tggtgttgat
    60841 tgaagtggaa gaatggttgc cgccggagtt gccctgaatg ctggcagcaa atgccggagc
    60901 agcgtaaacg ccttatccgg cctacaaata tgtgataatt caataaattg caattcactt
    60961 cgtaggcctg ataagacgca tcagagcaat actacgaact tcagttcatt caatacatac
    61021 tgaaaacgac acgtcgaatc aatcggttgc ccactactgg tattcacatt caccacaata
    61081 tcgtgtagta ctttatccgt taccggatga cgagttggcg taaccgttac cgttgattta
    61141 tcacaaccgt aagaggtaaa ggttaacaat tctgataccg cgcggcagta tgctcctctg
    61201 ccatcattat cagaaaaatt actgccatca cagagtctgc cgccttccat caggtaggtc
    61261 gaaagcgtgg tggtcactgt tgaccccgtg gactgaattc gtaacgcctt attatggctg
    61321 acataatttt cactccagga gatatcgtat gaagcgaccg gtatcgttgg gcatattgtg
    61381 ttatgcactg taggggccgt atagcttcca aatccgggta aatctgtata ggaataaaag
    61441 gatgcgatcc aagggcaacc ataatcatca gatgcaacgg taattttaaa cgtcatatat
    61501 gcacaacgaa aaattttcgt atctatgcca gggcaatttg agttattaat tgtatcaaag
    61561 tcagtcagta ccaaagttgg agctgaaccc gacccagacc aggaaataac ccgcaacgta
    61621 tcgcctttat acgcaaaaga atcatatttc ccatatgcaa cctcctttgg cgttatcacc
    61681 gtaacattga aaacactgct cgctacagca ccggttacct tgtccatggc ataataaact
    61741 ctcacactgt caaaactcgc actcataagt ttatctccag cataactctt agatgcgccg
    61801 acaacactca gaacgagcag cattaggctc gcgaaaatta tggtccattt tttcatttca
    61861 gaaatactca aaggttgccg tcactgtggc actaaagtct ccggacgtta catctcgccc
    61921 gctctttgcc tgtaaccagg tattaaaatt taggctgtta tttccctgat tcagcgggag
    61981 cgtgacgcct gttgtattgt tgattggcac cgatgtgcct gccgcagttt caataccaat
    62041 cccagcgccg gatgctgatg acgaggagtc aaaggccaga aagccgggta gagcactatc
    62101 ttctgttcct gtcaacgtta ctttgacatt aaacaatgta gaactatgac aatctttcaa
    62161 ttgaaacacc aatggcactc gctctgactg cccaaggttc attaaatcac tggcggcaat
    62221 ggcaggaaaa tgtacttctg ccaactggct accattaatc actggcgtac acgatttact
    62281 gattaaattg ccggtaaagt gcaaattatc tcctgctatt gtggttccac tgcccagcaa
    62341 catcaggcta ctaataaaaa acaacgtacg ctttttcttc attggtaatc tacctgtaac
    62401 gtggcccagg cctcaaagtc cgcctctggc agaatgacac cactcttttt tactggcaca
    62461 gccgttaaca ccggtaaatc ggtttcattc accacaagtg gcgtattgat cgtaaaactc
    62521 tgccctgcct gtttgagctg aatgcctaaa ccagtgatat tgctgcttac tgcagcatta
    62581 tcaaaatcgc ttggtgtgcc actccagctc aaggtcattt ccattaatgg gtcgcgaacc
    62641 gccgtatcgc aggtaatgga atacggcacg gtttgctcat aattgctgcc attaatgtca
    62701 tcaataagca catcctgaaa agttatttca atggtttgag cgttgctaat cgtgcagtta
    62761 ggcggctgca ctaaataacc gtgaaaagag acgtttgaga gtgccatcga gcaaaagccc
    62821 cacagcaaaa taagcgagat ccgtttcatt ggtaatccac cacaattgtg gcactggcgt
    62881 taaattcagc tgccgctaac gttgtaccac tctgtttcac cggcacagct tccagaacgg
    62941 gaactcccga ggaaaaatta aatggcaacc atgagccaga ggttaagtcg agtattgtgt
    63001 gatcactact tttctgaacc cgaataccaa acccaggaat acccgtgctg ataactgttt
    63061 caccgttaat cacgaccgtt gtcgcctgca tctgtagacg caggtcatcc attgccagat
    63121 tgttacaaac aagcgagtag tcaatgggac gtttataatc aaccctattg atcttattaa
    63181 tgaatacatt accaaactca actgccgcac catttaccgt acaaggagga ggggcatcga
    63241 caagcacccg taaattcata cttactgact tactcgctgc aactgcaaag ccgctaccaa
    63301 cacaacatat cagtaaccca gttaccgccc agacagaaca agatcctttc atactggctc
    63361 tcccggcctc agcctttgcg gctctgctct tcatcgacct agcaactgtt accgttgcaa
    63421 gtgaaaaaga gcggcatccg agcgccatag tcgttgacat aggtcagtac cggcacgcta
    63481 cccatcttca cattcagcgg tgcagaggtt ttcgccgaca tcactattag cgagaaaccg
    63541 gctgccggat tgccattttt ctgtgtgctg gcattactga aaatgacgta atacgacgtc
    63601 gggttattga cggtaaattc ctgaccgttg cgggttaaag taatcttata ttgctagggg
    63661 tttttcatgc tgacgttctc cagcgccttc ggtcgccaga acaacttaat acgcgtttgt
    63721 agtgctattt gcagcgtgtt ggctttgttt gattttggcg ggatctcgcg cacgttgaag
    63781 tagaacaggc tttcacgatc ggcaggcaat ttgttgatat ccggcattcc ctgcactttt
    63841 acctgcccgt tcatcattga atcgatgcgt tgcaccggtg gcagaacagt taacggtgaa
    63901 ctgatcttgt tacccttttc atcttcgatc cagctttggg ccagataagg cagtttcgga
    63961 tcgttattgc gcaacgtcac gctgattgat ttgtcgcttt cattaaatat caaacgggta
    64021 cgatctggcg ttacagaagc ctgggcagac aaactggcag cgctaagcag taacgctagc
    64081 gtcgtagcac ccaatttggc agagcaaagt aaatcagaca tggatcattc cttaatcacg
    64141 ttattggtta gctgaaacag aaacaggggg attggtgggt gtcacctgtt gcgtctgctc
    64201 ctgaatgaca ggctttatgt catcaggaac aaccggcgcg acgttgcctt tatgctggca
    64261 tggcagtaac aagccgttaa acagatcggc aggcagcggg tccggcaggt tgatatcgca
    64321 atgcgcaaca ccactccaga acacactcat gtgttcgcca ggtttcaccc cagccagata
    64381 aacattgccg tcatcatcga caaggcccac tgtctgctcg ttatcatttt ttacttctgc
    64441 gccaaacgga ggatggctgc cgtcttgtaa acgcagcacc gccatcgctt tttgaccact
    64501 gatgacggca aatttgcggt agccaatcgc cccttcagtt agcgtggctt gtaccaccga
    64561 ctgggttgct tcggcgtttt ccggcagttt gttgaggtcg atatacgcct gattgcggta
    64621 atagttattg acgtcagaaa cgacggcttt accaaacata ttggtataaa cagcagcacc
    64681 gttaccttct accggaacat cggcaacgcc atcggcatca atcaacaagc gtgtcccgcc
    64741 catattctgg gtacggtgaa gtgcgccacc gtgggcagta agcgttgcgc cgccctgtaa
    64801 cgacaagccc gcagaggtgt attgcccttc atggtagttc gcactgaggt caacctgcgc
    64861 cagcgaacca tcatggctgt aatagccgtc aacgctggtg tgtttgtcac tggtgccgac
    64921 gttcaactga tagtgagtcg cgtcatcgac acggctgaaa taaccgacct gactgctgtc
    64981 cgtcccactg ccatagtacc gttatagcta acggtactgt tgtcgcccca cggcatactg
    65041 agcgaaatgt acatgccttt gtcggcctgg ttgtcatact cgtaacggta gccagtcatc
    65101 gagatgctga cattacgaat actgcccata ttgaaatagt gcgagagcat gatgttgtag
    65161 tttgtctgct cctcgcgatc ccagtaggta tggcgggtat agttgagata aaccgaaaca
    65221 cccgcatcgc ggaagttctg gttataagtg gcggtgtaca tctctttgtc gttgcccgtg
    65281 cgtaccattc cgctgtcact ggcatccaga tactcgctca tggtcataaa gttctcttcc
    65341 gagaagcgat atccagcaaa agtaacgcgg ctgttgagct ggtcaaagtc tttggaataa
    65401 ctcacacgga aggagttacc gtccagcgaa cctttgccat aagcggtgtc tttatccagt
    65461 ttggtgtgcg agtgagtaac atcaaacgcg accgcgccga atgtagacaa atcgcgaccg
    65521 acaccaagcg ccgcagactg atagttttca tctcccagtg cgccgccata taacgaccag
    65581 ccgttagcga tcccccagga agcttcagca ccagaaaaaa attcaccctc gacatggtgt
    65641 ccccactctt gcggacggcc catcatgatc ttatagcgaa cctgacctgg acgagtgagg
    65701 tatggcatcg aggcggtgct gatgtcatat tcctgcacct ggccgttctg ttcttcaatg
    65761 cgaatatgca acgtaccgga gacagaatca ccaagatcct gaatacgaaa cggtccagcc
    65821 ggaacctgcg tttcgtaaat cacacgcccc atctggctga cggtcacttt tgctgtggtg
    65881 tgtgccacgc cggaaatgtc tggcgcgtag ccgcgcagat tgggaggcaa catttgatcg
    65941 tcagtactga cactgccacc aacatagtta aaaccatcaa aaatatcgga tctgaggtaa
    66001 tcctcgccca gcgccagttt ggcttttaat gatggtaacg cccgcctggc atagtagcga
    66061 ctccactccc attttttttg agtttcatcg ccgctgaatt catcgtcatc attactgcga
    66121 gtatgttgat agttagtctg ccagtcagca cgcatacgcc acggccctag gttaacccca
    66181 accgtcccgt tgccgctgat ctcgttacta tcatcaccgc cattttcttc gtgccgggtt
    66241 tgtgcgttga tgctgtagtc cgcgacgatc ccggagatgc cgtcatccca acgtgaaggc
    66301 ggatcccagt cgggataagt atattcgagg taagcctgtg gcagtgaaat gactaatgcg
    66361 gactggctta aatcagcctt aatttccatg ccttccagtt gaccggattt caggcattta
    66421 gcatcgtggc tccattgcag atttttcgcc acgtcttctt ttaaaccaaa ctgcgctacc
    66481 agttccggtg tcagacaagc atagcttttg ctcgcgtcat cttcaccagc ataccagtaa
    66541 atatcgtact cttccgccaa tggctgttta tttagttgaa cctgtaaatt gtattttccg
    66601 ggctcaacat atccctagct ggaaaaacgc ttcagatcaa tttttgtgtc gccttttaat
    66661 tccagaaaac gggaatcaaa ctgaatgtcg tcttcagccc agacactgct ataactccct
    66721 gacattgcca gcgcaatgca ccacggaaga atccgtaatc gaaaaagaga atggtcaggc
    66781 atacacgttt ctatccgttt ttattcttgt tcggtcaaaa tacatttaaa tcccccatag
    66841 cagtgtcgct atgggggtat gccttacagg taagtaatct ggaaggtggt atttgcttca
    66901 aaattaccca gatctggcgc atcagctgcg ccgaccagcc aggcgttaaa gttcagtgtc
    66961 tgcttcgctt tgcctttgtt ggtagaagtg tctttcacta ttttctggtc aatacccatg
    67021 ccgcttttgt aagaggtacc cagagagtca ccgatcgcca ggctaacatt gttaaatgcc
    67081 gcaccggtat cggtattgaa aatggtgtaa taattgccgc tatttgcaga agaaacggta
    67141 ccagtaaagg tagtggtcat tgtttcctga gtatcaaata cgcaatcttg cagacgaatc
    67201 tggaaacttt tcggcgtggt ggtgccgtta tttttcagtt tttcggcacc gatatcaccc
    67261 atatcaactt ccagtttgtg atcgtctgga acgatagaac acactgagtc ttcaatggtg
    67321 ccgtaaaaac gtgcagtacc gttgttaccc gcagcgattg ttgccgtaga ggtgaacacg
    67381 cccatcacca ttgccgcagc aatagctgtt ttaacaaact tactcattat ttgtccttaa
    67441 actataaata attaaaaata taaacattgc aatacattga ttcagtcaat agccaatgtt
    67501 tatacccagc cattgaatac ggcatgcgac ccaaaaagaa caggtcgtag aaaattgtat
    67561 tcattggtgt ggggccgcat agtgatgttt ccacacccct gatccaattt aattttacta
    67621 aagagtaaat gtagatttaa ttaatatatt gacagaagaa aggttatctt aaggacatcc
    67681 ttaaaaaata cggaataagt atttagaaaa aatagaaagt atatcaataa tgaatatcaa
    67741 cagcttaaaa aacaaacaat gaacatataa tgcgattggc atttatccac ttttgacaat
    67801 ataatatata aacactatag ccagaattta atcaagtttc aattacatcc tgcctgatta
    67861 ttcaactcta ttttctctat tatctgtatt aataaatgac aatgagtgtc aaacagatat
    67921 tattcatttc aacatgtaac tttgggaaaa atcaagatac gcgcagaaga ataatttccg
    67981 aatgaatgta gaaagtgttt tatttataga taaggagagt cttcgatagt agtgaggttg
    68041 acagatatcc gctgcctaat gagcaattat gcccaatgaa tctacctcat tgggcataat
    68101 ttggaacaca tcagatagct tttgccatct tcagattaca cgggctcatc tgccagttga
    68161 atgtaccgtt tccgctttca tcaagcgtta cgctggcaat ggccgaggtg gtaaacatag
    68221 gcggcgtttc gcccggacat aactcggcaa ccagataccc gactaacggt aagtgagaga
    68281 tcaccagtac cgaagcaaca ccttcattgg tcaacgcctg caaataggca ctgaccagac
    68341 caacatcgcc gcagggcgtt aactccggca gaacttctgc actggaaggc aggttcagac
    68401 aatcccctac ttcttccagt gtttgctcgg ctcgcaggaa cgggctcacc agaacgcgtt
    68461 cgatttccac tttttgacct ttcagccagt tcgccatcag gcgagattcg tcacaaccat
    68521 tagtggtcag aggacgaacc gaatcactgg cggaatcgag ggctgcgtcg ccgtgacgca
    68581 tgataaaaac ttgcatattg caccgctttt gttaaccaga ttcgcccgtt ttctttcatc
    68641 tgaaaccaga gaaagaaaac ggtggccggc attgtgcctt atccattcac cgaatgaaac
    68701 gctgtttttt acctcaatgg cgtaagtata gtcaatatgc gtttacattt tgaccaacac
    68761 tccgccattc agcgcggatt catatagctt tgaccttctt attgcaggtc agttgcagtt
    68821 gttttccaaa aactttcccc acgcgcgccc atctcgacca aacgctcgca aggggtaaaa
    68881 cgggaaccat actgcgtggc cagtcgttgc attattgcaa ccacttcgcc cgcgccgaga
    68941 gaatcgatat agcggaacgg tccaccgaga aatggcggaa aaccaatgcc aaataccgca
    69001 ccaatatccc cgtcacgcac gctacggata acctgctcat caacacaacg tactgcttca
    69061 ttcagcatca acatcacaca ccgttcagca acctgcggtg cggagagtca cccctgccct
    69121 tgtgcgccaa tgagcgggta aatggcagga tcgacctgtt ttttgctttt acgccctttc
    69181 tgaccataaa gatagaaacc ccgaccattt tttctgcctt tgcgatcgtc gttcaaaatt
    69241 gaagaaacaa catttgcagg cgcgctaaaa cgttctccat aagcggcttc cagtacagga
    69301 ataattttag tcccggtgtc gattcctacc tcatccaaaa gttggattgg gcctaccgga
    69361 aaaccaaatt tcactagcgc ggcatcaatg tgctctaccc gttcaccttc ggtcagcatg
    69421 cggatagctt cattaatgta aggcgctaag atgcgattga cgtaaaaccc ggctttgtca
    69481 cgcacgacaa tgggcgtttt gccctgtttt ttcgccagtt ttaccgtagt ggcgatggtt
    69541 tgcgccgatg tacccgcatg aggaataatc tctaccagcg gcattttttc caccggactg
    69601 aagaaatgca ggccaataac ttgctcaggt cgcgcggcgt gagcggcgat atcaccaatc
    69661 ggtaaagatg acgtgttcga agcaaaaatg gtatgagcgg cgcaattttg ctccacttcc
    69721 gccaccatct gttgttttaa ttcgagattt tcaaacaccg cttcaataat cagatcgcga
    69781 tgggcaaagc cgcgatagtc cgtcgttccg gagattaatg ccagctgttt gtcacgttcg
    69841 ctggctttga gatgacgacg gcgaactttg ccctccagct gatcccaact gtacttcagc
    69901 gcatgattta tgccctgcgg gttgatatct ttaattctga ccggaagccc cgctttacaa
    69961 gcagtgacat aagcaatacc gccgcccatc aagccaccac ctaaaattcc cacgctgttt
    70021 aatgccgcag gcggtgcatc actaccggga tctttcttca cgtccgtact ggcaaaaaag
    70081 atactacgca aagcttgcga ttgcggcgtc atcgccagtt cgccaaaggc gcgagcttca
    70141 gcgtcatagc cgctgctggt gccctgcgct aatcccgttt caacaacctc caggatgcgt
    70201 tctgtcgccg gataattgcc ttgagttttg tgttctgttt tcttgccgac cattttgaac
    70261 agcagcgcac gacctaacgg ccccgccaga atacgctcgc gtacaggtag agggcgggaa
    70321 gatgggcgat ccttcttcgc cagctcaaca gcggcttcca gcaaaatgga gtgcggaaca
    70381 acgtcatcca ccagccccag ctttaatgcc tgtttcgccc gaagttgttt tccggtgaga
    70441 atcatctcta atgctgtact gacgcctatc agacgcggta aacgctgggt gccgcctgaa
    70501 ccgggtaaca atccaagttg tacttcagac aaaccgagca ccgttttagg atcgtcagta
    70561 caaacgcgac cgtggcacgc cagcgccagc tccagcccac cgcccaggca agcgccatga
    70621 atagccgcga taaccggaat gggcaaagca tgaatctccg ccatcaactg ttggccctgc
    70681 cgcgccagtg cttccgcttc ttgcgccgtt ttgcagttgc caatcatgtt gatgtctgct
    70741 ccggcgataa aattgtccgg tttcgcggag ataaacacca cgccgcgcaa ctctttgttt
    70801 tcacggagtt gcttaataat ggcgcgcacc tgcgaggcaa attccgcctt cagggtattc
    70861 attttctcat ccggtacgtc gatggtgatg atagcaatgt tgtccagacg aacattaaga
    70921 gtaaacgctg atgccatttc cattattccg cctccagaac cattgccgcg ccaagcccac
    70981 cggcagcaca ggcggtaact aaaccaaatc caccgccgcg acggcgaagt tcatgcaatg
    71041 tctgggtaat catccgcgcg ccggtcgccg cgaagggatg tccgtaagca atcgaaccgc
    71101 caagcacgtt aaatttgcta tcgtccactt cgccagtggc atgtgcacgc cccagtactt
    71161 cacgagcaaa acgttcacta cccagcaact gaatattcgc cagtgtctga gctgcaaagg
    71221 cttcgtgcat atcgatcaat gtcagttcgc tcatcgtcaa accggcacgt tccagcgcca
    71281 gcggtgttga ccaggctgga ccgagcaaca tgtcctgcca gacatcaatc gcagtaaatg
    71341 cgtagctgcg cagatacccc agcggcacca gccctaattc tttcgcccgg gattcagtca
    71401 tcaggatcac cgctgccgcg ccatcggtca gcggcgtact gtttgccgcc gttaccgttc
    71461 cgtgtttgcg atcaaacgcc gggcgcagct ttgcgtaatc ggcaagcgag gaattaccgc
    71521 gaatattgtt gtcttcgaca agcggttgtt tataaggagg gataaaggca gtcatcacct
    71581 cttctttgag ttttccgtct gaccatgcct gagcggcacg ctgatgcgaa cggtgcgcta
    71641 atgcatcttg ctgttctcgg gtgatgccgt aggttttcgc catttgttcg gcggtgtcgc
    71701 ccatccgcaa gccagtagaa tattctgcta ccgcaggcgg tacgggcatt aagtcgcgca
    71761 aacgcaggcg agagaagagt ttcaggcgct ggctcatggt tcgagctttg ttgacatcaa
    71821 ccagcacgtg cgccagtttt ttactgacgc caattggcaa tacggaagag gaatctgccc
    71881 caccggcaat ccccgctcgg atagttcccg ccatcaggct ttctgcgacg tttgcaaccg
    71941 cctggaaact ggtagcgcaa gcgcggctga cgctgtaagc atcggtatgc acattcattc
    72001 cggtaccgag aacaatttca cgcgcaatgt tgggggcttc aggcatttgt acgacctgac
    72061 caaagaccag ctgttcaatc acttcagcgg ggatctcgct gcgtgccagc agttcgccta
    72121 ccaccatctt ccctaaatca accgtgggaa tgccatgaaa agccgtcgcc tggcgggcaa
    72181 aaggcgtacg taaaccgcta acaatggcga tacgatcgcc ctggcgggta accagtggta
    72241 aaacctgacc cataacactc ccctgtaaaa aaataaataa agtggtctga cctgatcata
    72301 gtcttaacca tttttttaca tttagccaag cggagaaaaa ggaaagtggg agctatgaca
    72361 cagtgaagac agaagaaaga aaagaaaacg cccctgccag caactgacag gggcgtacgt
    72421 acccgagagg aattaacgca gacccagctg gaagatcagc atttcagctt cgcaggcaaa
    72481 agtgaaatcg atatccaggc gcacaccgtc agactcttca gtgaaagtcg gggagatttt
    72541 gcacggttcg gattccacgc tacgggattt ttcagtcagc gccgccagcg tttgttctgc
    72601 ttctgcgcgg tttgcaaaca cgcggctgta agacgcggtg cagtcggagt tgtccataat
    72661 ggtgccaaca tccatacagc agcaaaccgg ggtttcatca gcactacatt tactcatcgt
    72721 tgatttcctc tgtatgtgca cccaaggtgc cagataaacg ttgtggatat tttacgcttc
    72781 cggaaagtgc tgctccagtt gttaattctg caaaatcgga taagtgaccg aaatcacact
    72841 taaaaatgat ctaaaacaaa attcacccga atccgcgagt gcgcaacctc caaattttgc
    72901 cagctggatc gcgtttctta gatcatattt gaaaaaagat ggaaacatac ttgcaacatt
    72961 ctagctggtc cgacctatac tctcgccact ggtctgattt ctaagatgta cctcagaccc
    73021 tacacttcgc gctcctgtta cagcacgtaa catagtttgt ataaaaataa atcattgagg
    73081 ttatggtcat gagccagaaa accctgttta caaagtctgc tctcgcagtc gcagtggcac
    73141 ttatctccac ccaggcctgg tcggcaggct ttcagttaaa cgaattttct tcctctggcc
    73201 tgggccgggc ttattcaggg gaaggcgcaa ttgccgatga tgcaggtaac gttagccgta
    73261 accccgcatt gattaccatg tttgaccgcc cgacattttc tgcgggtgcg gtttatattg
    73321 acccggatgt gaatatcagc ggaacgtctc cgtctggtcg tagcctgaaa gccgataaca
    73381 tcgcgcctac ggcatgggtt ccgaacatgc actttgttgc accgattaac gaccaatttg
    73441 gttggggcgc ttctattacc tctaactatg gtctggcaac agagtttaat gatacttatg
    73501 caggcggctc tgtcgggggt acaactgacc ttgaaaccat gaacctgaac ttaagcggtg
    73561 cgtatcgctt aaataatgca tggagctttg gtcttggttt caacgccatc tacgctcgcg
    73621 cgaaaattga acgtttcgca ggcgatctgg ggcagttggt tgctggtcag attatgcaat
    73681 ctcctgccgg gcaaactcct caagggcaag cattggcagc taccgccaac ggtatcgaca
    73741 gtaataccaa aatcgctcat ctgaacggta accagtgggg ctttggctgg aacgccggaa
    73801 tcctgtatga actggataaa aataaccgct atgcactgac ctaccgttct gaagtgaaaa
    73861 ttgacttcaa aggtaactac agcagcgatc ttaatcgtgc gtttaataac tacggtttgc
    73921 caattcctac cgcgacaggt ggcgcaacgc aatcgggtta tctgacgctg aacctgcctg
    73981 aaatgtggga agtgtcaggt tataaccgtg ttgatccaca gtgggcgatt cactatagcc
    74041 tggcttacac cagctggagt cagttccagc agctgaaagc gacctcaacc agtggcgaca
    74101 cgctgttcca gaaacatgaa ggctttaaag atgcttaccg catcgcgttg ggtaccactt
    74161 attactacga tgataactgg accttccgta ccggtatcgc ctttgatgac agcccagttc
    74221 cggctcagaa tcgttctatc tccattccgg accaggaccg tttctggctg agtgcaggta
    74281 cgacttacgc atttaataaa gatgcttcag tcgacgttgg tgtttcttat atgcacggtc
    74341 agagcgtgaa aattaacgaa ggcccatacc agttcgagtc tgaaggtaaa gcctggctgt
    74401 tcggtactaa ctttaactac gcgttctgat aacgcgttcg cctagataaa gtcacctgct
    74461 atgcaggtga ctttaactcc cccacttcac cgaaagtagt ttcctcattg cttgcctcct
    74521 gagttttgca aacagcctga tggcagcttg ccctttcaaa tcagtaagta gtatccacag
    74581 acaaacctga agggaaatgc atattttcaa gcgttctgct cgtccttctc aaagagttta
    74641 cttttctgca tttccaggat actcaacccc cctggctatt gtgcgcttat acactcaaat
    74701 taaagatagg ttctaaataa atgagcgttt tttgatagtc tatttcatta ggtaatatat
    74761 atttgtaaca aatcaatcaa catggaataa aatcatgcta ccatctattt caatcaacaa
    74821 taccagcgca gcttacccag aatccatcaa tgaaaataac aatgatgaaa ttaatggatt
    74881 agtacaagag ttcaaaaacc atttttaatg gtaaggaagg aataagcacc tgtattaaac
    74941 atctacttga gcttataaaa aacgccatac gagtaaacga cgatccttat agatctaata
    75001 ttaataattc ctcagttact tatattgata ttggctccaa tgatacagac catattacta
    75061 ttggtatcga caaccaagaa ccaatagaat tacctgcgaa ctataaagac aaagaactcg
    75121 tccgtactat cattaatgac aacatagttg agaagactca tgatatcaat aacaaggaaa
    75181 tgatcttcag cgcattaaaa gaaatatatg atggagatcc tggttttatc ttcgataaga
    75241 tatcacacaa actcagacat acggtaacgg aatttgatga gagcgggaaa agcgaaccaa
    75301 cggacttatt tacctggtac ggtaaagata aaaaaggcga ctctctcgct attgtaatta
    75361 aaaataaaaa cggaaatgat tacttatttc tcggttacta cgatcaggac gactaccaca
    75421 ttcaaagagg aattcgtatt aatggtgata gtctcaccca atattgtagt gaaaacgcca
    75481 ggagtgcttc agcgtggttt gaaagcagta aagctatcat ggcagaatca tttgcaactg
    75541 gttccgatca tcaggttgta aacgagctca acggggaaag actgagagaa cctaacgaag
    75601 tttttaaacg tttaggtcga gcaataagat ataattttca agtggacgat gcaaaattta
    75661 gacgcgataa tgtaaaagaa ataatttcta ctttattcgc taacaaagtt gacgttgacc
    75721 atcctgaaaa taaatacaaa gactttaaga atctcgaaga taaagttgaa aaaagacttc
    75781 aaaatcgcca gacgaaatat caaaatgaaa ttaatcaact atctgcacta ggtgttaatt
    75841 ttgatgatat ttaaaattat ttttccagtt ttttgatttt tcaacgcctg aatatcggaa
    75901 atagtcagtg acaaacatca gtatttttac atcattacgt ccgggtcatt gtcggtttat
    75961 ctccttttac ttgtggtaat tgagggataa caacaaaaaa ggtgagtttt gcgactcacc
    76021 ttttttattt gtttcttatt cagaatcgat atcttttaaa tcatcctgaa tcgcttgcgc
    76081 gttcggattt tcctgcggtt tgagttcgcc gccattagcg atgaaatcat gacgctggaa
    76141 gtacgcttcg cgcaccataa tataaggatc ggacgactga cgcagcagac catcggaatc
    76201 cagcagctga gcgcgggttt cgatcccttc aagcgtccat ttacccacag acatcggcca
    76261 ggtcagccag gaaagaaccg ggtaaagagc atccgccata tcaccaccgt catcacgcag
    76321 cgtgaagcta ccgtagaacg gtaactgaac gtaaggccca taacccacgc cataatgacc
    76381 aagcgtacta ccgaagcggt gaggttcagt ccgttgcagt ttcgggttcg ccatccctgc
    76441 aacatcaata aagccgccca tccccaaaat ggtgttcagg aaaaagcggg taaagtggac
    76501 catcccctga taagggtcgc cctgcaagaa gtagttaacc atcaccgcag gttcttcaag
    76561 gttgccagta aagttgctca aaccattacg cgccggttgc ggaacataat cacgccaggc
    76621 gacagcgacc ggtcgaacaa tatacgggtc taatacgttg aagttgaagt tgtacatggt
    76681 gcggttgaac ccttctaacg ggtcagaacg cccttgctga tctgtaccgg aactcgcaca
    76741 ccccaccaga agcgtagttc ccagagcaag cgccgacagg cgaagcttca taaatgtctc
    76801 cctgtttttt tatggcttat gcagtttgcc atccatgacg gaacgatacc gtatccgcct
    76861 gtttaggtgt gggcgattgt aacagcacgt caactgatgt ccagacgccc tgatttgctg
    76921 atttgatcat agcctggtaa tcgccgccct gtaggctact tgattctata gaaacagaaa
    76981 aaggcaaacg cagccttttc tacattttca gagtaactcc cgccagttgc gagcaaaaaa
    77041 gccgctacgc tttagctata cgtgctaatt caagagaaga gaccatggac aacgacaaaa
    77101 ttgatcaaca cagcgacgaa attgaagttg agagcgaaga aaaagagcgc ggcaaaaaaa
    77161 tagaaataga tgaagaccga ctcccctccc gagcgatggc aattcatgag catatccgcc
    77221 aggatggtga aaaagagcta gaacgcgacg caatggcgct actgtggtca gccattgcgg
    77281 cgggtctgtc gatgggcgct tcgttactgg caaaagggat atttcatgcc gaactggaag
    77341 gtgtgccggg cagcttcttg ctggagaatc tcggttatac ctttggtttt attatcgtca
    77401 taatggcccg ccagcaatta tttaccgaaa acaccgtgac tgcggtacta cccgtcatgc
    77461 aaaaaccgac aatgagcaac gttggcttac ttatgcggtt atggggagtc gtgctgctgg
    77521 gtaatattct cgggactggt attgctgcgt gggcatttga atatatgcct atcttcaatg
    77581 aagaaactcg cgatgcattt gtcaaaatcg gcatggatgt gatgaagaac acccccagcg
    77641 agatgtttgc caacgcgatc atttccggct ggctgatcgc cactatggta tggatgtttc
    77701 ctgcagcagg tgcggcaaag attgtggtga ttatattgat gacctggctt attgccctgg
    77761 gtgacaccac ccatatcgtg gtcggttctg ttgaaatcct ctatctggtg tttaacggta
    77821 cgctgcactg gagcgatttc atctggccct tcgcactacc tactttagcg gggaacatct
    77881 gcggcggcac ctttatcttc gcgttaatga gtcatgcaca gattcgtaac gacatgagca
    77941 acaagcgtaa agcagaagca cgccaaaaag cagaacgtgc ggaaaacatt aagaaaaatg
    78001 ataaaaaccc agcataaatg gcgagggttt aagcaatcga gcggcagcgt acttaccccg
    78061 cagtccatta gcgggtatac tcgccgcatt gtcctcttag ttaaatggat ataacgagcc
    78121 cctcctaagg gctaattgca ggttcgattc ctgcagggga caccatttat cagttcgctc
    78181 ccatccgtac cagtccgcaa aatcccctga atatcaagcc ttccgtatat tcacagttcg
    78241 tcatggttcg cgtcagatcg ttgacagccg cactccatga cgggtaaaaa gtggataaaa
    78301 taattttacc caccggattt ttacccatgc tcaccgttaa gcagattgaa gcagcaaagc
    78361 cgaaagaaaa accataccgc cttctcgatg gtaatggcct gtacctttat gtccctgtat
    78421 ccgggaaaaa ggtatggcag cttcgctaca agattgacgg taaggagaaa atcctgaccg
    78481 tcggaaaata tccgcttatg actttgcagg aggcaaggga taaagcatgg actgcgagga
    78541 aagacatctc ggctggcatc gatccggtaa aggcgaaaaa ggcttcgtct aacaacaatt
    78601 catttagtgc gatttacaag gaatggtacg aacacaagaa gcaagtctgg tcagtagggt
    78661 atgcgactga acttgcaaaa atgtttgatg acgacatttt acccatcatc ggcggccttg
    78721 agattcagga tattgagccg atgcaactgc tggaagtaat ccgcagattt gaagatcgcg
    78781 gtgc
//
LOCUS       DAECNO010000003        75876 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.003, whole genome shotgun sequence.
ACCESSION   DAECNO010000003 DAECNO010000000
VERSION     DAECNO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.003"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..209)
                     /locus_tag="KKO08_000183"
     CDS             complement(<1..209)
                     /locus_tag="KKO08_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316597.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDR"
     gene            complement(211..431)
                     /locus_tag="KKO08_000184"
                     /pseudo
     CDS             complement(211..431)
                     /locus_tag="KKO08_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(424..645)
                     /locus_tag="KKO08_000185"
     CDS             complement(424..645)
                     /locus_tag="KKO08_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316598.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(635..835)
                     /locus_tag="KKO08_000186"
     CDS             complement(635..835)
                     /locus_tag="KKO08_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBD6316599.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            965..1144
                     /locus_tag="KKO08_000187"
     CDS             965..1144
                     /locus_tag="KKO08_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316600.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1269..1598
                     /locus_tag="KKO08_000188"
     CDS             1269..1598
                     /locus_tag="KKO08_000188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316601.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1648..1836)
                     /locus_tag="KKO08_000189"
     CDS             complement(1648..1836)
                     /locus_tag="KKO08_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBD6316602.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2211..3439)
                     /locus_tag="KKO08_000190"
                     /pseudo
     CDS             complement(2211..3439)
                     /locus_tag="KKO08_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3688..4809
                     /gene="roxA"
                     /locus_tag="KKO08_000191"
                     /gene_synonym="ycfD"
     CDS             3688..4809
                     /gene="roxA"
                     /locus_tag="KKO08_000191"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBD6316603.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4858..6084)
                     /gene="pepT"
                     /locus_tag="KKO08_000192"
     CDS             complement(4858..6084)
                     /gene="pepT"
                     /locus_tag="KKO08_000192"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBD6316604.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6334..7470
                     /gene="potA"
                     /locus_tag="KKO08_000193"
     CDS             6334..7470
                     /gene="potA"
                     /locus_tag="KKO08_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBD6316605.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7454..8311
                     /gene="potB"
                     /locus_tag="KKO08_000194"
     CDS             7454..8311
                     /gene="potB"
                     /locus_tag="KKO08_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBD6316606.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8308..9102
                     /gene="potC"
                     /locus_tag="KKO08_000195"
     CDS             8308..9102
                     /gene="potC"
                     /locus_tag="KKO08_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBD6316607.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9099..10145
                     /gene="potD"
                     /locus_tag="KKO08_000196"
     CDS             9099..10145
                     /gene="potD"
                     /locus_tag="KKO08_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBD6316608.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10301..11122)
                     /gene="cobB"
                     /locus_tag="KKO08_000197"
     CDS             complement(10301..11122)
                     /gene="cobB"
                     /locus_tag="KKO08_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBD6316609.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11138..12049)
                     /gene="nagK"
                     /locus_tag="KKO08_000198"
     CDS             complement(11138..12049)
                     /gene="nagK"
                     /locus_tag="KKO08_000198"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBD6316610.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12078..13322)
                     /gene="lolE"
                     /locus_tag="KKO08_000199"
     CDS             complement(12078..13322)
                     /gene="lolE"
                     /locus_tag="KKO08_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBD6316611.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13322..14023)
                     /gene="lolD"
                     /locus_tag="KKO08_000200"
     CDS             complement(13322..14023)
                     /gene="lolD"
                     /locus_tag="KKO08_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBD6316612.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14016..15215)
                     /gene="lolC"
                     /locus_tag="KKO08_000201"
     CDS             complement(14016..15215)
                     /gene="lolC"
                     /locus_tag="KKO08_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBD6316613.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15477..16550
                     /locus_tag="KKO08_000202"
     CDS             15477..16550
                     /locus_tag="KKO08_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBD6316614.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16678..20124
                     /gene="mfd"
                     /locus_tag="KKO08_000203"
     CDS             16678..20124
                     /gene="mfd"
                     /locus_tag="KKO08_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBD6316615.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20267..21229
                     /gene="ldtC"
                     /locus_tag="KKO08_000204"
     CDS             20267..21229
                     /gene="ldtC"
                     /locus_tag="KKO08_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBD6316616.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21312..21524)
                     /locus_tag="KKO08_000205"
     CDS             complement(21312..21524)
                     /locus_tag="KKO08_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6316617.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21809..22441
                     /gene="comR"
                     /locus_tag="KKO08_000206"
     CDS             21809..22441
                     /gene="comR"
                     /locus_tag="KKO08_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBD6316618.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22503..23042)
                     /locus_tag="KKO08_000207"
     CDS             complement(22503..23042)
                     /locus_tag="KKO08_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD6316619.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23269..24573)
                     /gene="ndh"
                     /locus_tag="KKO08_000208"
     CDS             complement(23269..24573)
                     /gene="ndh"
                     /locus_tag="KKO08_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBD6316620.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24981..25523)
                     /locus_tag="KKO08_000209"
     CDS             complement(24981..25523)
                     /locus_tag="KKO08_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD6316621.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25546..26571)
                     /gene="nagZ"
                     /locus_tag="KKO08_000210"
     CDS             complement(25546..26571)
                     /gene="nagZ"
                     /locus_tag="KKO08_000210"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBD6316622.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26582..27406)
                     /gene="thiK"
                     /locus_tag="KKO08_000211"
     CDS             complement(26582..27406)
                     /gene="thiK"
                     /locus_tag="KKO08_000211"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBD6316623.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27387..28028)
                     /gene="lpoB"
                     /locus_tag="KKO08_000212"
     CDS             complement(27387..28028)
                     /gene="lpoB"
                     /locus_tag="KKO08_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBD6316624.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28043..28420)
                     /locus_tag="KKO08_000213"
     CDS             complement(28043..28420)
                     /locus_tag="KKO08_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBD6316625.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28423..28782)
                     /gene="hinT"
                     /locus_tag="KKO08_000214"
     CDS             complement(28423..28782)
                     /gene="hinT"
                     /locus_tag="KKO08_000214"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBD6316626.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            28913..29094
                     /locus_tag="KKO08_000215"
                     /pseudo
     CDS             28913..29094
                     /locus_tag="KKO08_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29127..31312
                     /gene="fhuE"
                     /locus_tag="KKO08_000216"
                     /pseudo
     CDS             29127..31312
                     /gene="fhuE"
                     /locus_tag="KKO08_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31372..32805)
                     /gene="ptsG"
                     /locus_tag="KKO08_000217"
     CDS             complement(31372..32805)
                     /gene="ptsG"
                     /locus_tag="KKO08_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBD6316627.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33100..33897)
                     /locus_tag="KKO08_000218"
     CDS             complement(33100..33897)
                     /locus_tag="KKO08_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD6316628.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33908..34912)
                     /gene="holB"
                     /locus_tag="KKO08_000219"
     CDS             complement(33908..34912)
                     /gene="holB"
                     /locus_tag="KKO08_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBD6316629.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34909..35550)
                     /locus_tag="KKO08_000220"
     CDS             complement(34909..35550)
                     /locus_tag="KKO08_000220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBD6316630.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35540..36562)
                     /gene="yceG"
                     /locus_tag="KKO08_000221"
     CDS             complement(35540..36562)
                     /gene="yceG"
                     /locus_tag="KKO08_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBD6316631.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36565..37374)
                     /gene="pabC"
                     /locus_tag="KKO08_000222"
     CDS             complement(36565..37374)
                     /gene="pabC"
                     /locus_tag="KKO08_000222"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBD6316632.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37494..38735)
                     /gene="fabF"
                     /locus_tag="KKO08_000223"
     CDS             complement(37494..38735)
                     /gene="fabF"
                     /locus_tag="KKO08_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBD6316633.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38822..39058)
                     /gene="acpP"
                     /locus_tag="KKO08_000224"
     CDS             complement(38822..39058)
                     /gene="acpP"
                     /locus_tag="KKO08_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD6316634.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39269..40003)
                     /gene="fabG"
                     /locus_tag="KKO08_000225"
     CDS             complement(39269..40003)
                     /gene="fabG"
                     /locus_tag="KKO08_000225"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBD6316635.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40016..40945)
                     /gene="fabD"
                     /locus_tag="KKO08_000226"
     CDS             complement(40016..40945)
                     /gene="fabD"
                     /locus_tag="KKO08_000226"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBD6316636.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40961..41914)
                     /gene="fabH"
                     /locus_tag="KKO08_000227"
     CDS             complement(40961..41914)
                     /gene="fabH"
                     /locus_tag="KKO08_000227"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBD6316637.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41982..43052)
                     /gene="plsX"
                     /locus_tag="KKO08_000228"
     CDS             complement(41982..43052)
                     /gene="plsX"
                     /locus_tag="KKO08_000228"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBD6316638.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43133..43306)
                     /gene="rpmF"
                     /locus_tag="KKO08_000229"
     CDS             complement(43133..43306)
                     /gene="rpmF"
                     /locus_tag="KKO08_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBD6316639.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43358..43879)
                     /gene="yceD"
                     /locus_tag="KKO08_000230"
     CDS             complement(43358..43879)
                     /gene="yceD"
                     /locus_tag="KKO08_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBD6316640.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44078..44662
                     /gene="yceF"
                     /locus_tag="KKO08_000231"
     CDS             44078..44662
                     /gene="yceF"
                     /locus_tag="KKO08_000231"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBD6316641.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44774..45733)
                     /gene="rluC"
                     /locus_tag="KKO08_000232"
     CDS             complement(44774..45733)
                     /gene="rluC"
                     /locus_tag="KKO08_000232"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBD6316642.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46306..49491
                     /gene="rne"
                     /locus_tag="KKO08_000233"
     CDS             46306..49491
                     /gene="rne"
                     /locus_tag="KKO08_000233"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBD6316643.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49687..50640)
                     /gene="flgL"
                     /locus_tag="KKO08_000234"
     CDS             complement(49687..50640)
                     /gene="flgL"
                     /locus_tag="KKO08_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBD6316644.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50652..52294)
                     /gene="flgK"
                     /locus_tag="KKO08_000235"
                     /pseudo
     CDS             complement(50652..52294)
                     /gene="flgK"
                     /locus_tag="KKO08_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52360..53301)
                     /gene="flgJ"
                     /locus_tag="KKO08_000236"
     CDS             complement(52360..53301)
                     /gene="flgJ"
                     /locus_tag="KKO08_000236"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBD6316645.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53301..54398)
                     /gene="flgI"
                     /locus_tag="KKO08_000237"
     CDS             complement(53301..54398)
                     /gene="flgI"
                     /locus_tag="KKO08_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBD6316646.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54410..55108)
                     /gene="flgH"
                     /locus_tag="KKO08_000238"
     CDS             complement(54410..55108)
                     /gene="flgH"
                     /locus_tag="KKO08_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBD6316647.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55161..55943)
                     /gene="flgG"
                     /locus_tag="KKO08_000239"
     CDS             complement(55161..55943)
                     /gene="flgG"
                     /locus_tag="KKO08_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBD6316648.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(56081..56832)
                     /gene="flgF"
                     /locus_tag="KKO08_000240"
                     /pseudo
     CDS             complement(56081..56832)
                     /gene="flgF"
                     /locus_tag="KKO08_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(56852..58060)
                     /gene="flgE"
                     /locus_tag="KKO08_000241"
     CDS             complement(56852..58060)
                     /gene="flgE"
                     /locus_tag="KKO08_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBD6316649.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(58085..58780)
                     /gene="flgD"
                     /locus_tag="KKO08_000242"
     CDS             complement(58085..58780)
                     /gene="flgD"
                     /locus_tag="KKO08_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBD6316650.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(58792..59195)
                     /gene="flgC"
                     /locus_tag="KKO08_000243"
                     /pseudo
     CDS             complement(58792..59195)
                     /gene="flgC"
                     /locus_tag="KKO08_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59199..59615)
                     /gene="flgB"
                     /locus_tag="KKO08_000244"
     CDS             complement(59199..59615)
                     /gene="flgB"
                     /locus_tag="KKO08_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBD6316651.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59770..60429
                     /gene="flgA"
                     /locus_tag="KKO08_000245"
     CDS             59770..60429
                     /gene="flgA"
                     /locus_tag="KKO08_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBD6316652.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60505..60798
                     /gene="flgM"
                     /locus_tag="KKO08_000246"
     CDS             60505..60798
                     /gene="flgM"
                     /locus_tag="KKO08_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBD6316653.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60803..61219
                     /gene="flgN"
                     /locus_tag="KKO08_000247"
     CDS             60803..61219
                     /gene="flgN"
                     /locus_tag="KKO08_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBD6316654.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61259..62794)
                     /gene="murJ"
                     /locus_tag="KKO08_000248"
     CDS             complement(61259..62794)
                     /gene="murJ"
                     /locus_tag="KKO08_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBD6316655.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62961..63827)
                     /locus_tag="KKO08_000249"
     CDS             complement(62961..63827)
                     /locus_tag="KKO08_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD6316656.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63829..64476)
                     /locus_tag="KKO08_000250"
                     /pseudo
     CDS             complement(63829..64476)
                     /locus_tag="KKO08_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
     gene            complement(64487..65071)
                     /gene="rimJ"
                     /locus_tag="KKO08_000251"
     CDS             complement(64487..65071)
                     /gene="rimJ"
                     /locus_tag="KKO08_000251"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBD6316657.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65307..66515
                     /gene="mdtH"
                     /locus_tag="KKO08_000252"
     CDS             65307..66515
                     /gene="mdtH"
                     /locus_tag="KKO08_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBD6316658.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66579..67226
                     /gene="grxB"
                     /locus_tag="KKO08_000253"
     CDS             66579..67226
                     /gene="grxB"
                     /locus_tag="KKO08_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBD6316659.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67360..67920
                     /locus_tag="KKO08_000254"
     CDS             67360..67920
                     /locus_tag="KKO08_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6316660.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            68026..69072
                     /gene="pyrC"
                     /locus_tag="KKO08_000255"
     CDS             68026..69072
                     /gene="pyrC"
                     /locus_tag="KKO08_000255"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBD6316661.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69146..69391
                     /gene="dinI"
                     /locus_tag="KKO08_000256"
     CDS             69146..69391
                     /gene="dinI"
                     /locus_tag="KKO08_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBD6316662.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69681..69935
                     /gene="bssS"
                     /locus_tag="KKO08_000257"
     CDS             69681..69935
                     /gene="bssS"
                     /locus_tag="KKO08_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBD6316663.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            70050..71168
                     /gene="solA"
                     /locus_tag="KKO08_000258"
     CDS             70050..71168
                     /gene="solA"
                     /locus_tag="KKO08_000258"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBD6316664.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71216..71329
                     /locus_tag="KKO08_000259"
     CDS             71216..71329
                     /locus_tag="KKO08_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBD6316665.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71590..72156
                     /locus_tag="KKO08_000260"
     CDS             71590..72156
                     /locus_tag="KKO08_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBD6316666.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72160..72735
                     /locus_tag="KKO08_000261"
     CDS             72160..72735
                     /locus_tag="KKO08_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBD6316667.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72777..73829)
                     /locus_tag="KKO08_000262"
     CDS             complement(72777..73829)
                     /locus_tag="KKO08_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBD6316668.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            74054..74974
                     /gene="lpxL"
                     /locus_tag="KKO08_000263"
     CDS             74054..74974
                     /gene="lpxL"
                     /locus_tag="KKO08_000263"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBD6316669.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75146..75343
                     /locus_tag="KKO08_000264"
     CDS             75146..75343
                     /locus_tag="KKO08_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBD6316670.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75367..>75876
                     /locus_tag="KKO08_000265"
     CDS             75367..>75876
                     /locus_tag="KKO08_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD6316671.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVS"
BASE COUNT        18496 a        20069 c        18764 g        18547 t
ORIGIN      
        1 tatctgtcgt gcaatagatc tatttcttgc agttccatta ttacaggctc aagaagcgtt
       61 attaatgccg tggcaattct tgatttttgt ttgtcgcgtt cattgtcgcc aagtgtttca
      121 agccatatgc gcaatatttc cagcatgttt tcactgtgag aaagtgcaag aaatgcgcgg
      181 tctattgttt cgtggtaaat atcacgcata ttaatccccg tccgtcgttt ttcttaaaat
      241 aacctctgtc acgaaatcag cataatcggc ggcgatattc agtacatcaa gcccgttgat
      301 ttatattctc tcgtggaaag taagaaaaaa gccgctctaa taagttctgg cattgacgaa
      361 agcgcatcag ccgcatcatc aggaacgccg gaaaattcct gtttcaggga attaaaacga
      421 tcatcacgca taaccccccc cattttcaca atcggcaaca agaatatttt tagcgtcatt
      481 cagtgaccgc gttgcgatgt atcggataca ttccagggcg aactgtgtcc ggtctttctg
      541 ttcttctccc tgcgcaagct ctgccgtgca ttcaatatca ataagcgcgt gcatcagcgt
      601 agtgagtgcg gcggctgctg cgttcggtgt ggttttattg tgcataggtt tccccctgac
      661 gaatacgggc ggcgaataca gcaacacaac cggacgggca acggctacgc gcttcgcgtt
      721 ccgtccaagc ggttacgtgg atgatttggg attctccggc actcagtgcc agaaaacgcc
      781 acacaaaggc cgtttgtgtg tgtacaaggt gtggtatatg atttacggca accataacgg
      841 ctcctagttt acgttgttgg ttagaagccc cgttactgct cctacagtgc ggggctttgc
      901 tttttgtgtt gattgtgtgt aatgtgtcac tacacataaa tacattacct gaggtgtaat
      961 taacgtgtca acacacaaaa acgaaaggcg tggtaatcct ccgttccagt tccgtttaga
     1021 tcctgactta cgggaaaaga tggaaaaggc acaacaacag gacggcgatg attctcttgc
     1081 tgcctggata aagcgaataa tccgcaaaga attacagcaa cgcggcatag aacccaaagg
     1141 ataaaaacta tcagcgccgt ggtgtgaggt actacggcgc attgctttac aggcgcacac
     1201 aatgaccaac gaagaatcaa ccgaaaaaac atcacctgca cgaaaaagac gacgcagaaa
     1261 gaaagagcat gaatcagaac gattcgcgcc gtgttctttt gcccttgaga agtaccttaa
     1321 agaatactca tcaaaggaga gaacttcgca gttatggcaa cgcactgaac cagactgatt
     1381 gcattgccca ccagcctgat agcggctatc atccccgtgc ttatgtttgg gatcacacac
     1441 acaaacggcg cagcgggtta tctgttcaga aaggcggctc catttcgggg ccgctttttt
     1501 tatgcctgaa aaaccccaat tttgtggttt tccagtttca ccagggcgaa cgaatccccg
     1561 cccacgttct ggcgtatatt caatcttcat ggttatagct ctgtgttcag atgattgatg
     1621 tgtggcggct gcgtgccgcc agcgtgatta atgaactgcc ttgcagctat ccttccaggc
     1681 cagaacctcg gataaagacc agccaacgga acgaccgcca agtttacgac gtgatgggaa
     1741 ttgtccggcc ttttccaggc ggtagcggca tgagcggcta aggcctgtta gcttttcgca
     1801 ttctttttca cgtataaacc gatcagtgct taacactatt gccccctttc gtttcttaaa
     1861 gagttatttc gtgttctatt gcgttgggat gtgtctgatt gtgtcaggat gattcagagt
     1921 tggcaaatgt tgaggccgca tggtttacag aaagaggaat aatcaggata aaatccttta
     1981 aattcatgtt aatacaaagg cataaaatat tgtttcatgc cttttttctc gctatttaaa
     2041 gagtgattcg ctagtgtata aaaaatcagc aacacattaa aaatcagcta tttataaatc
     2101 tgtacacttt ttcacctctt gttcgtagtt gttccgcttt gttgctcatt gttgcacgtt
     2161 gtatctgttc gcatatccag tatgcgcata ctgaaaaaac acgaaaaaaa ttattttctt
     2221 ctggctactg gtagcgtggt tacgttttca tgtgttcccg ccagtatccc taaccgctcc
     2281 gtccacatat ccagcgcatt gcgtttagca tccagataac gggaatgatt ataaactcgc
     2341 tgcattcctg gcatctggtg gcctgtaagc tgctccacga cgtgaggatc cacgcctaaa
     2401 tcgttcagca tcgttgtaaa ggtgcgccgg atgtcatgta gtgaccagtg agggtgattt
     2461 agcctcctgt gcgctaatct gccgtactgc gacacgcttg tttcctgttt cacttccccc
     2521 agcaataagc ccgtgtgcct gttctgctcc accagctgcg tgacgaacgg cagtattgct
     2581 tccggtatgg gccggaatat tgccaccttc gttttgctgt gttctttcgg cacggtccag
     2641 agcatttcgg taaaatccca ctcgctgatc tccgataacc tcagttctac cgtccggcat
     2701 ccgaacacaa tcaggaggcg gattaacgcg atgtagtagg gggagaatat ttttttgtcc
     2761 agtgcctgca ataattcgcc cagttctttg gtgcttaaga cacgctcgct tatatccggt
     2821 tttttcccaa cgtccgccac actcatatca tcaagaacgt tgctgattgc atagcgccgc
     2881 ctccggcaga acttaagcgc ctgtttgcac gtctgtagca agaatccggc agtaacaggc
     2941 gttcgctttg ccacctggtc aaaacaggcc agccagtgcc gtagctcgca tttatccagc
     3001 ggcatagcac caatgtgctg tattacgtga ttattaaggc gctttttcag ggcggcataa
     3061 tccacgcggt tttcctttgc gtacgactca agccagtagg tgagcgcatc gccaaccgtt
     3121 accggcttta acgcttcctg tacggtgtaa ttaagctcat gacgtggatt ttcccctctg
     3181 ccagccatgc gcgacactgg gcggcttttt ccctggctga tttcaggttc agatcaggat
     3241 aatttcccag cttaatgcgt tccggtggtg ccccccttcc cgttccggcc ctgtaagtga
     3301 aataccaggt taaaaggcca ctggttgaat gcctgacgct caggtttcca ccgtcattaa
     3361 gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct cagctgtgta tcgctcagtt
     3421 tgttaagtgc tctgctcata attttgattc cggtcatcac aattaactac accattaact
     3481 acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc tggaacaagg aaaatcacaa
     3541 atctgtttat aatcaaaaac ataatgaaca atctgaaaca ttatgaaacg gcaaaaaaca
     3601 ctaattgata aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt
     3661 gcagatacca gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat
     3721 tttcttgaac gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt
     3781 attgacccga tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt
     3841 cgactggtca gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac
     3901 gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg cagtaaacca ctggcatgag
     3961 ccgaccgccg cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg
     4021 atgatttctt tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg
     4081 tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa
     4141 cagcactgcc cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa
     4201 gagctggagc ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg
     4261 ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc caaatacgcg ggaattaatt
     4321 agcggatttg ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatccg
     4381 gatgttccac ctcgtgctca tcctgcggat gttctgccgc aagagatgga taaactgcgt
     4441 gagatgatgc tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt
     4501 atatcccagt cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat
     4561 gaaatctacg atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta
     4621 ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg
     4681 gcactggatg cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg
     4741 gaagatccgt cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc
     4801 gaagggtaag tttggattta agccggatgc agcatcacat gccgcatccc ttttcgctta
     4861 cttccgttgc gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc
     4921 ttccagaatc acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg
     4981 gcaaggtaat cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag
     5041 ttccggttca atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg
     5101 ctcaaccact ttctcgcgca tattgtagta actgtcttca atcaccaatt caatgtagca
     5161 atcaggatgt aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc
     5221 ttcaaactgt ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt
     5281 gcctttcatg ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc
     5341 atccgccgga acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc
     5401 tttcgccgtg cccggatgaa cattgttacc gacaattttt atattgaccg atgcggcgtt
     5461 gaagttttca aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc
     5521 atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa
     5581 ggcgacgcga atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat
     5641 gatttctgca atacctgctt tgtcatcagc acctaacaag gttttaccat cggtggtaat
     5701 caacgtctga cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc
     5761 gccgataccc agcgcaatat cgccaccgcg atagttttca acaatttgcg gattaacatt
     5821 tttgccgctg caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc
     5881 gccagggacg ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt
     5941 gataagcccc atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc
     6001 cgtgctggga acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa
     6061 aaatcgctca agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag
     6121 aagcaagaaa agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta
     6181 tcagcattac cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt
     6241 caagcggttt gccgtagaat taccgccctc attaagagtc accaaggtgg ttaaccacaa
     6301 accccgcatc ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa
     6361 caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt
     6421 aaagaggtca ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt
     6481 ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt
     6541 gattccggac gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc
     6601 tatgtgaaca ctgttttcca aagctatgca cttttccccc acatgaccgt gttcgaaaat
     6661 gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg
     6721 gaagccctgc ggatggtgca gctggaaacc ttcgctcaac gtaaaccgca tcagctctct
     6781 ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg ttaacaagcc tcgtctgttg
     6841 ttgctggatg agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag
     6901 ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt tcgtgactca cgaccaggaa
     6961 gaagcactca ccatgtcgga caggattgtg gtgatgcgcg atggtcgcat tgagcaagac
     7021 ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt ttgttgccgg gttcattggc
     7081 gaaattaata tgtttaacgc cacggtgatc gagcgactcg acgagcagcg cgtacgcgcc
     7141 aacgttgaag gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa
     7201 ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac
     7261 gctgaagggc tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg
     7321 gttgttgaac tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgat
     7381 cctgactttg accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag
     7441 gtcgtactgg ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt
     7501 cggttggctt gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt
     7561 gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct
     7621 gctcgatccg ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac
     7681 cctcgcctgc ctggtgctgg gctatccatt tgcctggttt ctggcgaagc tgccacacaa
     7741 ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc tggaccaact cattgattcg
     7801 tatctacggg ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg
     7861 gctgggcgtt atcgacacac caatccgtat catgttcacg cccagtgcgg tgattatcgg
     7921 tctggtttac attctgctgc cgtttatggt gatgccgctg tactccagta tcgaaaaact
     7981 ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat
     8041 ccgcatcatt attccactga cgatgccggg gattattgcc ggatgtctgc tggtgatgct
     8101 gccagcgatg ggcctgttct atgtgtccga cctgatgggc ggtgcgaaaa acctgctgat
     8161 cggtaacgtc atcaaggtac agttcctcaa tattcgtgac tggccgtttg gtgcagccac
     8221 cagcattacg ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg
     8281 tttgctgaat aagaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg
     8341 accgctatct acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac
     8401 agctcgcgct ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg
     8461 aacaacgaca gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg
     8521 acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgc
     8581 ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga tgtcaccaga tatcgtgatg
     8641 gcaatttcgc tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg
     8701 ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg tgacggtgta ttcgcgcctg
     8761 aaaggttttg acgtgcggat gctggaagcg gcgaaagatc tcggtgccag cgaatttacc
     8821 attctgcgaa aaatcattct gccactggca atgccagcgg tggcggcggg ctgggtgtta
     8881 agctttaccc tgtcgatgga cgatgtggta gtttcttcat tcgtcaccgg accaagttat
     8941 gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac
     9001 gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt
     9061 gctcgtgata aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc
     9121 tgctcgcggc gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca
     9181 cgctgtattt ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca
     9241 aagaaaccgg tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtacgcca
     9301 agctgaaaac gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg
     9361 ataaaatgcg taaagagggg atgatccaga agatcgacaa gtcgaagtta acgaacttca
     9421 gcaatctcga tccagacatg ctcaacaagc cttttgaccc gaataacgac tactccattc
     9481 cgtatatctg gggtgcgacg gcgattggtg ttaacggtga tgcggtggat ccgaaatctg
     9541 tcaccagctg ggccgatctg tggaagccag agtacaaagg cagcctgctg ttgaccgacg
     9601 atgcccgtga agtgttccag atggcgctgc gtaagctggg ctactccggt aacaccaccg
     9661 atccgaaaga gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtcgcag
     9721 cgtttaactc cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga
     9781 tctggaacgg ttctgctttc gttgcacgcc aggcgggtac gcctattgac gtggtgtggc
     9841 cgaaagaagg cggcatattc tggatggaca gcctggcgat cccggcaaat gccaaaaaca
     9901 aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg
     9961 ctgaaactat cggttatcca acgccaaacc ttgcggcgcg taagctgtta agtccagaag
    10021 tggcaaacga taagacgctc tacccggatg ctgaaaccat taaaaatggc gaatggcaga
    10081 atgacgttgg cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac
    10141 gttaattcag caagcgggcg gtggtattac cgcccgtttt ttagttagca aggatgtggc
    10201 taatgagtca ggacagtaaa gttttatccc gctctttcgt cggtattgtg gtccgcttgt
    10261 aggcctgata agcgtagcgc atcaggcaat gcttcctgct ttataatccc tccagcaact
    10321 tttcaacaaa ttctggcacc acctggcttg ccgggccgta atatttctcg gcaaattcat
    10381 taccaacctg actcggttca agattcagtt caacggtgtg agcaccatgt agtttggctt
    10441 cgtgtacaaa cccagccgcc ggatagacat gcccggaagt accaatggca ataaagacat
    10501 ccgccatcga caacgccata taaatttcat ccatgccgag tggcatttcg ccaaaccaaa
    10561 cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg gcatttatct tctggcgtaa
    10621 cgtctccggt ccagtcgaga acctgaccac tttgcgaaca acgaactttc agcaattccc
    10681 catgcatatg aatgacattg gtattacctg cgcgttcatg caggttgtct atattctgcg
    10741 ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt agccagcgca agatgcgcgg
    10801 cgttaggctg aatttctggc tgctgcagct gtcgacgacg ggcgttataa aacgcttgca
    10861 ccagttcagg atcgcgatcg aaaccttccg gcgttgccac atcttcaacc cgatgttctt
    10921 cccacaggcc atctgcggcg cgaaaggtac gaatacctga ttccgcagaa attcctgccc
    10981 ctgtcagtac gagtactctt ggtttttcca ttgcttccgg caccacttta tctctgaaaa
    11041 aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt tttacgaaaa cgacttaacc
    11101 gatgaccccg acgcgacagc atagcaacct ctgttgttta atcggttaga tgtaggaagg
    11161 ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg ttcaatgcgc ggaacacgag
    11221 ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg cgttgtgatt gccgggaaat
    11281 tcgataagcc accaccaatg acgaccaggt cagggtcaac aatggtcagg atatttccca
    11341 gagaaaccgc taataaatcc agataacgct caacgtgcgc ccttgcctgc tcatcgcctt
    11401 gatcataaag cgcaataatt tcgggagcct gcaacggttg atgataatag tgttgataca
    11461 gccacgcaaa accgcgacca gacagataat tttcaatgca gccatgctga ccacagccgc
    11521 agcggcgtaa cgggaaatcc aaccccatca tggttaacgc atcaaccggc agacgcatat
    11581 ggccaaactc gccggtaatg tagcttttac cagtaatcgg tttgccgttg aaaaccagcc
    11641 cgccgccaac gccggtgccg agaattaacc ccatcaccag tggatattgc gtaaattcat
    11701 catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt atcaaggcgt acatcgcgat
    11761 caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct ggcagcgggg acattagcgg
    11821 catacagctt accatcttct gtttccggca ttcccggaat accgatgccg acagaacctt
    11881 tacagccaaa acgttggtca gcttcagcta ccagctcaca cactgcatct aaaaatgcgt
    11941 catagctgtc acgcggtgtc ggcacccgct tttcccactg caactgccga ccgctatcaa
    12001 acacgccaag cgcaattttt gttccaccaa tatcaaaccc gtaatacatt gccgctcctt
    12061 gttttaatgt actgccttta ctggccgcta aggactcgcg cagggtcaat attactggcg
    12121 cgccgcgccg gataccaact tgccaaaaga ctcagcaaca atgctgtgac cagtacgtag
    12181 aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa taaaataaat atcgctggag
    12241 aggaactgat gaccgatcag cttttcaatc cactcaataa tcggggtaag ttgcagtgaa
    12301 acaactacgc cgataaccac cccacacagg ctgccgaaca gccctgccag caatccatac
    12361 cagacaaaga tggcgcgaat taaaccatct ttcgccccca gcgttcttaa tactgcgata
    12421 tcgccactct tgtctttcac cgccatcact aaggtggaga cgatgttgaa acaggccacg
    12481 ccaatcacca gtaccatcgc cagatacata atggcgcgga tcatctggat atcgcgatac
    12541 atatagccgt aagtaccaat ccaactttta atataaacat agctgttggt cacttcaccc
    12601 gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca ttttaagggc aatacctgac
    12661 acgctggaac ccatatcaag atattgttgg gcatccgcca gcgggatcat ggcaaaactg
    12721 tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt gcaaacgcac acgttttggc
    12781 tgcatcagtt tatgctcggg attcgagttg gggatcatga tcgacaccca atcgccctgc
    12841 ttcactttca gcgcatccgc cacgcctttg ccgatgataa tttgctgttc gcccgcttta
    12901 aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg cgctcagacg ctgttcctgt
    12961 tgtgggttaa cgcccttcac ctggattgcg cgcagattcg ctccactttc caccagaccg
    13021 gtgaaattga tatacggcgc agcggcggca atacccggca ctttctgcac gttatccagt
    13081 gcttcctgcc agttagtcca tggctgattt accgcttcga tttcgccatg cggcaccacc
    13141 gccagaatgc ggttattcag ttcgcgttca aagccgttca tcgcgcttaa gccgacgatc
    13201 aataccgcca cgccgagggc aatgccaatg gtagaaatga cagagattag cgacaccatg
    13261 ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca ataacgataa aggcatcgcc
    13321 attactccgc ccccatcagg ctcagttccg ccgtcagacg tccatcacgc atttccagtt
    13381 ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac caccaggaaa gcggtgccct
    13441 gcaagcgatt caactcccca agcaactgga agatgctgtc ggcgttacgc gcatcgaggt
    13501 taccggtagg ttcatccgcc agtaccagac gcggattatt gaccagcgca cgggcaatcg
    13561 ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg gtgattcgca cgatgctcca
    13621 gccccaccgc ttttaacatc tccagtgcac agctgttgat ttcagcgggc tttttcttgc
    13681 caatcagcag tggcatagcc acgttttcca gggcagtaaa atccggcagc aggtggtgaa
    13741 actgataaat aaagcccagc ttctggttgc gcagttctgc tttcgccgct gaagacagtt
    13801 tgctcatcgg ctgaccgtta aagatcacat cgccggaggt tggcgtatcc agcccgccta
    13861 acaggtgcag caaggtactt ttaccggaac cagagctacc gacaattgcc atcatttcac
    13921 cttcgcccac gctgaaactg acattgagca gcacatcggt ttgcacactg ccttcctgat
    13981 agcgtttgca caggttgtcg cattgcaaca ggatcttatt cataacgtaa agcctcagcg
    14041 ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag acagcagcgc gatagccatc
    14101 gccaccagcg caataacaat gacctgtaaa ggttcgatag ccaccggcag cgccgcgcca
    14161 tcaagcagga cgccgattat cggcatcaga ttatttaact ggctggcaag cagtgcgcca
    14221 agcgccgctc cgaggattgc accgataatc ccggcgctag ccccttgcac cataaagacc
    14281 atcatgattt gtcgcggagt taagccttgc gtttgcagga tcgcgacttc gccctgcttc
    14341 tccatcacca tcagccccag cgaggtaata atgttaaacg ccgcaacagc gacaatcagg
    14401 ctcagcagta agcccatcat atttttttcc atgcgtacgg cctggaacag ctcgccttta
    14461 cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt tttgctgact taaagagtca
    14521 actctcagcg gctcatccag ccacaaacgc cagccggtaa tattggctgc cggataacgc
    14581 atcagacgcg atgcatcctc aatattcacc agcatttcat agccatcgac ttcactgttg
    14641 gcggcgaaag tgccaatcac attgaacagg cgctgacttg gaatacgccc catcggcgtg
    14701 aactggctgg cagatggcac catcacgcgg atttgatcac cgcgattgac gcccaactgt
    14761 gaggcaagtt gttcgccgag gatgacatta tatttccccg gctcgaggtc agtttgtttc
    14821 acattgacca gatacggcgt tagtggatct ttttgcgccg ggtcgatacc gagcatcacc
    14881 ccgaccgcca cgctgcgcgc gctttgcagt accacatcac cggtagtaat aggtgcgacg
    14941 cgattaacgc cgtccagttt gactgccgtt tccgggagtt gctgcgggtt aagagagcca
    15001 tgctcagaag agagaattgc ctgtggcatc aggccaagga tgttgttttg cagctcgcgc
    15061 tcaaagccgt tcatcactga caatactgtg accagcgcca tcaccccgag ggtaatgccg
    15121 atggtagaaa gccaggagac gaaacgaccg aagcgatcca ctgcacgccc acgcatgtaa
    15181 cgcaggccaa taaatagagc gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa
    15241 agacacgagt atataagcga aagcaagatg ggtaaacgtc tgaactgtaa gtgatcgtca
    15301 gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat tgccatgttt tatcatcaat
    15361 cttgccgcct gatcttaatc tgaaaaatca tccaatacag acagataccc attacacctc
    15421 gggtcataac cagcaggatc aaccgcatgt aaattaaaaa agagcgtgtt gttctgatga
    15481 aacaaaaaga gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt
    15541 atcactcggt cattaccttt tatccgcata tgaccacttt tcagcatccg ttatcggaag
    15601 tcctgagcaa atgctggatc tatttcaatc tttaccttgc cccctttcgt atgccggttt
    15661 ttttctttat atccggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt
    15721 gtctcgataa acggatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt
    15781 ggctggcgtt aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct
    15841 ccaatgccgc ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca
    15901 ccagcttgtg gtatctgtat gcgttaattg tctatttcgt ggtatgtaaa atttttagcc
    15961 gtctggcgct gccactattc gccttgtttg tactgctgag tgtggcggtt aatttcgttc
    16021 ccacgccgtg gtggggaatg aacagtgtga tacgcaattt gccttattac agccttggcg
    16081 catggtttgg cgcaacaata atgacctgtg ttaaagaggt gccgttgcgc cgccatctgc
    16141 tgatggcttc tttgcttacc gttctggcgg tcggtgcctg gttgtttact atctcgctgc
    16201 tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt ttatcagtac gagcaacgat
    16261 tcggtatgcg ctccaccagc ctgctgaatg tgattggctc caacaccatt gctatctaca
    16321 ccacccatcg cattctggtt gaaatattca gcttaactct gcttgcgcaa atgaacgcag
    16381 cacgctggtc gccgcaagtc gaactgacac tcctgctggt ttatcccttt gtcagtttgt
    16441 tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc acagcgtgca ttcagcgatc
    16501 tgttgttctc cccgccttca ctgcccgctg ccgtcagtta ctctcgttaa accatcaccc
    16561 cagccggggc gattcaattt gctaaaccat gtcgattacg gataatgaac agcattgctt
    16621 atcagagata tgcccccata tgttgaggca tatcctaacg agaatctgac aaccgttatg
    16681 cctgaacaat atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag
    16741 ttaaccggcg cagcctgtgc aacgctggta gcggaaattg ccgaacgtca cgccggtccg
    16801 gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag
    16861 ttcaccgatc agatggtgat gaatctggcg gactgggaaa ctcttcccta cgacagtttt
    16921 tcgcctcatc aggacattat ctcctcgcgc ctttccaccc tttaccagct accgacgatg
    16981 cagcgtggca tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccacacagt
    17041 tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta
    17101 cgaacccaac tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa
    17161 tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt
    17221 cttgatttct ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga cagccagcgc
    17281 acgctggagg aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gaccgataaa
    17341 gcggcaattg aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg
    17401 gaacatattt accagcaagt gagtaaaggc acattacctg ctgggatcga gtactggcag
    17461 ccattgttct tcagcgaacc actgccgccg ctgttcagtt atttccctgc caataccttg
    17521 ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt tccaagctga cacgctggcg
    17581 cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt tgccaccaca atcgctctgg
    17641 ctgcgggtgg acgagctctt ctcagagctg aaaaactggc cccgagtgca gctaaaaact
    17701 gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt tccagaaact gccagacctg
    17761 gccgttcagg cgcaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc
    17821 gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg
    17881 ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcgaccgt
    17941 gggcgttatc tgatgattgg cgctgccgaa catggttttg tcgataaggc gcgtaatctg
    18001 gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg cccgccgtcg tcaggattct
    18061 cgccgcacca tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag
    18121 ccggtggtcc atctggagca cggcgtcggt cgctatgccg gaatgaccac gctggaagct
    18181 ggcggcatta ctggcgagta cctgatgctc acctatgcca acgacgccaa actgtatgtt
    18241 ccggtgtcgt cactgcatct gattagccgt tacgcaggtg gcgcggaaga aaacgccccg
    18301 ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc agaaagcggc ggaaaaagtg
    18361 cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc
    18421 gcgtttaaac acgatcgtga gcagtatcag ttattctgcg acagcttccc gtttgaaacc
    18481 acgccggatc aggcgcaggc cattaatgcg gtacttagcg acatgtgtca gccgctggca
    18541 atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc
    18601 gctttcctgg cagtagataa ccacaagcag gtagcggtgt tggtgcctac cacccttctt
    18661 gcgcagcagc attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa
    18721 atgatctccc gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa
    18781 gggaaaatcg atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa
    18841 gatttaggcc tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc
    18901 attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta ctgcaacgcc gatcccacgt
    18961 acgctgaata tggcaatgag cggaatgcgt gacctgtcga ttatcgccac gccgcccgcc
    19021 cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca gcctggtggt ccgggaggcg
    19081 atcctgcgtg aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac
    19141 attcagaaag ccgccgaacg gctggcagaa ctggtgcctg aagcacggat tgccatcggt
    19201 cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt
    19261 ttcaacgtgc tggtttgtac caccattatc gaaaccggga tcgacatccc gacagccaac
    19321 accattatca ttgaacgtgc ggatcacttc ggtctggcgc agctgcacca gttacgcggt
    19381 cgcgtcggac gttcacatca tcaggcatat gcatggctgc tgacgccgca tccaaaagcg
    19441 atgactaccg atgcacaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggggca
    19501 ggttttgcgc tggcaacgca cgatctggag attcgcggcg cgggtgaact gcttggcgaa
    19561 gaacaaagcg gttcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac
    19621 gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa
    19681 acagaagtcg agctgcggat gccgtcgcta ttgccagatg atttcatccc tgacgtgaac
    19741 acgcgtctgt cgttctacaa acgtattgcc agcgccaaaa cggaaaacga actggaagag
    19801 atcaaagtcg agcttatcga tcgcttcggc ctgctgccag atccggcgcg taccctgctg
    19861 gatattgccc gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat
    19921 gagaaaggcg gggtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt
    19981 ggtttgctgc aaaaacagcc gcagcattac cgccttgatg gtccgacgcg cctgaaattt
    20041 attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa
    20101 ctggaagaga acgcgatcgc ttaatgtaaa tcgggggcgt ttctgcgccc ccatctgcaa
    20161 catttacaaa ttctttgcac tttcctgcac tatccgacac tgtcaccatc cataattcag
    20221 gcttatctgt ttattacaat aaccttatat ttattatgta tttttggtga tgatcaaaac
    20281 gcgtttttct cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc
    20341 ggcaaaagcc aacacctggc cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa
    20401 atttcatgtg gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt
    20461 cggctttctc gctctgttac aagctaaccc cggcgttgat ccttacgtac cgcgcgcggg
    20521 cagcgtgtta acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt
    20581 gatcaacatt gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt
    20641 gtatccaata ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac
    20701 cgtttcagac aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta
    20761 taaagcacag ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg
    20821 ccatcatgcg attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc
    20881 cgatttcggc attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat
    20941 caaaacactc tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat
    21001 aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga
    21061 gaagattgat gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt
    21121 taaagatgca gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg
    21181 gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa
    21241 aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg
    21301 agggttaatg cttatttata aattactgct gttccatgga gggtattcgg accggttaca
    21361 gaagtaatac ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc
    21421 agcgatccca gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct
    21481 ggcgttgact gaacttcgac agccgcaaag ttggcaaatg acatggagct taaaatcgcc
    21541 gcagcgatga ggtttttacg tttttcataa tagtggcctt atgcagatga atgacgtcgc
    21601 aagaaattac ttaacgatcg ataactaaat gatagatgtg atctggatca catacaagat
    21661 attttttata acaatcatta attaatttaa aaatctttaa atttcagtca aatagaaagc
    21721 aattattttt atgggatctg gactggtgaa cggaggtgat tatttttata ataacctttc
    21781 attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa
    21841 aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt
    21901 gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag caaccggagc
    21961 caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc gtgccgttct
    22021 cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa
    22081 aagcgtggag tctgcgctgg ctgactattt tgcggccatc gccaactgct ttaccagcaa
    22141 agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc cagattcagg
    22201 agatatagcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt
    22261 tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cacatctggc
    22321 agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct
    22381 ggaaaaactg atgcagattg ccggaacgac tttgcgttta tggcccgaac tggtgaaata
    22441 agtcgcaaaa aaggcccctc agcctgagcg aatgaggggc caaattgcag agtacagcct
    22501 tgttatactt tgttattcaa aatcagttgc ccgttactat ctagcgggat ctgcgtaccc
    22561 ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa
    22621 ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa
    22681 gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc acccagcgcc
    22741 cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc tatcacgcca
    22801 ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga
    22861 tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg
    22921 gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag actggccact
    22981 gccgctacgc ccaacgcagc tgcgacacca atcccgatac ccgccaacat tgatttattc
    23041 acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag aaaagcagct
    23101 atcagacaga agatgaaaag cgcgggaatg actggaaata cttaaggatt cggaggagtc
    23161 tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt
    23221 gtccagggcg gataaggcgt ttataccaca tccgccagtg tacgtcgatt aatgcaactt
    23281 caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
    23341 gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat
    23401 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga
    23461 gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt
    23521 accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg
    23581 agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
    23641 gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg
    23701 gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
    23761 taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
    23821 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc
    23881 cgcagtagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt
    23941 tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgatcgc
    24001 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
    24061 cactttgcca tttgcgccca ggttggcaga gtatttcagg aacagattca gcatctcctg
    24121 gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg
    24181 cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt
    24241 acgttccgga accaacagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt
    24301 cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc
    24361 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
    24421 cagcagtggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
    24481 cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
    24541 gacaatcaca atctttttca atggcgtagt caacgtgacc cccttaaatt tattaaccaa
    24601 ttgttaatta atggttatta acatagctct taattaacaa caggttacag caatgaaaat
    24661 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
    24721 caagttttgt tgcttaaaag ataggtaact ttaccggggg agatcaaaaa atagagttta
    24781 ttgtttatta tttgagcaaa ttcggaaggg taatataggg tgaagcgtat atcgagggcg
    24841 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    24901 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    24961 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    25021 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    25081 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    25141 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    25201 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    25261 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    25321 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    25381 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    25441 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    25501 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctgcttt
    25561 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    25621 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    25681 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    25741 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    25801 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa aacccagttc
    25861 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    25921 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    25981 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    26041 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    26101 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    26161 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    26221 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    26281 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    26341 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    26401 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    26461 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    26521 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    26581 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    26641 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    26701 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    26761 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    26821 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    26881 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    26941 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    27001 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    27061 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    27121 ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    27181 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    27241 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    27301 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    27361 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    27421 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    27481 ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    27541 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    27601 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    27661 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    27721 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    27781 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    27841 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    27901 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    27961 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    28021 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    28081 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    28141 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    28201 cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag
    28261 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    28321 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    28381 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    28441 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    28501 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    28561 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    28621 cacgtcgttc acagtcggaa tgagaatatt cggaatgatc agaatatgcg ttggcgcttg
    28681 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    28741 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    28801 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    28861 ttaaacctgc tctttcaatc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc
    28921 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    28981 tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcat ttgcatttgc
    29041 agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga
    29101 cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
    29161 atcaagtcat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
    29221 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
    29281 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
    29341 agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag
    29401 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
    29461 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
    29521 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata
    29581 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
    29641 gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag
    29701 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
    29761 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
    29821 actcatggct ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg
    29881 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
    29941 gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
    30001 gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
    30061 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
    30121 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
    30181 tgctggttgg gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt
    30241 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
    30301 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
    30361 cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
    30421 tttcccacaa gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa
    30481 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
    30541 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
    30601 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
    30661 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
    30721 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
    30781 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
    30841 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga
    30901 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta
    30961 tcttgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
    31021 aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa
    31081 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
    31141 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
    31201 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat
    31261 cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
    31321 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
    31381 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
    31441 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
    31501 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
    31561 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
    31621 tgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
    31681 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag
    31741 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
    31801 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
    31861 gtggatgatg tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat
    31921 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcacggtt
    31981 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    32041 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    32101 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    32161 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    32221 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    32281 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
    32341 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    32401 gaaacggtta aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt
    32461 atccgccaag tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    32521 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    32581 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    32641 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    32701 cgcggaaccg acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag
    32761 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    32821 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
    32881 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
    32941 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    33001 atatgtcaca atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac
    33061 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    33121 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    33181 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    33241 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
    33301 tcgatccagg gggacataac gcgcagcatc tcgcagttgc tccgcattac ggaaggtcac
    33361 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    33421 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    33481 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    33541 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
    33601 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
    33661 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    33721 gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac
    33781 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    33841 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    33901 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
    33961 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt
    34021 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
    34081 ccagttcggc gaccagtccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc
    34141 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    34201 gattaagggc cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct
    34261 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
    34321 gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    34381 gtgaaagcca agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac
    34441 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
    34501 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    34561 cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt
    34621 cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg
    34681 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    34741 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    34801 catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat
    34861 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    34921 ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    34981 ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg
    35041 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    35101 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa
    35161 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc
    35221 gccctgatac gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc
    35281 cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    35341 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    35401 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    35461 cagttgctcg agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    35521 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    35581 gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    35641 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    35701 ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    35761 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    35821 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    35881 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    35941 gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    36001 ctttcaccat tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat
    36061 acatcaaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    36121 cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac
    36181 gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg
    36241 cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    36301 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa
    36361 cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg
    36421 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    36481 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    36541 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    36601 atataacgtt gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat
    36661 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac
    36721 ttcgacaagc tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc
    36781 gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa
    36841 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    36901 gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat
    36961 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc
    37021 gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg
    37081 tccgctgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    37141 cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa
    37201 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa
    37261 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    37321 tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc
    37381 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    37441 gctaagaaaa aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc
    37501 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    37561 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    37621 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    37681 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    37741 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    37801 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg
    37861 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    37921 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    37981 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    38041 agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    38101 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    38161 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg
    38221 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    38281 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    38341 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    38401 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    38461 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    38521 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    38581 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    38641 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    38701 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat
    38761 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    38821 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    38881 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    38941 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    39001 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    39061 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    39121 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    39181 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    39241 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    39301 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    39361 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    39421 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    39481 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    39541 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    39601 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    39661 aaacagatga aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta
    39721 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    39781 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    39841 ctttgccgtt ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag
    39901 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    39961 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    40021 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    40081 caatgcgttt cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta
    40141 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    40201 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    40261 caacagaaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    40321 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt
    40381 ttgcgccagc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    40441 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    40501 cttcacacgc tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg
    40561 tgccttccgg tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat
    40621 cagcgaaatc aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg
    40681 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg
    40741 cagtcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct ggcccctgct
    40801 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    40861 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    40921 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag
    40981 agccccaagt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    41041 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    41101 ggcgatccag cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac
    41161 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    41221 tattcgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    41281 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg
    41341 gcagcgtcag caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg
    41401 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    41461 cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    41521 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac
    41581 aggctgctgc aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac
    41641 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    41701 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    41761 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    41821 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    41881 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    41941 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    42001 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    42061 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    42121 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    42181 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    42241 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    42301 gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg
    42361 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    42421 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc
    42481 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    42541 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    42601 acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc
    42661 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    42721 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    42781 agggccatac gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca
    42841 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    42901 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    42961 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    43021 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    43081 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    43141 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    43201 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    43261 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    43321 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    43381 atacggcaaa tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg
    43441 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    43501 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    43561 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    43621 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    43681 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    43741 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    43801 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    43861 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    43921 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    43981 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    44041 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    44101 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    44161 acctgaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    44221 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    44281 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    44341 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    44401 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    44461 tcacctaagc gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc
    44521 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga
    44581 ccctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    44641 gaaaaacccg ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat
    44701 cggtgcagac ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    44761 ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat
    44821 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    44881 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    44941 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    45001 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    45061 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    45121 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    45181 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    45241 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    45301 ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    45361 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    45421 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    45481 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    45541 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac
    45601 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    45661 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    45721 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    45781 tatgcttata tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt
    45841 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    45901 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    45961 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    46021 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    46081 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    46141 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    46201 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    46261 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    46321 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    46381 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    46441 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    46501 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    46561 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    46621 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    46681 tatctggttc tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc
    46741 gatgaccgta ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg
    46801 cttatcgtgc gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc
    46861 ttccgcctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    46921 ctgattcatc aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat
    46981 atcggcgaaa tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct
    47041 gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    47101 ttcagccact accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg
    47161 ccatccggtg gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac
    47221 tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa
    47281 gccgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    47341 gacttcatcg acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    47401 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    47461 gaaatgtccc gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg
    47521 cgctgctccg gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    47581 ttgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    47641 ccaatcgctt cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt
    47701 caggacggtg ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac
    47761 gtgttgcgcg tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg
    47821 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa
    47881 caacctgcac tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa
    47941 cctgccgcga ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct
    48001 gcacaacctg ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt
    48061 gaagaagcca aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa
    48121 caggatcgtc gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc
    48181 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    48241 gcacagcagc aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa
    48301 gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat
    48361 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    48421 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    48481 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    48541 gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg
    48601 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    48661 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    48721 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    48781 atgccgttga ccgtagcgtg cacgtctccg gaattggcct ctggcaaagt ctggatccgc
    48841 tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta
    48901 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc
    48961 gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg
    49021 caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag
    49081 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa
    49141 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    49201 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    49261 gcagcagaag ttgaaacggt ggccgcggtt gaaccagaga tcaccgttga gcataaccac
    49321 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    49381 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    49441 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    49501 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatgtttg aatcaacata
    49561 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc
    49621 cgccccggcg atcccagagc gggcagacaa aacataccca gtttcatgat atgttttaaa
    49681 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    49741 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    49801 atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaaggaatc
    49861 cagcgactcc agtttgttca gctgcgtgcc taattccgcg cgaacggtaa ggacattatt
    49921 cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga tttctttatc
    49981 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    50041 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    50101 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgctt
    50161 aatactttct gttccgccaa cataatcacc gttagcctca ctaaacggcg cagtctctgt
    50221 tttataaccg gcaaaaatgt agcgcccatt accgtcagtg gtgttcgcca gattcagcaa
    50281 ctggtcacga agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt
    50341 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct
    50401 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgtgcca gcgtgtactg
    50461 gctgttttgc gcctgtgcct gggagagaac aacagcttgt gatgcagcaa tgggatcgtc
    50521 tgaagggtta acgactcgct tacccgtcga catctgctcg ccgtacttca tccattctgc
    50581 ctgagaattg gtgatgccgc gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    50641 tttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    50701 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    50761 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    50821 gtggtgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    50881 taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc
    50941 agggcctgac cgttgcggtt gtcgctatca cccgcatctt cttcgctcgc catcgcgatt
    51001 ttcgcttcgt cggtgattaa tacatccatg ttgacgatgg cgtcacttac tggtttcagc
    51061 gtgaagctgt cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    51121 actttaccgt tggcatctgg tgtcgccgta aaagtggtat tgctggcaag gcgggtgacc
    51181 tgccactgat tattatcgaa cgagattttg taatctgtcg ccagtaccac ggaggcatca
    51241 gttaccgtgg caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc
    51301 ttaccgatag taaagaaatc ttcaccagca tcgccgttag catcaaatcc ggctttgtgt
    51361 tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    51421 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    51481 tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg
    51541 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    51601 ccattggcat cgtgatgtta taagtaccgc catcctgaac gctgacttct acgccaacaa
    51661 tctggtttaa ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc
    51721 ctgcccccac gcctgtcagg cgcgagattt gatcgttcag gctggcaatt tgtttagcgt
    51781 aattgttgat ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc
    51841 gcagatattg atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg
    51901 cctggcgcgc cgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat
    51961 cctgcatctg tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca
    52021 tctgctcata gcgggcggtc aggccgctac tttgcgtctg cgcagcacgt aactggttgg
    52081 taataaacgt atcatactca cgctgcacac cagaaacgta gacgccattg ccaacccagc
    52141 cgccagcgcc caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg
    52201 caacgttata gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca
    52261 gtccgctcat ggcgttattg atcaagctgg acatggaggt tccttataag ccttcaatta
    52321 cagagtatta tcggcagcga cccgccggac ttgagttatt cagaacagat tatcaatgtt
    52381 catgctgtag gttttgctca ccttgtcgct tatcgatttc atctgctgga tcatgttggt
    52441 gagtttacgg gcatagtgag gatcggtagc atagcccgcg tcctgtagga cctgcgcccc
    52501 ctgttccgca ctcgcggcgg tcgtcacggc ggcgtagcgc gggttacgtg ttaacagccc
    52561 aacgtaatcc gacaatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt
    52621 cttcgcttcg ccgttttcat attcagtcgt ggtgatttca gtgacttgcc ctttccagtt
    52681 gccagaggct ttgacaccaa acaggttata gcttggctcg ccgttttcgc ggcggatttg
    52741 ccgttgcccc caaccagatt caagcgcagc ctgagcgagg atcaaatgat gtggcacacc
    52801 gctttgctgg cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctctactgtc
    52861 acccggcagc gaatcatcgt agttacgtgg tacggccttt tgcaccagct gcgaaagcgt
    52921 ctggttttga taacgcacca cggtttcgag cgggaatttc atcggtgctg ctggcgtgga
    52981 ctcctctggc aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag
    53041 acctttgccc gccgtcatct gttgggcaat ttgctggtca tacatactgg tatacagacg
    53101 agtgtgctcg ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat
    53161 catctgcacg aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc
    53221 ttcgctcgct ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag
    53281 tagtttgctg tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt
    53341 tgcattgatt gcaaaataga catcagatcc atcggcgtag cgcccagcgc attgagcgca
    53401 cgcaccacgt tattgaggct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg
    53461 cgtaaatcga tctgcgtttg tggagtaaca acagtctgtc caccaccaaa cggtgtatct
    53521 ggctggctga cattggcctg acgattaact gttactgaga gatttccctg cgctaccgcg
    53581 cagctgtcga gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact
    53641 actttagcgt cctgcggggt gacattaacc tgcatattct ggatatcggc aaggaaacgg
    53701 acctgggaac tgttgccact tggtacgcgc acctgaatag tccgcgcatc taacgcggtg
    53761 gcgctgccat atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa
    53821 tcttcgtcgt taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca
    53881 cgttcaataa ccgcaccatt ggtgatccgt ccaccgttca gttggttaac ctgaacactg
    53941 ctaccgcctg cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac
    54001 acctgactgt caacgccctt aagcggtgtc atcaacaacg tacctccacg caagcttttg
    54061 gcattaccca tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga
    54121 agtgacgctg tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga
    54181 acggtaattc ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg
    54241 gttgtctggt caccagtgcc atccagcccc actaccaagc catagccaat cagtgagttt
    54301 tgccttaccc cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgta
    54361 accagtagaa gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg
    54421 acaggttaag gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat
    54481 tgcctacgta ttcaatacgc gcatccgcca cctgagtaga cggtacggta ttgctgccgc
    54541 tgatagtgcg tggattaacc acgcccgaga agcgaataaa ttcggtaccc tgattaatgg
    54601 caatctgttt ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca
    54661 ccgtcaacgt gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt
    54721 taccaccgga ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg
    54781 gcacagtatc aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt
    54841 tgctggcgct gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc
    54901 gacgatcttc aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag
    54961 aaccgttggc gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca
    55021 gcggcgtgga gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg
    55081 cataagtatg cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg
    55141 aacctgccac cggttatgcc ttagagttgc gtcagttttt gcagcatctg atcggtggtg
    55201 gacaccgctt tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct
    55261 tccgccacgt tgacgttaga cgtttcaaca tacccttgat acagcagtcc cgcgccgttc
    55321 aggcccggcg tgctttcgtt cggtgcaccg gaggattgcg tttcggtgta gaggttttcg
    55381 ccaatgctct ccagtccggt atcattcata aaggtggtga gattgagctg cccaacctga
    55441 accggagctg cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata
    55501 cttaacgcat tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc
    55561 agctgcccgt tctgatccac ctggaaagag ccgtcacgga tataggctga tgagccatct
    55621 ggcaacatta cctggaaaaa gccctgccct ttaatcgcga catctttgct gttattggtc
    55681 tgcgacagat tgccctggct gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg
    55741 atttgtaatc cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg
    55801 gtttgataaa gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg
    55861 tttgccaggt tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg
    55921 gcgatccata atgaactgat catataatgt cctgtagtgg attgctgaca agcagagcag
    55981 aagcacgcga cagtgtgaat ttcccctcac cctaaccctc tccccaaagg ggcgagggaa
    56041 tagtccgcgc gcttttattg caatgtcatc aatcgttcaa ttaactcatc gacagcagtt
    56101 ggttggcacg gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac
    56161 gccgcgcgct ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca
    56221 gaacccccga catcacacgc aaggttggat ctgcctgcag taccggccct cgcgtggcct
    56281 gggtttctgc gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg
    56341 ctttcaccag tttcagacgc cctactggcg caactgtatt tgccggatcg cccggattga
    56401 gcgccgagat tgtgccatcg gcagcaatcg tgatttccgc cccttccggc acagcaattg
    56461 gccccgcctc gcctatcacc ggatgcccct gaattgtcag ttgcccggtg ggatcaacct
    56521 gaatgctgcc attacgcgta tacccttcgc tgccgtcagc ggtctgcacg gccagccatc
    56581 ctgctgcaac gcaacgtcca gcgggcgcga ggtgtaatcc atcttgccgg gcgtcatgtc
    56641 tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg
    56701 cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc gtagaggcat tggccagatt
    56761 actggcggtt accgcctgtt gattcagcgt ctggctggct gctcccatcg cagtataaat
    56821 tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaacccgc gtgttgagga
    56881 tctggtcctg ggttttgatg gtctgggcgt tagacttata gttacgctgg gcaacgatca
    56941 tattgaccag ttctttactg agatcgacgt tggacgcttc cagcgcaccg ttggtcaggg
    57001 tgccaaagtt tcccgtcccg gctgtcccca acagcgccac gccagaagat tgcgtcgcag
    57061 accagacgtt gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc gccagtacaa
    57121 tctgccccag cagttgggtt tgttcgttgg aattgttgcc gacaaccgta ccgtcatcat
    57181 tgatttgata actcaccaga tcgcccggtt tgtagccatt ctgggtggtt gccacaatgt
    57241 tgttagcgcc ggtattttgc tgcatggagt tgaggaagct cagactaaac gtggcgggtt
    57301 ctgcaccgtt aattgcgccg gttgcgatat tattcgccat cgcaccatcc actaatgtgc
    57361 cattagcatt aaattccagt gttgtcgctg tcttcgcaat gctgtttgga tcactgctat
    57421 cctgggtgta gacctgccag ttattatccc cggtcttcac aaaatagacg ctcatgtcat
    57481 gagcattacc ctgactgtcg aaaacagtca ccgaaccttt tttgttatag ctatccgcat
    57541 tgctggcgct gaatggcgta acagtaggaa gcggatcact ggaattcagg ttgatctgca
    57601 tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt cgggatcgaa atattggtcg
    57661 gattcgcccc ttgctgaata gtcggcggcg taccggttac cgggtaaccc gtcagctgta
    57721 aaccttgcgt attcaacagg ttacggtttt catccagctt aaattgtccg ttacggctgt
    57781 agaacaccga accgttgctg tctaccagac ggaaaaaacc gttctggctg atagcaacgt
    57841 ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa
    57901 cttttacccc cagtcccact ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg
    57961 atttaaagcc gtaggtggcg gagttggcga tattgttgcc aataacatcg aggttggtgg
    58021 cggcagcgtt taatccgcta accgcttgag aaaaggccat gactgactcc tgaaatgtga
    58081 aggcttaaat tatctgccgt acttcgtcga gggtggtggt gccgtaagtg ccgagatcca
    58141 gcgtattacc gctgttgccg cggatcacac cctgcaccag agcaaactgc agcggctggg
    58201 caaccagttg tgtaccaccg ttactggcgc taatcgctac attgtaagaa ccgttcggcg
    58261 cagtgctgcc atcagtcaac gaaccgtccc aggtgaaact gtgaactccg gcggtcagtt
    58321 caccaatatc aatggtgcgc acaaccgcgc catttttatc ggtgatggtg gccgtaactt
    58381 tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt cgtggtcaca gccccttctt
    58441 cactgccggt tcccgcaaga acagttgtgc cggggatcat cacgccgtga ccgatcaggt
    58501 tactggcctg taacgactgg ctgttatcaa tctgtccgga aatagatccg agcgtggtat
    58561 tgagtttttc aatcccgctg accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt
    58621 tttccattgg attggtcggg tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac
    58681 tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact actgctggtg gtactgacgc
    58741 cggtatttgt cggatcggtg gtggttaccg caatggacat agctttctcc tttattgacc
    58801 gagcgtaagg gttttcagca tcatgctttt caccgtgttg agcacttcaa cattggcctg
    58861 atagctgcgt gacgccgaca tggtgttaac catctccccg acgacatcaa cgttcggcat
    58921 ttttacgtag ccctttgcat ctgccagcgg attacctggt tcataaacca gtttgtccgg
    58981 ggcctgactt tcttaacatc ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg
    59041 ttaacctgga ataccacctg ttttgcccga tatggctgtc catcaggacc ggtcacgcta
    59101 tcagcatttg ccagattact ggccgccacg ttcaggcgct gggactgggc agttaacgcc
    59161 gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct ctgtaaaacg
    59221 ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta ttgcaggctg
    59281 ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc
    59341 tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg cgccgggatg
    59401 tgttgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc acgttgcatg
    59461 acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg ggtatcggca
    59521 ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag cgcctcttgt
    59581 tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt atcaaaattc
    59641 tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc
    59701 gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta gaaatggctg
    59761 ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact gttcagtccg
    59821 ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg
    59881 ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact accgccatgc
    59941 gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg
    60001 gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac aggaaattat
    60061 gtggttgccg cgatgcctat tgtgcgggga ggaaagctgg aagctggcaa tgtcaaactg
    60121 aaacgtggac gcctggatac cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt
    60181 gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac ccagtttcgc
    60241 caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg tgatgggttt
    60301 agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg
    60361 cgcatggtat cgggacaggt agtcagcggc gttgttgatg cagatgggaa tattcttata
    60421 aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa
    60481 agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct gaagcctgta
    60541 agcaccgttc aaccgcgcga aaccaccgac gcgccggtaa cgaacagccg ggcggcaaaa
    60601 acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa
    60661 cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac
    60721 ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa
    60781 gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc agatgtccgc
    60841 tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct ctatggggca
    60901 gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc tggcgacgct
    60961 ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg ttgatattag
    61021 tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga atcaacataa
    61081 cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa tgttgaaacc
    61141 gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacacccc atcgcggcag
    61201 taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga atgcattgtt
    61261 acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt gcagcgaagt
    61321 acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag ggcatggtac
    61381 ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac atcaccagta
    61441 ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta aagattttct
    61501 gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga ccaatagaaa
    61561 gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat tgcgttaaaa
    61621 tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa taaaagccag
    61681 gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca attaacgccc
    61741 gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc gaaacggtta
    61801 acggacctga aagaatgccc aacgcaaccg cactcggcag tgccaacagg aaacaaagac
    61861 gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca aaacttttcg
    61921 acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag ggaaactcca
    61981 ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa
    62041 tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc ggtcccatct
    62101 gtttcaccac gcgcattgct ccagcatcgt ggaagttaat gcgcggcagg accagcatgc
    62161 cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg accgtaacag
    62221 cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa
    62281 tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg ttccacgtat
    62341 tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga aaggtaatct
    62401 ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca
    62461 ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg
    62521 tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt
    62581 tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca aagatacggc
    62641 gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa
    62701 agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac atggtcatcg
    62761 agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccgg acccacgggc
    62821 aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctagatt atggtgaatt
    62881 gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat cttgtcaacg
    62941 atacgttgcg ccagcactgc ctattcgccg gcggtttgcg gaactgtctg attttgcacg
    63001 cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt gctctgccaa
    63061 ccaggaatcg gtttattcac tacaccctgc ccgcgctcct cacgccattc gcgcatatcc
    63121 gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg acttccggca
    63181 cggcgatgca tacaagtggt gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac
    63241 agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc tttgccgccc
    63301 gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt gaaataaaga
    63361 tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttagcgaggc tgcggtggcg
    63421 agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac
    63481 gtcagttttt tccgcgccgc cagttccacc agcctttcag catcgcgcag attttctgcc
    63541 agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac cacgtcaaag
    63601 tggctggcgg tgctggagtg cacaaaaacc gcatcacaac tggcggcaag gctggataac
    63661 gaatcggcat aaggaatgcg ccagctgtca caaattggca gggctttggc gcgcgtaggc
    63721 gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa ccacgctttt
    63781 tgcgcaatgc cgcctaatcc cactacgccg atacgtaatt ttttcacttt aatctcccag
    63841 atgggccagc aacgaatcaa gacgttgttt cagttctgcc acttcgattt ccagggcttc
    63901 gacgcgggtc tataaatcac cgtcaaccat gttcgacata gccgttaccg ccggctgatc
    63961 ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg gttcgcgggc
    64021 cagacgcacc acaaaaggac catcttcgcg attcgccagt tgttccagcg tcgactccac
    64081 ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt
    64141 ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc tcagtttcag
    64201 atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat taccaaaacc
    64261 gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct gcacttcgga
    64321 ttcactcaga ttcatgaccg gtttacggtt cgttttctga ttacaggccg tgactacacc
    64381 attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca agcaaccaat
    64441 tacgcgagct tccaaggcag taagttgata tttcatgaga ttctccttag cggccgggcg
    64501 tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca
    64561 gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc
    64621 gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg cgctgcatat
    64681 aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat ttttgcccaa
    64741 tcgaataacc gagatagcag gcatgaaaag agccgcgaac aacattggaa aaattggcaa
    64801 cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct gaaccttgtt
    64861 tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa cagtggcttt
    64921 cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa taatccgcca
    64981 gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg gttaagcgca
    65041 cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc gtaacgtctt
    65101 taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc
    65161 cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac gttttgataa
    65221 aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc
    65281 tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta
    65341 aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc
    65401 tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc
    65461 tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca attgccgacc
    65521 gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc ggattcgcca
    65581 caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc tcgggactcg
    65641 gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac
    65701 aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccagtgcg gtcattggcg
    65761 cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc acaggggccg
    65821 ttttgtttgt gctatgtgcg gcgttcaatg cgtggttgtt accggcatgg aaactctcca
    65881 ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca
    65941 cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg ctgatgctgc
    66001 caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg tatgccattg
    66061 aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa aagcattttc
    66121 gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg atgccggtgg
    66181 gcatggtcag cggcctacaa caacttttca ccctgatttg tctgttttat atcgggtcga
    66241 tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg
    66301 gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt ggttatatcg
    66361 gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt ccgtggatga
    66421 tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt agtcagaaac
    66481 gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga tactcccccc tgactcctcc
    66541 atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac atttacgatc
    66601 actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat
    66661 tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt caaaaacagg
    66721 ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat atcgttcact
    66781 atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct gccattgaag
    66841 agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt tttgccaaat
    66901 cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac aagaaagagg
    66961 ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt aagaatatca
    67021 gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac
    67081 tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg gtagccggaa
    67141 tcaactggcc aagccgcgtt gctaattacc gcgataatat ggcgaaacag acacaaatca
    67201 atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt attcgcgggt
    67261 tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca tgctgcgttg
    67321 tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt tttatttgcc
    67381 gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca atacaccatc
    67441 accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa agatatcggt
    67501 ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca aattggtcgc
    67561 gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa ctccctgttc
    67621 ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa
    67681 aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca accggaaaaa
    67741 atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg caattacttt
    67801 aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa
    67861 aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt tacggattaa
    67921 tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc
    67981 tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac tgcaccatcc
    68041 caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga tggcgacatg
    68101 ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt aatgcccaat
    68161 ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg tattcttgac
    68221 gccgtacctg ccgggcacga tttcacccca ttgatgacct gttatttaac agattcgctg
    68281 gatcctaatg agctggagcg cggatttaat gaagccgtgt tcaccgctgc aaaactttac
    68341 ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc aatcatgccg
    68401 gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga agtgacacat
    68461 gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg
    68521 cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc
    68581 gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca gcatctgatg
    68641 tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg tttacccatc
    68701 ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg ttttaatcga
    68761 gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag cagttgcggc
    68821 tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag
    68881 atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca gttctatggc
    68941 ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt tgctgaaagc
    69001 atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt acgctggtcc
    69061 gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct gtataaataa
    69121 ccagtatgtt caacaggggg ctattatgcg aattgaagtc accatagcga aaacttctcc
    69181 attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta ttcagtatgc
    69241 gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata atttatcggt
    69301 tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg aaacgtggga
    69361 aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt ttgccggatc
    69421 gccccggttt ttttatttta cttacgaatt ttgagtacaa aatttctaca cttcctcaaa
    69481 ataattgcac aatatgctaa aaaaatatga atgacatgct gttttattgt tcatataaat
    69541 cttaaaagca gaaatttatc tgttcccccc tttaaaaatt cggtgaatac ccttacttat
    69601 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
    69661 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
    69721 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
    69781 agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca
    69841 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
    69901 gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
    69961 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
    70021 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
    70081 gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga
    70141 ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc
    70201 gccatgctta tggtgaaggc gaaaagtatg tcccgttggt cctccgcgcg caaacgctgt
    70261 gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta
    70321 acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc
    70381 aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg
    70441 tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg
    70501 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc
    70561 cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt
    70621 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc
    70681 tgcctgtcca gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg
    70741 tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt
    70801 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
    70861 cagcggatga acgtgttccg tttgcggaag tggccagtga tgggtcagaa gccttcccgt
    70921 tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg
    70981 ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc
    71041 tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
    71101 ccgattttgc gcaagaccaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct
    71161 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg
    71221 tcggctgttg cactatctca tcaataatat tcgcgagcat ttgatgcttt atctttttct
    71281 ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact
    71341 gacataaact gacaaatatt taatctccct ttcatttagc gtgaaaaatt gaatgatatt
    71401 attcctgctt ctacagcact caccagacat ctctgatgct gaacccacta aacttatgaa
    71461 gattctttca ttattaattc aaaattattg aaataaaaat aaatatcatt caatttgtta
    71521 tttttgtgtt gcaattcatt ctccgacacg ccattttctt tctctcgcaa attcgtattg
    71581 gatgcactta tgtcattcac aaatacccct gaacgctatg gtgttatatc tgcggccttc
    71641 cattggctaa gcgcaattat tgtctacggc atgtttgctt taggtctgtg gatggtcacg
    71701 ctcagttatt acgatggctg gtatcacaaa gcacccgaac tgcataaaag catcggtatt
    71761 ttgttaatga tggggctggt tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt
    71821 ccgctgccga gttattcgcc catgactcgt cttgccgcaa aagctggaca tctcgcattg
    71881 tacctattgc tgttcgccat cggcatcagc ggctatctga tctcaactgc cgatggtaaa
    71941 ccgatcagcg tttttggctg gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag
    72001 gcagactttg ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt actgtctgtt
    72061 atgcacggat ttatggccct gaagcatcat ttcatcgata aagacgacac tctgaagcgc
    72121 atgctgggaa agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt
    72181 ttaaccttcg cgtccctgat gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt
    72241 gacaaagaag gtcagcacgc ctttgttaac ttccgcatac agcatcttgg ctatagctgg
    72301 ttatatggca cctttaaaga tttcgacggt acttttacct ttgatgaaaa aaatccggct
    72361 gccgataaag tgaatgtgac aattaacacc accagcgtcg atactaacca cgccgaacgc
    72421 gataaacatc ttcgcagtgc ggattttctc aataccacaa aatatccaca ggcaacattc
    72481 acctccacca gcgtgaagaa agacggtgac gaactggata ttaccggcga tctgacgctg
    72541 aatggcgtaa ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca
    72601 tggggcggta aacgtgcagg ctttgaggcc gaaggcaaaa ttaagctcaa agacttcaat
    72661 atcaagacag atttaggtcc agcttctcag gaagtggatc tgattatttc agtggaaggc
    72721 gtacagcaga agtaagagtg aaatgttgcc ggatggcacg catccggcaa tgatatttat
    72781 tctgttggat caggaatacc cagtgttgta ttcagacgtc cacgagactt attaaagatc
    72841 ttattgccat tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt
    72901 tcttcgcagc aaatctcact acaacaacct ttgtattttt ccgcgcatac tggacactga
    72961 ataaacagca ggtggcagcc atcattttta cagttggtat ggctgtcgca cggcgcgccg
    73021 cactggtggc aatgcgcgat aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa
    73081 acaaaatttt tgccaataaa acgcaccggc aagccctgct cgcgcgcctt acgggcgtat
    73141 tcaataattc cgccctcgat atgccagact ttgttgaatc cgttatgttt catccaggca
    73201 ctggcctttt cacaacgaat gccgccggtg cagtacatga cgattttttt atctttatgt
    73261 gcctgcatca tctcgactgc ttttggcagc tgctcacgga aggtatctgc cggaatttcg
    73321 agcgcgtttt caaagtgccc cacttcatac tcatagtggt tacgcatgtc gataaatagt
    73381 gcatcgggat cgtcaagcat ggcgttcact tccgccgctt gcagatactc accaacattg
    73441 ctggcatcaa agtgaggatc gtcaataccg tcggcaacaa tgcgatcgcg taccttcatg
    73501 cgcagtaccc agaaggattt cccgtcatca tccaacgcga tattcaggcg taaaccctct
    73561 aaagccgggt cgaaggcata gagctgcgcg cgaaatgttt caacattgct cgcaggtacg
    73621 ctgatttgcg cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac attcagcgcg
    73681 gtaaacagct gatataaagc gtcacgggtc gccttaggat cggcgatgtg gaaatacttg
    73741 taaaacgaaa tggtggttcg cggttcgctc tcagccaaca ttttggcttt taacgcgtcg
    73801 ttggaaatgc ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat gggaaagtaa
    73861 aaatccgcgg catgatatag caattatcga taattaacat ccacacattt tacgctacat
    73921 ttgcgcatta aaaattattt gttatttaca agcgcggcaa tttcgcccag tcttcagcca
    73981 caaattttgg ttgcgggcga aaaaatgcga caatacatac aattgcccgt ataggttgaa
    74041 aaacaggatt gatatgacga atctacccaa gttctccacc gcactgcttc atccgcgtta
    74101 ttggttaacc tggttgggta ttggcgtact ttggttggtc gtgcaattgc cctacccgat
    74161 tatctaccgc ctcggttgtg gattaggaaa actggcgtta cgttttatga aacgacgcgc
    74221 aaaaattgtg catcgcaacc tggaactgtg cttcccggaa atgagcgaac aagaacgccg
    74281 taaaatggtg gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa ccggtatggc
    74341 gtggttctgg ccggaccgcc gaatcgcccg ctggacggaa gtgatcggca tggaacacat
    74401 tcgtgacgtg caggcgcaaa aacgcggcat cctgttagtt ggcatccatt ttctgacact
    74461 ggagctgggc gcgcggcagt ttggtatgca ggaaccgggg attggcgttt accgcccgaa
    74521 cgataatcca ctgattgact ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat
    74581 gctcgaccgc aaagatttaa aaggcatgat taaagccctg aaaaaaggcg aagtggtctg
    74641 gtacgcacca gatcatgatt acggcccgcg ctcaagcgtt ttcgtcccgt tatttgccgt
    74701 tgagcaggct gcgaccacga ccggaacctg gatgctggca cggatgtccg gcgcatgtct
    74761 ggtgcccttc gttccacgcc gtaagccaga tggcaaaggg tatcaattga ttatgctgcc
    74821 accagagtgt tctccgccac tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt
    74881 ggtcgaaaaa tgcatcatga tggcaccaga gcagtatatg tggttacacc gtcgctttaa
    74941 aacacgcccg gaaggcgttc cttcacgcta ttaaatctcc catgccggat gcttcagaat
    75001 ggcatccggc attaccacag caaatccccc tgatttagcg ataaaagctc tctggattgc
    75061 gccccctgga agtcgggcgc ataattagtg tgtttatctt ttcttcttat gttcaccgcg
    75121 cctggcgcac caacagcgga ttgctatgtc accctgtgaa aatgacaccc ctataaactg
    75181 gaaacgaaac ctgatcgtcg cctggctagg ctgttttctt accggggccg ccttcagtct
    75241 ggtaatgccc ttcttacccc tctacgttga gcagcttggt gttaccggcc actccgccct
    75301 gaatatgtgg tccggtattg tcttcagtat aatgggttcc taaacacatc attttaggta
    75361 ttgaatatga gacgggcaat cagctacatc agattttcaa gtgaacgaca actgaaaggg
    75421 gactctgtac gccgacaatc taagctggtt actgactggc ttgataagaa ccctgagttt
    75481 tacttggatt catccttgag ctttaaagat ttggggaaaa gtgcttttag tggtaagcac
    75541 ctgaaaggtg gtctaggtga ctttttaacc gctatcgaga aagggcttgt taaagctggt
    75601 gatacgctac taatcgagag tttagaccgt ctgtcacgtc aggatattga tattgcttct
    75661 gagctattac gccgtatctt aagggctggt gtagatgtag tcacactgag cgatggagaa
    75721 cattacacac gagaaagttt aaaagacccg ttagccctga ttaaaagcat tctgataatg
    75781 cagcgggcgc atgaggaaag tttgagaaag tctgagcgtg tccaagctgc atggaaccgt
    75841 aaaaaagaac tcatctccga aggtatcaag gtatct
//
LOCUS       DAECNO010000004        75551 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.004, whole genome shotgun sequence.
ACCESSION   DAECNO010000004 DAECNO010000000
VERSION     DAECNO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.004"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(380..586)
                     /locus_tag="KKO08_000266"
     CDS             complement(380..586)
                     /locus_tag="KKO08_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBD6316672.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            899..988
                     /gene="kdpF"
                     /locus_tag="KKO08_000267"
     CDS             899..988
                     /gene="kdpF"
                     /locus_tag="KKO08_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBD6316673.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            988..2661
                     /gene="kdpA"
                     /locus_tag="KKO08_000268"
     CDS             988..2661
                     /gene="kdpA"
                     /locus_tag="KKO08_000268"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBD6316674.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2684..4731
                     /gene="kdpB"
                     /locus_tag="KKO08_000269"
                     /pseudo
     CDS             2684..4731
                     /gene="kdpB"
                     /locus_tag="KKO08_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4740..5312
                     /gene="kdpC"
                     /locus_tag="KKO08_000270"
     CDS             4740..5312
                     /gene="kdpC"
                     /locus_tag="KKO08_000270"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBD6316675.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5305..7985
                     /gene="kdpD"
                     /locus_tag="KKO08_000271"
                     /pseudo
     CDS             5305..7985
                     /gene="kdpD"
                     /locus_tag="KKO08_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7982..8659
                     /gene="kdpE"
                     /locus_tag="KKO08_000272"
     CDS             7982..8659
                     /gene="kdpE"
                     /locus_tag="KKO08_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBD6316676.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8934..9038
                     /locus_tag="KKO08_000273"
     CDS             8934..9038
                     /locus_tag="KKO08_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBD6316677.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9349..11546
                     /gene="speF"
                     /locus_tag="KKO08_000274"
                     /pseudo
     CDS             9349..11546
                     /gene="speF"
                     /locus_tag="KKO08_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11543..12862
                     /gene="potE"
                     /locus_tag="KKO08_000275"
     CDS             11543..12862
                     /gene="potE"
                     /locus_tag="KKO08_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBD6316678.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12918..14558)
                     /gene="pgm"
                     /locus_tag="KKO08_000276"
     CDS             complement(12918..14558)
                     /gene="pgm"
                     /locus_tag="KKO08_000276"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBD6316679.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14584..15129)
                     /gene="seqA"
                     /locus_tag="KKO08_000277"
     CDS             complement(14584..15129)
                     /gene="seqA"
                     /locus_tag="KKO08_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBD6316680.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15314..16078
                     /gene="ybfF"
                     /locus_tag="KKO08_000278"
     CDS             15314..16078
                     /gene="ybfF"
                     /locus_tag="KKO08_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD6316681.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16218..16511
                     /gene="ybfE"
                     /locus_tag="KKO08_000279"
     CDS             16218..16511
                     /gene="ybfE"
                     /locus_tag="KKO08_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBD6316682.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16651..17181
                     /gene="fldA"
                     /locus_tag="KKO08_000280"
     CDS             16651..17181
                     /gene="fldA"
                     /locus_tag="KKO08_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBD6316683.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17391..17477
                     /gene="uof"
                     /locus_tag="KKO08_000281"
     CDS             17391..17477
                     /gene="uof"
                     /locus_tag="KKO08_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBD6316684.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17470..17916
                     /gene="fur"
                     /locus_tag="KKO08_000282"
     CDS             17470..17916
                     /gene="fur"
                     /locus_tag="KKO08_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBD6316685.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18000..18326)
                     /locus_tag="KKO08_000283"
     CDS             complement(18000..18326)
                     /locus_tag="KKO08_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316686.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18363..19769)
                     /gene="chiP"
                     /locus_tag="KKO08_000284"
     CDS             complement(18363..19769)
                     /gene="chiP"
                     /locus_tag="KKO08_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBD6316687.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20348..22012)
                     /gene="glnS"
                     /locus_tag="KKO08_000285"
     CDS             complement(20348..22012)
                     /gene="glnS"
                     /locus_tag="KKO08_000285"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBD6316688.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22215..24161)
                     /gene="nagE"
                     /locus_tag="KKO08_000286"
     CDS             complement(22215..24161)
                     /gene="nagE"
                     /locus_tag="KKO08_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBD6316689.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24494..25294
                     /gene="nagB"
                     /locus_tag="KKO08_000287"
     CDS             24494..25294
                     /gene="nagB"
                     /locus_tag="KKO08_000287"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD6316690.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25354..26502
                     /gene="nagA"
                     /locus_tag="KKO08_000288"
     CDS             25354..26502
                     /gene="nagA"
                     /locus_tag="KKO08_000288"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD6316691.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26511..27731
                     /gene="nagC"
                     /locus_tag="KKO08_000289"
                     /gene_synonym="nagR"
     CDS             26511..27731
                     /gene="nagC"
                     /locus_tag="KKO08_000289"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBD6316692.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27779..28531
                     /gene="nagD"
                     /locus_tag="KKO08_000290"
     CDS             27779..28531
                     /gene="nagD"
                     /locus_tag="KKO08_000290"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBD6316693.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28928..30592
                     /gene="asnB"
                     /locus_tag="KKO08_000291"
     CDS             28928..30592
                     /gene="asnB"
                     /locus_tag="KKO08_000291"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBD6316694.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            30971..31047
                     /locus_tag="KKO08_000292"
     tRNA            30971..31047
                     /locus_tag="KKO08_000292"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31005..31007,aa:Met,seq:cat)
     gene            31056..31140
                     /locus_tag="KKO08_000293"
     tRNA            31056..31140
                     /locus_tag="KKO08_000293"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31090..31092,aa:Leu,seq:tag)
     gene            31164..31238
                     /locus_tag="KKO08_000294"
     tRNA            31164..31238
                     /locus_tag="KKO08_000294"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31196..31198,aa:Gln,seq:ttg)
     gene            31273..31347
                     /locus_tag="KKO08_000295"
     tRNA            31273..31347
                     /locus_tag="KKO08_000295"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31305..31307,aa:Gln,seq:ttg)
     gene            31363..31439
                     /locus_tag="KKO08_000296"
     tRNA            31363..31439
                     /locus_tag="KKO08_000296"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31397..31399,aa:Met,seq:cat)
     gene            31488..31562
                     /locus_tag="KKO08_000297"
     tRNA            31488..31562
                     /locus_tag="KKO08_000297"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31520..31522,aa:Gln,seq:ctg)
     gene            31600..31674
                     /locus_tag="KKO08_000298"
     tRNA            31600..31674
                     /locus_tag="KKO08_000298"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31632..31634,aa:Gln,seq:ctg)
     gene            complement(31828..33003)
                     /gene="ubiF"
                     /locus_tag="KKO08_000299"
     CDS             complement(31828..33003)
                     /gene="ubiF"
                     /locus_tag="KKO08_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBD6316695.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33149..34573
                     /gene="miaB"
                     /locus_tag="KKO08_000300"
     CDS             33149..34573
                     /gene="miaB"
                     /locus_tag="KKO08_000300"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBD6316696.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34726..35766
                     /locus_tag="KKO08_000301"
     CDS             34726..35766
                     /locus_tag="KKO08_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBD6316697.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35763..36230
                     /gene="ybeY"
                     /locus_tag="KKO08_000302"
     CDS             35763..36230
                     /gene="ybeY"
                     /locus_tag="KKO08_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBD6316698.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36320..37198
                     /gene="corC"
                     /locus_tag="KKO08_000303"
     CDS             36320..37198
                     /gene="corC"
                     /locus_tag="KKO08_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBD6316699.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37223..38761
                     /gene="lnt"
                     /locus_tag="KKO08_000304"
     CDS             37223..38761
                     /gene="lnt"
                     /locus_tag="KKO08_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBD6316700.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39158..40066
                     /gene="gltI"
                     /locus_tag="KKO08_000305"
     CDS             39158..40066
                     /gene="gltI"
                     /locus_tag="KKO08_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBD6316701.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40236..40976
                     /gene="gltJ"
                     /locus_tag="KKO08_000306"
     CDS             40236..40976
                     /gene="gltJ"
                     /locus_tag="KKO08_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBD6316702.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            40976..41650
                     /gene="gltK"
                     /locus_tag="KKO08_000307"
     CDS             40976..41650
                     /gene="gltK"
                     /locus_tag="KKO08_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBD6316703.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41650..42375
                     /gene="gltL"
                     /locus_tag="KKO08_000308"
     CDS             41650..42375
                     /gene="gltL"
                     /locus_tag="KKO08_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBD6316704.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42493..43428
                     /gene="rihA"
                     /locus_tag="KKO08_000309"
     CDS             42493..43428
                     /gene="rihA"
                     /locus_tag="KKO08_000309"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBD6316705.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43512..45173
                     /gene="hscC"
                     /locus_tag="KKO08_000310"
                     /pseudo
     CDS             43512..45173
                     /gene="hscC"
                     /locus_tag="KKO08_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45233..46684)
                     /gene="djlC"
                     /locus_tag="KKO08_000311"
     CDS             complement(45233..46684)
                     /gene="djlC"
                     /locus_tag="KKO08_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBD6316706.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46681..47388)
                     /locus_tag="KKO08_000312"
     CDS             complement(46681..47388)
                     /locus_tag="KKO08_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD6316707.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47490..48044
                     /locus_tag="KKO08_000313"
     CDS             47490..48044
                     /locus_tag="KKO08_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD6316708.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48054..49481)
                     /locus_tag="KKO08_000314"
     CDS             complement(48054..49481)
                     /locus_tag="KKO08_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBD6316709.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49478..50185)
                     /locus_tag="KKO08_000315"
     CDS             complement(49478..50185)
                     /locus_tag="KKO08_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD6316710.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50349..51326
                     /locus_tag="KKO08_000316"
     CDS             50349..51326
                     /locus_tag="KKO08_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD6316711.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51396..51878)
                     /locus_tag="KKO08_000317"
     CDS             complement(51396..51878)
                     /locus_tag="KKO08_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBD6316712.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52113..54695
                     /gene="leuS"
                     /locus_tag="KKO08_000318"
     CDS             52113..54695
                     /gene="leuS"
                     /locus_tag="KKO08_000318"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBD6316713.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54710..55291
                     /gene="lptE"
                     /locus_tag="KKO08_000319"
     CDS             54710..55291
                     /gene="lptE"
                     /locus_tag="KKO08_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBD6316714.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55291..56322
                     /gene="holA"
                     /locus_tag="KKO08_000320"
     CDS             55291..56322
                     /gene="holA"
                     /locus_tag="KKO08_000320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBD6316715.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56324..56965
                     /gene="nadD"
                     /locus_tag="KKO08_000321"
     CDS             56324..56965
                     /gene="nadD"
                     /locus_tag="KKO08_000321"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBD6316716.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            56989..57591
                     /locus_tag="KKO08_000322"
     CDS             56989..57591
                     /locus_tag="KKO08_000322"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBD6316717.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDIFATLRVLNSRAIGVENA"
     gene            57851..58168
                     /gene="rsfS"
                     /locus_tag="KKO08_000323"
                     /gene_synonym="rsfA"
     CDS             57851..58168
                     /gene="rsfS"
                     /locus_tag="KKO08_000323"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBD6316718.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58172..58639
                     /gene="rlmH"
                     /locus_tag="KKO08_000324"
     CDS             58172..58639
                     /gene="rlmH"
                     /locus_tag="KKO08_000324"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBD6316719.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58670..60571
                     /gene="mrdA"
                     /locus_tag="KKO08_000325"
     CDS             58670..60571
                     /gene="mrdA"
                     /locus_tag="KKO08_000325"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBD6316720.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60574..61686
                     /gene="mrdB"
                     /locus_tag="KKO08_000326"
                     /gene_synonym="rodA"
     CDS             60574..61686
                     /gene="mrdB"
                     /locus_tag="KKO08_000326"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBD6316721.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61697..62785
                     /gene="rlpA"
                     /locus_tag="KKO08_000327"
     CDS             61697..62785
                     /gene="rlpA"
                     /locus_tag="KKO08_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBD6316722.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62924..64135
                     /gene="dacA"
                     /locus_tag="KKO08_000328"
     CDS             62924..64135
                     /gene="dacA"
                     /locus_tag="KKO08_000328"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBD6316723.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64246..64509
                     /locus_tag="KKO08_000329"
     CDS             64246..64509
                     /locus_tag="KKO08_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBD6316724.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64610..65251
                     /gene="lipB"
                     /locus_tag="KKO08_000330"
     CDS             64610..65251
                     /gene="lipB"
                     /locus_tag="KKO08_000330"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBD6316725.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65510..66463
                     /locus_tag="KKO08_000331"
     CDS             65510..66463
                     /locus_tag="KKO08_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD6316726.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66672..67637
                     /gene="lipA"
                     /locus_tag="KKO08_000332"
     CDS             66672..67637
                     /gene="lipA"
                     /locus_tag="KKO08_000332"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBD6316727.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67738..67941)
                     /gene="tatE"
                     /locus_tag="KKO08_000333"
     CDS             complement(67738..67941)
                     /gene="tatE"
                     /locus_tag="KKO08_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBD6316728.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68070..68858)
                     /locus_tag="KKO08_000334"
     CDS             complement(68070..68858)
                     /locus_tag="KKO08_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBD6316729.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            68951..69334
                     /gene="crcB"
                     /locus_tag="KKO08_000335"
     CDS             68951..69334
                     /gene="crcB"
                     /locus_tag="KKO08_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBD6316730.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69387..69596)
                     /gene="cspE"
                     /locus_tag="KKO08_000336"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69387..69596)
                     /gene="cspE"
                     /locus_tag="KKO08_000336"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD6316731.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69771..70331)
                     /gene="pagP"
                     /locus_tag="KKO08_000337"
     CDS             complement(69771..70331)
                     /gene="pagP"
                     /locus_tag="KKO08_000337"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBD6316732.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70919..72304
                     /gene="dcuC"
                     /locus_tag="KKO08_000338"
     CDS             70919..72304
                     /gene="dcuC"
                     /locus_tag="KKO08_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD6316733.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(72345..72686)
                     /locus_tag="KKO08_000339"
                     /pseudo
     CDS             complement(72345..72686)
                     /locus_tag="KKO08_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73024..73533)
                     /locus_tag="KKO08_000340"
     CDS             complement(73024..73533)
                     /locus_tag="KKO08_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD6316734.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73696..73941
                     /locus_tag="KKO08_000341"
     CDS             73696..73941
                     /locus_tag="KKO08_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6316735.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73916..75547
                     /locus_tag="KKO08_000342"
                     /pseudo
     CDS             73916..75547
                     /locus_tag="KKO08_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18579 a        18983 c        19202 g        18787 t
ORIGIN      
        1 taaagtatat attactctct ttactatctg tttttattgc ggtagcgata ctaataacaa
       61 aattcggaat ttatttagat gctggaatta gaaaaaggga tgacggcttt ctcaccttac
      121 cctgaagttt cacgaaagtg tgtaaggaag tatgcaatct aactggagat ttctgatgat
      181 ctagtcgctt agagcgacaa tgaaggtgac cgcatcctgg gtagccctat gaaaggatac
      241 tattgaaacg tgatgctaaa acagtgatga aatgaataat acgatggaaa cgtcttccat
      301 gttaattatc atggaagacg ttgatgttga acacctaaac cagccccctg atacgaggct
      361 ggcttaagcg cgcaggcaat cagtaaaaat caccggttgc cttttcggcc tgatccatcc
      421 acaccggttt atcgctggtt ttcgaccaga cgcggtgcag gtagctgtaa aagcgggcgc
      481 ggtctttcca gaaaagcatg aaaggcagcg ccagaacgcc cgctgcaacc gcatatgtac
      541 ggcgtaaaaa gataagccat gcaggatatt ctctgtagag ttccatactt ttctccccct
      601 tttgtatcta cccggtgaat agcaccggaa aaatgaattt gtttatctga tgaaaatagt
      661 accgcctttt gtgtaatttt actactcatc cgaccactta tttttgctta ttgatggttt
      721 atttacattc acactgtaat taaattacac aaaagttaaa ttaatactaa ccatcagtta
      781 aatcatggcc tttgccattt ttatactttt tttacacccc gtccgcagat ttttgcgaaa
      841 tctttgcagc cagaattcta ctcttccggt atcactttta ggccactgga ggtgcactgt
      901 gagtgcaggc gtgatagccg gcgtgttgct ggtgttttta ttactgggtt atttggttta
      961 tgccctgatc aatgcggagg cgttctgatg gctgcgcaag ggttcttact gattgccacg
     1021 tttttactgg tattaatggt gctggcgcgt cctttaggca gcgggctggc gcggctgatt
     1081 aatgacattc ctcttccagg tacagcgggc gttgaacgta tactctttcg tttacctggc
     1141 gtctctgacc atgagatgaa ctggaagcaa tatctttgtg ccattctcgg cctgaacatg
     1201 ctggggctgg cggtgctgtt ttttatgttg ctcggtcagc actatctgcc gcttaatcca
     1261 cagcagttgc cggggctgtc gtgggatctg gcgctgaata ccgccgtcag ctttgtcacg
     1321 aataccaact ggcaatctta tagcggtgaa accacgttga gctatttcag ccagatggca
     1381 ggcttaacgg tacaaaactt tctttctgcc gccagcggga ttgcggtgat ttttgccttc
     1441 atccgtgcgt ttactcgcca gagcatgagc acactcggga atgcctgggt cgatctgcta
     1501 cgcatcacgt tatgggtgct agtccctgtg gcgttgttga tcgcactgtt ttttattcaa
     1561 caaggtgcgc agcaaaactt tctgccctat caggcagtga ataccgttga aggagcgcaa
     1621 cagctgttac ccatgggacc agtagcttct caggaagcga tcaagatgct cggtactaac
     1681 ggcggtggct tctttaatgc caactcgtcg catccgtttg aaaacccaac cgcactgacc
     1741 aacttcgtgc agatgctggc gatcttcttg atcccaacag cgctgtgctt tgcctttggt
     1801 gaagtgacgg gcgatcgccg ccaggggcgc atgttgctgt gggcgatgtc agtgattttt
     1861 gtcatctgcg taggcgtggt tatgtgggca gaagttcagg gtaatcctca tctgctggcg
     1921 ctcggcgcag acagcagcat caatatggaa ggtaaagaga gccgtttcgg cgtgctggtc
     1981 agtagcctgt ttgcggtcgt gactacggcg gcgtcctgtg gcgcggtgat tgcgatgcat
     2041 gattcgttta ccgctctcgg cggcatggtg ccgatgtggc taatgcaaat tggtgaagtg
     2101 gtgttcggcg gtgtcggttc tggtctttac ggcatgatgc tgtttgtcct gctggcggtg
     2161 tttattgccg ggctgatgat tggtcgtaca ccggaatatc tgggtaaaaa aatagacgta
     2221 cgcgagatga aactgactgc actggcgatt ctggttactc caacactggt actgatgggc
     2281 gcggcgttgg cgatgatgac cgacgccgga cgtagcgcca tgcttaaccc tagcccgcat
     2341 ggttttagcg aagtgctgta cgccgtgtcg tccgccgcta acaacaacgg cagcgccttt
     2401 gccggattaa gcgccaactc tccgttctgg aactgtttac tggcgttctg catgtttgtc
     2461 ggtcgcttcg gggtgattat cccggtgatg gcaattgccg gttcgctggt gagtaaaaag
     2521 agccaacccg ccagctccgg cacgctgcca acgcacggcc cgctgtttgt tggcctgtta
     2581 atcggcaccg tgttgctggt tggcgcactg acctttatcc ctgccctggc gcttggtccg
     2641 gtggcggaat atctctcctg atgatattga gtgagcactg aatatgagtc gtaaacaact
     2701 ggcgctattc gaaccaacac ttgtcgttca ggcgctgaaa gaagcggtga aaaaattaaa
     2761 cccgcaggcg caatggcgca atccggtgat gtttatcgtc tggatcggca gtctgctgac
     2821 cacctgtatt agcatcgcga tggcaagcgg tgcgatgcct ggcaatgcgc tgtttagcgc
     2881 ggcaattagc ggctggctgt gggtcaccgt actgttcgct aatttcgccg aggcgctggc
     2941 agaaggccgc agtaaagcgc aggccaacag tcttaaaggg gtgaaaaaaa ctgcctttgc
     3001 ccgcaagctg cgtgagccga aatatggcgc tgcggcggac aaagttcctg ccgatcaact
     3061 gcgtaaaggt gacatcgtgc tggtagaagc tggcgatatt atcccctgcg atggtgaagt
     3121 cattgaaggc ggcgcatcgg tcgatgaaag cgccatcacc ggggaatcgg caccggtgat
     3181 ccgtgaatcc ggcggcgatt ttgcctccgt caccggcggc acgcgtattc tttctgactg
     3241 gctggtgatt gagtgtagcg ttaaccccgg tgagacattt ctggatcgga tgatcgcgat
     3301 ggtggaaggc gcacagcgac gcaaaacacc gaacgagatt gccctgacta tcctgctgat
     3361 tgctctgacc atcgtctttt tactggcaac cgccacgctg tggccgtttt ccgcgtgggg
     3421 cggtaatgcc gtgagtgtaa cggtactggt ggcgctgctg gtctgtctga tcccaaccac
     3481 tattggtggc ctgttgtcag cgatcggcgt cgccgggatg agtcggatgc tgggcgcgaa
     3541 tgtgattgcc accagcggac gtgcagttga agcggcaggt gacgttgacg ttctgctact
     3601 ggataaaacc ggcaccatca cactcggtaa ccgtcaggcg tcggagttta tccccgcgca
     3661 gggcgtggat gaaaaaacgc tggctgacgc cgcacaactg gcttcgctgg ctgatgaaac
     3721 gccggaaggt cgcagtattg tgatcctcgc caagcaacgt tttaacctgc gcgagcgcga
     3781 tgtgcagtcg ctccatgcca cctttgtacc gtttactgcg caaagccgga tgagcgggat
     3841 caacatcgac aaccgcatga tccgtaaagg ttctgtcgat gccattcgcc gccatattga
     3901 ggctaacggc ggtcacttcc ctgccgatgt tgatcaaaaa gtcgatcagg ttgcgcgtca
     3961 aggagccacg ctgctggtgg tggtggaagg ttctcgtgtg ctgggcgtta ttgcgctgaa
     4021 agatatcgtc aaaggcggta ttaaagagcg cttcgcccag ctgcgcaaat gggcattaaa
     4081 acggtgatga ttaccggcga taaccgtctg actgccgccg cgatagctgc ggaagcgggt
     4141 gtcgatgatt ttctcgccga agcgacaccg gaggccaagc tggcattgat ccgtcagtat
     4201 caggcggaag gtcgtttggt agcgatgacc ggcgacagca ccaacgatgc tacggcgctg
     4261 gcgcaggcag atgtcgcggt ggcgatgaac tccggcaccc aggcggctaa agaggcgggc
     4321 aatatggtgg atctcgactc taacccgacc aagttgatcg aggtggtgca cattggcaaa
     4381 cagatgctga tgacccgtgg ctcgctgacc accttcagca ttgccaacga tgtggcgaaa
     4441 tacttcgcca ttattccggc ggcgttcgcg gcaacatatc cgcagttaaa tgcgctgaac
     4501 atcatgcgcc tgcattcgcc cgactccgcc attctcagtg cggtcatttt caacgccttg
     4561 attatcgtct ttttgattcc tctggcgtta aaaggcgtga gttataaacc gcttaccgct
     4621 tctgccatgt tgcgccgtaa cttatggatt tacggtctgg gtgggctgct ggtgccgttt
     4681 attggtatca aagtcattga tttactgcta accgtttgta gtctggtgtg aggtttacca
     4741 tgagtggatt acgtccggca ttatcgacat ttctctttct gttattgatt actggcggcg
     4801 tttacccgct gctgaccacc gcactggggc aatggtggta tccctggcag gccaacggtt
     4861 cgttgattcg tgaaggtgat acggtgcgcg gttcggcatt aatcgggcaa aattttaccg
     4921 gcaacggcta ttttcatggt cgcccgtcgg caacagcaga aatgccctat aatccacagg
     4981 cttctggcgg gagcaatctg gcagtcagta acccggagct ggataaacag atagccgcac
     5041 gcgttgccgc attacgggcc gctaacccgg acgccagcac gaacgttccg gttgaactgg
     5101 tgacggcgtc ggcaagcgga ctggacaata acatcacccc gcaagcggcg gcctggcaga
     5161 tcccacgcat cgcaaaagcg cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa
     5221 aatacagcca acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac
     5281 tcaatctggc actggataag cttgatgaat aacgaaccct tacgtcccga ccccgaccgt
     5341 ctgctggaac aaactgccgc gccgcatcgg gggaagctga aagttttctt cagtgcctgt
     5401 gcaggcgtcg ggaagacctg ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg
     5461 ctggatattg tggttggtgt ggtagaaacc catgggcgga aagataccgc cgccatgctt
     5521 gaagggctgg ctgttctgcc tctgaaacgc caggcgtacc gtgggcggca tatcagcgag
     5581 tttgatctcg atgccgccct cgcccgccgt ccggcgctga tcttaatgga cgaactggcg
     5641 cacagtaatg cgccaggttc tcgtcatcct aaacgctggc aggatatcga agaactgctg
     5701 gaagctggca ttgatgtttt cacaaccgtc aacgttcagc atctggaaag tctgaatgat
     5761 gtggtcagcg gcgtcaccgg aattcaggta cgggaaaccg tgcccgatcc ttttttcgat
     5821 gccgccgacg acgtggtgct ggtggacttg cccccggacg atctgcgcca gcggctgaaa
     5881 gaaggcaaag tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa
     5941 ggcaatctga tcgccctgcg cgaactggca ctgcgccgta ctgccgatcg cgttgatgag
     6001 caaatgcgcg cctggcgggg gcatcctggc gaagagaaag tctggcacac gcgcgacgcg
     6061 atccttttat gcatcggcca taacaccggc agcgaaaact ggtccgcgcg gcggcgcggc
     6121 tgacgtcacg gctggggagc gtctggcacg cggtgtatgt tgaaacccct gccctgcatc
     6181 gcttaccgga gaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg caggagctgg
     6241 gcgcggagac ggcaacactt tctgatccag cggaagagaa agcggtagtg cgttatgccc
     6301 gtgaacataa tctcggcaag attattctcg gtcgcccagc ctcgcgccgc tggtggcgtc
     6361 gggaaacatt tgctgaccga ctggcgcgca tcgcccccga tctcgatcag gtgctggtcg
     6421 cgcttgatga accacccgcc cgcacgatta acaacgcgcc ggatagccgc tcatttaaag
     6481 acaagtggcg ggtacagatt cagggatgcg tggttgccgc cgcgttatgc gccgttatca
     6541 ccttaattgc catgcagtgg ctgatggcgt ttgatgccgc caacctggtg atgctgtatc
     6601 tgcttggcgt ggtggtggcg ctattttatg gacgctggcc ttcggtggtt gccaccgtca
     6661 ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc gccgtctctg
     6721 atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg atcgggaacc
     6781 ttactgctgg cgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa cgcacacggc
     6841 acttatatga aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac atcgccgcca
     6901 ccagcgaaca atttattgcc tccacgtttc atgcccgcag tcaggtgttg ttgcccgatg
     6961 acaacggtaa attgcagccg ttaacacatc cgcaaggagt tacgccatgg gacgatgcca
     7021 tcgcgcagtg gagttttgat aaaggcctgc ctgcgggcgc gggcaccgac acgttacccg
     7081 gtgtaccgta ccagattttg ccgctaaaaa gcggcgagaa aacctacggg ctggtggtgg
     7141 tggagccggg gaatctgcgc cagttgatga tcccggaaca gcagcgcctg ctggagactt
     7201 ttacgctgtt agtcgccaat gcccttgagc ggctgacgct aaccgccagc gaagaacagg
     7261 cgcggatggc aagcgaacgt gaacagatcc gcaacgccct gctggcagcg ctttcgcatg
     7321 atttacgcac gccgcttacg gtgctgtttg gtcaggcaga aatcttaacg ctcgatctgg
     7381 caagcgaagg atcaccccac gcccgccagg ccagcgagat tcgtcagcat gtgctgaaca
     7441 ctacccgact ggtgaataat ctactggata tggcgcgaat tcagtccggc ggctttaatt
     7501 tgaagaaaga gtggttaacg ctggaagaag tagtcggcag cgcgctgcaa atgccggaac
     7561 cgggtttatc gtcgcccatc aatctttctc tgccagaacc gctgacctta atccacgttg
     7621 acgggctact ctttgaacgg gtgctgatta atctgctgga gaacgcggtg aaatatgcgg
     7681 gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg cgaaaatcta caactggatg
     7741 tctgggataa cggccccggt cttccgccag gccaggagca gacgatattt gataagtttg
     7801 ctcgcgggaa taaagagtcg gcggtgccgg gtgtcgggct tgggttggca atttgtcggg
     7861 cgattgtgga tgtacacggc ggcaccatta ccgcgttcaa ccgaccggaa ggtggtgcct
     7921 gttttcgtgt tacacttccc cagcaaaccg ctcctgaact tgaagacttt catgaggata
     7981 tgtgacaaac gttctgattg ttgaagatga acaggctatt cgtcgctttc tgcgcacggc
     8041 gctggagggc gacgggatgc gcgtctttga ggccgaaact ctgcaacgcg gcttgctgga
     8101 agcggcaacc cgtaagccag atttgattat tctcgatctc ggcctgcccg atggtgatgg
     8161 gattgagttt atccgcgacc tgcgccagtg gagcccggtg ccggtgattg tgctttccgc
     8221 acgcagcgaa gagagcgaca agatcgccgc gctggatgcc ggagcggatg attatctgag
     8281 taagccgttt ggtattggcg aattgcaggc ccgtctgcgc gtcgctttac gccgccactc
     8341 cgccaccgcc gcgcccgatc cgctggtgaa attttccggt gttaccgtcg atttagccgc
     8401 ccgcgtgatt caccggggtg aggaagaggt gcatctcaca ccaattgagt tccgcctgct
     8461 ggcggtgctg ctcaacaatg ccggaaaagt actcacccag cgccagctcc ttaaccaggt
     8521 gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg gacatctgcg
     8581 acaaaaactg gaacaggatc cggcccggcc tcgccacttc attactgaaa ccggtattgg
     8641 gtatcggttt atgctttgaa ttgttattca ataaattcgc ttaaatagtg attaatttat
     8701 tattaaacct gcgccgaata ttattatttg gcaggtttaa tttcttaacg caaatataaa
     8761 acacgaaatt acaacattta agtaacaaaa cagcatctaa tatggcgata ttcataatta
     8821 atgatttatt atctggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat
     8881 ttcatggata gaataactct accttcaact gacacagcaa gcggtaaagg taaatggaaa
     8941 ataataaccg cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc
     9001 gtaatagttt tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc
     9061 aacgtacagg tctccctgac tttaagcatt acaggttaat acctgtattc ctcggtgctc
     9121 atatactgct aaccctttta aactctaaat aattcgagtc gcagcacttg caacttgagg
     9181 tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca aaaagcaaac
     9241 tactgattta tttatcagcg gtggagcttt gctttttttc cgacgtgatc gatttctcct
     9301 ttgagaaatt gaggacctgc tattacctga aataaagaga tgaataaaat gtcagaatta
     9361 aaaattgccg ttagccgttc ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat
     9421 attgacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcaa tgatgttgaa
     9481 cgtggaaaac tcgataaaat agacgctact ggctatggca ttcctgtttt tattgcaacg
     9541 gaaaatgaag aacgtgtacc agcagaatat ctgccacgta tttctggtgt cttcgagcat
     9601 tgcgaatcac ggaaagagtt ttatggacgt cagttagaaa ccgctgctag ccattatgaa
     9661 acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgtcaatca gggcaatagc
     9721 gcttttgact gcccgggtca tcagggaggt gagtttttcc gtcgtcatcc ggcagggaat
     9781 cagttcgtgg agtacttcgg agaaacgctg ttccgttccg acttatgcaa cgcagacgta
     9841 gcaatgggcg atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcggca
     9901 aaagtgttta atgccgataa aacatacttc gttctaaatg gcacttcatc ttctaacaaa
     9961 gtggttttaa acgccctgct aacaccgggt gatctggtgc tgtttgatcg caataaccac
    10021 aaatctaacc accacggagc gttgctacag gctggtgcaa caccggttta tctggaaacg
    10081 gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga agaaagttac
    10141 ctgcgtgagc tgatcgcgga agtcgcaccg cagcgggcaa aagaggctcg tcctttccgc
    10201 ctcgctgtga ttcagttagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg
    10261 gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt tggctatgag
    10321 caatttatcc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa tgagaacgat
    10381 ccgggtattc tagttacgca atccgtacat aaacagcagg ctggtttttc tcaaacttca
    10441 caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc
    10501 atgaacaacg cctttatgat gcacgcctcc accagcccgt tctatccgct gtttgccgca
    10561 ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt
    10621 gtggtaaatg gcattaatgc ccgcaaactg atcctcgata actgtcagca tattcgtccg
    10681 ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg
    10741 gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcattcttt tgaaggctat
    10801 gcagagaatc aatactttgt cgatccttgc aaactgttgc tgacaacccc aggtattgat
    10861 gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg
    10921 cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt cctgctgact
    10981 ccggcagaag atatggccaa acttcagcaa cttgtgccct gctggtacgc ttcgaaaaac
    11041 tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa cagcatgaag
    11101 agcgctacgc cggttatacc ctgcgtcagt tgtgccagga aatgcatgat ttgtatgccc
    11161 gccacaacgt gaaacaactg caaaaagaga tgttccgcaa agcgcacttc ccacacgtca
    11221 gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag ctggttcgtc
    11281 tgccggatgc cgaaggtcgt atcgctgccg aaggggcgct tccttatcct ccgggtgtgc
    11341 tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg
    11401 aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag
    11461 aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca
    11521 ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca
    11581 gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa
    11641 gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg taggctcaat
    11701 ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gtggtatggg
    11761 cggttatgcc gagtatgctt tcggtaaatc cggtaacttt attgcgaact atacctacgg
    11821 cgtctcactg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga
    11881 actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg
    11941 gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac
    12001 cgtgtggggg ggcattattc cggtggtagg cctgtgcatt atcggctggt tctggtttag
    12061 cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcagtagg
    12121 ttcttccatc gctatgacgc tgtgggcttt tcttggtctg gagtctgcgt gtgcgaatac
    12181 tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg
    12241 tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc ctaatatgga
    12301 gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttta caccagaagt
    12361 gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca
    12421 gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt
    12481 ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca
    12541 gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt
    12601 taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat
    12661 tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt
    12721 tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag ccatgctgta
    12781 cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac cacgctttga
    12841 actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg cgaccgctct
    12901 ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc aaccgcttct
    12961 ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat cttatatgcg
    13021 tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac tttcagaccg
    13081 ccaatagaag caccgttgcc cggagcagca gtcaggcgcg cggtgatcgg gtcacctgcc
    13141 agggtgctgg cgctcaccat ttccggagac agcttagaca gcgccgcttt ttgtgcggaa
    13201 gtcgcagctg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc cagttcgttg
    13261 tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag cagacacata
    13321 atgatgccgt ctttgtcggt ggaccacggc gtgccgtcga aacgcaggaa ggaagcccct
    13381 gcgctctctt cgccgccaaa gccgaagctg ccgtcgaaca gaccatcaac aaaccatttg
    13441 aaacctaccg gtacttctac cagcttacgg cccaagtcat tgaccacacg gtcgatcatc
    13501 gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg acgatgctgg
    13561 aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc tgccggagtg
    13621 acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa cttatcacgc
    13681 agtgccagca gacccgccat cgcacactcg gaggagcagt ccatacggat cgcaccgtct
    13741 ttatcgaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat ggtcaggttg
    13801 aggttgtaat actcgccaat gcgcttccag tattcgatac cggaaccgcc caacggatca
    13861 acgcccagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc ggccagccct
    13921 tccacgaacg gctgcaccag atcctgctct ttcacatagc cggatgccat cgcttcgtcg
    13981 agggagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc cctgtcttcc
    14041 accactttgg tgacgttagt atcagccggg ccaccatttg gcggattgta cttgatacca
    14101 ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag cgggccacct
    14161 tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa gccattgttt
    14221 tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga aatgaacgca
    14281 ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat gccgtttttc
    14341 gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt aaagctgtgg
    14401 cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc cgcattccct
    14461 gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat caaatcactc
    14521 tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt tgtctgcaac
    14581 gttttagata gttccgcaaa ccttctcaat caattccgcc gggaattgca tcgactgcat
    14641 gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca cccaatacgg
    14701 cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg tttgttcatc
    14761 tgccgcaaag tacacgcgtg tacgaccgtg caacgattcc gttgcttcgg caaacgcctg
    14821 ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg ctcgcttttg
    14881 ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt ctttaatcgt
    14941 tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt tcgtcaccgg
    15001 agcagcaggc tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt cggatgcgct
    15061 ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat caacttcaat
    15121 cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccaa gagtatatga
    15181 ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg aataaacctt
    15241 atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac aacgcgaaaa
    15301 aaagaacttt gccatgaaat taaatatccg cgcgcaaact gcacaaaacc agcacaataa
    15361 ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg gcgtactggc
    15421 tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc acggtctttc
    15481 accgagagag ccggtaatga attacccggc gatggcgcag gatcttgttg ataccctgga
    15541 tgcactgcag atcgacaaag caacatttat cggtcactcc atgggcggta aagcggtaat
    15601 ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg atatcgcacc
    15661 ggtcgactat cacgtacgcc gtcatgatga gattttcgca gctatcaacg cggtcagtga
    15721 atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc ttaatgaaga
    15781 aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct ttaacgtgcc
    15841 ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg catgggatca
    15901 ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt accgtgatga
    15961 tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg gtcactgggt
    16021 ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca atgattaacg
    16081 caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg gcgtgactca
    16141 tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg attgtttccc
    16201 ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta gatctgttcg
    16261 cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat gaacagctgc
    16321 gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag cgtgtcgaac
    16381 tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggaatcg cgtaatatga
    16441 gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg cgtagtcagg
    16501 gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg tttgctgatt
    16561 ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt ccacccccat
    16621 ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt tttcggcagc
    16681 gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg taaagacgtt
    16741 gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta cgacattctg
    16801 ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga tgacttcttc
    16861 ccgactctcg aagagattga tttcaatggt aaactggttg cgctgtttgg ttgtggtgac
    16921 caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga catcattgaa
    16981 ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt cgaagcatca
    17041 aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga ccgtcagccg
    17101 gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga gttgcatctc
    17161 gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc cgtattaata
    17221 gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt acctgtacaa
    17281 tgtcccagtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg tttttattaa
    17341 caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata atgatacgca
    17401 ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta gtaacaggac
    17461 agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa agtaacgctt
    17521 cctcgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt cagtgcggaa
    17581 gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac ggtatatcgc
    17641 gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga aggcggtaaa
    17701 tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct cgactgcggc
    17761 aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat tgccgcaaaa
    17821 catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc cgaaggcgat
    17881 tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga gaaaagccaa
    17941 cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac agctctcact
    18001 tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt caccgcctga
    18061 tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg tacactttcc
    18121 tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac gctctggcag
    18181 gcttcaattt tttctggcga accttgcgcg gtgttgatac aggcgctgta tgcctctttc
    18241 agacggctgt cttcctgtgg tgcggttgat tgcgcacaag ctaccagccc cgatgccatc
    18301 atggcgatga gaatcagttt tttcattgaa tgcggcaggc acaaaacctg ccgcgtcggg
    18361 catcagaaga tggtgaatgg tgcgattacc ataaatttca cgtcacgctc atcctggaag
    18421 atgttaccgt aaccgccgcc ccagcttggg atgtcggagt ggttgtcgta ttcggtgaag
    18481 tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa tggtgtagac cgcatccagg
    18541 ctgtaggcag actcttcaat agtccggttt ttgtcgtagt acgcatccgg attgctctgc
    18601 caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag cgccgatggc gaagcctgga
    18661 agattccagt ttttcaggtc atacatcgca ctgaagaaga ccgctttttc gccgttagcg
    18721 ttgaagtcgg aacggttatc ccaccagata tccaggcggc cgttggagga agcgtaggtt
    18781 ggggtcatac gttgcaggaa gtatccctgc tgactgtcag ccttaatcca ggtgccttcg
    18841 aggcgcaaat ccactacgtc agccgcccgg taaccaaagg tcaacgcttg cagccaggcg
    18901 gtgccgtcat aaagatcgtt gacgctgcga tcgtcaactt tatcgcgggt accatagaac
    18961 tggtagctgg tggttaacgg gctaccggcg atatcaaatt tgtagctggc tttggcaaaa
    19021 tattgatcga tatacccttc cgcctgacca aatgccgctt ccagtacgaa gttatttttg
    19081 aagtcgtatt tcgcaccgat ggagtgcaga taatcaactt tggtggtttt atcgttctga
    19141 taaaactcat ccatttccag atgccacggc gctttgtatt cgttggtcca catgtaggag
    19201 aaactcaagg caccagcatc gccgtaatca aaattcgccc cggcttccgc cccctgataa
    19261 gtacccggca taaagctcca gtgcggcgct aacagcgttt gaccggttgg ctgaatgtaa
    19321 cctgccctcg cccaaaccgg accgtattta aatttggccg cagctttata caggcttata
    19381 ccgcttttgt cgccggacca gtcttcgtca taggctttat tactttttga aaacgcgatt
    19441 tcgttcgggt gggagctgtc gccgttttcc gccatttcaa tcgccgtaaa cgcagcaata
    19501 tcaaggccga acatatcagc agcataaccg gactgaaaat cgaggttggc attccaggtg
    19561 gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa catctttacg gtcgcgttca
    19621 cgctgccagt aatagatacc gccggttaag gttgaatcgt cgatgaatcc ttcggcgcga
    19681 gcctccggca ttgccataaa gcccgacatt gctgtaacac cggcaatagc cagcgccagc
    19741 gtactacgtt tgccactaaa cgtacgcatg ggttaatcct ctttgacgta taaattgctg
    19801 caccaaaggt gaacagcgaa aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc
    19861 gctgactaca ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca
    19921 gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt
    19981 tgcggttgaa aggcttgcag aagaataaaa acgtgaaatt ccagcaagat gaaagcggtt
    20041 acattttatt taattatgtt ttgtgatttt aaatttattt tatggataaa ttgcgaagcg
    20101 gaattaattc gcgaagaaaa gcagaaaaaa cgccgcagag cgcggcgttt gggattgttt
    20161 gctatttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc acgcaaattc
    20221 aatatattgc agagatcatg taggcctgat aagcgaagcg catcaggcaa tttagcgttt
    20281 gaaatttatt gtctatttat tgccggatgc gacgtaaatg cctcacccgg cttggtagaa
    20341 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20401 ccggtttttc ggccgtggaa tggcggctgt cgaggcagaa gtaaccttca cgctcaaact
    20461 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20521 ccagcgattc cgggttaatc accgacagga aatcatccgc agcacctggg ttaggcacgc
    20581 tgaacagacg atcatacaaa cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20641 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20701 aagtacagaa gatggtggtg atattacctt cggcatcttt ctccacgcgt tcagccttaa
    20761 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20821 tagcttcttc gcggaaatcg gcgcgatcga tccaaatttc accgctaaac ggcacctgac
    20881 ggctccccat ttccggtttg ttcggatggt tcggcatggt gaccatttcg ccttcgccct
    20941 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggcgcatttt
    21001 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    21061 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc cgcagtgtaa ccacgacgac
    21121 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    21181 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21241 cgtactggcg cggatgaaca ggaatggtga tgttatccag tacccagtcg tacagacgac
    21301 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc gcatcgctga
    21361 tgcaatgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21421 cagcaaattt aatgcggtac agcaccggat cgcgcattac gataaatggc gacgccatgt
    21481 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21541 acagcgccaa gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggctgcg
    21601 tcagggtacc gcggtattcg cggatctgtt ccggtgtcag ttcatcaacg tacgccaggc
    21661 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21721 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gaatcgacat
    21781 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21841 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    21901 cattcggctc cggcgggaaa cgggtgtgta ctgtgatgtg cttaccactg gccagatctt
    21961 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    22021 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    22081 tgttagtttt ttaaaattga taatctaaca aggattatgg ggatgattga tgaaaaaaaa
    22141 agcggcagag aagatctcca ccgcttgtca ttgttggatg cgacgctgcc gtgtcgcatc
    22201 aggcataaag cagattactt tttgatttca tacagcggtg tttgacccgc cacaacatgg
    22261 ccctgagctt taatgagcaa gccactgaaa tcgtcgatat tgctgcaaac caccgggcta
    22321 atcatcgagc gggcgttagc gttcaggtaa tccagatcca tttccagaat cggttgccct
    22381 gcgcttacct gcgcaccctc ttccaccaga cgtttaaagc ctttaccttc cagcgctacg
    22441 gtgtcgatac ccatatggac gacgatctcc gcgccttttt cggtttccag gcagaacgcg
    22501 tggttggtgt tgaagatttt cacgattgtc cctgcggctg gtgatacgac gattttatct
    22561 gtcggtttca ccgccacacc gtcacccacc gctttgctgg cgaatgcttc gtcaggaacc
    22621 tgatccagtg ccacgacatc accggtaatc ggcgatacca gctccgcgat agataccgcg
    22681 tttggtacag cctgcggttt tgctacaggc gcggcagttg ccggagttgc ttcagctgac
    22741 gcagcggcta ccggaccacg ggcaacgact ttcttcatcg catcgccgat ggattctgct
    22801 ttcgcgccaa caatcacctg aatagtctgt ttgttcagtt tcactacacc agaagcaccc
    22861 agacgtttac acatcgtatc gttgacgcgg gcagagtcag ccacggtcag gcgcagacgg
    22921 gtaatacagg catcaatcgc tttcaggttg tcagtaccgc caaccgcagc aatatagttg
    22981 gttgccagtt gagtcagacc ttcttcagta ttgctgttgg cttcttcagt aacgatctcg
    23041 tcttctttat cttcacgacc cggcgttttc aggttgaaca tgcggataac caaactgaac
    23101 accacgaagt agatagcgaa gaagataacg cccatcacca gcagcatcca gacgttctgg
    23161 ctggcggcag gcaggttata catcaacgcg tagtcgatag cacccgcaga gaaagagaag
    23221 ccggcatgga tacccagcag cgttgccaca aacaggctga taccggtcag cagtgcgtgc
    23281 aggaggtaca gcagcggcgc aaggaacatg aacaggaatt ccagcggctc agtcacaccg
    23341 gtcaggaacg cagtaacagc aacagaaagc agcatcccgc caaccatcgg acgacgctct
    23401 ttcggtgctg cgaagtacat cgccagcgcc gcacccggca gaccgaacat catgatcggg
    23461 aagaagccgg acatgaacat ccccgcggtg ccgtcaccag cgtagaaacg gttgatgtcg
    23521 ccgtggaaaa ccgtacccgc cgcgttggtg aattcaccaa tctggaacca ggcgatggtg
    23581 ttcagcacct gatgcagacc ggttgggatc agcagacggt tgatgaaacc aaagataccg
    23641 gaacccagcg cgcccgcaga aacgatccac tcgccgcctg catggatagc gtgctgtacc
    23701 ggcggccaga cgtaaccaaa aatggccgcc agcaccagac agaagaagcc ggtggcgatc
    23761 ggcacaaagc gtttgccgcc gaagaagctc aggaagtccg gcagtttaat atcggaccaa
    23821 cggttatagg ctgcgccacc aaccagaccg gtaatgatac ccgccagtac acccatgtta
    23881 atttctgggt tgatggtcac catcgctttg gttaacacaa agtaacctac cgcacccgcc
    23941 agcgccgccg cacctgcgct gtctttcgac cagctggatg ccacaccgat ggcgaagatt
    24001 aatgcgaggt tatcaaaaat tgcaccgccc gcctgggcaa taaacgcaac gttaagtaaa
    24061 tctggctgac cgaatcgcag caacagcgcc gccaccggca gcaccgcgat agggagctgt
    24121 aacgccctac cgagtcgctg gaaaaaacct aaaatattca tcttattccc cctacgagaa
    24181 ccctatttgg ctcgtttaaa gccgtatttt tattttgctg caaattttac tgccgatgtt
    24241 ctgtaatcag attgttagat catctgctac agagtgtgtg aaaatttaat tcgcatcgca
    24301 aattaaacgc gtgtcttttg tgagttttgt caccaaatat cgttatcatc actccctttt
    24361 actggctaaa ccagaaaact tattttatca ttcaaaaaat caagtcggat tgacgcctgt
    24421 ctgcgcaaat ccaggttacg cttaaagatg tctaatccgc caacggctta cattttactt
    24481 attgaggtga ataatgagac tgatccccct gactaccgct gaacaggtcg gcaaatgggc
    24541 tgctcgccat atcgtcaatc gtatcaatgc gttcaaacca actgccgatc gtccgtttgt
    24601 actgggcctg ccgactggcg gcacgccgat gaccacctat aaagcgttag tcgaaatgca
    24661 taaagcaggc caggtcagct ttaagcacgt tgtcaccttc aacatggacg aatatgtcgg
    24721 tctgccgaaa gagcatccgg aaagctacta cagctttatg caccgtaatt tcttcgatca
    24781 cgttgatatt ccagcagaaa acatcaacct tctcaacggc aatgccccgg atatcgatgc
    24841 cgagtgccgc cagtatgaag aaaaaatccg ttcttacgga aaaattcatc tgtttatggg
    24901 cggtgtaggt aacgacggtc atattgcatt taacgaaccg gcgtcttctc tggcttctcg
    24961 tactcgtatc aaaaccctga ctcatgacac tcgcgtcgcg aactctcgtt tctttgataa
    25021 cgatgtcaat caggtgccaa aatatgccct gactgtcggt gttggtacac tgctggatgc
    25081 cgaagaagtg atgattatgg tgctgggtag ccagaaagca ctggcgctgc aggccgccgt
    25141 tgaaggttgc gtgaaccata tgtggaccat cagctgtctg caactgcatc cgaaagcgat
    25201 catggtgtgc gatgaacctt ccaccatgga gctgaaagtt aagactttaa gatatttcaa
    25261 tgaattagaa gcagaaaata tcaaaggtct gtaattgtta tccctgccct ggttccttgc
    25321 tcagggcaat attttttaaa atcgggggtc agaatgtatg cattaaccca gggccggatc
    25381 tttaccggcc acgaatttct tgatgaccac gcggttgtta tcgctgatgg cctgattaaa
    25441 agcgtctgtc cggtagcgga actgccgcca gagatcgaac aacgttcact gaacggggcc
    25501 attctctccc ccggttttat cgatgtgcag ttaaacggct gcggcggcgt acagtttaac
    25561 gacaccgctg aagcggtcag cgtggaaacg ctggaaatca tgcagaaagc caatgagaaa
    25621 tcaggctgta ctaactatct gccgacgctt atcaccacca gcgatgagct gatgaaacag
    25681 ggcgtgcgcg ttatgcgtga gtacctggca aaacatccga atcaggcgtt aggtctgcat
    25741 ctggaaggtc cgtggctgaa tctggtaaaa aaaggcaccc ataatccgaa ttttgtgcgt
    25801 aagcctgatg ccgcgctggt cgatttcctg tgtgaaaacg ccgacgtcat taccaaagtg
    25861 accctggcac cggaaatggt tcctgcggaa gtcatcagca aactggcaaa tgccgggatt
    25921 gtggtttctg ccggtcactc caacgcgacg ttgaaagaag caaaagccgg tttccgcgcg
    25981 gggattacct ttgccaccca tctgtacaac gcgatgccgt atattaccgg tcgtgaaccg
    26041 ggcctggcgg gcgcgatcct cgacgaagct gacatttatt gcggtattat cgctgatggc
    26101 ctgcatgttg attacgccaa cattcgtaac gctaaacgcc ttaaaggcga caaactgtgt
    26161 ctggttaccg acgccaccgc gccagcaggt gccaacattg aacagttcat ttttgcgggt
    26221 aaaacaatat actaccgtaa cggactttgt gtggatgaga acggtacgtt aagcggttca
    26281 tccttaacca tgattgaagg cgtgcgtaat ctggtcgaac attgcggtat cgcactggat
    26341 gaagtgctgc gtatggcgac gctctatccg gcgcgtgcga ttgacgttga aaaacgtctc
    26401 ggcacgctcg ccgcaggtaa agtagccaac ctgaccgcat tcacacctga ttttaaaatc
    26461 accaagacca tcgttaacgg taacgaggtc gtaactcaat aagagaaagt atgacaccag
    26521 gcggacaagc tcagataggt aatgttgatc tcgtaaaaca gcttaacagc gcggcggttt
    26581 atcgcctgat tgaccagtac gggccaatct cgcggattca gattgccgag caaagccagc
    26641 ttgcccccgc cagcgtaacc aaaattacgc gtcagcttat cgaacgcggg ctgatcaaag
    26701 aagttgatca gcaggcctcc accgggggcc gccgcgctat ctccatcgtc accgaaaccc
    26761 gcaatttcca cgcaatcggc gtacggcttg gtcgtcatga cgccaccatc actctgtttg
    26821 atctcagcag caaagtgctg gcagaagaac attacccgct gccggaacgt acccagcaga
    26881 cgctggaaca tgccctgctg aatgccattg ctcagtttat tgatagctac cagcgcaagc
    26941 tgcgcgagct aatcgccatt tcggtgatcc tgccagggct tgttgacccg gacagcggca
    27001 aaattcatta catgccgcat attcaggtag aaaactgggg gctggtagaa gcactggaag
    27061 aacgttttaa agtgacctgt ttcgttggtc acgatatccg tagtctggcg ctggcggagc
    27121 actacttcgg tgcaagtcag gattgcgaag actccattct ggtgcgtgtc catcgcggaa
    27181 ccggggccgg gattatctct aacgggcgca tttttattgg ccgcaacggt aacgtcggtg
    27241 aaattggcca tattcaggtc gaaccgctgg gtgaacgctg ccactgcggc aactttggct
    27301 gcctggaaac tatcgccgcc aacgctgcca ttgaacaacg ggtgttgaat ctgttaaagc
    27361 agggctacca gagccgtgtg ccactggacg actgcaccat caaaactatc tgcaaagccg
    27421 cgaacaaagg cgatagcctg gcctcggaag tgattgagta tgtcggtcgt catctgggta
    27481 aaaccatcgc cattgctatc aacttattta atccgcaaaa aattgttatt gctggtgaaa
    27541 tcaccgaagc cgataaagtg ctgctccctg ctattgaaag ctgcattaat acccaagcgc
    27601 tgaaggcgtt tcgtactaat ctgccggtgg tacgttctga gctggatcac cgctcggcaa
    27661 tcggcgcttt tgcgctggta aaacgcgcca tgctcaacgg tattttgctc cagcatttgc
    27721 tggaaaatta atgtgctttt atagtggcgc ttattgttgt caatattctg ggtagtccat
    27781 gaccattaaa aatgtaattt gcgatatcga cggcgtgctg atgcacgata acgtcgccgt
    27841 accgggtgca gcggaatttt tgcacgggat tatggataaa ggcctgccgc tggtgttgct
    27901 gaccaactat ccttcgcaga ctgggcaaga tctggcgaac cgctttgcca ctgccggtgt
    27961 cgatgtgccg gatagcgtgt tttatacctc tgcgatggcg accgccgatt tcctgcgtcg
    28021 ccaggaaggc aagaaagcgt atgtggtggg cgaaggcgca ctgattcatg aactgtacaa
    28081 agccggtttc actattaccg atgtgaaccc tgatttcgtg attgttggcg aaacacgttc
    28141 ctacaactgg gacatgatgc ataaagcagc ctatttcgtc gctaacggtg cacgctttat
    28201 cgccaccaac ccggacaccc acgggcgcgg tttttatccc gcttgtggcg cgttgtgtgc
    28261 agggattgag aaaatctccg ggcgcaaacc gttctatgtt ggtaagccca gcccgtggat
    28321 catccgcgcg gcattaaaca aaatgcaggc gcattcggaa gaaacagtga ttgtcggcga
    28381 taacctgcgt accgatattc tggcgggctt ccaggccggt ctggagacga ttctggtgct
    28441 ttctggtgtt tcatcgctcg acgatatcga cagtatgccg ttccgcccaa gctggattta
    28501 cccgtcagtc gctgaaatcg acgttatctg aataccaccc acgtctgcgg acgtggttgg
    28561 ctctgatgac aaacgccaaa ctgcctgatg cgctacgctt atcaggccta cgcagctcct
    28621 gcaatatatt gaattttcat gcttttgtag gccggataag gcgttcacgc cgcatccggc
    28681 atgaacaaat cgcactttat cagcaatttg gcccccgtaa aatataacca ttcaataaaa
    28741 cccgctaaaa attatcgatt catcaataaa tacatcctca caccagaccc tttttcacca
    28801 atcgacaatc accattacgt tttttatttt tgcactgcca aatagcttgc gcacagtgct
    28861 gctttgcggc atttttttaa acaagcaaac acaacaagca acaaatacca ggttaacgga
    28921 gaaggttatg tgttcaattt ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg
    28981 taagaaagca ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact ggtccggtat
    29041 ttatgccagc gataacgcca ttctcgctca cgaacgtctg tcaattgttg acgttaacgc
    29101 gggggcgcaa cctctctaca accaacaaaa aaaccacgta ctggcggtaa acggtgaaat
    29161 ctacaaccac caggcattgc gcgctgaata tggcgatcgt tatcagttcc agacagggtc
    29221 tgactgtgaa gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt
    29281 gcagggcatg tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc tgattggtcg
    29341 cgaccatctg gggatcatcc cactgtatat ggggtatgac gaacacggtc agctgtatgt
    29401 ggcctcagaa atgaaatcgc tggtgccagt ttgccgcacg attaaagagt tcccggcggg
    29461 gagctatttg tggagtcagg acggcgaaat ccgttcttat tatcatcgcg actggttcga
    29521 ctttgatgca gtgaaagata acgtgaccga caaaaacgag ctgcgtcagg cactggaaga
    29581 ttccgttaaa agccatctga tgtctgatgt gccttacggt gtgctacttt ctggtggtct
    29641 ggattcctca attatttccg ctatcaccaa gaaatacgca gcccgtcgcg tggaagatca
    29701 ggaacgctct gaagcctggt ggccgcagtt acactccttt gctgtaggtc tgccgggttc
    29761 accggatctt aaagcagccc aggaagtggc aaaccatctg ggcacggtgc atcacgaaat
    29821 tcacttcact gtacaggaag gtctggatgc catccgcgac gtgatttacc acatcgaaac
    29881 ttatgatgtg accactattc gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa
    29941 ggcgatgggc attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt ttggcggtta
    30001 tctttacttc cataaagcgc ccaacgctaa agaactgcat gaagagacgg tgcgtaaact
    30061 gctggccttg catatgtatg actgcgcgcg cgccaacaaa gcgatgtcag cctggggcgt
    30121 ggaagcacgc gttccgttcc tcgacaaaaa attccttgat gtggcgatgc gcattaaccc
    30181 gcaggataaa atgtgcggta acggcaaaat ggaaaaacac atcctgcgtg aatgttttga
    30241 gtcatacctg cccgcaagcg tggcctggcg gcagaaagag cagttctccg atggcgtcgg
    30301 ttacagttgg atcgacaccc tgaaagaagt ggcggcgcag caggtttctg atcagcaact
    30361 ggaaactgcc cgcttccgct tcccgtacaa cacgccgacc tcaaaagaag cgtatctgta
    30421 ccgcgagatc tttgaagaac tgttcccact tccgagtgcc gctgagtgcg tgccgggcgg
    30481 tccttccgtc gcgtgttctt cagctaaagc gatcgaatgg gatgaagcgt tcaaaaaaat
    30541 ggacgatccg tctggtcgtg cggttggcgt tcaccagtcg gcgtataagt aagaaatgcg
    30601 gtactgcccc ggagaaatcc ggggctgttt tagttattgc gctgcttgtt gattctgata
    30661 tgcagtaagg aaatgttccg ggtttgccac atcactgttt gacaactcat cactgtaggc
    30721 cagtatttca tagattaatt tcccaccact attgttgaat tccgaatttc atcctgtacg
    30781 acaaaagaat ggctgtaggg tggggaaaaa cacaaagtaa acaataattg acgaatatag
    30841 cgccacgctg ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga
    30901 ggttgacgct gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg
    30961 aaaaaaagat ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca
    31021 caggttcgaa tcccgtcgta gccaccatct tttttgcggg agtggcgaaa ttggtagacg
    31081 caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca
    31141 ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat
    31201 accggcattc cctggttcga atccaggtac cccagccatc ttcttcgagt aagcggttca
    31261 ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    31321 ctggttcgaa tccaggtacc ccagccatcg aagaaataat ctggctacgt agctcagttg
    31381 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccaa
    31441 attctgaatg tgtcgaatac gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca
    31501 agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc
    31561 caatttattc aagacgctta catcgtaagt gcacccagtt ggggtatcgc caagcggtaa
    31621 ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa
    31681 aaaagctcgc ttcggcgagc tttttgcttt tctgcgttca ttcaatgtcg aatgcgatgt
    31741 tgacacgtct tatccttcaa tgtcggatgc gacgctgccg cgtcttatcc gacctacggc
    31801 tggcacgcat ccggcaatgt tgtaaggcta caaccctaac gcatatttca gcgcctgacg
    31861 tttcaacacg ccagcacgct ctgccgccat taacccgaga ttacgcacaa aacgcagcgg
    31921 cggcagatta ttactgaatc cggcataaaa cagatccata ccactttgca taatgaagtt
    31981 atccgccatg cgccgcatct ggtaacgctt gaggacagga taactggccc acgcttcacc
    32041 gtagctgcgg gcattcacca gaacatcaat cagggcatcg acatcacgat aaccaagatt
    32101 caccccctgc cccgccagcg gatggatggt atgcgcggca tcgcccacca gcgcaagccc
    32161 cggctgcaca tactgcaacg catgtcggcg cgtcagcgga aacgcaccag cggcaagcgg
    32221 tgtaacgtaa cccagacgcg acgggaaatg cttcgcgatt tccgcctgga gttgcgccat
    32281 attcatattc tgcaactggc gaatacgcgc cggagagtca taccacacca gcgatgccca
    32341 gttatcaaac aacggcagaa acgcacgcgg tccgtccggg gtaaattgct gccaggtgct
    32401 gtcgccggga tcgttctcac actgtacgct aatcaacatg cacgactgcg catactgcca
    32461 ggcatgaacg ccaattcccg ccatctgccg cacctgagaa tttgcgccgt cggcaccaat
    32521 caccagtttc gcgcggatcg tttcaccgcc cttcagttcc agctcctgaa gatcattatc
    32581 tcgatgtaat gcaatcagcg aggttggcac acgtaacgtt actttcggat gcgcttccag
    32641 cgcctgccac aacgcctgtt gcaggactgt gttttccacc atgtagccaa gcaacggcaa
    32701 ctttaattcg gctgcgtcaa ataccacatg tgccgtttcc cactcccagg tttccagtct
    32761 gcggtaagga tggcaacgca tagcctgtac tgcatcccag acccctaacc ctttaagcaa
    32821 tgataccgaa gccgcgctga tcgccgagat ccgcacgtcc ggttggctgt cagcgacaaa
    32881 cggtgcaggt tctgcgtgct cgatcaccgt taccgaaaat ccgtgctgtg ccagccccag
    32941 cgccagtgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg gttgatttgt
    33001 catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta cccccaaggg
    33061 ctgataacgc tcatactggt cacaacggca gcaaagcatt acactatgcg ccctgcattc
    33121 ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg
    33181 ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg ccacccacgg
    33241 ctatcaactg accgacgtgg cggaagaagc ggatgtgcta ctgctgaaca cctgctcaat
    33301 ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac tgttaaaaga
    33361 gaagaatcca gacctgatta tcggcgtcgg tggctgcgtg gcatcgcaag aaggcgagca
    33421 cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct
    33481 gccggagatg atcaactccg tgcgcggcga ccgcagcccg gttgtagata tcagcttccc
    33541 ggaaatcgag aagtttgacc gtctgccgga accgcgcgcc gaagggccga ccgcgtttgt
    33601 ctccatcatg gaaggctgca ataaatattg cacctactgc gtggtgcctt acacccgtgg
    33661 tgaagaggta agccgtccgt ctgacgatat tctgtttgag attgcccagc ttgcggctca
    33721 gggcgtgcgt gaagtcaacc tgctcggtca gaatgtgaat gcctggcgtg gtgagaacta
    33781 cgacggcacc accggatcgt ttgccgatct gttgcgtctg gttgctgcga tcgacgggat
    33841 cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata tcatcgaagt
    33901 gtaccgcgac acgcctgagc tggtgagctt cctgcatctg ccggtacaga gcggttccga
    33961 tcgcattctg aacctgatgg gacgtaccca tacggcgctg gagtacaaag cgatcatccg
    34021 taaactgcgt gcggcgcgtc cggatattca gatcagttct gacttcattg ttggcttccc
    34081 tggcgaaacc accgaagact tcgagaaaac gatgaagctg attgccgacg tcaatttcga
    34141 catgagctac agctttatct tctctgcacg tccaggaacg cctgccgccg atatggttga
    34201 tgatgttccg gaagaagaga agaagcagcg tctgtatatt ctgcaagaac gtatcaatca
    34261 gcaagcgatg gcatggagcc gtcggatgct cggcaccacc cagcgtattc tggtagaagg
    34321 gacttcgcgt aagagcatca tggagctttc cgggcgtacc gaaaataacc gcgtggtcaa
    34381 cttcgagggc acaccggata tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta
    34441 cccgaactct ctgcgcggta aagtggtgcg aactgaagat gaaatgggtc tgcgcatggc
    34501 agaaacaccg gagtcggtga ttgcccgcac ccgcaaagaa aacgaccttg gcgtgggtta
    34561 ttatcagccg taattctcag gcctgccgtt ccggtgggcc ttgttttttc tctctttatc
    34621 cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt ccttataggc
    34681 cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa catagacact
    34741 cgcgaaatca ccctggagcc agcagacaat gcgcgtctgt tgagcctgtg cggcccgttt
    34801 gatgacaaca tcaagcagct cgaacgccgt ctcggcatcg agatcaatcg ccgcgataac
    34861 cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat tctgcgtagc
    34921 ctgtatgtcg atactgcccc gatgcgcggt cagattcagg atatcgaacc ggaacagatc
    34981 caccttgcga ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt gccggagtac
    35041 ggcaaagcgg tcaatatcaa aactaaacgc ggcgtaatta agccgcgcac gccaaaccag
    35101 gcgcagtaca tcgccaatat tctcgaccat gacattacct tcggcgttgg cccggcgggt
    35161 acgggtaaaa cctacctggc agtggctgcg gcagttgatg ccctggagcg tcaggaaatt
    35221 cgccgtattc tgctgactcg tccggcggtc gaagccggtg agaaactggg cttcctgcct
    35281 ggcgatttaa gccagaaagt agacccgtat ctgcgcccac tgtacgacgc gctgtttgaa
    35341 atgctgggct ttgagaaagt cgagaaactg attgagcgca acgttattga agtcgcgccg
    35401 ctggcctata tgcgtggtcg tacgctgaac gacgcgttta tcattctcga tgagagccag
    35461 aacactacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg
    35521 gttatcaccg gcgacgtcac acagatcgac ctgccgcgta atactaaatc aggcttacgt
    35581 catgccattg aagtgctggc cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa
    35641 gacgtggttc gtcacccggt ggtggcgcgt atcgttaacg cttatgaagc ctgggaagaa
    35701 gcagaacaaa aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga acaggaacaa
    35761 aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc gggttaccgg
    35821 aagagagcca gtttcaaaca tggctgaatg cggtgatccc gcagtttcag gaagagtcgg
    35881 aagtgactat tcgcgtggtc gataccgccg aaagccacag tctgaatctg acctatcgcg
    35941 gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtgccgcct ggcatggaga
    36001 tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc
    36061 aaggcaaacc actggaggcg cattgggcgc atatggtggt gcacggcagt ctgcatttgt
    36121 taggttacga tcacattgaa gatgacgaag cagaagaaat ggaagccctc gaaacagaga
    36181 ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa tttgccaggc
    36241 atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga ttaactaaca
    36301 tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca cgataagcaa
    36361 caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac cgaaaaaccg
    36421 tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg acgaagatac
    36481 gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg acatcatgat
    36541 cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat gccttgatgt
    36601 catcatcgag tccgcccact cacgtttccc ggtgattagc gaagacaaag atcacattga
    36661 agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg aagccttcag
    36721 catggacaaa gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg tagaccggat
    36781 gctgaaagag tttcgctctc agcgttacca catggcgatc gttattgacg aattcggtgg
    36841 ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg agattgaaga
    36901 cgagtatgac gaagaagatg atatcgactt ccgtcagctg agtcgtcata cctggaccgt
    36961 gcgcgcactg gcttccattg aagacttcaa cgaagcgttc ggcacccact ttagcgatga
    37021 agaagtcgac actatcggtg gtctggtgat gcaggcgttt gggcatcttc cggcgcgtgg
    37081 cgaaactatc gacatcgacg gttaccagtt caaagtggcg atggccgata gtcggcgtat
    37141 tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccaaagctgg atgaataaaa
    37201 ccgaaactgg atagataact acatggcttt tgcctcatta attgaacgcc agcgtattcg
    37261 cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc cttacgacgt
    37321 ctggcctgcg gcgattattt cgctgatggg gcttcaggcg ttgaccttta accgtcgccc
    37381 actccagtct gccgctattg gcttttgctg gggatttggc ctctttggca gcggtattaa
    37441 ctgggtctat gtcagcatcg cgacctttgg cgggatgcct ggcccggtta acatcttcct
    37501 ggtggtgctg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc
    37561 gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccacccctg ctctctggca
    37621 agtgaccgag tttctgcgcg gttgggtgct gactggcttc ccgtggttac agttcggcta
    37681 tagccagatt gatggcccgt taaaagggct ggcaccgcta atgggcgtgg aagccattaa
    37741 cttcctgctg atgatggtta gtggcctgct ggcactggcg ttggtcaaac gcaactggcg
    37801 tccgctggtg gtggccgtcg tgctgtttgc cctacccttc ccgctgcgtt acatccaatg
    37861 gtttaccccg caaccggaga aaaccattca ggtttcgatg gttcagggcg atattccgca
    37921 atcgctgaaa tgggacgaag gccagcttct taatacgcta aagatttact acaacgcaac
    37981 ggcaccgctg atgggcaaat catcgttgat tatctggccg gagtcggcga taaccgatct
    38041 ggaaattaat cagcaaccgt tcctcaaagc actggacggt gagttgcgtg ataaaggtag
    38101 ctcgctggta accgggattg tcgacgcgcg tctcaataag cagaaccgct acgataccta
    38161 caacaccatc atcacgctgg gtaaaggtgc accgtacagc tacgaatcag ccgatcgcta
    38221 taacaaaaac catctggtgc cgtttggcga gtttgtcccg ctggagtcga ttctgcgtcc
    38281 gttagcaccg ttctttgatc tgccgatgtc gtcgttcagc cgtgggccat atatccagcc
    38341 gccgctgtcg gcaaatggta ttgagcttac tgcggctatt tgctacgaga tcattctcgc
    38401 cgagcaagtg cgcgataact tccgcccgga taccgactac ctgctgacta tctccaacga
    38461 tgcgtggttt ggtaaatcta ttggtccatg gcaacacttc cagatggcgc gaatgcgtgc
    38521 gctggagctg gcgcgcccac tgttgcgcag caccaacaac ggcattacgg cggtgattgg
    38581 cccgcagggt gagattcagg cgatgatccc gcagttcgcc cgcgaggtgt taaccactaa
    38641 cgtgacgccg accaccggac tcacaccata cgcacgtacc ggcaactggc cgctgtgggt
    38701 gctgacggca ctgtttggtt ttgctgctgt gttgatgagt ctgcgtcagc gacgtaaata
    38761 atcccttctt atcgtgccgg aatacatccc atatttcggc acgctcaccg gatgcggcat
    38821 cagtgcatta tccggataac aatattctcc tatccaactt tggcacatct attgctttgt
    38881 tatacaaggc aaagcttgaa ccagcatcag tacaacccgg ttgcaccaca gcaatccacc
    38941 ggtagcacca aaagagtgca ttttaagaat tttgcttcag aatggtgcgg tgcacctcgc
    39001 aaaaacaaac aataaaacat caatatcttt acatttagcc aaactaaatg ttacctaaca
    39061 ggcacaacac tgcacaataa agttgcagac gataacaaca caaacactca caacgggtat
    39121 ccatgcgttc ttaacgcaga agataaaggt gttggatatg caattacgta aacctgccac
    39181 agcaatcctc gccctggcgc tttccgcagg actggcacag gcagatgacg ccgccccggc
    39241 agcaggcagt acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga
    39301 atcttcagta cctttctctt attacgacaa tcagcaaaaa gtggtgggtt actcgcagga
    39361 ttactccaac gccattgttg aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt
    39421 aaaactgatt ccgattacct cacaaaaccg tattccactg ctgcaaaacg gcactttcga
    39481 ttttgaatgt ggttctacca ccaacaacgt cgaacgccaa aaacaggcgg ctttctctga
    39541 cactattttc gtggtcggta cgcgcctgtt gaccaaaaag ggcggcgata tcaaagattt
    39601 tgccgacctg aaaggcaaag ccgtagtcgt cacttccggc actacctctg aagttttgct
    39661 caacaaactg aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca aagatcacgg
    39721 tgactctttc cgcaccctgg aaagcggtcg tgccgttgcc tttatgatgg atgacgctct
    39781 gctggccggt gaacgtgcga aagcgaagaa accagacaac tgggatatcg tcggcaagcc
    39841 gcagtctcag gaagcctacg gttgcatgtt gcgtaaagac gatccgcagt tcaaaaagct
    39901 gatggatgac actatcgctc aggtgcagac ctccggtgaa gcggaaaaat ggtttgataa
    39961 atggttcaaa aatccaattc cgccgaaaaa cctgaacatg aatttcgaac tgtcagacga
    40021 aatgaaagca ctgttcaaag aaccgaatga caaggcactg aactaattac aagaaccagg
    40081 ggcggaaatt ccagccctct cgattgttac gtagcacgga cagactatac gcctgatggt
    40141 cgttccccat cgggcctgaa aaccgcaata cgctgggtaa caatcttcga gggtagcagt
    40201 taacgctgct accctctttt ttctggagta gatttatgtc tatagactgg aactggggta
    40261 tttttttaca acaagccccg ttcggcaata ccacctatct tggttgggtc tggagtggtt
    40321 ttcaggtgac gatcgctttg tcgatctgcg cctggattat cgctttcctc gtcggttcat
    40381 ttttcggcat tttacgtacc gttccaaacc gttttctctc cggtctgggt acgttgtatg
    40441 tcgaactgtt ccgcaacgtg ccgttgattg tgcaattctt tacctggtat ctggtgatcc
    40501 cggagctgct gccggagaaa atcggcatgt ggtttaaggc cgagctggat cccaatattc
    40561 agtttttcct ttcatccatg ctctgcctgg ggctgtttac cgccgcccgt gtttgcgaac
    40621 aggtacgcgc cgctattcag tcgctaccgc gtgggcagaa gaatgccgcg ctggcgatgg
    40681 gcctgacgct accgcagact tatcgatacg tactgctgcc taatgcttat cgcgttatcg
    40741 tcccgccgat gacttccgag atgatgaacc tggtgaaaaa ctccgccatc gcctcgacca
    40801 tcggtctggt ggatatggcg gcgcaggcgg gtaaattgct cgactactcg gctcacgcct
    40861 gggagtcgtt taccgccatc acgcttgcgt atgtgttgat taacgctttt atcatgctgg
    40921 tgatgacgct ggttgaacgt aaagttcgcc tgcccggcaa catggggggc aaataatgta
    40981 cgagtttgac tggagttcca ttgttccttc cctgccatat ctgctcgacg ggctggtgat
    41041 caccctgaaa atcaccgtca cggcagtcgt gattggtatt ttgtggggta ccatgctggc
    41101 ggtgatgcgc ttgtccagct tcgcgcctgt ttcgtggttt gccaaagcct acgttaacgt
    41161 attccgctcg attcctttag tcatggtttt gctgtggttt tacttgattg tgccgggatt
    41221 tctgcaaaat gtgctgggat tatcgccaaa aaatgatatt cgtttaattt cggcgatggt
    41281 ggcgttttcg atgtttgaag cggcctacta ttcagagatt atccgtgccg gtattcaaag
    41341 tatttcccgt ggtcagtcga gcgccgcgct ggcgttgggg atgactcact ggcagtcgat
    41401 gaaactgatt attctgccgc aggctttccg cgcgatggtg ccgctgctgc tcactcaggg
    41461 catcgtactg ttccaggata cctcgctggt gtatgtgtta tctctggccg atttcttccg
    41521 taccgcctca accattggtg agcgtgatgg tacgcaggtc gagatgatcc tgtttgccgg
    41581 atttgtttat ttcgttatta gccttagcgc gtcgttgttg gtcagctact tgaaaagaag
    41641 gacagcataa tgattaccct gaaaaatgtt tcaaaatggt atggtcactt tcaggtgctg
    41701 accgactgct caaccgaagt gaaaaaaggc gaagtggtgg tggtttgcgg cccgtctggt
    41761 tccggcaaat caacgctgat taaaaccgtc aacggcctcg aaccagtgca gcaaggtgaa
    41821 atcaccgtcg atggtatcgt ggttaacgac aagaaaaccg atctggcaaa gctgcgctcc
    41881 cgcgtcggga tggtattcca gcatttcgag ctgttccctc atctgtcgat tatcgaaaac
    41941 ctgaccctgg cgcaggtgaa agtgcttaaa cgcgataaag cgcccgctcg tgaaaaagca
    42001 ttgaaattgc tggagcgcgt gggactttcc gcacatgcca ataaatttcc ggcgcagctt
    42061 tccggcggtc agcagcagcg cgtggcaatc gctcgcgcat tgtgtatggc tcctattgcg
    42121 atgctgttcg acgaaccgac atcggcgctg gatccggaga tgatcaacga agtgctggac
    42181 gtgatggtgg aactggcgaa cgaaggaatg accatgatgg tggtgaccca cgaaatgggc
    42241 tttgcccgca aagtggcgaa tcgggtgatc tttatggacg agggtaaaat tgtcgaagac
    42301 tcgccgaaag acgctttctt cgatgatccg aaatcggacc gcgcaaaaga cttcctcgcg
    42361 aaaatcctgc attaatcact ctggcgcaca tacccaattg cgcgccatct cacgctttat
    42421 cgtttcgccc ctcgtatctg ttacatcctg gcgttaccct tgtcgcaaag aagcacacaa
    42481 caaggagcaa caatggcact gccaattctg ttagattgcg acccaggtca tgatgacgct
    42541 atcgcaatag ttctcgcact cgcctcacca gagcttgatg tcaaagcaat tacgtcttcc
    42601 gccggaaacc agacaccaga aaaaacctta cgcaatgttc tgcgtatgct gaccttgctt
    42661 aatcgcaccg atattccggt ggcaggcggc gcggtaaaac cgttaatgcg tgagttgatt
    42721 atcgcggaca atgtgcacgg cgaaagcggt ctcgacggcc cggcattacc ggaaccgacg
    42781 ttcgcaccgc aaaactgtac ggcggtggag ctgatggcga aaacgctgcg cgaaagtgcg
    42841 gaacctgtca cgattgtttc taccggaccg caaactaacg ttgccttgct gctcaatagc
    42901 catccggaac tgcatagcaa aattgcccgt atcgtgatta tgggcggcgc aatggggctt
    42961 ggtaactgga cgcctgcggc tgaatttaat atttacgttg acccggaagc ggcagaaatt
    43021 gtcttccagt cagggatccc ggtggtgatg gccggtctgg atgttactca taaagcgcaa
    43081 atccacgttg aagacaccga gcgtttccgc gcgattggca accctgtttc aaccattgtt
    43141 gccgaactgc tggatttctt cctcgaatat cataaagacg aaaaatgggg ctttgtcggc
    43201 gcaccgctgc atgacccatg caccatcgcc tggctgttga aacccgagtt gtttaccact
    43261 gttgagcgct gggttggcgt ggaaacacag gggaaatata cccagggtat gacggttgtt
    43321 gattattatt atctgactgg caataaaccg aatgccactg taattgtcga tgttgatcgt
    43381 cagggctttg ttgatttact ggctgatcgt ctgaaatttt acgcttaaca ctcgtcattg
    43441 attaagatca ccacgcgtta cctgggtggt cttaatctat ttatctcttt ttctttcaag
    43501 atataaatgc aatggataat gcagaactcg ccataggtat cgatctcggc actaccaata
    43561 gtttaattgc cgtctggaaa gacggtgccg cgcaattaat tccaaataag ttcggtgaat
    43621 atttaacacc atccataatt agcatggatg aaaataatca tattttagtc ggaaaaccgg
    43681 ctgtatcacg gcgtacttcg catccggata aaacggcagc gttatttaaa cgtgcaatgg
    43741 gcagctggcg gttaggcagc gacacattta acgcgccaga actgtcctct ttggtattac
    43801 gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc gattaaagat gtggtgatct
    43861 ccgttccggc ttatttcagt gatgaacaac gcaagcatac ccgtttagca gcggacttag
    43921 ccgggttaaa tgcggtacgc ttaattaatg aacccacagc agctgcgatg gcgtatggcc
    43981 tgcataccca acaaaatacc cgttcgctgg tgtttgatct cggtggcggc acgtttgacg
    44041 ttacggtgct tgagtacgcc acgccggtga ttgaagttca cgcctccgct ggcgacaact
    44101 tcctcggtgg cgaagatttt acccatatgt tggtcgatga cgttttaaag cgcgcggatg
    44161 tcacaaggac cacgcttaac gagagtgaac tggcagcctt gtacgcctgt gtggaagcgg
    44221 caaaatgtag caatcaatcg ccaatgcaca ttcgctggta gtaccaggat gaaacgcggg
    44281 aatgcgaatt ttacgagaac gaactggaag atttgtggtt gccgctgctc aatcgcttgc
    44341 gagtgccgat tgaacaggcg ttgcgcgatg cgcgtctgaa gccgagtcaa atcgacagtc
    44401 tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt
    44461 ttggcaaatt accgtatcaa agttacgatc cgagcaccat tgtcgcgctg ggcgcagcaa
    44521 tccaggccgc ctgccgctta cgcagtgaag atattgaaga agtgatcctc actgatattt
    44581 gcccttactc gttgggcgtt gaggttaacc gccagggcgt ttccggcatt ttctcgccga
    44641 ttattgaacg aaacaccact gtgcccgtgt cgcgtgtaga aacttattca accatgcacc
    44701 cggaacagga ttcaattacg gttaacgtct atcagggaga aaaccacaaa gttaaaaaca
    44761 acattctggt ggaatccttc gatgtgccgt tgaagaaaac cggggcttat cagtcgattg
    44821 atattcgctt tagttatgat atcaacgggt tgcttgaagt tgacgtgctt ctggaagacg
    44881 gcagcgttaa gtccagagtg attaaccaca gcccggtaac attgagcgcg cagcagattg
    44941 aagagagtcg gacgcggtta tccgcattga aaatttatcc gcgcgatatg ctcatcaatc
    45001 gcacctttaa agccaaactg gaagagttgt gggcgcgggc gctgggtgac gagcgagaag
    45061 agattggccg ggtgatcacc gattttgatg cggcgctgca gtcaaacgat atggcccgcg
    45121 tcgatgaggt caggcggcgg gcgagcgttt atttagccat tgagacctca taatcacggc
    45181 tgccggatgc ggcgtaaatg ctgcatccgg catcggcgca aaaaacacat cgtcaactaa
    45241 acaacatcgc gattttgccg agagcataac ctataaccag caccgttaaa ctgattttga
    45301 taatgctggt aatgctctgt ggtttttcac cctcatcatt tttttcttca tggttttcag
    45361 gtgttggctc atcgacatct tcatcctccg gctcgagttt tactttcgtc aacagcatcc
    45421 gggtgataat ggcctctaat ggatgagcat ctccgccttc cagccttgta ttaagacggg
    45481 ccagcatctc ttgtttcgga aacgcttcaa gtactacctg ttccgctacc cgatacaaat
    45541 ccagtaacgg tgaatcttct tctgtctcgc taccgactct cgatttccac cacgcccatg
    45601 ctttgcgttt gaagaataac gactcaataa acgattgacc taacggcgat aattttccgc
    45661 tatgtaaaat ttccgcccat aacagccgcg tccgggcgct ctcaccagga acatatttca
    45721 gcggttgacc ttgcgcatct accatgctcc gcgcttcgat aaccatcact aataacgcga
    45781 aataatccgg gcaatgacgc ttgctccact gcaacaacaa attatcagct tgtgtagatg
    45841 ggtaactttc ccagtacgcg ccgagatcag caagcaggct ttccccctcg ccgcagtaca
    45901 aacgttgtgc acactggtaa taccgggcat cttcgctttc tggttcttcc gcttgccact
    45961 gtttggctac aggaatgagt tccaccatac ctgtttgcac cgagctgtac cagcgcaaca
    46021 gtttgcgatg taactttgca tcatcaggaa tgaaccacgg tccttccatc gccaaaaacg
    46081 cagtgacata gtcaggatgg taacggcagc aacgttccag cgcgaagtaa tattcaacag
    46141 tctggttctg taacgcgacg ggatattcca gaaggctaag gaaatcgaaa caatcaccag
    46201 cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc cattccttcg ctgtcattaa
    46261 acgaatgcca gttcaaacgc tctgccaaca aagaagcaca atttaatgac agatatcgcg
    46321 cttctatagc gattgcacac agcggccagc gtaattgatc gacatcctcc atgttccagg
    46381 tatttaattg ctgaataaat ttctgccagg cggagggctg aaaacgatca ctttctgaat
    46441 ccagtaatgt cctgaatgca cgtagtattt catgttcagc ggtggcatct ttttcttcat
    46501 catctgcttc ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag gcctggcgta
    46561 atgctttaaa cccctgcgga tcggtttcgg gatgacacaa cggtaagcga gcaagataag
    46621 cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt
    46681 tcataggtcg ctcacatcct taagcgttac cgggcattca ggctggacag ttaatggctg
    46741 ccagggaagc gtggcataga aacggtcgct tcccgctgcc agctcttcga acatccgact
    46801 cccggcgcta tcgtatttgt acagggttag cgcctcgcgt agcgaagcgt tgtcttcgca
    46861 ctgagaagat tgccagtaga gacgccctag tgaataggca gaaaaatagt gcatccagct
    46921 gtgatagtaa tggtgagctc gtgcatagac gcgcgattgc agccataaac tctcttcttc
    46981 cgtcagccag ttgttgagca caccggcccg tgacagaaac cccattcgaa cataatccca
    47041 ggctttgatt cccccttctc cacagcacat agcggtttgg gcaacgaatt gggtataaat
    47101 caaccctctc tccgagccac cagcaattaa tgctttccac tcttcagggg aagagcgttg
    47161 ccatgtcaga tataagccag ctaaatcatt ggcatgacca tcatcagtca tctgataaat
    47221 agtctgaatt aaatcatcac gattatttat tccccaggat gatgagagat cgaattcttt
    47281 tatcccggta tacatttttg gcgagtcatg cgcgggtttg caactctgat taagtatttc
    47341 catgggcgca gaaagcgcaa aaagaagata ttgttcttcc ttattcattt tttaataatc
    47401 ccttaagtgc attaactgac tactttttta ttattgatga gtataaactt gacgctttca
    47461 aaataaaaag aaaatcgaag cattcacaca tgaataaaaa attaatgtat atattcgcaa
    47521 tttttatagt tgcagcaatt acctgtatta gccaacccaa gaaaacgatg ttgcgtgata
    47581 aagccatggt gaattatgcc tttgattatt taagatcacc aggcagtcta ccgttaacca
    47641 cggcagccac ggagctttcc gcgattcatg gtcactcaac atcgcaatat cgccttggcg
    47701 aattttatct tcatggtagc gacggtaaac cactggatta tacacaggcg agatactggt
    47761 atgagcaatc agcagaacag gaaaatccgc gcgcacaaag caaactgagg tggatctacc
    47821 tgaaaggcct gggagtcaaa cccgataccc gtaaagcaat tctctggtat aaggaagcag
    47881 ctgaacaagg gtatgctcat gctcaatata ctttgggttt gatctacaga aatggcactg
    47941 gtattaatgt taaccattat gaatctcaaa aatggttaaa actggccgcc aaacaacatt
    48001 acaaaaatgc ggaaagatta cttgccggac ttcccgcaca ttaacgtcga ctgtcacccg
    48061 aagagatgga gtattttccc tatcaaaccg gcaaagataa aaatgtagaa aataattttg
    48121 atgacgccca gacagcccag cccgccatca tcaacgggca ttggctgtgg ttgcggctcc
    48181 ggctcgacgg tttcctgacc ggtaaaattc gccgcctgaa ttaataatcg acgaataatt
    48241 aatgcttcca gtggacaacg atcttctgca tcaaggcggc tcacaatcgg cattaatgat
    48301 tcttctgtcg gttggctggc ctgcactaat tgtttggcaa ctaaatataa tggccataag
    48361 ggatgttctg tatcacgcgt gagtgagatc cgcggcttac gtccttcgtg caaaaccatc
    48421 tcgaccagtg aacgccccag cgcagagagt ttttcatcgc tgagtatttc gtataaatga
    48481 aaacgcgtac gggcgctgtc gccggggaca taaagtagcg gttttccctg atcgttaacc
    48541 agatcacgcg cttcaatcat catcaccact aatgggtaat aatagacccg atgctgacgg
    48601 caccattgca acattaaagc atccgcctgg gtggagggat actcgcgcca gtagtaacaa
    48661 agttctgcca gcaggctttc gccttctcca caatagacgc gttgtgcgta ttcataataa
    48721 cgcgcatctt cattgtcagg ttcagcttcc cgccacgacc gggcgtaatc caggagttcg
    48781 gggattccca ggcggcttgc gctataccag cggagtaaat ttctgtgcag ttttgcatca
    48841 tcggggataa tcactgcgcg atgttgcgct aaatacaatg tcagccattc tggatgcgat
    48901 gaccaaatcc gagcgaacat ttggtaaaaa tcgatggtgt cattttgcac ggcaactggc
    48961 agatgcagaa tggtctggaa gttaaaaaca ttccccttcg caatggcata taaaaaggat
    49021 tccagttctt cttcgtctat ttccccgacg ttttcctcct gcaaccaccg caatctttct
    49081 gctaataaca ccacgcactc gaaggataaa tgggcagtgt tcatggcgat tgtgcacagc
    49141 gaccagcgta attcatcaat ctcttccatc gagcaataat ttaattgctg aatgaatcgc
    49201 tgccaggcgg aggacagaaa acgttcgcta tcagaggcaa gtaacgcgcg aaaggcgagc
    49261 agaatttcat gttctgctac ctcatattct tctggttgcc aaacagattt agcaggcgac
    49321 tgcgcaatcc gtagcgcctc ctcatacgcc tgacgaagtt gtttaaaacc ctgcggatcg
    49381 gtttctggat gaaaggacgg taacagcgcc agataagcgc ggcggataat atcgacgtca
    49441 gtcgtttcct ctatatcgag gatcttccag caatttttca taagtcgctc atatccttaa
    49501 gcgtttccgg gcatgtgggg tagtcagcca gtggttgcca ggacaaggta gggtaatacg
    49561 catcatcact ggcaaataat tgataaaaca tatcattgcc ggaatcgtcg tattctttac
    49621 gcgctaagaa ttcgcgtaat aaaggcagat gatcatcttc gggagactgc caatattgcc
    49681 gaccaaaggt ataaccggca aagtattgcc gccagttact ataataacgt agagcccgta
    49741 gatgaatacg cgactggatc catagacttt cttcttcact caaccagtta ttaagtacgc
    49801 ccattcgact taaaaaaccc atccggacat aatcccatgc tttaattcct ccctcaccac
    49861 agcattcggc tgtgctggcg acaaattgcg cataagcctg accttgctca tttaattcag
    49921 caagatagtc acgccattca catggagaat aacgaaacca gcggtgataa aaccctgcca
    49981 accgagcggc atgaccgttc tcgatcattc ggtgaattat gcgcagcagg tcatcacggt
    50041 catcaattcc ccatgaggaa gaaaggtcaa aaaagcatgt acctaaatac attttggggg
    50101 atgaatgcga aggtaaacag cattcattgc gtatttccat cggagtggaa agtgcaaaca
    50161 atattttttg cgaccccatg tccatataat ttatccttaa acattcattt aatgaatgta
    50221 aactacattt cgaggctcag ttatttatac ttttctgacg caacataata cgcataaaat
    50281 aaggcttacg caaaaatgga atacataaaa atataatata ttttatctac aaccataagg
    50341 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
    50401 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
    50461 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgctg
    50521 agcaaggtca ttgtgaagca cagtattcct tagggcaaaa gtataccgag gataaaagcc
    50581 gtcataaaga taatgagcaa gccatcttct ggctgaaaaa agctgcccta caaggccata
    50641 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aactataaag
    50701 aagcggttac ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
    50761 ttggatggat gtacagaaat ggcaacggag tcgcacaaga ctatacgctg gcatttttct
    50821 ggtacaaaca agctgcatta caaggccata gttacgcaca agacaatctg gccgatcttt
    50881 ataaagacgg agaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
    50941 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
    51001 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
    51061 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
    51121 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
    51181 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
    51241 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
    51301 ttcgtgagac tgaagcttta ctgtagaaat aaaaacaagg gccggggaat ttcattctgc
    51361 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
    51421 aactggtcat aaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
    51481 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
    51541 accccatgat gattgaggtc ctggaaaatt tcgcgccatt caagctgcgt tttatcggtg
    51601 atgtctgcca gctcctgcca caagctttct ttaatcaccc gcataaagac gctgtcagat
    51661 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tctgacagct
    51721 cgcgtcagct catcgatctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
    51781 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gttcagtgac
    51841 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
    51901 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
    51961 taaattaatg tacgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
    52021 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
    52081 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
    52141 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
    52201 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
    52261 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgttaccag cgcatgctgg
    52321 gcaaaaacgt tctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
    52381 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
    52441 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
    52501 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
    52561 ataaaaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
    52621 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
    52681 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
    52741 atcactggcc agacaccgtt aaaaccatgc agcgtaactg gatcggtcgt tccgaaggcg
    52801 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
    52861 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
    52921 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
    52981 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
    53041 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
    53101 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
    53161 ctaaatacgg cctgaacatc aaaccggtta tcctggcggc tgacggctct gagccagatc
    53221 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgag ttcaacggtc
    53281 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
    53341 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgccagcgt tactggggcg
    53401 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccaaccccg gacgaccagc
    53461 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
    53521 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
    53581 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
    53641 aaggtatgct ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg
    53701 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
    53761 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
    53821 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
    53881 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
    53941 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
    54001 cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
    54061 ctccggctga tatgactctc gaatggcagg aatccggtgt ggaaggggct aaccgcttcc
    54121 tgaaacgtgt ctggaaactg gtgtacgagc acacagcaaa aggtgatgtt gcggcactga
    54181 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaactatcg
    54241 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
    54301 tggagctgat gaacaaactg gcgaaagcac cgaccgatgg cgagcaggat cgcgctctga
    54361 tgcaggaagc actgcaggcc gttgtccgta tgctcaaccc gttcaccccg cacatctgct
    54421 tcacgctgtg gcaggaactc aaaggcgaag gcgatatcga caacgcgccg tggccggttg
    54481 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
    54541 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
    54601 gccaggaaca tctggtagca aaatatcttg atggcgttac tgtacgtaaa gtgatttacg
    54661 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
    54721 ggcaacactg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
    54781 tgacaccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
    54841 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
    54901 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
    54961 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
    55021 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
    55081 ttcattcttc gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
    55141 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
    55201 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
    55261 tgcacgcgtc tccaccatgc tgggtaactg atgattcggt tgtacccgga acaactccgc
    55321 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
    55381 ttgcaggaaa gccaggacgc tgttcgccag gtagctgcgg cacaaggatt cgaagaacac
    55441 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
    55501 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
    55561 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctattg
    55621 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgctctt
    55681 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
    55741 gttgctgtgc gcgcaaaaca gctcaactta gaactggatg acgcggcgaa tcaggtgctc
    55801 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
    55861 ctctggccag acggcaaact gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
    55921 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcgttg
    55981 catattcttc agcaactgcg tctggaaggc agcgagccgg ttattttgtt gcgcacatta
    56041 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
    56101 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
    56161 aatcgcttaa gtcagccgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
    56221 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
    56281 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
    56341 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
    56401 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
    56461 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
    56521 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
    56581 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
    56641 tggtcaggat tcattgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
    56701 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
    56761 ccagcaatgg ctggaagatc atttgacaca taacccggaa gatcttcacc ttcagcctgc
    56821 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
    56881 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtat tgacttacat
    56941 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
    57001 ttaattcgtc atggcgaaac acaagcgaat gtcgatggtc tgtacagcgg tcatgcgccc
    57061 acacccctga ccgcacgcgg tattgagcaa gcgcaaaatc tgcatacact gctagatgat
    57121 gtttcctttg acctggtttt atgcagtgaa ctggaacggg cacagcatac cgcgcgactg
    57181 gttctcagtg accgccagct ccccgttcat atcatccctg aactcaacga aatgtttttt
    57241 ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag aagatgccga aaactatagc
    57301 gcgtggtgca atgactggca gcatgctatc cccacgaatg gtgaaggatt tcaggcattt
    57361 tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat atcagcacta tcagaatatt
    57421 ttaatcgtca gccatcaggg cgtactgagt ctgttaattg ctcgtttaat tggcatgcct
    57481 gccgagtcta tgtggcattt tcgcgttgac caggggtgtt ggagcgccat tgatatattc
    57541 gcgacgctac gcgtcctcaa tagccgtgcc atcggggtcg agaacgcatg acttttctgt
    57601 ttttttacgg gtaagccgca acgaccattg acagccccgg gcaggctgat attctccgca
    57661 gccagacttt ttccgccaga cacgactttg tagaaattgt tttacaaaaa tggcgatgca
    57721 atctgcggcg cggggtggga tgatagccca ctttcgaaag ccgattcggc gacaattgtc
    57781 ccgaaatcgc ctctggttca ggtatactga cagaccattt ttatctattt gattcaccca
    57841 gggggaaaac ttgcagggta aagcactcca ggattttgtt atcgacaaaa ttgatgacct
    57901 caaaggtcag gacatcatcg ccttagacgt tcaaggcaaa tccagcatca ccgactgcat
    57961 gatcatctgt acgggtacgt ccagccgtca tgttatgtcc attgctgacc acgttgtgca
    58021 ggagtctcgc gcagcgggcc tgttaccgct cggcgtagaa ggtgaaaaca gcgccgactg
    58081 gattgtcgtg gatttgggcg atgtgattgt ccatgtcatg caggaagaga gccgtcgcct
    58141 gtatgaactg gaaaaactct ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac
    58201 gaaaatgccg gactgggtac aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga
    58261 tatgcccttc gagctgattg aaattccggc cggcaaacgc ggcaagaatg cggacatcaa
    58321 gcgcatactc gacaaagagg gtgagcagat gttggcggcc gcaggcaaaa accgcattgt
    58381 caccctcgat attccaggca agccctggga tacgccgcag ttagccgctg agctggaacg
    58441 ctggaagctg gatggtcgcg acgtaagtct actgattggc gggcctgaag ggttgtcgcc
    58501 tgcctgtaaa gcggcggctg agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc
    58561 gctggttcgc gtgctggtcg cagagagtct gtaccgggcg tggagcatca ccaccaacca
    58621 tccttatcac cgtgagtgat aagggagctt tgagtagaaa acgcagcgga tgaaactaca
    58681 gaactctttt cgcgactata cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc
    58741 ctttttgggg attttgctgc tgaccggcgt gcttatcgcc aacctgtata atctgcaaat
    58801 tgttcgcttt accgactacc agacccgctc taatgaaaac cgcattaagc tggtgcctat
    58861 cgcgcccagc cgcggcatta tctacgaccg taacggtatc cctctggccc tcaaccgcac
    58921 tatctaccag atagaaatga tgccggagaa agtcgataac gtgcagcaaa cgctggacgc
    58981 tttgcgcagc gtggtagatc tgaccgatga cgatattgct gcattccgaa aagagcgcgc
    59041 acgttcacac cgtttcacct ctattccggt gaaaactaac ctaaccgaag tacaagtagc
    59101 tcgctttgcc gtcaatcagt accgttttcc gggtgtcgaa gttaaaggct ataaacgtcg
    59161 ttactatcct tacggttcgg cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga
    59221 taaagacgtc gaacgcctga ataatgacgg caaactggcc aactatgcgg caacgcatga
    59281 tattggtaag ctgggcattg agcgttacta tgaagacgtg ctgcacggtc agaccggtta
    59341 tgaagaggtt gaagttaaca accgtgggcg tgttatccgc cagttaaaag aagtaccacc
    59401 gcaagccgga cacgatattt acctgacgct ggatctcaaa ctccagcaat atattgaaac
    59461 gctgctggcg ggtagccgcg cagctgtggt tgttaccgaa ccgcgtacag gtggggtgct
    59521 ggcgctggtt tccacgccta gttatgaccc aaacctgttt gttgacggta tctccagcaa
    59581 agattattcc gccttgctga acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg
    59641 ggtttatcct cccgcgtcta cagttaaacc ctatgtggcg gtttcggcat taagcgccgg
    59701 ggtgatcacg cgcaatacga cgctgtttga cccaggctgg tggcaactac cagggtcgga
    59761 aaaacgttat cgtgactgga aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct
    59821 ggaagaatct gcggatacct tcttctatca ggtggcctac gatatgggga tcgatcgcct
    59881 ctccgaatgg atgggtaaat tcggttatgg tcattacacc ggtatcgacc tggcggaaga
    59941 acgttccggc aacatgccta cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta
    60001 tcagggtgac accattccgg ttggtatcgg ccagggttac tggacagcga ccccaatcca
    60061 gatgagtaag gcactgatga tcctgattaa tgacggtatc gtgaaggttc cgcatttgct
    60121 gatgagcacc gccgaagacg gcaaacaggt gccatgggta caaccgcatg aaccgcccgt
    60181 cggcgatatt cattccggtt actgggagct ggcgaaagac ggtatgtacg gtgttgctaa
    60241 ccgccctaat ggtacggcga ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa
    60301 atctggtacc gctcaggtct tcggtctgaa agcgaacgaa acctataatg cgcacaaaat
    60361 tgccgagcgt ttacgtgacc acaaactgat gaccgccttt gcgccataca acaatccgca
    60421 agtggctgtc gccatgattc tggagaacgg cggcgcgggt ccggcggttg gtacgctgat
    60481 gcgccagatc ctcgaccaca ttatgctggg tgataacaac accgatctgc ctgcggaaaa
    60541 tccagcggtt gccgcagcgg aggaccatta atcatgacgg ataatccgaa taaaaaaaca
    60601 ttctgggata aagtccatct cgatcccaca atgctgctga ttttactggc attgctggtt
    60661 tacagcgccc tggttatctg gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa
    60721 atcggccaaa tcgcgatggg tctggtcatc atggtggtga tggcgcaaat tcctccacgc
    60781 gtttatgaag gctgggcccc ctatctctat atcatctgta ttattttgct ggtggcggta
    60841 gatgctttcg gagccatctc taaaggcgct caacgctggc tggacctcgg tattgttcgt
    60901 tttcagccgt cggaaattgc caaaatagcc gtaccactga tggttgcgcg ctttatcaac
    60961 cgcgacgttt gcccgccatc gttgaagaat actggcatcg cgctggtgct gatatttatg
    61021 cccacgctgc tggtggctgc acagcctgac ctgggaacat caatcctcgt tgcgctttcc
    61081 ggtctgtttg tactgttcct ctctggcctt agctggcgtc tgattggcgt cgcagtagtg
    61141 ctggtagcgg cgttcattcc gattctgtgg ttcttcctga tgcatgatta ccagcgccag
    61201 cgcgtaatga tgctcctgga cccggaatca gacccactcg gcgcgggcta tcacattatt
    61261 cagtctaaaa ttgctattgg ctccggcgga ttacgcggca aaggctggct gcacggcact
    61321 cagtcacagc ttgaatttct ccccgaacgc catactgact ttatcttcgc ggtactggcg
    61381 gaagagctgg gattagtggg cattctgatt ctgctcgctc tctacattct gctgatcatg
    61441 cgcgggctgt ggatagccgc cagagcgcaa accacctttg gtcgcgtcat ggctggtggc
    61501 ttaatgctga tattattcgt ttatgtcttc gtaaatattg gtatggtaag cggtattctg
    61561 ccggttgtag gggttccgct cccactggtc agttatggag gatcggcgct aattgtgctg
    61621 atggctgggt tcgggattgt aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc
    61681 gtgtaagagg tgcgcaatgc gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct
    61741 cgcggcatgt acaagcgatg atggtcagca acagacggta agtgtaccgc agcctgcggt
    61801 atgtaacggc cccatagttg aaattagcgg ggcggacccg cgtttcgaac cactgaacgc
    61861 gacggcaaat caggattacc agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc
    61921 tcgatttagc caggcgggac tggcggcaat ctatgatgcc gaaccaggca gtaacctgac
    61981 ggcctctggc gaagctttcg atccgacaaa gctgacggcg gcccatccaa cgcttccgat
    62041 ccccagctac gccagaatca ctaacctggc taacgggcga atgatcgtgg tgcgcattaa
    62101 tgatcgcggc ccttacggca acgaccgcgt tatttcgctt tctcgcgcgg cagctgaccg
    62161 tcttaacacg tcaaacaaca ccaaagttcg tatcgatccg attattgtcg cccaggatgg
    62221 ttcgctttct ggtcctggta tggcttgtac cacagtcgcc aaacagactt acgccctgcc
    62281 tgcacctccc gatttaagcg gtggcgcggg aacaagttca gtgtctggcc cgcagggtga
    62341 cattcttccg gtcagtaatt cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac
    62401 cagtagcggt ttcctcggcg ctccaacgac cttagcgcct agtgtactgg aaggcagcga
    62461 accgacgcct gcgccacagc ccgttgttac agcttcgtcg acaacgcctg caacctcgcc
    62521 tgcaatggtg acaccgcaag ccgcctcgca aagcgccagc ggcaacttta tggtgcaagt
    62581 cggggccgta agcgatcagg ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt
    62641 cggcgtcccc ggtcgcgtaa ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt
    62701 cgccagcaaa gccgaagcca gtaccttgca gcaacgtttg caaaccgaag cccaattaca
    62761 gtcatttatt actaccgcgc agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa
    62821 atgacgttaa caaagtcacg cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt
    62881 agggcacttt tttaattcca tcacggatgt cgtagttcag accatgaata ccattttttc
    62941 cgctcgtatc atgaagcgcc tggcgctcac cacggctctt tgcacagcct ttatctctgc
    63001 tgcacatgcc gatgacctga atatcaaaac tatgatcccg ggtgtaccgc agatcgatgc
    63061 ggagtcctac atcctgattg actataactc cggcaaagtg ctcgccgaac agaacgcaga
    63121 tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc agttacgtta tcggccaggc
    63181 aatgaaagcc ggtaaattta aagaaactga tttagtcact atcggcaacg acgcatgggc
    63241 caccggtaac ccggtgttta aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt
    63301 tccggtttct cagctgatcc gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc
    63361 catggccgat tttgccgctg gtagccagga cgcttttgtt ggcttgatga acagctacgt
    63421 taacgcactg ggcctgaaaa atacccactt ccagacggta catggtctgg atgctgatgg
    63481 tcagtacagc tccgcgcgag atatggcgct gatcggccag gcattgatcc gtgacgtacc
    63541 gaatgaatac tcgatctata aagaaaaaga atttacgttt aacggtattc gccagctgaa
    63601 ccgtaacggc ctgttatggg ataacagcct gaatgtcgac ggcatcaaaa ccggacacac
    63661 tgacaaagca ggttacaatc ttgttgcttc tgcgactgaa ggccagatgc gcttgatttc
    63721 tgcggtgatg ggtggacgta cttttaaagg ccgtgaagcc gaaagtaaaa aactgctaac
    63781 ctggggcttc cgtttcttcg aaaccgttaa cccactgaaa gtaggtaaag agttcgcctc
    63841 tgaaccggtt tggtttagtg attctgatcg cgcttcgtta ggggttgata aagacgtgta
    63901 cctgaccatt ccgcgtggtc gcatgaaaga tctgaaagcc agctatgtgc tgaacagcag
    63961 tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga actatcaact tccagcttga
    64021 tggcaaaacg atcgagcaac gcccgctggt tgtgttgcaa gaaatcccgg aaggtaactt
    64081 cttcggcaaa atcattgatt acattaaatt aatgttccat cactggtttg gttaaaaatt
    64141 gaacacttga aagtgtaatt tccgtcccca tatactaagc atcagtaaaa aaactcccgc
    64201 cttctggcgg gagttgctat ttaattacgt tacgccggag ctgacatgaa aaccaaactt
    64261 aacgaactgc ttgaattccc tactcctttt acttacaaag ttatggggca ggcgttacct
    64321 gagctggttg atcaggtggt tgaagtggta cagcgccatg cgccaggtga ctacacccca
    64381 acggtaaaac caagcagcaa aggcaactac cactcggtat ctatcactat caacgccact
    64441 catatcgagc aggttgaaac actgtatgaa gaactgggca aaatcgatat tgtccgcatg
    64501 gttctgtaac tcgcttctcc gttacccggc tcctggtcgg gtaactcccc tctcctcgct
    64561 gtgatatact tttcccccac ttttactcat tctccacgga gatgccgttt tgtatcagga
    64621 taaaattctt gtccgccagc tcggtcttca gccttacgag ccaatctccc gggctatgca
    64681 tgaattcacc gatacccgcg ataatagtac ccttgatgaa atctggctgg tcgagcacta
    64741 tccggtattc acccaaggtc aggcaggaaa agcggagcac attttaatgc cgggtgatat
    64801 tccggtgatc cagagcgatc gcggtgggca ggtgacttat cacgggccgg gacaacaggt
    64861 gatgtatgtg ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt
    64921 gcttgagcaa acagtggtga ataccctggc tgaactgggt atagaagcgc atcctcgggc
    64981 tgacgcgcct ggtgtctatg tcggggaaaa gaaaatttgc tcactgggtt tacgaattcg
    65041 acgcggttgt tcattccacg gtctggcatt aaacgtcaat atggatcttt caccattttt
    65101 acgtattaat ccttgtgggt atgccggaat ggaaatggct aaaatatcac aatggaaacc
    65161 cgaagcgacg actaataata ttgctccacg tttactggaa aatattttag cgctactaaa
    65221 caatccggac ttcgaatata ttaccgctta attccataca tcaatggccc aatttacact
    65281 gggtcattac tcataatacc tgctaatgct ttacatctcc ttcgcgtttg tatattttct
    65341 ttatcacttg cagaaatgcg gtgccacgcc ttgctccgta actcacccca ttgattaacc
    65401 tattgaaata gttgattatg gtgagcaaaa gcgttccgtt ccaattagac ataagattat
    65461 tgtaattttt taaatattat tttcccatga ataaaattgg agtaagtgcg tggatagtaa
    65521 taatcaaatt gaaccctgtt taagcagaaa gtcgtcagaa ggtaaaccac aaatatttac
    65581 aaccttacga aatattgatc ttaaccttct gactattttt gaagctgtat atgtacataa
    65641 agggatcgtt aatgcagcga aagtgcttaa tctgaccccc tcggcaatca gtcagtctat
    65701 tcagaaactg cgcgttatat tccctgaccc attgtttatt cgcaaaggcc agggtgtcac
    65761 tcctaccgca tttgcgatgc atctacatga gtatatcagt cagggccttg agtccattct
    65821 tggcgcgctg gatatcgaag gaagctatga taagcaacga acgataacta ttgcgaccac
    65881 tccctcggtc ggagccctgg tccttcctgt catctatcgg gcgattaaaa ctcactatcc
    65941 gcagctttta ctgcgcaacc cgcccatcag cgacgcggaa aaccaactca gtcagtttca
    66001 aaccgatctc atcatcgata acatgttttg caccaatcgt acggtgcaac atcatgttct
    66061 gttcaccgac aatatggtgt taatttgccg caagggaaat ccactactct ctttagaaga
    66121 tgacagagag actatcgaca acgctgcgca tgtacttctg ttaccggaag ggcaaaattt
    66181 cagcggtctg cggcagagag ttcaagagat gtttccggac cggcaaatca atttcaccag
    66241 ctacaacatt ttgacaatcg ctgcactggt tgccaacagt gacatgttag cgattattcc
    66301 cagccgtttt tataacctgt ttagtcgttg ctggccgctg gaaaaactgc cttttccgtc
    66361 cttaaatgag gagcaaatag atttctctat ccactacaac aaattcatcc tgcgtgatcc
    66421 catcctgcac ggagtaatag atgttatccg taatgcattt tgaaaaagta ttgttacagc
    66481 cagatcacag cgcagaacga taaaatggca caatccaaca acaattttac attttcgcga
    66541 ccgctttggc tgctttcagg tccgtttcaa tgatatactg ccagtcgtta attcaaaaat
    66601 agttgataat tacaacaatc tattgaattg aaacgctttc cttcgtaatt cgcaactgga
    66661 acacgcacgc tatgagtaaa cccattgtga tggaacgcgg tgttaaatac cgcgatgccg
    66721 ataagatggc ccttatcccg gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca
    66781 agccggaatg gatgaaaatc aagcttccag cggactctac acgtatccag ggcatcaaag
    66841 ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga agcctcctgc cctaacctgg
    66901 cggaatgctt caaccacggc acagcaacgt ttatgatcct cggcgctatt tgtacccgcc
    66961 gttgtccgtt ctgtgatgtt gcccacggtc gcccggtagc tcctgatgcc aatgaaccag
    67021 tgaaactggc gcagaccatt gccgatatgg cgctgcgtta tgtggttatc acctccgttg
    67081 accgtgatga cctgcgcgat ggcggtgccc aacactttgc ggattgtatt actgccattc
    67141 gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc ggatttccgc ggtcgaatgg
    67201 atcgtgctct ggatattctg actgcaacgc ccccagatgt gttcaaccat aacctggaaa
    67261 acgtaccgcg tatttaccgg caggtacggc ctggcgcaga ttacaactgg tcgctgaagc
    67321 tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac caagtctggt ctgatggtgg
    67381 gtctgggtga aaccaatgaa gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg
    67441 tgacgatgtt aacgctgggg caatatttgc agccaagccg ccatcacctg ccggttcaac
    67501 gttacgttag cccggatgag ttcgacgaaa tgaaagccga agcgctggcg atgggcttta
    67561 cccatgctgc atgcggtccg tttgtccgct cttcttacca cgccgatttg caggcgaaag
    67621 gaatggaagt taagtaatcc tgtaataatt acttacatct gtctgataaa aaacccgctt
    67681 tttgaagcgg gttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
    67741 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
    67801 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
    67861 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
    67921 ggtaatacta atctcaccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
    67981 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
    68041 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
    68101 ttgtttaaga tgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
    68161 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
    68221 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
    68281 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccacgaacc
    68341 caggcggcag gaagtaccag gatttcagct ccctgtaaag cctgtgccag cgccagctct
    68401 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccttccac ctccagtaac
    68461 ggagctattt cattaccagc atcaacatgg cgtgattcct gaatggcaaa tgcatcatag
    68521 agatgcagtt tggcataacg ggcgacgatg tttcctgcct gaagtgccac cagcatattc
    68581 catgcgcgcc ccggcgttga aggaagatga atcgtcagaa ttgtcgtcat catgttacgt
    68641 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
    68701 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac aaacagcgat
    68761 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
    68821 acagatgtaa cagcaaactg tcctgcagca accagcattg ccacctcctg tcagagtcgt
    68881 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
    68941 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
    69001 agcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
    69061 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
    69121 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
    69181 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
    69241 ctgggcatta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
    69301 attctggctg ttttcggcct caaccgtaca ctaaagacca aaaaaacccg ctgattaagc
    69361 gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc agaagggcct
    69421 ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt tttaaaacca
    69481 ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc cggagtaatg
    69541 aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt agacatcaaa
    69601 attaccttta catgaaaaat agacacaaat gctgtgtcgg ttatcagtac accaattgtg
    69661 gtacgctttg tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta tttttgatgt
    69721 tgcttatttt agttattgat tttaaagaag ttactaaaac ttcatttgtc tcaaaactga
    69781 aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat gtaggtcatc
    69841 tgaaaagtca ctgggccata acccacggag gccaatggca gtagaaccgg gagagggatg
    69901 taattccagt tatcgcgtgc cgttacgcca gcggtgaatc ccagacctaa atgaaaattt
    69961 tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg ttcccatttg
    70021 ttccacgagt ccttaaatgc catggcatac aggccatgcc agtttccttt ttcatcccaa
    70081 cgcgacaggc caaaaccgcc accccacggt cgctcgttat agcgatcggt tttttctttg
    70141 tcgtaagcga aacgtgcatg ccaggtgatg gcaggaatat ataaatcata atgttcaggc
    70201 tgttgccagg tttgtgcaat attttctcta aacgttgtca tcctctcatc tgcgttagca
    70261 aaaactttac caacgctgat taactgaata aaaacaaagg aaaagatagc gacatattta
    70321 ctcacgttca tttgtgacca taaaacattt atcaaaaatc tactactagc atagcaaagc
    70381 tacaattaac ataaccttaa tagacccaac ataaagaata atctgaatag ctgctctatt
    70441 tatttccgct tgttcaagtt aaatatttcc gcgcatcgat taaagatgat gcgtcgtttt
    70501 ctgcgatggg aatagtcaaa aaagaaaaac caagtctttt ttgatgacaa aatgcaatca
    70561 aggaaaaatt attttatttt tttaacgaga tacaacaatc atcttaacga agtatataat
    70621 atatatcgtc aaccatgctc atattagatt attttttgag cgcacagccg cagctaacac
    70681 cattgcaatt aacaaatttg catcaatcca ccatcaattt acacacatta ttatgtgata
    70741 attgccaacc gctaaatatg cgttttgtta tctatgtata aaaacagcaa cttcaatgtc
    70801 ttaatggcag tttttcttga ttttaatcag cattcatcgc caatttattg ggcatatttt
    70861 ttccttaagc tttaggaagt ttttattatt tactttgggc ctggagacag gaaaaattat
    70921 gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtgg ctcgctacat
    70981 cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat tgctgattat
    71041 cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag gctacagcgc
    71101 cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg acctcggcat
    71161 gatgattatg atgctgtgtg gctttgccgc ttacatgacc catatcggcg cgaatgatat
    71221 ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc tgctgatgat
    71281 tgccgcctat tttgttgctt gtctgatgtc actggccgtc tcttccgcaa ccggtctggg
    71341 tgttttgctg atggcaaccc tgtttccggt gatggtaaac gttggtatca gtcgtggcgc
    71401 agcagctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt caggggatgt
    71461 ggtgctggcg gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca aaacgacgct
    71521 gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct ggcaacgtta
    71581 tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa tcaccaccac
    71641 tgcccccgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac tgatttttga
    71701 cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta tgctgattgc
    71761 ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg gtctggaagt
    71821 ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg ttgccgctgg
    71881 ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt ctatcgctac
    71941 ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga ccatgctggc
    72001 ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga tgatcccgaa
    72061 actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc tgcaggcgtc
    72121 aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg ccgggatggc
    72181 gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg ttgggctagt
    72241 gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg tcacagacaa
    72301 gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc actgtggtat
    72361 atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag atggcggctg
    72421 gcgcaatatt caagataacg cctggcagtg gtgcggctga tggtcagtgc ctgcgccacc
    72481 gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc gtttagcgtc
    72541 aaggggtcaa tgccggtcgg cagttcgtct ttgggttcac cgcgagcata ggcattaaac
    72601 atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg tttacgctgg
    72661 cggaaacggg ttagcgtttg ccccagtgta ttgattcact tgaagtacga aaaaaccggg
    72721 agaacattgg attattcggg atctgatggg attagatttg gtggggcttg caagcctgta
    72781 gtacaaattt taggcgttaa ccaatgaaac gcgaaagata gtaaaaaatt gcttttgttt
    72841 cattgaaaaa acgaaaaaca aaaacactat aaatcatttc aatcatagct tcaaaaaacg
    72901 ttcaaaaccg ataacaacca ggctgtcacc aaatgactca tatcacaaat cagctcatgc
    72961 cgtttaggta tgttacatgt gtgattatgt gaggtgaagt atgttttagc tggctcatgg
    73021 ttgttatacg gcttttttcg catcttgtgg ttccaacaaa ggagctacag gctcttcctg
    73081 tttatgaaca ccatacattg acaaaatctc ttggcgagtt tctggaggta atttatgcag
    73141 cgccaaagct gtttgtagtg tttggcgatc aatacccagc gttgccaata gggtgtcaac
    73201 ggagatggag gggcgttctt gcattaatcc ggaaagccca tatttagcca tgtttagctc
    73261 tatctctcgg acttcatttt ctcctagttt attgattaac agggagaaca ttttcaatgt
    73321 atcagcattc atttcttcga tagcaatttt ttgcatagcc ggtattgccg ggatatattc
    73381 aaccccaggc ccgcgacgcc cttcttgtga acgcttcggc catccttctt tctttgctcg
    73441 tttatgcacc ccctgtattg tcccaggcat tccatccagt tcagtaattt gtttgggtgt
    73501 catccactgg tttatctcat ttgcgctttt cataaactaa gtttaccaga tttatctata
    73561 taaaaatcaa tggattacaa aataaaccac attaactgat ataaaccggt tttgtttatt
    73621 tggtaatgta taaaacagat aatcaagacg aattatttgt tttggtaacc ctttatcata
    73681 cgcaatatgg ttggtgtgcg aaagatgagt aacgattggc atcgggccga cgtgattgcg
    73741 gccctcaaaa aaacaggaac ccctctggca aaattgtcca gacgttcagg ttattcaacc
    73801 gggacgcttg caaatgcatt agtcaggcca tggcctaaag gtgaacgcat tattgctgac
    73861 gccttaaaca tgaaacctga ggaaatttgg cctagtagat atctaaggga tcacaatgag
    73921 agagtggata tcagcaattg aatgcatggg tttatctggt ttacctggtt cattaatggg
    73981 ggttcacaaa agagctcaga gagaaggatg gctcaagaga agaagagaag gaattagagg
    74041 ggttgcttac gaataccata tatcttctct tcctaacatt gctcgagctg aagttttgct
    74101 ccaacaagga aaatttaaaa ccagtcaggg atgttttgaa atcgcccgcc ccacgctgga
    74161 agctcatgat tatgatcgtg aggcactatg gagcaaatgg gataaagcca gcgattccca
    74221 gcgcagcctg gctgaaaaat ggttgccctc aattcaggcc acagacgaaa tgctgaacca
    74281 ggggatttca acgaaaacgg cttttgcgac cgttgcaggg cattaccagg tcagcgcatc
    74341 cactttgcgg gacaagtatt accaggtaca gaagtttgcg aagcctgact gggcggctgc
    74401 acttgttgat ggacgtggag catcccgtcg caatgttcac aaaagtgaat ttgacgagga
    74461 tgcctggcag tttctgattg cagattatct gcgaccggaa aaacccgcct tccgcaaatg
    74521 ttatgagcgt ctggaactgg cagcccgcga gcatggctgg agtattccct cccgtgccac
    74581 ggcctttcgc cggattcagc aactggacga ggcaatggtt gttgcctgtc gtgaaggtga
    74641 acatgcactg atgcatctga taccggcaca gcagcgaact gtggaacacc tggacgccat
    74701 gcagtggatc aacggcgacg gttatctgca taacgtcttt gtacgctggt ttaacggtga
    74761 tgtgatccgt ccgaaaacat ggttctggca ggatgtgaaa acccgaaaaa ttctgggctg
    74821 gcgctgcgat gtgagcgaga acattgattc aattcgcctc tcgttcatgg atgttgtgac
    74881 tcgctacagt atcccggagg attttcacat caccattgat aacacccgtg gtgctgcgaa
    74941 taaatggctg acgggaggcg cgcccaatcg ctaccgcttt aaggtaaaag aggacgatcc
    75001 aaaaggactg tttttactga tgggtgcgaa aatgcactgg acaagcgttg ttgccggtaa
    75061 aggctggggc caggcaaaac ctgttgaacg tgctttcggt gttggtgggc ttgaggaata
    75121 cgttgataag catccggcac tggctggcgc atatacgggg ccaaatccgc aggcaaaacc
    75181 tgataactat ggcgaccacg ctgttgatgc agagctgttt ctgaaaacct ttgccgaagg
    75241 tgtggcgatg ttcaatgcca gaacaggccg tgaaacagaa atgtgcgggg gcaaactctc
    75301 gtttgatgat gttttcgagc gtgaatacgc cagaacgatt gtgcgtaagc caaccgaaga
    75361 acaaaaacgg atgctgttac tgcctgccga ggcggtgaac gtttcacgca aaggcgagtt
    75421 tacgcttaaa gttggcggct cccttaaagg cgcgaaaaac gtttattaca acatggcatt
    75481 aatgaatgcc ggagtgaaaa aagttgtggt cagatttgat ccacagcagt tacacagcac
    75541 ggtttattga g
//
LOCUS       DAECNO010000005        70704 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.005, whole genome shotgun sequence.
ACCESSION   DAECNO010000005 DAECNO010000000
VERSION     DAECNO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.005"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..794
                     /locus_tag="KKO08_000343"
     CDS             <1..794
                     /locus_tag="KKO08_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6316736.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="KKO08_000344"
     CDS             805..1896
                     /locus_tag="KKO08_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6316737.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="KKO08_000345"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="KKO08_000345"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBD6316738.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="KKO08_000346"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="KKO08_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBD6316739.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="KKO08_000347"
     CDS             4858..5253
                     /locus_tag="KKO08_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBD6316740.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="KKO08_000348"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="KKO08_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBD6316741.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="KKO08_000349"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="KKO08_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBD6316742.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="KKO08_000350"
     CDS             complement(6562..7602)
                     /locus_tag="KKO08_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD6316743.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="KKO08_000351"
     CDS             complement(7675..8310)
                     /locus_tag="KKO08_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBD6316744.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="KKO08_000352"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="KKO08_000352"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBD6316745.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="KKO08_000353"
     CDS             complement(8936..9379)
                     /locus_tag="KKO08_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBD6316746.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="KKO08_000354"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="KKO08_000354"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBD6316747.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="KKO08_000355"
     CDS             complement(9719..10222)
                     /locus_tag="KKO08_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD6316748.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="KKO08_000356"
     CDS             complement(10216..10740)
                     /locus_tag="KKO08_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBD6316749.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="KKO08_000357"
     CDS             10949..11944
                     /locus_tag="KKO08_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD6316750.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="KKO08_000358"
     CDS             11953..12831
                     /locus_tag="KKO08_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBD6316751.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="KKO08_000359"
     CDS             12912..13919
                     /locus_tag="KKO08_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBD6316752.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="KKO08_000360"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="KKO08_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBD6316753.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="KKO08_000361"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="KKO08_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBD6316754.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="KKO08_000362"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="KKO08_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBD6316755.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="KKO08_000363"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="KKO08_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBD6316756.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="KKO08_000364"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="KKO08_000364"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBD6316757.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="KKO08_000365"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="KKO08_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBD6316758.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="KKO08_000366"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="KKO08_000366"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBD6316759.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="KKO08_000367"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="KKO08_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBD6316760.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="KKO08_000368"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="KKO08_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBD6316761.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="KKO08_000369"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="KKO08_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBD6316762.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="KKO08_000370"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="KKO08_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBD6316763.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="KKO08_000371"
     tRNA            complement(27653..27729)
                     /locus_tag="KKO08_000371"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="KKO08_000372"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="KKO08_000372"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBD6316764.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="KKO08_000373"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="KKO08_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="KKO08_000374"
     tRNA            complement(31513..31599)
                     /locus_tag="KKO08_000374"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="KKO08_000375"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="KKO08_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBD6316765.1"
                     /translation="MYEALLVVFLIVAISLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="KKO08_000376"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="KKO08_000376"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBD6316766.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="KKO08_000377"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="KKO08_000377"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBD6316767.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="KKO08_000378"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="KKO08_000378"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBD6316768.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="KKO08_000379"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="KKO08_000379"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBD6316769.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="KKO08_000380"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="KKO08_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBD6316770.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="KKO08_000381"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="KKO08_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBD6316771.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="KKO08_000382"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="KKO08_000382"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD6316772.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="KKO08_000383"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="KKO08_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBD6316773.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="KKO08_000384"
     CDS             complement(41211..42176)
                     /locus_tag="KKO08_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD6316774.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="KKO08_000385"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="KKO08_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBD6316775.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="KKO08_000386"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="KKO08_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBD6316776.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="KKO08_000387"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="KKO08_000387"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBD6316777.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="KKO08_000388"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="KKO08_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBD6316778.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="KKO08_000389"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="KKO08_000389"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBD6316779.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="KKO08_000390"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="KKO08_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBD6316780.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="KKO08_000391"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="KKO08_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBD6316781.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="KKO08_000392"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="KKO08_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBD6316782.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="KKO08_000393"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="KKO08_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBD6316783.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="KKO08_000394"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="KKO08_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBD6316784.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="KKO08_000395"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="KKO08_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBD6316785.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="KKO08_000396"
     CDS             49714..50679
                     /locus_tag="KKO08_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBD6316786.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="KKO08_000397"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="KKO08_000397"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBD6316787.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="KKO08_000398"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="KKO08_000398"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBD6316788.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="KKO08_000399"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="KKO08_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBD6316789.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="KKO08_000400"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="KKO08_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBD6316790.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="KKO08_000401"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="KKO08_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBD6316791.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="KKO08_000402"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="KKO08_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBD6316792.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="KKO08_000403"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="KKO08_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBD6316793.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="KKO08_000404"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="KKO08_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBD6316794.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="KKO08_000405"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="KKO08_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBD6316795.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="KKO08_000406"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="KKO08_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBD6316796.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58504
                     /gene="yrbL"
                     /locus_tag="KKO08_000407"
     CDS             57878..58504
                     /gene="yrbL"
                     /locus_tag="KKO08_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBD6316797.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="KKO08_000408"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="KKO08_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="KKO08_000409"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="KKO08_000409"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBD6316798.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="KKO08_000410"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="KKO08_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBD6316799.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="KKO08_000411"
     CDS             complement(62540..63469)
                     /locus_tag="KKO08_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBD6316800.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="KKO08_000412"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="KKO08_000412"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBD6316801.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /gene="gltD"
                     /locus_tag="KKO08_000413"
     CDS             68617..70035
                     /gene="gltD"
                     /locus_tag="KKO08_000413"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBD6316802.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="KKO08_000414"
     CDS             70219..70698
                     /locus_tag="KKO08_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBD6316803.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17315 a        19177 c        17549 g        16663 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccgccattac cgtcatggtg cggtaaaaga agacgttacg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgca
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcagtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa cgccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctactgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt cgtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aagaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caagctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagttagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggctaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatca ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgataaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatctc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattca actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcacc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctggg
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaag
//
LOCUS       DAECNO010000006        67100 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.006, whole genome shotgun sequence.
ACCESSION   DAECNO010000006 DAECNO010000000
VERSION     DAECNO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.006"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="KKO08_000415"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="KKO08_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBD6316804.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="KKO08_000416"
     CDS             complement(1150..1236)
                     /locus_tag="KKO08_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBD6316805.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KKO08_000417"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="KKO08_000417"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD6316806.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KKO08_000418"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="KKO08_000418"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBD6316807.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3585..3629
                     /locus_tag="KKO08_000419"
     CDS             3585..3629
                     /locus_tag="KKO08_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316808.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KKO08_000420"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="KKO08_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBD6316809.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="KKO08_000421"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="KKO08_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBD6316810.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="KKO08_000422"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="KKO08_000422"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBD6316811.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="KKO08_000423"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="KKO08_000423"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBD6316812.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="KKO08_000424"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="KKO08_000424"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBD6316813.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="KKO08_000425"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="KKO08_000425"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBD6316814.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KKO08_000426"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="KKO08_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBD6316815.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KKO08_000427"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="KKO08_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBD6316816.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="KKO08_000428"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="KKO08_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBD6316817.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="KKO08_000429"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="KKO08_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBD6316818.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="KKO08_000430"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="KKO08_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBD6316819.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="KKO08_000431"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="KKO08_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBD6316820.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="KKO08_000432"
     CDS             complement(16981..17745)
                     /locus_tag="KKO08_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6316821.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KKO08_000433"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="KKO08_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBD6316822.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="KKO08_000434"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="KKO08_000434"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBD6316823.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="KKO08_000435"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="KKO08_000435"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBD6316824.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="KKO08_000436"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="KKO08_000436"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBD6316825.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="KKO08_000437"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="KKO08_000437"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBD6316826.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="KKO08_000438"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="KKO08_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBD6316827.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="KKO08_000439"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="KKO08_000439"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBD6316828.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KKO08_000440"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="KKO08_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBD6316829.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="KKO08_000441"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="KKO08_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBD6316830.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="KKO08_000442"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="KKO08_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBD6316831.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="KKO08_000443"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="KKO08_000443"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBD6316832.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="KKO08_000444"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="KKO08_000444"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBD6316833.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="KKO08_000445"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="KKO08_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBD6316834.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="KKO08_000446"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="KKO08_000446"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBD6316835.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KKO08_000447"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="KKO08_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBD6316836.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="KKO08_000448"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="KKO08_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBD6316837.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="KKO08_000449"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="KKO08_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBD6316838.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="KKO08_000450"
     CDS             complement(40421..41815)
                     /locus_tag="KKO08_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6316839.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="KKO08_000451"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="KKO08_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBD6316840.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="KKO08_000452"
     CDS             complement(42158..43444)
                     /locus_tag="KKO08_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD6316841.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="KKO08_000453"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="KKO08_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="KKO08_000454"
     CDS             complement(44451..45221)
                     /locus_tag="KKO08_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBD6316842.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /gene="caiT"
                     /locus_tag="KKO08_000455"
     CDS             45693..47207
                     /gene="caiT"
                     /locus_tag="KKO08_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBD6316843.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="KKO08_000456"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="KKO08_000456"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBD6316844.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="KKO08_000457"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="KKO08_000457"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBD6316845.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="KKO08_000458"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="KKO08_000458"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBD6316846.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..52247
                     /gene="caiD"
                     /locus_tag="KKO08_000459"
     CDS             51462..52247
                     /gene="caiD"
                     /locus_tag="KKO08_000459"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBD6316847.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52253..52843
                     /gene="caiE"
                     /locus_tag="KKO08_000460"
     CDS             52253..52843
                     /gene="caiE"
                     /locus_tag="KKO08_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBD6316848.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="KKO08_000461"
     CDS             complement(52929..53324)
                     /gene="caiF"
                     /locus_tag="KKO08_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBD6316849.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53586..56807)
                     /gene="carB"
                     /locus_tag="KKO08_000462"
     CDS             complement(53586..56807)
                     /gene="carB"
                     /locus_tag="KKO08_000462"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBD6316850.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56825..57973)
                     /gene="carA"
                     /locus_tag="KKO08_000463"
     CDS             complement(56825..57973)
                     /gene="carA"
                     /locus_tag="KKO08_000463"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBD6316851.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="KKO08_000464"
     CDS             complement(58429..59250)
                     /gene="dapB"
                     /locus_tag="KKO08_000464"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBD6316852.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="KKO08_000465"
     CDS             complement(59417..60331)
                     /gene="rihC"
                     /locus_tag="KKO08_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBD6316853.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="KKO08_000466"
                     /gene_synonym="lytB"
     CDS             complement(60397..61347)
                     /gene="ispH"
                     /locus_tag="KKO08_000466"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBD6316854.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="KKO08_000467"
     CDS             complement(61349..61798)
                     /gene="fkpB"
                     /locus_tag="KKO08_000467"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD6316855.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="KKO08_000468"
     CDS             complement(61885..62379)
                     /gene="lspA"
                     /locus_tag="KKO08_000468"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBD6316856.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="KKO08_000469"
     CDS             complement(62379..65195)
                     /gene="ileS"
                     /locus_tag="KKO08_000469"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBD6316857.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="KKO08_000470"
     CDS             complement(65238..66179)
                     /gene="ribF"
                     /locus_tag="KKO08_000470"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBD6316858.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66187..66405)
                     /locus_tag="KKO08_000471"
     CDS             complement(66187..66405)
                     /locus_tag="KKO08_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBD6316859.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66508..66771
                     /gene="rpsT"
                     /locus_tag="KKO08_000472"
     CDS             66508..66771
                     /gene="rpsT"
                     /locus_tag="KKO08_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBD6316860.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16290 a        18260 c        17267 g        15283 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaatataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggatttaa
    51661 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt tttgcgggat
    51721 taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc tacgcctttg
    51781 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    51841 tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg ctgcgtctgc
    51901 cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    51961 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    52021 ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    52081 tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    52141 gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc attgaagggc
    52201 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    52261 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    52321 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    52381 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    52441 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcatgg
    52501 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtaat
    52561 tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct ttgtcaaagc
    52621 aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    52681 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    52741 cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg aggaaaatcg
    52801 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcgacc
    52861 tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc ggtatcaacg
    52921 agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg ctcgcggtta
    52981 agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac taatttttcc
    53041 cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac tttaaccagt
    53101 cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac ttcgcagctt
    53161 atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct gtgttcaata
    53221 tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat catccattcg
    53281 gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac tccagtttca
    53341 caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca tattaaaatt
    53401 gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa tcctgcttat
    53461 gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa aacggtcctc
    53521 atcagagaac cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca
    53581 cgctattatt tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg
    53641 gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta
    53701 tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg
    53761 gtgttgatga tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct
    53821 tcatgcacct tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg
    53881 ccgtgggtcg catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg
    53941 cgttctttat cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag
    54001 ttgctgccca gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact
    54061 tccccggtag agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac
    54121 ggcagcacca cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc
    54181 tcagccagcg atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg
    54241 gctttggaga cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact
    54301 tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc gcacctgcaa ttcgaaggcc
    54361 agtttctgca cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc
    54421 agcgaacatg cggagtcacc ggagtgcaca cccgcctgct cgatgtgctc catgatgccg
    54481 ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca
    54541 aggaaatgat ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag
    54601 cgacgcaggt cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac
    54661 ggacgcacca ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca
    54721 gtaacggtgg cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt
    54781 tcacggtctt ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg
    54841 gcttccagtg cacgcgccag tttcagcggg gtctgaccgc cgtactgaac gataacgcct
    54901 ttcggcttct caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag
    54961 aggcgatcgg aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg
    55021 gtttcgtaac cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg
    55081 ataccctgac cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta
    55141 gacggattcg cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca
    55201 aactctgccg cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc
    55261 agcttacgga tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag
    55321 cctttgcgtt tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc
    55381 actttctctt ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta
    55441 gtcaggttga agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag
    55501 atacgctctg cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca
    55561 tccaggctca ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt
    55621 tgcagggatt cctgctgcgt gcgaccaatc gccatcactt cgccaaccga tttcatctga
    55681 gtggtcagac ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta
    55741 accacatagt cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc
    55801 atcagttcgt ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa
    55861 ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca
    55921 atcaggcgac cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg
    55981 ccgatttcac gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcggtc
    56041 agcgtttggg ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag
    56101 ttttcgatag agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg
    56161 tactctttcc agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc
    56221 agaccgcggg cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta
    56281 ccgcccatgg taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc
    56341 agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc
    56401 atcgctacgt cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca
    56461 atcatggtga caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc
    56521 agcgccgtct gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc
    56581 ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg
    56641 gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg
    56701 gctttacatg cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg
    56761 cccgcaccca gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc
    56821 ctgattactt agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg
    56881 gcgcggcgtc gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggct
    56941 tatcgatgcg atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca
    57001 ggtttgcagg taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca
    57061 ccacgttttt ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt
    57121 tgacagtctt cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata
    57181 ccggaatatc ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg
    57241 ggtcgccagg accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg
    57301 cagaagtttg cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga
    57361 tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt
    57421 tcgcttctgg caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg
    57481 cggtggtcac ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt
    57541 ctaacgccag cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt
    57601 tctcgcgcag taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat
    57661 ggcgcttcag gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca
    57721 ggtcgcgaat caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag
    57781 tgccgacatt gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat
    57841 cagtgaggat ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg
    57901 aacctgttgc ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg
    57961 ctgacttaat caaaacaccc tccagagaat actcactcac tttatttgca tattaattca
    58021 cagtgatgac gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca
    58081 ttctggagat atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg
    58141 gctcatgcat gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa
    58201 cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg
    58261 gtcaaaattt acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta
    58321 tagaaaattt aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa
    58381 ttaataaatt aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt
    58441 gagatcaagc acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc
    58501 cgatcttacc gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag
    58561 acgctcgcca atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac
    58621 ggtagcaaaa ccaatggtac ctggcacacg ttcaccggta tggccttcac gactgtagac
    58681 cgcgcaatct ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc
    58741 ggtgcctgac ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt
    58801 gtagtcaccc atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac
    58861 gctaaaattg gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt
    58921 accggcttcg tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa
    58981 agcgagatgg ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc
    59041 ttttatcgca tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc
    59101 accggcgtcg ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac
    59161 gccttctaat gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc
    59221 gatggcaacg cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac
    59281 cgttttcaga gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa
    59341 ttaatgataa acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc
    59401 gataacatgt gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc
    59461 cttttacatt cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga
    59521 tatcaaccac cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg
    59581 gcttgagggt gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc
    59641 gcagaccgct ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc
    59701 cggtgcgatt caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg
    59761 tgacatccag accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt
    59821 ctggatcggc agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac
    59881 cgcccatgat caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca
    59941 gcgcaatatt ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca
    60001 gggcatcccg aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa
    60061 agtcgtagcc agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta
    60121 ccagcggcac agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt
    60181 gcagggcatt gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt
    60241 gcaggtcgag ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg
    60301 ggtcagtatc gaggaagata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa
    60361 gaccggcatt ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac
    60421 acgcagctct ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc
    60481 ttcaccacca cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc
    60541 cgatgcgccc gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc
    60601 tttcgcatcg tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg
    60661 gttggagttg gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag
    60721 ggcgcgtacc gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc
    60781 gacaattttc gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtgt catccaccga
    60841 cagcgtggtt tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac
    60901 atcgtctggc gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt
    60961 cccttccact tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact
    61021 ggcgcgggca acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt
    61081 caaatcgcgg cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat
    61141 caggatcgcg ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg
    61201 caagctatcg accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc
    61261 gtaaatggcc agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc
    61321 acgcgggttg gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca
    61381 cttcaatatc aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg
    61441 agtcgccgtt aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa
    61501 gcatgattgc gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact
    61561 gaatcaggtc cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt
    61621 tgtcgcccac tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc
    61681 ccaggcggaa cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt
    61741 cgagttttag cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc
    61801 aggtttgctc ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct
    61861 gtaggccgca tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc
    61921 cagcacgatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
    61981 gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
    62041 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
    62101 cgtggccttc gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc
    62161 ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata
    62221 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
    62281 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
    62341 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
    62401 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
    62461 ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac
    62521 gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag
    62581 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
    62641 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc
    62701 actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
    62761 agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
    62821 taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca
    62881 tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac
    62941 agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct
    63001 ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt
    63061 accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc
    63121 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
    63181 gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
    63241 tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg
    63301 gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc
    63361 gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
    63421 tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa
    63481 gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg
    63541 gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg
    63601 tcgagagtat ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
    63661 tcgagatccc accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc
    63721 agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt
    63781 acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg
    63841 atacgcgcct ggccccagtc cgggagccac tgcacgcctt tgatctcttt cagtgactgc
    63901 gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc
    63961 ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc
    64021 gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca
    64081 tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt
    64141 tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga
    64201 acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag
    64261 cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga
    64321 atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga
    64381 atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt
    64441 gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg
    64501 ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg
    64561 acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa
    64621 cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg
    64681 attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg
    64741 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
    64801 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc
    64861 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
    64921 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
    64981 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
    65041 atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc
    65101 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
    65161 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt
    65221 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
    65281 tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt
    65341 atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac
    65401 aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc
    65461 acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc
    65521 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
    65581 tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc
    65641 agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca
    65701 ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt
    65761 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga
    65821 aattttacgc gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc
    65881 gcaaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa
    65941 cgcagttttt cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc
    66001 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
    66061 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
    66121 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
    66181 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
    66241 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggagc aaatcacggg
    66301 aagaaactga ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag
    66361 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
    66421 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
    66481 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
    66541 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
    66601 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
    66661 aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
    66721 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt
    66781 tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg
    66841 atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta
    66901 tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa
    66961 tatctactca atctccagac tcccccacaa agaatatgga tattgtgata cacattgagg
    67021 tattaaactt aagaccactc tatttttgaa ttaaattcct atatacttta accattcata
    67081 aatatctcct cattagacct
//
LOCUS       DAECNO010000007        66140 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.007, whole genome shotgun sequence.
ACCESSION   DAECNO010000007 DAECNO010000000
VERSION     DAECNO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.007"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..88
                     /locus_tag="KKO08_000473"
     CDS             <1..88
                     /locus_tag="KKO08_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6316861.1"
                     /translation="VRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(236..715)
                     /gene="folK"
                     /locus_tag="KKO08_000474"
     CDS             complement(236..715)
                     /gene="folK"
                     /locus_tag="KKO08_000474"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBD6316862.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="KKO08_000475"
     CDS             complement(712..2130)
                     /gene="pcnB"
                     /locus_tag="KKO08_000475"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBD6316863.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="KKO08_000476"
     CDS             complement(2169..3095)
                     /gene="gluQRS"
                     /locus_tag="KKO08_000476"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBD6316864.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="KKO08_000477"
     CDS             complement(3132..3587)
                     /gene="dksA"
                     /locus_tag="KKO08_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBD6316865.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="KKO08_000478"
     CDS             complement(3765..4469)
                     /gene="sfsA"
                     /locus_tag="KKO08_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBD6316866.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="KKO08_000479"
     CDS             complement(4484..5014)
                     /gene="thpR"
                     /locus_tag="KKO08_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBD6316867.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5088..7517
                     /gene="hrpB"
                     /locus_tag="KKO08_000480"
     CDS             5088..7517
                     /gene="hrpB"
                     /locus_tag="KKO08_000480"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBD6316868.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7713..10247
                     /gene="mrcB"
                     /locus_tag="KKO08_000481"
     CDS             7713..10247
                     /gene="mrcB"
                     /locus_tag="KKO08_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBD6316869.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10467..12710
                     /gene="fhuA"
                     /locus_tag="KKO08_000482"
     CDS             10467..12710
                     /gene="fhuA"
                     /locus_tag="KKO08_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBD6316870.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12761..13558
                     /gene="fhuC"
                     /locus_tag="KKO08_000483"
     CDS             12761..13558
                     /gene="fhuC"
                     /locus_tag="KKO08_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBD6316871.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13558..14448
                     /gene="fhuD"
                     /locus_tag="KKO08_000484"
     CDS             13558..14448
                     /gene="fhuD"
                     /locus_tag="KKO08_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBD6316872.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14445..16427
                     /gene="fhuB"
                     /locus_tag="KKO08_000485"
     CDS             14445..16427
                     /gene="fhuB"
                     /locus_tag="KKO08_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBD6316873.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16462..17742)
                     /gene="hemL"
                     /locus_tag="KKO08_000486"
     CDS             complement(16462..17742)
                     /gene="hemL"
                     /locus_tag="KKO08_000486"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBD6316874.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17967..19388
                     /gene="clcA"
                     /locus_tag="KKO08_000487"
     CDS             17967..19388
                     /gene="clcA"
                     /locus_tag="KKO08_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBD6316875.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19412..19477
                     /locus_tag="KKO08_000488"
     CDS             19412..19477
                     /locus_tag="KKO08_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316876.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19470..19814
                     /gene="erpA"
                     /locus_tag="KKO08_000489"
     CDS             19470..19814
                     /gene="erpA"
                     /locus_tag="KKO08_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBD6316877.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19861..20484)
                     /locus_tag="KKO08_000490"
     CDS             complement(19861..20484)
                     /locus_tag="KKO08_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBD6316878.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20522..21322)
                     /gene="btuF"
                     /locus_tag="KKO08_000491"
     CDS             complement(20522..21322)
                     /gene="btuF"
                     /locus_tag="KKO08_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBD6316879.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21315..22013)
                     /gene="mtnN"
                     /locus_tag="KKO08_000492"
     CDS             complement(21315..22013)
                     /gene="mtnN"
                     /locus_tag="KKO08_000492"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBD6316880.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22097..23614
                     /gene="dgt"
                     /locus_tag="KKO08_000493"
     CDS             22097..23614
                     /gene="dgt"
                     /locus_tag="KKO08_000493"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBD6316881.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23744..25168
                     /gene="degP"
                     /locus_tag="KKO08_000494"
     CDS             23744..25168
                     /gene="degP"
                     /locus_tag="KKO08_000494"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBD6316882.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25323..26479
                     /gene="cdaR"
                     /locus_tag="KKO08_000495"
                     /pseudo
     CDS             25323..26479
                     /gene="cdaR"
                     /locus_tag="KKO08_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26568..26954)
                     /locus_tag="KKO08_000496"
     CDS             complement(26568..26954)
                     /locus_tag="KKO08_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBD6316883.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27268..28092)
                     /gene="dapD"
                     /locus_tag="KKO08_000497"
     CDS             complement(27268..28092)
                     /gene="dapD"
                     /locus_tag="KKO08_000497"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBD6316884.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28123..30795)
                     /gene="glnD"
                     /locus_tag="KKO08_000498"
     CDS             complement(28123..30795)
                     /gene="glnD"
                     /locus_tag="KKO08_000498"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBD6316885.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30857..31651)
                     /gene="map"
                     /locus_tag="KKO08_000499"
     CDS             complement(30857..31651)
                     /gene="map"
                     /locus_tag="KKO08_000499"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBD6316886.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32019..32744
                     /gene="rpsB"
                     /locus_tag="KKO08_000500"
     CDS             32019..32744
                     /gene="rpsB"
                     /locus_tag="KKO08_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBD6316887.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33002..33853
                     /gene="tsf"
                     /locus_tag="KKO08_000501"
     CDS             33002..33853
                     /gene="tsf"
                     /locus_tag="KKO08_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBD6316888.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34000..34725
                     /gene="pyrH"
                     /locus_tag="KKO08_000502"
     CDS             34000..34725
                     /gene="pyrH"
                     /locus_tag="KKO08_000502"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBD6316889.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35017..35574
                     /gene="frr"
                     /locus_tag="KKO08_000503"
     CDS             35017..35574
                     /gene="frr"
                     /locus_tag="KKO08_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBD6316890.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35666..36862
                     /gene="ispC"
                     /locus_tag="KKO08_000504"
     CDS             35666..36862
                     /gene="ispC"
                     /locus_tag="KKO08_000504"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBD6316891.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            37051..37809
                     /gene="ispU"
                     /locus_tag="KKO08_000505"
                     /gene_synonym="uppS"
     CDS             37051..37809
                     /gene="ispU"
                     /locus_tag="KKO08_000505"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBD6316892.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37822..38679
                     /gene="cdsA"
                     /locus_tag="KKO08_000506"
     CDS             37822..38679
                     /gene="cdsA"
                     /locus_tag="KKO08_000506"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD6316893.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38691..40043
                     /gene="rseP"
                     /locus_tag="KKO08_000507"
     CDS             38691..40043
                     /gene="rseP"
                     /locus_tag="KKO08_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBD6316894.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40073..42505
                     /gene="bamA"
                     /locus_tag="KKO08_000508"
     CDS             40073..42505
                     /gene="bamA"
                     /locus_tag="KKO08_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBD6316895.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42627..43112
                     /gene="skp"
                     /locus_tag="KKO08_000509"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42627..43112
                     /gene="skp"
                     /locus_tag="KKO08_000509"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBD6316896.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43116..44141
                     /gene="lpxD"
                     /locus_tag="KKO08_000510"
     CDS             43116..44141
                     /gene="lpxD"
                     /locus_tag="KKO08_000510"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBD6316897.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYQTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44246..44701
                     /gene="fabZ"
                     /locus_tag="KKO08_000511"
     CDS             44246..44701
                     /gene="fabZ"
                     /locus_tag="KKO08_000511"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBD6316898.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44705..45493
                     /gene="lpxA"
                     /locus_tag="KKO08_000512"
     CDS             44705..45493
                     /gene="lpxA"
                     /locus_tag="KKO08_000512"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBD6316899.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45493..46641
                     /gene="lpxB"
                     /locus_tag="KKO08_000513"
     CDS             45493..46641
                     /gene="lpxB"
                     /locus_tag="KKO08_000513"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBD6316900.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46638..47234
                     /gene="rnhB"
                     /locus_tag="KKO08_000514"
     CDS             46638..47234
                     /gene="rnhB"
                     /locus_tag="KKO08_000514"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBD6316901.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47271..50753
                     /gene="dnaE"
                     /locus_tag="KKO08_000515"
     CDS             47271..50753
                     /gene="dnaE"
                     /locus_tag="KKO08_000515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBD6316902.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50766..51725
                     /gene="accA"
                     /locus_tag="KKO08_000516"
     CDS             50766..51725
                     /gene="accA"
                     /locus_tag="KKO08_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBD6316903.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51824..53965
                     /gene="ldcC"
                     /locus_tag="KKO08_000517"
     CDS             51824..53965
                     /gene="ldcC"
                     /locus_tag="KKO08_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBD6316904.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCPV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54022..54411
                     /locus_tag="KKO08_000518"
     CDS             54022..54411
                     /locus_tag="KKO08_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD6316905.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54476..55774
                     /gene="tilS"
                     /locus_tag="KKO08_000519"
     CDS             54476..55774
                     /gene="tilS"
                     /locus_tag="KKO08_000519"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBD6316906.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLKLPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55823..56083)
                     /gene="rof"
                     /locus_tag="KKO08_000520"
     CDS             complement(55823..56083)
                     /gene="rof"
                     /locus_tag="KKO08_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBD6316907.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56070..56270)
                     /locus_tag="KKO08_000521"
     CDS             complement(56070..56270)
                     /locus_tag="KKO08_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBD6316908.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56436..56981
                     /locus_tag="KKO08_000522"
     CDS             56436..56981
                     /locus_tag="KKO08_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBD6316909.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56978..57400
                     /gene="arfB"
                     /locus_tag="KKO08_000523"
     CDS             56978..57400
                     /gene="arfB"
                     /locus_tag="KKO08_000523"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD6316910.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57414..58124
                     /gene="nlpE"
                     /locus_tag="KKO08_000524"
                     /gene_synonym="cutF"
     CDS             57414..58124
                     /gene="nlpE"
                     /locus_tag="KKO08_000524"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBD6316911.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58279..59103)
                     /locus_tag="KKO08_000525"
     CDS             complement(58279..59103)
                     /locus_tag="KKO08_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBD6316912.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59156..60874)
                     /gene="proS"
                     /locus_tag="KKO08_000526"
     CDS             complement(59156..60874)
                     /gene="proS"
                     /locus_tag="KKO08_000526"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBD6316913.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60985..61692)
                     /gene="tsaA"
                     /locus_tag="KKO08_000527"
     CDS             complement(60985..61692)
                     /gene="tsaA"
                     /locus_tag="KKO08_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBD6316914.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61689..62093)
                     /gene="rcsF"
                     /locus_tag="KKO08_000528"
     CDS             complement(61689..62093)
                     /gene="rcsF"
                     /locus_tag="KKO08_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBD6316915.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62211..63026)
                     /gene="metQ"
                     /locus_tag="KKO08_000529"
     CDS             complement(62211..63026)
                     /gene="metQ"
                     /locus_tag="KKO08_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBD6316916.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63066..63719)
                     /gene="metI"
                     /locus_tag="KKO08_000530"
     CDS             complement(63066..63719)
                     /gene="metI"
                     /locus_tag="KKO08_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBD6316917.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63712..64743)
                     /gene="metN"
                     /locus_tag="KKO08_000531"
     CDS             complement(63712..64743)
                     /gene="metN"
                     /locus_tag="KKO08_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBD6316918.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64931..65506
                     /gene="gmhB"
                     /locus_tag="KKO08_000532"
     CDS             64931..65506
                     /gene="gmhB"
                     /locus_tag="KKO08_000532"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBD6316919.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65870..>66140
                     /locus_tag="KKO08_000533"
     rRNA            65870..>66140
                     /locus_tag="KKO08_000533"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15657 a        16372 c        18228 g        15883 t
ORIGIN      
        1 ggtacggaag tcactgtcgt tctcaaaatc ggtggagctg catgacaagg tcatcgggca
       61 ttatctgaac ataaaacact atcagtaagt tggagtcatt accggcgatg ggattgatat
      121 ttatctgcgg gaatttatat tatgtttctg caaaaatgtt taaactaaaa tttttattaa
      181 ggaaagagag gatgtatgat tttcagaaaa tatttatatt cgtaatataa ataaattacc
      241 atttgcttaa tttgtcaaat gctcttgtat gtaagacttc acgcaacgtc tccccatcag
      301 gaaacgccag ctccggtgca atctcaaaca gcggccacag cataaatcca cgattcttca
      361 tatcgtagtg cggaacggtc aggcgttcgg tatttatcac ttcattacca aacagcatga
      421 tatcgagatc cagcgtgcgt ggtccccagc gttcagcttt acgaacacga ccctgttgca
      481 gttctatgcg ctgtgtatgg ttgagtaact cttccggggc gggcgaagtt tcaagcgcca
      541 cggcggcgtt gaggtaatcg ggttgatctt gcggccccag cggtggggtg cggtaaaacg
      601 aagaaacggc aagaatgcgg ctttcaggga tatcgcctaa tgctttcagg gcagcattga
      661 cctgctccag cggagaggcc agattgctgc ctatggcaat atacgccact gtcatgtggt
      721 accctcacga cgcggtgcgc gtttgcgtgg acgacgagta cgacgacgcg gtgacggttc
      781 ttcatccagc tcgttaagca tccctttttg atctggtggt gcggaaacct ggaactcacc
      841 ccaccatttc accagacgct gcagttcagc attacgttca acttcagctc gcaaggccaa
      901 caggtcataa gccgcacgga acttaggatg ctccagcagt ttccatgcgc gtttaccctg
      961 acgacgggac atacgcaact gcaactgcca gatatcgcgg gttaacgtcg tcagacgttt
     1021 cgggattgcc agtgaacggc aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc
     1081 gtcgtgatag gtcaggccgc tttcctgggc gatcttctgt gccgtctcca gcagtgggta
     1141 ccagaacatg gcggcaaaca agaacgccgg gttcacgcgc atatcgttat ggatacgcgt
     1201 atcggtattc ttcagcacct gttcaatgat ccgctccatc gggctgtcgc cattttccgt
     1261 gaagtagcgg gtaatggtcg ggaacagcgg ctggaacaga tgatattcac acaacagctt
     1321 ataggtttcg taaccgtagc ccgcttgtag cagtttaagc gattcttcaa acaggcgtgc
     1381 cggtgggata tcgttcagca gggtagcgag gcgagggatc ggttctgcgg tttccgggct
     1441 gatgcgcata cccaatttgg cggcaaaacg taccgcgcgc agcatacgta ccggatcttc
     1501 acggtagcgc gtttccgggt taccaatcag acggataacg ccgtccttca gatccttcat
     1561 accaccaacg taatcacgga cggtaaaatc cgctacgctg taatacaggc tgttgatagt
     1621 gaaatcgcgg cgctgggcgt cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat
     1681 gccgttttgc ccgcgttggg aggtcgtgcg gtcgctgacg ttaccttcgt ggtgtccacg
     1741 gaaggtcgca acttcgataa tctccgggcc aaacattaca tgagccagac ggaaacggcg
     1801 acccaccagg cggcagttac ggaacagttt gcgcacctgc tcaggcgtgg cgttagtggt
     1861 tacgtcaaaa tctttcggct ttttgccaag taacaggtcg cgcacgccgc cgccaaccag
     1921 ccaggcttcg tatcccgctt tgttgagcct gtacattacc ttcagggcat tttcactgat
     1981 atctttgcgg gaaatagcat gctgctcacg cgggatcacc gtcacctgtg gacgggcgac
     2041 tgcctgttca gcctcgcttt cctcgcggct tagcaccttg cggcaaaaat tagcgactcg
     2101 ggtaaaaata gtacacctcg gtagtgtcaa acatcattca ggacaaaaaa atagcggcta
     2161 atcatagctc agcatgacgc atttgagaat gttgaattta caattgccga ctcgggcacg
     2221 gcggtcagca tccagttttt gacggctgac tgaaggattt gctcgacgct gaaatcctgc
     2281 cagtgtgctt ctgcctgctg ccccagaaat tgaagtgccg cgattagtac cgggcgtgga
     2341 tcgcctttcg gcaacgcagg cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta
     2401 agcgccagcg gcagatgaat gtaatcaggc actttccagc caaaaagctg gtacagcggg
     2461 atttgcctta ctgttggttc aatcagatca gccccacgca ctatttctgt aacgccctgg
     2521 aaatgatcat caaccacaac agccaggttg taggcgaaca acccatcacg gcgatgaatg
     2581 ataaaatctt cccgtgccag tttttcgtcg gcgtgaataa tgcctcgcag caggtcagta
     2641 aattgcgtga ccggatgctg ctggcggata cgcactgcgg cgttgtctgg tccatgatgc
     2701 aacacccggc aatgaccgtc gtaaataccg ccaatgcttt gaatacgcgc acgcgtacag
     2761 gtgcagtaat aacttagtcc ttgttcatgt aaccaggcga gtgcttcacg ataggcgtcg
     2821 tgacgttgcg attgccagag aacatcgccg tcccagtgca gaccgtaatg ttccagctgg
     2881 cgcaggatag tttctgcggc accgggaact tcacgaggcg ggtcgatatc ttctatgcgt
     2941 accagccagc gaccttgccg ggcgcgagcc tgcaaatagc tgccgagcgc ggcgatcaga
     3001 gagccaaaat gaagctcgcc ggaaggagag ggggcgaagc ggccaatata ctgtgtgtct
     3061 gtcatctctt tgaacaaaaa ataaggcggg agcatttccc gcctgtggta aacgtgatgg
     3121 aacggctgta attagccagc catctgtttt tcgcgaattt cagccagcgt tttgcagtcg
     3181 atgcacagat cggctgtcgg gcgcgcttcc agacggcgaa taccaatttc aacaccgcag
     3241 gattcgcagt agccgaaatc ttcgtcttcc acttttttca gcgtcttctc gatctttttg
     3301 atcagcttac gctcgcgatc gcggttacgc agttcgaggc tgaactcttc ttcctgggct
     3361 gcacggtcta ccgggtccgg gaagttggct gcttcatcct gcatatgtgt aacggtgcga
     3421 tcgacttcat ccctgagttg attacgccat gcttccagaa tacgacggaa gtgcgccagc
     3481 tgggcttcat tcatatactc ttcgcccggc ttctcctgat atggttccac cccagcgatg
     3541 gcgagaatac tcagggacga tgttttacgg ttttgccctt cttgcatgtt gcttctcctt
     3601 aacacgcact atcgatcccc atgttcgggg gaaaaatgag gccgctataa atagcagatg
     3661 cttttccgga tagcaattat ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat
     3721 ttgcgcacgc gaccagaatg taaattaacc agttacttac tttactacaa tgtaaccggc
     3781 agtgattttt taagagccat gccttcagca gaaatttccg ctttgtaagc cagaatttct
     3841 acccccctct gttgagcttc tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg
     3901 ggtgaaaacc gtgtaatggc tgaatgcagc acggcgaaaa aaataaccgc acgctggcct
     3961 tcagccgcta cgctcatcaa ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg
     4021 ggaaaatatc cctgttcgtt ctccgctaac gtaaccgatt tcacttcaat atagcagtct
     4081 ggacgcgaat ccgcctgcaa cataaagtca atacggctgc gttcggagcc gtattttact
     4141 tcgcttttca gcgagctata gcctgacagt tctgaaattg attcattaag gatagcctct
     4201 ttcgtcaacc tgttagccca aagcgtattg acgcaaataa atgcgccgct ctggctttga
     4261 gttaattccc aggtgtgtgg gtatttccgt ttggtgttgt ctgaagtcga ataccagacg
     4321 gtatcgccag gcgttgcaca accggtcatc gcacccgtat tcgggcagtg tagcgttaat
     4381 tcgcgaccat cgggtgtgat cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc
     4441 gcgcgctgta gagggggaga aaattccatt gcgacaatcc ttgttattgc gttagcgccc
     4501 agcgttttag cggcgtgtag cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga
     4561 actccgtcac cgcataagac cagttaaaac ccggcggcgg gaatgtcacc gcctcgctgg
     4621 cgtcgcgcaa taaggtaata tgtggatgaa acggacgatt gctttgaaaa caaccgctgc
     4681 gggcagcctg tgaacggagc atattcgcca gctggattaa gccgcgtgga ggctgacgca
     4741 tccctaacca caccacacgc gaacgcagcc attgtccggc gtcatcaagc gtgagtgtga
     4801 aaccaggttg acgaatccgt ccggctaaaa gagaaagcgc cttctctttc tctgcgctca
     4861 cttcgcctaa aaatgccaga gtcaggtgca aattatcggc ggcgaccgga cgtcccgcct
     4921 caggtgggaa gtgtgtggcg cgccaatgga taatctgttc gcggatttct gcaggtaagt
     4981 cgatagcaaa gaacagacgt tgcggttcag acatgtgagg cactcggtta tgaattaccg
     5041 cgatgctaca atgtggcgcg aagaatgtta accctctgga gcgttttgtg tcgtcgttgc
     5101 ccgttgctgc cgtcttacct gaattactta ccgctctcga ttgtgcgccg caggtattat
     5161 taagtgcgcc gaccggggcc gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc
     5221 ccggcattaa cgggaaaatt atcctgctgg agccgcgtcg tctggcggcg cgtaacgtcg
     5281 cgcaacggct ggcggagctg cttaacgaaa agccaggcga taccgttggc taccggatgc
     5341 gtgcgcaaaa ctgcgtcggg ctgaataccc gcctggaagt ggttaccgaa ggcgtgctga
     5401 cgcggatgat ccagcgtgac ccggaactga gcggtgttgg attggtgatc cttgatgagt
     5461 ttcatgagcg cagtttgcag gcggatttgg cgttggcgct gttactcgat gtgcaacaag
     5521 gtctgcgtga tgaccttaaa ctgctgatta tgtcggctac gctggacaat gaccgcttgc
     5581 agcaaatgct gccggaagcg cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac
     5641 gccgttattt accgctgccc gcgcatcagc gttttgacga agccgttgcg gtagccaccg
     5701 ccgaaatggt gcgtcaggaa agtggatcat tactgttatt tttacctggc gtcggagaaa
     5761 ttcagcgcgt gcaggaacaa ctggcttcgc gcatcggcag tgatgtggtg ctctgcccgc
     5821 tgtatggcgt gttgtcactg aacgatcagc gaaaagcgat cctcccggca ccgcaaggga
     5881 tgcgcaaagt ggtgctggcg accaatattg ctgaaaccag tttaaccatc gaaggtattc
     5941 gtctggtggt ggattgtgcc caggagcgtg tggcgcgctt tgatccgcgc acggggctta
     6001 cgcggttgat tactcaacgc gttagtcagg catccatgac gcagcgtgcc gggcgcgccg
     6061 ggcgtctgga gccgggtatc agcctgcatt taatcgccaa agaacaagca gaacgcgccg
     6121 cggcgcaaag tgagccggag atcttacaaa gcgatctttc cggtttgctg atggaattac
     6181 tgcaatgggg atgcagcgat ccggcgcaga tgagctggct ggatcaaccg ccaacggtga
     6241 atctactggc cgcgaaacgc ctgttacaaa tgctgggggc gctggagggt gaacggctta
     6301 gtgcgcaagg gcaaaaaatg gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg
     6361 ttaacgcaaa gagcgatgac gaagctgcta ccgcggcaaa aattgccgcc attctcgaag
     6421 agccgccacg gatgggcaat agtgacctgg gcgtggcgtt ttcgcgcaat caaccagcct
     6481 ggcagcaacg tagtcagcaa ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca
     6541 gttcgcttat cgcgccgcta cttgccgggg cgtttgccga tcgcattgct catcgccgtg
     6601 ggcaagatgg acgctatcaa ctggcaaaca gcatgggagc gatgctcgat gccgacgacg
     6661 cgctaagccg ccacgaatgg ttgatcgcac cgttattatt gcagggcagc gcctcgccgg
     6721 atgcgcggat tttactggcg ctaccggtcg atattgatga attagtacaa cgctgtccgc
     6781 agctggtgca gcaatctgac accgtggaat gggatgacgc gcaaggtacg ctgaaagcct
     6841 ggcgtcggct acaaatcggt cagttgacgg tgaaagtgca gccgctggcg aaaccgtcag
     6901 aagacgagtt gcatcaggcg atgcttaatg gcatccgtga taaaggttta agcgtgctca
     6961 actggacggc ggaagcggaa cagctacgct tgcgtttgtt atgcgccgca aagtggttgc
     7021 cggaatatga ctggccagcg gttgatgatg aaagtttgtt ggcgacgctg gaaacgtggc
     7081 tgctgccaca tatgaccggc gtacattcac tacgcggcct gaaatcactc gacatttatc
     7141 aggcactacg cggattactt gattggggaa tgcagcaacg tctggatagt gaattgcctg
     7201 cgcattacac tgtgccgacg ggaagccgga tcgccattcg ttatcatgaa gataacccgc
     7261 ccgcgctggc ggtgagaatg caggagatgt ttggtgaggc cactaatccg acgatcgccc
     7321 aggggcgcgt gccgctggtg ctgaagttgc tttcccctgc ccaaaggccg ctgcaaatca
     7381 cgcgtgattt gggggccttc tggaaagggg cgtatcgtga ggtgcaaaag gagatgaaag
     7441 ggcgttatcc caaacacgtc tggccggacg acccggcaaa cacggcaccg acgcgacgga
     7501 cgaaaaagta ttcgtaaggt tggttttctc cctctccctg tgggagaggg ccggggtgag
     7561 ggcatcagcg cgcacgttca ccctcaccct aaccctctcc ctcaagggag aggggaccga
     7621 tcgagcgcaa attttgagag atatcttctt ctgtcttgta acagaagaac agaaaatcgg
     7681 gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc gagccaattg
     7741 gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag
     7801 atgacgatga ttacgacgat tatgatgact atgaggatga agaaccgatg ccgcgcaaag
     7861 gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta tggctactgc
     7921 taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat ctcgatcaaa
     7981 aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc cgcggcagtt tatggccgaa
     8041 tggtcaacct tgaaccagat atgaccatca gcaagaacga aatggtgaag ctgctggagg
     8101 caacacagta tcgtcaggtg tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca
     8161 acagcattga gatgatccgc cgtccgtttg atttcccgga cagtaaagaa gggcaggtgc
     8221 gtgcgcgtct gacctttgat ggtgatcatc tggcgaccat cgtcaatatg gagaacaacc
     8281 gtcagttcgg tttcttccgt cttgatccgc gtctgatcac catgatctcc tcgccaaacg
     8341 gtgagcagcg tttgtttgtg ccgcgcagtg gtttccctga tttgctggtg gatactttgc
     8401 tggcgacaga agaccgtcat ttttacgagc atgatggaat cagtctctac tccattgggc
     8461 gtgcggtgct ggcaaacctg accgcaggtc gcacggtgca gggtgcgagt acgctgacgc
     8521 aacagctggt gaaaaacctg ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg
     8581 aagcttacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt cttgagctgt
     8641 atatgaacga ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc ttcccgctgg
     8701 caagcttgta ttactttggt cgcccggtag aagagctaag cctcgaccag caggcgctgt
     8761 tagtcggtat ggtgaaaggg gcgtccatct acaacccgtg gcgtaacccg aaactggcgc
     8821 tggagcgacg taatctggtg ctgcgtctgc tgcaacagca acagattatt gatcaagaac
     8881 tctatgacat gttgagtgcc cgtccgctgg gggttcagcc gcgtggtggg gtgatctctc
     8941 ctcagccagc ctttatgcag ctggtgcgtc aggagttgca ggcaaaactg ggcgataagg
     9001 taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc caggacgcgg
     9061 cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa acagcgtaag ttgagcgatc
     9121 ttgaaactgc gattgtggtc gtcgaccgct ttagtggtga agttcgtgcg atggtcggag
     9181 gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt
     9241 cccttgcaaa accagcgact tatctgacgg ctttaagcca gccgaaaatc tatcgcctga
     9301 acacgtggat tgccgatgcg ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac
     9361 cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt gatgctggtg gatgcgttga
     9421 cacgttcgat gaacgtgccg acggtaaatc tggggatggc gctggggctg cctgcggtta
     9481 cggagacctg gatcaaactg ggcgtaccga aagatcagtt gcatccggtt ccggcaatgc
     9541 tgctgggggc gttgaactta acgccaatcg aagtggcgca ggcattccag accatcgcca
     9601 gcggtggtaa ccgtgcaccg ctttctgcac tgcgttcggt aatcgcggaa gatggtaagg
     9661 tgctgtatca gagcttcccg caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga
     9721 cactatggac catgcagcag gtggtacaac gtggtacggg tcgtcagctt ggggcgaaat
     9781 acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta gatacctggt
     9841 ttgcaggcat tgacggcagc acggtgacca tcacctgggt aggccgtgat aacaaccagc
     9901 cgaccaaact gtatggagcc agcggggcaa tgtcgattta tcagcgttat ctggctaacc
     9961 agacgccaac gccgctgaat cttgttccgc cagaagatat tgcagatatg ggcgtggact
    10021 acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg accagcgatc
    10081 cgcaatcgct gtgccagcag agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc
    10141 agtcttctca gccgcagcaa cagccgcaac agcaacctgc tcagcaagag cagaaagaca
    10201 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga
    10261 aataccggat ggcgagttgc catccggtaa aataacatcc catctaagat attaaccctt
    10321 tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga
    10381 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatt
    10441 attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10501 cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac
    10561 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10621 aaagcgcatg ggggcctgct gcaactattg cggcgcggca gtcagctacc ggcactaaaa
    10681 ccgatacgcc gattcaaaaa gtgccacagt ctatttctgt tgtgaccgcc gaagagatgg
    10741 cgctgcatca gccgaagtcg gtaaaagaag cgcttagcta cacgccgggt gtctctgttg
    10801 gtacgcgtgg cgcatccaac acctatgacc acctgatcat tcgcggtttt gcggcagaag
    10861 gccaaagcca aaataactat ctgaatggcc tgaagttgca gggcaacttc tataacgatg
    10921 cggtcattga cccgtatatg ctggaacgcg ctgaaattat gcgtggcccg gtttccgtgc
    10981 tttacggtaa aagcagtcct ggcggtctgt tgaatatggt cagcaagcgt ccgaccaccg
    11041 aaccgctgaa agaagttcag tttaaagccg gtactgacag cctgttccag actggttttg
    11101 actttagcga tgcgttggat gatgacggtg tttactctta tcgcctgacc ggtcttgcgc
    11161 gttctgccaa tgcccagcag aaagggtcag aagagcagcg ttatgctatt gcaccggcgt
    11221 tcacctggcg tccggatgat aaaaccaatt tcaccttcct ttcttacttc cagaacgagc
    11281 cggaaaccgg ttattacggc tggttgccga aagagggaac cgttgagccg ctgccgaacg
    11341 gtaagcgtct gccgacagac tttaatgaag gggcgaagaa caacacctat tctcgtaatg
    11401 agaagatggt cggctacagc ttcgatcacg aatttaacga cacctttact gtgcgtcaga
    11461 acctgcgctt tgctgaaaac aaaacctcgc aaaacagcgt ttatggttac ggcgtctgct
    11521 ccgatccggc gaatgcttac agcaaacagt gtgcggcatt agcgccagcg gataaaggcc
    11581 attatctggc acgtaaatac gtcgttgatg atgagaagct gcaaaacttc tccgttgata
    11641 cccagttgca gagcaagttt gccactggcg atatcgacca caccctgctg accggtgtcg
    11701 actttatgcg tatgcgtaat gacatcaacg cctggtttgg ttacgacgac tctgtgccac
    11761 tgctcaatct gtacaatccg gtgaatcccg atttcgactt caatgccaaa gatccggcaa
    11821 actccggccc ttaccgcatt ctgaataaac agaaacaaac gggcgtttat gttcaggatc
    11881 aggcgcagtg ggataaagtg ctggtcaccc taggcggtcg ttatgactgg gcagatcaag
    11941 aatctcttaa ccgcgttgct gggacgaccg ataaacgtga tgacaaacag tttacctggc
    12001 gtggtggtgt taactacctg tttgataatg gtgtaacacc ttacttcagc tatagcgaat
    12061 cgtttgaacc ttcttcgcaa gttgggaagg atggtaatat tttcgcaccg tctaaaggta
    12121 agcagtatga agtcggcgtg aaatatgtac cggaagatcg tccgattgta gttactggtg
    12181 ccgtgtataa tctcactaaa accaacaacc tgatggcgga ccctgagggt tccttcttct
    12241 cggttgaagg tggcgagatc cgcgcacgtg gcgtagaaat cgaagcgaaa gcggcgctgt
    12301 cggcgagtgt taacgtagtc ggttcttata cttacaccga tgcggaatac accaccgata
    12361 ctacctataa aggcaatacg cctgcacagg tgccaaaaca catggcttcg ctgtgggctg
    12421 actatacctt ctttgacggt ccgctttcag gtctgacgct gggcaccggt ggtcgttata
    12481 ctggctccag ctatggtgat ccggctaact cctttaaagt gggaagttat acggtcgtgg
    12541 atgcgttagt gcgttatgat ctggcgcgag tcggcatggc gggctccaac gtggcgctgc
    12601 atgttaacaa cctgttcgat cgtgaatacg tcgccagctg ctttaacact tatggctgct
    12661 tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt tcgtttctaa tttctctttt
    12721 ggggcacgga tttccgtgcc catttcacaa gttggctgtt atgcaggaat acacgaatca
    12781 ttccgatacc acttttgcac tgcgtaatat ctcctttcgt gtgcccgggc gcacgctttt
    12841 gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc ggtctgattg gtcacaacgg
    12901 ttctggtaaa tccactctgc tcaaaatgct tggtcgtcat cagcctccgt cagaagggga
    12961 gattcttctt gatgcccagc cgctggaaag ctggagcagc aaagcgtttg cccgcaaagt
    13021 ggcttatttg ccgcagcagc ttcctccggc agaagggatg accgtgcgtg aactggtggc
    13081 gattggtcgt tacccgtggc atggcgcgct ggggcgcttt ggtgcggcag atcgcgaaaa
    13141 ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg gcgcatcggc tggtcgatag
    13201 tctctctggc ggcgaacgtc agcgggcgtg gatcgccatg ctggtggcgc aggatagccg
    13261 ttgtctgttg ctcgacgaac cgacttcagc gctggatatc gcccaccagg ttgatgtgct
    13321 ggcgctggtg caccgtttaa gtcaggaacg tggcctgacg gtcattgccg tgttgcacga
    13381 tatcaatatg gcggcacgct actgtgatta tctggtcgcc ctgcgcagtg gtgaaatgat
    13441 tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc ctcgaaatga tttatggcat
    13501 cccgatgggt attttgccgc atccggcggg tgctgcacct gtgagttttg tttattgatg
    13561 agcggcttac ctcttatttc gcgccgtcga ctgttaacgg cgatggcgct ttctccgttg
    13621 ttatggcaga tgaataccgc ccacgcggcg gctattgacc ccaatcgtat tgtggcgctg
    13681 gagtggttgc cggtggaatt actgctggcg ctcggcatcg tgccttacgg cgtggcggat
    13741 accatcaact atcgcctgtg ggtcagcgaa ccaccattgc cggactcagt gattgacgtc
    13801 ggtttgcgta cagaacctaa ccttgaactg ctgaccgaaa tgaaaccgtc gtttatggtc
    13861 tggtcggcag gatatggccc ttcatcagaa atgctggcac gtattgcgcc gggtcgcgga
    13921 tttaacttca gtgacggcaa acatccgttg gcgatggcgc gtaaatcgct gacggaaatg
    13981 gcagatttac ttaacctgca aagcgcagcg gaaacgcatt tagcgcaata cgaagacttt
    14041 atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac gcccgttact gctgacgacg
    14101 cttatcgatc cgcgccatat gctggtcttc ggtccaaaca gcttgttcca ggaaattctc
    14161 gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca acttctgggg cagtaccgcc
    14221 gtcagtatcg atcgtctggc agcgtataaa gacgttgatg tgctctgttt tgatcacgac
    14281 aacagcaaag acatggatgc gctaatggca accccgctgt ggcaggccat gccgtttgtc
    14341 cgtacagggc gctttcagcg cgtacccgca gtctggtttt atggtgcaac gctttcggca
    14401 atgcactttg tgcgtgttct ggataacgcc atcggaggta aagcgtgagt aaacgaattg
    14461 cgcttttccc ggcgttattg ctggcgctgt tagtgattgt cgctacggcg ctcacctgga
    14521 tgaacttctc acaggcgctg ccgcgcagtc agtgggcgca ggccgcctgg tcgccggata
    14581 ttgacgtcat cgagcagatg atttttcact acagcttgtt gccgcgtctg gcgatttcgc
    14641 tgctggtggg cgcgggcctg gggctggtgg gcgtgctgtt tcagcaagta ctgcgtaacc
    14701 cgctggcgga accgacgacg ttgggcgttg caacaggcgc gcaactgggg attaccgtca
    14761 ccacgctctg ggcgatccct agcgcgatgg cgagccagtt tgctgcgctg gcaggggctt
    14821 gcgttgttgg cttaattgtc tttggcgtcg cgtgggggaa acggctgtcg ccggtaacgc
    14881 tgattttcgc ggggttggta gtgagccttt attgcggcgc aatcaatcag ttactggtta
    14941 tcttccatca tgaccagttg caaagcatgt ttctgtggag cactggaacg ctgacgcaaa
    15001 ccgactgggg cggtgttgag cgtttatggc cgcagttgct gggcggtgtg atgctgacgt
    15061 tgctgctact tcgtccgtta accctgatgg ggcttgatga tggcgtggcg cgcaatctcg
    15121 ggctggcctt gtcgcttgcg cgtctggcgg cgctgtcgct ggcgattgtc atcagtgcgc
    15181 tgctggtgaa cgctgtgggg attatcggct ttatcgggtt gttcgcgccg ctgctggcaa
    15241 aaatgctggg ggcgcggcgt ctgctgccac gactgatgct ggcgtcgttg attggtgcgc
    15301 tgatcctctg gctttccgat caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt
    15361 ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct actgtggctg ctgccgcgtt
    15421 tacgcagcat tagcgcgccg gatatgaagg ttaacgatcg tgtcgcgact gaacgccaac
    15481 atgtgctggc gtttgccctc gcgggcggcg tgctgttgtt gatggctgtg gtggtggcgc
    15541 tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag cggggcgttg ctcgaggatt
    15601 taatgccctg gcgctggccg cgaattatgg cggcgctgtt tgcgggcgtc atgctggcgg
    15661 tagcgggctg tattattcag cgactgaccg gcaacccgat ggcaagcccg gaagtgctgg
    15721 ggattagctc cggcgcggcg tttggtgtgg tgttaatgct gtttctggta ccgggtaatg
    15781 cctttggctg gttgttaccc gcaggcagtc tcggggcggc ggtgacgctg ttgatcatta
    15841 tgatcgccgc cggacgcggt ggattttccc cacaccgcat gttactggcg gggatggcgt
    15901 taagtaccgc gttcaccatg cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg
    15961 cgcaagtgct gacctggatt tccggttcga cctacaacgc gaccgatgct caggtctggc
    16021 gcaccggaat tgtgatggtg attttgctgg cgattactcc gctgtgccgc cgctggctga
    16081 ccattttacc gctgggtggt gataccgcgc gggcggtagg gatggcgctg acgccgacgc
    16141 gaattgcgct gctgctgtta gcggcttgcc tgacggcgac cgcgacgatg actattggac
    16201 cgttgagttt tgttggttta atggcaccgc atattgcgcg gatgatgggc tttcgacgga
    16261 cgatgccaca catcgtaatt tcggcgctgg tgggtggttt actgctggtg ttcgctgact
    16321 ggtgtgggcg gatggtgctg tttccattcc agatcccggc ggggctgctg tcgaccttta
    16381 tcggtgcgcc atattttatc tatttgctga gaaagcagag ccgttaatag aatgcctcgc
    16441 ccggtgggcg aggcaaaatt atcacaactt cgcgaacacc cgacgtgcag catcgatggt
    16501 gttattgata tcttccatgc tgtgcgccac agacataaag cccgcttcaa acgctgacgg
    16561 tgccaggtaa acaccttcat ccagcatcat atggaagaaa cgcttaaaac gctcgacgtc
    16621 acaggccatc acatcctgat agcacgtcac ggactcggcg tcggtaaaga agataccgaa
    16681 cataccaccg acgtggttaa cgaccagcgg aattcccgct tcttctgccg cttccagcag
    16741 accttctgcc agacgtgttg tcagctcatc cagcgtttcg tgaatgcccg gctgcgcgac
    16801 ttcattcaga caggcgaaac ccgctgccat cgcaatcggg ttaccggaaa gcgtacccgc
    16861 ctgatagacc ggacctgtcg gggccagcgc atccattaca tcacgacgac caccgaatgc
    16921 gcctaccggc attccaccgc cgatgatttt tccgaggcag gttaaatctg gcactacggc
    16981 gaaataatcc tgtgcgccag ctagcgctac gcggaaaccg gtcatcactt catcgatgat
    17041 cagcaacgcg ccaaattcgt cgcacagcgc acgcagacct ggcaggaact ctggcagcgg
    17101 cggaacacag ttcatattgc ctgccaccgg ctcgacgata atacaggcaa tctcttgcgg
    17161 gtattgctca aacgcggcgc gtacagaggc cagatcgtta taagtacagg ttaaggtatg
    17221 tttggcgaaa tctgccggaa cgcccggcga gtttggctgg cctaacgtga gtgcgccaga
    17281 accggctttc accagcaggc agtcagcgtg accgtggtaa caaccttcaa atttaatgat
    17341 tttgtcgcga ccggtaaaac cacgggccag gcggatggcg ctcatcgtcg cctcggtgcc
    17401 ggagttcacc atgcgcacca tatccatggt cgggaccagt tcggtcacca gttgcgccat
    17461 tttcacttcc atttcggtcg gtgcaccaaa gcttaaacca cgctcggcgg cttcaatcac
    17521 ggcattgcgg atcgccggat ggttatggcc cagcaccatc ggcccccagg aaccgacata
    17581 atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt tttcgataaa
    17641 cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacac cgccagggat
    17701 cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt cctgattcgt
    17761 agaaaaagtg aatggctgct attccatgtt attcataaca agttaaatac tcgtcaaaca
    17821 tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt tgtgcatttg
    17881 aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc gtcatcttat
    17941 ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta gaaacaccgc
    18001 aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc gataaaaccc
    18061 cgttagccat tttgtttatg gcggcagtcg tcggcacgct tgtcgggctg gctgcggttg
    18121 cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcgctg gtacatactg
    18181 ctgataatta tccgctactg ttaaccgtcg cttttctctg ttcggcggtg ctggcgatgt
    18241 tcggctactt tttggtgcgc aaatacgcgc ccgaagcagg gggatcgggg atcccggaaa
    18301 ttgaaggggc gctggaagat caacgtcctg ttcgctggtg gcgtgtattg ccggtgaaat
    18361 tctttggcgg gctggggaca ctcggcggag gcatggggct agggcgcgaa ggaccaaccg
    18421 tgcagatcgg cggcaatatc agtcgcatgg tgcttgatat tttccgcctg aaaggtgatg
    18481 aagctcgcca tacgctgctg gcaaccggtg ctgctgcggg gctggctgcg gcctttaacg
    18541 cgccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt cgctatacgt
    18601 taatttcgat taaagcggta tttattggcg tcattatgtc gaccattatg taccggattt
    18661 ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgatgcgccg cttaatacgc
    18721 tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt tttaataaat
    18781 gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg cggcaatatt accaaatggg
    18841 tactgatggg gggtgcgatt ggcggtctgt gtggattgct ggggtttgtg gcaccagcaa
    18901 cgtcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc agcatgggaa
    18961 tgctggtgtt tatcttcgtc gctcgggtca ttaccacctt actctgcttc tcttccggcg
    19021 cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga accgctttcg
    19081 gaatggttgc cgttgagctg tttccgcaat atcacctgga ggcggggacg tttgctattg
    19141 ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc gttaacgggg atcattctgg
    19201 ttctggagat gaccgataac taccagctca ttttgccaat gattattacc ggtcttggcg
    19261 caacactatt agcgcaattt accggcggga aacctctata ctcggcgatt cttgcgcgca
    19321 cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa ggccgcatca gccagcgaga
    19381 atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt tagaatttgc
    19441 ccgattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg ccgctggagt
    19501 ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat aacccgaatc
    19561 tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat ggtttcacct
    19621 ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt ggcctggtgg
    19681 ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactacacc gaaggactgg
    19741 aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt tgcggttctt
    19801 cctttagcat ctaatctgtt gttcatgtgc cggataaact atccggcaca tcttctctct
    19861 tcaacgccca ttctcatcca gcgcaaacgt cggtagctta agatgccaac gaatagccgc
    19921 cagccgaatc aatagcgtca cgaccatgcc catcatactg gctgtttcca gtggtacgga
    19981 aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggcag ttgcgtagat
    20041 ttctgtacgt aaaatcatgg ggatttcgcg gaccagtaca tcacgaatga tcccgccgcc
    20101 aacgccagta atgacgccca tacaaaccgc gattaacgga ccggcttccg cattaaaggc
    20161 tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca ccggcaacat
    20221 ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc tggtgaccat
    20281 ggcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat ccagcgccat
    20341 gtcgcgaatt gttccgtcgc ctactgcggt aaccacgccc agtaccagaa caccaaaagg
    20401 gtccatacgc aattttccgg ctaacaaaac gccgtagatg gcaaataccg ctgtgccgac
    20461 tatatccagc caatagacga gcattgttca atccccactg agcacctgaa aaggtcaggc
    20521 actaatctac ctgtgaaagc gcattacaga gctgttgtgc tgcgaggata atacgtgggc
    20581 ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc tgttcacccc
    20641 agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaata gcctgtggcg
    20701 agcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg tctttaaaga
    20761 tgttttctcc gccacaaact tcgagtacct ggttctgaat cgactctttt ccactggtaa
    20821 atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca gcatattgcg
    20881 ctttcaattg cacgtactga tccagcaggg attgcgcggc ttgttcggcc ttgtctggtt
    20941 gcggactcca gggggccagt tgacgtaacg cattggcaat ttgttcaatg cttgtcgcat
    21001 cgacccacat cacttttatt cccagcgaag ccagctggtc aacctgccgc tcggcattac
    21061 ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac aatgcgttcc agattcatcc
    21121 cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg aggatagtcg gaatagctgc
    21181 tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg ggagaaagcg
    21241 tgatgacgcg cggcgcggcg ttgagccaca gtggcgcgag aaaagacagg gcgaccagcg
    21301 ccctgaacag tgatttagcc atgtgccagt ttctgcacca gtgactcaac catcaggctg
    21361 gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg ttgatcggcc
    21421 acgtcggaga tggcgcgtac gacaacaaac gggacgttga aattgtggca gacatgggcg
    21481 attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg gattttcgcc
    21541 agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa tcaggccacg tacagcgtta
    21601 agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc agctttaaag
    21661 cctgccggac agcctggtaa ctgaccgtat tcataaccaa atgccgtgac atccgcgtcg
    21721 tgataacgcg cttcgtccga gacaacgata tcgcccactt tcagcgttgg tgccaggcca
    21781 ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag cagagtggca
    21841 cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc ggttccattc
    21901 agttggccgg tatagatttc gcaaccgccg agactgatag tttgacggtt ttcgattttg
    21961 tcacgcagca gcgtaacttc ttcttccatt gcaccaatga tgccgatttt catagattta
    22021 ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg cttacgggga agcgtatttc
    22081 tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact ggcatcgtcg
    22141 ttaccgttca ccgcagggcg ttaaaaccga acatgagatc ctgcggatct tcgagagcga
    22201 tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaga cccaggtttt
    22261 tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg aagtccagca
    22321 ggtgggacgc tacatcgcca aagaaatttt aagccgtctg aaagagctta aattgctgga
    22381 agcatacggc ctggatgaac tgaccggccc ctttgaaagc attgttgaga tgtcatgcct
    22441 gatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcagcga taaatgactg
    22501 gtttcgccaa cgtttgcacc cggaagatgc cgaaagccag cctctgactg acgatcgctg
    22561 cagcgtggcg gcactacgtt tacgggacgg ggaagaaccg cttaacgagc tgcggcgcaa
    22621 gattcgtcag gacttatgtc attttgaggg gaatgcacaa ggcattcgtc tggtgcatac
    22681 attgatgcgg atgaatctca cttgggcaca ggttggcggt attttaaaat atacccgtcc
    22741 ggcgtggtgg cgtggcgaaa cgcctgagac acatcactat ttaatgaaaa agccgggtta
    22801 ttatctttct gaagaagcct atattgcccg cttgcgtaaa gaacttaatt tggcgcttta
    22861 cagtcgtttt ccattaacgt ggattatgga agctgccgac gacatctcct attgtgtggc
    22921 agaccttgaa gatgcggtag agaaaagaat atttaccgtt gagcagcttt atcatcattt
    22981 gcacgaagcg tggggccagc atgagaaagg ttcgctcttt tcgctggtgg ttgaaaatgc
    23041 ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg gaagatcagt tttttatgta
    23101 tttacgggta aacaccctaa ataaactggt accctacgcg gcacaacgat ttattgataa
    23161 tctgcctgcg attttcgccg gaacgtttaa tcatgcatta ttggaagatg ccagcgaatg
    23221 cagcgatctt cttaagctat ataaaaatgt cgctgtaaaa catgtgttta gccatccgga
    23281 tgtcgagcag cttgaattgc agggctatcg ggtcattagc ggattattag agatttatcg
    23341 tcctttatta agcctgtcgt tatcagactt tactgaactg gtagaaaaag aacgggtgaa
    23401 acgtttccct attgagtcgc gcttattcca caaactctcg acgcgccatc ggctggccta
    23461 tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag tttccgctat gggaatatta
    23521 ttaccgttgc cgcctgctgc aggattatat cagcggtatg accgacctct atgcgtggga
    23581 tgaataccga cgtctgatgg ccgtagaaca ataaccaggc ttttgtaaag acgaacaata
    23641 aatttttacc ttttgcagaa actttagttc ggaacttcgg gctataaaac gaatctgaag
    23701 aacacagcaa ttttgcgtta tctgttaatc gagactgaaa tacatgaaaa aaaccacatt
    23761 agcactgagt gcactggctc tgagtttagg tttggcgtta tctccgctct ctgcaacggc
    23821 ggctgagact tcttcagcaa cgacagccca gcagatgcca agccttgcac cgatgctcga
    23881 aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt agcacaaccg ttaatacgcc
    23941 gcgtatgccg cgtaatttcc agcagttctt cggtgatgat tctccgttct gccaggaagg
    24001 ttctccgttc cagagctctc cgttctgcca ggggggccag ggcggtaatg gcggcggcca
    24061 gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt gatgccgata aaggctatgt
    24121 cgtcaccaac aaccacgttg ttgataacgc gacggtgatt aaagtccagc tgagcgatgg
    24181 tcgtaagttc gacgcgaaga tggttggcaa agatccgcgc tctgatatcg cgctgatcca
    24241 aatccagaac ccgaaaaaac tgaccgcaat taagatggcg gattctgatg cactgcgcgt
    24301 gggtgattac accgtagcga ttggtaaccc gtttggtctg ggcgagacgg taacttccgg
    24361 gattgtctct gcgctggggc gtagcggcct gaatgctgaa aactacgaaa acttcatcca
    24421 gaccgatgca gcgatcaacc gtggtaactc cggtggtgcg ctggttaacc tgaacggcga
    24481 actgatcggt atcaacaccg cgatcctcgc accggacggc ggcaacatcg gtatcggttt
    24541 tgctatcccg agtaacatgg tgaaaaacct gacctcgcag atggtggaat acggccaggt
    24601 gaaacgcggt gagctgggta ttatggggac tgagctgaat tccgaactgg cgaaagcgat
    24661 gaaagttgac gcccagcgcg gtgctttcgt aagccaggtt ctgcctaatt cctccgctgc
    24721 aaaagcgggc attaaagcgg gtgatgtgat cacctcactg aacggtaagc cgatcagcag
    24781 ctttgccgca ctgcgtgctc aggtgggtac tatgccggtg ggcagcaaac tgaccctggg
    24841 cttactgcgc gacggtaagc aggttaacgt gaacctggaa ctgcagcaga gcagccagaa
    24901 tcaggttgat tccagctcca tcttcaacgg cattgaaggc gctgagatga gcaacaaagg
    24961 caaagatcag ggcgtggtag tgaacaacgt gaagacgggc actccggctg cgcagatcgg
    25021 cctgaagaaa ggtgatgtga ttattggtgc gaaccagcag gcagtgaaaa acatcgctga
    25081 actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca ctgaacattc agcgcggcga
    25141 cagcaccatc tacctgttaa tgcagtaatc tccctcaacc ccttcctgaa tacgggaagg
    25201 ggttctcctt acaatctgtg aacttcgcca caactccata catcttcatc atcctttggg
    25261 catttgcaca atgccgtacg ttacgtactt ccttatgcta agccgtgcat aacggaggac
    25321 ttatggctgg ctggcatctt gataccaaaa tggcgcagga tatcgtggca cgtaccatgc
    25381 gcatcatcga taccaatatc aacgtaatgg atgcccgcgg gcgaattatc ggcagcggcg
    25441 atcgtgagcg tattggtgaa ttgcacgaag gtgcattgct ggtactttca cagggacgag
    25501 tcgtcgatat cgatgacgcg gtagcacgtc atctgcacgg tgtgcggcag gggattaatc
    25561 taccgttacg gctggaaggt gaaattgtcg gcgtaattgg cctgacaggt gaaccagaga
    25621 atctgcgtaa atatggcgaa ctggtctgca tgacggctga aatgatgctg gaacagtcgc
    25681 ggttgatgca cttgttggcg caggatagcc gtttgcggga agaactggtg atgaacctga
    25741 ttcaggcaga ggagaatact cccgcacttc tgaatgggcg caacggctgg ggatcgatct
    25801 caatcaaccg cgagtggtgg ctattgttga ggtcgacagc ggtcagcttg gcgtggacag
    25861 cgcaatggcg gagttacaac aactgcaaaa cgcgctgact acgcccgagc gtaataatct
    25921 ggtggcgatt gtctcgctaa ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg
    25981 gcgctgggat gcagaagatc atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga
    26041 gtacggccag ctgcgttttc gcgtttcact gggcaactat tttaccggtc ctggcagtat
    26101 tgcccgatcc tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga
    26161 aagtcgctgc tatttttatc aggatctgat gttacctgtg ttactcgaca gtttgcgtgg
    26221 cgactggcag gccaacgaac tggcgcgacc gctggcgcgg ctgaaagcga tggacaataa
    26281 cggcttgctg cgacgaacgc tggcggcgtg gtttcgccac aatgtgcaac cgctggcaac
    26341 gtcaaaggcg ttgtttattc atcgtaatac cctggagtat cggcttaatc gtatatcgga
    26401 actgaccggg cttgatttgg gcaattttga tgacaggttg ctgctgtatg tggcgttaca
    26461 actggatgaa gagcggtaga ttatgcgtta aggttgaggc agcgctggct cattcccctc
    26521 agcccgaagg ggagagggga atgcccgagt taaccgtcaa ttcgggatta tttacgcgtt
    26581 agtttttcca gatcggcttc aatctcgctg atcttattgg ttacgactga ctccaggtga
    26641 cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc tgcggtcacg ctgacagatt
    26701 tgatcaagct catcaatgat ataccgtaga ttcgggctga tttcctggac ttctttataa
    26761 ccctgaccca caccatcagc gacgaccgtt ttacgctgac gcggatattt aaacttaacg
    26821 ctcttggcga aaaactcgcc tttgtctttc tggaaataga ttttcagaat atcgttgttg
    26881 gcttcctgcc ggaggctgta acgatcaatt tcttcaggat tggtaatacc cagacttttc
    26941 agattgtcgt acatagcgtt acctcaaaat gagtcagtaa attgtgctta ttttagcatt
    27001 tggcttggcc cgcccggctc gactgtgatc gcagaaagcc tttttcttcc agacttctat
    27061 catggcgtaa aaatcaaaaa attacctgct ttattgtggc gatattccat gggatggatt
    27121 agcacattta tcttcgtttt gcgaattaca tgacagtccg aaacaagatg aaaaagtatt
    27181 taaaaaaggg ggaggaagaa tgagtaggac gaattattct gagccagaaa gcatacgggc
    27241 agcatcatgc tgcccgtata tgtactctta atcgatggta cgcagcagtt cgttaatgcc
    27301 gactttgccg cgagttttcg cgtcaacttt cttaacgata accgcacagt agaggctgta
    27361 tttgccatct tttgacggca gattacctga aacaaccaca gaccccgccg gaacgcgacc
    27421 gtagtggatt tcgccggttt cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc
    27481 catagagatg acggaacctt cttcgacaat caccccttca accacttcag agcgtgcgcc
    27541 aatgaagcag ttatcttcaa taatggtcgg gttagcctgc agcggctcca gcacgccgcc
    27601 gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc tgcgcacaag aaccgacggt
    27661 cgcccaggta tcaaccatgg tgccttcatc aacatatgcg ccgatgttaa cgtaagacgg
    27721 catcagcacg gtgttacggg caataaacgc accctgacgt accgccgctg gtggcacaac
    27781 gcggaagcct tctttctgga aacgtgcttc gtcgtagtcg gcgaatttca tcggcacttt
    27841 gtcgaagtag cggctttctg ccccttcgat cacctgatta tcattaatac ggaaagagag
    27901 cagcaccgct tttttcaacc actgatgcgt cacccactga ccgtcaattt tttccgctac
    27961 acgcagtgcg ccggaatcca gcagggcgat cacctgattt accgcttcgc gggtaacggt
    28021 gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg
    28081 taactgctgc attgttaaac tcttttcata tcagtaaaca catcaccctt tatcgtttgg
    28141 attgagggcc tctgtcaacc gctgatgcac ttcctgctgc aactcgttat taagcgcacg
    28201 ccggtcagcg gtggcaatta tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg
    28261 ggcaccatga agcgaaattc ccagatcggc aaaaattttc ccgactcgcg ccagcagtcc
    28321 aggttggtcg agggcgatca gttcgaggaa cgatttgcgg tcggtatggg tcggcaaaaa
    28381 cgttacttcg gtttcaacag taaaatggcg taatttggcg ggttggcgac ggggctgcgg
    28441 tggctgccag ctactttgcg tcagcacctg ctccagacca aaccgaataa cctcatggcg
    28501 atctgcggac agcgggctgc catcgggttc cagcacgata aaggtatcca tcgccatacc
    28561 gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc
    28621 acagacggcg gcaaacagat aggggcggtc cgggctccag ataaaaatct cggtgcctcc
    28681 acgcgtagcc tgcgggctaa gcaataccag cggtttgctt aaatcatgct gtaataaatg
    28741 gcgggcatgc caggccagtt gatttgggct atggcggaca aaatagttag cacgacagcg
    28801 tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc atgcgcagta gtgccagtgc
    28861 ctggagttgg tgatggcgaa cccgttcgcg catatccggc gtgttttgca ttccgcgtcg
    28921 tagctgcttt tcggtggcaa aatagagttc gcgcaacaga ctttgcttcc agctattcca
    28981 cagtgtttcg ttggtggcgc aaatgtcagc cactgtcagg cataccagat agcgcagacg
    29041 attttccgtt tgaacttctt cggcaaactg cttgatgact tcagggtcct gaatatcgcg
    29101 gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc agccaggcaa ccagctgtgt
    29161 ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact acatcctgag caccgagaat
    29221 ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag
    29281 ctcagttgac ggcaggcgcg gccagacgtc cacacacaac ggatggcgct ggcgcgtttc
    29341 ttcactggca aaactctcca gtttcagcat cacgcggata gtatgttcat ccaccgtgta
    29401 ggcgtggaac agatcaaact gcatctgccc gacgatatgc gaccattgcg gcatatacgc
    29461 gccgagcacg ctatggcgat gcattggcaa tagtccgcgc cgcaccgctc cggggtgacg
    29521 cagaatgctc aaaaacagtt ttcgtgcttg cggaatatta cacagcggtt gttgcagatg
    29581 gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa atgccggtga tcgcactgtt
    29641 gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc tggcgcataa atagtgtttc
    29701 atcacgcagg tcgattagcg taccgcgtag ctgaaactca tcgtcgagtg gacgtggttt
    29761 ttcgtcggcg ggaagggcga ggatggcttc atcgaacagt tgcagcagca tctggttgag
    29821 ttcactgacg cggcgtgtaa cgcggaagta atccttcatc atccgctcga ccggttcgtt
    29881 gccttcacca ctgtaattca gacgctgagc gacgctaagt tggcgatcga ataacagacg
    29941 attatcgtaa cggctgacga ccagatgcag ggcaaagcga atacgccaca gtatatgcag
    30001 acattcgttt aattccgccc gctccgctga ggttaagaag ccaaacccga ccatttcatc
    30061 cagcgatgtt gcgccaaaat gacggcgggc cacccattgc agagtgtgaa tatcgcgcaa
    30121 gccgccaggg ctacttttga tgtctggttc aaggttgtag ctggtgccat ggtaacgctg
    30181 atggcgctgg ttctgttctt caactttcgc tgcgtagaac ttgtcggaag gccagaatcc
    30241 ttcgctaaaa atatgttttt gcagttcgag gaagagcgca acatcgccaa ttaataagcg
    30301 ggattcgatt aaattggtgg cgacggttaa atccgataac ccttccaaca tgcactcttc
    30361 aagcgtgcgc acactatgac cgacttccag ctttacatcc cagagcagcg ttaacagctc
    30421 gcccactttt tgcgcctgat cgtccgggag ctttttacgg cttaaaatca gtaaatcgat
    30481 gtcagaaagt ggatgcagct cgccacgacc gtagccaccg acggcaacca atgccaggtc
    30541 ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc aggagctggt ctatgaactc
    30601 ggtacgtgcc tcaatcaact gttctgcaga gatcccattg tcaaaggcat cacccagcca
    30661 acgctggaaa gtatcgatat gggcttttat cccacagacg gttaattcat cacggggcca
    30721 ggcgcatgga ttttgcggtt gaccgggcag ggtggagaga gcggtgtttg cgtactgttc
    30781 tggaagggta ttcattgtgc gccacccata agattaaatt atcgcattaa aaaagccggc
    30841 attcgccggc ttcatcttat tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc
    30901 gtagcgtcaa aatttcgcag ccgttatcag tcaccacaat agtatgctca tattgtgcag
    30961 acaagctgcg atctttggtt tttaccgtcc agccatcttt catggtgcgg atctcttttt
    31021 tacccgcgtt gaccattggc tcaatggtga acgtcatccc cggtttcagc acgacgttgg
    31081 tttcacggga gtcatagtgc agcacctgcg gttcttcatg gaagccgcga ccaataccgt
    31141 gtccgcaata ttcacgaacg acggagaagc cttctgcttc gacaaatttc tgaatcgccg
    31201 caccgatttc gcgcagatta atgcctggtt ttaccatgcg tagcgccagg tacaggcttt
    31261 cttgcgtgat gcggcacagg cgttcgccca tgatggtcgg cttaccgacg ataaacattt
    31321 tcgaggtatc gccgtggaaa ccatctttga ttacggtgac atcaatgtta acgatatcgc
    31381 catctttcag cagcttagca tcgtccggga taccgtggca caccacttca ttaatagaga
    31441 tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat
    31501 taacaatgta atcattacag atgcgatcca gctcgccggt gctgacgccc ggtttaacat
    31561 acggttcgat catctccagc acttcggcag ccagtcggcc agcgacgcgc attttttcga
    31621 tatcttctgg ggtcttgatt gagatagcca ttaattctgt ccatcagcgt cggtgatacc
    31681 gacaatatat atgtaagtgc cgtcaatggt atcacacccg ggcaaattga gaatcattct
    31741 gaatttcgcc aaacgtgcca ctgaaggttt tctataatag aaaattcgac gtctgatgct
    31801 gtacacagcg ccaacaatta ttggtgtcca cgacgtattt gtggtataaa gcgcgccgga
    31861 cttccgatcc atttcgtata cacagactgg acggaagcga caatctcact ttgtgtaaca
    31921 acacacacgt atcggcacat attccggggt gccctttggg gtcggtaata tgggatacgt
    31981 ggaggcataa ccccaacttt tatatagagg ttttaatcat ggcaactgtt tccatgcgcg
    32041 acatgctcaa ggctggtgtt cacttcggtc accagacccg ttactggaac ccgaaaatga
    32101 agccgttcat cttcggtgcg cgtaacaaag ttcacatcat caaccttgag aaaactgtac
    32161 cgatgttcaa cgaagctctg gctgaactga acaagattgt ttctcgcaaa ggtaaaatcc
    32221 ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg
    32281 accagttctt cgtgaaccat cgctggctgg gcggtatgct gactaactgg aaaaccgttc
    32341 gtcagtccat caaacgtctg aaagacctgg aaactcagtc tcaggacggt actttcgaca
    32401 agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc
    32461 tgggcggtat caaagacatg ggcggtctgc cggacgctct gtttgtaatc gatgctgacc
    32521 acgaacacat tgctatcaaa gaagcaaaca acttgggtat tccggtattt gctatcgttg
    32581 ataccaactc tgatccggac ggtgttgact tcgttatccc gggtaacgac gacgcaatcc
    32641 gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg
    32701 atctggcttc ccaggcggaa gaaagcttcg tagaagctga gtaataaggc ttgataactc
    32761 ccccaaaata gttcgagttg cagaaaggcg gcaagctcga gaattcccgg gagcttacat
    32821 cagtaagtga ccgggatgag cgagcgaaga caacgcatct gcggcgcgaa atatgaaggg
    32881 ggagagccct tatagaccag gtagtacacg tttggttagg gggcctgcat atggccccct
    32941 ttttcacttt tatatctgtg tggtttaatg ccgggcagat cacatctccg aggattttag
    33001 aatggctgaa attaccgcat ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat
    33061 gatggattgc aaaaaagcac tgactgaagc taacggcgac atcgagctgg caatcgaaaa
    33121 catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg
    33181 cgtgatcaaa accaaaatcg acggcaacta cggcatcatt ctggaagtta actgccagac
    33241 tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca gacaaagttc tggacgcagc
    33301 tgttgctggc aaaatcactg acgttgaagt tctgaaagca cagttcgaag aagaacgtgt
    33361 tgcgctggta gcgaaaattg gtgaaaacat caacattcgc cgcgttgctg cgctggaagg
    33421 cgacgttctg ggttcttatc agcacggtgc gcgtatcggc gttctggttg ctgctaaagg
    33481 cgctgacgaa gagctggtta aacacatcgc tatgcacgtt gctgcaagca agccagaatt
    33541 catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa gaataccagg tacagctgga
    33601 tatcgcgatg cagtctggta agccgaaaga aatcgcagag aaaatggttg aaggccgcat
    33661 gaagaaattc accggcgaag tttctctgac cggtcagccg ttcgttatgg aaccaagcaa
    33721 aactgttggt cagctgctga aagagcataa cgctgaagtg actggcttca tccgtttcga
    33781 agtgggtgaa ggcatcgaga aagttgagac tgactttgca gcagaagttg ctgcgatgtc
    33841 caagcagtct taattatcaa aaaggagccg cctgagggcg gcttcttttt gtgcccatct
    33901 tgtaaattca gctaaccctt gtggggctgc gctgaaaagc gacgtacaat gtcgctagta
    33961 ttaattcatt tcaatcgttg acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc
    34021 gtctataaac gcattctgct taagttgagt ggcgaagctc tgcagggcac tgaaggcttc
    34081 ggtattgatg caagcatact ggatcgtatg gctcaggaaa tcaaagaact ggttgaactg
    34141 ggtattcagg ttggtgtggt gattggtggg ggtaacctgt tccgtggcgc tggtctggcg
    34201 aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga tgctggcgac cgtaatgaac
    34261 ggcttggcaa tgcgtgatgc actgcaccgc gcctatgtga acgctcgtct gatgtccgct
    34321 attccattga atggcgtgtg cgatagctac agctgggcag aagctatcag cctgttgcgc
    34381 aacaaccgtg tggtgatcct ctccgccggt acaggtaacc cgttctttac caccgactca
    34441 gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt
    34501 gacggcgtgt ttaccgctga tccggcgaaa gattcaaccg caaccatgta cgagcaactg
    34561 acttacagcg aagtgctgga aaaagagctg aaagtcatgg acctggcggc cttcacgctg
    34621 gctcgtgacc ataaattacc gattcgtgtt ttcaatatga acaaaccggg tgcgctgcgc
    34681 cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg aataattccc gtgatggata
    34741 aataagggta agattccgcg taagtatcgc gggggcgtaa gtctggttat aaggcgttat
    34801 tgttgcaggc agtttggtca cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga
    34861 ggatggcgag cactgctcgg ggccaaaatg gcaaataaaa tagcctaata atccagacga
    34921 ttacccgtaa tatgtttaat cagggctata cttagcacac ttccactgtg tgtgactgtc
    34981 tggtctgact gagacaagtt ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat
    35041 gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc
    35101 acgggtcgtg cttctcccag cctgctggat ggcattgtcg tggaatatta cggcacgccg
    35161 acgccgctgc gtcagctggc aagcgtaacg gtagaagatt cccgtactct gaaaatcaac
    35221 gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga ttatggcctc cgatctcggc
    35281 ctgaacccga actctgcggg tagcgacatc cgtgttccgc tgccgccgct gacggaagaa
    35341 cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta
    35401 cgtaatgtgc gtcgtgacgc gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc
    35461 agcgaagacg acgatcgccg ttctcaggac gatgtacaga aactgactga tgctgcaatc
    35521 aagaaaattg aagcggcgct ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt
    35581 gaacgacaaa aacgccgctc agtagatcct tgcggatcgg ctggcggcgt tttgcttttt
    35641 attctgtctc aactctggat gtttcatgaa gcaactcacc attctgggct cgaccggctc
    35701 gattggttgc agcacgctgg acgtggtgcg ccataatccc gaacacttcc gcgtagttgc
    35761 gctggtggca ggcaaaaatg tcactcgcat ggtagaacag tgcctggaat tctctccccg
    35821 ctatgccgta atggacgatg aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca
    35881 ggggagccgc accgaagtct taagtgggca acaagccgct tgcgatatgg cagcgcttga
    35941 ggaggttgat caggtgatgg cagccattgt tggtgctgct gggctgttac ctacgcttgc
    36001 tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa gaatcactgg ttacctgcgg
    36061 acgtctgttt atggacgccg taaagcagag caaagcgcaa ttgttaccgg tcgatagcga
    36121 acataacgct atttttcaga gtttaccgca acctatccag cataatctgg gatacgctga
    36181 ccttgagcaa aatggcgtgg tgtccatttt acttaccggg tctggtggcc ctttccgtga
    36241 gacgccattg cgcgatttgg caacaatgac gcccgatcaa gcttgccgtc atccgaactg
    36301 gtcgatggga cgtaaaattt ctgtcgattc ggctaccatg atgaacaaag gtctggaata
    36361 cattgaagcg cgttggctgt ttaacgccag cgccagccag atggaagtgc tgattcaccc
    36421 gcagtcagtg attcactcaa tggtgcgcta tcaggacggc agtgttctgg cgcagctggg
    36481 ggaaccggat atgcgtacgc caattgccca caccatggca tggccgaatc gcgtgaactc
    36541 tggcgtgaag ccgctcgatt tttgcaaact aagtgcgttg acatttgccg caccggatta
    36601 tgagcgttat ccatgcctga aactggcgat ggaggcgttc gaacaaggcc aggcagcgac
    36661 gacagcattg aatgccgcaa acgaaatcac cgttgctgct tttcttgcgc aacaaatccg
    36721 ctttacggat atcgctgcgt tgaatttatc cgtactggaa aaaatggata tgcgcgaacc
    36781 acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg agtgaagtcg ccagaaaaga
    36841 ggtgatgcgt ctcgcaagct gaggataatc cggctacaga gagtcgcgct atttgttagc
    36901 gtagggcttc agtgatatag tctgcgccat ctgatcgtaa gtagttggct ttataaggtc
    36961 aggtatgccg tggttttaca cggctttttt ttgtataggc ttcagtattc ctgagtaccg
    37021 taaaccctgt cagggaataa aaaacgcgtg atgttgtctg ctactcaacc acttagcgaa
    37081 aaattgccag cgcatggctg ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg
    37141 gcaaaaaagc aagggaagat tcgtgccttt gggcataaag ccggggcaaa atccgtccgc
    37201 cgggctgtct cttttgcggc caacaacggt attgaggcgt taacgctgta tgcctttagt
    37261 agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg
    37321 gcgctcgata gcgaagtaaa aagtctgcac cgacataacg tgcgtctgcg tattattggc
    37381 gataccagtc gttttaactc gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca
    37441 gccggaaata ccggtctgac gctgaatatt gcggcgaact acggtggacg ttgggatata
    37501 gtccagggag tcaggcaact ggctgaaaag gtgcagcaag gaaacctgca accagatcag
    37561 atagatgaag agatgttaaa ccagcatgtc tgtatgcatg aactggcccc tgtagattta
    37621 gtaattagga ctgggggaga gcatcgcatt agtaactttt tgctttggca aattgcctat
    37681 gccgaacttt actttacaga tgttctctgg cccgatttcg atgaacaaga ctttgaaggg
    37741 gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa
    37801 acagcctgat gggggtcgct tttgctgaag tatcgcctga tatctgcttt tgtgttaata
    37861 cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg ggttcgccat tgtaacgctg
    37921 gtggtctgca tgctggcagc gtgggaatgg ggacagctta gcggttttac cactcgttcg
    37981 cagcgagtat ggttggcggt gttatgcggg ttattgttgg cgctgatgct ttttctgttg
    38041 ccggaatatc accgaaatat tcatcaaccg ctggttgaaa tctcactttg ggcttcgttg
    38101 ggttggtgga ttgtcgcgct attgctggtg ctgttttacc ctggttccgc agcaatctgg
    38161 cgtaactcta aaacattgcg ccttattttt ggcgtgctaa ccattgttcc cttcttctgg
    38221 ggcatgctgg cgttacgggc ctggcactat gacgagaatc attacagtgg cgcaatatgg
    38281 ctgctctatg tcatgatcct ggtatggggc gctgactccg gcgcatatat gtttggcaaa
    38341 ttgtttggta aacataagct ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt
    38401 ataggtggac tcgctactgc agcggtaatc tcatggggtt atggcatgtg ggcgaatctc
    38461 gacgtcgctc ccgtcacctt actcatttgc tctattgtcg cagcgttagc ctcagtgctc
    38521 ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa ttaaggacag cggtcattta
    38581 attccaggac acggtggtat tttagatcgt attgatagcc tgacggctgc ggtaccggtc
    38641 tttgcttgct tgttgttact ggtattcagg acgctttaac ggaaggtaat atgctgagtt
    38701 ttctctggga tttggcttcg ttcatcgttg cactgggtgt acttatcacc gtgcatgaat
    38761 ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt
    38821 ttggtaaggc gctctggcgg cgaactgata agctcggcac cgaatatgtt atcgccctga
    38881 tcccgttggg cggttatgtc aaaatgctgg atgagcgcgc agaaccggtc gttccggaac
    38941 tccgccacca tgccttcaat aataaatctg tcggccaacg agcggcgatt attgccgcag
    39001 gtccggttgc aaacttcatt tttgctatct ttgcctactg gctggttttt attattggtg
    39061 tgcctggcgt acgtccggtg gttggcgaaa tagcagccaa ttcgatagct gcggaagcac
    39121 aaattgcacc aggtacggaa ctaaaagccg tagatggtat cgaaacgcct gattgggatg
    39181 ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag caccaccatt acagtagcgc
    39241 catttggcag cgaccaacgg cgggatgtaa agctcgattt acgtcactgg gcgtttgagc
    39301 ctgataaaga agatccggta tcttcgctgg ggattcgtcc tcgtgggccg caaattgaac
    39361 ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg
    39421 acaggatcgt taaagtcgat ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg
    39481 tccgggataa cccgggtaaa tccttagcgt tagaaatcga aaggcagggg agtcccttgt
    39541 ctttgacatt aatcccgggg agtaaaccgg gtaatggtaa agcgattggt tttgtcggta
    39601 ttgagccgaa agtcattcct ttgccagatg agtataaagt tgtacgccag tatgggccgt
    39661 tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct gatgaagctg acggtcagta
    39721 tgctgggaaa attgatcacc ggtgatgtga aactgaacaa cctcagtggg ccgatctcta
    39781 tcgccaaggg ggctgggatg acagcggaac tcggggtagt ttattacctg ccgtttcttg
    39841 cgcttattag cgtgaactta gggataatta acctgtttcc gttgcccgta cttgacgggg
    39901 ggcatctgct gttccttgcg atcgaaaaga tcaagggcgg accggtatcc gagcgggttc
    39961 aagacttttg ttatcgcatt ggctcgattc tgctggtgct gttaatgggg cttgcacttt
    40021 tcaatgattt ctctcggtta tgagagttag ttaggaagaa cgcataataa cgatggcgat
    40081 gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg
    40141 gttcgtagtg aaagatattc atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct
    40201 cctcagtatg ccggtgcgta caggcgacac ggttaatgat gaagatatca gtaataccat
    40261 tcgcgctctg tttgctaccg gcaactttga ggatgttcgc gtccttcgtg atggtgatac
    40321 ccttctggtt caggtaaaag aacgtccgac cattgccagc attactttct ccggtaacaa
    40381 atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga
    40441 atccctcgat cgcaccacca ttgccgatat cgagaaaggt ctggaagact tctactacag
    40501 cgtcggtaaa tatagcgcca gcgttaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt
    40561 tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt
    40621 tggtaaccat gctttcacca ccgacgaact gatctctcat ttccaactgc gtgacgaagt
    40681 gccgtggtgg aatgtggtag gcgatcgtaa ataccagaaa cagaaactgg cgggcgatct
    40741 tgaaaccctg cgcagctact atctggaccg cggttatgcc cgtttcaaca tcgactctac
    40801 ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc acggtgaaca tcaccgaagg
    40861 cgatcagtac aagctttctg gcgttgaagt gagcggcaac cttgccgggc actccgctga
    40921 aattgagcag ctgactaaga tcgagccggg cgagctgtat aacggcacta aagtgaccaa
    40981 aatggaagat gacattaaaa agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca
    41041 gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa ttacgcgtga acgttgatgt
    41101 gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt aacgatacct cgaaagatgc
    41161 cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg ttggggagcg atctggtcga
    41221 tcagggtaag gagcgtctga atcgtctggg cttctttgaa actgtcgata ccgataccca
    41281 acgtgttccg ggtagcccgg accaggttga tgtagtctac aaggtgaaag aacgtaacac
    41341 tggtagcttc aacttcggga ttggttacgg tactgaaagt ggcgtgagct tccaggctgg
    41401 cgtgcaacag gataactggt taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa
    41461 cgattaccag acctatgctg aactgtcggt aaccaacccg tacttcaccg tagatggcgt
    41521 aagcctcggt ggtcgtctct tctataatga cttccaggca gatgacgccg acctgtccga
    41581 ctataccaac aagagttatg gtacagacgt gacgttgggc ttcccgatta acgaatataa
    41641 ctcgctgcgt gcaggtctgg gttatgtaca taactccctg tccaacatgc agcctcaggt
    41701 tgcgatgtgg cgttatctgt actctatggg tgaacatccg agcacctctg atcaggataa
    41761 cagcttcaaa acggacgact tcacgttcaa ctatggttgg acctataaca agcttgaccg
    41821 tggttacttc ccgacagatg gttcacgtgt caacctgacc ggtaaagtga ccattcctgg
    41881 atcggataac gaatactaca aagtgacgtt agacacggcg acttatgtgc cgatcgatga
    41941 cgatcacaaa tgggttgttc tgggtcgtac ccgctggggt tatggtgatg gtttaggcgg
    42001 caaagagatg ccgttctacg agaacttcta tgccggtggt tccagcaccg tgcgtggctt
    42061 ccagtccaat accattggtc cgaaagcagt ttacttcccg catcaggcca gtaattatga
    42121 tccggactat gattacgaat gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga
    42181 tgatgctgta ggcggtaacg ccatggcggt tgccagcctc gagttcatca ccccgacgcc
    42241 gtttattagc gataagtatg ctaactcggt tcgtacttca ttcttctggg atatgggtac
    42301 cgtttgggat acaaactggg attccagcca atattctgga tatccggact atagtgatcc
    42361 aagcaatatc cgtatgtctg cgggtatcgc attacaatgg atgtccccat tggggccgtt
    42421 ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga gacaaggcag aacagttcca
    42481 gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc
    42541 gatgacttta ggcgatcaat ataagatcgc cgggccacgc aaagaactgc accctccggt
    42601 gcaaatggga tggtaaggag tttattgtga aaaagtggtt attagctgca ggtcttggtt
    42661 tagcactggc aacttctgct caggcggctg acaaaattgc aatcgtcaat atgggcagcc
    42721 tgttccagca ggtagcgcag aaaaccggtg tttctaacac gctggaaaat gagttcaaag
    42781 gccgtgccag cgaactgcag cgtatggaaa ccgatctgca ggctaaaatg aaaaaactgc
    42841 agtccatgaa agcgggcagc gatcgcacca aactggaaaa agacgtgatg gctcagcgcc
    42901 agacttttgc tcagaaagcg caggcttttg agcaggatcg cgcacgtcgt tccaacgaag
    42961 aacgcggcaa actggttact cgtatccaga ctgctgtgaa atccgttgcc aacagccagg
    43021 atatcgatct ggttgttgat gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca
    43081 tcactgccga cgtactgaaa caggttaaat aagtaatgcc ttcaattcga ctggctgatt
    43141 tagcgcagca gttggatgca gaactacacg gtgatggcga tatcgtcatc accggcgttg
    43201 cgtccatgca atctgcacaa acaggtcaca ttacgttcat ggttaaccca aaataccgtg
    43261 agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg
    43321 cgaaaagtgc cgcgctggta gtgaagaatc cctaccagac ttacgcgcgc atggcgcaaa
    43381 ttttagatac cacgccgcag cccgcgcaga acattgcacc cagtgcggtg atcgacgcga
    43441 cggcgaagct gggtaacaac gtatcgattg gcgctaacgc ggtgattgag tccggcgttg
    43501 aactgggcga taacgtgatt atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg
    43561 gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca tgagatccag atcggtcaga
    43621 attgcctgat ccagtccgga acagtggtag gcgcagacgg ctttggttat gccaacgatc
    43681 gtggtaactg ggtgaagatc ccacagattg gtcgcgtaat tattggcgat cgcgtggaga
    43741 tcggtgcctg cacaaccatc gatcgcggcg cgctggatga cactgttatt ggcaatggcg
    43801 tgatcattga taaccagtgc cagattgcac ataacgtcgt gattggcgac aatacggcgg
    43861 ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg
    43921 gagccagcgt aatcaacggg catatggaaa tatgcgacaa agtgacggtt acgggcatgg
    43981 gtatggtgat gcgtcccatc actgaaccag gcgtctattc ctcaggcatt ccgctgcaac
    44041 ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa cattgatgac atgagcaagc
    44101 gtctgaaatc gcttgagcgc aaggttaatc aacaagacta acgttccatc ttttgttcgc
    44161 caaactttac ggcctgtctc attcttacga ttgcggcagg ccgtgttatt attgtcgttt
    44221 cttatatttt gacaggaaga gtatcttgac tactaacact catactctgc agattgaaga
    44281 gattttagaa cttctgccgc accgtttccc gttcttactg gtggatcgcg tgctggattt
    44341 tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca
    44401 gggccatttc cctggaaaac cgattttccc gggtgtgctg attctggaag caatggcaca
    44461 ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg gaaccgggtg agctgtacta
    44521 cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat
    44581 catggaagtc actttcgaaa aaacgcgccg cggcctgacc cgttttaaag gggttgctct
    44641 ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg
    44701 atacgtgatt gataaatccg cctttgtgca tccaaccgcc attgtggaag agggcgcgtc
    44761 aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg
    44821 tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt catactaaaa ttggccgcga
    44881 taatgagatt tatcagtttg cctccatcgg cgaagttaac caggatttga aatatgctgg
    44941 cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca
    45001 tcgtggcaca gtccagggcg gtggattgac gaaggtgggc agcgacaact tactgatgat
    45061 caacgcgcac attgcgcacg attgtacggt aggtaaccgc tgtattctcg ccaacaacgc
    45121 aacgctggcg ggtcacgtat cggttgacga cttcgcgatc atcggcggca tgaccgcagt
    45181 ccatcagttc tgcatcattg gtgcgcatgt gatggttggc ggctgctccg gtgtggcgca
    45241 ggacgtccct ccttatgtca ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat
    45301 cgaagggctg aagcgccgcg gattcagccg tgaggcgatt accgctatcc gcaatgcgta
    45361 taagctaatt tatcgtagcg gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact
    45421 ggcggaaaca tatccggaag tgaaagcctt taccgatttc tttgcacgct caacgcgcgg
    45481 tctgattcgt taatgactga ccagcgtcca ttaacgattg ccctggtcgc cggagaaacc
    45541 tccggcgata tcctgggggc cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc
    45601 cgctttgttg gtgttgccgg gccacgaatg caggctgaag gctgcgaagc ctggtacgaa
    45661 atggaagaac tggcggtgat gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg
    45721 catattcgtg ccgatctgac aaagcgtttt ggcgaactga agccagatgt ttttgttggt
    45781 attgatgcgc ccgacttcaa tattactctt gaaggtaacc tcaaaaagca gggtatcaaa
    45841 accattcatt acgtcagtcc atccgtctgg gcgtggcgac agaaacgcgt tttcaaaata
    45901 ggcagagcca ccgatctggt gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa
    45961 tacaatgtac cgtgccgctt tatcggtcat accatggctg atgccatgcc gttagatcca
    46021 gataaaaatg gtgcccgtga tgtgctgggg atcccttacg atgcccactg tctggcattg
    46081 ttgccgggca gccgtggtgc agaagtcgaa atgcttagtg ccgatttctt gaaaactgcc
    46141 cagcttttgc gccagacata tccggatctc gaaatcgtgg tgccgctggt gaatgccaaa
    46201 cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc cagacctgtc ggttcatttg
    46261 ctggatggaa tgggccgtga ggcgatggtc gccagcgatg cggcactact ggcgtcgggg
    46321 acggcagccc tggagtgtat gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag
    46381 ccttttacct tctggttggc gaagcggctg gtgaaaactg attatgtctc gctgccaaat
    46441 ctgctggcgg gcagagagtt agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa
    46501 ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa ccagccacgc gatgcacgat
    46561 accttccgtg aactgcatca gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc
    46621 gttctggagt tagcacaatg atcgaatttg tttatccgca cacgcagctg gttgcgggtg
    46681 tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg
    46741 acccggcgcg cccgattgcc gggctgaatg attccaaaaa gctgagcgaa aaacgccgtc
    46801 tggcgctcta tgaagagatc aaagagaaag cgttgagctg gagtctgggc cgcgcggaac
    46861 cccacgaaat cgacgagctg aacattcttc atgcgaccat gctggcgatg cagcgtgccg
    46921 tcgctgggct gcatattgcg ccggaatatg tgttgattga tggtaaccgc tgcccgaaat
    46981 taccgatgcc tgcgatggct gtggtgaaag gggatagccg cgtaccagaa atcagtgccg
    47041 cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc ggcgctggat attgttttcc
    47101 cgcaatatgg ttttgcccaa cataaagggt atccaaccgc ttttcatctg gaaaaactgg
    47161 ctgaatatgg cgcgactgaa catcatcgtc gcagctttgg gcctgtcaaa cgcgcactgg
    47221 gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac
    47281 cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc ttggccaaaa
    47341 ccgcaccgct ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg atcaccgatt
    47401 tcaccaacct ttgcggtctg gtgaagttct acggagcggg acatggcgca gggattaagc
    47461 ctatcgtcgg ggcagatttt aatgtccagt gcgacctgct gggtgatgag ttaacccacc
    47521 tgacggtact ggcggcgaac aataccggct atcagaatct ggcgttgctg atctcaaaag
    47581 cgtatcagcg cgggtacggt gccgccgggc cgatcatcga tcgcgactgg cttatcgaat
    47641 taaacgaagg gttgatcctt ctttccggcg ggcgcatggg cgacgtcgga cgcagtcttt
    47701 tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg
    47761 atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc tatctgcacg
    47821 cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgactaac gatgtgcgct
    47881 ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac gacggcttta
    47941 ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg cgtagcgaag
    48001 aggagatgtg tgagctgttt gccgacatcc cagaagccct tgccaacacc gttgagatcg
    48061 ccaaacgctg taacgtaact atgcgtcttg gtgaatactt cctgccgcag ttcccgaccg
    48121 gggacatgag caccgaagat tatctggtca agcgtgcaaa agaaggcctg gaagagcgtc
    48181 tggcattttt attccctgac gaggaagaac gtcttaaacg ccgcccggaa tatgacgaac
    48241 gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac ttcctcatcg
    48301 ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca ggccgtggct
    48361 ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat
    48421 ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct gacttcgacg
    48481 ttgacttctg tatggagaaa cgcgatcagg ttatcgaaca cgtggcggac atgtacggtc
    48541 gtgatgcggt atcgcagatt atcaccttcg gtacgatggc ggcgaaagcg gtgatccgcg
    48601 acgtaggccg cgtgctgggg catccgtacg gctttgtcga ccgtatctcg aaactgatcc
    48661 cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa
    48721 tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg
    48781 tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc aaaattaccg
    48841 attttgcgcc gctttattgc gatgaagagg gcaaacatcc ggtcacccag tttgataaaa
    48901 gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt acgctcacca
    48961 tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc gagccaccgc
    49021 tggatatcgc cgcgatcccg ctggatgaca agaaaagctt cgacatgctg caacgctcgg
    49081 aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc
    49141 aacctgactg cttcgaagat atgatcgcac tggtggcact gttccgccct ggtccgttgc
    49201 agtcagggat ggtggataac tttatcgacc gtaaacatgg tcgtgaagag atctcctatc
    49261 cggacgtgca gtggcagcat gaaagcctga aatcggtact ggagccaacc tacggcatta
    49321 tcttgtatca ggaacaggtc atgcagattg cccaggtgct ttctggttat accctcggtg
    49381 gcgcggatat gctgcgccgt gcgatgggta agaaaaagcc ggaagagatg gctaagcagc
    49441 gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa
    49501 tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa caaatcgcac tctgcggcct
    49561 atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatccggcg gagtttatgg
    49621 cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggcctg gtggatgagt
    49681 gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt taccatttcc
    49741 acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag
    49801 gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc gaactgtttg
    49861 atctctgcgc ccgtaccgac accaaaaagt taaaccggcg ggtgctggaa aaactgatca
    49921 tgtccggggc gtttgaccgt cttgggccac accgcgcggc gctgatgaac tcgctgggtg
    49981 atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag gccgatatgt
    50041 tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt
    50101 ggccggagca ggtggtatta gatggggaac gtgaaacgtt aggcctgtac ctgaccggac
    50161 accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta aggctgaaag
    50221 acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc
    50281 gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat gaccgttccg
    50341 ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa ttgctggaaa
    50401 aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc ggtgggctta
    50461 aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat gctcgcgggc
    50521 ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga ctccgtcagt
    50581 ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag agggcggatg
    50641 cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa
    50701 acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac taatacagga
    50761 atactatgag tctgaatttc cttgattttg aacagccgat tgcagagctg gaagcgaaaa
    50821 tcgattctct gactgcggtt agccgtcagg atgagaaact ggatattaac atcgatgaag
    50881 aagtgcatcg tctgcgtgaa aaaagcgtag aactgacgcg taaaatcttc gccgatctcg
    50941 gtgcatggca gattgcgcaa ctggcacgcc atccgcagcg tccttatacc ctggattacg
    51001 ttcgcctggc atttgatgaa tttgacgaac tggctggcga ccgtgcgtat gcagacgata
    51061 aagctatcgt cggtggtatc gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc
    51121 aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt tggtatgcca gcgccagaag
    51181 gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg ctttaagatg cctatcatca
    51241 cctttatcga caccccgggg gcttatcctg gcgtgggcgc agaagagcgt ggtcagtctg
    51301 aagccattgc acgcaacctg cgtgaaatgt ctcgccttgg cgtaccggta gtttgtacgg
    51361 ttatcggtga aggtggttct ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata
    51421 tgctgcaata cagcacctat tccgttatct cgccggaagg ttgtgcgtcc attctgtgga
    51481 agagcgccga caaagcgccg ctggcggctg aagcgatggg tatcatcgct ccgcgtctga
    51541 aagagctgaa actgatcgac tccatcatcc cggaaccgct gggcggcgct caccgtaacc
    51601 cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc ggatctggcc gatctcgatg
    51661 tgttaagcac tgaagattta aaaaatcgtc gttatcagcg cctgatgagc tacggttacg
    51721 cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg ccctttttta tcgccacggt
    51781 ttgagcaggc tatgattaag gaaggatttt ccaggaggaa cacatgaaca tcattgccat
    51841 tatgggaccg catggcgtct tttataaaga tgagcccatc aaagaactgg agtcggcgct
    51901 ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc gttgatttgc tgaaatttat
    51961 cgaacataac cctcgaattt gcggcgtgat ttttgactgg gatgagtaca gtctcgattt
    52021 atgcagcgat atcaatcaac ttaatgaata tctcccgctt tatgccttca tcaacaccca
    52081 ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg ctctggtttt ttgaatatgc
    52141 gctggggcag gcggaagata tcgccattcg tatgcgtcag tacaccaacg aatatcttga
    52201 taacattacg ccgccgttca cgaaagcctt gtttacctac gtcaaagagc ggaagtacac
    52261 cttttgtacg ccggggcata tgggcggcac cgcatatcaa aaaagcccgg ttggctgtct
    52321 gttttatgat tttttcggcg ggaataccct taaggctgat gtctctattt cggtcaccga
    52381 gcttggttcg ttgcttgacc acaccgggcc acatctggaa gcggaagagt acatcgcgcg
    52441 gacttttggc gcggaacaga gttatatcgt taccaacgga acatcgacgt cgaacaaaat
    52501 tgtgggtatg tacgccgcgc catccggcag tacgctgttg atcgaccgca actgccataa
    52561 atcactggcg catctgttga tgatgaacga tgtagtgcca gtctggctga aaccgacgcg
    52621 taatgcgttg gggattctgg gggggatccc gcgccgtgaa tttactcgcg acagcatcga
    52681 agagaaagtc gctgccacta cgcaagcaca atggccggtt catgcggtga tcaccaactc
    52741 cacctatgat ggtttgctct acaacaccga ctggatcaaa cagacgctgg atgtcccgtc
    52801 gatccacttc gattccgcct gggtgccgta tactcatttt catccaatct accagggtaa
    52861 aagtggtatg agcggcgagc gtgttgcggg aaaagttttc ttcgaaacgc aatcgaccca
    52921 caaaatgctg gcggcgttat cgcaggcttc actgatccac attaaaggcg agtatgacga
    52981 agaggcgttt aacgaagcct ttatgatgca taccaccacc tcgcccagtt atcccattgt
    53041 tgcttcggtt gagacggcgg cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa
    53101 ccgttcggtg gagcgggcgc tgcattttcg caaagaggtc cagcggctgc gggaagagtc
    53161 tgacggttgg tttttcgata tctggcaacc gccgcaggtg gatgaagccg aatgcaggcc
    53221 cgttgcgcct ggcgaacaat ggcacggctt taacgatgcg gatgccgatc atatgtttct
    53281 cgatccggtt aaagtcacta ttttgacacc ggggatggac gaacagggca atatgagtga
    53341 ggaggggatc ccggcggcgc tggtagcaaa attcctcgac gaacgtggga tcgtagtaga
    53401 gaaaaccggc ccttataacc tgctgtttct ctttagtatt ggcatcgata aaaccaaagc
    53461 aatgggatta ttgcgtgggt tgacggaatt taagcgctct tacgatctca acctgcggat
    53521 caaaaatatg ctgcccgatc tctatgctga agatcccgat ttctaccaca atatgcgtat
    53581 tcaggatctg gcgcaaggga tccataagct gattcgtaaa cacgatcttt ccggtttgat
    53641 gttgcgggca ttcgatactt tgccggaaat gatcatgacg ccacatcagg catggcagag
    53701 acagattaaa ggtgaagtag aaaccattgc gctggaacaa ctggtcggta gagtatcggc
    53761 aaatatgatc ctgccttatc caccgggcgt accgctgttg atgccaggag aaatgctgac
    53821 caaagagagc cgcacggtac tcgattttct actgatgctt tgtcccgtag ggcaacatta
    53881 ccccggtttt gaaacggata ttcacggcgc gaaacaggac gaagacggcg tttaccgcgt
    53941 acgagtccta aaaatggcgg gataacttgc cagagcggct tccgggcgag taacgtgctg
    54001 ttaacaaata aaggagacgt tatgctgggt ttaaaacagg ttcaccatat tgcgattatt
    54061 gcgacggatt atgcggtgag caaagctttc tactgcgata ttcttggttt cacgctgcaa
    54121 agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg atttggcgct taatgggcaa
    54181 tatgtgattg agcttttctc ttttccgttc ccgccggagc gccccagccg accggaagct
    54241 tgcggcctgc gtcatctggc ttttagcgtt gatgacatcg atgcggcagt ggcgcacctt
    54301 gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc catacacgca aaaacgcttc
    54361 accttcttta acgatccgga cgggctgccg ttggaactgt atgagcagta aggcttgtca
    54421 tcgccgcatt tgcccggtaa cgtgccgggc attgctactg taaaatcgca ccatcatgac
    54481 actcacgctc aatagacaac ttctcacctc acgccagatt ctggtggcct ttagcggcgg
    54541 gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg cggacggaaa atccgggcgt
    54601 cactctgcgc gctattcatg tgcatcacgg tttaagtgcc aatgccgatg cctgggttac
    54661 gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg gtggtcgaac gcgtacaact
    54721 tgcgcaagag gggctgggga ttgaggccca ggcgcggcag gcacgttatc aggcatttgc
    54781 ccgtacccta ttgcccggcg aagtgctggt caccgcgcaa catctcgacg atcaatgtga
    54841 aacctttctg ctggcgctaa aacgcggtag cggccctgcc gggctttcgg ctatggcgga
    54901 agtttcggag tttgctggaa cacggcttat ccgcccgttg cttgcccgca cgcgggggga
    54961 actggagcag tgggcattgg cgcatggttt acgctggatt gaagacgaaa gtaatcagga
    55021 cgactcatac gatcgtaact ttctgcgcct gcgcgtagtg ccgttattgc agcagcgttg
    55081 gccgcatttt gcggaagcaa cggcccgcag cgccacactt tgtgctgaac aagagagtct
    55141 gttggatgaa ctgctggcgg atgatttagc acactgccaa acgtcgcagg ggacgctgca
    55201 gattgcgcca atgctggcga tgagtgatgc ccgtcgcgcg gcgattattc gccgctggct
    55261 ggcagggcag aatgcaccga tgccttcccg cgacgcgctg gtgcgcatct ggcaggaagt
    55321 ggcgctggcg cgggaagatg cttccccctg tttacgtttg ggcgcctttg aaatccgccg
    55381 ctatcagtcg caactgtggt ggattaaatc cgtcaccggg caaagcgaaa ccattgtgct
    55441 gtggcagacg tggctacaac cgctgaaatt accggcgggg ctgggaaccg tacagcttac
    55501 tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc
    55561 gccaggattg ctgcatattg tcgggcgcaa cggcggacgt aagctgaaga aaatctggca
    55621 agagttaggc gtgccgccgt ggctacgtga caccacgcca ctgctgtttt atggagaaac
    55681 gctgattgcg gcggcagggg tatttgtgac gcaagaaggt gtggctgaag gtgagaatgg
    55741 cgtaagtttt gtctggcaga aaacgcttag ttaagtgaaa gccggataag acgcatcaaa
    55801 cgtcgcatcc ggcgaaagtc aatcaggatt cgcttaccac caccgtaccg atttcagggt
    55861 ggctaaagct ggtaatttta tccagacgca gctcgcgggt ttcgccagcg gcctcgacga
    55921 ccagatactc cacatttttg cgggagacta aatcactggc ttttgcctgc aatttttcgc
    55981 catctttcag ctcaagtgtc agcattaaat gatgctggca ggcgagctca agattatcgt
    56041 aatcatcaca attaattggt tgatacgtat cattcattga cataatcgct caccagtaag
    56101 tttgccgcag cgtatgctgc tttttccctg acagcctcag aaagggcgtc gtcggcagcc
    56161 atttcattca gcactttcaa aacgcagccc agcgcgtccg gaacgtatcc taagtctccg
    56221 ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat atttttccac atgccctcct
    56281 gtcagcactc tgacttaacc gtggatgcaa gtctaagcct acgaagataa actctgtttc
    56341 gcaaggtgac tataccacac tcatttctgc aatatcagcg ccgcaactgc acgtattccg
    56401 ttacaatggc ctcctgattc gaaaggagtt ttcttatggc gcttaaagcg acaatttata
    56461 aagcgacggt taatgtggcc gatctcgacc gcaaccagtt tctcgatgcc tctctgacgc
    56521 tggcgcgcca cccttcagaa acccaggagc gtatgatgct gcgcttgctg gcgtggctga
    56581 aatatgctga tgaacgtctg caatttaccc gtggtttgtg tgccgatgat gagccggaag
    56641 cgtggctgcg taacgatcac ctgggcattg atttgtggat tgagctgggg ctgccggatg
    56701 agcgacgaat taagaaagcc tgcacccagg ccgcagaagt ggcgctgttt acctataata
    56761 gtcgggcggc gcaaatctgg tggcagcaaa atcagagcaa atgtgtgcag tttgccaatc
    56821 tttccgtctg gtatctggac gatgaacaac tggcgaaagt aagcgccttt gccgatcgta
    56881 ccatgacgct gcaggcaacg attcaggatg gtgttatctg gttatcggat gataagaata
    56941 atctggaagt taacttaacc gcctggcaac aaccttcatg attgtgattt cccgacatgt
    57001 tgctattccc gatggtgagc ttgagatcac cgccattcgt gcgcagggcg ctggcgggca
    57061 gcatgttaat aagacctcaa cggctattca tctgcgtttt gacattcggg cgtccagcct
    57121 gccagagtat tacaaagagc gtctgctcgc cgccagccat catttgatca gcagtgatgg
    57181 cgtgattgtc attaaggcac aggaataccg cagtcaggaa ctgaaccgcg aagccgctct
    57241 ggcccggctg gtggcagtga ttaaagattt aacaaaagaa caaaaagccc gacgacccac
    57301 gcggcccacc cgtgcatcga aagagcgcag gctggcatcg aaagcacaaa aatcaagcgt
    57361 gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa aaagaaggaa tggatggtga
    57421 aaaaagcgat agtgacagcg atggctgtaa tcagcctctt tactctgatg ggatgtaata
    57481 atcgggccga agtcgatacg ctttctccgg cgcaggctgc cgaactgaaa ccgatgccgc
    57541 aaagttggcg cggcgtgcta ccgtgtgccg attgcgaagg aatcgaaatc tctctgttcc
    57601 tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct cggtgctcgt gaagaacctt
    57661 cctccttcgc ttcctacggt acatgggcgc gaaccgctga caagctggta ttaaccgaca
    57721 gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg
    57781 aaggcaatcc aattgaatcg cagttcaact atacgctgga accggcacaa tccagtttac
    57841 ctatgacgcc gatgaccctg cgaggcatgt atttttatat ggctgatgcg gcgaccttca
    57901 ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa cgcagagctg gagcgtagct
    57961 acctggctgc gcgcggtcac agtgaaaaac cggtgttact gtcagtagaa ggtcacttta
    58021 cgcttgaggc taatccggat actggtgcgc caaccaaagt attagcgccc gatacggcgg
    58081 gtaaatttta cccgaataag gattgcagta gtttggggca gtaaacctgc ttaaggcaga
    58141 aacaaacgca aaattgcctg atgcgctgcg cttatcaggc ctacagcata taatgcaaca
    58201 ttttgaatta gcacgatttt gtaggccgga taaggcgttt acgccgcatc cggcattttg
    58261 tgcacgaggc tggctttatc actgagtcag cccaacaaag cgacccgcct tgatataaat
    58321 cccaggcttc aggtgcccaa catactgtaa ctgcgtttct ggcttaaacg cagagacatc
    58381 accaatacgc atatgcatca gatcggcagg gctaatccag cccgattgcg ccagtgtaag
    58441 tggatgcccg gatttggcaa atgcatcagt gacaaattcc gaacaaaacc acgacttttt
    58501 gtctccttcg cacacactgc ttaactgcgc tttcgccagg tcgctgacgc actgttggcg
    58561 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
    58621 ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa acgcggtgat
    58681 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
    58741 cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt
    58801 attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
    58861 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtgc
    58921 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
    58981 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
    59041 acaagccgaa agtaaaagaa agctggggag aagcaggcgg cagtacgcct ttggtttatc
    59101 cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc tggcatcagc
    59161 ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac tgtttctcgc
    59221 cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg cccagcacaa
    59281 tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga cgctctttgc
    59341 ggtcatccag cagcacttcg ataccttgtg cacgcagttc gctgtacagt ttctcagcaa
    59401 gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc tggaacggcg
    59461 cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc gccgcagcta
    59521 ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta cggccatctt
    59581 caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg aagatgtgac
    59641 caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt ggatcgccag
    59701 ccaccacgtt acggatatct gcaacttccg gggtagcgac atcgcgatcc cagttgatac
    59761 cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc gccgcaacgg
    59821 tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc ggaccggctt
    59881 taaccacagc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca acctgcggca
    59941 gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg caccagcagc gcaaccagcg
    60001 ggaagctact gccttcaacc gctttaacca gcagagtctt aaccgttttc tcaatcggca
    60061 gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca accagcgtca
    60121 tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc agttcaatgt
    60181 tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc tgcgccagca
    60241 cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcctgt acggcgcgga
    60301 aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca tcgtaggttt
    60361 cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg aattcgcggg
    60421 aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa cttggtctgg atctgataga
    60481 agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca gtgataactt
    60541 cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa cgcagcagtt
    60601 ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc tgaaccaccg
    60661 gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc acgcacgatg ttttcgactt
    60721 ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc agcttgcgga
    60781 tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca ggtgtctcct
    60841 tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag ttggaaggta
    60901 gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt agtttaccag tgcaaaagaa
    60961 atgtcaaaag agaagggcgt gaatttaacg cggttccagc gcaaagactt caaaacctgc
    61021 gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag acggcatagg ttttgcccgt
    61081 ttcctcacct ttacgatagg ccgggcgcgg gtcctgcgcc agtacttcgc ggataaacag
    61141 cgttaactgc ggataacgct tctccagcgt caaaagctgc ttttcgacct ctgcggtaaa
    61201 actcaccgcc atctctgcag ctggcgcact ttgcgcatag ctggcactgg catcgggaag
    61261 cgattcggca aaggggagat acggtttgat atccactacc ggcgtaccat cgaccagatc
    61321 cagactgccg agcttcagaa tcacgctgcc tttatggcaa acaacctctt tcagctctac
    61381 cagcgacatg ccaattgggt tagggcggaa agtagagcgt gttgcgaaaa cccccattct
    61441 ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc cagccgcctt ccatcgtttg
    61501 atgaaagacg aaaaggatcc ataaatggct gaatgcttcc aggccgcgaa cggcgtcggc
    61561 ctggttgtag gaagcaatga gatgcagttc gccgttggcg ctttttacca gacctggctg
    61621 gcgcggaacg gcgaattttt ctttataggg cgagcgaata acgcctattt gctcgaactg
    61681 gaaactgctc atttcgccgt aatgttaagc gcagagccga tacatacagc ctgacgatag
    61741 cagcccggcg taccgctggt gacttcgcag ctatgcagta atacagcatt ggctttcatt
    61801 ttagaggcat tgatttgcat ctgcttacgt gcggttggaa tgctcggcgg agagtcctga
    61861 ttagaggcct ggcaagagtc gccactgact tcaccgagat cgcggaacgg tttgccgact
    61921 aattcttctg cattggtata aattcggacc ggcgtggcgc gcggcgcttt cggttttgca
    61981 ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt cgacagggga tctgcttaac
    62041 atggaacagc cgcttagcat gagtgctact aaacagatcg gtaaagcacg catagtattt
    62101 cctcaatgta tgatcaaaac gtcaatattg aatcaggagc ttgtaaaaat gacaagacgg
    62161 gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac agcctaaaaa ttaccagcct
    62221 ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt aaacttcatc agactgataa
    62281 gcctggacga atttcttcac gttctcggcg tctttgttat cttcacgcgt tacgatcagg
    62341 tttacgtatg gggagtcttt atcttcgaca aagataccgt ctttcgccgg agtcaggcca
    62401 atctggctgg cataggtggt attgataact gccagagcga tttgcgcgtc gtccagagaa
    62461 cgcggcagtt gcggtgcttc cagttcaaca attttcagat tttttgggtt ctcaacaaca
    62521 tcaagaacgg tcggcagcag gccaacgcca tctttcagtt tgatcaagcc cactttttgc
    62581 agcagcagca gtgaacgacc aagattagtt ggatcgtttg gcacggcaac ctgcgaacca
    62641 tcctgcagtt catccagtga tttgattttc ttggagtaac cagcaatcgg ataaacaaaa
    62701 gtgttgccta ctgcgaccag tttgtagcca cgatctttca gttgctgatc aaggtacggt
    62761 ttatgctgga aggcgttggc gtcgatatcg cctttgctca atgcttcgtt tggcagaaca
    62821 tagtcgttga aggttaccag ctcaacgtcc aggccatatt tgtctttcgc aactttctgc
    62881 gcgacttctg ctacctgctg ttcggcacca acaatcacgc cgactttaat gtggtttgga
    62941 tctttttcat cctgaccgca gcctaccagt gccagtgatc caatcagggc tcccactgcc
    63001 gcaaaggttt tgaatttgaa cgccatacct tattccttct tcaattattt atgttgtgtt
    63061 gaacgttact tgcgagtgac agcccggacg atgcggtcgc ctgcgaactg aattaaataa
    63121 accagaatga ccagcaatac cagtaccgta ttcatcaccg tcgcgttgta gccgatgtag
    63181 ccatactgat agccaatctg acctaaacca ccggcaccga ctgcaccacc catcgcggaa
    63241 taaccgacca gggtaatcag ggtgatagtt gccgcattca ccagacccgg caacgcttcc
    63301 ggtaacagca ctttacagac gatctgcatt ggcgtggcac ccattgcgcg ggaagcttca
    63361 attaacccgg ttgggatctc cagcagagcg ttctcgacca tacgggcaat aaacggtgct
    63421 gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa tcgatgtacc gacaataacg
    63481 cgggtaaacg gaatcatcca taccagcaag ataatgaacg ggatggaacg gaaaatgttc
    63541 acaatcgcag aaacggtacg atacagcttc gcgttagcaa taatttgccc cggacgcgtg
    63601 acataaagca gaacgccaac cggcagacca atcacaaacc caaaaaatcc ggatacgaag
    63661 gtcattgcca gcgtttccca tacgccacga accagcagcc acatcatcgg ctcagacata
    63721 acccagtacc tctactttta catggtgttc ctgcagccag gcaatggcgg cttgcgtatc
    63781 ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat ttaacgccac cggcgtagtc
    63841 catctgcgcg ctaataatgt tgttgttgac gttgaaacga cgcgcggttt cagaaagcag
    63901 tggggcatcg accgattgac cggtaaactc cagacgcagc atcggcacgc agtcagtaaa
    63961 tggctccgtt tgcagacgtt cctggtaatc ttccgggata tccagatgca gggtcgactg
    64021 aataaacttc tgcgccagcg gcgttttcgg atgcgagaac acttcactta ccgtgtcctg
    64081 ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca caaatacgct tcacaacgtc
    64141 catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg cggttaatgt ctttcagcag
    64201 ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg gtggcttcat cacacagcaa
    64261 tactttggga ttgctggcta atgcacgggc aatcgccaca cgttgtttct gcccaccgga
    64321 aagattcgac gggtagctat catgcttatc gccaagacca accaatgaca gcaattccgt
    64381 cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc tccagcggca gagccacgtt
    64441 gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg aaaatcatac caatctggcg
    64501 gcgagctttg gtcaactcgg attctgacag cgtggtcaat tcctggccat cgaccagcac
    64561 gctaccctcg gttgggcgct ccagcaggtt tacacaacgt ataagcgtac tcttacccgc
    64621 gcctgaggca ccgataacgc cataaatttg tccagctggc acatgcaggc tgacgttgtt
    64681 caacgcctgg atggtgcggg tgccctggtg gaacactttg gtgatattcg aaagttttat
    64741 cattgattat ttattatcgt cattaagtta gtcgtagcat ctcgaatgcc tgaaacgggc
    64801 aacgccgtca atgaaatgga tgttaaggca tccagacgtc taaatcaatc aggtttatgc
    64861 gaagagcact ttcttgcagg tcgaaacatg cgatactagc gtcccatgcc ttattaagga
    64921 gctataaaag gtggcgaaga gcgtacccgc aatttttctt gaccgtgatg gcaccattaa
    64981 tgtcgatcac ggctatgtcc atgagatcga caactttgaa tttatcgacg gtgttattga
    65041 cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg gtagtaacca accagtctgg
    65101 cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg ctgaccgagt ggatggactg
    65161 gtcgctggcg gaccgagatg tcgatctgga tggtatctat tattgcccgc atcatccgca
    65221 ggggagtgtt gaagagtttc gccaggtctg cgattgccgc aaaccacatc cggggatgct
    65281 tttgtcagca cgcgattatt tgcatattga tatggccgct tcttatatgg tgggcgataa
    65341 attagaagat atgcaggcag cggttgcggc gaacgtggga acaaaagtgc tggtgcgtac
    65401 gggtaaacct attacacctg aagcagaaaa cgcggcagat tgggtgttaa atagcctggc
    65461 agacctgccg caagcgataa aaaagcagca aaaaccggcg caatgattaa aagatgaacg
    65521 gttgaaataa aaatgcattt ttccgcttgt cttcctgagc cgactcccta taatgcgcct
    65581 ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa
    65641 aatcctgaaa ttcagggttg actctgaaag aggaaagtgt gatatacgcc acctcgcgac
    65701 agtgagctga aagctgcgtc gcaactgctc tttaacaatt tatcagacaa tctgtgtggg
    65761 cactcgaaga tacggattct taacgtcgca agacgataaa tgaataccaa gtctcaagag
    65821 tgaacacgta attcattacg aagtttaatt ctttgagcat caaactttta aattgaagag
    65881 tttgatcatg gctcagattg aacgctggcg gcaggcctaa cacatgcaag tcgaacggta
    65941 acaggaagcg cttgctgctt cgctgacgag tggcggacgg gtgagtaatg tctgggaaac
    66001 tgcctgatgg agggggataa ctactggaaa cggtagctaa taccgcataa cgtcgcaaga
    66061 ccaaagaggg ggaccttcgg gcctcttgcc atcggatgtg cccagatggg attagcttgt
    66121 tggtggggta acggctcacc
//
LOCUS       DAECNO010000008        65614 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.008, whole genome shotgun sequence.
ACCESSION   DAECNO010000008 DAECNO010000000
VERSION     DAECNO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.008"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KKO08_000534"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="KKO08_000534"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBD6316920.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KKO08_000535"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="KKO08_000535"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBD6316921.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KKO08_000536"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="KKO08_000536"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBD6316922.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KKO08_000537"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="KKO08_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBD6316923.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KKO08_000538"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="KKO08_000538"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBD6316924.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KKO08_000539"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="KKO08_000539"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBD6316925.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="KKO08_000540"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="KKO08_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBD6316926.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KKO08_000541"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="KKO08_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD6316927.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KKO08_000542"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="KKO08_000542"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBD6316928.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KKO08_000543"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="KKO08_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBD6316929.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KKO08_000544"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="KKO08_000544"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBD6316930.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="KKO08_000545"
     CDS             complement(15665..16702)
                     /locus_tag="KKO08_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD6316931.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="KKO08_000546"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="KKO08_000546"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD6316932.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KKO08_000547"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="KKO08_000547"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBD6316933.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="KKO08_000548"
     CDS             19444..19884
                     /locus_tag="KKO08_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBD6316934.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KKO08_000549"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="KKO08_000549"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD6316935.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21601)
                     /locus_tag="KKO08_000550"
                     /pseudo
     CDS             complement(20611..21601)
                     /locus_tag="KKO08_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KKO08_000551"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="KKO08_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBD6316936.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KKO08_000552"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="KKO08_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBD6316937.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KKO08_000553"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="KKO08_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBD6316938.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KKO08_000554"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="KKO08_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBD6316939.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KKO08_000555"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="KKO08_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBD6316940.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KKO08_000556"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="KKO08_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBD6316941.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KKO08_000557"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="KKO08_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBD6316942.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KKO08_000558"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="KKO08_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBD6316943.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="KKO08_000559"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="KKO08_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBD6316944.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KKO08_000560"
     CDS             complement(30985..32088)
                     /gene="livJ"
                     /locus_tag="KKO08_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBD6316945.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KKO08_000561"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="KKO08_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBD6316946.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KKO08_000562"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="KKO08_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBD6316947.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KKO08_000563"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="KKO08_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBD6316948.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KKO08_000564"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="KKO08_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBD6316949.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="KKO08_000565"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="KKO08_000565"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBD6316950.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="KKO08_000566"
     CDS             37412..37681
                     /locus_tag="KKO08_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBD6316951.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="KKO08_000567"
     CDS             complement(37684..38043)
                     /locus_tag="KKO08_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBD6316952.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="KKO08_000568"
     CDS             38184..38810
                     /locus_tag="KKO08_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBD6316953.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="KKO08_000569"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="KKO08_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBD6316954.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KKO08_000570"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="KKO08_000570"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBD6316955.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="KKO08_000571"
     CDS             41650..42315
                     /locus_tag="KKO08_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBD6316956.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="KKO08_000572"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="KKO08_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBD6316957.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="KKO08_000573"
     CDS             complement(42949..44166)
                     /locus_tag="KKO08_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6316958.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="KKO08_000574"
     CDS             44300..45349
                     /locus_tag="KKO08_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD6316959.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="KKO08_000575"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="KKO08_000575"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBD6316960.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="KKO08_000576"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="KKO08_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBD6316961.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="KKO08_000577"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="KKO08_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBD6316962.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="KKO08_000578"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="KKO08_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBD6316963.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="KKO08_000579"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="KKO08_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBD6316964.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="KKO08_000580"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="KKO08_000580"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBD6316965.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="KKO08_000581"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="KKO08_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBD6316966.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="KKO08_000582"
     CDS             complement(51544..51903)
                     /locus_tag="KKO08_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBD6316967.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="KKO08_000583"
     CDS             complement(51900..52175)
                     /locus_tag="KKO08_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBD6316968.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="KKO08_000584"
     CDS             complement(52234..53358)
                     /locus_tag="KKO08_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6316969.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KKO08_000585"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="KKO08_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBD6316970.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="KKO08_000586"
     CDS             complement(56090..57157)
                     /locus_tag="KKO08_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6316971.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="KKO08_000587"
     CDS             complement(57523..59145)
                     /locus_tag="KKO08_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD6316972.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="KKO08_000588"
     CDS             complement(59407..60996)
                     /locus_tag="KKO08_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBD6316973.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61381..62412
                     /locus_tag="KKO08_000589"
                     /pseudo
     CDS             61381..62412
                     /locus_tag="KKO08_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62499..62975)
                     /locus_tag="KKO08_000590"
     CDS             complement(62499..62975)
                     /locus_tag="KKO08_000590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316974.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="KKO08_000591"
     CDS             complement(62968..63732)
                     /locus_tag="KKO08_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBD6316975.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="KKO08_000592"
     CDS             63978..64739
                     /locus_tag="KKO08_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD6316976.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65346)
                     /locus_tag="KKO08_000593"
     CDS             complement(64846..65346)
                     /locus_tag="KKO08_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6316977.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
BASE COUNT        15937 a        17620 c        16161 g        15896 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gctagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcct
//
LOCUS       DAECNO010000009        60419 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.009, whole genome shotgun sequence.
ACCESSION   DAECNO010000009 DAECNO010000000
VERSION     DAECNO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.009"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..322
                     /locus_tag="KKO08_000594"
     CDS             <1..322
                     /locus_tag="KKO08_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6316978.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="KKO08_000595"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="KKO08_000595"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBD6316979.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="KKO08_000596"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="KKO08_000596"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBD6316980.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KKO08_000597"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="KKO08_000597"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBD6316981.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="KKO08_000598"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="KKO08_000598"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBD6316982.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="KKO08_000599"
     CDS             complement(3726..4955)
                     /locus_tag="KKO08_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBD6316983.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="KKO08_000600"
     CDS             complement(5019..5459)
                     /locus_tag="KKO08_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD6316984.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="KKO08_000601"
     CDS             complement(5564..6334)
                     /locus_tag="KKO08_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6316985.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="KKO08_000602"
     CDS             complement(6331..7257)
                     /locus_tag="KKO08_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6316986.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="KKO08_000603"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="KKO08_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBD6316987.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KKO08_000604"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="KKO08_000604"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBD6316988.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="KKO08_000605"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="KKO08_000605"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBD6316989.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KKO08_000606"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="KKO08_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBD6316990.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="KKO08_000607"
     CDS             12964..13311
                     /locus_tag="KKO08_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBD6316991.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="KKO08_000608"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="KKO08_000608"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBD6316992.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="KKO08_000609"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="KKO08_000609"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBD6316993.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="KKO08_000610"
     CDS             complement(15131..15493)
                     /locus_tag="KKO08_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBD6316994.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KKO08_000611"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="KKO08_000611"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBD6316995.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="KKO08_000612"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="KKO08_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KKO08_000613"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="KKO08_000613"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBD6316996.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="KKO08_000614"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="KKO08_000614"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBD6316997.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="KKO08_000615"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="KKO08_000615"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBD6316998.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="KKO08_000616"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="KKO08_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBD6316999.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="KKO08_000617"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="KKO08_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBD6317000.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="KKO08_000618"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="KKO08_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBD6317001.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="KKO08_000619"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="KKO08_000619"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBD6317002.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="KKO08_000620"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="KKO08_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBD6317003.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="KKO08_000621"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="KKO08_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBD6317004.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="KKO08_000622"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="KKO08_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="KKO08_000623"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="KKO08_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBD6317005.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="KKO08_000624"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="KKO08_000624"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBD6317006.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="KKO08_000625"
     CDS             complement(36348..36386)
                     /locus_tag="KKO08_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317007.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="KKO08_000626"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="KKO08_000626"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBD6317008.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="KKO08_000627"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="KKO08_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBD6317009.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="KKO08_000628"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="KKO08_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBD6317010.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="KKO08_000629"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="KKO08_000629"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBD6317011.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="KKO08_000630"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="KKO08_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBD6317012.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="KKO08_000631"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="KKO08_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBD6317013.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="KKO08_000632"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="KKO08_000632"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBD6317014.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="KKO08_000633"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="KKO08_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBD6317015.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="KKO08_000634"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="KKO08_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBD6317016.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="KKO08_000635"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="KKO08_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBD6317017.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="KKO08_000636"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="KKO08_000636"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD6317018.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="KKO08_000637"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="KKO08_000637"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBD6317019.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="KKO08_000638"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="KKO08_000638"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBD6317020.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="KKO08_000639"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="KKO08_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBD6317021.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="KKO08_000640"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="KKO08_000640"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBD6317022.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAGQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="KKO08_000641"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="KKO08_000641"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBD6317023.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="KKO08_000642"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="KKO08_000642"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBD6317024.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="KKO08_000643"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="KKO08_000643"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBD6317025.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="KKO08_000644"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="KKO08_000644"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBD6317026.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="KKO08_000645"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="KKO08_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBD6317027.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="KKO08_000646"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="KKO08_000646"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBD6317028.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="KKO08_000647"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="KKO08_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBD6317029.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14475 a        16734 c        14848 g        14362 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg cccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccaag gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgaacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagcg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactggac gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagaattg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggcaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcaa
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt cctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac agctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgactctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAECNO010000010        58484 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.010, whole genome shotgun sequence.
ACCESSION   DAECNO010000010 DAECNO010000000
VERSION     DAECNO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.010"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="KKO08_000648"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="KKO08_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBD6317030.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="KKO08_000649"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="KKO08_000649"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBD6317031.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="KKO08_000650"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="KKO08_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBD6317032.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KKO08_000651"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="KKO08_000651"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBD6317033.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KKO08_000652"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="KKO08_000652"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBD6317034.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="KKO08_000653"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="KKO08_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBD6317035.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAELFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="KKO08_000654"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="KKO08_000654"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBD6317036.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="KKO08_000655"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="KKO08_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="KKO08_000656"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="KKO08_000656"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBD6317037.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="KKO08_000657"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="KKO08_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBD6317038.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KKO08_000658"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="KKO08_000658"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBD6317039.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="KKO08_000659"
     CDS             complement(13538..13933)
                     /locus_tag="KKO08_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBD6317040.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KKO08_000660"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="KKO08_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBD6317041.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="KKO08_000661"
     CDS             complement(15220..15447)
                     /locus_tag="KKO08_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBD6317042.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="KKO08_000662"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="KKO08_000662"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBD6317043.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="KKO08_000663"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="KKO08_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBD6317044.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KKO08_000664"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="KKO08_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBD6317045.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="KKO08_000665"
     CDS             complement(18383..19480)
                     /gene="mltA"
                     /locus_tag="KKO08_000665"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBD6317046.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19689..19765
                     /locus_tag="KKO08_000666"
     tRNA            19689..19765
                     /locus_tag="KKO08_000666"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            19799..19875
                     /locus_tag="KKO08_000667"
     tRNA            19799..19875
                     /locus_tag="KKO08_000667"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19833..19835,aa:Met,seq:cat)
     gene            complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="KKO08_000668"
     CDS             complement(19950..21203)
                     /gene="amiC"
                     /locus_tag="KKO08_000668"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBD6317047.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21435..22766
                     /gene="argA"
                     /locus_tag="KKO08_000669"
     CDS             21435..22766
                     /gene="argA"
                     /locus_tag="KKO08_000669"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBD6317048.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22828..24654)
                     /gene="recD"
                     /locus_tag="KKO08_000670"
     CDS             complement(22828..24654)
                     /gene="recD"
                     /locus_tag="KKO08_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBD6317049.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24654..28196)
                     /gene="recB"
                     /locus_tag="KKO08_000671"
     CDS             complement(24654..28196)
                     /gene="recB"
                     /locus_tag="KKO08_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBD6317050.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="KKO08_000672"
     CDS             complement(28189..31077)
                     /gene="ptrA"
                     /locus_tag="KKO08_000672"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBD6317051.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31253..34621)
                     /gene="recC"
                     /locus_tag="KKO08_000673"
     CDS             complement(31253..34621)
                     /gene="recC"
                     /locus_tag="KKO08_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBD6317052.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34634..34966)
                     /locus_tag="KKO08_000674"
     CDS             complement(34634..34966)
                     /locus_tag="KKO08_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBD6317053.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(34951..35358)
                     /locus_tag="KKO08_000675"
     CDS             complement(34951..35358)
                     /locus_tag="KKO08_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBD6317054.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35355..35918)
                     /locus_tag="KKO08_000676"
     CDS             complement(35355..35918)
                     /locus_tag="KKO08_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBD6317055.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35909..36379)
                     /locus_tag="KKO08_000677"
                     /pseudo
     CDS             complement(35909..36379)
                     /locus_tag="KKO08_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="KKO08_000678"
     CDS             complement(36563..37357)
                     /gene="thyA"
                     /locus_tag="KKO08_000678"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBD6317056.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="KKO08_000679"
     CDS             complement(37364..38239)
                     /gene="lgt"
                     /locus_tag="KKO08_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBD6317057.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPDSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="KKO08_000680"
     CDS             complement(38390..40636)
                     /gene="ptsP"
                     /locus_tag="KKO08_000680"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD6317058.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="KKO08_000681"
     CDS             complement(40649..41179)
                     /gene="rppH"
                     /locus_tag="KKO08_000681"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBD6317059.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINLQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41864..42553
                     /gene="mutH"
                     /locus_tag="KKO08_000682"
     CDS             41864..42553
                     /gene="mutH"
                     /locus_tag="KKO08_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBD6317060.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42622..43335
                     /locus_tag="KKO08_000683"
     CDS             42622..43335
                     /locus_tag="KKO08_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD6317061.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43473..43691
                     /gene="ygdR"
                     /locus_tag="KKO08_000684"
     CDS             43473..43691
                     /gene="ygdR"
                     /locus_tag="KKO08_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBD6317062.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43799..44839
                     /locus_tag="KKO08_000685"
     CDS             43799..44839
                     /locus_tag="KKO08_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBD6317063.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="KKO08_000686"
     CDS             complement(44872..46062)
                     /gene="lplT"
                     /locus_tag="KKO08_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBD6317064.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46055..48214)
                     /gene="aas"
                     /locus_tag="KKO08_000687"
     CDS             complement(46055..48214)
                     /gene="aas"
                     /locus_tag="KKO08_000687"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBD6317065.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48800..49831
                     /gene="galR"
                     /locus_tag="KKO08_000688"
     CDS             48800..49831
                     /gene="galR"
                     /locus_tag="KKO08_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBD6317066.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="KKO08_000689"
     CDS             complement(49838..51100)
                     /gene="lysA"
                     /locus_tag="KKO08_000689"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBD6317067.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51222..52157
                     /gene="lysR"
                     /locus_tag="KKO08_000690"
     CDS             51222..52157
                     /gene="lysR"
                     /locus_tag="KKO08_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBD6317068.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="KKO08_000691"
     CDS             complement(52144..52836)
                     /gene="ygeA"
                     /locus_tag="KKO08_000691"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBD6317069.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52965..54383)
                     /gene="araE"
                     /locus_tag="KKO08_000692"
     CDS             complement(52965..54383)
                     /gene="araE"
                     /locus_tag="KKO08_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBD6317070.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="KKO08_000693"
     CDS             complement(54698..55459)
                     /gene="kduD"
                     /locus_tag="KKO08_000693"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBD6317071.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="KKO08_000694"
     CDS             complement(55489..56325)
                     /gene="kduI"
                     /locus_tag="KKO08_000694"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBD6317072.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56612..57793)
                     /locus_tag="KKO08_000695"
     CDS             complement(56612..57793)
                     /locus_tag="KKO08_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBD6317073.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58048..58484
                     /locus_tag="KKO08_000696"
                     /pseudo
     CDS             58048..58484
                     /locus_tag="KKO08_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT        14389 a        15767 c        14325 g        14003 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 ctggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gataaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagcctgg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gaattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttccaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggca gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatgacgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccaaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgtcggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgctgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgagt
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat agcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcagggggc ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcaccctgtc atatccgtaa
    18361 aaacggcata cagaatatca catcagccgc taaagacgtt acctgcgccc ggagccgttt
    18421 tcagcaccca aacccgtcca tagtggttgt accaacctgc gcggtgtccg gcttccggcc
    18481 cgatcccttg atagatatcg aagtgttggc ctttgattgc gcccccgaca tccaacgcca
    18541 ccatcagacg caattcgtac tgaccgttaa atttgccgtt attatccagc agcggcactt
    18601 ctgccagcaa ggtagtacct ggcggaataa ttgaacgatc ggaggcaaca gaagcacggc
    18661 caaccagcgg caccgcactt gcccctttca ccggtgcaaa agattgcggt ttaaagaaga
    18721 cgaaagacgg gttctgttcc agcagctcgc gaacctcggc ttcactgtgt gtttcgcccc
    18781 aatgacgaat cgcctgcatc gacatatctt cttttttcac ttcgccacgg tcgatcagca
    18841 ccttaccaat gctgcgatag gcatgaccgt ttttccctgc atagctgaaa aagttaagcg
    18901 gactgccatc accaaagtcg atatacccac taccctgcac atccataatg aagttatcca
    18961 tcagagagtt gctgtaagcg aggatgtatt tatcgcccaa tgccccggcg tagatctccg
    19021 cacgagacgg cagacgacca cgttttggcg gcatacggta aataggatac tggaactcgc
    19081 cctggcgggt atggcgcgcc tgaattaccg gcgtgtaata gcctgtaaac tgcacgttac
    19141 catagttgtc ggcaccttcc atctgccaag catcaatgcc gaactggcgc atattgcggg
    19201 tatcaccgcc tgcgcgcagc cactcttgca ccgcgttata aacattactc tggttgccat
    19261 acagacgcgg tgacgaatta cggatatggt taatttgctc ggcaaaatca ccggcgttaa
    19321 tcggcgcgcc aacggcatct ggctggttca ccagagagaa aggctgggta aatttcccgt
    19381 ctttatattg ctgtccgcga tcggttggtt tggaagagca ggcggcaagc attgccacaa
    19441 ccgtgcccat aagaaggtac tttacccaac gtcctttcat tgttctcttc ttcaggttaa
    19501 aaaataaggc gcaacgaaga taacaaaccg ccgcgtttaa tgaaatgagc agcctctccc
    19561 tgacgcaaat tttgcacaaa aaataggctt tagtggtttg tttttgttca aaatcatgct
    19621 aaatccgtga tcggggtaaa aaaaaggttg catgaaaacg cgagcggagt atagtgcgca
    19681 tccacggacg cggggtggag cagcctggta gctcgtcggg ctcataaccc gaaggtcgtc
    19741 ggttcaaatc cggcccccgc aaccaatcaa atttgatgaa gtaaaagcag tacggtgacg
    19801 cggggtggag cagcttggta gctcgtcggg ctcataaccc gaaggtcgtc ggttcaaatc
    19861 cggcccccgc aaccaattat tgaacaccct aacgggtgtt tttttgtttc tgatctccca
    19921 taaaaaaagc gccattcagc gcctttttat catccccttc tcgccagcgt tgccccatcg
    19981 gcaaaatacg ctttaatccc cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc
    20041 gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac cagaatggag
    20101 ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt tttatgcagc
    20161 ttgttgattt ttccgagctt attcagtacc gctttaccaa acttaaggct gtcagcaata
    20221 gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc gcttttgctc
    20281 acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc cgcagtactg
    20341 gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctgacg actggtaaag
    20401 gcgtcggcat ggatagagac aaacaggtca gcacgctgtt tctgggcttt tgctacgcgc
    20461 acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat attgccctct
    20521 tcctcgatca gagagcgcaa acggcgagct atttgcaata ccacatcttt ttcgcgcgtt
    20581 ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag cataatgaca
    20641 atcggacgat cgcgccctgc tttacccggt tgtggaccac tttgcgctgg cggcacctgc
    20701 ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc
    20761 tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc gaccggcgca
    20821 agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatgcg tacggtttgc
    20881 gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtctgcacg aatttgtgcc
    20941 gccatcccct tgagtaccga gttcaggttt acatcttcga tatccaccac cacgcgttca
    21001 gggttactca acgcgaactg cttatatttc agctgacgat ttgattctac cgtcacgcgg
    21061 gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctaaccgc agccaggctg
    21121 acctgactta cgctcaatag ccacatagcg cccgcgcctt gcagtaaacg acgacggctg
    21181 attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca
    21241 agtaaacgac taatttgacc ggaaacttta gcgaaagacg cataatctgt catctaataa
    21301 acggtaaaca ttctttttat attcacggca ttactgataa aaaagtcgct ctcgcataaa
    21361 atttacactt gcaccctgcg aaaaaacaga ataaaaatac aataatttca aataatcatg
    21421 caaaggggtg tgccgtggta aaggaacgta aaaccgagct ggtcgaggga ttccgccatt
    21481 cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc ggcggtgaag
    21541 ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg cacagcctcg
    21601 gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat ctggctgcgc
    21661 atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgccaaa acactggaac
    21721 tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg tcaatgagtc
    21781 tcaataacac gccgctgcag ggcgcgcata tcaacgtcgt tagtggcaat tttattattg
    21841 cccagccgct gggcgtcgat gacggcgtgg attactgcca tagcgggcgt atccggcgga
    21901 ttgatgaaga cgcgctccat cgtcaactgg aaagcggtgc aatagtgcta atggggccgg
    21961 tcgctgtttc agtcactggc gagagcttta atctgacctc ggaagagatt gccactcaac
    22021 tagccatcaa actgaaagct gaaaagatga ttggtttttg ctcttcccag ggcgttacta
    22081 atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca cgggtagaag
    22141 cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga
    22201 aagcctgccg cagcggcgtg cgccgctgtc atttaatcag ttatcaggaa gatggcgcgc
    22261 tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg gaaagcgccg
    22321 agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg attcgcccgc
    22381 tggagcagca aggtattctg gtgcgccgtt ctcgcgagca gctggagatg gaaatcgaca
    22441 aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc tatccgttcc
    22501 cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac cgcagttcat
    22561 cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc ggcttaagca
    22621 aattgtttgt gctgaccacg cgcagtcttc actggttcca ggaacgtgga tttaccccgg
    22681 tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag cgtaaatcca
    22741 aagtgttgat ggcggattta gggtaagcga tgaaaatcac cggatgcggc aggcgtaaca
    22801 ctcgtgcgtc gcatccggca attacgttta ttcccgcgaa ctaaacaacg ccgccagacc
    22861 actgcgccgc tcagtacgag tggcgattgc cgcacttaat atacgctcat cggcatacag
    22921 cgacagacga cgacgcgcgc gggtcaccgc ggtgtatacc agctctcgcg ttactaccgg
    22981 cgtgcgttgg ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
    23041 taccgtcatc gcccacgtag tttcgtgctc tggcaagcga ctcggttgca cagacttaat
    23101 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
    23161 gatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ccggtcggcc
    23221 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaactgttc
    23281 aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc acaaaagctg
    23341 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
    23401 ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt cgccgctctg
    23461 taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
    23521 atcaccacgg ttgatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata
    23581 gcttttttgc agcaggcaga gactgtcgcg caaagatgcc gcttctgtgc cggttcctgc
    23641 cggaacgtga gtccccgtca ggcggcttag ctgcctggca cgctcggcgg taaagcccgc
    23701 gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
    23761 acgatcgccg agaaagatca ctcgcgcatg atctggcaag gcgtcgatca gtctcgacat
    23821 cataggcaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
    23881 accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt
    23941 gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaact gtcgcaaagc
    24001 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
    24061 acggcagcgt tcgccgtcgg ccatttgaat taacgctgcc agcaacttcg ccacggtggt
    24121 cgttttaccg gtgcctgggc cgccggaaat caccgagatc cgccgcgtca gcgccactgc
    24181 cgctgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
    24241 cgccagtaga gcttcatcaa cctcaatggt atgattcacc tcattgaaga agcgcgctac
    24301 cgtccgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
    24361 cgtgggttca tctcccctgc tgaccgcttg agaagccagc aagcattctt cccaattttg
    24421 tagctcaccg atttcactga cacaggtcgc caacagcgga ttcgacgcct cgttattttc
    24481 cagtcgtgaa agcggcaaac agacgtgtcc ctctccggca tcatgactta acagtgccgc
    24541 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaatt gcacatccag
    24601 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
    24661 tcctccaggg tcataccggc aaacatctca tccatcaggg caatcaaccc ggcgttgggt
    24721 cgggttgtgt aaatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagatag
    24781 ataacgccgc caaagtgaag atcatagtcg taatcagcaa tgcgatggcg cagataacga
    24841 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg catcgctgct
    24901 gccatagcct gttgggtgta agccgaactg tcttcaccca gccagttgga tttatagtcg
    24961 agcaggtaat aacgcccttc gtggcggaat accaggtcga taaagccttt taacatgccg
    25021 cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa ctggcgaatt
    25081 agtgcatcaa gctggctggc gatgagcggt tcgctaatcg gcaggtaaaa ctccatctcc
    25141 acctgtttat cgcggtcgga aagctgactc aggctaacac ccgtttcatt gagaggtgcc
    25201 tggaggacag ccgtgatcca ctcggtcaac accggttccc actgcgattc aaagccgccg
    25261 agttccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
    25321 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
    25381 gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg tggcatcaga
    25441 tcctgagcga taccgtgacc acgctgctgc aagccggagt agctggtgac acgccagtta
    25501 tcgccgggca atcgttgtag tgtcctcgca ttcagttctg cagttaaagc atcattaacc
    25561 tgccagggct ggttatcacc agtttgtgcc gtttgccagg caatatcatc atcgcataac
    25621 gcttcaatac aggtgcgaag ccctgccgca tcttgcggtt cccctttttg cagcaaacgc
    25681 ccgagcgcac tttggtggac gtcggtgtca ccttttttat cgccacgacg gcgcaccagc
    25741 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagcaaacgc
    25801 agatcttccg ccagacgttc gacctccgcg aggtcgacgc tttctggcgc agcattaaga
    25861 tccaaaactg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
    25921 ttggtaataa atggcaacca gaccagcgga tattccagcc ctttcgattt atggatcgtg
    25981 acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg
    26041 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
    26101 gttccggctt cttgaagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
    26161 cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc
    26221 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccgca
    26281 tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt
    26341 gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
    26401 aaccaaagca tttcctgcgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
    26461 acggaaggga tttccagcaa cgttaaggca tcgcgcacct gggcggcctc ctggcggctg
    26521 cgcaccagca cgctgatgtc cgaagcacgc accggacgcg cgtcgtcgcc gttcatcagc
    26581 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
    26641 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
    26701 atcgcaggct gcgtttcacc tttaaataca aaacgtaacg cctgattttt cccggctgat
    26761 ttcactggaa taaacggtat ttcgcgaaac atgaacgtgt catcagtctg gctgaaaagc
    26821 ttgttcacac tgttcaccat tccaggtgcg gagcgccagt tggtgtctaa agtgtagtgg
    26881 gcgtgaactt cactacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
    26941 atggcctgct tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
    27001 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc
    27061 gggaatcgcg tacggatcgc cgctgccaac acctcgccgc tttcgctacg cagcgcggaa
    27121 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
    27181 actgtttcgc ggatctcagc caatgcgcgg gtgatcaaca gatcgcggat cgacaatggt
    27241 tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggcc
    27301 ttcgtgcgat cttctaagaa acgctgggag aatttttcca gcgactccgg caactgataa
    27361 ctgtttgtct cttcttctgc ccaggcgctg atcttttcga tccatttagc ctgattgcta
    27421 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
    27481 gcgtcgcgcc actgctgttt taccgtatca atacgcgcca caatttgcgc gtggcgggaa
    27541 gccagcgttt catcatcggg cggcggtgct ttgataaccg gcggttcgcc ttgcagataa
    27601 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggct
    27661 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
    27721 agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc
    27781 aggttgagca tgcgctggca aaagccgtga atagtaaaga ctgccgcttc atccatctgc
    27841 cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
    27901 cgttcgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
    27961 ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt gaccaccagc
    28021 agttcttcaa cgatcagcgg gcggggaaag gcggcggaac cgcctagtcc aagtaacagg
    28081 cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
    28141 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
    28201 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
    28261 ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg acagaatagc
    28321 catgccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt gcggcgtcag
    28381 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
    28441 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
    28501 aattaccgcc tgctggattt gcgcaaactc atctggcttc atcgctcgca attttgcctc
    28561 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
    28621 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
    28681 atagcccaat tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
    28741 caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
    28801 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
    28861 gaccactaca tctttgtttc gacaccactc tgaaccatca gcgcccaact gtttttgcac
    28921 atcgcgtgcc agcgttgttg cctgggcctc ggtcatgttg ccgataacca taaactctgg
    28981 tcgagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
    29041 caaaatttta cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
    29101 cataatcgcc tgctcaaacg ctttaccctt ttctgcggaa tccatcatct ggttatacca
    29161 ggacttcgcc tgctcaagct gctcttccgt agcggtatag ctaaagtacc cctcgagcaa
    29221 tgcctggaac agctgcggca gacgctgggt gtaaccatta gcattaatca taaggccgtt
    29281 gttagcgttg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta actgatcaag
    29341 cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg cgctgtccat
    29401 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg caaaataacg
    29461 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcagctctg gatggtcgta
    29521 tttcttctct gacttaatca gcgagaaatc atcaggaata taagggttaa gctctggcaa
    29581 agagagcgca atgtcggcgg cttttttctg ccagtcggcg aaagtttgtg cgctgatttt
    29641 atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct
    29701 gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttcct ttactgcttt
    29761 agcatcgtac cgatcggcaa tattgactgc atccagcgta tgctcaacag gaacgcgaat
    29821 catggtatct gccagccatt cgacgtaatc catatcacgg gtgatcgacg gataacggaa
    29881 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcaatgc ctttttcacg
    29941 taacagattg agatagctaa aaattgccgc cacaacctga tcgcgattag ccaagccttt
    30001 atcagttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    30061 gttggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtacctgg
    30121 gctgcgattg ccaatcagat aggtaatcaa ttcatcggtt ttactacgga acttcgctga
    30181 gttgttatcg atgcgaaact caacgcgcaa cactttacgc ggcagcgcag ggacgtaatg
    30241 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    30301 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt tttgccaact ccggcagcgg
    30361 tttattactg taaataaccg ccttcatcaa attggcggaa tagtacttct cgtggaaatc
    30421 tttcagcgcc tgctgcaccg gattaccagg tttgtcgctt aaagtttcga ggttaccacc
    30481 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    30541 catcccgtca cgcgtacgcg ccatggttaa ttcagcgttc actgcattac gctcacgttc
    30601 ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt ctaccgcacc
    30661 gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggcg cagtgctggc
    30721 attgtgacta ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcgggtactt
    30781 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcggg
    30841 atcttccagc gacccaacgg gcactaccag cgccgagagc gatttaactg cctgcggatc
    30901 agaaaccagc aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    30961 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    31021 tggtgcccag agggcaacaa acaacaataa tgctttgaac caggtgctgc ggggcattca
    31081 cggacctcat aatcaacttt attttctgga cagattcaac aggttaacgc aaatcatctg
    31141 ccagaattta atcttgtgct gcacgagtca gcctatgttt atataaccat cagtccgtga
    31201 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac actcatgact
    31261 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgatg gcctccattg
    31321 tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca
    31381 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    31441 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    31501 tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg
    31561 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactca ccgtctttgc
    31621 gtagaaaaag gcgactttcg ccattaccac cgctggcaca gtagacaagg tgttccagcc
    31681 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    31741 gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga tcaatttcca
    31801 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctggc
    31861 actgtgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    31921 agcggcggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    31981 tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtctggg atttcgctgt
    32041 cttcagtatg aaagttcacc tgcaaacgca tctggaagaa cgcccgcacc ggatgtgccc
    32101 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaacggc agcggctgaa
    32161 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcatagc
    32221 tttgtcgttc gcctggctgg tagttttgcg gatcaaacgg catccgggta tggaggcaag
    32281 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    32341 gataatgact ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    32401 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    32461 ttaacgcttc caggaacagg tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    32521 gtttctggct catcaggtca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    32581 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    32641 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccaga cgctgtgcca
    32701 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    32761 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    32821 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttct agcggacgct
    32881 cctgtgccag cccgcgacgc cagatgttta gctgcattag cagtgaagcc agatgcccca
    32941 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    33001 gcgcgctctc catcgcgtag cccagcaaca tacgcgtcag gccaaatcgc caggtgtgtt
    33061 gtccggtggc ggggagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    33121 cggattcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    33181 ccgccagcac cggcacatcc agcagcgcca gcacatcctc tgacacaaag cgactgtcag
    33241 gcagtgataa caggctgata aacgcttcca gtaccggatg tgactgccgc gcacgacggt
    33301 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacact gcctgaataa
    33361 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    33421 gggcttcctc cagcatcgcc agcaagcgat cgtgtaaaac ttcaacttca cgctgcgggc
    33481 tatggcaaac gtggaatgtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    33541 tacgggaaaa ctcttcgatg ttcacacccg caacggcgcg gttttccagt tccagaatgt
    33601 cagactgaat gttatgcagc aggttatctg gcgtcacatc gacaaaagcg tccagctcct
    33661 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttc ccccatgagg
    33721 ccagcagcga gttgccgaca tcctgttcac catcgctgtt aaagagctgt ccggcatttt
    33781 cgctatcgcg aaatagcggc aattcgcgat cttcaaaact gtgtcgacgc tgacgagtca
    33841 gcagtttcgc cagataagca ggatctttaa tatcgcccca gtaataacgg caggggttgg
    33901 taaacaggag atggatttca atatgtttac ccagcgcctg gagcgcctgg agataaacag
    33961 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    34021 tcgcggactc cagcgtttcg ataaagcgct gatagagatt cgcgcggtgc cagcgcggtt
    34081 gtccgagttc atgggtatat tccaccagcg ccttccacaa cggggcttgc caggcctgtg
    34141 cttctcccag cccttcaacc aaatgtcctg tttcccactg tgccagccag tccggacgat
    34201 agaccagata ctggtcaaac aggtccgccg cttttgagga aagctggaac agttttcgct
    34261 tgtcgctatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccagcaatt
    34321 gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    34381 ggatttccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    34441 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgcgccatac
    34501 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    34561 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    34621 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    34681 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    34741 ggttgacctg ccagttggca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccaaccat
    34801 gccgccagag ctgttggtac tggtttctgc tggcgagact gttcattgat gttcgctgga
    34861 tacccgataa cgcagtgaca atcatcacca tcagcaccat cagcaccatc gcaaccatta
    34921 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    34981 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    35041 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    35101 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    35161 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    35221 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    35281 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    35341 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    35401 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    35461 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    35521 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    35581 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    35641 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    35701 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    35761 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    35821 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    35881 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    35941 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    36001 agcgaatatg cccggcccat gcggtattac gcaggccaaa gaaagcaagc gaaggtgtca
    36061 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    36121 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    36181 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    36241 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    36301 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    36361 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    36421 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    36481 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    36541 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    36601 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    36661 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    36721 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    36781 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    36841 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    36901 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    36961 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    37021 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    37081 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    37141 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    37201 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    37261 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    37321 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    37381 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    37441 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    37501 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    37561 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    37621 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    37681 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    37741 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    37801 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    37861 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    37921 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    37981 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    38041 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgtccggac
    38101 gattcgcccg acgtgttgcc agccacattg caaaaatgaa acccaccaga tacatcaggc
    38161 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    38221 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    38281 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    38341 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    38401 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    38461 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    38521 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    38581 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    38641 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    38701 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    38761 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gacccgcttt gccagatgcg
    38821 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    38881 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    38941 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    39001 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    39061 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    39121 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    39181 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    39241 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    39301 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    39361 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    39421 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    39481 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    39541 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgagccatct cgcacgacaa
    39601 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    39661 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    39721 gcaatcgctg acccagcgca cgtaaatcgc cagcccgctc tttgagatag ttatcgctta
    39781 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    39841 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    39901 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    39961 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    40021 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    40081 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    40141 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    40201 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    40261 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    40321 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    40381 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    40441 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    40501 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    40561 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    40621 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    40681 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    40741 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    40801 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg cagattgatt tctgcatcgc
    40861 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    40921 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    40981 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    41041 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    41101 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    41161 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    41221 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    41281 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    41341 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    41401 cattctgaat aagcccgata tagaaatttt aaaatattca ttattagtca atgcattaca
    41461 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    41521 ttggcgaaag atcaaccaat gccgtattta tccacagaat gtgccactaa gttaagcact
    41581 taaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    41641 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    41701 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    41761 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    41821 cggcacataa ttactgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    41881 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    41941 ttatacattg ggagaactgg cggcacttgc cgggctggtt acgccagaga atttaaaacg
    42001 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    42061 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    42121 tggtcgtccg ctggaaacaa cattcgtttg tgttgccccg ttaacgggta atagcggggt
    42181 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    42241 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    42301 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    42361 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    42421 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    42481 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    42541 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    42601 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    42661 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    42721 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    42781 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    42841 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    42901 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    42961 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    43021 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    43081 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    43141 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    43201 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    43261 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    43321 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    43381 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    43441 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    43501 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    43561 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    43621 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    43681 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    43741 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    43801 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    43861 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    43921 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    43981 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    44041 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    44101 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    44161 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    44221 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    44281 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    44341 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    44401 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    44461 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    44521 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    44581 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    44641 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgct gtcaaccgtt
    44701 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    44761 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    44821 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    44881 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    44941 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    45001 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    45061 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    45121 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    45181 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    45241 cttcgctgcc gcacctgcgc caaccacgat accaatcact accatcgcgt tgagataggt
    45301 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    45361 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    45421 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    45481 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    45541 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    45601 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    45661 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    45721 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    45781 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    45841 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    45901 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgtca gagtggcaaa
    45961 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    46021 cttcgaccac aacgaagtgt taatgtgcac tgactcactc atcgtgttgt tccgcttcgt
    46081 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    46141 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    46201 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    46261 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    46321 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    46381 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    46441 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    46501 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    46561 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    46621 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    46681 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    46741 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    46801 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    46861 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    46921 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    46981 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    47041 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    47101 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    47161 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    47221 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    47281 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    47341 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    47401 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    47461 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    47521 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    47581 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    47641 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    47701 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    47761 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    47821 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    47881 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    47941 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    48001 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    48061 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    48121 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    48181 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    48241 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    48301 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    48361 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    48421 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    48481 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    48541 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    48601 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    48661 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    48721 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    48781 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    48841 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    48901 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    48961 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    49021 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    49081 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    49141 tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt
    49201 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    49261 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    49321 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    49381 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    49441 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    49501 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    49561 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    49621 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    49681 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    49741 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    49801 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    49861 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    49921 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    49981 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    50041 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    50101 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    50161 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    50221 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    50281 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    50341 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    50401 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    50461 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    50521 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    50581 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    50641 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    50701 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    50761 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    50821 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    50881 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    50941 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    51001 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    51061 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    51121 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    51181 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    51241 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    51301 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    51361 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    51421 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    51481 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    51541 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    51601 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    51661 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    51721 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    51781 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    51841 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    51901 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    51961 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    52021 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    52081 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    52141 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    52201 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    52261 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    52321 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    52381 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    52441 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    52501 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    52561 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    52621 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    52681 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    52741 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    52801 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    52861 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    52921 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    52981 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    53041 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    53101 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    53161 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    53221 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    53281 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    53341 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    53401 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    53461 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    53521 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    53581 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    53641 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    53701 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    53761 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    53821 gctggtaaag caagaccccc caacattgcg cgccagttac cgctataact gaacgctgta
    53881 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    53941 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    54001 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    54061 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    54121 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    54181 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    54241 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    54301 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    54361 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    54421 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    54481 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    54541 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    54601 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    54661 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    54721 ccacagcaat ggcataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    54781 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    54841 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    54901 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    54961 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    55021 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    55081 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    55141 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    55201 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    55261 gcagatcggc ggtcaggctt aaaaaacgac gcccctgcgc cgtgacctgc tcgatggttt
    55321 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    55381 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    55441 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    55501 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    55561 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    55621 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    55681 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    55741 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    55801 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    55861 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    55921 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    55981 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    56041 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    56101 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    56161 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    56221 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    56281 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    56341 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    56401 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    56461 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    56521 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    56581 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    56641 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    56701 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    56761 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    56821 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    56881 agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
    56941 gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc
    57001 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
    57061 tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
    57121 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
    57181 ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
    57241 tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag
    57301 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat
    57361 gcaccagact gtcaaccaac tggctattgc caagctgtgc accggtgcgg ctatcagtca
    57421 gaacatgtgg tgcgcggctc atgttttcct ggccaccggc gatgacaata tcagcctcgc
    57481 cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat
    57541 taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg
    57601 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
    57661 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
    57721 aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
    57781 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
    57841 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
    57901 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt
    57961 agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt
    58021 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
    58081 tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag
    58141 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
    58201 tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa
    58261 gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg
    58321 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
    58381 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
    58441 ctgggtgttg tattaattct gaatctcatt tttctgatgg gacg
//
LOCUS       DAECNO010000011        58080 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.011, whole genome shotgun sequence.
ACCESSION   DAECNO010000011 DAECNO010000000
VERSION     DAECNO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.011"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            698..1126
                     /gene="uspC"
                     /locus_tag="KKO08_000697"
     CDS             698..1126
                     /gene="uspC"
                     /locus_tag="KKO08_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBD6317074.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="KKO08_000698"
                     /pseudo
     CDS             complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="KKO08_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="KKO08_000699"
     CDS             complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="KKO08_000699"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBD6317075.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3489..4475)
                     /gene="araH"
                     /locus_tag="KKO08_000700"
     CDS             complement(3489..4475)
                     /gene="araH"
                     /locus_tag="KKO08_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBD6317076.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4490..6004)
                     /gene="araG"
                     /locus_tag="KKO08_000701"
     CDS             complement(4490..6004)
                     /gene="araG"
                     /locus_tag="KKO08_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBD6317077.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6074..7063)
                     /gene="araF"
                     /locus_tag="KKO08_000702"
                     /pseudo
     CDS             complement(6074..7063)
                     /gene="araF"
                     /locus_tag="KKO08_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            7860..8363
                     /locus_tag="KKO08_000703"
     CDS             7860..8363
                     /locus_tag="KKO08_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBD6317078.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8443..8694)
                     /locus_tag="KKO08_000704"
     CDS             complement(8443..8694)
                     /locus_tag="KKO08_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBD6317079.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="KKO08_000705"
     CDS             complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="KKO08_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBD6317080.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9158..9481
                     /locus_tag="KKO08_000706"
     CDS             9158..9481
                     /locus_tag="KKO08_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBD6317081.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9652..10149
                     /gene="ftnA"
                     /locus_tag="KKO08_000707"
     CDS             9652..10149
                     /gene="ftnA"
                     /locus_tag="KKO08_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBD6317082.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10186..10425)
                     /locus_tag="KKO08_000708"
     CDS             complement(10186..10425)
                     /locus_tag="KKO08_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBD6317083.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10615..11826
                     /gene="tyrP"
                     /locus_tag="KKO08_000709"
     CDS             10615..11826
                     /gene="tyrP"
                     /locus_tag="KKO08_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBD6317084.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="KKO08_000710"
     CDS             complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="KKO08_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBD6317085.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12749..12835)
                     /locus_tag="KKO08_000711"
     tRNA            complement(12749..12835)
                     /locus_tag="KKO08_000711"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12799..12801),aa:Leu,seq:taa)
     gene            complement(12848..12921)
                     /locus_tag="KKO08_000712"
     tRNA            complement(12848..12921)
                     /locus_tag="KKO08_000712"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12887..12889),aa:Cys,seq:gca)
     gene            complement(12976..13051)
                     /locus_tag="KKO08_000713"
     tRNA            complement(12976..13051)
                     /locus_tag="KKO08_000713"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13016..13018),aa:Gly,seq:gcc)
     gene            complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="KKO08_000714"
     CDS             complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="KKO08_000714"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBD6317086.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="KKO08_000715"
     CDS             complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="KKO08_000715"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBD6317087.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="KKO08_000716"
     CDS             complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="KKO08_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBD6317088.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16589..16765
                     /locus_tag="KKO08_000717"
     CDS             16589..16765
                     /locus_tag="KKO08_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317089.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16752..16976
                     /locus_tag="KKO08_000718"
     CDS             16752..16976
                     /locus_tag="KKO08_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBD6317090.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="KKO08_000719"
                     /pseudo
     CDS             complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="KKO08_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="KKO08_000720"
     CDS             complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="KKO08_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBD6317091.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="KKO08_000721"
     CDS             complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="KKO08_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBD6317092.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="KKO08_000722"
     CDS             complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="KKO08_000722"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBD6317093.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="KKO08_000723"
     CDS             complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="KKO08_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6317094.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="KKO08_000724"
     CDS             complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="KKO08_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBD6317095.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="KKO08_000725"
     CDS             complement(21867..22586)
                     /gene="fliA"
                     /locus_tag="KKO08_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBD6317096.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="KKO08_000726"
     CDS             complement(22751..24403)
                     /gene="fliC"
                     /locus_tag="KKO08_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBD6317097.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24669..26075
                     /gene="fliD"
                     /locus_tag="KKO08_000727"
     CDS             24669..26075
                     /gene="fliD"
                     /locus_tag="KKO08_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBD6317098.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26100..26510
                     /gene="fliS"
                     /locus_tag="KKO08_000728"
     CDS             26100..26510
                     /gene="fliS"
                     /locus_tag="KKO08_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBD6317099.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26510..26875
                     /gene="fliT"
                     /locus_tag="KKO08_000729"
     CDS             26510..26875
                     /gene="fliT"
                     /locus_tag="KKO08_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBD6317100.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26953..28440
                     /gene="amyA"
                     /locus_tag="KKO08_000730"
     CDS             26953..28440
                     /gene="amyA"
                     /locus_tag="KKO08_000730"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBD6317101.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28474..28887)
                     /locus_tag="KKO08_000731"
     CDS             complement(28474..28887)
                     /locus_tag="KKO08_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6317102.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29074..30279
                     /gene="yedE"
                     /locus_tag="KKO08_000732"
     CDS             29074..30279
                     /gene="yedE"
                     /locus_tag="KKO08_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBD6317103.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30276..30509
                     /gene="yedF"
                     /locus_tag="KKO08_000733"
     CDS             30276..30509
                     /gene="yedF"
                     /locus_tag="KKO08_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBD6317104.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30616..31286
                     /locus_tag="KKO08_000734"
                     /pseudo
     CDS             30616..31286
                     /locus_tag="KKO08_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31326..32534)
                     /locus_tag="KKO08_000735"
                     /pseudo
     CDS             complement(31326..32534)
                     /locus_tag="KKO08_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            32602..33217
                     /gene="tnpA"
                     /locus_tag="KKO08_000736"
                     /pseudo
     CDS             32602..33217
                     /gene="tnpA"
                     /locus_tag="KKO08_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            33545..34627
                     /locus_tag="KKO08_000737"
     CDS             33545..34627
                     /locus_tag="KKO08_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBD6317105.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(35116..35914)
                     /locus_tag="KKO08_000738"
                     /pseudo
     CDS             complement(35116..35914)
                     /locus_tag="KKO08_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(35924..36475)
                     /gene="ybcL"
                     /locus_tag="KKO08_000739"
     CDS             complement(35924..36475)
                     /gene="ybcL"
                     /locus_tag="KKO08_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBD6317106.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(36644..36976)
                     /gene="emrE"
                     /locus_tag="KKO08_000740"
     CDS             complement(36644..36976)
                     /gene="emrE"
                     /locus_tag="KKO08_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBD6317107.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(37321..37635)
                     /gene="fliE"
                     /locus_tag="KKO08_000741"
     CDS             complement(37321..37635)
                     /gene="fliE"
                     /locus_tag="KKO08_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBD6317108.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            37849..39514
                     /gene="fliF"
                     /locus_tag="KKO08_000742"
                     /pseudo
     CDS             37849..39514
                     /gene="fliF"
                     /locus_tag="KKO08_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            39507..40502
                     /gene="fliG"
                     /locus_tag="KKO08_000743"
     CDS             39507..40502
                     /gene="fliG"
                     /locus_tag="KKO08_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBD6317109.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            40495..41181
                     /gene="fliH"
                     /locus_tag="KKO08_000744"
     CDS             40495..41181
                     /gene="fliH"
                     /locus_tag="KKO08_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBD6317110.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            41181..42554
                     /gene="fliI"
                     /locus_tag="KKO08_000745"
     CDS             41181..42554
                     /gene="fliI"
                     /locus_tag="KKO08_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBD6317111.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            42576..42917
                     /gene="fliJ"
                     /locus_tag="KKO08_000746"
                     /pseudo
     CDS             42576..42917
                     /gene="fliJ"
                     /locus_tag="KKO08_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            42914..44041
                     /gene="fliK"
                     /locus_tag="KKO08_000747"
     CDS             42914..44041
                     /gene="fliK"
                     /locus_tag="KKO08_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBD6317112.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            44146..44610
                     /gene="fliL"
                     /locus_tag="KKO08_000748"
     CDS             44146..44610
                     /gene="fliL"
                     /locus_tag="KKO08_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBD6317113.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            44615..45619
                     /gene="fliM"
                     /locus_tag="KKO08_000749"
     CDS             44615..45619
                     /gene="fliM"
                     /locus_tag="KKO08_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBD6317114.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            45616..46029
                     /gene="fliN"
                     /locus_tag="KKO08_000750"
     CDS             45616..46029
                     /gene="fliN"
                     /locus_tag="KKO08_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBD6317115.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            46032..46397
                     /gene="fliO"
                     /locus_tag="KKO08_000751"
     CDS             46032..46397
                     /gene="fliO"
                     /locus_tag="KKO08_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBD6317116.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            46397..47134
                     /gene="fliP"
                     /locus_tag="KKO08_000752"
     CDS             46397..47134
                     /gene="fliP"
                     /locus_tag="KKO08_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBD6317117.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            47144..47413
                     /gene="fliQ"
                     /locus_tag="KKO08_000753"
     CDS             47144..47413
                     /gene="fliQ"
                     /locus_tag="KKO08_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBD6317118.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            47422..48207
                     /gene="fliR"
                     /locus_tag="KKO08_000754"
     CDS             47422..48207
                     /gene="fliR"
                     /locus_tag="KKO08_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBD6317119.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            48492..49115
                     /gene="rcsA"
                     /locus_tag="KKO08_000755"
     CDS             48492..49115
                     /gene="rcsA"
                     /locus_tag="KKO08_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBD6317120.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(49159..49347)
                     /gene="dsrB"
                     /locus_tag="KKO08_000756"
     CDS             complement(49159..49347)
                     /gene="dsrB"
                     /locus_tag="KKO08_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBD6317121.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            49510..49737
                     /gene="yodD"
                     /locus_tag="KKO08_000757"
     CDS             49510..49737
                     /gene="yodD"
                     /locus_tag="KKO08_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBD6317122.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            50035..50850
                     /locus_tag="KKO08_000758"
                     /pseudo
     CDS             50035..50850
                     /locus_tag="KKO08_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(50847..52541)
                     /gene="yedQ"
                     /locus_tag="KKO08_000759"
                     /pseudo
     CDS             complement(50847..52541)
                     /gene="yedQ"
                     /locus_tag="KKO08_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(52712..52894)
                     /locus_tag="KKO08_000760"
     CDS             complement(52712..52894)
                     /locus_tag="KKO08_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBD6317123.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(52973..53890)
                     /locus_tag="KKO08_000761"
     CDS             complement(52973..53890)
                     /locus_tag="KKO08_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBD6317124.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            54063..54983
                     /gene="yedA"
                     /locus_tag="KKO08_000762"
     CDS             54063..54983
                     /gene="yedA"
                     /locus_tag="KKO08_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBD6317125.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(54972..55442)
                     /gene="vsr"
                     /locus_tag="KKO08_000763"
     CDS             complement(54972..55442)
                     /gene="vsr"
                     /locus_tag="KKO08_000763"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBD6317126.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(55423..56841)
                     /gene="dcm"
                     /locus_tag="KKO08_000764"
     CDS             complement(55423..56841)
                     /gene="dcm"
                     /locus_tag="KKO08_000764"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBD6317127.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(56908..57448)
                     /locus_tag="KKO08_000765"
                     /pseudo
     CDS             complement(56908..57448)
                     /locus_tag="KKO08_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(57488..57745)
                     /locus_tag="KKO08_000766"
     CDS             complement(57488..57745)
                     /locus_tag="KKO08_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317128.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            complement(57746..>58080)
                     /locus_tag="KKO08_000767"
     CDS             complement(57746..>58080)
                     /locus_tag="KKO08_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBD6317129.1"
                     /translation="RNPEKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDN
                     AVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQ
                     YYQRLGSV"
BASE COUNT        14601 a        14866 c        13968 g        14645 t
ORIGIN      
        1 ttagcattag aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa
       61 gtccttacaa atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
      121 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
      181 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
      241 tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
      301 gaataatcga aatgggatgt tgcgcacggt taaaataact caccgtaaat aatcatctgc
      361 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
      421 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat aaaatgtttt
      481 tcatatatta accacttgtt tgatgcgggg aatcagtaca ttattttctt ataattacag
      541 ttgaaatcat atgatcaccg agagtgattg attaattgtc tttactaata atcagactaa
      601 tatttacctg tttgactgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
      661 tgtcacctga agttcacagc agaataagga acaggttatg agctatagca atattcttgt
      721 cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag
      781 gccagtaaag ggacacatca gtttaattac tctcgcttcc aacccggaaa tgtacaatca
      841 attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt
      901 tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg
      961 tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcaatt tggtgatttg
     1021 cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc
     1081 ctcaagtgag gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa
     1141 gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct
     1201 gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt
     1261 ccagcatttc tgcatgacgg gaaatacgtt ccaccagcga catagtcaat gcacgatcca
     1321 gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt
     1381 ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag
     1441 caacatactc ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt
     1501 cagagtagcg gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa
     1561 gcggcgtcca gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct
     1621 gatgacgaat atcctggtag gcttgcacat caccacgcga cgttggtgca atctgggtat
     1681 aacgaatttt accatgatgc tgcggatatt tttccagcaa tgcctcatag gcgagaaaac
     1741 gctccggcaa acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt
     1801 tcaattccgc tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta
     1861 tttctttcgg ttcaatgccg atcgggtaga cttctgttcg aaatgctttt ccccaggctg
     1921 tatggctttt tgcgctacgt gtcgtgacgc gggtcaggtt agaaaggcaa tccaggaacg
     1981 ccagacgatc gttttctgtc tgtaaaccca gcaaatcata atcacaaagc tgttcaagca
     2041 aggtgtcata tgtcggcagc gcgttgaaaa tttccggtgt cgggaaagga atatgcaaga
     2101 aaaaaccaat gcgattattc accccacgtt tgcgtaattc atgcgcaaag ggcaacaggt
     2161 gataatcgtg gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca
     2221 gcaacgcatt tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga
     2281 gccgataatg aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa
     2341 ggtcctgttc gctgaggtta aaagaggccc acgtaatgtt acctttttta gcggctgatc
     2401 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
     2461 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
     2521 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
     2581 gtaatgcggc ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
     2641 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
     2701 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
     2761 aaggagcttc ctgcataaag gctgcaatcg cttcaccttt actggtacct ctcggtttga
     2821 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
     2881 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
     2941 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgaccgta tgcagttgca
     3001 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
     3061 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
     3121 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
     3181 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
     3241 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
     3301 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gtgttgcgca
     3361 gaccgcgctt tacagacatg taagtataga cagtgtgacg gaggtcgcca tttgcaacga
     3421 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
     3481 aaaaagcatc agacagtgcg cttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
     3541 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
     3601 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
     3661 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
     3721 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
     3781 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tatacccgcc
     3841 agacgcgcag cctcttcgtt cccgccaatc gccagggtat tacgaccaaa ggtggtttta
     3901 ttcagcagca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
     3961 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
     4021 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgtcgtgatc
     4081 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaacgccc
     4141 agcaacaaac ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
     4201 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
     4261 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
     4321 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
     4381 gcaaacacca ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta
     4441 ggtgcgccag accccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
     4501 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
     4561 acaattcacc ggcgatttcg ccttcccgca tcaccacaat ccggtcggca acgccgagga
     4621 cttcaggtaa gtcgctggag gcaaacagca ccgccacgcc ctgcgccgcc agcgcataaa
     4681 ttacgttata tatttcgtgc ttagcgccaa catcaatgcc gcgcgtaggt tcatccagca
     4741 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
     4801 ctgagagatt catgatcagt tgctccgcgc ccggcgtttt gatgttgagc gaacgaatgt
     4861 gctgatcggc attgttttct tcccaactgt tgttgattac acaaccgccg agcacatgtt
     4921 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
     4981 ctttgcgagc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
     5041 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
     5101 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
     5161 cactgcgaac cgccagactt attggcgtac gcacgcctag tgctttcaca gcatcaagac
     5221 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
     5281 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
     5341 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
     5401 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
     5461 gattgccgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
     5521 cgttacgcgc cagcgctttg gcgatttcaa ccatctgcca ctgaccaatg gcgagatatt
     5581 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
     5641 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
     5701 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
     5761 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
     5821 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
     5881 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
     5941 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
     6001 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
     6061 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
     6121 cacgcgtgat cagtaccacg tctgtaactt cggtaaattt tggcgtttca acgtcttttg
     6181 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
     6241 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gcttactgca tccacaccgt
     6301 taataccaat accgatgata tcggctgctt taaagccctg accttcggtt gcacgtacgc
     6361 cgccaagtac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
     6421 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
     6481 cctgataaat ttgtttttcc gggaatcctg cagctttcag tgcgtccata gaacccgttg
     6541 tacggcggcg ggcggtatcc agttcgttgg cggtaatcgc catcactgcg ctttctttga
     6601 catcccagcc acgtttctgc atctctttat acagttccta gccctgacgt tcgccaattt
     6661 tagtcgccgc catcatcacc agaggaacgg tatccattgg cttacctttg gcgttaacaa
     6721 actggtcatc cactgcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     6781 agccgagttt cgggtccgga gtacaaataa cgaaaccttt tgcgccactg gctgccaggc
     6841 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     6901 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     6961 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     7021 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     7081 gtgtcgtttt gtgtagtgca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     7141 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     7201 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     7261 ggaaaagaca taaggaaaag ccaatttgtc agacaaattg ttgaatgcac agcagattaa
     7321 tccataagat tagcctggaa atccttgttg tcttcggtac ccattcggga tgtcttcttt
     7381 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccac
     7441 aactcaaaga tgtggaaaat gcgcgtcatt catttcgcca ttaattatca ctgtgctcat
     7501 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     7561 atcatcactt catcatgaat tcaaattcat cgattaatat caacaagata caaaaagcac
     7621 tatcattaaa attcattgca tttacattga tttcaccaat gaaatgtaaa aatatataaa
     7681 cttgatgatt taagcatttt cttataaccg ttcagacgtt attcttattt cagatcatcg
     7741 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
     7801 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
     7861 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
     7921 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
     7981 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
     8041 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttccaggt gaaaaactga
     8101 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcactc
     8161 tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
     8221 gcgatctgga aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag
     8281 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
     8341 tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa tacattgcgc cgatgaagtt
     8401 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
     8461 tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg
     8521 gtctgcggca gcgggatgat gagtaaagtc gatattcttc agctgactgg acattttttc
     8581 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     8641 gcaagctacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     8701 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     8761 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     8821 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     8881 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     8941 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     9001 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     9061 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc
     9121 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     9181 ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga
     9241 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     9301 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     9361 cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     9421 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     9481 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     9541 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     9601 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     9661 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     9721 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     9781 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     9841 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     9901 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     9961 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat
    10021 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
    10081 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
    10141 caaaactaat gctctccggc ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat
    10201 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
    10261 aaacgctacc agctctcccg ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg
    10321 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
    10381 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
    10441 aaagcctagc gtagcgatta ccgcttatga agactttgcg ccagcgcagg actgaatgct
    10501 tttattgtac atttatattt acacaatatg taacgtcggt ttgacgaagc agccgttatg
    10561 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
    10621 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
    10681 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
    10741 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
    10801 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
    10861 acgggcttca gtatgatgtt cttaatgtat gctctgactg cagcatacat cagcggtgcc
    10921 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
    10981 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc
    11041 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
    11101 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
    11161 gcactgtctg caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt
    11221 attgtcagct atatggatgg caacattcgt aagctacgct gggtgtttat aatcggtagt
    11281 gccatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
    11341 acaaccttta tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta
    11401 cgcgaaatgg tggcctctcc gcatgttgag ctggcggtgc atttatttgc tgatttagcc
    11461 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt
    11521 cagcgttcaa ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg
    11581 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
    11641 gcgctggcgg tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac
    11701 aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
    11761 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg caggcttgtt accagaagtg
    11821 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatg
    11881 tgacctctta atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat
    11941 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
    12001 gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt
    12061 caaacgcttc tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg
    12121 caatcgcctc cagcgctggt tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca
    12181 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
    12241 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
    12301 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccaac
    12361 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgttctggt tcaatctctt
    12421 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
    12481 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
    12541 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
    12601 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
    12661 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
    12721 tactttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
    12781 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
    12841 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
    12901 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc
    12961 cgatgtcttt taaactggag cgggaaacga gactcgaact cgagaccccg accttggcaa
    13021 ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
    13081 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
    13141 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac
    13201 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
    13261 gtcagtaccg cagccacaaa gaaaagtgca ataccgacgt actcaaccca aatgttcgga
    13321 cgccacagca gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag
    13381 gagacggcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
    13441 ataatttcac gggcgatcat cgttgccgcc ggtaatgtca cccaccagct gtgataatgc
    13501 tcggttacca gcaccatggc gatagccacg agaactttat ctgccacagg gtcaaggaaa
    13561 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
    13621 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
    13681 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
    13741 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
    13801 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
    13861 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
    13921 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
    13981 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc
    14041 gaatatgctg gataacatgc agcgcgggtg aatctggcgg caaactaaat ccctcacctt
    14101 ccggctcaaa gaacagcgtt tccagtccag ccttacgatc tgctcctttg gcaacgccaa
    14161 gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct
    14221 gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
    14281 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
    14341 tgatgcctgt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
    14401 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
    14461 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
    14521 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
    14581 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
    14641 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
    14701 gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt tctaccacct
    14761 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
    14821 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
    14881 cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct
    14941 gaatttggtc gcgaatacat gccgcttctt caaactccag attctggctg gcagtttcca
    15001 tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
    15061 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac
    15121 agcgccctat ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc
    15181 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
    15241 tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc atcgccagac
    15301 gcgggtgggt atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa
    15361 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
    15421 ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
    15481 gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
    15541 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
    15601 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
    15661 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
    15721 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
    15781 tgagatctca ttgaccttct ggcccttagt gatcatcagc ataatctgca attcacgttc
    15841 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
    15901 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
    15961 gacttcctgc ggagccgcgc ctttgctgag gtagccagca gcacctgcct gcatgacttt
    16021 cgctggtaaa gggttttctg tatggaccgt aagcatgatg attttaacat cagctgtgga
    16081 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
    16141 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
    16201 tccggcgact tttataccct taatatcttc cagaatgcgt cgtatccctg cgcgcaccag
    16261 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
    16321 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
    16381 aatggtgggt attcgatttt tctctcgttt ttgttaattc gattgtccac agttatggtc
    16441 acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac
    16501 aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
    16561 acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
    16621 cgtattttat agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa
    16681 gcttatggta tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac
    16741 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
    16801 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
    16861 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
    16921 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
    16981 aaaccggctg agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg
    17041 atgtcttccc ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct
    17101 ctggcgtcat cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca
    17161 gtaactgcat ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac
    17221 gggaaaagga caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc
    17281 ggcgtaaacc atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat
    17341 cattccacat taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga
    17401 gaaaattttt tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca
    17461 ctttaggtcg cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca
    17521 gctgctgagc ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa
    17581 aacgcaacag catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca
    17641 acgcccctga gagtgagtat catttataat gataattcaa actatcacat aaataagatt
    17701 tatatataat ttatattatt caggcaatga attacttttg caagccatcg cattctctta
    17761 tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc
    17821 gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aagcttctcg
    17881 aggaactggc gggtgcgagg ctgctgggga tcggcaaata acgcttttgc tgccccctgc
    17941 tcgactatcc gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc
    18001 atttcgtgtg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc
    18061 aggacttcgc ccaccagctc tggatccagc gccgacgttg gctcgtcaaa caaaatcacc
    18121 tcaggacgca ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa
    18181 cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg
    18241 cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt
    18301 tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga
    18361 cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata
    18421 tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct acgtagcaat
    18481 gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg
    18541 aggtcgatac cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt cttaacttca
    18601 atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca
    18661 acgtcgataa cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga
    18721 acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga
    18781 ttgtcgcggc cagcgacgta tcttttacca gactgataaa gctgttcgac agcggcggca
    18841 acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac
    18901 caatactggc agcggcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagcg
    18961 tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat
    19021 ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg
    19081 gcgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg
    19141 acaggcgcat cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg ccgccgatac
    19201 tgagttgcag ggtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt
    19261 gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc
    19321 aggcgcgccg ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact
    19381 gataccgtca atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc
    19441 ttccagacgc gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc
    19501 aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt
    19561 cgcaatcgcc tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac
    19621 ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc
    19681 gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca
    19741 ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc
    19801 gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc
    19861 cagttgggca ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc
    19921 caacaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc
    19981 cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg
    20041 gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc
    20101 cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatggggg tgacctcatc
    20161 ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg
    20221 cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac
    20281 agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa
    20341 aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca
    20401 ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca
    20461 tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga
    20521 cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc
    20581 gccgccagac gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg
    20641 gtcgggtcat catcataggt acgcacatcg acaccctgaa cattctgccg cagccactct
    20701 tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta
    20761 atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta
    20821 gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca
    20881 atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc
    20941 tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg
    21001 ccgtcatccc cctgaaaact gaacggcgga taagtgcctt ccagccctac cagcagcgtg
    21061 ccgctctctt tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc
    21121 gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc
    21181 atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca
    21241 aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg caggctggag
    21301 gatttctgca caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaatgcg
    21361 atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa
    21421 ccgtcctgca gctgatcgag ggaaatattt tgctcatgta gatgattacc caaacggcgc
    21481 agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat
    21541 ttcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa
    21601 aatcggcaca atgccgccca tgattgttgt tccgtttgcc agcctttttc atcaagcagc
    21661 gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg
    21721 cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga
    21781 tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca
    21841 aagttaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta
    21901 atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc
    21961 tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc
    22021 aacgtttcga tggcttccat cacccgctgg cgcagattac tgtccagcag ttgttgtagc
    22081 gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc
    22141 cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcagcatttg acgataatcg
    22201 gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc
    22261 tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc
    22321 acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa
    22381 gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cagcattaag taacccaatg
    22441 ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc
    22501 aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca
    22561 ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga gttatcggca
    22621 tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat
    22681 ttgggggtta cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc
    22741 attcagacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttggcttttg
    22801 ccagcacgga gttaccggcc tgctggataa tctgcgcttt cgacatgttg gacacttcgg
    22861 tcgcatagtc ggcgtcctga atacgggact gcgcttcaga caggttggta gtggtgttgt
    22921 tcaggttggt gactgcggaa tccaaacggt tttgcaccgc acccagggag gagcggaatt
    22981 tgtcgatgga gctgatggct tcatccagag ctttcagggg atcggctgtc gtagctgctt
    23041 ttgtttcagc atctgtcgtc aatttaccat cagcatcttt ataaactgcc ttaccgctgc
    23101 cgttagtcac tgaaccatca tcctgtaggt aaaaatctac agtatcagat gtggtcaacg
    23161 cctcgttgtt atcaacataa accgaatcaa caccttttgt tacagcgcca gttgtgccgt
    23221 tgacgctata acctgcagta gtgaaatcaa cattgtttcc cgaggttgat acagctttat
    23281 cgaaagctgc ctgatcaact tttgcacctg ttactgaata agtaatagtg tccggcgtag
    23341 tactattacc ggtaaaggag atagaggtac cgccaatgtc aatggttgtc gctttggtat
    23401 tattggctgc aagtgtggac agactagcct cagtcaaaga agcaccagag ccgtttttcg
    23461 ttaagcgccc agttgtatca atgtagagtt tatctccatt gcttgccgta attttaccat
    23521 cactgctgat ctgaacatcc tgtgcagaac catcgatagt aatagtgccc ttagcggtat
    23581 caccaactcc cggggtcaaa tatttctgaa catcggcagc tgaagccgtt gtggtatcaa
    23641 aagagtatga cttacttgca ctgtcatact tgtaattcgt tgcactagcc gccgttccga
    23701 agccgttatt aatggttgcc tgaacagtat caccatcact aactccagcc agcaaatcag
    23761 acgctttagc agcatcgtaa cccgcactca cagtatattt gttgccgacc actgttgcat
    23821 tagcagctac caagtcagct ttagatgcag cagtattagc cacagccccg ccaccattca
    23881 cattaaaccc attcagcccc agcgtatcag agtcaatttt cttcagatca atagtgatag
    23941 tctggccgtc attcgcacca acctgaattt tcatcgaacc gtcttttgcc agtacgttca
    24001 cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg gatttgattt
    24061 cgtcctgaat ggagtccaga tccgaatcag agttagtccc ggtagaagcc tgaaccgtca
    24121 gctcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg
    24181 caacagaaat accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag
    24241 taaaacggtt agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc
    24301 cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt
    24361 gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat
    24421 tgcaagtcgt tgattacgtt ttgggtttct acccgtcggc tcaatcgccg tcaaccctgt
    24481 tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt
    24541 cgacgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta
    24601 aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag
    24661 gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa
    24721 tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat
    24781 cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc
    24841 agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc accaccagca
    24901 gcaccaccgc gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa tacaccatca
    24961 gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga
    25021 aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcgac gacaaagatc
    25081 cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt gatgccatca
    25141 acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc
    25201 tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg
    25261 atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg
    25321 tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc gagaacagca
    25381 gcaacactat cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg
    25441 gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact
    25501 gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg
    25561 tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc
    25621 tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct
    25681 ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg
    25741 atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg
    25801 ttggcgatgg taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc
    25861 tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt
    25921 taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag
    25981 aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa
    26041 cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca
    26101 tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc accgtaatga
    26161 gcgccagcca acagcagttg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    26221 gagcgcgact gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag
    26281 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat
    26341 taacccaaaa cttaattgct ctttatagct atatggtcag gcgtttgctg caagccaatt
    26401 tacgcaacga tgtctccgca gtcgaagagg tggaagcatt aatgcgtaat attgccgatg
    26461 tctggaaaga gtctttactc tccccttctt cgattcagga cccagtctga tgaacaatgc
    26521 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    26581 ggcaacggaa gaacaatggg acgaactcat cgctagcgaa atggcgtatg tgaatgcggt
    26641 gcaggagatc gcacacctga ctgaagaggt tgccccgtcc actacgatgc aggagcagct
    26701 ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat
    26761 tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag
    26821 tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt
    26881 aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatat tccccttgaa
    26941 tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa
    27001 ggcggtaagc tctggcctga actggccgag cgcgcagacg gttttaatga tattggtatc
    27061 aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc ggtcggctac
    27121 gactcctatg atttatttga tctaggtgag tttgatcaga aaggcagcat ccctactaaa
    27181 tatggtgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa tgacattgcg
    27241 gtgctgctgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc
    27301 gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa
    27361 ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat
    27421 ttcaaatgtt ttagcggtat cgaccatatc gaacaccctg atgaagatgg catttttaaa
    27481 attgttaacg actataccgg agaaggctgg aacgatcagg ttgatgatga actcggtaat
    27541 ttcgattatc tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt
    27601 aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggctttcg tcttgatgcg
    27661 gtcaaacata ttccggcatg gttttataaa gagtggatcg aacacgtaca ggaagttgcg
    27721 ccaaagccgc tgtttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg
    27781 tatattgatc aggtggaagg caaaaccatg ctgtttgatg cgccgctgca gatgaaattc
    27841 catgaagcat cgcgcatggg tcgcgactac gacatgacgc agatcttcac tgggacgctg
    27901 gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac ccaaccgttg
    27961 caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg
    28021 ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc tctacggtgc acattacgaa
    28081 gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat
    28141 gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacattatt tttcgaccat
    28201 ccgaactgca ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtc
    28261 atgtcgaacg gagatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa
    28321 acctggcgtg attttctggg gaaccggcaa gagagcgtag tgacagacga aaacggcgaa
    28381 gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa
    28441 atccatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc
    28501 tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc
    28561 atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac
    28621 gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatcc cccgctgcca
    28681 ctgacggcaa tccaaggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg
    28741 actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc
    28801 gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag
    28861 cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt
    28921 ggcattaaag ctctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta
    28981 ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcggctctt
    29041 gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc
    29101 tggttgatta aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact
    29161 tactattttg gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc
    29221 ggtcagctcc tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc
    29281 catctggaag gatcgccatt aacccgcatc gacggcatga tgatcctcgg tatgtttggc
    29341 ggctgctttg ctgctgcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt
    29401 atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca
    29461 atgggctgta acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg
    29521 ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc
    29581 atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa
    29641 ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc
    29701 tgggcgctgc tcacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc
    29761 ggctttggtt tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg
    29821 tggatcaccg gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc
    29881 atcgggatct tcagttacgt acagttaggc gttgaaccta aaatcatgtg ggcgggacca
    29941 aatgcggtaa ttggtggttt actttttggt tttggcatcg tgctggcagg cggctgcgaa
    30001 accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc
    30061 aacgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc
    30121 accgactggg acaaaatcaa cctgctgaaa acttttggtc cgatgggagg cctgctggtg
    30181 acatatttgc tgctgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc
    30241 ccccgtgagg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc
    30301 gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc
    30361 cgcaattaaa aaaaggtgag atcctggaag tggtgagcga ctgtccgcag tcgatcaata
    30421 atattccact ggatgcgcgt aatcacggct acacggtgct ggatattcag caagacgggc
    30481 cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga
    30541 acatcgttac ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga
    30601 tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga ggattacctt
    30661 gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga
    30721 tacaatgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat
    30781 ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac
    30841 gcccgcgtag aaactgcctc aaccagtcgc atgtttaaac cgctctggca acatggtcgg
    30901 gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt
    30961 attttatcta tcgcgctgac ggacaacctg tttttatggc cgcgataggt agcataccgt
    31021 ttgagcgtgg tgacgaagcc gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc
    31081 tggtagatat tcatgaccga cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga
    31141 tgcggcaaga cattggtggt aaagaagcct cagaaatcgc taccagaagc tgtgtgccag
    31201 caaaccagtt cacctggcac cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg
    31261 cggagttaat tcaacctgtt tgttgacatc ctccacgccc tgaatgacga ggacccctgc
    31321 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacga gcggcctgac
    31381 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagctcca
    31441 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg
    31501 cgattttctt tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgctggcgga
    31561 acagcaagca cctgaccgcc acgccagagc tgcttgtact caagctggcg gcgcatttca
    31621 taccagccct gatccagtat cgaacggttt aaacctgatt ttgcccggac attgcgcccc
    31681 ggctggctga ccgtacccgc tgctgacttt gacatgttgc tgaccttcaa atcctcaatg
    31741 actatcatta cgtggttttt gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg
    31801 atatttgcga tacaggaatg cagtcgctgt attttgcgtt tctgcttctg ccagttgttg
    31861 ctgaatttga ccttgcggct taactgtcgc tgaagtctcg ccagcttctt ctggtttttc
    31921 tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg
    31981 ccagcatcca gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct
    32041 gcctgaatac tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaaca
    32101 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg
    32161 ctgttttcct gagcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc
    32221 tttttgaatc aggggaacgc cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg
    32281 tctttcagtg actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg
    32341 gcatttttcc actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg
    32401 gccttatgat tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct
    32461 gcgaagcgcc tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc
    32521 tgtagtcgtt tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc
    32581 ggaaggcact gtgttttcct gatgcatgct caccgggtct ttgttaccaa gtaccgacgc
    32641 cagatttttg accatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct
    32701 tattttgaag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tctgttaatt
    32761 aactatcccc ccaaactggc gatatccagt ctggtaaaca aacagcctca aaggcgtatc
    32821 gggtaggtta ctgcgacggg atcgaccaga tattgctgcc aggtattacc gcaaagatgt
    32881 tttgtggcgt cccggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg
    32941 ccaatacatt gaacaacagc aaacacctgg tcagatgaaa aaacgagcct tatatccccg
    33001 ccctgaagga cgggctttta cggtgcactg gataagcctg gagattatct acacctgcat
    33061 ggacatcatt gtactatgcc ccgtacatgc cccatcacat cctcaacatc aaagtattag
    33121 agccttgcaa accattatgc gtctcagttt gtctcatcta tcttacgcaa aaaaaataga
    33181 actaatatca agcggcgcta tattgcgtat taaataaaac cacctcaatt acattaataa
    33241 atctaaataa atatcatgca aataatttcc atggttattt ttaggctatt taaattaaaa
    33301 aaataaatta tgagtcaatc ataaataaca acctttaata taatttgact accaaattta
    33361 tattcgttca caaaatagtc atgcattgtg tgcaatagaa acagttcaga taaaaatagg
    33421 gatctacttc acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat
    33481 tggatgaata tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct
    33541 cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat
    33601 gtctgctcag gcagcagaga tttacaataa agacagtaac aagctggatc tgtacggaaa
    33661 agttaatgct aagcactact tctcttctaa cgatgcagat gatggcgaca caacttatgt
    33721 tcgtctgggc ttcaaaggcg aaactcaaat caacgatcaa ctgactggtt tcggtcagtg
    33781 ggaatatgaa ttcaaaggca accgtgctga atctcaaggt tcttccaaag ataaaacccg
    33841 tcttgccttc gctggcctga aattcggtga ctacggctcc atcgattatg gccgtaacta
    33901 cggtgtagca tacgatatcg gtgcatggac cgacgttctg ccagaattcg gtggcgatac
    33961 ctggacccaa acagatgtgt tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa
    34021 caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa
    34081 tgaccgcact gacgtaactg aagctaacgg tgatggtttc ggtttctcca ctacttatga
    34141 gtatgaagga ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta atgatcaggt
    34201 tatctacggt aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg
    34261 tctgaaatat gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat
    34321 gactgttttt ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca
    34381 atatcagttc gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga
    34441 cttgggtgcg tggggtgatc aggacctgat tgaatatatt gatgtaggtg caacctatta
    34501 cttcaacaaa aatatgtcca cttttgttga ttacaaaatc aacctgattg ataagagcga
    34561 tttcacgaaa gcatctggcg ttgctaccga tgatatcgtt gctgtaggat tggtttacca
    34621 gttctaatct gattactcaa agatatgttg tgggaggctt tgcctcccca acatataagt
    34681 ggcgctctca agccactttc cttagaagca ctatcttgct tctgactata taaattaccc
    34741 tttattttct gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt
    34801 atcaattaga tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca
    34861 tccctttaat tatcccctca acctcttgta aatttcccac tcttgagctc cacatagccc
    34921 aaacgccgga tgcaaaaaaa atcacacgtg catctctgaa attgtgatgg aggaagtgag
    34981 tatcaattat atgtgcttat aaacgttccc gattggcaca gaagtcaccg gattgttcag
    35041 gctccaatag gttgagtatg tcgtatttat tttccaaaat aaaaatcgat gctgttgctc
    35101 cttaataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg ttatattaaa
    35161 acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat
    35221 ctcgctgatg ggttttactg tcgtttgtat agcaggacaa cgcattattt aacctcacgt
    35281 cgtccaatat actttgaaaa cttattccct cgcttgccag acggcgatgc aatgtggaaa
    35341 cagaaatgta gagatatctc gctaccttgt ttgctgtcca ttttgttcta ggttcggata
    35401 gcattaggtt ataacaacga cttattaatg attgtttact atctgataaa agtaaacaat
    35461 taacatgatg tgctcctaat gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg
    35521 ttgagaagcc tcgggaaata gatgttttta gttgctccca acaatatatt aactcggggt
    35581 tctgaggtaa gcagaaacct gtcgtgttac gaatttgatc agttatcgca taaaattttt
    35641 gaaacttttc aattagatca atgggataat aaagcatttc tgcaagataa agacctgctt
    35701 ctggataatt cacaatataa aattcatatc cacagggaaa taatattatc tgattattat
    35761 caacagttag actatgcgtc tcccaattga taactttttt tccctgacgg atacgacata
    35821 aagctggcat aagaggctta accctgtgaa tctcatgatg tttatgcatc cgtatttctt
    35881 caatctttag gttagactta tctcttgcca gcatactctt accctatttt atctcataaa
    35941 ctggtgttat ctcagcggtt gcaattttat tggcattaag catataacca actaacgctc
    36001 cgctggaatt agaatccaca ggaatcttat cagtttttag agcccatact ttaaactggt
    36061 aatgatgggg tttatctcct ttgggaggac atgctccacc aaatcctgca taaccaaaat
    36121 catttcggcc ttgaacagca ccagtcggaa gttttgtacc atcacgtctt cctgcatcag
    36181 tgggcaaata tgttacagtt gctggaatat tagcaacagt ccagtgccac catccactgc
    36241 ctgtaggagc atctggatca tacacagtta cggcaaagct tttggtgcct tcaggagcac
    36301 cagaccaggt taatgagggc gatgtattac caccttcaca cccaaatcca gaaaatacat
    36361 gagacgttgt aagttgctct cctgttttta tttcattact agtgacctga aatgctgcgg
    36421 cctgcgcaga aaatgttatg aatgccaata cacttgaaac gataagtttt ttcataaaaa
    36481 cctcattgtt gtgacctatc gttattttat ttgatgtgcc ttgatctcat tatgaataaa
    36541 ggcgcaatga tcgcgcaaat gcaatcataa ttataaaggt gccccgaaca ttagccataa
    36601 tatgcacaaa tcagattcct gttatctcaa ggagaattat tttttaatgc ggtgcacttc
    36661 gtgacaacaa gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatagctg
    36721 gcaggtccag tcgttgacca aaaaatcccc aggaaagcaa actaatcaga acgataccga
    36781 ctcctgacca gatagcataa gcaatccctg taggtatata agccagcgtc tgagctaata
    36841 accagaatga tgcacaatag caaataattg tgccaacaga gggccataac cgtgtaaacc
    36901 cctctgaaaa cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat
    36961 aaatataagg gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt
    37021 ttgaacacaa ctcaccgttt tcatcctggt gtatgtgtat ccaaggggct ctggcgtggc
    37081 aagagataaa ggcaggaaca ataatgatca gaaggaaccc caggctaacg aatgagttca
    37141 ctcttcctgg gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc
    37201 agaaaacaac caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc
    37261 catgccccac gctgtgggat cggatataat taaacaagca tagtcaacag gttacacaac
    37321 ctacacctgc atggacatca cttcctgata cgccgccacc agcttattac gcacctgaat
    37381 ccccatttgc atagaaactg aggctttttg catatcggtc atcacatcgt ttagcgccac
    37441 gccgggttca ccgagggtga atttttctgc ctgcgtgcgg gcgactgttt gtgtatcgct
    37501 tatgcgatcg agcgcggcgt gcagctgccc ggcaaaacta atggtcggtt gcggcagtga
    37561 ttcctgcgca cacgcactca tcgccgtagc ctgtaactgg ctgataaccc cttcaatccc
    37621 ctgtatcgct gacattttcg tctcccggat aatttctggt aacaaagcct accagtaagt
    37681 caataagaca aaggcgctaa atagcaacaa aaaaactggt ttattggcgg atagaaaaaa
    37741 cgaaagcaca aataatggga gcgtcaattt ttcgagtttg ctgacccgag agtgagtctt
    37801 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
    37861 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
    37921 ttgattgtta ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
    37981 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
    38041 agccaactga cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgag
    38101 gttccggcag ataaagttca cgaactgcgc ctgcgcctgg cgcaacaagg tttgcccaaa
    38161 ggcggcgcgg tcggtttcga actgctcgat caggaaaagt ttggtatcag ccagttcagc
    38221 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
    38281 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
    38341 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
    38401 gaagggcaaa ttagcgctat tgtacatctg gtttccagcg cggttgctgg tctgccgcct
    38461 ggaaacgtca cgctggtgga tcagggcggg catctgttaa cccagtccaa taccagcggg
    38521 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcag
    38581 cgtattgaag cgatcctgtc acctattgtt ggtaacggta atattcacgc ctaggtcacg
    38641 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
    38701 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
    38761 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
    38821 atcagcacgc ctccgacaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
    38881 agtgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
    38941 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
    39001 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
    39061 gaagctctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt
    39121 gttaactcgc cgttcaatag cagtgacgaa agcggcggag cactgccgtt ttggcaacag
    39181 caagtgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc tggtactgct
    39241 ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc gcgccgaggc
    39301 ggtgaagact gtgcagcaac aggcgcaggc ccacgaggaa gtggaagatg cggtggaagt
    39361 ccgtctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac tgggggcaga
    39421 agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt
    39481 cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat aaaagcgtca
    39541 tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac ctctcccagc
    39601 gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc
    39661 taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgctgca ctgaatatca
    39721 acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa cgtgccgcca
    39781 gcctgctgga agatattctc gaaacccgcg ataccgccag cggtattgaa acgctcaact
    39841 ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa attatcgcca
    39901 ccattctggt gcatctgaag cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac
    39961 gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc
    40021 tggccgaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc aagcgcagca
    40081 aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg cagcaggaag
    40141 aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa atcatcgacg
    40201 agatgttcct gttcgagaat ctggtggatg tcgatgatcg cagcattcag cgtctgttgc
    40261 aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag ccactgcgcg
    40321 agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca
    40381 accgtggtcc ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt ctgctgattg
    40441 tgcgccgtct tgccgaaact ggcgagatgg tgattggcag cggcgaggat acctatgtct
    40501 gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccacc ggcagagttt
    40561 gtgccaatgg tcgagtcgga agaaaccatt attgaagagg ccgaacccag ccttgagcag
    40621 caactggcgc aactgcaaat gcaggcccac gagcagggtt atcaggcggg gattgccgaa
    40681 ggtcgccagc aaggtcacga acagggctat caggaaggac tggcccaggg gctggaacaa
    40741 ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg cccggatgca gcaactggtc
    40801 agcgaatttc aaactaccct tgatgcgctt gatagtgtga tagcttcgcg cctgatgcag
    40861 atggcgctgg aggcagcacg tcaggtgatc ggtcagacac caacggtgga taactcggca
    40921 ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg gaaaccacag
    40981 ctgcgcgtac acccggatga tctgcaacgt gtggatgata tgctcggcgc aactttaagt
    41041 ttgcatggct ggcgcttgcg gggcgatccc accctccacc ctggcggatg taaagtctcc
    41101 gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact ctgccgtctg
    41161 gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc acgctggata
    41221 actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga ttaacccgcg
    41281 ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca acctgtgtca
    41341 ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg
    41401 gtcaacggct gtttttaatg ccgctggagg aagtggaagg tgtcctgccc ggcgcgcgtg
    41461 tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg caagcagttg ccgctcggtc
    41521 cggcgttatt aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc ctgcccttcc
    41581 ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccgttg caacgtacac
    41641 cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt accgtcggac
    41701 gcgggcagcg tatggggctg tttgccgggt cgggcgttgg taaaagtgtg ctgctgggga
    41761 tgatggcacg ctacacccgc gccgatgtca ttgtcgtggg tttgattggt gaacgtgggc
    41821 gcgaagtaaa agattttatt gagaatatcc tcggtgccga aggacgtgca cgctcagtgg
    41881 tgattgccgc tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc gcctatgcca
    41941 cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt atggactccc
    42001 tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa ccacctgcca
    42061 ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc actggtcgag cgtgccggta
    42121 atggcattag cggcggcggc tcgattaccg cgttttatac cgtgctcacc gaaggcgatg
    42181 accagcagga ccccattgct gactccgcgc gggcaatcct cgacggtcac attgtgctgt
    42241 ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg tcgatcagtc
    42301 gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc
    42361 tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca
    42421 gcgatccgat gctcgataaa gccatcgccc tgtggccgca gctggagggc tatttgcaac
    42481 aaggcatttt tgaacgcgcg gactgggaag cgtctctcca ggggctggag cgtattttcc
    42541 cgacagtgtc ataacccagg agataacggc agatggcgga agaacagctc aaaatgttga
    42601 tcgattatca gaatgaatat cgcaataacc tcaatagcga tatgagtgcc gggatgacca
    42661 gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc
    42721 atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag
    42781 aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac
    42841 tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca
    42901 tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg acaccaccac
    42961 attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt tgagcgaagc
    43021 attagcaggc gagacaacaa ctgacaaagc ggccccccag ttgctggtag caacagataa
    43081 gcccacgaca aaaggcgagc cgctggtcag cgatattctt gccgatgcgc aacaagcgga
    43141 tttactgatc cctgtggatg aaacacttcc tgtcatcaac gacgaacaat ccacatcaac
    43201 accgttaacc accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa atacgacaaa
    43261 agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg ccctttttgc
    43321 gatgttgccg ggttttgaca atacgcccaa agtgactgat gcaccgtcaa ccgtgttacc
    43381 ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacta cagcacagcc
    43441 tgatgacgcc cccggcacac cagctcagcc attaacaccg ctggtagcag aagcccagag
    43501 taaagcggaa gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc cgctaatcac
    43561 tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg caccgctggg
    43621 ttctcacgaa tggcaacaat cattgagcca gcatatttcg ctgttcaccc gccaggggca
    43681 acaaagtgca gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa tttccctcaa
    43741 agtggatgat aaccaggcgc agatccagat gatttcaccg catcagcacg tacgcgccgc
    43801 cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg
    43861 gcaaagcaac attagtggcg aaagctttag tggtcagcag caggccgctt cccagcaaca
    43921 gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc
    43981 ggttcccgtc tctttacaag ggcgtgtaac aggtaacagc ggcgttgata ttttcgccta
    44041 acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag
    44101 gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg
    44161 ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg
    44221 gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa
    44281 gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt tacggtcaat
    44341 ttgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgactgaa agatgaagca
    44401 acccgctcgc ggctgagtga gtatttgccg gaagtccgta gtcgcttgct gttactgttt
    44461 tcacgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag
    44521 attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac
    44581 gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc
    44641 tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt
    44701 tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga
    44761 acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct
    44821 gttcaacctg ctgcgccgta gcccggacat taccgtcggg gccatccgca ttcagccgta
    44881 ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc acctgaaacc
    44941 gctacgcggc acagggctgg tagtgttctc gccgagtctg gtgtttatcg ccgtggataa
    45001 cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac
    45061 tgaacagcgc gtcatcaacc gtatgttgaa gctggcgctg gaaggctata gcgacgcctg
    45121 gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tggaatttac
    45181 caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg
    45241 caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc cgctacggga
    45301 attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa
    45361 cctggtgcgc caggtgcagc attcacagct ggaactggtg gccaactttg ccgatatctc
    45421 gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga tagaaaaacc
    45481 cgatcgcatc atcgcccatg ttgacggcgt cccggttctg accagtcagt acggtactct
    45541 caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa
    45601 cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg
    45661 gacgatctgt gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa aagcgctgcc
    45721 gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac
    45781 ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc
    45841 aaagagctgt tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct ggcgggcgaa
    45901 ccgctggata ttctgatcaa cggttattta atcgcccagg gcgaagtggt ggttgttgcc
    45961 gataaatatg gcgtgcggat caccgatatc attaccccgt ccgagcggat gcgccgcctg
    46021 agccgttagt gatgaataac catgctactg tgcaatcttc cgcgccggtt tctgctgagc
    46081 cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct
    46141 ggctggtaaa acggctggga tttgccccta aacgcactgg cgttaacggt ctgaaaatta
    46201 gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg gaagatgcac
    46261 ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag
    46321 caccaacgga agagataccg cagaccgatt ttcagtcggt catgaaaaat ttgcttaagc
    46381 gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc tctggctgat
    46441 tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctac ctggcggtgg
    46501 acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga cgttcattcc
    46561 agcgatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg gtttattgcg
    46621 taacgcgctg ggaacgccct ccgcgccacc taaccaggta ttgctggggc tggcactgtt
    46681 tttgaccttt tttattatgt cgccagtgat caacaaaatt tatgtcgatg cgtaccagcc
    46741 attcagcgaa gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg
    46801 tgagtttatg ctgcgtcaga cccgcgaggc agatttaggg ttgtttgcca gactggcgaa
    46861 taccggcccg ttgcaggggc ctgaagctgt gccgatgcgc attttgctcc cggcctacgt
    46921 gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc cttttttgat
    46981 tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttccccccgc
    47041 caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct
    47101 ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt
    47161 catgatgatg ggaactgaag cgatgaaagt cgcgctggca ctggcagccc cgctgttgct
    47221 ggtagcgctg gtcacgggcc ttatcatcag tattttgcag gcagccacgc agattaacga
    47281 aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatca ttattgccgg
    47341 accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta
    47401 tatcatcggg tagcccgtac aatgatgcag gagacaagcg atcaatggct ttcctggtta
    47461 agcctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc gccaattctg
    47521 agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgcc
    47581 attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg
    47641 gccgtacagc agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc
    47701 gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt tgcgacattt
    47761 gtcgatcctg gcagccatct caatatgccc gttttagcgc gcatcatgga tatgctggca
    47821 ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc
    47881 tttcacaccc tgccgattgg tagcgaaccg ttgaacagca atgcgtttct ggcacccacc
    47941 aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg
    48001 ttgctgaccc tgaatctggc attaggttta ctgaatcgta tggccccgca attatccatt
    48061 tttgttattg gatttccatt aactctgact gtcggcatct ctttaatggc ggcattaatg
    48121 ccgttaattg cgcctttttg cgaacattta ttcagtgaaa tttttaattt gctggctgat
    48181 attattagtg aattaccatt aatataattc cgtaacgttt atcatgctac cctaaggatt
    48241 atccgaaaaa taatatctgc gaacatcttc caggatactc ctgtagcgaa atatttgttt
    48301 taagctcact cacatatcgc aacatttact ttaagacaat tccaggcaaa ttatacaaca
    48361 ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg
    48421 ttgttccgtt tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt
    48481 gagggtatgc catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa
    48541 ccgggtatct gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg
    48601 atgaccttgc catagcttgt gattcacagc gtccttcagt ggtgtttatt aatgaggact
    48661 gtttcatcca cgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca
    48721 atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg
    48781 tcagaaaaaa tttattgatc agttctaaat cgattaaacc ggaatctctc gacgatatcc
    48841 ttggcaatat tctgaaaaaa gagacaacga taacctcgtt tttaaatatg ccgacgttat
    48901 cattgagccg aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc
    48961 aaatctccga ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta
    49021 aacgtaagat caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata
    49081 atgtgactaa tggtattttt gtcaacatgc gctaacacat tctgactggt gatttcccac
    49141 cagtcaggct gaataagatt actctgcttt ctcaacaaag ataccgtcct gatgccctgc
    49201 ttcattaaag aaccagatgc cgagcgggta gtcttccagc gcaaccaggt acattgtgcc
    49261 ttcactaaac tcctcaactg ccagtaccac tccaggacga cgcggaccgc catccgtttt
    49321 gactgttacc cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga
    49381 acaatttcgt ttttctggca gcgccaagcg cgcgcgagtg ctgattttct cgacggtcta
    49441 tacttaagag atgctaggcg gacttaacga ctggcggcaa caacagagta acggttgcga
    49501 ggaaagatga tgaaaaccgc aaaagtgtac agcgataccg caaaacgtga ggtcagcgtc
    49561 gatgtcgatg ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc
    49621 cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg
    49681 gcggatgcct tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatt
    49741 cacaataaaa aaatcccgac cctgagggtg tcgggatgaa acttgcttaa gcaagaagca
    49801 cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatct gcaatttttt
    49861 tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg
    49921 acgcgcgtca ccccttggtg gctctggtgt caacgggatc gctcccgaaa aaatcatctt
    49981 tgattatttg tgctgttttg agaatcatcc tggtaagggg agtactgcgc gaccatgttt
    50041 tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct ggacagtcat
    50101 agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc caatattccc
    50161 gtcattctct gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa aatgttgggg
    50221 ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg
    50281 caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga aatctgccag
    50341 gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac
    50401 gatgcaacca ttgccgaatg gacgggatta agccgtagct aggcggcgct gacgcagctg
    50461 catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc
    50521 gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg
    50581 gatgcctctg ccggaaaaga tcaggctgcc aattggatta tcgcgaccta tcaacaattg
    50641 tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg
    50701 gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt acatctgcat
    50761 gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa
    50821 gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc
    50881 ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca
    50941 taatcaccgg tttcctcgct gctacttacc cccagcgagg cactgatgcg tatcgttgta
    51001 ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc
    51061 gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc
    51121 ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca
    51181 cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc
    51241 tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt
    51301 tcgaacagtg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc
    51361 aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata ccaggagagg
    51421 agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa
    51481 tcgccgcgca cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc gaaatggtcc
    51541 agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc
    51601 attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca
    51661 ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg
    51721 tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc
    51781 agcacgccat accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca
    51841 gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct
    51901 cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca
    51961 ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc
    52021 gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt
    52081 gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggaaatt cccctcgctg
    52141 actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc
    52201 caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa
    52261 tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg
    52321 ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctagctg
    52381 gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc
    52441 aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt
    52501 ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg
    52561 cttttttatg attctggcat aacccggcaa tagcgtaccg caaaacagcg ataattttat
    52621 aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca ccaggccgga
    52681 tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct
    52741 cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc
    52801 aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt
    52861 taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac
    52921 ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag tattaatgtg
    52981 cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg ccgatgataa
    53041 atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc
    53101 cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc
    53161 cgacgaggaa catcgccagc gtgccgacaa tcgataatgc tttcatcagc caaggcgcga
    53221 taatcaataa ccctttacct aatgcctgca tcagcgcgct agatttttcc gccagccaat
    53281 accctaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta
    53341 ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcctct gccacaatcc
    53401 ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc gcccctttaa
    53461 ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctggc
    53521 tctgcgctgg atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc
    53581 cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa
    53641 acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg
    53701 ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat
    53761 tgagcgataa atcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca
    53821 cagagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg
    53881 ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca
    53941 gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct
    54001 cgccagcaga atgattttca cgtttactat gaaccacctt tttatttccg ccgtgaggga
    54061 ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt
    54121 ggggctcaac ctattttgtc attcggattg gcgtgggaag ctggcctccg ttaatgatgg
    54181 cgggcgttcg attccaggca gccggtattt tattgctggc atttttgcta ctgcgcggac
    54241 acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg
    54301 ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg
    54361 ccgtagtgat tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg tttggcatta
    54421 aaacgcgcaa actggaatgg gtgggtattg ccattgggct tgccggaatc atcatgctca
    54481 atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga
    54541 ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta gggatgatgg
    54601 cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg
    54661 gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc tatctggcgc
    54721 tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat gtcagtccgg
    54781 ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac
    54841 tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gttttcgcgg
    54901 tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta
    54961 ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
    55021 tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc
    55081 ctacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg caagcgactg
    55141 atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa ctcagttcga
    55201 gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat
    55261 atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga
    55321 acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta
    55381 tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc
    55441 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
    55501 tgccaccgcg gcaaagaccg gcacgaccac cgagttgccg aactggcgat aggcctgagt
    55561 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
    55621 gcattcacga ggcgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc
    55681 tttctcaccc gtggccatat cccaaccgcg atcgattaaa atttccgcgc catctttgta
    55741 ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata
    55801 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
    55861 caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac
    55921 tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag
    55981 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc
    56041 atcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt catagcccag
    56101 ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt
    56161 tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc
    56221 aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
    56281 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg
    56341 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg
    56401 actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg
    56461 gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc
    56521 accaatcgat tcaaaaccac gacggatgcc gccaattccg gcaaatagat cgataaagcg
    56581 aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa
    56641 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
    56701 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcattt taacatcata
    56761 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
    56821 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
    56881 taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt
    56941 gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca tcaaacagcg
    57001 ccaagctcaa tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc cccagggctt
    57061 ccagccgatc cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc
    57121 tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat tttctccgcc ggaaattgca
    57181 cgaactcttc acggagcagg cgacgcgtct cttctgccgc caggattgaa gaacgctgcc
    57241 gttgcggatg atttttcgcc aggctcacga tatcgtgaaa ataacaggcg gttaaaatca
    57301 ccagcatatc gacatcgtca tctgccgcca gtttctgtgc cgtagcccag acacggcgaa
    57361 aatgacacag gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac cagttctcga
    57421 actgtgcctg ccagtgttgt aactccatac gcgctccgtt gccaaagagt cgcaacctta
    57481 gcagttttta ttcatagcgt ctgcaacgtg cgggacgagg tcgggtgtac catgcaatgc
    57541 cgcctaacaa ggcaccaacc agggccataa acagaaatcc gaccaatgct cctaaccact
    57601 tccccgcggt agatcctaaa tcactttcaa atcccgacac gggggcatcg gggatccggc
    57661 tcatcaccca gatgtatcgc gccattgccc agacgaaata gccacaccat gtatagaaag
    57721 cccacaatga atcgccacgg ataatctaga cacttccgag ccgttgataa tactggtttt
    57781 catattctgt cggtgacatc tgttcgctag aaccatgccg acgcttactg ttataaaaca
    57841 tttcgatgta atcaaaaata tcactgcggg cttcttcccg cgttccgtag atctttttct
    57901 ttatccgttc acgtttcaac aactggaaaa aactttctgc aaccgcatta tcatggcagt
    57961 taccgcgacg gctcatgcta ccctccaggc cgtgtgattt caggaacgac tgccactcat
    58021 ggcttgtgta ctgactgccc tgatccgaat gaaccagcac ctgtttttcg ggattacgcc
//
LOCUS       DAECNO010000012        55466 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.012, whole genome shotgun sequence.
ACCESSION   DAECNO010000012 DAECNO010000000
VERSION     DAECNO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.012"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="KKO08_000768"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="KKO08_000768"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBD6317130.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KKO08_000769"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="KKO08_000769"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBD6317131.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KKO08_000770"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="KKO08_000770"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBD6317132.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="KKO08_000771"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="KKO08_000771"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBD6317133.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="KKO08_000772"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="KKO08_000772"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBD6317134.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="KKO08_000773"
     CDS             7826..9559
                     /locus_tag="KKO08_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBD6317135.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="KKO08_000774"
     CDS             9708..10559
                     /locus_tag="KKO08_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6317136.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="KKO08_000775"
     CDS             complement(10546..10887)
                     /locus_tag="KKO08_000775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD6317137.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="KKO08_000776"
     CDS             complement(10889..11767)
                     /locus_tag="KKO08_000776"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBD6317138.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="KKO08_000777"
     CDS             complement(11733..14030)
                     /locus_tag="KKO08_000777"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD6317139.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="KKO08_000778"
     CDS             complement(14081..14401)
                     /locus_tag="KKO08_000778"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBD6317140.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="KKO08_000779"
     CDS             complement(14416..15495)
                     /locus_tag="KKO08_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBD6317141.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="KKO08_000780"
     CDS             15804..16907
                     /locus_tag="KKO08_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBD6317142.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="KKO08_000781"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="KKO08_000781"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD6317143.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="KKO08_000782"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="KKO08_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="KKO08_000783"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="KKO08_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="KKO08_000784"
     CDS             20366..20983
                     /locus_tag="KKO08_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBD6317144.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KKO08_000785"
     CDS             complement(21010..21915)
                     /gene="yijE"
                     /locus_tag="KKO08_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBD6317145.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KKO08_000786"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="KKO08_000786"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBD6317146.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KKO08_000787"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="KKO08_000787"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBD6317147.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KKO08_000788"
     CDS             complement(25757..28189)
                     /gene="metL"
                     /locus_tag="KKO08_000788"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBD6317148.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KKO08_000789"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="KKO08_000789"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBD6317149.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="KKO08_000790"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="KKO08_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBD6317150.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="KKO08_000791"
     CDS             30130..30738
                     /locus_tag="KKO08_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBD6317151.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KKO08_000792"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="KKO08_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBD6317152.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="KKO08_000793"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="KKO08_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBD6317153.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="KKO08_000794"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="KKO08_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBD6317154.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="KKO08_000795"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="KKO08_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBD6317155.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="KKO08_000796"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="KKO08_000796"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBD6317156.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="KKO08_000797"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="KKO08_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBD6317157.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="KKO08_000798"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="KKO08_000798"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBD6317158.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="KKO08_000799"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="KKO08_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBD6317159.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KKO08_000800"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="KKO08_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBD6317160.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="KKO08_000801"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="KKO08_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="KKO08_000802"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="KKO08_000802"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBD6317161.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="KKO08_000803"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="KKO08_000803"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD6317162.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="KKO08_000804"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="KKO08_000804"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBD6317163.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KKO08_000805"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="KKO08_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBD6317164.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="KKO08_000806"
     CDS             complement(44853..45152)
                     /locus_tag="KKO08_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBD6317165.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="KKO08_000807"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="KKO08_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="KKO08_000808"
     CDS             45905..46504
                     /locus_tag="KKO08_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBD6317166.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="KKO08_000809"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="KKO08_000809"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBD6317167.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KKO08_000810"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="KKO08_000810"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBD6317168.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KKO08_000811"
     CDS             complement(48296..49285)
                     /gene="sbp"
                     /locus_tag="KKO08_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBD6317169.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KKO08_000812"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="KKO08_000812"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBD6317170.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KKO08_000813"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="KKO08_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBD6317171.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KKO08_000814"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="KKO08_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBD6317172.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="KKO08_000815"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="KKO08_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBD6317173.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="KKO08_000816"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="KKO08_000816"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBD6317174.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KKO08_000817"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="KKO08_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBD6317175.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13515 a        15032 c        14167 g        12752 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttagactggc
    55441 cccctgaatc tccagacaac cagtat
//
LOCUS       DAECNO010000013        53213 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.013, whole genome shotgun sequence.
ACCESSION   DAECNO010000013 DAECNO010000000
VERSION     DAECNO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.013"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(11..322)
                     /locus_tag="KKO08_000818"
     CDS             complement(11..322)
                     /locus_tag="KKO08_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBD6317176.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="KKO08_000819"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KKO08_000819"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="KKO08_000820"
     CDS             701..1054
                     /locus_tag="KKO08_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBD6317177.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KKO08_000821"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="KKO08_000821"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBD6317178.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="KKO08_000822"
     CDS             complement(2810..3280)
                     /locus_tag="KKO08_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBD6317179.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KKO08_000823"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="KKO08_000823"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBD6317180.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KKO08_000824"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="KKO08_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBD6317181.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KKO08_000825"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="KKO08_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBD6317182.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="KKO08_000826"
     CDS             complement(5283..8432)
                     /locus_tag="KKO08_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBD6317183.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KKO08_000827"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="KKO08_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBD6317184.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="KKO08_000828"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="KKO08_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBD6317185.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="KKO08_000829"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="KKO08_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBD6317186.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KKO08_000830"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="KKO08_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBD6317187.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KKO08_000831"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="KKO08_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBD6317188.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="KKO08_000832"
     CDS             14911..15288
                     /locus_tag="KKO08_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBD6317189.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="KKO08_000833"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="KKO08_000833"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBD6317190.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="KKO08_000834"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="KKO08_000834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBD6317191.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="KKO08_000834"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="KKO08_000835"
     CDS             18099..18428
                     /locus_tag="KKO08_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBD6317192.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="KKO08_000836"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="KKO08_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBD6317193.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="KKO08_000837"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="KKO08_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBD6317194.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="KKO08_000838"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="KKO08_000838"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBD6317195.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="KKO08_000839"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="KKO08_000839"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBD6317196.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KKO08_000840"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="KKO08_000840"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBD6317197.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="KKO08_000841"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="KKO08_000841"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBD6317198.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="KKO08_000842"
     CDS             complement(25481..27157)
                     /locus_tag="KKO08_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBD6317199.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KKO08_000843"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="KKO08_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBD6317200.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="KKO08_000844"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="KKO08_000844"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBD6317201.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KKO08_000845"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="KKO08_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBD6317202.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="KKO08_000846"
     CDS             complement(31205..31999)
                     /locus_tag="KKO08_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBD6317203.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="KKO08_000847"
     CDS             32137..32478
                     /locus_tag="KKO08_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD6317204.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KKO08_000848"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="KKO08_000848"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBD6317205.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAELQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="KKO08_000849"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="KKO08_000849"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBD6317206.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="KKO08_000850"
     CDS             36394..37686
                     /locus_tag="KKO08_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6317207.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="KKO08_000851"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="KKO08_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBD6317208.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="KKO08_000852"
     CDS             complement(38219..38677)
                     /locus_tag="KKO08_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBD6317209.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="KKO08_000853"
     CDS             complement(38674..39591)
                     /locus_tag="KKO08_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBD6317210.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="KKO08_000854"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="KKO08_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBD6317211.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="KKO08_000855"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="KKO08_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBD6317212.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KKO08_000856"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="KKO08_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBD6317213.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KKO08_000857"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="KKO08_000857"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD6317214.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KKO08_000858"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="KKO08_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="KKO08_000859"
     CDS             43550..44236
                     /locus_tag="KKO08_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6317215.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="KKO08_000860"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="KKO08_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="KKO08_000861"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="KKO08_000861"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBD6317216.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="KKO08_000862"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="KKO08_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBD6317217.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="KKO08_000863"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="KKO08_000863"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBD6317218.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="KKO08_000864"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="KKO08_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBD6317219.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="KKO08_000865"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="KKO08_000865"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBD6317220.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53213
                     /locus_tag="KKO08_000866"
     CDS             52938..>53213
                     /locus_tag="KKO08_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBD6317221.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIR"
BASE COUNT        12829 a        13691 c        14021 g        12672 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagctccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt cgt
//
LOCUS       DAECNO010000014        52075 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.014, whole genome shotgun sequence.
ACCESSION   DAECNO010000014 DAECNO010000000
VERSION     DAECNO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.014"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KKO08_000867"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="KKO08_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBD6317222.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="KKO08_000868"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="KKO08_000868"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD6317223.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="KKO08_000869"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="KKO08_000869"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD6317224.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTAQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="KKO08_000870"
     CDS             complement(5906..6949)
                     /locus_tag="KKO08_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBD6317225.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KKO08_000871"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="KKO08_000871"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBD6317226.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KKO08_000872"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="KKO08_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBD6317227.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="KKO08_000873"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="KKO08_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="KKO08_000874"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="KKO08_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBD6317228.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KKO08_000875"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="KKO08_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBD6317229.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="KKO08_000876"
     CDS             15474..15830
                     /locus_tag="KKO08_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBD6317230.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="KKO08_000877"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="KKO08_000877"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBD6317231.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="KKO08_000878"
     CDS             16989..17189
                     /locus_tag="KKO08_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBD6317232.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KKO08_000879"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="KKO08_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD6317233.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KKO08_000880"
     CDS             complement(18071..20086)
                     /gene="tmcA"
                     /locus_tag="KKO08_000880"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBD6317234.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="KKO08_000881"
     CDS             complement(20101..20964)
                     /locus_tag="KKO08_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBD6317235.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KKO08_000882"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="KKO08_000882"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBD6317236.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGVRIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KKO08_000883"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="KKO08_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBD6317237.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KKO08_000884"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="KKO08_000884"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBD6317238.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="KKO08_000885"
     CDS             24133..24705
                     /locus_tag="KKO08_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBD6317239.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="KKO08_000886"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="KKO08_000886"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBD6317240.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="KKO08_000887"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="KKO08_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBD6317241.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPTCVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="KKO08_000888"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="KKO08_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="KKO08_000889"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="KKO08_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBD6317242.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /gene="hyfD"
                     /locus_tag="KKO08_000890"
     CDS             29037..30476
                     /gene="hyfD"
                     /locus_tag="KKO08_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBD6317243.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="KKO08_000891"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="KKO08_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBD6317244.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="KKO08_000892"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="KKO08_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBD6317245.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="KKO08_000893"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="KKO08_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="KKO08_000894"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="KKO08_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBD6317246.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="KKO08_000895"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="KKO08_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBD6317247.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="KKO08_000896"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="KKO08_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBD6317248.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /gene="hyfR"
                     /locus_tag="KKO08_000897"
     CDS             36179..38191
                     /gene="hyfR"
                     /locus_tag="KKO08_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBD6317249.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="KKO08_000898"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="KKO08_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="KKO08_000899"
     CDS             complement(39099..40160)
                     /locus_tag="KKO08_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBD6317250.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="KKO08_000900"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="KKO08_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBD6317251.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="KKO08_000901"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="KKO08_000901"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBD6317252.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KKO08_000902"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="KKO08_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBD6317253.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KKO08_000903"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="KKO08_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBD6317254.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KKO08_000904"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="KKO08_000904"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBD6317255.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="KKO08_000905"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="KKO08_000905"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBD6317256.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="KKO08_000906"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="KKO08_000906"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBD6317257.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="KKO08_000907"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="KKO08_000907"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBD6317258.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="KKO08_000908"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="KKO08_000908"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBD6317259.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="KKO08_000909"
     CDS             complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="KKO08_000909"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBD6317260.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12157 a        13738 c        13520 g        12660 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacggctc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcacgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccgacctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgattctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgctt
//
LOCUS       DAECNO010000015        49891 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.015, whole genome shotgun sequence.
ACCESSION   DAECNO010000015 DAECNO010000000
VERSION     DAECNO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.015"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..342
                     /locus_tag="KKO08_000910"
     CDS             <1..342
                     /locus_tag="KKO08_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6317261.1"
                     /translation="DQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRK
                     GNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPI
                     EYRNQTYMPRV"
     gene            444..596
                     /gene="hokA"
                     /locus_tag="KKO08_000911"
     CDS             444..596
                     /gene="hokA"
                     /locus_tag="KKO08_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBD6317262.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(783..995)
                     /gene="cspA"
                     /locus_tag="KKO08_000912"
     CDS             complement(783..995)
                     /gene="cspA"
                     /locus_tag="KKO08_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBD6317263.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1276..1566)
                     /locus_tag="KKO08_000913"
                     /pseudo
     CDS             complement(1276..1566)
                     /locus_tag="KKO08_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2000..2710
                     /locus_tag="KKO08_000914"
     CDS             2000..2710
                     /locus_tag="KKO08_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBD6317264.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2760..3734)
                     /gene="ghrB"
                     /locus_tag="KKO08_000915"
     CDS             complement(2760..3734)
                     /gene="ghrB"
                     /locus_tag="KKO08_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBD6317265.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3838..4488)
                     /locus_tag="KKO08_000916"
     CDS             complement(3838..4488)
                     /locus_tag="KKO08_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBD6317266.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4651..6984
                     /gene="bisC"
                     /locus_tag="KKO08_000917"
     CDS             4651..6984
                     /gene="bisC"
                     /locus_tag="KKO08_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBD6317267.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6953..7393)
                     /locus_tag="KKO08_000918"
     CDS             complement(6953..7393)
                     /locus_tag="KKO08_000918"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBD6317268.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7390..7953)
                     /gene="tag"
                     /locus_tag="KKO08_000919"
     CDS             complement(7390..7953)
                     /gene="tag"
                     /locus_tag="KKO08_000919"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBD6317269.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8111..8809
                     /locus_tag="KKO08_000920"
     CDS             8111..8809
                     /locus_tag="KKO08_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD6317270.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9038..10246
                     /locus_tag="KKO08_000921"
     CDS             9038..10246
                     /locus_tag="KKO08_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6317271.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10570..12261
                     /gene="eptB"
                     /locus_tag="KKO08_000922"
     CDS             10570..12261
                     /gene="eptB"
                     /locus_tag="KKO08_000922"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBD6317272.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12353..12429
                     /locus_tag="KKO08_000923"
     tRNA            12353..12429
                     /locus_tag="KKO08_000923"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12387..12389,aa:Pro,seq:cgg)
     gene            13173..14780
                     /gene="dppA"
                     /locus_tag="KKO08_000924"
     CDS             13173..14780
                     /gene="dppA"
                     /locus_tag="KKO08_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBD6317273.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15088..16107
                     /gene="dppB"
                     /locus_tag="KKO08_000925"
     CDS             15088..16107
                     /gene="dppB"
                     /locus_tag="KKO08_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBD6317274.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16117..17019
                     /gene="dppC"
                     /locus_tag="KKO08_000926"
     CDS             16117..17019
                     /gene="dppC"
                     /locus_tag="KKO08_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBD6317275.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17030..18013
                     /gene="dppD"
                     /locus_tag="KKO08_000927"
     CDS             17030..18013
                     /gene="dppD"
                     /locus_tag="KKO08_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBD6317276.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18010..19014
                     /gene="dppF"
                     /locus_tag="KKO08_000928"
     CDS             18010..19014
                     /gene="dppF"
                     /locus_tag="KKO08_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBD6317277.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19044..20315)
                     /locus_tag="KKO08_000929"
     CDS             complement(19044..20315)
                     /locus_tag="KKO08_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD6317278.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20791..20898
                     /locus_tag="KKO08_000930"
     CDS             20791..20898
                     /locus_tag="KKO08_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD6317279.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21274..21381
                     /gene="ldrD"
                     /locus_tag="KKO08_000931"
     CDS             21274..21381
                     /gene="ldrD"
                     /locus_tag="KKO08_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBD6317280.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21757..21864
                     /locus_tag="KKO08_000932"
     CDS             21757..21864
                     /locus_tag="KKO08_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD6317281.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            22240..22347
                     /locus_tag="KKO08_000933"
     CDS             22240..22347
                     /locus_tag="KKO08_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD6317282.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22434..24113)
                     /gene="bcsG"
                     /locus_tag="KKO08_000934"
     CDS             complement(22434..24113)
                     /gene="bcsG"
                     /locus_tag="KKO08_000934"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBD6317283.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(24110..24301)
                     /gene="bcsF"
                     /locus_tag="KKO08_000935"
     CDS             complement(24110..24301)
                     /gene="bcsF"
                     /locus_tag="KKO08_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBD6317284.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(24298..25869)
                     /gene="bcsE"
                     /locus_tag="KKO08_000936"
     CDS             complement(24298..25869)
                     /gene="bcsE"
                     /locus_tag="KKO08_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBD6317285.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            26142..26330
                     /locus_tag="KKO08_000937"
     CDS             26142..26330
                     /locus_tag="KKO08_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBD6317286.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            26342..27094
                     /gene="bcsQ"
                     /locus_tag="KKO08_000938"
     CDS             26342..27094
                     /gene="bcsQ"
                     /locus_tag="KKO08_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBD6317287.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            27091..27450
                     /locus_tag="KKO08_000939"
     CDS             27091..27450
                     /locus_tag="KKO08_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBD6317288.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            27447..29708
                     /gene="bcsA"
                     /locus_tag="KKO08_000940"
                     /pseudo
     CDS             27447..29708
                     /gene="bcsA"
                     /locus_tag="KKO08_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29719..32058
                     /gene="bcsB"
                     /locus_tag="KKO08_000941"
     CDS             29719..32058
                     /gene="bcsB"
                     /locus_tag="KKO08_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBD6317289.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADATIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            32065..33171
                     /gene="bcsZ"
                     /locus_tag="KKO08_000942"
     CDS             32065..33171
                     /gene="bcsZ"
                     /locus_tag="KKO08_000942"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBD6317290.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            33230..33406
                     /locus_tag="KKO08_000943"
                     /pseudo
     CDS             33230..33406
                     /locus_tag="KKO08_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            33734..35482
                     /gene="bcsC"
                     /locus_tag="KKO08_000944"
                     /pseudo
     CDS             33734..35482
                     /gene="bcsC"
                     /locus_tag="KKO08_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            35564..37552
                     /gene="hmsP"
                     /locus_tag="KKO08_000945"
     CDS             35564..37552
                     /gene="hmsP"
                     /locus_tag="KKO08_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBD6317291.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            37737..39023
                     /gene="dctA"
                     /locus_tag="KKO08_000946"
     CDS             37737..39023
                     /gene="dctA"
                     /locus_tag="KKO08_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBD6317292.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            39244..40740
                     /locus_tag="KKO08_000947"
     CDS             39244..40740
                     /locus_tag="KKO08_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD6317293.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(40836..41765)
                     /gene="kdgK"
                     /locus_tag="KKO08_000948"
     CDS             complement(40836..41765)
                     /gene="kdgK"
                     /locus_tag="KKO08_000948"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBD6317294.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            41997..42764
                     /gene="pdeH"
                     /locus_tag="KKO08_000949"
     CDS             41997..42764
                     /gene="pdeH"
                     /locus_tag="KKO08_000949"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBD6317295.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            42834..44894
                     /locus_tag="KKO08_000950"
     CDS             42834..44894
                     /locus_tag="KKO08_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD6317296.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(45076..46398)
                     /locus_tag="KKO08_000951"
     CDS             complement(45076..46398)
                     /locus_tag="KKO08_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBD6317297.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(46809..47822)
                     /gene="yhjD"
                     /locus_tag="KKO08_000952"
     CDS             complement(46809..47822)
                     /gene="yhjD"
                     /locus_tag="KKO08_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBD6317298.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(47871..48770)
                     /locus_tag="KKO08_000953"
     CDS             complement(47871..48770)
                     /locus_tag="KKO08_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6317299.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            49290..>49891
                     /locus_tag="KKO08_000954"
     CDS             49290..>49891
                     /locus_tag="KKO08_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD6317300.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11974 a        12434 c        13462 g        12021 t
ORIGIN      
        1 gatcaggcat tcaaaaagct taatcctcac gagcatcctg ttctgcactc tgaccaggga
       61 tggcagtatc gtatgagaag atatcaaaat atccttaaag aacatggtat taaacaaagc
      121 atgtccagaa aaggcaattg tctggataat gctgtggtgg agtgtttctt tggaacctta
      181 aagtcggagt gtttttatct tgatgagttc agtaatataa gcgaactgaa ggatgctgtt
      241 acggaatata ttgaatacta taacagcaga agaattagcc tgaaattaaa aggtctgact
      301 ccaattgaat atcggaatca gacctatatg cctcgtgttt aactgtccaa ctttttgggg
      361 tcagtacaaa gtgaggctac cctatgcctg aacaccataa ggttagcctc ttactcgttg
      421 gaaatcaaca cagggggctg ggaatgccgc agaaatatag attactttct ttaatagtga
      481 tttgtttcac gcttttattt ttcacctgga tgataagaga ttcactgtgt gaattgcata
      541 ttaaacagga gagttatgag ctggcggcgt ttttagccta caaattgaaa gagtaagagt
      601 cttcggcggg aaattattcc cgccttactt acggcgttgc gcattctcat tgcacccata
      661 tttattcttc acaaaaataa taatagattt tattacgcga tcgattattt atttcctgaa
      721 aaaaaataaa aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga
      781 gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag
      841 gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt
      901 acgaacacat ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg
      961 aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt
     1021 gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta
     1081 cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct
     1141 gtacgtcaaa ccgttgatgt gcattaagcc actcattggc gggtgatgca acaattattt
     1201 ttcatattta tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat
     1261 aaagtggata aaagtctatt tcatcaactg cttacttaat gccgggttgg cttgaatcaa
     1321 acgcatcaat tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac
     1381 catggcgact gatacgccca aaacccgggc gaaatcatcg atttttaatc ctgtcccttt
     1441 tcataactgc tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt
     1501 ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata
     1561 ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga agctcattaa
     1621 gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc
     1681 gattcagcct gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat
     1741 atctcttatc actcctgttt tgtaaagatt tcgcaaatct aaccttctgc gaaggcgtaa
     1801 atctctggct acaaaaaagg caacggcact atcatttttt aacaatgaaa caacaaatta
     1861 accttgtttc attttctcca tgattttacc aggaagatta cgtagaaagg gtatcctgca
     1921 agcctgtcct ggactatcct tgtcacgtca gacacgcgtg tgtcgttgtg cgcttttttt
     1981 gggtgaaagg agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt ttgcgcctct
     2041 tgcggcgtta ttaatggtag ttagcctgag tgggtgtttt gataaagaag gcgatcagcg
     2101 taaagcgttt atcgacttcc tgcaaaatac agtgatgcgt agcggtgaac gtctaccaac
     2161 cctgactgcc gatcagaaaa aacagtttgg tccttttgtc tctgattacg cgattctgta
     2221 tggttattct cagcaggtga atcaggcgat ggattccggt ctgcgcccgg tcgtagacag
     2281 cgttaacgca attcgcgtac cgcaggatta cgttacgcaa agcggtccgc tgcgtgaaat
     2341 gaatggttcg ctgggtgtgc tggcgcaaca actgcaaaat gcgaagttgc aggccgatgc
     2401 tgcgcactct gcgctaaaac agagcgatga cctgaaaccg gtatttgatc aggcattcac
     2461 caaagtggta acgacgccag ctgatgcatt gcaaccgttg attccggcgg cacaaacttt
     2521 cacgcaacaa ctggtgatgg ttggagacta cattgctcag caaggtactc aggtgagctt
     2581 tgtcgcaaat ggcatccagt tcccgacttc acagcaggca agtgaatata acaaactgat
     2641 tgcgccatta ccggcacaac atcaggcgtt taatcaggcc tggactacag cagttactgc
     2701 aacccaataa agagtaaaag cccgagcgat tatcgggctt tacttcacgc agtcgcagct
     2761 tagtccgcga cgtgaggatt cacacagttc ttctcaacct ttccttgtaa cgcatcaatc
     2821 aaattatcca cggcacaggc ggccatgcca taacgcgtct catgggtggc agatccaata
     2881 tgcggtactg cgacgacgtt ggccattgag agcaacggcg aatctacgga cagtggctct
     2941 tgttcgaaga catccagccc ggcggcgtga atttcgcctt tctgcaatgc tgcgatcagt
     3001 gcattttcgt caaccaccgg gccacgtccg gcattaatga aaatggcgga ggatttcatt
     3061 ttggcgaatt gttctgcgcc aaacagatga tgcgtctcat cagttaacgg caggatcagg
     3121 caaacgaaat ctgactcttg taacagagta tccaaatcgc agtagcgggc gttgaagcgt
     3181 tcttctgctt ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt gaagccaaag
     3241 tgcacacgtt gtgccagcgc catgccgatc cgtcccatcc cgacaatgcc cagtgtttta
     3301 tggtgaacgt cagtgccgta ccagtccggg cctatgctcg cggtccattc gcctgctttt
     3361 acccgttctg ctacctccac aacccgacga gcggtagaca acaccagcgc catcagcgta
     3421 tcggcgacgg tttctgttaa tacggttggc gtgtgcatca gcagaatttt tcgggcggta
     3481 agcgcatcga catcaaaatt gtcatagccg acggagatcg ttgatgtggc acgcagtttc
     3541 ggcatttttt ccagcaatgc ggcatctaca ttctcgtttg aacccagtaa accttcagct
     3601 tctgcaaaaa ttgctgcatt ttgaacgacg gtttgtgggc tgaggtttgc cacctggtga
     3661 acggtgaaat gcgcttgcag gcgttgcagt aaatcatcag gtaaggcttt gtagaggata
     3721 acggacggct tcatgcttct ctccattcac tgataaggcg atcaccttag cctgaaatat
     3781 gatgaaggaa aactgaagaa tgccaggtga tttctgcatc acctggcatg aaagggatta
     3841 cagcgggctt aaggtaattt ctacacggcg gttttgcgcc ttaccttctg cggtgctgtt
     3901 gctggcgatt gggttagccg ggccaaggcc ctgagtacgg atgcggctgg cgtccacgcc
     3961 ctgggtgatc aacgcgctgg caacggaatc cgcacgctgc tgggagagac gcatgttcag
     4021 gtcgtgaccc cccgtgctgt cggtataacc aatcacgtta accgccgttt tcggatactc
     4081 tttcagcacc attgccacgc cggtcagggt gttagcgcct gccggtttca ggggagcgct
     4141 gctgctgtcg aaggtcacat tgttcggcat attgaggata atgttatccc cgctgcgggt
     4201 tacgctaaca ccagtgccgc gcattttgtc acgcagcttc gcttcctgca catccatgta
     4261 ataacccacg ccgccgccca gagctgcgcc tgctgctgcg ccaatcagcg cgcctttacc
     4321 gcgatctttc ttcgaagaag agagcgcacc aatacccgcg cccacgagag agcccagacc
     4381 tgcgccgata gcagatttgc ctgcttcgcg ttcgccggtg taagggttag ttgtgcagcc
     4441 agataccgcc agagcgccac tcactacggc ggcaagtaag ataaacacgt ttcttcattg
     4501 ttaatcctta ataacctttt tattctttgt cacgggttcc gtggcgggag attatgccgc
     4561 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4621 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4681 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4741 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4801 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4861 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4921 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4981 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     5041 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     5101 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     5161 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     5221 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     5281 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5341 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5401 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt
     5461 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5521 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5581 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5641 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5701 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5761 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5821 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5881 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5941 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     6001 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat tgttctgcct
     6061 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     6121 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     6181 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     6241 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6301 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6361 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6421 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6481 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6541 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag
     6601 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6661 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6721 tcccgtgggc agatccttgc cggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6781 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6841 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6901 ggtaatacga cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6961 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     7021 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     7081 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     7141 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     7201 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     7261 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7321 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7381 cttcccgaat catggtttat ttcccagata gcaacagcag ccaaccacat gatcattcac
     7441 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccacg
     7501 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7561 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7621 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7681 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7741 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7801 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaagcaga tcatttcgaa
     7861 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7921 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7981 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     8041 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     8101 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     8161 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     8221 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     8281 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8341 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8401 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8461 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8521 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8581 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8641 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8701 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8761 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8821 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8881 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8941 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     9001 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     9061 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     9121 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     9181 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     9241 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9301 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9361 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9421 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9481 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9541 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9601 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9661 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9721 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9781 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9841 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9901 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9961 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
    10021 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
    10081 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
    10141 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
    10201 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
    10261 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10321 cgtaaaattt gggccagtta acaggccctt tttactgctt tttgtaacgc taaagtctct
    10381 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10441 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10501 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10561 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10621 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10681 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10741 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10801 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10861 gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa
    10921 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10981 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    11041 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    11101 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    11161 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    11221 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    11281 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11341 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11401 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11461 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11521 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11581 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11641 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11701 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11761 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11821 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11881 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11941 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    12001 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    12061 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    12121 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    12181 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    12241 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12301 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt
    12361 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12421 caccgaccaa attcgaaaag cctgctcaac gagcaggttt ttttgcattt gtagctcatg
    12481 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12541 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12601 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12661 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12721 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12781 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatgtt
    12841 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12901 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12961 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    13021 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    13081 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    13141 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    13201 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    13261 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13321 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg
    13381 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13441 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13501 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13561 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13621 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13681 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13741 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13801 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13861 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13921 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13981 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    14041 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    14101 gccaagcact aacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    14161 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    14221 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
    14281 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14341 gccgcatggc ggagatgatt caggcagact gggcgaaagt gggcgtgcag gccaaaatcg
    14401 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14461 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14521 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14581 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14641 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14701 aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14761 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14821 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14881 tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14941 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    15001 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    15061 tgcgagtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    15121 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    15181 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    15241 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15301 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15361 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15421 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15481 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15541 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15601 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15661 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15721 ctgggtacta ttccgctagc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15781 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15841 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15901 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15961 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    16021 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    16081 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    16141 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    16201 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    16261 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16321 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16381 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16441 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16501 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagcctgctg
    16561 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16621 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16681 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16741 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16801 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16861 cctgagtggg gcactatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16921 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16981 ggtctgcgtg acgcgctcga tcccaaacta aagcagtaag aggttcgaga tggcgttatt
    17041 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    17101 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    17161 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    17221 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    17281 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17341 gtgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17401 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17461 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17521 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17581 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17641 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17701 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17761 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17821 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17881 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17941 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    18001 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    18061 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    18121 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    18181 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    18241 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18301 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18361 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18421 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18481 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18541 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18601 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18661 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18721 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18781 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18841 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18901 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18961 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    19021 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    19081 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    19141 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    19201 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    19261 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19321 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19381 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19441 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19501 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19561 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19621 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19681 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19741 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19801 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19861 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19921 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19981 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    20041 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    20101 agggttttta aataaatgtc ctgcactacc caggtgacag gataagcaat gatcgctgcg
    20161 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    20221 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    20281 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20341 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20401 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20461 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20521 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20581 aaaaagcaat cgtagaatca gcgtcaagcc ggaaggattc cggtgaggcg caatgttgcg
    20641 ggggcttaat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    20701 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
    20761 aaacgttgtc attacaacac aggcggctat atgacgctcg cagagctggg catggccttc
    20821 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
    20881 ctgaacaagc ggaagtagcg tgtcatgtgg gcgtcaggct gccgtaatgg caatttgcgc
    20941 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
    21001 gcaagcggat aatattctgc ggaatgcgca gcacagagtg aagatgagac ggattccgga
    21061 acagaaaaac agtcgtagaa tcagcgtcaa gccggaagga ttccggtgag gcgcaatgtt
    21121 gcgggggctt aatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca
    21181 ggtatgcacc tgacaacacc acgggggcca ttcttgcatc caaacaatac aagaatagcc
    21241 gcgaaacgtt gtcattacaa tacaggcggc tatatgacgt tcgcagaact gggcatggcc
    21301 ttctggcatg atttagcggc tccggtcatt gctggtattc ttgccagtat gatcgtgaac
    21361 tggctgaaca agcggaagta acgtgccatg cgggcgtcag gctgccgtaa tggcaatttg
    21421 cgcccggacc aggccgcagg ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg
    21481 gttgcaagcg gataatattc tgcggaatgc gcagcacaga gtgaagacgt gacggattcc
    21541 ggaacagaaa aacagtcgta gaatcagcgt caagccggaa gggttccggt gaggcgcaat
    21601 gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt
    21661 gcaggtatgc acctgacaac atcacggggg ccattcttga acctgaacaa ctcaagaata
    21721 gccgcgaaac gttgtcatta caacacaggc ggctatatga cgctcgcaga actgggcatg
    21781 gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg
    21841 aactggctga acaagcggaa gtaacgtgtc atgcgggcgt caggctgccg taatggcaat
    21901 ttgcgcccgg accaggccgc aggggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca
    21961 acgattgcac gcggataata ttctgcggaa tgcgcagcac agagtgaaga cgtgacggat
    22021 tccggaacag aaaaacagtc gtagaatcag cggcaagccg gaaaggttcc ggtgaggcgc
    22081 aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa acccccgcac
    22141 gttgcaggta tgcacctgac aacatcacgg gggctaatct tgaacccaaa caactcaaga
    22201 atagccgcga aacgttggca ttacaacaca ggcggctata tgacgctcgc agaactgggc
    22261 atggccttct ggcatgattt agcggctccg gtcattgctg gcattcttgc cagtatgatc
    22321 gtgaactggc tgaataagcg gaagtaacgt gtcatgcggg cgtcaggctg ccgtaatggc
    22381 aatttgcgcc cggatcaggc cgcagaggtt aaactctgcg gcctttttcg tgcttactgc
    22441 gggtaaggca cccagtcgcc gccgttcagg cgaacgtacg gtttatcttg gtattgaata
    22501 actactgcat ttgagttctc ggagaccggt gctgtttgtg gcaacccact ggtgagtttt
    22561 ttccagtcaa cattgtcttc ggtgaaaatc ttgccatcaa gaacgcgaac caccagatcg
    22621 gagatagcca ggaagctgct cggttggtca atgacaatcg gtgccccctg atgtggtgcc
    22681 ttcatgccga agaatttcac cccaacgggg acgtctgtga tagacgggct agggatatca
    22741 cgtaggccag atacctgcat tctgtcgccc ttcagcgcgc cgccgtgttc cggcaccacg
    22801 accaccatca ctttacgacc cgatttctcc agttcagtaa agaaggcgtc cagttcatca
    22861 aagaatttct gcgcccgcgc tttgtaatcc gctgttttgc tgacccccgg ataatggttg
    22921 ccgtcatgca gtggaagcgt gttgtagaac gtggcactac ggctgttttt atctttttcg
    22981 gtaacgtcca gccagcggtt aagcacggcg gtatcgtcat aaaccggcga accatcaaag
    23041 cccagcaaaa taaccggcag atttgtttga tccatcaatt cagtctgcat gccgccattt
    23101 tcgcgaactt ctttcaaaaa accgccgaac tggccgttat gtcccatcat caggtgctgg
    23161 gtaaagccca gtttcgaaag gttatcaaac agatagcagt cgttatttgc cggttgatac
    23221 agattagtgt gcgaagtctg cccgcagctg gcgcgcagta aacggatcgc cgccgggcca
    23281 ctgtaggagg tggcggagtt aaagttcttg aaatcaatat cgaaatgcga ccacagtgga
    23341 tgcgacatca acccggcggc ttctatatcc gaccaggaaa gcgaacagat gttaatcacc
    23401 agtagttcaa atggctgagc atcagcgggc agcgaagacg ggaaggtcga tttacgtttc
    23461 gcctccgcgt tatagaaatt attcagccag gcgttaaggt tcgccgttgt tggcggtgca
    23521 gtttgtgcgg gcatatcacc cactaccggt gcgccacccg tcgccgcaac ggttgccgct
    23581 gcgttaccac ccgtcgttgt tacagtggtc gtcggttgtc cggctggcca caaggagaaa
    23641 cttggtcccg ccaaggtaag tacgttcagc catagcagta tggcaaccac aaaaacggta
    23701 atgcgaatcc attgtgacag gaataaccag gccactaata aaacaaaaat ggccccaatc
    23761 atctgccagt taataaagcg tgtgacaagg tcgattaaat aatcggtact gaaccccgcc
    23821 acctgcgaac cctggctcat tatgctttcc gggccaggca accagatgtc atgccagaac
    23881 aaagcaaagc cgatcggcag ggcaatccag tggcgcaagc gatgcaggct gtaacgcgga
    23941 atgggcatca gcagaaacgc ggcaaacacc aaattgagga gcgggtggaa gttaagatat
    24001 cccgcccaca acaggccaaa cttaaccaga aaatagaagt tccagccgga aaggccgcgc
    24061 cagtattgcc agagggaaga aggcatggcg gtattttgcg taaattgagt cattttttgg
    24121 ttgccctggc tttttccgtg cggcgtaacg tcccggccgg tttaacataa cgaggtttag
    24181 caaagaacaa acgtaaggtg tcgcgaatgc gtcgcaaaga gtgccgcgcc agatagccca
    24241 gcgggaaaaa tatcagtgcg caaacgataa taatttcaat gatatcgctg atggtcatca
    24301 tgatgagcgc tccacagcat catctaacag tcgcatgggt tctggtattc gtcgccagtg
    24361 gcgaccatcg tgctcggcat tgatgaccgg tttagaactt tgcgttaaag gcagcggcat
    24421 tccccattgt tctggggcaa gcaggcgcat ctgcaccagc tcggcactga tttgatcatc
    24481 ttcaaaccag accatacggt ttgagaaaat gtcgccagta ggcaatggga aaatatgatt
    24541 caacgcggta tccagatcgt taatccgaca gaatgagaga aacagcacca gccgattacc
    24601 gccaatggtc atgatatcgc cggtacggtt agggcgacat agcgtcaggg cttgctcaac
    24661 gcggataccc ggtaccggac gtagggcaac cagaacgcct ttaccgtggg caggcaagag
    24721 agggttattc atcatgttgt tgacggcatt acagaacaca tcccacttct ggaaaccacg
    24781 cagtttgagc ggctgggtca ttgacagcaa ggtagtgata tcttccggta catagcgact
    24841 aaacttctgc ccttgcacgc tttcgatcat cgtcagacaa cgggagagtg gcgcattcca
    24901 cggaataacc atatttgcac cgcaggccaa taacaaacgt tcatcggtgg cgcgcaggct
    24961 agcggtattt tcccgcacga ggattttcat cgcactaccg cgctggcgac gcagggtatg
    25021 aatgctgcgg gccagtggct cgatttgcgc attttgctgt aaagaaaaga ccaccgtcgc
    25081 cgcctgagcg gtacgggctt cattgaacag gacttcgtta ttgttgaaca gttgccagtg
    25141 ttccgatagc ggcggcgcac cttccagtac agcaacatta ctcagaatgc gtttttcgtc
    25201 gctgcgtggt tggatctccg cctcttcgct ttgaactaat gtccagatac cattttgttg
    25261 ctgaacgcta agctgctgac gggcgctgac ccctttttcg ttgcaccaga aggcaatatc
    25321 cagcaaatgt tggtcaccct gaaaacgcaa actggcaaga ccaaaaagtg aacggtattc
    25381 ctcaagcaat aatgaaaatt gtttatcgtt attatttccg ggattaatta ccaaaagcga
    25441 acaatggttt aacctgctcc atttattcat tttatccaac catgagcgaa gccgctcggc
    25501 aggaatattt tgccatgcgt tatttgcgca aacaagaata aaaaggtaat tatggggatc
    25561 aatagaacac tgcaaatcac gggccaaata gtatagacct ttttcatgat ttagcattga
    25621 aaataatttt attttttccg gcccttgaga atcatctaat tgaaagattt tcgccggatc
    25681 gctgtccatg ctaatgaccg cgacgtgtgc ggtttcagcc tgggatgcaa ttgtttgatt
    25741 cgccagactg atagcatctt catggcgatc gacgttaaac caccagacgc cgcctgctgg
    25801 catatgtcgc agctcatccc ataatgatga gataccgata gagaatacag ggtccacaat
    25861 gtccctcatg tcaccatttt gtctgattat cagtttacta gcgaaagcta agaaataaac
    25921 ctaccattga aattaaaact tatcagattt agcatgtgaa tcaatttaga aggtcacaag
    25981 acgtgtgatt ttagtctttt cgtttttaac tttttctgtt gcgtaacgga aagtcaaaaa
    26041 gtgagcaaat tcccgtctcg ccgtaaacaa taaaccggcg aaatgctcac ggttagaatt
    26101 gtctcatgaa tggtacggtt atttcatagg gatcaagcaa aatgaataac aatgaaccag
    26161 atactctgcc tgatcccgcg ataggctata tcttccagaa tgatattgtg gcgttaaagc
    26221 aggcattttc actgcctgat attgattatg ccgatatttc ccaacgcgaa cagttggccg
    26281 cggcattaaa acgctggccg ttgctggcag agtttgcgca acaaaagtag gggattggtg
    26341 aatggccgta ctgggattgc agggggtgcg gggaggcgtg gggacaacaa ccatcaccgc
    26401 cgcattagcc tggtcattac aaatgttggg agaaaatgtc ctggtggtcg atgcctgccc
    26461 ggacaacttg ttgcgcctgt catttaatgt tgattttacc caccgtcagg gctgggccag
    26521 agcgatgctg gatggccagg actggcgtga cgctgggttg tgctacacct cgcagctcga
    26581 tttgctgcct tttggtcagt tatccattga agaacaagaa aatccacagc actggcaaac
    26641 ccggctgagc gatatttgct ccggcttaca gcaactaaaa gccagcgggc gttaccagtg
    26701 gattttaatc gacttaccgc gtgatgcctc gcagataacc caccagctgc tgagtttgtg
    26761 cgagcactcg ctggcaatcg tcaatgtgga tgccaactgc catatccgac tgcatcagca
    26821 agcgctgccg gatggcgcac atattttgat taatgacttc cgtattggca gtcaggttca
    26881 ggacgatatt taccagcttt ggttgcaaag ccagcgccga ttactgccga tgctcattca
    26941 tcgtgatgaa gcgatggctg aatgcctggc ggctaagcaa ccagtaggtg aatatcgcag
    27001 tgatgcgctg gcggctgaag agatactgac gctggcgaac tggtgcctgt tgaactactc
    27061 cgggctgaaa acgccagtcg ggagtgcatc atgagtatcc tgacccggtg gttgcttatc
    27121 ccgccggtca acgcgcggct tatcgggcgt tatcgcgatt atcgtcgtca cggtgcgtcg
    27181 gctttcagcg cgacgctcgg ctgtttctgg atgatcctgg cctggatttt tattccgctg
    27241 gagcacccgc gctggcagcg tattcgcgca gaacataaaa acctgtatac gcatatcaac
    27301 gcctcgcgtc cgcgtccgct ggacccggtc cgttatctca ttcaaacatg ctggttattg
    27361 atcggtgcat cgcgcaaaga aacgccgaaa ccgcgcagcg ggcattttca ggtctgcaaa
    27421 atattcgtgg acgttaccat caatggatga acgagctgcc tgagcgcgtt agccataaaa
    27481 cacagcatct ggatgaaaaa aaagagctcg gtcatttgag tgccggggcg cggcggttga
    27541 tcctcggtat catcgtcacc ttctcgctga ttctggcgtt aatctgcgtt actcagccgt
    27601 ttaacccgct ggcgcagttt atcttcctga tgctgctgtg gggggtagcg ctgatcgtac
    27661 ggcggatgcc ggggcgcttc tcggcgctaa tgttgattgt gctgtcgctg accgtttctt
    27721 gccgttatat ctggtggcgt tacacctcta cgctgaactg ggacgatccg gtcagcctgg
    27781 tgtgcgggct tattctgctc ttcgctgaaa cgtacgcgtg gattgtgctg gtgctcggct
    27841 acttccaggt agtatggccg ctgaatcgtc agccggtgcc attgccgaag gatatgtcgc
    27901 tgtggccgtc agtggatatc tttgtcccga cttacaacga agatctcaat gtggtgaaaa
    27961 ataccattta cgcctcgctg ggtatcgact ggccgaaaga caaactgaac atctggatcc
    28021 tcgatgacgg cggcagggaa gagtttcgcc agtttgcgca aaacgtgggg gtgaaatata
    28081 tcgcccgcac cactcatgaa catgcgaaag cgggcaacat caacaatgcg ctgaaatatg
    28141 ccaaaggcga gttcgtgtcg attttcgact gcgaccacgt accaacgcga tcgttcctgc
    28201 aaatgaccat gggctggttc ctgaaagaga aacagctggc gatgatgcag actccacacc
    28261 acttcttctc gccggacccg tttgaacgca acctgggacg ttttcgtaaa acgccgaacg
    28321 aaggcacgct gttctatggt ctggtgcagg atggcaacga tatgtgggac gccactttct
    28381 tctgcggttc ctgtgcggtg attcgtcgta agccgctgga tgaaattggc ggcattgctg
    28441 tcgaaaccgt gactgaagat gcgcatactt ctctgcgttt gcaccgtcgt ggctatacct
    28501 ccgcgtatat gcgtattccg caggcggcgg ggctggcgac cgaaagtctg tcggcgcata
    28561 tcggtcagcg tattcgctgg gcgcgcggga tggtataaat cttccgtctc gataacccgc
    28621 tcaccggtaa aggactgaag tttgctcagc ggctgtgcta cgtcaacgcc atgttccact
    28681 tcttgtcggg cattccacgg ctgatcttcc tgactgcgcc gctggcgttc ctgctgcttc
    28741 atgcctacat catctatgcg ccagcgttga tgatcgccct attcgtgctg ccgcatatga
    28801 tccatgccag cctgaccaac tcgaagatcc agggcaaata tcgccactct ttctggagtg
    28861 aaatctacga aacggtgctg gcgtggtata tcgcaccacc gacgctggtg gcgctgatta
    28921 acccgcacaa aggcaaattt aacgtcaccg ccaaaggtgg actggtggaa gaagagtacg
    28981 tcgactgggt gatctcgcgg ccctacatct tccttgttct actcaacctg gtgggcgttg
    29041 cggtaggcat ctggcgctac ttctatggcc cgccaaccga gatgctcacc gtggtcgtca
    29101 gtatggtgtg ggtgttctac aacctgattg ttcttggcgg cgcagttgcg gtatcggtag
    29161 aaagcaaaca ggtacgccga tcgaaccgcg tggagatgac gatgcccgcg gcaattgccc
    29221 gcgaagatgg tcacctcttc tcgtgtaccg ttcaggattt ctccgacggt ggtttgggga
    29281 tcaagatcaa cggtcaggcg cagattctgg aagggcagaa agtgaatctg ttgcttaaac
    29341 gcggtcagca ggaatacgtc ttcccgaccc aggtggcgcg cgtgatgggt aatgaagttg
    29401 ggctgaaatt aatgccgctc accacccagc aacatatcga ttttgtgcag tgtacgtttg
    29461 cccgtgcgga tacatgggcg ctctggcagg acagctaccc ggaagataag ccgctggaaa
    29521 gtctgctgga tattctgaag ctcggcttcc gtggctaccg ccatctggcg gagtttgcgc
    29581 cttcttcggt gaagggcata ttccgtgtgc tgacttctct ggtttcctgg gttgtatcgt
    29641 ttattccgcg ccgcccggag cggagcgaaa cggcacaacc atcggatcag gctttggctc
    29701 aacaatgatg ataacgcgat gaaaagaaaa ctattctgga tttgtgcagt ggctatgggg
    29761 atgagtgcgt tcccctcttt catgacgcag gcgacgccag caacgcaacc actgatcaat
    29821 gctgagccag ctgtagccgc ccagacggaa caaaatccgc aggtggggca agtgatgtca
    29881 ggcgagcagg gagctgatgc caccatcgtg gcgcagaacg gtccttcgcg tgatgtgaag
    29941 ctgacctttg cgcaaatcgc gccgccgccg ggcagcatgg tgctacgtgg tattaacccg
    30001 aacggcagca ttgagtttgg tatgcgcagc gatgaagtgg tgacgaaggc gatgctcaac
    30061 ttcgaataca cgccatcgcc atcgttactg cctgtccagt cgcagttaaa ggtttatctc
    30121 aatgatgagc tgatgggcgt gctgccagtg accaaagaac agttgggtaa aaaaacgctg
    30181 gcgcaaatgc ccattaatcc actgtttatt accgacttca accgtgtgcg gctggagttt
    30241 gtcggccatt atcaggacgt gtgcgaaaac ccggccagca ccacgctttg gctggatgtt
    30301 gggcggagca gtggactgga tctgacctat cagaccctga atgtgaagaa tgacctgtca
    30361 cacttcccgg tgccattctt tgacccgcgc gataaccgta ctaacacctt gccgatggtc
    30421 tttgcgggtg cgccggatgt tgggctgcaa caagcatctg ccattgtcgc ctcgtggttt
    30481 ggttcgcgtt ctggctggcg tgggcagaac ttcccggtgc tctataacca attgccggat
    30541 cgcaatgcca ttgtctttgc aaccaacgac aaacggccgg acttcctgcg cgatcatccg
    30601 gcggtaaaag ccccggtgat tgagatgatt aaccatccgc agaatcctta cgtcaaactg
    30661 ctggtggtgt ttggtcgtga cgacaaagac ctgttgcagg cagcgaaagg tatcgctcag
    30721 ggtaacattc tgttccgtgg tgaaagcgtg gtagtgaatg aagtgaaacc gctgctaccg
    30781 cgtaagccgt acgatgcgcc gaactgggta cgtaccgatc gtccggtcac atttggcgaa
    30841 ctgaaaacct atgaagaaca gttacaatcc agcggccttg agccagcagc gattaacgtt
    30901 tcgctaaacc tgccgccgga tctctacctg atgcgcagta ccggcattga tatggatatt
    30961 aattaccgct acaccatgcc gccggtgaaa gacagttcgc ggatggatat cagcctgaat
    31021 aaccagttcc tgcaatcctt caacctgagc agcaaacagg aggcgaaccg tctgctgctg
    31081 cgtattccgg tattacaagg tttgctggat ggcaaaacag atgtctctat tccggcgctg
    31141 aaactgggcg cgaccaacca gctgcgcttc gactttgagt atatgaaccc gatgccaggc
    31201 ggttcggtag ataactgtat taccttccag ccggtgcaga atcatgtggt gattggtgac
    31261 gattccacca tcgacttctc gaagtattac cacttcatcc cgatgccgga tctacgcgcc
    31321 tttgctaacg cgggcttccc attcagccgg atggcggatc tgtcgcaaac catcaccgtg
    31381 atgccgaaag cgcctaacga agcacagatg gaaacgttgc tgaatactgt tggttttatc
    31441 ggcgcacaga cgggcttccc ggcgattaat ctgacggtga ccgatgatgg cagcaccatt
    31501 cagggcaaag atgccgacat catgatcatc ggtggtatcc cggacaaact gaaagacgat
    31561 aagcagatcg acctattggt gcaggcgacc gaaagctggg tgaaaacacc gatgcgccag
    31621 accccgttcc ccggcattgt gccggacgag agcgatcgcg cggcagaaac ccagtcaact
    31681 ctgacctctt ccggtgcgat ggcggcggtg attggcttcc agtcgccgta taacgaccag
    31741 cgcagcgtga ttgcgctgtt ggcagatagc ccacgcggtt atgaaatgct taacgatgcg
    31801 gtgaacgata gcggcaaacg cgccaccatg ttcggttcgg tcgcggtgat ccgcgagtcc
    31861 ggtatcaaca gcctacgtgt tggcgacgtt tattacgtag gtcatctgcc gtggttcgag
    31921 cgcttgtggt atgcgctggc aaaccatccg attctgctgg cggtgctggc gactatcagt
    31981 gtgatattgc tggcatgggt actgtggcgt ctgctgcgaa ttattagtag tcgtcgtctt
    32041 aacccggata acgagtaatt gaagatgaat gtgttgcgta gtggaatcgt gacgatgctg
    32101 ttgttggctg cctttagtgt tcaggcagcc tgtacctggc ctgcctggga gcagtttaaa
    32161 aaggattaca tcagtcagga agggcgcgtc atcgacccca gcgacgcgcg caaaatcacc
    32221 acctccgaag ggcaaagtta cggtatgttc tttgccctgg cggctaacga ccgtgcagct
    32281 ttcgataata ttctcgactg gacgcagaac aatctcgctc agggttcttt aaaagaacgt
    32341 ttgcccgcct ggctgtgggg caagaaagag aacagtaagt gggaagtgct ggacagcaat
    32401 tcggcctccg atggtgatgt ctggatggcc tggtcgttgc tggaggcggg gcgtttgtgg
    32461 aaagagcagc gttataccga catcggcagc gcgttgctaa aacgtatcgc gcgggaggaa
    32521 gtggtgacgg tgcctgggct gggttccatg ttgttaccgg gcaaagtggg ttttgctgag
    32581 gataacagct ggcgttttaa ccccagctac atgccgccga cgctggcgca gtatttcacc
    32641 cgctttggcg cgccgtggac cacgctgcgc gaaaccaatc aacatttatt gctggaaacc
    32701 gccccgaaag gcttttcgcc agactgggtg cgctatgaga aagacaaagg ctggcagcta
    32761 aaagccgaaa aaacattgat cagcagctac gacgctatcc gcgtttacat gtgggtaggc
    32821 atgatgcctg acagcgatcc gcaaaaagcg cggatgctca accggtttaa accgatggcg
    32881 acattcactg agaaaaacgg ttatccgccg gaaaaagtgg atgtggctac ggggaaagcg
    32941 cagggtaaag gaccggtcgg tttttctgcc gccatgctgc cttttttaca aaaccgcgat
    33001 gcgcaggcag ttcagcgcca gcgcgtggcc gataactttc ccggcagcga tacctattac
    33061 aactatgtgc tgaccctgtt tggacaaggc tgggatcaac accgtttccg cttctcgaca
    33121 aaaggtgagt tattacctga ctggggccag gaatgcgcaa attcacacta aacatattca
    33181 cgctttccct cggtctggcc gtcatgccga tggtcgaggc agcaccaacc atcagcagac
    33241 cctgcggatg gacagcggca acactaatgc cgtgcgcggg ctggcgaata tttaccgcca
    33301 gcagtcgcca gaaaaagctg aagcgtttat cgcctcgctc tctgccagcc agcggcgcag
    33361 cattgatgat atcgaacgca gcctgcaaaa cgaccgtctg gcacagcagg cagaggtact
    33421 ggaaaaccag ggcaaatggg cgcaggcggc agcacttcag cggcaacgac tggcgctggg
    33481 ccccggcagc gtatggatta cttaccgact ttcgcaggat ctctggcagg ccggacaacg
    33541 cagccaggcc gatacgttaa tgcgcaatct ggcgcagcag aagccgaaca acccggagca
    33601 ggtttacgct tacgggctgt acctctcagg tcacaaccag gacagagcgg cgctggcgca
    33661 tatcaacagc ctgccgcgcg cgcagtggaa cagcaatatt caggagctgg ttaatcgact
    33721 gcaaagcgat caggtgctgg aaaccgctaa tcgcctgcga gaaagcggca aagaggctga
    33781 agcggaagcg atgctgcgtc agcaaccacc ttccacgcgt attgacctca cgctggctga
    33841 ctgggcgtaa caacgacgtg attacaccgc cgcccgcact gcatatcaga atgtcctgac
    33901 gcgggagcca actaacgccg atgccattct tggtctgacg gaagtggata ttgctgccgg
    33961 tgacaaagcg gcggcacgta gccagctggc gaaactgcct gccaccgata acacctcgct
    34021 gaacactcag cggcgcgtgg cgctggcaca ggcgcagctt ggagataccg cagcggcgca
    34081 gcagacgttt aataagttga tcccgcaggc aaaatctcag ccaccgtcga tggaaagcgc
    34141 gatggtactg cgcgacggtg cgaagtttga agcgcaggcg ggcgatccaa agcaggcgct
    34201 ggaaacctac aaagacgcca tggtcgcatc cggtgtgact acgacgcgtc cgcaggataa
    34261 cgacaccttt acccgactga cccgtaacga cgagaaagat gactggctga aacgcggcgt
    34321 gcgcagcgat gcggcggacc tctatcgcca gcaggatctt aacgtcaccc ttgagcacga
    34381 ttactggggt tcgagcggca ccggtggtta ctccgatctg aaagcgcaca ctaccatgtt
    34441 gcaggtggat gcgccgtatt ctgacgggcg gatgttcttt cgcagtgatt tcgtcaatat
    34501 gaacgtcggc agtttctcca ctaatgccga tggcaaatgg gatgacaact ggggcacctg
    34561 tacattacag gactgtagcg gcaaccgcag ccagtcggat tccggtgcca gcgtggcggt
    34621 cggctggcga aatgacgtct ggagctggga tatcggtacc acgccgatgg gcttcaacgt
    34681 ggtggatgtg gtcggcggca tcagttacag cgatgatatc gggccgctgg gttacaccgt
    34741 taacgcccac cgtcggccca tctccagttc tttgctggcc tttggtgggc aaaaagactc
    34801 cccgagcaat accgggaaaa aatggggtgg cgtacgtgcc gacggtgtgg ggctaagtct
    34861 gagctacgat aaaggtgaag taaacggcgt ctgggcatcg cttagtggcg accagttaac
    34921 cggtaaaaat gtcgaagata actggcgcgt gcgctggatg acgggctatt actataaggt
    34981 cattaaccag aacaatcgcc gcgtcacaat cggcctgaac aacatgatct ggcattacga
    35041 caaagatctg agtggctact cactcggtca gggcggttac tacagtccgc aggaatacct
    35101 gtcgtttgcc ataccggtga tgtggcggga gcgcacggaa aactggtcgt gggagctggg
    35161 ggcgtctggc tcgtggtcgc attcacgcac caaaaccatg ccgcgttatc cgctgatgaa
    35221 tctgatcccg accgactggc aggaagaagc tgcgcggcaa tccaacgatg gcggcagcag
    35281 tcagggcttc ggctacacgg cgcgggcatt acttgaacga cgtgttactt ccaactggtt
    35341 tgttggtacg gcaattgata tccagcaggc gaaagattac gcacccagcc atttcctgct
    35401 ctacgtacgt tattccgccg ccggatggca gggtgacatg gatttaccgc cgcagccgct
    35461 gataccttac gccgactggt aagttttcag atagcgcctc tcttaatgcc gctgcgatcg
    35521 ggtatactcg ggcggcaatc tgggatttcc ggggggagac aatttgcgcg taagtcgctc
    35581 gttaacaatc aagcagatgg caatggtggc agccgttgtc ctggtgttcg tttttatttt
    35641 ttgcaccgtt ttgctgttcc atctggtcca gcagaatcgc tataacacgg ctacgcaact
    35701 ggaaagcatt gctcgctctg tccgcgaacc cttatcttca gctattttga aaggcgatat
    35761 tcccgaagcg gaagctattc ttgccagcat taaaccggca ggcgtggtca gccgtgccga
    35821 tgtagtgctg cctaaccagt tccaggcgct gcgtaaaagt tttattccag agcgtccggt
    35881 gccggtaatg gttactcgcc tgtttgagct accggttcaa atctcgctgg gcgtctactc
    35941 gctcgaacgt ccggcaaatc cgcagccaat agcctatctg gtgctacagg cggattcctt
    36001 ccgtatgtat aagttcgtga tgagcactct ctcaacgtta gtgaccattt acttactttt
    36061 gtcgcttatc ctgaccgtcg ccatcagctg gtgcattaac cgcctgattt tgcatccgtt
    36121 acgcaatatt gctcgcgaac ttaacgccat cccagcccag gagcttgttg gtcaccaact
    36181 ggcattaccg cgtctgcatc aggacgatga aatcggtatg ttggtgcgca gttacaacct
    36241 caaccagcaa ttgctgcagc gccattatga agaacagaac gaaaatgcga tgcgcttccc
    36301 ggtgtcggat ttgccgaaca aagccttgct gatggagatg ctggagcagg ttgtcgcgcg
    36361 taaacaaacc accgcgctga tgatcatcac ctgtgaaacc ctgcgtgata ctgcgggcgt
    36421 gctgaaagag gcgcaacgag aaattctgct gctgacgctg gtggaaaaac tcaaatcggt
    36481 actgtcgcca cgtatgatcc tcgcgcagat tagcggttat gactttgctg tcattgccaa
    36541 cggtgtacag gaaccgtggc acgcaatcac tttgggtcag caagtactca ctatcatgag
    36601 cgagcgcctg ccgattgaac gtattcaact ccgtccgcac tgtagcattg gcgtggcgat
    36661 gttctacggc gatctcaccg ccgaacagct ttacagtcgc gctatttctg cggcatttac
    36721 cgctcgccat aaaggcaaga atcagattca gttctttgat ccgcagcaga tggaagcagc
    36781 ccagaagcgg ttgacggaag agagcgatat ccttaatgca ctggaaaatc atcagtttgc
    36841 aatttggtta cagccacagg tcgagatgac cagcggtaaa ctggtcagtg cggaagtgtt
    36901 actgcgtatc cagcaaccgg atggcagttg ggacctgccg gatggcttaa tcgatcgcat
    36961 tgagtgctgt gggctgatgg ttaccgtcgg tcactgggtg ctggaagagt cctgtcgatt
    37021 gcttgcagcc tggcaagagc gcggcattat gctgcccttg tcggtaaact tctctgcgct
    37081 gcaactgatg cacccgaata tggtggcgga tatgctggaa ctgttaaccc gctatcgcat
    37141 tcagccggga acactgattc tggaagtgac agaaagccga cgtattgacg accctcatgc
    37201 tgcggtggca atcctccgtc cgctgcgcaa tgccggagtt cgggtggcgc tggatgattt
    37261 cggcatgggc tacgcagggc tgcgtcagct gcagcatatg aaatcgttgc caatcgacgt
    37321 actgaaaatc gacaaaatgt ttgttgaagg cttgccggaa gatagcagca tgattgctgc
    37381 aattatcatg ctggcgcaga gcctgaactt acaaatgatt gccgaaggcg tggagactga
    37441 agcacaacga gactggctgg caaaagcggg cgttggtatt gcccagggct tcctttttgc
    37501 tcgcccactc cctattgaaa tcttcgaaga gagttacctg gaagaaaagt agctacccca
    37561 aactgattac aaaactttaa aaagtgctgg tttgtgcgag ccagctcaaa ctttttaacc
    37621 tttttgtttc aattatgatc caggtacatt tctgtgatgt tgtctgggtg ttattttaag
    37681 gccgcaggta ccccccataa ccttacaaga cctgtggttt tactaaagga caccctatga
    37741 aaacctctct gtttaaaagc ctttactttc aggtcctgac agcgatagcc attggtattc
    37801 tccttggcca tttctatcct gaaataggcg agcaaatgaa accgcttggc gacggcttcg
    37861 ttaagctcat taagatgatc atcgctcctg tcatcttttg taccgtcgta acgggcattg
    37921 cgggcatgga aagcatgaag gcggtcggtc gtaccggcgc agtcgcactg ctttactttg
    37981 aaattgtcag taccatcgcg ctgattattg gtcttatcat cgttaacgtc gtgcagcctg
    38041 gtgccggaat gaacgtcgat ccggcaacgc ttgatgcgaa agcggtagcg gtttacgccg
    38101 atcaggcgaa agaccagggc attgtcgcct tcattatgga tgtcatcccg gcgagcgtca
    38161 ttggcgcatt tgccagcggt aacattctgc aggtgctgct gtttgccgta ctgtttggtt
    38221 ttgcgctcca ccgtctgggc agcaaaggcc aactgatttt taacgtgatc gaaagtttct
    38281 cgcaggtcat cttcggcatc atcaatatga tcatgcgtct ggcacctatt ggtgcgttcg
    38341 gggcaatggc gtttaccatc ggtaaatacg gcgtcggcac actggtgcaa ctggggcagc
    38401 tgattatctg tttctacatt acctgtatcc tgtttgtggt gctggtattg ggttcaatcg
    38461 ctaaagcgac tggtttcagt atcttcaaat ttatccgcta catccgtgaa gaactgctga
    38521 ttgtactggg gacttcatct tccgagtcgg cgctgccgcg tatgctcgac aagatggaga
    38581 aactcggctg ccgtaaatcg gtggtggggc tggttatccc gacaggctac tcgtttaacc
    38641 ttgatggcac atcgatatac ctgacaatgg cggcggtgtt tatcgcccag gccactaaca
    38701 gtcagatgga tatcgtccac caaatcacgc tgttaatcgt gttgctgctt tcttctaaag
    38761 gggcggcagg ggtaacgggt agtggcttta tcgtgctggc ggcgacgctc tctgcggtgg
    38821 gccatttgcc ggtagcgggt ctggcgctga tcctcggtat cgaccgcttt atgtcagaag
    38881 ctcgtgcgct aactaacctg gtcggtaacg gcgtagcgac cattgtcgtt gctaagtggg
    38941 tgaaagaact ggaccacaaa aaactggacg atgtgctgaa taatcgtgcg ccggatggca
    39001 aaacgcacga attatcctct taatctcacc acttatgccc gcactccctt ccccgagtgc
    39061 gggtgtttgc gcataattga cccctgtgcc tgaacttacc acctgggtga gttcaggttt
    39121 atttcacttc ttccgtgaca ttttcatgtt cttgcggtct aacacgaagt gtttttaacg
    39181 tcatttagac tgaccgacaa cgggcagttt ttttattacc aggatagttg atcaggggtt
    39241 cacatgcagg gcataaaaat tcgactttta gcgggcggtt tgctgatgat ggccactgct
    39301 ggctatgtgc aggcagatgc gctccagcct gatccagcat ggcaacaggg gacgctttcc
    39361 aacggtttac agtggcaagt gctgaccacc ccccagcgtc ccagcgatcg tgttgaaatt
    39421 cgcctgctgg ttaataccgg ttcgctcgcc gaaagtacac aacagagcgg ttacagtcac
    39481 gccatccctc gtattgcgct aacgcaaagc ggtggccttg acgcagcaca ggcgcgttca
    39541 ttgtggcagc aggggatcga ccctaaacgc ccgatgccgc cggtaattgt ctcttatgac
    39601 accacgctgt ttaatctgag tttgcccaat aaccgtaacg atttgctgaa agaagcgctc
    39661 tcttatctgg caaatgccac tggcaaattg accatcacac cagaaaccat caaccacgcg
    39721 ctgcaaagtc aggacatggt ggcaacctgg cctgccgata ctaaagaggg ctggtggcgc
    39781 tatcgtctga aagggtcaac cttgttaggt cacgatcctg ccgatccgct gaaacaaccc
    39841 gttgaagcgg aaaaaattaa agatttctat cagaaatggt acaccccgga tgcaatgacg
    39901 ctactggtgg tgggaaacgt ggatgcgcgc tcggttgtcg accaaatcaa caaaacgttt
    39961 ggcgaactga aaggcaaacg tgaaacgcca gctccggtgc cgacgctttc tccgctgcgt
    40021 gcggaagcgg tgagtattat gactgacgcg gtgcgtcagg accggttatc tatcatgtgg
    40081 gatacgccgt ggcagccgat tcgtgaatca gccgcactgc tgcgctactg gcgtgcggac
    40141 ctggcccgcg aggcgctgtt ctggcatgtt cagcaagcgt taagcgccag tgacagcaaa
    40201 gacatcggtc ttggatttga ctgccgtgtg ctgtatctgc gtgcgcagtg tgccatcaac
    40261 atcgaatcac caaacgacaa gctgaacagc aaccttaatc tggtggcgcg tgaactggcg
    40321 aaggttcgcg ataaaggtct gccggaagaa gagttcaatg cgttagtggc gcaaaagaaa
    40381 ctggagctgc agaaactgtt tgccgcctat gcacgggctg ataccgatat tctgatgggc
    40441 cagcggatgc gttcgttgca aaatcaggtc gtcgatatcg cgccggagca gtatcagaaa
    40501 ctgcggcagg atttccttaa cagcctgact gtggagatgt taaatcagga tctgcgtcag
    40561 cagttgtcga atgatatggc gttaatactg ctgcagccga aaggcgagcc ggaatttaac
    40621 atgaaagcgt tgcaggcggc ctgggatcaa atcatggccc catctaccgc cgctgcgacc
    40681 acctctgtcg ccacggatga cgtacatcct gaagtgacgg atattccacc tgcacagtaa
    40741 tgcctgatgg tgatgagtta gcacaaccat caggtattta tggatgagct ggatagaggg
    40801 gtaacacttt atccagcctt ttgcatatgc tgcgtttacg ctggcatcgc ctcacgcggg
    40861 ataatcgcgc cgcgatactg aataacggta cttgcggtca ggtgcccgcg tttcgccgcg
    40921 ttttccgcac tgccgcccgt caaacgtacc gccagataac cggcgctgaa agagtcgcca
    40981 gctgcggtgg tatcgatcac tttttctttc ggcagtttca ccgccggaac atcaactaac
    41041 gcttcaccag caatggacac caggcaagaa tccgccccgc gtttcaccac cacttctttc
    41101 acgcccgcgt tatgggtgcg cgcaatgacg tcttccaccg gctgttgacc ccacagcgcg
    41161 tcttcgtcgt ccagcgtcag gaaggcgata tccgtgcatt ccagcatttg ctggtacacc
    41221 tgctgtgtct cttctttgct ggcccacagg cgcggacgat agttattgtc gaaaatcact
    41281 tttccgcctt tggcgcggca ttcgcgcagc agggaaagca gcttttcgcg gctggtcggg
    41341 cttaagatcg ccaggctaat cccgctcagg tagagataat cgaaattcgc cagctcttcg
    41401 caaatcgccg cagactgctc gctcgccagc cagaatttgg cggcggcttc gttacgccag
    41461 tagtagaacg tacgttcgcc ggtgctgtcg gtttcaatgt agtaaaggcc cggaagacgg
    41521 ttttccatcc gttgggtcag ggaagtatca acgttctcgc cgtgccaggc gtccagcatc
    41581 tgctggctaa aactgtccgt tcccagcgcc gttacgtaat gaacggttaa tgccgcagga
    41641 tcgacctgac gggcgatata gacggaagtg ttcagggtat cgccgccgaa accgcgctta
    41701 acgtccgcgc ctttctcgga aagctcaatc atgcattcgc caatcacggc aatctttttg
    41761 gacatagtcg tgaacctgat ctgtaaaaaa ttagcgttag tttgcgctgt ggtggtttgc
    41821 tggtcaacca tattaaaaca gtgttccatt atttttttga gccagaacgt attcctgaaa
    41881 gattccgttg tggagtgagg aaatttaccc cggccaggcg gtaaagttct gcccttacgc
    41941 gccgataatc tttgtcgagt ccgggcagca tcacttttaa acacaggaca tctttgatga
    42001 taaggcaggt tatccagcga ataagcaacc ctgaagcaag catcgagagc ttgcaggaac
    42061 ggcgtttttg gttgcagtgt gagcgtgctt acacctggca gccgatctat caaacatgcg
    42121 ggcggttaat ggccgtggag ctattaacgg tggtcacgca tcccttgaac ccttcgcaac
    42181 gcctgccgcc ggatcgctat tttactgaaa tcaccgtcag ccatcggatg gaggttgtga
    42241 aagagcagat tgatttgctg gcgcaaaaag ccgacttctt tgtagagcac ggcctgctgg
    42301 catcggtcaa tattgatggc cctacgctca tcgccctgcg tcagcaacca aaaatcctgc
    42361 gccagattga gcgtcttccc tggctgcgtt tcgaactggt ggagcatatc cgtctgccga
    42421 aagattcaac ctttgcctcg atgtgtgaat ttggcccgct gtggctggat gattttggta
    42481 ccgggatggc aaatttctct gcactaagtg aagtgcgtta tgactacatc aaaatcgcgc
    42541 gagaactgtt tgtgatgctg cgtcagtcgc cggaaggccg cacactcttt tctcagcttt
    42601 tacatctaat gaatcgctat tgtcgcgggg tgattgtcga gggcgtagaa acaccggaag
    42661 agtggcgtga tgttcagaac tcgcccgcat tcgccgcaca aggctggttt ctttcacgcc
    42721 cggcaccgat ggaaacgctg aatacggcgg ttctggcgct ataagctgcc tcattttccg
    42781 ccgggctgga ctatctttag gactggcaca ggaagaatat gaggaagcga acgatgagca
    42841 aggcaggcaa aataaccgct gcgatttcag gggctttctt gttgttgatt gtcgtggcga
    42901 tcattttgat tgcaactttt gactggaatc gactcaaacc gaccatcaac cagaaagtct
    42961 ctgcggagtt gaatcgtccg ttcgctatcc gtggcaatct gggcgtggtg tgggagcggc
    43021 aaaaacaaga aactggctgg cgcagctggg tgccgtggcc tcatgtacat gcagaagaca
    43081 tcattcttgg taatccaccg gatattcccg aagtcacgat ggtgcatttg ccgcgagtag
    43141 aggcaacgct ggcaccgctg gcgctgctga ccaaaacggt ctggctgccg tggatcaagc
    43201 tcgaaaagcc cgacgcgcgc ctgattcgcc tctctgaaaa gaacaataac tggacgttta
    43261 atcttgccaa cgatgacaac aaagacgcga atgcaaagcc gtcggcatgg tcgtttcggc
    43321 tggataatat tcttttcgat caagggcgga tcgccattga tgacaaagta agcaaagcgg
    43381 atctggaaat ttttgttgat ccgttaggca agccgctgcc attcagcgaa gttactggat
    43441 cgaaaggtaa agcggataaa gaaaaggtgg gcgattacat ttttggcctg aaggcgcagg
    43501 gacgttataa cggtgaaccg ctcacgggta cgggaaaaat aggcggtatg ctggcgctgc
    43561 gtggcgaagg gacgccgttt ccggtacagg ctgatttccg ttcaggtaat acccgtgttg
    43621 cttttgatgg cgtcgtgaat gacccaatga agatgggcgg tgtcgattta cggcttaaat
    43681 tttctggcga ttcactgggt gatctctatg aactgacggg cgttctgctg cccgataccc
    43741 cgccgtttga aacggatggt cggctggtag cgaaaatcga cactgaaaaa tcgtcggtct
    43801 ttgattatcg cggttttaat gggcgaattg gcgatagcga tatccacggt tctctggtct
    43861 acaccaccgg aaagccacga ccaaaactgg aaggtgatgt cgagtcgcgg caattgcggc
    43921 tggcggacct gggaccgttg attggcgttg attccgggaa aggggcagaa aagtcgaaac
    43981 ggtctgaaca gaagaagggc gaaaaaagcg ttcagcctgc gggcaaagtg ctgccttatg
    44041 accgcttcga aaccgataaa tgggacgtta tggatgccga tgttcgcttc aaagggcggc
    44101 gcattgagca tggcagtagc ctgccgatta gcgatctttc tactcatatc atcctcaaaa
    44161 atgctgacct gcgcctgcaa ccgctgaaat ttggcatggc gggcggcagc attgcggcga
    44221 atattcatct ggaaggcgat aaaaagccga tgcaggggcg ggcagatatt caggctcgtc
    44281 gactgaaact gaaagaactg atgcccgatg tggaactgat gcagaagacg ctgggggaaa
    44341 tgaacggtga cgcggaacta cgcggtagcg gtaactcggt ggcggcgctt ttaggcaaca
    44401 gtaacggcaa cctgaaactg ttgatgaatg acgggctggt gagccgcaac ctgatggaga
    44461 ttgttgggct gaatgtcggc aactacattg tcggtgcgat atttggtgac gatgaggtgc
    44521 gggtgaactg cgcggcggcg aatctgaata ttgccaacgg cgtggcgcgc ccgcagattt
    44581 ttgctttcga tactgagaac gcgttgatta atgttaccgg cacggcaagt tttgcttcgg
    44641 aacagctgga tttgactatt gatccggaga gtaaagggat tcggattatc acactgcgtt
    44701 cgccgctgta tgtgcgtggg acgtttaaaa atccgcaggc tggggtgaaa gccggaccgc
    44761 tgattgcccg tggtgctgtt gctgcggcac tggcaacgct ggtaacaccg gcggcggcgt
    44821 tactggcact gatctcacct tccgaagggg aggctaatca gtgtcggacg atattgtcgc
    44881 agatgaagaa gtgatgataa ttgccggatg tctgacatcc ggcaattaaa cagatttaga
    44941 tttttggcaa agtgtgcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
    45001 aaatccttgt gaattcaata tattgtatgg aaattcagtc ctgataagcg tagcgcatca
    45061 ggcttttcac acttattaca acgactgatg tcgcgtctca tgggtcagca gcagggcgat
    45121 taacgtcaag ccagccatcg ccgccagata taaccccacc gcacctaacc cgtagttagc
    45181 ctgcaaccag gctgcgatat acggcgcaac ggaagccccg agaatcgacg atacgttgta
    45241 agagaacgat gctccggtgt aacgcacttc tgtcggaaac agctctggta acagcgcacc
    45301 catcggcccg aaggtcagcc ccatcagact taaccccagc agcaggaagg caaaaaccag
    45361 aatcgggttg ccagaaccga gcagcgggtt aaaggcgaac agcgcgaaca ggatgatcag
    45421 cgtggtgatg atcaccatgc ttttacggcg accaaaggca tcagccagta atccggcgac
    45481 tggcaccatc acgccaaaac caataactgc catcatcagc atccacaaca cttcgttacg
    45541 cggcaggcca agcccaactg gcgcggcggc ggtactgaag gtcatagagt agaccgtcat
    45601 gatgtaaaac agcgtatagg ttgccagcat aatgaacgta cccagtacgg ttacgcgaac
    45661 atgtttggtc agcagcgtac ccagcgggat cttcacctgt tttttcgctt tagcgacttt
    45721 ctcaaacacc ggcgactcat gcagcgacac gcgaacatac aggccgataa tgaccagcac
    45781 cgccgagaag ataaatggca cacgccagcc ccagctcata aactgctcat cggtcagtag
    45841 ccaggaaagc agcaagaaag tgccgttggc aaagaagaag ccaatcggtg cgcctagctg
    45901 tgggaaggag ccatacagtg cacgtttgcg cggtggggcg ttttcagtcg ccagcagcgc
    45961 cgcaccgccc cattcaccgc ctaagcccag accctgacca aatcgagcca gcgccagcag
    46021 cagtggggcg aaaataccaa tcgtggcata gcccggcagc agaccaatca ccacggtcga
    46081 aatccccatc gttagcaacg aggcgaccag cgtcgcttta cgcccaacgc gatcgccaaa
    46141 atgaccaaaa acggcagagc caatggggcg cgcgacgaag gcgatggcga aggtggcgag
    46201 cgactgtagc gttgctgccg ttggatcgcc ctgcgggaag aagatatgcg gaaacacaat
    46261 aacggccgca gtggcgtaaa tgtaaaagtc gaagaactca atggccgtgc caatgagaga
    46321 ggcgacaagg actttattac gcgagttgat cggcgtgtat tcttgttcgt ggtcgagtgt
    46381 tgtggctgtt gcttgcataa tcttttctta ttttttggct aacgaatagc catattacgc
    46441 acagcaaaag tgatatttca atgtgtaaca aatcgcggaa atggtcaaaa accaggcaaa
    46501 aaggggataa tgtttaggcc agtgattttt cttctatgaa atgcttcaca taattttata
    46561 aatagtggat aaaactggtt tcaggttaaa taaaagttac caactggatt tatatgctgg
    46621 ggttatgagt tggagtgaaa ttgtttgctc ccaaatagat ggctgaagag gctctgggga
    46681 agagtttcaa gaggttatgt gcgcattata gccggatgcg atgctggcgc atcttatccg
    46741 gcctacgggg agccaggccg aataaaatcg tatgccggat aaggcgttta cgccgcatcc
    46801 ggcctatttt aaggctgcgt tttccctggc attcgcgggt cgtctttata ttcggcggtg
    46861 gcaatccacg ccgcgcaaaa cagcgtcaaa cgggcgaaga agtagaaaaa tgccatcagc
    46921 cccagcacgg aaccaaatgc tgcgccagag ggggatttca tcagcgatgg cagggtgtag
    46981 gtcatcacga ttttaatcac ttcaaaacca atagcagcca gaaatgttcc gcgaatcagg
    47041 gcttttttgc gcggacggtg gcgcggcaac cgccagaaga tccagaagaa aagcagatag
    47101 ttggcgaaga tggaaatcgc caggccaatc aatcgccacg tcggtttcag ccactcaatg
    47161 ctgttcaggt gcagggcgct aataatcatt tgctgcgccg aaccggcaac cgaggtgatc
    47221 gaaagcgtca caatcagcgc aatcaacaaa ccaatcagtg aaataaaatc acgcagatat
    47281 tttacccaga acttctcctg atcttgcggc gagcgttccc agacatcgcg cgactgggca
    47341 cgaatcgctt cacgcaggtt acccatccag ttgatgccgg aataaagtgc caccgccagg
    47401 ccgacaagcc ctacagtcgt acgctgctga acggcggtgt tgatggtgtt tttcaacgtg
    47461 gcggctagtg tcggatcgct gatgttttgc agaattttgt cgaagatatc ctgtagcaac
    47521 atcggatggg aggccagcac aaagccccct gcggcaaacg acaccatcaa aatcgggatc
    47581 atcgacaaaa atgagaaata ggtgatagcc gcaccaaact ggttacccag ccgatcgtta
    47641 aagcgttctg tcgcgcgaat aaggtgcgca ataaccggtt ggcgttggac tttttcggca
    47701 gtggtggtga cggtttccag cgcctggcta actttgccgc ctttctcgct attatccaac
    47761 tgcttaatcg gctcgtgctc cagatcctgg gtgggacgtt tgatctcgtt ttcctgcgtc
    47821 atccgctctt ttgtccttct gtcgtggtca ttcttaaaaa gtatagtcgg tcagtccagg
    47881 tgctctttca ttacgccagc cagccactgc ataaacaggt ttacacgccg ggaaagctcc
    47941 cgacgctgcg ggtaaaccag cgaaagggag agcggctcgg cacggtagcc tggcaacact
    48001 tcaataagcc gcccggcacg cagggcttcg cgcacggcaa tgcgcgggat ctgaataatc
    48061 cccagaccgg caagaccggc ggcgagatag ttttcgctac tgtttaccgt caacatgccg
    48121 ccagacttaa accactggac gccattaacg ctggcaacct caaaacctaa cggatgtaca
    48181 cccaggtgcg gtgtataacg cactattgcg tgtgaagtca gatcatcggg gctttgcgga
    48241 taaccaaagc gcgtcagata gtgcggactg gcacagttga ccatggttag tttgccgagg
    48301 ggacgggcga taacgccatc ttccggtaac gcgccagtgc gtatcacgca atcaaaacca
    48361 tcatgaagaa tatctaccgg acggttatgg ctactcagtt ccagctcaat tcccggatgc
    48421 tgatagagaa attccgacag acgcggcagt aacaggtttt tcgcgattcc tgacgggatg
    48481 tcgatgcgta atttaccgct gatactggtg gcatcctgtt gaaacagacc gtccagttcg
    48541 ctgagattac tcaacacatc ttttgcccgt tgataatagg tcatgccttc tggcgtgagt
    48601 ttgacccgtc gcgtagtgcg ctggagaagc tgggtgccaa gttgatgttc cagtgcctgt
    48661 atctggcgcg aaacacttcc ctttggcaaa gcaaagaaat ccgccgcgcg ggaaaaactt
    48721 tccagctccg cgactttgat gaacaactgc attgcgtgaa ttttatccat ttctttgacc
    48781 gattgttgtt cacagcgaaa cagtaacgcg catatcgcat tgtttattgt tttttctaac
    48841 atctaatgcc gggtttcaca atgttgtcat gacgaatgtg gcgatttctc agcaggaaaa
    48901 gggcggaata gctttatgag aaaggtgaat ccccttaaac agagcgtatt tttcagtgat
    48961 tccctttaaa gtgaatagag gacgtattgg ctatttgtgc ttatttttgt ctcattgcca
    49021 tttgttattt tggcttgtag tcgcggaatt tcatttttaa ttgcatgcgt actaaccacc
    49081 aatataagga aatcctcaaa attgttttaa atagtgccct cgacatgtaa tttcaggcgt
    49141 ttagctgtaa ctatttatat cggtaactat tttctacgcg tcatctctgt agaatggcca
    49201 ccgccaaatg gcgaattctt attttttctc gcgggtaata cccttccact acttaagtct
    49261 ttttaacgta ttcaggtgga gattcagtta tgcaaatagt catgtttgac aggcagtcaa
    49321 tatttattca tggaataaaa atcagtttac agcagcgtat tccaggagtg agtattcagg
    49381 gggtcagtca ggcagacgag ttatggcaaa agctggaaag ttaccctgaa gccttagtta
    49441 tgctcgatgg cgatcaggat ggtgagtttt gctactggtt gctgcaaaaa accgtggtgc
    49501 agtttcctga ggttaaggtg ttaatcacgg cgacggattg caataaacgg tggttacagg
    49561 aagttatcca ttttaatgtg ctggccattg tgcctcgcga ttcaaccgtc gagacatttg
    49621 cgttggcggt aaatagtgcg gcgatgggga tgatgtttct gcctggagac tggcggacga
    49681 cgccggaaaa ggacattaaa gatctcaaat cgttaagcgc ccgccagcgg gagattttaa
    49741 ccatgttagc ggcgggcgaa tcaaataagg agatcggcag agcgctgaat atcagtaccg
    49801 gaacggtaaa agcgcatctg gaatccctgt atcgccgtct ggaagtgaaa aatcgcaccc
    49861 aggcggcaat gatgttaaat atctcctcct g
//
LOCUS       DAECNO010000016        49425 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.016, whole genome shotgun sequence.
ACCESSION   DAECNO010000016 DAECNO010000000
VERSION     DAECNO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.016"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1156)
                     /gene="setB"
                     /locus_tag="KKO08_000955"
     CDS             complement(<1..1156)
                     /gene="setB"
                     /locus_tag="KKO08_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBD6317301.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1524..2654
                     /gene="fruB"
                     /locus_tag="KKO08_000956"
     CDS             1524..2654
                     /gene="fruB"
                     /locus_tag="KKO08_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBD6317302.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2654..3592
                     /gene="fruK"
                     /locus_tag="KKO08_000957"
     CDS             2654..3592
                     /gene="fruK"
                     /locus_tag="KKO08_000957"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBD6317303.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3609..5300
                     /gene="fruA"
                     /locus_tag="KKO08_000958"
     CDS             3609..5300
                     /gene="fruA"
                     /locus_tag="KKO08_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBD6317304.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5724..6665
                     /locus_tag="KKO08_000959"
     CDS             5724..6665
                     /locus_tag="KKO08_000959"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBD6317305.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6653..7591
                     /gene="psuG"
                     /locus_tag="KKO08_000960"
     CDS             6653..7591
                     /gene="psuG"
                     /locus_tag="KKO08_000960"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBD6317306.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7685..8935
                     /locus_tag="KKO08_000961"
     CDS             7685..8935
                     /locus_tag="KKO08_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD6317307.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="KKO08_000962"
                     /pseudo
     CDS             complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="KKO08_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="KKO08_000963"
                     /pseudo
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="KKO08_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10872..12122
                     /locus_tag="KKO08_000964"
     CDS             10872..12122
                     /locus_tag="KKO08_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBD6317308.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13266)
                     /locus_tag="KKO08_000965"
     CDS             complement(12178..13266)
                     /locus_tag="KKO08_000965"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBD6317309.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="KKO08_000966"
     CDS             complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="KKO08_000966"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBD6317310.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14200..15249)
                     /locus_tag="KKO08_000967"
     CDS             complement(14200..15249)
                     /locus_tag="KKO08_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBD6317311.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15348..16229
                     /locus_tag="KKO08_000968"
     CDS             15348..16229
                     /locus_tag="KKO08_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6317312.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16435..17904
                     /gene="lysP"
                     /locus_tag="KKO08_000969"
     CDS             16435..17904
                     /gene="lysP"
                     /locus_tag="KKO08_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBD6317313.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18196..20187
                     /gene="cirA"
                     /locus_tag="KKO08_000970"
     CDS             18196..20187
                     /gene="cirA"
                     /locus_tag="KKO08_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBD6317314.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="KKO08_000971"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="KKO08_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="KKO08_000972"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="KKO08_000972"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBD6317315.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23073
                     /locus_tag="KKO08_000973"
     CDS             21997..23073
                     /locus_tag="KKO08_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBD6317316.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23215..24255
                     /gene="galS"
                     /locus_tag="KKO08_000974"
     CDS             23215..24255
                     /gene="galS"
                     /locus_tag="KKO08_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBD6317317.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24535..25533
                     /gene="mglB"
                     /locus_tag="KKO08_000975"
     CDS             24535..25533
                     /gene="mglB"
                     /locus_tag="KKO08_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBD6317318.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25594..27114
                     /gene="mglA"
                     /locus_tag="KKO08_000976"
     CDS             25594..27114
                     /gene="mglA"
                     /locus_tag="KKO08_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBD6317319.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27130..28140
                     /gene="mglC"
                     /locus_tag="KKO08_000977"
     CDS             27130..28140
                     /gene="mglC"
                     /locus_tag="KKO08_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBD6317320.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28176..29389)
                     /gene="preA"
                     /locus_tag="KKO08_000978"
                     /pseudo
     CDS             complement(28176..29389)
                     /gene="preA"
                     /locus_tag="KKO08_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29383..30621)
                     /gene="preT"
                     /locus_tag="KKO08_000979"
     CDS             complement(29383..30621)
                     /gene="preT"
                     /locus_tag="KKO08_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBD6317321.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30815..31054)
                     /locus_tag="KKO08_000980"
     CDS             complement(30815..31054)
                     /locus_tag="KKO08_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBD6317322.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="KKO08_000981"
     CDS             complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="KKO08_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBD6317323.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="KKO08_000982"
     CDS             complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="KKO08_000982"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBD6317324.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32940..33635)
                     /locus_tag="KKO08_000983"
     CDS             complement(32940..33635)
                     /locus_tag="KKO08_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBD6317325.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33632..34030)
                     /locus_tag="KKO08_000984"
     CDS             complement(33632..34030)
                     /locus_tag="KKO08_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBD6317326.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34269..35216
                     /gene="dusC"
                     /locus_tag="KKO08_000985"
     CDS             34269..35216
                     /gene="dusC"
                     /locus_tag="KKO08_000985"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBD6317327.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="KKO08_000986"
     CDS             complement(35587..35670)
                     /gene="yohP"
                     /locus_tag="KKO08_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBD6317328.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35894..37330
                     /gene="mdtQ"
                     /locus_tag="KKO08_000987"
     CDS             35894..37330
                     /gene="mdtQ"
                     /locus_tag="KKO08_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBD6317329.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            37383..38144
                     /locus_tag="KKO08_000988"
                     /pseudo
     CDS             37383..38144
                     /locus_tag="KKO08_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38261..38839)
                     /locus_tag="KKO08_000989"
     CDS             complement(38261..38839)
                     /locus_tag="KKO08_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6317330.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39009..39596
                     /locus_tag="KKO08_000990"
     CDS             39009..39596
                     /locus_tag="KKO08_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBD6317331.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39770..40702
                     /gene="pbpG"
                     /locus_tag="KKO08_000991"
     CDS             39770..40702
                     /gene="pbpG"
                     /locus_tag="KKO08_000991"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBD6317332.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40740..42455)
                     /gene="dld"
                     /locus_tag="KKO08_000992"
     CDS             complement(40740..42455)
                     /gene="dld"
                     /locus_tag="KKO08_000992"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD6317333.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42651..44948
                     /gene="bglX"
                     /locus_tag="KKO08_000993"
     CDS             42651..44948
                     /gene="bglX"
                     /locus_tag="KKO08_000993"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBD6317334.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45200..46006
                     /locus_tag="KKO08_000994"
     CDS             45200..46006
                     /locus_tag="KKO08_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD6317335.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEFWLTPAPANNTWTIAVRQDVA
                     EKNKLTSLADLSRYLQEGGTFKLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGG
                     DTAVTIKAAAQQTSGVNAAMAYGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLK
                     EYPQMAQWLQPVFASLDAKTLQQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46013..47170
                     /gene="yehY"
                     /locus_tag="KKO08_000995"
     CDS             46013..47170
                     /gene="yehY"
                     /locus_tag="KKO08_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBD6317336.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47163..48089
                     /gene="yehX"
                     /locus_tag="KKO08_000996"
     CDS             47163..48089
                     /gene="yehX"
                     /locus_tag="KKO08_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBD6317337.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48094..48825
                     /gene="yehW"
                     /locus_tag="KKO08_000997"
     CDS             48094..48825
                     /gene="yehW"
                     /locus_tag="KKO08_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBD6317338.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48806..48913)
                     /locus_tag="KKO08_000998"
     CDS             complement(48806..48913)
                     /locus_tag="KKO08_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBD6317339.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(48973..49425)
                     /locus_tag="KKO08_000999"
                     /pseudo
     CDS             complement(48973..49425)
                     /locus_tag="KKO08_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11920 a        12179 c        13280 g        12046 t
ORIGIN      
        1 aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga
       61 tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc gacgtgttgg
      121 tatagagcgt ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc
      181 cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg
      241 caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc
      301 gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga
      361 tttccagccc ggcggcggtc cccatcatca caccggctag tttctcggga agatgcagtt
      421 cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg
      481 tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc
      541 cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc
      601 aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta
      661 acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag
      721 agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca
      781 tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat agcaaaacaa
      841 agtagttgcg attccaagca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga
      901 caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac tgacttacca
      961 gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta
     1021 cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca ataccggtga
     1081 gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg
     1141 cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct
     1201 tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa atgccatttc
     1261 actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca
     1321 tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt
     1381 tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg
     1441 atcttgtctt taaccctaag ctaggttggc gctttttttc tcatagaggc tgaatcgttt
     1501 caattcagca agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg
     1561 aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg
     1621 gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt
     1681 tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat caggtgctga
     1741 aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag
     1801 cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg ctacgccagc
     1861 tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag
     1921 aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg
     1981 aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg
     2041 cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac
     2101 aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta
     2161 gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca
     2221 tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag cgtctcgctg
     2281 atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg
     2341 cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtagtgc
     2401 gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat
     2461 ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca aacggacgta
     2521 gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc
     2581 agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct gctggtcttg
     2641 gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg cttatgacct
     2701 tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct
     2761 gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt
     2821 caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga
     2881 gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct
     2941 gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggtttcgaag tcacccccgc
     3001 cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg
     3061 tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg
     3121 cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg
     3181 tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg
     3241 ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg
     3301 tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg
     3361 cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc
     3421 tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg
     3481 tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg
     3541 tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga
     3601 gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata
     3661 tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc
     3721 cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga
     3781 acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc
     3841 tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg
     3901 ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac
     3961 acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga
     4021 aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg
     4081 cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta
     4141 aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag gaactggata
     4201 aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg
     4261 aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc
     4321 tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag
     4381 cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct
     4441 ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc gatcgtccgg
     4501 gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg
     4561 gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaactga
     4621 aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc
     4681 tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag
     4741 ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc
     4801 tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg
     4861 gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta
     4921 tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag
     4981 cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg
     5041 caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg
     5101 cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg cacggtggtc
     5161 tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg gtagcaatta
     5221 ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg
     5281 cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtaa
     5341 catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga atttaaatgg
     5401 ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt tcctcaaggg
     5461 cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca caaattcctc
     5521 gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg
     5581 cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata
     5641 tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt
     5701 aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg
     5761 cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag
     5821 gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc
     5881 tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt caatcgctgc
     5941 taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa
     6001 atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt gctataaatg
     6061 acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc
     6121 agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg gcatggattc
     6181 tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgcgg aaatgtgtca
     6241 aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt gaagcggaaa
     6301 ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc
     6361 atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg
     6421 atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg
     6481 gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg
     6541 ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca
     6601 ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacaca gaatgtctga
     6661 attaaaaatc tcccctgaat tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa
     6721 caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca
     6781 aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc
     6841 cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt
     6901 actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt
     6961 tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc
     7021 cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac
     7081 cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc
     7141 cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt
     7201 gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt
     7261 tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg
     7321 gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat
     7381 gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg
     7441 cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccggcgg
     7501 tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat
     7561 tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt
     7621 actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat
     7681 cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct
     7741 gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca
     7801 aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag tagaacaggc
     7861 ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg
     7921 ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta tcttcgcctt
     7981 tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg
     8041 cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc tcaacatcag
     8101 caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg agatcccggc
     8161 gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag
     8221 cgggatggcg tccattgctg gttcggtgat gattggttat gccggaatgg gcgtaccaat
     8281 tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt ttgcacgtat
     8341 tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac
     8401 gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc
     8461 cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat
     8521 tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg
     8581 ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaaccttgc
     8641 tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc
     8701 cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta tatcctttgc
     8761 gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat
     8821 ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg cagcagcaac
     8881 gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg cgtaatgctc
     8941 gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat
     9001 aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag cgccagtcct
     9061 gacaactgct ttctgttttt aattagatat cctttctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcggca aagaaatcga
     9481 tcagcgacac gcgaccattg gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcattggcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ctaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt
    12481 cactgcggca ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc
    12541 cggtaaagtg ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt
    12601 gaattctgaa acggtatcga caaacaccgg gatctcatca gcgagggtaa agacccattc
    12661 cagcgcctcg gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc
    12721 gcgcgacccg ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaattgc
    12781 cagcacggtt tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg
    12841 caaacgaacg cagccggaga cattcacgcc ggcacggcgc gtttcttcca ggagcatttc
    12901 gccataaaag tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag
    12961 attgtgggcg atattgcgtc caacgccgcc tgccgaacaa tgaattgtac cgggatgaga
    13021 agccgcttgc gggtaatgga tatccgccat cccgcgaata tccatattga ttgtccccac
    13081 cactacgcag tattcctgct cggtgagaat gtaacctttg tctttaatcc ggcctttgcg
    13141 cattaaatcc ataatatgcg ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat
    13201 ttcgttctgc tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt
    13261 attcatcttc aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    13321 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc
    13381 tgcataatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    13441 tcaacgcggc tgccaaaggt acttttcgca tcgttaaggt gcatcccgcg cagatactta
    13501 aagccgacaa tacgggcaaa atcagcgaac gttttttcgc attcggctgg agtatgcaaa
    13561 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct
    13621 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    13681 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    13741 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    13801 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    13861 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttgatcag ataactgtcg
    13921 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg
    13981 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    14041 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    14101 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    14161 ggtaatgata gcgggttaac aggagcaaga agtagtggtt tatgcgatga cgctttgaat
    14221 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    14281 cagaggtttc gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    14341 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    14401 taacaggtgg aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat
    14461 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    14521 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    14581 ttctgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    14641 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    14701 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    14761 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    14821 gatactgcta ccggcaccga tcaaccaact ggtgtgctta tccagaccaa acactttctg
    14881 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    14941 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    15001 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    15061 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    15121 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    15181 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    15241 attcgtcata accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata
    15301 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    15361 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    15421 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gactgacctg gaagggcagc
    15481 ttggcgttca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    15541 tgctctatcc gcgagcgttg gcactgcttg aacaggcggt tgaaatcgaa caactgtttc
    15601 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    15661 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    15721 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg
    15781 aaggaccgtg ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagcaggtgg
    15841 ttttcgccgc gccgacttcg ccgttggctc gtggtccggt caccttagaa cagctggccg
    15901 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggatattgtc gattatctgt
    15961 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    16021 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc
    16081 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    16141 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    16201 attgcgatcc cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    16261 cgatttcgtg acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac
    16321 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    16381 gccaatcgtc tgctacaatc gcgccatcat ttttaagatg gatagcattt ttgtatgggt
    16441 tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg
    16501 acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg
    16561 atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt
    16621 tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc cggttcgttt
    16681 gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac
    16741 tggtacaact gggcggtgac tattgccgtt gacctggttg cagcacagct ggtcatgagc
    16801 tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc
    16861 ctgttgaact acatctctgt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc
    16921 aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc
    16981 aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt
    17041 ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag
    17101 ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta
    17161 cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg
    17221 attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt
    17281 ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca
    17341 gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc tactcgtatg
    17401 ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt
    17461 ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg cttcctgacc
    17521 tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt
    17581 tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg
    17641 cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc
    17701 ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa
    17761 gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt
    17821 atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag
    17881 ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag
    17941 agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt aatatttgga
    18001 ttgataattg ttatcgtttg cattatcgtt acgacacaat caaaaaaggc tgacaaatca
    18061 gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg
    18121 ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc
    18181 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    18241 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    18301 actgcatctt ccgtggaaca aaatcttaaa gatgcacccg ccagtatcag cgtcattacc
    18361 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    18421 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    18481 agcagctaca ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    18541 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    18601 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    18661 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    18721 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    18781 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    18841 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    18901 gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac
    18961 ggtttcgacc gtcaggatcg tgattccgat tcgctggaca aaaaccgcct ggaacgccag
    19021 aactactgcg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatcctac
    19081 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    19141 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    19201 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    19261 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    19321 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    19381 ccgcgtgcct acatggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    19441 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    19501 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    19561 gagctggggc tttactacat gggtgaagaa ggctggctgg aagggggtga atccagcgtt
    19621 atcgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac
    19681 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    19741 ccggtattta gctactacag cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    19801 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    19861 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    19921 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    19981 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    20041 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    20101 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    20161 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    20221 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    20281 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    20341 cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    20401 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactat
    20461 cccattccag catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac tctggatcca gtcgcggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    22381 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    22441 cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    22501 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    22561 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    22621 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    22681 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    22741 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt
    22801 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    22861 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    22921 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    22981 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    23041 gccggaccgg caatatctaa aacatcaaga taacgatctg gattacattc gttaacaaaa
    23101 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    23161 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    23221 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    23281 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    23341 ctggattatc ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    23401 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    23461 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    23521 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    23581 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    23641 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    23701 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    23761 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    23821 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    23881 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    23941 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    24001 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    24061 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg
    24121 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    24181 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    24241 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    24301 tagctatgat tgcaccgttt taatgttgta acccgtatgt aacagtgaat aatcactttc
    24361 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    24421 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    24481 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    24541 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    24601 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    24661 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    24721 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    24781 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    24841 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    24901 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    24961 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    25021 cagattcagt tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    25081 acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat
    25141 accgcaatgt gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    25201 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    25261 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    25321 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    25381 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    25441 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    25501 aaagacaact tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    25561 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    25621 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    25681 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    25741 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    25801 attttattcc agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt
    25861 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    25921 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    25981 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    26041 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    26101 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    26161 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    26221 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    26281 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    26341 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    26401 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    26461 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    26521 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    26581 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    26641 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    26701 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    26761 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    26821 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    26881 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    26941 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    27001 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    27061 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    27121 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    27181 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    27241 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    27301 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    27361 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    27421 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    27481 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    27541 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    27601 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    27661 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    27721 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    27781 gaagcggcaa aaatatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    27841 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    27901 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    27961 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    28021 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    28081 ggggcgatta ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaagtga
    28141 tgtgattgcg gatattgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    28201 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    28261 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    28321 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    28381 catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    28441 acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg
    28501 agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta ctgcataatg
    28561 ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc atcttcccag
    28621 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    28681 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    28741 gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    28801 ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    28861 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    28921 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    28981 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagctgct
    29041 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    29101 ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca atctgctcca
    29161 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    29221 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    29281 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    29341 tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    29401 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    29461 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc gggtctgata
    29521 attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat ccagtctggc
    29581 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ctgataaccg
    29641 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    29701 cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa actcatctaa
    29761 ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    29821 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    29881 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa
    29941 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttccgc
    30001 tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc
    30061 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    30121 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    30181 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    30241 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    30301 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    30361 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    30421 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    30481 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    30541 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    30601 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc
    30661 taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta agctgtgaaa
    30721 ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaata
    30781 attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata agataaacaa
    30841 tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg aagaagagtc
    30901 cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa
    30961 tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa
    31021 aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa
    31081 ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg gcacctggtg
    31141 catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag
    31201 gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt
    31261 cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc
    31321 tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg
    31381 tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg
    31441 gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat aactttgcaa
    31501 tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc
    31561 tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat attttgctgt
    31621 gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg
    31681 cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat
    31741 caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag ctaaggaaaa
    31801 tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg
    31861 aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac
    31921 caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg
    31981 gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac cgcgcgctgg
    32041 atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg caacggcggc
    32101 agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta
    32161 cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct
    32221 gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc
    32281 atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc
    32341 agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct gttcagttca
    32401 ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc tgcaagcgct
    32461 ttttcaccac tcaaccaagc gtggctgatt gcgctttgtt cggcatgaac ggtttgctgc
    32521 attgttgcac caataaattc catattggca ccaaaatacc aggttccgct cacaccgcgc
    32581 gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga
    32641 agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag cgatgagact
    32701 tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat
    32761 tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct
    32821 cataatcatg taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa
    32881 tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt
    32941 tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc
    33001 ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag
    33061 cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta
    33121 cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa
    33181 atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca
    33241 ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc
    33301 aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg
    33361 atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc
    33421 gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtaactgtcg
    33481 taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg
    33541 ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac
    33601 gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga
    33661 ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa accaccagcg
    33721 tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa tcaaaatatt
    33781 gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc
    33841 accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca aacaggatct
    33901 gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg
    33961 catacaggca ggcataaatc aggatgaaag cgcgtaaata ttgccagata atgttcagtg
    34021 tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac
    34081 gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt attacgcaat
    34141 gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct
    34201 ctttgcagac ctttaatccg ctgaccatag gcgctaacat agcgcctcat ttttttgcgg
    34261 gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct caggtgcgtg
    34321 aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg
    34381 atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa aacgccagcc
    34441 ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca caatggctgg
    34501 cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc aattgcggct
    34561 gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa gatcctgaac
    34621 tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg
    34681 tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc
    34741 aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag ggttaccgcg
    34801 cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga
    34861 ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg atcagcgact
    34921 gcgacgcagt gatgattggt cgcggggcgt tcaatattcc caacctgagc cgggtggtaa
    34981 aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc
    35041 gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga
    35101 gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga
    35161 ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa ctctaaaaac
    35221 catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
    35281 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg atgtgggctg
    35341 agggcgcgtt ttacagggat gttacctcgc gcccgacccg gtagccgtaa gggataagcc
    35401 gagggcaccg cgcagcggcg attttgttcg ccagagcccg ggggggcagg gggcggcggc
    35461 gattggtcgc cccctgcgcg ctccttgcgc cagtgattat atgaagcaat gcacatgaca
    35521 ggagcgcaac aggatgagag actgaaatat tctcgttatt ttcggatctg atgacattaa
    35581 ttaattttaa aatatcatct taaatacgcc agtcaccacc agtagcccaa tcaggaaaat
    35641 aatcaataca gcccagagta taattttcat tggcgttcct ttatttgtga gtgctgaata
    35701 agtataggaa aaaacgaagt cctttgccgt tatccataaa cgattaagat aaatcggaag
    35761 atatcattta cttagtctat atttttctgg gtatgtcgtt ttattgctac tgatgtattg
    35821 ccacgcgaaa ctaaaaaata tatcgcaaca cacggtaatc tccgcgcacc gtttcttacc
    35881 tgattattcc cccatgaatc gtgattcctt ttatccagcc atcgcctgtt ttccgctgtt
    35941 actgatgctg gccgggtgtg cgcctatgca tgaaacccgc caggcgttaa gccagcaaac
    36001 gcccgctgca caagttgaca ccgcattacc cacggcgctg aaaaatggtt ggccagacag
    36061 ccaatggtgg ctggagtatc acgataatca gctcacttca ttaattaaca atgcactaca
    36121 gaacgcaccg gatatgcagg tggctgaaca gcgcattcag ctagctgaag cccaggcgaa
    36181 ggcagttgcc actcaggatg gtccgcagat cgacttttcg gcggatatgg agcggcaaaa
    36241 aatgtcggca gaagggctaa tggggccgtt tgctctgaac gatccggccg caggtacgac
    36301 cggtccgtgg tacaccaacg gaacttttgg cttaacggcg ggctggcatc tcgatatctg
    36361 gggaaagaat cgggcggagg ttactgcccg cctgggtacg gttaaagcac gggcggcgga
    36421 acgcgagcaa acccgccaat tgctggctgg cagcgtagcc cgcctgtact gggagtggca
    36481 aacccaggcg gcgttaaaca cggtcttgca gcaaatagaa aaagagcaga acaccattat
    36541 cgcgaccgat cgccagctat atcagaacgg gattacttct tcagttgacg gtgtggaaac
    36601 cgatattaat gccagcaaaa cccggcagca gctcaacgat gtcgcgggga aaatgaaaat
    36661 tattgaggca cggttaaatg cactcacaaa tcaccagact aagtcattga agcttaaacc
    36721 ggtcgcgttg ccgaaagtgg caagccagct tcctgatgaa ctggggtact ccttactggc
    36781 ccggcgggca gatttgcagg cggcgcactg gtacgttgag tcatcgttaa gcactattga
    36841 tgcggcaaaa gcggcatttt atcctgacat caacctgatg gccttcctgc aacaggatgc
    36901 gttgcactta agcgatctgt tccgtcattc cgcgcagcaa atgggcgtta cggcaggcct
    36961 gacgctacct attttcgata gtggtcgtct taacgccaat ctcgatatcg caaaagccga
    37021 aagcaacttg tctatcgcca gctacaacaa agcggtggtt gaagcagtga atgacgtggc
    37081 gcgggcagcc agtcaggttc agacactggc ggagaaaaac cagcatcagg cgcaaattga
    37141 gcgcgatgcc ttgcgtgtgg taggtcttgc gcaggcccgc tttaacgcgg gcatcattgc
    37201 tggttcccgc gtcagcgaag ccagaatccc ggcgctgcgt gagcgggcca atggcctgtt
    37261 attgcaaggg cagtggctgg atgcctccat tcgactcacc ggtgcgctgg gcgggggtta
    37321 caaacgctga tgatatgcgc cttctatact taacgtttat tcagcgttaa gtggagaact
    37381 cgatggcata ggtcgcgata attaccgctt ccgattcggg gatcggcaaa gagtgcgcgt
    37441 tattactggc gcagcagggg tttgatattg gcattacctg gcactcagat gaagaagggg
    37501 caaaagatac cgcgcgtgag gtggttaccc acggcgtacg tgcggagatc gtgcaactgg
    37561 atctcggcaa tctgccagaa ggtgcgcagg cgctggagac actcatccag cgatttgggc
    37621 gtattgatgt gctggtgaat aatgcgggtg caatgaccaa agcgccgttt cttgatatgg
    37681 cttttgatga gtggcgcaag atttttaccg ttgatgttga tggtgcattt ttatgctcgc
    37741 aaattgcggc tcgtcagatg gtgaaataag ggcagggcgg tcgcatcatc aacattacgt
    37801 cggtacatga acatacgccg ctgccggatg ccagcgccta cacagccgct aaacatgcgc
    37861 tcggcgggtt aaccaaaacg atggcgctgg agttggtcag gcataagatt ttggtgaacg
    37921 cagtcgcgcc tggggcgata gccacgccaa tgaacagcat ggatgatagc gacgtgaagc
    37981 ccgacgcgga gccttcgatt cctctacggc gttttggcgc aacgcatgag attgccagcc
    38041 tggtggtgtg gctttgttcg gagggcgcaa attacacgac agggcagtcg ttgatagtgg
    38101 atggcggctt tatgttggcg aatccgcagt tcaacccaga atagcgcagt gaggtgcgtg
    38161 gatatttcaa tgggttaggc cagataagac accagcgtcg catcaggcat ggaacacccg
    38221 gtgccggatg tggagtgaat gcttatctgt ggcaactgct ttacgcctga ttatccggct
    38281 ttttcttccc gcgccgtttc agccaccagc gcacgcccac caccagaacc acgaccagaa
    38341 tcaaccagac ccagtgcttc aaatgctggt cgagattgtg caaccacggc gcaatcacct
    38401 gaccaccagc gtaaccaata gtggtaaaaa tcaacgccca ggcaaatgcg ccgagaatat
    38461 tcagcggcag aaagattttc ggcggcagct ggctggcacc aatcagcgtc gggccaatca
    38521 cccgaaagcc atacataaag cgcgtaccaa tgacaaacag atacggatgg cgttggataa
    38581 gtttctgtgc ccgctcaatt ttatcctgat gtttcgagaa acggcgtaac agcttgccgc
    38641 caaaccgccg cccgcacaga tagagcacct ggtcgccaat catgccacca agcgccacag
    38701 aaagtaccac cagtgggaac tttaatagcc cctgatgtgc cgcaacgcct cccagcaaag
    38761 tcacggtttc accttccgcc aggctaccga taaccagcgc ggcataacca tattgtgaga
    38821 tgagtgtatt gagagccata ggccagtttg tctccctttg gttgcctgta agcatacacc
    38881 ctgtgaatca ttcacgctga aaaaggacat acatcagacg gtgcaaatta catggatgaa
    38941 caaaaaataa tcacaagctg gcttatactt gaactgtcag gttcatgaac tcacaacaag
    39001 ggggcggtat gagccatgtc tggggactgt tttcccatcc cgatcgtgaa atgcaggtga
    39061 ttaatcgcga aaacgaaacg atttctcacc attataccca ccacgttttg ctgatggcgg
    39121 cgattccggt gatttgcgcc ttcattggca ctacacagat tggctggaat tttggcgatg
    39181 gcactatcct gaagttatcc tggtttaccg gactggcgct ggctgtcttg ttttacggcg
    39241 ttatgctggc tggggttgcg gtgatggggc gggtcatctg gtggatggcg cgcaattacc
    39301 cgcagcgtcc gtctcttgcg cactgcatgg tcttcgcggg ctacgttgca actccgctgt
    39361 ttttaagcgg tctggtggcg ctttatccac tggtctggtt gtgtgcgctg gtcggcacgg
    39421 tggcgctgtt ttacaccggc tacctgcttt accttggtat tccgagcttc ctgaatatca
    39481 acaaagagga aggccttagc ttctccagtt cgacgctggc gattggcgtg ctggtactgg
    39541 aagtgctgct caccctaacc gttatactat ggggctacgg ttatcgtttg ttctagaaag
    39601 ttctttgacg ttgcattgct ggcgtaaatg tcagcaatgc agcatttact cacgattctc
    39661 ctttgacgat cgcacttttt gctcgttatg atggcgtcgc tagcctcagt aaatccttac
    39721 tccggcggtg cgcaacccgt gcgcgtgaac cactatctga atgctcatca tgccgaaatt
    39781 tcgagtttct ttatttagct tggccctgat gctggctgtg ccttttgcac cgcaggccgt
    39841 tgcgaaaacg gcagccgcta ccaccgcttc acaaccggaa attgcctccg gtagcgcgat
    39901 gattgttgat ctgaatacca acaaagtgat ctattcgaac cacccggatc tggtgcgtcc
    39961 gattgcgtct atcagcaaat taatgaccgc gatggttgtg ctggatgcac gactgccgct
    40021 ggatgaaaaa ctaaaagtgg atatcagcca gacgccggag atgaaagggg tctattcgcg
    40081 cgtacgactg aatagcgaaa tcagccgtaa agatatgctg ttgctggcgc tgatgtcttc
    40141 agaaaaccgc gcggcggcaa gccttgcgca ccattatccc ggtggttaca aagcctttat
    40201 taaggcaatg aatgcgaaag cgaaatcgct cggaatgaac aacacgcgct ttgttgaacc
    40261 taccggattg tcggtgcata acgtttcaac tgcccgtgac ttaaccaaac tgctcattgc
    40321 cagcaaacaa tatccgttga tcgggcagtt aagtactacc cgggaagata tggcaacctt
    40381 ctctaacccg acgtatacgc tgccgttccg caatactaat catctggtgt atcgcgataa
    40441 ctggaatatt cagttaacca aaaccggctt taccaatgcg gcgggccatt gtctggtgat
    40501 gcgtacggtt atcaataata aaccggtggc gctggtagtg atggacgcgt ttggcaaata
    40561 tacccatttt gccgatgcca gccgcctgcg tacctggatt gaaaccggta aagtgatgcc
    40621 tgtgccggca gcagcgttga gctataaaaa acaaaaagcc gcccaaatgg cggcggcggg
    40681 gcagacggca cagaacgatt aagtgaattc ggatggcgat actctgccat ccgtaatttt
    40741 tactccactt cctgccagtt tttccgttta ctggttttac cgatccccgg attcatgcta
    40801 ttggtcggat cattctcgcg atagaacttc tgcaacgtct ccggtgcttt atacaaatga
    40861 ccgacgttat gctcggcagg gtactgcgcg ccgcgctgct gtagcagttc cagcatctgt
    40921 tcttttaacg catgcacatc cacgcctttt ttcactatgt aatcctgatg gaagacatag
    40981 cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc cggcggtaaa
    41041 tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagtgccag aatgtcttcg
    41101 acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc gaaacggtgt
    41161 aaaaacgctt tgctaccttc ctccggcgta cagacaaaga aatcgccctc ggcctgtttg
    41221 aaataatcca ccagccacga tttggcttca ccgacgccat cgcctgccat ttttaacagc
    41281 agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg gctggggaac
    41341 aggtgaccga atttttgcat tgcacggtca gtaaaatgcg gacggaagaa tttcactttc
    41401 tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt gtcggtgcct
    41461 aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc gtagatatcc
    41521 cggtgcatat attccccggc aaccggcaga ttttcgaagt tagccagaat atgacggcgg
    41581 atttcggtca gcacttccgg ctggttggtg ccgatataaa acacctgctg atttttttcc
    41641 gcttcgaagg tatcaagacg taccgcaaag acggccagct tcccggcgca accagaagat
    41701 tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc aatatcacga
    41761 acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac atcgtcatct
    41821 ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc cagatcaatc
    41881 cccagatggt tcaccagcgt cagtttgcca tcttcatcta tacgcgcgaa taacgacatt
    41941 tcggtatacg ccgggccacg ttgcaccagc gagccgccgg agttgttaca aataccgccg
    42001 atgaccgatg cgcctataca cgatgatcca atcaccgaat gcggttcgcg tcccagcggt
    42061 ttgagggctt tttccagcga atagagcgtg gtgcccggat aggccagcac ctgttcgccc
    42121 ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat atcgcgatca
    42181 tagtcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc ctgcatcaga
    42241 ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag tagtgagcca
    42301 gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg atagcgggcc
    42361 gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag ttcattcaaa
    42421 aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg gtggcgaaaa
    42481 aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata ttttgaaaat
    42541 tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca aactacggtt
    42601 tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat atgaaatggc
    42661 tatgttcagt aggaatcgcg gtgagtctgg tcctgcagcc agcactggcg gatgatttat
    42721 tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgat ttgctcaaga
    42781 aaatgacggt tgatgagaaa attggtcagc tgcgcttaat cagcgtaggc ccggataatc
    42841 cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt ttcaacaccg
    42901 taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc cgcctgaaaa
    42961 ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc ccgattagcc
    43021 tcggtctggc ctcgtctttt aacctcgatg cggtgaaaac ggtcgggcgt gtctctgctt
    43081 atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat gtctcgcgcg
    43141 atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc acctcaataa
    43201 tgggtaaaac catggtggaa gcgatgcagg gtaaaagccc ggcagatcgt tactcggtga
    43261 tgaccagcgt caaacacttt gccgcatacg gcgcggtaga aggcggtaaa gagtacaaca
    43321 ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac aaagcggggc
    43381 tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc acgccagcca
    43441 cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt aaaggcatca
    43501 ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg gcagacccgg
    43561 aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc gacgagtact
    43621 actcgaagta tctgcctggg ttgattaaat ccggcaaagt gaccatggca gagctggacg
    43681 atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac gacccataca
    43741 gccatttggg gccgaaagag tctgacccgg tggataccaa tgccgaaagc cgcctgcacc
    43801 gtaaagaagc gcgtgaagtg gcgcgcgaaa gcctggtgtt gctgaaaaac cgtctcgaaa
    43861 cgttaccgct gaaaaaatcg gccaccattg cggtggttgg gccactggcg gacagtaaac
    43921 gtgacgtgat gggcagctgg tccgcagccg gtgttgccga tcaatccgtg accgtactga
    43981 ccgggattaa aaattccgtc ggtgaaaacg gtaaagtgct gtatgccaaa ggggcgaacg
    44041 ttaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg gtcaaagtcg
    44101 atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa caatctgatg
    44161 tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaagcctcc agccggaccg
    44221 atatcactat tccgcaaggc caacgtgact tgattgcggc gctgaaagcc accggtaaac
    44281 cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa gatcagcagg
    44341 ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca attgccgatg
    44401 tattgtttgg cgattacaac ccgtccggca agctgccaat ttccttcccg cgttctgtcg
    44461 ggcagatccc ggtgtactac agccatctga acaccggtcg tccgtataat gccgacaagc
    44521 cgaacaaata cacttcgcgt tattttgatg aagctaacgg agcgctttat ccgttcggct
    44581 atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg ccgaccatga
    44641 agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaag cgcgaggggg
    44701 ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagtcgc cctgtgaaac
    44761 agctgaaagg ctttgagaaa atcaccctga aaccgggcga aactcagact gtcagcttcc
    44821 cgatcgatat tgaggcgctg aagttctgga atcaacagat gaaatatgac gccgagcctg
    44881 gcaagttcaa tgtatttatc ggcactgatt ccgcacgcgt taagaaaggc gagtttgagt
    44941 tgctgtaatg ctttagtaag ggcgcgacgt ttgcgccctt tgcctgatgt tcatatctta
    45001 acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca tccggcagga
    45061 aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga atccttccgc
    45121 tcaggctaac tctgtcatta tcaactatgc ttttctctta attcgctgaa aacaacagta
    45181 aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac tggttatgtt
    45241 ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt caaaaatcga
    45301 taccgaaggc gcgctgctcg gcaacatcat ctttcaggta ctcgaaagcc acggcgtacc
    45361 gacggtgaat aaagtgcaac tcggaacgac tcctgtgggg cgcggggcga ttacttccgg
    45421 tgaactggat atctatccgg aattctggct gacgcccgcg cctgcaaata acacctggac
    45481 tatcgccgtg cgtcaggatg tggcagagaa aaacaaactc acttcgcttg ccgacctgag
    45541 tcgttatctg caagagggcg gcaccttcaa actggcagcc tcggcagagt ttatcgaacg
    45601 cgccgatgcg ttacccacgt ttgaaaaagc ctatggcttt aagctcggtc aggatcagtt
    45661 gctgtcactg gctggtggcg acacggcggt gacgattaaa gccgctgccc agcaaacctc
    45721 tggcgttaat gccgcaatgg cttacggcac tgacggtccg gtcgcggcgc tggggctgca
    45781 aaccttaagc gatccgcaag gcgtgcaacc tatctacgcg cctgcaccag tggtgcgtga
    45841 atcggtgttg aaggagtatc cgcaaatggc acagtggcta cagccagtct tcgccagcct
    45901 cgatgcaaaa acattgcagc aactgaatgc cagcattgca gtggaaggac tggatgccaa
    45961 aaaagtggct gccgactacc tgaaacaaaa agggtgggcg aagtaatttc ccgtgactta
    46021 tctacgtatt aatcctgttc tggcgctgct gttgttgctg acggcaatcg cagcggcgct
    46081 gccgtttatc agttacgcgc ctaatcgttt agtctcgggt gaagggcttc atctctggca
    46141 actgtggccg caaacgatct ggatgctggt gggcgttggt tgcgcctggc tgacagcctg
    46201 ttttgtcccc gctaaaaaag gcagcatttt tgcactcatt ctggcgcaat tcgtcttcgt
    46261 attgttggtg tggggagctg gaaaggcggc gacccaactg gcgcaaaatg gcagtgcgct
    46321 ggcgcgtacc agcctcggca gaggtttctg gctggctgcg gcgctggcat tgctggcctg
    46381 tagcgatgcc atccgccgaa tctccacgca tccgctgtgg cgctggttgt tgcatatgca
    46441 gattgccatt attccgctgt ggttgctgta ctccggcacg cttaacgatc tctcactaat
    46501 gaaagaatac gccaaccgtc aggatgtgtt tgacgacgcg ctggcacaac atctgacgtt
    46561 gctgtttggt gcggtgctgc ctgcgttagt gattggtgtg ccgttgggca tctggtgcta
    46621 cttttccact gcgcggcagg gggcaatttt ttctctgctc aatgtcattc agaccgtgcc
    46681 ttcggtggcg ctctttggcc tgttgattgc gccgcttgcc gcgctggtta cggcctttcc
    46741 gtggctgggg aagctcggca tagcaggaac cggaatgaca cccgcactga ttgcgctggt
    46801 gctctatgcc ttgctgccgc tggtgcgagg cgtggtagtc ggtttgaacc agatcccgcg
    46861 cgatgtgctg gagagcgcca gagcgatggg gatgagcggg gcgcagcgtt tcctgcatgt
    46921 tcagttaccg ctggcgttac tggtatttct gcgcagcctg cgggtggtga tggtgcaaac
    46981 tgtaggtatg gcggtgattg cggcgttaat cggcgcaggc ggttttggtg cgctggtttt
    47041 ccaggggctg ctaagcagcg ccattgattt agtgttgctg ggggtgatcc cggtaattgt
    47101 tctggcggtg cttaccgacg cgctgttcga tttgcttatc gcactgctga aggtgaaacg
    47161 taatgattga atttagccat gtcagcaaac tgttcggcgc acaaaaagcc gttaacgatc
    47221 tcaatctcaa ttttcaggaa gggagttttt cggtgctgat tggcacatct ggctccggca
    47281 aatccaccac cctgaaaatg attaaccgcc tggtggagca tgacagcgga gtgatccgct
    47341 ttgccggaga agaaattcgc tcgctgccag tactggagtt gcgccgccgg atgggctatg
    47401 ccattcaatc tattggcctg ttcccccact ggagcgtggc gcaaaacatc gctaccgtgc
    47461 cgcaattaca aaaatggtcg cgggcgcgga ttgacgatcg tatcgacgaa ttaatggcgc
    47521 tactggggct ggagtcaaat ttgcgtgagc gttatccgca tcagctttcc ggtggtcagc
    47581 agcaacgtgt gggagtggcg cgtgcactgg ctgccgatcc gcaagtctta ctgatggatg
    47641 aaccttttgg cgcactggac ccggtaacgc gcggcgcgtt gcaacaagag atgacgcgca
    47701 ttcaccgttt gctggggcgc actattgtgc tggtcactca tgatattgat gaggcgctac
    47761 ggctggcaga acatctggta ttgatggatc acggtgaagt ggtgcagcag gggaatccgc
    47821 tgacgatgct gactcgtccg gcgaatgatt ttgtccgcca gttttttgga cgtagtgaac
    47881 tgggggtgca cctgctttcg ttacgtagcg tggcggatta cgtgcgtcgc gaagaacggg
    47941 cagaaggtga ggcactggca gaagagatga cgctacgcga tgcgctctcc ctgtttgtcg
    48001 cgcggggatg cgaggtgctg ccggtggtga acacgcaggg ccagccttgc ggcacgctgc
    48061 attttcagga tctgctggtg gaggcgtaag cgtatgaaga tgttgcgcga tccgctgttc
    48121 tggctcattg ctctttttgt ggcgctgatt ttctggctgc cttacagcca gccgctgttt
    48181 gctgccttgt tcccacaact gccacgaccc gtttatcagc aagaaagttt tgcagctctg
    48241 gcactggctc atttctggct ggtgggaatt tcgagtttgt ttgcggtgat cattggcaca
    48301 ggtgccggaa ttgctgttac tcgcccgtgg ggcgcggaat ttcgcccact ggtggaaact
    48361 attgccgccg ttggacagac ttttccgcct gtcgcagtgc tggcgattgc cgttccggtg
    48421 atcggctttg gtctgcaacc agcgattatc gccttgatcc tttacggtgt gctgcccatc
    48481 ctgcaggtga cacttgccgg gctgggagcg attgatccca gcgtgacaga agttgcgaaa
    48541 ggtatgggaa tgagtcgagg tcagcgactg cgtaaggtcg agctaccgct ggcggctccg
    48601 gtgattctgg cgggcgtgcg aacttcggtg attatcaaca ttggtacggc gacgatcgcc
    48661 tcaacggtag gggccagtac gctgggtacg cccatcatca tcgggcttag cggatttaat
    48721 accgcgtatg tgatccaggg ggcgttactg gtggcactgg cggcgatcat cgcagaccgc
    48781 ctgtttgaaa ggctggtgca ggcgcttagc cagcacgcaa aataaaggta taacctgcga
    48841 gcatgacgcc accaattccg cctaacgcca taaacaggaa cagggcgatg accccaattt
    48901 tagctatgcg cataatgcac tccttatgtt aacgaaagga ttgtacagta aagcgcattt
    48961 gttaacgaat cattaaatac cgagtgggaa aatatcatgg ccttgttctt gccaactggt
    49021 gagttgctgc tgttgggcgg aggttcgatt ttcaccgcac cacaccagca atgtacggcc
    49081 ttcgaatagt tcagggcgta gttgattgag cgagtgggcg agcgaggaca tcaatgcgcc
    49141 atccttgttg actggcaatc cagccctcca gccacagacg ggtggtatcc tgaatattcc
    49201 agccaaccac cagcgcatct ttaccctgtt ttttacgtgc cgaagccaga caaatggcga
    49261 tgtagttgat cagtacgccg tcgaggatcg ccagcagcgc ctggagagtc ggttgttggc
    49321 actgaagccg tcggcgcaga ggaataaaca gatgtgtggt gagtgtctgg gcggggtaat
    49381 cctgaccgcg ctctttgatc cacgttcgca ggctatgtag attgc
//
LOCUS       DAECNO010000017        48304 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.017, whole genome shotgun sequence.
ACCESSION   DAECNO010000017 DAECNO010000000
VERSION     DAECNO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.017"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            188..481
                     /locus_tag="KKO08_001000"
     CDS             188..481
                     /locus_tag="KKO08_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD6317340.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(522..1712)
                     /gene="nepI"
                     /locus_tag="KKO08_001001"
     CDS             complement(522..1712)
                     /gene="nepI"
                     /locus_tag="KKO08_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBD6317341.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1923..2375)
                     /locus_tag="KKO08_001002"
     CDS             complement(1923..2375)
                     /locus_tag="KKO08_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBD6317342.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="KKO08_001003"
                     /gene_synonym="yicO"
     CDS             complement(2428..3762)
                     /gene="adeQ"
                     /locus_tag="KKO08_001003"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBD6317343.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3937..5703
                     /gene="adeD"
                     /locus_tag="KKO08_001004"
     CDS             3937..5703
                     /gene="adeD"
                     /locus_tag="KKO08_001004"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBD6317344.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="KKO08_001005"
     CDS             complement(5749..7140)
                     /gene="uhpT"
                     /locus_tag="KKO08_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBD6317345.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="KKO08_001006"
     CDS             complement(7278..8597)
                     /gene="uhpC"
                     /locus_tag="KKO08_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBD6317346.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="KKO08_001007"
     CDS             complement(8607..10109)
                     /gene="uhpB"
                     /locus_tag="KKO08_001007"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBD6317347.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="KKO08_001008"
     CDS             complement(10109..10699)
                     /gene="uhpA"
                     /locus_tag="KKO08_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBD6317348.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="KKO08_001009"
     CDS             complement(10774..11064)
                     /gene="ilvN"
                     /locus_tag="KKO08_001009"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBD6317349.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="KKO08_001010"
     CDS             complement(11068..12756)
                     /gene="ilvB"
                     /locus_tag="KKO08_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBD6317350.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="KKO08_001011"
     CDS             complement(12862..12960)
                     /gene="ivbL"
                     /locus_tag="KKO08_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBD6317351.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13525..13614
                     /gene="tisB"
                     /locus_tag="KKO08_001012"
     CDS             13525..13614
                     /gene="tisB"
                     /locus_tag="KKO08_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBD6317352.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13894..15078
                     /gene="emrD"
                     /locus_tag="KKO08_001013"
     CDS             13894..15078
                     /gene="emrD"
                     /locus_tag="KKO08_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBD6317353.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15086..15583)
                     /locus_tag="KKO08_001014"
     CDS             complement(15086..15583)
                     /locus_tag="KKO08_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBD6317354.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15580..15942)
                     /locus_tag="KKO08_001015"
     CDS             complement(15580..15942)
                     /locus_tag="KKO08_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBD6317355.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15932..16279)
                     /locus_tag="KKO08_001016"
     CDS             complement(15932..16279)
                     /locus_tag="KKO08_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBD6317356.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16388..16801
                     /locus_tag="KKO08_001017"
     CDS             16388..16801
                     /locus_tag="KKO08_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317357.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16848..18290)
                     /locus_tag="KKO08_001018"
                     /pseudo
     CDS             complement(16848..18290)
                     /locus_tag="KKO08_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18325..20040)
                     /locus_tag="KKO08_001019"
     CDS             complement(18325..20040)
                     /locus_tag="KKO08_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBD6317358.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20207..21100
                     /locus_tag="KKO08_001020"
     CDS             20207..21100
                     /locus_tag="KKO08_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBD6317359.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21097..21911)
                     /locus_tag="KKO08_001021"
                     /pseudo
     CDS             complement(21097..21911)
                     /locus_tag="KKO08_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21911..23528)
                     /locus_tag="KKO08_001022"
                     /pseudo
     CDS             complement(21911..23528)
                     /locus_tag="KKO08_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23823..24497
                     /locus_tag="KKO08_001023"
     CDS             23823..24497
                     /locus_tag="KKO08_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD6317360.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24536..26188)
                     /locus_tag="KKO08_001024"
     CDS             complement(24536..26188)
                     /locus_tag="KKO08_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBD6317361.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGKLYWFMPPSANLALRELGIVLFLSVVGLKSGGD
                     FVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPPALA
                     FANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26384..26812)
                     /gene="ibpB"
                     /locus_tag="KKO08_001025"
     CDS             complement(26384..26812)
                     /gene="ibpB"
                     /locus_tag="KKO08_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBD6317362.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26924..27337)
                     /gene="ibpA"
                     /locus_tag="KKO08_001026"
     CDS             complement(26924..27337)
                     /gene="ibpA"
                     /locus_tag="KKO08_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBD6317363.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27331..27403)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27642..27974
                     /locus_tag="KKO08_001027"
     CDS             27642..27974
                     /locus_tag="KKO08_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBD6317364.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27976..29196)
                     /locus_tag="KKO08_001028"
     CDS             complement(27976..29196)
                     /locus_tag="KKO08_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBD6317365.1"
                     /translation="MKQITFAPRNHLLTNTNTNTWTPDSQWLVFDVRPSGASFTGETI
                     ERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVI
                     AEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLR
                     NVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHAL
                     AFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTF
                     THHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTD
                     IQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLA
                     WMEGGQLWITETDR"
     gene            29297..30397
                     /gene="cbrA"
                     /locus_tag="KKO08_001029"
     CDS             29297..30397
                     /gene="cbrA"
                     /locus_tag="KKO08_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBD6317366.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30358..31695)
                     /locus_tag="KKO08_001030"
     CDS             complement(30358..31695)
                     /locus_tag="KKO08_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6317367.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31770..32918)
                     /gene="dgoD"
                     /locus_tag="KKO08_001031"
                     /pseudo
     CDS             complement(31770..32918)
                     /gene="dgoD"
                     /locus_tag="KKO08_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32915..33532)
                     /gene="dgoA"
                     /locus_tag="KKO08_001032"
     CDS             complement(32915..33532)
                     /gene="dgoA"
                     /locus_tag="KKO08_001032"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBD6317368.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33516..34394)
                     /gene="dgoK"
                     /locus_tag="KKO08_001033"
     CDS             complement(33516..34394)
                     /gene="dgoK"
                     /locus_tag="KKO08_001033"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBD6317369.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34391..35080)
                     /gene="dgoR"
                     /locus_tag="KKO08_001034"
     CDS             complement(34391..35080)
                     /gene="dgoR"
                     /locus_tag="KKO08_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBD6317370.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35358..36014
                     /locus_tag="KKO08_001035"
     CDS             35358..36014
                     /locus_tag="KKO08_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317371.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36060..36872)
                     /gene="yidA"
                     /locus_tag="KKO08_001036"
     CDS             complement(36060..36872)
                     /gene="yidA"
                     /locus_tag="KKO08_001036"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBD6317372.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36986..37384)
                     /locus_tag="KKO08_001037"
     CDS             complement(36986..37384)
                     /locus_tag="KKO08_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBD6317373.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37624..40038)
                     /gene="gyrB"
                     /locus_tag="KKO08_001038"
     CDS             complement(37624..40038)
                     /gene="gyrB"
                     /locus_tag="KKO08_001038"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBD6317374.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40067..41140)
                     /gene="recF"
                     /locus_tag="KKO08_001039"
     CDS             complement(40067..41140)
                     /gene="recF"
                     /locus_tag="KKO08_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBD6317375.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41140..42240)
                     /gene="dnaN"
                     /locus_tag="KKO08_001040"
     CDS             complement(41140..42240)
                     /gene="dnaN"
                     /locus_tag="KKO08_001040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBD6317376.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42245..43648)
                     /gene="dnaA"
                     /locus_tag="KKO08_001041"
     CDS             complement(42245..43648)
                     /gene="dnaA"
                     /locus_tag="KKO08_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBD6317377.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43945..44025
                     /locus_tag="KKO08_001042"
     CDS             43945..44025
                     /locus_tag="KKO08_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317378.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44255..44395
                     /gene="rpmH"
                     /locus_tag="KKO08_001043"
     CDS             44255..44395
                     /gene="rpmH"
                     /locus_tag="KKO08_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBD6317379.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44412..44771
                     /gene="rnpA"
                     /locus_tag="KKO08_001044"
     CDS             44412..44771
                     /gene="rnpA"
                     /locus_tag="KKO08_001044"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBD6317380.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44735..44992
                     /gene="yidD"
                     /locus_tag="KKO08_001045"
     CDS             44735..44992
                     /gene="yidD"
                     /locus_tag="KKO08_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBD6317381.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44995..46641
                     /gene="yidC"
                     /locus_tag="KKO08_001046"
     CDS             44995..46641
                     /gene="yidC"
                     /locus_tag="KKO08_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBD6317382.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46747..48111
                     /gene="mnmE"
                     /locus_tag="KKO08_001047"
     CDS             46747..48111
                     /gene="mnmE"
                     /locus_tag="KKO08_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBD6317383.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11992 a        13057 c        12204 g        11051 t
ORIGIN      
        1 agcggtaagg tcgctgcggt ttaaaaaacg aaacgctatc gataagaata aaaaggaata
       61 aaagtggaat ataaagtctg gcacttctta ctgaccacgc aagcgcgttt cgtacagcat
      121 gatgagagcg atgaaagcaa actacactta tgcttcattc gatacacatt tgtaaaaggt
      181 tgaatccatg aaaccaacga cactactgct tattttcact tttttcgcta tgccaggcat
      241 cgtatacgcc gagtcgcctt ttagctcact gcagtccgca aaagagaaaa ccacggtatt
      301 acaagattta cgtaaaattt gcacgccaca tgcgtcatta tcagatgaag cgtgggaaaa
      361 gttaatgttg tcagatgaaa acaataaaca acatatacgc gaggccatcg tggcgatgga
      421 acgtaataat cagagcaatt attgggaagc gttgggtaag gtagaatgcc cggatatgta
      481 agaacagcaa caactactga ccaacgcttc tttacctgat atcaggattt cttcattttc
      541 acctttgcag taaccaacaa tgcagtcagc aacatcaatg tgccggacaa catcaacggc
      601 gaagtcagac caatattatc cagcgcataa ccgccgattg ccgcgccaca ggtattagca
      661 agctgaataa ccgccacctg aatagacccg gctttttctg cctgatcggc cagcgagcgg
      721 gtgatccacg ttgaccagcc gacgggaacc aatgcaaaag ttagccccca gataatcgcc
      781 acgccggtag caacgatttt atcgcttccc cacaacgtca gtaccaacgc actcacagcc
      841 agtattaacg gcgcgcctgc taaggccagt tttaccgaac gtttaagaat gaacgacgaa
      901 agcgacgtac caataaagct ggcgatacca aaactcaaca gcaccagcgt taagccatcc
      961 acgccgaatc ccgccaggtt catatacacc gggcgaatat acgtgaagaa agcaaactgc
     1021 ccggcgaaag acatgaagat ggcgatcatc cctgccatca cacccggacg ttgtaataag
     1081 cggaaagtat tttgtttctg atgcgagggt tcgcctggca gtgaaggcaa tgatttgata
     1141 atccagaaaa tacacagcac gcccatcacc gccgccgcat taaagacatt gcgccaaccg
     1201 ataagctcgc ctaaaaaact gcccaacggc gcggcaatca ccagcgcaat agaaaccgcg
     1261 ccgaagatca ccgacagcgc cttcggcacc gtacgcggcg gtaccagacg catggtcagc
     1321 gacgccgaca tcgcccagaa cccgcccagc gccagcccca gacaggcacg accgattaaa
     1381 agcaaactga atgagttagc aaaggaaacc agcaagcagg agagcgtcag caaaacggca
     1441 aacaaaataa caacgtagcg gcggtcagtg gcctgaattg tctgggtaat aaacaaactg
     1501 gcaaacattg ccacaaaggc ggtcacggtc accgattgcc cggcaacccc ttccgaaatg
     1561 cctaaatcct gggccattgg cgtcaacaaa ctgacgggca aaaactcaac gataatcaga
     1621 caggcgacac aaaacgccac cgagaaaacg gctgaccagt tcggtcgggt gatggcatcc
     1681 gcgccgcggt tttcggcaat aaattcactc atggtgttac ccgtggcatg tttcagggga
     1741 aaagccgacc actttaacat tgaaatgtgt gacgaattta acgtttttgc aacttttacg
     1801 gtgttgcttg agctggagtt aagatcgaaa cggaacaggg caaagattac cggatgcggc
     1861 gtgaacgtct tatccacccg acaggtcagg cgcggtctgt aggctgataa gacgaaacgg
     1921 tttcaatgca accagtgaac gccctcggca ggctgaaaga acgcgttata gcgcatctga
     1981 aaagcattaa tatcctgcat ttcaggttcg gtttcacgca gaaaacccag cgccagcctg
     2041 acctgagcat cggtaatcgg cgcggaaagg cgggcttttt gtaaaatccg gtgccattgc
     2101 agcaggttct gttcgctatc atcgacaatg acaatttgcc agataagcaa cacctgcgcg
     2161 gcgttttgca ggtgcgactc atccgggcga tgcaaataac gtaaaaattc gtcaccggct
     2221 gactttccca tttgatcgac catcgactcc acgggtacga catcgatatt cagccgatcg
     2281 ctgagacggc gaatcgcttt tctggccccg gatgtcagca cccgaaaacc aaccacaaac
     2341 actaccgcca gcgtggcgag cattatccag atcatgagtt ctcctgcgta atcagaactc
     2401 atgatatctg gaaaccctcg ccagttacta atccaccaga ataatcttca atgcgaacag
     2461 agctgcgacc accaccacac acaggttcag atcgcgccag cgcccggtgc acactttcat
     2521 gatgcagtac gacataaagc cgagtgcaat cccttcggtg atcgagaaag taaagggcat
     2581 catcaccgtg gtaataaacg caggcaccga ttcggtaaaa tcatcccagt taacgcgcgc
     2641 caggctcgaa gtcatcagca cgccaacaaa gattaacgct ccagcggttg cgtaaggagg
     2701 aaccatcgcc accagcggtg agaagaacat aactaacagg aacataacgc cgaccacgac
     2761 agccgttagc cccgtgcgac caccgactgc cacaccagaa gtactttcaa tataggcagt
     2821 aacagacgag gtaccgataa atgcgcctgc caccgaactg acgctatcaa catacagcgc
     2881 cttattcata ttggggaatt taccgttgct atctattaag cccgctttat cagttacacc
     2941 aattaatgtt cctgatgaat caaataggtt gatcagcata aaggagaaaa tgataccggc
     3001 gagttcaagt gttaacgcgc cgctcaaatc tacttcacca atgacgccgc taatatcagg
     3061 cggaatggaa tagacgccgc taaaatgaac atcaccgaaa aataatccac agcaagacgt
     3121 caccacaata gaaaccagca ccgcggcatg aaaatgacgt gatgacaaca cggttataat
     3181 aaaaaaccct aaaataccta acaacacgcc gtgagaactt aaatcgccaa tcatcaccag
     3241 cgtgtcttta ttggcgacaa taacaccagt gttttttaat cccattaagg caataaataa
     3301 tccgattcca ctggtgatac caatacgtaa acttaatgga atgttggaga tcatccagta
     3361 ccggatacga aacagcgtga gcaaaaatag cccaattgcg ccccagaata tcgcgcccat
     3421 cccggtctgc caggagatcc ccatcgcccc cacgaccacg aacgcaaaga aggcgttcag
     3481 ccccattgcc ggagccagcg ccacgggtaa gttagcaaat atccccatcg caatactgcc
     3541 gataccggca atcaaacagg tggtaacaaa caccactttc gggtccattt gtgccgcgcc
     3601 gaggatttgc gggttcacaa aaacaatgta caccatggtt aaaaaagtgg tcatccccgc
     3661 aatcaattct gtgcggacgg tggtcccatg ctgaggtagt ttaaataatc gcgaaagcgt
     3721 ccctgattca ttactcacgt aatcggtatt gtcattattc atttttttgt ccattggagg
     3781 agatttaatc ccttgctttt gtaaaacaaa tgacaacaac ggttcagtga taattatcac
     3841 atttcaattg cacattaata gatattcttt aataatctcg cgacgtttct ttatgataaa
     3901 taataatcaa attgataaaa tcaaaatgag aaaaatatga ataattccat taaccataaa
     3961 tttcatcaca ttagccgggc tgaataccag gaattgttag ccgtttcccg tggcgacgct
     4021 gttgccgatt atattattga taatgtctct attctcgacc tgatcaatgc cggagaaatt
     4081 tccggcccaa ttgtgattaa aggacgttac attgccggtg ttggcgcaga atacgctgat
     4141 actccggctt tgcagcggat tgatgctcgc ggcgcaacgg cggtgccagg gtttattgat
     4201 gctcacctgc atattgaatc cagcatgatg acgccggtca cttttgaaac cgctaccctg
     4261 ccgcgcggcc tgacgaccgt tatttgcgac cctcatgaaa tcgtcaacgt gatgggagaa
     4321 gccggattcg cctggtttgc ccgctgtgcc gaacaggcaa ggcaaaacca gtacttacag
     4381 gtcagctctt gcgtgcccgc tctggaaggc tgcgatgtta acggtgccag ttttaccctt
     4441 gaacagatgc tcgcctggcg ggaccatccg caggttaccg gccttgcaga aatgatggac
     4501 taccctggcg taattaacgg gcagaatgcg ctgctcgata aactcgatgc atttcgctac
     4561 ctgacgctgg acggtcactg tccgggtttg ggtggcaaag aacttaacgc ctatattgct
     4621 gcgggtattg aaaactgcca cgaaagttat cagctggaag aaggacgccg gaaattacaa
     4681 ctcggcatgt cgttgatgat ccgcgaaggg tccgctgcca gcaatctcaa cgcactggca
     4741 ccgttgatca acgaatttaa cagcccgcaa tgcatgctct gtaccgatga ccgtaacccg
     4801 tgggagatcg cccatgaagg gcatatcgat gccttaattc gccgcctgat cgaacaacac
     4861 aatgtgccgc tgcatgtggc atatcgcgtc gccagctggt cgacggcacg ccactttggt
     4921 ctgaatcacc tcggcttact ggcacccggc aagcaggccg atatcgtcct gttgagcgat
     4981 gcgcgtaagg tcacggtgca gcaggtactg gtgaaaggcg aaccgattga tgcgcaaacc
     5041 ttacaggcgg aagagtcggc gagactggcg ctgtctgccc cgccatatgg caataccatt
     5101 gcccgccagc cagtttccgc cagcgacttt gccctgcaat tcacgcccgg aaaacgctat
     5161 cgggtcattg acgtcatcca taacgaattg attacgcact cccgctccag cgtctacagc
     5221 gaaaatggtt ttgatcgcga tgatgtgtgc tttattgccg tacttgagcg ttacgggcaa
     5281 cggctggctc cggcctgtgg tttgctcggc ggctttggcc tgaatgaagg cgcgctggct
     5341 gcgacagtca gccatgacag ccataatatt gtgatgatcg gtcgcagtgc cgaagagatg
     5401 gcgctggcgg tcaatcaggt gattcaggat ggcggcgggc tgtgcgtggt acgtaacggc
     5461 caagtacaaa gtcatctgcc gttacccatt gccgggctga tgagcaccga cacggcgcag
     5521 tcgctggcgg aacaaattga cgccttgaaa gccgccgccc gtgaatgcgg tccgttaccc
     5581 gatgagccgt ttattcagat ggcgtttctt tctctgccag tgatccccgc gctaaaacta
     5641 accagtcagg ggctatttga tggcgagaag tttgccttca ctacgctgga agtcacggaa
     5701 taataaaaaa agcccggcgt catgcagggc aaaagtcacc agttacgttt atgccactgt
     5761 caactgctga atttttttct cgcggcggat tttgcgttct tccattaccg ccactatcgc
     5821 catcaggcag atacaaccaa tcgcggcgat atccagcgcg gcgaaggtgc ctgcccagcc
     5881 ggtaaggccg aataccggcg tcccatcggc aatcattccc agacctaact tggcaaagct
     5941 gtcaccaatc aggtaagcaa aggtgccttt aataccatcg gcagcgccaa tcgctttttt
     6001 aggtacaaag ccaacagcag ccacaccaat caacaattgc gggccaaaga ccaggaaacc
     6061 caacgcaaag agagaagcca gatagatata ttcgttactg gcatgttgat acacaccgag
     6121 cgtggcgata atcagcgcca gcgcgatgca ggccaccagg ccacggcgac cgttcgccag
     6181 gtcagagagc cagccccaca gcagcgtacc gaccagcgca ccagcttcaa acagcgtaaa
     6241 gccctgaatc gccaccgctt tagagagttt cagttcctgg aacgcgtata cggttgacca
     6301 ctggtcgata ccaatacgta ccacatagag gaaaatgttg gcgaagcaca gcagccagat
     6361 cactttgttt ttcagcacat actcaacaaa gatctgccac ttggtcatat cggtagattc
     6421 tgtctctttg tcctcttcgc tgatctcctc gccgaacagt tcttcagctt tgccgaggcc
     6481 ataagattcc ggggagtcgc tgccgtaacg caggccgata aaaccgacaa tcagcgcgat
     6541 aatcgacggg aagataaaca tgccgatgac atggccatcg aacaggtaat ttgccccgaa
     6601 cagcgccaca cctgctgcgc ctgcaccgcc aaggttgtga gaaatattcc agaaaccgag
     6661 gaatgtcccg cgtttacgac gcggcgtcca tttggtgatg gtggagtaac tgcacgaacc
     6721 gccggtactc tggaaaaagc cgcttaaggc gtagaaggca atcatcagga acaggctaac
     6781 cgagccgctg cccatactgg cactgaagcc cagcatacaa atggcagaga ggataagcat
     6841 gaacggcagg aattgtttgg tgtttttgcc gtcggcgtag tatgaaacca acgttttacc
     6901 cacgccataa gtgatggaga aacccaggcc gatcatcccc agctgcgtca tgctcaaccc
     6961 gtaggtcgaa atcatatcgt tctgcgcgat gttaaagttc ttgcgaatca ggtacatcgt
     7021 caggtagccg ataaagacca ccaggtagga ttgcatgaac ggtttgaacc acattttgcg
     7081 ccgcacttcg agcggaaggt ccagggtcgg cttgcgaacc tggtttagga aagccagcat
     7141 gggttactcc tgaaatgaat acctgcgtga ggcatgcatt gtaaaaatca gcagcaaatc
     7201 aggcctgaga tagcgtccaa gaaaaacctg gaaaatttct tagttttgcc ggatatgaag
     7261 tgaaaaggtg agatgcatca cgcttcacgc ggtgtctggg cgttcaaaaa gggcagtaac
     7321 agcagtgcgg aaatcccggc ggcgatagag ataaccacaa aaaatccgct ccagtgccag
     7381 gtatcgagca ctttcgccag cggccaacca gcaagcgacg cccccagata agcaaacaag
     7441 ccgacaaacc ccgtcgccgc ccctgccgcc tctttgtggg aacactctgc cgccgccata
     7501 ccaattaaca tctgcgggcc aaagacaaaa aaaccaatgg tgaagaagca ggttgcctgc
     7561 atcacgtagc tggcaaatgg catcagccac agcgagccga ctgaaagcaa aattccggcg
     7621 gcgaaaatca aattcatcgg cccacggttg ccgttaaaca atttgtccga gccccaaccg
     7681 gctaccagcg caccgataaa tccgcccagt tcaaacatcg tcactgccgt attcgccgtg
     7741 accagatcga cgcccagcgt ctcggacata tacaaattgc cccagtcgtt gatcgccgcc
     7801 cggaccacat agaccagcac atagcaaaac gaaagcagcc agatatacgg attcagcaac
     7861 acatatttgg tgaggatctc tttacgcgtc aaccctgccc cttcttgttg ttgagcaatt
     7921 tccagcgcgt cgtgccgcca ttcaccgacc gccggtaaac ctaacgcctg cgggcgatcg
     7981 cgtagccgcc agcagagaaa aatccccacg actatcgcca tacaaccagc aatcatcatc
     8041 ccggcacgcc agccgtaatg cagcgcactc gctgccatca caatgggaat gagtgcgccg
     8101 ccgacgttat gcgccgtgtt ccataatgcc caccaaccgc cgcgctcggt acgtgaatac
     8161 caggccgtta acagacgcgc acacaccggt gaaccccagc cctggaaaaa ggcgttcagc
     8221 acccagagca cggcaaacgc ccatagcgac gtcgagaagc caaacagaat gttcatgatg
     8281 cccgtggcga taagccctat ccccataaaa taacgggcat ttgagcgatc gctgacaatg
     8341 ccggagacaa acttcgacac gccgtaagta atgtaaaaca gggtcgctaa caggccgata
     8401 tcgctacggc tgagcacgcc gttagcaagg atttctggta ccgcggcgtt aaaacttttc
     8461 cgcgtgaagt aaaacagcgc gtaacccagc cagatggtca gcagaatatg ccgacgccag
     8521 tagcgataac gggcatcaat ttcatgttta tcagtcatta atggcgcatc ggcaggcgct
     8581 ttcagaaacg gcaacatcat cgaaccttag acataacgtt gaggtagaga aacgctgaca
     8641 cgcgtgccgt gcagacagga aatgtgtaat gtgccgccca gcgccgttac gcgctcgcgc
     8701 attccggtga ggccaaaacc ttgttgcccg gaacccggcg gcaaaccgct gccatcgtct
     8761 tcaatcacca gcatcaaccg ttcatcctgc tgccagcctt gcagcgtaac cgcgctggca
     8821 tcggcatgtt tcacaatgtt gttcagccct tcctggcaga cacgaaacag cgtcacgcgc
     8881 tggttttcgc ttaatgctga ttcatcgata cgccattcga gatggctgac aatgccgcgc
     8941 ccttccagct ccatttcccg catcagtgag cggatggcct gctccagggt gagatcatcc
     9001 agctggcgcg gacgtaaccg tcctaacaaa cggcgcaccg cgtcgtaaac gcccagcgac
     9061 agttgttcga tgagctgccc gctctgcttc acactggcgt tatctgccgc cagccgctga
     9121 acaatgcccg cctgagtacg aatagcagtg atggtctgac cgatatcgtc gtgcagctca
     9181 cgcgccacgt cgcggcgcac gctctcttcg gtttctaaca aacgttcagc cagatgctga
     9241 ttgcgcgcca gttccttttg tagcgactgg ttaagttcac gcaaccgctg gatgcctgcg
     9301 cccagtaaca accctgtcag actttgcacc agcaacgaga gtaataaatc caccagatga
     9361 tcgcgccagg tttgactggc gatcagcgcg atggcgttca tcaacgtcgc aatcagcgct
     9421 ccttgccagc catagtgcca ggccagcgcg ataatcggca gcgccagaca gaatggcgta
     9481 aagcgcgaca gttcgtccgg caatcccaac tggagccaga gactgataac aaacagcagc
     9541 aagtaccaga ccagatgccg cccgcgccag ttgatgggct gagaaaccag tgacgggccg
     9601 agcggcagcc aggtgttatt ggcgagatag tgccagaaca ccagacatat cggggccagc
     9661 gtcagaccgc cagttaaagt cagcaacagc gcattccacg actctttgcc gtgccaaagc
     9721 cagggcagcg actgcaacaa cgccgccgcc gttaacgccg caccctgtaa cagcaaggtg
     9781 cgccagtcac gctgatggcg atagcgcgag atcagcgcta cgggcagtaa cgtcagtaaa
     9841 ctaccgatca tcaataacgg aaaatgggtt aaaccgaccg cctgcgttag ccagtaaatc
     9901 agcagccact ccgcacccag caatacgggc cagtatccgc gcgggcattg cagcattagc
     9961 cccagacgca gaccaaacgg aaataacagc accgccatat cagggcgctc aaccagatgc
    10021 aggctgatac tccataggca aaaccatgcg gcagagaaga taaaaaagca ggcaataacg
    10081 gtaattaagc gggagaacaa cgtcttcatc accagccatc aaacatgcgg cgcgccagct
    10141 ctacgtcgtt actgacgccc agtttttcca tcagattggc gcgatggacg tgtaccgttt
    10201 tcggtgacaa gcccagttcg gcggcaatct ctttcaccgc cattccttgc gccagttttt
    10261 ccgccacctg gcgttcacgt ttggtcagcg ggtcctgacg accggatgcc agtttaatgg
    10321 caatatccgg cgtcagataa cagccgcccg tggcaaccgt atgcaccgca gcaatgagtt
    10381 catccgggct acagcgtttg gaaagaaagc cgcgtgcccc cgcgttaagc gcctgctcaa
    10441 ccagcgcagg actgtcgtga acggagagca taatcgtcgc catacctttc ggcagctggc
    10501 ttagcagctc cagaccggag atatcgggca tggagatatc gcaaatacac acctgcacac
    10561 cgcgccccgg cagccccgcc agcgcctcgc gccccgaacc aaactcggca actacctgca
    10621 aatcaggttc cagccccagc agctgcgcaa agccggagcg gacgatgagg tgatcgtcta
    10681 taagggcaac ggtgatcatg gtcttgtcct ggcgggtaaa aaaaaacgcg cttaccttaa
    10741 cgataagcgc gatgttgttc aagccttgag cggttactga aaaaacaccg cgatcttgtt
    10801 aaacatcgtc ggatcggact gattacgctg cactttcacg acatcttcca gcttagcgat
    10861 ttggcttatc atctgctcca gacgctggtc gtcattgacc agtagccaga tatggctttt
    10921 gtcgctgtcc tgaatcggca gacaaagaat gccttcaacg ttaaaagcgc ggcgggcaaa
    10981 aaggccacaa acgtgggtca ttacgcccgg atggttgcga acggtgagct ccagaattac
    11041 gttgttatga gttgtgtttt gcatggctta ttcccccacc atttcagtat tcgccgcacc
    11101 tggcggcacc atcgggtaaa ctttttcttc ggcatcaatg cgcacatgga tcagcgccgg
    11161 gccagggcga ttgatgattt cctgcaatgc agcctgcgga tcggcttcgt tattcaaatc
    11221 acaggtttcg aggccgaatc cggcggcaat ctgcataaag ttgattttgc ccggataggt
    11281 ggcggcaaaa acgccttgct cgtagaacag actctgttgc tgatgcacca gccccagcgc
    11341 ttcgttgttc atcagaatga ttttgacatc cagctgattt tcactggcgg tcgccatctc
    11401 ctgaatattc atcatcaggc tgccgtcgcc ggagaaacac aacactttgc gatccgggtt
    11461 cgccagcgcc gcgccaatcg ccgcaggcag gccaaaaccc atcgtgccca gcccaccgga
    11521 ggtcagccac tggcgtgggc gattgagcgg ataagcttgc gcggtccaca tctgatgctg
    11581 acccacatcg gtggtgataa tcgcattgtc atcgacacag gcggcaacgg cgttgatcag
    11641 gccgtaatgg cttaatggat cgcacgcttt cgggattgga cacgggaatt cgcgctgcaa
    11701 atccgctacc aactggtgcc actctgcacg cggttgcgct tccacccgcg ggatcaattg
    11761 cgccagcacg tcatcaacat ccgcctgaat cgccacatgc ggctgcttga ttttacccag
    11821 ctctgcgcga tcgatatcga catgaataat tttggcattc ggacagaact gctcggtttt
    11881 gccaatcgcc cggtcatcaa aacgcgcacc gagcacaatc aacaaatccg cctcctgcaa
    11941 aatatagttg gtactgcgca cgccgtgcat ccccagcata cccagcgaca acggatgcgc
    12001 ttttggcagc atgcccagcg ccattaaagt catggtggta ggcagttgcg ctttctccgc
    12061 cagttcacgc acccgcgcag gcgcattgat cacaccacca cccagataaa gcaccgggcg
    12121 tttggcagcg ttaatcattg tagctgcgtc acgaatgctt tcttcgctaa aggcgggtgc
    12181 agcggctttt tctgccaccg cgggctgagc ttcaatctca aaaaccgccg tttgtacatc
    12241 cttaggaatg tctatccaca ccgggctagg gcggcctgat tgcgcaatgc ggaaggcatc
    12301 gctcatgacc tgtgggagct cttcgatatg tctgaccaga tagttgtgtt tggtgatggg
    12361 gatagagatg ccgtaggtgt ccacttcttg gaaggcgtcg gtgccgatca tcgaggcggg
    12421 aacctgacca gtgatgcaaa tcagcgggat ggagtccagc cgcgcatcag caatggcggt
    12481 caccaggtta gtcgcacccg gcccgctaca ggccatacag accgccggtt taccgtcggt
    12541 gcgcgccatt ccctgagcga taaaccccgc gccctgttca tggcgagcca gaatatggcg
    12601 gatttgcgtg ctttggctta aggcgtcgta aacaggcagg atagaaccgc ccggaatgcc
    12661 cgtcacaatc ttaatgccct gctgttccag gaaatgaacg ataaattctg cgccggtaaa
    12721 gcgcttacgc gtcgatgttg tgcccgaact tgccatgctc cagtcctttt cttctgggcc
    12781 gactttccgg gaggtgctta aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg
    12841 aatcgtgtgt tgttccagtc cctacggcgc attgccgacg accaccacca cacgcacgac
    12901 gaccactgcg gcggatggcg cagttggtag tagttttgcg ttgaacatgg aagtagtcat
    12961 tggggacctt gttggttttg tgtttaacaa tatttataca agcacagctt tacaggggag
    13021 acaatggaaa atttttcagc aagggaaaat tgaggggttg atcacgtttt gtactgaatt
    13081 gcagataaca aaaaaccccg ccggagcgag gtttcgtcag ccgcctgcgg ctggtaaccg
    13141 caaagcacac tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat
    13201 ttcggcacga attttgacgt atttagtgca tagttgagta tcgttcacag tttgcgtttt
    13261 gtccaaatat tactgtttat ttatacagta aacttctata atatcactgt acgcaatgtg
    13321 ttatgcgggg gccgcatcgt tacccggcgc actaagtcct ggctgaaacg ggtggtgccg
    13381 tcagcgcctt aaccccgcgt gagcacactg tgttatgtca acaagcacaa cgtttctcct
    13441 tgagataccg cgtgcacaac agctggcaac aggcagcgga aaggtacgtc agctggcagt
    13501 gctcctgaac cacaggagac gcgtatgagc ctggtggata tcgccattct tatcctcaaa
    13561 ctcattgttg cagcactgca actgcttgat gctgttctga aatacctgaa gtaattcaga
    13621 ttcaagtcgc accaaagggg agcgggtaac cgctcccctt ttatatttag cgtgcgggtt
    13681 ggagtcggat gcgatgctga cgcatcttat ccgccctacc atccctcccg gcaacattta
    13741 ttgccgcttt tgtttacata ttctgccgct aaacaattcc ccattcctgg cgtatatctg
    13801 gctaacattc atcaatgtga tagattcctc tcccgcattt atgggaatgc gtagtgactt
    13861 attctaatta tttttataaa agcatccgtg ataatgaaaa ggcaaagaaa cgtcaatttg
    13921 ttattgatgt tggtattact cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca
    13981 gctattgccg atatggcgcg cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg
    14041 ggcgcttatc tgctgactta cggtgtctca cagctgtttt atggcccgat atccgaccgt
    14101 gtcggtcgcc gaccagtgat cctcgtcgga atgtccattt ttatgctggc aacgctggtc
    14161 gcggtcacga cctccagttt gacggtattg attgccgcca gcgcgatgca ggggatgggc
    14221 accggcgttg gcggcgtaat ggcgcgtact ttgccgcgtg atttatatga acggacacag
    14281 ttgcgccatg ctaacagcct gttaaacatg gggattctcg tcagtccgtt gctcgcaccg
    14341 ctaatcggcg gtctgctgga tacgatgtgg aactggcgcg cctgttatct ctttttgttg
    14401 gttctttgtg ctggtgtgac cttcagtatg gcccgctgga tgccggaaac gcgtccggtc
    14461 gatgcaccgc gcacgcgcct gcttaccagt tataaaacgc ttttcggtaa cagcggtttt
    14521 aactgttatt tgctgatgct gattggcggt ctggccggga ttgctgcctt tgaagcctgc
    14581 tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta tgacggtcag tattttgttt
    14641 attctgccga ttccggcggc attttttggg gcatggtttg ccgggcgtcc taataaacgc
    14701 ttctcaacgt tgatgtggca gtcggttatc tgctgcctgc tggctggctt gctgatgtgg
    14761 atccccgact ggtttggcgt gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc
    14821 tttttcggtg ccgggatgct gtttccgctg gcgaccagcg gcgcgatgga gccgttcccc
    14881 ttcctggcgg gcacggctgg tgcgctggtc ggcggtctgc aaaacattgg ttccggcgtg
    14941 ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg gcagcctggg gttgttgatg
    15001 accttaatgg gattgttgat cgtgctgtgc tggctgccgc tggcgacgcg gatgtcgcat
    15061 caggggcagc ccgtttaagc gcacgtcacc gcagcatcgt catcagctcc atgggagaac
    15121 gatgctgctt tatcagatca cgcatcaccc gcatatgcgg tgcggagtaa gaataaaacg
    15181 cctgatagcc cgcacaaagc acgctgtctg gcgtctcctg ccgagcgtgc gggcacaggc
    15241 gatgccagtc gcacgcctgg cagtcacggc gcggttgcgg gttttcgctg ccacaccatt
    15301 tttgtaaggt ggcgtcaaaa agctcaatcg agatacgact gatatctgcc cgcacccaac
    15361 actcaaacag ggcaattaaa aatttccccc acagctctgc tgtcaccgaa gcggccgtca
    15421 gatcgccccg gtcatcaaat tcaagatagg gaatgagctg tacggagcgg atgccccgct
    15481 gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc cttgtactct acaaccagct
    15541 tatgcctgtc ctcttcagca tcaatgacct gacttcctgt catgcgactc tctcaataaa
    15601 tacgataagt tgatgtatat gcgttacagc aaacagtatt gcgagagata acaccgcgag
    15661 ggagatcaaa aatttgtcac ggactacgtg aaattgggaa aaatcgctat tcgtgacatc
    15721 cattaaattg cgattacggg tgtagtgcca gaggatcagc gccacgatgg cgaggatgcc
    15781 aatcgaaatc cagaataaca tgcccgcctg gtgccagttg tgtttgatag ccaacgccat
    15841 caacgcgccg tagcccagca tggtacgaaa ccacgccagt gatgtgcgct ccggctgcag
    15901 ccctgggtcg gcaatgcgtc tggctttacg gctatccggc atacaacacc agtcccataa
    15961 cgatcaccac cacaaccatc aatattaagc tgatgattaa taagctgttg gtgtagggca
    16021 ggtcctcttt cagacgcatc gctttttcat tgcgtaacca gcgcaggtag ccatacattg
    16081 ctaacccgcc ggaaaacaga cacaacaaca acgccagcag ttcacgaatg acgggcgtgg
    16141 cgaaatctgg cgcaagttga tcaagcccaa cccccgccgc cagaaatcct agtgcggtac
    16201 ggatccacgc cagaaaagta cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc
    16261 caaggcggga aattttcatt gttgctcctt tgcaatttca ctaacgctct atctttagcg
    16321 gtttttttta agaatttcgt ccgcccgttt cctgatatgc ttttgccatc cgctacaagg
    16381 aggtcagatg ggtatcatag cgcaaaataa aatcagttca ttgggcatgc tgttcggtgc
    16441 catcgcttta atgatgggga ttatccactt ttcattcggc cctttttccg caccaccgcc
    16501 aacgcttgaa agcatcgtgg cggacaaaac ggctgaaatt aaaagaggtc ttctcgccgg
    16561 aataaaaggc gaaaaaatta ccactgtgga gaaaaaagag gacatggata tcgataaaat
    16621 cctcgatcaa agcggtatcg cactggccat tgctgcgcta ctttgcgcat ttattggcgg
    16681 tatgcgtaag gaaaatcgct ggggcattcg cggcgcgctg gtttttggta tcggcatcgt
    16741 gtgcagcatt ttgctgatat tcctgatttt ttccttcctg accggcggtt cgctggttta
    16801 agcagcctga ataatgcccg ccgggtgatc atcagcgggc attatcgtca gaatttctgt
    16861 tttttctcct ccaccttcac tccttgcgtc ggtaggccgg tgtcatagtc gcgtaccact
    16921 ggcgaatagc catcttgtgg gcgtgggcga aacgccccca tccagcgcgg tcgtgcatct
    16981 ttacgccacg gacgcagact ccattggtaa ctgcggaacg gatcgcgaat tttgtccatg
    17041 taatccaata aggcgtcatg cattttgctg cgaacgtctg caaaacggat atcatcgatc
    17101 aggttatgca tttcatttgg gtcattacgg cgatcgtaaa gttcatcact ggtgaagagg
    17161 ttgagcacca gtttaaagtc atccgtcacc cagcaacgca ccggaataaa accgccaaag
    17221 ctgtcatgct caatctcgta gcggttaaat tccaccatca cgccgcgtgg ctctttcacg
    17281 gcaaggatat tttcccccgg cagaatctct ggtttttcaa tatctgccag cgccatcatt
    17341 gtcggcagta aatcaatatg actgactggc gtatcgacct gtcgccgctc cccttgcggc
    17401 gaacggatga tcagcggaat acgggtgatg tcgtcataca tcgccgctcc tttactgatc
    17461 agcttatgtg cgcccatcat ttcaccgtga tcggaggtat aaatgaccca cgtattttca
    17521 cgttgctctg gcgttaaggc attgatgaca cgtccgattt ggtcatcaac aaagtcattg
    17581 caggcaaaat agagcggatg gtgataaagc ccgtcatcac cgactggcga tggcatcgcc
    17641 tgcgcccata agcggtgatg ttccggtttg ttcgccaggt catcctgtga tttctcgcca
    17701 agttcgtagt aaaaatcagc gtatttctct aaatactcca ccggacaggt gaacgggtga
    17761 tgcggctcat catacgaaac caccatcagg aagggttcct cggcgcgcac gggctattgc
    17821 agaaaatcta ccgctcgatt gctgatgcgg tgcgcccagg tgaaggtttc gtcaatatgg
    17881 ttcgcctgta aatcttcgac gctgtttagg ccattgcgcc acaggctgat ctctttttcc
    17941 gtcagttcgc taagatagtt cgccccatca aaccagtaat cagcatccca ctccggcgga
    18001 cactcgccag tgccgaaata gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg
    18061 tgatagccgg catccttaaa gtagcgcccc atagtggaga tgtttttgcc tggcgcgacg
    18121 ttgttggtcc acgggccgga ctggttagcg tagataccgg tgaatagtcc ggcccgtgca
    18181 ggcgtacaaa ccggtgaaca agtgtaggcg gaattaaagc gaataccttc cgccgccaga
    18241 ctatcaatat tttgcgtatt cagcggttta ccgctatagc aaccgaccat attggtggcc
    18301 tgggtatcgg tcaattgggg cgtttcatag gctatccttc gcgtcgggag taaaggttac
    18361 ggctgggtcg tgacgatgcc accagctgtc aaaaatcagg tagacaatca ctacggcagc
    18421 gatgaaataa ctgatcatcg ctaaccagcg cgtaccgtag ccgccgaatt cagccagccc
    18481 gacatagacg ccaatcatcg cgaacaggat accaattgac gcgatcttga cgtttttcca
    18541 cggtttcatg tccaccgcaa acgcatcttt gaaggtgaac ggcgtggcgc gcggtttgat
    18601 aaaaccgatc accagcatca cgaccacgtt gatgcagaac gtacaggcca gcacatagag
    18661 gaaatggaag tcgaacttca ccagatagtt gatggtgatg tagctgatta tgccaatccc
    18721 catcgccact tttgccgcca gcgccgggat gcgcggaaag aaaaagccca taatgatgat
    18781 ggtaaccagc ggcacgttgt aaatgccgtt gagctgtttc atccagctat acagcccctg
    18841 cggcgcgttg gcgatccacg gcgctaccaa aaccgaaacg atagcgataa agaaaccaaa
    18901 tttgcgcccg acggtgacca gctgctgcgg ctcggcattc tggttaatga tgcgacggta
    18961 aatacccata ctgaataatg tactggcgct attcagaaag ccgttgaagg tactgatcac
    19021 cgcaccaaat aacaccgcgc cgaagaaacc ctccagtggc actggcaaaa cgttattgac
    19081 cagcgtcggg taggccatgt cggctttcgg tagatcctga tacagatgaa acgcaatcaa
    19141 ccctggcagc accagtacca gcgggtcgag cattttcagc accgccgtta acagcgcccc
    19201 cttttgccct tccgccaggc tttttgacgc cagcgtgcgt tgcacgatgc cctgatttgt
    19261 acaccagtaa aaggtgttca ccagaattaa accggtaaat gccgcgccaa tcggcaaggg
    19321 atcggtcggg ccaccgactg agtttaattt ctcggcgtga acggtggtaa tttgctcaat
    19381 gccctgcata aagctgccct tgcctatcgc gatcaggcca aataccggca ccatcaaccc
    19441 gccgataacc agcccaatac cgttgatgga gtccgccact gccattgcgc gcagtccgcc
    19501 gatcaccgca tacaaaattc ccgccagacc aagcaaaatt accagtagcc agatagccgc
    19561 accgtgagaa atctgtagcg attccccgac gtgaaacagg ctgttgagcg ccaacgcgcc
    19621 ggagtagaga acaatcggca gaaagcagac gccggtggca atgaggaagc agaagtcgat
    19681 gataatacgc gtcgttttat cataacgttc ctccagaaaa tcggggatgg tggcaatgcc
    19741 gcgcttcaga tagcgcggta aaaagattag cgcgaggaag atcagcgtca ccgctgaagt
    19801 cacttcccag cccatcaccg acatgccgct tttgtaggcc tgcccggaaa gaccaaccag
    19861 ttgttccgtg gaaaggttgg ttaacattaa cgaagcggca ataaccggcg cttttagtga
    19921 acgtccggca agaaaatagc cttgttgcga tccggtatct gttttgcgaa ccttccacca
    19981 ggtgatcacc gccaccagca gcgtaaaacc gacaaaactc aagatttgta acgaattcat
    20041 ctcatagccc ttatttatgt ttatgtcccg gacgcatgaa tatagggaga ggtaaaatgc
    20101 gtcgggtcag gcagtcagat tcttaaaata caaacgtcgt atccctgaac ggattgtttt
    20161 ctgttaagtt caggttgtga gcataattcg ccggagggat attaaaatga atggaaaatt
    20221 gcaaagttca gatgtaaaaa acgaaactcc gtacaatatt cccttattga ttaacgaaaa
    20281 tgtgatctcc agcggaattt ctctgatctc gctctggcat acctacgccg acgagcatta
    20341 ccgggtgatc tggccgcggg acaagaagaa accgcttatc gccaactcat gggttgcggt
    20401 gtataccgta caaggatgtg ggaaaattct tttaaagaat ggcgaacaaa taactctgca
    20461 tagcaactgt attatatttt taaagccaat ggatattcac tcttatcact gtgaaggttt
    20521 agtctgggaa cagtactgga tggaatttac ccccaccagt atgatggata ttcccgttgg
    20581 tcagcaaagc gttatttata atggcgaaat ttataatcag gaactcaccg aagttgctga
    20641 gttaataact tcaccagaag caataaaaaa taatctggca gtcgcttttc tgacgaaaat
    20701 tatttatcag tggatttgtc ttatgtccgc agatggtaaa aaagatccac aacggcggca
    20761 aattgaaaaa ttaattgcca ctttacatgc cagtctgcaa caacgctgga gcgtagctga
    20821 tatggctgcc acgatcccct gtagcgaagc ctggttgcgt cgtctgtttt tacgctatac
    20881 cggcaagacg ccgaaagaat attacctcga tgcgcgtctg gatctggcgc tatcgctatt
    20941 aaagcaacaa ggaaactcgg ttggcgaagt cgctgatacg ctcaacttct tcgactcctt
    21001 tcatttcagc aaagccttta aacataaatt tggttatgcg ccgtcagccg tgctaaagaa
    21061 tacggaccag cacccaacgg atgccagtcc acacaattaa cgtaactctg gccagtacgc
    21121 tttgttggcc tccagcaact catccagaat agctttggcg accgaggcgc tcggtaccgt
    21181 tttcgacaac gtaatcgcct gccacaggtg ctgatatgaa cgctgttccc aggcgtccac
    21241 gaccagtttt tccaccgcca cttgctgact cattaaccct ttctgaaact gcgggatatc
    21301 gccgaccacc agtggttcag gccgtagtag cgcacgcgca tctgtttgcg atcttgcagc
    21361 ccaacaattt gcgccatccg gctttcaata ccgattggca tgtcacagat gttgaggatt
    21421 ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa ttgctgccgg gttggagtag
    21481 ttgagcatct aggcgtttgg tgaatatttt tccatataat ccaccagttc caggacgccg
    21541 ccaatggaac gcatgccgta cgctattccg ccgggtccgc aagtttcctg accaacaaca
    21601 ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg ggtacttgcc gacgcggatg
    21661 tgcgccatga caaaatcaac gtcgctgaac gccacttcag gatcggtggt gtaactaaac
    21721 gcaatgtccg gcgctttttc tttaaggatg actttacagg cttcggcaat cacttcctgc
    21781 cgcgcgccat cgttatcata aaatttcagt gcacgaagcg ggaaacggtc ctgattcgcc
    21841 aggagcatca acacgatgcc tggcgtaaag gtgcttccac cgcctgcgac aaccactgag
    21901 aatttggtca ttatactgcc tccgtaatgg caacattttc tgctgattga tgagaattaa
    21961 ttaagctatc gagctgttca cgcaattggg atacatgcag accaatgatc acctgaatgg
    22021 catcgccact acggaagacg ccgtgcgctc ccagcttttt aaagacttcg tcatccagcg
    22081 tttgtgacat gtcatgcagt gcaatacgta gacgcgtcgc gcaattgtta atgctggaga
    22141 tattgccgac cccgcccagg gcttgcagga taccggcagc ctgatccagc tctttttttg
    22201 gctctgcagc ggtggtttgg cctcgcgagg ctttgtattc ggcttttgag tagagtttca
    22261 cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg cagaatcagg gtgcggaaaa
    22321 ccacgaagta cagcagggta aagcacaacc caatggcgat ttgggtcagc atcatatccg
    22381 cgtggttgct gaacatcggg atccagtttt gcggtaaaac ctggtcaatc agacctccgc
    22441 ccatgttgcc caccacacca aagagataca ttacggtcga cattgaggcc gccagcacgg
    22501 cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt aaattccagc ggttcggtaa
    22561 ttcccaccag catggcggtt aaggttgcgg gaatcagcaa gcccgccact tttacccgat
    22621 tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc cacggcacca aagatttttg
    22681 agttaccgtg cagggcaaaa ccgccttccg ggaacaacga tttcagtggc tcggcgctca
    22741 gactgaactc ttgcagatgc tgcgcccagt acatctgaat gccgccttca acagctgccg
    22801 gaccaaagat aaacggtccg tagatgaagt ggtgtaaacc ggttgggatc agaatacgtt
    22861 cgaggaaggt gtaaacccac accccaagcg cacccgccga acgcaggaac gcttgcagag
    22921 attcaatccc catttgtact tttggccagc cgagcaatgt cagccaggca caggggatca
    22981 tcaccaggaa ggcgatactc accacataag acgtcccctg gaaaatgccg agaaaaaccg
    23041 gcagtttttt atcgaacaga cggttatgca gcgccgtcac aatgccggaa atgataattg
    23101 cgccgataat gctggtatcg agggttttaa tcccggccat cattgtcaga ccgctacctg
    23161 ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc ccaggtcatt cccatcgcat
    23221 tgatgaaata gttccaggtc aggaaactca ccatcaccgc cagacaagca cgtccctgcg
    23281 cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat cagcggcata ttacggaata
    23341 ccgtccaacc gccctcttca ataatgtgta cgatttgcgc gaataaactg ttcggatcgg
    23401 tcagtgattc cccgacaaaa catcgggttt tgcagcaaga tggcaagacc caccacaatc
    23461 ccggcgaagg gaaacagcag cactggcgtg aacatcgcgc cgccaaagcg ttgaatttga
    23521 ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga ttggttatgc
    23581 ggcagacctg gagcgaggat aggaagatgc gatcggcaga acatgacgac ccaatgggat
    23641 tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg cgaaaaagta
    23701 gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag atttttttca
    23761 atcgattaag cagaacttgt gggcgcatta cccgtgcttg caggcaaaaa agagagctag
    23821 agatgatcta caaaagcatt gcggagcggt taagaattcg acttaattcc gcggatttca
    23881 cgctaaacag ccttcttccc ggtgaaaaaa agctggcgga agagtttgcg gtatcgcgga
    23941 tgactatccg taaagccatt gacctgctgg tagcgtgggg gctggtggtc cgccgccacg
    24001 gcagcggcac ttacctggtg cgcaaagatg tgctgcatca aaccgccagc ctgaccggac
    24061 tggtggaggt gttaaaacgg cagggaaaaa cggtcaccag ccaggtgctg atttttgaaa
    24121 tcatgcctgc gcctccggcc attgccagcc agttacggat tcaaatcaac gagcagatct
    24181 acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct aatgctggaa gacagctata
    24241 tgccggtaaa actgttccgt aacctttcgc tgcaacatct ggaagggtcg aagtttgaat
    24301 atattgaaca agagtgcggg attttgattg gcggtaatta tgaaagcctg atgccggtgc
    24361 tcgccgatag actgctggcg cggcaaatga aggtagcgga acacacgcca ctgctgcgga
    24421 tcacctcgtt gtcatatagc gagagcgggg agtttttgaa ttattcagtg atgttcagaa
    24481 atgccagcga atactaggtg gagtaccatt tacggcgact ccacccggaa aaaagttaac
    24541 cgatactcca gaagagcacc gccagtaatt ggggggtgat aatgcgcagg aacatcacta
    24601 acggatagac agtggcgtaa gagagcgccg ccgcaccact ggttggatga agattattag
    24661 caaacgccag cgccggagga tcggtcatgg aacctgccag catcccgcac atggtcaggt
    24721 aattcatttt ggctaacatc cgcgccagaa tgccaacagt aatcagcgga acggcggtga
    24781 tcagggcacc ataaccaatc cagcttagcc cttcgccatt gaccagggta ttcacaaaat
    24841 ccccaccaga tttcagacca acgaccgaga ggaacagcac gatccccagc tcccgcagcg
    24901 cgaggttggc acttggcggc ataaaccagt acagcttgcc gatacgcccg aggatcaacg
    24961 ccataatcag cggaccgccc gccagcccca gtttcaacgc ggccgggaat cctggcacaa
    25021 agacgggaat agaacctaac aatacgccaa gcccgatgcc aataaacact ggcagcatct
    25081 gaacctgttg cagtttttgc tgcgcattcc ccagcacatt ggcaacggca tcaattgcgg
    25141 acggacgccc caccaggttg aggatatcgc cgaactgcag gctgatatcg ccactggcga
    25201 ccagttcgac cccggcacgg ttcaggcgcg agataacaac gtcatagcgt tctttaaagt
    25261 gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt gaccaccaca cgctcgacgc
    25321 gcaaatcagt gcctttcgta gacagcgagg tatcgacctc ctgaccaatc accagttgcg
    25381 cattatgtaa atccgctggc tgccccacca gatgcagcaa atcgcccagt tggataatgg
    25441 tatctggcga aggtactttt agggtttctt ctcgtttcag acgcgagcag ataattttgt
    25501 cgccgttgag aatcggtaca tctttaatgg ctaaatcatg caggttaggg ttctcaacgc
    25561 gaatattgat agtcttgatc aacgcgccgc cattggtgcg tgaagactcg tgctgctgag
    25621 cttctgtctc gacattgacg cggaaaataa cccgcaacat ccacatggtg aacaaaatcc
    25681 cgcaaatgcc gaatggatac gccatcgcgt aactcatccc catctgatcg accatttcca
    25741 ttggtgtacc caggtcgcgc aagatctgct gccctgcccc cagcgctggc gtattggtta
    25801 ccgcaccgga gaaaatcccc agcactaccg gcagtggaat atcaaacagt ttatgcagga
    25861 tggcggtaac cagaccaccg atgatgacga tcagaacagc aaacaggttg aggcgtaatc
    25921 cggagacgcg caatgaggca aagaagcccg gccctacctg aatcccgatg gtataaacga
    25981 acaggatcag gccaaactcc tgaataacat gcagcatatc gctacttaat gtcatccccg
    26041 cctgagaaac aaaatggccg acgatgatcc caccaaacag cacgccgcca atacctaatc
    26101 ctatgccgcg aaatttgacg ttgccgataa acaaaccgac gactgccacc aaagccagaa
    26161 tactgaccgt taatgctata tcactcatcg tccatttctc ttgagataac attttaattg
    26221 ctacggatta tggctgaatc ggtgaagcag atttgcctta tagcgcacat tatcacggga
    26281 aagaacgtgc cgaaatatct taaacagtcg aaatttaacg cattgttgaa gaagaaagac
    26341 aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc tagttagcta tttaacgcgg
    26401 gacgttcgct gatagcgata cgctgcgctg cgatgggttc aggctcatta cgaattaaat
    26461 caatatgcag taaaccgttt acgaaggttg cgccagagac ttccatattt tcagccagcg
    26521 taaaactcag gctaaatggc tgattcataa gcccttgatg cagccatttt ttctcttctt
    26581 ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc ttccagttga atctctaaat
    26641 cttcctgacg gaaacctgcc agcgcaaggg taatgcggta gtggttatcg tcgcttttct
    26701 caatgttgta cggcgggaag ctctggcttt caccggcgtt ttgcagtgcg ttggccagtt
    26761 tgtcaaaacc gatccattga cgcatcagtg gggataaatc gaagttacgc atagtcattt
    26821 ctccttctaa gaagcgagta agtacctgca aatccgaaga ttcgcatatg ctccctgacg
    26881 gcgagcatgg agatgtcagg ccgcgccagg cggccttagg gaattagttg atttcgatac
    26941 ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc gatatacagc aaaccattta
    27001 ccaggttagc cccacgaaca tgaatgttct cagctaactg gaatttgcgt tcaaagttgc
    27061 gttcagcgat gccctggtac agataggtgc gctctttttg ttcgtcggcg tgagcacctt
    27121 tcaccaccag cagattatcc tgggcggtaa tttccagttc gctctcagca aaaccagcca
    27181 cagcgatagc aatgcggtaa tggttttcgt ctaccagttc aacgttatac ggagggtagc
    27241 cgccattact ctggctctgg ttgttttcta agtggttaaa caaacggtca aatccaatag
    27301 cagaacggta aagcggggat aaatcaaagt tacgcataat caatagctcc tgaaatcagc
    27361 gagaatgtaa gaccttccac aatggacagg tcaggtagcc agaacaccca tcaggcgcgt
    27421 cctcatcgac tacgatgtaa aatgggtctg gaaatgaact tttcaagcct tatcagcgga
    27481 cttttttttg cagtttatgg tctattgcat agactgaggg ggcagcacga aacgttaaag
    27541 tgcgatagcc gccacagtgc gacgtaatga tgatgttatt ttcagcaagg gatcgctata
    27601 actcatcagg ccaacaccca cgatcaacag atgaatttat tatgataaga aatgtgttgt
    27661 tagcgttcat gatatgcagc ggaatgacat tactcggagg atgctccagc gtgatgtccc
    27721 ataccggggg gaaagaagga acgtatcccg gcacccgcgc cagcgccacc atgattggcg
    27781 acgatgaaac caactggggc actaagtcgc tggcgattct cgatatgccg tttaccgctg
    27841 taatggacac gcttttactg ccgtgggatg tgttccgcaa agacagctcg gtgcgctcgc
    27901 gcgttgaaaa aagcgaagcc aacgcgcaag ccaccaatgc ggttattcca ccggccagaa
    27961 tgcctgataa ctgatttagc gatcagtttc ggtgatccac agctggccac cttccatcca
    28021 cgccagccat tgaccatccg gcgagaagac cacggcgtcc gcagaaggtg gattggcgtg
    28081 gttttcggtt aaatactcaa cctcgccgct ttgcgcatgg gcacaaacaa ttcgattatc
    28141 cagcacaaag cccaaccatt ctcctgacgg atgccagtta aatgcagact gaatatccgt
    28201 tttgttatgg gttaacttgc gcggttcgcc gccctgtggc gagataagcc acagttgcac
    28261 aatgccgtta tcatcacgca ttaaaaacgc gatttgcgta ccctgcggat tacagcgcac
    28321 ccagtggcgc gggacgttga ctaaccccgg ataagcccga tgatgggtaa aggttaaacg
    28381 tcgctgcacg acgccacgcg gtggcgcggg tagggttgtt tccgttccac ttaacggcgc
    28441 atcacctgcc gatttccagc tagcttcatc ttgcggtaac tcaacgataa atagctccgg
    28501 cactttctcg ccctttggcg aaagtgtgtc gccaataaac gccagcgcgt gatttcctac
    28561 ccatccttct tcataagcac gattgatttc atcactgcca ggctgcggcg tgggcgtggt
    28621 tttactcacc agcacacacc agtggctacc gctgtattca cgcggatgtt gcttttgtac
    28681 gttgaccggg ccaaacggtg cagcaacccc gacgtttcgc aaatccagcg ccggatcgag
    28741 ttgatgcatt acatggtcgt tataggtaaa gctccccctt tcaccgttcg ggctaaagac
    28801 atgcacatgg ctgccaccgc gcagcgctcc tggcgtatac ggcgcggtaa tatccattgc
    28861 atcgagattg ctcaccttgc cgccttcagc aatgactccg cgtcgatgat ggaagtcgta
    28921 atgccatgtt tcatcaggat tttcagggcc gtgaataaag acatatttct ctgacttcgg
    28981 atgaacggtc accacgccga cgtgtgcgcc ctgtgacgcg cgatagataa cctcgacctc
    29041 gcctgtatgg atattcacac gctcaatggt ctcgccggta aacgacgccc cagaaggacg
    29101 cacgtcaaat accagccact ggctgtcggg cgtccaggta ttggtattgg tattggtgag
    29161 caggtgatta cggggagcaa aggttatctg tttcatggcg cggtcctgat acaaaacggt
    29221 tcccgccatc atacttcaca aggacttcac tttcagacaa tagtcttttg ccatcgtgat
    29281 gaatgtgagt gaaaagatgg aacatttcga cgtggcgatt attggcctcg gcccggcagg
    29341 gtcggcgttg gcacgaaagt tagccggcaa aatgcaggtg atcgcgctgg ataaaaagca
    29401 ccagtgcggt actgaaggtt tcagcaaacc ttgcggcggt ctgctggcac cggacgcgca
    29461 gcgatctttt attcgcgatg gactgacgct tcctgtcgat gtgatcgcca atccacagat
    29521 tttcagcgtc aaaactgtcg acgtcgccgc atcgctcacg cgtaactacc agcgaagcta
    29581 tatcaatatt aatcgccatg ctttcgactt gtggatgaaa tcgctgatcc ccgccagcgt
    29641 tgaggtttat cacgatagcc tgtgccgaaa aatctggcgt gaggatgata aatggcatgt
    29701 catttttcgt gcagacggct gggagcaaca tattaccgcc cgctatctgg tcggtgccga
    29761 tggcgcaaac tcgatggtgc ggcgacatct ctacccggat catcaaatcc gtaaatatgt
    29821 cgctatccag cagtggttcg cagagaaaca tccggtgccg ttctactcct gcatctttga
    29881 taatgcgata actgactgtt actcatggag tatcagcaaa gatggttatt ttatctttgg
    29941 cggtgcctat ccaatgaaag acggtcagac gcgtttcacg acgctgaaag agaaaatgag
    30001 cgcctttcag ttccagtttg gcaaagcggt gaaaagtgag aaatgcacgg tgttgtttcc
    30061 ctcacgctgg caggattttg tctgtggtaa ggacaacgcc tttctgattg gtgaagcggc
    30121 gggatttatc agcgccagct cgctggaggg gattagctat gcgctggata gcgcagagat
    30181 tctgcgtgcg gtgttcctga aacagccgga gaagagcaac gccgcttact ggcgcgccac
    30241 acacaaactg cgtttaaaac tcttcggcaa gatagtaaaa agccgatgcc tgaccgcacc
    30301 ggctttaaga aagtggatta tgcgcagtgg tgtggcgcat attccacagt tgaaagatta
    30361 tccaacgcgc ttcacatcac ccaccagcag gatgtaagag agcgcgccaa tcaacgcgac
    30421 ggcggagata taaaccagtg caggcgcgaa accgtaaccc tgcgccaggt accccaccac
    30481 cagcggaacg gtgatgccgc ccagaccacc ggcgaagtta aacacgccgc cggttaaacc
    30541 aatcaggcgc atcggtgcca gagaagagac cagcgaccag gtaatcgaag caaaaccgtt
    30601 gccgaagaat gccagcgcca tcaggcacat aatcatcatc ggatcgttgg tgtagttagc
    30661 gcccataatg caggtggaga tcagcaagcc gcagataatc ggcgttttac gcgcaaagcc
    30721 cagtgaaaag cccttacgta ccagcagatc cgcgacccag ccagagagca ggacgccgac
    30781 aaacgccgcg aggaatggca ccgtggtcat aaagcctgct ttcagcgccg tgattccttt
    30841 ttcctgggtt aaatagttcg ggaaccaggt taagaaaaac cacagtgcag aagccaccgc
    30901 aaattgccca agataaacgc cgatcagttt acgatggaac accagtttcc agtctttggc
    30961 tgttaacggc tgacgcgcct ctttcttcac cggcgcatca ccatccacca gaccgccgcc
    31021 atcacgaatg taatccagtt cagctttgct gatacctttg gtcaggcgcg gcggctgata
    31081 aaccttaaac caaatcagcg accagataat gccgatacca ccagtgacaa tgaacaccca
    31141 gtgccagctc aacatctcct gaatccagat cagcagcgga gtcaaaaacg ccagaccgac
    31201 aaactgacca gacgtataaa aaccaacggc ggaagcgcgt tcatgttccg ggaaccagct
    31261 ggtcaccatc cggttattgg tcgggaaagc gggcgcttcg aaaataccgg ttatcgcacg
    31321 cagaccaatt aatgacatta atcccgtggc aaagccttgg aataaagtca ccactgacca
    31381 accaaatatc gcaataaaat aagtcacgcg agaacctacg cgatctaaaa accaaccgcc
    31441 ggggatctgg catagcgtat aaagccaggc gaaggccgaa aatacatagc ccatttccgc
    31501 tttcgtaatg ccgaactctt cctgaatatg ggcggaagcc acggccaagt tagcgcggtc
    31561 aacataacaa atgaccaccg taataaagat catcaccagc gtcagataac gccgacgccc
    31621 cggctttgct gcattaacgg gaatatccat agcgagcttt ctccagattt tgggcatagc
    31681 gaagccgctc accatgccct gtaatttaca gagggttata tttttgtatt gctgttttag
    31741 tgcccgatga ggggcttacg tggcaggaat taccactctg ctacgctgtt atcttcatga
    31801 cgccagagcg gattacgcca gtccggggca tttttactga actcaataac tttagcttcg
    31861 tcgatttcca cacctaagcc cggtttcgtt aacggtttaa agaagccgcc aaccatgctg
    31921 aagtcttctt tgtttttcac aaagtcgagt aactccgcgc ctttgttgta atgaatgccc
    31981 atactttgtt actgaagtac cgcgttatag gaaacaaagt cgatatgcag gcaagccgcc
    32041 agtgcaatcg gtccgaacgg acagtgcggc gcaagggtca catcataggc ttctgccatt
    32101 ccagcaattt tgtagcattc ggtaataccg cctgcatggg agagatccgg ttgcagaatc
    32161 gaaataccgc ctgcctccag cacgcgttta aaatcgaaac gtgagaacat gcgctcaccc
    32221 gccgccagtg gaatatgcgt ttgtgccgcc aatttcgggt agtattcggc ttgttccgcc
    32281 agcaccggct cttcaataaa cagcgggcga tacggctcca gttctttaat cagcactttc
    32341 gccattggcg cgctgacgcg accgtggaaa tcaagaccaa actcaatctg attgccaaaa
    32401 gcttcacgaa tttgtgccac ggtgttgact gccgcatcta ccgcgcggga gttatcaatt
    32461 agccccagtt cttcacaacc gttcagtttg aaggtatcga agccgatttc gcgcagggtt
    32521 ttaatgccgt cgataacatc cgccggacga tcgccgccga cccaactgta ggctttaatt
    32581 ttgtcgcgaa ccaggccgcc catcagttgc cagactggcg cattcagcac tttgcctttg
    32641 atatcccata acgcctggtc gatcccggcg atggcgctca tcaggattgg accgccacga
    32701 taaaatccgg cgcgatacat cacttgccat aagtcgttga tgcgcgaagg atcctgacca
    32761 atcaaatagt cacccagctc gtgaactgcc gcttccaccg tacgggcgcg gccttcaatc
    32821 acgggctcgc cccaaccgac cacgccttca tcggtttcaa ttttcaggaa catccagcgg
    32881 ggaggtaaac gatacgtggt aattttggtg attttcattg cactgcctct cgatacgcct
    32941 taacaaatgc tgctgcctgc tgcgcggtgc gttctacgga ctgcccggcg cgataaagat
    33001 cgctgcctaa gcccgcccca acacaacctg cgtctatcca ctgcgccagg ttttctggcg
    33061 tcacgccgcc aacggcaaag actgcgatgt ccggtggtaa taccgctttt aacgctttga
    33121 tgtattgcgg accaaaagcc gatgacggaa atattttcag cgcctgcgcg cccgcttcga
    33181 gcgcggtaaa ggcttcggtc gccgtcgcgc agccggggca aacggtcatg ccgtagccca
    33241 ccgcgcggcg gatcacttca ctatggatat tgggcgtaac gatgagctga cagcccatcc
    33301 tggcgagcgc atcgacctgt tcaggtttca gtaccgtacc tgcgccaatc aacgccttgt
    33361 cgccatacgc atcaacgatg gcgggaatgc tctgctccca ttgtggggaa ttcagcggga
    33421 tttcaaccgc gtcgaacccg gcgtcaatca ccgcgccaac atgcgccagc gcctcgtcgg
    33481 gcgtaatgcc gcgcaaaatg gcgatcagcg ggagtttagt ttgccactgc atgagcgatg
    33541 ctccttatac cagcctgaaa tgccgtgtcg cccgccaccg ccgccacgtc gcaacccatc
    33601 gcctgaaagg cttgctggta gcgcgcggtc agcgatgttc cggcgacaag ggtgatggcg
    33661 tgttgatggg tcacatagtc gcgcatactg gcaacctctg cgccaatcaa caaaccagag
    33721 agaaattcgc tgacctgttc gcggggaagt gttcccagca catgcgaggc gcgaacttca
    33781 aaaagctgcg gcaatatggc gggcgcatta aggccgcgct caaggccagc cgcgaaggca
    33841 tcggcagagt tttcctgcgg cggcaaacct gcgccaatca atgagtgatt taacagtaaa
    33901 tgatgtaatt caccggtcat cacggtgcga aaatcgttga tttgctggct atcggcctgc
    33961 acccatttgc aatgcgtacc gggcataaca taaagagagg aaggagccag agctcgcgcg
    34021 ccgatcaatt gtgtttcttc gccgcgcatc acattgtggt tatcgtcatg agagacgcat
    34081 aatccgggaa taatccagat attgtcgcca acagacgtta attgttcgcc aatagacgaa
    34141 aaacgggcag gaacagataa atacggagca actttccagc cgacattgct gccgaccatt
    34201 cctgccatca ccactggcgt attttcttca cgccagtcgg tcgtgacttc tgctaacacc
    34261 acagccggag attttccgtt caggcgcgtg acgcctgctt ctgattgcct gctctccagg
    34321 cagtggtcgc cctgataaag ccaggcgcgc agattggtcg atccccagtc aattgcgatg
    34381 tagcgagctg tcatgtgatt tcctttaacc ttcgtgtcga gctggcaatc atggtaagcg
    34441 ccgcctgctc tgccgcatcg ccgtcctgat gccgtatcgc atcgaacagc gccttatgtt
    34501 cctggagcgt ttgcggcatg ttggcctcat cgcccatcca ggttcgttca aaaaccgccc
    34561 gctgcagcga actgatcgca atgctaagtt gctgtaacac cgggttatgc accgactgca
    34621 gcaccgcctc gtggtagcga atatccgctt cgttaaacgc ttcgcggtcc tgattgttgg
    34681 caatcatctc gttcagcgcc gattcaatct gcgccagatc gctggaggtc gcgcgctctg
    34741 ctgcccaacg ggcaatcgcc ggttccacca gatttcgcac ttcgctcatg gcactgataa
    34801 gccgtgggtc gtagtcgttt tccagcaccc attgcagtac gtcagtgtcg aggtaattcc
    34861 actggttacg cggtgccaca aacgccccgc gataacgttt catttcaatc agccgcttcg
    34921 ccatcagcga acggaacacc tcacggatga tgttgcgcga ggttgcaaac tcttcacaga
    34981 gctccgcctc agccggaagt ggcgagccag gtacgtattt gccgtgaacg atctgtttac
    35041 ccagcgtaat gacaatgcga tcggttttat tgagagtcat ggagagtcct tgtgctctgt
    35101 atgttcttct ctactttacc ccgatcgatg cataacgcgg caactttgta gtaccagcgt
    35161 gatgacgttc gcgtttgccg cgcgcgtaat gtagtacaaa cttatattgt tatgctacaa
    35221 tttagatcaa aaaaagaaca atacataaaa aaatgacatg cgtcgggcag aaatctgaaa
    35281 agggatatca ggcgctaaac aggagggaaa gaagagtatg ctttcaacgg cttagctact
    35341 cgtttaaagg attaatcatg aagttgaatt ttaagggatt ttttaaggct gccggtttat
    35401 tcccactggc gctgatgctt tcaggctgta tctcgtatgc tctggtttcc cataccgcaa
    35461 agggtagttc aggaaagtat caatcgcagt cagacaccat cactgggcta tcgcaggcaa
    35521 aagatagtaa tggaacaaaa ggctatgttt ttgtagggga atcgctggat taccttatca
    35581 ctgatggtgc cgatgacatc gttaagatgc tcaatgatcc agcacttaac cggcacaata
    35641 ttcaggttgc cgatgacgca agatttgttt taaatgcggg gaaaaagaaa tttaccggca
    35701 caatatcgct ttactactac tggaataacg aagaagaaaa ggcactggca acgcattatg
    35761 gttttgcctg tggtgttcaa cactgtacca ggtcactgga aaacctaaaa ggcacaatcc
    35821 atgagaaaaa taaaaacatg gattactcaa aggtgatggc gttctaccat ccatttaaag
    35881 tgcgatttta tgaatactat tcacccagag gcattccgga tggtgtttcc gcagcattac
    35941 tgccagtgac tgttacactg gacatcatta ctgcaccgct gcaatttctg gttgtatatg
    36001 cagtaaacca ataatcagta agcgggcaaa cgcgtttatg ctgtttgccc gcttacagat
    36061 taattcagca catacttctc aatagcaaac gccacgccat cttcaaggtt ggattttgtg
    36121 acaaagttcg ccacttcttt caccgaagga atagcgttat ccatcgccac accgacgcct
    36181 gcatattcaa tcattgcgat gtcgttttcc tgatcgccaa tcgccatgat ttcttccggt
    36241 ttaataccta acacgtcggc cagtgatttc acacccgtac ctttgttaac gcgtttatcg
    36301 aggatttcga ggaagtacgg cgcacttttc agcacggtat atttctcttt cacttcctgc
    36361 ggaatacgcg cgatagcctg gtcgaggatg gcgggttcat caatcatcat cactttcagg
    36421 aactgggtat tggggtccat tttctccgct tcgcagaaca ccagcggaat tgtggcaacg
    36481 aaggattcat gcaccgtgta gtagctgata tcgcggttag cggtgtacag cgtggtgcgg
    36541 tccagggcgt ggaaatgaga accgacttcg cgagagagtt tttccaggaa acgatagtcg
    36601 tcatagctga gagcggtttg cgccacggtg ctaccatcag cggccttctg taccagcgcg
    36661 ccgttataag taatgcagta gtcgcccggc tgttccatat gcagctcttt caggtagttg
    36721 tgcacacctg catacggacg acccgtcgtt agcacgacat tcacgccacg ggcgcgagct
    36781 gcggcaatcg catttttaac ggcgggtgaa atggtgtgat cgggcagcag aagggtgcca
    36841 tccatatcga tagcaatgag tttgatagcc atgagttccc aaggtagatt agttcctgac
    36901 ccatgctaac gcgattccgc tcaaaaatca gtacaacacc cgagggaaaa ggggatgcaa
    36961 cgcgggttgc atgctcccca tttgcttagc ggaagagttt ccctttcagc agttccatgc
    37021 ctgcggaaag cagatcgtta ttggcttgtg ctgacacttc accttgcggt gagagcgcat
    37081 caataatctt cggcaattgc tctgccagta aactggaagc cgtactggta tccacgccaa
    37141 gtttttgccc gagatcggac accgcatttg tgccgagcgc cgactccagt tgctcgccgc
    37201 taactgattg atttctctgt tgattactca accaggttga gagaatggcc cctaagccgc
    37261 cactttgcag tttttccagc agcacctgaa tgccaccctg ctcctcaacc caacttaaaa
    37321 tcgcctgata tttccccgca tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc
    37381 ccatgataat cacctgtaaa gcgttacgtg ttgacccaaa aagtatagat ctgcgggatg
    37441 ataattgcgg attgcagaaa taaaaagggc ggagatgatc tccgcccttt tcttatagct
    37501 tcttgccgga tgcggcgtga acgccttatc cggcctacaa aatcatgcaa aatcaatata
    37561 ttgcaggagt ctcgtaggac taataagcgt agcgcatcag gcacgctcgc atggttaacg
    37621 ccattaaata tcaatattcg ccgctttcag ggcgttctct tcaataaacg cacgacgcgg
    37681 ttctacggcg tcgcccatca gcgtggtgaa caactggtca gcggcaatcg catccttaac
    37741 ggtaacgcgc agcatacgac ggctttccgg gtccatggtg gtttcccaca gctgttccgg
    37801 gttcatctcg cccagacctt tataacgctg gatggagagg ccacgacggg actctttcac
    37861 cagccagtcc agcgcctgct cgaagctggc taccggctga cgacgctcgc cacgttcgat
    37921 aaacgcatct tcttccagca agccacgcag tttctcaccc agcgtgcaga tacgacgata
    37981 ttcgccaccg gtgataaact cgtgatccag cggatagtca gtatccacac cgtgggtacg
    38041 cacgcgaaca atcggctcga acaggttttg ttcggcattg gtgtggacat caaacttcca
    38101 ctggctgccg tgctgttctt tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt
    38161 aacggtctgc tcatcagaaa ggtcagcttc cgtcaacgtc ggctgataga taagctcttt
    38221 cagcattgct ttcggataac gacgctccat gcggttgatc attttctgcg tcgcgttgta
    38281 ctcagatacc agtttctcta acgcttcgcc agccaatgcc ggtgcactgg cgttggtgtg
    38341 cagcgttgcg ccgtccagcg cgatagagat ctggtactga tccatcgctt cgtcgtcttt
    38401 aatgtactgt tcctgcttgc ctttcttcac tttgtacagc ggcggctgag cgatgtagac
    38461 gtgaccgcgt tcaacgattt ccggcatctg acgatagaag aaggtcaaca gcagcgtacg
    38521 aatgtgcgag ccgtcgacgt ccgcatcggt catgatgatg atgctgtgat aacgcagttt
    38581 gtccgggttg tactcgtcac gaccgatacc acagccaagc gcggtgataa gcgtcgccac
    38641 ttcctgagaa gagagcatct tatcgaagcg cgctttctcg acgttaagga ttttaccctt
    38701 cagcggcaga atcgcctggt tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga
    38761 gtccccttcc accaggtaca gttcggaaag cgccggatcg cgttcctggc agtctgccag
    38821 tttgcccggc aggccagcta agtcgagcgc acctttacgg cgggtcattt cacgcgcgcg
    38881 acgcgccgct tcacgggcac gggcagcatc gataattttg ccaaccacga ttttcgcatc
    38941 agttgggttt tccagcaggt attccgccag cagttcgttc atctgctgtt caaccgccga
    39001 tttcacctca gaagaaacca gtttgtcttt ggtctgggag gagaatttcg ggtccggcac
    39061 ttttacggaa acgaccgcaa tcaggccttc acgcgcatcg tcaccggtgg cgctgacttt
    39121 ggcttttttg ctgtagcctt ctttgtccat gtaggcattc agggtacggg tcatcgccgc
    39181 acggaagcct gccaggtgag taccgccgtc acgctgcgga atgttgttgg taaagcagta
    39241 gatgttttcc tggaagccat cgttccactg cagcgccact tcaacgccaa taccgtcttt
    39301 ttcggtggag aagtagaaga tatttgggtg gatcggcgtt ttgttcttgt tcagatattc
    39361 aacgaacgct ttgatgccgc cttcatagtg gaagtggtct tctttgccgt cacgcttgtc
    39421 gcgcagacga atggaaacgc cggagttgag gaacgacaac tcacgcagac gtttcgccag
    39481 aatttcatat tcgaactcgg tcacattggt gaaggtttcg aggctgggcc agaaacgcac
    39541 catggtgccg gttttttcag tctcgccggt aaccgccagc ggagcctgcg gtacaccgtg
    39601 ttcgtagatc tgacggtgaa ttttaccctc gcgctgaata accagctcca gtttttgcga
    39661 cagggcgttt actaccgaaa caccaacgcc gtgcagaccg ccggacactt tataggagtt
    39721 atcgtcaaat ttaccgcctg cgtgcagaac ggtcatgatc acttccgccg ccgatacgcc
    39781 ctcttccggg tgaataccgg tcggaatgcc gcgcccgtca tcctgtacag agacagagtt
    39841 atcggcgtga atggtgacga taatttcttt acagtgaccc gcgagcgctt cgtcgatagc
    39901 gttatctacc acctcgaata ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat
    39961 atacataccc gggcgcttac gcaccgcatc cagccctttc aggactttga tactggagga
    40021 gtcataagaa ttcgacatca acgtttctcg ctcatttata cttgggttaa tctgttattt
    40081 tacccttttc cacggtaaac atcttcgaat tttcgtccga catgtctata acgtgttcag
    40141 cactgatcgc gctgacaaag acctgtgatt gcgtcgcttt taagcggctg gcaagcaacc
    40201 cgcgacgctc atcatcaagc tcagaggcaa aatcatctat caggtagaga caccgccgcc
    40261 cgctttcacg agtgaggaac tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa
    40321 gctgcccacg cgataaggta tcttccaccg gcgcaccgtc ggcgcgaatg cgtaagtccg
    40381 ctttatgcgg gccatgcgcg gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca
    40441 gcacctcagc gtactctgtc tctttctccc agccgcgctg gaaagagaaa gtcagagaaa
    40501 actcagggag aaattgctta caggtatcat ccatatcggc cgcgataccg gcgctatact
    40561 ccgcgcgcca ggtgctgatt tgctccgcca gcgggatcag ttctttatcc cacgggcgta
    40621 gctgttcgta acgagtcacc tggcgcagcg ccgcattgcg ctgcttgagc aatcgcttga
    40681 gattgctcca ggcggtgaaa aatccaggtt cgttgtgaaa gcatccccag tcgaggaatg
    40741 ctcttctgta tttggggccg ccgttgagta aagtaaaccc ttctggcgtt atcaactgca
    40801 tcggcatcag gtgcgccagt tccgcgactt tatgcccgtc cgtaccgtca atacggactt
    40861 tgctgtcgcc ctgtttgtct ttggttaagc caatcgccgt ctcgcgctct tcgccctgta
    40921 atcgcccgtg gagaacaaat gcctcctgct catggcgaat gacgcgacca atctgcaaac
    40981 tgcgaaacgc ccgaccatgg ccgagcgtat agatggcttc cagcacgctg gttttgccac
    41041 tgccgttggc acctaccaga aagttaaagc cgggagataa agcgagatcc gcggtttcaa
    41101 tattgcggaa atcgcggatc aacaagcggg tgagggacat tacagtctca ttggcatgac
    41161 aacataggca gccgactgtg atgcggcatc ttcaatctgc acgctggaaa ccgaatcggt
    41221 cagcatcatg cggacgtttt cgcatttcag cgcgttcaga acatccagca catagctgac
    41281 gttgaagccg atttccatct ccgcaccgct ataggtaacg tcgaggatct cttccgcttc
    41341 ttcctgttcc gggttgttgg cagtaatttt cagctggttt tcgctgacat acaggcgcac
    41401 accgcggaat ttctcgttag agagaatcgc cgcgcgagca aacgcctgct tgagcagatc
    41461 gcagcctgct tccagatgtt tgtccgggtt cttcggcaga acgcgacggt aatccgggaa
    41521 gcgaccatcc accagtttcg aggtgaaaat aaagtcgcca acgtgggcac gaatgttatt
    41581 gctgccaatc tgcacacgca gcagattgtc gccgccgtcg agcatacgca tcagttcaat
    41641 cacgccttta cgcggtacga tcaccgaatg gcttggcaaa gattgaccaa ttggcattga
    41701 acagaccgcc agacggtggc cgtcggttgc cacggtgcgc agttcttcac cttcggtttc
    41761 aaacagcata ccgtttaagt aatagcgaac atcctgatgc gccatcgaaa actgggttgc
    41821 ttcaatcaga cgcttcatcg tcgcctgcgg cagggtaaat tcgacttcgc tctgccagtc
    41881 atcgaggttc gggaaatccg ccgctggcag ggtagacagc gaaaaacggc tacgcccgga
    41941 gcgcaccagc atccgttcac cttccagctg cacggcaatt tccgcgcctt caggcagacc
    42001 acggcagata tcaaagaatt tgcgcgccgg aacggtcgtc gctcctggct cgtgtggctg
    42061 aaccagcgca acgcgtgcca ccatttccat ctcgagatca gtaccggtca gcgacaacgt
    42121 accgtcagca acctgtaaca gcagattacc gagaatcggt agcgtaggac gaccacctaa
    42181 cggaccgctc acctgttgta gcggttttaa taaatgctca cgttctacgg taaatttcat
    42241 aggtttacga tgacaatgtt ctgattaaat ttgaaaaatc ttctttgata tcgtggctct
    42301 cttcacgcaa ctgctcgatc ttacggcagg catgaagcac cgtcgtgtgg tcacgcccac
    42361 caaacgcatc gccaatctct ggcagactgt ggttagtcag ctctttcgct agcgccatcg
    42421 ccatctggcg cggacgcgcc accgagcggg atcgacgctt ggaaaggaga tccgcgactt
    42481 tgatcttgta gtactccgcc accgtcttct gaatattgtc gatggtgacc agtttttcct
    42541 gcaatgccag caagtcgcgc agcgcctcac gcacgaagtc gatggtgatc gcccgtccgg
    42601 taaagttggc gttagcaata acgcggttca gcgccccttc cagctcacgt acgttagatc
    42661 gtagacgctt ggcgataaag aacgccactt cacccggcaa acgaatgtca ttttcgtcgg
    42721 cctttttcat caggatcgcc acacgggttt ccagctctgg cggttcgatc gccacagtca
    42781 gtccccaacc gaagcgggat ttcaaacgat cctcaacgcc gttgatctct ttcggatagc
    42841 gatccgaggt gagaatgatc tgttgattac cttccagcag ggcgttgaag gtgtggaaaa
    42901 actcttcctg agatcgttct ttattagcaa aaaactgaat atcgtcgatc agcagtgcat
    42961 ctacggaacg gtagtagcgt ttaaactctt cgatcgcgtt gttttgcagg gctttaacca
    43021 tgtcctgaac aaagcgctcg gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca
    43081 taatgccgtt acccaccgca tgcagcaggt gagttttacc cagacccgtg ccgccataaa
    43141 ggaacaacgg gttataggca ccgccagggt tatccgccac ctggcaagcc gccgcgcgcg
    43201 ccagttggtt agatttacct tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag
    43261 aacgataggt cggttctgcc ggggccggga cgttatccca acctgagcgc gtagaaggcg
    43321 cagcacgttg cggctgcgtt tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg
    43381 ccgcttgtgg cgtttgcgtc accggtttgg tgccgacttc aaaacgcagc tgtggggcat
    43441 ccgctccgca gaaactggtt agcagtccat tgatattatt aaggtacttg tcccgtaccc
    43501 aatcgaggac aaaacggttt ggcgcgtaca gggccagcgt gttatcgctc agttccgcct
    43561 gcaatgggcg tatccacata ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa
    43621 gacactgctg ccaaagcgaa agtgacacgg cggactccac tcgaacaaaa gtcgataatg
    43681 actaaggctg aaacattcat gattgttgac gtacgtcgaa aagaccctgc ttgagggtga
    43741 cgcacgaacc gctgtctgcg gttatatgcc cgatcaagat cctgcaaaac gatcgggacc
    43801 gcggatcata gcctaaactg cgcaagagat cttctgtttc tcacagattt tccctattta
    43861 tccacaggac tttccagaac tcgctaagtg taaacgatcc tgccgcgagg cgggcacgat
    43921 ttacgccgca tattggaaaa tttaatgacc atagacaaaa attggcttaa tcgatctaat
    43981 aaagatccag gacgatcctt gcgctttacc catcagcccg tataatcctc cacccggcgc
    44041 gccatgctgg tttccactgg tgtgaggtcg tacattttcc ctgcgaaaag gtgcggaaaa
    44101 gcgcggtaaa taaggaaaga gaattgactc cggtgtgtac aattattaca atccggcctc
    44161 tttaatcacc catggcttcg gtgtccatcg tttcattttt cggcggatat ccaataaagc
    44221 cattgaattt attcaagttt aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt
    44281 actgaagcgc aaccgttctc acggcttccg tgctcgtatg gctactaaaa atggtcgtca
    44341 ggttctggca cgtcgtcgtg ctaaaggccg cgctcgtctg accgtttcta agtaataaag
    44401 ctaacccctg agtggttaag ctcgcatttc ccagggagtt acgcttgtta actcccagtc
    44461 aattcacatt cgtcttccag cagccacaac gggctggcac gccgcaaatt accattctcg
    44521 gccgcctgaa ttcgctgggg catccccgta tcggtcttac agtcgccaag aaaaacgttc
    44581 gacgcgccca tgaacgcaat cggattaaac gcctgacgcg tgaaagcttc cgtctgcgcc
    44641 aacatgaact cccggctatg gatttcgtgg tggtggcgaa aaaaggggtt gccgacctcg
    44701 ataaccgtgc tctctcggaa gcgttggaaa aattatggcg ccgccactgt cgcctggctc
    44761 gcgggtcctg atagccctca ttcgggtcta tcaacgcctg attagtccgc tactcgggcc
    44821 gcattgtcgt ttcactccaa cctgttcaag ctacggaatt gaggcattgc gcaggtttgg
    44881 agtgataaaa ggcagttggt tgacggtgaa acgcgtatta aaatgccacc ctttacaccc
    44941 tggtggtgac gatcccgtcc cgcccggacc atttgatacc agagaacact aacgatggat
    45001 tcgcaacgca atcttttagt catcgctttg ctgttcgtgt ctttcatgat ctggcaagcc
    45061 tgggagcagg ataaaaaccc gcaacctcag gcccaacaga ccacgcagac aacgaccacc
    45121 gcagcgggta gcgccgccga ccagggcgta ccggccagtg gccaggggaa actgatctcg
    45181 gttaagaccg acgtgcttga tctgaccatc aacacccgtg gtggtgatgt tgagcaagct
    45241 ctgctgcctg cttacccgaa agagctgaac tctacccagc cgttccagct gttggaaact
    45301 tcaccgcagt ttatttatca ggcacagagc ggtctgaccg gtcgtgatgg cccggataac
    45361 ccggctaacg gcccgcgtcc gctgtataac gttgaaaaag acgcttatgt gctggctgaa
    45421 ggtcaaaacg aactgcaggt gccgatgacg tataccgacg cggcaggcaa cacgtttacc
    45481 aaaacgtttg tcctgaaacg tggtgattac gctgtcaacg tcaactacaa cgtgcagaac
    45541 gctggcgaga aaccgctgga aatctcctcg tttggtcagt tgaagcaatc catcactctg
    45601 ccaccgcatc tcgataccgg aagcagcaac ttcgcactgc acaccttccg tggcgcggcg
    45661 tactccacgc ctgacgagaa gtatgagaaa tacaagttcg ataccattgc cgataacgaa
    45721 aacctgaaca tctcttcgaa aggtggttgg gtggcgatgc tgcaacagta tttcgcgacg
    45781 gcgtggatcc cgcataacga cggtaccaac aacttctata ccgctaatct gggtaacggc
    45841 atcgtcgcta tcggctataa atctcagccg gtactggttc agcctggtca gactggcgcg
    45901 atgaacagca ccctgtgggt tggcccggaa atccaggaca aaatggcagc tgttgctccg
    45961 cacctggatc tgaccgttga ttacggttgg ttgtggttca tctctcagcc gctgttcaaa
    46021 ctgctgaaat ggatccatag ctttgtgggt aactggggct tctccattat catcatcacc
    46081 tttatcgttc gtggcatcat gtacccgctg accaaagcgc agtacacctc catggcgaag
    46141 atgcgtatgc tgcagccgaa gattcaggca atgcgtgagc gtctgggcga tgacaaacag
    46201 cgtatcagtc aggaaatgat ggcgctgtac aaagctgaga aggttaaccc gctgggcggc
    46261 tgcttcccgc tgctgatcca gatgccaatc ttcctggcgt tgtactacat gctgatgggt
    46321 tccgttgaac tgcgtcaggc accgtttgca ctgtggatcc acgacctgtc ggcacaggac
    46381 ccgtactaca tcctgccgat cctgatgggc gtaacgatgt tcttcattca gaagatgtcg
    46441 ccgaccactg tgaccgaccc gatgcagcag aagatcatga cctttatgcc ggtcatcttc
    46501 accgtgttct tcctgtggtt cccgtcaggt ctggtactgt actatatcgt cagcaacctg
    46561 gtaaccatta ttcagcagca gctgatttac cgtggtctgg aaaaacgtgg cctgcatagc
    46621 cgcgagaaga aaaaatcctg attcggtgag ttttcgctaa aataagggcg gtcagttgac
    46681 cgcctttttt cttttcgtag ggcggataag caccgcgcat ccgccacaca aagcaacagg
    46741 aacatcatga gcgataatga cactatcgta gcccaggcca cgcctccggg acgtggcggc
    46801 gttggcatcc tgcgcatctc cggcttcaaa gcccgtgaag ttgccgaaac cgtgctgggt
    46861 aaactgccta agccgcgcta cgccgattat cttccgttta aagacgccga cggcagcgtg
    46921 ctcgatcagg ggattgcgct atggttccct ggcccgaact cgttcaccgg cgaagatgtg
    46981 ctggaactgc aaggtcatgg cggtccggtg atcctcgacc tgctgttaaa acgcattctg
    47041 accattcccg gcctgcggat tgctcgccct ggtgagtttt ccgaacgcgc gtttcttaac
    47101 gataaacttg acttagccca ggccgaggcg attgccgatc ttatcgacgc cagttcggaa
    47161 caggcggccc gttcggcact taactcgctg caaggcgcat tctccgcacg ggttaatcat
    47221 ctggtagaag ccctcaccca cttgcgcatt tacgtcgaag cggcaattga tttccctgat
    47281 gaagagatcg atttcctctc cgacggcaaa attgaagccc agcttaatga cgtgatggcc
    47341 gatctcgatg cagtgcgtgc tgaagcacgt cagggtagtt tgttgcgcga agggatgaaa
    47401 gtagtgattg ccggacgtcc taatgccggt aaatcgagcc tgttaaacgc gctggcgggc
    47461 cgtgaagcgg caatcgtaac cgatatcgcc ggaaccacgc gtgacgtgct gcgtgagcat
    47521 atccacattg acggaatgcc gctgcatatc atcgacactg ccgggctacg tgaggccagt
    47581 gacgaagtgg aacgtattgg tatcgagcgc gcgtggcagg aaattgaaca ggccgaccgc
    47641 gtgctgttta tggtcgatgg caccacgaca gacgccgttg atccggcaga gatctggccg
    47701 gaatttatcg cccgtctgcc cgcgaaactg ccgatcaccg tggtacgcaa taaagccgat
    47761 atcaccggcg aaacgctggg aatgagtgaa gtgaacggtc acgcgttaat tcgtctctcg
    47821 gcaaggactg gtgaaggcgt ggacgtgctg cgtaaccatc tcaaacagag catgggcttt
    47881 gacaccaata tggaaggcgg cttcctggcg cgccgtcgcc acttacaggc gctggaacag
    47941 gcagcggagc atttgcagca aggtaaagcg caactgttgg gagcctgggc aggtgaactg
    48001 ctggcggaag agttgcgcct ggcacagcag aacttaagcg aaatcaccgg ggaatttact
    48061 tcagacgacc tgctggggcg gattttctcc agcttctgta ttggtaagta accgcgctta
    48121 cgaagccgta ttctgactgt cagatgcggc ttcgcttcat tgttagcact cctgttatgc
    48181 ctcaaccctt ttttaaacat taaaattctt acgtaattta taatctttaa aaaaagcatt
    48241 taatactgct ccccgaacga ttgtgattcg attcacattt aaacaatttc agaatagaca
    48301 aaaa
//
LOCUS       DAECNO010000018        47955 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.018, whole genome shotgun sequence.
ACCESSION   DAECNO010000018 DAECNO010000000
VERSION     DAECNO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.018"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="KKO08_001048"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="KKO08_001048"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBD6317384.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="KKO08_001049"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="KKO08_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBD6317385.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KKO08_001050"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="KKO08_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBD6317386.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KKO08_001051"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="KKO08_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBD6317387.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KKO08_001052"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="KKO08_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBD6317388.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="KKO08_001053"
     CDS             5377..5835
                     /locus_tag="KKO08_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBD6317389.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="KKO08_001054"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="KKO08_001054"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBD6317390.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="KKO08_001055"
     tRNA            6887..6971
                     /locus_tag="KKO08_001055"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="KKO08_001056"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="KKO08_001056"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KKO08_001057"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="KKO08_001057"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBD6317391.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KKO08_001058"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="KKO08_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBD6317392.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KKO08_001059"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="KKO08_001059"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBD6317393.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="KKO08_001060"
     CDS             complement(10252..13995)
                     /locus_tag="KKO08_001060"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBD6317394.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="KKO08_001061"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="KKO08_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBD6317395.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="KKO08_001062"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="KKO08_001062"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBD6317396.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="KKO08_001063"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="KKO08_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBD6317397.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="KKO08_001064"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="KKO08_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBD6317398.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="KKO08_001065"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="KKO08_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBD6317399.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="KKO08_001066"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="KKO08_001066"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBD6317400.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="KKO08_001067"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="KKO08_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBD6317401.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="KKO08_001068"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="KKO08_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBD6317402.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="KKO08_001069"
     CDS             23392..23499
                     /locus_tag="KKO08_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBD6317403.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="KKO08_001070"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="KKO08_001070"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBD6317404.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="KKO08_001071"
     CDS             complement(24538..25347)
                     /locus_tag="KKO08_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBD6317405.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="KKO08_001072"
     CDS             complement(25351..25743)
                     /locus_tag="KKO08_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBD6317406.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="KKO08_001073"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="KKO08_001073"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBD6317407.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="KKO08_001074"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="KKO08_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBD6317408.1"
                     /translation="MKPSIVAKLEALHERHEEVQTLLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="KKO08_001075"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="KKO08_001075"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBD6317409.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="KKO08_001076"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="KKO08_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBD6317410.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="KKO08_001077"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="KKO08_001077"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBD6317411.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="KKO08_001078"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="KKO08_001078"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBD6317412.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="KKO08_001079"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="KKO08_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBD6317413.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="KKO08_001080"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="KKO08_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBD6317414.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="KKO08_001081"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="KKO08_001081"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBD6317415.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="KKO08_001082"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="KKO08_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBD6317416.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36789..39656
                     /locus_tag="KKO08_001083"
     CDS             36789..39656
                     /locus_tag="KKO08_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD6317417.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="KKO08_001084"
     CDS             complement(39756..41675)
                     /gene="dhaR"
                     /locus_tag="KKO08_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBD6317418.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41903..42973
                     /gene="dhaK"
                     /locus_tag="KKO08_001085"
     CDS             41903..42973
                     /gene="dhaK"
                     /locus_tag="KKO08_001085"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBD6317419.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42984..43616
                     /gene="dhaL"
                     /locus_tag="KKO08_001086"
     CDS             42984..43616
                     /gene="dhaL"
                     /locus_tag="KKO08_001086"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBD6317420.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43627..45045
                     /gene="dhaM"
                     /locus_tag="KKO08_001087"
     CDS             43627..45045
                     /gene="dhaM"
                     /locus_tag="KKO08_001087"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBD6317421.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45365..47062
                     /gene="treA"
                     /locus_tag="KKO08_001088"
     CDS             45365..47062
                     /gene="treA"
                     /locus_tag="KKO08_001088"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBD6317422.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47141..47580)
                     /locus_tag="KKO08_001089"
                     /pseudo
     CDS             complement(47141..47580)
                     /locus_tag="KKO08_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12035 a        12339 c        11748 g        11833 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgct ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggca cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcta
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccac ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccaa gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aagcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgtctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ttggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta cgacaactta gggaataact cgatataaac tcacctttgt
    36601 aagggggggg ttaaatatat attaattcct gtgttcctga ttttgcccgg taatagagaa
    36661 ttattctcag agaagctcat catgattgca ccatacagat atcatgatga taagaggata
    36721 ttattcattg acttaaacaa aatgacaaaa tccatagacc catagcctgg gtactatata
    36781 ggagtggtat gggcatcaaa caacacaatg ggaataccaa agccgatcgt ctcgctgaat
    36841 taaaaatccg ttcgccctca attcaactga taaaatttgg cgctattggt ttgaatgcaa
    36901 ttatcttttc ccccctgctg atagctgctg atacaggaag tcaatatggc accaatatta
    36961 ctattaatga tggtgacaga attacaggag ataccgccga tccatcagga aacctctatg
    37021 gtgtaatgac cccagcagga aacacgcctg gcaatatcaa cctgggtaat gatgtcaccg
    37081 tcaatgtcaa cgacgcctct ggatatgcaa aaggaatcat tattcagggc aaaaacagct
    37141 ccctgacagc taaccgactc acagtagatg ttgttggtca aacctctgcc atcggcatta
    37201 acttaattgg tgactatacc catgctgact taggcacagg cagcaccatt aagagtaacg
    37261 atgacggcat cattattggg catagctcaa cactaacagc cactcaattc accattgaaa
    37321 actcgaacgg tataggccta accatcaatg actatggcac cagtgtcgat cttggaagcg
    37381 gaagtaaaat caagaccgat ggaagtacag gtgtttatat cggtggtctc aacggcaata
    37441 acgccaatgg tgctgcgcgt tttacggcga cagacctgac aatcgatgtt cagggctaca
    37501 gcgccatggg gataaacgta cagaaaaact ctgttgtcga tctcggaaca aacagtacca
    37561 ttaaaaccaa tggcgataat gctcacggcc tctggagctt tggccaggtt agcgcgaatg
    37621 cactcactgt tgatgtaact ggagccgcgg ccaatggcgt cgaagttcgt ggtggtacaa
    37681 ccactatcgg tgcagatagc catatttctt ccgcgcaggg cggtggcctc gtcaccagta
    37741 gttcagacgc gacaatcaat ttttctggca cggcagcgca acgaaacagc atcttttccg
    37801 gcggttctta tggtgcctcg gcccagacgg caacggctgt tatcaacatg caaaataccg
    37861 atattacggt tgatcgtaat ggcagtctgg cgctgggttt gtgggcgctc agcggcggta
    37921 gaataaccgg agacagtttg gctatcaccg gcgcggcagg agccagaggg atttatgcca
    37981 tgaccaacag ccagatcgac ctcacgagcg atctggtcat tgatatgagt acacccgacc
    38041 agatggccat cgcaacgcaa catgacgatg gttatgccgc cagccgcatc aacgcctcgg
    38101 gtcgtatgct tatcaacggt agcgttcttt ccaaaggtgg gctaatcaat ctggatatgc
    38161 accctgggtc ggtttggaca ggttcctccc tcagcgataa tgtcaatggc gggaaactgg
    38221 acgttgcaat gaataacagc gtctggaacg taacaagtaa ttctaatctc gacacgctgg
    38281 cgctgagcca ttcaactgtc gattttgcca gccacgggtc aactgccggc acatttacca
    38341 cattaaacgt agagaacctg agcggtaaca gtacctttat tatgcgtgct gatgttgttg
    38401 gcgagggtaa tggcgttaat aatagagggg atttattgaa tatcagcggg agtagtgctg
    38461 gtaatcacgt attggctatc cgcaaccagg gcagcgaggc cacaacggga aatgaagttc
    38521 tgacagtggt aaaaaccact gacggcgcgg cctcgttcag cgcgtcttct caggttgagt
    38581 tggggggata tctgtacgat gtgcgtaaaa atggcactaa ctgggagctt tacgcttccg
    38641 ggacagttcc ggaaccgact cctaatcctg aacccacacc agctcccgct cagcctccca
    38701 tagtcaaccc cgatcctacg cctgaacccg ctcccacgcc taaacccacc acgaccgcag
    38761 atgctggcgg caattatctc aatgtcggtt acttattgaa ctatgttgaa aaccgtacgc
    38821 tgatgcaacg gatgggtgac ttgcgaaatc agagtaaaga cggtaatatc tggttgcgca
    38881 gttatggggg aagcttggac tcctttgcca gtggcaaact gagcggcttt gacatgggtt
    38941 acagcggtat ccagtttggt ggggataaac gtctctctga tgtaatgccg ttgtatgtcg
    39001 gtctgtatat tgactcaaca catgcatcgc cggactatag cggaggcgac ggtaccgcac
    39061 gttcagacta catgggaatg tacgccagtt acatggcaca aaacggtttt tacagcgatc
    39121 tcgttataaa agcatcgcgc cagaaaaata gtttccacgt actggacagt cagaacaacg
    39181 gcgttaacgc caacggcact gcgaatggaa tgagcatctc cctggaagcc gggcagaggt
    39241 tcaacctgtc ccctactggt tatgggttct atatagagcc gcaaacccag cttacataca
    39301 gccaccagaa tgagatggct atgaaggcga gtaatggcct caatatacat ctgaatcact
    39361 acgaatcgct gctggggcgt gccagcatga tactggggta tgacatcacc gcaggcaaca
    39421 gccagctgaa tgtctatgtg aagactggcg ctatccgcga gttttcaggg gataccgaat
    39481 atctgttgaa cgactcccgg gagaagtaca gtttcaaagg taatggctgg aataacggcg
    39541 tgggagtcag tgcacagtat aacaaacagc acacattcta tctcgaagcg gattacacgc
    39601 agggtaacct ctttgatcag aagcaagtca acggaggata tcgcttcagc ttttaagtgc
    39661 gggatagaca agcccttaaa cgatgctgaa aattgttagc gtgcgccgga atgaaataca
    39721 gatcactctg atgtatcaga aatcgtttct gtctttcata cccggcgctt aaactgccct
    39781 gcatcaatgc catgttgctt cattttccgc cacagcgtag tgcggccgat cccaagtaaa
    39841 gccgacattt cctgaatgcg accgcctgtg acctgggctg cgttaataat tgcctctttt
    39901 tcaacttccg caaatgacag actggtggaa aggcgggtgg cgctgacatc atctgtcgcc
    39961 tgctcggtaa acagatgttc aggtaaatca ctgacgcgaa tgcgcccgtt atcactactc
    40021 agagcaagat tctcgatgac gctgtaaagt tcaaaatcgt tgcctggcca tgcacaagaa
    40081 actaggcgag cgagggcatc gtcatcaatt ttcagccgcg tagagaagcg tttttcaaga
    40141 ctgcgtaatt tgttattcac cagcgccgga atgctgccac gccgcatacg cagaggcggg
    40201 atggtaattt caaatgcatg cagcgcgtaa tacagctggc gactaaaacg attttgttcc
    40261 accagcattg cgaggtccac ggtcgttgtt gcaatcactt tgacatcaat tggtattaaa
    40321 cgccgcgcat ccagtcgcgt gataaccccc tgcttgataa cctgcagcaa agcagactgt
    40381 aactccaccg ccagatattc aatcttttca agaaacagcg tgccgccgtg tgccagttcc
    40441 agccgactca gacggccatt ttcattgtcc gtgcgatcgc caccaataaa ttcttccgcc
    40501 agcgcagcat caccatataa ctcacaattg acggcgatat aaggacctgc agcacgctcg
    40561 ctttcattat gaattgcctg acttagcagt gccttgccca cgccctcttc tccacaaagc
    40621 aggacaggaa agctactgcg cgccgcctgg cgaccaaaat gaatcaagcg gcgggtttgc
    40681 ggatcgtcct gtggcatatg agcgaaggta tggctgactt ttcctaattg actggtcatt
    40741 aactgccgca tctgttccac aggatggagc aacaaaataa agctggttcc ctgcgtttct
    40801 attatcggtt taagggttat caccgcatca ataaactggt gctggctttc aaaggttgct
    40861 tctacgtgtt tgagcggatg tgcctgtttt attgcttgtt gcaatacggc gggtaacgtt
    40921 aagagttcag tgattgcccg tccctgactt gccgtcgcgt caaggcgcaa gacccgcgcc
    40981 gcctgggcat taataaattg caaattaccc tgctcgtccc agctaatcac gccatcatcc
    41041 atactttcta acagggcatt aagttgattt aaatgacggt tagtttcagc gagcaaactg
    41101 tccgtcagca gtaaattacc gacctcgcgg gcgattgcca acgtcaacgg caaatcagct
    41161 gcggtagttt gctcaaccgg acacgccagc gctattgttc ccgtcaatcg gcccttgctg
    41221 tcaaacaacg gcgttgcaca aaaggcccag ttccagagtg cctgtttgaa atgttgatcg
    41281 gccatcgttt tcacggcctg accagagata gccgctaacg atagcgcaca agtaccaata
    41341 attccctcgg cgcaatacgt gccgtcattg aatcccagtg cacttagctg ctgcaaggtt
    41401 tgcggatcgc cattacggct gagaatgcag gcggtttcat cgaggataaa cagcgcacac
    41461 tctcgcggtg ccatatattc ccaggcatct tccagcgcag cctgcccgag cgtcagcatc
    41521 gctttcttac gccgataaat agaagcaaat gtcacgccct gggcctgatg tggtacgttc
    41581 catgtctccc gtttcatcag cttattgcat cgctcccagg aggttgcaat taagggagat
    41641 atgccccgac catcgttgtt aaaagcgcca ctcatatccc gcatccctta tgacaaaagg
    41701 tgttccggct cgcaatttta agtattaata cacgtaaaag tttcaatatg gaacattcat
    41761 taaagcatat gtttcactat gaaacataat ttgggctaat tttcttttcc cttgcctgat
    41821 gcacaatgga ttcatcggca gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta
    41881 ccgtaattgc tggagcaaaa taatgaaaaa attgatcaat gatgtgcaag acgtactgga
    41941 cgaacaactg gcaggactgg cgaaagcgca tccatcgctg acactgcatc aggatccggt
    42001 gtatgtcacc cgagctgatg cccctgttgc aggaaaagtc gccctgctgt cgggtggcgg
    42061 cagcggacac gagccgatgc actgtggtta tatcggtcag gggatgcttt cgggggcctg
    42121 tccgggcgaa attttcacct caccgacgcc cgataaaatc tttgaatgct ccatgcaaat
    42181 tgatggcggc gaaggtgtac tgttgattat caaaaattac accggcgata ttcttaactt
    42241 tgaaacagcg accgagttac tgcacgatag cggcgtaaaa gtgaccactg tggtcattga
    42301 tgacgacgtt gcggtaaaag acagtcttta taccgccggg cgacgcggcg ttgccaacac
    42361 agtattaatt gaaaaactcg taggcgcagc ggcggagcgt ggcgactcac tggaggcctg
    42421 tgcggaactg gggcgtaagt tgaataatca aggccactca ataggtatcg ctctcggtgc
    42481 ctgtaccgtt cctgccgcgg gcaaaccttc ttttaccctt gcggataatg agatggagtt
    42541 tggcgtcggc attcatggtg agccaggtat tgaccgccgc cctttctctt cccttgatca
    42601 aaccgtcgat gaaatgttcg acaccctgct ggaacatggc tcataccatc gcactttgcg
    42661 tttctgggat tatcaacaag gcagctggca ggaagaacca caaaccaaac aaccgctcca
    42721 gtctggcgat cgggtgattg cactggttaa caatcttggc gcaactccgc tttctgagct
    42781 gtacggcgtc tataaccgcc tgaccacacg ttgccagcaa gcgggattga ctatcgaacg
    42841 taatttaatt ggcgcgtact gcacctcact ggatatgacc ggtttctcaa tcaccttact
    42901 gaaagttgat gacgaaacgc tggctctctg ggacgccccg gtccacaccc cggcccttaa
    42961 ctggggtaaa taaggagaaa gcaatgtcac tgagcagaac tcaaattgtt aactggctca
    43021 ctcgttgtgg cgatattttc aacaccgaga gcgagtatct taccgggctg gatcgcgaga
    43081 ttggcgacgc tgaccacggg ctaaatatga accgaggctt tagcaaagtg gtggaaaaac
    43141 tccctgctat cgcagataaa gatatcggtt tcattctcaa aaataccggt atgacgctgc
    43201 tttccagcgt cggtggtgcc agtggtccgc tgttcggtac cttctttatc cgcgccgcac
    43261 aggcgaccca ggcacggcaa agcctgacac tggaagagct ttatcagatg ttccgcgatg
    43321 gcgcggacgg cgtaatcagt cgcgggaaag ccgaacctgg cgataaaacc atgtgtgatg
    43381 tgtgggtgcc ggtggtggaa tcgttacgtc agtccagcga gcaaaatctc tctgttccgg
    43441 tggcgctcga agctgccagt agcatcgccg aatccgctgc acaaagtacg attacgatgc
    43501 aagcccgcaa aggccgcgcc agttatctcg gtgaacgcag tattggtcac caggatcccg
    43561 gcgcgacctc ggtgatgttt atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa
    43621 ttggtgatgg taaacctggt catagtttca catagcagcc gactgggaga aggtgtcggt
    43681 gaattagccc gtcagatgtt aatgagtgat agttgtaaaa tcgccattgc cgcgggaatt
    43741 gacgatccac aaaatcccat tggtaccgat gccgtcaaag tgatggaggc catcgaatct
    43801 gttgctgatg ccgaccatgt gctggtcatg atggatatgg gtagcgcatt attgagtgct
    43861 gaaactgcgc tggaattgct ggctcccgag atcgccgcaa aagtacgttt gtgtgctgcg
    43921 ccgttggtcg aaggtacact ggcagcaacg gtcagcgcgg cctcgggggc ggatatcgac
    43981 aaagttatct ttgacgctat gcatgcgctg gaagccaaac gtgaacaact gggtttaccg
    44041 tcctccgaca ctgaaatctc tgacacatgt cctccgtacg atgaagaagc ccgttctctg
    44101 gcggtggtca taaaaaaccg taacggcctg catgtacgtc cggcctcccg gctggtttat
    44161 accttatcga catttaatgc cgatatgttg ctggaaaaaa acggcaaatg cgtcacacca
    44221 gagagtatta accagattgc gttactacaa gttcgctata acgatacgct gcgcctgatt
    44281 gcgaaagggc cagaagctga agaggcactg atcgctttcc gtcacctggc tgaagataac
    44341 tttggtgaaa cggaggaagt cgctccacct actctgcgtc ccgttctgcc tgtttcgggt
    44401 aaagcctttt attatcaacc agttttatgt acggtacagg caaaatcaac cctgaccgcg
    44461 gaagaagaac aagatcgatt acgccaggct attgacttca cgttattaga tctgatgacg
    44521 ttaacagcga aagcagaagc cagcgggctt gacgatattg ccgcaatctt ttctggtcac
    44581 catacactgt taggtgatcc ggaactgctg gcggcggcaa gcgaactcct tcagcatgaa
    44641 cattgcacgg cagaatatgc ctggcagcaa gttcttaaag aacttagcca gcaataccag
    44701 caactggatg atgaatatct acaagctcgc tatattgatg tggacgatct tctgcatcgc
    44761 accctggtcc acctgaccca aacgaaagaa gaactcccgc agtttaactc gccaactatt
    44821 ctactggcgg agaacattta tccttccaca gtactgcaac tggatccggc ggttgtaaaa
    44881 ggtatctgcc ttagcgccgg aagtccggta tcccacagcg ccctaatcgc ccgtgaactg
    44941 gggattggct ggatttgcca gcagggtgag aaactgtatg cgatacaacc agaagaaacg
    45001 ctaacgctgg acgttaaaac gcaacgtttc aaccgtcagg gttaagccac cgttcggatg
    45061 gcatcgttct gatgtcatcc ggcaatttac ccagcactga tgttttttaa ctacctgtta
    45121 tattttttac caatttatga tgatttttct tcgctaatcg cgaaccaaat gtcgctggct
    45181 ggctaataaa cagacgttat ttgatcactc tatttcgccg ttatttatcc ctttaattcc
    45241 cttttctaaa atgcctgacc gttcgcagaa tgagatttcg atcatgcagc tagtgcgatc
    45301 ctgaactaag gttttctgat acttgaatac cgttttattc ggtttcgcca aaggagaatg
    45361 attgatgaaa tcccccgcac cttctcgccc gcaaaaaatg gcgttaattc cagcctgtat
    45421 ctttttgtgt ttcgctgcgc tatcggtgca ggcagaagaa acaccggtaa caccacagcc
    45481 gcctgatatt ttattagggc cgctgtttaa tgatgtgcaa aacgccaaac tttttccgga
    45541 ccaaaaaacc tttgccgatg ccgtgccgaa cagcgatccg ctgatgatcc ttgctgatta
    45601 tcggatgcag caaaaccaga gcggatttga tctgcgccat ttcgttaacg tcaatttcac
    45661 cctgccgaaa gaaggcgaga aatatgttcc gccagagggg cagtcactgc gcgaacatat
    45721 tgacggactt tggccggtat taacgcgttc taccgaaaac accgaaaaat gggattctct
    45781 gttaccgctg ccggaacctt atgtcgtgcc gggcggacgt tttcgcgagg tatattactg
    45841 ggacagttac ttcaccatgt taggacttgc cgaaagtggt cactgggata aagtcgcgga
    45901 tatggtggcc aattttgctc atgaaataaa cacttacggt catattccca acggcaaccg
    45961 cagttactat ttaagccgct cgcaaccgcc cttctttgcc ctgatggtag agttactggc
    46021 gcagcatgaa ggcgatgccg cgttgaagca atacctgccg caaatgcaaa aagaatatgc
    46081 ttactggatg gacggtgttg aaaacctgca agccggacaa caggaaaaac gcgttgtaaa
    46141 acttcaggat ggtacccttc tcaaccgcta ctgggacgat cgcgatacgc cacgaccaga
    46201 gtcatgggtg gaagatattg ccaccgccaa aagcaatccg aatcgacctg ccactgaaat
    46261 ttaccgcgac ctgcgctctg ccgctgcgtc tggctgggat ttcagctcgc gctggatgga
    46321 caacccgcag cagttaaata ccttacgcac caccagcatc gtaccggtcg atctgaacag
    46381 cctgatgttt aaaatggaaa aaatcctcgc ccgcgccagc aaagctgcag gagataacgc
    46441 gatggcaaac cagtacgaaa cgctggcgaa tgcccgtcaa aaagggatcg aaaaatacct
    46501 gtggaacgat caacaaggct ggtatgccga ttacgacctg aaaagtcata aagtgcgcaa
    46561 tcagttaacc gcggccgccc tgttcccgct gtacgtcaat gcggcagcga aagatcgcgc
    46621 caacaaaatg gcgacggcga cgaaaacaca tctgctgcaa cccggcggcc tgaacaccac
    46681 gtcggtgaaa agtgggcaac aatgggatgc gccaaatggc tgggcaccgt tacagtgggt
    46741 cgcgacagaa ggattacaaa actacgggca aaaagaggtg gcgatggaca ttagctggca
    46801 cttcctgacc aatgttcagc acacctatga ccgggagaaa aagctggtgg aaaaatatga
    46861 tgtcagcacc accggaacgg ggggcggcgg tggcgaatat ccattacagg atggctttgg
    46921 ctggaccaat ggcgtgacgc tgaaaatgct ggatttgatc tgcccgaaag agcaaccgtg
    46981 tgacaatgtt ccggcgacgc atccgaccgt taagtcagca acgacgcaac cctcaaccaa
    47041 agaggcacaa cccacacctt aaccagcgct tactccgaca acgtcattcg gcttctccgg
    47101 atggcgttgt cggcagataa caccatatct atggtttttt ctacacagac ggtttttcaa
    47161 gtcctggcgg gaagtattgc gcaaatgttt gccatacgtg gttgccgaag atagcgtcat
    47221 ttctacgggt aagtgtttca ctttcatcac ccggcgattt acgccgacgt tggctatata
    47281 aagcatcaat tgctaccgaa tattgttacg gtgttcactc atcaacgttt ttttgctttc
    47341 tggagttaaa ttaataatca cataatccag acactccaga gaatttagca accaaaaaaa
    47401 gaattcagac cattcctctt cgcgtaactg aagcattgcc gaacgggtat tttggcttaa
    47461 tctgccagaa ggattgatca attctaaatg ttcaataagc gcagccaaaa ccgcctcact
    47521 atccgcccca tttttaacgc ctgactggct actgtctaaa atatttattt tctgaatcac
    47581 accaaaaaca acgaaacaat aattaaaaaa ggattctgtt gttgttaccg ggaaccgact
    47641 tatttcgctc agaaagtcgt tcaaccagct ccgcagtgat aagattcgct gatgatacag
    47701 tgtatcctca ctgcgtgctg gaggtgccag tataatatgt tgatcaatac agtatttatt
    47761 gtgttcagtc gtttcctgat gcgtttggtt ctctgcggat tgtcgctggc gaaataaatg
    47821 gcttgcggta aatgccgtta cagagcagcg caacgcgtca attccttcgg agtgcccggc
    47881 ttttggcgag agttccggtt ttatgcgtcc aggtagcgga aatttcaccc gaatgagaaa
    47941 tgcattaata aaatg
//
LOCUS       DAECNO010000019        47943 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.019, whole genome shotgun sequence.
ACCESSION   DAECNO010000019 DAECNO010000000
VERSION     DAECNO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.019"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1226
                     /locus_tag="KKO08_001090"
     CDS             <1..1226
                     /locus_tag="KKO08_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD6317423.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1223..2188
                     /gene="yggF"
                     /locus_tag="KKO08_001091"
     CDS             1223..2188
                     /gene="yggF"
                     /locus_tag="KKO08_001091"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBD6317424.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2210..2719
                     /gene="fumE"
                     /locus_tag="KKO08_001092"
     CDS             2210..2719
                     /gene="fumE"
                     /locus_tag="KKO08_001092"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBD6317425.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2716..3429
                     /locus_tag="KKO08_001093"
     CDS             2716..3429
                     /locus_tag="KKO08_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBD6317426.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3401..4083)
                     /locus_tag="KKO08_001094"
                     /pseudo
     CDS             complement(3401..4083)
                     /locus_tag="KKO08_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4077..4754)
                     /locus_tag="KKO08_001095"
     CDS             complement(4077..4754)
                     /locus_tag="KKO08_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBD6317427.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4742..5125)
                     /locus_tag="KKO08_001096"
     CDS             complement(4742..5125)
                     /locus_tag="KKO08_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBD6317428.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5141..5449)
                     /locus_tag="KKO08_001097"
     CDS             complement(5141..5449)
                     /locus_tag="KKO08_001097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317429.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5450..6028)
                     /locus_tag="KKO08_001098"
     CDS             complement(5450..6028)
                     /locus_tag="KKO08_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBD6317430.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6051..6482)
                     /locus_tag="KKO08_001099"
                     /pseudo
     CDS             complement(6051..6482)
                     /locus_tag="KKO08_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6854..7873
                     /gene="epd"
                     /locus_tag="KKO08_001100"
     CDS             6854..7873
                     /gene="epd"
                     /locus_tag="KKO08_001100"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBD6317431.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7923..9086
                     /gene="pgk"
                     /locus_tag="KKO08_001101"
     CDS             7923..9086
                     /gene="pgk"
                     /locus_tag="KKO08_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBD6317432.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9301..10380
                     /gene="fbaA"
                     /locus_tag="KKO08_001102"
     CDS             9301..10380
                     /gene="fbaA"
                     /locus_tag="KKO08_001102"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBD6317433.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10571..11431
                     /gene="mscS"
                     /locus_tag="KKO08_001103"
     CDS             10571..11431
                     /gene="mscS"
                     /locus_tag="KKO08_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBD6317434.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11570..12205
                     /gene="argO"
                     /locus_tag="KKO08_001104"
     CDS             11570..12205
                     /gene="argO"
                     /locus_tag="KKO08_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBD6317435.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12298..13038
                     /locus_tag="KKO08_001105"
     CDS             12298..13038
                     /locus_tag="KKO08_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBD6317436.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13205..14155
                     /locus_tag="KKO08_001106"
     CDS             13205..14155
                     /locus_tag="KKO08_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6317437.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14097..15575)
                     /gene="scpC"
                     /locus_tag="KKO08_001107"
     CDS             complement(14097..15575)
                     /gene="scpC"
                     /locus_tag="KKO08_001107"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBD6317438.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15599..16375)
                     /gene="scpB"
                     /locus_tag="KKO08_001108"
     CDS             complement(15599..16375)
                     /gene="scpB"
                     /locus_tag="KKO08_001108"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBD6317439.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16386..17381)
                     /gene="meaB"
                     /locus_tag="KKO08_001109"
     CDS             complement(16386..17381)
                     /gene="meaB"
                     /locus_tag="KKO08_001109"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBD6317440.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17374..19518)
                     /gene="scpA"
                     /locus_tag="KKO08_001110"
     CDS             complement(17374..19518)
                     /gene="scpA"
                     /locus_tag="KKO08_001110"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBD6317441.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19722..20615)
                     /gene="argP"
                     /locus_tag="KKO08_001111"
     CDS             complement(19722..20615)
                     /gene="argP"
                     /locus_tag="KKO08_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBD6317442.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20998..21657
                     /gene="rpiA"
                     /locus_tag="KKO08_001112"
     CDS             20998..21657
                     /gene="rpiA"
                     /locus_tag="KKO08_001112"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBD6317443.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21913..23145
                     /gene="serA"
                     /locus_tag="KKO08_001113"
     CDS             21913..23145
                     /gene="serA"
                     /locus_tag="KKO08_001113"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBD6317444.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23374..23433
                     /gene="ibsC"
                     /locus_tag="KKO08_001114"
     CDS             23374..23433
                     /gene="ibsC"
                     /locus_tag="KKO08_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBD6317445.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23766..23825
                     /locus_tag="KKO08_001115"
     CDS             23766..23825
                     /locus_tag="KKO08_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD6317446.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23926..24528)
                     /locus_tag="KKO08_001116"
     CDS             complement(23926..24528)
                     /locus_tag="KKO08_001116"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBD6317447.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24549..24732)
                     /gene="ssrS"
                     /locus_tag="KKO08_001117"
     ncRNA           complement(24549..24732)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KKO08_001117"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24774..25103)
                     /gene="zapA"
                     /locus_tag="KKO08_001118"
     CDS             complement(24774..25103)
                     /gene="zapA"
                     /locus_tag="KKO08_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBD6317448.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25271..25849
                     /gene="ygfB"
                     /locus_tag="KKO08_001119"
     CDS             25271..25849
                     /gene="ygfB"
                     /locus_tag="KKO08_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBD6317449.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25875..27200
                     /gene="pepP"
                     /locus_tag="KKO08_001120"
     CDS             25875..27200
                     /gene="pepP"
                     /locus_tag="KKO08_001120"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBD6317450.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27197..28375
                     /gene="ubiH"
                     /locus_tag="KKO08_001121"
                     /gene_synonym="visB"
     CDS             27197..28375
                     /gene="ubiH"
                     /locus_tag="KKO08_001121"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBD6317451.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28398..29600
                     /gene="ubiI"
                     /locus_tag="KKO08_001122"
                     /gene_synonym="visC"
     CDS             28398..29600
                     /gene="ubiI"
                     /locus_tag="KKO08_001122"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBD6317452.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29674..29757)
                     /locus_tag="KKO08_001123"
     CDS             complement(29674..29757)
                     /locus_tag="KKO08_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317453.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30048..31142
                     /gene="gcvT"
                     /locus_tag="KKO08_001124"
     CDS             30048..31142
                     /gene="gcvT"
                     /locus_tag="KKO08_001124"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBD6317454.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31166..31555
                     /gene="gcvH"
                     /locus_tag="KKO08_001125"
     CDS             31166..31555
                     /gene="gcvH"
                     /locus_tag="KKO08_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBD6317455.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31674..34547
                     /gene="gcvP"
                     /locus_tag="KKO08_001126"
     CDS             31674..34547
                     /gene="gcvP"
                     /locus_tag="KKO08_001126"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBD6317456.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34814..35556
                     /locus_tag="KKO08_001127"
                     /pseudo
     CDS             34814..35556
                     /locus_tag="KKO08_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35613..37046)
                     /gene="bglA"
                     /locus_tag="KKO08_001128"
     CDS             complement(35613..37046)
                     /gene="bglA"
                     /locus_tag="KKO08_001128"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBD6317457.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37091..37401
                     /locus_tag="KKO08_001129"
                     /pseudo
     CDS             37091..37401
                     /locus_tag="KKO08_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37565..38224
                     /locus_tag="KKO08_001130"
     CDS             37565..38224
                     /locus_tag="KKO08_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBD6317458.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="KKO08_001131"
     CDS             complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="KKO08_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBD6317459.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39643..39909
                     /gene="sdhE"
                     /locus_tag="KKO08_001132"
     CDS             39643..39909
                     /gene="sdhE"
                     /locus_tag="KKO08_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBD6317460.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39890..40297
                     /locus_tag="KKO08_001133"
     CDS             39890..40297
                     /locus_tag="KKO08_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBD6317461.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="KKO08_001134"
     CDS             complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="KKO08_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBD6317462.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40970..41866
                     /gene="xerD"
                     /locus_tag="KKO08_001135"
     CDS             40970..41866
                     /gene="xerD"
                     /locus_tag="KKO08_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBD6317463.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41891..42601
                     /gene="dsbC"
                     /locus_tag="KKO08_001136"
     CDS             41891..42601
                     /gene="dsbC"
                     /locus_tag="KKO08_001136"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBD6317464.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42607..44340
                     /gene="recJ"
                     /locus_tag="KKO08_001137"
     CDS             42607..44340
                     /gene="recJ"
                     /locus_tag="KKO08_001137"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBD6317465.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44431..45529
                     /gene="prfB"
                     /locus_tag="KKO08_001138"
     CDS             join(44431..44505,44507..45529)
                     /gene="prfB"
                     /locus_tag="KKO08_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBD6317466.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45539..47056
                     /gene="lysS"
                     /locus_tag="KKO08_001139"
     CDS             45539..47056
                     /gene="lysS"
                     /locus_tag="KKO08_001139"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBD6317467.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47099..47647)
                     /gene="idi"
                     /locus_tag="KKO08_001140"
     CDS             complement(47099..47647)
                     /gene="idi"
                     /locus_tag="KKO08_001140"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBD6317468.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47702..47773
                     /locus_tag="KKO08_001141"
     CDS             47702..47773
                     /locus_tag="KKO08_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317469.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47770..47895)
                     /locus_tag="KKO08_001142"
     CDS             complement(47770..47895)
                     /locus_tag="KKO08_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317470.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
BASE COUNT        11472 a        12015 c        12962 g        11494 t
ORIGIN      
        1 aatttgaact gccagaaatt accgataatg aactgttagt gagtgtaatt tctgacagcg
       61 tctgtttatc gacctggaaa gcggcgttac tcggtagtga acataaacgc gtacccgacg
      121 atttagaaaa tcatccggtc attaccgggc atgaatgtgc cggggttatt gtcgaagtgg
      181 gtaaaaatct cactggcaaa tataaaaaag gccagcgttt tgtattgcaa ccggcgatgg
      241 ggttaccaag cggatattca gcgggctaca gctacgaata ttttggcggc aatgccactt
      301 atatgattat tcccgaaatc gccattaatt tgggctgcgt attaccgtat cacggctctt
      361 attttgctgc ggcgtcgctg gcagagccta tgtgctgcat tattggtgct tatcatgcca
      421 attatcacac cacgcaatat gtttatgagc atcgcatggg cgtcaaacct ggcggcaata
      481 ttgcactgct ggcgtgtgcg ggtccgatgg gcattggcgc tatcgattac gccattaacg
      541 gtggcataca accgtcacgg gtggtggtgg tcgatatcga cgacaaacgc ctggcgcagg
      601 tgcagaagct gctgccggtg gatctggcgg ccagtaaagg cattgagcta gtgtatgtga
      661 ataccaaagg gatgagcgat cctatccaga tgctgcgggc gctgacagga gatgtcgggt
      721 tcgatgacat ttttgtttat gcggcggtgc ctgctgtcgt tgagatggct gacgaattac
      781 tggcggaaga tggctgtctg aacttctttg ccgggctgac ggataaaaac ttcaaagtgc
      841 cgtttaattt ctacaacgtc cattacaaca gcacgcacgt agtcggtaca tccggcggtt
      901 caacggacga catgaaagag gcgattgccc ttagcgccac aggacagtta cagccgtcgt
      961 ttatggtgac ccatattggt ggcctggatg cggtgccaga aaccgtgctc aatctgccgg
     1021 atatccctgg cggtaaaaaa ctcatttata acggcgtgac catgccgctc actgccattg
     1081 ccgattttgc cgaaaaaggc aaaaccgatc cgctgtttaa agagttggcg cggctggttg
     1141 aggaaacgca cggcatctgg aatgaacagg ccgagaaata tctgctggca caatttggcg
     1201 ttgatatcgg ggaggccgcg caatgatgtc cctggcgtgg ccattatttc gcgttacgga
     1261 acaggcagcg ttggctgcct ggccgcacac cggatgtggc gacaaaaata aaattgatgg
     1321 cctggcggtc accgcgatgc gccaggcatt aaacgacgtc gcttttcgtg gacgagtggt
     1381 tatcggcgaa ggagaaattg accatgcgcc gatgctgtgg attggcgaag aggtgggcaa
     1441 aggcgatggg ccagaagtcg atatcgcggt tgatcccatt gaaggcacgc ggatggtggc
     1501 gatggggcag agcaatgcgc tggcggtcat ggctttcgct cctcgagata gcctgttaca
     1561 tgcgcccgat atgtatatga aaaagctggt tgttaatcga ctggctgccg gggcgattga
     1621 tctgtctttg cctctggcgg acaacttgcg taacgtggcg cgggcgttag gtaaaccgct
     1681 ggacaagctg cgcatggtta cgctggataa accgcgtctg agtgccgcga ttgaagaagc
     1741 aacacagctg ggcgtgaagg tttttgccct gccggatggc gatgtcgccg ccagcgtgct
     1801 gacctgctgg caggataatc cttacgatgt gatgtacacc atcggcggtg cgccagaagg
     1861 cgtgatttct gcctgtgccg ttaaagcgtt aggcggggat atgcaggccg agctgattga
     1921 cttttgtcag gctaaagggg attacacgga aaatcggcag attgcagagc aggagcgcaa
     1981 gcgttgtaag gcaatgggcg ttgacgttaa ccgtgtgtac tcgctcgatg aactggtgag
     2041 ggggaacgat atcctcttta gcgctacggg cgtgacgggc ggcgagttgg tgaacggtat
     2101 ccaacagacg gcgaatgggg tgcggacgca gacattactg atcggcggcg cggaccaaac
     2161 gtgtaatata atagactccc tgcattgatg gtgatctatc ggaagaccaa tggcgacgct
     2221 gacagaagat gatgtgcttg agcaactgga tgcacaggac aatttatttt catttatgaa
     2281 aactgcgcat tctattttgc tccaggggat acgccagttt ctgccgtcgc tgtttgtcga
     2341 taacgatgaa gagatcgtcg aatatgcagt gaagccgtta ctcgcccaaa gcggcccgct
     2401 tgacgatatt gatgttgcgc tgcgtttgat ttatgcgctg gggaaaatgg ataaatggct
     2461 gtatgccgat atcacgcact tttcccagta ctggcattac ctgaacgaac aggatgaaac
     2521 gcccggattt gccgatgata taacctggga ttttatcagc aatgtcaaca gtataactcg
     2581 caatgcgacg ctctacgatg cgttaaaagc gatgaagttc gccgatttcg ccgtctggtc
     2641 agaggcgcgt tttagcggaa tggtcaaaac ggcgctgacg ctggcagtaa cgaccacctt
     2701 aaaggaatta acgccgtgaa aattgaatta acggtgaatg ggctgaacgt gcaagcgcag
     2761 taccatgatg aagagataga acgcgtgcat aaactgttgc tgcgtatgct ggcggcctta
     2821 cagacggtaa atccgcagcg gcgtacggtg gtttttcttt gcgctccgcc ggggacgggt
     2881 aaatctaccc taaccacctt ctgggaatat ctcgcgcaac aagacccgga gctgcccgct
     2941 attcaggcgc tcccgatgga tggttttcac cattacaata gctggctgga cgcgcatcaa
     3001 ttgcgcccct tcaaaggcgc accagagaca tttgacgttg cgaaactggc ggaaaatctg
     3061 cgccaggtgg tggaagggga ttgtacgtgg ccgcagtacg atcgacaaaa gcatgatcct
     3121 gttgaagatg cgttgcacgt taccgcacca ctcgtcatcg tcgaaggaaa ctggttgttg
     3181 ctggacgacg agaagtggtg ccagcttgcg caattttgtg atttctcgat ctttatcaac
     3241 gcgccagctg ctgcgttgcg ggagcgtctg gtggggcgca agctggcggg aggggtatcg
     3301 ctggcagatg cagaagcctt ttatgaccgt accgacgggc cgaatgttcg ccgggtgctg
     3361 gaagagagtc tgcctgcaaa tttgacctta atgatgaccg ctacgggtga atatcgtctg
     3421 gtggattaac gttcctgatt acaccatctt ccagccgcac cacacgagag aaatgacgcc
     3481 gggtaaatgc ggcgtcatga ctgattgcta ccacgctcgt gcctcgctgg cggcacttct
     3541 ccagccagct ttcaaacacg ctcaaccaat tttcgtcaaa atcccgactc ggctcgtcca
     3601 gcagtaatag tggcggagag agtgcttcca ggcaggcgac ggcgaccatc cgtcgctgcg
     3661 cactatgcag atccaacggg tgcgagttgg cagtgtctgc taactgacaa cattgcaggg
     3721 cagcatgggt acgttgagtg atttcatccg ccgggcattt ttgcagtttc aaaccaaatg
     3781 cgatctcgtc agcgacggtg ctgtgaaaaa gttggttttc cgcttcctga aacagaacag
     3841 tacgccaacc tttgccgcgc gctggcggtt tttcaggttt ttcatcgcct gttgttgcag
     3901 cataaccgta ccggctgtgg gcgtaagaag acctgccatt actcgtaaca gcgtcgattt
     3961 ccctgcgcca ttgtcaccgg tcagcgccaa ccattcacct tgtttgagtt gcagagaaat
     4021 gtcgcaaaga caatccgttg ccgcgccagg ccagcgataa gatatcttat ttaacgttaa
     4081 catagtggct ggagcgctcc gttttgcaat tgccacgctt gctggcagta gttcaggaaa
     4141 ggtgtgcgat gacgttcaaa aagaataatc agcgaaccac gttcaaacgc ccagtgttgt
     4201 aagcgctgca acagcatttc gctggcctgc ggcgtcaggc ggctaaaggc ttcatcaaga
     4261 attaagagct ttggctgcat cgcgatggcg caggcgatga tcacgcgctg tgtctctcca
     4321 ccggagagtg ttgccgggtg acgatggcgc aacggctggc actcggtcag agtcagggcg
     4381 gcatcaatcc gcgccataat ctctgcttca gcaagacaca gattttctgg cccaaaggca
     4441 acttcttctt ccacgctaaa ggtacagccg gagagctgca aatagggcga ttgctggacg
     4501 agctggatgg tggcggattg ttcattaagc gggagatgcc caatgggcgt accgaggagc
     4561 gtccccgtgc ctgttatctc tcttggcaga aagtccggat accagcccgc catcagctgt
     4621 gccagcgtac ttttgccact gccgttgtca ccaaagatgg cgaccatccc cggtttgaca
     4681 tagtgaaagt cataacagaa cggaggatgc gttgagtgtg tcgggcaata acggaactgt
     4741 tctaacgtaa ccatatccac gctccgaatt ctgtcagcat cagcaagatc atggtgtaac
     4801 gcgctacgcg ttgtagggtg ctatcggctg gtgcccacaa cgttgttcgt cggttgtgga
     4861 tccgaaatgc ccgcatatcc agcgccgcac cacggacagc cagatcgtta agcgcattgt
     4921 gtgttagcgg aataattaac gcgggcatag cacgtaaacg ctgataccag ccctcatcca
     4981 gcggcacgcc gcgggcacgc tgcgcttcat gaataatcgc taattgccgt tttaactgct
     5041 caacgaccaa taacggcccg gcaaacagat aagcgacgcc tggcggcagg cgagaagcaa
     5101 acagagcgcg aataaaacat tgcacgggga cgtactgcat tcacaactgt gaggttgaaa
     5161 caatcgccag aatcagcaac cagagcgtaa tagcatgcga ccaccgttct ggcgagcgtg
     5221 gcgtgccgct tagccattct ggtagccagc caccgtgtac cagccacagc ccggctccca
     5281 gcgagaacat cagccagacg acatattttg cccgccgccg cgtggccttt agcgcaataa
     5341 ggcagaaaaa ggtcgccgcg ctatagatcg gcaaaatggt ctgcgcgggc aggataagcg
     5401 tggtgcaggc cgccaatgcc cacagtgtta atgaggtaaa tggatgcatt tagcgcacgg
     5461 cagccattgc cggaaaatgg cgcgtggtgc gaatcggcag ctggcgcagt aaaagccaga
     5521 caatcaccgc tgtaagaatt ttatcgacca ggttcgcgcc tatcaccgta atcgccaccg
     5581 actccaccag gttttgcccc atcgagtgca tccaggcaac gaacaaatcg gctccgctac
     5641 cggtgacacc gccgaacagc gcagtacgca gcggtacagc gacgaccgtt accgcaagcg
     5701 taataatcac gccgctgaca acgacttttg gcagggtgcg aaaccaaccc gctcgcgcca
     5761 gccaaccgga gaccaggccg atgaccatcg cgacgggtgc aaatgcggcg gcgatcgggt
     5821 cggtcagcaa tccccagagt aaattggtca gtaagccggt cagcattccg atcaccggac
     5881 caagcagtac cgcgctaata agtgtgccga tggagtcgag aaatataggt aatttgacca
     5941 tgctggccag ttgcccgccg atcatattga tggcgataga tataacgatc aacaccagtg
     6001 cctgactgga aaagtggcta cgcgccataa gaaaacctct ttgaaagtag ttatttaacg
     6061 ggggaaatat gcaactcgtc gtaaccagaa agcgcgcaag gttggcggct aaacgccagt
     6121 tcctcaaggc taccgatcac cagttcaagc gtggtccgca cggtacagcc gggcatcata
     6181 tgaccgccaa gcatcgtgcc gtgtggatcg gaaacgcaga gatgaagatg ctcaccactc
     6241 tgttccagcg tgccgtttag tgagatcact tcgaatgtac cgttcaggag cgtggtgcct
     6301 tcttgcccgg catagcgcaa ggcgacgtca gtcaggctac cggtacagcc tgcaatctag
     6361 gcggcatgaa gttgatgttg ctgtgcaaaa gcgtgtagct gggaaaaaac ttcctgcccg
     6421 gggaggaggc gcagtgcgta aaagcgtgcg gaggaagaat atgagtaggg caatgtcatc
     6481 attggagtac tccgaaactc ggacgtattg cggataatat cgacgctgca tcagtcatat
     6541 tctgatataa gtcagggttt gtgacggcac aacgaaaaga ttttgctacg cttattcgtg
     6601 attctgcaaa gtggaataaa ttccacaggg gcggggctga aaacgtgacg taagtcagat
     6661 aattgctttc tttgactgga caatcattcc ttttattcca cgtttcgctt atcctagctg
     6721 aagcgtttca gtcgattaaa tgttcgacaa ttaaccaatc agtcgcagtt tgcgacaggt
     6781 aaggtttccc cggacgattt gctggattac tctgtctggc aaatttgctc agggaaaacc
     6841 ttgcaggaga tctatgaccg tacgcgtagc gataaatggc ttcggtcgca tcgggcgtaa
     6901 tgtggttcgt gctttgtatg aatccggacg ccgggcggaa attaccgtgg tggcaatcaa
     6961 cgaactggcg gatgctgcgg gcatggcgca tttgttgaaa tatgacacca gccatggccg
     7021 ttttgcatgg gaagtacgac aggaacgcga tcaacttttt gttggtgatg acgccatccg
     7081 cgtattgcat gaacgttcac tgcaatcgct cccctggcgt gaacttggcg ttgatgtagt
     7141 cctcgactgc accggcgtat atggctcccg cgagcatggc gaagcgcata ttgccgccgg
     7201 ggccaaaaaa gtgctctttt cacatcctgg cagtaacgat ctcgacgcga ccgttgttta
     7261 cggcgtcaat caggatcaac ttcgtgcgga acaccgcatc gtttctaacg cttcctgtac
     7321 cacgaattgc ataattcccg tcatcaaatt gttagatgat gcgtacggta ttgagtccgg
     7381 cactgtgacc acaattcact ccgccatgca cgatcaacag gttattgatg cataccatcc
     7441 tgacctgcgt cgcacccggg cagccagcca gtcgatcatt ccggtcgata ctaaactggc
     7501 cgctggtatc acacgatttt ttccgcaatt taacgatcgc tttgaagcga ttgcggtacg
     7561 tgtgccaacc ataaatgtga cggcaatcga tttaagcgtg acggtgaaga aacctgtaaa
     7621 agccaatgaa gtcaacctgt tgctgcaaaa agcagcacaa ggtgcatttc atggtatagt
     7681 tgactatacg gaattgccgt tggtctctgt agattttaac cacgatccgc acagtgccat
     7741 tgtcgatggc acccaaaccc gggtcagtgg cgcacacctg atcaaaacgt tggtctggtg
     7801 cgataacgaa tggggctttg ctaaccgaat gctcgacacg acgttagcta tggctactgt
     7861 tgctttcagg taagacgcaa gcagcgtctg caaaactttt agaatcaacg agaggattca
     7921 ccatgtctgt aattaagatg accgatctgg atcttgctgg gaaacgtgta tttatccgtg
     7981 cggatctgaa cgtaccagta aaagacggga aagtaaccag cgacgcgcgt atccgtgctt
     8041 ctctgccgac catcgaactg gccctgaaac aaggcgcaaa agtgatggta acttcccacc
     8101 tgggtcgtcc taccgaaggc gagtacaacg aagaattctc tctgctgccg gttgttaact
     8161 acctgaaaga caaactgtct aacccggttc gtctggttaa agattacctc gacggcgttg
     8221 acgttgctga aggtgaactg gttgttctgg aaaacgttcg cttcaacaaa ggcgagaaga
     8281 aagacgacga aaccctgtcc aaaaaatacg ctgcactgtg tgacgtgttc gtaatggacg
     8341 cattcggtac tgctcaccgc gcgcaggctt ctactcacgg tatcggtaaa ttcgctgacg
     8401 ttgcgtgcgc aggcccgctg ctggcagctg aactggacgc gctgggtaaa gcactgaaag
     8461 aacctgctcg cccgatggtg gctatcgttg gtggttctaa agtatctacc aaactgaccg
     8521 ttctggactc cctgtctaaa atcgctgacc agctgattgt tggtggtggt atcgctaaca
     8581 cctttatcgc ggcacaaggc cacgatgtgg gtaaatccct gtacgaagct gacctggttg
     8641 acgaagctaa acgtctgctg accacctgca acatcccggt tccgtctgat gttcgcgtag
     8701 caaccgagtt ctctgaaact gcaccggcta ccctgaaatc tgttaacgat gtgaaagctg
     8761 acgagcagat cctggatatc ggtgatgctt ccgctcagga actggctgaa atcctgaaga
     8821 atgcgaaaac cattctgtgg aacggtccgg ttggcgtgtt cgaattcccg aacttccgca
     8881 aaggtactga aatcgtggct aacgctatcg cagacagcga agcgttctcc atcgctggcg
     8941 gcggcgacac tctggcagca atcgacctgt tcggcattgc tgacaaaatc tcctacatct
     9001 ccactggcgg cggcgcattc ctcgaattcg tggaaggtaa agtactgcct gcagtagcga
     9061 tgctcgaaga gcgcgctaag aagtaaaaaa tcacagggca gggaaacctg cacttgtttc
     9121 agcgcgcttt tagagcacgc attttttcat actctaaata attcgagttg caggaaggcg
     9181 acaagagagt gaatccccag gagcttacat aagtaagtga ctggggggag cgaacgcaga
     9241 cgcagcacat gcaacttgaa gtattatgag tataaggccc gacgatacag gacaagagac
     9301 atgtctaaga tttttgattt cgtaaaacct ggcgtaatca ctggtgatga cgtacagaaa
     9361 gttttccagg tagcaaaaga aaacaacttc gcactgccag cagtaaactg cgtcggtact
     9421 gactccatca acgccgtact ggaaactgct gctaaagtta aagcgccggt tatcgttcag
     9481 ttctccaacg gtggtgcttc ctttatcgct ggtaaaggcg tgaaatctga cgttccgcag
     9541 ggtgctgcta tcctgggcgc gatctctggt gcgcatcacg ttcaccagat ggctgaacat
     9601 tatggtgttc cggttatcct gcacactgac cactgcgcga agaaactgct gccgtggatc
     9661 gacggtctgc tggacgcggg tgaaaaacac ttcgcagcta ccggtaagcc gctgttctct
     9721 tctcacatga tcgacctgtc tgaagaatct ctgcaagaga acatcgaaat ctgctctaaa
     9781 tacctggagc gtatgtccaa aatcggcatg actctggaaa tcgaactggg ttgcaccggt
     9841 ggtgaagaag acggcgtgga caacagccac atggacgctt ctgcactgta cacccagccg
     9901 gaagacgttg attacgcata caccgagctg agcaaaatca gcccgcgttt caccatcgct
     9961 gcgtccttcg gtaacgtaca cggtgtttac aagccgggta acgtggttct gactccgacc
    10021 atcctgcgtg attctcagga atatgtttct aagaaacaca acctgccgca caacagcctg
    10081 aacttcgtat tccacggtgg ttccggttct actgctcagg aaatcaaaga ctccgtaagc
    10141 tacggcgtag tgaaaatgaa catcgatacc gatacccaat gggcaacctg ggaaggcgtt
    10201 ctgaactact acaaagcgaa cgaagcttat ctgcagggtc agctgggtaa cccgaaaggc
    10261 gaagatcagc cgaacaagaa atactacgat ccgcgcgtat ggctgcgtgc cggtcagact
    10321 tcgatgatcg ctcgtctgga aaaagcattc caggaactga acgcgatcga cgttctgtaa
    10381 gtcattcctt tctgcttatc tcaaggcccg ctctgcgggt ctttttttcg ccaaaagcaa
    10441 ggtgattcag atgagaaatc tgtgatctat ttggcaaaat tatgctttat tgtttaccct
    10501 tgtcagactg cccgtcataa ggcggcggag tgtatttctc cattttgagt cagttgaaaa
    10561 ggaatattga atggaagatt tgaatgttgt cgatagcata aacggcgcgg gaagctggct
    10621 ggtagctaac caggcgctgc tgctaagtta tgcagtaaac atcgtggcgg cactcgcgat
    10681 catcatcgtt ggtttgatta tcgcgcggat gatttccaac gcggtgaatc gcctgatgat
    10741 ctcccgtaaa atcgatgcca ctgttgctga ttttctttct gcattagtcc gttacggtat
    10801 tatcgccttt acgctaatcg ctgccctggg acgcgtgggt gtacaaacag cgtcagtcat
    10861 tgctgtactc ggtgccgcag gcttagctgt tggtctggct ttgcaggggt cactttctaa
    10921 cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggag aatatgttga
    10981 cctcggcggc gtagccggta ctgtgctgag tgtgcagatt ttctccacca ccatgcgtac
    11041 tgcagacggt aaaattatcg ttattccgaa cggtaaaatt attgccggaa atattattaa
    11101 cttctcccgc gagccagttc gccgtaacga atttattatt ggcgtggcgt atgattccga
    11161 tatcgatcag gttaagcaga tcctgaccaa tattatccag tctgaagatc gcattttgaa
    11221 agatcgcgaa atgactgtgc gcctgaacga acttggtgca tcgtcgatta atttcgtggt
    11281 ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc tggagcgtat
    11341 taaacgtgaa tttgatgccg ccggtatcag cttcccgtac ccgcaaatgg atgtgaactt
    11401 taagcgggtg aaagaagaca aagctgcgta atcaacgctg aggccagata atactctctg
    11461 gcctctctct tattagttat tctgattgcc aattaatatt atcaatttcc gctaataaca
    11521 atcccgcgat atagtctctg catcagatac ttaattcgga atatccaacg tgttttctta
    11581 ttactttcaa ggtcttgctc ttggggcagc tatgattctg ccgctcggcc cacaaaatgc
    11641 ttttgtgatg aatcagggca ttcgtcgtca gtaccacatt atgattgcct tactttgcgc
    11701 tatcagcgat ttggtcctga tttgcgcggg gatttttggt ggtagcgcgt tattgatgca
    11761 gtcgccgtgg ttgctagcgc tggtcacctg gggcggcgta gtcttcttgc tctggtatgg
    11821 ttttggcgct tttaaaacag cgatgagtag taatattgag ttagctagtg ccgaagtcct
    11881 gaagcaaggc agatggaaaa ttatcgccac catgttggca gtgacctggc tgaatccgca
    11941 tgtttacctg gatacttttg ttgtactggg cagccttggc gggcagcttg acgtcgaacc
    12001 aaaacgttgg tttgcgctcg ggacaattag cgcctctttc ctgtggttct ttggtctggc
    12061 tattctcgca gcctggctgg caccgcgcct gcgcacggca aaatcacagc gcattatcaa
    12121 tctggtagtg ggatgtgtca tgtggtttat agccttgcag ctggcgagag acggtattgc
    12181 tcatgcacaa gccttgttca gttaggcgct atctgatgga aaaataaaac agaggcgcta
    12241 agcttgcctc cagaggtcct gaattttggg caagtcaatt aacacggaga gactaacgtg
    12301 aagttcaaag ttatcgccct ggcggcatta atgggtatta gcgggatggc agcgcaggct
    12361 aacgaattgc cggatggacc gcatattgtc acctccggta cggcaagtgt ggatgcggtg
    12421 ccagacattg ccactcttgc gattgaagtt aacgtggccg cgaaggatgc cgctacagcc
    12481 aagaaacagg cagatgagcg cgtcgcacaa tacatttcct tccttgaact caatcagatc
    12541 gcgaaaaaag atatcagctc agcgaattta cgcacccagc cagattatga ctatcaggat
    12601 ggtaaaagta tcctgaaagg ctaccgagct gtgagaacgg tggaagtcac gctccgtcag
    12661 ttagacaaac tgaattcctt gctggatggc gcgctgaagg cgggtcttaa cgaaattcgt
    12721 tctgtgtcgc tgggcgtggc gcagccggat gcctataaag acaaagcgcg taaggcagcg
    12781 attgataacg cgattcatca ggcgcaggaa ctggcgaacg gctttcatcg taaactgggg
    12841 ccggtatata gcgtgcgcta ccatgtttcc aactatcagc ccagcccaat ggtgcggatg
    12901 atgaaagccg atgccgcgcc ggtgtctgcc caggaaactt acgagcaggc cgctattcag
    12961 tttgatgatc aggtcgatgt ggtcttccag ttagaacctg tggatcaaca acccgctaaa
    13021 acacctgcag cacaataata cttgtagccc gacgtattcg tcgggctaat gcctttaccc
    13081 gatattgcct cctttttctg taattttctg taaaaccatg acgtaatcac ctatctttgc
    13141 tcgcggtttc atcaaggata ggctgactga aacggttaag cggacaagga tgcggggagg
    13201 ggggatggac atttttattt cgaaaaagat gcgcaatttc attttattag cgcaaaccaa
    13261 taatattgcc cgggcggcag aaaaaatcca tatgacggct tcgccatttg gtaaaagcat
    13321 tgccgcgctg gaagaacaaa ttggctatac gctatttacc cgcaaagata ataatatcag
    13381 cctcaataaa gccggtcagg aactgtatca aaaactgttc ccggtttatc aacgactttc
    13441 tgcgattgat aatgaaatcc ataactcagg gcgtcgttca cgagaaattg tgattggcat
    13501 agataatact tatccgacaa ttattttcga tcagttgatt agtctcggag ataagtatga
    13561 aggcgtgact gcccagccag ttgagttcag cgaaaacggc gttatcgata atctttttga
    13621 ccgtcagtta gattttatta tctccccaca gcatgtatct gcacgagttc aggaactgga
    13681 aaacctgact atcagtgaat taccaccgct gcgattgggc tttctcgttt ctcgccgtta
    13741 tgaagagcgt caagagcaag agctgcttca ggagctgccc tggctacaaa tgcgctttca
    13801 aaaccgggct aacttcgaag caatgattga tgcgaatatg cgaccttgtg ggatcaatcc
    13861 caccattatt tatcgtccct acagttttat ggccaaaatc agcgccgtgg agcgcggaca
    13921 ttttctgacg gtgatcccgc attttgcctg gcgactggtg aatccggcaa cgctgaaata
    13981 ttttgatgcg ccgcacaggc cgatgtatat gcaggaatat ctttattcaa tcagaaatca
    14041 tcgttatacc gccacgatct tcagcatatt gctgaagatc gtgacgggac aaaccattaa
    14101 cccagcatcg agccggttgc aattaaatta cggtgtaagt cgaagacgtg gctaagatcg
    14161 tggtgaatat gtccgccggg cgcattttcc agatagcgat gcagataatc ccgatacata
    14221 ggatgtgcac aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag accgcgcaga
    14281 tcggcgatcc cttgttcggt gatgatcact ttgacgctgt gttcgctgtg atcaacatgg
    14341 ctgcacattg gcacgacggt tgagatcttg ccttctttag caatcgacgg agccataaag
    14401 atcgacagat acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt catcagatcg
    14461 accccggcta cgtgcgttga atttgcatgc ccgtaaatat caaactccag gccgacgttc
    14521 agagcgatga cgcccagacg acggatgatt tccgggttat tggaaatctc ctgcggacgc
    14581 aacacaatgc ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag ggaatcggcc
    14641 gagattgtca ggctggaggc gctggccccg ctgattttgc cggtttccag taaatgcacc
    14701 accgattcct gtagcacttc cgaatacatc atgaacggag gaatttccgg gttttccccc
    14761 agacgtgcca ttaccgcatt attgatattg cccacgccac tttgcagcgg cagaaattcc
    14821 ggcggaatac gcccatgcgc catttcctgc aataagaacg tgaccacgtt atcggcaatc
    14881 tgctggcaca tgggattttg cttatccagc atattaccgg cgtcgggcaa gttggtttcc
    14941 acgacggcga caatcttttt cggatcgatt tgcacatagc gggtaccgac gcgatccatt
    15001 gcatggaaga tcgacacgct attgcgccgc ggtggcgcgc caggaatcac aatatctgcc
    15061 agttctgcaa cgcgcggatc gtgatagtga ttaagttcaa tgatcacttt cttcgctcgc
    15121 agcagccagg tcggcgcatt accgatcccg ctggttaacc agactcgacc atccggtgcc
    15181 agtgccgatg cttcaatgac ggcaacatca atgtcgccga agaaaccgta attgaccatt
    15241 tgcgccactt cgctcaaatg caggtcaacg aaactcaccg cgccctgatt gatcttttta
    15301 cgtaaactgg acgatgtttg atatggcgca cgccaggaaa cagcatcggc gtcagaaagt
    15361 acatcgtcag cggcggcgct gattgacgcc cccgtcagca ggcgaatttg atacggcttt
    15421 ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg cgggtagggc tttcggcgaa
    15481 cccgctgggg taaagccgct aaatgccacc atgtcgttat gctggataat ttctgccgct
    15541 tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt cgcagggatt
    15601 aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcatccct tcctggtaat
    15661 cttcgctgtc ataaaccgcg cggcgcatcc cctgaatacg ttcaaattca tcggagttca
    15721 tggtgtgtgc ttcgcccagt acacgcagct cttctttgat aacggcaatg gctaacggcg
    15781 ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccacttcc acaacatggt
    15841 tgaggatgcc gacagccagc gcgcgctggg cggtgattgg cgaagcggta aaaatcagct
    15901 ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg aatgccgacc aggttatacg
    15961 ggacgccgag gtttacaggc gtcattgaga aggttgaggt actggcggcg atgatcagat
    16021 cggaactcat gatcatttca aatgcgccac cccaaacact accttccacc atcgaaatga
    16081 tcggtttcgg gaatttttgg atcatgcggg tgatttgacg caatggatca tcataggaga
    16141 gcggatcgcg accgccagac ggcagttcgt gaatatcgtg acctgcggag aagactttgg
    16201 atccacttgg cgcccgcaaa atgatacagc gaatttccgg ccggttgaga tcgcttaacg
    16261 cctgcataag atcatcaata aagactttac ttaaggcatt aagttttcgt ccatagttaa
    16321 actcaatgac cgccactttg ttgatagtgg caacgttcac atactgataa gacataaaaa
    16381 ttcctttaat caaaatattg cgtctggata aattcactga gctgccgcag gccggtgcgc
    16441 ggtgagagcg tattgttttt gaccgctaaa agcgtctggt ggtaatagcg atcgaaatct
    16501 tcattcgcga acaggtgatt cagtacttct tcttcggtct gcttacgcag ccattccacc
    16561 gattgttgtt gccgcacttg ttgtaaacga ccactggcag ttagcgcggt tttgaagtcg
    16621 atgatggcgt gccagatctc atcgattcca cgtttttcca gtgcgctaca agtcagaccc
    16681 cgtggctgcc attcgtcgta tttacgtcgc agaatatgca gggcactctc gtacatatgc
    16741 cgggcaatgg cgacattggt atggttatcg ccatcgtctt tgttgataac gagcagatca
    16801 gccacttcca tcagcccttt tttaatgccc tgcagatcat cgccgccacc ggcaatttgc
    16861 aacgagataa aacagtccac catgcgggcg acttccgttt ccgactgcgc gacgccaacc
    16921 gtttcgacaa tcactacgtc ataacccgcc gcttcgcata acagcattaa ttcccgtgct
    16981 cgctgactgg caccgcccag atgaccggag gagggtaccg ggcgaataaa cgccgcctcg
    17041 gcacgcgcca ggtcattcat gcgggtttta tccccgagaa tgctaccgcc agtgaccggg
    17101 ctgctgggat cgaccgcaat aacagcaacc tttaatccct cacgaatcaa caacatgcca
    17161 aaggcttcaa gaaaggtact tttccccgcg ccgggtgtgc cggtaacgcc cagtcgcagg
    17221 gcgttaccac agaacggcat aatagcgtca agcagctgtg tacttagcgc ctgatgacgt
    17281 gggtgacggc tttccaccag cgtcatggct tgggcgagcg tggcacgctc accctgacgt
    17341 aagcggcgaa tactttctgc cagcgtggct tcattaatca tgatgctggc ttatcagatt
    17401 cagtacgtcg cgcacactgt cgagcatagg tgtacctgga ccataaatcg ccgccacgcc
    17461 gcgctcttgc aggaaggcgt aatcctgcgg cggaatgacg ccacccgcga ccacgcagat
    17521 atcttcgcgt ccccattttt tcagcgcttc gaccagttcc gggatcagcg ttttatgacc
    17581 ggcagccagt gaggatgcgc ccactacgtg aacgtcgttt tctacggcca ggcgggcgat
    17641 ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg aaaccgagat cggaataggc
    17701 gctggcgatc actttcgcgc cgcgatcgtg tccatcctgg cccatcttag cgatcagaat
    17761 gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt tgcgcaacaa tggcatcgaa
    17821 ctcggaggcc gatttctcag actgatgata gctttgcgca atcacgccgg taacacactg
    17881 gcttggcacc agataacggt cgaaagcgac ttccagcgca tcggaaattt cacccagggt
    17941 ggcgcgaacg cgagcggcat taacagcggc agccagcagg ttttcgttat gctgtgcggc
    18001 gtgagtcagg gcgttcaacg cggcggttac ggcggcatca tcacgggtgg cgcgaatgcg
    18061 ttccagcgaa gcaatttgct cgttacgcac catcacgttg tcgatctcaa gtacatcggt
    18121 ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg atgacacgct tgccctggtc
    18181 gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt cgttttggca gacctgcttc
    18241 gatcgctttc gccatgccac cggcttcgtc gatctgttgg ataatagctc tggcttgttt
    18301 gacgatttga tcggtcagcg actcaatgta ataggatccg gccagtggat cgacggtgtg
    18361 gcagagttct gattcttcct ggatgatgat ctgggtgttg cgggcaatgc gtgctgagaa
    18421 atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta tgcagtgact gagtaccgcc
    18481 cagcgtcgca gccagtgctt caatggtggt gcggataacg ttgttatacg gatcctgttc
    18541 agtcaggctc cagcctgagg tctggcagtg ggtacgcagc gccagtgatt tcggatcctg
    18601 tgcgccaaat ccactgaccg cttcgctcca taaataacgt gccgcacgca acatggcgac
    18661 gttcataaac agatccatgc cgataccgaa gaataacgac aggcgaggag cgaagtcatc
    18721 aattttcagt ccggcagaga ttgctgcttt gatgtactca atcccatcgg cgatcgtaaa
    18781 tgctacctgc tgcacgcagt tggcacccgc ttcccccatg tggtaaccgc tgatactgat
    18841 ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg atgtcggcga taatgcgcat
    18901 tgacggtttt ggtgggtaaa tataggtgtt gcggcagagg tactctttga gaatatcgtt
    18961 ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc tcttctgcgg cgacgatata
    19021 aaacgccagt actggtagca ctgcgccatt catggtcatc gaaaccgaca ttttatccag
    19081 cgggatctgg tcgaacagga ctttcatatc ttccacggtg tcgatagcga cgcccgcttt
    19141 gccgacgtca cccgccacgc gtgggttatc ggagtcgtag ccacggtggg tggcaaggtc
    19201 aaacgcaacg gaaagacctt tttgcccggc ggccaggtta cggcgataaa aagcgttgga
    19261 ctcttttgct gttgaaaaac cagcatactg acggatggtc cacggttggg cggtatacat
    19321 agtggcacgc gggccacgaa cgtagggcgg caaaccagga agggtacctg tcacctccag
    19381 attatcgaga tcggcttcgg tatacagcgg cttgatggcg atcccttccg cggtttgctg
    19441 aaccagcgag tcgacagttt tctcccgacg gctcaattcc ttgttggcaa gctgttgcca
    19501 ctcctgctcg ttagacatta tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc
    19561 ttacggtgag ctttttacgt gggtttgtcg ccagcaaaaa aaatgcattc agacgtcgtt
    19621 ttttgccgcc tgagatttgt ctattttgtg atgtgattca aggaatacaa aaagaagtga
    19681 ggacaacgcc tgatatgtgc tatcaggcat tatttgatgg attaatcctg acgaaggact
    19741 ttgtgaccat aatcgagtaa cgcatcagtg actttacgca tcatgcggct ttcaggagca
    19801 aagcggtgcc agtagagcat ccgtcgttga aatagcccag gcgttaagtc aatcagttca
    19861 ccgctggcca gctctttctc gatttgcagg tgcgggatca tacagcaggt ggtgccctgg
    19921 cgagcaagtt gtacgaacgc ttctgaagaa ttaacgatat ggcagggtac gctgccagga
    19981 ggaagatcga agttttgctg caaaaaggcc tggtgcatat cgtcaagatg gtcaaacgcg
    20041 accactggcg ctttcaataa tgccgaacgc gttacgccgt taggaaaata tttttcggca
    20101 aacggttttg agctgacgaa cagatagtcg agcgcaccga gtttatcgac aagacaactc
    20161 ggcagcgcct gatgttgaat actcaccgcg ccgaccactt cgccgcggcg cagacgttcc
    20221 tgagtacggg tttcatcttc tacctgcaag ttgaggcgga taggcgaatc agccaacaca
    20281 ggagccagtg ccggaagcaa ccacgtcgcc agactgtcgg cgttgaccgc cagtgaaagc
    20341 agcagcggag tcgaaccggt ttgttcatcg cccagccact cttcttccag caactccacc
    20401 tggcgcagca gtgccagcag tttttgccct tgttccgtcg ggcgcggcgg tacggtacgc
    20461 accaacagcg gctgcccgaa catattttcc agttgcttaa tgcgctgtga gacggctgat
    20521 tgtgtaatgc acagcttttg tgccgcgcgc tcaaatcctc gttcacgtat caccgcatcc
    20581 agtgcctgta atgttctgta gtccgggcgt ttcattgctg ttgtctgctc ctggtaaatg
    20641 ctttttcctg cactatgaca caattttgtg tcaggttgca tataccgaag ccgtcggcgg
    20701 gtaacatttg tatcaatggt taaacgcgca gcttaaaaga tcagggattt gcgctattgc
    20761 atttcgctta gcgacttcgc gccctggtta tgttgaatgg cgtggcgtta ttgcctctgt
    20821 tcgcaccgtg gagcggcata cccagaccgt tgtatgcttt atttgcgggg cgaaagggga
    20881 tgcccgccat tgcgcgatat tttatggatg agttaaccac ccagcttgcc aacggggtct
    20941 gaatcgcttt ttttgtatat aatgcgtgtg aaatttcata ccacaggcga aacgatcatg
    21001 acgcaggatg aattgaaaaa agcagtagga tgggcggcac ttcagtatgt tcagcccggt
    21061 accattgttg gtgtaggtac aggttccacc gccgcacact ttattgacgc gctcggcacg
    21121 atgaaaggcc agattgaagg ggccgtttcc agttcagatg cttccactga aaaactgaaa
    21181 agcctcggca ttcacgtttt tgatctcaac gaagtcgaca gccttggcat ctacgttgat
    21241 ggcgcagatg aaatcaacgg ccacatgcaa atgatcaaag gcggcggcgc ggcgctgacc
    21301 cgtgaaaaaa tcattgcttc ggttgcggaa aaatttatct gtattgccga cgcctccaag
    21361 caggttgata ttctgggtaa attcccgctg ccagtagaag ttatcccgat ggcacgtagt
    21421 gcagtggcgc gccagctggt gaaactgggc ggtcgtccgg aataccgtca gggcgtggtg
    21481 accgataatg gcaacgtgat cctcgacgtc cacggcatgg aaatccttga cccgatagcg
    21541 atggaaaacg ccataaatgc gattcctggc gtggtgactg ttggcttgtt tgctaaccgt
    21601 ggcgcggacg ttgcgctgat tggcacacct gacggtgtca aaaccattgt gaaatgatct
    21661 gacgggggaa cctcccccgt taaaaaaatt ctcttcatta aatttggtga catgtgtcac
    21721 gcttttacca ggcaattgtc gattgctcta aataaatcct ctaaaccagc atattcatcc
    21781 aagaattacc tttgcgtgat atttcctcaa catcgcgacg caaacgttca tattgccgca
    21841 atattatttt ttgatatgtt gaaaggcgga tgcaaatccg cacacaacat ttcaaaagac
    21901 aggattgggt aaatggcaaa ggtatcgctg gagaaagaca agattaagtt tctgctggta
    21961 gaaggcgtgc accaaaaggc gctggaaagc cttcgtgcag ctggttacac caacatcgaa
    22021 tttcacaaag gcgcgctgga tgatgaacaa ttaaaagaat ccatccgcga tgcccacttc
    22081 atcggcctgc gatcccgtac ccatctgact gaagacgtga tcaacgccgc agaaaaactg
    22141 gtcgctattg gctgtttctg tatcggaaca aaccaggttg atctggatgc ggcggcaaag
    22201 cgcgggatcc cggtatttaa cgcaccgttc tcaaatacgc gctctgttgc ggagctggtg
    22261 attggcgaac tgctgctgct attgcgcggc gtgccggaag ccaatgctaa agcgcaccgt
    22321 ggcgtgtgga acaaactggc ggcgggttct tttgaagcgc gcggcaaaaa gctgggtatc
    22381 atcggctacg gtcatattgg tacgcaattg ggcattctgg ctgaatcgct gggaatgtat
    22441 gtttactttt atgatattga aaacaaactg ccgctgggca acgccactca ggtacagcat
    22501 ctttctgacc tgctgaatat gagcgatgtg gtgagtctgc atgtaccaga gaatccgtcc
    22561 accaaaaata tgatgggcgc gaaagagatt tcgctaatga agcccggctc gctgctgatt
    22621 aatgcctcgc gcggtactgt ggtggatatt ccggcgctgt gtgatgcgct ggcgagcaaa
    22681 catctggcgg gggcggcaat cgacgtattc ccgacggaac cggcgaccaa tagcgatcca
    22741 tttacctctc cactgtgtga attcgacaac gtccttctga cgccacacat tggcggttcg
    22801 actcaggaag cgcaggagaa tatcggcttg gaagttgcgg gtaaattgat caagtattct
    22861 gacaatggct caacgctctc tgcggtgaac ttcccggaag tctcgctgcc actgcatggt
    22921 ggtcgtcgtc tgatgcacat ccacgaaaac cgtccgggcg tgctaactgc gctgaacaaa
    22981 atcttcgccg agcagggcgt caacatcgcc gcgcaatatc tacaaacttc cgcccagatg
    23041 ggttatgtag ttattgatat tgaagctgac gaagacgttg ccgaaaaagc gctgcaggca
    23101 atgaaagcta ttccgggtac cattcgcgcc cgtctgctgt actaattccc cttctctgaa
    23161 aattaacggg caggtcactg acttgcccgt ttttttatcc cttctccaca ccgttgccgc
    23221 gttgcgcatt ctactttgcg agtctgctcc agaaaataaa tcaaagtcag atcaatgcgt
    23281 tgtgtttatt taatggggat ttacgatggc gggacagcat ggggctgtaa cggtaaagcc
    23341 ctcaccgaag cgagggctta aaggagaagg gttatgatgc gacttgtcat catactgatt
    23401 gtactgttac tgataagttt cggagcttat taacagtcag tctcaaggga ggagtcatcc
    23461 tcccttaccc ttactcacta aattaggtca aagaatcaac gatgtcaatc agggcgatgc
    23521 ggggtgcatc gccctgaacc gaactttttg atagccactt catcgcccac gtttactgcc
    23581 gtaatagatc ctttttccca aaaattatct ctgttttgca tggggttttt cgagcacgct
    23641 cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga gcttacgata
    23701 gctgggcagc atggggctgt aacggtaaag ccctcaacga agcgagggct tgaaggagaa
    23761 gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt ttcccagctt
    23821 attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac taaattaggt
    23881 taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc actcccagac
    23941 tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg ggagtttttc
    24001 caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct gccagttttg
    24061 taaggtccga tcataaaaac caccgcccat tcccaggcgc tgaccgtact catcaaaggc
    24121 gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac gcacatccag
    24181 ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat ggtaattcag
    24241 gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc gcttaccggc
    24301 gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag agaggaatac
    24361 agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag cggcttgttg
    24421 acccatttcc agctgttgtt ccggcgttaa cgcacgacga cgttgccgaa tcattttgcg
    24481 gatttcttgt cgggataatg tcagtgggag ctctggtagt tgcgtcatgg ctggtgccag
    24541 ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc ttggttcaag
    24601 gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacgaac cgagcatgct caccaaccgc
    24661 ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg cttgcgaaca
    24721 tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa gtgtcattca
    24781 aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg ttctatggtc
    24841 tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt ctttgctttt
    24901 tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg ttcagtattt
    24961 gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc cgctgcctga
    25021 ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc aaaaatttgg
    25081 atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct gccttcgtct
    25141 ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa gagacaagcc
    25201 cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct ccctttgacg
    25261 acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga accagtatct
    25321 gaaccaacaa gggacgggtc tgacccctgc cgagatgcat ggtttaatca gtgggatgat
    25381 atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga cgaacgaagg
    25441 catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg ccaccagcga
    25501 tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg atgatgtcag
    25561 cgttttcgat cgggctgatg cactggcagg ttgggtcaat cacttcctgc ttggtcttgg
    25621 cgttacgcaa ccgaagctgg acaaagtgac cggcgaaacc ggtgaagcca tcgacgatct
    25681 gcgtaacatc gcgcagctgg gctacgacga agacgaagat caggaagagc ttgaaatgtc
    25741 gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg acacctttac
    25801 tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa aacaaaaaac
    25861 gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc cgtcaggccc
    25921 tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca gaagtaacac
    25981 gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac ttcaccggct
    26041 ttaacgaacc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat aaccacagcg
    26101 ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt cgcttaggcc
    26161 aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc gaaatcaatc
    26221 agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag ggcgaatatg
    26281 catatgctga tgaaatcgtg aacagtgcgc tggaaaaact gcgtaaaggt tcgcggcaaa
    26341 atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa atgcgcctgt
    26401 tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc gccatggcac
    26461 atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg gaaggcgaaa
    26521 ttcaccacga atttaaccgc cacggtgcgc gctatccttc ctataacacc attgtaggca
    26581 gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg cgcgacggtg
    26641 acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat attacccgca
    26701 ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac attgtgctgg
    26761 agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg gaagtcactg
    26821 gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg aaaggtaatg
    26881 ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc cttagccact
    26941 ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg cgcattctgg
    27001 aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat gcggatgtgc
    27061 cagaacaata tcgcggtatc ggcattcgca ttgaagacga cattgtgatt accgaaaccg
    27121 gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa gcgttgatgg
    27181 ctgctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg gcgcgacgct
    27241 ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga ttgaagcgac
    27301 tgcgccagag tcacatgctc atccgggctt tgatggacga gcgatagcgc tggcggcggg
    27361 tacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggctgatt gcgcaaccgc
    27421 catcactacc gtgcatgtca gcgatcgtgg tcatgctgga ttcgtcaccc tcgccgcaga
    27481 agattaccaa ctggcggcgc tggggcaggt tgtcgaattg cataatgtcg ggcaacggct
    27541 gtttgcattg ctgcgaaaag cacctggcgt aatgctgcat tgccccgatc gcgtagctaa
    27601 cgttgcccgt actcagagtc atgttgaagt gacgctggag agcggcgaga tgctgacggg
    27661 ccgcgtgctg atagccgctg atggcaccca ttcagcgtta gccaccgcgt gcggcgttga
    27721 ttggcagcag gagccttacg aacaactggc agtgattgcc aacgttgcta cttctgttgc
    27781 gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga tgttgccgat
    27841 gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg aagaggtgct
    27901 gtcgtggagc gacgagaagt tttgccgtga actccagtct gcctttggct ggcgactggg
    27961 gcaaattacc cacgccggta aacgcagtgc ttatccgctg gcattaaccc gcgccgccag
    28021 accgattacc catcgcactg tgctggtggg caatgcggcg caaactctgc acccgattgc
    28081 cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa ccctgactca
    28141 ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct atcagcagcg
    28201 tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac atctttttgc
    28261 caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga tggaattatt
    28321 caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc gttgaaggcg
    28381 gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggcggc ggcatggtgg
    28441 ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta ctggagcagc
    28501 gcgtaccgga acctctggcg gtagatgcac taccacaact gcgcgtttcg gctatcaatg
    28561 ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc gctcgtaggg
    28621 ccagttgtta tcacggcatg gaagtgtggg acaaagacag ttttggtcac atctcgtttg
    28681 acgatcaaag catgggctat agccatcttg ggcacatcgt tgaaaactca gtgattcact
    28741 acgcgctgtg gaacaaagcg cagcagtcgt cagatattac tctgttggcc cccacagaat
    28801 tacagcaggt cgcctgggga gaaaatgaaa ccttcctgac gctgaaagat ggcagcatgt
    28861 taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc aacaaagcag
    28921 atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc attcgcacgg
    28981 aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt ctggcctttt
    29041 taccgcttag cgatccgcat ctttgctcga ttgtctggtc actgtcgcca gaggaagcgc
    29101 agcggatgca gcaggcaagt gaagacgaat ttaatcgcgc gttaaatatc gcttttgata
    29161 atcgccttgg cttatgcaag gttgagagcg cgcgtcaggt gttcccgctg acggggcgtt
    29221 atgcgcgcca gtttgccgca caccgtctgg cgctggtggg cgacgccgcg cataccattc
    29281 acccactggc ggggcagggc gttaatctcg gatttatgga cgccgcagag ctgattgctg
    29341 aactgaaacg gttgcatcgt cagggtaaag acatcgggca gtacatttac ctgcgccgct
    29401 atgaacgtag ccgcaagcac agcgcggcgc tgatgctggc tggtatgcag ggttttcgcg
    29461 atctcttttc cggcgctaat ccggcgaaaa aactgctgcg tgatattggt ttgaaactgg
    29521 ccgacacgct tcctggcgtt aagccgcaac ttatccgcca ggcaatggga ttaaacgatt
    29581 tgcctgaatg gctgcgttaa aaatttctcc tctgttgttt atttgatacc catcacactt
    29641 tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta atttcacctc
    29701 cgttttcgca ttatattttc taataccatg attttttgat ttagtgtttt ttgacatttt
    29761 tttagctctt aatattgtct tattcaaatt gactttctca tcacatcatc tttgtataga
    29821 aattggtgta ttttttggtt ttttattctg tcgcgatttt tgcatttttt aaccataagc
    29881 taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta agttcaggca
    29941 aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttt gtggtgataa
    30001 tttcaccatg aaaaagttgt cagccccgct tattcaatga ggacaagatg gcacaacaga
    30061 ctcctttgta cgaacaacac acgctttgcg gcgctcgcat ggtggatttc cacggctgga
    30121 tgatgccgct gcattacggt tcgcaaatcg acgaacatca tgcggtacgt accgatgccg
    30181 gaatgtttga tgtgtcgcat atgaccatcg tcgatctccg cggcagccgc acccgggagt
    30241 ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac caaaagaggc aaagcccttt
    30301 actcggggat gttgaatgcc tctggcggtg tgatagatga tctcatcgtc tactacttta
    30361 ctgaagattt cttccgcctc gttgttaact ccgccacccg cgaaaaagac ctctcctgga
    30421 ttacccaaca cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac ctttccatga
    30481 ttgccgtgca agggccgaat gctcaggcaa aagctgccac actgtttaat gatgctcagc
    30541 gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca ggtgggcgat ctgtttattg
    30601 ccaccactgg ttataccggt gaagcgggct atgaaattgc gctgcccaat gaaaaagcgg
    30661 ccgatttctg gcgtgcgctg gtggaagcgg gcgttaagcc ctgcggtctg ggggcgcgtg
    30721 acacgctgcg tctggaagcg ggcatgaatc tttatggtca ggagatggac gaaactattt
    30781 ctcctttagc cgccaacatg ggctggacta tcgcctggga accgacagat cgtgacttta
    30841 tcggtcgtga agccctggaa gtgcagcgtg agcatggtac agaaaaactg gttggtctgg
    30901 tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt acgctttacc gatgcgcagg
    30961 gcaaccagca tgaaggcatt atcaccagcg gtactttctc cccgacgctg ggttacagca
    31021 ttgcgctggc gcgcgtgccg gaaggtattg gcgaaacggc gattgtgcaa attcgcaacc
    31081 gtgaaatgcc ggttaaagtg acaaaacctg tttttgtgcg taacggcaaa gccgtcgcgt
    31141 gatttacttt tttggagatt gattgatgag caacgtacca gcagaactga aatacagcaa
    31201 agaacacgaa tggctgcgta aagaagccga cggcacttac accgttggta ttaccgaaca
    31261 tgctcaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg gcgcaacggt
    31321 tagcgcgggc gatgactgcg cggttgccga atcggtaaaa gcggcgtcag acatttatgc
    31381 gccagtaagc ggtgaaatcg tggcagtaaa cgacgcactg agcgattccc cggaactggt
    31441 gaacagcgaa ccgtatgcag gcggctggat ctttaaaatc aaagccagcg atgaaagcga
    31501 actggaatca ctgctggatg cgaccgcata cgaagcattg ttagaagatg agtaacggct
    31561 ttattcctct tctgcgggag aggatcaggg tgaggaaaat ttatgcctca ccctcactct
    31621 cttcgtaagg agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac
    31681 agacgttaag ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg
    31741 ccgcgcaaca gcaagaaatg ctgaatgccg ttggtgcaca atcgttaaac gcgctgaccg
    31801 gccagattgt gccgaaagat attcagcttg cgacaccacc gcaggttggc gcaccggcga
    31861 ccgaatacgc cgcactggca gaactcaagg ctattgccag tcgcaataaa cgcttcacgt
    31921 cttacatcgg catgggttac accgccgtgc agctaccgcc ggttatcctg cgtaacatgc
    31981 tggaaaatcc gggctggtat accgcgtaca ctccgtatca gcctgaagtc tcccagggcc
    32041 gccttgaagc actgctcaac ttccagcagg taacgctgga tttgactgga ctggatatgg
    32101 cctctgcttc tcttctggac gaggccaccg ctgccgccga agcaatggcg atggcgaaac
    32161 gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc ttccaatgtg catccgcaaa
    32221 cgctggatgt ggtccgtact cgtgctgaaa cctttggttt tgaagtgatt gtcgatgacg
    32281 cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct gttacagcag gtaggcacta
    32341 ccggtgaaat tcacgactac actgcgctta ttagcgaact gaaatcacgc aaaattgtgg
    32401 tcagcgttgc cgccgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg
    32461 cggatattgt ttttggttcg gcgcaacgct tcggcgtgcc gatgggctac ggtggcccac
    32521 acgcggcatt ctttgcggcg aaagatgaat acaaacgctc aatgccgggc cgtattatcg
    32581 gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc
    32641 aacatattcg tcgtgagaaa gcgaactcca acatttgtac ttcccaggta ctgctggcaa
    32701 acatcgccag cctgtatgcc gtttatcacg gcccggttgg cctgaaacgt atcgctaacc
    32761 gcattcaccg tctgaccgat attctggcgg cgggcctgca acaaaaaggt ctgaaactgc
    32821 gccatgcgca ctatttcgac accttgtgtg tggaagtggc cgacaaagcg ggcgtactga
    32881 cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcc gtggggatca
    32941 cccttgatga aaccaccacg cgtgaaaacg taatgcagct tttcagtgtg ctgctgggcg
    33001 ataaccacgg gctggagatc gacacgctgg acaaagacgt ggctcacgac agccgctcta
    33061 tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctatc
    33121 acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga
    33181 atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga
    33241 tcccaatcac ttggacggaa tttgccgaac tgcacccgtt ttgcccgccg gaacaggctg
    33301 aaggttatca gcagatgatt gcgcagctgg ctgactggct ggtgaaactg accggttacg
    33361 acgccgtttg tatgcagccg aactccggcg cacagggcga atacgcgggc ctgctggcga
    33421 ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt
    33481 ctgcgcacgg aactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg
    33541 cgtgtgataa aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg
    33601 gcgataacct ctcctgtatc atggtgactt atccttctac ccacggcgtg tatgaagaaa
    33661 cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg
    33721 cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt tattggcgcg gacgtttcgc
    33781 acctcaacct gcataaaact ttctgcattc cgcacggcgg tggtggtccg ggtatgggac
    33841 cgatcggtgt gaaagcgcat ctggcaccgt ttgtaccggg tcatagcgtg gtgcaaatcg
    33901 aaggcatgtt aacccgtcag ggcgcagttt ctgcggcacc gttcggtagc gcctctatcc
    33961 tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc
    34021 aggtggcaat cctcaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc
    34081 tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga
    34141 aagaagaaac cggcatcagc gagctggata ttgccaagcg cctgatcgac tacggtttcc
    34201 acgcgccgac gatgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg
    34261 aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaattg
    34321 accaggtgaa agccggtgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca
    34381 ttcagagcga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcggtattcc
    34441 cggcaggcgt ggcagacaaa tactggccga cggtgaaacg tctggatgat gtttacggcg
    34501 accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga
    34561 gtattttcta aaggcgcttc ggcgcctttt tagtcagatg acaaagtaca aaagtgctca
    34621 gacagtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc cggcactggc
    34681 gcgaatattt accctcaccc cgaccctctc cctgaaaggg cgagggggaa aagcgtgcca
    34741 acattgaaga ttgagccagt ttgttagcaa tctcaaagat acgtcaacga attaattttt
    34801 ctcggaaaaa caaatggcta tagcacttgt gaccggtggc agtcgcggca tcgggcgggc
    34861 aactgcatta ctgttggcgc aagaagggta tacggtggcg gttaattatc agcaaaacct
    34921 ccacgcggcg caggaagtga tgaacttaat aatgcaagcc ggtggcaaag cattcgtgct
    34981 ccaggcggat atcagcgacg aaaaccaggt cgttgcgatg tttacagcaa tcgatcagca
    35041 cgatgaaccg ctagcagcgc tggtcaataa cgccgggatc ttgtttaccc agtgcaccgt
    35101 tgaaaacctt accgcagagc gaatcaaccg agtactttcc accaacgtga cgggatattt
    35161 tctctgctgc cgcgaggcgg taaaacgcat ggcgcttaaa aatggtggca gtggcggcgc
    35221 tatcgtcaat gtctcttcgg tggcctcacg gttgggttcg ccaggggaat atgttgatta
    35281 cgcggcatcg aaaggggcga ttgattcgtt aaccaccgga ctatcgctgg aagtcgccgc
    35341 gcaggggatc cgcgttaact gcgtgcggcc agggtttatt tataccgaaa tgcacgccag
    35401 cgcggcgagc ctggacgcgt cgatcgcgtt aagtcgaaca tccccatgca gcgtggtgga
    35461 caggcagaag aggtcgcgca ggccattgtc tggctactaa gtgataaagc ctcttacgtc
    35521 acgggaagtt ttatcgattt ggcgggcggg aaataaaaca gggaagttgt ctgaccggat
    35581 gcaacaagta ttgcatccgg tacttcatcg acttaaagct tctcgccgtt gctggcaatc
    35641 acctctttgt accagttaaa gctcttctta cgtgaacgcg acatatcgcc agtaccgtcg
    35701 tcatgtttat tcacatagat aaagccgtag cgtttgctgt actgcccggt agtgaatgac
    35761 acgcagtcga tgcagcccca cggtgtgtag cccatcagat ccacaccatc gtaagtcact
    35821 gcttttttca tctcttcaat atgggcgcgc aggtagtcaa tgcggtagtc gtcgttgatg
    35881 ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat aaacagcggc
    35941 ctctgataac gctcatacag ttcgcaaagg gcatagcgca gacctactgg atcaatctgc
    36001 cagccccagt cagacgcttt aacatacggg ttcggtacgc tgccttcaaa accagagatc
    36061 gcatcgccgg tgccgccttc ggccttcact gcgttggtca tgtaatagct gaaaccaaga
    36121 taatcgcagg tgccttcgcg cagtacatcg agatcgccgt cttccatttt gatattaaat
    36181 ccgcgacgtt cccactcgtt caatacatag gacgggtaat agccgcatag ctgcacatcg
    36241 gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca tcacatcgtc cgggttacat
    36301 gagtagggat agagcggcac catcgccagc atacagccga ctttcatctc cgggttaatg
    36361 cgacgcgcgg ctttcactgc cagggcgctg gcgacaaact ggtgatgcag cacctggtac
    36421 atcgtctctt ccgggttgtc atgctcggta tacaccacgc cggagcagca gtaaccaaac
    36481 agcggcgcac gccagttacg ctggttgttt atttcgttga aggtcatcca gtatttgact
    36541 ttatgcttgt agcgttcgaa taccacttcc gcgaaacgga caaagaaatc aacgaattta
    36601 cggttggtcc agctaccgta ttgctgcacc agatgcagcg gcatttcaaa gtgggagagg
    36661 gttatcaccg gttcgatgtt gtatttcagc agttcatcga acagagagtc gtaaaacttc
    36721 agcccttctt cgtttggctg agcttcatca ccttttggaa aaatacgcgt ccaggcgatg
    36781 gaagtccgaa aacatttgaa gcccatttcg gcaaatagct tgatgtcttc cttgtagtga
    36841 ccataaaaat caacggcttc atggtttgga tagtattttc ctggcaagac ttctttggtg
    36901 atttcgcgcg gcacgccgtg tgcgccaccg gtcagaacgt cacaaatgct cggtcctttg
    36961 ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa cggcgccacc ccataagaaa
    37021 tctttcggta aggtgagttt tttcactatc ataggctcca gatcagtcaa ttcttacagg
    37081 aaatagtacc atgcagccaa acgacatcac ttttttcaac gtttccagga tgacattctg
    37141 gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat
    37201 gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga agtcaccgca
    37261 acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga aaatatgacc
    37321 ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca attttatatg
    37381 attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat
    37441 ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac
    37501 tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt ctctggagta
    37561 agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa
    37621 cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt tgctactggt
    37681 tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg
    37741 cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc
    37801 aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta
    37861 cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat
    37921 ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc gattcaaaat
    37981 tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat
    38041 ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc
    38101 gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg
    38161 cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc
    38221 gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca ggcatcggag
    38281 cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg
    38341 ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg
    38401 cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg
    38461 tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
    38521 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac
    38581 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctgacac
    38641 tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
    38701 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    38761 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa ccagcttcaa
    38821 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    38881 tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    38941 accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc
    39001 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    39061 cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct
    39121 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
    39181 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
    39241 ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
    39301 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    39361 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    39421 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    39481 gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa ttgttgtgaa
    39541 ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    39601 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    39661 gcccgaattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    39721 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    39781 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
    39841 gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    39901 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    39961 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    40021 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta atgctcgcca
    40081 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    40141 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    40201 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    40261 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    40321 taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
    40381 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
    40441 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    40501 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    40561 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaataattt
    40621 taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcctggattt
    40681 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    40741 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    40801 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    40861 cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca
    40921 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    40981 gctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    41041 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    41101 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    41161 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    41221 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    41281 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    41341 gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    41401 taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    41461 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggggaaga
    41521 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    41581 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    41641 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    41701 tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    41761 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    41821 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    41881 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    41941 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    42001 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    42061 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    42121 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    42181 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    42241 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    42301 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    42361 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    42421 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    42481 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    42541 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    42601 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    42661 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    42721 caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag cggtgtcgaa
    42781 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    42841 gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
    42901 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    42961 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    43021 ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc ggttattgtt
    43081 accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    43141 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    43201 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    43261 attcctaacc tggcagaact gctggatctg gtagcactgg ggacagtagc ggacgtcgtg
    43321 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    43381 aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc acaaaaacta
    43441 gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg gcgactggat
    43501 gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggcgaagc gcgcgtgctg
    43561 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaggtt
    43621 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    43681 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgtatcaaa
    43741 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    43801 tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt agacacactc
    43861 taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    43921 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggcc
    43981 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    44041 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    44101 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    44161 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    44221 accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa gctcgatatc
    44281 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    44341 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    44401 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    44461 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    44521 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    44581 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    44641 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    44701 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    44761 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgttacct
    44821 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
    44881 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    44941 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg
    45001 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    45061 cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg atgatgatat
    45121 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    45181 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat
    45241 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg ccatgaagca
    45301 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    45361 ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    45421 ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
    45481 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    45541 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    45601 gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga acgatttccg
    45661 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    45721 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    45781 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    45841 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    45901 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    45961 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    46021 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    46081 taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    46141 cggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    46201 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    46261 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    46321 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    46381 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    46441 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    46501 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    46561 catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    46621 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    46681 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc
    46741 gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg
    46801 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct
    46861 ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta
    46921 cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg
    46981 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    47041 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    47101 atttaagctg ggtaaatgca gataatcgta ttctggcttc gcgatttgtc gcctgcatca
    47161 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    47221 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    47281 cggcaaatac cggacacact tcattttcca caatgcctct cggatcggtg gcgcggtagc
    47341 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    47401 ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa accgagttag
    47461 tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgcccct
    47521 tggcattaaa cagccaactg gagaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    47581 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    47641 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    47701 aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt
    47761 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    47821 acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga atatagcgcg
    47881 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    47941 aca
//
LOCUS       DAECNO010000020        47295 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.020, whole genome shotgun sequence.
ACCESSION   DAECNO010000020 DAECNO010000000
VERSION     DAECNO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.020"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="KKO08_001143"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="KKO08_001143"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBD6317471.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KKO08_001144"
     CDS             complement(1301..2077)
                     /gene="sseB"
                     /locus_tag="KKO08_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBD6317472.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KKO08_001145"
     CDS             complement(2219..3502)
                     /gene="pepB"
                     /locus_tag="KKO08_001145"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBD6317473.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KKO08_001146"
     CDS             complement(3561..3761)
                     /gene="iscX"
                     /locus_tag="KKO08_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBD6317474.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KKO08_001147"
     CDS             complement(3773..4108)
                     /gene="fdx"
                     /locus_tag="KKO08_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBD6317475.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KKO08_001148"
     CDS             complement(4110..5960)
                     /gene="hscA"
                     /locus_tag="KKO08_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBD6317476.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KKO08_001149"
     CDS             complement(5977..6492)
                     /gene="hscB"
                     /locus_tag="KKO08_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBD6317477.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KKO08_001150"
     CDS             complement(6588..6911)
                     /gene="iscA"
                     /locus_tag="KKO08_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBD6317478.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KKO08_001151"
     CDS             complement(6928..7314)
                     /gene="iscU"
                     /locus_tag="KKO08_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBD6317479.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KKO08_001152"
     CDS             complement(7342..8556)
                     /gene="iscS"
                     /locus_tag="KKO08_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBD6317480.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KKO08_001153"
     CDS             complement(8668..9156)
                     /gene="iscR"
                     /locus_tag="KKO08_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBD6317481.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KKO08_001154"
     CDS             complement(9426..10166)
                     /gene="trmJ"
                     /locus_tag="KKO08_001154"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBD6317482.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10285..11088
                     /gene="suhB"
                     /locus_tag="KKO08_001155"
     CDS             10285..11088
                     /gene="suhB"
                     /locus_tag="KKO08_001155"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBD6317483.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11233..12087
                     /locus_tag="KKO08_001156"
     CDS             11233..12087
                     /locus_tag="KKO08_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD6317484.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12278..13558
                     /gene="csiE"
                     /locus_tag="KKO08_001157"
     CDS             12278..13558
                     /gene="csiE"
                     /locus_tag="KKO08_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBD6317485.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13550..14689)
                     /locus_tag="KKO08_001158"
     CDS             complement(13550..14689)
                     /locus_tag="KKO08_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBD6317486.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KKO08_001159"
     CDS             complement(14849..15739)
                     /gene="hcaR"
                     /locus_tag="KKO08_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBD6317487.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15875..17236
                     /locus_tag="KKO08_001160"
     CDS             15875..17236
                     /locus_tag="KKO08_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBD6317488.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17233..17751
                     /locus_tag="KKO08_001161"
     CDS             17233..17751
                     /locus_tag="KKO08_001161"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBD6317489.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17751..18071
                     /locus_tag="KKO08_001162"
     CDS             17751..18071
                     /locus_tag="KKO08_001162"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBD6317490.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18068..18880
                     /gene="hcaB"
                     /locus_tag="KKO08_001163"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18068..18880
                     /gene="hcaB"
                     /locus_tag="KKO08_001163"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBD6317491.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18890..20092
                     /locus_tag="KKO08_001164"
     CDS             18890..20092
                     /locus_tag="KKO08_001164"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBD6317492.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20189..20611
                     /locus_tag="KKO08_001165"
     CDS             20189..20611
                     /locus_tag="KKO08_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD6317493.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20659..21531)
                     /locus_tag="KKO08_001166"
     CDS             complement(20659..21531)
                     /locus_tag="KKO08_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBD6317494.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21543..22637)
                     /locus_tag="KKO08_001167"
     CDS             complement(21543..22637)
                     /locus_tag="KKO08_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD6317495.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22670..23668)
                     /locus_tag="KKO08_001168"
     CDS             complement(22670..23668)
                     /locus_tag="KKO08_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6317496.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23693..25204)
                     /locus_tag="KKO08_001169"
     CDS             complement(23693..25204)
                     /locus_tag="KKO08_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD6317497.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25227..26210)
                     /locus_tag="KKO08_001170"
     CDS             complement(25227..26210)
                     /locus_tag="KKO08_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBD6317498.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26307..29408)
                     /locus_tag="KKO08_001171"
     CDS             complement(26307..29408)
                     /locus_tag="KKO08_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBD6317499.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29415..29588)
                     /locus_tag="KKO08_001172"
     CDS             complement(29415..29588)
                     /locus_tag="KKO08_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317500.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29706..30899
                     /locus_tag="KKO08_001173"
     CDS             29706..30899
                     /locus_tag="KKO08_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBD6317501.1"
                     /translation="MRACINNQQILHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KKO08_001174"
     CDS             complement(31097..32350)
                     /gene="glyA"
                     /locus_tag="KKO08_001174"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBD6317502.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32677..33867
                     /gene="hmpA"
                     /locus_tag="KKO08_001175"
     CDS             32677..33867
                     /gene="hmpA"
                     /locus_tag="KKO08_001175"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBD6317503.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KKO08_001176"
     CDS             complement(33912..34250)
                     /gene="glnB"
                     /locus_tag="KKO08_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBD6317504.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KKO08_001177"
     CDS             complement(34311..35645)
                     /gene="glrR"
                     /locus_tag="KKO08_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBD6317505.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KKO08_001178"
     CDS             complement(35635..36348)
                     /gene="qseG"
                     /locus_tag="KKO08_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBD6317506.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KKO08_001179"
     CDS             complement(36513..37940)
                     /gene="qseE"
                     /locus_tag="KKO08_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBD6317507.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KKO08_001180"
                     /gene_synonym="purI"
     CDS             complement(38516..42403)
                     /gene="purL"
                     /locus_tag="KKO08_001180"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBD6317508.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42662..44218
                     /gene="mltF"
                     /locus_tag="KKO08_001181"
     CDS             42662..44218
                     /gene="mltF"
                     /locus_tag="KKO08_001181"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBD6317509.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KKO08_001182"
     CDS             complement(44215..44751)
                     /gene="tadA"
                     /locus_tag="KKO08_001182"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBD6317510.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KKO08_001183"
     CDS             complement(44776..45411)
                     /gene="yfhb"
                     /locus_tag="KKO08_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBD6317511.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45620..46468
                     /locus_tag="KKO08_001184"
     CDS             45620..46468
                     /locus_tag="KKO08_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBD6317512.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46807..47295)
                     /locus_tag="KKO08_001185"
                     /pseudo
     CDS             complement(46807..47295)
                     /locus_tag="KKO08_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11278 a        12955 c        11891 g        11171 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtattttaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta cacatgagca acgcaggtgc ttcacagcaa
      601 aacaatactc accagtaact ctctttttgt caagcaaaag agagtaatta ttgtttattt
      661 agcgtattat cgacaccggc cctttccgcc gtgttcggta ataaaataac ctggcttatt
      721 agtccgaatt cagacaaata taaacaaatc ctgctcaaaa ttaaaaattc taaccggtaa
      781 aagatattac ttaaacatgt aaatttactt tcctttaaaa aacaaaaaac cgccaaaatc
      841 aggcggttat ttgttgctgg tccggttcgc ggcctttcca gcaggttgta ttaccgtagt
      901 aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa
      961 agagagttat tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca
     1021 agcgggcgct ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg
     1081 cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc
     1141 acaatgaaga tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa
     1201 gtaaataata accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc
     1261 agcaagtaag cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt
     1321 taaagtcacg caaaaaacca ccccagcgac gttcatagaa tggcgcaata tgttcggtaa
     1381 taaagtggct aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc
     1441 caggtaatgt atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat
     1501 cggcttcaat gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa
     1561 taaaagcacg cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt
     1621 gtgctggcgg ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct
     1681 ggctgctcag cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt
     1741 ctttaccggt cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca
     1801 atgtgcgaac gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt
     1861 ctaacgaggt gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat
     1921 caagcgcact atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg
     1981 tggattccag tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt
     2041 ccagcaggtc ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt
     2101 tcggggcgca aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt
     2161 tgaatttgca gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt
     2221 acgccgttaa cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact
     2281 gttcaaccgg cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat
     2341 agttctcaac aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg
     2401 cgcttccggt attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg
     2461 ccagcggcag acgccagaat ggttcgttct cttgtgaggc actcgccagt aaacgaccgg
     2521 caagcgcatc gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg
     2581 ccccggtgag ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca
     2641 gaccatcggc aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac
     2701 cgttgcgata ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg
     2761 cacagcagag gaacagcttc acgcgcttgt tcagtccgcg cataatggca aatgccagcg
     2821 ccccggtaac cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct
     2881 gtttgatgct gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca
     2941 ccggtgcttc tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt
     3001 ctgaaccgcg cccgacagtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt
     3061 tggtgatccg ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct
     3121 gtgccagttg cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt
     3181 cgatgatcat caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact
     3241 ccactttacg cgtgcctttc gggcctttat aaccttgcca gaatgcccag cagcgatccg
     3301 catcccagcc ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcggg
     3361 ccgcacgctg gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat
     3421 tattaatgct gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg
     3481 taatcttcat cgcttctgtc attttagtta tccttattcg tttaacagca aaacgggcta
     3541 cccccaggca gcccgttcat tcactcggct tcgtccagcc agactaacaa aatcgcttcg
     3601 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
     3661 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
     3721 gcttcgccaa tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct
     3781 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
     3841 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
     3901 cttcctgctc tgagctttcc ggcagtgagt caaaaccttc acgaacgatg cagtggcagg
     3961 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc aataccgtta cgcagcgctg
     4021 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagaggtcct
     4081 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
     4141 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
     4201 tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct gcgccacttc actcaggtga
     4261 gcggcagcat tgtcaatgac ctgacgctct gcggcgctta acagcgcggc atcagcagcc
     4321 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcata
     4381 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
     4441 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
     4501 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
     4561 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
     4621 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
     4681 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
     4741 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc
     4801 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
     4861 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
     4921 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
     4981 tcagcttcta cacccgcgtc tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc
     5041 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
     5101 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggt ggcatccagc
     5161 agttcacgct gaacgcggtt atcgctacga tcaggaatgt ccgcctgctc gcgaatgtaa
     5221 tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc
     5281 acttcaaaca cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg
     5341 tcataaacag cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagca
     5401 gtcggttcgt taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg
     5461 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
     5521 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
     5581 accgggttca gtagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
     5641 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
     5701 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
     5761 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
     5821 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
     5881 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
     5941 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
     6001 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca ccgtatccgc
     6061 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
     6121 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
     6181 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
     6241 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
     6301 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
     6361 ttgttccgcc aggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
     6421 aaaacgcagg ctcagtgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
     6481 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
     6541 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
     6601 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
     6661 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
     6721 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
     6781 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
     6841 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
     6901 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
     6961 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
     7021 ttcaagttct tcagcaatat cggtgttttt gatcgcctgc gcttcgtcga gagacttccc
     7081 tttcacccat tcggtgacca gggagctgga agcgatagcg gaaccgcagc cgtaagtttt
     7141 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcattacgtc
     7201 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tatcaaagga
     7261 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
     7321 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
     7381 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
     7441 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
     7501 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
     7561 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
     7621 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
     7681 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
     7741 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc
     7801 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
     7861 gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga cgcacataca gcgcaccgat
     7921 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
     7981 caggtcgatt ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
     8041 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
     8101 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgcc gcttcgagtt ctttcaggtc
     8161 gataataccg ttacgctgcg gtgccaggta ggtgacttcg aaaccttcgc gctccagctg
     8221 acggcaagta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
     8281 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
     8341 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
     8401 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
     8461 ggttccgtcc atcgtcatga actgcatcat tttctcggca acacgcgggt ccaccggcgt
     8521 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
     8581 tcaatgcaag gaatcaggct accggctgga tgtacgaccg tgtttacgga gtatttagca
     8641 ctccggcctg attctgaatt ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg
     8701 tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
     8761 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
     8821 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcagccgc ctttaccctg
     8881 acaacgggtg gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
     8941 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
     9001 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
     9061 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
     9121 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
     9181 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
     9241 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
     9301 tgccattttt ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc
     9361 gacctacgat tctgtacatt gtaggcctga taagacgcgt taagcgtcgc atcaggcaac
     9421 cactgttatt cggccttgtt acctttattc tgctgctcaa tagaagccag aatcccgcgc
     9481 aggatattca attcctggct ttccgggcgc gcacgggtaa acagacggcg caatttattc
     9541 atcacctgcc ccggatggtt ttcacggata aaaccggttg ccagcagcgt ttgttccaga
     9601 tgaccataaa aacgctccag atcatcgacc agcggatacg gcgtctcttc atgctcgacc
     9661 tgctcgccat tttcctgagt tgccagccag gccatacgca cttcataggc aataacctga
     9721 accgccattg ccaggttcag cgaactgtat tccgggtttg ccgcaatcgc gacatgataa
     9781 tggcatttct gcaactcttc attggtcaag ccgacgcgct cacgaccaaa caccagcgcc
     9841 accggggtat ttgccgcttc agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc
     9901 ggccacggca gcgtgcggga gcgtgcgctg gtgcccacca ccaggctaca accggctaac
     9961 gcttcgtcga gcgtatcgac gatgtgagca ttaccaatca catcgctggc ccctgcagcc
    10021 agggcaatcg cctgggagtc gggtttcacc agtggattaa ccagccacag attggttaat
    10081 cccattgttt tcatggcacg ggcaacagaa cccatattgc cggtgtgtga cgtctccacc
    10141 agcacaattc gaatattttg cagcattgtc tttcttcatc taaagattat tcacgcatct
    10201 tatcataaaa cgaagacaga tgccgatctc gctgctatac tctgcgccgt tttcccgttc
    10261 tttaacatcc agtgagagag accgatgcat ccgatgctga acatcgccgt gcgcgcagcg
    10321 cgcaaggcgg gtaatttagt tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc
    10381 cagaaaggca gtaacgattt cgtgaccaac gtagataaag ctgccgaagc ggtgattatc
    10441 gacacgattc gtaaatctta cccacagcac accatcatca ccgaagaaag cggtgaactt
    10501 gaaggtactg atcaggatgt tcaatgggtt atcgatccac tggatggtac taccaacttt
    10561 atcaaacgtc tgccgcactt cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa
    10621 gttgctgtgg tatacgatcc tatgcgtaac gaactgttca ccgccactcg cggtcaaggc
    10681 gcacagctga acggctaccg tctgcgcggt agcaccgctc gcgatctcga cggtaccatt
    10741 ctggcgaccg gcttcccgtt caaagcaaaa cagtacgcca ctacctacat caacatcgtc
    10801 ggcaagctgt tcaacgaatg tgcagacttc cgtcgtaccg gttctgcggc gctggatctg
    10861 gcttacgtcg ctgcgggtcg tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat
    10921 ttcgcggcag gcgagctgct ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt
    10981 ggtcataact atatgctgac cggtaacatc gttgctggta acccgcgtgt tgtgaaagcc
    11041 atactggcga acatgcgtga cgagttaagc gacgctctga agcattaatg actcaggcgg
    11101 gtgatatcac tcacccgccc tcgcctttca ggcgctattc cgaaatactt cctcaccgct
    11161 ttactttctt tcgtcactct cccaccatct ttccccgatt aatggatgat aaagaagtcg
    11221 caaggaaagg ttatggcact gccagtgaac aaacgcgttc ccaaaattct gtttattctc
    11281 tttgttgttg ccttctgcgt ttatttagtg ccgcgcgttg ccatcaactt cttctattat
    11341 cctgacgata aaatttacgg tcccgacccc tggtcggcgg aatccgtcga atttacggct
    11401 aaggacggta ctcgtctgca aggctggttt attccttctt cgacgggccc tgctgacaac
    11461 gccatcgcaa ccatcattca tgctcacggc aatgccggaa atatgtccgc ccactggccg
    11521 ctggtcagtt ggttacctga gcgtaatttc aacgttttta tgtttgatta tcgcgggttt
    11581 ggtaaatcaa aaggcactcc gtcccaggcc ggattgctgg acgatacgca aagcgccatc
    11641 aatgtggtgc gccatcgcag tgatgtaaac ccacaacagc tggtgctgtt cgggcagagt
    11701 attggcgggg cgaatattct tgctgttatt ggtcagggtg atcgtgaagg catacgtgcg
    11761 gtgatcctcg actccacatt tgcctcttat gcaaccatcg ccaaccagat gatccccggc
    11821 agtggctact tacttgatga gagttacagc ggcgaaaatt acatcgccag cgtcagcccg
    11881 atcccgcttt tactcattca cggtaaagcc gatcacgtta tcccatggca gcacagcgaa
    11941 aagttgtata gcctggcaaa agagccaaaa cggttgatcc taatcccgga tggcgaacac
    12001 attgatgctt tttccgatcg tcacggcaat gtttatcgcg aacagatggt ggactttatc
    12061 cttagcgcgt tgaatccgca gaactaaccc atgatcgcta gcacgataat cattcacaaa
    12121 accaccttaa gacatgctaa tccactggtc agaacagttt aaaatgagaa aaattctgtg
    12181 acgcttgcca acatttctga tgattagcat tcccttcgcc atttccctga gcaaacttta
    12241 gctattctta tcaattatgc ttatgggaga tctacagatg atgcctacgc ttgctccacc
    12301 atctgtcctt tcggctcccc agcgccgctg ccagatcttg ctgacgctct ttcagccggg
    12361 gttgaccgcc accatggcaa ccttcagcga gcttaatggt gtggatgatg atattgccag
    12421 tcttgatatc agcgaaacag gacgggagat cctgcgctat catcaactca cactgacgac
    12481 tggttatgac ggtagctacc gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt
    12541 tcactggcta cgacgtggtt tccgtctgtg tccgtcattt attaccagcc aattcacccc
    12601 cgccctgaag agtgaactga agcggcgcgg aattgcgcgt aacttttacg acgataccaa
    12661 tctacaagcg ttagtgaatc tctgctcccg acggctgcaa aaacgctttg aatcgcgcga
    12721 tattcatttc ctgtgtctgt atctgcaata ttgtttgctg cagcaccacg ctggaattac
    12781 gcctcagttt aatccgctcc aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc
    12841 gcaggaaatt ggacgccact ggcagcgtcg ggcgctccag catgtaccgc ctgatgagcc
    12901 actgtttatg gcattacttt tttccatgtt gcgggttccc gatccactgc gggatgcgca
    12961 tcagcgggac agacaattgc gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct
    13021 gggaaatgtt cgtttttatg atgaacaggg gttatgtgat cagctttata tccacctcgc
    13081 ccaggcgtta aatcgcagtt tgtttgccat cggtattgat aataccctgc cggaagagtt
    13141 cgccagactg tatccacgcc tggtacgcac cacccgcgcg gcgctggccg gatttgaaag
    13201 tgaatacggc gtccatcttt ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc
    13261 ctggctaatg caggaaaacg atctgcatga aaaacagatt attctactga ccgggaatga
    13321 tagcgagcga gaagcgcaga ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa
    13381 cattaagcat atgtcggtaa aggcattttt gcagacaggc gctccgcgtg gcgcggcact
    13441 gattattgcg ccttatacca tgccgttacc gctcttttca ccaccgctga tctatacgga
    13501 cctgacgctg acaacacatc aacaggagca gatccgcaaa atgctcgaat cagcatgagg
    13561 gaacaacttt cgggcgcaaa aacattgccg gaagcgccac cagcgccatt acccagaaca
    13621 cgccgtggcc cagatattga tacaggaaac cggcgaaaac ggtcatgata gcgatactgc
    13681 cgcccatcgc gacggcagag taaaccgcct gtaaacggat gacttcgcta acctggcgag
    13741 cagcaatata acgcatggcg gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt
    13801 gcaccactat caaccacggc aacgccgtag ttgctcccat aatgccccag cgcactacgc
    13861 cgcaaatcgc cgagatcaac agcatatcgc gagcactaca acggcggaaa agtttattac
    13921 tcagcgcaaa gataatgact tccgccacca cgcccagcga ccacaaatac cccaccgccg
    13981 aggccgagta gccagctgcc tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg
    14041 ccccctgcaa taaacaaacg caggccagaa agcgccagtt ctggcgaacc agcgccaacc
    14101 acgcagacca accggtgctc tcctgctggc ggcttgcccc ttgtggctga atcgtcggac
    14161 ggatgagaaa gccgagcagc atggatgcca cgcccaacgt caacagcacg agaatcaccc
    14221 gataatcaaa catagtgacc agtttgcccg tcagcgccga gccaatgaca aacgccaccg
    14281 agccccacag tcgcactttg ccgtaatcaa gcgggaactg cttttgccac gtattcgcca
    14341 gtgcatcggt caacggtacc agcggtgaga aaaagaggtt aaagccaatc atcaccagca
    14401 tcagccacgc tacgtgcgcc cccgcccaga aggcgacagc aaagagaagt ggcagcagtg
    14461 ccagcacgcg caaggcggaa atcaggcggg aaggatcgct gacgcggggc gcgatgagca
    14521 aactcccgag aaaacgggca accagacctg cccccaataa caggccgatg gtttctggcg
    14581 ttaaaccaat ccctttaagc cagacgctcc agaaaggtaa aaaaatgccg taactaaaaa
    14641 agtatgtgaa atagccgagc gccaaccagc gcgtggattg caaaaccatg agtccctccc
    14701 gtcaggaggc gttagtctgg ggttaatcga atgttggtgc aagttgaaaa ataagaatat
    14761 tcgagcctgc ttcccgatgt ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg
    14821 cgacgcatgt tgcaaaaata atgcgtaatt atgccgttac gcttgccaaa cgttcctgca
    14881 caatggcgat gaaatcgcgt aatgccagct tcatctcccc tttcttccac gccatcagta
    14941 aagcaatgga aggcacattt ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat
    15001 tattcatata accgggtatc aaagtgacgc ccagccccat gcccaccaga ttcatggtca
    15061 ccagaatatt cgttgccacc tggacgatat ttggctggct gttttcttgc gcaaaccacg
    15121 ctttaacgat cggcgcaagc gaaccggaat acgccggatc ggtactgacg aaattcacgc
    15181 catccagttg cgcggcggtg atctcttttt catgtgctaa agggtgatca accggtaaca
    15241 ccaccactaa tggttcgtca aaaagctcca gataatcaat ctccgggcta taaacaggat
    15301 ggcgcattaa gccgacgtcg agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga
    15361 ttaaactcac cagctcaatc aaggtgtctg gctgtctgag acgaaacatc ggtaatactt
    15421 ttggcaataa attcacttcc gccgatggca caaagccaat ggttaattgt ctgtcttcct
    15481 gaacaatttt ccgcgcccgt aatttggcat tttccgcttg ttcgaggatt gccagcgcgt
    15541 cctggagaaa acattctccc gccgccgtca acgcgacttt gcgcttatcc ctcaccagta
    15601 acggaacacc gacacagttt tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg
    15661 tatgcagttt ttccgccgca cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc
    15721 gtaaatgccg tagttccatc accttcccct tgttatcgaa aaaacgtctc agctggtaga
    15781 aattaactat ttcacatatt agcaaccaac cagcaacatc cttatggcac aaaaatagaa
    15841 ggtcaataca tcttatcttt caggattaaa aaatatgacc acaccctcag atttgaacat
    15901 ttaccaactg attgacaccc aaaacggtcg ggtcactccg cgtatttata ccgacccgga
    15961 catttaccaa ctggagcttg agcgtatttt cggtcgttgc tggctatttc tcgcccacga
    16021 aagccagatc ccaaaacccg gtgatttctt taacacctac atgggagaag atgcagttgt
    16081 cgtggtgcgc cagaaagacg gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc
    16141 catgcgtgtg agttatgcag attgtggcaa cactcgcgcc tttacctgcc cgtatcacgg
    16201 ctggtcttat ggcattaacg gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc
    16261 acaagggttg tgtaaatccc actgggggct aaacgaagtt ccttgtgtgg agagttataa
    16321 aggactgatt tttggcaact gggataccag cgcaccgggc ctgcatgatt acctgggtga
    16381 cattgcctgg tatctggatg gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg
    16441 cggcgtacaa aagtgggtga tcaactgtaa ctggaaattc ccggcagagc agttcgccag
    16501 tgaccagtat catgctctgt tcagccatgc ttctgccgtt caggtattag gggcgaaaga
    16561 tgatggcagc gataagcgcc tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc
    16621 caaagatgcg ctgcaatttg gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa
    16681 accggatgct aatgtctggg tcgatggcgc agtttcaagc tattaccgcg aaacctatgc
    16741 cgaagcagaa caacgtttag gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat
    16801 attccccacg ctttcatggc taaacggcac tgccacactc cgcgtctggc atccgcgcgg
    16861 ccctgatcaa gttgaagtgt gggcgttctg tattactgac aaagccgcct ccgatgaagt
    16921 taaagccgct tttgaaaaca gcgccactcg tgcttttggt cctgctggtt ttctcgagca
    16981 ggatgactcg gagaactggt gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa
    17041 cagcaaactg tgtctggaaa tggggcttgg tcaggaaaag cgccgcgacg acggcattcc
    17101 tggcattact aactatatct tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc
    17161 cgatcttctg agtagcgaaa gctggcagga agtgctcgat aaaaccgccg cttaccagca
    17221 ggaggtgatg aaatgagtgc gcaagtttca ctggagttac atcaccgcat tagccagttt
    17281 ctctttcacg aagccagtct gctggatgac tggaaatttc gtgactggtt ggcacagctc
    17341 gacgaagaga ttcgttacac catgcgcacc acggttaacg cgcaaacacg cgaccgtcgc
    17401 aaaggcgtcc agccgccgac gacctggatt ttcaatgaca ccaaagacca gctggagcgg
    17461 cgaatcgccc gtctggaaac gggcattgcc tgggcagaag agccgccgtc acgcactcgt
    17521 cacttaatca gcaactgcca ggtaagcgaa accgacatcc caaacgtatt tgccgtgagg
    17581 gtgaattatc tactttatcg ggcacaaaaa gagcgcgatg aaacattcta tgttggaacg
    17641 cgtttcgaca aagttcgccg tctggaagat gacaactggc gcttgctgga acgggatatc
    17701 gtcctggatc aagcggtgat cacttcccat aacctgagtg tactgttctg atgaatcgaa
    17761 tttatgcgtg tcccgttgcg gatgtgccgg agggtgaggc tctccggata gatacctcgc
    17821 ccgtcatcgc cttgttcaac gtgggcggcg agttttatgc cattaacgat cgttgcagcc
    17881 atggtaatgc gtcgatgtca gaagggtatc tggaagatga cgccacggtg gagtgcccgc
    17941 tacacgccgc cagtttttgc ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc
    18001 cgctcaccac ttatccagta cacgttgaag gtggtgacat tttcatcgac ttaccggagg
    18061 cgcagccatg agcgatctgc ataacgagtc catttttatt accggcggcg gatcgggatt
    18121 agggctggcg ctggtcgagc gatttatcga agaaggcgcg caagttgcca cgctggaact
    18181 gtcggcggca aaagtcgcca gtctgcgtca gcgatttggc gaacatattc tggcggtgga
    18241 aggtaacgtg acctgttatg ccgattatca acgcgcggtc gatcagatcc tgactcgttc
    18301 cggcaagctg gattgtttta tcggcaatgc aggcatctgg gatcacaatg cctcactggt
    18361 taatactccc gcagagacgc tcgaaaccgg cttccacgag ctgtttaacg tcaacgtact
    18421 cggttacctg ctgggcgcaa aagcctgcgc tccggcgtta atcgccagtg aaggcagcat
    18481 gattttcaca ctgtcaaatg ccgcctggta tcctggcggc ggtggcccgc tgtacaccac
    18541 cagtaaacat gccgcaaccg gacttattcg ccaactggct tatgaactgg caccgaaagt
    18601 gcgggtgaat ggcgtcggcc cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct
    18661 cgggcaaagt gaaacctcga taatgcagtc tctgacgccg gagaaaattg ccgccatttt
    18721 accgctgcaa tttttcccgc aaccggcgga ttttacgggg ccgtatgtga tgttggcatc
    18781 gcggcgcaat aatcgcgcat taagcggtgt gatgatcaac gctgatgcgg gtttagcgat
    18841 tcgcggcatt cgccacgtag cggctgggct ggatctttaa ggaagcacga tgaaagaaaa
    18901 aacgatcatt attgtcggtg gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca
    18961 gcaagggttc accggtgagc tgcatctgtt ttccgatgag cgacatcttc cttatgaacg
    19021 acctccactc tcgaaatcca tgttgctgga agattccccg cagttacagc aggtgttacc
    19081 cgctaactgg tggcaggaaa acaatgttca tctgcattcc ggtgtaacca tcaaaacgct
    19141 gggtcgcgac acacgagagt tagtgttaac caacggcgaa agctggcact gggatcagct
    19201 ttttatagca accggcgcgg cagctcgacc gctgccgttg cttgatgcac tgggagaacg
    19261 ctgctttacc ctacaccatg ccggtgatgc cgccagactg cgagaagttc tgcagcccga
    19321 acggtcagtc gtgattgtcg gtgccggaac tattggtctg gaactggctg ccagcgccac
    19381 gcagcgcaga tgtaaggtga cagtgattga actggcggca accgtcatgg gccgtaatgc
    19441 accaccgccc gtgcaacgct atcttttaca gcgccatcag caggctggtg tgcgcattct
    19501 gctcaataat gccattgaac atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag
    19561 tggggaaacg cttcaggctg atgtggtgat ttacggtatt ggtatcagcg ccaacgagca
    19621 actggctcgc gaggccaacc ttgatactgc caatggcatt gtcattgatg aggcttgccg
    19681 cacctgcgat cccgcgatct ttgccggtgg cgatgtggca atcactcgtc ttgataatgg
    19741 tgcactacac cgctgcgaaa gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc
    19801 cgcaatgtta gggctaccgc taccgctact gccgccgccg tggttctgga gcgatcagta
    19861 cagtgataac ttacagttta ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa
    19921 cccggaaact cagaaggcga tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt
    19981 cacgctgaat cagggccgtg agattcgccc aattcgcaaa tggatccaga gcggcaaaac
    20041 gtttgatgcg aaactgctga tagatgagaa catcgcgctt aaatcactgt aatcaggata
    20101 attagcgaat atctcaatgc ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa
    20161 atcacattat cttgcaaagg gattggttat gaacacacta cgttattttg attttggagc
    20221 tgcccgcccc gttttgttat taattgcccg tatcgccgtg gtcttaattt tcattatttt
    20281 tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgttgggcgc
    20341 gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt
    20401 aatcgtgctt ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg
    20461 tacggcggtg attggtcacc attactggga tatgaccggc gatgcggttg ggccaaatat
    20521 gattaatttc tggaagaatg tcagtatcgc tggcgcgttc ttgctattgg caattaccgg
    20581 accgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct
    20641 tatccggcat tttataaatt acagccactc tacccgcaac gacatctctg aggttgttga
    20701 ttcccctggt gctagcgcaa tgagatcacc cccttccggg cgatgatgat catccggcgc
    20761 atggctcatc ggttccagac agaagaaatc aaacgcatat cctttatcaa acgcagggtc
    20821 ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt tccatgatga tggcataacc
    20881 ttcctgcggc tgctcgatgc gggcctgacc attccatccg gcaaaaccat tgttcaccca
    20941 ctgacgcggc aacggcgcga gctggttaaa atccagttcc tgcggtagct gctcgcaaaa
    21001 ctcacccgcc agccactgct cccgttccag ccagtaaccg ctcgcctgcg cctgaatccg
    21061 cgtttgtggt gacaacggaa aataaggatg ccagccggta ccaaatggca gcgtctctgc
    21121 cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg
    21181 acttacccga tagtgataga caccgctgcg atgttcatac accagacaca aactatcatc
    21241 gctgtgcgag acacattgcc actcgcccag ccagccatcg ccgtgtagat agtgcgcatc
    21301 ccactcaaca ttcggttgca gttgatactc acgcccctgc cagacaaaac ggttgccgct
    21361 cacccggttt gcaaatggca ccagcggaaa acatgaggca tcggttgcca caccgctttt
    21421 tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag ccttcgatta cgccgccctg
    21481 gtcagaaacg tccagcttta gcgacccgtg ggataaggta tagatggtca tccgctaact
    21541 ccttaatccg ggaagttaat cacaactttc ccgcatttgc cgctcgccat cagcacatag
    21601 gcatcacctg cctgttctag cgagaagcga tgggtaatgg cgttacgcgg ccacagcttc
    21661 cagtcagtca aatcgtgggc gcatttttcc atatggaaca gactggtcac ccaggagccg
    21721 ataatccgcc gttgatggtg catcagatca gcgctgacct cgaattccac ttttccggtt
    21781 tcaccaatgt aaaccacccg cccccagtca gcagtggatt gcagtgccag caagcgacct
    21841 gcggcattac cggaacaatc gagcgcaaca tccgcgccac cgtgggtaag ttcagcaatt
    21901 atctgcggca gaccttcggt ggttgctaaa tagccgtgat ccatcccccc taactgtttt
    21961 gccatcgcca gacgttccgg cagcatatca acgccgatga tccgttttgc accgcgacct
    22021 ttcgccagca tcatcgccat catgccgacc ggccccagac cgaccaccag cacgttatcg
    22081 ctgccggaaa cttcgccgcg caaaatccct tcataggccg tgccaacgcc gcaactgata
    22141 aacgcaccat cttcgtagct cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc
    22201 agcagatatt cggcatgacc gccgtcacgc tgccagccgt aagccgcttt tcctttgcca
    22261 gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga
    22321 tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc cttgccccat cgccacaatc
    22381 tgcccgcacg gttcatgacc gttgataaat ccctggtata acggtttatc gggtgccgcc
    22441 gccgtggcac ggtgttgatg atagatatag tggacatcgc ttccgcaaat cccggaggat
    22501 ttcattttga tcagtacctg gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg
    22561 acggttgaat ttcctagtaa ataagcagcc agcatcgttt tcataaatcc ctcattaaca
    22621 atacgattaa ttttcatccc tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg
    22681 ctttactgct tcgctgggtc agcaagatat tcgccagcac cgccaccacg atgatgacgc
    22741 cgcgtaccac ctgctggaaa aaggagttaa taccgagcag caccagaccg ttaccgatta
    22801 gcgtaatcac cagcacacca agcaatgtac cgaacaggga accgcgaccg ccggaaagtg
    22861 ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc cagaccgttt gcggcacctg
    22921 cgttaccgga accgaggcgt gccgccaaca aaatgccggt caccgccgct aataatcccg
    22981 aaagggtaaa gataagaatg cgcactcgac gaacgttaat accgcacaac tgcgccgccg
    23041 tggcattacc gccaacggca aaaaccgagc gcccgaaggc ggtttttcgg ctaatgaaca
    23101 caaacagcgc aaacaacacc atcatgatca gcgcggatac tggcacaccg agaaattgtc
    23161 cgcccagcca gtccagcacc tcgttttcgt caatcggcac tggcagtgcg ttggtcataa
    23221 acagccccat tccgcgcaga gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt
    23281 taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag cgcgcctaac agcaacacca
    23341 gcagacaagc aatcgccaac ggaacttcaa attgcagcaa aaatgccagg cacaccgaga
    23401 caaaagccac catcggccca acgctgacat caatttcacc ggagataata atcagcgtca
    23461 tcgcccaggc ggcaatccca atggtggcgg catcgcgcag cacgttcatc tggttattca
    23521 atgagataaa gccaggcgcg ttcagggaga agaccagata aagaatggca atcaccacca
    23581 gcaaaccgat ctcattaata tggcgactga caaattgttt gagcgagacg ctcttgccct
    23641 gcggcaatgg taatgacgaa gcatacatct tcaggttcct cggtaaaatt catcagtgta
    23701 cagacagaat ggcggacatc agctcatcca cattgaccgg cgagtgaaac tcctgcgaga
    23761 acgtgccgtg ctgtaataac aggatgcggt cacacaccag cggcaattcc tccacttcac
    23821 tggagataaa caccacgctt tttccttcgg cagccagctc acggacaata cggtaaatct
    23881 gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg
    23941 cggcatagac ccaacgaccg atcaccactt tttgctgatt gccaccagaa agcgtgccga
    24001 tgggtgtttc gctactggca gccttgaccg tcatccgctg catcacctct tcggtcaggc
    24061 ggcggatggt ggaccattgc agcacaccgt tggtgctgat tttttgccga ttggtcagca
    24121 ctgtattttc gtcaacgcct agccagggaa tgatccccgc ttctttgcgg ttttctggcg
    24181 tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg gcgcatgatt ttctcgccgt
    24241 tgataacaat ttcgccctgt tcatacgtct ccagcccaac aatcgccttc agcaattcac
    24301 tgcgccctgc ccccagcagg ccagcaatgc caagcacttc gccacgacgt agcgtaaagc
    24361 tgatatcctc cagcttgggc ttatggcgta acgcacggac ttccagtacg gcctgatcca
    24421 caatttcctg aggggcaacc ggcgcaatat caacgtgatc acgccctagc attagcgaca
    24481 caatatgatg cgtggaggtg ttttcgagca tcacatcgcc cgccacctga ccatcgcgca
    24541 taacggtggc ataggaggca atgcggcgga tttcttccat ccggtggctg acataaatca
    24601 ccgccacgcc cagtgccgac atctttttca ccgcactgat caccagttca acttctgcac
    24661 tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc
    24721 gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac
    24781 taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat
    24841 caatcatgcc gttgcggcga ggccattgac caaggcagag gttttctgcc accgtcagcc
    24901 cttccaccag acttaattcc tgataaaccg cacgaacccc cagttcagcg gcacggcgag
    24961 tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat ccagatatca ccgctatccg
    25021 ggcgttcgct gccggtaagc attcgaatga gagtcgattt gcccgcgccg tttttaccta
    25081 acagcgcacg aacttcgcct ttattgagcg tgaagttaac gttatccagc gcaacgacgc
    25141 cggggtaacg cttatttcct gccaccactt ttgctaccgg gactgcctct gttgccgtga
    25201 acatagggac ctctgcgaat cagcgattag ggcagaccat caacgtgcgt tgccagccac
    25261 tgtttaccat cttccgtttt ggtatagaga tcgattggca cctgaatcac tttttcaccg
    25321 tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta
    25381 ccggaaatat ccactaccgc tttcagcacc tgattgtttt ccagctcctg agcaatttcg
    25441 gttgtcatat ccgaaccgaa aacagcaatt tttccggcct gattttgatt acgtaccgct
    25501 tttaccgcgc cgagtgtcgc accgcccgac tcccccataa tggcgttgag atccggcgtg
    25561 gagataatca gtttttcacc aacggaaatc gctttatcta aaacagtccc ttcctggtta
    25621 gcgacaattt gcgcgccggg aacgcgggat tttaatactt cttcaaatcc tttacgtcgc
    25681 tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa ttttcggctg gtcaatttta
    25741 ttggcaataa aataatcggc ggcagcgtta cccagttttt taccaaattc cagtggatcg
    25801 ccgaccagat acgccgagac atatttatcg acaccctttt gattaataca ggtgttgtag
    25861 caaatcaccg gaatacccgc ttcactggcg cgacgaacgg tacggctact gccattttca
    25921 gacactgccg ataaaataat ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta
    25981 ctttctttcg aaatatcccc ctgggcgtta gtttcaatta actgtacctg tactgaagaa
    26041 tcttttgccg catcctgaac gccctgacgc actccagcgt aatatccctg ggtatcaagg
    26101 tatattgcac caatggtcat ttctttatcc gcagccctgg caaatagtgc gctacctaat
    26161 agcgtagcca ttaataatag attacgggtt gttctcattt ttttaggcat agtgcctcct
    26221 gtagggtttt tattaacaac ggcttattct aattattttg tgatgagcgg cagcgcgtgc
    26281 cgctcaaata ttacagttaa tagggtttag tgaacgtagt taaaaataaa aggcatcaca
    26341 gtggtgaata atgccgcttt cggccaggcc ggattaatcg ccgtcaattc agcgcgagca
    26401 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
    26461 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
    26521 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
    26581 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
    26641 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
    26701 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
    26761 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
    26821 ttctccggat aatacagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
    26881 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
    26941 tccccgcctt cccacgggta gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
    27001 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
    27061 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
    27121 tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
    27181 tcaagataac gcgcggccag ctcataatcg tgtttcttat tccacgcatg gatcgctaac
    27241 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca taaggcaatg
    27301 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
    27361 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
    27421 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
    27481 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
    27541 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg
    27601 cgtccctgac acagaccgcg ccactgcgcg agggctgatt cgctacgacc atcgaaccag
    27661 ttcagccacc acagagtggc gttcagcgga taatcacgca gcagtttctc gcgctgcaaa
    27721 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaagca aagcacttcc
    27781 tgattggttg ggcaggcacg aagactttgt tggcaaaaat ccagacccgc gtcgaagtta
    27841 tcattacgcg ccgccagtcg tgccagacca taatagccgc ctgctttact attgccgctc
    27901 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
    27961 ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt tgttcagcgc atgtgcgcgt
    28021 ttcagcgcct gactggcata cgccaccgct tgcgggaaat ctgcgcggtt atattccagc
    28081 atcgccagcg ccaggttaca gcgataatcc agcgggtcca gcgccacgcc gcgcaggtag
    28141 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
    28201 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccggcaac
    28261 ggcaactcct gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
    28321 agttcaatgg tcagtctgtc aggattgata ccgtgcaaca cgccctggat ggcggtcgct
    28381 ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt tgccgatttc gcggatcgcc
    28441 agacggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgctcacta
    28501 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccag agaatgataa
    28561 ggcaggaaat actgctcgaa acgcttctct tcgtaagcat caagccaggt aaaatcaggc
    28621 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
    28681 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
    28741 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
    28801 acaaaatcgt actgcgattt ttcggccata tatgaggttg gaacaggcac atttttatag
    28861 cgagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
    28921 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
    28981 ctctgatgcc cttcaccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
    29041 gtggcgttgc cgttatagac gcggctggcg atttccagtg ccgcccggtc agggcgcagg
    29101 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgccgac ccacaccgtc
    29161 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
    29221 tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc acggtcccag catccccacc
    29281 agcgcaggtt taatgacttc attgtgatag acaaaatcgc gctgtttcac tttatcccat
    29341 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
    29401 agccacaccg cctgctagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
    29461 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
    29521 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
    29581 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
    29641 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
    29701 tgaagatgag agcctgcatt aacaatcaac agattctcca ccataacaaa tgcgtgattc
    29761 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
    29821 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
    29881 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
    29941 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
    30001 atgcttgttt aagtccgaaa ggcgaatttg agtatttaca gattgatgta ccgacgccgc
    30061 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
    30121 gcaccatcaa tctggcgctg gcaatccacg ggcaggttga tccggtgacc ggcgtatcgc
    30181 aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt gaagtatctg ctggaagaga
    30241 agctgggcat tcgggtgctg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
    30301 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
    30361 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacagattg
    30421 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggcagcctgg
    30481 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
    30541 cggttaatac tcagcttgag cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
    30601 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
    30661 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
    30721 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
    30781 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
    30841 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
    30901 ggcgcgataa cgtagaaagg cttcccgaag gaagccttga tgatcataaa cgaaaaattg
    30961 cctgatgcgc tacgcttatc aggcctacac ggagattgca atatattgaa tttgcaagat
    31021 tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg aacaacgagc acattgtcag
    31081 caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    31141 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    31201 cagccagttc tttcgcttcg gcttctttaa agccgcgacg ggtaatcgcc ggagtaccta
    31261 cacgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    31321 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcaa
    31381 ccagatcaac caggaacagg tggttatcag tgccgccgga aaccactttg tagccgcgct
    31441 cgaggaacac ttctaccatc gctttagcgt ttttcgcgac ctgctgctgg taagttttga
    31501 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    31561 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    31621 cacctttcgc caagatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    31681 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    31741 cgtgcgccat atcaacgaac agataagcac cgatgctgtc agcgatttca cgcattttcg
    31801 cccagtcaac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    31861 ctttggcttg tttttccaga tcggcgtaat cgatatgacc ggtagcatcg ataccgtaag
    31921 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    31981 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    32041 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    32101 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    32161 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    32221 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    32281 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    32341 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    32401 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    32461 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    32521 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    32581 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    32641 agatacatca attaagatgc aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct
    32701 acagtaaaag ccaccatccc tttactggtg gaaacggggc caaagttaac cgcccatttc
    32761 tacgaccgta tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag
    32821 cgtaatggcg atcaacgtga agccctgttt aacgctattg ccgcctacgc cagtaatatt
    32881 gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagcttc
    32941 cagatcaaac cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa
    33001 atgttcagcc cggggcagga agtgctggac gcgtggggta aagcctatgg tgtactggct
    33061 aatgtattta tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt
    33121 tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc
    33181 agcttcgaac tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat
    33241 ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg
    33301 actcgcaaac cggatggcaa aggctatcgt atcgcggtga aacgcgaaga gggtgggcag
    33361 gtatccaact ggttgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg
    33421 gcaggtgatt tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctaccggt
    33481 gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacacagca
    33541 caagtgaact ggttccatgc ggcagaaaat ggcgaagttc acgcctttgc tgatgaagtt
    33601 aaggaactgg ggcagtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgagc
    33661 gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg
    33721 gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg
    33781 cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc aggaaaacat tcattacgaa
    33841 tgctttggcc cgcataaggt gctgtaattt gatgttgccg gatggaaaca tccggcaacc
    33901 cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccga
    33961 gccacgtcaa agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata
    34021 atggtatcga cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc
    34081 ggcagaaaat ccaccatata ctccgcgccg cggtacagct cggtatggcc tttctggcga
    34141 ccaaagcctt tcacttcggt caccgtcatg ccggtaatac cgacttcggc cagtgcttcg
    34201 cggacatcgt ccagcttgaa gggttttata atcgcatcaa tctttttcat gctattcctt
    34261 gaaaaggttg cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg
    34321 aaatcgtttg catccagctc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc
    34381 cccgccattc tagccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa
    34441 tagttgagtt caaaatgatt acgtgcctca acaaaggttg gcagcgccgt attttcaccc
    34501 tccagcgcct gctccaccag cgcatcacta atcaccggag atgaggtcag cgccacgcac
    34561 tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc agctcgcggt catcaggcgt
    34621 ttcatcgcat cggtagagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc
    34681 aacaggtgat ttgccagtag cggaatgtct tctgtgcgct ccgccagtgc cggaattttc
    34741 aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga attccccgcg cgccatcgct
    34801 tttgacagat cacggtgagt ggcagaaata atccgcacat tgatatcaat atcgcggtta
    34861 ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgtaacggc
    34921 gcaggcatat cgccaatttc atcgagaaat agcgttccgc cttccgccgc ctggaataaa
    34981 ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg catgaccaaa cagctccgac
    35041 tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa taaatggttt gctgttgcgc
    35101 gggctggcgt tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta
    35161 atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct gttccagcaa acgcagcatc
    35221 agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc
    35281 tccagcgcat cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgaggaaa
    35341 ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga
    35401 ataattactg gcattcccgg ctgcactttc tggatttcag caaatagctg cataccgtcc
    35461 atttcatcca tccgcaggtc gctgataact aaatctactt tttcgcgatt cagtacccgt
    35521 aatccttcag cgccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg
    35581 ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata ataaatgcgc aggtttatgg
    35641 ctcatcagga gtgacctcat gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc
    35701 cggcgagaaa tttccagccg gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt
    35761 ttccagcttg cgggtggtga gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa
    35821 tgtatccagc tcgctgtcgc tggactgctg gagtttactg taacgttggc gctcttccgc
    35881 cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc
    35941 cgggatctcg gtacttaacg cttcaatacg cgcgaccagc tggcggcgct catacggcgt
    36001 aattttggcg tcggcgagca aaatcccttg tttaaaggta ttttgccagc tgccgtcgtc
    36061 atattggcga gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc
    36121 ccgcagccag taaagcggat tggtttcggt tgatttacct tgcagcgccc agatgtcgct
    36181 acattcagta gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt
    36241 atcaatggct ggcttattgt gattctgcac gcaacccagc aatgccagac atggcagccc
    36301 tgccagccac agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact
    36361 taaagcctat cccagtgggc gtaattgttg cagacagtct ggacatggac agcgcggaga
    36421 aaccggggcg tacatatcgt acgtgaagat ttcgagcact acccggggcc gaactgacaa
    36481 ataaaatagc ctgataggat aggctgttag cattatttcg tgtttttcga cgacggtaat
    36541 tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata
    36601 cggcgaatac aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt
    36661 cgctggtggc ttccctgaaa aaaggggtcg aagatcatgg cgcgttcctc ttgcggaatg
    36721 ggcgtgcctg tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg
    36781 caaatgttac cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg
    36841 ctcatcagca gcattggctc cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc
    36901 atcattttag cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct
    36961 aattcaacat tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt
    37021 tgttcgatca gtttttgcaa attgcagctg ctgctatcaa gaatgctcac cacctctttt
    37081 tgctctggcg taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata
    37141 ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat
    37201 tccagccatg acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg
    37261 ctcggtccac agaacgaaac gctattgccc agagaacgcc cttcccccag acggttgatc
    37321 atgcgctcga tatttttcac cggcccgata atcatccgcg tgaaaagtag caccatcacc
    37381 agactcacca gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt
    37441 tcacgctgaa gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg
    37501 gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg cagcatcggg accactgttg
    37561 ttacactgaa gttgggccag attgttcaaa tcctgacgca atgcctggta aagtttatcg
    37621 tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga
    37681 taaaccttcg caagcgtagg gtcgtccagc acgcaatact gacggtagct acgctccatc
    37741 tccagcgccg cgttggtcat cgcttcacta cgccgggcat caataagcgt agtgcggtta
    37801 accagcgccg cctgatcgct aagcgcattc aggctttgcc aggcttgcca tgccagcacc
    37861 aacaggggta gtagaattag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga
    37921 aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg cgaatagcct gatgctaacc
    37981 gaggggaagt tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg
    38041 gtgcctaact cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga
    38101 cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac
    38161 ataagtcggt gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat
    38221 agtttggtaa cgtactgatt atacgttgct ttgagggatt tatggcttct tcgctatttg
    38281 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagatta
    38341 caataaaatc aatcatttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    38401 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    38461 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    38521 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    38581 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    38641 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    38701 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    38761 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    38821 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    38881 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    38941 gccacaactc actccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    39001 atacccccag ggacagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    39061 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    39121 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    39181 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    39241 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    39301 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    39361 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    39421 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    39481 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    39541 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    39601 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    39661 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    39721 ccagcgttac cagcaggcct ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    39781 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    39841 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    39901 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    39961 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    40021 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    40081 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    40141 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    40201 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    40261 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    40321 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    40381 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    40441 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    40501 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    40561 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    40621 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    40681 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    40741 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    40801 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    40861 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    40921 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    40981 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    41041 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    41101 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    41161 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    41221 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    41281 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc agtaacaatg cgctcaggct
    41341 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    41401 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    41461 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    41521 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    41581 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    41641 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    41701 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    41761 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    41821 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaagcca agacgcaggt
    41881 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    41941 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    42001 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    42061 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    42121 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    42181 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    42241 gttgtgcgtg ctcatcatcg tttaacgacg cattgaggtc agcaaaatgg acatactcgg
    42301 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    42361 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    42421 agctttcggt gtacccaagg ggggggaaac gggcgtcatt ataaagaatc tgatgcgctg
    42481 acgaaaccgt ttgcgtggaa ataaaatcac catcgtgaat tagcaacgcg tgccgccaat
    42541 ggctgtaata agttgccatc tggcgcaggt ttacgcaaaa tgccgctcat ttatgagtaa
    42601 acctttcact attattacgt tttttcaagc tgggacgcgc acgacacaga gaattaacta
    42661 attgaaaaaa ttaaagatta attatctgtt catcggcatt ctggcactgc tgctcgcggt
    42721 cgctctctgg ccatccattc cctggtttgg taaagccgac aaccgtatcg ccgccattca
    42781 agcgcgggga gagttgcgtg tgagcaccat tcatactccc ctgacttata acgaaatcaa
    42841 cgggaaacct tttggcctag attacgaact ggcgaaacag tttgccgatt acctcggcgt
    42901 aaaactgaaa gtgaccgtgc ggcagaatat cagccagctg tttgacgacc ttgataatgg
    42961 taacgccgac ctgctggcgg caggactggt ctataacagt gagcgggtaa aaaattatca
    43021 gcctggccct acctattatt ccgtgtcaca acaactggtt tataaagtgg gtcagtatcg
    43081 cccacgtacg ctgggcaacc tgacggcgga gcaactcacc gttgcaccgg gtcatgtggt
    43141 ggttaacgat ctccagaccc tgaaagaaac aaaattcccg gaattaagct ggaaggtaga
    43201 cgacaaaaaa ggctctgcgg aattaatgga agatgtcatc gaaggaaaac tcgattacac
    43261 cattgctgat tctgtcgcca tcagcctgtt tcagcgcgtt cacccggagc tcgccgtagc
    43321 gctcgatatc accgatgaac aaccggtgac ttggtttagc ccgttagatg gcgataatac
    43381 cctttccgcc gccctgctcg acttcttcaa cgaaatgaat gaagacggta cgctggcacg
    43441 cattgaagag aaatacctgg ggcatggcga tgattttgat tacgtcgata cgcgcacatt
    43501 tttacgcgcc gtcgatgcgg tactgccgca gttaaagccc ctgtttgaga aatacgccga
    43561 agaaattgac tggcgtttgc tggccgctat tgcttatcag gaatcgcact gggatgcaca
    43621 ggccacttca ccgacgggtg tgcgcggcat gatgatgtta accaaaaata ccgcgcaaag
    43681 cctcggcatt acggatcgta ccgatgccga acagagcatc agcggtggcg tgcgttattt
    43741 gcaggatatg atgagtaaag tgccggaaag tgtgccggag aacgaacgga tctggtttgc
    43801 cctcgctgcg tacaatatgg gctatgcgca tatgctggat gcccgcgccc tgacggcaaa
    43861 aaccaaaggg aatcctgaca gttgggctga cgtaaaacag cgtctgcctt tacttagcca
    43921 gaaaccctat tacagcaagc tgacttacgg ctacgctcgt gggcatgaag cctacgctta
    43981 tgtcgaaaat attcgtaagt atcagattag cctggtgggt tatctgcaag agaaagagaa
    44041 gcaggctaca gaagcggcga tgcaactggc gcaggattat ccggcggtat cgcctacgga
    44101 gttgggcaaa gagaaatttc cttttctctc gtttctttcc cagtcgtcat caaactattt
    44161 gacccattct ccctctctgc tgttttccag gaaagggagt gaagagaaac aaaattaatc
    44221 cgtcgaggat tgcgcttttt tctgcgcttt aatttcctgg cggcgcatgc gaaagaagtc
    44281 actgagcaac gccgcgcact catccgccag tattccttcc gtaatttcca ctcggtgatt
    44341 catacccgga tgatgcagca catccattaa agatcccaca gcgccagttt tcgcgtcacg
    44401 cgcaccaaag accacgcgac caatgcgact gtggatcatc gctccggcac acattacaca
    44461 tggttcaagc gtgacataca acgtggcgtc gatcagacga taattttgca tcaccagacc
    44521 accctgccgc agggccatga tttctgcatg tgcggtggga tcatggcgac caatcgggcg
    44581 gttccagcct tcgccgatta cccgattgtt atgcactaat accgcgccga ccggcacttc
    44641 ccgctcatcc caggcacgtt tcgccagcgt cagcgcgtga cgcatccaat attcgtggct
    44701 aaattcgact tcagacaaaa agaaaactcc ggttataaaa gcgcggcgca ttatacacgg
    44761 acgctatgct ttactctatt ccagttgctg gagttcaccg cgcggggtta ctcgccaacg
    44821 atgctgacag aaataaagca gcggattgtc ctgattactg tcgctatagc cactgtacag
    44881 ccgcagcggc gtgccgattt tgcgctccag ttgtgcgacc ttttcatgtc ccagacaacg
    44941 catcgtcaat acccaaccac catagccacg ctgaatttgg ctggcgataa gattaacccg
    45001 cggcagccag ggcgtatcga aataaaccgc ttcaaccagc ggctgcggag agccggtaat
    45061 caaccagata tcagcatcgg aacttaacag gtaggtggtt aatcgctcct gaaccagcgg
    45121 aaaggcggta acattgtcgc gaaaccagcg cacgaaatcg gcctgcaacg tctgtaaacg
    45181 tgcttcgctg tgaccaaaag tgcaccccca cagaagcaga ctcatcggcc agcgtgccgc
    45241 acgacctttt atcaataacg caatggctat aatcggtaac aacggcagga caagtaacgc
    45301 attcagcggt tggcgacgca gtaaatagcg cagaaaactg ccgaacatat cctgctgatg
    45361 caatgttcca tctaagtcaa aaaacaccac acgacgctcg tgagttgcca aaccatactc
    45421 ctctggactg aaacatcctg attaattttc gctactacat agcctaacag atagatcatc
    45481 acttttccgg caacaatcga ccgcatcatg gctaactgga atttttaatt catggcaatt
    45541 agcggcaatg gaatataaaa ttcactcgcg tgtgtctcat attgcctgtt gtcgccacta
    45601 tttcatcgcg aggaaagata tgaacggctt acttcgtatc cgtcagcgtt accaagggct
    45661 tgcccagagt gataaaaaac tggcggatta tctgctgcta caacctgata cggcgcgcca
    45721 tttaagctct cagcaactgg ccaacgaagc cggagtcagt cagtccagcg tcgtgaagtt
    45781 cgcgcaaaaa ctcggctata aaggttttcc ggcgcttaag ttggcgttga gtgaagcgct
    45841 ggcaagccag ccggaatcgc cctccgtgcc cattcataac caaatccgcg gtgatgatcc
    45901 gttacggctg gtcggcgaaa aactgattaa agaaaatact gccgctatgt acgcaacgct
    45961 aaacgttaat agtgaagaga aactgcatga atgcgtaaca atgttgcgct ctgcgcggcg
    46021 gataattctg accggtattg gcgcttcggg tctggtggcg caaaactttg cctggaagct
    46081 gatgaagatt ggcttcaatg ctgccgcact gcgcgatatg catgcgctac tcgcaacagt
    46141 acaggcgtcg tcccctgacg atctgttatt agccatttcc tacaccggtg tacgacgcga
    46201 gttaaacctg gcggcagatg agatgctgcg agtgggcgga aaagtgctgg cgattaccgg
    46261 ctttactccg aatgccctgc aacagcgtgc ttctcattgc ctgtatacca ttgccgaaga
    46321 acaagcgaca aacagtgctt caatctctgc ttgtcacgct cagggaatgt taacggattt
    46381 gctgttcatt gcgctgattc agcaggatct ggaactggca ccagaacgta ttcgtcatag
    46441 tgaagcgctg gtgaaaaaac tggtctgagt aaagaatgcg cgtataatgc ccgcccggtt
    46501 tgtgttgttt tgagagtttc cttatggcgc tgttaatcac taaaaaatgc atcaattgtg
    46561 atatgtgtga aatcaaataa aaacacattt attttcatag tgatacagca atgtgttatt
    46621 gaatgtgttg ttggatttat ggtgcaaaaa aattttgcat aagttcagca acacaaagaa
    46681 caccactttc tttaacgtcc tccgcgccct aaaggacggg gctttacggc gcaaaaggcc
    46741 tgtgacatac aacattaact tttcagtcaa ccgtagcacg ttcttgtata cgccgtgtct
    46801 gcggttttat ataaccctgg ctggaaacct cttatacaaa gtcgattcgc caacatcata
    46861 gatgatcgcc accttctggc gagaaactcc tgatgcaatt agtcgcccgg cctgcgccca
    46921 ttattctggt gaaagtttgg gacggcgacc gccaattcgt ccctgtgcgc gagcagcttc
    46981 cagtcctgct tttgttcgtt caacaatcag ttcacgttcc atttcagcca gggcacccat
    47041 cacatggaag aaaaagcgtc ccattggtgt gctggtatca attgaatcag tcagactacg
    47101 aaagttgatg cctcgttcgc gcaactcctc caccagcacg acaagatgcc gcatactgcg
    47161 ccccagccga tccagcttcc agacaaccag tgtgtcgcct gccgataatg tcttgagcag
    47221 ttttttcagc cccggtcttt cggactttgt accacttatc ttgtcttcaa aaatcagctc
    47281 gcatcctgca cagtt
//
LOCUS       DAECNO010000021        46630 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.021, whole genome shotgun sequence.
ACCESSION   DAECNO010000021 DAECNO010000000
VERSION     DAECNO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.021"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(16..603)
                     /gene="mog"
                     /locus_tag="KKO08_001186"
     CDS             complement(16..603)
                     /gene="mog"
                     /locus_tag="KKO08_001186"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBD6317513.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(718..1671)
                     /gene="tal"
                     /locus_tag="KKO08_001187"
     CDS             complement(718..1671)
                     /gene="tal"
                     /locus_tag="KKO08_001187"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBD6317514.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1950..3379
                     /locus_tag="KKO08_001188"
                     /pseudo
     CDS             1950..3379
                     /locus_tag="KKO08_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3449..4225
                     /gene="yaaA"
                     /locus_tag="KKO08_001189"
     CDS             3449..4225
                     /gene="yaaA"
                     /locus_tag="KKO08_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBD6317515.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4378..4674)
                     /locus_tag="KKO08_001190"
     CDS             complement(4378..4674)
                     /locus_tag="KKO08_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD6317516.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4888..6174)
                     /gene="thrC"
                     /locus_tag="KKO08_001191"
     CDS             complement(4888..6174)
                     /gene="thrC"
                     /locus_tag="KKO08_001191"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBD6317517.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6175..7107)
                     /gene="thrB"
                     /locus_tag="KKO08_001192"
     CDS             complement(6175..7107)
                     /gene="thrB"
                     /locus_tag="KKO08_001192"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBD6317518.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7109..9571)
                     /gene="thrA"
                     /locus_tag="KKO08_001193"
     CDS             complement(7109..9571)
                     /gene="thrA"
                     /locus_tag="KKO08_001193"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBD6317519.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9597..9716)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9652..9729)
                     /gene="thrL"
                     /locus_tag="KKO08_001194"
     CDS             complement(9652..9729)
                     /gene="thrL"
                     /locus_tag="KKO08_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBD6317520.1"
                     /translation="MKRISTTITTTITTTITITTGNGAG"
     gene            complement(9943..10629)
                     /locus_tag="KKO08_001195"
     CDS             complement(9943..10629)
                     /locus_tag="KKO08_001195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD6317521.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11029..11169)
                     /locus_tag="KKO08_001196"
     CDS             complement(11029..11169)
                     /locus_tag="KKO08_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317522.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11265..11981
                     /gene="arcA"
                     /locus_tag="KKO08_001197"
     CDS             11265..11981
                     /gene="arcA"
                     /locus_tag="KKO08_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBD6317523.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12040..13392)
                     /gene="creD"
                     /locus_tag="KKO08_001198"
     CDS             complement(12040..13392)
                     /gene="creD"
                     /locus_tag="KKO08_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBD6317524.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13450..14874)
                     /gene="creC"
                     /locus_tag="KKO08_001199"
     CDS             complement(13450..14874)
                     /gene="creC"
                     /locus_tag="KKO08_001199"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBD6317525.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14874..15563)
                     /gene="creB"
                     /locus_tag="KKO08_001200"
     CDS             complement(14874..15563)
                     /gene="creB"
                     /locus_tag="KKO08_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBD6317526.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15576..16049)
                     /gene="creA"
                     /locus_tag="KKO08_001201"
     CDS             complement(15576..16049)
                     /gene="creA"
                     /locus_tag="KKO08_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBD6317527.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16260..17129
                     /gene="robA"
                     /locus_tag="KKO08_001202"
     CDS             16260..17129
                     /gene="robA"
                     /locus_tag="KKO08_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBD6317528.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17126..17773)
                     /gene="gpmB"
                     /locus_tag="KKO08_001203"
     CDS             complement(17126..17773)
                     /gene="gpmB"
                     /locus_tag="KKO08_001203"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBD6317529.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17825..18345
                     /gene="yjjX"
                     /locus_tag="KKO08_001204"
                     /pseudo
     CDS             17825..18345
                     /gene="yjjX"
                     /locus_tag="KKO08_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(18430..18756)
                     /gene="trpR"
                     /locus_tag="KKO08_001205"
     CDS             complement(18430..18756)
                     /gene="trpR"
                     /locus_tag="KKO08_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBD6317530.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18846..20783)
                     /gene="sltY"
                     /locus_tag="KKO08_001206"
     CDS             complement(18846..20783)
                     /gene="sltY"
                     /locus_tag="KKO08_001206"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBD6317531.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20994..22661
                     /gene="ettA"
                     /locus_tag="KKO08_001207"
     CDS             20994..22661
                     /gene="ettA"
                     /locus_tag="KKO08_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBD6317532.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22768..22935)
                     /locus_tag="KKO08_001208"
     CDS             complement(22768..22935)
                     /locus_tag="KKO08_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317533.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(22969..24201)
                     /gene="nadR"
                     /locus_tag="KKO08_001209"
     CDS             complement(22969..24201)
                     /gene="nadR"
                     /locus_tag="KKO08_001209"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBD6317534.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24222..25604)
                     /gene="radA"
                     /locus_tag="KKO08_001210"
     CDS             complement(24222..25604)
                     /gene="radA"
                     /locus_tag="KKO08_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBD6317535.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25653..26621)
                     /gene="serB"
                     /locus_tag="KKO08_001211"
     CDS             complement(25653..26621)
                     /gene="serB"
                     /locus_tag="KKO08_001211"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBD6317536.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26727..27371
                     /locus_tag="KKO08_001212"
     CDS             26727..27371
                     /locus_tag="KKO08_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBD6317537.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27399..28420
                     /gene="lplA"
                     /locus_tag="KKO08_001213"
                     /pseudo
     CDS             27399..28420
                     /gene="lplA"
                     /locus_tag="KKO08_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28452..28715
                     /locus_tag="KKO08_001214"
     CDS             28452..28715
                     /locus_tag="KKO08_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6317538.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28876..29595)
                     /gene="deoD"
                     /locus_tag="KKO08_001215"
     CDS             complement(28876..29595)
                     /gene="deoD"
                     /locus_tag="KKO08_001215"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBD6317539.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29652..30875)
                     /gene="deoB"
                     /locus_tag="KKO08_001216"
     CDS             complement(29652..30875)
                     /gene="deoB"
                     /locus_tag="KKO08_001216"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBD6317540.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30927..32249)
                     /gene="deoA"
                     /locus_tag="KKO08_001217"
     CDS             complement(30927..32249)
                     /gene="deoA"
                     /locus_tag="KKO08_001217"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBD6317541.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32376..33155)
                     /gene="deoC"
                     /locus_tag="KKO08_001218"
     CDS             complement(32376..33155)
                     /gene="deoC"
                     /locus_tag="KKO08_001218"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBD6317542.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33413..34954
                     /locus_tag="KKO08_001219"
     CDS             33413..34954
                     /locus_tag="KKO08_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBD6317543.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVGILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34926..35789
                     /locus_tag="KKO08_001220"
     CDS             34926..35789
                     /locus_tag="KKO08_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBD6317544.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(36003..36785)
                     /locus_tag="KKO08_001221"
     CDS             complement(36003..36785)
                     /locus_tag="KKO08_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD6317545.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36782..37855)
                     /locus_tag="KKO08_001222"
     CDS             complement(36782..37855)
                     /locus_tag="KKO08_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBD6317546.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37977..38138)
                     /locus_tag="KKO08_001223"
     CDS             complement(37977..38138)
                     /locus_tag="KKO08_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBD6317547.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38255..38860)
                     /gene="osmY"
                     /locus_tag="KKO08_001224"
     CDS             complement(38255..38860)
                     /gene="osmY"
                     /locus_tag="KKO08_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBD6317548.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39253..40842)
                     /gene="prfC"
                     /locus_tag="KKO08_001225"
     CDS             complement(39253..40842)
                     /gene="prfC"
                     /locus_tag="KKO08_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBD6317549.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40933..41610)
                     /gene="yjjG"
                     /locus_tag="KKO08_001226"
     CDS             complement(40933..41610)
                     /gene="yjjG"
                     /locus_tag="KKO08_001226"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBD6317550.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41625..42071)
                     /gene="rimI"
                     /locus_tag="KKO08_001227"
     CDS             complement(41625..42071)
                     /gene="rimI"
                     /locus_tag="KKO08_001227"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBD6317551.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42040..42453)
                     /gene="holD"
                     /locus_tag="KKO08_001228"
     CDS             complement(42040..42453)
                     /gene="holD"
                     /locus_tag="KKO08_001228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBD6317552.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42556..43587
                     /gene="rsmC"
                     /locus_tag="KKO08_001229"
     CDS             42556..43587
                     /gene="rsmC"
                     /locus_tag="KKO08_001229"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBD6317553.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            43864..43950
                     /locus_tag="KKO08_001230"
     tRNA            43864..43950
                     /locus_tag="KKO08_001230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43898..43900,aa:Leu,seq:cag)
     gene            43979..44065
                     /locus_tag="KKO08_001231"
     tRNA            43979..44065
                     /locus_tag="KKO08_001231"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44013..44015,aa:Leu,seq:cag)
     gene            44100..44186
                     /locus_tag="KKO08_001232"
     tRNA            44100..44186
                     /locus_tag="KKO08_001232"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44134..44136,aa:Leu,seq:cag)
     gene            complement(44225..44461)
                     /locus_tag="KKO08_001233"
     CDS             complement(44225..44461)
                     /locus_tag="KKO08_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBD6317554.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            44602..45390
                     /gene="fhuF"
                     /locus_tag="KKO08_001234"
     CDS             44602..45390
                     /gene="fhuF"
                     /locus_tag="KKO08_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBD6317555.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(45428..46105)
                     /gene="bglJ"
                     /locus_tag="KKO08_001235"
     CDS             complement(45428..46105)
                     /gene="bglJ"
                     /locus_tag="KKO08_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBD6317556.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(46063..>46630)
                     /locus_tag="KKO08_001236"
     CDS             complement(46063..>46630)
                     /locus_tag="KKO08_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBD6317557.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        11047 a        12699 c        11740 g        11144 t
ORIGIN      
        1 ctcgggggga tttttttatt cgctaacgtc gcgtcttgca ctcttcggtc tgaatgcagc
       61 aaccacttct ggtgccgttt caacgtatgg cccttccagc aactgaatgc agtacggtac
      121 gctggcaaaa ataccatgca ccacaacgtt accctcagcg tccttcacac cttccagcgt
      181 ctctttaata gacttcggct gaccgggtaa gttaaggatc agcgcctgtt tgcgaatcac
      241 gcccacctga cgcgaaagga tcgcagttgg tacaaaatgc aggctgatct ggcgcatctg
      301 ttcaccaaag ccaggcatct cgcggtccgc tactgccagc gtcgcatcgg gcgttacgtc
      361 acgacgcgcc gggccagttc cgcccgtggt gagcaccaga tggcaactca tttcatccac
      421 cagctcacac aacgtttgct cgatgatcgc ctgctcatcg gggattaagc gggtttccag
      481 ttcaaacggc gtggttagcg ccgatgtcag ccattcttcc agcgctggaa tgcctttatc
      541 ctgataaacg ccgctggatg cgcgatcaga gatggaaact aagccaatac gtaaagtatt
      601 catcaagatt tttccgttta aacaatctcg ttaaacagaa tgatacactg cgaagggagt
      661 gacagacagg cttcagaaga ggtagcgtga ccgacttccc ggtcacgcta agaatgatta
      721 cagcagatcg ccgatcattt tttccagttt ttcctggtca atagcaaact tacggatacc
      781 ttccgccagt ttatctactg ccattggatc ctggttgtgc tgccacagga actcggactc
      841 agtgatacgc gccggacgcg ctttcacttc gccggtgtaa gacagtttac gttcgatagc
      901 cccttcgctc tccgccagct ctttcagcag tgccggtgcg atggtcagac ggtcacagcc
      961 tgccagttcc agaatttcgc cgatgttacg gaagcttgcg cccataacca cggtttcata
     1021 accgtgctct ttgtagtact ggtagatttc agatacagaa accacgcccg gatcttctgc
     1081 cggagcgtac tctttcttat cggtattcgc tttgtaccag tcaagaatac ggccaacaaa
     1141 cggcgagatc aggaacacgc ccgcctccgc acaagcacga gcctgagcga aggagaacag
     1201 cagggtcagg ttacagttga taccttcttt ttccagctgt tctgcagcac ggataccctg
     1261 ccaggtagaa gccagtttga tcagaatacg atcgttgcta atacctgcat cgttgtagag
     1321 tttgatcagg cgttttgctt tcgcaattga cgcttcggtg tcataggaaa gacgcgcatc
     1381 aacttcagtt gagatacggc ccggaaccag tttcaggatt tccagaccaa tatttactgc
     1441 cagtttgtcg gtcgcgtcca cgatctgctg cgcgcgatcg ttgctctgct gtttcgccca
     1501 ggcgacagca tcatcaatca acttacggta ttccggaatc tgcgctgcgt taagaatgag
     1561 agaagggttg gttgtggcat cctgcggttg atacagcttc attgccgcga tgtccccagt
     1621 gtcggccact acggtggtgt actgacgaag ggaggtcaat ttgtccgtca tgatagtatt
     1681 tctctttaaa cagcttgtta gggggatgta accggtctgc cctgatgata tcacgacgca
     1741 acgtcagcgc aaccgccgac aagacggtta tcgccaggcg agactgtttc ggatttctga
     1801 cactgcgttg atatcgccca ccatttttat acaaaacctc atgtatgcta cgcagaagtt
     1861 atcaagtacc tcgtagcgta tatacttctt aaacaattgg taacgtttac acaggaaagt
     1921 catcgcgacc ggcaataaga gggatatgca tgccagattt tttctccttt attaacagcg
     1981 tcctttgggg atcggtaatg atttacctgc tcttcggcgc aggttgttgg ttcacttttc
     2041 gcaccggatt tgtgcagttt cgctacatcc gccagtttgg caaaagtctt aaaaatagca
     2101 ttcatccaca gccaggcggt ttaacctcat ttcagtcatt gtgtaccagt cttgcggcgc
     2161 gcgtgggtag cggcaacctg gccggcgttg cgctggcaat taccgccggt ggaccaggtg
     2221 ccgtcttttg gatgtgggtt gctgcgttta tcggcatggc gacctcgttt gccgaatgtt
     2281 cccttgcaca actttataaa gaacgtgacg tcaatgggca gtttcgtggc ggaccggcat
     2341 ggtatatggc gcgcgggctg gggatgcgct ggatgggcgt tctgttcgcc gtctttttgc
     2401 tcatcgccta cggcataatt ttcagcggag ttcaggcgaa cgccgttgcc cgagccctga
     2461 gtttttcttt tgattttcct ccgctggtga cgggcattat tctcgctgtc tttgctctgc
     2521 tggcaatcac tcgcggtctt catggcgtcg cccggctcat gcaggggttt gtcccgttga
     2581 tggcgataat ctgggtactg accagcctgg tgatttgcgt aataaatatc gggcaacttc
     2641 cccacgtcat ttggtctatt tttgagagtg cttttggctg gcaggaagcg gcaggcggcg
     2701 cggcgggata taccttaagc caggcgatta ctaacggttt tcagcgcagt atgttttcca
     2761 atgaggtggg aatgggatcg acgccaaacg cggcagcggc agctgcatcc tggcctccgc
     2821 atccggcagc acaagggatt gtccagatga ttggcatttt tatcgacacc ctggtcatct
     2881 gtacggcaag cgccatgctg atattactgg cgggtaacgg cacaacctac atgccgctgg
     2941 aaggtattca gcttatccag aaggcgatgc gggtgctaat gggttcctag ggtgctgaat
     3001 ttgttaccct cgtggttatt ctgtttgcct tcagctccat cgttgccaac tacatttatg
     3061 ccgaaaacaa tctcttcttt ttacgcctga acaaccctaa agcgatctgg tgtttgcgga
     3121 tctgcacctt cgcaacgatc atcggcggca ccttgctaag ccttccgctg atgtggcaac
     3181 tggcagatat ataatggcct gcatggctat taccaatttg accgccattt tactgctctc
     3241 gcctgtggtt cataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc
     3301 ggtgtttgat ccgttgcgtt atccggatat cggtcgccaa ctttctccgg acgcgtggga
     3361 tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt
     3421 cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct
     3481 tgattaccaa agcccgttga ccaccacgcg ctatacgctg ccggagctgt tagacaattc
     3541 ccagcagttg atccatgaag cgcggaaact gacgcctccg cagattagca cgctgatgcg
     3601 catcagcgac aaactggcgg gtatcaacgc cgctcgcttt catgactggc agccagattt
     3661 cacgccggcg aatgcccgcc aggcgattct ggcgtttaaa ggtgatgtct acaccggctt
     3721 gcaggccgag accttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct
     3781 ttccggcttg tatggcgtac tgcgcccgct cgatttaatg cagccttatc gtctggaaat
     3841 ggggatccgt cttgagaatg cccgagggaa agatctgtat caattctggg gagatatcat
     3901 caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga ttaatctggc
     3961 gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc
     4021 ggtgttcctc gatgagaaga acggcaaatt taagatcatc agcttctacg ctaagaaagc
     4081 acgcggtctg atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac
     4141 tggttttaat agcgaaggtt acttctttga tgaagattcc tccagcaatg gcgaactggt
     4201 gtttaaacgc tacgagcagc gttaagtttg tagtcaatgc cggatgcggc gtgaacgcct
     4261 tatccggcct acaaaagcat gcagattcaa tatattgcag aattaacgta ggcctgataa
     4321 gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat ttgtcattta
     4381 gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgaggagctt tcttatggtg
     4441 gcgcggcggt gacggcggtc cgtgtgggtg ccagcgattg cctcgccatt cataatgttg
     4501 tttccaccag ccgtggtcgc gccagtgacc tccatcccag taatagccac gattatcacg
     4561 atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct gtgctgccat
     4621 gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt tcacaggtta
     4681 ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg agaacgacgg
     4741 ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgataat ttctcctttt
     4801 tccctgtcgt ttttctccat aatttcttca taaaaaagcc gggctgcata aaagcaaacc
     4861 cggcctgatt gagataatga atagatttta ctgatgattc atcatcaatt tacgcaacgc
     4921 agaaaaatcg gcgggcagat tatgtgaaag caagggtaaa tcagcacgtt ctgccagctc
     4981 ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa atttcgccgg
     5041 atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac gcagcgcacg
     5101 atacgctacg gcagcgtgcg gctccgaggt gtagcccagt tcttttaact cacgcattgt
     5161 ctgttgcgtg gtttcatcat ccacggctgc ataacccagc tctttcagtt gccagatttt
     5221 gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt
     5281 ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcgtgcagga aacgtggcac
     5341 ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga atgacttcgc
     5401 cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa ccagctggtt
     5461 gcgcgcctcc tgcggcagct gcgcaacagc ttcaaagtag tagcaaatct gcgccagcaa
     5521 acggctgatg ttaatcgagt tagccgagtt taaccccagc gccactttca gttcttcatc
     5581 atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt
     5641 ttcgatattg ccgcccaacg tacagaacag tttttcttgc agtggactga ttttgcctcg
     5701 tggatagagg ataaccactt tcacattcgg caaaccgtag aaagcatgag ccactgcagc
     5761 tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcatccg caatatgggt
     5821 cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg gcccgtggaa
     5881 caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga aggcaaacgc
     5941 cgcgcgcacg cgctcttcca ggatttcctg tgggatttca tcaccaataa acgccgagag
     6001 gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt cagtcaggct
     6061 gaattccggc aggtcgtgcg gaaaaaacag cccctgattt ttgcccaacc cctgggttac
     6121 ggcttgcgca aagctgacct gctcgttgtg atctttcaga ttgtagagtt tcatttagtt
     6181 ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac cttcctgatt
     6241 ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg gcttgtcaca
     6301 tagagcgaac aaggtcgggc cggagccgga gataccgctc actaccgcgc cgatttccgc
     6361 gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg gttcagcgat
     6421 aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa
     6481 gccagccaga tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat
     6541 agcccgggct tctgccgtcg agactttaat tcccggatac gccagcaccc acagccactc
     6601 atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca actgcatacc
     6661 accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagatac gtccttccag
     6721 ctcgcccatc aaagccagca aacgagtgtc attaagcggc ttgccgcagt gttcattcat
     6781 cgccatcagc gccgcgacca ccgaacaggc gctggagcct aagcccgaac cgatcggcat
     6841 attcttttcc agagtcatcg ccactggaat ttgcttgcca agctcctggc aaaaacgctc
     6901 ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc
     6961 gaggttgttg agactgaatg tctctgccgc ctcaaccgtg actacatctc cgagcaatgc
     7021 accatcaacg ggtgccaccg ccgccccgag cacatcaaac ccgacgctca tattggcact
     7081 ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag agggtacgta
     7141 gcagatcggc aaagacaccg gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca
     7201 ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg ccatttttca
     7261 ctttgaacag cggatcattc ccatccactt cggcaatctt cacgcggcag acaccatctt
     7321 catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc gccacgcgcg
     7381 cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca tcaccctcgg
     7441 cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc agttcacgtc
     7501 ccgtttcacg agcgagaatc aatagtttac gcgccacatc cataccagaa agatcatctc
     7561 gcgggtccgg ttcggtataa cccatttccc gcgccagcgt ggtcgcctcg gagaaactca
     7621 tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg ccggagaact
     7681 tcaccaattc atcacctgca ttgagcagat tttgcaggtt ctcaataacc ggtaatccag
     7741 ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttccgccgca taacgcaact
     7801 gatgatagta atccatcgac gaggtgttgg cctttttgtt cggcgtaaca acgtggaaac
     7861 cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg cagtcaacaa
     7921 tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg agattaaacg
     7981 gctctttggc ttgtgcaagt tcttcctgcc agttttccag attaaggcca tgcacactag
     8041 tgagcagagc cttcgagttg gcaacgccgc agacacgtaa gtcgatatgt ttattcttca
     8101 gccagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca ccgacgccaa
     8161 tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta acgcgcacgc
     8221 cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa gatccctgag
     8281 caatggcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc
     8341 cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt tccgtcaccg
     8401 ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt
     8461 cagctcgcac acagtcgctt tgtggaacgc agaaactgat actgtattcg gaagatgatt
     8521 gcgtaatcag caccacggaa atacgggcgc gtgacattgc agcaaagacg cgcgccgcca
     8581 tgccaaccat ccctttcatc cccgggccgg aaacgctgaa cattgccatg ttattcagat
     8641 tggaaatgcc cttgaccggt aattcgtctt catcacggct ggcaccaatg agcgtacctg
     8701 gtgcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg gcgatggggg
     8761 taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc gcttcctggt
     8821 aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag gtatagaccc
     8881 cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg
     8941 cggagtagtc ggaaccgtta cgtccaagca ccaccagttc gcctttttca ttaccggcgg
     9001 tgaaacctgc catcaacacc atgtgatcag ccggaatgcg gcttgccgca atacggcggg
     9061 tggactcagc aatatcgacg gtagattcga ggtaatgccc cactgccagc agtttttcga
     9121 ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc ataatggcga
     9181 tcgacatttt ctcgccacgg caaatcagcg cagcgttgat gctatccggg cactgcccca
     9241 acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctgatcg acgaaagttt
     9301 tcaattgcgc cagcgggaac cccggctggg cggcggcgag tcccgtcaaa agttcggcaa
     9361 aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg gttttttcaa
     9421 tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg gccacctgcc
     9481 cctgcctggc attgctttcc agaatatcgg caacacgcag aaaacgttct gcatttgcca
     9541 ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct ttggtcgaaa
     9601 aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tcctgtacgc gtcagcccgc
     9661 accgttacct gtggtaatgg tgatggtggt ggtaatggtg gtggtaatgg tggtgctaat
     9721 gcgtttcatg gatgttgtgt actctgtaat ttttatctgt ctgtgcgcta tgcctatatt
     9781 ggttaaagta tttagtgacc taagtcaata aaattttaat ttactcacgg caggtaacca
     9841 gttcagaagc tgctatcaga cactcttttt ttaatccaca cagagacata ttgcccgttg
     9901 cagtcagaat gaaaagctga aaaatactta ctaaggcgtt ttttatttgg tgatattttt
     9961 ttcaatatca tgcagcaaac ggtgcaacat tgccgtgtct cgttgctcta aaagccccag
    10021 gcgttgctgt aaccagtcga ccagttttat gtcatctgcc actgccagag ccgtcagcaa
    10081 tgccatgact cgttcgcgta aagcttgcag ttgatgttgg tctgccgttg tgtcactttt
    10141 cgccggttgt tgtattaatg ttgctaattg atagcaatag accatcaccg cctgccccag
    10201 attgagcgaa ggataatccg ccaccatcgg cacaccagta agcacgtcag ccaacgctaa
    10261 ctcttcgtta gtcagcccgg aatcttcgcg accaaacacc agcgcggcat ggctcatcca
    10321 tgaagatttt tcctctaaca gcggcaccag ttctactggc gtggcgtagt aatgatattt
    10381 cgcccgacta cgcgcagtgg tggcgacagt gaaatcgaca tcgtgtaacg attcagccag
    10441 tgtcgggaaa actttaatat tatcaataat atcaccagat ccatgtgcga cccagcgggt
    10501 ggctggctcc aggtgtgcct gactatcgac aatccgcagt tcgctaaaac ccatcgtttt
    10561 cattgcccgc gccgctgccc caatattttc tgctctggcg ggtgcgacca ggataatcgt
    10621 tatacgcata ttgccactct tcttgaccaa ataaccgcga acagggtgat cactgtcaac
    10681 ttattacgcg gtgcgaattt acaaattctt aacgtaagcc gcagaaaaag ccctttactt
    10741 agcttaaaaa aggctaaact attttctgac tgcactaacg gtttaattgt taaaaaatgc
    10801 tacatatcct tctgtttact taggataatt ttataaaaaa taaatctcga caattggatt
    10861 caccacgttt attagttgta tgatgcaact agttggatta ttaaaataat gtgacgaaag
    10921 ctagcattta gatacgatga tttcatcaaa ctgttaacgt gctacaattg aacttgatat
    10981 atgtcaacga agcgtagttt tattgggtgt ccggcccctc ttagcctgtt atgttgctgt
    11041 taaaatggtt aggatgacag ccgtttttga cactgtcggg tcctgaggga aagtacccac
    11101 gaccaagcta atgatgttgt tgacgttgat ggaaagtgca tcaagaacgc aattacgtac
    11161 tttagtcatg ttacgccgat catgttaatt tgcagcatgc atcaggcagg tcagggactt
    11221 ttgtacttcc tgtttcgatt tagttggcaa tttaggtagc aaacatgcag accccgcaca
    11281 ttcttatcgt tgaagacgag ttggtaacac gcaacacgtt gaaaagtatt ttcgaagcgg
    11341 aaggctatga tgttttcgaa gcgacagatg gcgcggaaat gcatcagatc ctctctgaat
    11401 atgacatcaa cctggtgatc atggatatca atctgccggg taagaacggt cttctgttag
    11461 cgcgtgaact gcgcgagcag gcgaatgttg cgttgatgtt cctgactggc cgtgacaacg
    11521 aagtcgataa aattctcggc ctcgaaatcg gtgcagatga ctacatcacc aaaccgttca
    11581 acccgcgtga actgacgatt cgtgcacgca acctgctgtc ccgtaccatg aatctgggta
    11641 ctgtcagcga agaacgtcgt agcgttgaaa gctacaagtt caatggttgg gaactggaca
    11701 tcaacagccg ttcgttgatc ggccctgatg gcgagcagta caagctgccg cgcagcgagt
    11761 tccgcgccat gcttcacttc tgtgaaaacc caggcaaaat tcagtctcgt gctgaactgc
    11821 tgaagaaaat gaccggccgt gagctgaaac cgcacgaccg tactgtagac gtgacgatcc
    11881 gccgtattcg taaacatttc gaatctacgc cggatacgcc ggaaatcatc gccaccattc
    11941 acggtgaagg ttatcgcttc tgcggtgatc tggaagatta atcggcttta ccaccgtcaa
    12001 aaaaacggcg ctttttagcg ccgtttttat ttttcaacct tatttccaga tacgtaattc
    12061 atcgtccgtt gtaacttctt tactggcttt cattttcggc agtgaaaacg cataccagtc
    12121 tatattacga gtcacaaaca tcatgccggc cagcgccacc accagcacac tggttcccaa
    12181 caacagcgcg ctatcggcag agttgagcag tccccacatc actccatcca gcaacaacag
    12241 cgcgaggata aacaacatgc tattgcgcca gcctttcaat accgcctgca aataaatacc
    12301 gttcattaac gccccaatca gactggcgat tatccatgcc acggtaaaac cgatatgttc
    12361 agaaagcgcc agcaagagca aataaaacat caccaatgaa agccccacca gcaaatattg
    12421 cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca aagaacgcca taaaagtcag
    12481 tgcaatcagc agaatggcgt acttagtcgc ccggtccgtt aattggtatt gatcggctgg
    12541 cgtcgtcact gcgacgctaa acgcggggaa gttttcccag ccggtatcat tgcctgaagc
    12601 aaaacgctca ccgagattat tagcaaacca gctgctttgc cattgcgcct gaaaacctga
    12661 ctcgctagct tcccgtttgg ctggtagaaa atcacctaaa aaactgggat gcggccagtt
    12721 gctggttaag gtcatttcgc tattacgccc ggcaggcacc acagaaagat cgccggtacc
    12781 gcttaaattc agtgccatat tcagctgcag gttctgctta cgccagtccc cttcgggtaa
    12841 agggatatgc acgccctgcc cgccttgctc taacccggta ccgggttcaa tggtcagtgc
    12901 cgttccgtta acttcaggcg ctttcaccac accaatacca cgcgcatccc cgacgctaat
    12961 cacaataaag ggcttgccta agatgatatt tggcgcgttg agctcgctaa gacgcgaaac
    13021 atcgaaatcg gcttttagcg ttaaatcact gtgccagacc tgaccggtat aaatccctat
    13081 cttgcgttct tccacgttct gattgccatc aaccatcaat gactcaggta acaaaaaatg
    13141 gataaaactt cgtttccgct ccacggtttt atcctcttcc tgcaccgtat aaagctcggt
    13201 cacaggaata gcgataagcg gcccaacgag tttttgcggc ccgctggtac tttggcgaat
    13261 cgcatcttcc acatcgctac ggtaatcggc acgttcgaca atcacttgcc gaatcagcat
    13321 tatcggaatc aacaacagca atactgcacc aaacaaggtg gtcattttcc agaacagggg
    13381 ggatttcaac atagtcttct cctttgcaat ggcagcagga tacgaaggct atgtgggaag
    13441 aatttgaagc tatgtgaagt gacggtgaag tcgtagcgag gccagcacgc caccttcctg
    13501 cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg acctcactga cgaacgccaa
    13561 ccccagaccg ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc gttcaaaaat
    13621 acgtgacagc gcgtagtcag gaataccact accggtatcc agcaccttaa gcgtgacgtg
    13681 ttcctgatcc acttcggcgc ttagcgttat gcaaccgctc tcgggagtaa aatcgatggc
    13741 gttatccagt aaattcccca gcgcctgatc cagtaacgcc ggttcagcag caacattaac
    13801 ctcggtgggc ataacatgca aagtgatgtt tttttctgcc aactgcacgg tgcgcgcttc
    13861 gctgacgcgg cgaaataatg ccgccacatc aacagcagtc agaacgactt cctgacgatt
    13921 ctccagtctt gcctggcgta gtaacgtttc caccagcgcc tgcattcgcg cattttgtgt
    13981 cagaatgttg tcagtaaaac gagccactac ttcaggcggc ggaccttcgc gtaaaatttc
    14041 cgccgcgcca cgaatcgccg ccagtgggct ttttagctca tgggttaacg cataaacata
    14101 ctgctcaata tagtttttcc cttccagctt cacgcgcata ctttccagcg cctgcgcgag
    14161 tttacgcagc tcgctactac cgagatcggg aagaggaacg ggcttattgt cagtgacgga
    14221 atcagcatag cgagtgagcc gggcaatgga gcggttgatc caccaaacca tgcctgcgcc
    14281 aatcaccagt gcaatcccca acaaaatggc gctggcccat aaaattcgcc gctcgctgcg
    14341 tttaatgacc ggagccatcg ccgcgttcgg tttgcctacg ctcaaaacgc caataagccg
    14401 cgagccgccc ataatcggcg cggcaacata catcaccgaa ctttcgggat cggcaggatt
    14461 ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt agccagacgt cattccagcg
    14521 cgaataatcc tgtccaacgg ctttatttgc cgaatcgaac aatactttgc catgcgcatc
    14581 ggtcatatag acatgatatt cattgcgcac tttgttaatg ccaccaatat tggcgcgaaa
    14641 cgggcgatgt tgtagctgat taaacgcctg cgccagttgc ccatgcgttg ggtccccaga
    14701 gagcaaatcg ggacgcgcca gctccgcaag taacgttgcc gtgtcgatca acgtcccctc
    14761 cgttgctctt cgcacgcccg gtttaacttc tttgacaaaa atagccagta cgaaccaggc
    14821 cgccaccgcc accagtaaaa aatagcccag cagcaaccgc atgccgatac gcattacaag
    14881 cccctcaggc tatatcccat gccgcgatga gtattaatcg gtgaaagatc ggggttgatg
    14941 gcgcgcagct tggcacgcag cgttttaatg tggatatcga cggtgcgatc gtaggtgtcc
    15001 tgcgcatctt cccacacgct atccattagt tgctggcggg accagacgcg gcctggcgac
    15061 ttgagtaacg tcttcagcaa taaaaactca taccgcgtca gcgttaatgg cgtgtcaaac
    15121 cagctgatct gcgccgcggg ttcattcaat tcaaaatgac caatacggat gacgggagac
    15181 ggcgtcgaga acttcttcac ccgacgcagt aaggtgcgca ccctggcgca cacttcgcgg
    15241 ggtgaaaacg gtttagccac atagtcgtca gcgccaattt ccagcccaag caggcgatcg
    15301 acctcttcac ttcgggccgt caggaacagt acaggtaacg ccggatggag cgccagtaac
    15361 tggcggcaca attcaaagcc actgatatca ggcaaaccaa cgtcgagaat catgacgtcg
    15421 ggaacctgct ggcgagcttt atccagcacc ggcaaacctc gctcaaagac ctcgacggca
    15481 aaaccttcct gctgcaacat gtagaccagc gtgtcggcta tcccttgctc atcttccact
    15541 aaccagaccg tttcccgttg cataaatcgc ctctgttatt gccgccacgg catgacagga
    15601 accgcgctaa tcgcgttttt cggcgaacct tctacaactt tgtcggagta agccagataa
    15661 gcgagcgcgt tgcgtttggc gtcataaaag cgcacgacct gtaacgactt aaagaccagg
    15721 gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc cgtttttaat acgatccgac
    15781 agttcaatcg gcccgacttg ctgacaagaa atggccgcat cggaggtatc ttctgccagg
    15841 cccaatcccc ctttaatacc accggttttc gcccggctca cataacaggt gacatttttc
    15901 acatcgggat cgtcaaaggc ttccacaaca attttgtgat ccgggccgat cattttaaat
    15961 acggtgtcga ccgaaccaat ctcttctgca tgagccaatg gccccagcat tatcaggctt
    16021 aaagacagga tcaaatgctt gtatttcata ttgttaccat ttttatctat tacacgagca
    16081 attagttcgt cacggtaaat aacatattga ttaagaacaa aatctcaatt cttttatttc
    16141 cgtcaagccc taaaacatac tctactaaga aaaaaacact gaatgctaaa acagcaaaaa
    16201 atgctattat ccaattacct gatgtcaggt gctcgttgtt gaaaggatga ggatatttta
    16261 tggatcaggc cggcattatt cgcgaccttt taatctggct ggaaggtcat ctggatcagc
    16321 ccctgtcgct cgacaatgta gcggcgaaag caggttattc caagtggcac ttacagagaa
    16381 tgtttaaaga tgtcactggc catgctattg gcgcgtatat tcgtgctcgt cgtttgtcga
    16441 aatcggcggt cgcactacgc ctgactgcgc gtccgattct ggacatcgcg ctgcaatacc
    16501 gcttcgactc tcaacagaca tttacccgcg cattcaagaa gcagtttgcc cagactcctg
    16561 cactttaccg ccgttctcct gaatggagcg cctttggtat tcgcccgccg ctacgtctgg
    16621 gtgaattcac aatgccagag cacaaatttg tcaccctgga agatacgccg ctgattggtg
    16681 ttacccagag ctactcctgt tcgctggagc aaatctctga tttccgccat gaaatgcgtt
    16741 atcagttctg gcacgatttt ctcggcaacg cgccgaccat tccgccggtg ctctacggcc
    16801 tgaatgaaac gcgtccgagt caggataaag acgacgaaca agaggtattc tataccaccg
    16861 cgttagccca ggatcaggca gatggctatg tactgacggg gcatccggtg atgctgcagg
    16921 gcggcgaata tgtgatgttt acctatgaag gtctgggaac cggcgtgcag gagtttatcc
    16981 tgacggtata cggaacgtgc atgccaatgc tcaatctgac gcgccgtaaa ggtcaggata
    17041 ttgagcgata ctacccggca gaagacgcca aagcgggaga tcgcccaatt aatctgcgct
    17101 gtgaactgct gattcctatc cgccgttaac gctgcagctc atctaatgca ggggcgtcca
    17161 gatgcgagat gtcccctgcg gtttcaacga cccagccaga cgccagccac aggctttcct
    17221 gataatccac gcgcgaaata gaacagttac gcagacgtaa gcggcgctct acccatgctg
    17281 gtaatccgag aatcgtactc accagacatc ccagtgcaat accatggctc accagcagcg
    17341 gtcggcttcc ctgcggtaag tcccggcaag attccagtgc cgcattgacg cgatcgctga
    17401 gctcctgcat tgactcgcct tcaggaatac gcccgtcaac ggtgccattg accagctgcc
    17461 gacgccagtt ctcttcttct tcggtcagag aatcgatatg tcttttttcc agcacaccca
    17521 tgtttaattc acgcaggcga gaatcaaaga tgatgtcaca gccgcaggcc tgggcgatga
    17581 tttccgccgt acgccgggtg cgtcctaaat cgctactgat aatatgcgta atgccaagct
    17641 ctttggcacg ggttgccacc tgcatcgcct gttgctcacc tttggcggtc agcgggctgt
    17701 cagactggcc ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg cggactaggt
    17761 atacctgtaa catgcttttt ttccgtatac tgctgtaacg ttaattttct gagtaatgct
    17821 gattatgcac caagttgtct gtgcgaccac caatcccgct aaaattcagg ccattctgca
    17881 ggcatttcac gagatcttcg gcgaaggatc ctgccatatt gcatccgttg ccgtcgagag
    17941 cggtgtaccg gaacaaccct ttggcagtga ggaaacgcgc gctggcgcac gaaatcgggt
    18001 agcaaatgcc cgccgtttac ttccagaggc tgatttttgg gtggcgattg aagctggcat
    18061 tgatggcgac agcactttca gttgggtggt gattgaaaac gccagccagc gcggcgaggc
    18121 gcgttcggca actttgccgt tacctgcggt gattctggaa aaagttcgtg aaggcgaggc
    18181 gcttggtccc gtgagtcgcg ttataccggt attgatgaga ttggtcgtaa ggaaggggcg
    18241 atcggcgtgt ttaccgccgg gaaactcact cgcgccagcg tctatcacca ggcggtgatc
    18301 ctggcgctga gtccgtttca caatgccgtg tatcaggcat tgtaggacgg ataaggcgtt
    18361 cacgccgcat ccgacattcg gtgcacgatg cctgatgcga cgctgccgcg tcttatcagg
    18421 cctacaaaat caatcgcttt tcagcaacac ctcttccagc cactgacgca gctccacggg
    18481 cgcggctttc aggctgttag atccacgcgt aatcgtcgcg atgcccgcgc cgagttcatt
    18541 ttttaactca cgctgactca tttcgccacg caacagctct tcgacaatac gcacgcgagt
    18601 ccccaacgct tcgcgctcat ctggcgtcag catcaggttt aacaacggta aatggagatc
    18661 gttttggtag gcattcttaa gcaggtcgac aaaacgtaac cactcctggt gacgctgttc
    18721 tgccatcgct gctgaatagg gtgattgttg ggccataata tgtcgccatt gttagcgggg
    18781 gaagcaaaat gactcccccg gttgtactcg ctaaagagta cgatagcata tcataaacgt
    18841 gcggatcagt aacgacgtcc ccattccgtg gcgctcatca acgtcggttt atcccccatg
    18901 aaatagcggt agtaagcatc ataagccagc acgttcttca cataaccgcg cgtctcggag
    18961 aacggaatac tctcgacaaa tgccacagcg tcgatacgcc cggcgctgtt gccaagccag
    19021 gttcgcaccc gtccaggtcc ggcgttataa gctgctgagg agaaaatacg gttattgcca
    19081 aactgctgat aaacatattg caggtaactg gtgccaatgt tgatgttcgt ttccggatcc
    19141 agcaattgcc cagggctgct ataaccagga atagagaaca tcttcaccgt atgggtcgct
    19201 gtaccaggca taatctgcat caggccgctg gcccctaccg gtgatttcac tttcggattc
    19261 caggcgctct cctgacgggc aatcgccatc gcatagcttt gcgggatctc tttcccactg
    19321 gtgtaacgtt tgaaaagatc gttgtaagcc agcgggaatc gctcttccag atgatcccaa
    19381 agcttcccgg cgatcgttgc ctgaacgcta agatcccacc attggttatt gaaagcatac
    19441 cgagccagtt gagcctgctc tgtttttgac ttgctcttca ccagattggc ccactcgcta
    19501 cgcgcggtat tatcgagatt ccagtacatc aactcgcgca cgcgcgccat ctccggcccc
    19561 tgagtcaggg tgctgtcaac attctgcggc gctttatcaa tcttcagctc atactcttca
    19621 ccgatgcgtt gtgctgcaac catcgggtag aaaccacgct gttgtatgag ttgatgcaaa
    19681 atctctttcg cttcagcttc acgtccgcgt tccagcaata aatccgcctg ccagtaacgc
    19741 cattcatctt tctcttttgc ttccatcggc agacgcgcca gccaggtatt caggccgcga
    19801 cgatcgccgg tgccaagcgc cattcgcaca cggcgttcaa tcaacgacgt ggattgcgag
    19861 cgcataatgg cgtcatcgcg ccatttcgcc tgctcatcgg tgacatcatt gcccatcaaa
    19921 cgccaggcga cgatatcgcg cagctcctga atctgatctt cattaagctg ctgcgcctgg
    19981 gcaagcgacg ggatcatcag ccgtgcattt tcggcatcct gccgcgccac actggcaaac
    20041 gccaccgccg ccatttgacg ggtaaaatcg gtcgcgccgg ttgtacgcgc gaaggtcagt
    20101 accgtattag ggttgttcgc cagtgaaatg attgccgagg cgatagtctg gtaatcggca
    20161 ggcatctgcc ctgccagcac tgttaccagg cctgtgttac ccgctttcat cgccagacgg
    20221 atacgctcta aatacgccag cggatcttgt ttacctgacg cacgccagac gctaaataac
    20281 ttgtcacagg cgttaggctg gctcttgccg gttagccaca gctctttcgc cccttgccag
    20341 gcttcttcac tctgcccggt gttccatttc gcatagtagt aattacattg cgcttcggta
    20401 gttccgggct tttccgggct aaaggctaac aagccacgcc agtcttcacg ccgcgccagt
    20461 tcattgacga aacgagattg cagcgtgcga gcgggaggaa gcgtggggtt agcgcgaaca
    20521 aagttagtga ccgtcaccgc cggttgattc atcaggtcat cggtgatctg gcggtattcc
    20581 aggtagggat aaagcggata atccttcagt ccaggcatca tttgttcgac cacatccatc
    20641 tgtcgattat cccaggcctg cttgatttgc gcgtaacggc tacgctgctc atccagtgag
    20701 tcggctcgcg ccacgctgct gaccgtcagc agacagacac cggcagccaa cagccgccag
    20761 gtaacttgtt tggctttttc cacaagcgca tcctctaagt gtaaatacat caatgcagcg
    20821 tcatgccgcg taatagattt atgctaacca gtcattgccg tttacgccac gttacggaca
    20881 cttttttact tttactgcga aggtgatcga ccaactggga ttaggcgggg aaacaggcta
    20941 cacttcgcct ttgaaaactc atcatcacga taaacataga ggcgaagtcc aacgtggctc
    21001 aattcgttta taccatgcat cgtgtcggca aagttgttcc gccgaaacgt catattttga
    21061 aaaacatctc tctgagtttc ttccctgggg caaaaattgg tgtcctgggt ctgaacggcg
    21121 cgggtaagtc caccctgctg cgcattatgg cgggcattga taaagacatc gaaggtgaag
    21181 cgcgtccgca gccagacatc aagattggtt atctgccgca ggaaccgcag ctgaacccgg
    21241 aacacaccgt gcgtgagtcc attgaagaag cggtttcaga agtggttaac gccctgaaac
    21301 gcctggatga agtgtatgcg ctgtacgccg atccggatgc cgattttgac aagctggccg
    21361 ctgaacaagg ccgtctggaa gagatcattc aggctcacga cggtcataac ctgaacgtac
    21421 agctggagcg tgcggcggat gcgctgcgtc tgccagattg ggacgcgaaa atcgctaacc
    21481 tctccggtgg tgagcgtcgt cgcgtggcgt tgtgccgcct gctgctggaa aaaccagaca
    21541 tgctgctgct cgacgaaccg accaaccacc tggatgccga atccgtggcg tggctggaac
    21601 gcttcctgca cgacttcgaa ggcaccgtgg tggcgattac tcacgaccgt tacttcctcg
    21661 ataacgttgc aggctggatc ctcgaactgg accgcggtga aggtattccg tgggaaggta
    21721 actactcctc ctggctggag cagaaagatc agcgtctggc gcaggaagct tcacaagaag
    21781 cggcgcgtcg taagtcgatc gagaaagagc tggagtgggt acgtcaggga actaaaggcc
    21841 gccagtcgaa aggtaaagca cgtctggcac gctttgaaga gctgaacagc accgaatacc
    21901 agaaacgtaa cgaaaccaac gaactgttta ttccacctgg accgcgtctg ggcgataaag
    21961 tgctggaagt cagcaacctg cgtaaatcct acggcgatcg cctgctgatt gatgacctga
    22021 gcttctcgat cccgaaaggg gcgatcgtcg ggatcatcgg tccgaacggc gcgggtaaat
    22081 cgaccctgtt ccgtatgatc tccggtcagg aacagccgga cagcggcacc atcactttgg
    22141 gtgaaacggt gaaactggca tcggttgatc agttccgtga ctcaatggat aacagcaaaa
    22201 ccgtttggga agaagtttcc ggcgggctgg atattatgaa gatcggcaac accgagatgc
    22261 caagccgcgc ctacgttggc cgctttaact ttaaaggggt tgatcagggt aaacgtgttg
    22321 gcgaactttc cggtggtgag cgcggtcgtc tgcatctggc gaagctgctg caggttggcg
    22381 gcaacatgct gctgctcgac gaaccaacca acgacctgga tatcgaaacc ctgcgcgcgc
    22441 tggaaaacgc cctgctggag ttcccgggct gtgcgatggt tatctcgcac gaccgttggt
    22501 tcctcgaccg tatcgccacg cacatcctgg actaccagga tgaaggtaaa gttgagttct
    22561 tcgaaggtaa ctttactgag tacgaagagt acaaaaaacg cacgctgggc gcagacgcgc
    22621 tggagccgaa gcgtatcaag tacaagcgta ttgcgaagta atgcgtaaaa tgccggatgc
    22681 ggcgcgaacg ccttatccgg cctacaaaac cagcaatttc aatacgttgt aaaactgtag
    22741 gcctgataag acgcggtaag cgtcgcatca ggcattgagc accgaatgcc ggatgcggcg
    22801 tgaacacctt ttccggtcta caacagcctt agcatcctca atttcgtggc gggtcacccg
    22861 tgcaggtttt ttcaaacctt gtttaatctc gacagcctct tccagagagg ttttcaactc
    22921 atcaaaaaaa ctcattttat acctcgcctt tgagcagttt cgtcacggtt atccttgctc
    22981 ccccatcatc tcccgcacca gctccacgca gcgcaggaaa cggctgtcgt aatcgtcctc
    23041 ttcaacccgc acgaattcga tattgttctc ttccagcatc tccaccagca agttctggaa
    23101 ctctttgcga tccaccgaac tgccgaggct gcgtaaacca tccgccaccc acggcgtgtt
    23161 gttctccagc aggatcacca gatcgaaacg gtattcatca atcagcgcct gcacgaacgg
    23221 atgctcacgc ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat cggtgtcaat
    23281 aaacgccact ttattggcgt atttcactgc aaaatcaatg tattgtgcgt ggcccaacgc
    23341 gattttatcg tagtcggaat actgcaatgc gatctcatca ccgccgaggt gtgaaaagac
    23401 ataatcgcgg ccatattccc acgcactggt ggtgttgaag atattggcaa gtttgtttac
    23461 cagggtggat ttaccgctcg actcgccgcc aaggatcgcc acggtacgca caaaaaacgg
    23521 cttcacttcg gtaggaatat attcccagta gcggaacggg ttttcgcgga tctgcgcacc
    23581 gctgatactc ataaaggtac gtttcggatc gaccagcacc gtctcgatcc ccagatgttc
    23641 catatactgt ggcgcatcgg cttcttccga ggtgtagatc aggtccggct ggatcccctt
    23701 ttcagccata aactttttga tgccgttgct ccacacatcc cagccatgcg gatacggctc
    23761 catgccctct tcgttgaaag catgaatacg gatatttttc tgatatttaa aggtttgcaa
    23821 tagccagcgc agacgatccg gcaccgtggg ctgctgcgac atggcgctgt cttcaaacaa
    23881 cgcgcggtcg cgggtatcgt caaaacccat aatgatatgc agctcgtcaa cctggctaca
    23941 ggcgcgctgg ataaggtaga tatgtccggt atgcagcggg tagaacttac cgaatacgac
    24001 accaatggtt ttcttctgcc ggggaaattc aagccccaaa aaacggtgca atgcctccag
    24061 cttttgcgcg ctggggcttt taattttggc attcagtaac tggcttaaat accctttggt
    24121 cataccgctg gcatcagcta cctgctgtag cgtgcagccc tgttgcttga tggcagtttt
    24181 caggtaatca aatgacgaca tatctccctc cgtatctctc attataagtc gtcgaacacg
    24241 ctaagcgcgt cggagagttt tttaacgcca aaaatctgca tcccttccgg cgcttttttc
    24301 ggtacgttag ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc ttcagaaatc
    24361 cgctcctggc cactgggcac cgggcggatc tcccctgcca gcccgacttc accaaacacc
    24421 accagatcct gtggcagcgg tctgtcacgc aggctggaaa ccatcgccag cagtaacgct
    24481 aagtcgccgc tggtttcggt taccttcacg ccgccgacca cgttcacaaa cacatcctga
    24541 tcggccattt gcagaccacc gtgacggtgc aagacagcca gcaggattgc cagacggttt
    24601 tgttccagcc ccactgccac gcggcgcgga ttcgccatta tcgagtgatc gaccagcgcc
    24661 tgaatctcca ccagcaacgg acgcgttcct tcccacacca ccatcacgga gctgccggag
    24721 gtcacttcat ctccgcgact taagaaaatt gccgaagggt tgctgacttc acgcagcccc
    24781 tgttcggtca tcgcgaagac gcccagctca ttcaccgcgc cgaagcggtt tttatggctg
    24841 cgtaaggtgc gaaaacggga gtcggcatcg ccatccagaa gcaccgaaca gtcgatacag
    24901 tgttccagca ctttcgggcc agccagcgaa ccatctttgg ttacgtgtcc aaccatgaca
    24961 atcgccacac cgcgcgtttt ggcgaaacgc gtcagataag ccgccgtttc acgcacctgc
    25021 gccacgctac caggcgatga ttgtacatcc gccatatgca tcacctggat cgagtcaatt
    25081 accatcagct tcggttgctc ttcttcggca atcaggcaga tctgctcgat gctggtttcc
    25141 gacaacatat tgagattgtc agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt
    25201 tgcagcgact cttcgccggt gacatacagc gttttcatct gctgggccag tttgcacagc
    25261 gtttgcagca acagcgtgga tttccccgca ccagggttac cgccaatcag aatggcactt
    25321 cctggcacca cgccgcctcc tagtacgcgg tcgaactctt taaatccggt ggaaaaacgc
    25381 ggcagctctt caaggctgat atcggagagt ttctggactt ttgccacccc ggcgctaccg
    25441 gcatagccgc tgagacgctc gttacgcgcc accgttggcg acgcagcaag acgcacctcg
    25501 gtgatggtgt tccaggcatg acaggcactg cactgcccct gccagcgcgg ataatcggcc
    25561 ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg ccacggtgta cctcgttaat
    25621 gctgtgccgc ccgcaggatg acgggcaggc aattacttct gattcaggct gccagagagg
    25681 atgcagaata cccccatcag gtcagcgtga cggatggtga cttccgcctt ttcattcact
    25741 tttggcttgg catggtaggc aatccccagc cctgctgctt tgatcatcgg caggtcatta
    25801 gctccatcgc caatcgccac ggtctgcgcc agcgggattt catactcctg cgcgaggcga
    25861 gtcagagttt tcgctttgta ctgcgcgtct acgatgtcgc cgatcacatt gccggtaaat
    25921 ttaccgtcca tgatctccag ttcattggct accacggctg tcaggcgcag cttgtcgcgc
    25981 aggtattcag caaagaaagt aaagccgccg gaggcaatcg ccactttcca gcccagcgtt
    26041 tccagcttga gcaccagctg cgttaagcct ggcatcagcg gcagattttc acgcacctgt
    26101 tgcagaatat tggcgtcagc gcctttcagc gtcgccacgc ggctgcgcag gctggcggta
    26161 aaatcgagtt cgccgcgcat cgcccgttcg gttacctccg ccaccatctc gcccgttccg
    26221 gccagcttgg caatttcatc aatacattca atctggatgg cggtggaatc catgtccatc
    26281 accagcaaac ccggcgtgcg caggtgcggg attttcccca gcggggcgac atccagctgc
    26341 gcttcgtggg ccaggcgtgt agcccgtgcg gtgagtgaac ctgccagacg aatcacctga
    26401 taatcttcca cgcaccaggc ggcaacaatc accatcgccg cacccagttt gctctggtat
    26461 tgggtcagac gctgtttatc cagcccacga ccatacagca gccagccgct acgacctgcg
    26521 tggtaatcca gtggcatcac ttcatcacca cttaatgaaa gaggcagacc cggccataaa
    26581 gagacatctt caggcaggtc gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg
    26641 ttcgaagcag ggaaaataac gcatgaggct accttgtatc cattgcttct ggcaacatta
    26701 agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc cggctacatc
    26761 gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc actgatgcag ggggcgtcat
    26821 ggtttagtca aaaccaccag cgacagcgta atccacagct ggaagaactg gcccgcaccc
    26881 tggcgcgtca ggtgacgctg aacgttgcac cgctgatgcg taccgactca ccggatgaaa
    26941 aacgcattca ggcgatcctc gatcagttaa cagatgaaag ccgtatcctc gacgcgggtg
    27001 tgtatgacga acaaggcgat cttatcgcac gttctggcga aagcgtcgaa gtgcgcgatc
    27061 ggctggcgct cgacggtaaa aaagcaggcg gctattttaa ccagcagatt gtcgagccaa
    27121 ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac actcgacacc catacgctcg
    27181 ccaccgaagc ccaacaggtg gataacacca ctaacatttt acgcctgatg ttgctgctct
    27241 cactggcaat cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc acccgctggc
    27301 agcaatcgcc cttcctgtta accgccagca aaccggtgcc ggaagaggaa gaaagcgaga
    27361 aaaaagagtg acccattact acaagaaagg aaatcgttat gtccacatta cgactgctca
    27421 tctctgactc ttacgacccg tggtttaacc tggcggtgga agagtgtatt tttcgccaaa
    27481 tgcccgccac gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta gtaattggtc
    27541 gcgcgcagaa cccgtggaaa gagtgtaata cccggcggat ggaagaagat aacgtccgcc
    27601 tggcgcggcg cagtggcggt ggcggtggcg gcgcggtgtt ccacgatctc ggcaatacct
    27661 gctttacctt tatggctggc aagccggagt acgataaaac tctctccacg tcgattgtgc
    27721 tcaatgcgct gaacgcgctc ggcgtcagcg ccgaagcgtc cggacgtaac gatctggtgg
    27781 tgaaaaccgt cgaaggcgac cgcaaagtct caggctcggc ctatcgcgaa accaaagatc
    27841 gcggcttcca ccacggcacc ttgctactca atgccgacct cagccgcctg gcaaactatc
    27901 tcaatccgga taaaaagaaa ctggcggcga aaggcattac ttcagtgcgt tcccgcgtga
    27961 ccaacctcac cgagctgctg ccggggctca ccatgagcag gtttgcgagg ccataaccga
    28021 ggcctttttc gcccattatg gcgagcacgt ggaagcggaa atcatctccc cggacaaaac
    28081 gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag agtagctgag agtggaactt
    28141 cggtcaggct ccggcattct cgcatctgct ggatgaacgc tttagctggg gcggcgtgga
    28201 actgcatttc gacgttgaaa aaggccatat cacccgcgcc caggtattta ccgacagcct
    28261 caacctcgcg ccgctggaag ccctcgccgg gcgactgcaa ggctgcctgt accgcgcgga
    28321 tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc ccggaccagg aaaaagagct
    28381 acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa ctcgctgatt aaaatatcca
    28441 aggaaggaaa tatgatcgat ctggaaaacc aggaacggga gattatcaat ttaatgttca
    28501 gccagcgtat ttcctggcta gccgccgtgc gtatcaggca taaattatcg cttgctgaag
    28561 taagtaaaat gttaggtatc tcgattaatt ctctaaaaca gatcgaaaag acggaacggc
    28621 ttagctcaaa catcaaaagc aaaatggcag aaatttatgg ttgtccacca gagttgctga
    28681 tatgcccttc ctggatgacg gctgaacata aatgaataga ttcgtgaata gatacttgaa
    28741 tgttttttat tttctatcca tatgaaaaat aatagtaaat ctataaatca gttgctattg
    28801 atgaatagat actaaaaagc cggagcagtc ccccactccg gcttaacacg acaatcgcct
    28861 ttcagcgaaa cacaattact ctttatcgcc cagcagaacg gattccagtg cgattttgat
    28921 catgtcgttg aaggtggtct gacgctcagc ggcagtggtc tgctcgtgag tgcggatgtg
    28981 gtcagacacg gtgcagatgg tcagggcttt cgcgccaaac tctgcagcca cgccgtagat
    29041 accagccgct tccatttcca cgccgaggat gccgtatttt tccatcacgt cgaacatttc
    29101 gccgtccgga gagtagaaca ggtcagcgga gaacaggtta cccacgcgag catcaacgcc
    29161 cagtgcttta gctgcatcta ctgcgttacg caccatgtca aagtcagcga tagcggcaaa
    29221 gtcatggtct ttaaaacgga tgcggttaac tttggaatcg gtgcaggccc ccataccgat
    29281 aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca caggaaccaa cgcggataat
    29341 tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag atggagcagg acgggatacc
    29401 cataccgtga cccattacgg aaattttgcg gcctttgtaa gtaccagtga agcccagcat
    29461 accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa gtttcagcaa tatacttcgc
    29521 acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa tcgcccattt ctgcattaat
    29581 gtgtggggta gccattgttt tatccttttt gtgacataac aaaggcggag tcaacgctcc
    29641 gcccaaatcc atcagaacat ggctttgcca tattccatat cagaagtacc aaaatatttt
    29701 gccagagtct ggccgatatc cgcgaaggtt tcacggtgac ccagtgagcc cggttttact
    29761 ttcgggccat ataccagtac cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc
    29821 ggatcgcaac cgtggtcagc ggtgaggatc aggatgtcgt catcgcgcag cagagacatc
    29881 agctccggca gacggcggtc gaacagttcc agacccgcag cataaccggc gacgtcgcgg
    29941 cggtggcccc aggaagagtc gaagtcaacg aagttggtga agacgatggt gttatcaccc
    30001 gcttctttca tctctttgat ggtggcgtca aacagcgcgt ccaagccagt cgctttcact
    30061 tttttggtga taccgcagtt ggcgtagatg tctgcaattt taccgacaga aaccacctgg
    30121 ccgtgttttt catcaaccag tttctgcagc acggtcggtg ctggcggctc aacagccaag
    30181 tcgtgacggt taccggtacg ctggaagtta ccagctttgt caccgataaa cggacgagcg
    30241 ataacacgac cgatattgta gccgccgttg gtcagctctt cacgggcgat ttcgcacagt
    30301 tcgtagagtt tatccagacc gaaagtttct tcatggcagg caatctggaa cacggagtca
    30361 gcggaggtat agaaaatcgg cttgccggtt ttcatgtgct cttcgcccag ttgatccaga
    30421 atgaccgtac cggaagagtg gcagttaccg aggtaacccg gcagattagc gcgttcgacc
    30481 agtttatcca gcagctcttg cgggaagctg ttttcgtgat cggagaaata tccccactca
    30541 aacagaaccg ggacaccggc gatttcccag tgaccagacg gggtatcttt accggatgac
    30601 atttcgtgcg cccatgcgta cgcgccgata acttcagcgt tgccgtccat tcccgccgga
    30661 atgaaaccgg tagaaccttc gtgtgctttc gccagcccca gacgggtcag atttggcaga
    30721 ttgagcggcc ctttacgacc attatcagct tcgcctttgg cacaagcttc tgcgatatga
    30781 cccagggtgt cagccccgac gtcaccaaag cgttctgcat cttctgtagc gccgatgccg
    30841 aatgagtcca gcaccataat aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa
    30901 atttaaatga tcagatcagt ataccgttat tcgctgatac ggcgatagac agttggtgtg
    30961 ctttccggtg cgttatcggc aagtttaatt gccgctttca ccgctttcgc cgcttcctgc
    31021 cagctgtttt cgtctttcgc gaggataacc gccagcggac gctgaccgtc tacctggtcg
    31081 cccagacgcg ccatatcagt aaagccgacg ctgtaatcga tggtgtcaga tgcctgacga
    31141 cgaccgccgc ccattgcaac cactgccatc cccagcgcgc gggtatccat ttcactgaca
    31201 aaaccttcgg tatcagcata gactgctttc gtcagcatcg ctgtcggcag atacttcgcg
    31261 tagttctcaa cgaagtcggt cgggcctttt tgtgccgcta ccatacgacc aaagacttct
    31321 gccgctttac cgttgtccag caccgcctgc aatttcgcgc gcgcttcggc gtcatctttc
    31381 gccagtttgc cggagatcag catctccacg cacagcgcca tcgtgacatc aaacagacgc
    31441 gggttacgat actcacccgt caggaactgc accgcttcac gaacttcaac cgcgttacct
    31501 gcactggagg ccagtacctg attcatgtcg gtgagcagcg cggtggtgcg cacgccagcg
    31561 ccgttagcca cgccaacaat cgcttcggca agggcttcag agagttcgta ggtcggcata
    31621 aacgcgccgc tacccacttt cacgtccatc accagcgcgt ccagaccttc cgccagtttc
    31681 ttcgccagga tcgaggcagt gatcagcggg atggagtcca cggttgcggt aatatcacgg
    31741 gtcgcgtaga aacgtttatc agccggagcc agtgaactgg tctgaccgat aatcgccacg
    31801 ccgacgtctt taataatttc gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg
    31861 atggattcca gtttgtcgag cgtaccgcca gtatgaccga ggccgcgacc ggagatcatc
    31921 ggaatatagc cgccgcaggc tgcgaccatc ggccccaaca tcagcgaagt gacatcgccg
    31981 acgccgccgg tcgagtgttt atcaacaatc gggccattca gatgcaggct tttccagtcg
    32041 agaacggttc ctgaatctcg catcgccatg gtcagcgaga cacgctcagg cattgtcata
    32101 tcgtggaaga aaatggtcat cgcgagggcg gcaatctgcc cttcggaaat agtgttgtcg
    32161 cgaataccgt tgataaagaa acgaatttct tcatcgctca gcgcatgacc atcacgtttt
    32221 ttacgaataa tttcttgtgc gagaaacaag gtaacctccc gaggaaaaga gtatgaggcg
    32281 gcttttgccg gatgcgtcta cgccttatcc ggcctacggg tagatacgtc tcgtaggcct
    32341 gataagcgca gcgcatcagg cgtaaagcat cttacttagt agctgctggc gctcttaccg
    32401 tcgccgtggc ccagcgcttt cagcaggctt gccagcaggc tggaagcacc aaagcggtag
    32461 tgacgcgcat cagcccagtc agcaccgaac agttcatctg cgatggcgag atatttctgc
    32521 gcatcttccg cagtacgcac gccgcccgcc ggtttgaaac caacggtttt ttctacgccc
    32581 atatcacgga tcacttccat catgatgcga gcgctttccg gcgtcgcgtt cacagccact
    32641 ttaccggtag aggttttgat gaagtccgca cccgctttga tggagatttc agacgcttta
    32701 cggatcagcg cttcgtcttt cagttcgccg gtttcgatga tcactttcag cagtacattc
    32761 gctgccgcgc aagcctcttt acaggctttc accaggtcaa aaccaacctg ctcgtcaccc
    32821 gccatcagcg cgcggtacgg gaacaccacg tcaacttcat cagcaccgta ggcgattgcc
    32881 gcacgggttt ctgccagcgc gatttcgatg tcgtcgttac cgtgtgggaa gttggttacc
    32941 gtagcgatac ggatttccgg ggtgccctgc tctttcagag ttttgcgagc aatcgggata
    33001 aaacgaggat agatacagat agcggcggta ttgccgaccg gagttttggc ctgatgacac
    33061 agagcaatta ctttctcgtc ggtgtcgtcg tcattcaggg tggtcaggtc catcaatttc
    33121 agtgcacgca ggctgcttgc tttcagatca gtcatttcat tctcctggct tgtcgccaat
    33181 aaaattcacc ttgcgagttt gttagtattc taacatctac tccgcaacac acttcgatac
    33241 acatcacaat taaggaaatc tacttacaag tttgcatcac tgtaatgcga tctggttcaa
    33301 ataattcact ttcaaatgaa tgcgtcagtg gtggcaaacg catcaggatc aaagtgaaca
    33361 tcacgaaact tcttacaatg gcgcatcacc ttcggcataa aaggaaaaga agatgcccac
    33421 ttctcatgaa aatgcactgc aacaacgttg ccagcaaatt gtcaccagcc cagtgcttag
    33481 cccggagcag aagcgccatt ttctggcact ggaagcagaa aacaatctgc cttacccaca
    33541 gcttcctgcc gaagcccgcc gcgcgctgga tgaaggtgta atctgcgata tgtttgaagg
    33601 tcatgcgccg tacaaaccgc gctatgtctt acccgattac gctcgttttc tggcgaacgg
    33661 ttccgaatgg ctggagctgg aaggcgcgaa agatcttgat gacgcactct ctctgctgac
    33721 cattctttac caccacgtac cgtcggtcac atcgatgccg gtctacctgg ggcaactgga
    33781 tgcgttgttg caaccgtatg ttggaattct aacacaaaac gagatcgatg ttcgaataaa
    33841 acgtttctgg cgttacctcg acagaaccct gccagatgcc tttatgcacg ccaatatcgg
    33901 cccgtctgat tcgcccatta cccgcgcgat cttacgtgca gacgcgcagg tttcaccgaa
    33961 cctgaccttt atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa
    34021 gaacatctgt gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt
    34081 cacaaaaggg ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg
    34141 cagcacgctg gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga
    34201 cttctttacg cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg
    34261 gtgtgaattc ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg
    34321 gctgattaac cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt
    34381 taacttgctg tgtgagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa
    34441 tgaagtgggt tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa
    34501 atatggctgg caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg
    34561 caccacgccg ggcgcgcgtt tgccgtatgg cgatgagcca gatccgatca cccatctgca
    34621 aactgtcgcc ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga
    34681 cgaaaccatc aaacgtaacc cgcaggcact ggtacagctt tgcctcggtg cctttaaagc
    34741 cggaatgcgt gaatttaccg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta
    34801 tatggtgcgt ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac
    34861 ctggctgggc gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat
    34921 aagccatgaa cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg
    34981 ggccaggcag tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc
    35041 acaatccgtg gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc
    35101 atcaggcgtt gcagattgtt gacggcaaag tggtgtggaa cgctgtggtc tgcgagcaat
    35161 gtgatacctg cctgaagatg tgtccgcaac atgccacacc aatggcgcaa tccatgagcg
    35221 tggacgaagt gcttagccat gtgcgcaaag cggtgctgtt tattgaaggg ataacggtaa
    35281 gcggcggcga agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa
    35341 acgatccgca actgcgccat ctcacctgtc tggtggacag taacggtatg ttgagcgaaa
    35401 ccggctggga aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcatggg
    35461 gtagcgaatg tcatcaacaa ttaaccggac gcgataatca acagattaag cgcagcatct
    35521 atttgctggc agagcgcggc aagctggcgg aactgcgttt gctgatgatc ccaggccagg
    35581 tggattattt gcaacacatc gaagaactgg cggcgtttat caagggactt ggtgatgttc
    35641 cggttcgcct gaacgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga
    35701 gcgccacgcc ggaagacgtt gagccgttgg ctgatgcatt aaaggtgcgc ggggtgagcc
    35761 ggttgatatt tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag
    35821 acttgtaggc ctgataagac gcgtcaagcg tcgcatcagg cattgtgcgc caactgccgg
    35881 atgcggcgtg aacgccttat ccggcctacg gttcggcaca aacctgtagg catgataaga
    35941 cgcgttaagc gtcgcatcag gcattatgcg ccgactgccg gatgctgcct acgaagggca
    36001 aactacccac gaacgttaaa caccgcatac gtgttattaa gcaacacttc ggcaatctca
    36061 tccgcgggtt ccgggcgtaa ctcgcaaagc acggcaaaca cacgggcagc ctgttccggg
    36121 cggttaggct gcccctgaaa accgttgagc ggcatatccg gcgcatcggt ttccagcaac
    36181 aacgatgcca gcggtaattt tgcgatgaca tcgcgggttt tactggcgcg tggataggtg
    36241 atagtaccgc ctacgccgat tttgtagccc agctgtacaa accgttcagc ctgttgctgg
    36301 ctgccagaaa atccgtggac aacaccagca cgcggcaaat cgtggtgttt aagatgcatc
    36361 gccagtttgt cgtgcgtgcg ccgtgaatgc aggatcaccg gcagatcgta gcgtttcgcc
    36421 agtttcagtt gttcgtcgag taaccactgc tgcctctcaa attgcggatc gtcgccgaag
    36481 agatccagac cgatctcccc caccgccacc accttcgccg gacgcctttc cagcgcctgc
    36541 tgtagctgct caagagacac atcgctatgt ttttccaaca taccaggatg caagcccaat
    36601 gcggcataca gcggttgata attttccgct aacgcctgca cgcgggcaaa attttccgcc
    36661 tctgtcgccg gaacaatgat cttgcctacg cccgcttgtg ccgcgcgttg caggctggcc
    36721 tcttcatcgc caataaaagg cgggaaatca aaatggcagt gagtgtcgat aaaacggcaa
    36781 atcaagccag atcctcattg ttaaatgtcg ggtcgttcgc ctgcggagcg tcgctcactt
    36841 cggcaaccag cgtatcgttg gcgactggcg caggcggaat gacaatcgat tccggcgtca
    36901 ccactggcac cagatggcgg gtaagcggcg ctttttcagt taatagcttg ccaaccgtgg
    36961 cgaggaaata acgaccgcat aaacgcccaa gtttatagtc ttcacgcagc gccggaatcc
    37021 gactgccaag cgcgatacta tgtaatggct tcggcggata aatttcggat atccgcagct
    37081 tgcccggtgg tttctcaata aattgctgaa tgtcacgata gctggtttca tgatgctgca
    37141 ccagattgac cagcggctgc aggctactgt cacccagcca gcgttccatg cgtttgaacc
    37201 actgcggcgt gtagtacatt tgtgacggca cagtgcggat gacgaccaac gttttagcgc
    37261 cctgccttgc cgcctcttta accggaatcg catcactgat cccgccatcc aggtagttaa
    37321 tgccttccag cgacactccg ctacgataaa agccaggtat cgcactggag gcgcgaatca
    37381 catccagcca gttttgtttg gttggtaaaa agtaattcgg ctcgtagtca tcctgacgac
    37441 aggcgcacat ataaaacgat ttgccgctgt caaacaaccg cgcggcggta tccatttgca
    37501 acggcatctg gcttgcggtg gcctccacca gccagtcgag atcgataaga tttcctccac
    37561 ggacaaagcg caatggatcg aaaaattcgc gttttgtggt atagcgcatg atgactttgc
    37621 gcgcgtaacc gggctgattg cagataaacg ccgagaggtt ctgcgccccg gcagatgtgc
    37681 cgagataaag atcgaaagga ttaaactgcg cgcgcataaa ctcatccagc acgccagccg
    37741 tgaaaattcc acgctgtccg ccgccttcgc acaccagagc tattcgtcca ggctggaacg
    37801 gccttagcga caacggcgca atattaccaa gcgttacagg tattcgctgc cccacttctg
    37861 ccttcctgtt tttgttgtga aagaaagaca aagtaacgca atttattatt cgctaaaacc
    37921 gcaaaagcca gtccgctgga ctggctttgg ataaaatttt cttaatggtc tgaaatctag
    37981 ggtcgttttc ggcccatgaa caaactcact aggaacagaa taatcccgac gacaaagaca
    38041 attttagctg cgcctgcagc ggtaccggcc agaccaccaa acccaagtgc ggcggcgatt
    38101 aacgcgataa ccagaaatat gatgccccaa cgaaacataa gcgtctcctt taccatagtt
    38161 aatgtcaccg cttgatatga gcgtgaaaat cactcaatgc gatattttta gtgtggtgca
    38221 catcgcgcct cccgacaaag ttcgggagga cgaattactt agttttcaga tcatttttaa
    38281 cgcttttcac accatctacc gctttggcga tactttcagc acggtcactt tgtgcctgag
    38341 aatctacggt accggagagc tgaaccacgc catcggtggt ttcaactttc actttacgtg
    38401 aagggacgat atcgtccgcc agcagtttgg ctttgatttc actggtggtg gcggtgtcac
    38461 ccgcgtagcc cttcaccgag ccttctttag cgtcgcgaac gtgcagtttg tcgctgacag
    38521 aggtcacgcc ttcaacgcct ttcgccactt tcactgcctc ttcggcctgg gcctgacttt
    38581 caacgaaacc gctcagggtc acgacttttt gatcggtttt gacagagata tcggtgctct
    38641 tgatgttgtc atgatccacc agcgccgcct tcactttcgc ggtgatggcg ctgtcatcca
    38701 tgaaattacc gactttattc atagagctat cgactttttg ccctgcgctt tcattggtag
    38761 tctgcgcgtt gttttccgcg taggcagagc cggtcgcgac ggcagaggtc aacattacag
    38821 ccagcagagt tttcgaaatc ttcagtcttg tcatagtcat cgatttattc ctgtatgttt
    38881 gctcgtaatt tgagcctagc aacacgaggt tgcattgctg aatagggaga gacttcaccc
    38941 tctacagaag ccaatggtcg ccatcacaaa agcgatgggt gatgaataac gaccattaca
    39001 gcctctgaat cagttattaa tatcggtaga atgacaatcg acggctttag atactgatat
    39061 ctacgcattg aacggtattt aacgccatca gaaatgtcat cactttgtta aatatagatc
    39121 acaattttga aaccgctcgg gatatcagcg aaaacataag caaaagtgaa tgttttaaga
    39181 acattccgta agcggctaat aaggaaggga aattgacagg gcgcagcggc taccgcgccc
    39241 tggaggcaag aattaatgct cgcgggtctg gtggaactga acgtccggat aacgttcctg
    39301 tgccaggcgc aggttgacca tgctggtagc gatgtaagcg aggttatcgc cgccatcaag
    39361 cgccagttgg ctttcgttct tacgcttgaa ctcttcgaat ttcttcgcgt ctgcacattc
    39421 tacccagcgg gcagtggcaa cgttgactga ctcatacact gcttcaacgt tgtattcgct
    39481 cttcaggcgc gctaccacca catcaaactg cagcacacca accgcaccaa cgatcagatc
    39541 gttgttggag attggacgga acacctgcac cgcgccctct tcggaaagct gtaccagccc
    39601 tttgagcagc tgtttttgct tcagcggatc tttcaggcgg atacgacgga acagttctgg
    39661 tgcgaagttc ggaataccgg tgaacttcat catctcaccc tgggtaaagg tgtcgccgat
    39721 ctgaatggtg ccgtggttgt gcaggccgag gatatcgccc ggatacgctt cttcaacgtg
    39781 cgaacggtca cccgccataa aggtcagcgc gtcggagatc accacatctt tcgcagtgcg
    39841 cacctggcgc agtttcatgc ctttttcata tttaccggac accacacgca taaacgccac
    39901 gcggtcgcgg tgtttcggat ccatgttggc ctgaatttta aatacgaagc cggtaaattt
    39961 atcttcgctc gcttctacgg tacgggtatc ggtctgacgc ggcatcggcg caggcgccca
    40021 ctccaccagg ccattcagca tatgatcaac gccgaagtta cccagcgcag taccgaagaa
    40081 taccggagtg atttcgcccg caaggaacag ctctttgtcg aactcgttag acgcgccttt
    40141 aaccagttcc agttcgtcac gcagctgctg tgccagatct tcaccaaccg cagcatcgag
    40201 atccgggtta ttcagccctt taacaatgcg gacttcctga atggtgtgcc ctttaccgct
    40261 ctgatagaga taggtttcat ctttataaag gtggtaaacg cctttaaaca gcttgccgca
    40321 gccaattggc caagtgatcg gtgcgcagcc aattttcagc tcgttctcaa cttcatcgag
    40381 cagttccatc gggtcgcgga tatcacggtc aagtttgttc ataaaggtga ggatcggcgt
    40441 gtcgcgcaga cgggtaactt ccatcagctt acgggtacga tcttcaacac cttttgcggc
    40501 gtcgataacc atcaggcagc agtccaccgc cgtcagggta cgataggtat cttccgagaa
    40561 gtcttcgtgc cccggggtgt cgagcaggtt aaccaggcaa tcgtgatacg gaaactgcat
    40621 cacagacgta gtaatggaga tcccacgctg cttttccatc tccatccagt ccgacttagc
    40681 gtgctggttg gaaccacggc cttttactgt accggcggtc tgaatggcct gtccgaacag
    40741 cagcaccttc tcggtgatgg tagttttacc ggcgtccggg tgagaaataa tggcaaaagt
    40801 gcggcgcttc gccacctctt tcaaataagg agacaacgtc ataattcttt cttcttgagt
    40861 aagcggcatc gcgccgcgct tgttgaaaac gaaaaattgc ggctatttta cccatcaacg
    40921 ggggggaggc aatcagtgtt tacacaggag ctgctccagt tcgtgcaacg aagaaacggt
    40981 ccaggtgggc gcgatgcctt ctggttgctc gcgattgtgc gcattcagcc agcaggtcgc
    41041 aagcccggcg ttgatgccac cgagaatatc ggactcggca gtgtcgccaa ccatcagcac
    41101 gcgtgaacgg tcaggattgc ccgcctgttc cagcgcataa tcgaaaattt tcttattcgg
    41161 tttggcaacg ccaacttctt cggaaatcac cagcaaatcg aagtaatcac gcaggcccgt
    41221 ccgttccaga cgcacttgct gcaaggcgct aaaaccgttg gtgatgatgc cgattttagc
    41281 gttgctacga atggcgttaa gcagagaaac cgcgcccggc aacggcgtac agatttccgc
    41341 catcgcatta ataaaggcat cgttaagcaa gcctgcttca actttcagcc gttcggccca
    41401 gctctcgaaa cgcccgtgct gaagctgtaa tgaagtgatc gcgccgtttt gataatccac
    41461 ccacagtggc ttgttaacgg cctgatagtc ctgaaaatct tcagcggtaa aggtgacgct
    41521 gtaatcaaga aacatccgct gcaggccggt gaatgagtca aaggtaaaca gcgtttcatc
    41581 ggcatcaaag aaaatccagt cccacttcat tattccacct tgtcttacat actgattggc
    41641 aacgccatga tgatggcgtc ttcgcgaccg tccgtggtgg ggtagtaatt gcggcgaatc
    41701 gtcgcctcgt taaagcctaa actttcgtac agggcaatgg cggcagcatt tgaggcacgg
    41761 acttccagcc atagtgtcgc cacgccgcgt ttttccagtt cgtcgatcag atgttccagc
    41821 agcgccctac ccagcccctg acgctgatag tcgggatcga ccgcaatatt gaacaatgta
    41881 gcttcatcca gcaccacttg cgtaatcgca aacgccgcca ttttgccgtt ttgcgttaac
    41941 tgaaagttga gataacgctc gccctggttg ctggcaaagg ttttttcact ccacggaaag
    42001 gagtgggcgc gttgttcaat gtggtaagcc gctggtaaat cagtcgtttc gagggaagaa
    42061 atcgtgttca tatgtgcaaa tttgttgcca taacgcggcg cgtgccgttg ggtttgcccg
    42121 taattcggtg agcgccggtg atgccacctg agcgccttcc aatgatagcg gttcgtcagt
    42181 acccaaccgc cagctgttgc agcgactgcc ttgcggcagc atcgcgattt tttctggcgt
    42241 cagttgcagc acttgatcgg ggctgacggt taatgcgcgc agaacatcgc tcactaaagg
    42301 atcagtcagg gcgggaagat cgtttgccac catcaccaga cggacgtgtg ccgggctcgc
    42361 aatggcaatc tccccctgca acgcgccagg gcgacgcagc gaccactggg taatgcccag
    42421 ttgctgtaac tgccagtctc gtcgggatgt catagcgaat cgctcctgtt gtcaggggcg
    42481 caaatatagc aaattcgtcg ataccgcgcc aacatatggc tataatcgcc gccagtatca
    42541 attgaggagc attccatgtc tgcatttacc ccggcaagtg aagtcttgct gcgtcacagt
    42601 gatgatttcg aacaaagccg tattctgttt gccggagact tacaggatga cctgcccgcg
    42661 cgtttagata ccgcggccag ccgtgctcat acccagcaat tccaccattg gcaggtgtta
    42721 agctgccaga tgggggataa cgcccgtttt agtctggtcg ccacggcgaa tgatgtcgca
    42781 gattgcgata cgctgattta ctactggccg aagaacaaac cggaagccca gttccagttg
    42841 atgaatttac tttctctgct gccagtgggg acagatattt ttgtcgttgg cgagaaccgc
    42901 agcggcgtgc gcagcgctga gcagatgctg gcagattatg cgccgttgaa taaagtcgac
    42961 agcgctcgtc gctgtggcct ctattttggt cgtctggaaa aacagccagt atttgatgcg
    43021 gataaattct ggggcgaata cagcgtcgat ggcctgacgg tcaaaacgct gcctggcgtg
    43081 tttagccgcg acggtctgga tgtcggtagc cagttgctgc tctcgacgtt aactccgcac
    43141 acgaaaggta aagtgctgga tgtcggctgt ggcgcgggtg tactttcagt tgcctttgcg
    43201 cgccattcgc cgaaaattcg tctcaccttg tgcgatgtct ctgccccggc ggtagaagcc
    43261 agccgcgcaa cacttgcagc caacggtgtt gaaggtgaag tctttgccag caacgtcttt
    43321 tccgaggtga aaggttgttt tgatatgatc atctccaacc cgccgttcca cgatgggatg
    43381 caaaccagcc tggatgcggc gcaaacgctg attcgtggcg cggtgcgtca tcttaatagc
    43441 ggcggcgagc tgcgaattgt agcgaacgcc ttcctgcctt acccggacgt gctggatgag
    43501 acatttggct tccatgaagt tatcgcgcaa accgggcgct tcaaggtata tcgcgccatt
    43561 atgacccgcc aggcgaagaa aggttgagta tctgatatct gtcgccgcat accgtgtcgg
    43621 atgcggcgtg aacgccttat ccgacctaca aaacctgcac gttagccctt cgtaggccag
    43681 ataagacgcg ccagcgtcgc atctggcatc tgcccccgca tgcctaccaa acctgcacgc
    43741 tagtttcctg atggacattt ttccagcaat tacacatcag gcgataatta actattgacg
    43801 aaaagctgaa aaccactaga atgcgcctcc gtggtagcaa ttctttttaa gaattgatgg
    43861 tatgcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt ccttacggac
    43921 gtgggggttc aagtcccccc cctcgcacca aaaaccacgt tgatattgct cgcactgggc
    43981 gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgttctta cggacgtggg
    44041 ggttcaagtc ccccccctcg caccaaacga ggcgatatca aaaaaagtaa gatgactgtg
    44101 cgaaggtggc ggaattggta gacgcgctag cttcaggtgt tagtgtcctt acggacgtgg
    44161 gggttcaagt cccccccctc gcaccaatta tctttacttc ctttcttgtt tcttccctgt
    44221 tttgtcatcc ctgattcaaa ttcattatca tcagcatgat gccagcaata agcgccaggc
    44281 acgatggtag caaaatgtaa tgccgcaact gtttgtgata cagcatcacc ggcgtcaata
    44341 ctaatcccat cagaatgaca atccgccact gatccggcga taaatccgca tacatcagcc
    44401 ctgcaatcag caatcccggc agcaacactc cccagccact gcccagcgta cgttgcaaca
    44461 tggtttcatc tctttcattg ataatgaaaa ccaatatcat atgataattt ttatcatttg
    44521 caagccagag aaatcccttg ctatcgagta aacctatcgc taggattagc aatcattatc
    44581 atttagatta ctatcacgat tatggcctat cgttccgcac cgctctatga agatgtcatc
    44641 tggcgaacgc atctccagcc gcaggatgct gggcttgcac aagccgtacg ggcgatgatc
    44701 gccaaacatc gtgaacattt gctggagttt atccgcctgg atgaacccgc tccgcttaac
    44761 gccatgacgc tggcgcaatg gtcatcaccg aatgcgctaa gttctctact ggcggtctat
    44821 tccgatcata tctatcgcaa ccaaccaacg atgatccgcg agaacaaacc gctgatctca
    44881 ctatgggcac aatggtatat cggcctgatg gtgccaccat taatgctggc gctactgacg
    44941 caggaaaaag cattagatgt gtcgccggaa catttccatg ctgagtttca cgaaaccgga
    45001 cgcgtcgcct gtttctgggt cgatgtgtgt gaagataaaa acgcaacacc acattcgccg
    45061 cagcagcgaa tggaaacgtt aatcagccag gcgctggttc cggttgtgca agcactagaa
    45121 gcgaccggag agatcaacgg caaacttatc tggagtaata ccggttattt gatcaactgg
    45181 tatctcactg agatgaagca actgctcggc gaggctactg ttgaatcgct gcgccatgcc
    45241 ctcttttttg agaaaacgct cacgaacggt gaagataatc cactctggcg taccgtggta
    45301 ctgcgcgacg gcctgctggt gcgccgcact tgctgccagc gttatcgctt accggatgtg
    45361 cagcaatgtg gctattgtac gctgaaatga aagcactgcc ggggaagtaa acccggcatc
    45421 atgcggatta atagggatgc aacacattac ttgcttcgca atgccgcata cataacagaa
    45481 tatctgcggc ctccaacaac cctgcatcag aactgacgcc cagtttcgac atcacattaa
    45541 atttatgcgc tcggatcgtt ttgatattac gtttaagctg ctcggcaatt tgtgtcattg
    45601 agtagccacg cgacataaag cgcaatattt ctctctccgt cgggcttaac gtccggcttt
    45661 ggttaatgta ccactgattg ttcagtcggt cggtcgcctg acgtacacca tttaatgaca
    45721 aaaagagttc ctgatgaaaa atctccagcg ttgacgcttt acttaataca ccgtccagcg
    45781 gtgatggcga caatgaacca atcagccgag cttcaatatc atcatccaca ataactaaac
    45841 gccgggtacg cggaaactta atcgccagct cagtcaggca agataatccc tctctgcgct
    45901 cacttctcat ggcagaaaaa gaaaaaatga ccgccgcaaa cgagatccgc gacatggcat
    45961 cctgaaatga ctcctgactg gtataggtat ggagcttata agggttgccc gcaaactttc
    46021 tggataaact ctcaataccg atactactca tgacgcattt ttctatgagt gcgacatttc
    46081 tcttcttaat tcggctgtgt tccattctgc ggcatctcca taaacagaag gcaagttaag
    46141 ttctcttgca ccctgcgcgc tatttatcca tgcatacatt tcagcgttgc tgtgaattgc
    46201 cagtcgacgc atcgcactgt ttttttgggc gctgatagtt ttattacttt tcttaagcaa
    46261 tgaagctatc tggttgatgc cccatccttt acctaatagc cgcagtacct tgcgctctga
    46321 aagagtgaga atgacggagc gatagctaaa atcatgagtt tcagtcattg ccggagaggt
    46381 cagcatggcg ctgatacgct ctgcgtgcgt attgccggaa cgtacggtct tgaccagatt
    46441 ctcaatgggt tcaacatcag acaataacgt cgccgtaggg cacatgagca gttctacagc
    46501 ctgggaatac caggagcgtg acaccatgaa aacccagtga atttcccgat attgtgagat
    46561 taaagaataa taatgttcac aaacagaacg tggatcttca ctttcaccgg ctaaatcagc
    46621 aatgactaat
//
LOCUS       DAECNO010000022        46535 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.022, whole genome shotgun sequence.
ACCESSION   DAECNO010000022 DAECNO010000000
VERSION     DAECNO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.022"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(75..1934)
                     /gene="gss"
                     /locus_tag="KKO08_001237"
     CDS             complement(75..1934)
                     /gene="gss"
                     /locus_tag="KKO08_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBD6317558.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2139..3005
                     /gene="yghU"
                     /locus_tag="KKO08_001238"
     CDS             2139..3005
                     /gene="yghU"
                     /locus_tag="KKO08_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBD6317559.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3128..3376)
                     /gene="hybG"
                     /locus_tag="KKO08_001239"
     CDS             complement(3128..3376)
                     /gene="hybG"
                     /locus_tag="KKO08_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBD6317560.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3510..3851)
                     /gene="hypA"
                     /locus_tag="KKO08_001240"
     CDS             complement(3510..3851)
                     /gene="hypA"
                     /locus_tag="KKO08_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD6317561.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3844..4332)
                     /gene="hybE"
                     /locus_tag="KKO08_001241"
     CDS             complement(3844..4332)
                     /gene="hybE"
                     /locus_tag="KKO08_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBD6317562.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(4325..4819)
                     /locus_tag="KKO08_001242"
     CDS             complement(4325..4819)
                     /locus_tag="KKO08_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBD6317563.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4819..6522)
                     /gene="hybC"
                     /locus_tag="KKO08_001243"
     CDS             complement(4819..6522)
                     /gene="hybC"
                     /locus_tag="KKO08_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBD6317564.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6519..7697)
                     /gene="hybB"
                     /locus_tag="KKO08_001244"
     CDS             complement(6519..7697)
                     /gene="hybB"
                     /locus_tag="KKO08_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBD6317565.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7687..8673)
                     /gene="hybA"
                     /locus_tag="KKO08_001245"
     CDS             complement(7687..8673)
                     /gene="hybA"
                     /locus_tag="KKO08_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBD6317566.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8676..9794)
                     /gene="hybO"
                     /locus_tag="KKO08_001246"
     CDS             complement(8676..9794)
                     /gene="hybO"
                     /locus_tag="KKO08_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBD6317567.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9983..10270)
                     /locus_tag="KKO08_001247"
     CDS             complement(9983..10270)
                     /locus_tag="KKO08_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBD6317568.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(10389..11276)
                     /locus_tag="KKO08_001248"
     CDS             complement(10389..11276)
                     /locus_tag="KKO08_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD6317569.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11433..12473
                     /gene="gpr"
                     /locus_tag="KKO08_001249"
     CDS             11433..12473
                     /gene="gpr"
                     /locus_tag="KKO08_001249"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBD6317570.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12513..13007)
                     /locus_tag="KKO08_001250"
     CDS             complement(12513..13007)
                     /locus_tag="KKO08_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBD6317571.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13198..14082
                     /gene="yghA"
                     /locus_tag="KKO08_001251"
     CDS             13198..14082
                     /gene="yghA"
                     /locus_tag="KKO08_001251"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBD6317572.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14354..14779)
                     /gene="exbD"
                     /locus_tag="KKO08_001252"
     CDS             complement(14354..14779)
                     /gene="exbD"
                     /locus_tag="KKO08_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBD6317573.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14786..15520)
                     /gene="exbB"
                     /locus_tag="KKO08_001253"
     CDS             complement(14786..15520)
                     /gene="exbB"
                     /locus_tag="KKO08_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD6317574.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15772..16959
                     /gene="metC"
                     /locus_tag="KKO08_001254"
     CDS             15772..16959
                     /gene="metC"
                     /locus_tag="KKO08_001254"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBD6317575.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17099..17758
                     /locus_tag="KKO08_001255"
     CDS             17099..17758
                     /locus_tag="KKO08_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6317576.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17798..18697)
                     /gene="yqhC"
                     /locus_tag="KKO08_001256"
     CDS             complement(17798..18697)
                     /gene="yqhC"
                     /locus_tag="KKO08_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBD6317577.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18891..20054
                     /gene="yqhD"
                     /locus_tag="KKO08_001257"
     CDS             18891..20054
                     /gene="yqhD"
                     /locus_tag="KKO08_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBD6317578.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20159..20986
                     /gene="dkgA"
                     /locus_tag="KKO08_001258"
     CDS             20159..20986
                     /gene="dkgA"
                     /locus_tag="KKO08_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBD6317579.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21186..22112
                     /locus_tag="KKO08_001259"
     CDS             21186..22112
                     /locus_tag="KKO08_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBD6317580.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22163..22420
                     /gene="yqhH"
                     /locus_tag="KKO08_001260"
     CDS             22163..22420
                     /gene="yqhH"
                     /locus_tag="KKO08_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBD6317581.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22463..24682)
                     /locus_tag="KKO08_001261"
     CDS             complement(22463..24682)
                     /locus_tag="KKO08_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBD6317582.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24793..26205)
                     /gene="ftsP"
                     /locus_tag="KKO08_001262"
     CDS             complement(24793..26205)
                     /gene="ftsP"
                     /locus_tag="KKO08_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBD6317583.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26280..27017)
                     /gene="plsC"
                     /locus_tag="KKO08_001263"
                     /gene_synonym="parF"
     CDS             complement(26280..27017)
                     /gene="plsC"
                     /locus_tag="KKO08_001263"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBD6317584.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27251..29509)
                     /gene="parC"
                     /locus_tag="KKO08_001264"
     CDS             complement(27251..29509)
                     /gene="parC"
                     /locus_tag="KKO08_001264"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBD6317585.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30055..30537)
                     /locus_tag="KKO08_001265"
     CDS             complement(30055..30537)
                     /locus_tag="KKO08_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBD6317586.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30590..30982)
                     /gene="ygiW"
                     /locus_tag="KKO08_001266"
     CDS             complement(30590..30982)
                     /gene="ygiW"
                     /locus_tag="KKO08_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBD6317587.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31134..31793
                     /gene="qseB"
                     /locus_tag="KKO08_001267"
     CDS             31134..31793
                     /gene="qseB"
                     /locus_tag="KKO08_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBD6317588.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31790..33064
                     /gene="qseC"
                     /locus_tag="KKO08_001268"
     CDS             31790..33064
                     /gene="qseC"
                     /locus_tag="KKO08_001268"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBD6317589.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33110..33442)
                     /locus_tag="KKO08_001269"
     CDS             complement(33110..33442)
                     /locus_tag="KKO08_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBD6317590.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            33761..34342
                     /gene="mdaB"
                     /locus_tag="KKO08_001270"
     CDS             33761..34342
                     /gene="mdaB"
                     /locus_tag="KKO08_001270"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBD6317591.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34373..34687
                     /locus_tag="KKO08_001271"
     CDS             34373..34687
                     /locus_tag="KKO08_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBD6317592.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34735..36627)
                     /gene="parE"
                     /locus_tag="KKO08_001272"
     CDS             complement(34735..36627)
                     /gene="parE"
                     /locus_tag="KKO08_001272"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBD6317593.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36656..37237)
                     /gene="yqiA"
                     /locus_tag="KKO08_001273"
     CDS             complement(36656..37237)
                     /gene="yqiA"
                     /locus_tag="KKO08_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBD6317594.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37237..38064)
                     /gene="cpdA"
                     /locus_tag="KKO08_001274"
     CDS             complement(37237..38064)
                     /gene="cpdA"
                     /locus_tag="KKO08_001274"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBD6317595.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38089..38511)
                     /locus_tag="KKO08_001275"
     CDS             complement(38089..38511)
                     /locus_tag="KKO08_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBD6317596.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38512..39141)
                     /gene="nudF"
                     /locus_tag="KKO08_001276"
     CDS             complement(38512..39141)
                     /gene="nudF"
                     /locus_tag="KKO08_001276"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBD6317597.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39346..40827
                     /gene="tolC"
                     /locus_tag="KKO08_001277"
     CDS             39346..40827
                     /gene="tolC"
                     /locus_tag="KKO08_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBD6317598.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40975..41646
                     /locus_tag="KKO08_001278"
     CDS             40975..41646
                     /locus_tag="KKO08_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBD6317599.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41652..42812
                     /locus_tag="KKO08_001279"
     CDS             41652..42812
                     /locus_tag="KKO08_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD6317600.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42850..43638)
                     /gene="ygiD"
                     /locus_tag="KKO08_001280"
     CDS             complement(42850..43638)
                     /gene="ygiD"
                     /locus_tag="KKO08_001280"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBD6317601.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43781..44554
                     /gene="zupT"
                     /locus_tag="KKO08_001281"
     CDS             43781..44554
                     /gene="zupT"
                     /locus_tag="KKO08_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBD6317602.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44612..44782)
                     /locus_tag="KKO08_001282"
     CDS             complement(44612..44782)
                     /locus_tag="KKO08_001282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317603.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(45043..45696)
                     /gene="ribB"
                     /locus_tag="KKO08_001283"
     CDS             complement(45043..45696)
                     /gene="ribB"
                     /locus_tag="KKO08_001283"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBD6317604.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45800..45948)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46070..46360
                     /gene="ubiK"
                     /locus_tag="KKO08_001284"
     CDS             46070..46360
                     /gene="ubiK"
                     /locus_tag="KKO08_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBD6317605.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11573 a        12421 c        11586 g        10955 t
ORIGIN      
        1 taaatattga atattttgat taatccccgt actaattatt cttcataatc agtacggggt
       61 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
      121 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc aacggtgaag
      181 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
      241 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
      301 aggtcgatat tgctaccaca gcgaccggcg atcggtttca ctgcgtaacc tgttttcacc
      361 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
      421 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagacc
      481 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
      541 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
      601 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
      661 ccggcagcat cccagcccag ttcatccagc ccgcgcaaga tacgcgtttc aaagcccgcc
      721 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
      781 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccag ttcgttaatc
      841 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
      901 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtattcgta aaccttcagg
      961 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     1021 caggagagac gcaaacgtgg ccagaggatt ttcggaatgt cgaacagcgc cagcaggttg
     1081 tcatctttca gcaccttgtc ggttgcgtga agatacatca gatgcagctc gttggtggct
     1141 ttaattagct cctgctcggc actctcggta atggtgtagt aatgatacgg atcctgattg
     1201 atcacctgac cgttggcctg cacataggcg ttttgtagcg gatctttttc atccagccat
     1261 ttaccgtcaa actggccttt gttttccaga cgcgctccac tgattttcag cagctcgcct
     1321 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     1381 atggtggtgt catcaaaagt gtctttaagg gtatagcagc cgttttcgac caccatctcc
     1441 agctcgcgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     1501 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     1561 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     1621 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     1681 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     1741 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     1801 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     1861 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     1921 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     1981 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     2041 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     2101 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     2161 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     2221 cgttttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacatcca ttgcaacttt
     2281 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctactggcgc
     2341 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     2401 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     2461 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     2521 aatttggcta cttcctgccg caggatctgg caaaacggac tgaaacgttg aactggctgt
     2581 tctggttaca gggtgccgcg ccgttcctcg gcggtggttt tggtcacttt taccattacg
     2641 cgccggtgaa aattgagtac gccatcaacc gctttaccat ggaagccaag cgcctgctcg
     2701 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     2761 cggatatggc gatttggccg tggtttggca acgtggtgct cggcggcgtg tatgatgccg
     2821 cagagtttct ggatgcggga agttataaac atgtgcaacg ctgggcgaaa gaagtgggcg
     2881 aacgtccggc agtgaaacgt gggcgtattg ttaaccgcac caacgggccg ctgaacgagc
     2941 agttgcatga gcgccatgac gccagtgatt ttgagacaaa tacagaagat aagcgtcagg
     3001 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     3061 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacaaaag
     3121 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     3181 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     3241 ccagcagatc ggcagggtta ccttcgcaaa tcagggcgat attcacgtcg cgcttgatac
     3301 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     3361 gaacgccaat acacattgtt aactccggtt atttcgattg ccggacaaac gcgaaaaaga
     3421 acctttagcg ggtgttccct ctcaccctaa ccctctcccc ggaggggcga ggggaccgtc
     3481 cgtgcgcttt ttaggcgttg tcctcagtct tattcaactt caatactttt gacgatcagc
     3541 gaatcgccgg tatcgacacg caaccgctcg ccgtgacaga gcggacactg cgcatcgtgc
     3601 tgatgaatct ccaccacctg gctgcaatcc cagcaccagg cctgggcggg tttatagacg
     3661 atatgtaaat cgcacccttg cgccaccgtt ccgtggcaga caatttcaaa actaaaacgg
     3721 acggcgctct cctcaacgca ggagagcgcg ccaatttcca gccacacggc ggtgacgcgc
     3781 ttaacatcgt gctgctccgc ctgccgttgg ataatttcaa cggcgctctg gcaaagcgac
     3841 aactcatgca ttttcgctcc tcctgcggcc aaacagcagg gcgcgacgcc ctgcgtgtgg
     3901 tacatccgga ttggtgactg gcaacgaaag gatcattcgt gcgcagtcat ccgtcaggcg
     3961 ttggccttct tcaatcgaca tgctgtgcga aagcggcgac atcagcgaac aggagaggta
     4021 ttgcgaaaca ccgtccagtt cgccaacggt aaaggtcatg gtgccatacg gcagttgcag
     4081 accaattttt tcactcactt tgcgcagcgg ccagagttga tccgggccgg ggaagataac
     4141 tgcactcagc atccacgggg tgatcacaca ccccgtccac tgaccttcga acagcgtaaa
     4201 atcagaaaca tacaccggca ttgaaggatg cagaaaagag agatcgtgca tcgaacgccg
     4261 ggcaatttct tcaaacgctg cctgtacttg cgccttcggg gaggtctgga aacctgctat
     4321 ctcttcagtc atgaatcgcc tcccgtggga tggcttccac gcctgattcg cgcagcgcag
     4381 ccagaacctg ctcaagcgca ggttcaatca ttgcttcaac cgtcggcgtt aagccgatgt
     4441 gtggctccag cgattccggg atcacgccga ccagggtcag ctttttcgga aactcaccgg
     4501 tgaagcgcag ggccgacaat acgtcggcca ggccaagctg atgcggagag attttgttgg
     4561 taaacaacgc cggaacttct tcatcccgca ggatcatcat cgttcccgga gtgttctttt
     4621 tcgacacaat ggcatccgca ataatcagat gatcgcgatt tgccatgtcg ccaagcagct
     4681 ccattcccgc cgtgccgcca tcgaggatct caacataatc cggcagaatg tatcgttgct
     4741 ctaacgcttc gacaatccgc acgccgatgg cttcatcggt cagcaaaata ttgccgaccc
     4801 ctaagactaa aatacgcatt acagaacctt cactgaaacc acttcgttgc cgtcggcatc
     4861 cactacgtgt accgcacagg ccatgcacgg gtcgaaggag tgaatggtac gcaccacttc
     4921 cagcggttta ttcggatcgg caaccggtgt acccaccagc gactgctcgt aaggaccgac
     4981 gtcatcattg aagttacgcg gaccagagtt ccaggttgat ggaacaaccg cctggtagtt
     5041 gctgatgata ccgtctttaa taaccatcca gtgagagagc ataccgcgcg gcgcttcgag
     5101 gaagccaaca cctttgaact cacccgttgc cggaatgttc ggcttcacaa aggtggtgtg
     5161 atcgcctttg ccgatattgg tgatcagtgc actgtattgg ttttgcagga tatcctgcaa
     5221 ttcacagcag tgaacggtac gaccaataat acggcccagc gtggagtgca gctgtgccac
     5281 ttccagcgtg ttgccagtca gtttctgata aatcgcaacg atttcattca gtttgttctg
     5341 ggtagattcg cgacctgccg ccagtttcac cagcatatta gccagcggac ccacttctac
     5401 cgttttgccg tagaaagtcg gtgatttcac ccaggaatat ttcccgtcgt cagaccaacc
     5461 atcataagcc ggaatggtgg taccttccca cggcgcctgt ggcgcttcgt ctttatacca
     5521 ggagtgcttc gcgctttcct gaatgccttt gatcaggtat tcatcggaat gagaagtgat
     5581 cggacgatac gaggacagat ccgcattctc aatgtagcct cccgggaaca ggaagctgcc
     5641 gtttttgctg tcggtcggga attccggcac gctcaggtag ttcaccgcac ctttaccgcg
     5701 cgtcagccat tccgggtaga acgcggcgat aaccgcggta tcaaccttat aaacctgctc
     5761 aacaaagtcg ctcagtttgt cgatgaaaga cttgatgtac atcaggcgct caaggttcaa
     5821 cacgcccaac ccgtcgaggt tgattgggtt cgcgacacca cccactgcca ggttctgaat
     5881 gtgcggcgtt ttaccgccca gcagcgccac gacgcggtta gcgtcacgct ggcactccag
     5941 cgcctgcagg tagtgcgcta ccgcaatcag gttcacttcc ggcggcagtt tcatcgccgg
     6001 gtgaccccag tagccattgg cgaaaatacc caactgaccg ctggcaacca gatctttgat
     6061 cttgttctga actttagtga actcttccgg actgttcaga tgccaggtcg aaacgccttt
     6121 cagcatttcg gaggctttgg ttgggtcagc ttgcagtgca gaagtgatgt ccacccagtc
     6181 cagcgccgaa agctgataga aatgaacgat atggtcatgc gtggtgtgcg cagccagaat
     6241 gatgttacgg atgtattgcg cgttaaccgg aacgtcgata ttcagcgcac tttctgccgc
     6301 acgaacggaa gacagcgcgt gagtggtagt acatacgcca cagatacgtt gcacaatcat
     6361 ccatgcatcg cgcggatcgc ggtttttcac gatctcttcc atgccgcgcc acatggtacc
     6421 ggaagcccat gctttcgaaa cgacgccatt ttcgatttcg caatcgatgc gtaaatgccc
     6481 ctcaatacgg gttaccggat caatagtaat tctctggctc atgctttgct cgcctcatga
     6541 cgattatgat cgttttgttt taaaggagga agtatcggca gtagacgaat gagtacgatg
     6601 taagcgcaaa tctcaatagc cacaaaacca atagaaatca acagttcttc ccaggtcggg
     6661 aagtaggcgt aaccgccgcc cgggttgaat gccaccaacg aataggtcag acgccaggtt
     6721 gcacaaccta acagtgcgct cagtgctgac aggaacagca tgcgggaatc attacgcagc
     6781 ttcgccacac gcagaacgac cagcgggaag agcatcagca ggacttcaat ccagaacatc
     6841 acggagtaga agtcaccggc aaacgctaac gacagcttgt cgcgatagat aagctcgcca
     6901 aagcgcagcc cgatgaaaat cgccagcaac acactgatgg tgttggtcag cttgacgaac
     6961 aggctctttt catccggacc gttgccacgc agacccgcct gcaccagtga accttcaaag
     7021 atgacaatcg agaagcccat gatgaacgcc gtcagcagcg agaacagcgg caacatttca
     7081 tagctctgcc agagcggatg caccttgtag cccgccgaga tcatcagcga ccccattgaa
     7141 gactggtgca tggtcggcag cagcgcaccg agcgcgatga tgaagaacat caccttgttc
     7201 agacgttgta gcgacacctt ccagcccaga cgttcaaaca gtgccggagc aaactccaga
     7261 gccatcacgc cgatgtagat ggtcatacag accgccgtct cgaacagtac cgagttcacg
     7321 ttgaagtgac ccggaatgta gaagtacggc aggttccagt agcgacccac gtcgatagtg
     7381 atcgacaagc cacccagtga gtaaccaaac agactcgcca acagcgccgg acgcaccagc
     7441 ggatggtatt gcccacggtt aaagacgtat accgcccacg ccagcgccca gccgccacag
     7501 gcaaagccgg tgccaatcag caggtcaaac gcgatccaca cgccccacgg gaagccgccg
     7561 ttcaggtcag agacagagcc cagaccgaac accagacgct tcacaatcag gagcatacag
     7621 atgacgatta acggtccaaa aatcatgacc ggtttactga tgattttgcc gcccagcggt
     7681 tgtggatcat gactcatgat cgtctcctcc gtcgtgatgg tcgtttttgg tgttgcgacg
     7741 aaccagcacg gttaagcccg ccagcacagc cagtggtagc atcatgcctt tatacagggt
     7801 gtgttgaata ttttcggaac gcgcaccggt agaaagatcg tccagtttcg gcaggtcgag
     7861 attttcataa ggcacacccg tcagtaccag aacctgagta ccgccgccct ctttctcgcc
     7921 gtacagatgc ggataatatt tcggcaccgt atgcaggtaa gtgtcgccag atttcagcgt
     7981 ctgacgcgga tagtggtatt cgctgccagg cttcagcgcc agacgttttt tcgcctccgc
     8041 catcagctct tcacgcgtac cgaaaatcac cgcgcccgcc gggcacactt ctacgcagcc
     8101 aggcagaccg cctttatcga gacgttccac acctttctgg ttgcacagct cgcacttatg
     8161 cagcgcacca aacgggttgt tgtagtcgta cttcggcacg ttgtacggac aggcgaccat
     8221 gcagtaacgg caaccggtgc acacgtcttt gtcgtaatgg acaatgccgg ttttcggatc
     8281 ttttttcagt gcagagaccg ggcacacaga gacacagttc ggatcgacgc agtgcataca
     8341 ctgtttctta atgtacgcgt agccgttctc ctcctggtct ttgttgaccc ctgtgccgct
     8401 ggtccacacc tgaatgatgt tattggtata cggcgacagt ttgtcgttgt tcgaccaggt
     8461 ctgttcccct tgcgggttac gttcagggaa gttgatatcc tgacacttgg tgacgcaagc
     8521 ctggcagcct acgcacaagg tcgagtcgta caacatcccc agcgatcccg gaattggcgg
     8581 gcggttttca gcagccgcat gactgacaga cggcagcgcg cccgtcagca atgccccgca
     8641 ggaggctgct ttaataaaat tacgtctgtt cacggttatt ctccccgtga gtcagcgtta
     8701 tctttctttt gctgacgacc cagttcacgc accgccatca cgctgacacc ggcaaccagc
     8761 ccaaccacac cgccgagcaa accaatagcg cctgcagaga cgttgccgcc ctctttagcg
     8821 ttaacatccg gtttctgtga acgcggagtc tgattttcga cgttggcaag ctgatggatg
     8881 cctttatgga agccgatacc tttttcgtta cagccatagc acgggtgacc aatcgccacc
     8941 ggccacacac cgccaacatc gcagaattgc agcgttgagc agttgccgta agtttctggc
     9001 cctttacagc cgaggtggta caggcaccag ccttcgcggt ggccttcatc accgaactct
     9061 ttggcaaaac gaccagcatc gaagtgcggg cgacgttcgc agtgttcgtg aatcagacgg
     9121 ccataggcga aggtcggacg gtttttgtca tccagtttcg gcggtttgcc gtaagtgatg
     9181 atgtgcgcaa cggtcgcgag gaagttgtgc gggttcggcg ggcagcccgg aatattgata
     9241 acggttttgc ctggcagaac ttcttgcaag ctgactgcgc cagttgggtt aactccagct
     9301 gcggcaacac cgccccacgc ggagcaggaa ccaatagcga taatggctgc tgagccttcc
     9361 gccgctttgc ggatgtgatc cacaatcggc tcaccggcaa ccatgcaata aataccgtta
     9421 tcttttaatg ggatggaacc atccaccact aacacatact gccctttgta cttctcgaga
     9481 gcgttatgtt tgttctcttc gacctgatga ccgaaggcgg cggaaagcac ttcgtgatat
     9541 tccagagaga tagtctccag cacgaggttt tctaccgttg gatgcgttgc acgaagcaga
     9601 gattccgtac aaccggtgca ctcctgcgcg ccaatccaga taactggcgg acgctgcggg
     9661 ttagtaaccg attcggccat ctctgcagcg gctttgctac ttaaccccat ggtggcggct
     9721 aatgctgcac aaagcttcat gaaatcacga cggttaatgc cgtgagaatg gatgagggtg
     9781 ttatctccag tcatttatag ttattccgtt gcgaggacct ggcatatatt ttgcctcaat
     9841 cgcaaaatca ataatgcgat cgatgcgcca tttaccacac atttattatg gttatcgata
     9901 tcatgatact gcggcggggc gctaaaacga agggaagaag cattagtgta attaattaaa
     9961 gcaagataat acgtatgttt gatcaatttt cgaggcaata accggccata aaaaagcgca
    10021 acgtagaaca ggaattattc tcacgaaaga aatccattac catctcttta tccagtccat
    10081 agcggcgcgt cagaataccc gcttcccagg cgctaagctg gcgatctccg gtcttttcgt
    10141 acatccggtg tgagtagcct aatacaaaac cgcgtttata atcggcacag aattttgtca
    10201 cattaaccgc actgtcggca tgcgttgctt ttaatcccgc cattaagcct ttaccaaaat
    10261 ggttattcat cgttcacctc attcgttata tatcgccata tatagcgata ttttaggcaa
    10321 agaacgactt ttgcgcacgc tattttttca tacttgttta agacgtaatc ccgtaagaga
    10381 taaacctatc aggagagata tttatcgaac catttcagtg tcctttgcca ggcaagatcg
    10441 gcggcagatt tgtcataacg gggcgtggaa tcattatgga atccgtgatt aacccccgga
    10501 tagatataag cctcataaac cttattattg tctttcaacg ccgcctcgta aggaggccag
    10561 ccctcgttga ttcgggtgtc cagttccgcg aagtggagta gtaaaggcgc ttcaatcttc
    10621 gccacatcgg cagtgggtgc ctgacgacca taaaacggca ccgcgcaggc cagttccgga
    10681 tacgcgacag ccgccgcgtt cgatacgcca ccgccatagc aaaatccggt aatacccact
    10741 ttacctgtcg cttgcggata gcgttgcata aactcaattg cggcaaagaa atcattcatc
    10801 agtttggttg gatcaacctg ctgttgcagc tcacgacctt tatcatcatt tcccggataa
    10861 cctccaacgg aacttaatcc gtcaggtgcc agggcgatat accccgcctt cgccactcgc
    10921 cgtgccacat cttcgatata cggattcagt ccacgattct catgcaccac ccccacggct
    10981 ggcgttttgc cgctcatttt tgcgggtttc accagataac cccgtacctc gccgtgacca
    11041 tttggcgaag gatacgtgat gtactcagca acaatctccg ggtcggtaaa ctctacctga
    11101 gtcgccagcg cataatttgg cttgagcaaa tcaaacaacg ctaatgccgt catcccgccc
    11161 accgcatact tcgccgcaag gttgaggaac tcacgtttcg aaattttccc gtgaacgtaa
    11221 tagtcgtagt aatccaacaa ctcttgtggg aaatctttgg cggttaaacg cggcatcgtt
    11281 gcattcctca actggtgttt ttttaagcaa agcataagcg cgtgtttttg cccagttttt
    11341 cgtcactctg tgagccagac tacgggatac gcgctggcga atcgctaaac tagaaacatt
    11401 gtttcgaaat tgaacggagg aaaggagagg tcatggtctg gttagcgaat cccgaacgtt
    11461 acgggcagat gcagtaccgc tattgcggaa aaagtggttt acgcctgccc gcattatcgc
    11521 tcggtttatg gcacaatttc ggtcacgtta acgcgctgga atcacagcgt gcaatcctgc
    11581 gtaaagcgtt tgatttaggc attacgcact ttgatttagc caacaattac gggccgcctc
    11641 caggaagcgc agaagagaac tttggtcgcc tgctgcgcga ggattttgct gcttatcgcg
    11701 atgaactgat tatctctacc aaggctggct acgatatgtg gcccggcccg tacggctctg
    11761 gcggttcacg taaatacctg ctcgccagcc tcgaccaaag cctgaagcgt atggggcttg
    11821 agtatgtcga tatcttttac tctcatcgcg tcgatgaaaa tacgccgatg gaagaaaccg
    11881 cctctgcgct ggctcatgcg gtacaaagcg gtaaggcgct gtatgtcggg atctcctctt
    11941 actcgccaga gcggacgcaa aaaatggtcg agttgctgca cgagtggaaa attccgcttt
    12001 taattcatca accttcgtac aatttactga accgctgggt ggataaaagc ggcctgctgg
    12061 ataccctgca aaataacggc gtgggctgta ttgcctttac tcctctggct cagggattgc
    12121 tgaccggaaa atatctcaac ggcattccgc aagattcacg gatgcatcgt gaagggaata
    12181 aagttcgtgg tctgacgccg aaaatgctta ccgaagccaa cctcaacagc ctgcgcttat
    12241 tgaatgaaat ggcacagcag cgtggacaat caatggcgca aatggcgtta agctggttgc
    12301 tgaaagatga tcgcgtgacg tcggtattga ttggtgccag ccgcgcggag caactagagg
    12361 agaacgtgca ggcgctgaat aatctgacat ttagcaccga ggagctggcg cagattgatc
    12421 agcatatcgc cgatggcgag ctgaatctgt ggcaggcgtc gtccgataaa tgacctgtta
    12481 ataacgggcg cgcgaaccgc gcccgaatca gatcagtgat tatgacgagt cagtcggtca
    12541 agatagccca tcacaaatgc agaaagcaca aacgtcagat ggataatgac gtaccacatc
    12601 agtttgttat ccgggacatt tttcgcatcc ataaagacgc gcagtaagtg aatggaagaa
    12661 attgccacaa tcgacgctgc tactttgttt ttcagcgacg ttgcgtccat tttccccagc
    12721 cacttcagct tctctttgtt ctcggagata tccagctgtg agacgaaatt ctcataaccg
    12781 gaaaacatca ccatcaccag caaaccgcca accagcgtca tatccaccag cgacagcaac
    12841 acgaggatca aatctgattc cgccatcgag aagatattcg gcagtacgtg aataatctcc
    12901 tggaagaact tcagcgccag ggcaactaac gccagcgaaa ggccaaagta cacgggggca
    12961 agcagccagc gagaagcata cattgcattt tcaagaaaac gttccataca ttcctgtcgt
    13021 gtagttaaaa cgggcaacag tatatcgcaa ttgagtaaac agcacgcaac agcaaatcaa
    13081 catatacgtt aaacctttgt cgcataattc tgaggtttag ggatcttcct ggttccctta
    13141 tcaacgcatc gagagcgtct acccttaaag gacgttgaac ctgaagggag aaaaacgatg
    13201 tctcatttaa aagacccgac cacgcagtat tacactggtg aatatcccaa acagaaacaa
    13261 ccgacgccag gcatccaggc gaagatgaca ccgataccgg attgcggcga gaaaacctat
    13321 gttggtagcg gtcgcctgaa agatcgtaaa gcactggtga cagggggcga ttccggaata
    13381 ggtcgcgctg ccgccatcgc ttacgcgcgt gaaggggctg acgtggcgat cagttatctt
    13441 cccgtggaag aagaagacgc tcaggatgtg aaaaagatca ttgaagaatg cggacgcaaa
    13501 gccgttctgc tgccaggcga tttaagcgat gagaaattcg cccgttcgct ggttcacgaa
    13561 gcgcacaagg cgttaggcgg gctggatatt atggcgctgg tcgccgggaa acaggttgcc
    13621 attccggata ttgcagacct caccagcgaa cagtttcaaa agacctttgc cattaacgtt
    13681 ttcgcgctgt tctggctaac ccaggaagcg atccccctgc taccgaaagg tgcaagtgtc
    13741 atcaccactt cgtcaatcca ggcataccag ccaagtccgc atttactgga ctatgcggct
    13801 acgaaggcgg cgattctgaa ctacagccgt ggcctggcaa aacaggtcgc ggagaaaggt
    13861 attcgggtga atattgtcgc gccaggccct atctggacgg cactgcaaat ttccggcgga
    13921 caaacgcagg ataagatccc gcagtttggt cagaaaacgc cgatgaagcg tgcggggcaa
    13981 ccggcggaac tggcccctgt atatgtttat ctggcaagtc aggagtcgag ctacgtcacc
    14041 gcagaagtgc acggcgtgtg cggcggcgag catttaggtt aaaaaaatgc ccggttgtga
    14101 aaagcaaccg ggcatcattg tgaattcact cttacctgat gtggcgaaaa cgccttatcc
    14161 gcctacaaac gcatgaagat tcaacatgtt gcaatagatt ttgtaggcct gataagcgta
    14221 gcgcatcagg cattgagcac ctaatgcctg atgcgtcgtg aacgccttat ccggcctaca
    14281 aacgcacgaa gattcaacat gttgcaatag attttgtagg cctgatgagc gtagcgcatc
    14341 aggcaattct actttacttc gctttggcgg tttcttcgcc gaccagacct atcttcaggt
    14401 aacccgcctg atgcagcgta tccattacct tcatcaacgt ctcgtaatcg acggttttgt
    14461 ccgctcggaa gaagatggtg gtgtctttct tgccttcggt taacgcattc aacgccgtaa
    14521 tcattgtttc atcggtgacc ggatcgttac cgataaacat cgagttgtct gccttcaccg
    14581 acagataaac cggtttttcc gggcgcggct gcggcgtgct ggtagaagca ggcaagttca
    14641 ccttcacatc taccgtcgct aacggtgccg ccaccataaa gataatcagc agaaccaaca
    14701 tcacgtcgat aaacggcgtc acgttgatat catgcatttc gccgttatcg tcgaggtttt
    14761 cgttaagatg cattgccatc ggacattatc ctgcgcgtaa tttttgtgcg acacgaaccg
    14821 gatgcgcagc ggcgctggct tccagatcca ggtcacggct ttgcagcaac aatacctgcg
    14881 ctgcaacatc acccagcatc gctttaaagc cgccaatctg gcgtgcaaat acgttataga
    14941 taacgaccgc aggaatcgct gccacgaggc cgattgccgt tgctaacaga gcttctgcga
    15001 tacccggcgc aacgactgcc aggttagtgg tctgcgtttg cgcgatacca ataaagctgt
    15061 tcatgatgcc ccagaccgta ccaaacagac caacgaacgg agaaatcgcg ccgatggttg
    15121 ccagatagcc gttaccgcgg cccatttgac gacccactgc ggcgacccga cgctccagac
    15181 ggaagctggt acgttcttta ataccttcgt tatcgtcgct gccttctgac agttccagct
    15241 cattctgcgc ttcattgagc aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga
    15301 tatcgttggc ctggtttaag gaacgtgctt cagccagcag ttgctgctcg cgcttaaggc
    15361 gacgcttctg attgaagaac tctacgctct tactgaagaa gattgcccag gtgactacag
    15421 aggccaaaat aagcccaatc atcacgcact taacgacaat atcggcgtac tgatacatac
    15481 cccagacgga aaggtccgtc tgcattaaat tattacccac tgtgtatctc caggacgcaa
    15541 gtcacaaaat ctgcgcataa taatatcaaa acgacgtcga attgatagtc gttctcatta
    15601 ctatttgcat actgccgtac ctttgctttc ttttccttgc gtttacgcag taaaaaagtc
    15661 accagcacgc catttgcgaa aattttctgc tttatgccaa ttcttcagga tgcgcccgcg
    15721 aatattcatg ctagtttaga catccagacg tataaaaaca ggaatcccga catggcggac
    15781 aaaaagcttg atactcaact ggtgaatgca ggacgcagca aaaaatacac tcttggcgcg
    15841 gtaaatagcg tgattcagcg cgcttcttcg ctggtatttg agagtatgga agccaaaaag
    15901 cacgcgacgc gcaatcgcgc caatggcgag ttgttctatg gacggcgcgg aacgttaacc
    15961 catttttcct tacaacaagc gatgtgtgaa ctggaaggtg gcgcaggctg cgcgctattt
    16021 ccctgcgggg cggcagcggt tgctaattcg attcttgctt ttgtcgaaca gggcgatcat
    16081 gtgttgatga ccaacaccgc ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc
    16141 aaactgggcg taacgacatc gtggtttgat ccgctgattg gtgccgatat cgttaagcat
    16201 ctgcagccaa acactaaaat cgtgtttctg gaatcgccgg gttccatcac tatggaagtc
    16261 cacgacgttc cggcgattgt tgccgccgtg cgcagtgtgg tgccggatgc catcattatg
    16321 atcgacaaca cctgggcagc cggtgtgctg tttaaggcgc tggattttgg catcgatgtt
    16381 tctattcagg ccgccaccaa atatctggtt gggcattcag atgcgatgat tggcactgcc
    16441 gtgtgcaatg cccgttgctg ggagcagcta cgggaaaatg cctatctgat gggccagatg
    16501 gtcgatgccg ataccgccta tataaccagc cgtggcctgc gcacattagg tgtgcgtttg
    16561 cgtcaacatc atgaaagcag tctgaaagtg gccgaatggc tggcagaaca tccgcaagtt
    16621 gcgcgagtta accaccctgc cctgcctggc agtaaaggac acgaattctg gaaacgagac
    16681 tttacaggca gcagcgggct attttccttt gtgcttaaga aaaaactcaa tgatgaagag
    16741 ctggcgaact atctggataa cttcagttta ttcagcatgg cctactcgtg gggcgggtat
    16801 gaatcgttga tcctggcaaa tcaaccagaa catatcgccg ccattcgccc acaaggcgag
    16861 atcgatttta gcgggacctt gattcgcctg catattggtc tggaagatgt cgacgatctg
    16921 attgccgatc tggacgccgg ttttgcacgg attgtataac attgccactt ttggacaatt
    16981 ttgcagacat tttattgtga aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg
    17041 acgattcagg agtacaatag gcagataaag gcttaaacgc tgttccacag gaaagtccat
    17101 ggctgttatt caagatatca tcgctgcgct ctggcaacac gactttgccg cgctggcgga
    17161 tcctcatatt gttagcgttg tttactttgt catgtttgcc acgctgtttt tagaaaacgg
    17221 cctgctgccc gcctcatttt tgccaggcga cagcttgttg atactggcag gcgcattgat
    17281 tgcccaaggg gttatggatt ttctgcctac gattgcgatt ctgaccgccg cagcaagtct
    17341 gggctgctgg ctaagttata ttcaggggag ctggttaggg aataccaaaa cggtgaaagg
    17401 ctggctggca cagcttcctg ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca
    17461 cggtctgctg gcgctgctgg ctggacgttt tcttgcattt gtccgtacgc tgctgccaac
    17521 catggcggga atttccggtc tgccaaaccg ccgcttccag tttttcaact ggttaagtgg
    17581 attgctgtgg gtcagcgtgg taaccagttt tggctatgcc ttaagtatga ttccgttcgt
    17641 taaacgccat gaagatcagg taatgacgtt cctgatgatc ctgccaattg ccttgttaac
    17701 cgctggcttg ttaggcacgc tgtttgtggt gattaaaaaa aaatactgta acgcctgacg
    17761 attttccccg ttcccggctg ctgtaccggg aacgtattta attcccctgc atcgcccgca
    17821 ttcttgccgc atcttccccc ggcgtcacac cgaagtaacg tttaaactca cggctaaatt
    17881 gcgatgcgct ttcatagccg acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga
    17941 tgatcatcat ccgcgcctta tgcagacggt aattcttcaa atactgcaac ggcgaggtgc
    18001 tggtgacaga cttaaaatta tggtggaacg ccgatacgct catgttggct tctgccgcca
    18061 gttgctcgac gctcaggttt tcggtgtatt tattctcaat tcgtttcagc acgcggctaa
    18121 tcagactgaa gtgagtctgg cgactgacca gcgccagtaa cgcgccgccg caaggtccgg
    18181 tcagcacgta gtacagaatt tcgcggatga tctgtttgcc gagaatacgc gcatccagtg
    18241 gtcgctccat cacgtcgagt aaccgctccg ccgcgcataa aatctcttct gataacgtgg
    18301 cggagttaat cccgctggct gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca
    18361 tcaacagttc ctgtaactgc aaaatatcga cattgagacg caaccctgcc agcggcacct
    18421 ctgacgtggc ataggtttcg cactcaaacg gcaacggcac cgtcagcagc aggtattcat
    18481 tggcatcata acgaaacacg cgttcattga tataaccgat tttatgcccg gaaaagagaa
    18541 ttatgatgcc aggctcgtac atcaccggtg tacgtgcgaa aggcgtctcg ccatacaaca
    18601 aacgcacatc gggcaacagt tctgacaaac tattttcttt atttttcagt ttattaactt
    18661 tatccgccag caagcgacaa atctcttcac gtttcatatc gcgtaatttc ttaggaataa
    18721 tgcggcaatt tgattgtgcg caattttgta gcatttctcc agcactctgg agaaataggc
    18781 aagacattgg cagaaatgag cattgagagc cagggcgctg gcgatcacaa tgaaaaacat
    18841 caggcagatc gttctctgcc ctcatattgg cccagcaaag ggagcaagta atgaacaact
    18901 ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct ggtttacgcg
    18961 aacaaattcc tcacgatgct cgcgtattga ttacctacgg tggcggcagc gtgaaaaaaa
    19021 ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg gaatttggcg
    19081 gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg gttcgcgaac
    19141 agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc accaaattta
    19201 tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg caaacgggcg
    19261 gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca gcaaccggtt
    19321 cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag caggcgttcc
    19381 attctgcaca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc tacaccctgc
    19441 cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg gaacagtatg
    19501 ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt ttgctgacgc
    19561 taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg cgcgccaacg
    19621 tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cactggcgta ccgcaggact
    19681 gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat cacgcgcaaa
    19741 cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgagaccaag cgcgctaagc
    19801 tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgacgat gagcgtattg
    19861 acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg acccacctct
    19921 ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg gaagagcacg
    19981 gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc cgtatatacg
    20041 aagccgcccg ctaagctttt tacgcctcaa actttcgttt tcgggcattt cgtccagact
    20101 taagttcaca acacctcacc ggagcctgct ccggtgagtt catataaagg aggaacgtat
    20161 ggctaatcca accgttatta agctacagga tggcaatgtc atgccccagc tgggactggg
    20221 cgtctggcaa gcaagtaatg aggaagtaat caccgccatt caaaaagcgt tagaagtggg
    20281 ttatcgctcg attgataccg ccgcggccta caagaacgaa gaaggtgtcg gcaaagccct
    20341 gaaaaatgcc tcagtcaaca gagaagaact gttcatcacc actaagctgt ggaacgacga
    20401 ccacaagcgc ccccgcgaag ccctgctcga cagcctgaaa aaactccagc ttgattatat
    20461 cgacctctac ttaatgcact ggcccgttcc cgctatcgac cattatgtcg aagcatggaa
    20521 aggcatgatc gaattgcaaa aagagggatt aatcaaaagc atcggcgtgt gcaacttcca
    20581 gatccatcac ctgcaacgcc tgattgatga aactggcgtg acgcctgtga taaaccagat
    20641 cgaacttcat ccgctgatgc aacaacgcca gctacacgcc tggaacgcga cacacaaaat
    20701 ccagaccgaa tcctggagcc cattagcgca aggagggaaa ggcgttttcg atcagaaagt
    20761 cattcgcgat ctggcagata aatacggcaa aaccccggcg cagattgtta tccgctggca
    20821 tctggatagc ggcctggtgg tgatcccgaa atcggtcaca ccttcacgta ttgccgaaaa
    20881 ctttgatgtc tgggatttcc gtctcgacaa agacgaactc ggcaaaattg caaaactcga
    20941 tcagggcaag cgtctcggtc ccgatcctga ccagttcggc ggctaacgtg caaattctcc
    21001 cggtggcggt aatgttccgc taccggactt ttcagaaatc atttattccc ctcgcgtccc
    21061 gcccgttgtt actcttcctt gttcaggaat gccaaatata aggacatcat catgcagagc
    21121 cggaagctct taaaagaaca actcatctat atccgggata aacgcaacgg agaggtgaaa
    21181 aacagatgaa aataatactt ctgtttttag cagccctggc aagttttacc gtacacgcgc
    21241 agcctccttc acagaccgta gaacaaacag tccggcagat ttatcagaac tataaatcag
    21301 atgccagtac gccttatttc ggtgaaaccg gagagcgggc gataacttca gcgcgtattc
    21361 aacaggcgct taccctgaac gacaatctta cactaccggg caatattggc tggctggatt
    21421 atgatccggt ttgtgattgt caggattttg gcgatctggt gttagaaagc gtagcgataa
    21481 cccaaactga cgtcgatcat gccgatgccg ttgtgcgctt tcgtatcttt aaagatgata
    21541 aagaaaagac cacacagaca ctgaaaatgg tggcggaaaa tggtcgttgg gtcattgatg
    21601 atattgtcag caatcatggc agcgtcttac aagcagttaa tagcgagaat gaaaaaacgc
    21661 tggccgcttt agcttcattg cagaaagaac agccagaatc ctttgttgct gaactctttg
    21721 aacatattgc tgattatagc tggccgtgga cgtgggtggt ttccgactct taccgccagg
    21781 cggttaatgc cttctataaa accaccttca agacggccaa taatcccgat gaagatatgc
    21841 aaatagaacg gcaatttatt tacgacaatc cgatctgttt tggcgaagag tcgctatttt
    21901 cacgcgttga tgaaattcga gtcctggaga aaaccgccga ttccgcccgc attcatgttc
    21961 gttttacgct gaccaatggc aacaatgaag agcaagagct ggttttacag cggcgcgaag
    22021 gtaagtggga aatcgctgat tttatccgcc cgaacagcgg cagcctactt aagcagattg
    22081 aggcaaaaac cgccgccaga ttaaagcaat gagctgaatt aaataacaat tagccggaac
    22141 aataaataaa agggaacact atatgaaaac gattttcacc gtgggagctg ttgttctggc
    22201 aacctgcttg ctcagtggct gcgtcaatga gcaaaaggtc aatcaactgg cgagcaatgt
    22261 acaaacatta aatgccaaaa tcgcccggct tgagcaggat atgaaagcac tacgcccaca
    22321 aatctatgct gccaaatccg aagctaacag agccaatacg cgtcttgatg cacaggacta
    22381 ttttgattgc ctgcgctgct tgcgtatgta cgcagaatga taaaaaaatc cccggcagca
    22441 tgtcagttgc cggggatttt ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac
    22501 cagcattcac cggacgagat tgcgtagacg acgctttttt tgccgtagca ggcgtctgac
    22561 gctgagtcgc catcggcgta tgtttagtca acgccggacg ggtattgcgg ttctggcgac
    22621 gagcttcacg catctcttca atggttggcg caggcactaa gcaatcgcga cggctgccaa
    22681 tcagatgctt tttgcccatc gcttccagcg cctgacggat taacggccag tttgccggat
    22741 cgtggtaacg caacaacgct ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga
    22801 cgccttcgct cttataacca atcttcgcca gcggtttttt cccggtgtaa tacatggtgg
    22861 ttgagttcgc cagcggcgat gggtagaagt tctgtacctg gtcgagacgg aaacgatgct
    22921 ttttcagcca cagcgccaaa ttcaccatat cttcatcacg cgtaccgggg tgcgcagaga
    22981 tgaaatacgg gatcagatac tgctctttac ctgcctgttt cgagtaggta tcgaacagct
    23041 ctttaaagcg gtcatagctg cccatgcccg gcttcatcat cttcgacaac ggcccttctt
    23101 cggtatgttc cggagcaatc ttcagataac cgccgacgtg atgggtcgcc aactctttga
    23161 tatagcgcgg atcttccacg gcgatgtcat aacgtacgcc tgaggcgata aggatttttt
    23221 taatgccttt cagatcacgc gcacggcgat agaggttgat cgtcggttcg tggttagtgt
    23281 ccatgtgcgg acaaatatcc ggataaacgc acgacaaacg gcgacaagtt tgttcagcgc
    23341 gtggcgattt gcagcgcaac atatacatgt tggcagttgg cccaccgaga tcggaaatca
    23401 cgcccgtaaa acctggaacg gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt
    23461 ccgaacggct ctgaataatg cgcccttcgt gctcggtaat agaacagaaa gaacagccgc
    23521 caaagcagcc gcgcataatg ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg
    23581 cattgccata ggccggatgt ggcacgcgct tgtacggcag cgcaaaaacg ctgtccatct
    23641 cttcggtaga aagcgggata gcaggcgggt tgatccacac atagcggtca ccgtgttttt
    23701 gcatcaatgc gcgggcgcag cctgggttgg tttcgtggtg cagaatgcgc gaagcatggg
    23761 cgtacagcac tttatcgccc ttcactttct cgaaagaagg cagcaatacg taggtttttt
    23821 cccacggttt ctggcgcggt ggctgcacga ttacagcttt cgcttcctgc tttttcggcg
    23881 caacaggttt gttatccgca cacggcaaat cttcgccata cggatgcggg attggggcga
    23941 ttttccctgg ggtatcaaga cgggtggaat ccacaccgct ccagccaggc agcgcctctt
    24001 ttacgataat tgcggtatta cgcacatcgc ggatttcact aatcggctcg cccatcgcca
    24061 gacgatgcac tacttccacc agcggacgtt caccgttacc aaacatcagc atgtcggctt
    24121 tcgaatctac cagaacggaa cggcgcacgg tatcggacca gtaatcataa tgcgcggtac
    24181 ggcgcaggct agcctcaata ccgccgagga tcaccggcac atctttccac gcctctttac
    24241 aacgctgggt ataaaccagc gtggcgcgat ccggtcgctt acccgcgaca ttatctggcg
    24301 tgtaggcatc atcatgacgt aaacggcgat cggcggtata gcggttgatc atcgaatcca
    24361 tgttgccagc agtaacacca aaaaacagat tcggtttacc cagacgcata aagtcgtctt
    24421 tgctgctcca gtccggctgg gcgatgatcc cgacgcgaaa gccctgcgct tccagcatac
    24481 gaccgcaaat cgccatcccg aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa
    24541 tgatgtcgca gctatcccag ccaagttgat ccatctcttc acgagacatc ggcaaaaacg
    24601 gtgccggtcc aaaacaggcg gcccagtact gcggccagga gaacaggtcg cgatccggtt
    24661 ggatcaggga gatagagctc attttgcttc cagaaatgat aaaaaaataa tcaaaggccg
    24721 gggattataa gccggaacga aagagaaatc gaaaggtatt ccatactcgc cctcctcggg
    24781 cgagtatgaa gattacggta ccggattgac caacagttgc ccaatcgagc cacggtccgc
    24841 catttccagc gtctgactgt tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa
    24901 ataaacaagc agctccactt gtccgtcaac ccaaacggta tctttccagc ctctgtcttc
    24961 cgggaacggc atcgcgccat tcacgttacg gatctggaac attacgcctt caatatggaa
    25021 cgcttgcggc tcgtccgcgc gtaccgtcca gcgttcccac gttccttgct gcgcggtgac
    25081 atcaatacgg ttgacgtccc acagctgtcc attaataccc gggtcatcac ccagactgat
    25141 atcgcgactg cgaattggcg aaccagccat gatttcagtt ggcagcaagc gcatcggaag
    25201 actgtctgtg accagcggca gaaggccggt tgggcgcagc gttagcacca aggtagaaac
    25261 cagaatactg gatggctcaa agaagccgcg aatacgatca acaatgctcg ccgcttcacc
    25321 acaggtgatc gacacttcat cgccgttgct catatccacc agaatctcgc ggcgctcgcc
    25381 cggtgccagc gaaagttgct tcaccgatac aggagcaggc aggaatccct gatcgccaga
    25441 aatcacatgt aacgggcgac catcgctcat ctgtagttga tagcgacgag agttcgacgc
    25501 gttcagcagt cgcaagcgca cccagccacg cgagacttca acgtacgggc tttgtacacc
    25561 gttaaccagc agcgtatcac caacaaagcc gccgcttccc ggttcgttgt attctggcgt
    25621 accaaagtta tccagccgtt tatcctggat aatgaccgga aaatcatcca caccataatg
    25681 gttggggata ggcagcgact tgctgacttc atcttccacc agccacattc cggcaaggcc
    25741 gttatagacc tgctgagccg tgcggttggg agtattggcg tgataccaca gagtagctgc
    25801 gttctggcga ataggcagta cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg
    25861 accgcccatc agcgggcctg gtacctgtag cccggccacc gtcattgaga cattttctgt
    25921 caggcggttg ctgtaaataa gcttaacatc gtcgcccttc cagacgcgga tagtcggccc
    25981 caggtaacga ccattgattc cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg
    26041 cgcacgttgt acagtcataa acagcggttg cccacggcga gattcaagta gcggcggaac
    26101 gggtagcggt tgctgttgcc cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag
    26161 tgcaatcccc gatgcctgaa tgaactgacg ccgactgagt gacatatttg ctccatgtaa
    26221 aactggctaa agtaataccg tgcaggaaag tgttccccgc aaaaatacag attgccgact
    26281 taaacttttc cggcggcttc gcgttctgcg acttctttat cgagctcggc gattttttgt
    26341 tccattatcg aacgacaatg ggcagccagc tcacgaacct gatctttgcc atactgactg
    26401 acgtcaattg gcggcagcat ttcgacaatc accagaccgt tgtgcagtcg attaagatta
    26461 atcttattcg aagttgtaga gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc
    26521 gcgtgaaatg ctccagtctt gaacggtagc aggccgcgac cacggctgcg ggttccttcc
    26581 gggaacatcc agatggaaat acggcgtttt ttgaaatgat tcacgacttc cgcaatggtg
    26641 ccgtgagctt tagtgcgatt gtttctgtcg atcaataagt tgccggttaa ccagtacaac
    26701 tgcccgaaga aggggatcca cagcaagctc tttttaccta ccgtcaccgt cggcggttgc
    26761 acaatgttcg atgctgtcac catgtcatag ttgttctggt ggttagcgat atagatagca
    26821 ttgccgtagt tttcagcatc tgcaggtttg cggcactcaa ctttcaggcc gaacagtggc
    26881 gcaagacggc caaacatatg cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa
    26941 aggcagtaaa tggagccgaa tacacagact aagatgctgt aaatcacggt aataataaga
    27001 cgaaagatat atagcataac accctctgaa ggtctgagag actggcattg tacgtcacct
    27061 gaaatcaggt tagggcattg ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa
    27121 cgcatgaata aacaacggtt tcaagggaat ttttatcgtt tccgggggtt gaatacgctg
    27181 ccggatgacg acttaacgtt tcatccggcg ttccttgcta gcgggaggaa acagcgccct
    27241 ccccggcata ttactcttcg ctatcaccgc tgctggcacg gcgaggagag tcgatctcaa
    27301 cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt tcgccagtga
    27361 ctttctgtag ctcttccgga cgcagtttaa ttttgcgttt cccaacatga atggtcagcg
    27421 tgctttgcgg cggcagaacg tacagttgcg ccagaccatc ctcgccacgc gcggcttctg
    27481 ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc agatcactta
    27541 ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg gaagcatctt
    27601 caatcaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa gccttacccg
    27661 cacggttacg cgctaccaga tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag
    27721 ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc gccccaggcg
    27781 gcaacgttaa tttgccggtg agtggttcgc cctgaccacg cgccgacggc agcgtaatcg
    27841 ggtcgatggc atagctacga ccggtggaat caacaaacac taccggttgg ttactcttac
    27901 ctttcaccgc cgctttgaag ctatcacccg ctttataatt caggcccggc gcgtcgatat
    27961 catggccttt agcgctgcgt acccagccca tctgcgacag cacaatggtg acaggttcag
    28021 acggcagcat gtcgtgctcg ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac
    28081 gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc aggttattca
    28141 ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc agctcactct
    28201 gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc agttcgagga
    28261 tcgcttccgc ctgggtttcc gtaaggccaa accgcgacat cagcgccggt ttcggttcat
    28321 cttcgttacg aatgatctca atcacttcgt cgatgttgag aaacgccacc agcaaacctt
    28381 ccaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg cggcgcacgg
    28441 tatcgcgacg gaacaccagc cattcggaga ggatttccag caggtttttc accgccggac
    28501 gaccatccag accgatcata ttgaggttaa tgcgatagct cttttccaga tcggtggtag
    28561 cgaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc acaatcacca
    28621 gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc ggcagctttt
    28681 tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc acctgaaacc tgatgcggta
    28741 atgcgctgat aaccaccgcg ccatcttctt tcttccacac cgcgcgcata cgcactgaac
    28801 cacgtccgtt ctcgtagatt ttacgaatct cggcgcgcga agtgataatt tccgcttcag
    28861 tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtagtt ttcggctggt
    28921 cgattaatgc gattgccgcc tgagctactt cacgcaggtt atgcggtgga atatcggtcg
    28981 ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga cgggcaggta
    29041 gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc
    29101 ccagctcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg taacgcattg
    29161 ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca accagcggat
    29221 aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggcg ctatcgccgt
    29281 gcggatggta tttacccagt acgtcaccga cggtacgggc cgatttttta aatttggcgc
    29341 tggcattcag gcccagttca gacatcgcat acacaatgcg gcgctgaaca ggtttcagac
    29401 catcaccaat aaacggcaac gcacggtcca tgatcacgta catggagtag tttaagtagg
    29461 cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt aattctgatt
    29521 cctcaactta ttcgccagcc ttcaaagggg caatattgcc ggagatacta cattatctgc
    29581 cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc gacggcgata
    29641 aaggcatcaa tgtgccttga tatacaactc tttgctgtag acttgatcgg catagagggc
    29701 agcacaaggt tgcgcccatg ccacaccact cggcatgggg gtccgctccg ggcgattcga
    29761 gaagttgagg ttatgcgagt ccagggaagc ctaaagcatc aaagtagtcg actgtaagta
    29821 tacggttcag cccagtttag ctgtgtctcc accagtgccc cgcgcctcct gccataagtg
    29881 cggcttgctc atggatatcc ccgagacggc ttacggcgca ccggataata aatttcgcgt
    29941 atacatggta ttccttaccg gagaaattat aagcccaaca acaatacgcc tgtgacattg
    30001 ttcgctcaaa cttatcgcga gtgatttggc tcacactccg gtaaagattc cccatcacgc
    30061 caacggcaca taaacatccg tttccagtcg ctgctctgtc accccttcgg caagattggt
    30121 gtagtgaaac agaatcggtg ctttacgcat tttctcgccg cttgcaggca gccagtggcg
    30181 aataatgccc catatcgtgt gtgaaatatc gtccagctcg ccaacgtggc gggccacggc
    30241 ataacgtcca ccggtaagtt caccattgct cacaccataa cgattatcgg gaatcggttc
    30301 gctaacgctg ccgcagatat caaagcgaaa cgcttccggt gcggtggtgg caggggcgtc
    30361 ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa agccccgtct ctttacgcca
    30421 catgataaat ttcgccacac tgtagttgag caactcaggg ctacagcggt gcggcaacat
    30481 cgccacaggg attgatggga aatcgataat gtttacatcc agtgtcaggt ttgtcatttt
    30541 gcctccctgc gaaattattc ccgggaacgc gtcccgggag cggtaacaat tacggattta
    30601 ctttgcggat ctgtttgacg tcaatttcaa cagaattcca gtctttatcg acttcaccct
    30661 gaatctcaac cgtatctttc ggcgtcaccg tcacaccgtt ccagcgtttg tgatcgatat
    30721 caacattgat agtaccgctg gcatctttaa acacgtagag atcgtcagag atgcgttcaa
    30781 cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag ggattttgcg ctttctacag
    30841 tcgttacgct gccgttcggc ccctgaaatc ctccggcctg actttgcgtt accgatgggc
    30901 cagaaaaacc gccctgctct gctgccatca ccggtgcact gcacagggcc attactgcga
    30961 ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc cgagcattta
    31021 acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttaatgc tgtacagagc
    31081 gcgttacaac acggtttact ggcagcaaat acggttatcg cagggatgaa aaaatgcgaa
    31141 ttttactgat agaagatgac atgctgattg gtgacggcat caaaactggc cttagtaaaa
    31201 tgggttttag tgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg ctttatagcg
    31261 caccttatga tgcggtgatc ctggatttaa ccttaccagg catggatggt cgcgatattt
    31321 tgcgcgaatg gcgagagaaa ggtcagcgtg agccggtact gaacctgacc gcgcgtgatg
    31381 cgttagcgga acgtgtagaa gggctgcgtc tgggagctga cgattatctg tgtaaacctt
    31441 ttgcgttgat agaagtcgcc gccaagctgg aggctctgat gcgccgaacc aacggccagg
    31501 ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc cggcaaacgt atcgccacgc
    31561 tggctggcga acccttaaca ctgaaaccaa aagaatttgc cctgctggaa ttactgatgc
    31621 gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat acctgggacg
    31681 aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc aaactcggta
    31741 gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa tgaaatttac
    31801 ccaacgtctt agtctgcgcg tcaggctgac gctaatcttt ttaattctgg cctcggtgac
    31861 ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg atgaattgtt
    31921 cgacacccaa ctgatgctgt ttgccaaacg gttaagtacg ctcgatctca acgaaatcaa
    31981 cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacggtcatg ttgatgacga
    32041 tgcgctgacc tttgccatct ttacccacga cggcagaatg gtccttaatg atggcgataa
    32101 cggagaagat attccctata gctatcaacg ggaaggtttt gctgacgggc aactggtcgg
    32161 tgaagacgat ccttggcgtt ttgtctggat gacctcacct gatggcaaat atcgcatcgt
    32221 tgttggccag gaatgggaat accgtgaaga catggcgctg gcgattgttg ccgggcaatt
    32281 gatcccgtgg ctggtcgcac tgccgattat gttaatcatc atgatggtac tactgggtcg
    32341 tgaactcgcg ccgctgaaca aactggcgct ggcactacgt atgcgtgacc ctgactcgga
    32401 aaaaccacta aacgcgactg gcgtacccag cgaagtgcgg ccactggttg agtcgctaaa
    32461 tcaactgttc gcccgcacac atgcgatgat ggttcgagaa cgacgcttta cctccgatgc
    32521 agctcacgaa ctacgtagcc cgttaacagc gctgaaagtg caaaccgaag ttgcgcagct
    32581 ctctgacgat gatccgcagg cgcggaaaaa agcactgctc caattacatt ccgggatcga
    32641 tcgcgctact cgtctggttg atcaactgct cacgctatcg cggctggact cactggataa
    32701 ccttcaggac gtcgcggaga tcccgcttga agatctccta caatcgtcgg tgatggatat
    32761 ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg acactcaatg cccacggcat
    32821 caaacgcacc ggacaaccgc tattgctaag tttgttggtg cgaaatttgc tggataacgg
    32881 ccccggtgtg acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccacccgg
    32941 acaaaccgct accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaagctgca
    33001 tgacatgaat gttgagtttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg
    33061 gtaacttctc tgtctatgag agccgttaaa acgactctca tagattttac taatagcaaa
    33121 atataaaccg tccccaaaaa agccaccaac cacaaaccag acaaataacg agcgagaata
    33181 catatagcca aaactcgaac atttcccgca taaagagttt tcttaagata agaataataa
    33241 gtggcgtaag aagaaaaaat gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa
    33301 cggaaggtat actgcaaata ttgcctcccg actcaagcat ccagtcatac ttattgaaag
    33361 caaaaaaaga atctattaac caataagtaa gcattatata tagcaatgct tcaaatattg
    33421 ttttaatttt ctgctttaac atatatcagt acgctcataa aaatccattt ataattatca
    33481 acatgacacg ttgatatttt catcttttac ttattcaaag ccatattttc aatccccaaa
    33541 aaaaccgaac caaacgtgta aattattccg gtgcgagtaa gaattatgag gaatggctat
    33601 cagtattgtc attttcagaa aatatttatc ctgcatcggt gagtcagagc tagatcagac
    33661 ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat ttcacagtac cgctctgtga
    33721 cgtactatag tcgacaaacg tctcaccttg agattaaaaa atgagcaaca tcctgattat
    33781 caacggcgcg aaaaaattcg cccactccaa tggtcaactg aacgacaccc tgaccgaagt
    33841 cgcggatggc acactgcgcg acctcgggca tgatgtccgc atcgttcgcg ccgacagcga
    33901 ctacaatgtc aaagcggaag tacaaaactt tctctgggct gatgtggtga tctggcagat
    33961 gccaggctgg tggatgggcg cgccgtggac agtgaaaaaa tatattgatg atgtattcac
    34021 cgaaggtcac gggactctgt atgccagcga tggtcgtacc cgcaaagatc cgtcgaaaaa
    34081 atacggttcc ggcagcctgg tacagggcaa aaaatatatg ctttctctaa cctggaacgc
    34141 accaatggaa gccttcaccg aaaaagatca gttcttccac ggcgttggcg ttgacggtgt
    34201 gtatctgccg ttccataaag caaaccaatt cctcggtatg gaaccgctgc cgacatttat
    34261 cgctaatgac gtgataaaaa tgcctgatgt tccccgctat actgaagaat atcgcaagca
    34321 tcttgtggaa atttttggtt aactagagct caggctttag aaggagttaa ccatgcttac
    34381 cgtaatcgca gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcgg tattggatca
    34441 gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt tgccacggct atgcgccaat
    34501 ggtggattgc gctgctggcg tgagtttcca gtctatggca ccggattcta tcgtgatgat
    34561 tgagcagtgg gaaagcatcg cgcaccttga agcgcatctg caaaccccgc acatgaaggc
    34621 gtacagcgaa gccgtaaaag gcgacgtgct ggagatgaat atccgtattc tgcagccagg
    34681 gatttaatcc tgccttgttt gcccggccat cctgaccggg caatgttctt tcctttaaac
    34741 ctcaatctcc gccatgtcgc ctttctcttg caaccagttg cggcgatctt ccgagcgttt
    34801 cttcgccagc agcatatcca tcatcgcgtc agtacgctga tcgtcttcat catcgatagt
    34861 caactgcacc agacggcgag tgttcggatc aagcgtggtt tcacgcaatt gcatcgggtt
    34921 catttccccc agacctttaa aacgctggac gttcggcttg cctttcttgc gttttaattg
    34981 ctcaagtacg ccctctttct cttcttccgt cagcgcgtaa taaacctctt tcccgagatc
    35041 aatacggtag agcggtggca gtgcgacgta aacgtgaccg tgtttcacca acgcgcggaa
    35101 gtgttttacg aacaaagcgc agagcagcgt ggcaatgtgc agaccatcag agtccgcatc
    35161 cgcgaggata cagattttgc cataacgaag ctggctcaga tcgtcgctgt caggatcgat
    35221 accgatcgct accgaaatat catgcacttc ctgcgaggcc agcacttcgt cggaagaaac
    35281 ttcccaggtg ttaaggatct tacctttcag cggcatgatc gcctgatatt cacgatcgcg
    35341 cgcctgcttg gcagatccgc ctgcggagtc accttccact aggaacagtt cggtacggtt
    35401 aaggccctgc gcggtacaat cagccagttt gccaggcagc gccgggccgc tggtcagctt
    35461 tttacgcacc acttttttag ccgcacgcat acgacgctgg gcgctggaaa tcgccatctc
    35521 tgccagcaac tccgccgcct gaacgttctg gttcagccac aggatgaagg catctttcac
    35581 cacgcccgaa acgaatgccg cacactgacg cgaagagaga cgctctttgg tctgcccggc
    35641 aaactgcgga tcctgcattt taaccgacag aacataggcg cagcgatccc agatatcttc
    35701 cgccgacagc ttcacgccgc gcggcagaat gttgcggtat tcacagaact cacgcatcgc
    35761 gtccaacagg ccctgacgca gaccattaac atgggtaccg ccctgcatcg ttgggataag
    35821 gttgacgtag ctttcggtca gcagttcacc gccttccggc agccacagta gcgcccagtc
    35881 cacagcttca gtatcgccag cgaaattacc gataaacggt ttttctggca gcgtcggtaa
    35941 accgtttacc gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc agcgctgttc
    36001 ggtgttgttg atctcatctt taaaggtaat ctcaacgccg gggcacagta ctgctttggc
    36061 cttcagcaca tgcgtcaggc gtgaaacaga aaaacgcggg ctgtcaaaga aggtttcatc
    36121 cggccagaag tgcacgctgg taccggtatt gcgtttaccg caagtgccga caacctgtaa
    36181 atcctgcacc ttttcgccat tttcaaaggc gatgttataa acctgaccat cgcggcgcac
    36241 gttaacttct acgcgcttcg acaaggcgtt aactaccgaa atccccacgc catgcaggcc
    36301 gccagagaac tggtaatttt tgttagagaa tttaccgcct gcatgcagac ggcaaagaat
    36361 cagttcaacc gccggtactc cttcttccgg gtgaatatcc accggcatcc cgcgcccatc
    36421 gtcaatcact tctaacgact ggtcagcatg taagattaca tccacgcgtt ttgcgtgacc
    36481 cgccagtgct tcatcgacac tgttatcaat aacttcttgc cccaaatggt tagggcgagt
    36541 ggtatcggta tacatccccg gacggcggcg aaccggctca agcccggtga gtacctcaat
    36601 ggcatcagcg ttataagttt gcgtcatggt ttaagttagc aattcgagtt gatcgtcaga
    36661 gatggtgcag accaagaaaa tcgacgatcg ggttgaaata atcttcgaag cccgtgaatg
    36721 cgtggttgcc gccttctatg acagtctggc ggcaggaagc gtagtacgcc accgcctggc
    36781 ggtaatccag cacttcatct cccgtctgtt gcagcagcca gatcaaatcc ggcgcttcca
    36841 gcgggtcaat ctgcatgact ttgagatcgt aaatatggcg tgactctagc acatattgct
    36901 gcccggtgta ggggttctcg ttctgaccga gatagtccgt cagcaattca aacgggcgta
    36961 ccgccgggtt taccaccact gcgggcagca taaaacattg cgacaaccag gtggcgtaat
    37021 atccccccag tgacgaaccg acaataccca gcgaatcgcc gccatgttcc aggacaatgg
    37081 attccagcag ttctgccgcg tcggaaggat acggcggcaa ctgcggaatg atcatctcaa
    37141 cgtcagggtg atgttccgcc agccagtttt ttaacaagct cgcttttgca gagcgcggcg
    37201 agctgttgaa accgtgtaaa taaagaagcg tagacatcag tagccttctg aagcggtatc
    37261 aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc catcagcatg
    37321 taactcgaga gtacgccagc cgggcgcgat agtatccagc gtaaagttgg aacagtgcgg
    37381 cttaaactgc acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc agtcgagatc
    37441 cagctcctga tgaatatgac cgcacagcaa gtatttgacg tgcggaaact tcgccagcac
    37501 ggtatccagt tcgcccgcgt tacgcagact gtgttgatcg agccaactac aacccgcagg
    37561 tagcggatga tgatgcagca gcagcaacgt atggcgttct ggcgcatcgg ccagtttacg
    37621 ttccagccac tcaagctgaa actcgctcag ctcaccgtgc ggcacgccaa acacctggct
    37681 atccagcaac aggatttgcc attgctcacc aataaacacc cgcttcgccg gggagatacc
    37741 cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg tggttgcccg gcagccagac
    37801 gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc tgataggccg cagaggattg
    37861 atcctgcgct aaatcacctg tcgcgacaat caggtcgaat tcgtgctggt gtggacgaat
    37921 cgcctccagc accgcctggt agctttccca ggtgtttacc cctaacaggg cttcgtgctt
    37981 ttgtgcaaac aggtgagtat cggtaatttg taaaatcctt cctctggcct caccagccag
    38041 aggaagggtt aacaggcttt ccaaatggtg tccttaggtt tcacgacgct agtaaaccgg
    38101 aatcgccatc gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa actggttaat
    38161 ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga taatcatacc gcgctttgaa
    38221 gcgaaaaatc tgctggcttg aacacacttc agccaccatc gcgtcatgat acagacgcac
    38281 cgtcattgac ggaaggctcc agtaactgat cgcgggcgca gtctgttcta ttgtcactag
    38341 ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg ccacctgata
    38401 gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt gtgaaaagtt
    38461 catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca tttatgccca
    38521 ctcatttttt aacgcttgat gatgcagctg cagccattgc aaagcgatga ccgacgctgc
    38581 gttgtcgatt ttcccctctt ctacccactg gtatgcctgt tcccggctta ccacatgaac
    38641 gcgaatatct tcgttttcat cagccagacc atgaataccg cttgcggtcg tggcgtccac
    38701 ttcgcccacc ataattgacg aacgctcact ggtgcccccc gggcttgcca ggaaacttaa
    38761 caccggtttg gtccgtttga ctatcagtcc cgcctcttca atcgcttcgc gacgggcaac
    38821 atcttccaca ctttcaccct cttcaatcat cccggcaacc atctccagta gccagggggt
    38881 ttcgctggtg tcgtacgcgg caatccgaat ctgctcaatc agcacaactt catcacgcac
    38941 tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc gccgtacctc
    39001 atgactcatt tgcccgttga atagacgatg acgaaatcta taaagatcta atgaaaaaaa
    39061 gccgcgataa agtgtttctc gtgcaataat ttctacatcg tttttgccaa atgtaacggg
    39121 caggttgtct ggcttaagca ttgttaatgt cctagcacta atagtgaatt aaatgtgaat
    39181 ttcagcgacg tttgactgcc gtttgagcag tcatatgtta aattgaggca cattaacgcc
    39241 ctatggcacg taacgccaac cttttgcggt agcggcttct gctagaatcc gcaataattt
    39301 tacagtttga tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa gaaattgctc
    39361 cccattctta tcggcctgag cctttctggg ttcagttcgt tgagccaggc cgagaacctg
    39421 atgcaagttt atcagcaagc acgccttagt aacccggaat tgcgtaagtc tgccgccgat
    39481 cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc acagctaggt
    39541 ttaggtgcag attacaccta tagcaacggc taccgcgacg cgaacggcat caactcgaac
    39601 gcgaccagtg cgtccctgca gttaactcaa tccatttttg atatgtcgaa atggcgtgcg
    39661 ttaacgctgc aggaaaaagc agcagggatt caggacgtca catatcagac cgatcagcaa
    39721 accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat tgacgttctt
    39781 tcctatacac aggcacaaaa agaagcgatc taccgtcaat tagatcaaac cacccaacgt
    39841 tttaacgtgg gcctggtagc gatcaccgac gtgcagaacg cccgcgcgca gtacgatacc
    39901 gtgctggcga acgaagtgac cgcacgtaat aaccttgata acgcggtaga gcagctgcgc
    39961 cagatcaccg gtaactacta tccggaactg gcggcgctga atgtcgaaaa ctttaaaacc
    40021 gacaaaccac agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa cctgtcgctg
    40081 ttacaggcac gcttgagcca ggacctggag cgcgagcaaa ttcgccaggc gcaggatggt
    40141 cacttaccga ctctggattt aacggcttct accgggattt ctgacacctc ttatagcggt
    40201 tcgaaaaccc gtggtgccgc tggtacccag tatgacgata gcaatatggg ccagaacaaa
    40261 gttggcctga gcttctcgct gccgatttat cagggcggaa tggttaactc gcaggtgaaa
    40321 caggcacagt acaactttgt tggtgccagt gagcaactgg aaagcgcaca tcgtagcgtc
    40381 gtgcaaaccg tacgttcctc cttcaacaac attaatgctt ctatcagtag tattaacgcc
    40441 tacaaacaag ccgtagtttc cgcgcaaagc tcattagacg cgatggaagc gggctactcg
    40501 gtcggtacgc gtaccattgt tgatgtgttg gatgcgacca ccacgctgta caacgccaag
    40561 caagagctgg cgaatgcgcg ttataactac ctgattaatc agctgaatat taagtcagcc
    40621 ctgggtacgt tgaacgagca ggatttgctg gcactgaaca atgcgctgag caaaccggtt
    40681 tccactaatc cggaaaacgt tgccccgcaa acgccggaac agaatgctat tgctgatggt
    40741 tatgcgcctg atagcccggc acccgtcgtt cagcaaacat ccgcacgcac taccaccagt
    40801 aacggtcata accctttccg taactgatga cgacgacggg gcttcggccc cgtctgaacg
    40861 taaggcaacg taaagatacg ggttatctgc cgcattcttc ccccttctcg cttcaatttc
    40921 gaccagccat cctctattct gatgggtatt taccactggt cccggaagac aaaaatgaaa
    40981 cggacaaaat ccatacgcca cgcatcgttc cgcaaaaact ggagcgcacg ccatctgaca
    41041 ccagtcgctc tcgcggttgc cactgttttt atgctggctg gctgtgaaaa gagtgatgaa
    41101 acagtgtctc tctatcaaaa tgctgacgac tgttcagctg caaacccagg caaaagcgcc
    41161 gaatgtacca ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc gccgaaatac
    41221 gccacccgtg aagactgtgt tgctgaattt ggtgaaggtc agtgtcagca agcaccagcc
    41281 caggctggca tggcaccaga aaaccaggcg caggctcagc aatccagcgg gagtttctgg
    41341 atgccgctga tggccggtta catgatgggg cgtctgatgg gcggcggcgc gggatttgca
    41401 cagcagccgc tgttctcctc gaaaaacccg gccagtccgg cttacggtaa atataccgac
    41461 gcgacgggta aaaactatgg cgcagcccag ccaggccgca ccatgaccgt accgaagacg
    41521 gcaatggcac caaaaccggc gaccaccact accgttaccc gtggcggttt tggtgaatct
    41581 gttgccaaac aaagcactat gcagcgtagc gcaaccggca cctcttctcg ttcaatgggt
    41641 ggctgatacc gatggaaaga gtcagtatta ccgagcgccc ggactggcgt gagaaagccc
    41701 acgaatacgg tttcaatttt cacaccatgt acggcgagcc gtactggtgt gaagatgctt
    41761 actacaagtt gaccctcgcc caggttgaaa agctggaaga agtcaccgcc gaactgcacc
    41821 agatgtgcct gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg accaaattcc
    41881 gcataccaaa acacacctgg agttttgtgc gtcaatcatg gctgactcac cagccatcgc
    41941 tttattcgcg tcttgatctg gcgtgggatg gcactggcga acctaaactt ctggaaaata
    42001 acgccgatac gccaacgtca ctatacgagg cggcgttctt tcagtggatc tggctggaag
    42061 atcagcttaa cgccggtaac ttgtcggagg gcagcgacca gtttaacagt ctgcaagaaa
    42121 aactgatcga tcgcttcgtt gagctgcgtg aacagtatgg cttccagttg ctgcatctca
    42181 cctgctgtcg cgacacggtg gaagatcgcg gaaccattca gtatttgcag gactgcgcaa
    42241 ccgaagctga aatcgccacc gagttcctct acatcgacga gataggttta ggcgaaaaag
    42301 gccagttcac ggatttacag gatcaggtga tttctaacct gttcaagctt tatccgtggg
    42361 aatttatgct gcgcgaaatg ttctccacca agctggaaga tgctggcgta cgctggctgg
    42421 aaccggcgtg gaagagcatt atctccaaca aggcacttct accgctactg tgggagatgt
    42481 tcccgaatca cccgaacctg ctgcccgctt attttgcgga agatgatcat ccgcaaatgg
    42541 aaaaatatgt ggttaaaccg atcttctccc gtgaaggcgc aaacgtgtcg atcattgaga
    42601 acggcaaaac cattgaagca gcggaaggtc cgtatggcga agaagggatg attgttcagc
    42661 aattccaccc gttaccgaaa ttcggcgaca gctatatgct gattggtagc tggctggtga
    42721 acgatcaacc tgccggaatt ggcattcgtg aagaccgtgc gttgatcacc caggatatgt
    42781 ctcggtttta tccacatatt tttgttgaat aagccacgat accggatggc actcgccatc
    42841 cggtaattgt tagcctatct gcaccgacag catacttaaa ctgcccattt ctataccatc
    42901 gaccggaatg gtaattggct cctgcccatc ccacacacct aacacataca acaacggcag
    42961 atagtgctct ggcgttgggt tcgataacgc gccaccttca tggtcgagat aattcaccag
    43021 aggatgctgc tctaccggcc cttgccacgt cagattcgct ttcacatact cattaaacga
    43081 cgtcgcccac ggatacggtg aactatcacc gtgccacttc actgtgcgca ggttatgcac
    43141 cacgttaccg ctggcgacca acattattcc ttcatcacgc agcgccgcga gtttgcgccc
    43201 catttcgaaa tgccaggcga caggtttgct actgtcgata ctcaactgca ccatagggat
    43261 atcggcgtca ggatacatct taatcagcac gccccacgag ccgtggtcaa agccccaggc
    43321 ttctttatcc agcgtcaccg ggatcggcgc taacagctca accagacgct gtgccagcgc
    43381 aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc
    43441 atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa accagtgagc
    43501 cgaaaccacc acaatcgctt gcgggcgtgg caatgtcatc cccaacttct gccagctgcg
    43561 ggtatacaaa ttatcttcca gcacgttcat cggactaccg tgacctaaaa acaatgctgg
    43621 catacgtgtt gaagacatga tgatatcctt aactaaaggt gtcattttga tatcctcaca
    43681 atacgcttgt tcggcggagt aagaacccgg ataacaatga tgatgatcat cagttatttt
    43741 gacgatctgc ctgaaggtga agatttataa ggagttgtcg atgtcagtac ctctcattct
    43801 gaccatactg gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc tcggacaaaa
    43861 accctcgaac cgcttactgg cgttttcgct aggttttgcg gcggggatca tgttgctcat
    43921 ctcattaatg gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc cggtgttggg
    43981 ttatggaatg tttatcttcg gtctgcttgg ctactttggc ctggaccgca tgttgccgca
    44041 tgcgcatccg caggatttaa tgcaaaaatc ggtgcagctg ttgccaaaat cgatcaagcg
    44101 cacagccatt ctgctcactc tcggcatcag tctgcataac ttcccggaag ggattgccac
    44161 ctttgtcacg gcgagcagca acctggagct gggatttggc atcgcactgg ccgtcgcgtt
    44221 gcacaatatc cctgaaggtc tggcagtggc aggcccggtt tatgcggcaa cggggtctaa
    44281 acgcaccgcg attctgtggg cggggatttc tggactagca gaaattcttg gtggtgtgct
    44341 ggcgtggtta atcctcggta gcatgatttc cccggtggtc atggcggcaa tcatggcggc
    44401 ggttgcagga attatggtgg cgctctcggt tgatgaatta atgccgctcg ccaaagagat
    44461 tgaccctaat aataacccca gctatggcgt actatgtgga atgtcagtga tgggattcag
    44521 tttagtgctg ctacaaacgg cgggaattgg ttaaaaacaa catcagaagt tgtccagcac
    44581 atggacaact tctgccagct tcacgaaagc cttattctac cttttcggct gtctcttctc
    44641 tcgtactgtt taagtatttg ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg
    44701 cccgacaata acgtttcaaa tgcaggaaat aacccaccac aaccagagca atcaatacaa
    44761 tccagtacca ggcgataaac atccttttac ccttaatatt taacaatact aaacatttag
    44821 cgcataaatt tcacatatcc tttttcggat atattcatat ggtcgcataa taacggaaca
    44881 attatatgat tttgatcata atgaaaaaga atgcctgata ttaatgaaat tttctcatag
    44941 aaagagaagc ggaattaaca tcttgcatag cagggcaaaa taaaaaccga agccagctac
    45001 aacttcggtt tcttgattaa ggcagtaatt aagcagcggt tttcagctgg ctttacgctc
    45061 atgtgcctga cggtatgcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt
    45121 ggcaaactca atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca
    45181 cagcacacca gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg cttcagtatg
    45241 accgccacgc gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt ggcccgggcg
    45301 attcagatca gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taatacgatc
    45361 agcggcagaa acaccggtag tcacaccttc agccgcttca atggtcacgg taaaaccggt
    45421 gccataggcg ctggtgttat tttctaccat cattggcaga tcaagttgtt tacggcgatc
    45481 ttcagtgatg cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc
    45541 aacggtcatg gtttctgccg ggaagatcat atcgccttcg ttttcacggt cttcatcatc
    45601 aagcaccatt acaccgcgtc cttcacgcag cgcagccagt gcattttcaa cacgttcgaa
    45661 aggcgtacca aaagaggaaa gtagcgtctg attcatggta aaaaaacctc actaaaatta
    45721 tggttaccag aatcagggca gtcttaggag tggcggcata tagccaaaat aacgtgagca
    45781 ggtccatgcc cgacagaatc gttactctct cccatccgga ctctaaccgt cggccccgga
    45841 attacaccgg atctgctgtc ctttgagttc gcacccaaag cgctcgcggg ctttcaactg
    45901 agttgattta ccgccggtgg ggaatttcgc cccgccctga gaataagcgg attcactata
    45961 acgctaatga ttagcggcag caacgcatag cttcacataa ttctggttta tgacttaccc
    46021 ttatcgcact acaatggtac tcaacaccta tcagtacagg gaaactacaa tgattgaccc
    46081 gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag gaatcaggga
    46141 gttcggggaa gatgtggaga aaaaaatccg ccaaacccta caagctcagc tgacgcgcct
    46201 cgaccttgta agccgtgaag agttcgacgt ccaaacgcaa gtcctgttac ggactcgtga
    46261 gaaactggcg ctgcttgaac aaagaatcag cgaactggaa aatcgcagta ctgagatcaa
    46321 aaagcagcca gatccagaga ctctcccccc aacgctgtaa acatttgtct ttaatgcact
    46381 gtaaattatg aattaatcct ctaatttaca gtgttttcta ttacattatc gcttaatccc
    46441 attcagagta tcatcacaaa aatgaatgat tgcaattgaa aatataattg ttatgtattc
    46501 tttaagtttt acttaatcga tatttagact ggccc
//
LOCUS       DAECNO010000023        45805 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.023, whole genome shotgun sequence.
ACCESSION   DAECNO010000023 DAECNO010000000
VERSION     DAECNO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.023"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            114..2267
                     /locus_tag="KKO08_001285"
     CDS             114..2267
                     /locus_tag="KKO08_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBD6317606.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2320..4050
                     /locus_tag="KKO08_001286"
     CDS             2320..4050
                     /locus_tag="KKO08_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317607.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="KKO08_001287"
     CDS             complement(4110..4601)
                     /gene="yajQ"
                     /locus_tag="KKO08_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBD6317608.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4769..5680
                     /gene="panE"
                     /locus_tag="KKO08_001288"
     CDS             4769..5680
                     /gene="panE"
                     /locus_tag="KKO08_001288"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBD6317609.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5643..6233
                     /gene="yajL"
                     /locus_tag="KKO08_001289"
     CDS             5643..6233
                     /gene="yajL"
                     /locus_tag="KKO08_001289"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBD6317610.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="KKO08_001290"
     CDS             complement(6287..7735)
                     /gene="thiI"
                     /locus_tag="KKO08_001290"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBD6317611.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7941..8183
                     /gene="xseB"
                     /locus_tag="KKO08_001291"
     CDS             7941..8183
                     /gene="xseB"
                     /locus_tag="KKO08_001291"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBD6317612.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8183..9082
                     /gene="ispA"
                     /locus_tag="KKO08_001292"
     CDS             8183..9082
                     /gene="ispA"
                     /locus_tag="KKO08_001292"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBD6317613.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9107..10969
                     /gene="dxs"
                     /locus_tag="KKO08_001293"
     CDS             9107..10969
                     /gene="dxs"
                     /locus_tag="KKO08_001293"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBD6317614.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11024..11941
                     /gene="yajO"
                     /locus_tag="KKO08_001294"
     CDS             11024..11941
                     /gene="yajO"
                     /locus_tag="KKO08_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBD6317615.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="KKO08_001295"
     CDS             complement(12052..12570)
                     /gene="pgpA"
                     /locus_tag="KKO08_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBD6317616.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="KKO08_001296"
     CDS             complement(12548..13525)
                     /gene="thiL"
                     /locus_tag="KKO08_001296"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBD6317617.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="KKO08_001297"
     CDS             complement(13603..14022)
                     /gene="nusB"
                     /locus_tag="KKO08_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBD6317618.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="KKO08_001298"
     CDS             complement(14042..14512)
                     /gene="ribE"
                     /locus_tag="KKO08_001298"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBD6317619.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="KKO08_001299"
     CDS             complement(14601..15704)
                     /gene="ribD"
                     /locus_tag="KKO08_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBD6317620.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="KKO08_001300"
     CDS             complement(15708..16157)
                     /gene="nrdR"
                     /locus_tag="KKO08_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBD6317621.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16308..16847
                     /locus_tag="KKO08_001301"
     CDS             16308..16847
                     /locus_tag="KKO08_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD6317622.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17145..18029
                     /gene="tsx"
                     /locus_tag="KKO08_001302"
     CDS             17145..18029
                     /gene="tsx"
                     /locus_tag="KKO08_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBD6317623.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18206..18553)
                     /locus_tag="KKO08_001303"
     CDS             complement(18206..18553)
                     /locus_tag="KKO08_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBD6317624.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18682..19653)
                     /gene="secF"
                     /locus_tag="KKO08_001304"
     CDS             complement(18682..19653)
                     /gene="secF"
                     /locus_tag="KKO08_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBD6317625.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19664..21511)
                     /gene="secD"
                     /locus_tag="KKO08_001305"
     CDS             complement(19664..21511)
                     /gene="secD"
                     /locus_tag="KKO08_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBD6317626.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="KKO08_001306"
     CDS             complement(21539..21871)
                     /gene="yajC"
                     /locus_tag="KKO08_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBD6317627.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="KKO08_001307"
     CDS             complement(21894..23021)
                     /gene="tgt"
                     /locus_tag="KKO08_001307"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBD6317628.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23076..24146)
                     /gene="queA"
                     /locus_tag="KKO08_001308"
     CDS             complement(23076..24146)
                     /gene="queA"
                     /locus_tag="KKO08_001308"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBD6317629.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24239..24812
                     /gene="acpH"
                     /locus_tag="KKO08_001309"
                     /pseudo
     CDS             24239..24812
                     /gene="acpH"
                     /locus_tag="KKO08_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="KKO08_001310"
     CDS             complement(24817..26634)
                     /gene="malZ"
                     /locus_tag="KKO08_001310"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBD6317630.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26790..28163)
                     /gene="proY"
                     /locus_tag="KKO08_001311"
     CDS             complement(26790..28163)
                     /gene="proY"
                     /locus_tag="KKO08_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBD6317631.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="KKO08_001312"
     CDS             complement(28239..29558)
                     /gene="brnQ"
                     /locus_tag="KKO08_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBD6317632.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="KKO08_001313"
     CDS             complement(29965..31260)
                     /gene="phoR"
                     /locus_tag="KKO08_001313"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBD6317633.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="KKO08_001314"
                     /pseudo
     CDS             complement(31318..32007)
                     /gene="phoB"
                     /locus_tag="KKO08_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            32197..33399
                     /gene="sbcD"
                     /locus_tag="KKO08_001315"
     CDS             32197..33399
                     /gene="sbcD"
                     /locus_tag="KKO08_001315"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBD6317634.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33396..36539
                     /gene="sbcC"
                     /locus_tag="KKO08_001316"
     CDS             33396..36539
                     /gene="sbcC"
                     /locus_tag="KKO08_001316"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBD6317635.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36665..37846
                     /gene="araJ"
                     /locus_tag="KKO08_001317"
     CDS             36665..37846
                     /gene="araJ"
                     /locus_tag="KKO08_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBD6317636.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37989..38897)
                     /gene="mak"
                     /locus_tag="KKO08_001318"
     CDS             complement(37989..38897)
                     /gene="mak"
                     /locus_tag="KKO08_001318"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBD6317637.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVTIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39022..39933
                     /gene="rdgC"
                     /locus_tag="KKO08_001319"
     CDS             39022..39933
                     /gene="rdgC"
                     /locus_tag="KKO08_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBD6317638.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(40011..40466)
                     /locus_tag="KKO08_001320"
     CDS             complement(40011..40466)
                     /locus_tag="KKO08_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317639.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40463..40798)
                     /locus_tag="KKO08_001321"
                     /pseudo
     CDS             complement(40463..40798)
                     /locus_tag="KKO08_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="KKO08_001322"
     CDS             complement(40953..41237)
                     /gene="ppnP"
                     /locus_tag="KKO08_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBD6317640.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41309..41986)
                     /locus_tag="KKO08_001323"
     CDS             complement(41309..41986)
                     /locus_tag="KKO08_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBD6317641.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="KKO08_001324"
     CDS             complement(42247..42435)
                     /gene="yaiA"
                     /locus_tag="KKO08_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBD6317642.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="KKO08_001325"
     CDS             complement(42485..43009)
                     /gene="aroL"
                     /locus_tag="KKO08_001325"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBD6317643.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43192..43650)
                     /locus_tag="KKO08_001326"
     CDS             complement(43192..43650)
                     /locus_tag="KKO08_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBD6317644.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43770..44579
                     /gene="proC"
                     /locus_tag="KKO08_001327"
     CDS             43770..44579
                     /gene="proC"
                     /locus_tag="KKO08_001327"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBD6317645.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="KKO08_001328"
                     /gene_synonym="dgcC"
     CDS             complement(44596..45711)
                     /gene="adrA"
                     /locus_tag="KKO08_001328"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBD6317646.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11911 a        11943 c        11203 g        10748 t
ORIGIN      
        1 taagttggca gcattacctt cgcttaattc catataactg gacgattaca ttagaagtaa
       61 actatacttt ttatgaaata tcagggataa attattcaca tggaaaatac atcatgtaca
      121 aatcattatt tactattatc attctactgt atagcgccag ttcatatgca aaaagcgaat
      181 cccctcccgc aagcactata actttcattg cacaaatcga tcatgttgag ctaaataaaa
      241 cagcattatc tcatgatatg aaactcgttc ttgccgatcg tttttatttc aaaaccagta
      301 atccttgcga aacaaattca atttctgctc gaccagaatc aatggatatt actgacaaag
      361 cctatgccga caaattaaaa aaattagaaa ctccttttct tgatgatcgc tatttcttta
      421 tgaccatcaa tcagtgtgac ggtaatggca ctcccatgct gacagacata aaagtatgta
      481 caacagaatt atgtggtgct gaatatacaa agaaaatgtc tactgtatgg ctcgatgaaa
      541 ctttaaaacc aactgctaaa cgtctggcaa tttattttat tcaatctcct ttaccctatg
      601 acgaggagaa aaaacaatgg aaagtcaccg gatggtatat ctcatcaact gataatcagt
      661 cttcaactaa acagattgca tttgaaggat atacagacac agataacctc tccgattcta
      721 aatttatttc ttcatttaaa tcctggtttg aaagtggaaa gataaaaaac attgctaact
      781 ataatccaaa aggagaaaga gatggaaaat acgaaagtta ttacaatagc aatggtaacg
      841 tctatgaatc tctaagtttt tctaatggtc ttatcaacgg tgagtttatt acatattatg
      901 aaaatggggc cattgaaagt aaacgtcatt ttaataacga caaaattaaa gatggtgaat
      961 gccctcattt ttatgaagat gggaaaatca aacagcaaca tagctatctg aatagtaaac
     1021 tcgatggtcc tgcatatgaa tattatcctg atggcaaact catgcaggaa aatttctatc
     1081 agcaaagtgt gttgattgga aacgatacca gttactacca atccggaaaa ataagctcta
     1141 tacacaatag aaattcccgt ggtcagtatg atggagctaa cgaacgttat agtgaagagg
     1201 gtaagttact gtctaaatcc gtctataaag acggaaaaca aatttctgtt caaacctggt
     1261 atgaaaatgg gcagaaagaa gaggagaaac attttgatga gcaaggccaa ttaaatggtc
     1321 tggttaagca atggtataaa aatggtaatc ttgcaaaatc acaaaactat aaacatgaca
     1381 tactcgatgg taacagtgaa gagtggtacg aaaatggcat ccctgaaagt ttgtaccctt
     1441 ataagaatgg aaaaaccgac ggcattgcca aatcatggaa taagtatggt aaattaacct
     1501 attcaatcga atacaaaaat ggcgtagaga atggggcata tcgtaactgg agcaaaaaca
     1561 caggtaaact aaccaaagaa accctgtacg tcaatggtat cagacaggga gtggaaaaag
     1621 agtttaacga tcgcacaggt aaactattaa cctccacgca atatgttaat aataaaagac
     1681 atggtacgga ggaaacttat gatcaaaacg gaataaaata tattacatgc tatcaaaatg
     1741 atcagaaatt atcgtcttta gacaatccaa cacaaataaa agataatgca acaacaggcg
     1801 attccagtgc tcaatttgca ttagggaaat acgaattcat ctgtgcaaat atagatgaag
     1861 ggattaaatg gttaaccaaa tctgcagagc aaaaaaacac tgatgcgata tactttttag
     1921 ctacagccta caaaggcaat ggtatacctg caaacaatga aaaataccta gcatatctgc
     1981 aacaagctgc aacactgggc agtagtaatg cccaggcgga aattggctat ttatatctca
     2041 tcggaaaaga actaccacag aatcttcctg atgctggtgt ctggttcaaa aaagcagctg
     2101 cacaaggtaa ttttgccgca catttctatc tgggaaggat gtatcagaat ggtgacggcg
     2161 ttgagagaaa tatggaaaaa gccaggtttc atctatctaa tgcggcagaa ggtggaatag
     2221 aacctgcgct taaagcatta aatgaacttg aacatcaaac aaaataaagc gctcagggac
     2281 cacgtaaggt tcatcaaaca gaaaaggata tagtttgcga tgaaagcgaa atctactttg
     2341 ttattaatga ttttatctgt tttttattct catcaggttt tatccagcga acccactcag
     2401 ccggatgttt attctgtcgt cgagaaaaaa ctaaactctg ctttacctct ggaagaaaat
     2461 accaccttca gatcgcaggc tgaatggttt aatttgcaat atgaattatt agtatctggc
     2521 taccctgaac gtgcttacaa tttactgagc aaacttgaga cagaatcgaa aaacgctaaa
     2581 tattatctgg atttaactgc ctccagagtc gctgaagaag aagcaccaga gacagcctta
     2641 acttttttat caaaaattgg gttaaataaa ccggctactc ttacatcctt tgagagctat
     2701 atcaatagct ggataaaaag caaacaaccc gaaaaggcta tgcagcttct cagcctcgat
     2761 gagaatgccc tgaattatta tttaccgact gtgttagtcg catatcagat cgttcctgat
     2821 aaggcaataa gtatttataa ttctgtatat ggagataatg ttgttatacc ctatcagcaa
     2881 gtcaccatgt tacttacagt tgcggagggt tatcaaacaa aaggcgatat aaaaaacgcc
     2941 attcattatg ccgataaggc gctgaatatg tttgataaag ctattgctgt agatagcagt
     3001 cgtgaatccc attatagtga tgagtatttg aaactcatga atatctacgc cgccaatggc
     3061 aacaaagaaa aagcggtggt tttatctcaa cgtctacaca aagccattac cgaatctcaa
     3121 tcgaattact taagtactct ggaaggaatt ttattgtttt atagaaataa tgatatgcag
     3181 caagactatc aaaacagcct ttcaaattac atagtattta tcgataaaat ttttgccttt
     3241 tccccatcaa caagaagtga attaagcctc attaatttat taaattcatt aaatgaggtg
     3301 gaattgatga gaaaacgtct ggcgtttctg atgacagcac cagaatacgc ttgttatgat
     3361 agtcagtatt gttatgaaga caaagtcagc gcattaaaaa tcctgtatcg tcatcatgac
     3421 aatgagctaa tggacaaata tttcacgact cttcttaaag agatcagtaa tcaaccatta
     3481 gaagactggg atataataat aagtaatact ggtgaaaaat ttagtgaagt gggtctaacc
     3541 aaatatgctc aacatctcgc atctttcgct gaagaatttt atataaaaga gcaaaaatca
     3601 cattctgaaa gtgaaatgag aggactattt tcatcacttg ccgaattata tagttttggt
     3661 aatgatactg ccagcgcaaa ccgagtgttc catcagcatg tccctgcctt caatacagat
     3721 catatgattc aatattttat aaatgccaaa gaatggaaca atgccaggga attattgata
     3781 aaagaggaaa tgttgggtat gcataatctg atattgttgg agaatatttg tatgcaaaaa
     3841 aatgcagaat gcatggcaca cattaccttt acgcttaata aattaacaac acaaccagca
     3901 ataacaaaaa tagatagcgt gggtaatgag caactttatc agattggcaa aatctaccat
     3961 aagttggaaa ttaaacctga gccagagcaa caggttttaa ttcagtcgct ttatgaccgg
     4021 gcatcaggaa gcgcctctgc aactcaataa atgcatggtc aaaagggcca ccacggtggc
     4081 cctgacaatc agtagctata tccagaaagt taatcgcgga agtttttgaa ctggaacggc
     4141 tgaccgagat cgccaccgcg caccatcgcc atgacagcct gcaaatcatc acgagatttg
     4201 ccagtcacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg
     4261 atcatcttga cgattttttt ctgagtggcg ctctcaatgc cctgtttcag tttcgcttcc
     4321 acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga actgccttca
     4381 atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac ctggaagtcg
     4441 gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg
     4501 ttacggaagt caaaacggga ctccacttcg cgactcgcgt tatcgaccgc gttacgtgct
     4561 tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt
     4621 tttgatgcga agcataatac ctgcaaagtt aaataaccaa cagtcctgca ccaggacgta
     4681 tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac
     4741 ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt
     4801 agggcaatta tggcttacag cactttgtaa acagggtcat gaagttcagg gctggctgcg
     4861 tgtaccgcaa ccttattgta gtgtgaatct ggttgagaca gatggttcga tatttaacga
     4921 atcgctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc tggtgacgct
     4981 gaaagcatgg caggtttccg atgccgtcaa aagcctcgca tccacactgc ctgtaactac
     5041 gccaatactg ttaattcaca acggcatggg caccatcgaa gaattgcaaa acattcagca
     5101 gccattactg atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca
     5161 tgtggcaaac ggaatcacgc atatcggtcc ggcacggcaa caggatggcg attacagtta
     5221 tctggcggat attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc
     5281 cgagctgtgg cgcaagctgg ccgtcaactg cgtgattaat ccactgactg ccatctggaa
     5341 ttgcccgaac ggtgaattac gtcatcatcc gcaagaaatt atgcaaatat gcgaagaagt
     5401 cgcggcagtg atcgaacgcg aagggcatca tacttcagcg gaagatttgc gtgattacgt
     5461 gatgcaggtg attgatgcca cagcagaaaa tatctcgtcg atgttgcagg atatccgcgc
     5521 gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca
     5581 tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga
     5641 atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca
     5701 ccacgatcaa cctcttggtt cgcggcggta tcaaagtcac cactgccagc gtcgacagcg
     5761 atggcaacct ggcaattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg
     5821 tcgaagtggc tgatggcgaa tatgacgtga tcgtgctgcc tggtggcatt aaaggcacgg
     5881 agtgttttcg cgatagcact ctgctggttg aaaccgttaa acagttccac cgttccgggc
     5941 gtatcgtcgc ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatctttc
     6001 cgattggcaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc
     6061 aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggta
     6121 cagctatcga ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg
     6181 aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga
     6241 tgcggcaaac gtcgcatctg accagatgcg acgtaaaaac tacaaattac gggcgatata
     6301 ccttcacatt gttaaagcct tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc
     6361 cgcgctcaca ccacagcagc caggttttgt tctggtcgag atcgccaaat ttggtgctca
     6421 gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc ttatcttcct
     6481 gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagccg ttgacggttt
     6541 ccacttccac cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg
     6601 cttcctcaac cactttatcg agaatactga agtcgaactt ctcttcttcc gcttcaatct
     6661 tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat tccggcatcg
     6721 tgcgagcaaa gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt
     6781 aagaaatcag cggacgcagg atcagcgtgt cagagacgtt atcaatcagg cgcaggttgg
     6841 tcagcgtctg actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt
     6901 aacgttcagc cactttagat gcggcacgca ccatcatacg tttgaggata acgcccatct
     6961 gaccgtcgtc gattttctcg agaatttccc cgacaaccgg ttcgaaatta atggcgacaa
     7021 aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc tgacgcacgc
     7081 caatttcatg cgctgcgccg ccgaggttaa agaagcagta atgcacgcgg cagccgcgac
     7141 gcatcaacat ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat
     7201 cttcctgggt gccaatcggg aaaccaccaa taccttcgta gcgaccttta atcagcagga
     7261 ggcgatcgtc ttccacttcc agatgaacag ttacatccgg gttagtcagc ttcacgcgcg
     7321 cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa
     7381 aatcatgttt gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc
     7441 gatactgaac caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga
     7501 cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata gtcagacgct
     7561 ggttttcatc tttcgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct
     7621 catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc
     7681 gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg
     7741 caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc
     7801 tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct
     7861 taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata
     7921 ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc tttgaaaagg
     7981 cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag
     8041 aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac
     8101 aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc
     8161 cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca
     8221 ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt
     8281 cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt tcctggttta
     8341 tgccaccggt catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgctgccgt
     8401 agagtgtatc cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga
     8461 tctgcgtcgc ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc
     8521 tggcgacgct ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt
     8581 gtcggatcgc gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg
     8641 aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc
     8701 gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg
     8761 tgcattaagc gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga
     8821 gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc
     8881 aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact
     8941 tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcaggc
     9001 gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc tagcggacta
     9061 catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat
     9121 tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa
     9181 agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg
     9241 ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta
     9301 tgtctacaac accccatttg accaattgat ttgggatgtg ggacatcagg cttatccgca
     9361 taaaattttg accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca
     9421 cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac
     9481 ctccatcagt gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaattgccg
     9541 caccgtctgt gtgattggcg acggcgcgat taccgctggc atggcgtttg aagcgatgaa
     9601 tcacgcgggc gatattcgcc ctgatatgct ggtggtcctc aacgacaatg aaatgtcgat
     9661 ttccgaaaat gttggcgcgc ttaacaacca tctggctcag ctgctttccg gtaagcttta
     9721 ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccca ttaaagagct
     9781 gctcaaacgt accgaagaac atattaaagg catggtggtg cctggcacgt tgtttgaaga
     9841 gctgggattt aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac
     9901 gctaaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg
     9961 tcgtggttat gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga
    10021 tccctccagc ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt
    10081 tggcgactgg ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc
    10141 gatgcgtgaa ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga
    10201 cgtggctatt gccgagcaac acgcagtgac ctttgccgcc ggtctggcga ttggtggata
    10261 caaacccatt gtcgcgatct actccacctt cctgcaacgc gcctatgatc aggtgctgca
    10321 tgacgtggca attcaaaagc taccggtcct gttcgccatt gaccgcgcgg gcattgttgg
    10381 tgctgacggt caaactcacc agggcgcttt tgatctctct tacctgcgct gcataccaga
    10441 aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta
    10501 tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcgg ttggcgtgga
    10561 actgacgccg ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa
    10621 actggtgatc cttaactttg gtacgctgat gccagaaacg gccaaagtcg ccgaatcgct
    10681 gaacgctacg ctggtcgata tgcgttttgt gaaaccactt gatgaagcgt taattctgga
    10741 aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc
    10801 aggtagcgga gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat
    10861 tggcctgccg gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg
    10921 cctcgatgcc gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca
    10981 ctcctgctat gcttaagaaa ttattcatgg caggagtggc agcatgcaat acaacccctt
    11041 aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc tgtatgacct ttggcgagcc
    11101 agatcgcggt aatcacgcat ggacactgcc ggaagagagc agccgtccca taattaaacg
    11161 cgcgctggaa ggtggcataa atttctttga taccgccaat agctattccg atggtagcag
    11221 cgaagagatc gttggtcgcg cactgcggga tttcgcccgt cgtgaagacg tagtcgttgc
    11281 caccaaagtg ttccatcgcg ttggtgattt accggaagga ttatcccgtg cacaaatttt
    11341 gcgctctatc aacgacagcc tgcgacgtct cggcatggat tatgtcgata tcctgcaaat
    11401 tcatcgctgg gattacaaca cgccgatcga agagacgctg gaagccctga acgacgtggt
    11461 aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg cacgcttcgc agtttgctca
    11521 ggcgctggaa ctacaaaaac agcactgctg ggcgcagttt gtcagtatgc aggatcacta
    11581 taatctgatt tatcgcgaag aagagcgcga gatgctgcct ctgtgttatc aggagggcgt
    11641 ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg acgcgtccat ggggagaaac
    11701 taccgcacga ctggtgtctg atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa
    11761 tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa gaactcggtg caacacgagc
    11821 acaagttgcg ctggcctggt tgttaagtaa accgggcatt gccgcaccga ttatcggaac
    11881 ttcgcgggaa gaacagcttg atgagctgct gaacgcggtg gatatcactt tgaagccata
    11941 acagattgcc gagctggaaa cgccgtataa accgcatgcg gtcgtaggat ttaaataaga
    12001 gtccaggcct gatgagacgt gacaagcgtc acatcaggca tcggtgcaca actacgacag
    12061 aatacccagc ggccagtgat gaccgataaa gtacaggatg cctgcggaaa tcaccccggc
    12121 gacaatatcg tcgatcatga tccccatgcc gccatgtaca ttgcgatcaa accagcggat
    12181 cggccacggc ttccacatat ccagaatacg gaaaatcaca aacccggcgg caacccactg
    12241 ccagtcattg gtcggcagcg ccatgagcgt gatccacata ccaataaatt cgtcccagac
    12301 aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt tgatgacaaa gatagacgcc
    12361 gatacagatc cccaacatca ccaccagcga gtagagctgc cagggtaaaa aggtcatcag
    12421 ataccagaaa gggatcgccg ccagcgatcc catcgtgcca ggaacgattg ggcttaatcc
    12481 acttccgaat ccgacagcaa gcagatgcca cggattactc atcttcaggc gacttttcgc
    12541 gacatcttta tggcgtgaca aaatggtcat atcctttcca gtcaaacgtg acaggttcgc
    12601 cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat ttgcccgata caggtaaacg
    12661 gtacgccaag gtgtccgaga gcaacatcca gcgcgccacg gttcagttcc ggcaccgtga
    12721 aacacaactc gtaatcttca ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa
    12781 catggcgaga aagcgcatca gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt
    12841 cgctggcttt cacgatatgc ccgagatcgg aaatcagacc gtcagagaga tcgatagctg
    12901 aatttgccag atcgcgcagt gcctgcccct gtaaaatacg cggcgatgga cggagatgac
    12961 gtttgatcaa gtagtcagca tctttagcat cggcaacctg caaacggttt tgcaaaatcg
    13021 ccagcccggc ggcgctatcg cccggtgtac cggtcacata gatccagtca cccggtttcg
    13081 ccccagagcg cgttaaggct cgtcccatcg gaacaaagcc gtggataccc aacgtcattg
    13141 ataatggccc acgcgtggta tcgccgccaa tgagttgcat atcgtaataa ttgagaagat
    13201 caaacaaact gtcgctgaag gactcaagcc acgcttcgtc tacgtccggt aaggttaatg
    13261 ccagcgtcag ccaggccgga tcggccccca tcgctgccag atcgcttagg ttcaccgcca
    13321 gtgctttata agccagatca gcaggatcga tatcagggag gaaatggttt cccgccacca
    13381 gcgtatcagt gctgatcgcc agggtctgtt tctcggggat attgagaaga gcgcaatcgt
    13441 cgccgatgcc cagttcgaca tcaagacgag aacttcttac acggtcaaaa taacgggcaa
    13501 tcagggagaa ctcgccacat gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg
    13561 gcgatcatgg aacagtcttc cgtgaatcta ccggcctgga tattactttt tgttagggcg
    13621 aatcacaggt gctgctttat cgagtacgcc gttgacgaac ttatggctgt cttctgcgcc
    13681 gaacgatttc gccagttcga tcgcttcgtt aatggccact ttgtatggca catcactacg
    13741 tttagacagt tcgtacagcg caatgcgcag tactgctttt tctacctggc ccagttcttc
    13801 cagcaggcgg gacaggtagg gcttcatcag tccgtcgagg tatgcggtgt tagtcgccac
    13861 cccggccagc agctcacgga agtacaggac gtcaacgtct tttacatcct gttcagccag
    13921 gaactggtat tcaacatcag cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg
    13981 gacggcacac tcacgagcgc ggcgacgagc agcaggtttc acggatttcc ccttactaat
    14041 ttcaggcctt gatggctttc aatacattaa tcatttcaag cgcggtcagt gcagcttctg
    14101 cacctttgtt gccagctttg gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg
    14161 tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc
    14221 ttgcaccacc agcgacatat tcaaagtggg cagtgccacc acgaataacc gtacccagcg
    14281 caatcaccgc gtcgtattta ccggttttag ccagtgcacc cgccgccagc ggcagctcat
    14341 aggcaccagg cacccaaaca acggtaatgt tttcatcttt tacctgaccg atacgtttca
    14401 gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg
    14461 tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc aataatgttc attttcttcc
    14521 ttcgggttcg agtatggccc cgcagggggg cggattttag cataatattt cgtgcgctgc
    14581 ttccctttcg agccgggaga tcatgcaccc actaaatgca ggcaaacatc cgggcctaca
    14641 tgacgtatct ctttgaattt aaattggggg gcgtcggcta atttctcaag ccctggcagc
    14701 gagcataatc cacgggcgtc gctgcctaat agtttaggtg cgatatagac aatcagctca
    14761 tcgactaaac ccgcctgtag caatacgcca gcgagcgttg gccccgcttc cacccagatg
    14821 ctgttaatct gctgtttacc cagttgcatc atcagtacaa ccagatccag atgaccttta
    14881 tgctctggaa tcagcaaggt acgcaccgtt tccggccact cacgagaatc ttcctgcgta
    14941 cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat gttccggcgt cacgcgattt
    15001 tggctatcaa tcacaatacg tactggctgg cggagatttt gttgcgggta gagcgcctga
    15061 gtttgttcat ccagttcaga ccaacgcact gttaaggctg gatcatccgc cagcaccgtg
    15121 gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc gttgtacgtc gcgccgcgcc
    15181 tgaggcgaag tgatccactg gctttcgccg ctcgccatcg ccgtgcggcc atcaagcgat
    15241 gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca tccgcttgag aaagccttta
    15301 ttcaattgct cggcttcact catcatcagc ccgtggctga cgtcaatgcc agcctgttgc
    15361 agacggtaaa gtccacgccc agcgacctgc gggttaggat cttgcatcgc tgcgaccacg
    15421 cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg gcgtacggcc atgatggcta
    15481 cagggttcga gtgtcacata cgccgttgcg cctttggctt tttcacccgc catacgcaac
    15541 gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt aaccttcacc gacaatttcg
    15601 ccatctttga caatgacgca cccgacattc gggttggggt gggtggtaaa acgtcctcgt
    15661 tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct gcacggctta gtcctccagg
    15721 cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac tgcggtagac agaggcaaaa
    15781 cggatatagg cgactttatc gagctttttc aattgctcca tcaccagatt gccaatcatc
    15841 ttgctcggta cttcgcgctc accggtggcg cgcagctgcg atttaatatg attgattgcc
    15901 atttcaacgt aatcggaact caccggacgt ttttccagcg cccgcagcat tccgctacgc
    15961 aatttctctt cattaaacgg ttcacgcacg tcgttgcttt ttacaacacg cggcataacc
    16021 agctccgcca cttcaaaggt ggtgaaacgt tcattacaca ccagacactg ccgacggcgg
    16081 cgtacggatg aaccctcgcc cacgagacga gagtcaatta ccttagtgtc cacggcgaaa
    16141 cagaatgggc aatgcatacg gcgtcctgac caggtggtta acggaaatct attttaccct
    16201 gaactgacga tacaacaaag gcgcaacgct tttgagataa aggatcgatg ctttcgctgc
    16261 ttttaccctc taccattagg ctaattgcga cgagaaaggg acgacctatg aatactaacg
    16321 tttttcgact gctcctgctg ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg
    16381 aagtgcgaca gatgaaacac agcgtcagca cgctgaacca ggagatgacg caactcaatc
    16441 aagaaacagt caaaatcact cagcaaaaca ggctgaatgc aaaatccagc agtggggttt
    16501 accttctgcc tggagcgaaa acaccggcaa gactggaaag ccagatcggt actttacgta
    16561 tgtcgctggt gaatattacg cctgatacag atggcaccac ccttacacta cgtattcagg
    16621 gtgagtcgaa tgatcccttg cccgcattca gcggaactgt tgaatatggg caaattcagg
    16681 gaacaataga caattttcag gaaatcaatg tgcagaatca attgattaat gcccctgcca
    16741 gcgtcctcgc ccccagcgat gttgatattc cgttacaatt aaaggggatt tctgtggatc
    16801 agttaggctt tgtacgtatc cacgacattc aacctgttat gcagtaaacg tatttcggga
    16861 cgattttgtg cgtcccgcaa catcttcccc gtcattttgt tactctgctt acatcacctg
    16921 gattgatagt aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt
    16981 gtaaacgtga acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg
    17041 tgagcaatga tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc
    17101 gcctttttca ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac
    17161 tggcagccgg tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg
    17221 acaaaccgca gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc
    17281 acacccgttt cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta
    17341 aaaaagactg gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact
    17401 ccgatgctaa aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt
    17461 tctccatcga caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact
    17521 tcgcgaacaa ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt
    17581 acatgggtct gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct
    17641 atgcgaaata ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc
    17701 gtttcaaaat taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca
    17761 tcggcttcac caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca
    17821 acggtattaa gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact
    17881 acgatcactg gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg
    17941 acgatgcaga actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg
    18001 gttacctggt agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat
    18061 ttctgattgt taacaacgtg tgctttgttc atgccggatg cggcgtaaac gctttgtccg
    18121 gcccacaaaa gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag
    18181 cgcatcaggc aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag
    18241 cgaatgggtt gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg
    18301 tcgtaccata ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac
    18361 acaacaattc ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt
    18421 gaacggtaag ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc
    18481 acggatagat ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg
    18541 ggatgatagc catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga
    18601 tgcggggatt ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt
    18661 tcaacatcaa cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc
    18721 cactttctgc tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc
    18781 tacgtagatg gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc
    18841 ttccagtacc ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga
    18901 tgtgatcaag gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt
    18961 accgcgacgg atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt
    19021 aagcgagtaa ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg
    19081 gaataacgac aaaataccca gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc
    19141 tgccgccagt cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc
    19201 catcaacgcc atcgcaccgg tttgcgcaag gtcagccccc acgctcggac cgacgaactc
    19261 aatacgcttc actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct
    19321 gcccagcacc tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg
    19381 gctgctacca aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg
    19441 cattacgtca atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa
    19501 atccagcccc cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag
    19561 accagagatg ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg
    19621 gtttagttgt tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct
    19681 tgacgcgctt gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa
    19741 acatcgacgt cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac
    19801 ccactgcgta caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac
    19861 tgaatgcgcc acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact
    19921 cttctttaat acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga
    19981 cgatacccgc gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga
    20041 cgatcaagat caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga
    20101 agatgatcat gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct
    20161 caatgttctg catacccaag gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg
    20221 cgatcaacgc accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga
    20281 tgccggtgat acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct
    20341 cttcctgttt caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact
    20401 ccacaaacag ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt
    20461 tgccaccagc gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg
    20521 aagtggagtc ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct
    20581 gaccttcgcg ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg
    20641 cctgatcaac attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct
    20701 ctttcgcacg cgcagtgtcc tgaataccgg gcagttcaac aacgatacgg tcagcaccct
    20761 gacgctgaac caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa
    20821 tgttctgctg caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac
    20881 gcagctggtt gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg
    20941 caatggcttc atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt
    21001 ctttacgaac agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga
    21061 tattttgttc ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa
    21121 cgccgccacg caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc
    21181 gcggcgttgc cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta
    21241 atgcttcacg agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct
    21301 cttccagtgc cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga
    21361 tcagcgtttg ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct
    21421 caccaaacag gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga
    21481 cgtacttcca caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt
    21541 acagcgcctt catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta
    21601 cttcagtggt gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc
    21661 gaccaaccag gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct
    21721 ttttgtgttc tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac
    21781 cgaataccac cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg
    21841 ccgctaccgc atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca
    21901 acgttcaaag gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct
    21961 aatttaccct cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg
    22021 ttatgaatgg tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag
    22081 taagcgcgtg aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta
    22141 tcgctcttat atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca
    22201 tttcgggcgt tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct
    22261 tcaaccaggt cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt
    22321 tgcggacaca catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga
    22381 ccgccgacag cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt
    22441 aaatcttcgt aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg
    22501 tcaaaacgct cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg
    22561 taatcccagt cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca
    22621 agatcgtact gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc
    22681 gggttacgga agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc
    22741 tggaagccgc cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcagatcg
    22801 ccgtgcagtt tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg
    22861 ataatttgcg cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag
    22921 gtgccaacag gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca
    22981 cggcgtgcgc gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag
    23041 aaaaacagtc caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt
    23101 gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct
    23161 tctaccgccg ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc
    23221 ataatcagcg tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac
    23281 tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt
    23341 tttgccgcct gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc
    23401 cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg
    23461 tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc
    23521 gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc
    23581 aaaggctcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta
    23641 taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc
    23701 agcggcatat ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg
    23761 acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc
    23821 agcaacagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt
    23881 ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccgctggc tttacgccca
    23941 aacagacgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc
    24001 ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac
    24061 agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat
    24121 tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag
    24181 tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat
    24241 gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt
    24301 actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg
    24361 cattcatatg catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacaggagt
    24421 ggtttcgtag tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact
    24481 ttctttcccg ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttgtctgtt
    24541 atgcccgcga gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga
    24601 acaattactt gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga
    24661 acgtgttaaa cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt
    24721 acgatttaga cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga
    24781 tgatggcgca ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga
    24841 aatggcaggc aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca
    24901 ttgcacggca ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt
    24961 gattgccacc agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc
    25021 cgcatacagc acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat
    25081 cattcgccgg tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg
    25141 tttacggcaa aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca
    25201 cggtacacca ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg
    25261 accgagcagc gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa
    25321 ttgttgttga tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc
    25381 gtcaatttgc tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa
    25441 tgtgaagcca cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc
    25501 atcgccaaaa tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt
    25561 gatcccggca acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac
    25621 cacatccagc cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact
    25681 gtcttccccg cgataaattt cattcaccag actttccgac tgataatcca gcttcgtcaa
    25741 gctggcatag ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg
    25801 ccagggcgat tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca
    25861 ggcatgggaa tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg
    25921 ctgcgtattg tgtcgcaaac gcagcaacac cccatcaccg ccaaactgcg gatcgacatg
    25981 gcgataatcc tcggtatcgt atttatgtac gctgggagct ttaaacaccg gattgagata
    26041 cagcgctgtc acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc
    26101 gccgccatag aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa
    26161 gatgatctct tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg
    26221 aagactacgc gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg
    26281 tgggccgata tcaggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct
    26341 gaagccctgc ggtgtaaacc aacgctggcg atcgtgccac agcagtttga aactgtaacg
    26401 ccgccggggt tgtccgctgg agagatcaat cgccgcacgc catgcggtga cgcctggctg
    26461 cggctgactg cgctgcttat gcattggtac tgacatttct tcgttatcgt gttctgtacg
    26521 cagcataatg cgctgcggtg ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc
    26581 tttgctttgt ttaacaaatg ggggcaccgg caggtgccat gcatttaaca tcataattcc
    26641 cctgtgatga aaatctgatc accttgccat agcctaatgc aggatcactc atcatctgaa
    26701 ggatatttgg gggaggagga cgggggcgga taactttcgg aatagccgga tcaggatttc
    26761 ctcctgaacc ggcttacaga acgcaagtat tattggtttt cagccagctg acgatcgtgg
    26821 cggcgtttaa acatccagcc aatcaacagc acaacaatcc acgcgaaacc gacatacagc
    26881 gagatacgcg tatccgggtg ataaccaatc aacccgataa taaagagcag gaaaatcagc
    26941 ccgccgatgg tcgttgctac cccacccggc actttaaatt tcagcgcctt aacttcttct
    27001 ggcggcaaac ggcgacggaa ggcgatttgc gacagcagga tcataatcca cacccatacc
    27061 gtggcgaagg ttgccagcga agcgatcacc aggaagacgt tttccggcat gatgtagttc
    27121 agatacaccg caaacagcag cgcggtagtc atcaccagca ccgtaaccca cggaataccg
    27181 cgacgcgacg ttttgctgaa aattttcggt gcgctgccct gctctgccat accgtggagc
    27241 atacggccta cgccaaatac gtcactgtta attgccgaca gcgaagcggt cagcacaaca
    27301 aagttaagaa tgctggcggc aaaggtaatg cccatatgct ggaacgtcag tacgaacggg
    27361 ctaccggcag tgccaacctg attccacggg tagatagaca taatgacgaa cagcgtaccg
    27421 acgtagaaca ccagaatacg catcggcacg gagttaatcg cacgcggaat cgatttctca
    27481 ggatctttcg cttcaccggc ggtaatcccg ataatttcga tcccaccgta agcaaacatc
    27541 accatttgca acgacattac catgccaagc cagccgttac tgaagaagcc gccgttgctc
    27601 cacaggttat gaataccggt cggttgcccg ccgttgccaa tcccccagat gatgatgccg
    27661 aaaccggcga caatcatgat gatgatggtg gcgactttaa agaacgagaa ccagaattcc
    27721 agctcaccga agaccttcac gctcatcagg tttacggcgc aaatgatcag caccacgctc
    27781 agtacccaaa tccagtgcgg caccgtcggg aacaagacac ccatatagat accaaaagcg
    27841 gtcacatcgg cgatggcgac aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa
    27901 cctgccagcg ggccaaggtt ttcctgcgca taacgcgaga aagagctggc ggccgggtta
    27961 tgtaccgaca tttcccccag cgcacgcata atgatatacg ccgcgatacc accgataata
    28021 taggccaaca acacgctcgg accggccatt ttgatggcgt ctgccgaacc gtaaaacagc
    28081 ccggtgccaa ttgctgaacc cagtgccata aagcgtatgt ggcgggtact tagcccacgc
    28141 tttagcttat tcttactttc catcaattcc taacccatca acacaataaa aaaccacggg
    28201 gcatcgcagc cccgtggtta aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc
    28261 tgacgacctg ccgcgcgatc ccagataatg gccagaacca ccatcaccac tgttggcatt
    28321 aaccacgcca gaccttgttc ggccagcggt aaacgctgcg cccaggacgg taagatatcg
    28381 ctgaatgcag atgctttgat cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc
    28441 ggagcaatca cgcgggacga attatgccac catgagcgtg taaaacttaa tacaaccagt
    28501 gcgatacacg gcggataaat agcggtcagc accggtacgg agatctggat cagctggctt
    28561 aagccgaggt tagacaccac catcgagaag ccgccgagga taaacaccag cgtacgataa
    28621 gagagcggta cgtactgggc aaagaattct gcacaagcac aggtcaggcc aaccgccgtg
    28681 accagacagg cgatgaagat taacgccgcc agcaggaagc taccgccgcc gccaaaggtg
    28741 tgctgaacgt aagcatgcag aatagcagcg ccgtttgcag actgatcgac cagcgacgcg
    28801 ctgtctgaac ccagacagaa cagcgccagg tacagcaaag tcagaccaac acccgccatc
    28861 aggccagccc agacggtata acgggtcagc agacgcgctt cggtaacgcc acgagaacgc
    28921 gccgcgttaa caataacgat accaaacacc attgcgccca gcgtatccat ggtcagatag
    28981 ccgttaacga agccgttaga aaatgcagcg ttttgataag cctcagtcgc cgtgctgata
    29041 gaacccgccg gccagacaat cgcgacaaca gacaggatga ccagcgcgat aattttcagc
    29101 ggcgcaagga agttgcccac ggtatccagc agcttgcccg gatagagcga aaccagaata
    29161 acgatagcga aatagaccag gctgtagata aacagcggca gcgcggaatc acccgtcagc
    29221 ggcgcaatcc ccacttcaaa ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc
    29281 accgccaggt aacaaaccgt tgccagcagt acgccagcga ctttaccgat tggcgtgctg
    29341 aggctgtcaa caccgccgcc aacttttgcc agcgccacta ccgttaatac cggcaggcca
    29401 acggcagtaa tgaggaagcc gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg
    29461 accattggag ggaaaataat gttacctgcg ccgacgaaca acgcaaatgt cataaagccc
    29521 agagcgatga tatcgcgcga tcttaattga tgggtcataa atcatactgc ctgtggatgt
    29581 ggtgttgtaa cgttaaattt ttgtgccaat ccctggggat gatacagcgg tatatttaaa
    29641 caattttagc gggaaatact cccaaccaag cgcggcgaag aatagttttt cgatttaccg
    29701 cgttaatgca gtcagtatga cagcacctga agcgcaattt aaacgcttat aacgttttaa
    29761 agcaaggagg gatctaaaaa ccagaggtat atagcgataa gaaatcacca accagtataa
    29821 aacactattt ccgtgaaaaa gacatggcta atcatgcgaa caaaaaatca ccaaccgttg
    29881 taaaatctaa cgcccgtatt ttgactgcaa agaaaataag ccagcatcgg ctggcttatg
    29941 gcaataaaag atgacaaagg cggattaatc gctgtttttg gcaattaaac gttccgggat
    30001 aacaaaactg aaacgtgttc cttttcctac tgtactctca atattcaggc gactttcgtg
    30061 atgattcacc gcatgtttca cgatcgctaa ccctaatccg ctaccgccgg tttgccggga
    30121 acgcgcttta tcaacgcgat aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat
    30181 gcccggtccg ttatcttcaa cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac
    30241 ggtgatatgc gtgccttccg gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc
    30301 actgcgtagc tgatcttcgt tgccagacac cttgaggccg ttatctatct caaaggtaaa
    30361 tgtctgtttt ttctgactca gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg
    30421 cacatcaacc ttttcattga gcaattgcgt cggcgcggct tcaattttcg acagcgttag
    30481 caactgcttc accagccctt ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt
    30541 ttcgcgtacc gcgccttcca gcggctgctc atccatcatc tccaggtaac cctgtaacac
    30601 ggtcaatggc gtacgtaact catggctcac gttggcaaaa aagttacgcc gcgccccttc
    30661 cagttgatgc atttgcgtga catcacgcgc caccatcagc aactgtttgt gggtataagg
    30721 catgacgcga atttccagat gccgcccggt gttgagcacc agattgagcg ggcgagaaaa
    30781 atcacgcgtt ttcagatatt gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg
    30841 cccgttatct tccggccagc gcaaaccaag aatttgttgc gccagaccgt tacaccagaa
    30901 aataccgccc tcttccgtgg tcagcaccac cgcgtcgggc agcgactccg cgccgctacg
    30961 aaagcgttta atcagattgc ccagttcacg gcggcgtttt ttatttcgca gctgcatctg
    31021 gtgtaagccg tatagtagcg gttcccagct accacgcccc ggtggcgggg tcatactgcg
    31081 atccacccac agccaccatg aaaggcgcaa taaattccag aaatgccaga taagcagtcc
    31141 tgttaccgat gccagcaaaa accagggcag gtaaccaaaa aatgcaccca ggatgaaagc
    31201 cgggaggcag caaagtagca gctccagcac cagccttttc cacgacagcc gttccagcac
    31261 gtaagatact ccagttaaga aatcataagc cctgctctgc gtccgatgag caaggcgtta
    31321 aaagcgggtt gaaaaacgat atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc
    31381 gccgggctcc agtgctttac gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac
    31441 atacacgtta gttccccaga cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg
    31501 atgcgtcata aaaaagtgca gcagtttaaa ttctgtcggc cccatctcca gcggctcttc
    31561 gcccgccatc actcggtgag atgtcgggtc gagacttaat ccctgcatct taatcacctc
    31621 ttccaccgcc attggcgaaa tacggcgcat taccgctttg attcgcgcca ccagctcctt
    31681 cggcgaaaat ggcttggtga tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc
    31741 ttcttcttcc cctctggcgg tcaacatcac cactggaata tcccgggtca tcgactcgcg
    31801 cttgaggtgt ttgatgaact ggataccgga gccgccaggt aacatccagt caaggagaat
    31861 taaatccggc tagggttcat tcagttgatt cacagcactg tcataatctt ccgcttcgac
    31921 cggctgaaag ccattttgtt cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc
    31981 ttctacgacc agaatacgtc tcgccatgat ttgccctgtt gtaataaata ggttgcgatc
    32041 attaatgcga cgtcattatg cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca
    32101 tatcttcagg ttattgattt ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt
    32161 ataatcgcga ccatgctttt tcgccaggga accgttatgc gcatccttca cacctcagac
    32221 tggcatctcg gccagaactt ctacagtaaa agccgcgaag ctgaacatca ggcttttctt
    32281 gactggctgc tggagacagc acaaacccat cagatggatg cgattattgt tgctggtgat
    32341 gttttcgata ccggctcgcc gcccagttac gcccgcacgt tatacaaccg ttttgttgtc
    32401 aatttacagc aaactggctg tcatctggtg gtactggcag gaaaccatga ctcggtcgcc
    32461 acgctgaatg aatcgcgcga tatcatggcg ttcctcaata caaccgtggt cgccagcgcc
    32521 ggacatgcgc cgcaaatctt gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc
    32581 attccgtttt tacgcccgcg tgacattatt tccagccagg cggggcttaa cggtattgaa
    32641 aaacagcagc atttactggc agcgattacc gattattacc aacaacacta tgccgatgcc
    32701 tgcaaactgc gcggcgatca gcctctgccc atcatcgcca cgggacattt aacgaccgtg
    32761 ggttccagta aaagtgacgc cgtgcgtgac atttatattg gcacgctgga cgcgtttccg
    32821 gcacaaaact ttccaccagc cgactacatc gcgctcgggc atattcaccg cgcacagatt
    32881 attggcggca tggaacatgt tcgctattgc ggttccccca ttccactgag ttttgatgaa
    32941 tgcggtaaga gtaaatatgt ccatctggtg acattttcaa acggcaaatt agagagcgtg
    33001 gaaaacctga acgtaccggt aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg
    33061 attaccgcac agctggaaca gtggcgcgat gtatcgcagg agccacctgt ctggctggat
    33121 atcgaaatca ctactgatga gtatctgcat gatattcagc gcaaaatcca ggcattaacc
    33181 gaatcattgc ctgtcgaagt attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg
    33241 ttagccagcc aacagcgtga aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc
    33301 cgtctggcac tggaagaact ggatgaatcg cagcagcaac gtctgcagca tcttttcacc
    33361 acgacgttgc gtaccctcgc cggagaacac gaagcatgaa aattctcagc ctgcgcctga
    33421 aaaacctgaa ctcattaaaa ggcgaatgga agattgattt cacccgcgag ccgttcgcca
    33481 gcaacgggct gtttgccatt accggcccaa ccggtgcggg gaaaaccacc ctgctggacg
    33541 ccatttgtct ggcgctgtat cacgaaactc cgcgtctctc taacgtttca caatcgcaaa
    33601 atgatctcat gacccgcgat accgctgaat gtctggcgga ggtggagttt gaagtgaaag
    33661 gtgaagcgta tcgcgcgttc tggagccaga atcgggcgcg taaccagccg gacggtaatt
    33721 tgcaggtgcc tcgcgtagag ctggcgcgct gcgccgacgg caaaattctc gccgacaaag
    33781 tgaaagataa gctggaactg acagcgacgt taaccgggct ggattacggg cgcttcaccc
    33841 gttcgatgct gctttcgcag gggcaatttg ctgccttcct gaatgccaaa cccaaagaac
    33901 gcgcggaatt gctcgaggag ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg
    33961 tttttgagca gcacaaatcg gcccgcactg agctggagaa gctgcaagcg caggccagcg
    34021 gcgtcacgtt gctcacgccg gaacaagtgc aatcgctgac agcgagtttg caggtactta
    34081 ctgacgaaga aaaacagtta attaccgcgc agcagcaaga acaacaatcg ctaaactggt
    34141 taacgcgtca ggacgaattg cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg
    34201 cgttagccga agaagaaaaa gcgcaacctc aactggcggc gcttagtctg gcacaaccgg
    34261 cacgaaatct tcgtccacac tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata
    34321 ttcgccagca gattgaagaa gtaaatactc gcttacagag cacaatggcg cttcgcgcga
    34381 gcattcgcca ccacgcggcg aagcagtcag cagaattaca gcagcagcaa caaagcctga
    34441 atacctggtt acaggaacac gaccgcttcc gtcagtggaa caacgaactg gcgggttggc
    34501 gtgcgcagtt ctcccaacaa accagcgatc gcgagcatct gcggcaatgg cagcaacagt
    34561 taacccatgc tgagcaaaaa cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg
    34621 atgaagttgc taccgccctg gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg
    34681 tcgcgctgca tgggcagatt gttccccaac aaaaacgtct ggcgcagtta atcgtcacta
    34741 tccagaatgt cactctggaa cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc
    34801 gttataaaga aaagacgcag caacttgccg atgtgaaaac catttgcgag caggaagcgc
    34861 gcatcaaaac gctggaagcc cagcgtgcac agttacaggc gggtcagcct tgtccacttt
    34921 gtggttccac cagccacccg gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc
    34981 agtctcgatt actggcgctg gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc
    35041 tgcgcgggca actggatgca ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa
    35101 gcctccgaca agatgagcaa gcacttactc aacaatggca agccgtcacg gccagcctca
    35161 atatcacctt gcagccacag gacgatattc aaccgtggct ggatgcacaa gatgagcacg
    35221 aacgccagct gcggttactc agccaacgac atgaattaca agggcagatt gccgcgcata
    35281 atcagcaaat tatccagtat caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg
    35341 cattggcggg ttatgcactg acattgccac aggaagatga agaagagagc tggttggcga
    35401 cacgtcagca agaagcgcag agctggcagc aacgccagaa cgaattaacc gcgctgcaaa
    35461 accgtattca gcagctgaca ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc
    35521 acagcgaaga aactgtggcg ctggataact ggcggcaggt tcatgaacaa tgtctcgcat
    35581 tacacagcca gcagcagacg ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa
    35641 aagcccaagc gcagtttgac accgcgctac aggccagcgt ctttgacgat cagcaggcgt
    35701 tccttgcggc gctaatggat gaacaaacac taacgcagct ggaacagctc aagcagaatc
    35761 tggaaaacca gcgccgtcag gcgcaaacgc tggtcactca gacagcagaa acgctggcac
    35821 agcatcaaca acaccgacct gacgggttgg ctctcactgt gacggtggag cagattcagc
    35881 aagagttagc gcaaactcac caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc
    35941 gccagcagct gaagcaggat gcagataacc gtcagcaaca acaaacctta atgcagcaaa
    36001 ttgctcaaat gacgcagcag gttgaggact ggggatatct gaattcgcta ataggttcca
    36061 aagagggcga taaattccgc aagtttgccc aggggctgac gctggataat ttagtccatc
    36121 tcgctaatca gcaacttacc cggctgcacg ggcgctatct gttacagcgc aaagccagcg
    36181 aggcgctgga agtcgaggtt gttgatacct ggcaggcaga tgcggtacgc gatacccgta
    36241 ccctttccgg cggcgaaagt ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc
    36301 tggtcagcca taaaacacgt attgactcgc tgttccttga tgaaggtttt ggcacgctgg
    36361 atagcgaaac gctggatacc gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa
    36421 ccatcggtgt gattagccac gtagaagcga tgaaagagcg tattccggtg cagatcaaag
    36481 tgaaaaagat caacggcctg ggctacagca aactggaaag tacgtttgca gtgaaataac
    36541 tattcagcag gataatgaat acagaggggt gaattatctc ttggccttgc tggtcgttat
    36601 cctgcaagct atcactttat tggctacggt gattggtagc cgttctggtg gttgtgatgg
    36661 tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg ggatggccga
    36721 atttggcatt atgagcgtgc tcacggagct ggcgcataac gtaggaattt cgattcctgc
    36781 tgccgggcat atgatctcgt attatgcact ggtggtggtg gtcggtgcgc caatcatcgc
    36841 actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt
    36901 cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca ttggtcggct
    36961 ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat
    37021 tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga tgacagtcgc
    37081 caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatgctggc gttacacctt
    37141 tttattgatc gctgttttta atattgcggt gatggcatcg gtctattttt gggtgccaga
    37201 tattcgcgac gaggcgaaag gaaatctgcg cgaacaattt cactttttgc gcagcccggc
    37261 cccgtggtta attttcgccg ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag
    37321 ctacgtaaag ccatacatga tgtttatttc cggtttttcg gaaacggcga tgacctttat
    37381 tatgatgtta gttgggctag ggatggtgct gggaaatatg ctaagtggca ggatttcagg
    37441 acgttattca ccactgcgca ttgcagcagt gactgacttt atcattgtac tggcactgct
    37501 gatgctcttt ttctgcggcg gcatgaaaac aacgtcgctt atttttgctt ttatttgttg
    37561 cgcgggatta tttgcccttt cagcacctct gcaaatattg ttactgcaaa atgccaaagg
    37621 aggagagtta ttaggtggcg caggtgggca aatagcgttt aacctcggta gcgccgtcgg
    37681 cgcatattgc ggaggtatga tgctgacgct ggggctggca tataattacg tggcgctgcc
    37741 tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg
    37801 ccagcaagcg gcggatagtc cggtgctggc gaaaccactg gggtagatta ctgtcacggt
    37861 gcgcgttgct tattgccgga tgcggcgtga acgccttatc cgccctacgc ggttctggca
    37921 cattttgtag gcctgataag acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc
    37981 tacgtttttt actcttgtgg ccataaccac gcagcgccgc gtacgccgct ggaatcaccg
    38041 tgcttcgcct tactcaccgg cgtttcacat tcgccgccga agacaaattg tttaatcaac
    38101 tgcccaaccg tttgatataa acggtctaca ttgctcatcc cgccccccag gacaatcaca
    38161 tccggatcga gaatattcac gacatgtgcc agcgattttg ccagccgcag ctcgtagcga
    38221 cgcaatgcca gttccgctac cggatcgctt tcttcaacca ggcggataat ttcactgcct
    38281 ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga atcccgtgcc cgaaataaag
    38341 gtttcaatac aaccttgttt accgcaataa caagggactt cctcgcgata acgcagttcg
    38401 tcttcgtcca tccacggtag cggattgtgt ccccactcac ctgccgtgcc attgccgccg
    38461 atatgcgtcc gcccattgaa tgccacgccc gcgccgcatc ccgtgccgat agtcacggca
    38521 aataccgtct gcgctcccgc tgccgcgcca tctactgctt ctgaaaccgc cagacagttg
    38581 gcatcatttg ccagccgcac ttcccgctgc aacctcgcgc ttaagtcttt atcgaatggc
    38641 tgaccgttga gccaggttga attgacattc ttcaccacac cggtgtaggg cgaaatcgaa
    38701 cctggaatgc ccatacctac cgttccgcgc tgccctgtcg cctgctccgc catatcaacc
    38761 aacgtggcga tcgtttcaat agtctgccgg taatcatcac gcggcgtggg cagacgatgg
    38821 cggtacaact gctcccctgc atcgcccagt gcaatcactt cagttttggt gccgcctaaa
    38881 tcgataccta tacgcactgt actctcctta tttttttcaa tatcaatagc gtagagacgg
    38941 acaaccggat tggcaatgca aggccgccga caattcgtta tcatgcccgc taaatttaac
    39001 gacaaggccg tggaaattat catgctgtgg ttcaaaaatt taatggttta ccgtcttagc
    39061 cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc tagcctcgat ggcatttacc
    39121 ccatgcggca gccaggacat ggcgaagatg ggctgggttc ctccgatggg atcgcacagc
    39181 gatgcgttaa cgcacgttgc caatggtcaa attgttatct gcgcgcgcaa agaagaaaaa
    39241 atcctcccgt ctccggtgat taaacaggcg ctggaagcga aaatcgccaa actggaagcg
    39301 gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc tgaaagacga agtgctgcac
    39361 tctctgctgc cgcgtgcttt cagccgtttt agccagacaa tgatgtggct cgacacggtt
    39421 aacggtttga ttatggtgga ctgcgccagt gccaaaaaag cggaagatac gctggcatta
    39481 ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga gcatggaaaa cccgattgaa
    39541 ctgacgctga ccgaatgggt tcgctccggt agtgcggcac agggcttcca gctgcttgat
    39601 gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga tccgcgcgaa gaaacaagat
    39661 ctgaccagcg aagagatcac caatcacatt gaagccggaa aagtggtgac taaactggcg
    39721 ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg atggttcgct caagcgtctt
    39781 aagttctgcg acgagctgcg cgatcaaaac gaagatatcg accgtgaaga tttcgcccag
    39841 cgttttgatg ccgatttcat cctgatgact ggtgaactgg cagcgttaat tcaaaacctg
    39901 attgaaggat taggtggcga agcacaacgt taattgctga ttttccttta atgccggatg
    39961 cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc aagcatataa ttattcgcta
    40021 cggcgagcaa taatttttag cgcagcaata ttatgcgttt tacgccgtaa ctgatctaat
    40081 cgcctcatcc ctattcgcct gtcagcagca cgcagaatat tcactaaccc ccatttatcg
    40141 ctggtaaatt gttttgttaa cagctccgct tttggggtat caatatattc acgcaataaa
    40201 tcagaaattg aacaaatatc cgtcaggtaa ggataccagc aagtttcacc gtgaaaatta
    40261 cgcactccag cgttacctgc aataaagtct ttcgggtaat cccagatcac gtttttatcc
    40321 aacgtgatcc aataacgcgg taaatcggta cttccaccat tttggctgcg catgggataa
    40381 cgggtacagt gaatttgcag gttgatcgtt ggcgtcagta agtcatagat ctcccgctgt
    40441 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
    40501 gaattttccg cgttgccccg gttttcacta aatcacgtaa ctgcgaaaga tcgggttatt
    40561 cgacaaataa gagtaatgat ggatcctctt ttaaccacgc cgttttcaca tcggcagcca
    40621 gctgtggatt attaatagcc agcgaccgat cctgaggctc agtcaatgtc gttaacaacg
    40681 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
    40741 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaaaaa
    40801 atacctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    40861 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    40921 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggta
    40981 ggttcggcaa cttgaagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    41041 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    41101 gtcatctctt ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg
    41161 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    41221 tcattacttt gaagcataaa ctggccctgt ctggtagttg aacatctact tcactatagg
    41281 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    41341 tcaatacgtt agagagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac
    41401 gttggtgaaa tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt
    41461 tcccagcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    41521 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    41581 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    41641 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    41701 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc agatctcgct
    41761 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    41821 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    41881 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    41941 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    42001 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    42061 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    42121 tgattccagt aatggaatgt tattgtttaa tattctaatt attagaaaaa cataaattat
    42181 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    42241 cggtcactat ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    42301 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    42361 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    42421 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    42481 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    42541 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    42601 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt
    42661 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    42721 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    42781 tagcgataac ggtggatgcc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa
    42841 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    42901 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    42961 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    43021 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    43081 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtaaaaatct caatacatcc
    43141 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    43201 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    43261 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    43321 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcgaatt
    43381 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    43441 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    43501 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    43561 taccagcggc atctgcatac gttccgccgc gagatacaaa atctctttaa ttacattggg
    43621 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttaggt aatcctctat
    43681 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    43741 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    43801 gcaatatggg aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc
    43861 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    43921 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    43981 ttaaacctgg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    44041 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    44101 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    44161 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    44221 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    44281 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    44341 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    44401 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    44461 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    44521 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    44581 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    44641 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    44701 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    44761 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    44821 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    44881 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    44941 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    45001 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    45061 gcccgtcatg ccgtcgcgag tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    45121 ggttgccgtc tggtagctga cccagccaaa catcagagga taaacgacaa taatgggaag
    45181 ggagagccac cattccagcg gagcactatt gaacgacacg gtaatgcccg taagctcgag
    45241 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    45301 tgcccccatc aaattcagac acagaatcat caacatagcg gtggaaggca gcacgtttac
    45361 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    45421 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    45481 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    45541 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    45601 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    45661 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca
    45721 attttcccaa attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac
    45781 tttcgtagct cacccgttag cacta
//
LOCUS       DAECNO010000024        45313 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.024, whole genome shotgun sequence.
ACCESSION   DAECNO010000024 DAECNO010000000
VERSION     DAECNO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.024"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            133..576
                     /locus_tag="KKO08_001329"
     CDS             133..576
                     /locus_tag="KKO08_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6317647.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="KKO08_001330"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="KKO08_001330"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBD6317648.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="KKO08_001331"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="KKO08_001331"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBD6317649.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="KKO08_001332"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="KKO08_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBD6317650.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="KKO08_001333"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="KKO08_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6317651.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="KKO08_001334"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="KKO08_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBD6317652.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="KKO08_001335"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="KKO08_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBD6317653.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="KKO08_001336"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="KKO08_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBD6317654.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="KKO08_001337"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="KKO08_001337"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBD6317655.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="KKO08_001338"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="KKO08_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBD6317656.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="KKO08_001339"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="KKO08_001339"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBD6317657.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="KKO08_001340"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="KKO08_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBD6317658.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="KKO08_001341"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="KKO08_001341"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBD6317659.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="KKO08_001342"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="KKO08_001342"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBD6317660.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="KKO08_001343"
     CDS             13936..14316
                     /locus_tag="KKO08_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBD6317661.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="KKO08_001344"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="KKO08_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBD6317662.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="KKO08_001345"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="KKO08_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBD6317663.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="KKO08_001346"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="KKO08_001346"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBD6317664.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="KKO08_001347"
     tRNA            complement(17417..17492)
                     /locus_tag="KKO08_001347"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="KKO08_001348"
     tRNA            complement(17496..17571)
                     /locus_tag="KKO08_001348"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="KKO08_001349"
     tRNA            complement(17623..17698)
                     /locus_tag="KKO08_001349"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="KKO08_001350"
     tRNA            complement(17701..17776)
                     /locus_tag="KKO08_001350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17913..17988)
                     /locus_tag="KKO08_001351"
     tRNA            complement(17913..17988)
                     /locus_tag="KKO08_001351"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17953..17955),aa:Lys,seq:ttt)
     gene            complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="KKO08_001352"
     CDS             complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="KKO08_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBD6317665.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18954..19475)
                     /gene="pal"
                     /locus_tag="KKO08_001353"
     CDS             complement(18954..19475)
                     /gene="pal"
                     /locus_tag="KKO08_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBD6317666.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="KKO08_001354"
     CDS             complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="KKO08_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBD6317667.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20935..22176)
                     /gene="tolA"
                     /locus_tag="KKO08_001355"
     CDS             complement(20935..22176)
                     /gene="tolA"
                     /locus_tag="KKO08_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBD6317668.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22241..22669)
                     /gene="tolR"
                     /locus_tag="KKO08_001356"
     CDS             complement(22241..22669)
                     /gene="tolR"
                     /locus_tag="KKO08_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBD6317669.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22673..23365)
                     /gene="tolQ"
                     /locus_tag="KKO08_001357"
     CDS             complement(22673..23365)
                     /gene="tolQ"
                     /locus_tag="KKO08_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBD6317670.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23362..23766)
                     /gene="ybgC"
                     /locus_tag="KKO08_001358"
     CDS             complement(23362..23766)
                     /gene="ybgC"
                     /locus_tag="KKO08_001358"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBD6317671.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23916..24209)
                     /gene="ybgE"
                     /locus_tag="KKO08_001359"
     CDS             complement(23916..24209)
                     /gene="ybgE"
                     /locus_tag="KKO08_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBD6317672.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24209..24322)
                     /gene="cydX"
                     /locus_tag="KKO08_001360"
     CDS             complement(24209..24322)
                     /gene="cydX"
                     /locus_tag="KKO08_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBD6317673.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24337..25476)
                     /gene="cydB"
                     /locus_tag="KKO08_001361"
     CDS             complement(24337..25476)
                     /gene="cydB"
                     /locus_tag="KKO08_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD6317674.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25492..27060)
                     /gene="cydA"
                     /locus_tag="KKO08_001362"
     CDS             complement(25492..27060)
                     /gene="cydA"
                     /locus_tag="KKO08_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBD6317675.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27909..30542)
                     /gene="mngB"
                     /locus_tag="KKO08_001363"
                     /pseudo
     CDS             complement(27909..30542)
                     /gene="mngB"
                     /locus_tag="KKO08_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(30560..32476)
                     /gene="mngA"
                     /locus_tag="KKO08_001364"
     CDS             complement(30560..32476)
                     /gene="mngA"
                     /locus_tag="KKO08_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBD6317676.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32646..33368
                     /gene="mngR"
                     /locus_tag="KKO08_001365"
     CDS             32646..33368
                     /gene="mngR"
                     /locus_tag="KKO08_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBD6317677.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33471..34340)
                     /gene="sucD"
                     /locus_tag="KKO08_001366"
     CDS             complement(33471..34340)
                     /gene="sucD"
                     /locus_tag="KKO08_001366"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBD6317678.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34340..35506)
                     /gene="sucC"
                     /locus_tag="KKO08_001367"
     CDS             complement(34340..35506)
                     /gene="sucC"
                     /locus_tag="KKO08_001367"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBD6317679.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35600..36817)
                     /gene="odhB"
                     /locus_tag="KKO08_001368"
     CDS             complement(35600..36817)
                     /gene="odhB"
                     /locus_tag="KKO08_001368"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBD6317680.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36832..39633)
                     /gene="sucA"
                     /locus_tag="KKO08_001369"
     CDS             complement(36832..39633)
                     /gene="sucA"
                     /locus_tag="KKO08_001369"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBD6317681.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39934..40650)
                     /gene="sdhB"
                     /locus_tag="KKO08_001370"
     CDS             complement(39934..40650)
                     /gene="sdhB"
                     /locus_tag="KKO08_001370"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBD6317682.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40666..42432)
                     /gene="sdhA"
                     /locus_tag="KKO08_001371"
     CDS             complement(40666..42432)
                     /gene="sdhA"
                     /locus_tag="KKO08_001371"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBD6317683.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42432..42779)
                     /gene="sdhD"
                     /locus_tag="KKO08_001372"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42432..42779)
                     /gene="sdhD"
                     /locus_tag="KKO08_001372"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBD6317684.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42773..43177)
                     /gene="sdhC"
                     /locus_tag="KKO08_001373"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42773..43177)
                     /gene="sdhC"
                     /locus_tag="KKO08_001373"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD6317685.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43558..43665
                     /locus_tag="KKO08_001374"
     CDS             43558..43665
                     /locus_tag="KKO08_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317686.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43871..45154
                     /gene="gltA"
                     /locus_tag="KKO08_001375"
     CDS             43871..45154
                     /gene="gltA"
                     /locus_tag="KKO08_001375"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBD6317687.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT        11181 a        12087 c        11136 g        10909 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccacaa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatct cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcta tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag caaacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatat taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtggtgggtc
    17821 gtgcaggatg actcgacttc gtctcgccct tcggcgccgt tgctgatgca acgttatcct
    17881 tcacgtttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat tcgaacctgc
    17941 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
    18001 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
    18061 gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag
    18121 aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg tttttgtgcc
    18181 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
    18241 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc
    18301 tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt
    18361 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
    18421 tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta
    18481 tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc
    18541 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgt cgcaccgctt
    18601 tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac
    18661 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
    18721 ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa
    18781 agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct
    18841 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
    18901 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
    18961 accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag tactgcaggt
    19021 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
    19081 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
    19141 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
    19201 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
    19261 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
    19321 atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat gccttcgctg
    19381 ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca
    19441 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
    19501 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
    19561 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
    19621 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
    19681 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
    19741 cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
    19801 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
    19861 aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat ccgggaacca
    19921 ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa
    19981 atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca gtttgctgcc
    20041 gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc
    20101 gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg tcacataagc
    20161 cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg aacggtgaac
    20221 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
    20281 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt
    20341 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
    20401 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
    20461 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatgcc
    20521 cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
    20581 agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa cgatgccgcc
    20641 aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac caataggacg
    20701 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
    20761 cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg
    20821 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
    20881 aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc gcgattacgg
    20941 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
    21001 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
    21061 aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt
    21121 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
    21181 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg
    21241 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
    21301 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
    21361 tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
    21421 agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
    21481 tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct cagcttctgc
    21541 cttggctttt tcagcagctt cagttgctgc tttctttctt gcttcagctg cagctgcttc
    21601 tgccgcttcc gctttcttct tcagtgccgc agcggctacc tcggcttttt tctgcgcttc
    21661 ggctgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt
    21721 cgctgcttct tccgcagctt tagcatctgc cgccgctttc gctgccgcca cttcagcttg
    21781 cttctgcttt aactcggcct gttttgcggc ttcttcagcc tgctttttct gctcctgagc
    21841 cgctaaccgc tctttctcaa gttgcttcag gcgttcctgt tcagccgctt gtttctcacg
    21901 cagttcttca gcagcctgct gttccttcat cttgcgctgt tcatcagaac gcttcgcgct
    21961 tgattcctgg ctttgcatgc gtttgtactg ctcaactacc gcacctgaat caaccatgac
    22021 agcgtcgatg gacgaaccac cgccgcctcc ggctgaagct tctatattct catcgaacga
    22081 actccagatc agcgccgcaa ataagatgac atgcagcact gctgaaataa ttatcgcccg
    22141 cttgagcttg tcgttttgtt cggttgcctt tgacactctc ggtttccaaa aactgttcgc
    22201 ctgttacccg ctctctttca agcaagggaa acgcagatgt ttagataggc tgcgtcatta
    22261 aaccaaccga tttcacaccc gcactatgta acaagttcag tgctttaatt atttcatcgt
    22321 aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc
    22381 tggacacttc cgccaccacc tgctctggtg gtaaacgctc caggcgatct ttctcaacca
    22441 ccacggtgta ctgaccaata ccagacactt caacaatcac tggcggatta tcgttactgc
    22501 tcaccgcctg tgattcagta gcgtctggca gatcgacctc cacgctctgg gtgatgatgg
    22561 gcgctgtcgc cataaagatc agcaacagca ccagcagtac gtccagcaac ggaacaatgt
    22621 tgatttcgga cttgagatcg cgacgacctc gtccacgcgc tctggccatg gcttacccct
    22681 tgttgctctc gctaacggta aacgcctggc ggtgcagaat cgcggtaaac tcttccataa
    22741 agttgtcgta attcagttcc agtttgttta cgcgctggtt gagacggtta taagccatta
    22801 ccgccggaat tgcggcgaac agaccaattg ccgtcgcaat caacgcttct gcgataccgg
    22861 gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag gcgtgcatga
    22921 tcccccagac cgtaccaaac agaccaatat acgggctgat ggagccaacc gtaccgagga
    22981 acggaatgtg cgtttccaga ttttcaagtt cacggttcat ggagatacgc atagcacgcg
    23041 acgccccttc cactacggct tccggcgcat ggctattggc acgatgcagg cgcacaaact
    23101 ctttgaaccc gctgtaaaag atttgttccg aaccagtcag attatcccgt ttcccctgac
    23161 tctcttgata gaggcgagag agttcgattc cagaccagaa tttatcttca aacgcttcgg
    23221 cttcacgcgc cgctgcgtta agaatacggg ttcgctggat aataatggcc caggatgcga
    23281 ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag aaggctagcc ttcaggaaca
    23341 aatcaaggat attcatgtca gtcactgctt aaactccgcg acaatagact tgggaagcgc
    23401 acgaggcttc attttgagtg ggtcaacgca aacaaccaga acctctgctt cattcagcaa
    23461 agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt
    23521 tatttcagtc tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt
    23581 cattttacgt accacaaagg caacgcgttc agccatcagc gcctgctgac tgaagtgatg
    23641 atgacgcagc atctctgtgc gtgctctttc ataaaaagcg acgtaactgg cgtggtacac
    23701 cacaccaccg gcatcggtat cttcatagta gacgcgaacc ggccatcgaa acagcgttgt
    23761 attcacttta catcccggta atgcaacaaa agttagagct tttaaacgct gctactatac
    23821 gcgcgcgatt gaggtttggg aatgagggca agttaagggc gcgtaaaaat ttatgggcct
    23881 ggagcggccc ataatttttt aaagagaatt ctgatttaaa agaagaagaa aatcagcccg
    23941 acaatgagaa caatatcggc aagcaatggg caaaaaatcc cttgccaaag aaccttctgc
    24001 ggacgaaagc ccacgccgtg aatcacacca gcacagacgg cccacatcag caataaacca
    24061 tgccagattt ccagttcact ggtcttcgcg gcaaaacgag atgggtccca aaacatacat
    24121 cctgctaaca gaagcgccat cacgaaggaa agcgcccgca gggggcgctt gtccattacc
    24181 gcatacaagg tcgcgataat cttactcatc agatgtcttc ttgaccggct ttgcctgatt
    24241 cgacgtgttc aagcgccagc gcggtgatta ccccaaacga acaggcaaga agcgttccca
    24301 gaatccatgc gaaataccac atttttagct ccttacttag tacagagagt gggtgttacg
    24361 ttcaatatct tctttggtga tacgaccgaa cattttccag taacaccagg cggtgtagag
    24421 cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg tcagctggct
    24481 ggaagttgca tcccacattg tcagacttgc gttcatcatg gtgctggacg gcatcacaaa
    24541 cgggaacatt gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac
    24601 aaacgcccac gcggctttat ccatacgtgc agtcaggatg gtcagcagca gcagaaccac
    24661 acccagtgcc ggaatagccc acagaattgg cgtgttgttg aagttaacca gccatgcgcc
    24721 agcttcacga accacttctt tattcagtgg gttagaggct gcgtaatggt ccattgtcga
    24781 tttcacgaca taaccatcga taccgtacat cacccatacg ccagccagtg cgaaacagac
    24841 cagtgtcacc agcgcagcca cctgagccgt tgcacgggta cgcaggtgca gttcgcccac
    24901 ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc atccctacgc tcaccacgcc
    24961 tgccagcagg ccgaacgggt taagcaactg gaagaagtta ccggtgtagt acagacgcag
    25021 atattcatca acgttgaacg gtacgccctg caacaggtta ccgaacgcta caccaattac
    25081 cagcggcgga acgaagctac caatgaagat gccccagtcc cacatgttac gccagcgggt
    25141 ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg aagaacaaag acgccagcac
    25201 gaggatcatc gccacataga agccggagaa cgcagcggca tagaccatcg gccaggcagc
    25261 aaagagtgcg ccgcccgcgg tgatcagcca aacctggtta ccgtcccagt gtggtgcaat
    25321 ggagttaatc ataattcgac gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc
    25381 cacccccatg tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc caaccagcag
    25441 ccaccagata aaacgcaata cttcataatc gatcatttga cgactcctgt cttagcgtgc
    25501 cggctgagta gtcgtggaag actgctcaaa gtgatagcga ccggttttca ggctgcttgg
    25561 gccgaggcgt gcaaacttga acattaagaa caattctgcc accaggaaca gggtatacag
    25621 gccgcaaatc agcaccattg agaagatgag atcgcctgcg gtcagtgacg aattcgccac
    25681 agctgtcggc agcacttcac cgatagccca cggttggcgg ccatattcag ccacgaacca
    25741 gcccgcttct acagcaatcc acggcagcgg aataccgtac agcgcggcgc gcagaagcca
    25801 ttttttctcg ccaatgcggt tgcggatgac actccagaaa gagagcgcga tgattgccag
    25861 cagcaggaag ccacacgcca ccatgatacg gaacgcaaag tacagcggcg ctacacgcgg
    25921 gatggagtct ttggttgcct gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt
    25981 atagcgtttc agcagcagac cgtaaccgag gtctttcttc atgctattga actggtcacg
    26041 aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc agagagtacg ccttcatccc
    26101 gttacgaatg cgttcttcat gctgcaccat cagctctttc aggccgataa ccggggtatc
    26161 cacggaacgc gttgcaatga tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt
    26221 ctcctcttcc tgatcaggaa tgccgaacaa agtaaaggca gcaggcgcag gttgcgtttc
    26281 ccactcggct tcaatagcag ccagtttggt tttctgcacg tcgcccattt cgtagccgga
    26341 ttcatcaccc agaacaataa cagacagaac agcagccata ccgaagctgg cagcgatagc
    26401 aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc atataccatg cgctgatacc
    26461 gaggatgaac atcgcgccag tcacataacc agacgctaca gtgtgaacga atttcacctg
    26521 agcaaccggg ttaagcacca gctcggagaa gctcaccatc tccatacgca tagtttcaaa
    26581 gttgaaatcg gacgcgattg ggttttgcat ccagccgttc gcaaccagaa tccacagtgc
    26641 ggacaggttt gaaccgagcg ccaccagcca ggtgacacac atatgctgaa ctttacccag
    26701 acgatcccaa ccgaagaaga acagacctac aaaggtggat tcgaggaaga aggccatcag
    26761 accttcgatt gccagcggcg caccgaagat atcccctaca tagtgggaat agtaagacca
    26821 gttagtcccg aactggaact ccatggtcag accggtagcc acacccagag cgaagttgat
    26881 accaaacaac ttgccccaga acttggtcat atctttataa atctgtttgc cggagaggac
    26941 gtagaccgtt tccataatgg ccaggaggaa cgccataccg agcgtcagtg gcacaaaaag
    27001 gaagtggtac atcgcggtca aggcaaactg taagcgcgac agttcgacta tatctaacat
    27061 catgactcct tgctcatcgc atgaagactc cgagagtgaa ccccgttaga aagggtcaca
    27121 cgcatgcccc aatagaaatt tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa
    27181 aacggcgtaa agaaaggttg caaacatgtt aataaaaact caaattgatc ccacgtatat
    27241 attacgccgc aaaatcctta caataaacag gtttttgttg aagcaaattt gcatttttcg
    27301 acggtgatca atttatagct aaattgccgc ctttcagcca atttgatcga gaacaattta
    27361 tctctttttg atgcccattt ccaagactta tacattgata aatatcaatt tctacaaatt
    27421 atgttaatca tgtttcaaca aggtaacttt agtgatattt atgtttaaaa tgtgtgaatt
    27481 ataagactga aattagtttc ggttggcaat taaagataaa aagagacgaa gcaaaaacag
    27541 cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag tttcaggatt
    27601 attaataaag gcataaatct cccccccgcc gatattaaca tttaattaac ttataattat
    27661 attataatta actcacccgt ggttattatt ttgttaaaaa taaagccaca tatacgtagc
    27721 ttgagagtga agataaacac aaagttacct gatgcgctac gcttgccagt gtctacatca
    27781 tctctgcaat atattgaatt tgcctgcttt tgtagaccga ataaagcgtt catgccgtat
    27841 ccggcatgaa caaagcgccc tttgtcaaca atctggccgc gcatatgtgg cttcggtttg
    27901 attgctattc aggcaagccg gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc
    27961 gatagatttg aaccttgatt ttcctcggtg gtaatgtgtt catccatcat cgtttctgag
    28021 caagaaatca cctcgcgact gaaagcaaca gtcgcatcac aggttgctga ttcagccgga
    28081 ttaaacagcc gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg
    28141 catcccactg ggggcatttt caacaaacta taactttccg gcacgttgaa tccggctttg
    28201 ttgagcttca tcgcatccca tgggattttg ttgtaacact gtactggagt cagccatgct
    28261 cgcgcctgct gagctacacc agcggcggtt ggcgtaccgg tataactcaa taaacttagg
    28321 cgacaagaaa gcagaccacg tagttgtgag tccgggactg gcattttaat tcccgaaggc
    28381 cgcctaggcc ttaaaagcag atcttctttc cccagtaagc ccacgccgcg cagcaacgta
    28441 atggcaaagg ttttcttctc ttcaccgatg acttcaaatt cacgtagccc ttcgctaaag
    28501 acagccatgc cgttacgccc ttcctgtaag gctgcatagt tgagcatatt ccataccgga
    28561 accggcgctt ctttccagcc ttcttgctgc cagttgttca ttgcactgtc gttcacgggg
    28621 cgcgttagcg aaccaaactg ggtatctgcc agaacactat cggtgttaaa agatgtaggg
    28681 atcaggacgc gaaggcgatg atcgtcagcc tggttatcaa ggttgatatc cacatcaatg
    28741 cgcctgttgt tatgactaag agtgacaacc aactcgacat ctactctgcc agtggattgt
    28801 cttgcgctgc gttgttgcag attacgcggc accgccattt catagcggat aacagccctg
    28861 ctctgccagg cctcatgaat aatctcgcat tgcggtttcg cattcgccga agtaattacc
    28921 cactcttctt ttgcgggtga atagtcatat tcatcaccat catcagagct ttcttcaatt
    28981 tgcaacaccc ggtcatagcg cacaccgcta tctttatcta ccagttgtag cgttccatcc
    29041 tcattgagcg caatttgcca gaaagcattt tccagtatcc cttcagcgtc actttttgcc
    29101 gcaatcacgt tgccaggctg attcgcttcg atataaagcg tgcgatagcc catagaaggg
    29161 acaatctggt tgatctgtat atcaaactcc ataaagggat cgtaattacc gtaatgaact
    29221 atttgccgat cgattaggcc tggatcgatc tcacgggcat ggcgaataaa atacggtaca
    29281 ggctgaccgc gatcgtctcg tagattaaac tggctggcgc gcagccgcac agtggtgttg
    29341 atcacttctt cccgcggcca gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc
    29401 tgcggcatgt tgtcggcgat tttacgcata tagaaacgca gcagattatc cgccatgtct
    29461 tcagccagtt cgaagcgggc gacgatttcg cgatgaactt tgtcactaca gcagcagccg
    29521 atgctgtcgt gggcatgatt ttttaagatc tctttccaca ttttctccag caagccgtgg
    29581 tggtatccaa aacccaacgt cctggccagc gttgccagcg gttccagcag attaacaatc
    29641 ttattttcaa tacgtgcgtg ggcaactttg atatccatgc gcgtggaacc gatggtgcga
    29701 tgcacgcgca tatatttgcc atcaataaat tcccctttca gggttgccag attatctcgc
    29761 tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt tcgttgagga
    29821 taaatttcgc gcagtttctc catcacttcg aagatatttt gctgcaatgg catctgatca
    29881 tgcccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac gtcaaaataa
    29941 ctgtcgaggc gtttgcgtaa tccgttttcg tccgcaggta agtacttacc gatggcataa
    30001 cccaacggca tcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg
    30061 gttttatccg taccgtggcg ctcagaacat ccacgccaga acatggtgca ggtaatgcca
    30121 aatccattgt agatatgcgg cagttgcccg gacatgccaa aggaatcggg taaataacct
    30181 attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag attacggacg
    30241 atggattccg cagaaacaat cgtggtatcg gtctgggtat actaggggcc gataatcaac
    30301 ttgccggctt ctaccagttt cttcacgcgg tctttgtttt ccggtttcac cgcgaaataa
    30361 tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc
    30421 agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc ttcggtggtg
    30481 aaataccact cccgatccca gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc
    30541 atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg ccatgcttaa
    30601 ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc accaatgccg
    30661 caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga agtaaaaaga
    30721 gcgagaaaat gccagcaccg ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc
    30781 ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct tcaatcgcca
    30841 tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat ttcccggtct
    30901 caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg
    30961 ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg
    31021 cgcccaggca gaatgcataa gcggctttat tcactggccc tccaaggtca aaggaacaca
    31081 taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg ttcagccagg
    31141 cggtaagcga gttattgatc caggcgacag gttcccccac cacaaacagc atcagactgc
    31201 ccgctcccaa tgtaccgagc accgggtaga gataaaaagt caggaagcca ttgaatttac
    31261 tgctaagacg caagtgattt ttcatccagc gtatcaagta accggctatc aatccgccaa
    31321 cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc gcaaagcctg
    31381 gcgctaacgc cggtttatct gccagcgaat aggcggtata ggctgccagc accggtacca
    31441 tcaaaattcc gagcagcccg ccgcccagtt tgcggtacat ccacagccag gagttttctt
    31501 cattaaacag atcttgtagc ccgaagattt gcgacagtaa taccgcgacc gccagcaccg
    31561 tcccccccgc gacaatcaac ggtacggcaa aagagatccc gctcaacagt gcctgtttca
    31621 gctccgtttt gacgcttttc accggttgcg tatcttgttg taaggtacgc gtctcatcgc
    31681 cacgctcgag ggtaagcgat tgttggatca acgcttctgc atggcgaatc ggctcggcaa
    31741 caggcacaga aagcgcagga atgccgttaa aacgctcact ctccttgatg gcgacttcag
    31801 ccgcaaaaat acaggcagtt gcactattga gttgatccgc cgttaaacgc ccttcaatac
    31861 cgttagcacc ttgcttttca acgtagacat tcacgccgag tttgcgtccg gctttttcca
    31921 gatactccgc agccatgtag gtgtgagcaa tacccgccgg gcaggccgtt acgcaaacga
    31981 tagttggcgc gttggaaaaa gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg
    32041 agagcaattc atcaggcgtc gtcgccgact gtatacgcgc ccgaatctca tcatccgcca
    32101 ggcgcgtggt cagcgctgtc agcagttgca tgtgcgtagt acccgcttca ttgaggggaa
    32161 tcgccagcaa caccactaaa tcaaccgctt ccgggccatc cacgccttcc cactgaagcg
    32221 gctcgctgag tgtggcgacc gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg
    32281 gcacagccaa cccttcactt aaggcggtcg ggccaaggct ttcacggcga tacacttctt
    32341 tcagaaattg ctcagtactg gaaattttcc ccagagcagc aagacgttga gtcaacgcgt
    32401 gaatggcctc ttcacggctg gtaaagcgcg cattcagaca caacgcatcg cggtgggtca
    32461 gagtcgtcag gttcatcatg atcctcactt gatcgttttt atagtcgcgc cagtgtgccc
    32521 gacaaaacaa taccattgcg taatcaattt cacttgattg aaattatttg cattttattt
    32581 gtattgttta caggatgaat ttatcgggca taatggctaa attcgtatta atgagatgct
    32641 aaataatggg acacaaaccc ttataccgac agattgctga tcgcattcgt gagcaaattg
    32701 cgcgtggcga gcttaaacct ggcgatgcgt tacctaccga atcggcatta caaacagagt
    32761 ttggcgtcag ccgggttacg gtgcgtcagg ctttgcgcca gttagtcgag caacagatcc
    32821 tcgaaagcat tcagggcagc gggacttacg tcaaagaaga gcgggtcaat tacgatattt
    32881 ttcagttaac cagttttgat gaaaaactgt cggaccgtca cgtcgataca cacagtgaag
    32941 ttctgatatt tgaagtgatt ccggctgacg attttcttca gcaacagcta caaatcaccg
    33001 tgcaggatcg tgtgtggcat gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc
    33061 tggaagaaac ctggatgccg cttgcgttgt tcccggatct cacctggcag gtcatggaaa
    33121 attcgaaata tcactttatt gaagaagtga agaagatggt tatcgatcgt agcgaacagg
    33181 aaatcattcc tctgatgcca accgaagaga tgagccgtct gcttaatatc agccagacaa
    33241 agccgattct tgaaaaagta tcgcgtggat atttagttga tggtcgggtg tttgaatata
    33301 gccgcaacgc ttttaatacc gatgactata aatttaccct gatagcgcag cgaaaatcat
    33361 cgcgataagc acaaaaaggc catcatacga tggccaacca tgtcgaaacc gacagccctt
    33421 cgccattcct gtagagggaa gcggcgaggg ctatttctta ttacagatat ttatttcaga
    33481 acagttttca gtgcttcacc gatatccgcc aggctgcgaa cggttttcac gcctgcggct
    33541 tccagagcag cgaatttctc atccgcagtc cctttcccac cggcaatgat cgcacccgcg
    33601 tggcccatac gtttgccttt cggcgcagtc acaccagcga tgtaacccac aactggcttg
    33661 gtaacatgct ctttgatgta cgctgccgct tcttcttcag cgctaccgcc gatctcaccg
    33721 atcatcacga tcgcttcggt ctgcggatct ttttcgaaca tttcgagaat gtcgataaag
    33781 ttagagccag ggatcgggtc accgccgata ccgacacagg tcgactgacc gaaaccgtaa
    33841 tccgtggtct gtttaaccgc ttcataggtc agtgtaccgg aacgggaaac gatacccact
    33901 ttacccggtt tgtgaatgtg acccggctgg ataccaattt tgcattcacc cggggtgata
    33961 acgcctgggc agttcgggcc gatcatacga acgcctgctt cgtccagttt cactttcacg
    34021 gtcagcatat ccagcgtcgg gatgccttca gtgatggtga taatcagttt gatgcctgca
    34081 tcgatagctt ccagaatgga gtctttgcag aacggtgctg gtacgtaaat aacagacgcg
    34141 gaagcgccag tagcagcaac agcttcacgc acggtgttga acaccggcag gccgaggtgg
    34201 gtagtgccgc ctttacctgg agttacgcca ccaaccattt tagtgccgta tgcaatggcc
    34261 tgttctgagt ggaaagtccc ctggctaccg gtaaagccct ggcagataac cttggtgttt
    34321 ttatcaatta aaatggacat tatttcccct ccactgcggc aacaacctgc tgagctgcat
    34381 ccgtcagacc ttttgctgca ataatattca ggccgctgtc agccagtttc ttcgcgccga
    34441 gttcggcgtt gttaccttcc agacgtacca cgaccggtac gttaacaccc acttccgcta
    34501 ccgcgccgat gataccgtca gcgatcaggt cgcaacgaac gataccgccg aagatgttaa
    34561 ccagaacggc tttcactttg tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt
    34621 ctttggttgc gccgccgcca acgtccagga agttagccgg ttcgccgccg tgcagtttaa
    34681 ctatgtccat cgtacccatc gccagacctg cgccgttaac catacaaccg atgttaccgt
    34741 ccagcgcaac gtagttcagt tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg
    34801 actggtcacg catttcgcgc agatcaggct ggcggaacag ggcgttgcca tcagcgccca
    34861 gtttgccgtc gaggcaaatc agatcgccct gtttggtgat gaccagcggg ttgatttcga
    34921 tcagcgccag gtcgcgctcc aggaaaatgg tcgccaggcc catgaagatt ttggtgaact
    34981 gctgaaccag tttaccttcc agacccagtt tgaacgccag ctcgcgtccc tgatacggca
    35041 tcgggccagt cagcggatca agcgcaactt tatggatcag gtgcggagtt tcttccgcca
    35101 ctttttcgat ttccacgccg ccttcggtgg aggccataaa gaccacgcga cgggaactac
    35161 ggtctacaac ggcaccgaga tacagctctt tagcgatatc ggtcgccgct tcaaccagaa
    35221 tctggttaac cggttggcca ttggcatctg tttgatacgt taccagacgc ttgcccagcc
    35281 agttttctgc aaaagcacgg atgtcttctt tgctgtttac aactttcaca ccgcccgctt
    35341 taccgcggcc accagcgtga acctgacatt tcactaccca cggaccggca ccgatttttg
    35401 aagcggcttc ttctgcttcg cgcggagtag tacaggcata acccaccggt gctggtaagc
    35461 catagcgggc aaacagttgt tttgcctgat attcatgtaa gttcatgtgt tctgtccatc
    35521 cttcagtaat cgttatcttt taaaccgtag accgacaccc tatccacgtc ggtctacagt
    35581 gcaggtgaag cttaaactac tacacgtcca gcagcagacg cgtcggctct tccagtaact
    35641 ctttgatcgt taccaggaag cccacggatt cgcgaccatc gatcagacgg tgatcgtagg
    35701 acagcgccag gtacatcatc ggcaggatct caacctgacc attcaccgcc atcggacgat
    35761 ctttgatagc gtgcataccc agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca
    35821 tcagggaacc gaacacacca ccgttggtga tggtgaagtt accaccggtc agatcttcaa
    35881 ccgtcagctt gccatcacgg cctttgactg ccagctcttt gattttcttc tcgatgtctg
    35941 ccatgccgag ggtatcgaca tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa
    36001 ccgccatgct gacgtcgaaa tagttgtggt aaaccacgtc atcgccgtcg atagacgcgt
    36061 tcacttccgg gtaacgtttc agggcttcaa ccaccgcttt cacgtagaag gacataaagc
    36121 ccagacggat gccgtggcgt ttttcaaacg cttcaccgta ctgcttacgc agatccataa
    36181 tcggcttcat gttgacttcg ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca
    36241 gcagacgctc tgccacacgc ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg
    36301 cagccagagc cggttgcgcc gccggagcag ccgctgccgg agcagactct ttcgccgggg
    36361 ctttcgccag atgtttttcc acatcttcac gagtcagacg accacccaca ccggtgcctt
    36421 taatggcgct ggcgtcgaga ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg
    36481 catcgttgtt ttgctcttcc agagacgcct gctggcgttg cgccggagtg gacgctttct
    36541 cttcagattt ggcgctggtt tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga
    36601 tctgacgaga cgttaccgtt gtaccttcat cttccagaac cgcatccaga atgccgtctg
    36661 ctgatgccgg tacttccagt accactttgt cagtttcgat ttctaccagc acttcatcac
    36721 gtacgactgc gtcgccgggt tttttatgcc aggttgcgac ggtggcatcg gctacggatt
    36781 caggcaggtc agggaccaga atatctacgc tactcattgt gtatccttta tttattcgac
    36841 gttcagcgcg tcattaacca gatcttgttg ctgtttctgg tgaacggaca tataccctac
    36901 cgccggagag gcggaggccg ggcggcctgc ataacgcaga gaagccccaa acggaatcac
    36961 ttcacggaaa tgatgctggc tgcagtacca tgcgccctgg ttgagcggct cttcctggca
    37021 ccagacaaaa tccttgacgt gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg
    37081 gaacgggtag agttgctcga tacgcacaat ggcgacatcg tgttgattgt tcttacgacg
    37141 ctgttccagc aggtcgtaat aaaccttacc agaacacatc actacgcgct tcacgccctt
    37201 cggatcaagc tcgtcgattt caccgatggc tggcaggaag gtgccgttcg ccagttcttc
    37261 gaggctggaa accgccagcg gatgacgcag cagggatttc ggcgacatca cgaccagcgg
    37321 acgacgcatc ccgcgcagcg cctgacgacg cagcatgtgg taaacctgtg ccggggtaga
    37381 cggtacgcaa acctgcatgt tttgctcagc acaaagttgc agataacgtt ccagacgcgc
    37441 ggaggagtgc tccggcccct gcccttcgta accgtgcggc agcaacatca ccagaccaca
    37501 catccggccc catttctgct cgccagagga gatgaactgg tcgataacca cctgcgcacc
    37561 gttggcgaag tcaccgaact gcgcttccca gatggtcagg gtgcgtggtt ctgcggtggc
    37621 ataaccatat tcaaacgcca gcacggcttc ttctgacagt acggagtccc agacacggaa
    37681 cgcgccctga ccgttatgga tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt
    37741 gtggatcacc gcgtggcggt ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg
    37801 aaccggaatg ccttcatcaa ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc
    37861 gaacagtttc tcaccggctg ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga
    37921 ctgcatttca actgcttctg gtaccgtact gatacgttta gccagttcct gcaggcgctt
    37981 catctcaact ttgttcgggt actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt
    38041 gaaagagtgc atgttcatcg gacgccactc tgccactacg caatcgccag catccagcgc
    38101 atcgcggtac aggttaacca tctcggtggc atcttccagc gtcgccactt tttcctgctc
    38161 cagcttgtca gcgtagattt tgcgcggtgt cggatgtttt ttgattttct gatacatcag
    38221 tggctgggtt gcgctcggct cgtcggcttc gttgtggccg tgacggcggt agcataccag
    38281 gtcgatgaag acatcacgtt taaaggtgtt acggaaatcg agcgccagac gggtcacaaa
    38341 ggcaacggct tccggatcat ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc
    38401 gatatcagta cagtacggtg tagaacgggc atccagcggg ttagaggtgg tgaaaccaac
    38461 ctggttgttg ataacgatac gtaccgtacc gccaacttca taaccacgcg ctttcgacat
    38521 gttcagagtt tcctgaacca cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat
    38581 tggcagcact ttgttgctgt tcggctcatc aagtctgtcc agacgggcac gaacagagcc
    38641 gataacgacc gggcttacaa tctcaaggtg agacgggtta aacgccagcg ccaggtgcac
    38701 caggccgcca tcggtctgga agtcagacga gaagcccatg tggtatttca cgtcgcctgt
    38761 gccgaggtgt tctttatgtt taccggcgaa ctcgtcgaac aagtcttgcg gttttttacc
    38821 cagcacgttc accagcacgt tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg
    38881 ggtgccgctg ttgccagcgt ggcggatcat ctctttaagc atcgggatta acgcgtcacc
    38941 gccttccagc gagaagcgtt ttgcgccagg gaattttgcg ccgaggtaac gctcaaggcc
    39001 ttcagcggcg gtcagttcgc ttaagaagcg ttttttctct tcgctattga aagtcgcgcg
    39061 accagactcg atacgctgtt ggatccagcg tttttcttcg gtgctggtga tgtgcatata
    39121 ctcggcacca atcgggccgc agtaggtttg cttgagggcg gaaataagct cgccgagttt
    39181 catggtttct ttgccgctgg caaatgaacc gacgttgaag gtttcctgga agtctgcttc
    39241 ggtcagatcg tggaaagacg gatccagatc ggccacttta tcttgctgcc acagtcccag
    39301 cggatctaga ttcgcatgct ggtgaccacg gaagcggtat gcgttaatga gctgcaggac
    39361 tttaacctgc ttcacattgg tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc
    39421 tttcgccagg cggcggaaat attcacgcgt ttgagagtgg aattgatccg gtttgactcc
    39481 cgtaccaggt aactgctgga acgtcgaacg ccagttagcg tcaaccgagt caggatcggt
    39541 taagaagtct tcatagagct gttctatcca gctctggttt gcgccagaga ggtaagaaga
    39601 gtccaaccag gctttcaaag cgctgttctg catcgtgatc ccttaagcat cttttttatg
    39661 cttacttcgc cgtggatact accacgcaca gtgatgtgcg tgccggggtt cacttgcgag
    39721 ctcttgtcta cgtagtggct cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca
    39781 gtttttaaag atttcctgca tttctttgtt acgcctgatg cgctgcgctt atcaggcctg
    39841 cgggtaatga cttgtaggcc ggataaggcg ttcacgccgc atccggcact ggttgcctga
    39901 tgcaacgctt gcgcgtctta tcaggcctac agtttacgca ttacgttgca acaacatcga
    39961 cttgatatgg ccgatggcgc gcgtcgggtt cagccccttc ggacatacac tgacgcagtt
    40021 catgatgctg tgacagcgga atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc
    40081 agtctcggta tcacggctat cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc
    40141 gataaactta tcgggattcc accagaaaga cggacaagag gttgaacaac atgcgcagag
    40201 aatacattca tacagcccgt cgagtttttc gcgctgctct ggcatctgta aatgctcgcg
    40261 agctggcgga ttttgtccat tattcaacag gtaaggctta attttctcat attgcgcata
    40321 gaattgtccc atgtctacca ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat
    40381 cacaatcttc ttgcccggct ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt
    40441 cttgccgttc atgttcagac cgtcggaacc gcacacacct tcacggcagg agcggcggaa
    40501 cgacaggctg ggatctttct ctttcagctg gataagcgca tccagcagca tcatgtcgcg
    40561 accttcttcc gcttccaggg tgtaatcctg catacgcgga gcatcatcaa catccgggtt
    40621 atagcgataa attgaaaact cgagtctcat tttcctgtct ccgcattagt aagtacgaat
    40681 cttcggcggg aatgccgggc gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga
    40741 ttccgactca ggcagataca gggagtggca cagccagttt tcatcatcac gatccgggaa
    40801 gtcgaagcgg ctatgtgcgc cacggctttc ggtacggaag ttggcagaaa cagccgttgc
    40861 atacgccgtt tccatcaggt tatccagttc caggcactca acgcgctggg tgttgaactc
    40921 gctggaagtg tcatccagac gggcattttt cagacgctcg cggatcactt tcaactgctc
    40981 aagccctttc gccatcgcat caccttcacg gaagaccgag aagttatgct gcatacattc
    41041 ttgcagcgct ttacggatcg ccaccggatc ttcaccgtta cgattattgt tccaccggtt
    41101 caggcgatcc agagacgctt caacatcaga ctcgctggca tcgtgcagtg cgccctgctc
    41161 ggcgatagac tcttgcagat gcagacctgc cgcgcgacca aagaccacca ggtccagcag
    41221 cgagttgccg cccagacggt tagcgccgtg taccgataca caagcgattt caccaacggc
    41281 aaacagtccc ggaacaacca catcttcgcc tttctcattc acagtcagcg cctgaccggt
    41341 aactttggtc ggaataccgc ccatcatgta gtgacaggtt gggataaccg gaatcggctc
    41401 tttcaccgga tcgacgtgag cgaaggtacg ggaaagctcc aggatacccg gcagacggga
    41461 ttcgagaact tctttaccca ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc
    41521 atcacagccg cgaccttcac ggatttcaat catgatggaa cgcgccacca catcacggcc
    41581 cgccaggtct ttggcgttcg gcgcataacg ctccataaag cgttcgccat gtttgttcag
    41641 cagataaccg ccttcaccac ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc
    41701 ggtcgggtgg aattgccaca tttccatatc ctgcaccggt acgcctgcgc ggatagccat
    41761 gccgacaccg tcgccagtgt taatgtgggc gttagtggtg gactgataaa tacgccctgc
    41821 tccgccagtc gccagcacgg tagcgcgggc tttgaaataa accacttcac cggtttcgat
    41881 gcacagtgcg gtacaaccca ccaccgcgcc atcctggttt ttcaccagat ccagcgcata
    41941 ccactcggag aaaatggtgg tgtggttttt caggttctgc tgataaagcg tgtgcaacag
    42001 tgcgtgaccg gtacggtcag cagccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt
    42061 tttcgactga ccgccaaacg gacgttgata gatacgacca tcatcgagac gcgagaacgg
    42121 caggcccatg tgttcgagtt ccagaatcgc ttccggcccg gttttacaca tatattcaat
    42181 cgcgtcctgg tcaccgatat agtccgaccc ttttacggtg tcgtacatat gccattccca
    42241 gttatcttca tgggtattac ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg
    42301 ggaacgggtc gggaagactt tagagagcag ggcacaggtc tggccgctct gggaaatttg
    42361 cagcgccgcg cgcatacctg cgccaccggc accaatcaca actgcatcaa attctctgac
    42421 tggcaatttc atcacacacc ccacaccaca acgaatccat aaatcacgta aaccaccagt
    42481 gcaacgacaa tcaccagttg cagcatcagg cgcaaggcca gcggtttaac gtagtcggtc
    42541 aacacctgcc acatgccgat ccaggcatga atcaagatag aaaacagcgc cagcagggtg
    42601 aacactttgg tgaacgcaga ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca
    42661 ctggtggcga aaaaaccgac catataaatg atgtagagcg tcaggacgat agcggtagcg
    42721 cgaacgagga tgaaatcatg tacgccattg cgtcctaatg cggaggcgtt gcttaccata
    42781 cgaggactcc tgcgagaagt gaaagcacga cagtaataac aaaggagatt ttggcggagc
    42841 gtttacccgc ttcgaatgtt tcttccagat agccaaaatc catcatcatg tggcgaatac
    42901 ctacgacgac gtgatacgcc agagcggtaa ggatgcccca catgataaat ttgacgaaga
    42961 agctgcccat aatcgcggaa gcttgctcga aaccttcagg ggaagagagg ctggtaccca
    43021 gaagccactg caggatgccc actgcaacaa aggtgatcac accggaaacg cgatggagaa
    43081 tggacgctat cgccgtgatg gggaaccgga tggtctgtag gtccagatta acaggtcttt
    43141 gttttttcac atttcttatc atgaataacg cccacatgct gttcttatta ttccctgggg
    43201 actacgggca cagaggttaa ctttctgtta cctggagacg tcgggatttc cttcctccgg
    43261 tctgcttgcg ggtcagacag cgtcctttcc ataactgcgc gtcatgcaaa acactgcttc
    43321 cagatgcgaa aacgacacgt tacaacgctg ggtggctcgg gattgcaggg tgttccggag
    43381 acctggcggc agtataggct gttcacaaaa tcattacaat taacctacat atagtttgtc
    43441 gggttttatc ctgaacagtg atccaggtca cgataacaac atttatttaa tttttaatca
    43501 tctaatttga caatcattca acaaagttgt tacaaacatt accaggaaaa gcatataatg
    43561 cgtaaaagtt atgaagtcgg tatttcacct aagattaact tatgtaacag tgtggaagta
    43621 ttgaccaatt cattcgggac agttattagt ggtagacaag tttaataatt cggattgcta
    43681 agtacttgat tcgccattta ttcgtcatca atggattctt tacctgcaag cgcccagagc
    43741 tctgtaccca ggttttcccc tctttcacag agcggcgagc caaataacaa acgggtaaag
    43801 ccaggttgat gtgcgaaggc aaatttaagt tccggcagtc ttacgcaata aggcgctaag
    43861 gagaccttaa atggctgata caaaagcaaa actcaacctc aacggggaca cagctgttga
    43921 actggatgtg ctgaaaggca cgctgggtca agatgttatt gatatccgta ctctcggttc
    43981 aaaaggtgtg ttcacctttg acccaggctt cacttcaacc gcatcctgcg aatctaaaat
    44041 tacttttatt gatggtgatg aaggtatttt gctgcaccgc ggtttcccga tcgatcagct
    44101 ggcgaccgat tctaactacc tagaagtttg ttacatcctg ctgaatggtg aaaaaccgac
    44161 tcaggaacag tatgacgaat ttaaaactac ggtgacccgt cataccatga tccacgagca
    44221 gattacccgt ctgttccacg ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg
    44281 tattaccggc gcgctggcgg cgttctatca cgactcgctg gatgttaaca atcctcgtca
    44341 tcgtgaaatt gccgcgttcc gcctgctgtc gaaaatgccg accatggccg cgatgtgtta
    44401 caagtattcc attggtcagc catttgttta tccgcgcaac gatctctcct atgccggtaa
    44461 cttcctgaat atgatgttct ccacgccgtg cgaaccgtat gaagttaatc cgattctgga
    44521 acgtgctatg gaccgtattc tgatcctgca cgctgaccat gaacagaacg cctctacctc
    44581 caccgtgcgt accgctggct cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat
    44641 tgcttcactg tggggacctg cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga
    44701 agaaattagc tccgttaaac acattccgga atttgttcgt cgtgcgaaag ataaaaatga
    44761 ttctttccgc ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac
    44821 cgtaatgcgt gaaacctgcc atgaagttct gaaagagctg ggcaccaaag atgacctgct
    44881 ggaagtggct atggagctgg aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa
    44941 actgtacccg aacgtcgatt tctactctgg tatcatcctg aaagcgatgg gtattccgtc
    45001 ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg cccactggag
    45061 cgaaatgcac agttacggta tgaagattgc ccgtccgcgt cagctgtata caggatatga
    45121 aaaacgcgac tttaaaagcg atatcaagcg ttaatggttg attgctaagt tgtaaatatt
    45181 ttaacccgcc gttcaaatgg cgggttaatt tttatatgcc taaacacaaa aaattgtaaa
    45241 aataaaatcc actatcggac attcatatat tctaaaaaaa aatacatcgg tataaattat
    45301 aaacaaatag atg
//
LOCUS       DAECNO010000025        43547 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.025, whole genome shotgun sequence.
ACCESSION   DAECNO010000025 DAECNO010000000
VERSION     DAECNO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.025"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            311..1744
                     /gene="cscA"
                     /locus_tag="KKO08_001376"
     CDS             311..1744
                     /gene="cscA"
                     /locus_tag="KKO08_001376"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBD6317688.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1752..2810)
                     /locus_tag="KKO08_001377"
     CDS             complement(1752..2810)
                     /locus_tag="KKO08_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD6317689.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2978..3196
                     /locus_tag="KKO08_001378"
                     /pseudo
     CDS             2978..3196
                     /locus_tag="KKO08_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3214..4542
                     /gene="dsdA"
                     /locus_tag="KKO08_001379"
     CDS             3214..4542
                     /gene="dsdA"
                     /locus_tag="KKO08_001379"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBD6317690.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4650..6188)
                     /gene="emrY"
                     /locus_tag="KKO08_001380"
     CDS             complement(4650..6188)
                     /gene="emrY"
                     /locus_tag="KKO08_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBD6317691.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6188..7351)
                     /gene="emrK"
                     /locus_tag="KKO08_001381"
     CDS             complement(6188..7351)
                     /gene="emrK"
                     /locus_tag="KKO08_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBD6317692.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7767..8381
                     /gene="evgA"
                     /locus_tag="KKO08_001382"
     CDS             7767..8381
                     /gene="evgA"
                     /locus_tag="KKO08_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBD6317693.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8386..11979
                     /gene="evgS"
                     /locus_tag="KKO08_001383"
     CDS             8386..11979
                     /gene="evgS"
                     /locus_tag="KKO08_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBD6317694.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(12035..13180)
                     /gene="yfdE"
                     /locus_tag="KKO08_001384"
     CDS             complement(12035..13180)
                     /gene="yfdE"
                     /locus_tag="KKO08_001384"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBD6317695.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13254..14198)
                     /gene="yfdV"
                     /locus_tag="KKO08_001385"
     CDS             complement(13254..14198)
                     /gene="yfdV"
                     /locus_tag="KKO08_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBD6317696.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14268..15962)
                     /gene="oxc"
                     /locus_tag="KKO08_001386"
     CDS             complement(14268..15962)
                     /gene="oxc"
                     /locus_tag="KKO08_001386"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBD6317697.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(16016..17266)
                     /gene="frc"
                     /locus_tag="KKO08_001387"
     CDS             complement(16016..17266)
                     /gene="frc"
                     /locus_tag="KKO08_001387"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBD6317698.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17779..18402)
                     /locus_tag="KKO08_001388"
     CDS             complement(17779..18402)
                     /locus_tag="KKO08_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBD6317699.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18699..18974
                     /locus_tag="KKO08_001389"
     CDS             18699..18974
                     /locus_tag="KKO08_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317700.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(19051..19293)
                     /locus_tag="KKO08_001390"
     CDS             complement(19051..19293)
                     /locus_tag="KKO08_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBD6317701.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19646..20566
                     /gene="lpxP"
                     /locus_tag="KKO08_001391"
     CDS             19646..20566
                     /gene="lpxP"
                     /locus_tag="KKO08_001391"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBD6317702.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20920..20991
                     /gene="ypdK"
                     /locus_tag="KKO08_001392"
     CDS             20920..20991
                     /gene="ypdK"
                     /locus_tag="KKO08_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBD6317703.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(21056..22294)
                     /gene="alaC"
                     /locus_tag="KKO08_001393"
     CDS             complement(21056..22294)
                     /gene="alaC"
                     /locus_tag="KKO08_001393"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBD6317704.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22671..24157
                     /locus_tag="KKO08_001394"
                     /pseudo
     CDS             22671..24157
                     /locus_tag="KKO08_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24172..24906
                     /gene="ypdB"
                     /locus_tag="KKO08_001395"
     CDS             24172..24906
                     /gene="ypdB"
                     /locus_tag="KKO08_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBD6317705.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24919..25776
                     /locus_tag="KKO08_001396"
     CDS             24919..25776
                     /locus_tag="KKO08_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6317706.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25779..28274)
                     /gene="ptsP"
                     /locus_tag="KKO08_001397"
     CDS             complement(25779..28274)
                     /gene="ptsP"
                     /locus_tag="KKO08_001397"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBD6317707.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQNILTLLETELTF"
     gene            complement(28299..29336)
                     /gene="ypdE"
                     /locus_tag="KKO08_001398"
     CDS             complement(28299..29336)
                     /gene="ypdE"
                     /locus_tag="KKO08_001398"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBD6317708.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29336..30419)
                     /gene="ypdF"
                     /locus_tag="KKO08_001399"
                     /pseudo
     CDS             complement(29336..30419)
                     /gene="ypdF"
                     /locus_tag="KKO08_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30434..31681)
                     /locus_tag="KKO08_001400"
     CDS             complement(30434..31681)
                     /locus_tag="KKO08_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBD6317709.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31703..32029)
                     /locus_tag="KKO08_001401"
     CDS             complement(31703..32029)
                     /locus_tag="KKO08_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBD6317710.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32248..33213)
                     /gene="glk"
                     /locus_tag="KKO08_001402"
     CDS             complement(32248..33213)
                     /gene="glk"
                     /locus_tag="KKO08_001402"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBD6317711.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33417..34673
                     /locus_tag="KKO08_001403"
     CDS             33417..34673
                     /locus_tag="KKO08_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBD6317712.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34788..35114
                     /locus_tag="KKO08_001404"
     CDS             34788..35114
                     /locus_tag="KKO08_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBD6317713.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35255..36493)
                     /locus_tag="KKO08_001405"
     CDS             complement(35255..36493)
                     /locus_tag="KKO08_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBD6317714.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36829..38031
                     /gene="nupC"
                     /locus_tag="KKO08_001406"
     CDS             36829..38031
                     /gene="nupC"
                     /locus_tag="KKO08_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBD6317715.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38081..40193)
                     /locus_tag="KKO08_001407"
                     /pseudo
     CDS             complement(38081..40193)
                     /locus_tag="KKO08_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40401..40476)
                     /locus_tag="KKO08_001408"
     tRNA            complement(40401..40476)
                     /locus_tag="KKO08_001408"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40441..40443),aa:Ala,seq:ggc)
     gene            complement(40516..40591)
                     /locus_tag="KKO08_001409"
     tRNA            complement(40516..40591)
                     /locus_tag="KKO08_001409"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40556..40558),aa:Ala,seq:ggc)
     gene            40812..41171
                     /locus_tag="KKO08_001410"
     CDS             40812..41171
                     /locus_tag="KKO08_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD6317716.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41173..41565
                     /locus_tag="KKO08_001411"
     CDS             41173..41565
                     /locus_tag="KKO08_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBD6317717.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41617..43032)
                     /gene="gltX"
                     /locus_tag="KKO08_001412"
     CDS             complement(41617..43032)
                     /gene="gltX"
                     /locus_tag="KKO08_001412"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBD6317718.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43291..43366
                     /locus_tag="KKO08_001413"
     tRNA            43291..43366
                     /locus_tag="KKO08_001413"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43324..43326,aa:Val,seq:tac)
     gene            43411..43486
                     /locus_tag="KKO08_001414"
     tRNA            43411..43486
                     /locus_tag="KKO08_001414"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43444..43446,aa:Val,seq:tac)
BASE COUNT        11581 a        10839 c        10109 g        11018 t
ORIGIN      
        1 aatccggaaa caagtgagcg tttccggatt cttacacagc cactggatcg gtcaactgat
       61 ccttaactga tcggcattaa tactttggct gacatttttt tcccttaaat tcatctgagt
      121 tacgcatagt gataaacctc tttctcgcaa aatcgtcatg gatttactaa aacatgcata
      181 ttcgatcaca aaacgccata gttaacgtta acatttgcga tattcatcgc atttatgaaa
      241 gtaagggact atatttttat aaaagttaac gttaataatt caccaaattt gcttaaccag
      301 gatgattaaa atgacgcaat ctcgattgca tgcggcgcaa aacgcactag caaaacttca
      361 tgagcaccgg ggtaacactt tctatcccca ttttcacctc gcgcctcctg ccggatggat
      421 gaacgatcca aacggcctga tttggtttaa cgatcgttat cacgcgtttt atcaacatca
      481 cccgatgagc gaacactggg gaccaatgca ctggggacat gccaccagtg acgatatgat
      541 ccactggcag catgagccta ttgcgctagc gccaggagac gataatgaca aagacgggtg
      601 tttttcaggt agtgctgtcg atgacaatgg tgtcctctca cttatctaca ccggacacgt
      661 ctggctcgat ggtgcaggta atgacgatgc aattcgcgaa gtacaatgtc tggctaccag
      721 tcgggatggt attcatttcg agaaacaggg tgtgatcctc actccaccag aaggaatcat
      781 gcacttccgc gatcctaaag tgtggcgtga agccgacaca tggtggatgg tagtcggggc
      841 gaaagatcca ggcaacacgg ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg
      901 ggccttcgat cgcgtactgg cccacgctga tgcgggtgaa agctatatgt gggaatgtcc
      961 ggactttttc agccttggcg atcagcatta tctgatgttt tccccgcagg gaatgaatgc
     1021 cgagggatac agttaccgaa atcgctttca aagtggcgta atacccggaa tgtggtcgcc
     1081 aggacgactt tttgcacaat ccgggcattt tactgaactt gataacgggc atgactttta
     1141 tgcaccacaa agctttttag cgaaggatgg tcggcgtatt gttatcggct ggatggatat
     1201 gtgggaatcg ccaatgccct caaaacgtga aggatgggca ggctgcatga cgctggcgcg
     1261 cgagctatca gagagcaatg gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt
     1321 acgccagcag catcaatctg tctctccccg cagaatcagc aataaatatg ttttgcagga
     1381 aaacgcgcaa gcagttgaga ttcagttgca gtgggcgctg aagaacagtg atgccgaaca
     1441 ttacggatta cagctcggca ctggaatgcg gctgtatatt gataaccaat ctgagcgact
     1501 tattttgtgg cggtattacc cacacgagaa tttagacggc taccgtagta ttcccctccc
     1561 gcagcgtgac acgctcgccc taaggatatt tatcgataca tcatccgtgg aagtatttat
     1621 taacgacggg gaagcggtga tgagtagtcg aatctatccg cagccagaag aacgggaact
     1681 gtcgctttat gcctcccacg gagtggctgt gctgcaacat ggagcactct ggctactggg
     1741 ttaacataat atcaggtgga acaacggatc aacagcgggc aagggatccg cgtcactctt
     1801 cccccttcac gaccttcaat aatatgcaat gcagcttccc gcccgataat gtcatgtgga
     1861 agctgaattg tggtcagcgg cggtaaaaac agatgcccga cgccaaccag attatcaaag
     1921 cccattacag cgacatcctg cgggatacgt acccccttcg ccaaaagaac ctgataagcc
     1981 acaaaggctg cgcgatcgtt accacatatc agaacatcaa aatctggttt gcccgatttg
     2041 aagtgagcat tgagtaaact tgcgagatcg gtgtagtgat catcacctgt tgccatgtga
     2101 aattgtttca cctcagccag atctcgtcca gcatcacgcc aagcctgctc aaatccctgc
     2161 cgacgatacc ctgttgccaa cgcactttcc ggtagccaga agcataatgg ttgacgatag
     2221 cccgccgcga gcaaatgctg tgttgattca tattgtgcag tgtaatcatc agggatataa
     2281 ctgggtaacg ctgggtcatc cgccacacag ttcgccaata caatattttc accatacaga
     2341 gactcaggca gcgtgatatg tcgcagcccc attgtagtat agataatgcc atccggacgg
     2401 tgggcaagca gctgacgtgc cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa
     2461 aaactattcc agccgaactc gctggcggtt tgctcaatgg caagcagaat atcaacagag
     2521 aaaggagtgg tagccgtgtc ctgcgccagc acggcgagag tcgacggctt acgtccttga
     2581 gcgcgcatct tacgggcgga aagatcagga acataattca gggtctggat tgcctgcaat
     2641 acgcggtcac gcgttgcagg acgcacagat tctgcattat gcatcacccg ggagactgtc
     2701 atcatcgaca ctcccgccag gcgtgcgaca tcctttaatg aagccatacc caagccgttt
     2761 gccgtaaaac gggcactgta gcagaaacag acgtcactgg cgagatccaa cgccctatca
     2821 cctgacacag caatacaata aaaaataaca ataattcccg gacaattgtc cccaattccg
     2881 cctctgttct cgccaacgag taaccatata ggcaagtaag taaaacatgc caactggcat
     2941 cgccaacctg gagaagaaaa tataaatatg gatcattgtg ctgccactgt atcccgacat
     3001 cagcccggaa attattgcga ttgctatcgg ttcaggtgca attggctgca cgatcgttac
     3061 ggactcgctt ttctggctgg tgaagcaata ttgcggcgct acgctcaatg aaacatttaa
     3121 atactatacg acagcgacat ttatcgcttc agtcgtcgct ctggcgggca cattcctgct
     3181 gtcatttatc atctaagcgc aaagagacgt actatggaaa acgctaaaat gaactcgctc
     3241 atcgcccagt atccgttggt aaaggatctg gttgctctta aagaaaccac ctggtttaat
     3301 cctagcacga cctcattggc tgaaggttta ccttatgttg gcctgaccga acaggatgtt
     3361 caggacgccc atgcgcgctt atcccgtttt gcaccctatc tggcaaaagc atttgctgaa
     3421 acagctgcca ctggggggat tattgaatca gaactggttg ccattccggc tatgcaaaaa
     3481 cggctggaaa aggaatatca acaaccgatc agcgggcaac tcctactgaa aaaagatagc
     3541 catttgccca tttccggctc tataaaagca cgcggcggga tttatgaagt cctggcacat
     3601 gcagaaaaac tggctctgga agcggggttg ctgacgcttg atgatgacta cagcaaactg
     3661 ctttctccgg agtttaaaca gttctttagc caatacagca ttgctgtggg ctcaaccgga
     3721 aatctggggt tatcaatcgg cattatgagc gcccgcattg gctttaaggt gacagttcat
     3781 atgtctgctg atgcccgggc atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc
     3841 gtggaatatg agcaagatta tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct
     3901 gacccgaact gtttctttat tgatgacgaa aattcccgca cgttgttcct tgggtattcc
     3961 gttgctggcc agcgtcttaa agcgcaattt gcccagcaag gccgtatcgt cgatgctgat
     4021 aaccctctgt ttgtctatct gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc
     4081 gggcttaaac tggcgtttgg cgatcatgtt cactgctttt ttgccgaacc aacgcactcc
     4141 ccttgtatgt tgttaggcgt ccatacagga ttacacgatc agatttctgt tcaggatatt
     4201 ggtatcgaca accttaccgc agcggatggc cttgcagttg gtcgcgcatc atgctttgtc
     4261 gggcgggcaa tggagcgtct gctggatggc ttctataccc ttagcgatca aaccatgtat
     4321 gacatgcttg gctggctggc gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg
     4381 ggtatggccg gacctcagcg cgtgtgtgca tcagtaagtt accaacagat gcacggtttc
     4441 agcgcagaac aattgcgtaa tgccactcat ctggtgtggg cgacgggagg tggaatggtg
     4501 ccggaagaag agatgaatca atatctggca aaaggccgtt aataacgttt caacgcaaca
     4561 tcgcaatcct ttccctgggt gagcgatgct gccgatggcg cagacttaag atccccggtc
     4621 ttacccgcta taaccccctc cttttatcat cacccaacgc ctttcgctgt aaacggcggt
     4681 ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa tagccgccat acggaaaatc
     4741 tcatttgcag aaatagaaag tgactgctgg gttatttcat tattaatttc attaagaact
     4801 cctgaaagcg aaccatagta tttatccata atttgtgacg atgaattaaa cacggggtta
     4861 aattgatcga tggttgctgt caactgacta tggtgtaacg attcgcgtcg tccccacagc
     4921 gtcattgtca acgacgtacc aactgatcct gacaaggtac gaaaaaaatt actcatactc
     4981 gaggcattgg caaatttatt atctggcaag cctgaaaacg aaatcgttgt taagggtaaa
     5041 aagaaacagg caacggcgaa tccctggaaa aactgtggca aaatgatgcc tgtaaaatca
     5101 atcgttggca taaatgtcac agaacgccag taatagcaaa ccgcatacat caaaaaactg
     5161 aatgtcacta acacccgcat gtctatttta ttgccataac gtcctatcaa aggtgaaatc
     5221 aatagtggca tgatgccgat gggcgcataa gcaagcccgg cccatatcgc attatacccc
     5281 atcgttttct ggagtaactg cggcataagg acgatcgctc cagagtaaaa taaatacgcg
     5341 caggtgatac tcacaatacc aatggtgaaa ttacgggact taaacaaact gagatcaaga
     5401 atcgggttct ctgaggtcga ttcccaaatg actaaagaga tcagagaaat aactgatact
     5461 actgttaata ttattattgt actcgagttg aaccaatcca gatcgcgccc tttatcaagc
     5521 ataatttgca agccaccaac accgagcact aacagggtca gtcttggtag attcattttt
     5581 accggtgaag tttcagtttc tcttccttta agtaaggtta agcataatgt caggacgata
     5641 atccccatag ggacattgat taaaaatatc caaccccagc taaagttatc gcaaatatag
     5701 ccgcccaata tcggcccaca tatcggagcg ataatcacgg tcattgacca taatgccaga
     5761 gcaaatgttc ttttttctgg tggataattc cttaataata aactctgtga cagtggaatt
     5821 aacggccccg ccattaatcc ctgaacgact ctaaaaaata tcagcacatc aagattggtc
     5881 gataaactac acattaagga agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt
     5941 aattcgccta ttctttgtgc cagcctgccg gtaacaggga tcgcaatggc atttgctacg
     6001 ccaaacgagg tgataaccca ggtgccttcg tctgttgatg ctcccagaaa gccagatatt
     6061 gtcggtattg cgacgttaga aatagtggaa tccaacattt gcataaacgt cgctaatgac
     6121 aatgcaatag taacgcacca taacgtccca ccggttaatg gtgccggagt tgatttagtg
     6181 attgccatta aagttgtcca ttatgcgaaa taatgttgct aatttctttt tctatcggac
     6241 tggtatcgat aactaaagcc ttactggtat aagcaggcat ggaggtcacg gttgaagcca
     6301 gatcaggcat ctcagcaatg tcttcgtcct tcgtatcaat agttgcagtc atcgataaac
     6361 caatgcgcaa ggggtgttcc atgagttctt ttggatcaag agaaacttca accggtacac
     6421 gctgaacgat tttgatccag ttccctgtcg cattttgtgc aggtaataag gagaacgcat
     6481 tgccggttcc catattgatc cctgtcaccc gaccatgaaa cacaacattt tcaccataaa
     6541 gatcgctgat aatattgact gattgaccaa tccgtacatc cgtgagttgt gtttctttaa
     6601 agttggcatt aacccacatt tgacgtgccg gtacgacagc cattaacgat tgtccggaac
     6661 tcactgtttc gccgacctga acacttcgct gggcaatata gccagtaacc ggactcctta
     6721 tatccgtacg tttaagcacc aaccaggctt ctttggttgc atccgccgct tcaactactt
     6781 gtggctgacg gtttaatggt gtgttcatta ctaaagcttt attcgcttta taagcctgga
     6841 tagcggcatt cagtgccgct ttgctactta ttaacgtatc tttggtatgc tccagcgttt
     6901 cttttgaaat aaccccctgc ttcgctaaag gcacacgacg gttataatct tctaaagatt
     6961 gttgatactg aatacgtgct gaagcgactt cggcactgta ttgtttatcc tgtaagtaca
     7021 gtttattcgt ttgccgaaca atatttgcca gattattttt agctttattg agtgcgatag
     7081 tggcatcagt tttatccagt gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt
     7141 tatgattaac tacagtgaca ctacctgaga cttgtgcaga aattggatct gcattccccg
     7201 tgacataggc gtcatctgta ctaatcatgt cttctaattc cattgaccaa taggcatagg
     7261 cacctgaaaa cgcaataaat aaaactatca ccaataaaga aaagtatttt tttctgttag
     7321 actgtttttt atttgaatta atctgttcca ctattatctc tcatttcgca tagatgaaat
     7381 ttatgaattg aatgaaaaga gtattcattc ttcatatcaa acacagtgca tgtgttgcac
     7441 attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac aacagaggac
     7501 taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtgaga agacttatag
     7561 tgctaacttg aaactataaa tcatcggtat aatccctgat tttattgttg acatttcatt
     7621 tatgccgact atttatatgg tatacttgtc gaattatctt aaaggaaact cagattttct
     7681 tatttttatt gagaaaatga gatgacgcct tatgtctgta ttactacagg gagaagggag
     7741 atgcttcatt gcaaagggaa taatctatga acgcaataat tattgatgac catcctcttg
     7801 ctatcgcagc aattcgtaat ttattgatca aaaacgatat tgaaatctta gcggagttaa
     7861 ctgaaggcgg aagcgccgtt cagcgggtgg aaacacttaa gcctgatata gtcatcattg
     7921 atgtcgatat ccccggagtt aacggtatcc aagtgttaga aacgctgagg aagcgccaat
     7981 atagcggaat tattattatc gtctccgcta aaaatgacca tttttacggg aaacattgtg
     8041 ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg catgaacaat atcattgcgg
     8101 ctattgaagc tgcaaaaaat ggctactgct atttcccctt ctctctcaac cggtttgttg
     8161 gaagtttaac gtccgaccag caaaaactcg actccttatc gaaacaagaa attagtgtca
     8221 tgcggtatat tcttgatggc aaggataata atgacattgc ggaaaaaatg ttcatcagca
     8281 acaaaactgt cagcacgtat aaaagtcgcc tgatggaaaa attagaatgt aaatcactga
     8341 tggatcttta cacattcgcg caacgtaaca aaatcggcta atcacatgaa gtttttaccc
     8401 tatttttttc ttctctgttg tggtctttgg tcgaccataa gtttcgcaga cgaagattac
     8461 atcgaatatc gtggcatcag tagtaacaac cgtgtcacac ttgatccagt acgtctgagc
     8521 aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc
     8581 caaacggcta ccttgttgca taccgattcg cagcaacagg ttcgtggtat taatgctgat
     8641 tatttaaatc ttttaaaaag agcgttaaat atcaaattaa cactccggga atacgcagat
     8701 catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt atcacattta
     8761 gttacttcgc cgcctcttaa taatgacatt gctgcaacca aaccattgat aattaccttt
     8821 ccggcgctgg taaccaccct tcacgactca atgcgaccgc ttacctcacc aaaaccagta
     8881 aatattgctc gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc atttccaaaa
     8941 gcaacaatta tctcttttac aaatttatat caggcattag catccgtctc agctgggcac
     9001 aatgattact ttattggtag taacatcatt accagcagta tgatttcccg ctatttcact
     9061 cactccttaa atgtagtgaa atattataac tcgccgcgtc aatataattt tttcttgacc
     9121 agaaaagaat ctgtcattct taatgaagta ctcaatagat ttgttgatgc tttaacaaat
     9181 gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc ctttctgaac
     9241 aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc caatttaaag
     9301 gtgctggaaa atccttactc gcccccctat tctatgacgg atgaaaatgg ctcggttcgg
     9361 ggcgttatgg gggacattct taatattatt accttgcaaa caggtttaaa tttttctccg
     9421 atcaccgttt cacacaatat ccatgctgga acacaactta gccccggagg atgggatata
     9481 atacctggcg ctatttatag tgaagatcga gaaaataatg ttttatttgc tgaagccttc
     9541 ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa
     9601 aaaggaatga aagttgccat tccatattat tatgagctgc attcgcaatt aaaagagatg
     9661 tatccggagg ttgaatggat acaggtcgat aatgccagcg ctgcatttca caaggttaag
     9721 gaaggtgaac ttgatgctct ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat
     9781 tactatccta atgaacttta tcattttctt attcctggcg ttccgaatgc atcgctttcg
     9841 ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc actgaatgca
     9901 attcccccaa gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc
     9961 attgacacat gggacctata tagcgagcaa ttttatattg ttacgacatt atccgtttta
    10021 ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg tcgtcgtaaa
    10081 gtcattcagg gtgatttaga aaaccaaata tcattccgaa aagcactctc ggattcctta
    10141 ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcataa tagtgctttt
    10201 gaacattatt tcactgcgga ttactacaaa aatgcaatgt taccattaga aaacagtgat
    10261 tcacccttta aagatgtttt ttctaatgcg catgaagtca cagcagaaac gaaagaaaat
    10321 cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag atgcattaat
    10381 cactggcata cattatgcaa tcttcctgca agtgacaatg cagtatatat ttgtggttgg
    10441 caagatatta ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa aaataaagcg
    10501 ataaaggcta ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga aataagaaca
    10561 ccaataagct ctattatggg cttcctggaa cttctgtcgg gttctggtct tagcaaggag
    10621 caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct cggcttaatt
    10681 ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca accacaatgg
    10741 gtcgatatcc ctactttagt ccagaacact tgtcactctt tcggtgcgat tgctgcaagc
    10801 aaatcgatcg cattaagttg cagcagtaca tttcctgatc attacctggt taagatcgac
    10861 cctcaggcgt ttaagcaggt cttatcaaat ttactgagta atgctctcaa atttaccacc
    10921 gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca cgctgttatc
    10981 aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca acaactgttt
    11041 aagcgctaca gccaaacaag tgcaggtcgt cagcaaacag gttctggttt aggcttaatg
    11101 atctgcaaag aattaattaa aaatatgcag ggcgatttat cattagaaag tcatccaggc
    11161 ataggaacaa catttacgat cacaatcccg gtagaaatta gccagcaagt ggcgactgtc
    11221 gaggcaaaag cagaacaacc catcacacta cctgaaaagt tgagcatatt aatcgcggat
    11281 gatcatccga ccaacaggct attactcaaa cgccagctaa atctattagg atatgatgtt
    11341 gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg
    11401 cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg caaactccgt
    11461 gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc taacgaacgt
    11521 gaaaaagggt taagttgcgg catgaactta tgtttgttca aaccgttgac cctggatgta
    11581 ctgaaaacac atttaagtca gttacaccag gttgcgcata ttgcacctca gtatcgccac
    11641 cttgatatcg aagccctgaa aaataatacg gcgaatgatc tacaactgat gcaggagatt
    11701 ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca tgcactagaa
    11761 gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc ggctaacatc
    11821 ctgaatttgc aaaagttgat taatattagc catcagttag aaataacacc tgcttcagat
    11881 gacagtaagc ctgaaattct tcagctgctg aactctgtta aagaacacat tgcagagctg
    11941 gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc
    12001 taatgtggga gctatttacc agcacatctt acatttatga tgagaactcc tggcgaattt
    12061 gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc gcgcagccgc
    12121 tgatttttat cggattaccc ggcatcatta ttcctccggc ttcaatcaac atatttctcg
    12181 cctgagtttg tggcaaatta atggcctcag ccacacttaa taacggcgcg acgggcacac
    12241 caacttcatg tattttggct aaccaaactt cagctgcctg cgtttttaac gttcgctcaa
    12301 tatattgttt aagaatagcc tggttttgta cgcgtaaaat attgctgcta aatcggggat
    12361 cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc ttgtcattac
    12421 cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg
    12481 gatggcgatt tcccaggcgt tgtggtgact tccctgtcgc aatatatgcc atcagaccat
    12541 gctccagaaa actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc cctctctggc
    12601 tcttttcgcg gccataaagt gcactcacta ttccgctgaa taaatagaca ccgccgcata
    12661 gatccgcaag agaggtacca acacgcactg gcggagcatc agggtaccct gtttccatca
    12721 ttatcccact cattgcctga atgatggtat cgtaggcagg agcatctttt agcggaccgg
    12781 tatgtccgaa acctgacgat gaagcatata tgaggcgcgg gttgatttct tgtaaccttt
    12841 cccatgaaaa ccccagtttt tccattgtac ctaggcgaaa attctcagct aatacatcag
    12901 cttgtttgag catatttata aatatacttt tatcgtgatc attctttaaa tcaagaacca
    12961 cactctcttt gccatgatta ataaaactgt aatagagtga ctgtccatcc acatagggac
    13021 caaatgtgcg ggtatcatca ccatgacctg gtggctcaac tttaattacc cttgcgccca
    13081 tattataaag aagttgagtt ccgaaaggtc cattaaggac atgtgtcata tcgataacta
    13141 ataagccttc aaacggccct ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa
    13201 gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat taattatgaa
    13261 accagtcgac tgacataaat ccacaaggga gccgtgacga caaagcccag tacgctcaca
    13321 gccaatgacg ctgtaccagt acgggtgtag acattaaacc ggctggcaat aatgattccg
    13381 gagaatgccg gcggtaatgc gcctgccagt accatcatct gcaaatgttc gctgttcaaa
    13441 tgacatgcca taccaacgag aagcagcgcc agtggcatca gaatcaactt caggaaggtg
    13501 ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg
    13561 aataccgcta cccctgagtt agccttcgca atcagattaa aggttgggtc ccatgctgcc
    13621 ggaattttta ccccaaccag caccaggatc gttgccagaa caggtgccca tactactggc
    13681 tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc cgctcctgag
    13741 gaaggattca gcaaatacag accaatagga atagtaattg cgttaacaat aatagaaata
    13801 attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag aactgcaaac
    13861 cccaggaatc caatggtagg tgaacccgca attaatgcac atacagctgc ttctgcatgg
    13921 gtacgtttaa aaaatttgta gcaaccaaac caggagaaaa agaaacatcc gacaataacc
    13981 acaagtgata caagggtcag acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta
    14041 atagaaacaa atagagccgc aggaagagcg tagtttaata ccagtttatt aaatgcgcgg
    14101 gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat aacccaacag catgattacg
    14161 ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc gataagggtc
    14221 ccctcaccat cgcataatga gttggcaaga ggagacgatg tcagtattta attaccagcg
    14281 acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc agggtcgata
    14341 accacattaa taatggtcgg tttgcgcgac tgaataccgg tggttaaagc atgacgaagt
    14401 tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg catccattaa tttgtcatac
    14461 cttgcatggt gcaacagatc cgttggtgat ggtgcaccag cgccactgag atcaacaccg
    14521 tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag gttatatcga
    14581 caaatcgttt caatttccat cccactgaaa ccaaaagcac tatcaccttc aatggcgaca
    14641 accggagaac cagaagtcac gctagcaccg atggcatagc ccataccgat gcccatgaca
    14701 ccccaggtac cacaatccag acgacgacgt ggtttataca tatcaataat atttcgtgca
    14761 ttatccaggg tatttgcacc ttcattaact aaataaatat cctggttctc gcgcaatacg
    14821 tcgcgcacag cacttaatgc attaaagtaa tttaatggtt gagtatctgt acttaatttt
    14881 tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg ccataccagt
    14941 ggagtcgtaa atgtgttttg tttcagttct gccagcatac cttgcatact ggatgcaata
    15001 tcaccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc
    15061 agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccaa taaccaattc
    15121 agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga acgcgcagct
    15181 gccgcagaaa gtggatgcgt atcttcaagg atccctttcg ccatagacat tggcaggaat
    15241 ggaatctgag cactttcaat aaattcacga agctgttcat cagcttgtga atacgccgcg
    15301 cctttgccaa ggataattaa tggccgttca gcttttgcta aaagcgaaat tgcgctagtg
    15361 actgacttcg ggcatggcaa taatgctggc gacggatttt caactttaac aatcgtggtt
    15421 aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc aagataaact
    15481 ccgccagggc gacccgatac agagacccgg atagcgcgtg ccaatgcaat gccaagatcc
    15541 tgcggctgat taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt catttggtcc
    15601 agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc
    15661 atgataatcg gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc attgaggaat
    15721 cctggcgcag aaactgtcag gcagatcccc ggtttttggg taagaaaacc gcttgccgca
    15781 gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg
    15841 gcatggcgtg ccatatccgt cacaggaata cctacaacac cataaatagt gtcaatatta
    15901 ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag ttgatctgac
    15961 atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt taatatcaga
    16021 tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc tcctgcaata
    16081 cagcagcggt atgttcacct aatagcggcg cagctttaat atccggcgta aaggcagaga
    16141 atttcattgg acaaccaacg gtcagatatt ttccacgcaa cggttgttcc acttcaacaa
    16201 cactgccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt aaaaccggtg
    16261 cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca atagtgacag
    16321 tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac
    16381 tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg ttttcccagt
    16441 tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag cctttacatt
    16501 tcaggatcca gccaggctga ccgccaccac ctgcattacc accgcgggga actgcatcac
    16561 caaatgtacc attcggatac tgcgggtatt cttccagata acccaattta tcgagacgct
    16621 gctggtcacg taatttcacg cggcaaaggt tcaatacggc atcctgcatt gacatggtga
    16681 ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca
    16741 aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc ggaccatccc
    16801 aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc aacgttttca taggctttta
    16861 cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc agacgtggat
    16921 tgatttcttg aatatgctcc caggtgaagc ccatgtgatc aatggcccct ggatgaaagt
    16981 tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg ccttccgctg
    17041 ttttggtatt taactcaata gaacgtttgt tactgttaag catggtgaag taaagcgcat
    17101 cgatatcagg aatatctcgc agttggtgac gcgttacgtc accaacgccg ggacgttcaa
    17161 ttttaataac gtcagcgcca aaccaggcca gcatttgagt acaagatggg ccagattgca
    17221 caccggtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca atacctatta
    17281 gttaattaaa tatgtgttaa tttagcgttt atttaagtca taacgccctc tataaataat
    17341 ctgcaaaata tcttcgtagc ggcactcgat atttttaaat tcctgtatca agtactgaaa
    17401 attattttat cgccacttcc aattcctttc tgcatggtaa gtagtgagga cgaaaagaag
    17461 aatatgataa atattgattc gtggtaatca gagagagtcg cgacatttgt gatgttattc
    17521 ttacaactgt aagaagcgcc ggtgatttac aagtaagagt atgattacgc tttttaaagc
    17581 gtaccataaa gcataacata ataaatgatt tgtgatgaca cgggattatt gcggtttact
    17641 ttgaggaaaa taaaatgcgt gtttatagaa ccgcgagcgc caaataattt tatgaccaca
    17701 acctgtcgta ttgtggtcat aaaaaattgt cggctggagg gagttaaatc ctttacctca
    17761 atatactgcc tgtcagcatt agttcacaaa cagtgcttcg ctatcgacga tgattccatc
    17821 ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa gcagtttctg
    17881 cgcatcagca agtgcattac gagtctgttt aagcggcatc agatattggt tttccattac
    17941 gccaacaccg gccaggcgaa gctcttccat tgcgcccttt ttatcccctt tcgccatttt
    18001 ttcattggca atttttatcg cggcttcttt ttcgggtgtt gctacataac tctcagaaat
    18061 tccgacagat gcattaatga caatatattg gtcatcattg agattggtct ttttacccgg
    18121 cttagcgaac tttgcccatt cggtactgtc atctgacagc aaagcggaag cttcattggt
    18181 cagttctttt gccttttccg gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc
    18241 acgcatcgca tataaacctt gttcagagat acgctctgct gctgccgttt tctgggtttg
    18301 ctgagtttgc tgttgtgccg ctaccggtgc attatcagca gcccatacag ttgaagatgc
    18361 cagaattgct gtgaccatcg tagccataat taaacgtttc atacttacct cattctttaa
    18421 cagttttaga aaacgccgcc atcatattgg gtgagcatga aatgagaaat cagagagcaa
    18481 cggtcatttt gaaagggtta tcttacaatt gtagttatta tcgcaaagcg gcatttagtc
    18541 ttacagttgt aggaatatgc ctcctgtcgc agagaactat tcttatatac cacaaagaga
    18601 tgtcatttat tatattgatg taaatattga ttaattatat gtaaatacag ccataacatc
    18661 gtatggttct cagtgaacca ttttcaggat aactttctat gaaagtaaac ttaatacttt
    18721 tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg
    18781 gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac
    18841 actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgtgccac
    18901 cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc
    18961 atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga
    19021 acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg
    19081 aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacca gttcattacc
    19141 gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa
    19201 agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat
    19261 acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa
    19321 cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa
    19381 gcatcaatga aaacagcacg ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt
    19441 gcacgattaa taatcagtag ctgaaagcag tcagccagtg ataaagtaga gccaccgcat
    19501 tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg
    19561 tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa
    19621 caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct
    19681 acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct
    19741 tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct
    19801 gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc
    19861 ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga
    19921 aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg
    19981 gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca
    20041 ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac
    20101 ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc
    20161 taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg
    20221 tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc
    20281 gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc
    20341 tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt
    20401 gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcttatat
    20461 gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg
    20521 tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac
    20581 tctttccctc ctcaaatcgg ccatgataac ctcatgattt ttatagcttt ttcttaagtt
    20641 ttttccttcg ctgaatatct aattattacc gtcagaaatt aaactgtcgc cgatatgaga
    20701 cacttgtaag ttactaaaag taaataagaa aatgcctctt gtctcgtttc agttttaggc
    20761 gtacattagc gccgtctcga gcagtttagc tcagaattct ggggtgatgc ccacggtaaa
    20821 atacagggct tttaaggtcg ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag
    20881 tcattcaggc gtaacagtaa ttacgcggag agatgtaaag tgaaatattt ctttatgggc
    20941 atttctttta tggtcatcgt ttgggccggt acttttgccc tgatgatcta aaagcaaact
    21001 gtgcaatgaa aaacaggagc catcaggctc ctgtttgcat ttctgacgac ggtgtttatt
    21061 ccgcgttttc gtgaatatgt ttgctgctga cgggtagtaa accgtcggca cggaacatcg
    21121 ctttaatccc tctgatggcc tgacgaatac ggtcgtggtt ttcaatcagt gcaaagcgaa
    21181 catgcgtatc accatagtcg ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt
    21241 taaggagctt cttggcaaat tccagcgatc ccatggccgc atatggttcc gggattttcg
    21301 cccagacata catcgaagcc ttcggcattt cgaccatcca gcccgcttca tgcagccctt
    21361 taaccaatac atcacgacgg cgtttgtact gttcggcaat gtcgcgcacg cactgttgat
    21421 cgccctcgag tgccgcaatc gccgccacct gcaacggcgt aaaagtgccg taatcgtgat
    21481 agcttttgat tcgcgccagg gcactgacca gcgttttgtt gccgaccata aagccgatac
    21541 gccagcccgc catgttgtag cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg
    21601 cacccggcac ctgcataatt gacggcgctt tccagccatc gtagacgata tcggcatagg
    21661 ccaggtcatg gaccaccagc acatcgtagc atttcgccag cgccacaacc ttttcgaaga
    21721 actccagttc cacgcattgc gcggttgggt tcgacgggaa gccgaggatc atcatcttcg
    21781 gtttcggata actttcacga atggcgcgct ccagttcgtt gaagaaatcg acaccttcca
    21841 ccagcggcac tgagcgtacc tgcgccccgg caatcaccgc gccataaata tggatcgggt
    21901 aacttggatt cggcaccagc accgtgtcac catgatccag cgtcgccagc atcagatgcg
    21961 ccaggccctc tttcgaacca atagtgacga tggcttctga ttccgggtcg atttcaacgt
    22021 cgtagcgatc ctgataccag cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag
    22081 tggagtaacc atgcgtgtcc gggcgctggg ccacagtaca taatttttcg acgatatgcg
    22141 gcggagtcgc accgtccggg ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac
    22201 gcgcagccat tttcagttca gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc
    22261 gcgtaaagcg acgttcaggg cgagtgtcag ccataatttt ctcagattaa cgttagcgcc
    22321 cggaccgtcc gagcgacgct gccacaatgg tggctgtttt gaaaatagcc tgattaattt
    22381 ctgtctgtcc agcgttttag aacattttta tcaatggatt taaatgagga actccacttc
    22441 aaaaacagga aatttttcta cgctaacaag aaaatcacca ccaattttat taacattata
    22501 atttcaataa atttaccagc tagaagtgca ttatcaagag atacattgct aatacaccat
    22561 ttatctttca atcccctttt aaatttatgg tttttcctca aaaacggcct gctggtcatc
    22621 agccttcagg ttacctatca tagaggttta atccttattc agagtcaccc gtgcacgaaa
    22681 tattcaacat gctgctggcg gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct
    22741 ttctcatccg tatccgcctg tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag
    22801 aattgctcgc cgttaccgcc attttctcgc tgttcgccct gttcagcacc tggtccggcg
    22861 ttcccgtaga aggctcgctg gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc
    22921 tgtttggccc gtgggtaggc atcattaccg gcgtgattgc gggtattcac cggtatttaa
    22981 ttgatatcgg cggcgtgacg gcgatcccct gctttatcac cagcattctg gcgggttgta
    23041 tatcaggctg gataaacctg aaaatcccca aagcacagcg ctggcgctgg atatcgccaa
    23101 caaaacgcta ccgctgtttc gccatgtcaa tagcgagtca ttacgcaagg tctgcgaaat
    23161 tatccgcgat gacattcacg ccgatgcggt ggcgattact aataccgatc atgtgctggc
    23221 ctatgttggc gtgggtgaac ataactatca gaatggcgat gacttcatta gcccgactac
    23281 ccgtcaggcg atgaattacg gaaaaatcat cattaaaaac aatgatgaag cccaccgcac
    23341 accagagatt cattccatgc tggtgatccc attgtgggag aaaggggtcg tgaccggaac
    23401 gctgaaaatt tactactgcc acgcgcatca gatcacctcg tcattacagg aaatggcggt
    23461 cggtctgtcg caaatcatct ccacgcaact ggaggtttca cgcgccgagc agctacgtga
    23521 aatggcaaat aaggcagagc ttcgcgccct gcaaagcaaa attaatcccc attttctgtt
    23581 taacgctctg aacgctattt catcgtcaat ccgtctgaat ccggataccg ctcgccagtt
    23641 gatctttaat ctgtcgcgtt atctgcgcta taacattgaa ttaaaagacg atgagcaaat
    23701 cgatatcaaa aaagagctgt atcaaattaa agactatatt gccattgagc aggcccgctt
    23761 tggcgacaag ctgacggtta tctatgatat tgatgaagag gtgaattgct gcattcccag
    23821 cctgctgatc cagccgttgg tggagaacgc cattgtccac ggtattcagc cttgcaaagg
    23881 taaaggcgtt gtcaccatca gcgttgcaga gtgcggaaat cgggtacgca ttgcggtgcg
    23941 agataccggg cacggcatcg ttccaaaggt gattgagcgg gtcgaagcga atgaaatgcc
    24001 gggcaataaa attggcctgc tgaatgtcca tcatcgcgtg aagttattgt atggcgaggg
    24061 gctgcatatc cgccgcctgg agccggggac ggaaattgcg ttttacattc ctaaccaacg
    24121 caccccagtc gcctcacagg ctacgttatt gctttgagcc ggagtgatat tgtgaaagtc
    24181 atcattgttg aagacgaatt cctggcacaa caggaactga gctggctaat taaagagcac
    24241 agccagatgg agattgtcgg cacctttgac gacggtctgg acgtgttgaa gtttttgcag
    24301 cataaccgcg tcgacgccat ttttctggat atcaatattc cgtcgctgga tggcgtgttg
    24361 ctggcgcaaa acatcagcca gttcgcccat aaaccgttta ttgtgttcat caccgcgtgg
    24421 aaagaacatg cggtagaagc gtttgaactg gaggcgtttg actacattct caaaccgtat
    24481 caggagtcac gtattaccgg gatgctgcaa aaactggaag cggcctggca acaacagcag
    24541 accagcagta cgcctgccgc gacggtaacg cgtgagaatg acaccattaa tctggtgaaa
    24601 gatgagcgaa taatcgtcac gccaattaac gatatctatt acgccgaagc gcacgagaaa
    24661 atgacctttg tctatacgcg gcgtgaatcc tacgtaatgc cgatgaacat taccgaattt
    24721 tgcagcaaac tgccgccgtc gcattttttc cgctgccatc gctcattttg tgtcaatctg
    24781 aacaaaatac gcgaaatcga accgtggttt aataacacct acattctgcg actgaaagat
    24841 ctggattttg aagtgccggt cagccgcagc aaagtgaaag aatttcgcca gttaatgcat
    24901 ctttaatcgg gaactttcat gaaagcacca ggcttgcccg ccgatcaaca atttttcgcc
    24961 gatctgttca gcggcctagt gcttaacccg caactactgg ggcgcgtctg gtttgccagc
    25021 cagcctgcct cgttaccggt gggcagttta tgtattgatt ttccccgtct ggatatcgtg
    25081 ctgcgcggcg aatacggcaa cctgctggaa gcaaagcagc agcgaatggt ggaaggagaa
    25141 atgctgttta ttccggcacg cgcggctaat ttaccgatca acaacaaacc ggtgatgctg
    25201 ttaagcctgg tgttcgcacc gacctggctt gggttatcgt tttacgatag ccgcaccacg
    25261 tcgttgttgc atcctgctcg ccagacccag cttcccagcc tgcaacgcgg tgaaggtgaa
    25321 gcgatgctta ccgccctcac ccatcttagc cgttcgccgc tggagcagaa tatcattcag
    25381 ccactggtgt taagtttgct gcatctttgc cgcaacgtgg tgaatatgcc gccgggtaat
    25441 tcacaaccgc gcggcgattt tctctatcac agcatttgta actgggttca ggataattat
    25501 gcccagccgc tcacccgcga gagcgtggcg cagtttttta atatcacgcc caatcatctg
    25561 tcaaaactgt ttgctcagca tggaacgatg cgttttatcg agtatgtgcg ttgggtgcga
    25621 atggcgaagg cgaggatgat tttgcagaaa tatcatctgt caattcatga agtggcacag
    25681 cgttgcggtt ttccggatag cgactatttt tgtcgcgttt tccggcgtca gtttagtctg
    25741 acgccgggag agtacagcgc ccgttttcag ggctaacgtc agaaggttaa ttctgtttcc
    25801 agcagcgtca ggatattttg tgcatcttgc gcggcaaaca acgactggcg gaagttttta
    25861 ttcaccagtt tacgcgccag ctgcgagaag actttcacat gattcatccc ttcgttagca
    25921 cccagcgtca gcatgatcac cagttcgact tcgcccattt ctgactgcca gtcaaccggt
    25981 ttcgccagcc gggcaatgct gatactggaa tgacggatcc actgagattt ggtgtgcggg
    26041 atcgctacgc caaaaccaac gccggtggta acaatctctt cccgctgcca gacatcttct
    26101 tccagctcaa acggatgttc agtgcgcccg ttaacgccga ggttgccgca caggaactgg
    26161 atcgcctgct ctttattgct aaaatcctga tcaacaaaga tattctccag cgccagcagt
    26221 gggcgaacgt cttcttccgg cgtaaaggcg gtgagtaacg cttcaatttc ctgcgcactg
    26281 cggcattcac atgcctgacg cgccagttcc cgacacgcct cgctatccag ttgacgaagc
    26341 tggcttttca ccgccggaat acgcgggcta ctcatactca gctcatccag gcccagccca
    26401 agcagtagcg gcagataacg gctttcaccg cccagttcac cgcaaatgcc tacccatttg
    26461 ccccgctgat gcgcagtggt aactatttgc tgcaacatgc gcaggaacga tggcgtaatc
    26521 gggttatata gcggcgatac gcgcgggtta ttacgatcga ccgcatacag atactgggtc
    26581 atatcgttgg agccgatact gaagaaatcg acctcatcgc agaagtggtc gatgatgtag
    26641 cacaccgacg gcacttccac catgatccca agcgtaatcg tctctgcatg acgcaggcca
    26701 tcgcgcttaa gctcaacgat cgctttttgg atctcgcctt tcacccataa gatctgatcg
    26761 aggctgtgaa ccatcgggat catcaactgg gcgttgccga aactggcggc gcgcaaaatg
    26821 gcccgcagtt gagtgcggaa caggccagca aattccgggt aaatacgtac cgcgcgatag
    26881 ccgaggaacg ggttctcttc ctggggaata ttcagataag gaatgctttt atcgccgccg
    26941 atgtccatcg tgcggaagat aatcggcttg tcgcccgccg ccagtagcac ctgctggtag
    27001 gcttcaaatt gctcctgctc gtccggcgcg ctgtcgcgat ccatatagag catttcggta
    27061 cggaacagcc cgacaccttc cgcgccgttg gcaaacgcgc ctggcgcttc cagagcggtg
    27121 ccgatattcg ccgcaatatc aatacgcttg ttatcacggg aataggccag ctgcgcggca
    27181 gcctgcgcct gttgtttttg gcgtttatcc gccagcgtct gcgcgacctg ataataaccg
    27241 ctcaccgcgt cattcgggtt aatcgccagc acgccgcact gggcgtcaag cactgcaggt
    27301 tgcccggcat aacgggcaat cgcatccagc ggcaagccac tcagtaccgg gatcgccgag
    27361 gcacgggcca gaatcagtgt atgcgaggtg cgcccggttt tctccagaat catacccgca
    27421 agatttttca aatcaaggct caaaaactgg cttggggtta aatcttcagc caccagaatg
    27481 gtcggttttt ccagcaccag attgttgcgc ggcttcagtt ccggccaggt gatatgcagc
    27541 aactgttcgc tgatatcgcg aatgtcgctg acccgctcgc gcagataatc gctggcagaa
    27601 gcagatagtt tggcgcaaac ctgctccata ttgctgatga tcgccgcccc cagtccctga
    27661 tgctgttctg tcatcaggcg acggatattg cctgcaaatt catcatcctg aatcagcgac
    27721 aaatgagcgc tgaggatagt tttgctttcg ccgtcacgct cacgcaattg ctgattcagt
    27781 tgctcggcaa gcgttgccag gctgtgctcc agccgggtgg aatcttgcgc actggcgggg
    27841 attgcccgat aactgtcgag gctgtcgctt tgtaacaggg tcagcgtacc cacgccgacg
    27901 ccgcttgcca gcacattgcc gtacagtaaa tccgggttaa ggcggcttaa tgaacgcggc
    27961 agcggatgcg ccgtcagttc tgcctgcgtc ggctgaacgc tgtcgctgtc gataaagcgc
    28021 accaggatgt actcttccag cacgcgccgc gcctgctctt catcgctgcc gctaatgttc
    28081 aggctgcaac tgtcattaaa taaggtgcca gttccaatca acgccagcga gcttttagca
    28141 tcagcctttg cgttctggcg atggttgata aaggtgattt cgctttgcca ctggctgcac
    28201 tgttctttaa gttcccacgc cggacgagca tgtagaccgt taggcaaagg acagagaaat
    28261 tgaatcgtta acataaccgc tccgggttaa tcaggagatc atctgaaatc cgtcagttga
    28321 acaaccgtct cacgcgtaag acgttgaata agggcagata aaagttgctc catctgcaaa
    28381 atgtcgcggc aatcggcaat cgatgccgcg caatgtccgt ggcgggtggc aggccctatt
    28441 accaatgtgg gtacgccagt accggttaag tgcaccgctc caccgtccgt gccgccgtta
    28501 ctgaacatat ccgcctgcaa cggaacgcca atttctgccg ccacggtttc aatccacgcg
    28561 gtaagttttg gcggcgcaat cagcgattta tcgcttaaca ccagcatcgg cccgttacca
    28621 atctgacgat ggttagccgc gccataatca aagtttttcg cccagcaggc ggtatcaagc
    28681 acaatggcga cgtccggcga caccgcgcgg gtagcagttt gcccgccgcg taatcccacc
    28741 tcttcgctgg aacttgccac cagccacact tccgcaggca gttcggcgtc gtgcagctca
    28801 cgtagtaacg tcaccagcag atagcaactg aggcgatcat caaaggcttt gcccatcacc
    28861 cgctggtgag ggaggacctg aaaagtggta tcgaaggtga cgcgatcgcc tggacgaatt
    28921 cccgcctgca tcacttcgtc ataggtgcgc gcgccaatgt ccacgcgcat ggcgctaacg
    28981 tcattcccct gccggtcgcc gtcaagcagg cctggaattt tgcactcttc acgggtggtg
    29041 atgcgcaccg gctgcagctg gcgggcagcc atgcgcacgt tgccaaccgg cagcacgtca
    29101 atagcgcctt cgcgggagat gctgcgcacc ataaatccca cttcgtccat atgggcgcag
    29161 atcatcacct tcggacctgt cgattcattg aggcggatca gcaccgatcc cagaccatca
    29221 aatcgcactt ctttttgcag gcgagccgct tcttccagca ggatctgccg cacttcctgt
    29281 tccgaggagg cgatcgcatc tgcctcgctc aacgctttta atagcgataa atccattatg
    29341 cctctcccgt gagcaacact gttttcggca tggcgtagag cacttctgcg ccttgcgggg
    29401 taaccagcac aacatcttcg atgcgcacgc ccccttgccc tagcaaataa atccccggct
    29461 ccacggtcag caacatgcct ggctgtagcg tcgtggtgtc ccgcggtgaa aaacgcggat
    29521 cttcatggac ttcaatgccg atagcgtgac cagtgttatg accgaaatag tcgccaaatc
    29581 cagcctcggt aatgacccgg cgcgcggcgt cgtcaatctg ctggcagcgc acgccggggc
    29641 gaattgcaga gattgccgcg agctgtgcct gcaggacaat ctgatagacg tcaaacagcg
    29701 ggtgagattc ggcgctcacc ccttcgccat tcaccagcaa ggtgcgcgtc atatcagcag
    29761 tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc aacaatcttg
    29821 tcgctggctt tgccgtgcgg cagcgccccg cgccagccgc tggcgacaat ggtgtcaaaa
    29881 gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc tatctcgcgc
    29941 tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc ggcaatccca
    30001 caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag cacatccggc
    30061 gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt ttcccaactc
    30121 acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat ttgattgacg
    30181 atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct ggtagccttg cgcacgggct
    30241 tccacctcaa cgaaatagcg cgaatctacc agaatgtgcg cactttcacg gctaataacc
    30301 acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga
    30361 agcaccgcat ccagttgttg caccttaagc cagttgcgca gcgaagcgag taatgtcatc
    30421 tggattttcc ttcttacagg ctatcgatta acaatttgcc tttgcggaac atcatcagac
    30481 gcaggaacac caccatcagc gcagtaatga ttgcccccag cgcgatcccc gccatataaa
    30541 cgcccaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac cattgcactg
    30601 cgcccagcca gacagctgcg gttgagccga caatcgcgcc gaccatatat gacggaatag
    30661 cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc ataaaggcca
    30721 ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta
    30781 aacgacggtc aataatggtc gccagaccca gaccaatcgg cggaataacg atagcgatag
    30841 aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg
    30901 ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca
    30961 tcagcgcgcc cttctcacct gcggcagtca gcacagtacg gataccgccg ttgatccagc
    31021 caccaaaggg agtgatgacg tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg
    31081 gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca
    31141 tccatttcac aaggtaaccg gcaacgatag aaataatcag cgcaccaatg aaggtggacg
    31201 gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc agttgcgtcg
    31261 gctgggtaga catcaacccg ccgataaaac cagccgggaa cgccagtttg ccaccgatag
    31321 agttcgccac aaatgcagca aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg
    31381 actgcgacag ccaggcaaat ttaagtaagg agaggtcgaa accggagaat ttcccgctat
    31441 taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa gcaatcagct
    31501 ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg
    31561 acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc ggattcttaa
    31621 ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt ttaatggcca
    31681 taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt
    31741 tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg
    31801 attcgaaacg ttcgttatct tccggggtaa ctgccacgga atggatgatg atggtcgctt
    31861 cggcgatatc ctgcgccgtc aggcgattct ggatgccgtc cgcgccctgg gtttcaattt
    31921 tcacttcata accggcttct accgccgctt cttccagcac ctgagcggcc ataaaagtgt
    31981 gagctaggcc cattgggcag gcacataagg caatcagttt cttactcatc gttaattcct
    32041 cgttcagtga tagttgacga gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga
    32101 caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc
    32161 ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaaagat tatcgggaga
    32221 gttacctccc gatataacag gaaggattta cagaatgtga cctaaggtct ggcgtaaatg
    32281 tgcaccggaa ccgagaagac ccggatagtc atggacgatg agatacaccg gaatatcatg
    32341 gacatattct ttaaagcgcc ctttatcttc aaatgcggca cggaaaccgg aggctttgaa
    32401 gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa atgtcccgag
    32461 attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg acaatgcgcg
    32521 gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt
    32581 ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca gcccagggcc
    32641 agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt cgaggataat
    32701 ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac
    32761 ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cccgttccgg caccgtaaac
    32821 cgcaataggc ttaccttcga ccggttctgc gccaccaaac tgaatcagat gctctttttt
    32881 cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca gatggctaaa
    32941 accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg tcattgccac
    33001 ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct cgaccttatg
    33061 ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt
    33121 cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc
    33181 gcccacatca ccgactaatg catactttgt cattcttcaa ctgctccgct aaagtcaaaa
    33241 taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct
    33301 ccctgtaaat atcgatctgg gtcacacaat tactttatcg tttcagcacc aattgcagcg
    33361 atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc
    33421 tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg
    33481 cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag aatttgctct
    33541 ggcaacgact gccgggaact ctggggatag cccaggattc acccctctgg atcatcggtg
    33601 tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag ggtcatgccg
    33661 gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccggt tccgccctct gcgctacctg
    33721 gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc
    33781 cgatcatgac cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg
    33841 tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggta
    33901 cacctgttgc ggcggcgttg atattttcgc aaaccttaaa tggcagtagt gaagttccgc
    33961 tatgggatcg tctttttgcg ccgttaatgg cggcagcagc tggtgcactt actaccggat
    34021 tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg gaaatgaccg
    34081 atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat
    34141 ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg ctcgtgctgg
    34201 gtattggcgg atttattctc ggaattctgg gggttattgg tggaccagtt tcgctgttta
    34261 aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact
    34321 ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg
    34381 gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc
    34441 acgttcccgc cgttccagcg gcaataaccg tttcctgcgc tattctcggc atcgtgctgg
    34501 tggtaacacg cgatggctgg ttaagtcttt ttatggcggc agtcgttgta cccaatacca
    34561 cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta gcaggtaagc
    34621 cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataatgtt tagcaaaaaa
    34681 tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct ccccgtaata
    34741 ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac
    34801 tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaatgca ggtgaaatca
    34861 ccctactgcc atcgatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg
    34921 acggtggtca ctggcgagat cgtgattact ggcatcgcaa ttatgaatgg cgcaaaaacc
    34981 gctggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa gcatatgagc
    35041 gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg
    35101 ggcaccgcca ttaaacgcgt cctccaatgg agcacaatgc ctgatgcgac gctagcgcgt
    35161 cttatcaggc ctacaaaccg gctctttaac gcgtagggcg gataaggcgt ttacgccgca
    35221 tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc gtccccacca
    35281 acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc tgttttacgc
    35341 gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc agcagtggaa
    35401 ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg
    35461 gatctaatcc catcagaatg acaataaatg acggcaacat ggtgactgta cgacgcaccc
    35521 acagcgggat atggaagcga atgaatccct gcatcaccac ctgccccgcc agtgtcccca
    35581 ccaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt gccgcagcat
    35641 ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca ccagtatgac
    35701 cagaaaagtg gaacgccgcc gcagctgtag ccatcatcac cagattgaca aaaccggcaa
    35761 tcgtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc
    35821 catgtaaatg ctgagtgagc gaagagtgca aataaatcac atgcggcata atcgtcgccc
    35881 ctaacacgcc tgctgccagg aagaccgcct ccgaagtagg taaactcggg atcaccattc
    35941 ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg
    36001 caacaaacaa cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca
    36061 gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac
    36121 caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg
    36181 caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag tgatcacgaa
    36241 tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg atctgaatca
    36301 gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg aagctagcac
    36361 ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg
    36421 caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc
    36481 gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctatgctaa
    36541 acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctctga tagaccaaga
    36601 ttatgattac gaaaagattg atgacttgaa tgttatggtt atgtttaatg ttagcacatt
    36661 tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat cactattttt
    36721 gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt
    36781 acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc
    36841 cttcattttg tactggcact tgccgttgtt gtgattctcg cactgctggt aagcagcgac
    36901 cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg
    36961 tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc cgaaatgttc
    37021 gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag catgaatgat
    37081 caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg
    37141 atcggtattc tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg
    37201 ctctccaaag tcaacggtat gggcaaactg gaatccttta acgccgtcag ctccctgatt
    37261 ctgggtcagt cagaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgtaat
    37321 cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat cgttggtgca
    37381 tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtactgaa catgttcagc
    37441 acctttatcg tgctgtcgct gatcaatcct taccgtgttg atgccagtga agaaaacatc
    37501 cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg
    37561 gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc
    37621 gccgcgctga acgcactgtt tgccaccgtt actggctggt ttggctacag catctccttc
    37681 cagggcatcc tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt
    37741 gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg
    37801 atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg catcatctct
    37861 gtgttcctag tttccttcgc taacttctct tcaatcggga ttatcgcagg tgcagttaaa
    37921 ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc
    37981 tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta aaaccataca
    38041 taaaaaagcc ggggatcatt ccccggcttt tttacgcctg ttaatcagct aatggctgcg
    38101 ggcgaccaat caaataccct tgcagatatt gcaccccgag cttatgcaat agcgcctgct
    38161 gctgttgcgt ctcgacaaac tccgcgacca cactcaatga cttcgctttc gccagatcgg
    38221 taattgatct cacaatcatc gcatccagcg tattcgtgac aatatctttc acaaagacgc
    38281 catcaatttt gatgatatca gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg
    38341 tgccaaagtc atcaatcgca atccggaaac caaacttatg cagctgctcg atgttgtaca
    38401 tgctgctttc tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg accgcctgcg
    38461 gggagatgtg ataacgctta aacagacgaa taatccgccc ggcaatattc ttttgcagca
    38521 gcgtgagcgg cattaaattg actgaaaagc gcggtccttt tttgtcgcaa gggtgtgttg
    38581 ccagccactt caacaaggat tccagcactt gcaaatcaaa acgcgcgcta aggttaaact
    38641 gagcaataag gggcagaaac ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg
    38701 cgaggatctc atcataacct tcaccctctt tgttgcgaat tggctgggcg tagagcagca
    38761 aatcaccctg atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt ttagtggtct
    38821 gcccggaaac catctcctct ctgctatcca gcgccagcac atgatgatgt gcgcaggatt
    38881 gctccgccag ccagcttaac tgccccaaca agggttgcag ggtttcctga tttccatcaa
    38941 aacgccccca ggcagcacca tagcccatat ccagcccggt attgttccag tgaatttgcc
    39001 gactattcag gatgttaacc atatgctgga gtcgcccttc cgtttccggc ccgctcagca
    39061 ctaacagcag ttcactaccc ggcaattgat acaacttttc gttttcctgc atcaacggca
    39121 gcagcgtacg gcaaattgag cggatacagt gaacgcgcat cattaagccg taatgacgac
    39181 tcataaactc aagattatca atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg
    39241 gcgcttgttc caacgcacga aagttgggta ggagcgttaa cggatctgtt agcgcctgca
    39301 aatgccagcg gcgattaagc cattcactgc gatgataaat gcgcaccata tagagcaggc
    39361 aaacgctaaa ggaaatcagc accgcgagaa taaatgccag cgaatattcg gtttccaccc
    39421 cttgcaaaaa gttctggttg taattcagaa ggcaaagcgt tgaaacagcc caggtgagat
    39481 ttaaaaacgg atagcgaaga ttaccgaccc cgagggtaaa gatgatgaag aaaacgggta
    39541 ccaggtaacc ggcaataaag tcgttttcat aaggtgtgca caacagaagt agcagcacgc
    39601 ttaacgctgc cagccagctt aaggtaaatg cgagtgagat agtagaaaag catgttgtaa
    39661 atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg
    39721 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    39781 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    39841 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    39901 aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag
    39961 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    40021 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    40081 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    40141 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    40201 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    40261 caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct
    40321 aacccggacg tagactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt
    40381 tgcagcgagg gttggaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
    40441 gccatgcaag cgctctccca gctgagctat agccccacga tgcgtttacg taccaaattt
    40501 gctgggtgca aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
    40561 gcaagcgctc tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc
    40621 ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc
    40681 gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat
    40741 gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc
    40801 agggaattgt tatgttcaag gagaggatga caccagatga acttgccaga ctgaccggct
    40861 atagccgcca gaccattaat aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac
    40921 ctggcgtcca gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata
    40981 ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc
    41041 cgcttgaagt attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt
    41101 ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg
    41161 atagcaaata atatgaaaag attacgcaat aaaatgacca ccgaagaact ggctgaatgc
    41221 ctcggtgtgg ctaaacaaac tgttaaccgt tggaccagag aaaaaggctg gaaaacggaa
    41281 aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc
    41341 gagtttattc agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag
    41401 agaattgctg aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg
    41461 atagataata tgacagacat tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga
    41521 attcgcaatt tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg
    41581 atattatctc ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc
    41641 aataaaatcc agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac
    41701 ggtaacatcc agtgctggag actgccccgc accggttacg gcgacacgca gcggcatacc
    41761 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
    41821 ttcagcggtc cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg
    41881 cgctaccgga cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta
    41941 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
    42001 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
    42061 ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
    42121 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta
    42181 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
    42241 cagatagttc agcagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
    42301 tgccccgtga cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc
    42361 gtaaaccggc accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt
    42421 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac
    42481 acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg
    42541 gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
    42601 gttagcaaaa cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca
    42661 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
    42721 gcgctcttta gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac
    42781 cgcgttgtag cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct
    42841 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
    42901 ggtgtcttca atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta
    42961 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
    43021 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
    43081 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc
    43141 taccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
    43201 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
    43261 ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc
    43321 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag
    43381 tctccgccgt gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc
    43441 ccttacaagg agggggtcgg cggttcgata ccgtcatcac ccaccacttc gggtcgttag
    43501 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcg
//
LOCUS       DAECNO010000026        41265 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.026, whole genome shotgun sequence.
ACCESSION   DAECNO010000026 DAECNO010000000
VERSION     DAECNO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.026"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1545
                     /locus_tag="KKO08_001415"
     CDS             <1..1545
                     /locus_tag="KKO08_001415"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD6317719.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
     gene            1704..2263
                     /locus_tag="KKO08_001416"
                     /pseudo
     CDS             1704..2263
                     /locus_tag="KKO08_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            2264..3913
                     /locus_tag="KKO08_001417"
     CDS             2264..3913
                     /locus_tag="KKO08_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBD6317720.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3918..4694
                     /locus_tag="KKO08_001418"
     CDS             3918..4694
                     /locus_tag="KKO08_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBD6317721.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4969..7818
                     /gene="rcsC"
                     /locus_tag="KKO08_001419"
     CDS             4969..7818
                     /gene="rcsC"
                     /locus_tag="KKO08_001419"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBD6317722.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8018..8668)
                     /gene="rcsB"
                     /locus_tag="KKO08_001420"
     CDS             complement(8018..8668)
                     /gene="rcsB"
                     /locus_tag="KKO08_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBD6317723.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8685..11357)
                     /gene="rcsD"
                     /locus_tag="KKO08_001421"
     CDS             complement(8685..11357)
                     /gene="rcsD"
                     /locus_tag="KKO08_001421"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBD6317724.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12095..13216
                     /gene="ompC"
                     /locus_tag="KKO08_001422"
     CDS             12095..13216
                     /gene="ompC"
                     /locus_tag="KKO08_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBD6317725.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13328..14383
                     /gene="apbE"
                     /locus_tag="KKO08_001423"
     CDS             13328..14383
                     /gene="apbE"
                     /locus_tag="KKO08_001423"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBD6317726.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14457..15521
                     /gene="ada"
                     /locus_tag="KKO08_001424"
     CDS             14457..15521
                     /gene="ada"
                     /locus_tag="KKO08_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBD6317727.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15521..16171
                     /gene="alkB"
                     /locus_tag="KKO08_001425"
     CDS             15521..16171
                     /gene="alkB"
                     /locus_tag="KKO08_001425"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBD6317728.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16247..17890
                     /gene="yojI"
                     /locus_tag="KKO08_001426"
     CDS             16247..17890
                     /gene="yojI"
                     /locus_tag="KKO08_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBD6317729.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18108..18467
                     /locus_tag="KKO08_001427"
                     /pseudo
     CDS             18108..18467
                     /locus_tag="KKO08_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            18701..19720
                     /locus_tag="KKO08_001428"
     CDS             18701..19720
                     /locus_tag="KKO08_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBD6317730.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQPVQKEV
                     ADIAL"
     gene            complement(19870..20358)
                     /gene="eco"
                     /locus_tag="KKO08_001429"
     CDS             complement(19870..20358)
                     /gene="eco"
                     /locus_tag="KKO08_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBD6317731.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            20766..21260
                     /gene="napF"
                     /locus_tag="KKO08_001430"
     CDS             20766..21260
                     /gene="napF"
                     /locus_tag="KKO08_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBD6317732.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21250..21513
                     /gene="napD"
                     /locus_tag="KKO08_001431"
     CDS             21250..21513
                     /gene="napD"
                     /locus_tag="KKO08_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBD6317733.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            21510..23996
                     /gene="napA"
                     /locus_tag="KKO08_001432"
     CDS             21510..23996
                     /gene="napA"
                     /locus_tag="KKO08_001432"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBD6317734.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELVEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            24003..24698
                     /gene="napG"
                     /locus_tag="KKO08_001433"
     CDS             24003..24698
                     /gene="napG"
                     /locus_tag="KKO08_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBD6317735.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            24685..25548
                     /gene="napH"
                     /locus_tag="KKO08_001434"
     CDS             24685..25548
                     /gene="napH"
                     /locus_tag="KKO08_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBD6317736.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            25545..25994
                     /gene="napB"
                     /locus_tag="KKO08_001435"
     CDS             25545..25994
                     /gene="napB"
                     /locus_tag="KKO08_001435"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBD6317737.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            26004..26606
                     /gene="napC"
                     /locus_tag="KKO08_001436"
     CDS             26004..26606
                     /gene="napC"
                     /locus_tag="KKO08_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBD6317738.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            26625..27242
                     /gene="ccmA"
                     /locus_tag="KKO08_001437"
     CDS             26625..27242
                     /gene="ccmA"
                     /locus_tag="KKO08_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBD6317739.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            27239..27901
                     /gene="ccmB"
                     /locus_tag="KKO08_001438"
     CDS             27239..27901
                     /gene="ccmB"
                     /locus_tag="KKO08_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBD6317740.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            27943..28680
                     /gene="ccmC"
                     /locus_tag="KKO08_001439"
     CDS             27943..28680
                     /gene="ccmC"
                     /locus_tag="KKO08_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBD6317741.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            28677..28886
                     /gene="ccmD"
                     /locus_tag="KKO08_001440"
     CDS             28677..28886
                     /gene="ccmD"
                     /locus_tag="KKO08_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBD6317742.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            28883..29362
                     /gene="ccmE"
                     /locus_tag="KKO08_001441"
     CDS             28883..29362
                     /gene="ccmE"
                     /locus_tag="KKO08_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBD6317743.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            29359..31302
                     /gene="ccmF"
                     /locus_tag="KKO08_001442"
     CDS             29359..31302
                     /gene="ccmF"
                     /locus_tag="KKO08_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBD6317744.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            31299..31856
                     /gene="dsbE"
                     /locus_tag="KKO08_001443"
     CDS             31299..31856
                     /gene="dsbE"
                     /locus_tag="KKO08_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBD6317745.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            31853..32905
                     /gene="ccmH"
                     /locus_tag="KKO08_001444"
     CDS             31853..32905
                     /gene="ccmH"
                     /locus_tag="KKO08_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBD6317746.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(33116..33763)
                     /gene="narP"
                     /locus_tag="KKO08_001445"
     CDS             complement(33116..33763)
                     /gene="narP"
                     /locus_tag="KKO08_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBD6317747.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            34083..36674
                     /gene="yejO"
                     /locus_tag="KKO08_001446"
     CDS             34083..36674
                     /gene="yejO"
                     /locus_tag="KKO08_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBD6317748.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            complement(38076..38354)
                     /locus_tag="KKO08_001447"
     CDS             complement(38076..38354)
                     /locus_tag="KKO08_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBD6317749.1"
                     /translation="MIHDSKAEDLESKGLYRRAAARWAEVMQQVNTDKEREQAVKRRA
                     ECIHKAARSPVILDNFGGLREAANRTQADMGIEIAKSVWRNYQTDKVL"
     gene            complement(38357..38872)
                     /locus_tag="KKO08_001448"
     CDS             complement(38357..38872)
                     /locus_tag="KKO08_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HBD6317750.1"
                     /translation="MIRGDSIYGIPKKKTQTTQQDKTTSAHKVIPGNQNAPQSRQQKP
                     TGATPWRHMTKRQGKNRRRANRLIELWPELFSLESPKPLKVGIFEDLIQDIAVRAQAF
                     GSGALRAALASYAHSPRYYRALMAGGVRYDLNSQPCGEVTQQEQQNAEARLMALKEKR
                     KRKSNKEQAGV"
     gene            39010..40223
                     /locus_tag="KKO08_001449"
     CDS             join(39010..39301,39301..40223)
                     /locus_tag="KKO08_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6317751.1"
                     /translation="MTKNMRFSPEVRQRAVRMVIESQNDYDSQWAAISSIAPKIGCTP
                     ETLRTWLRQYERDAGAGDGGLSTTERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPVEKIAPLVDTLRGTHGVGPVCHELAVAPSTYYRHRAHQRHPDKRSQRAQRDDLLK
                     PEIQRVYDENHSVYGVRKVWRQLLREEVTVARCTVERLMKVMGLAGVRRGKKVRTTVS
                     RKEAAAGDRVNRQFVAECPNQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSS
                     MKTTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHIPPAEAEKA
                     YYASIGNGDLAA"
     misc_feature    39291..39407
                     /locus_tag="KKO08_001449"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(40419..41234)
                     /locus_tag="KKO08_001450"
     CDS             complement(40419..41234)
                     /locus_tag="KKO08_001450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317752.1"
                     /translation="MSRTYISLYDLISGEIIKSGPLKGMALGIGLPITFAFIWNFATD
                     HNKKKQDYLLVLIILLIICGGINFGFLGGVFTHLSKIQFTWRFIPFLLFSILLILHAS
                     GRIKLKYILLIFTSTCLMTSAITVPKKSSHNLTIDIAASANYKDYVLSNAPELGDRIK
                     ELKCDNGLSYAFSRTLGSNGLPVFTFSIPTKTTCSIPFMSYKSLELDGAKKYSRDGYF
                     KAELTKGNYIIRIDTTYRFKQVVLISFIVSICTILTILLFALKRKWKWKWKWK"
BASE COUNT         9707 a         9996 c        11364 g        10198 t
ORIGIN      
        1 ttaacctcgt cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat
       61 gaaatgatct acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg
      121 ctggggcgta acaatgtgcg taccagctcc agtctgtcgt ttatcagcta tgaccaggcg
      181 ctctctagcg agccggttgc gcctggcgcg actaaccgca gcgagcggcg agtaaaaatg
      241 cttgaacgtc cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc
      301 gataaacaag gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt
      361 atcaccgcgc gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt
      421 tcggaaaaaa atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa
      481 ccggcggcag gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact
      541 aaatttgcag gcgctgagat gcgccagacg ctgacgctgc acaaaggggc gaattatatt
      601 tcgctgacgc agaatattca gcaatctggc ttgttaagtg cagaactgca acaaaatggg
      661 caagtgcagg acagcattag cacaaaactg tcttttgtgg acaacagctg gcccgttgaa
      721 cagcagaaaa atgtcatgct cggcggtggc gagaacgcgc tgatgttgcc cgagcaggcg
      781 agtaatatcc ggctacaaag tagtgaaacg ccgcaggaga ttttccgcaa caatcttgat
      841 gcgttagtcg atgaaccgtg gggtggggtg atcaacaccg gtagccgtct gatcccgctc
      901 agtctcgcct ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag
      961 atgattcagg ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc
     1021 tggtggggtg aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgac
     1081 tggcaggcca gccaggcgct cggcgtaacg caacaaccgg aatactggca gcatatgctc
     1141 gacagttatg ccgagcaggc ggataacatg ccgttattgc atcgggcgct ggtgctggcg
     1201 tgggcacagg agatgaatct gccgtgcaaa acgttgttga aaggggggga tgaagctatc
     1261 gcccggcgcg gaactaaaga tgaagatttc tctgaggaag acatccgcga tatcaatgac
     1321 agcctgatcc tcgatacacc ggaatctcca ctggcagatg cggtggcaaa cgtcttaacc
     1381 atgacgttgc tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat
     1441 gcgtgggata aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgctcaat
     1501 agcggtggtg acgctaccca gacggccgct attttaatgg tttgaccgct gagcaatcca
     1561 ctattgagcg cgcgctggcc atgaactggc tggcgaaata tatggcgaca atgcctccag
     1621 ttgttttgcc tgcgcctgcg ggcgcatggg ctaaacataa gttaactgga gggggcgaag
     1681 actggcgttg ggttggttag ggtgtgccgg acattctctc ttttggtgac gaattatcgc
     1741 cgcaaatgtg caggtccgct ggcgtgaacc ggcaaaaacg gctcaacaaa gtaacattcc
     1801 ggtgaccgtt gaacgccagt tgtatcggct tatccccggt gaagaagaga tgagctttac
     1861 tctgcagccg gtgaccagca atgagattga cagcgatgcg ctgtatctcg atgaaattac
     1921 gcttaccagc gagcaggatg cagttctgcg ctatggtcag gtggaagtac cgctcccgcc
     1981 gggagccgac gttgagcgca caacatgggg catttcagtc aataaaccca acgccgcgaa
     2041 acagcagggg caattgctgg aaaaagcgcg taatgaaatg ggcgaactgg cctatatggt
     2101 gccggtgaaa gaactgacgg gaacggtcac tttccgccat ttgctgcgct tctcgcaaaa
     2161 agggcaattc gttctgcctc ctgctcgtta tgtgcgttcc tatgcacctg cgcagcaaag
     2221 tgtcgcggca gggagtgaat ggaccgggat gcaggtgaaa taagtgaact ggcgcagaat
     2281 cgtctggcta ttggcgttgg ttactctgcc aacgcttgcg gaggagacgc cgttacaact
     2341 ggtgctgcgc ggtgcgcagc acgatcaact gtatcagctg tcgtcatcag gcgtgaccaa
     2401 agtctcagca ttacctgact cgctcaccac gccgttaggg agtttgtgga aactgtatgt
     2461 ttacgcctgg ctggaggata cccatcaacc ggagcagccc taccagtgtc gcggaaactc
     2521 cccggaagag gtttattgtt gtcaggctgg ggagagcata acgcgcgata ccgcgctggt
     2581 acgttcctgt gggctctatt ttgcgccgca gcggttgcat attggcgcag atgtgtgggg
     2641 gcaatactgg caacagcgtc aggcacctgc gtggttagcg tctcttacaa cgctgaagcc
     2701 agaaacctcg gtaacggtta aatcgctgct tgattcatta gcgacgttac ctgcgcaaaa
     2761 caaagctcag gaagtcttac tggatgtggt gctggacgag gcgaaaatcg gtgtcgcatc
     2821 catgctcggt agtcgggtac gggtgaaaac ctggagctgg tttgccgatg ataaacagga
     2881 aatccgtcag ggtggttttg ctggctggtt aaccgatggt acaccgctgt gggttactgg
     2941 cagtggcacc agcaaaacgg tactaacccg ttatgcgacg gtgcttaatc gggttttgcc
     3001 agtgccgacg caggttgcca gtgggcaatg tgtagaggtg gagctgtttg cccgctatcc
     3061 gctgaagaaa atcacagcgg aaaagagtac gacggcagta aaacccagcg tacttaatgg
     3121 gcgctatcgt gtgacgttta ctaatggtaa ccacattacg tttgtcagcc acggtgagac
     3181 gaccttactg agcgaaaagg gcaaactgaa attgcagtcg catctcgatc gtgaagagta
     3241 cgttaccaga gttctggatc gcgaggcgaa aagtacgcca cctgaggcag cgaaagccat
     3301 gactgtggcg atacgtactt ttttacagca aaacgccaat cgggaaggtg attgcctgac
     3361 catccccgac agcagcgcca cgcagcgtgt ttctgcttcg ccagcgacca cgggggcgcg
     3421 aacgatgacc gcctggacgc aggagctcat ctatgcaggc gatccggttc attatcatgg
     3481 cagtcgcgct accgaaggga cactttcctg gcggcaagcc acggcccagg cggggcaggg
     3541 ggagcgttac gaccagatac tcgcgtttgc ttatcccgac aacagcctta gccgctgggg
     3601 agctccacgc tcaacctgcc agttattacc caaagcgaaa gcctggctgg cgaaaaaaat
     3661 gccgcagtgg cggcgtatat tacaaggcga gacggggtac aacgaaccag acgtgtttgc
     3721 ggtctgtcgt ttagtctcag gtttccccta taccgatcgg cagcagaaac ggctgtttat
     3781 tcgtaacttc tttacgcttc aggatcggct ggatttaacc catgagtatc tgcacttggc
     3841 ctttgatggt tatcccaccg ggcttgatga gaactatatc gaaacgctga cccgacaatt
     3901 attaatggac taatgctatg cgaaagattt ttcttccgtt gttactggtg gcactttcgc
     3961 ctgtcgctca cagtgaaggt gtgcaggaag tcgagattga cgcaccgctc tctggctggc
     4021 atccggcaga gggcgaagat gccagttttt cacaatcgat caattacccg gcgtcgtccg
     4081 tcaacatggc agatgatcaa aatatctcag cgcagatccg cggcaaaata aagaattatg
     4141 ctgcggcggg taaagttcag cagggccggc tggtggtcaa cggtgccagt atgccgcaga
     4201 gaattgaatc cgatggttca tttgcacgcc cttatatttt cactgaaggc agcaacagcg
     4261 tgcaggtcat cagcccggat gggcaaagcc ggcaaaaaat gcagttttac tcaacgccgg
     4321 gtacaggaac tattcgtgca cgtttacggc tggttctctc gtgggatacg gacaataccg
     4381 atctcgatct ccatgttgtc acgcccgatg gcgaacacgc ctggtacggt aacaccgtgc
     4441 tgaaaaacag tggtgcactg gatatggatg tcacgacggg gtacggaccc gagattttcg
     4501 ccatgccagc accagttcac gggcgctatc aggtgtatat caactactat ggtgggcgca
     4561 gcgaaacgga attgacgacc gcccaactga cgctgatcac cgatgaagga tcggtcaatg
     4621 agaaacagga aacatttatt gtaccgatgc gtaatgctgg cgagctgacg ctggtgaaaa
     4681 gttttgactg gtgatccatc ccttcacaca acctggctct ctcttttttc agataagact
     4741 gctgggcgat ctgccgacgc caggatataa ccattaacaa gcgtcggcag attggtcatt
     4801 tcttattttt cgtactgaga actgtccatc tactccggct ttcctggtta aggcaatggt
     4861 ttctaagcga tacgcattat tgcaatggtg acagaacatt gcggtaacac gcttttaccg
     4921 ctaccttaac cacactccat cggtcacctg aggcggagct tctccccttt gaaatacctt
     4981 gcttcttttc gtacaaccct gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg
     5041 ctctggctgt tgattgcttt ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga
     5101 gaatcggaaa ttcgtcagga atttaatctg agttccgatc aggctcagcg ctttattcaa
     5161 cgcacctctg atgtgatgaa agagcttaag tacatcgccg aaaatcgctt atcggcagaa
     5221 aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg
     5281 ctgtttgccg actccgattg ttccgcaatg agtaacacct ggcgaggttc tctggagtca
     5341 ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg cggcttacga tctcaaccgg
     5401 gtatttttaa tcggcagtga taacctctgc atggccaatt tcggtctgcg tgatatgcca
     5461 gtggaacgcg ataccgcgtt gaaagcgttg catgaacgca tcaataaata tcgaaatgca
     5521 ccacaagatg atagcggcag taacctctac tggatcagcg aaggtccgcg cccttgcgtc
     5581 gggtattttt acgcgttgac gccagtttat ctggcgaacc ggttgcaggc gcttttgggt
     5641 gtcgagcaga ccatccggat ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt
     5701 accattcttg atgaaaatgg tcataccctg atttcgctta ccggaccaga aagtaaaatt
     5761 aagggcgatc ctcgctggat gcaggaacgc tcctggtttg gctatacgga agggttccgg
     5821 gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg
     5881 gttgataagg tgctggaacg cattcgcatg ttgatcctta acgcaatttt gctgaatgtg
     5941 cttgccggag ctgcattgtt tactctcgcg cggatgtacg agcgacgtat tttcattccg
     6001 gcggaaagcg acgccctgcg actggaagaa catgagcagt tcaatcgcaa gattgtcgcc
     6061 tccgcgccag tgggtatctg cattttgcgt accgctgatg gcgtcaatat tttaagtaac
     6121 gaactggcgc atacctatct caatatgctt acgcatgagg accgccaacg actgacgcaa
     6181 attatctgtg ggcagcaggt caattttgtt gatgtcctga ccagcaacaa taccaatctg
     6241 caaatcagct tcgtccattc gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg
     6301 gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg agatggcaca agcagcggaa
     6361 caggcgagcc agtcaaaatc gatgttcctt gccaccgtca gtcatgagct gcgaacgccg
     6421 ctgtatggca ttatcggtaa cctggatctg ttgcaaacca aagagttacc gaaaggcgtc
     6481 gatcgtctgg tgacggtaat gaacaactct tccagcctgt tgttgaaaat tatcagcgat
     6541 attctcgatt tctcgaagat tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca
     6601 ccgcgtgaag tgatgaacca catcaccgcc aactatttac cgctggtggt acgcaagcag
     6661 ttaggcttgt actgctttat tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg
     6721 cgtttacagc aggtcatctc caacctgttg agtaacgcca taaaattcac cgataccggc
     6781 tgtatagttt tgcatgttcg cgctgatggc gattatctct ctatccgtgt tcgcgatacc
     6841 ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg atcccttctt ccaggttgga
     6901 acgggcgtac agcgaaactt ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg
     6961 atcagcatga tggacggcga tatctcggta gattcagaac cgggaatggg cagccagttt
     7021 accgtgcgta ttccgttgta cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg
     7081 agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc
     7141 agtttgcagc gcagcggcat cgtcgttaca acatacgaag ggcaggaacc gactcccgaa
     7201 gatgtgttaa tcactgacga ggtagtgagt aaaaaatggc agggcagagc ggtagtgacc
     7261 ttctgtcgtc gccatattgg tattccgctg gagaaagcgc caggggagtg ggtacacagt
     7321 gtggctgctc cgcatgagct accggcattg ttggcgcgta tttatttgat cgagatggag
     7381 agcgacgatc ctgctaacgc tctgccgtcg acggacaaag cggtcagcga taacgacgat
     7441 atgatgattc tggtcgtgga tgatcatccg attaaccgac gtctgctggc agatcagttg
     7501 ggatcgttgg gctatcaatg taaaaccgcg aatgatggcg tcgatgcgct taatgtactt
     7561 agtaagaatc atattgatat cgtgcttagc gacgtcaaca tgccaaatat ggatggttac
     7621 cgcttgacgc aacgcattcg tcagttggga ctgacgttgc cggtaatcgg agtaactgct
     7681 aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg gtatggacag ctgcctctcg
     7741 aagccggtaa cgctggatgt gataaaacag acgctgacgg tatatgccga gagggtcagg
     7801 aaatcgcggg attcgtagga cggataaggc gtttacgccg catccggcat tcagtgcaaa
     7861 tgccagataa gacactgacg cgtcttatct ggcctacttt aatgtaatct gttgaaataa
     7921 tgggaatcgt aggccggata aggcgtttac gccgcatccg gcaatcggtg caaatgccag
     7981 ataagacact aacgcgtctt atctggccta cagatgatta gtctttatct gccggactta
     8041 aggtcacaga agagagataa ttcagcaggg cgatatcgtt ctcgacgccc agcttcatca
     8101 tcgcagattt cttctggcta ctgatggttt taatactgcg gttcagcttt ttagcgatct
     8161 cggtcactag gaagccttcc gcaaacaggc gcagaacttc actctctttt ggcgagagac
     8221 gcttgtcacc gtaaccacca gcactgattt tttccaacag gcgagaaacg ctttccgggg
     8281 taaatttctt ccctttctgc agcgcggcga gagctttcgg cagatcggtc ggtgcacctt
     8341 gtttcagcac gatcccttcg atatccagat ccaataccgc actaagaatc gccgggttgt
     8401 tgttcatagt cagaacaatg atcgacaggc ttgggaaatg gcgcttgatg tacttgatta
     8461 aggtaatgcc atcgccgtac ttatcgccag gcatggagag atcggtaatc aacacatgcg
     8521 catccagttt cggcaggttg ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat
     8581 tcacccactc aatttgctca agtgatttgc gaataccgaa caagactatc ggatggtcat
     8641 cggcaataat tacgttcata ttgttcatgt aataggctac cttgctacag caagctcttg
     8701 acataactgt caatgtcgct gatgtatttt tctattcctg gaacatcctt ctcacgaatc
     8761 agatgttcca gcgtttcaca taactgcttg ccgggtacca gatttagcat ggcaaatacg
     8821 cctttaagac gatgagccgt ttgggctaac gcagcaaagt cactggttgc tgcttcagta
     8881 tacagcctct taacatcatc cggtactgtg tctacaaaga gcgcataata gccgctggca
     8941 tggagttgcg cattttcatc tccgcccaga ggcgattctg tcacctcttc ctgtgccagt
     9001 tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg
     9061 cacaattgac caggcccaat ttctcgtacg ccagactcat catcgcttaa aagcaagcca
     9121 gaggcagtaa gattagacgg attatccgtt aaaaagatat cataatcttg actaattaat
     9181 ctttcatcgg gtgtgataca ggttgcaccc caattttcta acaggcgagt gacaatattc
     9241 cgaatttctg ccgaagtaac atccaccatt acgcagacat catccagtaa acgctcttct
     9301 tcctcttcaa cttccgggtc agcggcgagc attttgatat gcacagagta gcgtgtacca
     9361 agcccatccc gcgttttgat gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc
     9421 agccagaatg ccagcgggtc cgcctttcca taacgatcgt tttgggtctg gttgataaac
     9481 gggaagtgca aattatccat ttcatgaata cttacgcctt ctcccgtgtc cagaatgcgg
     9541 aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct caagggtgat ttttcccaat
     9601 tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga
     9661 tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa tcagcagttg cagaccttta
     9721 cgcttgatgg caggcaacac tgaaggcaca acttcatcaa ttaaatcctg cacggagaac
     9781 agcaccgtct cacttttcca gctatcatcc gcaagcatgt tcgctaactg tatttcttcg
     9841 accagccgca ccagcacatc tgcctgattc gccagttgtt tgctttccgg ggcgttgagt
     9901 ttagccgcac tctccgccag ggactgtgcg ggttctttca gcgcatcgcc aatgtttttc
     9961 ataaaggtca tccgcccctg ctggtttttc tcatacagac gctgcgcctg cttgagtttc
    10021 ttgtttacca gcacttcgcg atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg
    10081 acctggctgc ggaacatgcg gatctcatac agctcgttat tgatcgtcgc ctgaataatc
    10141 ccctgatgct gttccgccat ggtggtgatg ttttgcagat tcagatgcgg cagcaaatga
    10201 tcggcaattt tgttacttat gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg
    10261 agcggcaaca gtgagactat ctcttcattg attgcccgta aaatgcgcaa ttcgttattg
    10321 accgccgtgc tggggacgct ttctgtactg cggctggaga aatggcggaa tgtggtatag
    10381 ccaaataacg ccagcgccag caaaccgatg ttcagcagca gtggcagcag aatgttttgc
    10441 aacgtatcca gcaataaggt gccataagga acctgccaga ccaggcgcat atcggtagag
    10501 ttgagtgccg aggagatttc aatcttcgta ctgttaaagt ggatactgac gctatccgtc
    10561 ccttctttct cattatcatt gtttcccgtt gccgtcgcgt ctggctcaag gcggaaactg
    10621 tccagcggca tacccggtgg gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt
    10681 gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa agtaatgacc gttctgccag
    10741 gctaggcggc gcatgttaga aaaactttcg cgttcatcga gggcgttggc ctgttgtaac
    10801 atttctgcac gacgtgaatc aacaatgtca ctgacggtcg attctttaaa tccggaggtg
    10861 agatctttaa ggggtagggt tgagatcagc accagactgt tatcctgacc attcaggtaa
    10921 tacatcgacc acggtacatt ttctgcaccc cacaatgtat ccagataagt ggacatccgc
    10981 tgagtcatct caagcgttga gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg
    11041 cgtggtttct ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg
    11101 gaggaggggg aggtcgtcgc ggcaatgttg tcgtagatct gcctggtcac gtaacgccag
    11161 gtatcgacgc gcttttggat tgcgtgggta atgtcgacaa tctggtaact tttatctttc
    11221 agccaagcgt taacggcact ttgtaccatt acccccatcg tcaccagtaa cacaatgatc
    11281 aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt
    11341 gtctctttct gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag
    11401 tataaagggt acagtgtgaa attccagact cttacctgat tgatgattct ctattctccc
    11461 gtgatattta tcatcctgta tggggggaaa tagatgtaca agcgccattt tgctgtacaa
    11521 ataacctaca aaaagcccaa cgaaattaat tgcaaataaa caaagattgc tggaaattat
    11581 gctgatgtta attatttgtg aaatagttaa caagcgttat agtttttctg tggtagcaca
    11641 gaataatgaa aagtgtgtaa agaagggtaa aaaaaccgaa tgcgaggcat ccggttgaaa
    11701 taggggtaaa cagacattca gaaatgaatg acggtaataa ataaagttaa tgatgatagc
    11761 gggagttatt ctagttgcga gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt
    11821 aagaataagt tattgatttt aaccttgaat tattattgct tgatgttagg tgcttatttc
    11881 gccattccgc aataatctta aaaagttccc ttgtatttac attttgaaac atctatagcg
    11941 ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt ctgcattttt
    12001 ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt ggcataaaaa
    12061 agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac tgtccctcct
    12121 ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca acaaagacgg
    12181 caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg acgacaagtc
    12241 tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc aggttactga
    12301 ccagctgacc ggttacggcc agtgggaata tcagatccag ggcaacagcg ctgaaaacga
    12361 aaacaactcc tggacccgtg tggcattcgc aggtctgaaa ttccaggatg taggttcttt
    12421 cgactacggt cgtaactacg gcgtagttta cgacgtaact tcctggaccg acgtactgcc
    12481 agaattcggt ggtgacactt acggttctga caacttcatg cagcagcgtg gtaacggctt
    12541 cgcaacctac cgtagcaccg acttctttgg tctggttgac ggtctgaact tcgctgttca
    12601 gtatcagggc aaaaacggta gcccggaagg tgaaggcatg accaacaacg gtcgtgaagc
    12661 tctgcgtcag aacggtgacg gcgttggcgg ttctattact tatgattacg aaggcttcgg
    12721 tatcggtgct gcagtttcca gctccaaacg tactgatgac cagaacttcg gtctgaaccg
    12781 ttatgatgag cgttacatcg gtaatggcga ccgcgctgaa acctacaccg gtggtctgaa
    12841 atacgacgcg aacaacatct acctagctgc tcagtacacc cagacctaca acgcaactcg
    12901 cgtaggcaac ttgggttggg cgaacaaagc acagaacttc gaagctgttg ctcagtacca
    12961 gttcgacttc ggtctgcgtc cgtccctggc ttacctgcag tctaaaggta aaaacctggg
    13021 tgtcatcaat ggtcgtaact acgacgacga agatatcctg aaatatgttg atgttggtgc
    13081 gacctactac ttcaacaaaa acatgtccac ctacgttgac tacaaaatca acctgctgga
    13141 cgacaaccag ttcactcgtg acgctggcat caacactgat aacatcgtag ctctgggtct
    13201 ggtttaccag ttctaatctc gatggatatc gaacaaaggg cctgcgggcc cttttttcat
    13261 tgttttcagc gtacaaacac agttttttgg tgtactcttg cgaccgttcg catgaggata
    13321 atcacgtatg gaaataagct ttacccgcgt ggcactgctg gttgccgcgc tcttctttgt
    13381 tggttgcgat caaaaaccac aacccgccaa aacccacgct actgaagtta ccgttcttga
    13441 aggcaaaacc atgggtacct tctggcgtgc cagcatcccg ggcattgatg ccaaacgcag
    13501 tgccgaactt aaagaaaaga ttcagaccca gctggacgcc gacgatcagc tgctttcgac
    13561 ctataaaaaa gattccgcgc tgatgcgctt taacgactcg caaagtttgt cgccgtggcc
    13621 ggtaagtgaa gcgatggccg atatcgtcac cacctcgctg cgcattgggg caaggaccga
    13681 tggtgcgatg gatattaccg tcgggccgct ggtgaatctg tggggctttg gcccggaaca
    13741 acagccggtt caaattccga gccaggaaca gatcgatgcg atgaaagcca aaaccggctt
    13801 acagcacctg acggtcatca atcagtcgca tcagcaatat ctgcaaaaag acctgccgga
    13861 tttatatgtc gatctctcca ccgtcggtga aggttatgcg gcggatcacc tggcacgctt
    13921 gatggagcag gaagggattt cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg
    13981 tggtatgaac ggtgaaggcc agccgtggcg ggtagcgatt caaaaaccaa ccgataaaga
    14041 aaacgcggtt caggctgtgg tggatatcaa cggtcatggg attagcacct ctggcagcta
    14101 ccgtaactat tacgaactgg acggcaaacg tctttcccat gttatcgatc cgcaaaccgg
    14161 gcgtcccatc gaacacaatc tggtatccgt gacggtgatt gctccgacgg cgctggaagc
    14221 cgatgcctgg gatactggct tgatggtact cgggccggag aaagccaaag aggttgttcg
    14281 ccgggaaggg ctggcggttt atatgatcac caaagaaggc gatagcttta aaacctggat
    14341 gtcaccacag tttaaaagct tccttgtcag cgaaaaaaat taaagcgcaa gattgttggt
    14401 ttttgcgtga tggtgaccgg gcagactaaa ggctatcctt aaccagggag ctgattatga
    14461 aaaacgccac atgcttaact gacgatcaac gctggcaatc tgtcttagcc cgcgacccga
    14521 atgccgacgg cgaattcgtt ttcgccgtgc gcaccacggg tatcttttgc cgtccttctt
    14581 gccgcgccag acatgccttg cgggaaaatg tctccttcta cgcaaatgcc agcgaggcac
    14641 tcgccgccgg atttcgcccc tgcaaacgtt gtcagccaga caaagccaat ccccggcaac
    14701 atcggttgga taaaatcacc cacgcgtgtc gactgctgga acaggaaacg cctgtaacgc
    14761 tggaagcctt agccgaccag gtggcgatga gtccatttca tctacatcgg ttgtttaaag
    14821 ctactaccgg aatgacgcct aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg
    14881 aatcgctggc gaaaggggag agcgtgacga cgtctattct taacgccgga ttccccgaca
    14941 gcagcagtta ctaccgcaaa gccgatgaaa cgctgggcat gacggctaaa caattccgtc
    15001 acggtggcga aaatctggcg gtgcgttacg cgctggctga ttgtgagctg ggtcgttgcc
    15061 tggtggcaga aagcgagcgg gggatttgcg cgatattgct gggcgatgat gacgcgacat
    15121 taatcagcga gttgcagcag atgtttcccg ctgccgacaa cgcgcctgcc gatctgacgt
    15181 ttcagcaaca tgtgcgtgaa gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt
    15241 taccgctgga tattcgcggc actgcttttc agcaacaagt ctggcaggca ctgcgcacga
    15301 taccttgtgg tgaaaccgtc agttatcagc aactggctaa cgccatcggc aaaccgaaag
    15361 cggtacgggc cgttgccagc gcctgtgccg ccaacaagct ggctatcgta ataccctgtc
    15421 atcgagtggt ccgtggtgat ggcacacttt ccggttaccg ctggggcgtg tcgcgtaaag
    15481 cgcaactgct gcgccgcgaa gctgaaaatg aggagaggta atgttggatc tgtttgccga
    15541 tgctgaaccg tggcaagagc cactggcggc tggtgcggta attttacggc gttttgcttt
    15601 taatgctgcg gagcaactaa tccgcgatat taatgacgtt gccagccagt cgccgtttcg
    15661 ccagatggtc gctccaggcg gatataccat gtcggtggcg atgaccaact gtgggcatct
    15721 gggctggacg acccatcggc aaggttatct ctattcgccc attgatccgc aaacaaataa
    15781 accgtggccc gccatgccac aaagctttca tgatttatgt caacgtgcgg ctacggcggc
    15841 gggctatcca gatttccagc cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa
    15901 actgtcgctg catcaggata aagacgaacc ggatctgcgc gcgccaattg tttctgtttc
    15961 tctgggctta cccgcgattt ttcaatttgg tggcctgaaa cgtaatgatc cgctcaaacg
    16021 tttgttgttg gaacatggcg atgtggtggt atggggcggt gaatcgcggc tgttttatca
    16081 cggtattcaa ccgttgaaag cggggtttca tccactcacc accgactgcc gctacaacct
    16141 gacattccgt caggcaggta aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg
    16201 aagatgttta aactgcgggc tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt
    16261 acttgtctgg cggcagtatc gctggccatt tatcagtgtg atggcgctaa gcctcgccag
    16321 tgcggcatta ggcattggct taattgcttt tatcaatcag cgccttatcg aaacggcgga
    16381 taccagtctg ctggtgttac cggagtttct gggattattg ctgctgttga tggcagtcac
    16441 tctcggatcg caactggcgc tcaccacttt ggggcatcac ttcgtttacc gactgcgtag
    16501 tgaatttatc aagcggattc tggataccca cgtcgagcgc attgaacaac tcggtagcgc
    16561 ctcgttgctg gcgggattaa ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct
    16621 gccggaactg gggcagggga tcattctcac tatcggttca tcggcgtatc tgtggatgct
    16681 gtcgggcaaa atgttgctgg taacggctat ctggatggcg atcaccatct ggggcggttt
    16741 tgtgctggtg gcgcgggtgt acaaacatat ggcgacccta cgtgaaaccg aagacaagct
    16801 gtacacggat tttcaaactg tacttgaagg gcgcaaagag ctgactctga accgggaacg
    16861 cgccgagtat gtgtttaaca acctctacat ccccgatgcg caagagtatc gccatcatat
    16921 tattcgcgca gacaccttcc atcttagtgc cgtgaactgg tcaaacatca tgatgctggg
    16981 cgctatcggc ctggtgttct ggatggcgaa cagtctcggt tgggctgata ccaacgttgc
    17041 tgcgacctat tcgttgacgc tgttattcct gcgtacgccg ctgctttcgg cggttggcgc
    17101 attgccgacg ctgctgacgg cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc
    17161 gcctttcaaa gctgagtttc cgcgcccgca ggcgtttccc aactggcaaa cgctggagct
    17221 gcgtaacgtg acgtttgctt atcaggataa cgcgttttcc gttggtccga ttaatctcac
    17281 catcaaacgt ggcgagctgc tgtttctgat tggcggtaac ggtagcggaa aatcgacgct
    17341 ggcgatgttg ttgacgggct tgtatcagcc acaaagcggc gaaatcttgc tggatggcaa
    17401 acctgtcagc ggcgaacaac cggaagatta tcgcaaactg ttttcggcag tgtttaccga
    17461 tgtctggctg tttgatcaac tgctggggcc ggaggggcaa cccgctaacc cgcaactggt
    17521 tgagaagtgg ctggcgcagc tgaaaatggc tcataagctt gagttaagca acgggcgtat
    17581 tgttaacctg aagttatcaa aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc
    17641 agaagaacgc gatattatcc tgctggatga atgggcggtg gatcaggatc cacacttccg
    17701 tcgtgagttt tatcaggtgt tgctgccgct gatgcaggag atgggtaaaa ctattttcgc
    17761 tatcagtcat gatgatcatt actttatcca cgccgaccgc ctgctggaaa tgcgcaatgg
    17821 gcaacttagc gagctgacgg gcgaagagcg cgatgccgct tcgcgtgatg ccgttgcccg
    17881 gacggcataa tattacgacg ctgacctggc ggactgctgc cgtcaggtca atatgctaaa
    17941 aatcccttca tttctaataa ccctataatt aatcaacgaa attataatgt ttctaaaatt
    18001 agaatataat ttataaacat tatttaaatg ttgttactta agtgttaccg ttgatgccgc
    18061 gcaaatctca cctgcaataa agcgactaaa agtaaggcat taacaagatg aaaaaagtga
    18121 ctgccatgct cttctcgatg gccgtggggc ttaatgccgt ttcgatggcg gcaaaagcga
    18181 aagcgtccga ggagcaggaa actgatgtac tgttgattgg cggcggcatt atgagcgcca
    18241 cgttggggac ctattgacgc gagctggagc ctgaatggtc gatgaccatg gtggagcgcc
    18301 tggagggggg cgcgcaggag agttcgaacg gctagaacaa cgccggaacc gggcattctg
    18361 cactgatgga actgaactac accccgcaaa acgccgatgg cagcatcagt attgaaaaag
    18421 cagtcgccat taacgaagca tttcagattt cccgccagtt ctgggcgcac caggtcgagc
    18481 gcggcgtgct gcgtactccg cgttcattta tcaataccgt tccgcatatg agctttgtct
    18541 ggggcgagga taacgttaat ttcctgcgcg cccgttacgc cgcgttgcaa caaagctcgc
    18601 tgtttcgcgg tatgcgttac tctgaagatc acgcgcagat caaagagtgg gcaccgttag
    18661 tgatggaagg gcgcgatccg caacagaaat tggcactgat gtgaactatg gtgagatcac
    18721 ccgccagtta attgcttcct tacagaagaa atctaacttc tcgctgcaac tcagcagcga
    18781 agtccgcgcc ctaaagcgta atgacgataa cacctggacc gttaccgttg ccgatctgaa
    18841 aaacggcact gcacagaaca tccgcgccaa atttgtcttt atcggcgcgg gcggtgcggc
    18901 gctgaagctg ttacaggaat cggggattcc ggaagcgaaa gactacgccg gtttcccggt
    18961 gggcggacag ttccttgttt cggaaaatcc agacgtggtt aatcgccatc tggcgaaagt
    19021 ttacggtaaa gcatccgttg gcgcaccacc gatgtcggtt ccgcatatcg atacccgtgt
    19081 tctggacggt aaacgcgtag tgctgtttgg gccatttgcc accttctcaa ccaaattcct
    19141 caaaaacggt tcattgtggg atctgatgag ttccaccacc acctctaacg tgatgccgat
    19201 gatgcacgtc gggctggata atttcgatct ggtgaaatat ctggtgagtc aggtgatgtt
    19261 gagtgaagag gatcgttttg aagcgttgaa agagtactat ccgcaagcga aaaaagagga
    19321 ctggcgtttg tggcaagcgg ggcagcgtgt gcagattatc aagcgtgatg ccgagaaagg
    19381 tggcgtactg cgtctgggta ccgaagtggt cagtgaccag caagggacca ttgcggcgct
    19441 gctgggggca tcgccagggg cgtcaaccgc cgcgccgatt atgttgaatc tactggaaaa
    19501 agtatttggc gatcgcgttt ccagcccgca atggcaggct acgttgaaag cgatcgttcc
    19561 gtcttatgga cgcaagctga acggtgatgt agcggcaaca gaacgcgagt tgcagtacac
    19621 cagcgaagtg ctggggctga aatacgacaa accgcaagca gcagatagta cgccgaaacc
    19681 gcaacccgtt caaaaagaag tggcggatat tgcgttgtaa tgatgtgcca catccggcat
    19741 ggtatgccgg atgtggcgta tactgataag acgcgccagc gtcgcatcag gcaaccggct
    19801 cgggcgttag atgttggatg cggcgtaacc gccttatcca accaacgggg tgccgtactt
    19861 cacaacagtt tagcgaactt ccgcgttgtc aattttctct tccgccttcc agacgcggta
    19921 tttcacatct acattgtctg gcgtatacac cacgatcggc agcttgctgt tgtaacgcag
    19981 cattccagca tcgcccagat acgcggtgac aaatttcttc tctttcttgc catccgggca
    20041 ggccatcatc gtggaaaccg gcgagctaac tttatcaaag acataataat catagcccca
    20101 gccttccagc gttttgcttt ccagcttccc gccgagacga tgcaaattgc aatcgacttc
    20161 cagcgtctga ccgattaaca gttctacttt cagggtagat tcatcttctt gcggggttaa
    20221 ctgaatcacc tgacgcttca tgcctgtttc agcttgtgga taaggcgcga ttttttccag
    20281 tggctggacg ctttctgccg cccaggcgga agtggtagcg aaagcggcaa acaatacttc
    20341 aggtagaatg gtcttcattt ttttgctttc cttcttcatt tataggtttt caccgatgat
    20401 gtcgctgaag gttaacatat tcatcagatt tattaatttg ctttatgaat aacttaaaga
    20461 acattcagac aattttacct gctcttttag tgttaaattc tgatgaggga gcaaaccagc
    20521 agaaatatct ctggtgaatt aatcgctata taaatatgtt tataaccatt tgaaatgtga
    20581 gcaaaagccc gtttttgcca cgcttcccgc cactcttttg atcctgctac aggttttacc
    20641 ccgatcgggg tatgcatctt tgacacatcc tttaatatct tagcggctat aaaaatggct
    20701 tattaattat gcggtctatt tggtcgctct caattttcag agcgcgttaa tgatggaagg
    20761 tcaatgtgaa gattgatgca tcccgtcggg gcatactcac tggttgctgg cgcaaagcca
    20821 gtaacggtat ccgtccgccc tggtccggtg atgaatctca ttttctgacc cattgcaccc
    20881 gctgcgacgc ctgtattaat gcttgtgaaa acaacattct gcaacgcggc acgggtggct
    20941 atccgagcgt taatttcaaa aataacgagt gcagcttctg ttacgcctgc gcgcaggctt
    21001 gccccgaatc gctattttct ccgcgccaca ccagggcctg ggatttgcag ttcaccatcg
    21061 gggacgcgtg cctggcgtat cagtcagttg aatgccgccg ctgtcaggat agctgtgagc
    21121 caatggcaat tatctttcgc ccgacgctct ccgggatcta ccaggcgcaa cttaatagcc
    21181 aactttgcaa cggatgtgga gcatgtgcag ccagctgtcc ggtatcagcc ataactgcgg
    21241 agtatctcca tgcacactaa ctggcaagtt tgcagcctgg tcgtgcaggc caaaagcgaa
    21301 cgaatttcag acatcagcac ccaactgaac gcctttcccg gctgtgaagt tgctgtcagc
    21361 gacgcgccga gcggtcagtt gattgtggtg gtggaagcag aagacagcga aacgctgatc
    21421 caaaccattg agtcagtacg caacgtagag ggcgtgctgg cggtgtcgct ggtttatcac
    21481 cagcaggaag agcaaggtga ggaaacacca tgaaactcag tcgtcgtagc tttatgaaag
    21541 ctaacgccgt tgcggccgct gcggcggctg ccggtctcag cgtgccgggc gttgcccgcg
    21601 ccgttgttgg tcagcaggaa gccattaaat gggataaagc gccgtgccgt ttctgcggta
    21661 ctggttgcgg cgttctggtc ggaacgcagc aggggcgtgt ggtggcctgt cagggcgacc
    21721 cggacgcacc ggttaaccgt ggcctgaact gcattaaggg ctatttcctg cccaagatca
    21781 tgtacggtaa agaccgtttg acgcagccgc tgctgcgtat gaaaaacggt aaatatgaca
    21841 aagaaggcga atttacccca atcacctggg atcaggcctt cgatgtgatg gaagagaaat
    21901 tcaaaaccgc cctgaaagaa aaagggccgg aatcgatcgg tatgttcggt tctggtcagt
    21961 ggactatctg ggaaggttat gccgcgtcca agctgttcaa agcgggcttc cgttcgaaca
    22021 acatcgaccc gaacgcacgt cactgtatgg cgtcggcagt agttggcttt atgcgtacct
    22081 ttggtatgga tgagccgatg ggctgctatg acgacatcga gcaggctgac gcgtttgtgc
    22141 tgtggggcgc taacatggcg gagatgcacc cgatcctctg gtcacgcatc actaacagtc
    22201 gtctctctaa ccagaacgtc accgtggcgg tgctttctac ctaccagcat cgtagcttcg
    22261 agctggcgga taacggcatc atctttacgc cgcaatctga cctggtgatc ctgaactaca
    22321 tcgccaacta tatcattcaa aacaatgcga taaatcagga cttcttcagc aagcacgtta
    22381 acctgcgcaa aggggcgacg gacatcggct acggtttacg tccgacccat ccgctggaaa
    22441 aagcagcgaa gaatccgggt tctgacgcct ccgaaccgat gagctttgaa gattacaaag
    22501 ccttcgttgc tgagtatacg ctggaaaaaa ccgctgaaat gaccggcgtg ccgaaagacc
    22561 agttagaaca actggcgcag ctgtatgccg atccgaacaa gaaagtcatc tcctactgga
    22621 cgatgggctt caaccagcat acccgtggtg tgtgggctaa caacctggtc tacaacctgc
    22681 acctgctgac cggcaaaatt tcccagccgg gttgcggtcc gttctccctg accgggcaac
    22741 cttctgcgtg tggtactgcg cgtgaagtgg gcacctttgc tcaccgtctg cctgcggaca
    22801 tggtggtaac taacgagaaa catcgtgata tctgtgagaa gaagtggaat atcccgagcg
    22861 gcaccattcc ggcgaaaatc ggtcttcatg cggtggcaca agaccgtgcg ctgaaagacg
    22921 gcaagctgaa tgtttactgg accatgtgta ccaacaacat gcaggccggg ccgaacatca
    22981 atgaagagcg tatgccgggc tggcgcgatc cgcgcaactt catcatcgtc tccgatccgt
    23041 atccgacagt cagtgcgctg gccgccgact tgatcctgcc gaccgcaatg tgggtagaga
    23101 aagagggcgc ttacggtaac gccgaacgcc gtactcagtt ctggcgtcag caggtacagg
    23161 cgccaggcga agcgaaatcg gatctctggc agttagtcca gttctcccgc cgcttcaaaa
    23221 ctgaagaagt atggccggaa gagctgctgg cgaagaaacc ggaactgcgt ggcaaaacgc
    23281 tgtacgaagt tctgtatgcc acccccgaag tgagcaaatt cccggtatcc gaactggtgg
    23341 aagatcagct gaacgatgaa tcccgcgagc tgggcttcta tctgcaaaaa gggctgttcg
    23401 aagagtacgc atggtttggt cgcggtcacg gtcacgatct ggcaccgttc gatgactacc
    23461 acaaagcgcg cggtctgcgc tggccggtgg tgaatggtaa agaaacacag tggcgttaca
    23521 gcgaaggtaa cgacccgtac gtgaaagcgg gcgaaggcta taagttctac ggtaaaccgg
    23581 atggcaaagc ggtgatcttc gcactgccgt tcgaaccggc ggcggaagca ccggatgaag
    23641 agtacgacct gtggctctct accggacgcg ttctggagca ctggcacacc ggcagtatga
    23701 ctcgccgtgt gccggaactg caccgcgcct tcccggaagc ggtcttgttt attcacccgc
    23761 tggatgcgaa agcgcgcgat ctgcgccgtg gcgacaaagt gaaagtggtt tctcgccgtg
    23821 gcgaagtgat ctcgattgtt gaaacgcgcg gtcgtaaccg tccgccacag ggcctggtgt
    23881 acatgccgtt cttcgacgcc gcacagctgg ttaacaaact gacgctggat gcgaccgatc
    23941 cgctctcgaa agagacggac ttcaagaagt gcgcggtcaa actggagaag gtgtaagcca
    24001 ttatgtcccg gtcagcgaaa cctcaaaatg gtcgccgccg ctttctgcgc gatgtcgttc
    24061 gcacagcagg cgggctggct gccgtgggtg tggcgctggg gttacaacag caaaccgcac
    24121 gcgcatctgg cgtgcggttg cgcccgcccg gagccataaa cgagaacgcc tttgccagtg
    24181 cctgtgtgcg ttgcggtcag tgtgttcagg cttgccctta cgacacctta aaactggcga
    24241 cgctggcctc tggtctgtcg gcgggcacgc catattttgt cgcacgggat attccttgcg
    24301 aaatgtgtga ggacattccg tgcgccaaag tgtgcccaag cggtgcgctg gatcgtgaga
    24361 ttgaatcgat cgacgacgcg cggatggggc tggcggtact ggtggaccag gaaaactgtc
    24421 tcaactttca ggggctgcgc tgcgatgttt gttatcgcga atgcccgaaa attgatgagg
    24481 ccatcacctt ggagctggag cgcaacacgc gtaccggtaa gcacgcccgc tttctgccga
    24541 cggttcacag cgacgcctgt accggttgcg gtaagtgcga aaaagtatgc gtgctggaac
    24601 aaccggcaat caaggtgtta ccgctgtcac tggcgaaagg ggagttaggt caccattacc
    24661 gcttcggctg gctggagggg aacaatggca aatcgtaaac gtgacgccgg gcgcgaggcg
    24721 ctggagaaaa aaggctggtg gcgcagtcac cgttggctgg tgttacgtcg cctttgtcag
    24781 ttcttcgtgc tggggatgtt tttgagcggt ccatggtttg gtgtgtggat cttgcacggc
    24841 aactacagca gtagcctgtt attcgacacc gttccgctga ccgatccgct gatgacgctg
    24901 caaagtcttg ccagtggtca tctgcctgcc acggtggcgt tgaccggtgc ggtgattatc
    24961 accgtgctct acgccctggc gggaaagcga ttattttgca gctgggtctg cccgctgaac
    25021 ccgattaccg acttagcgaa ctggttacgc aggcgctttg acctcaatca gtctgcgacg
    25081 atccctcgcc acattcgcta cgtgctgttg gtggtcattc tggtgggatc agccttaact
    25141 ggcacgctca tttgggaatg gatcaacccc gtttctctga tggggcgtag cctggtaatg
    25201 ggcttcggca gcggcgcgct gcttattctc gcgctgtttt tatttgatct actggtcgtt
    25261 gaacacggct ggtgcgggca catttgtccg gttggcgcgc tgtatggcgt gctgggtagc
    25321 aaaggtgtga ttaccgttgc ggccagcgat cggcagaaat gtaaccgctg tatggattgt
    25381 tttcatgttt gcccggaacc gcatgtgcta cgtgccccgg tgctggatga acaaagcccg
    25441 gtgcaggtca ccagccgcga ttgcatgacg tgcggtcgct gcgtggatgt ctgttctgag
    25501 gatgtattta caataactac acgatggagt tcgggagcga aatcatgaaa agccatgacc
    25561 tgaagaaagc gctgtgtcaa tggacggcga tgctggccct ggtggtaagc ggcgcggttt
    25621 gggcggctaa tggcgtcgat tttagccagt cgccggaagt atccgggacg caggaagggg
    25681 ccattcggat gccaaaagag caggatcgga tgccgctgaa ctatgtgaat cagccgccaa
    25741 tgatcccgca tagcgttgaa ggttatcagg taacgaccaa taccaatcgc tgcttgcagt
    25801 gccacggtgt cgaaagctat cgcaccactg gcgcgccgcg tatcagtcct actcacttta
    25861 tggacagcga cggcaaagtg ggcgcggaag tggctccgcg tcgttatttc tgtctgcaat
    25921 gtcacgtacc gcaggccgat accgcgccaa tcgtggggaa tacctttacc ccatcaaaag
    25981 gttacgggaa ataagaggtt attatgggaa attctgaccg taagcctggt ctgattaagc
    26041 gcctgtggaa atggtggcgt acccccagcc atctggcgct ggggacgctg ctgttgatcg
    26101 gttttgttgg cggcatcgtc ttctgggggg gctttaacac cgggatggaa aaagccaata
    26161 ccgaagagtt ctgcattagc tgccacgaaa tgcgcaacac ggtgtatcag gaatacatgg
    26221 attccgtgca ctacaacaac cgtagcggcg tccgtgcgac ctgtccggat tgtcacgttc
    26281 cgcacgagtt tgtgccgaag atgatacgca agctcaaagc aagtaaagag ctgtatggta
    26341 aaatttttgg cgttattgac acgccgcaga aatttgaagc tcatcgtctg acgatggcac
    26401 agaatgagtg gcggcgcatg aaggacaata actcgcagga gtgccgtaac tgtcacaact
    26461 tcgagtatat ggatacaacc gcccagaaat cggttgccgc gaagatgcat gaccaggcgg
    26521 tgaaagatgg gcaaacctgt attgattgcc ataaagggat cgcgcacaag ctgcccgata
    26581 tgcgtgaagt cgagccaggt ttttaacagg gttattgcgt gggtatgctt gaagccagag
    26641 agttactttg tgagcgggat gaacgaacct tatttagtgg cttgtcattt acgctgaacg
    26701 caggagagtg ggtacaaatc accggtagca acggcgcggg gaagacaacg cttctccgtt
    26761 tgctgacggg gttgtctcgc cctgacgctg gcgaggttct ctggcaaggg cagcccttgc
    26821 atcaggtacg cgacagctac catcaaaccc tgttatggat aggccatcag ccggggatca
    26881 aaacccggct gacggcgtta gaaaatctgc acttttatca tcgcgatggc gatgccgcac
    26941 aatgtctgga agccttggcg caggccgggc ttgccggatt cgaagatatt ccggtaaatc
    27001 aactttctgc cgggcaacaa cgccgcgtcg ctttagcgcg tctgtggttg acccgtgcca
    27061 cattatggat cctcgacgag ccttttacca cgattgacgt taacggcgtc gatcgtctga
    27121 cccagcgtat ggcgcagcat acggagcagg gggggattgt gattctgact acccaccagc
    27181 cgctcaacgt tgctgaaagt aaaattcgcc gcatttcact gacgcaaacg agggccgcat
    27241 gatgttctgg cgtattttcc gtcttgaact gcgcgtagcg tttcgccata gcgccgaaat
    27301 cgccaacccg ctgtggttct tcctgattgt aattaccctt tttccgctca gtatcggtcc
    27361 ggagccgcaa ctgctggcgc gtattgcacc gggcattatc tgggttgctg cgctgctttc
    27421 atccttgctg gcgctggaac gactgttccg tgacgatttg caggacggca gtcttgaaca
    27481 attgatgttg ttgccgttac ccttgcccgc cgttgtgctg gcgaaggtga tggcgcactg
    27541 gatggtaacc ggtctgccgt tactcatcct ttcgccactg gtagcaatgc tactgggaat
    27601 ggatgtttat ggctggcaag tgatggcgct gacgctgctg ctgggaacgc ctacgcttgg
    27661 ctttctcggt gcaccgggcg tggcgctgac agtgggactt aagcgcggtg gtgtgctgct
    27721 cagcatactg gtgttaccgc tgactatccc attactcatc tttgccaccg ccgcgatgga
    27781 cgcggcttct atgcatttgc ccgttgatgg gtatctggca attttaggcg cgttgctggc
    27841 aggcaccgcg acattaagtc cttttgcgac ggcggcagcg ttacgaatca gcattcaata
    27901 acgctggatt actgattttt gttcgagtct ggtatcgaaa ctatgtggaa aacactgcat
    27961 caactggcga tcccaccacg gctgtatcaa atctgtggct ggtttatacc gtggctggca
    28021 attgccagtg tggtcgtgct taccgtcggc tggatctggg gattcggctt tgctccggct
    28081 gattatcagc agggaaatag ctaccgcatt atctacctgc atgtgcctgc ggcgatctgg
    28141 tcgatgggca tttatgcatc aatggcggtg gcggcgttta ttggccttgt ctggcagatg
    28201 aaaatggcca acctggcggt ggcggcgatg gcccccattg gtgccgtgtt tacctttatt
    28261 gccctggtta ccggctctgc atggggaaaa ccgatgtggg gcacctggtg ggtatgggat
    28321 gcacgtctga cttctgaact ggtgctgctg tttttgtatg tgggtgtgat tgccctgtgg
    28381 cacgccttcg acgaccgccg tctggcgggc cgtgcggcag gtatcctggt gctgattggc
    28441 gtggtgaatc tgccgattat tcattactcc gtggagtggt ggaacaccct gcatcaggga
    28501 tcaacgcgga tgcagcaaag tatcgatccg gcgatgcgtt cgccgctgcg ctggtcgatt
    28561 tttggcttcc tgctcctgtc tgccacgctg acgctgatgc ggatgcgtaa tttgattttg
    28621 ctgatggaaa aacgccgtcc gtgggtgagt gaactgatac tgaaaagagg ccgtaaatga
    28681 cccctgcatt tgcttcctgg aatgaatttt tcgcaatggg cggttacgcc ttttttgtct
    28741 ggctggcggt ggtgatgacc gttattccgc tggtggtttt ggtcgtgcac tcggtgatgc
    28801 aacatcgcgc aattctgcgt ggtgtggcgc aacagcgggc gcgtgaggcg cgtttacgtg
    28861 ctgcgcaaca gcaggaggct gcatgaatat tcgccgtaaa aaccgcttgt ggattgcctg
    28921 tgccgtgttg gcagggctag cgctgactat cggtctggtg ctatatgcgc tgcgctcgaa
    28981 tatcgatctc ttttatacgc cgggggaaat tctctacggc aagcgtgaaa ctcagcagat
    29041 gccggaagtc ggtcagcgtc tgcgcgttgg cgggatggtg atgccgggta gcgtgcagcg
    29101 cgatcccaat tcgctgaaag tgaccttcac catttacgat gctgaaggct cagtggatgt
    29161 ctcttacgaa ggcattttgc cggatctgtt ccgtgaaggg cagggcgttg tggtgcaggg
    29221 cgagctggaa aaaggcaatc atatcctcgc gaaagaagtg ctggcgaaac acgacgaaaa
    29281 ctacacgccg ccagaagttg agaaagcgat ggaagctaac caccgtcgcc cggcgagtgt
    29341 ttataaggac ccagcatcat gatgccggaa attggaaacg ggctgctgtg cttggcgtta
    29401 gggattgcgc tgctgctgtc cgtgtatccg ctatggggcg tggcgcgcgg agatgcgcgc
    29461 atgatggcgt cttcccgctt gtttgcctgg ctgctgttta tgtctgtggc tggcgcattt
    29521 ctggtgctgg tcaatgcttt cgtggtcaac gactttaccg tcacctatgt tgccagcaac
    29581 tccaataccc agcttccggt gtggtatcgc gtggcggcta cttggggcgc gcatgaaggt
    29641 tcgcttctgc tgtgggtgct gctgatgagc ggctggacct ttgcggtggc gatttttagt
    29701 cagcgtattc cgctggatat tgtggcccgt gtactggcga taatggggat ggtcagcgtc
    29761 ggctttttgc tgttcattct ctttacctct aacccgttct cacgcacgct gccgaacttc
    29821 ccgattgaag ggcgcgatct taacccgctg ttacaggatc cggggctgat cttccatccg
    29881 cctctgcttt atatggggta cgtgggtttc tcggtggcgt ttgcttttgc cattgcttct
    29941 ttgctgagcg ggcgtctgga cagcacttat gcgcgtttta ctcgcccgtg gacgctggcg
    30001 gcgtggattt tcctgacgct cggcattgtg ctcggttccg catgggccta ttacgaactc
    30061 ggctggggtg gctggtggtt ctgggatccg gtagaaaacg cctcgtttat gccgtggctg
    30121 gtggggactg cactgatgca ctcactggcg gtcactgaac aacgcgccag cttcaaagcg
    30181 tggacattac tgctggcaat cagtgccttc tcgttgtgtc tgttggggac tttcctcgtg
    30241 cgttccggcg tgctggtatc ggtacacgcg tttgcgtctg atccggcgcg cggtatgttt
    30301 atcctcgcct ttatggtgct ggtgattggc ggttcgctgc tgctgtttgc cgcgcgtgga
    30361 cacaaagttc gctcacgcgt aaacaatgcg ctgtggtcgc gggaatcttt gctgttagcg
    30421 aacaacgttt tgctggtcgc cgcgatgctg gtggtgttgc tggggacgct gctgccgctg
    30481 gtgcacaagc aacttggact gggcagtatt tcgattggcg aaccgttctt caacaccatg
    30541 tttacctggc tgatggtgcc gtttgcgcta ctgcttggtg tcggtcctct ggtgcgctgg
    30601 gggcgcgatc gcccacgtaa aatccgcaat ttattgatta tcgccttcat ctctacgctg
    30661 gtgctgtctc tgctgttgcc gtggctgttc gaaagcaaag ttgtggcgat gacggtgctc
    30721 ggcctggcaa tggcctgctg gattgcggtg ctggcaactg cggaagctgc gctgcgtatt
    30781 tcacgcggca cgaaaaccac cttcagttat tgggggatgg tggcggctca cctggggctg
    30841 gcagtgacaa ttgttggcat tgcctttagc cagaattata gcgttgagcg tgatgtgcgc
    30901 atgaagtccg gcgatagcgt cgatattcat gaatatcgct tcaccttccg tgatgtcaaa
    30961 gaggtgactg gcccgaactg gcgcggcggt gtggcgacta ttggcgtaac gcgagagggt
    31021 aaaccggaaa cggtgctgta tgcggaaaaa cgttattaca acactgccgg gtcgatgatg
    31081 accgaagcgg caattgacgg cggcatcacg cgtgacctgt acgccgcgct cggtgaagag
    31141 ctggaaaacg gcgcgtgggc cgtgcgtctt tactacaaac catttgttcg ctggatttgg
    31201 gcgggcgggc tgatgatggc gttgggcgga ctgctgtgtc tgtttgatcc tcgctatcgt
    31261 aagcgcgtga gtccgcaaaa aactgcgccg gaggccgtat gaagcgcaaa gtattgttaa
    31321 ttccgttgat tatcttcctg gcgattgccg cggcgctgct gtggcagctg gcgcgtaatg
    31381 ccgaagggga tgatccgacc aatctggaat cggcgctcat tggcaagcct gtgccgaagt
    31441 ttcgtctcga atcactggac aatccggggc agttttatca ggcggatgtg ctgactcagg
    31501 gcaaaccagt actgcttaac gtctgggcga cttggtgtcc gacctgccgt gcggaacatc
    31561 aatatctgaa tcagctttca gcgcagggca ttcgcgtggt cggcatgaac tataaagacg
    31621 atcgccagaa ggcaatcagc tggctgaaag agctgggcaa tccttacgcg ctaagcctgt
    31681 ttgatggcga cggcatgtta gggctggatc tcggtgtcta tggcgcgcca gaaaccttcc
    31741 ttattgacgg caacggtatc attcgctatc gccatgcggg cgatctcaat cctcgcgtct
    31801 gggaagaaaa gatcaagccg ctgtgggaga aatacagtaa ggaggccgca caatgaggtt
    31861 tttattgggc gtgctgatgc tgatgatctc cggctcagcg ctggcgacca tcgacgtgtt
    31921 gcagtttaaa gatgaagcac aggaacaaca gttccgtcag ctcactgaag aactgcgctg
    31981 cccgaaatgc cagaacaaca gcattgccga ttccaactcg atgattgcca ccgacctgcg
    32041 tcagaaagtg tatgaactga tgcaggaagg taaaagtaaa aaagagattg tcgattatat
    32101 ggtggcgcgt tacggcaact tcgtcactta cgatccgccg ttaacgccgc tgaccgtgct
    32161 gctgtgggtg ctgccagtag tggctattgg cattggcggt tgggtcatat acgcccgctc
    32221 gcggcgtcgg gtacgcgtgg tgccggaagc gtttcctgaa caaagcgtgc cggaaggtaa
    32281 gcgtgccgga tatgttgttt atctgccggg tattgtggtg gcgttaattg tggctggcgt
    32341 cagctactac cagactggca attatcagca ggtgaaaatc tggcagcagg ccacggcaca
    32401 ggctccggcg ttgctggaca gggcgctgga cccgaaagcc gatccgctca acgaagaaga
    32461 gatgtcgcgc cttgcgctgg ggatgcgtac tcaactgcaa aaaaatccgg gagatataga
    32521 aggctggatt atgttgggcc gcgttggcat ggcgctgggt aacgccagta tcgccaccga
    32581 tgcatacgct actgcgtatc gcctcgatcc gaagaacagc gatgccgcac tgggatacgc
    32641 tgaagcgttg actcgttcat ctgatcccaa cgacaaccgc ctcggcggtg aactgctacg
    32701 tcagctggtg agaacggacc atagcaatat ccgtgtgcta agcatgtatg cgtttaatgc
    32761 ctttgagcag cagcgatttg gcgaagccgt tgccgcgtgg gagatgatgt tgaaactctt
    32821 acctgccaac gatactcgcc gtgcggtgat tgaacgtagt atcgcgcagg cgatgcaaca
    32881 tttgtcgccg caggagagta aataaaaaga ggcccgatgg tgatgccatc gggctagact
    32941 gatgacaaag tgcgcttagt ttatgccgga tgcggcgtga acgccttatc cggcctacaa
    33001 aattgtgtaa attcaataaa ttgatcgatc cacgtaggcc tgataagcgt agcgcatcag
    33061 gcagttttgc gtttgtcata aatctcagcc cgatgataaa tccatcgggc tatttttatt
    33121 gtgccccgcg ttgttgcagg aacagaatgg tggccgccac gcgtgagcgg acattgagtt
    33181 tacgcagcag attgcgaata tgtactttta ctgtctgctc ggaaatattc aacaccgatg
    33241 caatctgttt atttgacagc ccctgtgcca gctcgtgcag aacatccagt tcgcgctccg
    33301 tcagcacgct gaagggatct tcttccgcgc caaacatttc acgttcacgt aagtactgat
    33361 tgacgcgttc gctaaagact ttgctgcctt tcgctccggt acgaatcgct tccagcaata
    33421 cttccgggtc gctgtctttc aacagataac cgtctgcgcc tgcgtctatc agcgcaaaga
    33481 catcgctgga ggcatcggat acggtcagga taataatttg cgcggtaacg ccatccctgc
    33541 gcaaggcatt gagggtatcc aggccactca tacctttcat attgagatcc agcaagatca
    33601 cgtcgatatc cagtctattc gccagatcga tcgcgctcgc gccttcgccc gcttcggcga
    33661 ccacttcaaa gccaggatca agctccagta actgacgaac accgcgtcgc ataagtggat
    33721 gatcatccac aatcatcacc tgaaaaggcg ttgcttcagg catagtagtc tcctgaggtt
    33781 ttattagaat cattatgttt gagcgtagcg ttggtaacgc taccaggcgc gtgaatgtta
    33841 ccccatttta tgtatggaca tgtagtgaaa actacaacct tgtcgttcgc tttggcatca
    33901 tttttattat aaagacggaa ttttgcatca gcttctcatc aagaaaaaca actaaaaaat
    33961 caatcacaat acttgtgcat ttctttaaac agtttttaaa aactgactcc gggtatggag
    34021 ctatgggtat tttctgtacc caatgctttt aactgcaatt aatttcatag gatgaaagct
    34081 caatgcatca atctggttct gtttctcttt gtcgttccgc aatatctgtt ctggtggcta
    34141 cagcgttata ttcacccata gcattggcat caacagttga gtatggtgag acagttgatg
    34201 gtgttatcct ggaaaaagat atccagctgg tttatgggac cgccaataat acgaaaatca
    34261 atcctggcgg agaacagcat ataaaagaat ttggtgtaag tagtaatact gaaattaacg
    34321 gagggtatca atacattgaa atgaatggta ccgcagaata ctcagtatta aatgatggtt
    34381 atcaaattgt tcaaatgggt ggcgcggcaa accagactac tctcaataat ggtgtgctac
    34441 aggtttatgg cccagcgaat gataccacga ttaaaggcgg gcgcttaatc gttgaaaaag
    34501 atggggggac cgtttttgcc gctatcgaaa agggaggact actggaggtt aaagaggggg
    34561 gatttgcgct tgcggtagat cagaaagcag gcggtgctat taaaacaacc acgcgggcca
    34621 tggaggtatt cggaacaaac cgtctcggtc agttcgatat caagaatggt attgctaata
    34681 atatgttgtt ggaaaacggc ggaagtttgc gagttgaaga aaatgacttc gcttataata
    34741 ctactgtgga tagtggcggc ttactggagg ttatggatgg cgggactgca actggcgttg
    34801 ataaaaaagc aggcgggaaa ttaattgtct caacgaatgc gctggaagtg agtggtacaa
    34861 acagtaaagg ccaatttagt ataaaggatg gtgtgtcaaa aaattatgaa ctggatgatg
    34921 gttccgggct cattgttatg gaggacacgc aggccattga cactatcctt gatgagcatg
    34981 ccactatgca atcgctggga aaggatactg gtacgaaagt gcaggcaaat gcggtatatg
    35041 atctcggtcg atcagaccag aatggaagta tcacgtattc ctcaaaagcc atctctgaaa
    35101 atatggtaat caacaatggc cgcgctaatg tctgggctgg cacaatggtt aacgtgtcag
    35161 tcagagggaa tgatggcatt cttgaggtca tgaagccgca aataaattat gcacccgcta
    35221 tgttggtggg taaggtagtg gtttctgagg gcgcttcttt tagaacgcat ggtgccgtgg
    35281 ataccagcaa agcggacgtt tcgctcgaaa atagcgcatg gaccatcatt gccgatatca
    35341 ctacgacgaa ccaaaacact cgcctcaact tagccaacct tgcgatgtcc ggcgcaaatg
    35401 tgattatgat ggctgagcca gtgactcgtt catctgtgac ggcaagtgcg gaaaatttca
    35461 ttacgttgac caccaatacc ctgtcgggaa acggcaattt ttatatgcgt accgatatgg
    35521 ccaatcatca gagcgatcag ctcaacgtca ccggtcaggc aacaggtgat ttcaaaatat
    35581 tcgtgacgga caccggtgcc agcccggcag caggagatag ccttacactg gtaacaacgg
    35641 gcggcggtga tgctgcattt acgttgggca atgccggagg cgttgttgat atcggtacgt
    35701 atgaatatac cttgctggat aatggcaacc atagctggag cctcgcagag aatcgcgcgc
    35761 aaattacccc ttcaaccact gatgtgctga acatggcagc cgcacaaccg ctggtatttg
    35821 atgcagaact ggacaccgtg cgtgagcgtc ttggtagcgt aaaaggcgtt agttacgata
    35881 cggtgatgtg gagttcggca attaacaccc gcaacaacgt gaccactgat gcgggagctg
    35941 gttttgagca aacattgacg ggcctgacgc tcggtatcga tagccgtttc tcccgtgaag
    36001 aaagcagtac aattcgcggc ttgttctttg gttactctca ttctgatatt ggttttgatc
    36061 gcggcggaaa aggtaatatc gatagctata ccctgggggc ttatgccggt tgggagcatc
    36121 agaacggtgc ctatgttgat ggggtggtga aagttgaccg ttttgccaac accatccatg
    36181 gcaagatgag taatggggca acagcgtttg gcgattacaa tagtaacggc gcgggtgctc
    36241 atgttgagag cgggttccgt tgggttgacg gattgtggag tgttagaccc tatctggcct
    36301 ttaccggctt taccacagat ggtcaggact acacgttatc aaacggcatg cgcgcggatg
    36361 tgggaaatac ccggatatta cgcgctgaag cgggaacggc ggtaagctat cacatggacc
    36421 tgcaaaacgg tacgacgctg gaaccctggc tgaaagccgc cgtgcgtcag gaatacgccg
    36481 attctaacca ggtgaaagtt aatgacgatg gcaaatttaa taatgatgtg gctggaacca
    36541 gtggcgttta tcaggctggg ataaggtcat cgtttacccc gacgttaagc ggtcatttgt
    36601 cagtcagcta tggcaatggc gcaggggtag aatcgccgtg gaatactcag gcgggtgtgg
    36661 tctggacgtt ctgataacag aaaataaaca ggctgtgatg tgtcacggtc tgtttatcga
    36721 attaattgca gatataaaaa aaccaaccgt aagggttggt tttttcttgg gatttttggt
    36781 cacgagagta gtttaattga gcctgtaact gcatgatttt aaatatgtag attaattgag
    36841 ttttgaaatc gtatatccaa acgtataact aaagtgattt gctgcaaaaa tgtacagctt
    36901 taaccgttat cattttccgg ctatccgcta tgaagctggc tggtggatga tcacggataa
    36961 cctgacatct acagccgcct taaaaagctg aacccgacaa gcatatcggc atcatgtcca
    37021 ccagcgtgac gcattttttt aattgcgcgg gcgtaaaaat ttgacggggc ggacagttcc
    37081 ataagtaatg gattgcagga atttagcccc cctcctgaat gcaattttcg tatatatata
    37141 tacaaaacta cctgtcccac ctgtcccacc tgtcccacta gcacttaata gattgatttt
    37201 gttagaaatg aattaggtaa ggtgggacaa gtccaggctg ttttgggaca agttgggggg
    37261 aagtgggaca agtcgagcaa gtgaatcaag ccgtgttagc gcggtaaacc gactgtgctg
    37321 ccgcaatttt gcgttgtcgg gagggtactg taacagccac catacgcgca caatcttcgg
    37381 tggtaacgca atccagacgt gcgcaacatc cccagacagg taaatcatta cctagcacag
    37441 ctaattgcgc cagaatcgat tacagcgcgt ttctgtggca ttcaagaacg gctatcacta
    37501 gtgtattgat atgtaatccg catatgagtt taagcaacaa aatgatccct tttcgaaaaa
    37561 ggcactaacc gttttttccg caatgatccg ttggtgattt tgtaacttat tgttttgtat
    37621 gtatgatttt aaatttggta gggttataaa taggggatgg ttttgtcaag aattgcaaac
    37681 tatgcttatt tattgctctt ggttcgtttg ttttctgttc gttaatgggg gatcattttt
    37741 cactttttgg gggatcattt cggagggggt ggggctggca acacgccagc accctcgatg
    37801 cgtttgtgtt tagcagtact cccagtctgt ggctagtaga ctctcaggag atagcgtaac
    37861 gctatcctct gtgacgggat tcaacgcaat caaatcgttt ccgcgccgtt cgagaaagac
    37921 gatgtacgga agatgccagg cactgcggcg agctggcacg cgtcggcctg gctgcaactt
    37981 gtcgataata tccaccagcg aaacgttcat gtgattcaaa aaactcctga aacgctctac
    38041 aactcaataa cacaaaaaat aatgcagaac tgccactata aaaccttgtc tgtctggtag
    38101 ttccgccaga cagattttgc gatttctatc cccatatccg cctgtgtgcg gtttgctgcc
    38161 tctctcaagc ccccgaaatt atccagtatg acgggcgaac gtgctgcctt gtggatacat
    38221 tccgcgcgac gttttactgc ctgttcgcgc tccttatcgg tgttcacctg ttgcatgacc
    38281 tcagcccacc gggccgccgc tctccggtac aggcctttag actccagatc ttccgcttta
    38341 ctgtcgtgaa tcatactcat acgccagctt gctctttgtt cgacttacgt ttgcgcttct
    38401 ctttcagtgc catcagtcgt gcttccgcgt tctgctgttc ctgttgtgtc acctcgccgc
    38461 atggctggct attcagatcg taacgcacgc caccagccat taaggcacgg taataacgcg
    38521 gagaatgagc ataagatgct agcgctgcac gtaatgcccc tgacccaaaa gcctgcgccc
    38581 tgacggcaat atcctgtatc aggtcttcaa atatccctac cttaagcggc ttcggtgatt
    38641 ccaggctgaa taattcaggc catagttcaa taagacggtt agcgcgcctg cggtttttgc
    38701 cctggcgttt ggtcatatgc cgccatggtg tcgcccctgt gggcttctgt tggcggctct
    38761 gcggtgcgtt ctgattgccg ggtatcactt tatgcgctga tgtggtttta tcctgctgtg
    38821 tcgtctgtgt ctttttcttt ggtatgccgt aaattgaatc gccacggata atctagacac
    38881 ttccgagccg ttgataatac tggttttcat attctgtcgg tgacatctgt tcgctagaac
    38941 catgccgacg cttactgaat cgccccggga atcctggaga ctaaactcct tgagagagag
    39001 gcaaacagga tgactaaaaa tatgcgcttt tcccctgagg tccgtcagcg ggccgttcgt
    39061 atggttatcg aaagccagaa tgactatgac tcgcagtggg cggccatcag ctccatcgcc
    39121 cctaaaatcg gctgtacgcc tgagaccctg cgtacctggc tgcgtcagta tgagcgcgat
    39181 gccggtgcag gtgacggcgg actgagcacc actgaacgcc agcgtctgaa ggaactggag
    39241 cgtgaaaacc gcgaactgcg tcgcagcaac gatatcctgc gccaggcttc cgcgtatttt
    39301 gcgaaggcgg agttcgaccg cctgtggaaa aaatagcgcc tctggtggat accctgcgtg
    39361 gtacgcacgg agtcggaccg gtatgccacg agctggctgt cgccccgtca acgtattacc
    39421 ggcatcgtgc gcatcaacgg catccggata aacgcagtca gcgggcacag cgtgatgacc
    39481 tgctgaagcc ggagatacag cgcgtgtacg atgaaaacca cagcgtgtac ggtgtgcgca
    39541 aggtctggcg ccagttgttg cgtgaggagg tgaccgtggc caggtgcaca gtagaacggc
    39601 tgatgaaggt gatggggctt gccggtgtgc gccggggtaa aaaggtccgc accacagtca
    39661 gccggaaaga agccgctgcg ggtgaccggg tgaaccgcca gttcgtggca gaatgcccca
    39721 accagctgtg ggtggctgac ttcacgtatg tgagcacctg gcagggcttc gcttacgtgg
    39781 cgttcatcat cgatgtgttc gccggcgtca ttgtgggctg gcgggtctca tcgtcgatga
    39841 aaacgacgtt cgtgctggat gcactggagc aggcgctgtg ggcccgtcgc ccgtcgggca
    39901 ccatccatca ctcagataag gggtcacaat atgtgtcgct ggcatacact cagcggctta
    39961 aggatgcaga gttgctggcg tcaaccggca gcacaggcga ctcatatgac aacgcgatgg
    40021 cggagagcat caacggactt tataaagcgg aggtgataca tcgtaagagc tggaaaaacc
    40081 gcgccgaagt ggaactggcc acactgacgt gggtggactg gtacaataac cgacggttgc
    40141 tgggacgact gggtcatatc ccgccggcag aagcggaaaa ggcatattac gcttccatcg
    40201 gaaatggtga tctggcagcc tgagttcaca gacaaaacac tctcccggaa aacaggggcg
    40261 gttcattgcg atgtagagcc atatgatttg atcgacaaag acccgggcat gcaaatgtcc
    40321 ttgtctttgt cgttatctga caggatcaat caccgcctcc ccaggaggtg gttttggggt
    40381 gacaacaaat atggtaaaaa attaactttt attgttaatc atttccattt ccatttccat
    40441 ttccatttcc tttttaaagc aaataacaat atggtaagta ttgtgcatat tgatactatg
    40501 aaagatatta atacgacctg tttgaaccta taagttgtgt caatacgtat aatataatta
    40561 cctttagtca attccgcttt gaaatagccg tctctcgaat atttttttgc cccatctaat
    40621 tctaatgatt tataagacat gaaaggtatg ctgcaagtgg tttttgttgg aatactaaat
    40681 gtgaaaacag gtaacccatt tgaacctaag gttcgtgaga aagcgtatga cagcccatta
    40741 tcgcatttta attctttaat ccggtcacca agctctggtg catttgatag aacataatct
    40801 ttataatttg cactggcggc aatatcaatt gtgaggttgt ggctactttt tttaggtaca
    40861 gtaatcgcac ttgtcatcaa acatgttgat gtgaaaatta acaagatata ttttaatttg
    40921 attcttcctg atgcatggag aataagtaaa atgctaaata gcaggaatgg aataaaacgc
    40981 caagtaaatt gaatttttga taaatgtgta aatacgccac ctagaaaacc aaaattaatg
    41041 ccaccacaaa tgattagcaa tataattaaa acaagcaggt aatcttgttt ctttttattg
    41101 tgatctgtcg caaagttcca tataaatgca aaggtaattg gcagtccgat ccctaatgcc
    41161 attcccttta gggggcctga ttttattatt tctccagaaa taagatcgta aagtgatatg
    41221 tatgttcgtg acattgttgg gaaccagtca cgatctaaca tagta
//
LOCUS       DAECNO010000027        40825 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.027, whole genome shotgun sequence.
ACCESSION   DAECNO010000027 DAECNO010000000
VERSION     DAECNO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.027"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            267..608
                     /locus_tag="KKO08_001451"
     CDS             267..608
                     /locus_tag="KKO08_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBD6317753.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(599..1525)
                     /locus_tag="KKO08_001452"
     CDS             complement(599..1525)
                     /locus_tag="KKO08_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6317754.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1615..2613
                     /locus_tag="KKO08_001453"
     CDS             1615..2613
                     /locus_tag="KKO08_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBD6317755.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2610..2828)
                     /locus_tag="KKO08_001454"
     CDS             complement(2610..2828)
                     /locus_tag="KKO08_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBD6317756.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2830..4845)
                     /gene="ligA"
                     /locus_tag="KKO08_001455"
     CDS             complement(2830..4845)
                     /gene="ligA"
                     /locus_tag="KKO08_001455"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBD6317757.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4916..5902)
                     /gene="zipA"
                     /locus_tag="KKO08_001456"
     CDS             complement(4916..5902)
                     /gene="zipA"
                     /locus_tag="KKO08_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBD6317758.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6132..6893
                     /gene="cysZ"
                     /locus_tag="KKO08_001457"
     CDS             6132..6893
                     /gene="cysZ"
                     /locus_tag="KKO08_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBD6317759.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7078..8049
                     /gene="cysK"
                     /locus_tag="KKO08_001458"
     CDS             7078..8049
                     /gene="cysK"
                     /locus_tag="KKO08_001458"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBD6317760.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8433..8690
                     /gene="ptsH"
                     /locus_tag="KKO08_001459"
     CDS             8433..8690
                     /gene="ptsH"
                     /locus_tag="KKO08_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBD6317761.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8735..10462
                     /gene="ptsI"
                     /locus_tag="KKO08_001460"
     CDS             8735..10462
                     /gene="ptsI"
                     /locus_tag="KKO08_001460"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBD6317762.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10503..11012
                     /gene="crr"
                     /locus_tag="KKO08_001461"
     CDS             10503..11012
                     /gene="crr"
                     /locus_tag="KKO08_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBD6317763.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11054..11905)
                     /gene="pdxK"
                     /locus_tag="KKO08_001462"
                     /gene_synonym="thiJ"
     CDS             complement(11054..11905)
                     /gene="pdxK"
                     /locus_tag="KKO08_001462"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBD6317764.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12010..12384
                     /locus_tag="KKO08_001463"
     CDS             12010..12384
                     /locus_tag="KKO08_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBD6317765.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12417..13140
                     /locus_tag="KKO08_001464"
                     /pseudo
     CDS             12417..13140
                     /locus_tag="KKO08_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13344..14255)
                     /gene="cysM"
                     /locus_tag="KKO08_001465"
     CDS             complement(13344..14255)
                     /gene="cysM"
                     /locus_tag="KKO08_001465"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBD6317766.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14389..15486)
                     /gene="cysA"
                     /locus_tag="KKO08_001466"
     CDS             complement(14389..15486)
                     /gene="cysA"
                     /locus_tag="KKO08_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBD6317767.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15476..16351)
                     /gene="cysW"
                     /locus_tag="KKO08_001467"
     CDS             complement(15476..16351)
                     /gene="cysW"
                     /locus_tag="KKO08_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBD6317768.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16351..17184)
                     /gene="cysT"
                     /locus_tag="KKO08_001468"
     CDS             complement(16351..17184)
                     /gene="cysT"
                     /locus_tag="KKO08_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBD6317769.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17184..18200)
                     /gene="cysP"
                     /locus_tag="KKO08_001469"
     CDS             complement(17184..18200)
                     /gene="cysP"
                     /locus_tag="KKO08_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBD6317770.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18504..19295)
                     /gene="ucpA"
                     /locus_tag="KKO08_001470"
     CDS             complement(18504..19295)
                     /gene="ucpA"
                     /locus_tag="KKO08_001470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBD6317771.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19482..20282)
                     /gene="murR"
                     /locus_tag="KKO08_001471"
     CDS             complement(19482..20282)
                     /gene="murR"
                     /locus_tag="KKO08_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBD6317772.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20446..21342
                     /gene="murQ"
                     /locus_tag="KKO08_001472"
     CDS             20446..21342
                     /gene="murQ"
                     /locus_tag="KKO08_001472"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBD6317773.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21346..22770
                     /gene="murP"
                     /locus_tag="KKO08_001473"
     CDS             21346..22770
                     /gene="murP"
                     /locus_tag="KKO08_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBD6317774.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22950..23849)
                     /gene="yfeX"
                     /locus_tag="KKO08_001474"
     CDS             complement(22950..23849)
                     /gene="yfeX"
                     /locus_tag="KKO08_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBD6317775.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23945..24520)
                     /locus_tag="KKO08_001475"
     CDS             complement(23945..24520)
                     /locus_tag="KKO08_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBD6317776.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24581..25030)
                     /locus_tag="KKO08_001476"
     CDS             complement(24581..25030)
                     /locus_tag="KKO08_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBD6317777.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25017..25442)
                     /locus_tag="KKO08_001477"
     CDS             complement(25017..25442)
                     /locus_tag="KKO08_001477"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD6317778.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25656..26525
                     /gene="amiA"
                     /locus_tag="KKO08_001478"
     CDS             25656..26525
                     /gene="amiA"
                     /locus_tag="KKO08_001478"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBD6317779.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26529..27428
                     /gene="hemF"
                     /locus_tag="KKO08_001479"
     CDS             26529..27428
                     /gene="hemF"
                     /locus_tag="KKO08_001479"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBD6317780.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27434..28486)
                     /gene="eutR"
                     /locus_tag="KKO08_001480"
                     /pseudo
     CDS             complement(27434..28486)
                     /gene="eutR"
                     /locus_tag="KKO08_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28532..29032)
                     /gene="eutK"
                     /locus_tag="KKO08_001481"
     CDS             complement(28532..29032)
                     /gene="eutK"
                     /locus_tag="KKO08_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBD6317781.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29045..29704)
                     /gene="eutL"
                     /locus_tag="KKO08_001482"
     CDS             complement(29045..29704)
                     /gene="eutL"
                     /locus_tag="KKO08_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBD6317782.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30886..31677
                     /locus_tag="KKO08_001483"
     CDS             30886..31677
                     /locus_tag="KKO08_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317783.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32397..32792
                     /locus_tag="KKO08_001484"
     CDS             32397..32792
                     /locus_tag="KKO08_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD6317784.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32810..33073
                     /locus_tag="KKO08_001485"
     CDS             32810..33073
                     /locus_tag="KKO08_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317785.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            33096..33638
                     /locus_tag="KKO08_001486"
     CDS             33096..33638
                     /locus_tag="KKO08_001486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317786.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33777..34064
                     /locus_tag="KKO08_001487"
     CDS             33777..34064
                     /locus_tag="KKO08_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317787.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            34103..34309
                     /locus_tag="KKO08_001488"
     CDS             34103..34309
                     /locus_tag="KKO08_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317788.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34621..35106
                     /locus_tag="KKO08_001489"
     CDS             34621..35106
                     /locus_tag="KKO08_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD6317789.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            35087..35365
                     /locus_tag="KKO08_001490"
     CDS             35087..35365
                     /locus_tag="KKO08_001490"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317790.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35358..36818
                     /locus_tag="KKO08_001491"
     CDS             35358..36818
                     /locus_tag="KKO08_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6317791.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36852..37661
                     /gene="murI"
                     /locus_tag="KKO08_001492"
     CDS             36852..37661
                     /gene="murI"
                     /locus_tag="KKO08_001492"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD6317792.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37780..38474)
                     /locus_tag="KKO08_001493"
     CDS             complement(join(37780..38228,38228..38474))
                     /locus_tag="KKO08_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6317793.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(38993..40366)
                     /gene="eutA"
                     /locus_tag="KKO08_001494"
     CDS             complement(38993..40366)
                     /gene="eutA"
                     /locus_tag="KKO08_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBD6317794.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40341..40820)
                     /gene="eutP"
                     /locus_tag="KKO08_001495"
     CDS             complement(40341..40820)
                     /gene="eutP"
                     /locus_tag="KKO08_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBD6317795.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        10329 a        10512 c         9907 g        10077 t
ORIGIN      
        1 agcgccctaa aggcgctttt ttgctatctg cgatactcaa agattcgaac ctgcagcagg
       61 tttgagttga gcgcagcgaa acaacggagc cgctcgcggc gacggcccga aaggcgagcg
      121 aagcgagtca tcctgcacga cccaccaatg caaaaaagcg ccctaaaggc gcttttttgc
      181 tatctgcgat ttgtaagatt gatttttctt ctcaagttcc ttgttcttct accgatatta
      241 cctcgtacag tgacgaggac tgaacaatgg ccactatctc ttcaacttca attccttcca
      301 tacaaacaca gagtagtaac agggcatcac aaggtagcga tgttgcttca caaatcgcgc
      361 ggataagtca gcaaatcatc aaactcactc aacaaattaa agagattgta gacactagcg
      421 gcagtgctga agacaagcaa aaacaggccg aattgattca acagcaaatc accctactgg
      481 aaacacaact ggcgcagttg cagaaacagc aggcagaaaa ggcgcaggag aaagagcagc
      541 gtttgtctct gaatgtatcc cttctaaatc ctgttgaaaa aacgactcat atcgatatct
      601 atatctgata caacggatcc ccttcccgac cagccattag cgcattgccc tggtctcgta
      661 ccacgtccca taacgcttca gcggcggtgg aaagtgaacg gttcttcctg cgcaccagca
      721 tcaactggcg ttcaaccacc ggcgtaatgc gcttaaccac cagtgggcta ccttcaggca
      781 atggtagcgc cagtgcagga agaatactga tgccgatgcc tgcggctacc atcgggaaca
      841 gcgtcgccgg atgtccaatc tcctgcacaa tattcgcctg aataccattg cgcgccagcg
      901 ctgcgtcaat cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat ttcgctccct
      961 gtaatgcctg ccagggtaca taatcttcca cggccaaagc gctatcgcga tggcaaagca
     1021 gaaagaaagg ctcggaaaga atcgcttcac attgcagatc gcccactggg cctggatcga
     1081 tgacgatgcc aaagtcgaca tctccctggc gaatactttc catcacccac tgctgcggac
     1141 gatcatgcag gacaaactga atatctggat agcggcgatg gctttcagca atgcattgcg
     1201 gaataagatg cgcggaaatg gtctggctgg cagcgacccg aacttttccg ctcagttgtt
     1261 gccccatacg cccggtatcg cgcaacgtgc tgttaagttc atccagcagt cgctcaagac
     1321 gcaaagccaa ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt ctgtccagca
     1381 ggcgaacacc agtatgattt tccagctcct tcacactgtg acttactgcc gactggctca
     1441 agccgatacg ctctcctgca cgactaaaac ttttctcctg cgctactgtg acgaaaacct
     1501 ttaattgttt taaagaataa ttcatctgtt ttcttcatga atggatgcaa taaatcaatt
     1561 ttatttctca aactggaaga agcacaatag aaccatcgat catctggagt ctttatgaaa
     1621 ctttttcgta tcctcgatcc tttcacctta accctgatca cggtggtgtt gctggcctct
     1681 ttctttccgg ccagaggcga tttcgtcccc ttctttgaaa atctgaccac cgcagctatt
     1741 gccctgctgt tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat tgctggcggt
     1801 ggtcactggc gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt tccgattctg
     1861 ggtgtactgt ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta ctccggtttt
     1921 ctctacttgt gcattctccc ggctaccgtg cagtctgcaa tcgccttcac gtcaatggcg
     1981 ggcggtaacg tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct ggggattttc
     2041 ctttcaccat tgctggttgg tctggtgatg aatgttcacg gtgcaagggg cagccttgag
     2101 caggtcggta aaattatgct gcaactgctg ctgccgtttg tgttggggca tctttcccgg
     2161 ccgtggattg gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac tgaccagacg
     2221 tccattctgt tggtggttta tacagcgttc agcgaagccg tcgttaatgg tatctggcat
     2281 aaagttggct ggggatcatt gctgtttatc gtggtggtca gctgcgttct tctggctatc
     2341 gtgattgtag ttaacgtctt tatggcacgc cgactgagct tcaataaggc agatgaaatt
     2401 actatcgtct tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat ggcaaacatt
     2461 ctgttcccca catcggtgat cggtatgatg gtgctgcccc tgatgatttt ccatcagatc
     2521 caattgatgg tctgtgcggt gctggcgcgt cgatacaaac gccagaccga acagttacag
     2581 gcgcagcagg aaagcagcgc cgataaagct taaagcggac gcttcagggg ctggaccagt
     2641 tgcgtcagcc cctcggtttt aatcagcagc gtgatttgca ttagctcacc gagcttccct
     2701 gccgggaact catctttgcg ggcaaaccac agcagatact cctccggcag gtcaattaag
     2761 cgacgccctt tgtatttgcc aaacggcatt atcgtattgg ctatttcaat cagctgctct
     2821 ttttccatct cagctaccca gcaaacgcag catttccgct tcgtcgatga cttcaatgcc
     2881 cagttcctgc gccttcgcca gtttagatcc tgcagcttca cccgctatca ccagatcggt
     2941 tttcttcgac acgctgcccg cgactttcgc gcccagttcg accagtcgag ctttagcgtc
     3001 atcacgcgac atctggctta agctgcccgt aagcaccacg gttttaccag caaacgggct
     3061 gtcaatctct tccgcgttga taacgatcgg cgcaggccag tgaacacctt ccgccaacag
     3121 ctcgctgatg acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt gggatgcaac
     3181 gacaatgcca acatcaggca ccttttgcag ctcttcaatc gaagcggctt ccagcgcttc
     3241 cagcgtgccg aaatatgccg ccagacctgc tgcggtggcc tcgccgactt cacggatgcc
     3301 aagtgcatag aggaagcgag caaaggtggt ttctttcgct ttttccagcg cgttaaccac
     3361 gttttgtgcc aattttggcc ccatacgctc cagtccggtc agtttgcctg cggtgagttt
     3421 gaacagatct gccggagtat ggacatattc tttttcaacc agctgatcga tgattttgtc
     3481 gcccattccg tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg actctttacg
     3541 ctgcgcaccg caaatcaggc caccggtaca gcgggcaacc gcttcacctt ccacacgctc
     3601 aacgtcagaa ccacataccg gacaatgcgt cgggaataca acctcacggg tatcttccgg
     3661 gcgttcagaa agcacgacgt taaccacctg cgggatcacg tcgccagcgc ggcgaatcac
     3721 cactttatcg ccaatgcgta aaccaagacg ttcgatttca tccgcattgt gtaaggttgc
     3781 gttactcacc agcacgccag caacatggac aggttccaga cgcgcaacag gcgtaatcgc
     3841 gccagtacgc ccaacctgaa actcgacgtc acgcacaaag gtcatctgct cctgcgccgg
     3901 gaatttaaac gcaaccgccc agcgcggggc acgcgcgaca aagccaagct gctcctgctg
     3961 ttccagtgag ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg tcgggcggtc
     4021 ttcttccact ttgtgataga acgccagcac ttcttccgcc gattcacaaa gcgttacccg
     4081 atcgctgacc ggcacccccc actttttaaa ttgcagtaaa cggccaagat gagtatccgg
     4141 cagctcgcca ccttccagaa caccaacgcc atagcagaaa aaagtgagcg gtcgcttcgc
     4201 tgtaatacgc ggatcaagct gacgcagtga accagctgcc gcattacgtg ggttagcaaa
     4261 aactttcccg cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg cctgcggcag
     4321 gaacacttca ccacgcactt ccagacgcgc cgggatattc tctccgtgca gcttcagcgg
     4381 aatggcgcga atagtacgca cattagacgt gatatcttcc ccggtggtgc catcgccacg
     4441 ggtcgcggca ctgactaaaa cgccattttc atacagaata ctgacggcaa ggccatccag
     4501 cttcagctca cagcaccagg tgactttctc gttgtttttc agacggtcct gcacacgttt
     4561 gttgaaagca agaaagcttt cttcatcaaa aacgttatcc agtgacagca ttggtacttc
     4621 atggcgtatc tggctgaaag ctgccagcgg cgcagcacct acacgttggg taggcgaatc
     4681 aggcgtaatc agttctggat gtttggtttc cagctcgcgc agttcgcgca tcagcctgtc
     4741 gtattcagcg tcgggaattt ccggcgcatc catcacatga taaagatatt catgatggca
     4801 aagcgtcgtt cgcagttctg tcagttgttg ttcgattgat tccatatcgc accatcaatg
     4861 ctaaaaaccc ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa gtgtatcagg
     4921 cgttggcgtc tttgacttcg cggatgatgt cctggtactc gcgcaatttc tgcggagtca
     4981 tcatacggcg ctgatcgtca agcacgacac cgcccacttc atcggcaata tgctgcgcag
     5041 attgcagcat cagcttgaag ttctgcagct cgtcaccgta agacggtacc tgcataaaga
     5101 tagtgacacc cggagtagtg aaatccttca tttcaggatc aaaggttccc ggtttcacca
     5161 tattcgccag gctgaataac gccgggccgc tgccatccgg gctaagatga cgatggtaaa
     5221 tattcatatc gccaaaaatg aagcccgctt gttgaatgct gttaagaagc agttcaccgt
     5281 ttagctcgct accgtgatgc gccgcgacgt tcatgataat caccgcttct ttgcgcttcg
     5341 gtttatccat aactggagcc ggttccgcta caggctcagg ctgtggtgcc gctacgggtt
     5401 ctgcaggctg gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc tgcggcgctg
     5461 gcgcgacctg tggcgaaact ggctgctgca acggctgttc aggctgcggc tgataggcag
     5521 gctgctgcac cggctgcgct ggacgcggag catgttgcgg cggtacctgc gcttcaggcg
     5581 gctgctggac cggttgacgc ggctgcgcag acgcataagg cggtaggtac tggtgttgcg
     5641 gcgacggacg agcagcctca tgctcctgag cgttagccgg ggcatgattc acgcggtgaa
     5701 cacgaacctc accaacgccc tcatcatctt cgacatcctc gtcataagaa tcgtcgtcac
     5761 gttttgactt cattcgtttt aatggccgat cgcggaacat agaagatcgt tctttacggc
     5821 tggtccagaa accatgtacc agtaaagcga ttatggcgat cgcgccaaca atgattaata
     5881 tcagacgcaa atcctgcatc attatattct ctgttgttct aacaccttgc caccacggca
     5941 aacatttact cactaagagt atttgccgat tacctcaagt gcaagtgcac tattaacttt
     6001 cacagcacaa agatagatga aatcgtgctt tttgctgttt tttcgaacat atcctaactg
     6061 tccattgcgc aattacccgg taaaatacgc agaattttcc tgggattggt caaaaggagc
     6121 tcatcctgat tatggtttca tcattcacat ctgccccacg cagcggtttt tactattttg
     6181 cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt ttaccgctgc
     6241 tggtcaatat tttgttgatg gggggcgcat tctggtggct ctttacacag ctcgatgtct
     6301 ggatcccgac tttcatgagt tacgttccgg actggctaca atggctgagt tatctgttgt
     6361 ggcctctggc tgtcatctct gtgctgttag tgtttggcta tttcttctcc acgattgcta
     6421 actggattgc cgctccgttt aacggtttat tggctgaaca actggaagca cgattgactg
     6481 gcgctacacc gccagatacc gggattttcg gtatcatgaa agatgtgccg cgaatcatga
     6541 aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta ctaattcttt
     6601 acttcatccc tggtattggg caaaccgtcg cgccggtact gtggttcctg tttagcgcct
     6661 ggatgttagc catccagtat tgcgattacc ccttcgataa ccacaaagtg ccgtttaaag
     6721 agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt gctttaacca
     6781 gcctgtttac gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc gtttgtggcg
     6841 cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg taacaatcta
     6901 ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc ccttattcca
     6961 tcttgcatgt cattatttcc cttctgtata tagatatgct aaatccttac ttccgcatat
     7021 tctctgagcg ggtatgctac ctgttgtatc ccaatttcat acagttaagg acaggccatg
     7081 agtaagattt ttgaagataa ctcgctgact atcggtcaca cgccgctggt tcgcctgaat
     7141 cgcatcggta acggacgcat tctggcgaag gtggaatctc gtaaccccag cttcagcgtt
     7201 aagtgccgta tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt gctgaaacca
     7261 ggcgttgaac tggttgaacc gaccagcggt aataccggga ttgcactggc ctatgtagct
     7321 gccgctcgcg gttacaaact caccctgacc atgccagaaa ccatgagtat tgaacgccgc
     7381 aagctgctga aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa aggcatgaaa
     7441 ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc cagagaaata cctgctgctg
     7501 caacaattca gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg tccggaaatc
     7561 tgggaagata ccgacggtca ggttgatgta tttattgctg gcgttgggac tggcggtacg
     7621 ctgactggcg tcagccgcta cattaaaggc accaaaggca agaccgatct tatctctgtc
     7681 gccgttgagc caaccgattc tccagttatc gcccaggtgc tggcaggtga agagattaaa
     7741 cctggcccgc ataaaattca gggtattggc gctggtttta tcccggctaa cctcgatctc
     7801 aagctggtcg ataaagtcat tggcatcacc aatgaagaag ccatttctac cgcccgtcgt
     7861 ctaatggaag aagaaggtat tcttgcaggt atctcttctg gagcagctgt tgccgcagcg
     7921 ttgaaactac aagaagatga aagctttacc aacaagaata ttgtggttat tctaccatca
     7981 tcgggtgagc gttatttaag caccgcattg tttgccgatc tcttcactga gaaagaattg
     8041 caacagtaat gccagcttgt taaaaatgcg taaaaaagca cctttttagg tgcttttttg
     8101 tggcctgctt caaactttcg cccctcctgg cattgattca gcctgtcgga actggtattt
     8161 aaccagacta attattttga tgcgcgaaat taatcgttac aggaaaagcc aaagctgaat
     8221 cgattttatg atttggttca attcttcctt tagcggcata atgcttaatg acgtacgaaa
     8281 cgtcagcggt caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc gtcgcgtctg
     8341 ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga ctttagttcc
     8401 acaacactaa acctataagt tggggaaata caatgttcca gcaagaagtt accattaccg
     8461 ctccgaacgg tctgcacacc cgccctgctg cccagtttgt aaaagaagct aagggcttca
     8521 cttctgaaat tactgtgact tccaacggca aaagcgccag cgcgaaaagc ctgtttaaac
     8581 tgcagactct gggcctgact caaggtaccg ttgtgactat ctccgcagaa ggcgaagacg
     8641 agcagaaagc ggttgaacat ctggttaaac tgatggcgga actcgagtaa tttcccgggt
     8701 tcttttaaaa atcagtcaca agtaaggtag ggttatgatt tcaggcattt tagcatcccc
     8761 gggtatcgct ttcggtaaag ctctgcttct gaaagaagac gaaattgtca ttgaccggaa
     8821 aaaaatttct gccgaccagg ttgatcagga agttgaacgt tttctgagcg gtcgtgccaa
     8881 ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg gtgaagaaaa
     8941 agaagccatc tttgaagggc atattatgct gctcgaagat gaggagctgg agcaggaaat
     9001 catagccctg attaaagata aacacatgac agctgacgca gctgctcatg aagttatcga
     9061 aggtcaggct tctgccctgg aagagctgga tgatgaatac ctgaaagaac gtgcggctga
     9121 cgtacgtgat atcggtaagc gcctgctgcg caacatcctg ggcctgaaga ttatcgacct
     9181 gagcgccatt caggatgaag tcattctggt tgccgctgac ctgacgccgt ccgaaaccgc
     9241 acagctgaac ctgaagaagg tgctgggttt catcaccgac gcgggtggcc gtacttccca
     9301 cacctctatc atggcgcgtt ctctggaact gcctgctatc gtgggtaccg gtagcgtcac
     9361 ctctcaggtg aaaaatgacg actatctgat tctggatgcc gtaaataatc aggtttacgt
     9421 caatccaacc aacgaagtta ttgataaaat gcgcgctgtt caggagcaag tggcttctga
     9481 aaaagcagag cttgctaaac tgaaagatct gccagctatt acgctggacg gtcaccaggt
     9541 agaagtatgc gctaacattg gtacggttcg tgacgttgaa ggtgcagagc gtaacggcgc
     9601 tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg cgctgcccac
     9661 tgaagaagaa cagtttgctg cttacaaagc agtggctgaa gcgtgtggct cgcaggcggt
     9721 tatcgttcgt accatggaca tcggcggcga caaagagctg ccatacatga acttcccgaa
     9781 agaagagaac ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc gtaaagagat
     9841 cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat tgcgcattat
     9901 gttcccgatg atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga tcgaaatcta
     9961 caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa tcggcgtaat
    10021 ggtggaaaca ccggctgccg caacaattgc acgtcattta gccaaagaag ttgatttctt
    10081 tagtatcggc accaatgatt taacgcagta cactctggca gttgaccgtg gtaatgatat
    10141 gatttcacac ctttaccagc caatgtcacc gtccgtgctg aacttgatca agcaagttat
    10201 tgatgcttct catgctgaag gcaaatggac tggcatgtgt ggtgagcttg ctggcgatga
    10261 acgtgctaca cttctgttgc tggggatggg tctggacgaa ttctctatga gcgccatttc
    10321 tatcccgcgc attaagaaga ttatccgtaa cacgaacttc gaagatgcga aggtgttagc
    10381 agagcaggct cttgctcaac cgacaacgga cgagttaatg acgctggtta acaagttcat
    10441 tgaagaaaaa acaatctgct aatccacgag atgcggccca atttactgct taggagaaga
    10501 tcatgggttt gttcgataaa ctgaaatctc tggtttccga cgacaaaaag gataccggaa
    10561 ctattgagat cattgctccg ctctctggcg agatcgtcaa tatcgaagac gtgccggatg
    10621 tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat caaaccaacg ggtaacaaaa
    10681 tggtcgcgcc tgtagacggt accattggta aaatctttga aaccaatcac gcgttctcta
    10741 tcgaatctga tagcggcgtt gaactgttcg tccacttcgg tatcgacacc gttgaactga
    10801 aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt ggcgatactg
    10861 tcattgaatt tgatctgccg ctgctggaag agaaagccaa gtctaccctg actccggttg
    10921 ttatctccaa catggacgaa atcaaagaac tgatcaaact gtccggtagc gtaaccgtgg
    10981 gtgaaacccc ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa atggcgccca
    11041 tcggcgccat tttttatgct tccgccagcg gcggcaaaat caattcatcg ctctcatgct
    11101 gctgggtgta gcgcatcact tccagtacgc gcaaccccgc acggtgcacc gcatcgttta
    11161 acgccttccc tttcagcaaa ccactgataa gctgagcaca aaacaggtcg ccagtccctt
    11221 tcaggtcggt ttttacccgt gaatgggaaa tgacattcac gctgtcggca ctgaccacca
    11281 ccacctgcat ctcctgattt tcttcattac cggaggcgct ggtaatcacc acccatttta
    11341 atgtgtctga aagcagactt tttgcggcag caatggcact gtcgagatcg cggcaatttt
    11401 taccggtcag gatttccagc tcaaagatat tgggggtaat tccctgcgcc agcggcagta
    11461 aatattgtcg atacgcctcg ggaaggtcag gtttgacata aattccgcta tcaatatcgc
    11521 caatcaccgg atcgaccatg atcaataggt caggatggtc tttgcgtagc gcagtcagcc
    11581 actcggcaag gattttgatt tgcgatgccg ttcccatata gcccgtggtt acagcacgaa
    11641 gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac cattcgtccg
    11701 gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt gctcagcaat accgtcggca
    11761 cggcaaagac attcaagccg ttctgtttta tagcaggcac cgcaatgctg ttgcctacgc
    11821 ttccgtacac tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc ctactcttat
    11881 cgttaaacaa caacaaacta ctcatttaat tttttctcct tgccgatgat cctcatcgta
    11941 ttccaaccga aactttacct gattctggca gtcaaatcgg ctattacaaa acaaagataa
    12001 ggtaattcag tgaagaaaat catttgtctg gccattacgc tgctaatgac actccccgct
    12061 tacgcgaagt taactgttca tgaagaagcc cgcatcaacg ccatgctgga gggattagca
    12121 cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac atacctgcga tgaagcggtt
    12181 tcccacctgc gtctgaagct cggcaatacc cgtaaccgca ttgacactgc cgagcagttt
    12241 attgataagg tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt gaagatcccc
    12301 ggtaagagcg atgagaacgc acagcctttt ttacatgcgt taattgcgca gacggataaa
    12361 acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc atttacagga ggtaacatga
    12421 aaaaatgctt tatttatcac gatgaaaaat cgaataaatt ttggtggata gattacgaag
    12481 gggatagttt agctgtcaac tatggcaagg taggtagtat tggtaaattc catacaaaag
    12541 aattcgataa tgaagaacag tgtctgaaag aagccagtaa attgattgcc gcaaaaatga
    12601 aaaaaggata tcaagaaact ccagacttta acttcatgga tctctactat tttgatgatg
    12661 aagagattgg gttacatctt aaaacgtcac acccaaactt ccagtgccat tttactgatc
    12721 cactttatat gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa ggtgctgatg
    12781 ctctaaacga acttgaaaat agcctccgtt ctggactgtg ctgatttccc tcaaatgtta
    12841 attgaaacta tgtggggtat gaaatacatc gctatggaca gtattcttga agaggatgtg
    12901 cgtgcgcaat tactagccga tgaaatgagc tctatccaga gcaatatgat tacctacgcg
    12961 actgcattcg gtcagatcaa agtcatgggt aaaatctccc ataaacttaa aaagatggga
    13021 ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg tgacggtcag
    13081 gactcaccaa tacttcaaaa aatgattgat gaccttactg cttttccaca cgaaaattaa
    13141 aaactgcatt tgtcggcagc aacaactgcc gacaaatcag attattgaca aagtgcactt
    13201 tgtttatgcc ggatgcggcg taaacgcctg atccggcata aaaatattgc taattcaata
    13261 tattgcagaa acattgtagg cctgataagc ggagcgcatc aggcagtttt gcgtttgtca
    13321 tcagtctcca atgctattaa tccttaaatc cctgccccct gactaaaatg ctcttcccca
    13381 aacactccgg tggagaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
    13441 ccagggttag cttttgccac ccgcagtgct ccagcaaccg cgccgcctga gctgacgcca
    13501 cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc gcgctgatga
    13561 atatccagca cctcatccac cagcgcagcg ttgaaaatcc ccggcagata ttccgcaggc
    13621 cagcggcgaa tgcccggaat actgctgcct tcttccggtt gcaggccaac aatggtcacc
    13681 ggtttggatt gttcgcgcat aaagcatgaa acgccggtga tagtgccggt cgtccccatg
    13741 ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg cccggtggtg
    13801 gtgtaatgcg cataagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca
    13861 cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca
    13921 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
    13981 ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg
    14041 gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc
    14101 gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct
    14161 tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga
    14221 ggcgtattgc ctattgtttg ttctaatgta ctcacaatct ctatccatac gtggtgttgc
    14281 ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc cattaaaaag
    14341 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
    14401 tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat acagccgcgc
    14461 atgttgcaga ccaacgaata aacgctcgcc atgctgcggg gcatcgtcgc catgcatcac
    14521 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
    14581 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct
    14641 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgatacg caggtgtgta
    14701 gcccagcggc cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
    14761 gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc gccatacctg
    14821 attcggcgca tcagcctgtt caatattccc ctgactcatc accactacac gatcggctac
    14881 ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg gtgaatttca gctcttcatg
    14941 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccgaacgg
    15001 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacacgctg
    15061 tttctggccg ccggaaagct gcgccggata acgatccgcc agatgcgcaa gctggaccat
    15121 ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct cgcgacgcgg
    15181 cagcaccgtc aggccaaaag tgatattgtc gaataccgtc atatggcgga acagcgcgta
    15241 atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca cgtcggtgcc
    15301 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatcc gcagcagcgt
    15361 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag gaatatccag
    15421 tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttgatattgg caatctcaat
    15481 gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
    15541 tactctttaa aaacagggtg ataatcgcca tcaacgtcaa cagcgcggcg gcggtaaagg
    15601 agccgacggt gttgtagtcc tgctccaaca attcaatctg taacggcagc gaaagtgttt
    15661 cgccgcgaat cgagccggaa accaccgaca ctgcaccaaa ctcgccaatc gcgcgggcgt
    15721 tggtcagcac cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg acgcgacgga
    15781 acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
    15841 ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca
    15901 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccga
    15961 gcgggccgtt ggagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacag
    16021 caaacggaat gtccagtagc gtcagcagta actggcgtcc cgggaagtta aagcgcgtca
    16081 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
    16141 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
    16201 tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc aggatgaacg
    16261 ccgaaaccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
    16321 cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg
    16381 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
    16441 aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt
    16501 cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag
    16561 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagct ccggcagcac
    16621 cactttgcag aaactctgcc agcgcgttgc accaagcgtt tccgccgctt cttcatattc
    16681 cgggcctaac tcttccagca ccggctgcac ggtacgcacc acaaacggaa tgctggtaaa
    16741 ggccatcgcc accgcaatac caagccaggt ataggtgact ttgatatcaa acttcgccag
    16801 ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac cggcgactgc
    16861 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
    16921 ataacgtgtt aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac
    16981 aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg tgatcacctc
    17041 ccagtactgc gcccagctca tctcagaaag ctgcatcacc agcgcagaga gcggcagcag
    17101 caatatcagg cacacaaaca gcagactggt gccaaggctt aaagtaaagc ccggcagcac
    17161 gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt gtctaactcg
    17221 ccgccgctgg tgaagtgggt tttcatcact tccggccatg agccaaattt gtcttccacg
    17281 cggaacagtt cggtctgcgg gaatttatct ttcagtttgt ccatgacttc cgggttattc
    17341 acacggtagt aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag ccagttcagg
    17401 taggcttttg cggctttttc cgtaccgttg gcctgcacat ttttatcgac ccacgccacc
    17461 gggaattccg ccagaatatt ggttttcggg atcaccactt caaagccctg tgcttcatac
    17521 tgtttacgga tgttgttcac ttccgattca aagctaatca gcacatcgcc caagccacgc
    17581 tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac gtttttcagg
    17641 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg
    17701 ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
    17761 agcttcacgt cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
    17821 cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag gcgcgactgc
    17881 cagtcagccg ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt cacctggtta
    17941 taagtgacaa cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt tgatgacccg
    18001 gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca ttgctgttca
    18061 aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
    18121 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
    18181 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
    18241 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
    18301 taaaaaggga ataagaactt ggttcaacag gcgtaattgt tgcaggcagt ttggacacgg
    18361 acagcgcgga gaaaccggag cgtacacata gtacgtgagg atttcgagca ctgcccgggg
    18421 ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc tccccacaca
    18481 tgcagggagg aaacagaggt gaatcagata ccgacgctaa ccgtctccgg cagtgtgctg
    18541 ccgccatcaa tcacattctg tgtaccggtt aaatagctgg attcatccga tgcgaggaag
    18601 gccgccagtt cgccgacttc cagcggatcg gcgaggcgac acagcgggat tgcttttgcc
    18661 atttcagtca gcaccgattc tggatcttcc gggttcgact ggcgggcaat gctttccgcc
    18721 attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc gaataccgga ttgcgcgtac
    18781 tccaccgcca gcgatttagt caggccaaca atcgccgctt tcgttaaggc atacgccgtt
    18841 tcgccaggat cggccaccat atcaccagtg actgaagaca tcatcacaat gcgaccatct
    18901 ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc tttaatattg
    18961 atatcaatat ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc cagacgacaa
    19021 acgcctgcgt tattcaccag gatatcaatg cgcccttctt tttccttcgc gcgtttgata
    19081 gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca gcgatgacca
    19141 cgaccacaca gttcgtccgc tagcttttcg atctcagggg agatatccag caagattagg
    19201 ttcgcgccat gacgtgcaaa agttctggca attccttcgc caattccctg caatgcgccc
    19261 gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa tgctctcctt gttaatacag
    19321 agcgttacac tccattccct ctgaaaatac aggataaaca aaatattgct gcggatcagc
    19381 tcactaattc aggaaaatct caatccagtc gctgctttat gcatcatttc aggcgttgtg
    19441 ttagctcact gctgcgctga atcattttca atgacgccac atcattgatg ttgcacaaga
    19501 ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc gggtagacat cgacgagcta
    19561 cgccattcgg tttcaccgga aacggtatcg agcgtgaaat gcgccagccg ccgcagcgga
    19621 gagtcagcca gagaagtaat ggcaatcacc gttgccccct gctttcgtgc cgcttctgcg
    19681 cacaagacaa tctctttttt actgccgctg taagagatag cgatttgtac atcgcctttt
    19741 ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc cacgcgatag
    19801 ccaattttca tcagtttgaa cgacagatcg cgccccacca gcgccgatcc gcccaggccc
    19861 gttatctgga taaacggtgc tttactgatg acctcaatga ttttttgcag ccgcgcgtag
    19921 tcgaacaacg cacaggtttg ctccagcgcc agctcttttt cgcgattcag tttgcgggca
    19981 atcacttcca gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc cgtggcattc
    20041 gttttctccc ggctggcact gtattcgcca attaacgcca tacgcaattc agtaaatcct
    20101 tgcgcgccga gcttctgggc aaatttcaca atgctcgact ggctgatacc gagctgtttc
    20161 gccatctgcc gtgaagagac ggatttcagc tcgctcactc tggcccgcag aaagtcagca
    20221 attttttgct cgttcccggt aaattcactt tccgcattgc gtattttcgt caggtacaac
    20281 atcttccact ctcctgggct ggcagcaaaa attccgctta agttcttatg acgctcttca
    20341 cactctgcga gtgtagactc aatgattcct ttaagtctgt caattcggga tatatgattc
    20401 catacatctg caattatttg atcgtaaata gtaaggtcac caccgatgca actagaaaag
    20461 atgattactg aaggctcgaa caccgcctcg gctgaaattg accgcgtatc gacgctggaa
    20521 atgtgccgga ttatcaacga tgaagataaa accgtaccgc ctgccgttga tcgcgtcctg
    20581 ccggatatcg ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg cggtcgtctg
    20641 atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag cgaatgtccg
    20701 cccacctacg gcgtgaaacc gggtctggtg gttggtttga ttgctggagg cgaatatgcc
    20761 attcagcacg cggtggaagg agctgaagat agccgggaag gtggtgttaa cgatctgaaa
    20821 aatattaatt taacggcaca ggatgtggtg gttggcattg ctgccagcgg tcgcacgccg
    20881 tatgtgattg ccggactgga atacgcacgc cagctcggct gccgcacagt gggaatttcc
    20941 tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg ccattacacc gattgtaggt
    21001 gccgaagttg ttaccggttc ttcgcggatg aaagcaggta cagcgcagaa actggtgctc
    21061 aatatgcttt ccaccgggct gatgattaaa tccggcaaag tgttcggcaa cctgatggtc
    21121 gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt taaaaacgcc
    21181 accggatgta atgccgaaca agcggaagcg gcgttaattg cctgcgagcg caactgtaaa
    21241 acggccattg tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa acgcctggat
    21301 caacacggcg gctttattcg tcaggtttta gacaaggaat aacccatggc caaagagatc
    21361 agcagtgaac ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa tatcgccagt
    21421 tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcact ggttgatccc
    21481 aatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgacca ggtgcaggtc
    21541 gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct gctgggcgat
    21601 gcaccggtac aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt aaaagccaaa
    21661 caaacctccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac gccgctgatc
    21721 cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc cacggtgatg
    21781 cacgttccgg cagatgctca gggcacgcta cccgacgcgc tgaattttat gaaggtgttc
    21841 agcaaaggtt tgttcacctt cctagtgatt ctggtgggct ataacgccgc ccaggcattt
    21901 ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg ttataacccc
    21961 gctgcaacca ccggttacta cgccggtttt cacgatttct ttggtctgcc catcgatccg
    22021 cgcggcaata ttatcggcgt gctgattgcc gcgtgggcct gcgcccgcat tgaaggcatg
    22081 gtgcgccgct ttatgccgga cgatctcgac atgctgctga cctcgttaat caccctgctg
    22141 atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gtggctggtt attcgaaggt
    22201 atgtcatggc tgtttatgca cctgaacagt aatccgttcg gttgtgcggt tttagccggg
    22261 ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt ttacctcgcg
    22321 ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc aggcgcgggc
    22381 caggtgggcg cggcgctggc actctactgg cgggcgcaac cgcacagtgc gctacgcagt
    22441 caggtacgcg gggcgattat tcccggcctg ctgggcgttg gtgaaccgct gatttacggt
    22501 gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc ggcgggcggt
    22561 ttgtttatcg gattgatagc ctggtggggt ctgccgatgg gcttaaacag cgcctttggg
    22621 ccgtctggtc tggtggcgct gccgctgatg acttccgcac aaggcatcct tccggcaatg
    22681 gcggtttatg ctggcgggat tctggtggca tgggtttgcg ggtttatttt caccacgctc
    22741 tttggctgcc gtaacgtcaa tctggactga tattatgaaa cggacaatgc tctatctttc
    22801 tctgctggct gttagttgta gcgtcagcgc cgcaaaatac cctgttctga cagaaggtag
    22861 ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg ccagtcagta atgttactgc
    22921 ctggccttta atcaatgaat cagaaacgct tacagcgcca tcaacttgtc cagcgacggt
    22981 gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc acgtttgcca
    23041 tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg cgcgcagtag
    23101 gcgcagaagt acagaccgtg agtgccactg gcggtgccgt acggcaggct ctggcgaaca
    23161 atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga ggtttccgga
    23221 cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat catctcctga
    23281 tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct cccaacgctg cacaaacaca
    23341 tagctgccgc cggaatccac gccgtctttg ataaccgcca cttcgcggcg cgtctcttca
    23401 cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc cacccaacgg
    23461 aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat cgccgcctga
    23521 gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac atcgaactgg
    23581 gtcgtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc tgccccaacg
    23641 ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc aagatgcgcg
    23701 tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact ggccgcacgc
    23761 agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg gcaatgttct
    23821 ggcaaaatgc cactctgaac ctgagacatt gttcctcctg aaaataagaa tgccacgcaa
    23881 tccgtggcat tgttgagcgc tattttacgt gttttttcgc gacggcggtt tgttcagacg
    23941 caaattaacg ccgccagata attttactga ctttccagtt tttcagggta tcgtccgaag
    24001 gcattaaccc ttcaggacca cgccattccc cgctgaactg gtagctaata tgttgactgc
    24061 cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg caattgccaa
    24121 atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg tcagcaggaa
    24181 tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta atcaccatcg
    24241 ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac gttgccgttc gcggttttca
    24301 ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc aatggcttgt tcttgcagtg
    24361 gtgtggacgc cgttaattca cccacgccct gctcgctcac tttggtggac gatccaaacc
    24421 agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag ccggtcagca
    24481 tcaacggcat agcgcataac attaaacgca gcgatttcat gtcacttcct ttggttattc
    24541 aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt tcttcattaa
    24601 aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata tccgccacta
    24661 ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac ggttgccagc
    24721 aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag cacatgccag gtgcgaggaa
    24781 acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg ggaatcagcc
    24841 ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta ccctgctcac
    24901 gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat agcacaagcc
    24961 agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga tggaactcag
    25021 tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg ttcatatccc
    25081 aggcgttcat acattccgag caccatgtcg ttatcttccg gcacgttgat ctgaattttc
    25141 gggcagccgc gagcaatcag ctttttctcc agccggttaa gcaacgcatt ggcaattcca
    25201 cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg cccgtcataa
    25261 ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa caaactgacg
    25321 tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg cagcaagtcg
    25381 caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac gcgtatctcc
    25441 atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac ggtcgaatta
    25501 gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt caactattga
    25561 aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa cggattacgc gatacgatat
    25621 aacatctgga actttattat tacaactcag gccgtatgag cacttttaaa ccactaaaaa
    25681 cactcacttc gcgccgccag gtgctgaaag ccggtttagc tgccctgacg ttgtcaggaa
    25741 tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag caacggacac agcaagccga
    25801 aagccaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac ggcggaattg
    25861 ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg gcgattgcta
    25921 aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg cgttctggcg
    25981 atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc gcagatctgt
    26041 ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct tcggtatttg
    26101 ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa cgcgaaaacc
    26161 gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg cagcaagtac
    26221 tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgcttggc tcgcatattc
    26281 tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa gccgcgtttg
    26341 tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt atcaccaacc
    26401 cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc acagcgattg
    26461 ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat tcgaaaaagc
    26521 gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac cttcaggata
    26581 cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat agttggcagc
    26641 gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt ttcgaacagg
    26701 cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc tgcttccgcc accgctcatc
    26761 gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta gtgcatccgc
    26821 ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc gaaaaaccgg
    26881 gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt aacccctttc tatggttttg
    26941 aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgccagcca tttggtgaag
    27001 acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa catcgcaacg
    27061 aacagcgcgg cattggcggg ctgttctttg atgatctgaa cacgccagat ttcgaccact
    27121 gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta ccaattgtag
    27181 agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc taccgtcgcg
    27241 gtcgttatgt cgagttcaat ctggtctggg atcgcggcac gctgtttggc ctgcaaactg
    27301 gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg gagtatgatt
    27361 atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag gtcagggatt
    27421 gggtgtaact ccctcacccc cattcccgca tccgctgatg caacgtcaac gactgcttct
    27481 cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat ccccactgca
    27541 tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg cggcgtacgg
    27601 cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata gcgtgaaacg
    27661 cgttttgtag cgtgcggcga ctgacatgca gttgattaca caaatccagc accgtcaccg
    27721 gttcggacat gttttccagc acatattcac gggcgcggga aagcaaccga cggtaactct
    27781 gatgactgat gctttccgcc gtcaccattg gttgcgcttc ttccagcatc gcccccatcg
    27841 ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga ttttccgggt
    27901 tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct ttatgctgct
    27961 ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc ggattgtgca
    28021 aaaagttagc ctgacaggtg atgacatctt cagacagcac tacgcccagg atcgtataat
    28081 cgtccggcgt acttaattca aactcggttc caccggggcg cgtcgctatt tctgcgcttc
    28141 ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg ccaaaccaga
    28201 acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat tcacgaaaaa
    28261 cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc ggatgcagct
    28321 gatcgtaaat ctgctgctag gcggtaatcg tcaaagcatg ttcatagaca tccgttgtct
    28381 gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt tcgggtaagg
    28441 gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg ttaatgccgg
    28501 gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga taacgactac
    28561 tgcgtttacg taacgtcccg gcagaaaaga gctgttcgag cgcatttctg gctttttcca
    28621 gcggccagcc aaagtgggcg gccacttctc ctgccgtcat tccctgacat actgatgtta
    28681 acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg ggttccttta
    28741 cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca tcgtctggcc
    28801 gaccgatctc cttacggctg atgacacgcc cggtacgcat cgcggcagca caacctgcgt
    28861 ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag cgaccagggt
    28921 caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca tccgctgcat
    28981 ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc atcggcaacc
    29041 tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg gtaaaggcgt
    29101 tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag tagttggttt
    29161 cagacggtgg cgggacgtag gtcaccagct gtacgtcggc agatttcaac gcggcatcaa
    29221 tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg ccaagcgtga
    29281 tcccggcggt tgatgaaaga taagaaccgg tacgcgaaac cacatgcgcc aggaaggcgg
    29341 tgttttctgc gtcattggcc cactggaaag ctgcgccatt ttcaatatgt gcaaccatcg
    29401 catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc agcacttcac
    29461 cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag cgagcgacca tataccactt
    29521 cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg tcagaatcgg
    29581 cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc gcaaattcag
    29641 cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa tctaaagctg
    29701 gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc agcatacgtt
    29761 tcgccaaatc cacaatgacg gcacagcagc acgaaagaga agcaaaagtc agctacctgg
    29821 tacactaggc agcgggtcag caaagggggg caaatgcccc ttttttcatg tcagataatc
    29881 agatcattac ctcaataaac taataatcac atcgcggtta caaccagttt gatagtgata
    29941 gctcaaaccc agacgtggcg ctggctgcaa gcaaaataag ccgcaaaaat gcgaccccat
    30001 gtttactagc cgcaaaaatg cggtcctatt tgccagaacc ttttattatt caattggtta
    30061 gtgcaagaaa aagcgtgatt actgttcaag aagtatccag aaaggtcatg attttgacgc
    30121 tgaaaaaaaa taggtgggca tttttgccca ctcactagcc gcaaatttgc ccactcacta
    30181 gccgcatttt tgcggcccaa agtaataaat acagagaaat aataaataca gatcagcgcc
    30241 aagatttttc gtcttttcgg cggaatcatg agactagtgc ttacgtcctg ataacctcca
    30301 gagcagacct taacttctgt gcagcaggac tacgctcatt gccgctcggc taggtcaaat
    30361 cctcacgata aagctgttcg gattgtgacc ataatgttaa aggactcttt aaatcattcc
    30421 ttgacgaatt aactagctgg cttaagcgga atagcatgca ggataaaaac aaaaccagct
    30481 tagcagtggg gagagcttcg cttcgctcgt ctcctggggc caatgctcaa tgaaacgacg
    30541 taaggcacgg agcgctcacc ataggtagac acataaacat ctgagagcgc accttctatc
    30601 gcgctacgcg ctcaataaaa accaacgaca ccgccacact gcaagaccaa tggtttttat
    30661 cgcgtataca gaccagacag gaaacagcag caaagcagcg tatagccagg tgcagacagc
    30721 acactcacac gacgcacagc aggcttaagt gatcactcag gaaactttat ccccgccgcc
    30781 gatcaccgcg aactatcatg accgcgccaa attcgaaagg aatggcccat agaacgcgat
    30841 gaacacacat aagcgctaca cttacttaac gtcgccacag agcgattgag agcgcttaaa
    30901 atcgatgcga tttcattaaa tttttctcac tcgcctgaat ggtggcgatc aggactgatc
    30961 gcaacattgc cccgcctcga tattgagcgc ataatagata catacattaa gcggaaaaga
    31021 gataagttta ttgtgaaaat cagggggtta catatcatgc gcatatcaga acaacaacgg
    31081 cagatcctcg actatctggc aaaaaacgaa tcaatcaccg cacgacaagc cgcagatctc
    31141 gtttatggtg gcaatgttac ccgcgtccag gttgacgccg cccgccgctc actcatgacg
    31201 atggttagta atgggctaat caggaaacaa ggccgcgctt tcgtagctga aagacatatc
    31261 aatcacgctt atagccagga cgttgaagaa ttgcgccgcc agatcctttg ggtattcagc
    31321 aaacagggtc aggagtattt cctttctgaa cacccaaatt tcgccgccaa agagttaaca
    31381 gtaagcggat tgtcgtgcgc actggattat cttgtcggtg aaggttgcgt tacttacaag
    31441 cagctatggc acgccctgaa tacgcttgta gaagatggtt tactcattgc cacgcgtgag
    31501 aacatcgaat actggataat gccgcgttca ggtcgccgca atcatgcacg gtatatttca
    31561 ggcaacagat acagaacgcc ggactttaat ccgacacagg aagatcggga ccgtgccgaa
    31621 atgatgtgca gggaacagga gaaaagaatt atggataagt tattttcgat gcagtgaaca
    31681 caagcgggcg caatgcccgt ttttattgcc ttattccacg cctgacgaat caatttgatc
    31741 acacctgaga aaatgcatag ccagaagtta aggccgttat attgcgattg ggagcgtttt
    31801 aaacggtatc aatttaatta attctgatgt ggtgatttgc aggcaaaaaa aacccgccgg
    31861 agaagcggga aatagagaca tatcgttgta gttattaaca gatcactttg aggtcgaggg
    31921 cggctaaccc tcgctaatat tatcccgcta ttaccttgcg ggtgcaaata atcttttatt
    31981 gctttgatga tttaataaat agcacttttt gctaacaaaa actgctgtca atatatggcc
    32041 aaaaatgtca acagaaaaaa taaggtttat aatattaagt atcacatggg taatgggttc
    32101 agggaatcgg aatccaccgc ctcacggaaa ttcaaaattt gcttcttctt tcctccgtgg
    32161 gcggcttctt tttcgtatcg aggtatttat gaaagcaata acattagaac agttcgcagc
    32221 aatggcgaat gtatcgacca gaacgctgag gcgctggcta aaaaaaccat caaccggagt
    32281 ccctttgcca tttaccccac gcggtactcg ccaattattc tgggagcacg aatgcaaaga
    32341 atggttgtac agaacagaac gataataatc acgcatcaca ttttgaggta aacatcatga
    32401 ccaatatcga aagattagaa cgctgtgccg atattatgcg gcgcagatgg atttatgacc
    32461 ctgaagcggg tttattgttt agccgtgaaa caaaagacgt tatcaaaggc agcatgacca
    32521 ataaagggca tttggttgta acggtacatg aaggtaaatt caaagtgaca ctcacctatc
    32581 agaaagcctg ctatgtatac acatacggcg cttatgacga gacattatat gaagtgcacc
    32641 acgttaattt gaacaaacag gataaccgca tcaagaatat tcgcctgatg ccgaaagaaa
    32701 agcaccgccg gatacataaa aacgtccgca aacttattcg gctgggccac acacaatggc
    32761 ttcaaccgca attaattgaa caatcactat aaacacagga gtaaatacca tgcattcatt
    32821 agacagttat tttcagcgca cgacagcccc taaatcagcc gcccaggaac gccgtgagga
    32881 attccatgag aaagtgatgc gttctgctga ttatattgcg gacaagttcg tggaaacggt
    32941 tcgtccactt gttgatgaag tagctgataa acttcagtct gaaatgccgg aagatatgga
    33001 aggcactgca aagcggcgac tcatttgcga attaagtcgt cgttttggcg tgtcaataag
    33061 cgcttttaaa taatcgaaaa aaggggggaa agataatgga cgcatttgag gtactggcaa
    33121 ataaaggcgc tgaactggta gcacagcgtg ataaaaccgc caacgaaggg gaacgcgcac
    33181 tgctaaataa acagattaaa gcgatccgta tggcccagtt taaattaatc agtaatgaag
    33241 ttatcgaaac aattaaaccg cagatcccac aaatcgttgc tgacgctatt aaagccgccg
    33301 gactggagaa acgcattgcc gggctaaaaa cgaacgcaga gaaaatggag atgcacaaac
    33361 agagcggtgc tccgaatccc gatgagtact tctcaccgga tgttgagttt atggcgcagg
    33421 ttgaggaacg tctgggtagc tgtctcaccg aggaacagcg tcgctatttt gatggagtgg
    33481 atagttcggc aggtattgac ctgaactcgt atttcggtcg tgaaattgag cattttgatg
    33541 ccgccccgtc tatgccggag ccagaaccag aaatggccac cgatcctgaa gctgagcaac
    33601 ggaaacgcgt attcaatgcg atagggattc ggtactgatc ggataaaaga aagccgggaa
    33661 ttaccccggc tgacttaaca atgtgaccaa tgaacaaatc ggacaagaca aacaggataa
    33721 gcagtctcga aacaatgttc gggaactata ttagcacaag tccggagaaa atcatcatgt
    33781 tggcactacg catcatgcag ggcatcgcta agaccctcgc agaacatgtc ttagacctga
    33841 aacattcgcc actgagcaaa caggccatga agcgccaaac gcttcgtctg tgggctgaat
    33901 attcgcttgg aactatcaat aaaatcatcg acatgaaatc ggggccgagc aaccagagcg
    33961 cggaggaaat ggagttcatc aggcgtttaa ttctcattcg cagagacatt catagccagt
    34021 tacacagcgt gggaatcgat attaatgatg ggacgggcga ttaattttcg gacaagtact
    34081 atgcgcgcgc gaggagctga acatgaagca agatattgca gacaggctgg agatactcga
    34141 aggccagcgg gcagaagcga aacaactacg taagcaggcc agaagagcgc acaggaacaa
    34201 cgaagcagag ttactaacca aatacatcag tttcaccaat tactgcatct acgagtgtta
    34261 caaggaagat gcagaggact ggctggattc attgccagaa cagtactgag cagaataaca
    34321 ccacgtcatg ttgttctgag gtgagcatat cacctctgtt caggtggcca aatttagtgt
    34381 gccacttcag catcaccgtt gaaatcacaa taacgatttt tttgtaaata tgctctcaca
    34441 tatgtaggaa tagaccaggt tggcattgaa acacatctac catgaccata ataaagtaat
    34501 tttttgatat gtttatggtt ttatattaat taaaatatat ctaaaattga tagttttaaa
    34561 attgcaaaat ccagattgtg tttgtaatgt ttaatctgat tgtattgagt ggcgtttatt
    34621 atgaaaaata aatcaggtaa ttcttgttat agagaagcag tcgatattca tagctcactt
    34681 gagctttcat ttatattgca taaatatcat ttgcgtgtag atgagaagaa atctcgaaat
    34741 gtattcagcg ccatacgtga tgagctcatc cttgatgggg attcacgaca aaacttagct
    34801 actttctgtc aaacatggct tgatgatgaa atccgtgagt tgcttaattt agctattgat
    34861 aaaaacatga tttataaaga tgaatatcca caaatggcgg aacctgaagg tcgctgtgtg
    34921 cgtatgcttt ccgattcgtg gagtttcccc gattcgcgac acaccttagg ttgctcaacc
    34981 attggttcat ctgaagctgc tatgctcggc cgcttggcgt taaaatggca gtggtgtaaa
    35041 aaacgcgaag tacaaggtaa atcaaccgaa cctgatttgc gatcctgtgc aaatatgctg
    35101 gcataaattc tgccgttact gtgatgtgaa attgtgtgaa attccacttg atgatcatca
    35161 cctgataatg aatgctaaag aggttttgaa gcatgtcgat gaaaacacta ttggtgtcgt
    35221 tacgacttta ggtattactt atttttgtaa agcagattgc gttagtgttt ttaagataag
    35281 tgttaaagtt aatattagtt tttatattta catcgtaatt atatgtttct attttaaaaa
    35341 tgttcgagag tataaaaatg tctaatgcta atatcgcacc caaaagtaag aaaatgacag
    35401 tggggacact cgctatcatg aatataactg ctgtagtaag tctacgattt ataccttctg
    35461 aagcagaata cggcttaggt gccatttttt actatacctt tgctgcaatt atgtttttag
    35521 tacctgttgc ctttgttgcg gctgagctag ctacaacata tccacaaaaa gggggggcat
    35581 ttcgatggat aggagaagca tttggggctc gctggggttt tgtaggaatg cttatgacct
    35641 ggctacaagt catcccctac tttccaacag tactgacatt tggtgcagtc tcggttgcat
    35701 ttattgatgt tgatatgggg gtggcagaat caattgccag taataaatgg tatattttat
    35761 ttttcgtact atttgtttac tggggagcta tttgtgttgc attacgtggc gttggtattt
    35821 tctcgcaggt atctaagtgg tgcggtatta tagggacaat tattccagct attatccttg
    35881 ttattcttgg tttctcctat ctgtggttca gcggtaaacc agcggtgatc ccattaggtt
    35941 ggggtgatct tttccctaat atagctaact tccagaatat tgttcttgcc gccagcatat
    36001 ttcttgctta tgcagggatg gaaatgaatg cggtccatgt taacgattta gataatccaa
    36061 ctaaaaaata cccaattgca attaccatcg cttctatagg tactattgca atattcctgc
    36121 tcagtacttt aggggtcgcc tttattattc ctacagataa aattagtcta actcaaagtt
    36181 tgttggttgc ctacgatatg ctgttcgaat gggctggagt gccatggctt ggctctgtga
    36241 tggcatttat gctagcaatt ggcgtactgg gtggggtggt tacttggatt gcagggccta
    36301 atacaggtgt attagcgatc gcgaaagctg gttatttacc taagtttttc caaaaaacca
    36361 atcgtcatgg aatggggcac catctaatgt ttgtacaagg tataattgtc tcagttcttt
    36421 ctgttacttt tgtgataatg ccttcagttc aagcagcttt tcagatttta tcacagttaa
    36481 cagttatgct ctatttagtt atgtatatgc tcatgttcgc tagtgctatc tatctacgac
    36541 attctcaacc tgatacaaaa cgcccatatc gagcccctgc tttatttttc tgggcaacac
    36601 ttggattctt agggtctttt ctggcttttg tattaagttt tattccgccc gagcaaatcc
    36661 caactggtag tccaaccagc tatgttcttt atttgttagt attagttgcc ctatttttat
    36721 taattccaat attaatttac gccttccgaa aatctgactg gaaagatcca aactctgatt
    36781 tcgaaccatt ttcttgggaa aaaagaacta gtaattaaat tttccataag ctacaaaaga
    36841 tgaggttaaa tatgtttagt gctgaatctc caattggtgt aatagactca ggtgtaggtg
    36901 gattgacatc ggtaaaggaa ataattaatt tgcttcctgg tgaagatatt atctattgtg
    36961 gagataatat gaatgcacct tatggcaacc gttctgccga tgatatcatc agtttgacta
    37021 aaaaaatgct gactttttta cagtcaagga atgtcaaact agttgctgtc gcatgtaata
    37081 ctatatcctc aactttggaa tccgaagaat acagcggata tgctaagagc tttccgtttc
    37141 ctatcttatc catcattgaa cctgcagtag aagatgtgat tcgccagcaa tataaaaatg
    37201 ttggtattat cgctactgag tttactatta aaacggggtg tcacaaagag ctaataaaaa
    37261 aattgaattc aacaataaat gtatttggag aaccaagcaa aaatcttgca atgttaattg
    37321 aggaaggtaa tcttaatgct cctgcaatct tgaatgacat caaaaaacat gttaaccatt
    37381 taatttccct ccatccagta aatgaaatta ttctcggctg tactcactat ccaattgttc
    37441 agaatttttt tgaagcagtt gcaccggata taaaatttat taatcctgca catgatcagg
    37501 ctatctctat taaaaatcac ttgggacagc taaatttatt aaactcctca aatataggaa
    37561 ctttgcaaat aaatactagt ggaagtatgg agatctacaa aacggtacta ggggaattga
    37621 gcattacgaa acctcataca ttcagtatca ggcagtttta aatttatccg gtgcaccgta
    37681 aaatcccgtc cttctgaact gcaccccaaa agttggacac caactgagta aggtgcattt
    37741 tatggacaaa cccagttaca tactgggtag tgcatccaat tagtagaaca tgtgtttttc
    37801 gataaacgct ccgatcactt tttcgtggat ctcaactgag cgtgagaagc agattgtttt
    37861 acgacccaac cgcttaatgc gggtgcgtag cgtcagatta ttacgctcaa tgcgttgggt
    37921 gaatattttg ccggtcagat gcttattctt cggcacctcc cggccatagc tgccccagtc
    37981 atcgctggtc agcatgccga tgttgaaggg tgtaagcagt gccagtagct cccggcacgt
    38041 ttgatcggtt cggggaccaa aagtgtaggc cagtacaccg cctgttttgg tgttgtacgc
    38101 gtacccgagc caatgttgcc gggctttact gccaacgtag ctccattgct catcaagctc
    38161 gcagataaac gccacatcag catgggcaac aggcgaagaa gttattcgct ttggcgcgag
    38221 ttttttaaag tccggatgac ggtgttacta ccaattttca gtgtcctggc ggtatcgcga
    38281 accccggcac cattaaaggc catttcagtt atcagctctt tcatacccgg cttacgtgct
    38341 tgataagtat aagtgaactg aaacacacgg tggcagtcac ggcagcgaag tctgtcacgg
    38401 cctttagggt tctgaccatg gcggtaaacc tgagctgact gacaacgggg acaatgaata
    38461 ttaacgctgg tcatgagata acctcaaaag cccgtattat acatcagatt caactaatta
    38521 gaggcatcac ccatccagca ttataaaaca tactaacaat aactaaatta taataatcac
    38581 aacttacaat caaataataa atgcataaga ttaacaatga ttttttcggg ttatatttat
    38641 ctaaaaaagc agaatattat gtaacttatt attttctaag cgaaaattta ttgtgcggtc
    38701 agactaacaa acttgcgtac cacccgaaga acatgcgaag atttaagtat tctttctttt
    38761 ttggttttta tttttcattt tctcttttct tctctttccg gttctgttct ggttttggtt
    38821 ttttcctttt tatttttatt ctttcttcag ggttatgcta aaacaccaaa acatttttca
    38881 ccacaaaagc atccaatcat cttgccagac caatcacctt aaccggatac actgccggat
    38941 ataatcccac taacacccag acaccaagca ccatacctga taactaccgt attcaggaag
    39001 gaaatgcgag tgatttcacc gtcaccggca caaccgatcc gccaaaaaga ggcgtaccaa
    39061 tgtcgatata gtcccccgcg cggacaatca cttcgtcaat gactgccaac gggagttgtt
    39121 gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa gtcctgcccg gccaccacca
    39181 gcaggggatg cggattcgga aaacgcgcga cgaaatcgac cagcgcgttg atgaccgtca
    39241 gaaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc cagcacgtac acgtcagttt
    39301 tgggatcaag atccagctga agcagcgcct gttgccaggc actcaccaga tccgtttcat
    39361 caatcgggat cgccaccggc aaattgcgca gcggcagttg tacgccctcc agccagattg
    39421 tgctgccaga gagcgaaagg gtatgtgcac ccgcgccaat caccgtggag cgtacggttt
    39481 gcgccggaaa ctgcacattc atctcacgca ggcgcggatg gtcatgcagc gccgttgcca
    39541 gaagtgggcc aatatcggca aaacagaacg ggttggcggg ctggtggcga taacattcgc
    39601 ccacgcctcc agaaagcgta atgatttcgg gcgtaacacc tgcgggcagc aaaccggttt
    39661 gcatcaatgc ctgcgcgagc ggcgaaagcg ttccgtcaat cacttcgaca atcagcgccg
    39721 ccatccgccg ggtaacctgc accagctgcg cgccggtcag cgaacgggcg tcagtgcctg
    39781 caccgaagca ctcatccaca atcatctgcc ccggtttatg agcgtaaacc acgcgcccct
    39841 ggctgtcggt ttccagcagg cgaccaccga cgttgagaca ggcagtgccg ctgatttttc
    39901 cggcatcgaa cagggcgtag ttcgcggtgc caccgccgat atcgatattc agtacccgac
    39961 acagccgttg ttcagaaagg gtttgcgccc cggctccgtg accggcgatc acggattcga
    40021 ggtgcggccc ggcgctggcg acgacaaaat cgcccagcga ctgtttttcc ttcatcactt
    40081 tttccatcag ctgtgcgacc aggctttcgg taaactggcc ttccggcagc tgggcaatga
    40141 tggtttcgcg gatgcgctgg ctttcggttt tgtcggctgg cacgctggcg ggcacggacg
    40201 ccgttaccgg gatcgattca tcacattcgg tgatggtaaa acccagtaga tccgccactt
    40261 cacgggcttc cggggtaata atgctggcgc gcagaaccac ggacattgcc tgttcgccgc
    40321 gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt tttcacctgc ttcctcctgt
    40381 ttggtgagag atgccagata atccaccagt tgctgcacgc tttgcgggtc gtggctgttt
    40441 agctcgaaaa tcggctcttc gaaacccgtc tcaagcaata actttcgcgt tgcggcgacg
    40501 tcagcatctg gcatgtccgt tttactgatg acggcgattt gccgtttgct gacgccaata
    40561 tccagcaacc cggcaggcaa ccgactttcc gggtcatttg cgccatggac atagatcagc
    40621 atgtctacat cctgtagcgt ggtaattaag gcgtgatacc agcggggatg gctaaaatat
    40681 tcacccggcg tatcaatatc gcccttatca ttaaattcca cggccagtgt ttttctggcg
    40741 agggtataat tcccctgcaa cgcattaaat aacgttgttt ttcctgcccc gacgctaccc
    40801 acaaacgcaa ttcgtttcat gaatg
//
LOCUS       DAECNO010000028        39855 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.028, whole genome shotgun sequence.
ACCESSION   DAECNO010000028 DAECNO010000000
VERSION     DAECNO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.028"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..157
                     /locus_tag="KKO08_001496"
     CDS             <1..157
                     /locus_tag="KKO08_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6317796.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            386..976
                     /gene="uidR"
                     /locus_tag="KKO08_001497"
     CDS             386..976
                     /gene="uidR"
                     /locus_tag="KKO08_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBD6317797.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1364..3037
                     /gene="uidA"
                     /locus_tag="KKO08_001498"
     CDS             1364..3037
                     /gene="uidA"
                     /locus_tag="KKO08_001498"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBD6317798.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3073..4098
                     /gene="uidB"
                     /locus_tag="KKO08_001499"
                     /pseudo
     CDS             3073..4098
                     /gene="uidB"
                     /locus_tag="KKO08_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4137..5402
                     /gene="uidC"
                     /locus_tag="KKO08_001500"
     CDS             4137..5402
                     /gene="uidC"
                     /locus_tag="KKO08_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBD6317799.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5628..7136)
                     /locus_tag="KKO08_001501"
     CDS             complement(5628..7136)
                     /locus_tag="KKO08_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD6317800.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7237..8412)
                     /gene="manA"
                     /locus_tag="KKO08_001502"
     CDS             complement(7237..8412)
                     /gene="manA"
                     /locus_tag="KKO08_001502"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBD6317801.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8611..10257
                     /gene="fumB"
                     /locus_tag="KKO08_001503"
     CDS             8611..10257
                     /gene="fumB"
                     /locus_tag="KKO08_001503"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD6317802.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10400..11803
                     /gene="fumC"
                     /locus_tag="KKO08_001504"
     CDS             10400..11803
                     /gene="fumC"
                     /locus_tag="KKO08_001504"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBD6317803.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11800..12729)
                     /gene="tus"
                     /locus_tag="KKO08_001505"
     CDS             complement(11800..12729)
                     /gene="tus"
                     /locus_tag="KKO08_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBD6317804.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="KKO08_001506"
     CDS             complement(12805..14106)
                     /gene="rstB"
                     /locus_tag="KKO08_001506"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBD6317805.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14285..15604)
                     /locus_tag="KKO08_001507"
     CDS             complement(14285..15604)
                     /locus_tag="KKO08_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBD6317806.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPQLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15829..16572)
                     /gene="rstA"
                     /locus_tag="KKO08_001508"
     CDS             complement(15829..16572)
                     /gene="rstA"
                     /locus_tag="KKO08_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBD6317807.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16701..17036
                     /locus_tag="KKO08_001509"
     CDS             16701..17036
                     /locus_tag="KKO08_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBD6317808.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17033..17755)
                     /gene="folM"
                     /locus_tag="KKO08_001510"
     CDS             complement(17033..17755)
                     /gene="folM"
                     /locus_tag="KKO08_001510"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBD6317809.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17792..19174)
                     /locus_tag="KKO08_001511"
     CDS             complement(17792..19174)
                     /locus_tag="KKO08_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6317810.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19360..20304)
                     /locus_tag="KKO08_001512"
     CDS             complement(19360..20304)
                     /locus_tag="KKO08_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6317811.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWEKRGNNLTVSADLYK"
     gene            20828..22360
                     /gene="pntA"
                     /locus_tag="KKO08_001513"
     CDS             20828..22360
                     /gene="pntA"
                     /locus_tag="KKO08_001513"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBD6317812.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22371..23759
                     /gene="pntB"
                     /locus_tag="KKO08_001514"
     CDS             22371..23759
                     /gene="pntB"
                     /locus_tag="KKO08_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBD6317813.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23784..24818)
                     /gene="tqsA"
                     /locus_tag="KKO08_001515"
     CDS             complement(23784..24818)
                     /gene="tqsA"
                     /locus_tag="KKO08_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBD6317814.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25229..25594
                     /gene="mdtJ"
                     /locus_tag="KKO08_001516"
     CDS             25229..25594
                     /gene="mdtJ"
                     /locus_tag="KKO08_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBD6317815.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25581..25910
                     /gene="mdtI"
                     /locus_tag="KKO08_001517"
     CDS             25581..25910
                     /gene="mdtI"
                     /locus_tag="KKO08_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBD6317816.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25949..26770)
                     /locus_tag="KKO08_001518"
     CDS             complement(25949..26770)
                     /locus_tag="KKO08_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBD6317817.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26870..26953)
                     /locus_tag="KKO08_001519"
     CDS             complement(26870..26953)
                     /locus_tag="KKO08_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317818.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27046..27381)
                     /locus_tag="KKO08_001520"
     CDS             complement(27046..27381)
                     /locus_tag="KKO08_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBD6317819.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27949..28206)
                     /locus_tag="KKO08_001521"
     CDS             complement(27949..28206)
                     /locus_tag="KKO08_001521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317820.1"
                     /translation="MVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMPFGLL
                     MTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG"
     gene            complement(28222..29031)
                     /locus_tag="KKO08_001522"
     CDS             complement(28222..29031)
                     /locus_tag="KKO08_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6317821.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSP"
     gene            29138..30040
                     /locus_tag="KKO08_001523"
     CDS             29138..30040
                     /locus_tag="KKO08_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6317822.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30175..31395
                     /gene="mlc"
                     /locus_tag="KKO08_001524"
                     /gene_synonym="dgsA"
     CDS             30175..31395
                     /gene="mlc"
                     /locus_tag="KKO08_001524"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBD6317823.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31520..32215
                     /gene="bioD"
                     /locus_tag="KKO08_001525"
     CDS             31520..32215
                     /gene="bioD"
                     /locus_tag="KKO08_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD6317824.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32168..33460)
                     /gene="clcB"
                     /locus_tag="KKO08_001526"
     CDS             complement(32168..33460)
                     /gene="clcB"
                     /locus_tag="KKO08_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBD6317825.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33619..34233)
                     /gene="dmsD"
                     /locus_tag="KKO08_001527"
     CDS             complement(33619..34233)
                     /gene="dmsD"
                     /locus_tag="KKO08_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBD6317826.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34276..35130)
                     /locus_tag="KKO08_001528"
     CDS             complement(34276..35130)
                     /locus_tag="KKO08_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD6317827.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35132..35749)
                     /gene="dmsB"
                     /locus_tag="KKO08_001529"
     CDS             complement(35132..35749)
                     /gene="dmsB"
                     /locus_tag="KKO08_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD6317828.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35760..37798)
                     /locus_tag="KKO08_001530"
                     /pseudo
     CDS             complement(35760..37798)
                     /locus_tag="KKO08_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37859..38968)
                     /locus_tag="KKO08_001531"
     CDS             complement(37859..38968)
                     /locus_tag="KKO08_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD6317829.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38951..39748
                     /locus_tag="KKO08_001532"
                     /pseudo
     CDS             38951..39748
                     /locus_tag="KKO08_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT        10141 a         9660 c        10202 g         9852 t
ORIGIN      
        1 aattgaacaa aaaatgttac agcacacgcc gatcagacgt ctgggccaac cgcaagatat
       61 tgctaacgcg gcgctgttcc tttgctcgcc tgcagccagc tgggtaagcg gacaaattct
      121 caccgtctcc ggtggtgggg tacaggagct caattaatac actaacgaac cggtaaacgg
      181 tcgtatgtgt tgtttaccgg gatagcgtgt gcaattcatc aacgtctctg ctaaacaact
      241 ggcagccaaa tcacggctat tggttaacca attgctgggt gaaaagtata cgaatagagt
      301 gtaccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg gtaaactagt
      361 tattcacgct aactataatg gtttaatgat ggataacatg cagactgaag cacaaccgac
      421 acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat ttcacagtgc
      481 ctcgatgaaa gccatctgta aatcttgcgc cattagtcct gggacgctct atcaccattt
      541 catctccaaa gaagccttga ttcaggcgat tatcttacag gaccaggaga gggcgctggc
      601 tcgtttccgg gaaccgattg aagggattca ttttgttgac tatatggtcg agtctattgt
      661 ctctctcacc catgaagcct ttgggcaacg ggcgctggtg gtcgaaatta tggcggaagg
      721 gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga tcactgaatt
      781 tgttgcccag cggatgcgtg atgcccagca aaaaggcgag ataagcccag acatcaacac
      841 ggcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg atatcgaagc
      901 ggaagacctg gcgcgtgaag cgtcgtttgc tcagggatta cgcgcgatga ttggcggtat
      961 attaaccgca tcctgatcct ctcatttcgc gggctggtga taactgtgcc cgcgtttcat
     1021 atcgtaattt ctctgtgcaa aaattatcct ccccgtgttc ggagaattcc cccccaaaat
     1081 attcaccgta gccatatgtc atgagagttt atcgttccca atactctcga acgaacgttc
     1141 gattgcttat tttatggctt ctgtcaacgc tgttttaaag attaatgcga tccatatcac
     1201 gctgtgggta ttgcagtttt tggttttttg atcgcggtgt cagttctttt tatttccatt
     1261 tctcttccat gggtttctca cagataactg tgtgcaacac agaattggtt aactaatcag
     1321 attaaaggtt gaccagtatt tttatcttaa tgaggagtcc cttatgttac gtcctgtaga
     1381 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga
     1441 aaactgtgga attgatcagc gttggtgcga aagcgcgtta caagaaagcc gggcaattgc
     1501 agtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt atgtgggcaa
     1561 cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct
     1621 gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga
     1681 gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa
     1741 aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg
     1801 aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa
     1861 ctatgccggg atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga
     1921 tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt
     1981 ggtggccaat ggtgatatca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac
     2041 tggacaaggc accagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga
     2101 aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc
     2161 gctgcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa
     2221 accgttctac tttactggct ttggtcgtca tgaagatgcg gatttgcgag gcaaaggatt
     2281 cgataacgtg ctgatggtgc acgatcacgc attaatggac tggattgggg ccaactccta
     2341 ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat
     2401 cgtggtgatt gatgaaactg cagctgtcgg ctttaacctc tctttaggca ttggtttcga
     2461 agcgggcaac aagccgaaag aactgtacag cgaagaggct gtcaacgggg aaactcagca
     2521 ggcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt
     2581 gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc
     2641 gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt
     2701 aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa
     2761 ccgttattac ggttggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga
     2821 aaaagaactg ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg
     2881 cgtggatacg ttagccggcc tgcactcaat gtacaccgac atgtggagtg aagagtatca
     2941 gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga
     3001 acaggtatgg aatttcgccg attttgcgac ctcgtaaggc atattgcgcg ttggcggtaa
     3061 caattgacct acatgggcct cgggctttgc tacagcctgg tgaatattcc ttatggttca
     3121 cttgctaccg cgatgaccca acaaccacaa tctcgcgccc gtctgggcgc ggctcgtggg
     3181 attgccgctt cattgacctt tgtatgcctg gcatttctga taggaccgag cattaagaac
     3241 tccagcccgg aagagatggt gtcggtatac catttctgga cgattgtgct ggtgattgcc
     3301 ggaatggtgc tttacttcat ctgcttcaaa tcgacgcgtg aaaatgtggt acgtatcgtg
     3361 gcgcagccgt cattgaagat cagtttgcaa accctgaaac ggaatcgccc gctgtttatg
     3421 ttgtgcatcg gtgcgctgtg tgtgctgatt tcgaccttcg cggtcagcgc ctcgtcgttg
     3481 ttctacgtgc gctatgtgtt aaatgatacc gggctgttca ctgtgctggt actggtgcaa
     3541 aacctggttg gtactgtggc atcggcaccg cttgtgccgg ggatggtcgc gaggatcggt
     3601 aaaaagaata ccttcctgat tggcgctttg ctgggaacct gcggttatct gctattcttc
     3661 tgggtttccg tctggtcact gccggtggcg ttggttgcgt tggccatcgc ttcaattggt
     3721 cagggcgtta ctatgactgt gatgtgggca ctggaagctg ataccgtaga atacggtgaa
     3781 tacctgaccg gcgtgcgaat tgaagggctc acctattcac tattctcatt tacccgtaaa
     3841 tgcggtcagg caatcggagg ttcaattcct gcctttattt tgggattaag cggatatatc
     3901 gccaatcagg tgtaaacgcc ggaagttatt atgggcatcc gcacatcaat tgccttagta
     3961 ccttgcggat ttatgctact ggcattcgtt attatctggt tttatccgct cacggataaa
     4021 aaattcaaag aaatcgtggt tgaaattgat aatcgtaaaa aagtgcagca gcaattaatc
     4081 agcgatatca ctaattaata ttcaataaaa ataatcagaa catcaaaggt gcaactatga
     4141 gaaaaatagt ggccatggcc gttatttgcc tgacggctgc ctctggcctt acctctgctt
     4201 acgcggcgca actggctgac gatgaagcgg gactacgcat cagactgaaa aacgaattgc
     4261 gcagggctga taagcccagt gctggcgcgg gaagagatat ttacgcatgg gtacagggag
     4321 gattgctcga tttcaatagt ggttattatt ccaatattgt tggcgttgaa ggcggagcgt
     4381 attatgttta taaattaggt gctcgtgctg atatgagtac ccggtggtat cttgatggtg
     4441 ataaaagttt tggctttgcc ctgggggcag taaaaataaa acccagtgaa aatagcctgc
     4501 ttaaattagg tcgcttcggg acggattata gttatggtag cttaccttat cgtattccgt
     4561 taatggttgg cagttcgcaa cgtacattac cgacagtttc tgaaggagca ttaggttatt
     4621 gggctttaac gccaaatatt gatctgtggg gaatgtggcg ttcacgagta tttttatgga
     4681 ctgattcaac aaccggtatt cgtgatgaag gggtgtataa cagccagacg ggaaaatacg
     4741 ataaacatcg cgcacgttct tttttagccg ccagttggca tgatgatacc agtcgctatt
     4801 ctctgggggc atcggtacag aaagatgttt ccaatcagat acaaagtatt ctcgagaaaa
     4861 gcataccgct cgacccgaat tatacgttga aaggggagtt gctcggcttt tacgcgcagc
     4921 tcgaaggttt aagtcgtaat accagccagc ccaatgaaac ggcgttggtt agtggacaat
     4981 tgacctggaa tgcgccgtgg ggaagcgtat ttggcagtgg tggttatttg cgccatgcaa
     5041 tgaatggtgc cgtggtggat accgacattg gctatccctt ttcattaagt cttgatcgta
     5101 accgtgaagg aatgcagtcc tggcaattag gtgccaacta ccgtgtaact ccgcaattta
     5161 cgctgacatt tgcaccgatt gtgactcgcg gctatgaatc cagtaaacga gatgtgcgga
     5221 ttgaaggcgc gggtatctta ggtggtatga actatcgggt cagcgaaggg ccgttacaag
     5281 ggatgaattt ctttcttgct gccgataaag ggcgggaaaa gcgcgatggc agtacgctgg
     5341 gcgatcgcct gaattactgg gatgtgaaaa tgagtattca gtatgacttt atgctgaagt
     5401 aagaaataac gccggagaga aaaacctccg gcgtttcaga ttgttgacaa agtgcgcgtt
     5461 ttttatgccg gatgcggcgt aaacgcctta tccagcctac aaaaactcat aaattcaaag
     5521 tgttgcagga aaaggtaggc ctgataagcg tagcgcatca ggcaatctct ggtttgtttt
     5581 cagatgaaaa cgccggagtg aaaattctcc ggcgttttgg ccgtgaatta ctgctgcgga
     5641 attgccggta cagccggaac gttaagagct ggcatcgcaa acatgccaac aaaatcttct
     5701 aacgacattt tctgcccatt taacgttatc tgaccgttag catattgcag gctggtggtg
     5761 atggtattgt cctgcaaggt ggtcagacgg aacatctgcc ccattgctga tgcaccttca
     5821 acttgctgtt tcgccagttt tttcgcttga tcttcctgat aaccttccag cttcgctacc
     5881 tgagtcatca actcagttgc catatccacc ggaatggtca gtttcgcatc cagagattta
     5941 accgaacgat ctacttcctg cgccagcgtt tgcggcgctt ctttagtcgt tgccggatct
     6001 ttcaggaaca gcgacagatt cagcgcactt tcaccctgac tgtttttcca gcttagcggc
     6061 gcgatagtaa tcaccggatc gcctttcaac atcagcggca gggcgctaaa gaaggcttcc
     6121 gtcactttct cctgataaag ttcgggattg ttggcaattt ctggctgtgc cagcagcgcc
     6181 tgagtttgcg cgttatattg ctggctaaac tgatgccagg cttcaccatc aatctggccg
     6241 acttttaaag tcagcttgcc gctgcccaga tcctgattct gtaccttcag gctgtttagc
     6301 gagtaatcca gttggctatt gatcgtttta ccgtcattga ccagatccga tttaccgctg
     6361 atctccatgc cttccagcag tgccagttct ttgccttcca ctgaaatggt cattttttcc
     6421 agtgacagtt tttggtttcc tacgcgctca ccaaaacttc ccagcgtgct ggaaccgtcg
     6481 gttttcagat tattaaaggt caactgcact ttctggttgt attcgttaac tgcgtctatc
     6541 cgaccacttt gcgcctctcc ggaaagggag atggctttgc cgtctctgtc agcatttaac
     6601 tggaactcgc cgccgctaaa ggcgactttt tcatcctttt gctcgtaatt cagtggattg
     6661 agcgaaatat cggaactgga atcaccgctg taaccaatgc gcgagttaat ctcaaaaggc
     6721 gtttcacctt ttgccatatc aaacagtggt ttgcttactt cgttattaac cagcgtggtt
     6781 tgaattgatg ccatcgacgg gatcaggttc agttttttaa gctgggcaag cgggaaggga
     6841 ccatgatcaa ccgattcgtt gaagatgacg ctctgaccgc ttttaatcca cggattttct
     6901 ttcccggcaa tgggtttcac caacagttgc aactggctgc tgaatacgcc gcgatgatag
     6961 ttttgataac tcacttccag gttggattca ggagctgtca gtttgagttg cgcgttcgcc
     7021 tgcgcgacca tgtcttcgag atgggtttca atcttcttgc ctgtatacca tgcgccgcct
     7081 gtccagacta cgcctagcgc aacaatgacg cctaccgcta ccagcgattt attcataatg
     7141 attatccata aaatgaaatc aggcggactg gccgcctgaa ggtgttataa gcctttaata
     7201 agcttagcaa gagatgttaa ttttttcagt aagctcttac agcttgttgt aaacacgcgc
     7261 taaacggccg tggcctttga cagtcaccgg tgattcgttg gcggcaataa acgctgattc
     7321 acccggttta agctgtaact gctgagaacc tttccacaac gttgcatcgc cttcgacgca
     7381 gaacaaaatg gcggcactct gctggctgat ggtggtttct ttatcactaa ggtcatgcag
     7441 cgagaaggca aaatcatcca caggaatcgg gaagtccagt tctgcacctt gtttcaccgg
     7501 ctgggtcaac aactggttag ccggtttggc ttcgaatttc acattggcaa ccagttccgg
     7561 aatatcaatg tatttaggcg tcagacctgc acgcagcacg ttatcggagt tcgccatcac
     7621 ttccagcgcc acgccttgca ggtaagcgtg cggtgtttca gcgaacagga acatcgcttc
     7681 gccaggattc aatttcacca cattcagtaa cagcggggag aacaggccgc tgtcttccgg
     7741 gtaaaattca gaaattaaac gaatcgtttg ccacggttca ccctgctgac tgtcgagcgc
     7801 cgattttaaa atcgccagcg cgcgggattt ttcttcaccc tgcatattca acaggttggc
     7861 gaacagttcg cttaaacgtt cggcatgtgg ctgttgtaaa aagtgagcaa tcgccggatg
     7921 tgcaccggcg accggctgga gtagggagac aatctcggaa aattcacgaa acgcgttcat
     7981 cgcaaggaaa ggcgtcagcg caaaaaccag ctccggcttg tggttaggat ctttatagtt
     8041 acgctcggcg gcatccatcg ggatacctgc ggcattttct ttggcaaaac cgatttcaga
     8101 attgtgtttg tttggatgaa cctgaatgga gagtggctgt gctgcgcata atactttgaa
     8161 caggaaaggc agttcgccaa agcgtttggc aacggcctct ccgagcagag tcgatttatc
     8221 actctcaatc acatcacgca gtgaaacgat atctccggcg gcattctgca ctcgtgaact
     8281 gcttttcgga tgtgcgccca tccacagctc ggccatcggc tggctggacg gattttccat
     8341 accataaagt tcagtcaacg ccgttttgct accccaggca tagttttgca ctgagttaat
     8401 gagtttttgc atgatcaatc cctgttttaa tgtggaaatt aatcccgcta ttaaagcaag
     8461 aatcctacgg gaagtaacct ggagccgcaa aaagtcgtac tagtctcagt ttttgttaaa
     8521 aaactgtgta ggatattgtt actcgctttt aacagggcaa cggaacaccc gcccagagca
     8581 taaccaaacc aggcagtaag tgagagaaca atgtcaaaca aaccctttca ttatcagtct
     8641 ccttttccac tcaaaaaaaa tgatactgag tattacctgc taaccagtga acacgttagc
     8701 gtatctgaat ttgaagggca ggagattttg aaagtcgcac ccgaagcgtt aactctgttg
     8761 gcgcgtcagg cgtttcatga tgcgtcgttt atgctgcgtc cggctcacca acaacaggtg
     8821 gccgacattc tgcgtgaccc ggaggccagc gaaaatgata aatatgtggc gctgcaattc
     8881 ctgcgtaact ccgacatcgc ggcgaaaggc gttctgccaa cctgtcagga taccggcacc
     8941 gcgattattg ttggtaaaaa agggcagcgt gtatggaccg gtggtggtga tgaagcggcg
     9001 ctggcgcgcg gtgtctataa cacttatatc gaagataatc tgcgctactc gcaaaacgcg
     9061 ccgctggata tgtataaaga agtgaatacc ggcaccaatc tgccagcgca gatcgatctt
     9121 tatgccgttg atggcgacga atacaaattc ctctgtatcg caaaaggtgg tggttcggca
     9181 aacaagacgt atctctatca ggaaaccaaa gcgttactga cgccggggaa actgaaaaat
     9241 tacctggttg agaagatgcg cacgctgggt accgcggcct gtcctccgta tcatattgcg
     9301 ttcgttattg gtggaacttc tgcagaaacg aaccttaaaa cggtgaaact ggcttccgcg
     9361 aaatactatg atgaactgcc aacggaaggg aatgagcacg gtcaggcgtt ccgcgatgtg
     9421 gaactggaaa aagaattact gatcgaagcg caaaatctcg gtctgggtgc gcagtttggt
     9481 ggtaaatact tcgctcacga catccgcgtg attcgcctgc cacgtcacgg cgcatcctgt
     9541 ccggttggta tgggtgtttc ctgctctgct gaccgtaata tcaaagcgaa gatcaaccgt
     9601 caggggatct ggatcgaaaa actggaacat aatccaggta aatatatccc tgaagagctg
     9661 cgtaaagcgg gagaaggcga agcggtgcgc gttgacctta accgtccgat gaaagagatc
     9721 ctcgcacagt tgtcgcagta tcccgtttct acacgcttat cgcttaacgg cacgattatc
     9781 gttggtcgtg atattgctca cgccaaactg aaagagcgga tggataacgg tgaagggctg
     9841 ccgcaataca ttaaagatca tccgatttac tacgcgggtc cggccaaaac gccggaaggt
     9901 tatgcctccg gttctcttgg cccaacgacc gccggacgga tggattctta tgtcgatcaa
     9961 ctgcaagcgc agggcggaag tatgatcatg ctggcgaaag gcaaccgcag ccagcaggtg
    10021 acggatgcct gtaaaaaaca cggcggcttc taccttggca gtatcggtgg tccggccgct
    10081 gtattggcgc agggaagtat taagagcctg gaatgtgttg aatatccgga actgggaatg
    10141 gaagccatct ggaaaattga agtggaagat ttcccggcgt ttatccttgt ggatgataaa
    10201 ggaaatgact tcttccagca gatacaactc acacagtgca ctcgctgtgt gaaataaaca
    10261 gagccgccct tcggggcggt ttttttacat ggcacgaaag accaaacatt tgttatcaaa
    10321 tagtaaataa tgagtgagct aaaagttgct taacgaaagc aaaacagaaa gaaaaaatta
    10381 atcaggtgag gagcaggcca tgaatacagt acgcagcgaa aaagattcga tgggggcgat
    10441 tgatgtcccg gcagataagc tgtggggcgc acaaactcaa cgctcgctgg agcatttccg
    10501 catttcgacg gagaaaatgc ccacctcact gattcatgcg ctggcgctaa ccaagcgcgc
    10561 agcggcaaaa gttaatgaag atttagactt gttgtctgaa gagaaagcga gcgccattcg
    10621 gcaggcggcg gatgaagtac tggcaggaca gcatgacgac gaattcccgc tggctatctg
    10681 gcagaccggc tccggcacgc aaagtaacat gaacatgaac gaagtgctgg ctaaccgggc
    10741 cagtgaatta ctcggcggtg tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt
    10801 gaacaaaagc caaagttcca acgatgtctt tccgacggcg atgcacgttg cggcgctgct
    10861 ggcactgcgc aagcaactca ttccgcagct taaaaccctg acacagacgc tgagtgaaaa
    10921 atcgcgtgca tttgccgata tcgtcaaaat cggtcgaacc cacttgcagg acgccacgcc
    10981 gctaacacta gggcaggaga tttccggctg ggtagcgatg ctcgagcata atctcaaaca
    11041 tatcgaatac agcctgcctc acgtagcgga actggctctt ggcggtacag cggtgggtac
    11101 tggactaaat acccatccgg agtatgcgcg tcgcgtagca gatgaactgg cagtcattac
    11161 ctgtgcaccg tttgttaccg cgccgaacaa atttgaagcg cttgcgacct gtgatgccct
    11221 ggttcaggcg cacggcgcgt tgaaagggtt ggctgcgtca ctgatgaaaa tcgccaatga
    11281 tgtccgctgg ctggcctctg gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa
    11341 tgagccgggc agctcaatca tgccggggaa agtgaaccca acccagtgtg aagcattaac
    11401 catgctctgc tgtcaggtaa tggggaacga cgtggcgatc aacatgggtg gcgcttccgg
    11461 taactttgaa ctgaacgtct tccgtccgat ggtgatccat aatttcctgc aatcggtgcg
    11521 cttgctggca gatggcatgg aaagttttaa caaacactgc gcagtgggta ttgaaccgaa
    11581 tcgtgagcga atcaatcaat tactcaatga atcgctgatg ctggtgactg cgcttaacac
    11641 ccacattggt tatgacaaag ccgcggagat cgccaaaaaa gcgcataaag aagggctgac
    11701 cttaaaagct gcggcccttg cgctggggta tcttagcgaa gccgagtttg acagctgggt
    11761 acggccagaa cagatggtcg gcagtatgaa agccgggcgt taatctgcaa catacaggtg
    11821 cagccgtgga atgatcaaac gaagcggctg cgcctgaggt ttataacggt gctgcacatt
    11881 gtcggcatcg taatttaaca actcaccaac gtccggcacg cccgcgccat tatcccgatt
    11941 aatcagtgca atcagtggtg tagggcaggc gtgttggact tgtttttgat ctcctttgta
    12001 ccagacgcgg gcaatcggct gcaccttcac tggacgtttg atttttaact tcgcgttctg
    12061 tggcagggca gcgatatcct gatactctcg ctccagtttt ctttgccact cctcgcgcgt
    12121 ccacggtgcg acactgcgtg gtgatttcag gcttttttcc agctgtgcca ggacttcatc
    12181 acgatgcaaa ttcttaatga tatgtttatt agcccaacca aagcgtaaag tggcggggtc
    12241 gtgcagaacg gtgagcgtgc ggtaagcatt aagggtgatc agccccggca aatgacgatg
    12301 cacccattca aaacgtgccg cggtggggag ttctgattca accgtgacga tatgctcgaa
    12361 cgtggtcttg agtttattga tgtgctgaat atgactgacc aacgctgctt gcgaaaggtt
    12421 atcgacctgg taacacaaca cgccaggcag acggacagcg gccttgctgc tgcgattttc
    12481 ggactgttgt tgaataaata aatggcggaa atgacgcaac gccagcgact gcgcgtcgtt
    12541 gccgagatgt tgttttacct caatacgatt aagcggatta tgctcatcct ctttttttac
    12601 ctccggcaaa gagaacacgc gggcaaccaa tagcttgtgt tgctcaagat gagcggcaaa
    12661 tgtagccagc tcttgttcca tctggcgaaa ggtagtgttg agtcggtcta cgagatcgta
    12721 acgcgccata atattaacca ctttagttac aacatactta ttttatagca cagtcgtggt
    12781 ggcttgacca tcgtgcgcgt agtgtcaggc agaggtaaat tgcgggatgt tatgccataa
    12841 cggccagcta aacgagaagc gagcaccacc cagttcgctg atgtcacaat taaccgtacc
    12901 gcccattgcc agtgctattg agtggacaat tgccagcccc agcccgcagc cgccggttga
    12961 gcgatcccgg ctgggatcga ggcgaacaaa aggttcaaag atatgttcgc ggttttctgg
    13021 ggcaatccct gggccatcat cctcaacaat taatgtcgct ctattccccg acagtagcag
    13081 gctggtttca accgttgaat ggcagtagcg cagggcgtta ttgagcaaat tatccagcac
    13141 gcgctccatt aagcgcatat ccaacgccgc ataatggcct tgcatgagcg tttttatccg
    13201 taccgtttta tcgggcgtta ctgcctgaat atctgccaga tgcgttgaca gccacaacgg
    13261 caggtctggt tcgctaagat gaagctcgtt ttgtgggcga tcgagtcggg cataagtcag
    13321 cagctcttca attaaagctt caagttgact gatatcacga ttcaacgcct gggattcggc
    13381 ggcgctcagg ttatcgctca tctccagtcg ataacgcagg cgcactaacg gtgttcgcag
    13441 ttcgtgagcg ataccgtcaa taagctgttt tttgctggca attaaggcgt tgatattgtc
    13501 cgccatctgg ttaaatgcga taccaagtcg ttcaaagctc gaaccctcat caaagtggat
    13561 acgttcattg agatgcccat cgccaaatcg ttgcgccgct gcttccagtt ttaacatatc
    13621 ctgccagtgc ggacgcatcc agataaacac cggaaaggcg agggaaatag caataaaagc
    13681 gatcagggcg atatccagca atcgcatttg atggaggtaa taaagataag gaacaggacc
    13741 aactgccagc acgtagtggc tgcgcgggat acgctgcaaa aacgtgtact gatcgtccag
    13801 ggcgacaatt tcgccgccac gcagtcggtg catggaaata tcatcaagat ggtatttact
    13861 cagtggctcg acacgcagat cgaaagagag atttaaatcc atctctttca gcgtcttacc
    13921 ccagtcgtgt ggggggatct ctcgcaattc gctgcgcatc agatacagcg aactgttcat
    13981 caaatcatcc agcgactgtt tgcccgcgcg ttcggcggta aatttgtaca ccagcccaac
    14041 cagcagagac atcacaagga agcagacaaa caataacagg taaaactgga taaacaattt
    14101 tttcattggc atcgcccgca acagaatact attcgtatta attcacgact gtggagcaat
    14161 gtaaaccgcc aactggataa gaccttgttt ggaagataat gtccggaaca gacggatgta
    14221 tccccggtgt attcatattc agtaaattca attttacacc ggggcttgtt tattatgcta
    14281 aggtttaatt taacgtgacg ctttgttgtt ctttagtctc tctatcctta agaagacggg
    14341 ctgatattac accggacatt attgaaccat tcacattaag cgctgttctt gccatatcaa
    14401 cgatcggctc tatggcaata aaaataacta ctaatgcgac agggaaatta agtgttgaaa
    14461 ggacaatcag cgccgcgaat gtcccaccac tacctacgcc tgctacccct attgagccaa
    14521 gcgcgatggc aggtaaaaga gttgcgagga attgcaatga gaagggatcg atacccattg
    14581 ttggagcaat catcgccacc atcacggcag gataaatccc agcgcagcca ttttgtccca
    14641 tgctggaacc aaaagacgct gcaatattag caatcgtatt tggaaccccc aatttttcca
    14701 atgcagaaat agccagagga attgaagccg cactactgcg agaaacaaac gcaaaggtaa
    14761 gtacttgcca gacggtcttg taatagttaa cggggttttt acctattaac agcaaaatgc
    14821 ccgagtgaac aataaacatc acaaaaacgg ccagataaca ggcgacgatg aacccgagca
    14881 aattggtaaa ctgtgaaagc tgatacgttg agaagacttt cgccattagt gccattaccc
    14941 cataaggcgt cagcgcaatg acaatacgga ccattttcat tacccatatt tgaatcgcgt
    15001 tgatccccgc gctcaatttt tcgccctctg ctggtgcatc tttctttact tttaacagcg
    15061 caataccagc aagaattgta aagatgacga tgccaataac cgaaacactt cttgccccgg
    15121 tcagatcaag gaagaggttg gttgggatca atgacgtaat gagttgcggt aaagataccg
    15181 cagctgtttt cgagacgtca tcgctggtta aagaggattg catatgacca aaggcgctgg
    15241 catcaaggcc aattgcactg gcaatagcca ggccaactaa acctgcaatc atgactaaac
    15301 acaacataca ggcaacaata gttaaagaca tcttgccaat accagcgcta ttttcgagct
    15361 tattaatcgc tgaaagaatc gaaatgaata tgagtggaac ggcaacaagt tttaataaat
    15421 taacataacc gttaccagca atattatacc attcgataac ggtttcactg cttgtctcag
    15481 gaatgaaatg cagggagata ccaatgatag cccccaggaa aatcgcgata aaaacacttt
    15541 tggaaaggga ttcgctcttt tgatccgttt tgaaaatccc ccatgagcag atcaaaaaaa
    15601 gcacaatata tgtgaaagtt gtaaccgttt tttaagcctc ttgcctcatt cagcacctga
    15661 aatgccggaa agagtttgtt gtaaaaaatc tgcgccataa gacgcaggga cgaggcgatt
    15721 atagagaaat tttttctcat tttcatatca aatgtagggg gtttggaatt tgaataagct
    15781 cttattaatt gaattcgtta attgttctct ttttcagatg aacaaatatt agtcatctga
    15841 ttgtttattg tcactatccc atgcatgagg cgcaaaaaga tagcctttgt tacgcacagt
    15901 tttaatgcga taaggttctg cggcgttatc gagcagtttt tttcttaacc gcgaaatagc
    15961 cacgtccacg ctacgatcca gtccgtcata actgacgccg cgtaaatttt tcagcaatgc
    16021 atcgcggtcc atgatttgcc cggcatgcgt agctagttcc cacaataatt cgaaatcagc
    16081 tgtcgagagc gagatttcag tgttagccag ggtgactacg cggttgatgg ggtcgatagt
    16141 taacgtgccg aaatgcaggg ctttgtaggg agtaagagac gtttcctgaa gacctttggt
    16201 cagtgtggct tgctcattct gacgcaaatg caaacgtaaa cgcgctagca aaacagcagg
    16261 gggcgtcgtt ttgagaatat agtcgcaggc acccatttcc agtgccagga tgtggttcat
    16321 atcgctatcg agagaggtta gaagaacaat cggtccagac cactttgcgc gtaaatcacg
    16381 acaaatggtc atgccgtcct tgcctggtag catgatgtcg agtaacacca aatccggatt
    16441 ttctcgcaaa atggtttctt cggcctggtc gccgcgcggt tctacggtaa cctgcatatc
    16501 atgttttgcc aggtacgcgg caatcagtga accgacttcc gcatcatctt ccacaaatac
    16561 gatagtgttc ataacattca cggtagatat aaaaacgtca caatacaccg cgctgttttt
    16621 acttactatc aatcgtttct aaacaagttt tcatcaggta ttctactgat gacttgttat
    16681 ggaagtgtta aggtaaaaag atggggctgg taatcaaagc cgcgctcggt gcgctggtag
    16741 tgctgttgat tggtgtttta gcaaaaacga aaaattatta tatcgccggg ctgattccac
    16801 ttttcccgac ctttgcgctt atcgcgcatt atattgttgc cagcgaacgc ggcattgaag
    16861 ccttacgcgc aaccatcatt tttagtatgt ggtcgattat tccctatttt gtttacctgg
    16921 tgtcgctgtg gtatttcacc ggaatgatgc gcttgcccgc cgcttttgtt ggttctgttg
    16981 cgtgttgggg gataagcgca tgggtgttga ttatatgctg gataaagctg cattaacgca
    17041 gatgacgacc gccatcaagt gggaaactgc gtccggtgac aaagcaactg gtaagtaagt
    17101 aatcgaccag gtcgatcact tctttctcgc caggcgcggt tttcatcagt gatttattca
    17161 gcgcctgttg tcgatattcg gcatcatcat gttcattaaa caggatcagc gatggcgcaa
    17221 tagaattcac tttcacttcc ggtgccagct tgcgggcaaa cgagcgggtc atattatcca
    17281 gtgccgcttt gcttgcagca tacgcaacat gtttgtcgct accgcgctcc accacataat
    17341 cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc acgcagtaat ctttccagcg
    17401 catggttgag caggtatggg gtattaacgt ggatctgcat catgcaagcc agtacgtcgg
    17461 ccagtggcgc acccggtttt tccgcaatcc acgcactggc gttatgcaaa atagcacgca
    17521 gaccatgggt ggtttttagt acttcatcgg caaacgccat cacaccgtcg ttggtcgaaa
    17581 aatcagcctg aatacactgc gcacctgcat taatcagtcc atcaatggct ggatagtgtg
    17641 tccgatagct gacaatcacc ggttgctttt gattaatgaa atgccatgcg agggcgaggc
    17701 cgatgcgacg acctccgcca gtaattaata ttggcaaggg ctgggtttta cccatcgtta
    17761 tctcctttgc tatccaacga tgggatgaac gttatcccac cagcatccag gtcgccggaa
    17821 ctgaggcaat taacagcatg ccaatcagca ccatttcctg acggttcagt acattatcat
    17881 gtgtatgcgt tttgcgcgca tacaggaaaa ccagcagccc cggtgcatac agaacaacgg
    17941 ataacagcaa gtgcattgga ccagaggcgt acaataacca taagccataa atgcaggcac
    18001 cgacacctac cgctttatgc agtggacggg ttgcgatttt cagcaagaac gcgccgacca
    18061 ggaaataggg caccagaatc atttctgagg cgatggtcag caacgtgttg taatcggaac
    18121 cggtcagcca gatgagtacc agacaaattt gcacgcagat gttggtcaac cacagtgagg
    18181 cggatggcgc agcttgtgcg ttctggcgcg caaaaatgcg tgggaatgct ttatgagtgg
    18241 cagccaggaa cggaacttct gccgccatga tggtccagct taagtacgcg ccgcaaacgg
    18301 aaacgatcaa accggcagcg atgatgattt cgccccatgg tcccatcatt tccaccatca
    18361 gaccggccat tgacgggtta cgaatttcag ccagttcagg acgtgccacc acacccagcg
    18421 aaagcagcgt taccagcaag taaacgccca gagcggagag aaccgccagc agtgtcgctt
    18481 tgccaacatc acgtttatta cgcgcacgcg cagaaacaac cacggcacct tccacaccaa
    18541 tgaaaaccca cagggtgatc agcatggtgt ttttcacctg ttcccaaaca ggtacgccaa
    18601 gtgcaagtcc ggtgaagtcg agcttgaagg tatccagttt gaacatcatc atcgccagca
    18661 caacaaacag acccagcggc aacaattttg ccagtgtcgc caccaggtta atgctggcag
    18721 cggtttgcac cccgcgcagg atcaaaaagt gaacaatcca caataaagcc gatgcaccga
    18781 ctatcgattg ccaggtatta ccatcgccaa acaggcgcaa ttccggcgtg tccgtaaaaa
    18841 agcttaacgc ggaaaaaacg atcaccagat aggagacgtt ggcgatgacc gcgcacagcc
    18901 agtatcccca tgcggaacaa aagccgatta gctcgccaaa cccttcgcgg gcataggtaa
    18961 agataccgcc atcaagttcg ggacgaatgc gcgtgaggat cagcatggca aaggccagca
    19021 ataaaatgcc agcgccagta ataccccagc cgatgagcag tgctgccggg ctggcaactg
    19081 ccgccatatt ttgcggcaga ctgaaaacac ccgcgcccag cattgagctt aataccagcg
    19141 cggtgagtgc gctcagtccc agtttctttt ccatcgatat ccagtgatta aaacgtgata
    19201 agaagaataa atattagggc aaaacgcata aaaatggagt ttgccactga agcgcgggat
    19261 tttacgaaga gatgtgtctg catgcaatgg tcgcggggaa atttgtgact aaaaatgcga
    19321 aaggcggcat caagccgcct tataggagta actgaatagt tatttataca gatctgcgct
    19381 gacggtcagg ttattaccac gtttctccca ctggcgggtg atgtggtaat acttagcacc
    19441 tttcttcgcg gcacgtttcg caacctgata ggagacttcg gtcatgttgc cgtagttgcc
    19501 agagaatttg atgctgtcga acgggaccat catcgctgct gtcgctttgt tcagttcttc
    19561 gactttagtg ccatccggga gcgtgacggt gtaacgcccg ccttttgatg actgggtttc
    19621 aaagaagcga ccgacttcag aacttggtga tgcggtagtc gcaacacccg ggatctcaac
    19681 tttcttcgcg gcttcgccac cggcagccag agctgcacgt cctgcttcgg aatctgccgg
    19741 gatcacatcc gggctctgga cgatacgttt cttagcatct tttttataga tgaatgcagt
    19801 aatacgctgg ttgccgccct ggttggcatc gatttgacga acgatgtaga aagagtaggc
    19861 acctttcgct tttgccgctt tggtgatggc atcattgact tctggctggc tacgatagaa
    19921 gccctggacg gtgaccgtgt caaacggttc aatcagaaca gcctgatctt tcggcagttc
    19981 gacaacaccg ttaattacgc gattacttgt ttcttctgct ttttcagcat cggctttata
    20041 gaggtcagcg accacacgcc agttaccgct gttaccaaaa tcagaagtgt cgacaacata
    20101 aaaagaggcg gcaccttctt tatctgcgcg acgagaaacg gctttcaccg cttcgccaat
    20161 agcattaaaa cgaccggtaa ccactacacg gtcaaaaggt ttaaccgctg ccgcttgctc
    20221 cggtgtcagt tctgttgctg cgttaacgga gaatgccgta gcagaaagca gtgccgacgc
    20281 caggagggtg ttcttaagct tcataaaaat aatccttcgc cttgcgcaaa ccaggtactg
    20341 gtattgttat taacgagaaa cgtggctgat tattgcattt aaacggtgta actgtctgcg
    20401 tcatttttca tatcacattc cttaaaccaa ttttaatcct gctcaaatga ccgtctatgc
    20461 ttaaaaaaca gccgtatcag catcattact actgaagcaa ctgaattgta taagttaatt
    20521 taatgttaag tagtgattcg tgccggggcg atgtctcgtt ttacccgacc gtcgaagaca
    20581 attatcagtc tttatccggc gttctaaggt gtttatccca ctatcacggc tgaatcgtta
    20641 atattttgcg agttcacgcc gaaatactga tttttggcgc tagatcacag gcataatttt
    20701 cagtacgtta tagggcgttt gttactaatt tattttaacg gagtaacatt tagctcgtac
    20761 atgagcagct tgtgtggctc ctgacacagg caaaccatca tcaataaaac cgatggaagg
    20821 gaatatcatg cgaattggca taccaagaga acggttaacc aatgaaaccc gtgttgcagc
    20881 aacgccaaaa acagtggaac agctgctgaa actgggtttt accgtcgcgg tagagagcgg
    20941 cgcgggtcaa ctggcaagtt ttgacgataa agcgtttgtg caagcgggcg ctgaaattgt
    21001 agaagggaat agcgtctggc agtcagagat cattctgaag gtcaatgcgc cgttagatga
    21061 tgaaattgcg ttactgaatc ccgggacaac gctggtgagt tttatctggc ctgcgcagaa
    21121 tccggaatta atgcaaaaac ttgcggaacg taacgtgacc gtgatggcga tggactctgt
    21181 gccgcgtatc tcacgcgcac aatcgctgga cgcactaagc tcgatggcga atatcgccgg
    21241 ttatcgcgcc attgttgaag cggcacatga atttgggcgc ttctttaccg ggcaaatcac
    21301 tgcggccggg aaagtgccac cggcaaaagt gatggtgatt ggtgccggtg ttgcaggttt
    21361 ggccgccatt ggcgcagcaa acagtctcgg cgcgattgtg cgtgcattcg acacccgccc
    21421 ggaagtgaaa gaacaagttc aaagtatggg cgcggaattc ctcgagctgg attttaaaga
    21481 ggaagcgggc agcggcgatg gctatgccaa agtgatgtcg gacgcgttca tcaaagcgga
    21541 aatggaactc tttgccgccc aggcaaaaga ggtcgatatc attgtcacca ccgcgcttat
    21601 tccaggcaaa ccagcgccga agctaattac ccgtgaaatg gttgactcca tgaaggcggg
    21661 cagtgtgatt gtcgacctgg cagcccaaaa cggtggcaac tgtgaataca tcgtgccggg
    21721 tgaaatcttc agtacggaaa atggtgtcaa agtgattggt tataccgatc ttccgggccg
    21781 tctgccgacg caatcctcac agctttacgg tactaacctc gttaatctgc tgaaactgtt
    21841 gtgcaaagag aaagacggca atatcactgt tgattttgat gatgtggtga ttcgcggcgt
    21901 gaccgtgatc cgtgcgggcg aaattacctg gccggcaccg ccgattcagg tatcagctca
    21961 gccgcaggcg gcacaaaaag cggcaccgga agtgaaaact gaggaaaaat gtgcctgctc
    22021 accgtggcgt aaatacgcgt tgatggcgct ggcaatcatc ctgttcggtt ggctgtcaag
    22081 cgttgcgccg aaagagtttc ttgggcactt caccgttttc gcgctggcct gcgttgtcgg
    22141 ttattacgtg gtgtggaatg tatcgcacgc gctgcataca ccgttgatgt cggttaccaa
    22201 cgcgatttca gggattattg ttgtcggagc actgttgcag attggccagg gaggctgggt
    22261 tagcttcctt agttttatcg cggtgcttat agccagcatt aatattttcg gtggcttcac
    22321 cgtgactcag cgcatgctga aaatgttccg caaaaattaa ggggtaacat atgtctggag
    22381 gattagttac agctgcatac attgttgccg cgatcctgtt tatcttcagt ctggccggtc
    22441 tttcgaaaca tgaaacgtct cgccagggta acaacttcgg tatcgccggg atggcgattg
    22501 cgttaatcgc aaccattttt ggaccggata cgggtaatgt tggctggatc ttgctggcga
    22561 tggtcattgg tggggcaatt ggtatccggc tggcgaagaa agttgaaatg accgaaatgc
    22621 cagaactggt ggcgatcctg cacagcttcg tgggtctggc ggcagtgctg gttggcttta
    22681 acagttatct gcatcatgac gcgggaatgg caccgattct ggtcaatatt cacctgacgg
    22741 aagtgttcct cggtatcttc atcggggcgg taacgttcac gggctcggtg gtggcgttcg
    22801 gcaaactgtg tggcaagatt tcgtctaaac cgttgatgct gccaaaccgt cacaaaatga
    22861 acctggcggc tctggtcgtt tccttcttgc tgctgattgt atttgttcgc acggacagcg
    22921 tcggcctgca agtgctggca ttgctgataa tgaccgccat tgcgctggta ttcggctggc
    22981 atttagtcgc ctccatcggt ggtgcagata tgccggtggt ggtgtcgatg ctgaactcgt
    23041 actccggctg ggcggctgcg gctgcgggct ttatgctcag caacgacctg ctgattgtga
    23101 ccggtgcgct ggtcggttct tcgggggcta tcctttctta cattatgtgt aaggcgatga
    23161 accgttcctt tatcagcgtt attgcgggtg gtttcggcac cgacggctct tctactggcg
    23221 atgatcagga agtgggtgag caccgcgaaa tcaccgcaga agagacagcg gaactgctga
    23281 aaaactccca ttcagtgatc attactccgg ggtacggcat ggcagtcgcg caggcgcaat
    23341 atcctgtcgc tgaaattacc gagaaactcc gcgctcgtgg tattaatgtg cgtttcggta
    23401 tccacccggt tgcggggcgt ttgcctggac atatgaacgt attgctggct gaagcaaaag
    23461 taccgtatga catcgtgctg gaaatggacg agatcaacga tgactttgct gataccgata
    23521 ccgtactggt gattggtgct aacgatacgg ttaacccggc ggcgcaggat gatccgaaga
    23581 gtccgattgc tggtatgcct gtgctggaag tgtggaaagc gcagaacgtg attgtcttta
    23641 aacgttcgat gaacactggc tatgctggtg tgcaaaaccc gctgttcttc aaggaaaaca
    23701 cccacatgct gtttggtgac gccaaagcca gcgtggatgc aatcctgaaa gctctgtaac
    23761 cctgacggcc tctgcggagg ccgtcactct ttattgagat cgcttaacag aacggcgatg
    23821 ctttgacctc ccgctgtttg ttcaagcgca attttgacaa taattgtcaa cggcacggaa
    23881 agcagcatac ccaccggtcc taacaaccat ccccaaaaaa tcaacgacaa aaataccacc
    23941 aatgtcgaaa gccccagccc acgtcccatg atgcgcggct ccagaatatt gccgaagacc
    24001 agattaatca gcagatatcc cgccagcacc agcaacgctt cgtagaagcc attaaacacc
    24061 agtacctgag cgataggggg gattgccgcg agaactgaac caatattagg gatgtaatta
    24121 agcgcaaagg ccagcaatcc ccagacaaaa gcgaagcgaa catcgagtgc ggcgagcatc
    24181 gcccaggcga ccaggccggt gatgatgctg atggctgttt tcagcaccag ataatgagaa
    24241 acactgtcaa tcgcacgttg aatcgccgcc atcccttcaa ccggacgcgc catcatttgc
    24301 tgaaattttc cgggcaattg tggcacttcg agcagcataa acagcaccgt cagcagcaat
    24361 aaaaatattg atgacatggc attagataac tgcgtcaata agttggtgag caacgtcatc
    24421 gccgcgttcg gatcaatgta atgcgccagt tggtcaactg agacgtcaat ccctacgcgt
    24481 tgcaacaacg gctcaagagc ttgcagcggc gtcataatag agttgcgata ttgcggtaac
    24541 gtccacgtca actcgttgag cgcggaaccc agataagcta atagcaacac catcgccatc
    24601 acgatgattg tcatcaaaat cgacactgcc agtacacgtg gcacacgcca gcggaccata
    24661 tgttgcacca gcgggttaag aataacagca ataaataatg cgagaataaa cggcacgatg
    24721 atctcggcgg caaaacggat accgcagaga ataatgacca gcattcccaa cataatgacg
    24781 atttttaggc cattgagcgt gatgatcggc tttgccatgc ttgtccccgt ttttcttcct
    24841 ttgctaataa taatgtcttt ttcgctccgt catcataacg aaaatcaaac cgctcaggta
    24901 aagaagtgaa aatattttga gttaattctt aagctatgat acaaatcagg cgtgttcaac
    24961 taccgaggac aattatcatc tgcgatgacg agaagcaaca ctgcggataa ttgtaatatt
    25021 atggacaatt tgttcaggac gttattttct acatttaccc atcttcgtac ctcatccact
    25081 atcttgctcg taggtgagca gcactggcgc aacgcgcttt agtcctttct tctgcctaag
    25141 cttgccctat gcgcagggct tgaccacttt actttggttt cgctgaatta agcgaaaatt
    25201 aaaatagttc tcttgcagga gaaggacaat gtatatttat tggattttat taggtctggc
    25261 tattgctaca gaaattaccg gtacgctgtc aatgaaatgg gcgagcgtca gtgagggaaa
    25321 tggcggcttt attttaatgc tggtgatgat ttctctgtcg tatatatttc tctctttcgc
    25381 cgttaaaaaa atcgccttag gcgtagctta tgcgctgtgg gaaggtatcg gtattttatt
    25441 tattaccttg tttagcgttt tgttattcga cgaaagttta tcgctgatga aaattgccgg
    25501 gttaaccacc ctggtcgccg ggatagtgtt gatcaaatca ggtacccgta aagcgcgtaa
    25561 acctgaactg gaggtgaacc atggcgcagt ttgaatgggt tcacgccgcc tggctggcat
    25621 tggcaatcgt gctggaaatc gttgctaacg tctttttgaa attttctgac ggctttcgtc
    25681 gcaaaatatt tggcttgctc tccctggcgg cggtgctggc ggcctttagt gcgctttctc
    25741 aggccgttaa agggatcgac ttgtctgtcg tttatgcatt gtggggcggg tttggtattg
    25801 ccgccacgtt agccgcaggt tggatcttgt ttggtcaacg gttaaatcgt aaaggctgga
    25861 ttggcctggt cttgctgttg gctggaatga tcatggtgaa acttgcctga tgaagacgct
    25921 gcccgcgctg tcgggcagcg tttgaacatt atttttgcga cagttgatcc agcttgtcgc
    25981 gaaaaccggt aacagaaatg gcccggttat cggcgcgcca gcgatctttc gcggcaggag
    26041 ccgaactttg caccccaatt aattgccagc cgtcatcggt atgcaacatc agaggcgaac
    26101 cgctgtcacc cggcaaggta tcgcactgat gtgacatcac cgacgtttgc gcccagccag
    26161 tcacttcaca gttttgatga ctgtataacg tatcgagatg atcttcaggg tatcccgcct
    26221 gagtcacttt acgaccagcc gattttaatg cggcagtaag cgcggcttta tctccctcaa
    26281 ataacggcaa cggtgtaatg ccagaagggg gattacgtag cacaatcaat ccgaagtccc
    26341 acggtgcagc tgcgggagga acaatccaac catccccatc tgcttttaac cgctttccca
    26401 gtgtcggatc aacgcggcct tctatgtcgt ggatctcata gcgccaaaga cctttatttg
    26461 acacaaaacg cagcgctacc gccttatccg ctttaccctt aggcggcgtc aataaacagt
    26521 gtcctgccgt taatgccaga ttgggtgcaa tcagcgtcgc cgtacataaa ttgccgctgg
    26581 ccgtttccag ttgcccaacc gcatcccacg gtgattgggt cgtgtcattc actggcacac
    26641 gatcatcatg accaaaaaac agggtgctga cctcatcgtt tgccgatttg gcaacgtctg
    26701 gtttatctgc aaacacaaaa gcagacgtca aactaattgc acccaacact acagcaatgg
    26761 ttgtacgcat atcacactct ggtgggtaat tatgattatt aaaagcaaac cctcataaat
    26821 actatagacg ggacgggatg aaagtgggag taaaatcaga taactacagt caggaaagat
    26881 aaaaacgggc ggcaatcagt gcgcataaaa taaggatgat aagaatgaac tcaaagcgat
    26941 aacggcccac catactaccc tccgctaaag gcggtgctaa acgttacccg cttagcaccg
    27001 taattttcag gcgcgagggg gcgcgccagc attactgttg aaaacttacg ctgcgggttg
    27061 tgcagcaggt tttgccggtt gctgatggcc gtgtttcccg gcgtgtttct tcgctgcctg
    27121 cgctttttgt tcaggggctt tctgttcagc tttcgtattt ttatgatgct ttttagccgc
    27181 ctgcgctttc tgggcaggtg ctgctttatg ctgtttttta tgatgtgtag ttttcgctgg
    27241 cgctgctttg gtggtcgtcg cagtcggagc aggtgtggtc gcagtctctg cagcaaaggc
    27301 ggcagaagac agacccatag cagcggcaac aaccagagct aatacttttt tcattgtcat
    27361 accctcaatt tgttttttca tttaacccca ctgcggggcc gttgaaataa ctatatccct
    27421 gggaatggca gacttccgtg agtggttggt ttcagcgtgt aacgatatgt acaaacgctg
    27481 aataaattac agcgttgata ataacgtatt gtgacttaag gaaaatttag ctaccaataa
    27541 tagtagtctt gatcgggtta actattatac tacttacaaa gcggtagaat aactgcgcat
    27601 cagtaataaa tgacacacag caaaatgaat ccgtttattt gggtacttat aatcctgatg
    27661 acgctagatg cgctgcggga attggctggc gcttcgtcta ttttaggatg gctattaacg
    27721 ttggtttgag caggcaataa gtccggacgg gtatttaccg cagtccggac ttatttttca
    27781 ggcgtgcaga cgacgatgca aacgcgtccc gaccagcaga gcaatgacca gcatcagagc
    27841 aataaatgcg ccgacgccgt tccagccata gttatgccag aaaacaccac ccagcgtccc
    27901 ggcaatactc gaccccagat agtaactgaa cagatacagc gaggaggcct agcctttagc
    27961 gcgttttgcg cgggggccga tccagctgct ggctactgag tgggctgcga agaatccggc
    28021 tgagaagagt aacattccgg caaagatcag ccacagcgag ctaaataagg tcatcagtaa
    28081 accaaacggc ataactcccg tcgaaaacaa catcaccgga ccgcgcccat agcgggtggt
    28141 catggttccg gctttgggtg agctccatgt accggtcaaa taagccagcg ataataagcc
    28201 aaccacggcc tgactgacat gctaggggga aagcatcaac cgatagccga tgtaattaaa
    28261 cagcgtgacg aacgacccca tcagcaaaaa gccttctgcg aacaataacg gtaatccccg
    28321 gtcacgccag tgcagacgaa agttgataaa taacgtctta gggcgcagcg aagtcgggca
    28381 aaaatggcgt gattcaggga ggattttcca gaacatcaac gccgaggcca gcgcgaaaca
    28441 accgattgcc gccagagcaa ttcgccagtt gaaaaagtcc gtgaagacac cactaattaa
    28501 gcgtccgctc atgccgccaa ttgagttgcc gctgatatac aaccccatcg aaaaggccac
    28561 gaaactgggg tggatttcct cgctaagata agtcatgcca acagctgcca cgccacttaa
    28621 cgaaagccca atcaaggcgc gcataatcaa aatgccgtgc cagctggtca tcattgtcga
    28681 aagtaacgta caaatggagg ccaacagtag cgccgtgacc atcactggtt tgcgaccaat
    28741 ggcatcggat agcgggccag taaatagcaa accgatagcc aacatcgccg tggaaatgga
    28801 cagtgaaata ctactgttcg cgggcgttaa gccaaactcc tgcgaaagca ccggaaggat
    28861 aggctgcaca caatagagaa gtgcaaaggt tgccagtccg gcagagaaca gcgccagggt
    28921 gacgcgcata aattgcggcg taccgcgttt aataaattga tttggctgag aaatgctttg
    28981 cttgtcagtg tcgcttgccg gagcgccatc aacagttgta gtacggctca cttgaaatcc
    29041 ttgctaaata tgcctgtgga tcaggcttat acatagggta ggaaaatcga attgttctgt
    29101 ctaatatatt aataatctca aataagatgt tttaaatatg aatattgaac ttcgtcatct
    29161 gcgttacttt gttgctgttg cggaagagct gcattttggg cgcgcggctg cccgcctgaa
    29221 tatttcgcaa ccgccgctaa gtcagcagat tcaggcactg gagcaacaaa tcggtgcccg
    29281 actgctggca cgaactaatc gcagtgtatt gctgacggca gcaggaaaac agtttcttgc
    29341 agatagtcgg caaatcctgt ctatggtgga tgacgctgcc gctcgcgctg aaagactgca
    29401 tcagggtgaa gcgggggagt tgcgtattgg ttttacttcg tccgctcctt ttattcgggc
    29461 ggtgtccgat acgttatcgc tgtttcgccg tgattatcct gatgtccatt tacaaacccg
    29521 cgaaatgaac actcgcgagc aaatcgctcc gctcattgaa ggaacgctgg atatgggatt
    29581 gctgcgtaac acagcgttac cggagacgct tgaacacgca gtcatcgtcc atgaaccgct
    29641 tatggcgatg atcccgtacg atcatcccct ggcaaataat ccgagtgtaa cactggctga
    29701 actggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg ggactgggct
    29761 gtatgacgat attctcggac tgatgcgacg ttaccatttg acgcccgtca tcactcagga
    29821 ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg tttcaatttt
    29881 gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga ttgctgaaga
    29941 ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa gtccagctgc
    30001 gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc agcgaaaaag
    30061 cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac gtattgaagt
    30121 gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta tgcggtggtt
    30181 gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg cgcggtttat
    30241 cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct ggcgcagctg
    30301 gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct ggtgcgagag
    30361 ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt ggttgaaact
    30421 gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct tgctctgcgc
    30481 gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa agatgacttg
    30541 ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca ccagaaaaaa
    30601 cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac ggaaaatggt
    30661 attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg cgaggcgctg
    30721 gagcagcata ccggcgtacc ggtttatatt cagcatgata tcagcgcatg gacgatggca
    30781 gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt tatcgatcac
    30841 aacgtggggg tgggcgtcat taccgatggt catctgctac acgcaggcag cagtagtctc
    30901 gtggaaatag gccacacaca ggtcgaccca tatggtaaac gctgttattg cgggaaccac
    30961 ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca gctgcgtctc
    31021 aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc attgtgtcag
    31081 gcggcattgc gcggcgatct actggcaaaa gacatcatta tcggggtggg cgcgcatgta
    31141 gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact gattggctca
    31201 ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagacagcat ccgtcagcag
    31261 gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc taaccagggc
    31321 acgatggcag gcgctgcact ggtaaaagac gcgatgtata acggttcttt gttgattcgt
    31381 ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc tcaatttggg
    31441 ccaggaaagc ataacttaga ctttcaaggt taattatttt cctagtttat atttgtgaag
    31501 cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag acacttctgt
    31561 agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg gaaaaagggt
    31621 tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc tgcgtaataa
    31681 agatgccttg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag cagttaatcc
    31741 tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca attacaccct
    31801 catttcaaac ggcctggcaa acctgaccga taaagtcgat catgtcgtgg tagaagggac
    31861 tggcggctgg cgcagtctga tgaatgattt gcgtccactc tctgaatggg tagtgcagga
    31921 acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc atgcactgct
    31981 aacagctcag gcgatcgcca acgacgggct gccgctcatt ggctgggtgg ctaaccgaat
    32041 caacccagga ctggcgcatt atgcggaaat cattgatgtg ctgggtaaaa aacttccggc
    32101 accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac tggggcaata
    32161 catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg tgtaacgtcc
    32221 gcgaaattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc tgatactccc
    32281 cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt gccgccaaca
    32341 gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca gggaaccata
    32401 atcccaggct acgaccatac aacatgccaa tggcaagacc gataaatagc gtcggtgtaa
    32461 agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt ttacaaagga
    32521 agatcccggc aatgatcatt aacagtggtg gggcggttaa aaaggattgt acggtgctat
    32581 agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc aacccgacta
    32641 gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga caggcgttca
    32701 ttaacgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg ataatcagcg
    32761 catagtcacg agcctgaacc gtcactgaga gttgtacgtt gtagagtaac gcgtcgctat
    32821 gattaatcag attgctaacc agccatgcca cgatggcgga aataatcacc gggccgagag
    32881 aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca gcaagcgggg
    32941 cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat ttccattcct
    33001 ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa agaatcatcg
    33061 caccttcgcg accaattgca ctgccgctgg ttactaccag cagcgaggca agcgatttaa
    33121 ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg taatcggtcg
    33181 gcgcatgagg gcgttgttgg gtaaatttct gccagcccat cagcaacaaa cccgccgcca
    33241 gtccgccgag cgccggagtt agcagccgtc gccaggagga aaggtttgtc gctgcattga
    33301 ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc gcatgacgaa
    33361 acccggcaac ggcaaagaca gcgagaatac cgacgactgt tgcgataagc agacggcgga
    33421 acatagtgcg cagatccgga tagatgtgta gatgatgcat gagcagaaat gggcaatgaa
    33481 acacgggcga ctattttgcg gcaaaagagg ccaaccgcaa aagcagtgcg tgctttaaga
    33541 gataaaaaaa cgtgacattg taacgtatta ttgccgaatc gcgcgtaatc gtcaccatcc
    33601 ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa tcaacagttg
    33661 tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt agaaagggtg
    33721 ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa ggtgccatgc
    33781 cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag ccatcaacag
    33841 cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa tgcctttctc
    33901 gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt cgcgatccag
    33961 ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca aacgctgcca
    34021 ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg gcgctaatga
    34081 cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg caaccagagg
    34141 tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc gcgccgcgac
    34201 agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat gtgcgggttg
    34261 ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca ggccataaaa
    34321 gagcacacgc ccgatcatct caccaacaag tatgaggatt agccccagaa caagtccggc
    34381 aacgtgaggt tcacggcgac gaatgagcgg acaaagccag catccaagac ctgcgcataa
    34441 caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc tggcttgttg
    34501 cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcacct cggcacaagc
    34561 gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa cgcgggctgc
    34621 ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga agaaagccag
    34681 cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt tgatacacgc aggtcatcat
    34741 ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc ccaacgcttg
    34801 cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa aaaatatgga
    34861 accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg agttaaacgc
    34921 tcgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca tcaacagcca
    34981 gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcatttttgg cggcgaacca
    35041 gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg taaagataac
    35101 cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta gccagataac
    35161 cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata ttgggttttg
    35221 tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc tggcgcagct
    35281 cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacgcat atgggttgtt
    35341 tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct ttttcagcat
    35401 tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta gcggcagccg atacaaacat
    35461 cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg gggcagactt
    35521 ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa gcgaacacgt
    35581 tctggtgcca gatgccatta tcttcctgcc agtcgccgcc agcatattca taaatacggc
    35641 ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg caggttttac
    35701 agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg ggctactcct
    35761 taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc cagcggtgaa
    35821 gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc accaaacata
    35881 tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat acgcggagtc
    35941 acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt atcgccatga
    36001 cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg gcaggcctgc
    36061 tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttggtttg gcgacttcac
    36121 tcagcatcca gtagatgggt ttgcgttcaa attttgctga ggttgcgggt tgggcgagga
    36181 tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc tctgttggca
    36241 tcaggtcagg caacaggata tcgcaatact tagccgaggc cgtcatgaag tggtcaatgc
    36301 caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga ttgatgtcgc
    36361 catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg gggacatcca
    36421 gtttttcttt tccacgaaca ccatcacggg tcgcggtcat tttcgtacca tggtcgatgg
    36481 catctgtcca tgtaaaaacg gaaatctgcg ttttaacagg attctcgagc atcgggaacc
    36541 attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg tttatgccga
    36601 cgttaccggt aagaacggaa agcatggcaa tagcgcggga cgtttgttcg ccgttggagt
    36661 gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg atctctcgtg
    36721 ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat tccggcgttt
    36781 tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg ttacgtggtg
    36841 cgttggcggg cagcgttttt tcatcgtaac caacacaata tttgtcgaga aatggctgat
    36901 cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg ccatcggtgc
    36961 cagggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta tagcgtggat
    37021 cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag taagtgacac
    37081 caccgccgct catccgcgtt tctgccgggt gatttccgaa cataacgacc agtttcgtat
    37141 tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc attgcggcac
    37201 tgatctgtgc ggtactgtag ctgccatagc gactgagaaa acccccgcaa gagttcatca
    37261 gacggtacgg gacgttcgag ttggtgatat ttccgccatc tacgcctgtt ccgtacagga
    37321 catgtacagc ctcattgccg taatctttca ggatccgccg aagattatca ctgatggtat
    37381 ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgctcg ccgacgcgct
    37441 tcatgggata tttcaacctg tcaggatgat tcatccgtcg gcggatagag cgcccgcgta
    37501 aacatgcccg aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca gactccaccc
    37561 agtacacggt gtcatctttc acatgcaaac gtagcagaca gcggctcccg cagttaacgg
    37621 tgcaggaact ccagaccgtt ttctcttcta ccggagcctc tgccgcccgg accatttggg
    37681 aaaatggcag agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg gaggttttca
    37741 tcagacttct atggctgatt tcagccttca tgagcgcctc tgtggtatgg attttcatca
    37801 ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt gtagtgggtt
    37861 atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc agtggtgaag
    37921 ggcgcagagt ggttagcgta ttcacacatc cgccatggtc gattttatcg ccagacatat
    37981 tgggctcgtg ccaggctccc tggcccatag cgctaactcc agggagaatg cgtggtgtta
    38041 ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata tcgccgttgg
    38101 caatcccacg tttctgtgca tctatagggt tgatccacac ctcctgacgg caggctgcct
    38161 tcaggacatc aatgttgccg taggtcgagt gagtacggga tttgtaatgg aaaccaaaca
    38221 gttgcagtgg gaaggttcta cgttcagggg agtcccagcc ttcaaaggtt gaggcataaa
    38281 ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca atttccgcca
    38341 gcttgctgga ataaatttca atcttaccgg aaggcgtttt aagtggattt gcctcggggt
    38401 cgtcacgaaa tgctttgtag gcgacaaaat ggccattggg atctttacgc ttatagatac
    38461 ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc attttggcgt
    38521 acagatgttg taaccattgt tcctgcgtgc gaccttctgt gaacttttga tagacgtcag
    38581 gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt tcgaattttt
    38641 cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag tcgttaggaa
    38701 taatatcttc ctgctcaacg gtcatcaggt ctgccagcag aatgtcggca tattttgccg
    38761 atgaggtcat aaagttttcg atgaccacaa tcatttcgca tttcgattcg tcctgcagaa
    38821 tttcatgcgt tttgttgatg tcagaatgct gattaacgag ggtatttccc gcgtagttcc
    38881 agatggactc tactgtcaga cggatggacg gacttgaagt agttgactca acgctactta
    38941 aggtcctgat atgactgaca ttaaccatga cgatactgca tatccatcca gttctttctc
    39001 tgggtctccc tatctgtatc agcaagtcta cacctcaagt cctgacacaa ggctgacaca
    39061 aatcaaggac cttgctacgc cacagctagt ccggagaacc aacgggcgtt acactgtccg
    39121 cttcagactt aaaggagcta ccagcccgtt tctatcagtc tcaacccgca gcacagaccg
    39181 gaggaccgct acaatgcgtc agagagagct cgcagccaca gctaaggcat tcctactgga
    39241 caatcctgaa gtcacactac aggagcttac agcgcacata cggtccatgg ctgagcagtt
    39301 cctgactgat gccagtgatg actactggaa tggcgtagag gtggcgacac tggttgacga
    39361 gaagtccaac ctcagggaac ttgcagcgac aagagcgttg agtgtggacc agcagagggg
    39421 cattaagtta gcgctggaag tcctgaccgc agcacagcag agagtcgaca ctggtgatac
    39481 ctctggtctg attaagctga ttgatggtgg cgtaagccag cttactgatg ataataacca
    39541 tactgattac tcaactatag gcgactctac gtcgattctg aagaatgagc agggggatag
    39601 accagcagtt ttcacacagg aacgtcagtt actcactcca gagtcacgag aatcatctgt
    39661 actttttcag gaattggtga catcactgct ggctgagaag gtccagacac tgaaacaatc
    39721 cagctttaag gacttgtcag cgtccctgtg aaccgccccg ggtttcctgg agagtgtttt
    39781 atctgtgaac tcaggctgcc agatcatcgt ttccgatgga agcataataa gcttttctgc
    39841 ttctgccgga ggagt
//
LOCUS       DAECNO010000029        38530 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.029, whole genome shotgun sequence.
ACCESSION   DAECNO010000029 DAECNO010000000
VERSION     DAECNO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.029"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..73)
                     /locus_tag="KKO08_001533"
     CDS             complement(<1..73)
                     /locus_tag="KKO08_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HBD6317830.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="KKO08_001534"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="KKO08_001534"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBD6317831.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="KKO08_001535"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="KKO08_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBD6317832.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="KKO08_001536"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="KKO08_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBD6317833.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="KKO08_001537"
     CDS             2703..2939
                     /locus_tag="KKO08_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBD6317834.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="KKO08_001538"
     CDS             2942..3799
                     /locus_tag="KKO08_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6317835.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5796
                     /locus_tag="KKO08_001539"
                     /pseudo
     CDS             3799..5796
                     /locus_tag="KKO08_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="KKO08_001540"
     CDS             complement(5797..6318)
                     /gene="sodC"
                     /locus_tag="KKO08_001540"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBD6317836.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6399..7295)
                     /locus_tag="KKO08_001541"
     CDS             complement(6399..7295)
                     /locus_tag="KKO08_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBD6317837.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7344..7583)
                     /locus_tag="KKO08_001542"
     CDS             complement(7344..7583)
                     /locus_tag="KKO08_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBD6317838.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7686..8285
                     /gene="nemR"
                     /locus_tag="KKO08_001543"
     CDS             7686..8285
                     /gene="nemR"
                     /locus_tag="KKO08_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBD6317839.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8322..9419
                     /gene="nemA"
                     /locus_tag="KKO08_001544"
     CDS             8322..9419
                     /gene="nemA"
                     /locus_tag="KKO08_001544"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBD6317840.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9500..9907
                     /gene="gloA"
                     /locus_tag="KKO08_001545"
     CDS             9500..9907
                     /gene="gloA"
                     /locus_tag="KKO08_001545"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBD6317841.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10010..10657
                     /gene="rnt"
                     /locus_tag="KKO08_001546"
     CDS             10010..10657
                     /gene="rnt"
                     /locus_tag="KKO08_001546"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBD6317842.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10750..15208
                     /locus_tag="KKO08_001547"
                     /pseudo
     CDS             10750..15208
                     /locus_tag="KKO08_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15252..16460)
                     /locus_tag="KKO08_001548"
     CDS             complement(15252..16460)
                     /locus_tag="KKO08_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6317843.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16468..16968
                     /gene="tnpA"
                     /locus_tag="KKO08_001549"
     CDS             16468..16968
                     /gene="tnpA"
                     /locus_tag="KKO08_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBD6317844.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     THRIKD"
     gene            complement(17007..17354)
                     /gene="grxD"
                     /locus_tag="KKO08_001550"
     CDS             complement(17007..17354)
                     /gene="grxD"
                     /locus_tag="KKO08_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBD6317845.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17689..18516
                     /gene="mepH"
                     /locus_tag="KKO08_001551"
     CDS             17689..18516
                     /gene="mepH"
                     /locus_tag="KKO08_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBD6317846.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18644..19225
                     /gene="sodB"
                     /locus_tag="KKO08_001552"
     CDS             18644..19225
                     /gene="sodB"
                     /locus_tag="KKO08_001552"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBD6317847.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19371..20540)
                     /locus_tag="KKO08_001553"
     CDS             complement(19371..20540)
                     /locus_tag="KKO08_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6317848.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20706..20795)
                     /gene="ynhF"
                     /locus_tag="KKO08_001554"
     CDS             complement(20706..20795)
                     /gene="ynhF"
                     /locus_tag="KKO08_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBD6317849.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            21094..22119
                     /gene="purR"
                     /locus_tag="KKO08_001555"
     CDS             21094..22119
                     /gene="purR"
                     /locus_tag="KKO08_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBD6317850.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(22116..23048)
                     /locus_tag="KKO08_001556"
     CDS             complement(22116..23048)
                     /locus_tag="KKO08_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6317851.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            23161..24372
                     /locus_tag="KKO08_001557"
     CDS             23161..24372
                     /locus_tag="KKO08_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBD6317852.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24663..25811
                     /gene="cfa"
                     /locus_tag="KKO08_001558"
     CDS             24663..25811
                     /gene="cfa"
                     /locus_tag="KKO08_001558"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBD6317853.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25851..26492)
                     /gene="ribE"
                     /locus_tag="KKO08_001559"
     CDS             complement(25851..26492)
                     /gene="ribE"
                     /locus_tag="KKO08_001559"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBD6317854.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26707..28080
                     /gene="mdtK"
                     /locus_tag="KKO08_001560"
     CDS             26707..28080
                     /gene="mdtK"
                     /locus_tag="KKO08_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBD6317855.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(28121..29377)
                     /locus_tag="KKO08_001561"
     CDS             complement(28121..29377)
                     /locus_tag="KKO08_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBD6317856.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29685..29761
                     /locus_tag="KKO08_001562"
     tRNA            29685..29761
                     /locus_tag="KKO08_001562"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29719..29721,aa:Val,seq:gac)
     gene            29766..29842
                     /locus_tag="KKO08_001563"
     tRNA            29766..29842
                     /locus_tag="KKO08_001563"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29800..29802,aa:Val,seq:gac)
     gene            29950..30255
                     /locus_tag="KKO08_001564"
     CDS             29950..30255
                     /locus_tag="KKO08_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBD6317857.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30381..31985
                     /locus_tag="KKO08_001565"
     CDS             30381..31985
                     /locus_tag="KKO08_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBD6317858.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(31997..32809)
                     /locus_tag="KKO08_001566"
     CDS             complement(31997..32809)
                     /locus_tag="KKO08_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317859.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32813..33598)
                     /locus_tag="KKO08_001567"
     CDS             complement(32813..33598)
                     /locus_tag="KKO08_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBD6317860.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33595..34263)
                     /locus_tag="KKO08_001568"
     CDS             complement(33595..34263)
                     /locus_tag="KKO08_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBD6317861.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34327..34965)
                     /locus_tag="KKO08_001569"
     CDS             complement(34327..34965)
                     /locus_tag="KKO08_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBD6317862.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34978..37079)
                     /locus_tag="KKO08_001570"
                     /pseudo
     CDS             complement(34978..37079)
                     /locus_tag="KKO08_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(37100..37726)
                     /locus_tag="KKO08_001571"
     CDS             complement(37100..37726)
                     /locus_tag="KKO08_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBD6317863.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(38182..38391)
                     /gene="fumD"
                     /locus_tag="KKO08_001572"
     CDS             complement(38182..38391)
                     /gene="fumD"
                     /locus_tag="KKO08_001572"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBD6317864.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9279 a        10056 c         9433 g         9762 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccggctg cccaggaatg agcagtgaca gattggcgat gccgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gcaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tacttttggt caaatttggc ctgatggtcc agattagcga tctgtggcaa
     5101 tttttactgt ttctctttcc actgctggcg acaatgcagc ttcttaaatt gcagatgccc
     5161 aaatttgccg cactgtgggg gcaactgatt gtttttatgg gttcgtttat cgctgtcact
     5221 aatcccccgg tgtatgattt tgctgatttt cttaactata atctggcaaa aatcgttggc
     5281 gttgcgctgg cttggctagc gttcgccatt ctgcgtccag gatcggatgc tcgtaaaagc
     5341 cgccgccata ttcgcgcgct gcgccgggat tttgtcgatc agctaagccg ccatccaaca
     5401 ctgagtgaaa gcgaatttga atcgctcact tatcatcacg tcagtcagtt gagtaacagc
     5461 caggatgcgc tggctcgccg ttggttatta cgctggggtg tagtgctgct gaactgttct
     5521 catgttgtct ggcaattgcg cgactgggaa tcgcgttccg atccgttatc gcgagtacgg
     5581 gataactgta tttcactgtt gcggggagtg atgagtgagc gtggcgttca gcaaaaatca
     5641 ctggcggcca cacttgaaga attacagcgg atttgcgaca gccttgcccg tcatcatcaa
     5701 cctgccgccc gtgagctggc ggcaattgtc tggcggctgt actgctcgct ttcgcaactt
     5761 gagcaagcac caccgcaagg tacgctggcc tcgtaattac ttaattacac cacaggcata
     5821 gcgttcaccg ccaccgccca gcggtttagg ttgatcggac atattatcgc cgccaacgtg
     5881 gaccatcagc gccttgtctt tgatttcatc cagtgatttc agacgaggcg cgatgacggc
     5941 atcggtagct ttgccgtcat tattgacgac cagtgcaggc agatcgccta aatgcccggt
     6001 accttctggc ccttcatgtt taccggtatt ttgtggatca agatgcccgc ctgcggattc
     6061 cgcggcgctg gctttgccat ctttggtggc tggctggcag cttcctttgg catgaatatg
     6121 gaagccatgt tcaccggggg gtaatgcttt cagatcgggt gaaaactcca gacctttatc
     6181 ggtttcagta atggtgacgc taccaattga ctgcccaacc ccttgcgacg tgacgaggtt
     6241 catctcgact ttttcactgg cagcttgtgc gccggtcgca acaaccagcg ccagaatggc
     6301 cagactaaaa cgtttcatag gacctccgtt cattgtgcat tcctgaataa gtgtaaacca
     6361 gtgacacatt tttgaacggc ggctattcct aaaagtgctt acggtacgtc gtaccccagt
     6421 gcagctttac ggatacgaaa ccattgttga cgggtcattt tcagtgtttc tgcttcgaca
     6481 gctgcccgta cgcgctcaat tttacctgag ccgataattg gcagcggctg cgacggtaaa
     6541 cgtaataccc aggcgtaaac cacctgttca atcgagcccg cgtttaactc ctccgccacc
     6601 acagccagtt catcacgcag cggctggaaa taatcatcat taaacagacg accaccacca
     6661 aggcaggacc acgccatcgg acgaacacgc agttgttgta gttggtcgag cgtgccatcc
     6721 agcagtaacg gctgatgcac cggggatatt tccacctgat tagtggcaag ggtaaacggc
     6781 agacgtgatt gcaacagggc aaattgcgca ggcgtaaagt tcgatacgcc aaaatgacgc
     6841 actttgccgc tctgatgcag atgtttgaac gcgtccgcca cttcatcggc atccattaac
     6901 gggtctggtc ggtggattaa cagcagatcc agatgatcgg tcgcgagatt aattagcgac
     6961 tgttcggcgc tcttaatgat gtgattgcgg tcagtgatgt aatgaccaat gacgttttct
     7021 tcacgcgcgg tcgtcgcgat accgcattta ctgacgattt ccatccgttc acgcaggtga
     7081 ggtgccagtt tcagtgcctc gccaaacgcc gcttcgcact gatagccacc ataaatatca
     7141 gcatggtcca cggtggtcac gccgagatcc agatgctctt caataaaact gaccagctgg
     7201 cgggcggaca tattccagtc catcaatcgc cagtagccca tcacaaaacg ggaaaactcc
     7261 gggccttccg gcgcaatagt aatacgctga accataatcg cttcctctta tcagatataa
     7321 gaggagtata cgcaagatta ggttcaaaag agtgatggtt gctccggttc gtctgatgac
     7381 gctggcttat ttgcgcgtaa tttgcgcatt aatcgctgcc gacaaaggcg cagcacctct
     7441 tgtttttcgc catcgctcat tttattccag ttaaaacgct catcccgact acgaaaacag
     7501 ccgcgacaaa acccgcgttc gtcagactgg caaattcccc ggcacgggct ctggacggga
     7561 aagaactcta attgctccgc cacctcgccc tcctcagata agattattac cattattgaa
     7621 gctgttaatg tccaaagtag caactttgct tgcactagac cgactggtct actacactcc
     7681 aacgcatgaa caaacacacc gaacatgata ctcgcgaaca tctcttggcg acgggcgagc
     7741 aactttgcct gcaacgtgga ttcaccggga tggggctaag cgaattacta aaaaccgctg
     7801 aagtgccgaa agggtccttc tatcactact ttcgctctaa agaagcgttt ggcgttgcca
     7861 tgcttgagcg tcattacgcc gcatatcacc agcgactggc tgagttgctg caatccggcg
     7921 aaggtaacta ccgcgaccgc atactggctt attaccagca aacactgaac cagttttgcc
     7981 aacatggaac catcagtggt tgcctgacag taaaactctc tgccgaagtg tgcgatctgt
     8041 cagaagatat gcgcagcgcg atggataaag gtgctcgcgg cgtgatcgcc ctgctctcgc
     8101 aggctctgga aaatggccgt gagaaccatt gtttaacctt ttgtggcgaa ccgctacaac
     8161 aggcacaagt gctttacgca ctatggcttg gcgcgaatct gcaggccaaa atttcgcgca
     8221 gtttcgagcc actggaaaac gcgctggccc atgtaaaaaa cattattgcg acgcctgccg
     8281 tttagcaggc attttttatc accagacgac cgggagcctt tatgtcatct gaaaaactgt
     8341 attccccact gaaagtgggc gcgatcacgg cggcaaaccg tatttttatg gcaccgctga
     8401 cgcgtctgcg cagtattgaa ccgggtgaca ttcctacccc gttgatggcg gaatactatc
     8461 gccaacgtgc cagtgccggt ttgattatta gtgaagccac acaaatttct gcccaggcaa
     8521 aaggatatgc aggtgcgcct ggcatccata gtccggagca aattgccgca tggaaaaaaa
     8581 tcaccgctgg cgttcatgct gaaaatggtc atatggcctt gcagctgtgg cacaccggac
     8641 gcatttctca cgccagcctg caacctggcg gtcaggcacc ggtagcgcct tcagcactta
     8701 gcgcgggaac acgtacttct ctgcgcgatg aaaatggtca ggcgatccgc gttgaaacat
     8761 ccatgccgcg tgcgcttgaa ctggaagaga ttccgggtat cgtcaatgat ttccgtcagg
     8821 ccattgctaa cgcgcgtgaa gccgcttttg atctggtaga gctccactct gctcacggtt
     8881 atttgctgca tcagttcctt tccccttctt caaaccatcg taccgatcag tacggcggca
     8941 gcgtggaaaa tcgcgcacgt ttggtactgg aagtggtcga tgccgggatt gaagaatggg
     9001 gtgccgatcg cattggcatt cgcgtttcgc caatcggtac tttccagaac acagataacg
     9061 gcccgaatga agaagccgat gcactgtatc tgattgaaca actgggtaaa cgcggcattg
     9121 cttatctgca tatgtcagaa ccagattggg cggggggtga accgtatact gatgcgttcc
     9181 gcgaaaaagt acgcgcccgt ttccacggtc cgattatcgg cgcaggtgca tacacagtag
     9241 aaaaagctga aacgctgatc ggcaaagggt taattgatgc ggtggcattt ggtcgtgact
     9301 ggattgcgaa cccggatctg gtcgcccgct tgcagctcaa agctgagctt aacccacagc
     9361 gtgccgaaag tttctacggt ggcggcgcgg aaggctatac cgattacccg acgttgtaat
     9421 ccaacattgc gagcggcgta aagccgccgc tatactaaaa caacattttg aatctgttag
     9481 ccattttgag gataaaaaga tgcgtcttct tcataccatg ctgcgcgttg gcgatttgca
     9541 acgctccatc gatttttata ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa
     9601 cccggaatac aaatactcac tggcgtttgt tggctacggc ccggaaaccg aagaagcggt
     9661 gattgaactg acctacaact ggggcgtgga taaatacgaa ctcggcactg cttatggtca
     9721 catcgcgctt agcgtagata acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg
     9781 taacgtgacc cgtgaagcgg gtccggtaaa aggcggtact acggttatcg cgtttgtgga
     9841 agatccggac ggttacaaaa ttgagttaat cgaagagaaa gacgccggtc gcggtctggg
     9901 caactaatct cctgccgggc gtgaactcat cgcgcccgca tctttactgc atcgacaagt
     9961 aatatttgtc ataatgcgcg ctgcaattta tccgtattaa gagaatcaga tgtccgataa
    10021 cgctcaactt accggtctgt gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt
    10081 tgaaacagcc ggatttaacg ccaaaaccga tgcgctgctt gagattgccg ccatcaccct
    10141 gaaaatggat gaacaaggct ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc
    10201 atttgtcggc gcaaatttgc aaccagaagc cctcgccttc aacggcattg acccgaacga
    10261 tcccgatcgc ggcgcggtca gcgaatacga ggcgctgcac gaaattttta aagttgtacg
    10321 taaaggtatt aaagcgagcg gctgtaaccg cgccattatg gtggcgcaca acgccaattt
    10381 tgatcacagc tttatgatgg ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca
    10441 ccctttcgcc acttttgaca ctgctgcact ggccgggctg gcactcggac aaaccgtatt
    10501 gtcaaaggct tgccagaccg ctggcatgga cttcgacagc acccaggcgc actccgcgct
    10561 gtacgacacc gaacgcactg ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct
    10621 gggaggctgg ccgctacctg ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg
    10681 caatgattca ggcatctata gtgaggctat tccacgcatc aagcatgata ttcacgggga
    10741 atagcgttaa tggcagataa tccagaccct tcatcgctcc tgccggacgt gttttcaccg
    10801 gcgacccgcg actggtttct tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc
    10861 tggcatgtgg cggcgcgagg cgaacatgcg ctggtgattg caccgactgg ctccgggaaa
    10921 acgctggcag catttctcta cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc
    10981 cgcgaggcgc ataagcgtaa aacctcacgc atcctctata tttcaccgat aaaagccctg
    11041 ggcaccgacg ttcagcgcaa cttgcagatc ccgttgaagg gtattgccga tgaacggcgg
    11101 cggcgcggcg aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt
    11161 gcgcatctgg cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga
    11221 attggccttt ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcgat
    11281 cgcccggtta cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta
    11341 ccggtcgcca atatggatga tgtctcatcg gtcgccagcg gcaccggcga agacagccat
    11401 gccggccggg aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg
    11461 cgccatcgct cgaccattgt ctttactaat tcacgcgggc tggcggaaaa actgacagca
    11521 cgattaaatg agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg
    11581 gcccatttcg agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt
    11641 attgcccgct cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgaacaggcg
    11701 ctgaaatcgg gtgaattacg ctgcgtggtc gcaacctcca gtcttgaact ggggattgat
    11761 atgggcgcgg tggatctggt gattcaggtg gcaacgccgc tttctgttgc cagtgggtta
    11821 caacgcattg gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttcccc
    11881 cgtacccggc gtgatttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg
    11941 ctggaaaacc tgacaccacc gcataatcct ctcgacgtcc ttgcgcagca aaccgttgcc
    12001 gccgcggcga tggatgcatt acaggtagac gaatggtact cccgcgtacg ccgtaccgca
    12061 ccgtggaaag atctgccaag acgtgttttt gacgccacgc tggatatgct ttccgggcgc
    12121 tatccctctg gcgatttttc tgcttttcgc cccaaactgg tctggaacag ggagaccggg
    12181 atattgaccg cccgacctgg cgctcaattg ttggcggtta ccagcggcgg caccattccg
    12241 gatcgtggca tgtatagcgt gttattaccc gaaggtgaag aaaaggccgg ttcgcggcgg
    12301 gtgggtgaac tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc
    12361 ggcgctacct catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct
    12421 ccgggtcgtt ctgcccagct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa
    12481 ttaggcgaga tgatcggcga ttttcttcat ttactggcgg atggcgcgtt cttttccggg
    12541 actattcccc cgtggctggc agaagaaaat acgaacgcca atattcaggg gttgattgac
    12601 gagcaacgca acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc
    12661 cgtgatgaaa ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat
    12721 gaaccctggg cgctggcgat tgccgggcga atacatgccc tatggggcgc tgacgcgtct
    12781 gtggtcgcca gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc
    12841 gacgccgcga tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta
    12901 ggcagctcgg cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg
    12961 ccgggccgca cgccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag
    13021 ttgctggaaa tcgctcaggg atatccggat tttccggtca ttctcgaaac cctacgcgaa
    13081 tgtctgcaag atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt
    13141 ggcgaaattc aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta
    13201 ttgttcggct atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg
    13261 gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg
    13321 ggggaattac tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct
    13381 cctggtagaa gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca
    13441 atgaccgttg aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat
    13501 ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt
    13561 cttgcctgta tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg gctaccagag
    13621 tcattgccag agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc
    13681 tatctccggg ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt
    13741 attgccattg tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg
    13801 caacaaaaca tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa
    13861 gccgccagag aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa
    13921 cgtcagggcg tattacccgc caccgatggt agcccggcgc tctttgcctc aacatctcca
    13981 ggcgtttatg agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt
    14041 ttacccgcct cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgcca
    14101 gaaatgctcg atgaattact ggcaaccgga gcggttatct ggtcgggaca aaaaaaagct
    14161 gggtgaagat gacggcctgg tggcactgca tctacagaaa tatgccgcag aatcgttcac
    14221 tcccgccgaa gcggatcagg cgaatcgttc ggcgctgcag caagcgataa tcgctgttct
    14281 ggctgacgga ggagcctggt ttgcacaaca aatcagccaa cggatacgcg ataaaatcgt
    14341 cgaatcggtt gattcctcgg cactgcaaga ggcgttatgg gcgctggtct ggcaaggcgt
    14401 cattaccagc gatatttggg caccgttacg cgccctcacc cgcagcagtt ccaacgcacg
    14461 cacctcaact cgccgcagtc accgggctcg tcgtgggcgt cctgtctatg cacaacccgt
    14521 ctcgccgcgg gtatcttaca acacaccaaa tctggctgga cgctggtcgt tattgcaggt
    14581 ggagccacta aacgataccg aaaggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    14641 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    14701 gcaaacactt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    14761 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    14821 gacacaagcc acgcaaacac gccactatac accagtggcg ctctccgcca acgatccggc
    14881 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg taccaacgcg
    14941 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    15001 cggcaaaaaa atgctggtct ggcaggaaaa ggaggaatta ctcgccccag agattttcca
    15061 ggcgctgacc accgcactgc gtcgcgaacc aaggctgcgc tttacgctaa cagaagtgaa
    15121 tgatcttccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    15181 ttcgccacaa gggctggatt ggggatagtt gacatcctcc acgccctgaa agacgtggtt
    15241 ccctgctacg ttcaagctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg
    15301 cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga
    15361 gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt
    15421 gacaggcggt tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct
    15481 ggcggaacag caagcacctg accgccactc cagagctgct tgtactcaag ctggcggcgc
    15541 atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg
    15601 cgccccggct ggctgaccgt acccgctgct gactttgaca tgttgctgac cttcaaatcc
    15661 tcaatgacta tcattgcgtg gtttttgctg acggtcgttg tgactttgtg aaggtagtcc
    15721 ctgcggatat ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg cttctgccag
    15781 ttgttgctga atttgacctt ccggcttaac tgtcgctgaa gtctcgccag cgtcttctgg
    15841 tttttttgaa aactgtttac aggctcaaag actgtgccat ctgacagcgt ggcgagttta
    15901 gccacgccag catccagccc gatcattgat gctgaagggt gaaccggagt tgatacttca
    15961 ctttctgtct gaatactgat gtaccacgta ccgcaggact ggctgacagt gacatttttc
    16021 acaactcccg tgacctgacg gctattccgg tagcgcatcc agccaagttt cggcagaaaa
    16081 atacggctgt tttcctgatc gagcttaacg tcctgcgggt agcggaatgc agcattctgt
    16141 ccccgctttt tgaatcgggg aaaagcagcc cgcttctgga agaagttttt gtaagcccgc
    16201 tcaaggtctt tcagtgactg ttgcaatggc tgtgagggcg aatctttaag ccattgcgtt
    16261 tcagtggcgt ttttccactc aaccagccag gaagccattt tcccgtaagg gatgtattta
    16321 ttaccggcct catgattctc attctgaagc gccagagcac gattgaaaac gaaacgacat
    16381 gcgcctgcga agcgcctcat ttcacgctcc tgttgaccac ctggtcttaa ctggaattta
    16441 aatgcctgta atcgtttcac aggtcaaatg atactttggt ctgtgaaaaa agaaaccgat
    16501 atccggcgtg gaaggcattg tgttttcctg atgcatgttc acctggtctt tgtcaccaaa
    16561 taccgacgcc agatttttga ccatgacacg acagaaaaac tacgcactta cttttcaaat
    16621 gtatgtgctg attttgcagc tgaactggtt gaaatggatg gcgaaccaga tcacgtccat
    16681 ttgttaatta actatcctcc caaactggcg atatccagtc tggtaaacag cctcaaaggc
    16741 atatcgaata ggttactgcg acgagatcga ccagatattg cagtcaggta ttactacaaa
    16801 ggcgttttgt ggagtcctgg ctattttgcc agtagctgcg aaggtgcgcc aatatccgtc
    16861 atccgccagt acattgaaca acagcaaaca cctggtcagg tggaaaaccg cgccttatat
    16921 ccccgccctg aaggacgagg ttttacgacg caccggataa aggactgacg gatgcccgtt
    16981 cgcatccgtc agtattgcag gacggattat tccgcgtccg gctcttcaga cttgtattta
    17041 gcggcagttt ctttgatcag ctgctgcagt tcgccacgct gatacatttc gatcacgata
    17101 tcacaaccgc cgaccagctc gccgtcaacc cacagttgcg ggaaggtcgg ccagttagca
    17161 tatttcggca gttccgcacg aatgtccgga ttctgcagaa tatcaacata ggcaaaacgt
    17221 tcgccacatg cggcaagcgc ctggactgcc tgggcagaga aaccgcagct cggcagtttc
    17281 ggtgaacctt tcatgtacag caggatcggg ttttcagcaa tctggcgttg gattttttcg
    17341 atagtggtgc tcattgtctt gcttcctcaa acttctttac ggcagtaatc tgacattgta
    17401 gcgggtcagt gcggcatcgg aaaataacat tttcatcacg cttttgctat tttatccctt
    17461 tgctctattt gttgcatttc aaatattcgt ttcttctctg atgcgcggca aactggcgta
    17521 tttataacca tttttagctt tcactgctgc atttttttgc gctcgccaac gaaacgtatt
    17581 ttttaacaat aaaagctatt aactttctct tcttctatgc attagaatca tcaagttttg
    17641 taaatcagac gcaggcatga tagacctgcc ttaacagagg gacgctcagt ggcgcggata
    17701 aaccgtattt cgatcacgct ctgtgcttta ctttttacca ccctgccttt aacgcctatg
    17761 gcccatgctt caaagcaagc cagggagagt tctgctacca ctcatatcac caaaaaagca
    17821 gataaaaaga aaagcacggc aaccaccaaa aagacccaga aagccagcca gaaaacagcg
    17881 aaaaaagccg ccagtaaaag tatgaccaaa agcaaaaccg cttcttctgt taaaaaatct
    17941 tccattaccg cttctaaaaa cgccaaaact cgcagcaaac acgccgtcaa taaaacggcc
    18001 tcagccagct ttaccgaaaa gtgtaccaaa cgtaagggct ataaatcgca ttgtgtgaaa
    18061 gtcaaaaatg ccgcgtcagg aacccttgcc gacgcgcaca aagcgaaggt gcaaaaagcc
    18121 acgaaagtgg caatgaataa actgatgcag caaattggta agccatatcg ttggggcggc
    18181 agctcaccgc ataccggttt tgattgcagc ggcctggttt attacgctta taaagatttg
    18241 gtgaaaattc gtattccgcg tacggcgaat gaaatgtatc acctgcgtga tgcaggacct
    18301 atcgaacgta gtgagctgaa aaacggcgac ctggtctttt tccgtactca gggacgcggt
    18361 acagccgatc atgtcggcgt gtatgtcggc aacggcaaat ttattcagtc accgcgcaca
    18421 ggtcaggaaa ttcaaatcac ttctctcagt gaagactact ggcagcgcca ctatgttggc
    18481 gctcgtcggg taatgacccc aaaaacactt cgctaaaact ttaccctgtt gttacggcaa
    18541 cagggtaagt tcatcttttg tctcaccttt taatttgcta ccctatccat acgcacaata
    18601 aggctattgt acgtatgcaa attaataata aaggagagta gcaatgtcat tcgaattacc
    18661 tgcactacca tatgctaaag atgctctggc accgcacatt tctgcggaaa ctatcgagta
    18721 tcactacggc aagcatcatc agacttatgt cactaacctg aacaacctga ttaaaggtac
    18781 cgcgtttgaa ggtaaatcac tggaagagat tattcgcagc tctgaaggtg gcgtattcaa
    18841 caacgcagct caggtctgga accatacttt ctactggaac tgcctggcac cgaacgccgg
    18901 tggcgaaccg actggaaaag tcgctgaagc tatcgccgca tcttttggca gctttgccga
    18961 tttcaaagcg cagtttactg atgcagcgat caaaaacttt ggttctggct ggacctggct
    19021 ggtgaaaaac agcgatggca aactggctat cgtttcaacc tctaacgcgg gtactccgct
    19081 gaccaccgat gcgactccgc tgctgaccgt tgatgtctgg gaacacgctt attacatcga
    19141 ctatcgcaat gcacgtcctg gctatctgga gcacttctgg gcgctggtga actgggaatt
    19201 cgtagcgaaa aatctcgctg cataataact gatggcaaat gcagcattgc ctgatgcgct
    19261 tcgcttacca tgccaacgcg gatgaggcgt taacagcgca ttcggcaaga taaatcgcac
    19321 gttgtcagca actgtaacgc agaaggttat ccttctgcgt ttttgtttaa ttagctgtta
    19381 gcaacgcaaa ctgtttcagg ttgttttctg gctgacataa acaccagcaa taatgccagt
    19441 cccgcgacaa tcgcccccgt caccggcaca aagctgtatc ccagcccagc ggaaattacc
    19501 gcaccaccag cagctgctcc cagcgcattt ccaagattaa aggcaccaat attgactgat
    19561 gaagacagac ctggcgcttc actggcgaca tgcatcacgc gcatctgtaa cggaggtaca
    19621 accgcaaagg ttgctgcgcc ccacaccacc atgctaatag ctgcgccgaa ctcattgcgg
    19681 gccaggaacg ggattgccag cataatcacc atcagcagca acaaaaagcc tttcaacgtg
    19741 ccgttaactg aacgatctgc cagtttgccg ccgagatagt taccgataga gaatccgaca
    19801 ccaatcagca ccagcattgc cgtgacgaac accggtgttg cgtgggtaat actttgcagt
    19861 accggagaga tataggtgta gagggtaaac attgcaccag ctcccagtac cgtcgtcagc
    19921 aatgcagaca gcacctgcgg acgcattaat accgccagct cttttttcac ttcaggtcgt
    19981 gcccctgtac cacctttagg taatgagaag aacagactta ccattgaaat cactcccagc
    20041 cccgccgttg ccagaaatga catccgccag ccgatggttt cacccaacca ggtcgccgcc
    20101 ggcacgccac cgatatttgc cagggttaac cccataaaca tagtggcaac tgcgctggcc
    20161 tgtttatgtt ttggcaccac gcttgcggcc acgactgaac ccaaaccaaa aaatgctccg
    20221 tgattcaggc tggtcaaaat acgtgaaagc atcagggtca tataatccgg cgcgatggcg
    20281 gaaagaacgt tgcctagcgt gaaaattgcc atcaggaaaa tcaacgcact gcggcgggca
    20341 cgatgagaaa gtagaagcgt catcagcggc gcgcccacca ttacgccaac tgcataggca
    20401 ctgattaaca ttccggcagc gggaatcgag acatccacac cgcgcgcaat gacgggcaac
    20461 aagcccattg gcgagaactc cgttgtcccg ataccaaacg cgccaatcgc cagcgccagc
    20521 aacggatagt taattttcat gccttatctc cacctcttcg cgtcattacg cgatattcat
    20581 taaagtggcg aaagcatgac agcaatcaca aaaaaatgaa aataacaaaa agagaaaaca
    20641 cttttgccat tttgctaaca aacaggaagg agatgcgagg gagaacgcgc tccctcgaga
    20701 ggaaatcagt gcagcgcggc agtcaaaccc acggctacga tcaaaccgag gacgataatc
    20761 gttgttacca gtgaaaattt aaggtcggtg ctcatcaagt tttctccttt tttattacca
    20821 cacaaaaagt gatattacgc atttttacac actgtgatga aaaaatctcc cgtcatttat
    20881 aatgataagt gtttttacca cttccccttt tcgtcaagat cggccaaaat tccacgctta
    20941 cactatttgc gtactggcca ttgacccctt cctgacgctc cgtgtcgttt ttccggcgta
    21001 ccgcaacact tttgttgtgc gtaaggtgtg taaaggcaaa cgtttacctt gcgattttgc
    21061 aggagctgaa gttagggtct ggagtgaaat ggaatggcaa caataaaaga tgtagcgaaa
    21121 cgagcaaacg tttccactac aactgtgtca cacgtgatca acaaaacacg tttcgtcgct
    21181 gaagaaacgc gcaacgccgt gtgggcagcg attaaagaat tacactactc ccctagcgcg
    21241 gtggcgcgta gcctgaaggt taaccacacc aagtctatcg gtttgctggc gaccagcagc
    21301 gaagcggcct attttgccga gatcattgaa gcagttgaaa aaaattgctt ccagaaaggt
    21361 tacaccctga ttctgggtaa tgcgtggaac aatcttgaga aacagcgggc ttatctgtcg
    21421 atgatggcgc aaaaacgcgt cgatggtctg ctggtgatgt gttctgagta cccagagccg
    21481 ttgctggcga tgctggaaga gtatcgccat atcccaatgg tggtgatgga ctggggtgaa
    21541 gcaaaagctg acttcaccga tgcggtcatt gataacgcgt tcgaaggcgg ctacatggcc
    21601 gggcgttatc tgattgaacg cggtcaccgc gaaatcggcg tcatccccgg cccgctggaa
    21661 cgtaacaccg gcgcaggccg ccttgccggt tttatgaagg cgatggaaga agcgatgatc
    21721 aaggtgccgg aaagctggat tgtgcagggt gactttgaac ctgaatccgg ttatcgcgcc
    21781 atgcagcaaa tcctgtcgca gccgcatcgc cctactgccg tcttctgtgg tggcgatatc
    21841 atggcaatgg gcgcactttg tgctgctgat gaaatgggcc tgcgcgtccc gcaggatgtt
    21901 tcgctgatcg gttatgataa cgtgcgcaac gcgcgctatt ttacgccggc gctgaccacg
    21961 atccatcagc caaaagattc gctgggtgaa acagcgttca acatgctgtt ggatcgtatc
    22021 gtcaacaaac gtgaagaacc gcagtccatt gaagtgcatc cgcgtttgat tgaacgccgc
    22081 tccgtggctg acggcccgtt ccgcgactat cgtcgttaat cacccgttgc gggagtctct
    22141 tccggctccc gtagccactc cttattcagc gtctcactat cgccgagata ctcaagcagc
    22201 caggttaacg caggcgacat atcattttgc tgccatgtca gacaacatgc cgaatccggg
    22261 aaggggtttt ccagttcaag tgctacccac ttcccctcat taagccacgg tttggcgaaa
    22321 tgtgttggca ccatccctat gcataatcct gccgagatac aggttgccga tgattcccag
    22381 tcaggtacga caagtctttt ttggttatcc agcaaccagg taatacgttt aggtagcgtt
    22441 cgcgaggtgt cttcgcgcac caacgacggc cagttgcgca acgtatcatc gctgaacggg
    22501 ccatccatca acgccagcgg gtggtgactg gcaacaacgc aactccagct tagcatcccc
    22561 atatcccgga aggcataacg accgcctacc ggaatcgcgc gtgttgcgcc aattgccagt
    22621 tccacgcgcc cgtcggaaag cgcatcccag acaccgttga acacttcctg aaagacaaga
    22681 agttcgacat catcaaaatg gcgataaaaa tcaacgatca tctgccgtgt acgttctggc
    22741 ctgacaatat tatccactgc gatagctaac tgaccgcgcc agccgttcgc tatctgctga
    22801 cattgctggc gggtgatctg catttttttg acaacagagc gcccttcttt gagaaaccac
    22861 gctccagcag cggtcagctc cacatcacgg tgccgtcgtt caaagagcgg taccgccagc
    22921 cactcttcca gctgacgcac ggtatagctg accgcagaag gaacacgatg cagctcctgt
    22981 gccgcagcgc taaaactacc attacgcgct accgcatcaa caacttcgag tgaatattct
    23041 gaccacatag tctgcctgca aaatttttga aaccagtcat caaatattac cgtttcacaa
    23101 cactaatttc actccctaca ctttgcggcg gtgtttaatt gagagattta gagaatatac
    23161 atgcaacctg ggaaaagatt tttagtctgg ctggcgggtt tgagcgtact cggttttctg
    23221 gcaaccgata tgtatctgcc tgctttcgcc gccatacagg ccgacctgca aacgcctgcg
    23281 tctgctgtca gtgccagcct tagtctgttc cttgccggtt ttgccgcagc ccagcttctg
    23341 tgggggccac tctccgaccg ttatggtcgt aaaccggtgt tattaatcgg cctgacaatt
    23401 tttgcgttag gtagtctggg gatgctgtgg gtagaaaacg ccgccacgct gctggtattg
    23461 cgttttgtac aggctgtggg ggtctgcgcc gcggcggtta tctggcaagc attagtgaca
    23521 gattattatc cttcacagaa agtcaaccgt atttttgcga ccatcatgcc gctggtgggt
    23581 ctgtctccgg cactggctcc cctgttagga agctggctgc tggtccattt ttcctggcag
    23641 gcgattttcg ccaccctgtt tgccattacc gtggtgctga ttctgcctat tttctggctc
    23701 aaacccacga cgaaggcccg taacaatagt caggatggtc tgacctttac cgacctgcta
    23761 cgttctaaaa cctatcgcgg caacgtgctg atatacgcag cctgttcagc cagttttttt
    23821 gcatggctga ccggttcacc gttcatcctt agtgaaatgg gctacagccc ggcagttatt
    23881 ggtttaagtt atgtcccgca aactatcgcg tttctgattg gtggttatgg ctgtcgcgcc
    23941 gcactgcaga aatggcaagg caagcagtta ttaccgtggt tgctggtgct gtttgctgtc
    24001 agcgtcattg cgacctgggc tgcgggcttc attagccatg tgtcgctggt cgaaatcctg
    24061 atcccattct gtgtgatggc gattgccaat ggcgcgatct accctattgt tgtcgcccag
    24121 gcgctgcgtc ccttcccaca tgcaaccggt cgcgccgcag cgttgcagaa cactctgcaa
    24181 ctgggtctgt gcttcctcgc aagtctggta gtttcctggc ttattagtat cagcacgcca
    24241 ttgctcacca ccaccagcgt gatgttatca acagtagtgc tggtcgcgct gggttacatg
    24301 atgcaacgtt gtgaagaagt tgactgcccg aatcatggca atgccgaagt cgctcatagc
    24361 gaatcacact gatctatatc gatatactta tacttaggct gctaacaaaa ttttgttgta
    24421 tgattgaaat tagcggccta tactaatttc gagttgttaa agctacgata aatattatgt
    24481 ttttacgggg acaggatcgt tcccgactca ctatggatgg tcatttcggc aagggttcct
    24541 cctttccctc tgttctacgt cggattatag actcgcggtt ttttctgcga gatttctcac
    24601 aaagcccaaa aagcgtctac gctgttttaa ggttctgatc accgaccagt gatggagaaa
    24661 ctatgagttc atcgtgtata gaagaagtca gtgtaccgga tgacaactgg taccgtatcg
    24721 ccaacgaatt acttagccgt gccggtatag ccattaacgg ttctgccccg gcggatattc
    24781 gtgtgaaaaa ccccgatttt tttaaacgcg ttctgcagga aggctctttg gggttaggcg
    24841 aaagttatat ggatggctgg tgggaatgtg accgactgga tatgtttttt agcaaagtct
    24901 tacgcgcagg tctcgagaac caactccccc atcatttcaa agacacgctg cgtattgccg
    24961 gcgctcgtct cttcaatctg cagagtaaaa aacgtgcctg gatagtcggc aaggagcatt
    25021 acgatttggg taatgacttg ttcagccgca tgcttgatcc cttcatgcaa tattcctgcg
    25081 cttactggaa agatgccgat aatctggaat ctgcccagca ggcgaagctc aaaatgattt
    25141 gtgaaaaatt gcagttaaaa ccagggatgc gcgtactgga tattggctgc ggctggggcg
    25201 gactagcgca ctacatggca tctaactatg acgtaagcgt ggtcggcgtc accatttctg
    25261 ccgaacagca aaaaatggct caggaacgct gtaaaggcct ggatgtcacc attttgctgc
    25321 aagattatcg tgacctgaac gaccagtttg atcgtattgt ttctgtgggg atgttcgagc
    25381 acgtcggacc gaaaaattac gatacctatt ttgcggtggt ggatcgtaat ttgaaaccgg
    25441 aaggtatatt cctgctccat actatcggtt cgaaaaaaac cgatctgaat gttgatccct
    25501 ggattaataa atatattttt ccgaacggtt gcctgccctc tgtacgccag attgctcaat
    25561 ccagcgaacc ccactttgtg atggaagact ggcataactt cggtgctgat tacgatacta
    25621 cgttgatggc gtggtatgaa cgattcctcg ccgcatggcc agaaattgcg gataactaca
    25681 gtgaacgctt taaacggatg tttacctatt atctgaatgc ctgcgcaggt gctttccgcg
    25741 cccgtgatat tcagctctgg caggtggtgt tctcacgcgg tgttgaaaac ggccttcgag
    25801 tcgctcgcta aaggctattc tatcgccccc tcttcggggg cgagttcaga tcaggcttct
    25861 gtacctggtt gattcatggc attttctcgt gccgccagca cacgttctac cgtatctacc
    25921 actgcctgag tttgtggatc gatttcaatg ttgacgcgtg cgccaagttt tttcttccca
    25981 agagtcgtgc gttccagtgt ttccggaatt aaatggacgc aaaaacgcgt tggcgtgact
    26041 tcgccgacgg tcaggctaat accgtcgatg ccaataaatc ctttgtacag gatatatttc
    26101 atcaactgac tatcctggac tttaaaccag atctggcgat tattttccga ggttaatatt
    26161 ttcgccactt cagcggtggt cataatatga cctgacatta agtgtccgcc aatttcatca
    26221 ctgaatttcg ccgcacgctc aacgtttacc caatccccca cttttaaatc gccaagattg
    26281 gtaatgcgta acgtttcttt catcaggtca aaactgacat ggttgccgtt aatttccgtc
    26341 acggtcaggc agcaaccgtt atgcgccacg gaagcaccgg tttccaggcc gtccagcatg
    26401 tggtcgggta actccaccac atgcgtacga aaatttggtt tctcgtcaat cgacaccagt
    26461 tttgcggtgc cctgtacaat ccccgtaaac atacttacaa ctcctgaaat cagttaagac
    26521 attctgttca gcacaatagc aggtggaaaa cgcccttacc agtgaagggg taagaatggc
    26581 tattttttca ctggagaatt aataaatcct cgctacaata gactgaattt cccctgcttc
    26641 ttctttttgc tgcccattca ggcggctttt tagtctctca tataactaca aataaaaggt
    26701 gttcacgtgc agaagtatat cagtgaagcg cgtctgttat tagcattagc aatcccggtg
    26761 attctcgcgc aaatcgccca aactgcgatg ggttttgtcg ataccgtgat ggcgggcggc
    26821 tatagtgcca ccgacatggc ggcggtcgct atcggtactt ctatctggct tccggcgatc
    26881 ctctttggtc acggactgct gctggcatta acgccggtta tcgcgcaatt aaatggttcc
    26941 ggtcgacgtg agcgcattgc gcatcaggtg cgacaaggtt tctggctggc aggttttgtt
    27001 tccgttctca ttatgctggt gctgtggaat gcaggttaca ttatccgcta catggaaaac
    27061 atcgatccgg ctctggcgga caaagccgtg ggttatctgc gtgcgttgtt gtggggcgcg
    27121 ccgggatatc tgttcttcca ggttgcccgt aaccaatgtg aaggtctggc aaaaaccaag
    27181 ccgggtatgg taatgggctt tatcggcctg ctggtgaaca tcccggtgaa ctatatcttt
    27241 atttatggtc atttcggtat gcctgagctc ggtggcgttg gttgtggcgt ggctactgcg
    27301 gcggtgtatt gggtcatgtt ccttgccatg gtttcttaca ttaaacgcgc ccgctccatg
    27361 cgcgatattc gtaacgaaaa aggcaccgct aagcccgagc ctgcggttat gaaacgactg
    27421 attcaactcg gtttgccgat tgcgctggca ctgttctttg aagtgacact gtttgccgtc
    27481 gtggctctgt tagtgtctcc gctcggtatt gttgatgtcg caggacacca gattgccctg
    27541 aactttagtt cactgatgtt tgtgcttcca atgtcgctgg cggcagcggt aactatccgc
    27601 gtaggttatc gtctgggtca gggctcaacg ctggatgcgc aaaccgctgc gcggaccggg
    27661 cttatggtgg gtgtctgtat ggcaaccctg acggccattt tcacggtttc actgcgggag
    27721 caaatcgccc tgttgtacaa cgacaatccc gaggttgtaa cgctggctgc gcatttgatg
    27781 ttgctggcgg cggtatatca gatttctgac tcaatccagg tgattggcag tgggattttg
    27841 cgtggttata aagatacgcg ttccattttc tatatcacct ttacagctta ctgggtgctg
    27901 ggcttgccaa gcggctatat tctggcactg accgatctgg tcgttgaacc tatggggcca
    27961 gcaggcttct ggataggctt tattatcggc ctgacatcgg cagccattat gatgatgctg
    28021 cgtatgcggt tcctgcaacg tatgccgtca gccatcattc tgcaacgagc gtcccgctaa
    28081 taaagacaag gcgcaacctt cacgggttgc gcctgtgttt ttacgcaggc tggagcgttg
    28141 cgccaatccc gtcttcgtct ggctgtaatt tcagagcgtt acacagagtt tgccgactgg
    28201 catctgttat ctcaacgcgt atatccccgt taacttcccc tttcacattg atgaagtcag
    28261 gtgaaatttc tttcgcactg gtttgcaggc aaatcgctcc ctctccgcgc aggcgtttca
    28321 ctttcagttg cacatgcgtt gccgcaccag tgatcagcca cagttttccg ccattcagat
    28381 tgatgttacc aacaatgaaa gtgacgctgt caccttttac cgtactgccg tcgagaatca
    28441 acacaccgcc ctgctgtaca gtaatcgttg attgttgcag ctctgcgcca taaccaataa
    28501 tcattgaagc gccgtccagt aatgcaacac tcccggtcag ttcaaccggt gcgcgatctt
    28561 cctctacgac aaaattttcg tccgcgctgg tgggtgacaa caggatcact gttccacctt
    28621 gtattaccgc attttccagc gtaccggcat gcacttcagc ccgccccccc acattcaccg
    28681 acaggttttg cgtcttaccg gaactgtaga gctgaaggcc atccgtcccc agacgataaa
    28741 tggatccatt ctcaacaata gtgtcagagt cttcaccacc gagattattc atggtgccac
    28801 cagcaagcaa cgtatcactg gctttcccgg ccagcataaa aacgccgccc tgattaatct
    28861 cacaattgct ggcttctcca cttggataca ccaacaattc accaccttca tctaccacga
    28921 ccgctgaggt tgtcccatta accaacaggc cgccctcttg atcgagaatg attttttccg
    28981 cgcgatgtcc ttccagtaca cgcaggttac cgccattttc cagcaacaaa ccgcaggcat
    29041 aaccctgatc aacgctgaat tcaccttcgg gatggcggcc attaaccgta gcgagcgtag
    29101 agagactaat tgcgcctccc tcggactgaa caatatctgt ggcaatggca ccgtcgctga
    29161 ccgatagcgt gccattgtta ccgtactgtg tgcatttggc gatcccaccg gttgccactt
    29221 tcaaccagcc agcccccgtg accacggtat tttcggctct tccgccagct tcaacaatca
    29281 actcgccttc agtaagttgc gtcgcgccaa ggacctcgcc ggtcttaaca atttgcacat
    29341 tcccgtcgct catcaagttt ttcgcatcag atcccatgat ttattccttt gctgcatctg
    29401 tgtgccttta ttgctaccta agtgtaaagg ctacggagga tttatccacg acagatttga
    29461 gatggtggca aacaactctg tttaaactct gatacacgaa ttattgggtt gtatcagatg
    29521 taaatgcgat cctgaataaa aatcgccctt gcaaatcaac aaaatatagc caatttgctt
    29581 aaaagctctg caaacggtcg aaatagcgca gaaaattacg ttttgcctct tgccaccttt
    29641 ccactctgcc gctaatattc gtccccgttg tcacctacaa cgttgcgttc atagctcagt
    29701 tggttagagc accaccttga catggtgggg gtcgttggtt cgagtccaat tgaacgcacc
    29761 atcctgcgtc cgtagctcag ttggttagag caccaccttg acatggtggg ggtcggtggt
    29821 tcgagtccac tcggacgcac cagattttct taatctggtc ttttcctttt tccctctgtt
    29881 tcttctctgt atccaatacg ttaaaagatt tacactgtct tcgtatgcgt tatcagaagg
    29941 agaatcgcta tggcaacttt gttacaactt cattttgctt ttaatggccc atttggcgac
    30001 gcaatggctg agcagcttaa accacttgct gagtcgatta atcaggaacc tggttttctg
    30061 tggaaggtat ggacagaaag tgaaaagaac cacgaagccg gtgggatcta ccttttcact
    30121 gatgaaaaaa gcgcccttgc ctatctggaa aaacatactg cccgactgaa aaatctcggc
    30181 gttgaggaag ttgtcgccaa agttttcgat gtcaatgagc cacttagtca aatcaatcag
    30241 gcaaaactcg cctgacagaa tttaatcaag ggcggttagc gcccttttca tccctgtctg
    30301 aaatttctca aattctaaaa atctcaacca aacttatctg ataacactaa attcgaaaga
    30361 atgcgtacag gtaagtaaca atgaaaaaaa ttgctattgt gggtgccggg cctacgggga
    30421 tctacacctt attctcgctt ctacagcaac aaactccact ttctatttct atcttcgagc
    30481 aggctgacga ggccggtgtc gggatgccat acagtgatga ggaaaactca aaaatgatgc
    30541 tggcaaatat tgccagtatt gaaataccgc cgattaattg tacgtatctc gaatggctac
    30601 aaaagcaaga agccagccat ctccagcgtt atggcgttaa aaaagaaacc ttgcacgatc
    30661 gtcagttttt accgcgaatt ctgctgggcg aatatttccg cgatcaattt ttacgattag
    30721 tagaccaggc acgacagcaa aaatttgcag tggctgttta tgaatcatgc caggttaccg
    30781 atctgcaaat tacaaatgct ggcgtcatgc tcgctacaaa tcaggattta cccagcgaga
    30841 cgtttgattt agcggtgatt gccacgggtc acgtctggcc tgatgaagaa gaagcaacca
    30901 gaacgtattt tcccagcccg tggtcaggcc tgatggaagc aaaggtcgat gcgtgtaacg
    30961 tgggtattat gggaacatcc ttgagcggac tggatgcggc aatggcagtg gctattcagc
    31021 atggttcgtt cattgaagat gataaacaac acgtcgtttt tcaccgcgat aacgcaagtg
    31081 aaaagctaaa tattacgttg atgtcgcgca cgggtatttt acccgaagcc gatttctatt
    31141 gccctattcc ctacgagccc ttacacatcg tcaccgatca ggcattaaat gctgagattc
    31201 aaaaaggtga agagggcctt ttggatcggg tatttagatt gatagtagag gaaatcaagt
    31261 ttgctgatcc agactggagc caacgcatag ccttagagag cctgaatgtc gattcctttg
    31321 ctcaagcctg gtttgccgag cgcaaacaac gcgacccatt tgactgggca gaaaaaaatc
    31381 tccaggaagt cgaacgcaat aaacgagaaa aacatactgt tccctggcgt tatgtcattc
    31441 tgcgcctgca tgaagccgta caggaaattg ttcctcatct gaatgaacac gaccataaac
    31501 ggttcagtaa aggccttgcc agggttttca tcgataatta tgcggcaatc ccttcagagt
    31561 ctattcgtcg actactggcc ttacgtgaag cggggatcat tcatattctc gccctcggtg
    31621 aagactacga aatggaaatt aatgagtcgc gcaccgtcct gaaaacggaa gacaacagct
    31681 actcgtttga cgtttttatt gatgcccgcg gacagcgtcc gcttaaagtg aaagatattc
    31741 ctttccctgg gctacgcgag caattacaga aaacagggga tgaaatccct gatgttggcg
    31801 aagattatac gttacagcaa cccgaagata ttcgtggacg cgtagcgttc ggcgcgttgc
    31861 cctggttgat gcacgaccag cctttcgttc agggacttac ggcatgtgca gaaattggtg
    31921 aggcgatggc tcgggcggtc gtaaagcctg catcccgtgc tcgtcggcgt ctttcgtttg
    31981 attaaaggta aagctatcag cactcaacaa actgatgctg ttcattaaga cgatagcgga
    32041 ctccggttcg aatattgttt tcaccttcaa tggcgacggc gaagcgaact ctttcgccgt
    32101 catgatatgc cagtgcggcg cttcccccta gcccgagaat aattgagtcg accacgcccg
    32161 tgctcactac cgttgaattt tcaccgctgg caatgatgtg tacattgtcg ccgctgttgg
    32221 caattctggc gtttgcgcca aagctggcga tttgcaccag gtcaccatta ctggcgacat
    32281 gacaccgttc acccaatgta caaacgcgta ctcgcattcc ggtattcgca atccgactac
    32341 tatctccagc gctgcttatc cgcgaagaat taccggttac agcaacccgt gcgcgttcac
    32401 cggagctgcc aatgtgactg ttaaagccga cacttccaat ccgcacgcta taacccatac
    32461 tggcaatttg cgcggcatac ccagcggttg cgattcgggc gttatcctca caacagccga
    32521 tgcgagcatg atcgtcgctg cgggcaaact gactgcaaaa tggtatctct ccggggtctg
    32581 tggccagttt ttgcatagag gaaacttcct gatgcgcgaa gttttcatcc gccagccatt
    32641 ttttccacgc atactcaacc agactctccg cccagtccgt atatccttcc tcaatcagtg
    32701 catgacgaat atcggcataa ctaccgccgc gtgggaaatg gcgcaggaac cagcgataca
    32761 tcaccgctcc gatacgccat tctcgcaggt ctgctcgtgt aatgatcatt cgttagtgcc
    32821 ggtgatagcc atcgaccatg cttttaaagg tttcgtgtgg cgtacgcccc gtggtgcaaa
    32881 gataaagatg accgaagata aaaaagaggc ttataaatgc cagagcaaaa tgcgcctgca
    32941 ataaccagta tcttacgcca ggaaacacat ctcccacggc ttgcggatag agacacaaca
    33001 gccccgtcaa tagtaacaac ggcagcaatc catacatgac accaacatag gcgacctgct
    33061 gtaagggatt aaatttagac tgtgttgttg ccgggaaagg atgttcttcc ccctgcataa
    33121 tgccaaacaa ataaaatcgc gtttgttttg ccgctcgttc cagccacccc tgacgacgaa
    33181 tgcgatagtg gtgaccatta tccccaacgg cattgatcag cacaaagccg agccagcatg
    33241 ccagtaacaa aaatccgcaa acttcatgca ccgcaaccag acttttaacc gcagttgcgc
    33301 ccaccatcgc aaagtgattt atcagcccac ttgccagcaa caatacaaag agtaacgcat
    33361 tcgaccagtg ccataatctg accgctttgc tgtaaagata aaccttctca ccatgatcgg
    33421 tggctgattt cttcacgcca cgagcacgaa gcaaggcgtg cagccccagc accaaccaca
    33481 tcccaaccag caatactccg gcaatgatca accacaccgg ccaaaattcg ggagtgaact
    33541 gtggcacata gttcgctaac tggctctgaa actgttcagc atgttgcgac gggttcatac
    33601 attttccttt ttaatcaaat gttgaccgaa ccggcgatac agatgcggtt ttccggcacc
    33661 aggtagctga tattgatagt atttattatc ctgcaaccac gcctgaattt ctgggctatc
    33721 ttcgcgacca aagatcaatg cgtgttccgg acaggcgctg acgcaaatgg gcggaaagcc
    33781 ttttgccagt cgggactcag cgcagaaatc acatttatct gccactttag tgacagggtt
    33841 aaggtagcga acctggtacg gacaagcgcc aatgcaataa ctgcagccga tgcactgcga
    33901 tttttctacc cgcacgatcc cctgctcatc acgccacgat gcccccgtcg ggcatacgtc
    33961 gatgcacgga gcatcttcgc agtgctgaca tgactggcga aaaaagtgat actgcgtttc
    34021 gttgtcgtta tcggtgactg gaatgtgtgc tatcgataaa cggcttccct gagcggggac
    34081 gtggttagtt ttacggcatg cgcgggcgca aatattacag ccattgcacc gtgactcgtc
    34141 atgaatcatg gcataccgaa cttccttttc ttgcctcgtg ttcgctaaca gagaagacgc
    34201 agaaccagta aaaaagatta ctgttcccat ccccagaaca aatttgcgtc gagtaaagga
    34261 catctgatta tcccctaatg cagtggctgc aactgtccac cctatccatg acatctccct
    34321 gaaatttcag gggttctgaa actgctctac ctcgaaccat tcgcataagc cataagcctt
    34381 gccataggtc ataaatcgct cgctaaacaa gtacaacacc gccgtcgatg actccacctg
    34441 ccgtatatcg gcatattcag gtgcaacatc catcgcggcg atcgcggctt caatctgcgc
    34501 ttcctggaag taatatggcg cttgcatcag catggcgact ttgtaaggac gaggatacgt
    34561 ctggcactca aaacgaacgg cgtgtgcaat ggcgcgacaa atgtcctgct ccacaacttg
    34621 caaagacatc gccgcgtagt tttcactcat ggcctgcgtt gagtaatagt aatcatcctg
    34681 acttcccgtc atacaagcga tatccgcgta gtctccgttc tgcttaatgc tttccagcaa
    34741 aactgacagt tcctcttctg caaccgaata gggtggcgag agaaacacgg tgcgcgccac
    34801 cagctgccca gccgcagaat gctggcgaat gaactcagcc aggatttcag caggttctgg
    34861 ttcggttacc gtatcaacgg ggtgacgcat cccctgcaac cattgccgct tcgcctcgtc
    34921 aacttcgctg actttcgcta acggcaattc atcctgatgg ttcatcttac ccattcctta
    34981 cgcaggcagt agattgtgtg ctgccagatc ggcggcaata tcttccagcc ccagacgctg
    35041 caatgtttcg cgggttggac aaccaagctg tgggtcccag cccatttctt tgtagaacat
    35101 cgtgagcgaa gcatgcatat cgtcacgatc cattttgtca gtaccttcag taaagaccgg
    35161 gatctgcgga tccttgtcga aaacccagga acagataaga tcgtgttcat tacgcatatc
    35221 tttggtttgc atcggtttta ccgtgtaggc acgatgcaac gtaaagatac gctctgcagc
    35281 taaatctaat ttttcctgag tcatctcttc gccggtgatc gctttgaaga atttggctta
    35341 aagcgccaga tcgccccggt aattacggct tttcagcggc gaaacggtca ttggccagac
    35401 ccagttgcac agagtgacgg cgttatgcaa acagacccgc aacagcgacc actttgcata
    35461 tttaattttt gcgtcattga ttggcgtgta gtttttggtt tcatcgtaag catcttcaga
    35521 accaaaaagt tctttcgcca cttcacgttg cagtttcaat ggcaagccgg aaccaataaa
    35581 gttgatatgg gtatgcgtca tgcagtcacg gttgaacata cagttaacaa tggaaccgac
    35641 ctgcgctgac gcttcattgg catggtgaac cggatagcca aacggcgacc agagtttatt
    35701 tttcgcgtag ccccagtact ctttacccaa attccagcgt tctgcgatgg catatgaacc
    35761 atcagccagg tgactcagct cacccacacg atgcgccaga cggtagtaaa aatctttaat
    35821 gaagttaacg tcacccgctt ccagttgatc ccagcgaatt tctgcatact cttcagctgg
    35881 cagaacacgc ttgaacacac ctttgctgta acaataagta aagtcgcgat gcaactgccc
    35941 gtagttacac cataggccgt agtcatcgaa caaattcaga ccaaccaggt taccaatcac
    36001 acgaccatcg tctttatctt caaaatcttt cggcccgttc gggaagatgg tggtatggac
    36061 aaagtttgct acacaagtgt taccacctgt gctgggcacg ccaaactctt tcacccgagg
    36121 aatattcatt tgggtcatac aacggatcgg gcaagagtgg cagccgctca tttttactgt
    36181 gtatttctct gccgccggtc ctaagtcaaa aacggattta taggtacgaa agccgaccgt
    36241 attctgattg cctggcggaa tttcacccgt ttcaatcgga ccaccttcag ccgcgcccca
    36301 aaacagccct ttacgtgctg tccagcgtga cttgggatct gaatactctg cccacgattg
    36361 tggcgtactt ggcacgacat ggttgttatt cgcaccaata agttcagtca tcatgtaatc
    36421 attgagacgc ttcatctcct gacgatcggc aatgttgacc cctttcgtcc cttcaaccgc
    36481 aatcgctttc aggtttttcg aacccattat tgcgccagtt cccgcaccgc cgctgtggtt
    36541 acggctattc aacatgccag agagaggaat aaggttttcc ccagcctgac caatagccgc
    36601 cacacaggtt tccgggctgg tcaatcgaca aatttcttcc gtcgttgcgc gcgtcccttt
    36661 tccccataag aaatcggctt tttccaggct aactttgtca tctttaatct tcagccatac
    36721 cggtgatttc gccttccctt cgataataat gacgtcgtat ccagcgaatt tcatttgcgc
    36781 tgcaaaaaag ccacccatat gggcatcgcg actaaatttc ctttggtaaa agtagaaagt
    36841 gaggtgatat ttacgcgaga actacagggg gcaccagatc cagttaatgg gccggtagca
    36901 aagactaatt tattcgcttc atcgaaaggt ttcgtgccta gcggtacttc gtcatacata
    36961 attttgtagc cgaagcccat gccaccgaca aaacttttaa acttactgga atcttcgagg
    37021 gtaatatttc ctgtcgtgag attgactctt aatatattac ctgtccaacc gttagccatg
    37081 attttttcct ttgcaagatt cacacagtaa tatctttcca ctcgataatt tttaacgccc
    37141 ctgttgggca ggcgtttgcg cattcaccgc ataacacaca tttcgaggat tttttacttt
    37201 cggtatttac ggtggccatc atccacgggc atgccgtagt acaggcgcta cagccaatac
    37261 aacgtttatg atcgacggta atacagcctt ctttctgctg ccaggtaatc gcaccaatcg
    37321 ggcagacgtt catgcattgc ggctctttgc attgacggca ggtgtccgcg gtatagttga
    37381 gatcgccata caggccgccg ccagagccaa ccccgttgtc gccaaagaaa taattgcgat
    37441 ggattttgat acgggagaag aatgtcccta ctgagccatc gttgaagttg gtacaagaga
    37501 tttcacagcg gtggcaaccc gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg
    37561 tatttatcaa ccctactgtg ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca
    37621 gtaacgcagg ggcaattgtt agaccagcaa ggccttttcc tgatatacgc aagaattcta
    37681 atcgcgttaa gccaatatct aatagtggac gatcaaccgg gttcatttat ttcgcatcct
    37741 cttcgcaaac acagtgctga caattcagca gcctgatatt aagcattcaa ctaatacgct
    37801 gctgactctg tccccaacac cagatatttt ccctaacccg atagtgtatg agaaaagatt
    37861 taacgccgat aaactaatcc tttatggtta ggagaaaaaa taatgtgatt attctgagcc
    37921 cttaacattg atcgttatca attaaaatta caaagcaaga aattataagc gaactgatat
    37981 ttattatcat ttgaaataaa tttaacttaa gcgacaatca tttgtgacgt tcattgcaga
    38041 aaataaaaat aacattatcg ctataaatta aaatacatac cgaaattcgt ataatttcag
    38101 cagttgttat ttagaatgat taactggacc gcaactgaag caagaatatc aggatgaagc
    38161 ttaccgcctc atcctgaatg gtcattttac cagtgcgtta atgacggttt ccatcgcctg
    38221 cttttccagc tcactgcgct gaatgcgttt attggctaat gctgtacgta atacgcctgc
    38281 gataacaata tgctttggtt cctgccccag atcgcgcatt tccagcacaa ctttaccgac
    38341 aactctgcac atttctcggt acagttcatc ctcttttgtc cgatttccca ttctttatac
    38401 ctatttatca tgccaactat caggtaatgc tgccaactta ctgatttagt gtatgatggt
    38461 gattttaagg tgcttgcgtg gcttccattt ccatcagatg tccttcctgc tccgctactg
    38521 aaggcgtggt
//
LOCUS       DAECNO010000030        37629 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.030, whole genome shotgun sequence.
ACCESSION   DAECNO010000030 DAECNO010000000
VERSION     DAECNO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.030"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..176)
                     /locus_tag="KKO08_001573"
     CDS             complement(<1..176)
                     /locus_tag="KKO08_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBD6317865.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="KKO08_001574"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="KKO08_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBD6317866.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="KKO08_001575"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="KKO08_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBD6317867.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="KKO08_001576"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="KKO08_001576"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBD6317868.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="KKO08_001577"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="KKO08_001577"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBD6317869.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="KKO08_001578"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="KKO08_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBD6317870.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="KKO08_001579"
     CDS             complement(5180..5422)
                     /locus_tag="KKO08_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBD6317871.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="KKO08_001580"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="KKO08_001580"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBD6317872.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="KKO08_001581"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="KKO08_001581"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBD6317873.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="KKO08_001582"
     CDS             complement(8339..9313)
                     /locus_tag="KKO08_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD6317874.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="KKO08_001583"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="KKO08_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBD6317875.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="KKO08_001584"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="KKO08_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBD6317876.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="KKO08_001585"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="KKO08_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBD6317877.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="KKO08_001586"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="KKO08_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBD6317878.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="KKO08_001587"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="KKO08_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBD6317879.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="KKO08_001588"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="KKO08_001588"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBD6317880.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="KKO08_001589"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="KKO08_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBD6317881.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="KKO08_001590"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="KKO08_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBD6317882.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="KKO08_001591"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="KKO08_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBD6317883.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="KKO08_001592"
     CDS             23180..23383
                     /locus_tag="KKO08_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBD6317884.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="KKO08_001593"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="KKO08_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBD6317885.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="KKO08_001594"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="KKO08_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBD6317886.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="KKO08_001595"
     CDS             26388..26660
                     /locus_tag="KKO08_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBD6317887.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="KKO08_001596"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="KKO08_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBD6317888.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="KKO08_001597"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="KKO08_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBD6317889.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="KKO08_001598"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="KKO08_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD6317890.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="KKO08_001599"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="KKO08_001599"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBD6317891.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="KKO08_001600"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="KKO08_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="KKO08_001601"
     CDS             complement(31907..32305)
                     /locus_tag="KKO08_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBD6317892.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="KKO08_001602"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="KKO08_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBD6317893.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33846..34274
                     /gene="rplM"
                     /locus_tag="KKO08_001603"
     CDS             33846..34274
                     /gene="rplM"
                     /locus_tag="KKO08_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBD6317894.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34290..34682
                     /gene="rpsI"
                     /locus_tag="KKO08_001604"
     CDS             34290..34682
                     /gene="rpsI"
                     /locus_tag="KKO08_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBD6317895.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35077..35715
                     /gene="sspA"
                     /locus_tag="KKO08_001605"
     CDS             35077..35715
                     /gene="sspA"
                     /locus_tag="KKO08_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBD6317896.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35721..36218
                     /gene="sspB"
                     /locus_tag="KKO08_001606"
     CDS             35721..36218
                     /gene="sspB"
                     /locus_tag="KKO08_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBD6317897.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36261..37628)
                     /gene="dcuC"
                     /locus_tag="KKO08_001607"
     CDS             complement(36261..37628)
                     /gene="dcuC"
                     /locus_tag="KKO08_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBD6317898.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9115 a         9417 c         9897 g         9200 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatacc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgtttg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gtctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 caggcaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacttggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctaggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacagcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttccccaaa
    33721 actttttgtg tgctggcata ggctattcga aggggtaggt ttgccggact ttgtcgtgtg
    33781 aacctcaaca attgaagacg tttgggtgtt caccaacgtg taactattta ttgggtaagc
    33841 ttttaatgaa aacttttaca gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg
    33901 acgcgaccgg taaaactctg ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta
    33961 agcacaaagc ggaatatact ccgcacgtag ataccggtga ttacatcatc gttctgaacg
    34021 ctgacaaagt tgctgtaacc ggcaacaagc gtactgacaa agtgtactat caccacaccg
    34081 gccacatcgg tggtatcaaa caagcgacct ttgaagagat gattgctcgc cgtcctgagc
    34141 gtgtgattga aatcgcggtt aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt
    34201 tccgtaaact gaaagtttac gcgggtaacg agcacaacca cgcggcacag caaccgcaag
    34261 ttcttgacat ctaatcggga ttataggcaa tggctgaaaa tcaatactac ggcactggtc
    34321 gccgcaaaag ttccgcagct cgcgttttca tcaaaccggg caacggtaaa atcgtaatca
    34381 accaacgttc tctggaacag tacttcggtc gtgaaactgc ccgcatggta gttcgtcagc
    34441 cgctggaact ggtcgacatg gttgagaaac tggacctgta catcaccgtt aaaggtggtg
    34501 gtatctctgg tcaggctggt gcgatccgtc acggtatcac ccgcgctctg atggaatacg
    34561 acgagtccct gcgttctgaa ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg
    34621 ttgaacgtaa gaaagtcggt ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt
    34681 aattggcttc tgctacggca gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt
    34741 tatggctaaa ttctgaatca gcgtaaaaaa tgggaagttg ttttttgctg ccgtctgaaa
    34801 gacaggtaaa acaaaaacca catcgccaat aagggtctaa gtcaactatt tcagactaaa
    34861 gcgcatctct ttttccccat ttccggcatc cactcaccac aaaggtcgca aaatctggta
    34921 aactatcatc caattttctg cccaaatgtc gggtattgct cattttttgt ttgattttcg
    34981 aacaaagaga gtcgttcctt attgggtaac acaacttctg actggccacc tggtggctgg
    35041 tagcagtaaa aattctgact atacctggag gttttcatgg ctgtcgctgc caacaaacgt
    35101 tcggtaatga cgctgttttc cggtcctact gacatctata gccatcaggt ccgcattgtg
    35161 ctggctgaga aaggtgtaag tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag
    35221 gatctgattg acctcaaccc gaatcagagc gttccgaccc tggtggatcg tgagctgacc
    35281 ctgtgggaat ctcgcatcat tatggaatat ctggatgagc gtttcccgca tccgccactg
    35341 atgcctgttt acccggtagc tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa
    35401 gactggtaca cgctgatgaa cactatcatc aacggttcag cttctgaagc agatgccgca
    35461 cgtaagcaac tgcgcgaaga actgctggcg attgcgccgg tcttcggtca gaagccgtac
    35521 ttcctgagcg atgagttcag cctggtcgat tgctaccttg ctccgctgct gtggcgtctg
    35581 ccgcaactgg gcatcgagtt cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc
    35641 cgcgtctttg agcgtgactc tttccttgct tctttaactg aagcagaacg tgaaatgcgt
    35701 ctgggccgga gttaatctgt atggatttgt cacagctaac accacgtcgt ccctatctgc
    35761 tgcgtgcatt ctatgagtgg ttgctggata accagctcac gccgcacctg gtggtggatg
    35821 tgacgctccc tggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca
    35881 acattgcgcc gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggtg cgctttaacg
    35941 cgcgctttgg tggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct
    36001 acgcccgtga aaatggcgca ggcacgatgt ttgagcctga agctgcctac gatgaagata
    36061 ccagcatcat gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttattg
    36121 atggcgacaa gccagatcac gatgatgaca ctcatcctga cgatgaacct ccgcagccac
    36181 cacgcggtgg tcgaccggca ttacgcgttg tgaagtaata caaaacaggc ccaggcggcc
    36241 tgttttgtct ttttaatgac ttaataaaat agcgtgattg tggcaatcat attcaccacg
    36301 aaaccgactg ccatggggat cgctgtccgc ttcaccaccg caaaaggcga aacgcctgca
    36361 attcctgacg taacgaccac caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg
    36421 gcaaaatgca tcggcataat cattacaacc gctggtacat gtagtccggc tgcgatattc
    36481 ggaataagac tggcaaatga cataaacggc gcattgccag agcccatcac aatggcacaa
    36541 atggcaatga ctagcgccat aataatcatc acgccaatac cgcccagacc agaatgctcc
    36601 gcacccctga taaccgcatc aacagtgcca atcgtcgtta agcctttcgc aaatatttca
    36661 cccgcgacca ccagcgttac cacgttgaca aactgcgtac ccatgccgtc aaaaaacgcc
    36721 tgcacatcgt ccattgtttc gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc
    36781 accgtcaaac ttagtaacat caccaccacc agatgcagtt ctgacttcat cagcccgacg
    36841 tgggcgagga acagcgagcc gagcatcagg attaacggca tcacaggtaa aatggcgtaa
    36901 tagagcggcg ggacattatc gagagttttt tgctctgcct gttcgtgatt gatattttta
    36961 tcttttttgt caaaagcgcg ttgcacgaaa aagtgggaga tcgccaccga gataatgacg
    37021 caagaggcga ccggaagctg gtagtggaag aagtatgtgg caattttcat tcccgcgacc
    37081 tgggctgcaa aaatggagtt cgtttccaga atcccccact caatggacat cgtggttgcg
    37141 ataaccgcca ccgcagagag gcgacttact cccagactca ccagcgtcgg aaataaggtg
    37201 accatcagca acatacccag accggaggcg ctggtaataa actgcgccat gatttggccg
    37261 atgacgtaag ttgccgacag aataatatac ggcgagcgaa tgagttttaa cgggcggctt
    37321 agcaagctca ccatcgcgca actggctcct atgcgctcca tgtagcgggc ataaccgccc
    37381 accgccataa tcgacagccc caatccggca atgcggttgg acagcatgcg caggatttca
    37441 ttataaatgt cgacgatcaa gtagccgctg cttttggcgg gatcgagtac accaccgaac
    37501 cctaaccaga ctccgcacat catcaggaag acacctgcgg cagccagcac cacctgaggt
    37561 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg
    37621 ccgaacatt
//
LOCUS       DAECNO010000031        36581 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.031, whole genome shotgun sequence.
ACCESSION   DAECNO010000031 DAECNO010000000
VERSION     DAECNO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.031"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KKO08_001608"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="KKO08_001608"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBD6317899.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KKO08_001609"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="KKO08_001609"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBD6317900.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="KKO08_001610"
     CDS             complement(3122..3421)
                     /locus_tag="KKO08_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBD6317901.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="KKO08_001611"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="KKO08_001611"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBD6317902.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="KKO08_001612"
     CDS             complement(4633..5070)
                     /locus_tag="KKO08_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBD6317903.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="KKO08_001613"
     CDS             complement(5116..5457)
                     /locus_tag="KKO08_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317904.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KKO08_001614"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="KKO08_001614"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBD6317905.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KKO08_001615"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="KKO08_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="KKO08_001616"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="KKO08_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6317906.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="KKO08_001617"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="KKO08_001617"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBD6317907.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="KKO08_001618"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="KKO08_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBD6317908.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13865
                     /gene="xylR"
                     /locus_tag="KKO08_001619"
                     /pseudo
     CDS             12688..13865
                     /gene="xylR"
                     /locus_tag="KKO08_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
     gene            complement(13973..14797)
                     /locus_tag="KKO08_001620"
     CDS             complement(13973..14797)
                     /locus_tag="KKO08_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBD6317909.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="KKO08_001621"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="KKO08_001621"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBD6317910.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="KKO08_001622"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="KKO08_001622"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBD6317911.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="KKO08_001623"
     CDS             complement(18729..19202)
                     /locus_tag="KKO08_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBD6317912.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="KKO08_001624"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="KKO08_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBD6317913.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            20353..20712
                     /locus_tag="KKO08_001625"
                     /pseudo
     CDS             20353..20712
                     /locus_tag="KKO08_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
     gene            complement(20750..21886)
                     /locus_tag="KKO08_001626"
     CDS             complement(20750..21886)
                     /locus_tag="KKO08_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6317914.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(21892..22215)
                     /locus_tag="KKO08_001627"
     CDS             complement(21892..22215)
                     /locus_tag="KKO08_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD6317915.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            22398..22541
                     /locus_tag="KKO08_001628"
     CDS             22398..22541
                     /locus_tag="KKO08_001628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317916.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(22760..24298)
                     /gene="aldB"
                     /locus_tag="KKO08_001629"
     CDS             complement(22760..24298)
                     /gene="aldB"
                     /locus_tag="KKO08_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBD6317917.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(24463..25614)
                     /gene="yiaY"
                     /locus_tag="KKO08_001630"
     CDS             complement(24463..25614)
                     /gene="yiaY"
                     /locus_tag="KKO08_001630"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBD6317918.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(25804..27648)
                     /gene="selB"
                     /locus_tag="KKO08_001631"
     CDS             complement(25804..27648)
                     /gene="selB"
                     /locus_tag="KKO08_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBD6317919.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(27645..29036)
                     /gene="selA"
                     /locus_tag="KKO08_001632"
     CDS             complement(27645..29036)
                     /gene="selA"
                     /locus_tag="KKO08_001632"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBD6317920.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(29134..29742)
                     /locus_tag="KKO08_001633"
     CDS             complement(29134..29742)
                     /locus_tag="KKO08_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBD6317921.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(29813..30898)
                     /locus_tag="KKO08_001634"
     CDS             complement(29813..30898)
                     /locus_tag="KKO08_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6317922.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(30901..31263)
                     /locus_tag="KKO08_001635"
     CDS             complement(30901..31263)
                     /locus_tag="KKO08_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBD6317923.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            31800..33713
                     /gene="mtlA"
                     /locus_tag="KKO08_001636"
     CDS             31800..33713
                     /gene="mtlA"
                     /locus_tag="KKO08_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBD6317924.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            33808..34956
                     /gene="mtlD"
                     /locus_tag="KKO08_001637"
     CDS             33808..34956
                     /gene="mtlD"
                     /locus_tag="KKO08_001637"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD6317925.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            34956..35543
                     /gene="mtlR"
                     /locus_tag="KKO08_001638"
     CDS             34956..35543
                     /gene="mtlR"
                     /locus_tag="KKO08_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBD6317926.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(35555..35764)
                     /locus_tag="KKO08_001639"
     CDS             complement(35555..35764)
                     /locus_tag="KKO08_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317927.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            36048..36410
                     /locus_tag="KKO08_001640"
     CDS             36048..36410
                     /locus_tag="KKO08_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBD6317928.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         9105 a         9507 c         9030 g         8939 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgtcttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgggc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcgcccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact gtttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatat acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacgcctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgttgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgctca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaaaca
    20341 aggaagccta ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcagcaactg cggcctgaac aagcggcgga aagcgaagat taatgatcga
    20761 gataaaggta atgcacccag ctggtcatgc gtaatagcac tttgcgcatc accgagacat
    20821 ggctgaaatg atcagagtag accgccacct gggcgtaaat accgtctggt aaagcgctca
    20881 aatcagtgtg ctcatccagt tcaatggtcg cgataacgcc atctgaaccc ggcgctgtgt
    20941 ttaacgtctg taaggtgccg gtcgactgat aagctccgcc gggaacagca ggactaatgg
    21001 ctgccagctt gccgctgaat accttacctg gcagagcatt aaacaccact tccgcatcgt
    21061 cgccaggggc cagtcgcagc aaggagttct gacggaactg cgccacaatt tgtcgtttct
    21121 gatcgggtat aaacaccatc accggacgta gcggcagcga cgcggcatag gtacccggac
    21181 gaatcagcac ctgggtcaca taaccatcgc tcggcgcacg cactatcgtc tgctcaaggt
    21241 tatattttgc ttccgcgagc tgcgctttca ggctggcgat ttgagaatgt tcacccaaca
    21301 ccaggctatc cagctggctc tggatctgtt tttgttccgc cgccgatgac tttacggagg
    21361 cttcctgcgc cagataattt tgccgcgcca catcaatatc gcgttctgaa aacgggttta
    21421 ctttcgcctg gctgccgcgt gcgtaacgct gatactcttt agcgaattta tcccgcgtgg
    21481 cctttgcctg ctgagtattc gccgccatct catctaactc tgcgcccaac gcccgctgtt
    21541 tatgttctgc ggtaacgata tccgccatca gccgatccac ccgcgcctga taacgcgtcg
    21601 ggtccagtcg aaatagcacc tcaccttttt taatcagcgt atttttctta tccgtcactt
    21661 cgatcaccac gcctgtcacc tgtgggacaa caggaataga aataaccgct ttttgcgcct
    21721 tgaaggtata cggatggtta tagttcatta acagaattaa accactgaca ataaatatgc
    21781 cacccagggc cgctgtggaa atggtccatt tatttacagg aattttgaag atcttaaaca
    21841 ttgcccatgc aaaagccaca taggtcaaaa taatcagtaa atccataagt attactcggc
    21901 agaagaggat ttgatgtcgg cgagctgttt ctccagcccg gcaatacgct gttgtagttg
    21961 cacaacggag gagtcatgga tttgcattcc ccatccccgc tccgggcggt aaagcgtggc
    22021 ccaaatccac aaaaatggcc agataacatg cagcgtaaaa agactcaccc aaccagcaac
    22081 atgaatggcg tcggcatgag gatgattacg ttttttagca atcaggtagg gaatatcatg
    22141 taaaataata atcccataga atatcaccag aaatacaaaa ataagcactc ccagtgcaaa
    22201 atagtccagg aacatatttc cctcaaagaa tataaaaaag aacaattaac gcatattatg
    22261 aaatgccatg atgcagtgca atttccttat ttatataacg acatattgtt ttttatatac
    22321 tttctgaaat gtgctgtctg gtttttcaac aggacgaggt ttatcatatt gaaatagtgg
    22381 aactattggc caaactaatg aataactcca gtaaaacatc cacagtacag attaagcgta
    22441 ttaaaccttc aattatctac cgtttattgc tgattggcct cggatcacca atggtgattt
    22501 acggcctggt tcgcccgctc accatcgaaa cgcgagatta aactcctgac aaaacacgcc
    22561 ccagaaacgc acttctgttg gcgtagtctg ggttattgcg cacgtaggtt taaaacgtaa
    22621 attgtaacaa cgtgcgcttt gtttatgccg gatgcggcgt gtacgcctta tccggcctac
    22681 ggtcaggttc ccgtaggcat gataagacgc gtaagcgtcg catcaggcaa tgaataccca
    22741 atgcgaccag cttcttatat cagaacagcc ccaacggttt atccgagtag ctcaccagca
    22801 ggcacttggt ttgctggtaa tgctccagca tcatcttgtg ggtttcgcga ccgatacctg
    22861 attgtttgta gccaccaaac gcagcatgtg ccgggtaagc gtgataacag ttggtccaca
    22921 cgcgcccagc ctgtatgccg cgccccatct tataggccag attaccgttg cggctccaga
    22981 cgcccgcgcc caggccatat tgcgtatcgt tcgccagctc cagcgcttct tccatcgttt
    23041 tgaaggtggt caccgccagc accgggccaa aaatctcctc ctggaacacc cgcatattgt
    23101 tctgaccaaa cagaatcgtc ggttcgaggt agtagccgtc tttcagttca ccttccagca
    23161 gcttgcgccg cccgcctgtg agcacgtcag cgccctcttt tttaccgata tcaatgtagt
    23221 tgaggatggt ttccagttgc ccgtgagaaa cctgcgcgcc catttgcgtc acgctgtcga
    23281 gcgggttacc gctgcgaatg ctttcgacac ggcggatggc gcgttccata aagcgttcgt
    23341 agattgactc ctgcactaag gcacgactcg gacaggtgca aacttcgccc tggttaaagg
    23401 caaacagtgc aaagccttcc agcgctttat cgaaaaaggc atcttcttca tccatcacat
    23461 cagcaaagaa gatatttggc gacttaccgc ccaactccag cgtcaccgga ataatgtttt
    23521 gcgttgcgta ttgcataatt tgttggccca cttccgttga gccggtaaac gccactttgg
    23581 cgatgcgttt cgaggtcgcc agatattcgc caatttcccc acctgcgcca ttgaccacgt
    23641 tcaccacgcc cggcggcagt aaatcaccga caatttccat tagcagcagt acagaaagcg
    23701 gggtaagacg tgcgggtttc agcaccacac agttgcccgc cgccagcgcg ggagccattt
    23761 tccagctcgc catcagcagc gggaagttcc acgggataat ctgccccacc acgcctaacg
    23821 gttcatggaa atgataggcc acggtttcgc tatcaacttc actgatccca ccttcctgcg
    23881 cccgaataca cgaggcgaaa tagcggaaat ggtcaatcgc cagcggtaca tccgcagcac
    23941 tggtttcgcg aatgggtttg ccgttatccc aggtttcagc tgtcgctaac agctcgaggt
    24001 tttgttccat tcgatcggca atcttaaaca gaatcgccgc gcgatcctgc accgaggtgt
    24061 gcgcccattt atctttcact ttgtgcgcag catccagcgc cagatcgatg tctcgtttgc
    24121 ccgaagacgc cacttcgcac agcagctgcc cggtcaccgg cgtcagattc tggtaatact
    24181 caccatcggc aggggctacc cattcaccgc caataaagtt gtcgtagcgg gcttttaact
    24241 tgatggggaa accatactcg ccgggcttaa tctgtgctga agggggatta ttggtcatga
    24301 tcgtctcctt gcggtgtgag gtataacaag ggtagacgtg actggcgaaa tcttcgccag
    24361 tcggtaacag ctttacgaca gctatcacga atttacgggc aaggattaca gagatagttg
    24421 aaaaagcggc tgacaatttg ccagccgttg ttggaatgat gattacattg cggcgcgata
    24481 aatcaccaca atttcttcgt gagttgcctg gatcgggtta gtaaagccac aggcatcttt
    24541 cagggcatta gtcgccagaa ccgcgaaatc ttcttctttc acgttcaggt cgcgtaggcc
    24601 tgccgggata tccactttct tcgccagttc acggatggcg ttaatgcagg cttcagcacc
    24661 ttccgcgtcg tttttacctg tcacgttcac gcccattgca gcggcacagt cacgcagacg
    24721 tgcagcggcg actttgctgt tgaatacctg aacatgcggc agcagaacgg cgttacatac
    24781 accgtgtggc aggttgtaga aaccgcccag ctggtgcgcc atcgcatgaa cataacccag
    24841 agaagcatta ttgaacgcca taccggcaag gaactgggca taagccattg cttcacgcgc
    24901 tttcgcatta ctgccatctt caacggctaa cggcaggttt tcggcaatca tggtcacggc
    24961 tttcagtgca caagcgtcag tgatcggcgt ggcggcaata gaaacatacg cttcgatagc
    25021 gtgcgttaag gcatccatac ccgttgcggc ggtcagtgac ttcggcatac caatcatcag
    25081 agaggagtca ttgacagaaa gcagcggagt cacgtgctta tcaacaatcg ccattttgtt
    25141 atgacgcgct tcgtcagtga tgatgcagaa acgggtcatt tcagaggccg tacccgccgt
    25201 ggtattgatg gcgatcatcg gcagctgcgg ttttgcagag cggtcaacgc cttcgtaatc
    25261 gcgaatatcg ccgccattgg ctgccaccag cgcaatacct tttgcgcagt cgtgtggaga
    25321 accaccgcct aaggagatca cgctatcgca attattttct ttaagtaatt tcaaacctgc
    25381 ggcgacgttt tccgtggtgg ggttaggttg ggtgccatca taaataacgc taaaaatatt
    25441 gcgttcttcc agtgcttttt gcacatcacc cgccatacct aatttcgtta acatattgtc
    25501 agtgacaatt aaggtacggg taaatccata atctgccatc atattcattg catcagtcaa
    25561 tgaatcagcg ccgatgacat tcacagaagg aataaagaaa gttgaagatg ccataacaca
    25621 ctcctgaaag tgtaaaaaag ggtgggcagc ttactgagga ttttcattcg aaaacatgat
    25681 cattattatt aattatcaaa attaatctaa taaaaagcac attgtattca ttaaacaatg
    25741 tgctttattt aaaatcataa gaaatttgta ttaatttatt ttaaaaatta attaatcatt
    25801 tccttatttt tccggaaata ataatgcgtc gcgcaataaa tgatcatttc cacgacgacg
    25861 cgtaaagcca atgcggtcaa aatattccag aatctgaatt gccagctttc ggcctacgcc
    25921 taagcgatcg cggaaatccg ccgcgcaggt tgaaccacac tcctgatcga gatcgcggat
    25981 catattggca aattcgacaa tccgatcgtt acggtaataa cgatctttga cgatcgcggt
    26041 aattattcct tgctgcgccg cctggcgtag cgtcaggcgc attgcctgct cgtcggtacc
    26101 cgtctctttt gccagatcac gcacccacca cggctcgtca ccaaacagcg gctctgcttt
    26161 ttgccagatg gcctgctgct cttcgctgaa gcccgctttg tgatccggca gatgcagcca
    26221 accgtgatga ctgaggatgt cgccgctttc gcgcatcttt tcaatcagca acagtactag
    26281 cgcttcatct tccattggca acgccatacg tcgcagacgt tcgcgcccag gcccaggttc
    26341 atcgcgatgt tgctcatgat aagtcgctaa tgtgtcgaga attttccgtt gccagcgagc
    26401 ggcaaccggc gcattcaaca agctataacc agcctgaata tagccaggct gttgcagcag
    26461 ttcgcgcatc ccttcgccgt tgagctggcg cgcccaggcg aaatcaggaa gattaaccgc
    26521 gccgcgttgc aggtgaacgg ctaacgcttc gctatcattc tgtgccctgg cgagggaagc
    26581 cagccattgc agatactctg gtttacgttt accgcgacgc ggcgggttaa gcatcacgac
    26641 gcgcgctccg gccagcgtgt tgcgggcaga gatatcgcgc aataccaggc ggtcgttatc
    26701 tgccagccat aacggggtgt cgaagaccag ttcagcaagg ttatcttcca gcagtgaaac
    26761 gcgtcccgtg acgtggctgg cggcgtggtg aatatgcagc ggctgccact gggtcagcgg
    26821 tgcatgggtt tgaagctcga caatcacccg tgtgaacggc tctggcggca catcggcaag
    26881 cagccagtcg ccacggttaa tctgctcttt ttccgcatca cccgcgatgt caagcgcgat
    26941 acgctgcccg gcattggcgg tttctgttgg ctggttttgc gcatgcagcg cacgtacacg
    27001 catcggttta tttacaccag tcagccagag tgaatcgcct accttcactt ccccgcttaa
    27061 cgccgtaccg gtgacgacca gcccggcacc ttttacggta aatgcgcggt caatcgcgag
    27121 gcggaaacta tgttggctgg cgtgctcgcg ttccggcaac tgaagcagat gctcgcgcag
    27181 ggcatcaatt ccccgacctt cggttgctgc ggtgataaac agttttgcct cagcaaaacc
    27241 gtattcccgc agaacctcct ttacctggcg ttcaacctca tcaacacgcg cttcgtccac
    27301 gcgatcggct ttggtcagcg ccactgtcag catcgggtta ccggtcagct gcaaaatcgc
    27361 cagatgctca cgggtctgtg ccatcacgcc gtcatcgcac gccaccacca acagcgcgtg
    27421 atcgatacca ccaacgcccg ccagcatgtt ggaaagaaac ttttcatgac cgggaacgtc
    27481 gataaaacca ggcacgcgac catccggctg cggccagtag gcatagccga gatcgatggt
    27541 catgccgcgc tttttttctt ccggcagacg gtcagcattt acgccagtaa tcgcctgcaa
    27601 taaggttgtt ttgccgtggt caacgtgtcc ggcagtcgca ataatcattt caacaacatc
    27661 tccaaaaacc gttgctcatc ttcaaggcag cgtaaatcca gccacaatcg tccgtcataa
    27721 atacgaccaa tcaccggcac tggcaattca cgccagcggg cggctaatga ctcaaggtgg
    27781 ctaccgcgtc catcatgggg tgtaaacgtt aatgccgcgc tcggcaggcg atcaaccggc
    27841 agcgaaccac tgccaatctg cgaaagacat ggcataacct gtaccgcaaa ctccgcgccg
    27901 taatgtgcga caaggggagc ctgtaaacgt tgtgcctgga tttgaatgac ctctgcgctg
    27961 cgggtaagca ggcgcagggt cggtaatttt tcactcagag cttcagggtg taaataaaga
    28021 cgcaacgtgg cttccagcgc cgcgagggtc attttatccg cgcgtaatgc acgcttcagc
    28081 gggtggcttt gcaggcgggc gatcatctct tttttaccaa cgataatccc ggcctgcggc
    28141 ccgcctaaca acttgtcgcc ggagaagctc accagactga cgcccgccgc aatcaactcc
    28201 tgcggcattg gctctttcgg caaaccgtac tggctaagat cgaccagcga gccactgcct
    28261 aaatcagtca ccactggaac atccagctct ttgccgagcg ccaccagttc agcttcgtct
    28321 atcgctttgg tgaacccctg aatgctgtag ttactggtat gtactttcat caacagtgcg
    28381 gtattttcat tcaccgcctg acgataatca ttcgcgtgcg tgcggttggt ggtccctact
    28441 tcgtgtaggg tgcagcctgc ctgacgcata acatcgggaa tacgaaacgc gccgccaatc
    28501 tccaccagtt cgccgcgaga taccaccacc tctttcccgc tggcagtggc cgccaacatc
    28561 aataacaccg ccgccgcatt gttattgacg atacaggcat cttccgcccc cgtaatacgg
    28621 cacagcagct gcgccagcgc ccgatcgcga tgtccgcgtc cggcgtcgtc cagatcgtac
    28681 tcgagggtca ctggcgaacg catagcctgc gcaacggctt ccaccgcggc ttccgcctgt
    28741 aaagctcgcc caaggttggt atgcagcacg gttcccgtca ggttgatcac cggacgcagc
    28801 gcgctctgcg cctctttcgt caaccgggca tcgacttctt gcgcccagtt ttcacaccac
    28861 gcaggcagcg tctggctgcc acgaatcact tctcgcgctt cgtcgagcat ctgacgcaac
    28921 aattccacca cgcgggtgtg accataagta tcacgcaaag aaaggaagga gctatcgcgc
    28981 aataagcgat caatagccgg aagttgacta tagagggaac gcgtttcggt tgtcatagga
    29041 aacctggctg atcaaggccc tctcacacgg agaagggcgt ttaacataac cacggattgt
    29101 aacgtgagat gggtcaggag gacatatcgc gcatcaagcc tttggcggtt cggtgcgagc
    29161 aaaactttcg cggctaaaca ggttttcgac cagtttgact aagtgtgggc ggtcgacaca
    29221 ccaacctggc gcaacgcggc ggaaattgag atatccgaca gcacaggcaa tggcgatagt
    29281 cgccagattg accgtatcgg ttttgagtgt gccatcgacc agatatcctt ccagcacatc
    29341 cagactgcgg ttgattttct cccgctggcg taacaattca tcttcagact gctgcgccgc
    29401 cggacgcgcc tgttcacgca ccgataccag cccggcatcc ataatgccat ccgccagcgc
    29461 ctcaattttg cgcacccgca acgactccag cggatcgcgc ggcaacatcg ccggagcgac
    29521 attcattaac tcaatatatt cagcgatgat cggcgaatca aaccagcatt cgccctcttc
    29581 ggtcaccagc accggcactt tccctaacgg gttaaattgc gccacgccgt tatccgcgtt
    29641 atagggcagt tcattaataa attcgaaagt tatgcccttt tctaacaaca gaatagaaag
    29701 tttgcgtaca aacgggctgg tgtagctacc gacgagtttc atgccgagtc ctttgtgcga
    29761 ggaaaaatat cagtatggct caggctcacg caaaacacag gtgatataag tctcaatgat
    29821 ccaaataaag ataatgcatc caactggtca ttcttagcag cactttccgc atcaccgaaa
    29881 catggctgaa atggtcggag taaaccgcca cctgggcgta gatgccgtcg ggtaaggcat
    29941 cgatatcatc gttagggtcc agttcaatgg ttcccagcac accgtccgtg ccgggaacga
    30001 ccgttaatga ttgcaatacc ccctgcgcct gataagaacc gcctggcacg acaggtaaaa
    30061 tactagtcaa tttgccgtgg aacacctgcc caggtagcgc gttaaacacc acttccgcat
    30121 catcgccagg tttcagacgt aacagcgagt tttgccgaaa ttgggcgaca atttgccgtt
    30181 tttgctcggg gatgaagacc atcaccggac gcagcggcaa ggcagctgcg tatgtacctg
    30241 ggcggatcag tacctgagtg acgtagccat tgcttggtgc gcgaatgaca gtctgctcaa
    30301 gattatattt tgcttcagta agttgcgctc ttaagctcac aatctgcgat tgctcgccgt
    30361 tgaccatact gtcgagctga ctctggatct gcgcctgctc cgccaccgag cctttcacta
    30421 acgcatcctg cgcgaggaaa ttttgccgcg catcgtcgat gtcacgttcc gagaacgaat
    30481 tcaccgccgc ctggctgcct ttcaggtaac gttgataatt tttaaacaga cggtcgcgct
    30541 ccgctgaaac ctgggtggtg ttggcctgcg cttcagtgag ctgcgcacgc agcgtcttta
    30601 tattatgcgt cgccgtcatc aggtcagcct gaagtctgtc aactcgcgcc tggtaacgaa
    30661 ccgggtcgag cttaaaaagc acctcgccct tttgaataag ctgattattc ttgtcagtga
    30721 cttcagtaac aattcccgtc acctgtggcg tgatagggat cgctatcact gccttttgcg
    30781 cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat caaaccactc accagaaaca
    30841 cgcctcccag cgccgccgtc gccagcgcca cgtaagttaa aacaatcaat agatccataa
    30901 ttagatctcc gggaacgtgg aatagtcagt ctttttctcg gcggcgagtt gatgctccag
    30961 ccgggaaatt cggtcagaaa gtgcggcgat ttccggttca gtcgctttct cctgcgacgc
    31021 aacatgtgac tgcatacccc agccacgctc cggttgatag agcgtcgccc agatccacag
    31081 aaacggccag ataacatgca gagtaaacag gctcacccag cccgccgtat gaatagcgtc
    31141 ggcatggggg tgattgcgct ttttggcaat cagataaggg atgtcatgta tcgcgatgat
    31201 tccataaaaa atcaccagaa agacgaagat cagcactccc aacgcgaaat agtttagaaa
    31261 catatctgcc tcggattcac gtttatcagt gttgtttttt ggctggcagc cagaagggag
    31321 tcaggctgat attttgacaa taatccgggt tcgcgattct cgccataaca ccaaagaata
    31381 atttttagag gtgatgagtt gcttagttac ataacgattg tatgacgaag gcataacatg
    31441 ctgtagatca catcaggtga acgccgtaag aaaatatctt gtgattcaga tcacaaagat
    31501 tcaacaaacc atcaaaacaa aaatgtgaca ttactcacat ttaaatgcca tttttagcga
    31561 aaatcaccgc cttgtttctt ttttatacag gcgttttgtg atgagcgtca cgtcaattac
    31621 ctctctaccc cctatattta tgtgactgat atcacacaaa agcccgtcga ctggacagtt
    31681 aaccgattca gtgccagatt tcgcagtatc tacaaggtcc ggctacctct gccgccacat
    31741 taacaaaaaa cctcgggctt ccagcctgcg cgacagcaaa cataagaagg ggtgttttta
    31801 tgtcatccga tattaagatc aaagtgcaaa gctttggtcg tttcctcagc aacatggtga
    31861 tgccaaatat cggcgcgttt atcgcgtggg gtatcatcac cgcgttattt attccaacag
    31921 ggtggttacc gaacgagacg ctggcgaagc tggtcgggcc gatgatcact tatctcctac
    31981 cgctgctgat cggttatacc ggtggtaagc tggtaggcgg caaacgtggt ggcgtagtcg
    32041 gtgccattac caccatgggc gttatcgtcg gcgcagacat gccgatgttc ctcggttcta
    32101 tgattgcagg tccgctgggc ggctggtgca ttaagcactt cgaccgctgg gtagacggta
    32161 agatcaaatc cggttttgag atgctggtga ataacttctc cgcaggcatc atcgggatga
    32221 tcctcgctat tctggcattc ctcggcattg gcccgattgt tgaagccctg tccaaaatgc
    32281 tggctgcggg cgttaacttc atggttgtcc atgacatgct gccgctggcg tctatctttg
    32341 ttgaaccggc gaaaatcctg ttcctcaaca acgccattaa ccacggtatc ttctcgccgc
    32401 tgggtattca gcagtcccat gaactgggta aatcaatctt cttcctgatt gaagctaacc
    32461 caggtccagg tatgggcgtg ctgctggcgt acatgttctt tggtcgtggt agcgctaaac
    32521 agtctgcggg cggtgcggca atcatccact tcctgggtgg tatccacgaa atctacttcc
    32581 cgtatgtgct gatgaatccg cgtctgatcc tcgcagtcat cctcggcggt atgactggcg
    32641 tgttcacgct gactatcctg ggcggtggtc tggtttctcc ggcatctccg ggttctatcc
    32701 ttgctgtact ggcgatgaca ccaaaaggtg cttatttcgc taacatcgcg ggtgtgtgtg
    32761 cggcgatggc tgtctccttc gttgtctctg ctattttgct gaaaaccagc aaagtgaaag
    32821 aagaagatga tattgaagca gcaactcgtc gtatgcagga catgaaagct gagtctaaag
    32881 gcacatctcc gctatctgcg ggcgatgtga ctaacgacct gagccacgta cgtaaaatca
    32941 tcgttgcctg tgacgccggt atgggttcaa gtgcgatggg cgcaggcgtg ctgcgtaaga
    33001 aaattcagga tgcaggtctg tcgcagattt cggtcactaa cagcgcgatc aacaacctgc
    33061 cgccagatgt ggacctcgtc atcactcacc gtgacctgac cgaacgcgct atgcgccagg
    33121 ttccgcaggc acagcatatt tcgctgacca acttcctcga cagcggcctg tacaccagcc
    33181 taaccgaacg tctcgttgct gcccagcgcc atactgaaaa cgaagtgaaa gtgaaagaca
    33241 gtctgaaaga cagctttgac gattccagtg ctaacctgtt caaactgggc gcggagaaca
    33301 tcttcctcgg tcgcaaagca gcaaccaaag aagaagcgat tcgttttgct ggcgagcagc
    33361 tggtgaaagg cggttacgtt gagccggaat acgttcaggc gatgctggat cgtgaaaaac
    33421 tgaccccgac ttatctgggt gagtctatcg cggtgccaca cggtacggtt gaagcgaaag
    33481 atcgcgtact gaaaacgggc gtggtgttct gccagtaccc ggaaggcgtg cgcttcggtg
    33541 aagaagaaga tgacattgcc cgtctggtga ttggtattgc agcccgtaac aacgagcaca
    33601 ttcaggttat caccagcctg accaatgcac tggatgatga gtccgtcatc gagcgtctgg
    33661 cacacaccac cagcgtggat gaagtgctgg aactgctggc aggtcgtaag taatccaatc
    33721 ctaccctctc ctcacggaga gggtttgggt gagggaaaag cctcacccca gccctctcgg
    33781 gtaaaaacat tgatgaaggt taatactatg aaagcattac attttggcgc aggtaatatc
    33841 ggtcgtggct ttatcggtaa actgctggca gacgcgggta tccaactgac gtttgccgat
    33901 gtcaatcagg tggtacttga tgccctgaat gcccgtcata gctatcaggt tcatgtggtc
    33961 ggtgaaaccg agcaggtaga taccgtttcc ggcgtcaatg ctgtcagcag cattggtgat
    34021 gatgtcgttg atctgattgc tcaggtcgat ttagtcacta ccgccgttgg cccggttgtg
    34081 ctggaacgta ttgctccggc tatcgccaaa gggctggtga aacgcaaaga acaaggtaat
    34141 gaatccccgc tgaacatcat cgcctgtgaa aacatggtac gcggcaccac gcagctgaaa
    34201 ggtcatgtga tgaacgccct gccggaagac gccaaagcgt gggtagaaga gcacgttggc
    34261 tttgtcgatt ccgccgttga ccgcatcgta ccgccttcgg cttcggcaac taacgatccg
    34321 ctggaagtga cggtagaaac tttcagcgaa tggattgtcg ataaaacgca gttcaaaggc
    34381 gcactgccga acatcccagg catggagtta accgacaacc tgatggcatt tgtcgaacgt
    34441 aaactcttca ccctgaacac gggtcatgct ataaccgcgt acctcggaaa actggccggt
    34501 catcagacca ttcgtgacgc tattctcgac gagaaaatcc gcgcggtggt aaaaggtgcg
    34561 atggaagaaa gtggtgcggt actgatcaag cgctacggct ttgacgcaga caaacatgcg
    34621 gcgtacatcc agaaaatcct cggtcgtttt gagaacccgt atctgaaaga tgatgtagag
    34681 cgcgtaggcc gtcagccgct gcgtaaactg agtgctggcg accgtctgat caagccactg
    34741 ctcggtacgc tggaatacag ccttccgcac aagaatctga ttcaggggat tgctggtgca
    34801 atgcacttcc gcagtgaaga tgatccgcag gctcaggaac tggcagcact gatcgctgac
    34861 aaaggtccgc aggcggcgct ggcacagatt tccggtcttg atgccaacag cgaggttgta
    34921 tccgaggcgg taaccgctta taaagcaatg caataatggt ggaccaggcg caggacaccc
    34981 tgcgcccgaa taacagattg tcagatatgc aggcaacaat ggaacaaacc caggccgttg
    35041 aaaaccgtgt gcttgagcgt ctgaatgctg gcaaaaccgt gcgaagcttt ctgatcaccg
    35101 ccgtcgagct tttgaccgag gcggtaaatc ttctggtgct tcaggtattc cgcaaagacg
    35161 attacgcggt gaagtatgct gtagaaccgt tactcgacgg cgacggtccg ctgggcgatc
    35221 tttctgtgcg tttaaaactc atttacgggt tgggcgtcat taaccgccag gaatacgaag
    35281 atgcggaact gctgatggca ttgcgtgaag agctaaatca cgacggcaac gagtacgcct
    35341 ttaccgacga cgaaatcctt ggaccctttg gtgaactgca ctgcgtggcg gcgttaccac
    35401 cgccgccaca gtttgaacca gcagactcca gtttgtatgc aatgcaaatt cagcgctatc
    35461 aacaggctgt gcgatcaaca atggtccttt cactgactga gctgatttcc aaaatcagct
    35521 taaaaaaagc ctttcaaaag taagcaacgt ctgcttactg cccctctacc tgcttcggcc
    35581 gataaagccg acgataatac tccagacgtt gtagatataa cggcacgctt tcatcgggta
    35641 ttccggacgg aatcgcgtta cggggaggaa gttttttcag atactcccgg aacgcctggc
    35701 ttgatgccat gaaatctacg gctttatcga taagaagcgg aacgttttca cctaatttgc
    35761 ccatgatgat atctccgtat taccccgcgc cgggaatgcg cggcctccaa ttttagttta
    35821 ggttcccggt aaagatgaca ttaaggaaaa cgtgctgaat cctcaataat catccgctgc
    35881 tgtttattat ctttttctat agatttctta attaatcaac gaattgcgta gtttttgagc
    35941 atgttaatga ttacgttgca gaactattac aatgtcatca gttgtgaaca aagcacctgg
    36001 tcgcgcatac taggggctat aaatttatct ttatcagaag ccatcacatg aaagaagtcg
    36061 aaaaaaacga aatcaagcgt ctcagcgatc gcctggacgc catccgccac cagcaggccg
    36121 atctgtcgct ggttgaagcc gcagacaaat atgccgagct ggaaaaagag aaagccacgc
    36181 tggaagcaga aattgctcgc ctgcgtgagg ttcatagcca aaaactgagt aaagaagcac
    36241 agaagctgat gaagatgccg ttccagcgcg caattaccaa aaaagagcag gctgatatgg
    36301 gcaagctgaa gaaaagtgtt cgcggactgg tggttgtgca cccaatgacc gcgctggggc
    36361 gcgaaatggg cctggaagag atgactggat tttctaagac tacgttttaa gcccctcaga
    36421 cgactggttc gatcgtatca acccgcatct ggcttgcctt agctggtcaa tgcggggact
    36481 ctcctgtcta ctcgcctaat taccaaacaa ttcactccct ttacttcgat ttaccgctgg
    36541 ttgatattct gaaaatgcat ttttaccgaa atagccatct a
//
LOCUS       DAECNO010000032        36520 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.032, whole genome shotgun sequence.
ACCESSION   DAECNO010000032 DAECNO010000000
VERSION     DAECNO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.032"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(156..1391)
                     /locus_tag="KKO08_001641"
     CDS             complement(156..1391)
                     /locus_tag="KKO08_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD6317929.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="KKO08_001642"
     tRNA            complement(1538..1614)
                     /locus_tag="KKO08_001642"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="KKO08_001643"
     tRNA            complement(1657..1743)
                     /locus_tag="KKO08_001643"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="KKO08_001644"
     tRNA            complement(1764..1839)
                     /locus_tag="KKO08_001644"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="KKO08_001645"
     tRNA            complement(1898..1974)
                     /locus_tag="KKO08_001645"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="KKO08_001646"
     CDS             complement(2077..3462)
                     /locus_tag="KKO08_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6317930.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KKO08_001647"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="KKO08_001647"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBD6317931.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KKO08_001648"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="KKO08_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBD6317932.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KKO08_001649"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="KKO08_001649"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBD6317933.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KKO08_001650"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="KKO08_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBD6317934.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KKO08_001651"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="KKO08_001651"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBD6317935.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KKO08_001652"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="KKO08_001652"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBD6317936.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KKO08_001653"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="KKO08_001653"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD6317937.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KKO08_001654"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="KKO08_001654"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD6317938.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KKO08_001655"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="KKO08_001655"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBD6317939.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KKO08_001656"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="KKO08_001656"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBD6317940.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KKO08_001657"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="KKO08_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBD6317941.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KKO08_001658"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="KKO08_001658"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBD6317942.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KKO08_001659"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="KKO08_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBD6317943.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KKO08_001660"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="KKO08_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBD6317944.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KKO08_001661"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="KKO08_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBD6317945.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="KKO08_001662"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="KKO08_001662"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBD6317946.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="KKO08_001663"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="KKO08_001663"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBD6317947.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KKO08_001664"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="KKO08_001664"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBD6317948.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="KKO08_001665"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="KKO08_001665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBD6317949.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KKO08_001666"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="KKO08_001666"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBD6317950.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="KKO08_001667"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="KKO08_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBD6317951.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KKO08_001668"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="KKO08_001668"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBD6317952.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KKO08_001669"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="KKO08_001669"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBD6317953.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KKO08_001670"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="KKO08_001670"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBD6317954.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KKO08_001671"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="KKO08_001671"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBD6317955.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="KKO08_001672"
     CDS             complement(31267..32913)
                     /locus_tag="KKO08_001672"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBD6317956.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="KKO08_001673"
     CDS             complement(32916..32966)
                     /locus_tag="KKO08_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6317957.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KKO08_001674"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="KKO08_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBD6317958.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="KKO08_001675"
     CDS             33502..35022
                     /locus_tag="KKO08_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBD6317959.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KKO08_001676"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="KKO08_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBD6317960.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="KKO08_001677"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="KKO08_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBD6317961.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="KKO08_001678"
     tRNA            complement(36439..36514)
                     /locus_tag="KKO08_001678"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8918 a         9996 c         9200 g         8406 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg tactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcatat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgtacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg gaatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acattaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAECNO010000033        35155 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.033, whole genome shotgun sequence.
ACCESSION   DAECNO010000033 DAECNO010000000
VERSION     DAECNO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.033"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            88..1116
                     /gene="murB"
                     /locus_tag="KKO08_001679"
     CDS             88..1116
                     /gene="murB"
                     /locus_tag="KKO08_001679"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBD6317962.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVCFIGASGE
                     VSAVETIS"
     gene            1113..2078
                     /gene="birA"
                     /locus_tag="KKO08_001680"
     CDS             1113..2078
                     /gene="birA"
                     /locus_tag="KKO08_001680"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBD6317963.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="KKO08_001681"
                     /gene_synonym="panK"
     CDS             complement(2107..3057)
                     /gene="coaA"
                     /locus_tag="KKO08_001681"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBD6317964.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            3419..3494
                     /locus_tag="KKO08_001682"
     tRNA            3419..3494
                     /locus_tag="KKO08_001682"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3452..3454,aa:Thr,seq:tgt)
     gene            3503..3587
                     /locus_tag="KKO08_001683"
     tRNA            3503..3587
                     /locus_tag="KKO08_001683"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3537..3539,aa:Tyr,seq:gta)
     gene            3704..3778
                     /locus_tag="KKO08_001684"
     tRNA            3704..3778
                     /locus_tag="KKO08_001684"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3737..3739,aa:Gly,seq:tcc)
     gene            3785..3860
                     /locus_tag="KKO08_001685"
     tRNA            3785..3860
                     /locus_tag="KKO08_001685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3818..3820,aa:Thr,seq:ggt)
     gene            3975..5159
                     /gene="tuf"
                     /locus_tag="KKO08_001686"
     CDS             3975..5159
                     /gene="tuf"
                     /locus_tag="KKO08_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBD6317965.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            5389..5772
                     /gene="secE"
                     /locus_tag="KKO08_001687"
     CDS             5389..5772
                     /gene="secE"
                     /locus_tag="KKO08_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBD6317966.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            5774..6319
                     /gene="nusG"
                     /locus_tag="KKO08_001688"
     CDS             5774..6319
                     /gene="nusG"
                     /locus_tag="KKO08_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBD6317967.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            6478..6906
                     /gene="rplK"
                     /locus_tag="KKO08_001689"
     CDS             6478..6906
                     /gene="rplK"
                     /locus_tag="KKO08_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBD6317968.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            6910..7614
                     /gene="rplA"
                     /locus_tag="KKO08_001690"
     CDS             6910..7614
                     /gene="rplA"
                     /locus_tag="KKO08_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBD6317969.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            8027..8524
                     /gene="rplJ"
                     /locus_tag="KKO08_001691"
     CDS             8027..8524
                     /gene="rplJ"
                     /locus_tag="KKO08_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBD6317970.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            8591..8956
                     /gene="rplL"
                     /locus_tag="KKO08_001692"
     CDS             8591..8956
                     /gene="rplL"
                     /locus_tag="KKO08_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBD6317971.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            9276..13304
                     /gene="rpoB"
                     /locus_tag="KKO08_001693"
     CDS             9276..13304
                     /gene="rpoB"
                     /locus_tag="KKO08_001693"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBD6317972.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            13381..17604
                     /gene="rpoC"
                     /locus_tag="KKO08_001694"
     CDS             13381..17604
                     /gene="rpoC"
                     /locus_tag="KKO08_001694"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBD6317973.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="KKO08_001695"
     CDS             complement(17846..18979)
                     /gene="thiH"
                     /locus_tag="KKO08_001695"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBD6317974.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="KKO08_001696"
     CDS             complement(18976..19746)
                     /gene="thiG"
                     /locus_tag="KKO08_001696"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBD6317975.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="KKO08_001697"
     CDS             complement(19748..19948)
                     /gene="thiS"
                     /locus_tag="KKO08_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBD6317976.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="KKO08_001698"
     CDS             complement(19932..20687)
                     /gene="thiF"
                     /locus_tag="KKO08_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBD6317977.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="KKO08_001699"
     CDS             complement(20680..21315)
                     /gene="thiE"
                     /locus_tag="KKO08_001699"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBD6317978.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="KKO08_001700"
     CDS             complement(21315..23210)
                     /gene="thiC"
                     /locus_tag="KKO08_001700"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBD6317979.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(23291..23391)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="KKO08_001701"
     CDS             complement(23444..23920)
                     /gene="rsd"
                     /locus_tag="KKO08_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBD6317980.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            24015..24788
                     /gene="nudC"
                     /locus_tag="KKO08_001702"
     CDS             24015..24788
                     /gene="nudC"
                     /locus_tag="KKO08_001702"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBD6317981.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            24828..25892
                     /gene="hemE"
                     /locus_tag="KKO08_001703"
     CDS             24828..25892
                     /gene="hemE"
                     /locus_tag="KKO08_001703"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBD6317982.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            26049..26720
                     /gene="nfi"
                     /locus_tag="KKO08_001704"
     CDS             26049..26720
                     /gene="nfi"
                     /locus_tag="KKO08_001704"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBD6317983.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            26763..27353
                     /locus_tag="KKO08_001705"
     CDS             26763..27353
                     /locus_tag="KKO08_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBD6317984.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            27540..27812
                     /gene="hupA"
                     /locus_tag="KKO08_001706"
     CDS             27540..27812
                     /gene="hupA"
                     /locus_tag="KKO08_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBD6317985.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            27825..28520
                     /locus_tag="KKO08_001707"
     CDS             27825..28520
                     /locus_tag="KKO08_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBD6317986.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="KKO08_001708"
     CDS             complement(28522..28947)
                     /gene="zraP"
                     /locus_tag="KKO08_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBD6317987.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            29185..30561
                     /gene="zraS"
                     /locus_tag="KKO08_001709"
     CDS             29185..30561
                     /gene="zraS"
                     /locus_tag="KKO08_001709"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBD6317988.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            30558..31883
                     /gene="zraR"
                     /locus_tag="KKO08_001710"
     CDS             30558..31883
                     /gene="zraR"
                     /locus_tag="KKO08_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBD6317989.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(31880..33169)
                     /gene="purD"
                     /locus_tag="KKO08_001711"
     CDS             complement(31880..33169)
                     /gene="purD"
                     /locus_tag="KKO08_001711"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBD6317990.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(33181..34770)
                     /gene="purH"
                     /locus_tag="KKO08_001712"
     CDS             complement(33181..34770)
                     /gene="purH"
                     /locus_tag="KKO08_001712"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBD6317991.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         8383 a         8997 c         9572 g         8203 t
ORIGIN      
        1 ctacaaactc ttcctgtcgt catatctaca agccatcccc ccacagatac ggtaaactag
       61 cctcgttttt gcatcaggaa agcagctatg aaccactcct taaaaccctg gaacacattt
      121 ggcattgatc ataatgctca gcacattgta tgtgccgaag acgaacaaca attactcaat
      181 gcctggcagc atgcaaccgc agaaggacaa cccgttctta ttctgggtga aggaagtaat
      241 gtactttttc tggaagacta tcgcggcacg gtgatcatca accggatcaa aggtatcgaa
      301 attcatgatg aacctgatgc gtggtattta catgtaggag ccggagaaaa ctggcatcgc
      361 ctggtaaaat acactttgca ggaaggtatg cctggtctgg aaaatctggc attaattcct
      421 ggttgtgtcg gctcatcacc tatccagaat attggtgctt atggcgtaga attacagcga
      481 gtttgcgctt atgttgattg tgttgaactg gcgacaggca agcaagtgcg cttaactgcc
      541 aaagagtgcc gttttggcta tcgcgacagt atttttaaac atgaatacca ggatcgcttc
      601 gccattgtag ccgtaggtct gcgtctgcca aaagagtggc aacctgtact aacgtatggt
      661 gacttaactc gtctggatcc tacaacagta acgccacagc aagtatttaa tgcggtgtgt
      721 catatgcgca ccaccaaact ccctgatcca aaagtgaatg gcaatgccgg tagtttcttc
      781 aaaaaccctg ttgtatctgc cgaaacggct aaagcattac tggcgcaatt tccaacagca
      841 ccaaattacc cccaggcggg tggttcagta aaactggcag caggttggct tattgatcag
      901 tgccagctaa aagggatgca aatgggtggg gctgcggtgc accgtcaaca ggcgttagtt
      961 ctcattaatg aagacaatgc aaaaagcgaa gatgtggtgc aactggcaca ccatgtaaga
     1021 caaaaagtgg gtgaaaaatt taatgtctgg cttgagcctg aagtctgctt tattggtgca
     1081 tcaggtgaag tgagcgcagt ggagacaatt tcatgaagga taacaccgtg ccactgaaat
     1141 tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt gaaatgctgg
     1201 gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg ggcgttgatg
     1261 tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc catccagtta cttaatgctg
     1321 aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtt attgactcca
     1381 cgaatcagtt ccttcttgat cgtatcggag agcttaaatc gggcgatgcc tgtgttgcag
     1441 aataccagca ggctggccgt ggtcgccggg ggcggaaatg gttttcgcct tttggcgcaa
     1501 acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg attggtttaa
     1561 gtctggttat cggtatcgtg atggcggaag tattacgaaa gctgggagca gataaagttc
     1621 gtgtcaaatg gcctaatgac ctctatctgc aggatcgcaa gctggcaggc attcttgtgg
     1681 agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg atcaacatgg
     1741 caatgcgccg tgttgaagag agtgtcgtta atcaggggtg gatcacgctg caggaagcgg
     1801 ggatcaatct tgatcgtaat acgttggcgg ccatgctaat acgtgaatta cgtgctgcgt
     1861 tggaactctt cgaacaagaa ggattggcac cttatctgtc gcgctgggaa aagctggata
     1921 attttattaa tcgcccagtg aaacttatca ttggtgataa agaaatattt ggcatttcac
     1981 gcggaataga caaacagggg gctttattac ttgagcagga tggaataata aaaccctgga
     2041 tgggcggtga aatatccctg cgtagtgcag aaaaataaga aaggggagta ttcgctcccc
     2101 tgcaaattat ttgcgtagtc tgacctcttc taccacatga ttagcacttt tcgtcaggat
     2161 taaactggcg cgctcacgag taggtagaat attttgcttt aagttcagcc agttgatctc
     2221 tttccacaat gtcatggcag tcttaatcgc ttcttcttta gttaatttcg cgtagttatg
     2281 aaaataggaa tccgggtctg taaaagcccc ttcgcggaat ttcagaaaac ggttgatata
     2341 ccacgtctga agtaagtctt ccggtgcatc aacatatatc gaaaaatcga caaaatcgga
     2401 aacaaataca tgatgtggat cgtgtggata atccatcccg ctctgtaaga catttaaccc
     2461 ttcaagaatt aaaatatcag gctgaacaac agttttatct ccatccggga tcacatcata
     2521 aataagatgc gagtaaacag gggctgtaac gtttggcacg ccggatttga gatcggaaac
     2581 aaacttcacc aggcgatgca tatcatacga ttccgggaag cctttcttct tcatcagacc
     2641 acgttctttc agaacctgat tagggtgaag gaagccatct gtagtgatca gttcaacacg
     2701 acgatgttcc ggccaacggc ttaatagcgc ctgcaataca cgggcggttg tacttttccc
     2761 caccgcgaca ctgccagcaa tactgataat gtaaggaatg cgttgcccgt tggtaccaag
     2821 aaactgttcc agaactgcct gacggcgcag attcgagctt atatagaagt tcagcaaacg
     2881 tgacaaaggt aaatagatct cggcaacttc ttctaacgag agatcttcat taataccttt
     2941 gagacgggcg atctcatctt ccgataacgt cataggtacg gaatcacgca gagctgccca
     3001 ctggttgcgg tcaaactgta ggtaaggcgt cattaacgtt tgctctttta tactcataag
     3061 catgtttctg gcggtcatag ctctatcggc aaagcgatat ttgccatggc gcgtcacagt
     3121 tctgttgaat gggttaatgg gcaggagggt aacaccagat ggggaggaat aataagaaaa
     3181 aatctctgcc aggaagctat cgttgaaaag caatgtgaca gatattccat ctgataaaaa
     3241 gaattatggt ttagcaggag cgcattgttg agcacaatga tgttgaaaaa gtgtgctaat
     3301 ctgccctccg ttcggctgtt tcttcatcgt gtagcataaa atgtgaccaa taaaacaaat
     3361 tatgcaattt tttagttgca tgaactcgca tgtctccata gaatgcgcgc tacttgatgc
     3421 cgacttagct cagtaggtag agcaactgac ttgtaatcag taggtcacca gttcgattcc
     3481 ggtagtcggc accatcaagt ccggtggggt tcccgagcgg ccaaagggag cagactgtaa
     3541 atctgccgtc acagacttcg aaggttcgaa tccttccccc accaccaatt tcggccacgc
     3601 gatggcgtag cccgagacga taagttcact taccggctcg aataaagaga gcttctctcg
     3661 atattcagtg cagaatgaaa atcaggtagc cgagttctag gatgcgggca tcgtataatg
     3721 gctattacct cagccttcca agctgatgat gcgggttcga ttcccgctgc ccgctccaag
     3781 atgtgctgat atagctcagt tggtagagcg cacccttggt aagggtgagg tcggcagttc
     3841 gaatctgcct atcagcacca cttcttttct cctccctgtt ttttccttct gtttattgca
     3901 ttcaacaagt cgggcatgtt gcctggttga tgtggtgata tcaccgattt atccgtgtct
     3961 tagagggaca atcgatgtct aaagaaaagt ttgaacgtac aaaaccgcac gttaacgtcg
     4021 gtactatcgg ccacgttgac catggtaaaa caacgctgac cgctgcaatc actaccgtac
     4081 tggctaaaac ctacggcggt gctgctcgcg cattcgacca gatcgataac gcgccggaag
     4141 aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc ccgacccgtc
     4201 actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg atcaccggtg
     4261 ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg atgccgcaga
     4321 ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc gtgttcctga
     4381 acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg gaagttcgtg
     4441 aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt ggttctgctc
     4501 tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaactg gctggcttcc
     4561 tggattctta cattccggaa ccagagcgtg cgattgacaa gccgttcctg ctgccgatcg
     4621 aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta gaacgcggca
     4681 tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag aagtctacct
     4741 gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg gcgtgctggt gagaacgtag
     4801 gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc
     4861 cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg tccaaagatg
     4921 aaggcggtcg tcatactccg ttcttcaaag gctaccgtcc gcagttctac ttccgtacta
     4981 ctgacgtgac tggtaccatc gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca
     5041 acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg
     5101 caatccgtga aggcggccgt accgttggcg cgggcgttgt agcaaaagtt ctgagctaat
     5161 tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc ctttttgcac
     5221 gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat tgctgagaca
     5281 ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg
     5341 cgatcgcggg gtgaaaatgt ttgtagaaaa cttctgacag gttggtttat gagtgcgaat
     5401 accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca
     5461 ttgctcctgg cggcgattgt cggcaactac ctttatcgcg acattatgct gccgctgcgt
     5521 gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa
     5581 ggtaaagcta ccattgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg
     5641 ccgattcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg
     5701 tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt tatcactggc
     5761 ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc
     5821 cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac acaacatgga
     5881 agatttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca
     5941 gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga tggtgatgaa
     6001 cgacgcgagc tggcacttgg tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac
     6061 ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga accgcctgca
     6121 gcaggttggt gataagccgc gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt
     6181 taatgatggt ccgttcgctg acttcaacgg tgttgttgaa gaagtggatt acgagaaatc
     6241 tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag
     6301 ccaggttgaa aaagcctaac ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg
     6361 cgtgagattg gaatacaatt tcgcgccttt tgtttttatg ggccttgccc gtaaaacgat
     6421 tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga atttataatg
     6481 gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc taacccgagt
     6541 ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg
     6601 ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt
     6661 tacgctgacc gttctttcac tttcgttacc aagaccccgc cggcagcagt tctgctgaaa
     6721 aaagcggcag gtatcaagtc tggttccggt aagccgaaca aagacaaagt gggtaaaatt
     6781 tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac tggtgccgac
     6841 attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct ggtagtggag
     6901 gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgtgagaa agttgatgca
     6961 accaaacagt acgacatcaa cgaagctatc gcactgctga aagagctggc gactgctaaa
     7021 ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa atctgaccag
     7081 aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg cgtagccgta
     7141 tttacccaag gtgcaaacgc tgaagctgct aaagctgcag gcgcagaact ggtaggtatg
     7201 gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt tattgcttct
     7261 ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg tggcctgatg
     7321 ccaaacccga aagtgggtac tgtaacaccg aacgttgctg aagcggttaa aaacgctaaa
     7381 gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac catcggtaaa
     7441 gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt tgcgctgaaa
     7501 aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat ctccaccacc
     7561 atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt
     7621 tacgtgggcg gtgattttgt ctacaatctt acccccacgt ataatgctta atgcagacgt
     7681 atatccgaga tattcgggtt gtggcaaggc ggcaactgag tgagtcgcca agagcatagc
     7741 taactatgtg actggtgcga atgaaggaag ccaacgccgc cacaagttga atagcgacgg
     7801 atagaaaaga tttgttcgtt ggagcctggc ctatccaggc ctccgtcgaa gaccgcagga
     7861 gtttcgcaag aaacttaatc ccctgcgtag acggtgacag aacgctaaga ttattctttt
     7921 atattctggc ttgtttctgc tcaccgtaat taagacgctc tctccgtttg gaggagtgaa
     7981 gtgagttcca gagattttct ctggcaaaca tccaggagca aagctaatgg ctttaaatct
     8041 tcaagacaaa caagcgattg ttgctgaagt cagcgaagta gccaaaggcg cgctgtctgc
     8101 agtagttgcg gattcccgtg gcgtaactgt agataaaatg actgaactgc gtaaagcagg
     8161 tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc ctgctgcgcc gtgctgttga
     8221 aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt ccgaccctga ttgcatactc
     8281 tatggaacac ccgggcgctg ctgctcgtct gttcaaagag ttcgcgaaag cgaatgcaaa
     8341 atttgaggtc aaagccgctg cctttgaagg tgagctgatc ccggcgtctc agatcgaccg
     8401 cctggcaact ctgccgacct acgaagaagc aattgcacgc ctgatggcaa ccatgaaaga
     8461 agcttcggct ggcaaactgg ttcgtactct ggctgctgta cgcgatgcga aagaagctgc
     8521 ttaatcgcag ttatcttttt aacgcattcg cttacgtata aacttattct gatattcagg
     8581 aacaatttaa atgtctatca ctaaagatca aatcattgaa gcagttgcag ctatgtctgt
     8641 aatggacgtt gtagaactga tctctgcaat ggaagaaaaa ttcggtgttt ccgctgctgc
     8701 tgctgtagct gtagctgctg gcccggttga agctgctgaa gaaaaaactg aattcgacgt
     8761 aattctgaaa gctgctggcg ctaacaaagt tgctgttatc aaagcagtac gtggcgcaac
     8821 tggcctgggt ctgaaagaag ctaaagactt ggtagaatct gcaccggctg ctctgaaaga
     8881 aggcgtgagc aaagacgacg cagaagcact gaaaaaagct ctggaagaag ctggcgctga
     8941 agttgaagtt aaataagcca acccttccgg ttgcagcctg agaaatcagg ctgatggctg
     9001 gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc cagtagcgtt tcacactgtt
     9061 tgactactgc tgtgcctttc aatgcttgtt tctatcgacg acttaatata ctgcgacagg
     9121 acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg tgatagcaac aggcattgcg
     9181 gaaagtgttc cattttccgg tcaacaaaat agtgttgcac aaactgtccg ctcaatggac
     9241 agatgggtcg acttgtcagc gagctgagga accctatggt ttactcctat accgagaaaa
     9301 aacgtattcg taaggatttt ggtaaacgtc cacaagttct ggatgtacct tatctccttt
     9361 ctatccagct tgactcgttt cagaaattta tcgagcaaga tcctgaaggg cagtatggtc
     9421 tggaagctgc tttccgttcc gtattcccga ttcagagcta cagcggtaat tccgagctgc
     9481 aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt ccaggaatgt caaatccgtg
     9541 gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct ggtgatctat gagcgcgaag
     9601 cgccggaagg caccgtaaaa gacattaaag aacaagaagt ctacatgggc gaaattccgc
     9661 tcatgacaga caacggtacc tttgttatca acggtactga gcgtgttatc gtttcccagc
     9721 tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg taaaacccac tcttcgggta
     9781 aagtgctgta taacgcgcgc atcatccctt accgtggttc ctggctggac ttcgaattcg
     9841 atccgaagga caacctgttc gtacgtatcg accgtcgccg taaactgcct gcgaccatca
     9901 ttctgcgcgc cctgaactac accacagagc agatcctcga cctgttcttt gaaaaagtta
     9961 tctttgaaat ccgtgataac aagctgcaga tggaactggt gccggaacgc ctgcgtggtg
    10021 aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta cgtagaaaaa ggccgccgta
    10081 tcactgcgcg ccacattcgc cagctggaaa aagacgacgt caaactgatc gaagtcccgg
    10141 ttgagtacat cgcaggtaaa gtggttgcta aagactatat tgatgagtct accggcgagc
    10201 tgatctgcgc agcgaacatg gagctgagcc tggatctgct ggctaagctg agccagtctg
    10261 gtcacaagcg tatcgaaacg ctgttcacca acgatctgga tcacggccca tatatctctg
    10321 aaaccttacg tgtcgaccca actaacgacc gtctgagcgc actggtagaa atctaccgca
    10381 tgatgcgccc tggcgagccg ccgactcgtg aagcagctga aagcctgttc gagaacctgt
    10441 tcttctccga agaccgttat gacttgtctg cggttggtcg tatgaagttc aaccgttctc
    10501 tgctgcgcga agaaatcgaa ggttccggta ttctgagcaa agacgacatc attgatgtta
    10561 tgaaaaagct catcgatatc cgtaacggta aaggcgaagt cgatgatatc gaccacctcg
    10621 gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa ccagttccgc gttggcctgg
    10681 tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg cgatctggat accctgatgc
    10741 ctcaggatat gatcaacgcc aagccgattt ccgcagcagt gaaagagttc ttcggttcca
    10801 gccagctgtc tcagtttatg gaccagaaca acccgctgtc tgagattacg cacaaacgtc
    10861 gtatctccgc actcggccca ggcggtctga cccgtgaacg tgcaggcttc gaagttcgag
    10921 acgtacaccc gactcactac ggtcgcgtat gtccaatcga aacccctgaa ggtccgaaca
    10981 tcggtctgat caactctctg tccgtgtacg cacagactaa cgaatacggc ttccttgaga
    11041 ctccgtatcg taaagtgacc gacggtgttg taactgacga aattcactac ctgtctgcta
    11101 tcgaagaagg caactacgtt atcgcccagg cgaactccaa cctggatgaa gaaggccact
    11161 tcgtagaaga cctggtaact tgccgtagca aaggcgaatc cagcttgttc agccgcgacc
    11221 aggttgacta catggacgta tccacccagc aggtggtatc cgtcggtgcg tccctgatcc
    11281 cgttcctgga acacgatgac gccaaccgtg cattgatggg tgcgaatatg caacgtcagg
    11341 ccgttccgac tctgcgcgct gataagccgc tggttggtac tggtatggaa cgtgctgttg
    11401 ccgttgactc cggtgtaact gcggtagcta aacgtggtgg tgtcgttcag tacgtggatg
    11461 cttcccgtat cgttatcaaa gttaacgaag acgagatgta tccgggtgaa gcaggtatcg
    11521 acatctacaa cctgaccaaa tacacccgtt ctaaccagaa cacctgtatc aaccagatgc
    11581 cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt gctggcagac ggtccgtcca
    11641 ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt agcgttcatg ccgtggaatg
    11701 gttacaactt cgaagactcc atcctcgtat ccgagcgtgt tgttcaggaa gaccgtttca
    11761 ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga caccaagctg ggtccggaag
    11821 agatcaccgc tgacatcccg aacgtgggtg aagctgcgct ctccaaactg gatgaatccg
    11881 gtatcgttta cattggtgcg gaagtgaccg gtggcgacat tctggttggt aaggtaacgc
    11941 cgaaaggtga aactcagctg accccagaag aaaaactgct gcgtgcgatc ttcggtgaga
    12001 aagcctctga cgttaaagac tcttctctgc gcgtaccaaa cggtgtatcc ggtacggtta
    12061 tcgacgttca ggtctttact cgcgatggcg tagaaaaaga caaacgtgcg ctggaaatcg
    12121 aagaaatgca gctcaaacag gcgaagaaag acctgtctga agaactgcag atcctcgaag
    12181 cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg tggcgttgaa gctgagaagc
    12241 tcgacaaact gccgcgcgat cgctggctgg agctgggcct gacagacgaa gagaaacaaa
    12301 atcagctgga acagctggct gagcagtatg acgaactgaa acacgagttc gagaagaaac
    12361 tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct ggcaccgggc gtgctgaaga
    12421 ttgttaaggt atatctggcg gttaaacgcc gtatccagcc tggtgacaag atggcaggtc
    12481 gtcacggtaa caagggtgta atttctaaga tcaacccgat cgaagatatg ccttacgatg
    12541 aaaacggtac gccggtagac atcgtactga acccgctggg cgtaccgtct cgtatgaaca
    12601 tcggtcagat cctcgaaacc cacctgggta tggctgcgaa aggtatcggc gacaagatca
    12661 acgccatgct gaaacagcag caagaagtcg cgaaactgcg cgaattcatc cagcgtgcgt
    12721 acgatctggg cgctgacgtt cgtcagaaag ttgacctgag taccttcagc gatgaagaag
    12781 ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat cgcaacgccg gtgttcgacg
    12841 gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg cgacctgccg acttccggtc
    12901 agatccgcct gtacgatggt cgcactggtg aacagttcga gcgtccggta accgttggtt
    12961 acatgtacat gctgaaactg aaccacctgg tcgacgacaa gatgcacgcg cgttccaccg
    13021 gttcttacag cctggttact cagcagccgc tgggtggtaa ggcacagttc ggtggtcagc
    13081 gtttcgggga gatggaagtg tgggcgctgg aagcatacgg cgcagcatac accctgcagg
    13141 aaatgctcac tgttaagtct gatgacgtga acggtcgtac caagatgtat aaaaacatcg
    13201 tggacggcaa ccatcagatg gagccgggca tgccagaatc cttcaacgta ttgttgaaag
    13261 agattcgttc gctgggtatc aacatcgaac tggaagacga gtaattctcg ctcaaacagg
    13321 tcactgctgt cggggtaaaa cccggcagcg gattgtgcta actccgacgg gagcaaatcc
    13381 gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc
    13441 aaaattgctc tggcttcgcc agacatgatc cgttcatggt ctttcggtga agttaaaaag
    13501 ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt
    13561 atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac
    13621 cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag
    13681 cgtatgggcc acatcgaact ggcttccccg actgcgcaca tctggttcct gaaatcgctg
    13741 ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac
    13801 tttgaatcct atgtggttat cgaaggcggt atgaccaacc tggaacgtca gcagatcctg
    13861 actgaagagc agtatctgga cgcgctggaa gagttcggtg acgaattcga cgcgaagatg
    13921 ggtgcggaag caatccaggc tctgctgaag agcatggatc tggagcaaga gtgcgaacag
    13981 ctgcgtgaag agctgaacga aaccaactcc gaaaccaagc gtaaaaagct gaccaagcgt
    14041 atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac cagagtggat gatcctgacc
    14101 gttctgccgg tactgccgcc agatctgcgt ccgctggttc cgctggatgg tggtcgtttc
    14161 gcgacttctg acctgaacga tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa
    14221 cgtctgctgg atctggctgc gccggacatc atcgtacgta acgaaaaacg tatgctgcag
    14281 gaagcggtag acgccctgct ggataacggt cgtcgcggtc gtgcgatcac cggttctaac
    14341 aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta aacagggtcg tttccgtcag
    14401 aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg taatcaccgt aggtccatac
    14461 ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc tggagctgtt caaaccgttc
    14521 atctacggca agctggaact gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg
    14581 gttgagcgcg aagaagctgt cgtttgggat atcctggacg aagttatccg cgaacacccg
    14641 gtactgctga accgtgcacc gactctgcac cgtctgggta tccaggcatt tgaaccggta
    14701 ctgatcgaag gtaaagctat ccagctgcac ccgctggttt gtgcggcata taacgccgac
    14761 ttcgatggtg accagatggc tgttcacgta ccgctgacgc tggaagccca gctggaagcg
    14821 cgtgcgctga tgatgtctac caacaacatc ctgtccccgg cgaacggcga accaatcatc
    14881 gttccgtctc aggacgttgt actgggtctg tactacatga cccgtgactg tgttaacgcc
    14941 aaaggcgaag gcatggtgct gactggcccg aaagaagcag aacgtctgta tcgctctggt
    15001 ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac
    15061 ggtgaattag tagcgaaaac cagcctgaaa gacacgactg ttggccgtgc cattctgtgg
    15121 atgattgtac cgaaaggtct gccttactcc atcgtcaacc aggcgctggg taaaaaagca
    15181 atctccaaaa tgctgaacac ctgctaccgc attctcggtc tgaaaccgac cgttattttt
    15241 gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc gttctggtgc atctgttggc
    15301 atcgatgaca tggtcatccc ggagaagaaa cacgaaatca tctccgaggc agaagcagaa
    15361 gttgctgaaa ttcaggagca gttccagtct ggtctggtaa ctgcgggcga acgctacaac
    15421 aaagttatcg atatctgggc tgcggcgaac gatcgtgtat ccaaagcgat gatggataac
    15481 ctgcaaactg aaaccgttat taaccgtgac ggtcaggaag agaagcaggt ttccttcaac
    15541 agcatctaca tgatggccga ctccggtgcg cgtggttctg cggcacagat tcgtcagctt
    15601 gctggtatgc gtggtctgat ggcaaagccg gatggctcca tcatcgaaac gccaatcacc
    15661 gcgaacttcc gtgaaggtct gaacgtactc cagtacttca tctccaccca cggtgctcgt
    15721 aaaggtctgg cggataccgc actgaaaact gcgaactccg gttacctgac tcgtcgtctg
    15781 gttgacgtgg cgcaggacct ggtggttacc gaagacgatt gtggtaccca tgaaggtatc
    15841 atgatgactc cggttatcga ggggggtgac gttaaagagc cgctgcgcga tcgcgtactg
    15901 ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg ctgatattct cgttccgcgc
    15961 aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag agaactctgt cgacgcggta
    16021 aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg tatgtgcgca ctgctacggt
    16081 cgtgacctgg cgcgtggcca catcatcaac aaaggtgaag caatcggtgt tatcgcggca
    16141 cagtccatcg gtgaaccggg tacacagctg accatgcgta cgttccacat cggtggtgcg
    16201 gcatctcgtg cggctgctga atccagcatc caggtgaaaa acaaaggtag catcaagctc
    16261 agcaacgtga agtcggttgt gaactccagc ggtaaactgg ttatcacttc ccgtaacacc
    16321 gaactgaaac tgatcgacga attcggtcgt accaaagaaa gctacaaagt gccttacggt
    16381 gcggtactgg cgaaaggcga tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg
    16441 gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg
    16501 atcgacggcc agaccattac tcgtcagacc gacgaactga ccggtctgtc ttcgctggtg
    16561 gttctggatt ccgcagaacg taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc
    16621 gttgatgctc agggtaacga cgttctgatc ccaggtaccg atatgcctgc gcagtacttc
    16681 ctgccgggta aagcgattgt tcagctggaa gatggcgtac agatcagctc tggtgacacc
    16741 ttggcgcgta ttccgcagga atccggcggt accaaggaca tcaccggtgg tctgccgcgc
    16801 gttgcggacc tgttcgaagc tcgtcgtccg aaagagccgg caatcctggc tgaaatcagc
    16861 ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc gtctggttat caccccggta
    16921 gacggtagcg atccgtacga agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa
    16981 ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt
    17041 ctgcgtctgc gtggtgttca tgctgtgact cgttacatcg ttaacgaagt acaggacgta
    17101 taccgtctgc agggcgtaaa gattaacgat aaacacatcg aagttatcgt tcgtcagatg
    17161 ctgcgtaaag ctaccatcgt taacgcgggc agctccgact tcctggaagg cgaacaggtt
    17221 gaatactctc gcgtcaagat cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca
    17281 acttactccc gcgatctgct gggtatcacc aaagcgtctc tggcaaccga gtccttcatc
    17341 tccgcggcat cgttccagga gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa
    17401 cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt
    17461 accggttacg cgtaccacca ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct
    17521 gcaccgcagg tgactgcaga agacgcatct gccagcctgg cagaactgct gaacgcaggt
    17581 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg
    17641 cgggtttttt tatgggtgtt attcagaggg caaattcttc acggaaaata ttaagaaagg
    17701 cagggcgaaa cgaggaagaa gcttttcgcc ctggacaaca ttcctgctga cggcactacc
    17761 ataaaaaaac acaacgcttt caacaacctt gcgtttttca tcttccgtag cggctcggaa
    17821 taaatttacg ttcttacatt ccgtctcata gtctttgcga ggcgcgtccc agatagctgt
    17881 cccagtcttt ccataccggc tgcaaaccct gagcggttaa cacggcagca accgcttccg
    17941 gtctgcgatc gtcgtgcggt gagaactgtt ccaactcggg gtgattatcg gcatagccac
    18001 ctggctgcgt tttcgagaag gcgctgacgt tattgatcgc cagcggaata acgcgatcgc
    18061 gaaaccacgg tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagtcgga
    18121 aggcgcagat ggtttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc
    18181 cagtacacgg gcgcaggcgc ggaaaggaga cagagtaacg gctttgccag taatgctgtt
    18241 gcagccatag caaatgttcg gcaaccatat agcagtcaac gcgccagttg tcggaaaggc
    18301 caattagcgc gccgaggcct atcttatcaa tccccgcacg ccccagccga tccggcgttt
    18361 ccagccgcca gaagaagtcc tgttttttgc ctttcaaatg atggcgggta taagtcgcct
    18421 cgtgatatgt ctcctgataa accatcaccc catccagacc aagttgcttt aactcggcgt
    18481 attccgtctc cgccagcggt tgcacttcca tttgtagtga agagaactgt tcacgcaggg
    18541 cggggagatg acgacgaaag taatccatcc ccactttcgc ctgatgttca ccagtgacta
    18601 atagcagatg ttcaaagccc atctcccgta tagcggcgct ttccctggca atatccgctt
    18661 catccagcgt tttgcgcttg atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt
    18721 tagcgcaaag attggaaaga taaagcggga cgtagaaact aactgtgttg ccaaatcgct
    18781 gacgggtcag acgctgcgcc tgttgggcca gttgttccag atagccactg gcggcaggcg
    18841 ataacagcgc catcatgtcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt
    18901 cagcagccgt tttgccgttg atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc
    18961 gattgctgaa ggttttcatg ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg
    19021 agcaaaataa ctgcggctgc ccggtccgga ctgacgtgcc agcaggccta cttctaccgc
    19081 cagacgaaat gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac
    19141 taacaccgcg tcggccccca tttccagcgc ctgcgtggca tggctgggaa cgccgatgcc
    19201 agcatcgaca accaccggaa ctgtggcctg ctggataata atctccagca tggcacgggt
    19261 ttccagtccc tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac
    19321 ttcttccaga cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc
    19381 ctgttgtacc agcgtttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg
    19441 ggcgtcaggg tgaatctcca attttagcca gtttgtgcct aacgcttcac gtgccagatg
    19501 ggcggcgaaa atggcttctt ccgctgtttt cgccccggaa gtattcggca gcagggccac
    19561 acccgccgcg ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt
    19621 catcgccagt gtcaccagct ggctgccgga agcgcggatc gcctccacca tcagttgtga
    19681 agaagcgaat ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg
    19741 taacatctca accccctgca ataacctgaa aaagcaggat ctggtcgcca tcctgcacga
    19801 tatgttgcgt ccactgctca cgcgggacga tttgctgatt aatcgccaga gccgcgcccg
    19861 cttgtcgttg gtcgagttgc tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca
    19921 tcggttgatc gttaaacagg atctgcattg cttcctccgc ataccgggca accactggcg
    19981 cggcgcaacg ccaggctgcg ccactggctc gacttaccgt cgaacagtcg gagttctccc
    20041 gcaggcgtct ctataccgct taataactta atggcttcca gtgcctgcaa agtgcccatc
    20101 accccgacca ccgggccaac cacgcccgcc gtgcggcagt tgcgttctgg ctgctggtta
    20161 tctggccaca ggcagcggta acacccctgc tcccagggcg gtgtcagtac catcaactga
    20221 ccgccaaatc cgaccgcgct ggcggtgata agcggcgtgt tgagtgccac gcaggcggca
    20281 ttaatctcct ggcgagtcgc catattgtcg gtacagtcga gcaccacatc ggcttgtgca
    20341 accgcatctt tcaacgcctc acccgttaac cgttgttgta atgctgtcag ttgaatgtcg
    20401 ggattcaact gtgtcagtcg ctgttggctg acctgcgatt tcgggctgtt gatatcttca
    20461 gtggtaaaga ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt
    20521 accagcgtcc cgacgccagc ccctgccagg tatagcgcag caggtgtacc cagcccgcca
    20581 agaccaataa tcagcacctg gctgtcgagc agtttttgct gcccgtcaag agcgatatcg
    20641 tcgagcagaa tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc
    20701 aatttccagc aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac
    20761 gacggcgata ctgccgacac ccgttgctat caccgcaggc gcgcgtgcca gactgatacc
    20821 gccaatcgcc acggtgggat aatccgccag gcgctcaaca tgccgtgcca actgttccag
    20881 cccctgcggt gcagaaggca tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat
    20941 ataagagggg cgtgctgcca gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc
    21001 cagccgcagg cctgccgcgc ggatggcatt gagatcggtg gcttgcaaat cttcctgccc
    21061 caaatggacg ccatacgcct gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa
    21121 tcgcgcgtta tagcggcggc ccagcgcaat tgccgccacg acatcggctt ccacctcttc
    21181 atcgcgccga tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc
    21241 gatccactgt acgctgtcca ccaccgggta cagtcctaaa cgaaaaggta caggaggaaa
    21301 atcaggctga tacatcacgc ttcctcctta tgcaggtaga tttctccgcc tctggcacgg
    21361 aagttctccg acatatccgc cattcccact tcaatggttt gcgcggcggc gtaatcgcgt
    21421 acttcctggc tgattttcat cgagcagaat tttggcccac acatggagca aaaatgggcg
    21481 actttgccgg actcttgggg cagggtttca tcgtgataag cgcgggcggt aaacgggtcg
    21541 agggccagat taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta
    21601 tcgcgaattt gcgcgcccgg atgccctttt gccaggtcag cagcgtgggc ggcaatctta
    21661 taagtgataa gcccctgctt aacatcttct ttattgggca gacccagatg ctcttttggc
    21721 gttacgtaac agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccccgac
    21781 gtgaagtggt catagcccgg tgcaatatcg gtagttagcg gccccagagt gtaaaacggc
    21841 gcttcgtggc agtgctctaa ctcctcggtc atattgcggc ggatcatctg catcggcaca
    21901 tggcctgggc cttcaatcat cacctgtacg tcatattccc aggcgatttt ggtcagttcg
    21961 cccagcgtat gcaattcggc aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg
    22021 cgcagaccgt cgcccagcga cagcgaaacg tcataagcgg cacagatttc acaaatttcg
    22081 cggaagtgtt gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc
    22141 gaaccgccgc gagagacgat accggtcagg cgtttcgcgg tcatcggcac atagcgcagc
    22201 agtacgcccg catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagcgtg
    22261 tcgcggaacg cttcccaggt aagatcttcg gcgatcccgt taaccttctc cagcgcctgg
    22321 tagatcggca ctgtaccgat cggcaccgga ctgttacgca aaatccactc gcgggtttcg
    22381 tgaatatagc gaccggtgga gagatccatc accgtatccg ctccccagcg cgtggaccat
    22441 accagctttt ccacttcttc ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg
    22501 ttaactttta ccaggaagtt acggccaata atcatcggct ccgattccgg atgattaatg
    22561 ttggccggga taatcgcacg tccggcagca acttcatcac ggacaaattc cgcagtgata
    22621 ttttccggca gatgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacctcgcta
    22681 cggatgcgct cgcggcccat attctcgcgg atggcgatga attccatgtc cggcgtgatg
    22741 atgccctggc gggcgtagtg cagttgggtg acacggcgtc ctgttttggc gcgttttggc
    22801 gttaataagc cgctaaaacg tagctcgtcg agaccatcat ctgccagccg cgttctggtg
    22861 taatcggaac tgcgcacggt aagttcttcg gtatcggcgc gcgcatcgat ccacggctgg
    22921 cgtagttttg ccagcccttg ctgcacgtta atggcgatct gcggatcacc atacgggccg
    22981 gaggtgtcgt agaccggaat cgcttcgttt tcttcgtact gcggctgttc tttgctaccg
    23041 ccaattagcg tcgggctaag ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg
    23101 ccagtgatat aaatgcgttt tgagttggga aaggcggtgc cttccagggt gtcgataaaa
    23161 tgttgggccc gggcgcgttg ttcgcggcgg gtcagttttg ttgcagacat agctcattcc
    23221 aaaaagttaa ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag
    23281 gcgatgcgat agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg
    23341 cggatcccga ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagcccccac
    23401 aaacggggcg ttgaatgtaa attacgcgtt aacagcgcag aactcaagca ggatgtttga
    23461 cgcgggcggc gtcaagcacc agcagaatca gcttatcttc cagcacaaag cgcgcttcca
    23521 gcgcctcgcc gatgtcagat aaaacctgtt gaaactcaag gtaattatca tgatcgatgg
    23581 cggtttccag gctggaatcg tagtaatcca taatctgttg ggtattggct tcgagttgcg
    23641 gccaaatctt cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga agaatgcgct
    23701 cataaatact gaaatgtccg gcagacaagt aatcgaccag gctctgacaa aaatcatcaa
    23761 gggctttttc gtttagcctc atgtacgatt ctttgccagg cttaatgcca accagattgt
    23821 agtaagccac gagcagatgc ttacgtacat gtagccagcg atcaaccagt ttgttacttc
    23881 ctctgacgcg ctccgtcagg ttatcgagct ggttaagcat gattgactcc gcaagtttgt
    23941 attcaaaaac tgctcagtga gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag
    24001 gtagtgcaag agctatggat cgtataattg aaaaattaga tcacggctgg tgggtcgtca
    24061 gccatgaaca aaaattatgg ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt
    24121 tcgatcttgt gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa cctgtttggt
    24181 taatacaaca gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg
    24241 ttgggctgtt tcaactggcc ggacgtggcg tgcaactggc ggagttttac cgatcgcata
    24301 aatactgtgg ttactgcggg catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt
    24361 gtagccattg ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt gttgccatcc
    24421 gtcgcgatga ttcgctcctc ctcgcccagc atacccgcca tcgtaacggt gtccatacag
    24481 tacttgccgg attcgtcgaa gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga
    24541 tggaagagag cggaattaaa gttaaaaact tgcgttacgt gacttctcag ccgtggccgt
    24601 ttcctcagtc tttaatgacc gcgtttatgg cggaatatga cagcggcgac atcgtgatcg
    24661 acccgaaaga attgctcgag gcgaactggt atcgctatga cgatttgccg ttactcccgc
    24721 cgcccggcac cgtagcgcgc cgtctgatag aagatactgt ggcgatgtgt cgggcagagt
    24781 atgagtgatg atacactgac cgcctgacgc actaaggaac agccaaaatg accgaactta
    24841 aaaacgatcg ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact ccagtatgga
    24901 tgatgcgcca ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc caggcgggcg
    24961 attttatgtc gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg caaccgctgc
    25021 gtcgctaccc gctggatgcg gcgatcctct tttccgatat cctcaccgtg ccggacgcga
    25081 tggggttagg gctctatttt gaagccggag aaggtccgcg ttttacctcg ccagtcacct
    25141 gcaaagccga cgtcgataaa ctgccaattc cggacccgga agatgagctg ggttacgtga
    25201 tgaacgcggt gcgtaccatt cgtcacgaac tgaaaggcga agtgccgctg attggttttt
    25261 ccggcagccc gtggacgctg gcaacctaca tggtggaagg cggcagcagc aaagccttca
    25321 ccgtgatcaa aaaaatgatg tatgccgatc cgcaggcgct gcacgctctg ctcgataaac
    25381 tggcgaaaag cgtcactttg tatctgaatg cgcagattaa agccggtgct caggcagtga
    25441 tgattttcga cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct
    25501 attacatgca taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag
    25561 tcacgctgtt taccaaaggc ggcggacagt ggctggaagc catggctgaa accggttgcg
    25621 atgcgctggg cctcgactgg acaacggaca tcgccgatgc gcgccgccgt gtgggcaata
    25681 aagtcgcgtt gcagggtaat atggatccgt cgatgctgta cgcgccgcct gcccgcattg
    25741 aagaagaagt agcgactata cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc
    25801 ttggtcacgg cattcatcag gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag
    25861 tgcatcgact gtctgaacag tatcaccgct aagagcctga tccatcaggc tattttattt
    25921 gccattttga ggccgggcag tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt
    25981 tccgcgctgt ccggcctcaa actgccttca acaattacgc ctgttgaacc aggctctaag
    26041 gagcgattat ggatctcgcg tcattacgcg ctcaacaaat cgaactggct tcttctgtga
    26101 tctgcgagga tcgactcgat aaagatccac cggatctgat cgccggagcc gatgtcgggt
    26161 ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg
    26221 agctggtgga gtataaagtt gcccgcatcg ccaccaccat gccttacatt ccaggttttc
    26281 tttccttccg cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg
    26341 atttagtgtt tgtcgatggt catgggatct cgcatcctcg ccgtcttggc gtcgccagcc
    26401 attttggctt attggtggat gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta
    26461 aattcgaacc gctctccagc aaaccgggcg cgctggcccc actgatggat aaaggcgagc
    26521 agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc
    26581 atcgggtcag cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc
    26641 tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct
    26701 atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa tttccgtatt tgagaactca
    26761 acatgttaca aaacccaatt catctgcgtc tggagcgcct ggaaagctgg cagcatgtca
    26821 ctttcatggc ttgcttatgc gaacgcatgt accccaatta cgccatgttc tgccagcaaa
    26881 ccggttttgg tgatgggcaa atttaccgtc gtattctcga tctcatctgg gaaacgctga
    26941 ccgttaaaga cgcaaaagta aatttcgaca gccaactgga gaaatttgaa gaagcgattc
    27001 cttcagccga cgatttcgat ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt
    27061 taagtgaact ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga
    27121 gtaagacctc catcacgacc gttgcgatgc tggaaatgac tcaggctggt cgcgaaatgg
    27181 gcgatgaaga gcttaaagaa aacccagctg tagagcaaga atgggacatt cagtgggaaa
    27241 tattccgact tttagctgag tgcgaagaac gcgatatcga gctgataaaa ggccttaggg
    27301 cagacctgcg tgagtcgggt gagagcaata ttggtataat ttttcagcaa taagaccaga
    27361 aaacgtgatt taacgcctga tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg
    27421 tctacatttg gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga gagtgctatt
    27481 ctggcatttc cgtcgcactc gatgcttagc aagcgataaa cacattgtaa ggataactta
    27541 tgaacaagac tcaactgatt gatgtaattg cagagaaagc agaactgtcc aaaacccagg
    27601 ctaaagctgc tctggagtcc actctggctg caattactga gtctctgaaa gaaggcgatg
    27661 ctgtacaact ggttggtttc ggtaccttca aagtgaacca tcgcgctgag cgtactggcc
    27721 gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt
    27781 ctggcaaggc actgaaagac gcagttaagt aagattgcgt ggcagtgaac agttttaacg
    27841 aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc attgatgctg gcaggtgcgc
    27901 tgtttctcac tgcctgtagc cacaactctt cacttcctcc ctttaccgcc agtggatttg
    27961 ctgaagacca gggcgcggta cgcatctggc gaaaagacag cggcgataat gtgcatctgc
    28021 ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac gcgagagtat cgctggcagg
    28081 gcgataacct cacgctcatc aatatcaatg tttacagcaa accgccggtg aacattcgcg
    28141 cgcgttttga cgatcgcggc gatctgagct ttatgcaacg tgaatccgat ggggaaaagc
    28201 agcagctttc taacgaccaa atcgatttat accgttatcg tgctgatcag atccgccaga
    28261 ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg
    28321 aacagaccgt gaccacctgc gaagggcaaa ccattaaacc tgatttagat tcgcaggcga
    28381 tagcgcatat cgagcgccgc cagagccgct cttctgttga tgtcagcgtg gcatggctgg
    28441 aagcgcccga aggttcgcaa ttactgttag tggcaaactc tgatttctgt cgctggcaac
    28501 ccaacgagaa aacgttctga tttaccagtg gcccataccc atatgaccgc caccaccgca
    28561 gccgccgtag cccatgccca ttccggctcc gcgcggaata cccgcttcag ccatcgcgat
    28621 atctcgtttc acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac
    28681 cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg
    28741 cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt
    28801 ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg
    28861 tccgccgtga gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac
    28921 cagggcaatt ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt
    28981 attgcatctt ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac
    29041 gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc
    29101 ctttgtgtta cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa
    29161 agggatgagc aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct
    29221 aaatggttaa gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg
    29281 acggtgattc gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa
    29341 ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg
    29401 catcatgcgc agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg
    29461 tttgcggtca cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga
    29521 aaacagcttt attcccctca ggaaatgcag cagttacatc cgggagatga agaagcgtgg
    29581 cggcggatcg atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg
    29641 atgtttgctg ctggaatgta ccggatgcgc catatgcagc agtatgccgc gacaccacaa
    29701 gcaattttca tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga
    29761 aacaccctga ttattctctt cgccctggcg acggtcttgc tggcaagcgt attgtcattc
    29821 ttctggtatc gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa
    29881 gagaagctgg tggcgctggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca
    29941 ctttcctcga ttaaaggact ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa
    30001 gcgcatcaac tggcgcaggt gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc
    30061 gagttgctgg aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg
    30121 ctgattaacc actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta
    30181 cgctttaccg ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag
    30241 gtcctgttga atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc
    30301 gtgacggcca gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga
    30361 attgcggcag atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc
    30421 accggattgg ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag
    30481 gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg
    30541 cgtaaggacc cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca
    30601 ttagccactg cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg
    30661 cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt
    30721 gcgatgtgcg aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa
    30781 acccggcaat tccggtgctg attatgactg cgtactccag cgtcgagacg gcggtagagg
    30841 cactgaaaac tggggcgcag gattatctca tcaagccgct ggatttcgat aacctacagg
    30901 cgacgctgga aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga
    30961 ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa
    31021 tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggcaccggta
    31081 aagagctggt cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa
    31141 cgctcaactg tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa
    31201 aaggggcgtt tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg
    31261 gcacgctgtt tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc
    31321 gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg
    31381 tccggctgat tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc
    31441 gccaggatct ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac
    31501 ggcgggaaga tatccctctg ctggctggcc attttcttca gcgctttgcc gagcgtaatc
    31561 gcaaggcggt aaaaggcttt acgccacagg cgatggattt gctgatccac tacgactggc
    31621 cgggaaatat tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg
    31681 aatatatttc cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggggc
    31741 aaagtcagga tattcagccg ctggtggaag tggaaaaaga ggtgattctg gcggcgctgg
    31801 agaaaacggg cggcaacaaa accgaagccg cccgtcagtt agggatcacg cgcaaaacgc
    31861 tattggcaaa actgtcgcgt tagttctgct cgcgttcgat ggcgcgccaa ccgatatctt
    31921 tccggcagaa gcaatcatcc cagtggatat cggtcattaa ggcgtaggcg cgtttctgcg
    31981 cttctgccac ggtatgaccc agcgcggtga cgcacagtac gcgcccgccg ctggttacca
    32041 cctgctcgtc atccgccagt tttgtgcccg cgtggaacac tttgccgtca gccacttctt
    32101 ccagcggcag gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag
    32161 ccatcaccac gctgagagaa gcacgttcat cccactctga cgttttctcg tccagtttgc
    32221 cttcacaggc cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct
    32281 gggtttccgg atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt
    32341 cgatcatcag gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt
    32401 tcacggttgg ccagatgata cgttccatgg tgcgctgatg aacttcatcg gttactaccg
    32461 gcgcggggga gtaagcgccc atcccgccgg tgtttggtcc gttatcttta tcgcctacgc
    32521 gtttgtgatc ctggctggta gccatcggca gcacatgctc gccgtccacc atcacgataa
    32581 agctcgcttc ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa
    32641 aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca
    32701 cgataacgcc tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct
    32761 cacgcagata caccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga
    32821 tcttatggcg ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac
    32881 ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca
    32941 ccagcggcgc ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca
    33001 acagcgccgg gatatcggtc acgccgatag caacgttttg cagcgcgggt tccagcgcag
    33061 tgcctgcatt acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc
    33121 aggccagcgc gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca
    33181 ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc
    33241 ggcaatcact tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc
    33301 agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga
    33361 acctttcact tccaggcctt catcggcagc tttaataccg gcgattttcg cggagtacac
    33421 gcggctcatc tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc
    33481 attggatttc acgaatttcg ccactttcca gcagaacagc gcatcacgca gttcctgttc
    33541 ggtcggctga cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg
    33601 atcctgaacc agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc
    33661 ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc
    33721 ttcgctggcg gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc
    33781 ggtttccgca tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc
    33841 ggttttgtac gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg
    33901 gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc
    33961 cgcatcggta tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt
    34021 agcaacggag gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt
    34081 ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg
    34141 accggcggct tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc
    34201 aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt
    34261 tgcaagcgtc agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct
    34321 gctcttcacc acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg
    34381 gccgccgata tcgatgttct caaccgcatc ttccagcgag cagccttcac gagcaacagt
    34441 ctgggcgaac ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc
    34501 cataatggca tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag
    34561 ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac
    34621 cggcagacct ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc
    34681 gcgtgcggaa agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag
    34741 agcgcggcgg actggacgac gttgttgcat ggtaaatccc ctgggtttga ctattacaga
    34801 gagcgttagc tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat
    34861 ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat
    34921 ggtgatcaca attttgacag tggttaccgt gggaaaaata cagaaattac attgatgatt
    34981 gtggataact ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc
    35041 agtcattttt ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgccttc
    35101 atcgacacgg cggatgtgaa tcacttcaca caaacagccg gttcggttga agaga
//
LOCUS       DAECNO010000034        35012 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.034, whole genome shotgun sequence.
ACCESSION   DAECNO010000034 DAECNO010000000
VERSION     DAECNO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.034"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..828)
                     /locus_tag="KKO08_001713"
     CDS             complement(<1..828)
                     /locus_tag="KKO08_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD6317992.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     "
     gene            920..1006
                     /locus_tag="KKO08_001714"
                     /pseudo
     CDS             920..1006
                     /locus_tag="KKO08_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1411..1887)
                     /locus_tag="KKO08_001715"
     CDS             complement(1411..1887)
                     /locus_tag="KKO08_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBD6317993.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1946..3235)
                     /gene="bioA"
                     /locus_tag="KKO08_001716"
     CDS             complement(1946..3235)
                     /gene="bioA"
                     /locus_tag="KKO08_001716"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBD6317994.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3322..4362
                     /gene="bioB"
                     /locus_tag="KKO08_001717"
     CDS             3322..4362
                     /gene="bioB"
                     /locus_tag="KKO08_001717"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBD6317995.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4359..5513
                     /gene="bioF"
                     /locus_tag="KKO08_001718"
     CDS             4359..5513
                     /gene="bioF"
                     /locus_tag="KKO08_001718"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBD6317996.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5500..6255
                     /gene="bioC"
                     /locus_tag="KKO08_001719"
     CDS             5500..6255
                     /gene="bioC"
                     /locus_tag="KKO08_001719"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBD6317997.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6248..6925
                     /gene="bioD"
                     /locus_tag="KKO08_001720"
     CDS             6248..6925
                     /gene="bioD"
                     /locus_tag="KKO08_001720"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBD6317998.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7504..9525
                     /gene="uvrB"
                     /locus_tag="KKO08_001721"
     CDS             7504..9525
                     /gene="uvrB"
                     /locus_tag="KKO08_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBD6317999.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9717..10625)
                     /gene="yvcK"
                     /locus_tag="KKO08_001722"
     CDS             complement(9717..10625)
                     /gene="yvcK"
                     /locus_tag="KKO08_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBD6318000.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10890..11034
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11022..12011
                     /gene="moaA"
                     /locus_tag="KKO08_001723"
     CDS             11022..12011
                     /gene="moaA"
                     /locus_tag="KKO08_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBD6318001.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12033..12545
                     /gene="moaB"
                     /locus_tag="KKO08_001724"
     CDS             12033..12545
                     /gene="moaB"
                     /locus_tag="KKO08_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBD6318002.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12548..13033
                     /gene="moaC"
                     /locus_tag="KKO08_001725"
     CDS             12548..13033
                     /gene="moaC"
                     /locus_tag="KKO08_001725"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBD6318003.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13026..13271
                     /gene="moaD"
                     /locus_tag="KKO08_001726"
     CDS             13026..13271
                     /gene="moaD"
                     /locus_tag="KKO08_001726"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBD6318004.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13273..13725
                     /gene="moaE"
                     /locus_tag="KKO08_001727"
     CDS             13273..13725
                     /gene="moaE"
                     /locus_tag="KKO08_001727"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBD6318005.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13862..14566
                     /locus_tag="KKO08_001728"
     CDS             13862..14566
                     /locus_tag="KKO08_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD6318006.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14771..15484
                     /locus_tag="KKO08_001729"
     CDS             14771..15484
                     /locus_tag="KKO08_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBD6318007.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15520..16476)
                     /locus_tag="KKO08_001730"
     CDS             complement(15520..16476)
                     /locus_tag="KKO08_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBD6318008.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16476..17717)
                     /gene="clsB"
                     /locus_tag="KKO08_001731"
     CDS             complement(16476..17717)
                     /gene="clsB"
                     /locus_tag="KKO08_001731"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBD6318009.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17714..18475)
                     /locus_tag="KKO08_001732"
     CDS             complement(17714..18475)
                     /locus_tag="KKO08_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD6318010.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18608..19018
                     /locus_tag="KKO08_001733"
     CDS             18608..19018
                     /locus_tag="KKO08_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBD6318011.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18980..20086)
                     /locus_tag="KKO08_001734"
     CDS             complement(18980..20086)
                     /locus_tag="KKO08_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6318012.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20097..21230)
                     /locus_tag="KKO08_001735"
     CDS             complement(20097..21230)
                     /locus_tag="KKO08_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6318013.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21223..22959)
                     /locus_tag="KKO08_001736"
     CDS             complement(21223..22959)
                     /locus_tag="KKO08_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6318014.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22952..23947)
                     /gene="hlyD"
                     /locus_tag="KKO08_001737"
     CDS             complement(22952..23947)
                     /gene="hlyD"
                     /locus_tag="KKO08_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBD6318015.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23950..24621)
                     /gene="cecR"
                     /locus_tag="KKO08_001738"
     CDS             complement(23950..24621)
                     /gene="cecR"
                     /locus_tag="KKO08_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBD6318016.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24850..26037
                     /gene="rhlE"
                     /locus_tag="KKO08_001739"
                     /pseudo
     CDS             24850..26037
                     /gene="rhlE"
                     /locus_tag="KKO08_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26244..26726)
                     /gene="ybiA"
                     /locus_tag="KKO08_001740"
     CDS             complement(26244..26726)
                     /gene="ybiA"
                     /locus_tag="KKO08_001740"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBD6318017.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26846..28996
                     /gene="dinG"
                     /locus_tag="KKO08_001741"
     CDS             26846..28996
                     /gene="dinG"
                     /locus_tag="KKO08_001741"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBD6318018.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29024..29986
                     /gene="ybiB"
                     /locus_tag="KKO08_001742"
     CDS             29024..29986
                     /gene="ybiB"
                     /locus_tag="KKO08_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBD6318019.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30127..31212
                     /gene="hcxB"
                     /locus_tag="KKO08_001743"
     CDS             30127..31212
                     /gene="hcxB"
                     /locus_tag="KKO08_001743"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBD6318020.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31441..31701)
                     /locus_tag="KKO08_001744"
     CDS             complement(31441..31701)
                     /locus_tag="KKO08_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6318021.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31966..32232)
                     /gene="ybiI"
                     /locus_tag="KKO08_001745"
     CDS             complement(31966..32232)
                     /gene="ybiI"
                     /locus_tag="KKO08_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBD6318022.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32306..32983)
                     /gene="ybiX"
                     /locus_tag="KKO08_001746"
     CDS             complement(32306..32983)
                     /gene="ybiX"
                     /locus_tag="KKO08_001746"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBD6318023.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33025..35012)
                     /locus_tag="KKO08_001747"
                     /pseudo
     CDS             complement(33025..35012)
                     /locus_tag="KKO08_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8297 a         8977 c         9463 g         8275 t
ORIGIN      
        1 gtggtagtcc ggcgaagagg ttaatctttg cagggcttgc agagtgcgag tcgacaatgg
       61 attgttataa atattaacat ctgcataaat gtgcagattc atgatactgt ctggcaatgt
      121 gttcagctga ttgttaggga tatatagtga ttgcagacgt tgtggaagtt cgggcagtct
      181 gtgtagttgg ttttccccaa cgtttaaaag cctcagattt tgaggaaggg gcggtagctc
      241 ttgtaatctg ttatcaaaag cacttagttc ctgtagttca catggtaatt ctggtaaggc
      301 agtaagctgg ttgttgccaa cagaaagttt ttgtagagcc gggggaagat caggtagcgt
      361 ctccaggcta ttgagaaggg ctgagagtga ctgcaaggaa gatggaagag aaggcaaaca
      421 tgttaaaact ctgttaagtg aaacatcaag agcgaccagg tgaggcggaa gagccgggag
      481 cctgctcagt ctgttatcgc tggctttaag tacagttaag gacggaggga gttctggcag
      541 agagcgtagc tcattgctag agatgttaag ttcttgtatg tgcgggggca ggtatgggag
      601 agagcgtaat ctgagtaaac ttaaattgag ggctggctct tgaaaagcca gacatatttt
      661 cagtaatcga actgcctgtg ttcggtcttc tgttgcagca ccttccttgg cccagttatc
      721 ccagatgcgg tgataatccg tgatattttg ctcttctacg gataaacggg atatacatcc
      781 taaaccacaa aaggagcgga aaaaagaagg attgtgggca ggtttcattt attattgatc
      841 ctcattgttt atatattaat atattcgtgc aaatggagtg aggattcttt aaaaaaacgc
      901 gcctgtaaat agttaagaac aggaagaact gttggggatg tggctggccg aaaatgaagg
      961 ggtgaagttc tggctcaatg tgctgactga actgaaaaaa cgcggttggt cgccctacct
     1021 tttcatcaag ccagtagtct gcccaccaca gcatcatttc tctgcgctta tcgagatact
     1081 gagcatggtt gtaaattcag ctaacccagt gcgttgacaa aaaattagcg caagaggaca
     1141 aaaaatcacc ttgcgctaat gctctgtatc aggtcactaa taccatctaa gtggttgatt
     1201 catagtgact ggatatgttg tgttttgcag tattatgcag tctatttttt aagctaaatg
     1261 tattataatg cattgatatt aatgattttt actgtttctc ggtcagcttt tttatactaa
     1321 cttgagcgaa acgggaaggt aaaaagacaa aaagttgttt ttaatacctt taagtgatac
     1381 cagatggcat tgcgccatct ggcagagtga ttaactaaac atcgcggtaa tcgatgcgct
     1441 tgccagagag tggaaatgaa cgttaaaccc gaccatcgcg ccgctggctc cttcatcgac
     1501 atcaatacgc tctacatcca gcgcgtgaac ggtaaaaatg tagcgatggg tttcgccttt
     1561 cggcggcgct gcgccatcgt atccggtttt accaaaatcg gtacgcgtct gcaaaacgcc
     1621 gtctggcatt gctaccagac cagagccaaa cccttgcggt aatacgcggg tatcagcggg
     1681 taaattaaca actacccagt gccaccagcc ggagccggtt ggcgcatccg ggtcgtagca
     1741 ggtgacaaca aaacttttcg ttcccgcagg aacatcatcc cacgccagat gcggtgaaat
     1801 attatcgcca tcgtaaccca tgccgttaaa gacatgacga tgcggcaact tatcgccatc
     1861 gcgcagatcg ttactgatga gtttcatgaa ccctcctttc ttgtttgcag aaagtgtagc
     1921 cagaaaccct cacgctgact tcccgttatt ggcaaaaaaa tgtttcatcc tgtaccgcgc
     1981 ggttaaccgc tgcggtcaga cgctgcaact gttgcgggag aataatatag ggcggcatca
     2041 ggtaaatcag tttgccaaaa ggccggatcc ataccccctg ttcgacaaag aatttttgca
     2101 gcgccgccat attcaccgga cgagtggttt cgaccacacc gattgccccc agtacgcgca
     2161 catcggcaac catttcggca tcacgggctg gtgccagttg ctcgcgcagc tgcacttcaa
     2221 tagccgccac ctgctgctgc cattcgccgg attcgataat cgctaggctg gcgtttgctg
     2281 ccgcgcaggc cagcggattg cccataaaag ttggcccatg cataaagcag ccggcttcgc
     2341 cgttactgat ggtttctgca acctcgcgcg tggtaagtgt ggcggaaagg gtcattgtgc
     2401 cgccggttaa ggctttaccg aggcacaaaa tgtccggcgc gatttctgca tattcacagg
     2461 caaacagttt gccggtacga ccaaatccgg tggcgatctc gtcggcaatc agcaagatac
     2521 cttcgcgatc gcacattttg cggattcgtt ttaaccattc cggatggtac atgcgcatcc
     2581 cgcctgcgcc ctggacaatc ggctcaatga tcaccgctgc gatttcatga cgatgcgccg
     2641 ccatcaggcg ggcaaaacca accatatcgc gttcatccca ttcaccatcc atgcggcttt
     2701 gcggggcggg agcaaacaag ttttctggca gatagccttt ccacaggctg tgcattgagt
     2761 tgtccggatc gcacaccgac atcgcgccaa aggtatcgcc atgataacca ttgcggaagg
     2821 tcagaaaacg ctggcgcgct tcgcctttgg cttgccagta ctgcaacgcc attttcatcg
     2881 ccacttccac cgctacggaa ccggagtccg cgagaaaaac gcactccagc ggttgcggcg
     2941 tcattgccac cagtttgcgg catagctcga tggcgggcgc atgggtgata ccgccaaaca
     3001 tcacatgcga catggcatca atttgcgtct tcatcgccgc attaagctgc gggtgattgt
     3061 agccgtggat cgccgcccac caggacgaca taccgtcaac caggcgtctg ccgtcagaca
     3121 aaattagctc gcaaccttcg gcgctcgcca ccggataaac cggcagaggg gaggtcatgg
     3181 atgtgtatgg gtgccagata tggcgttggt caaaggcaag atcgtccgtt gtcataatcg
     3241 acttgtaaac caaattgaaa agatttaggt ttacaagcct acaccgaatt aacaacaaaa
     3301 aacacgtttt ggagaagccc catggctcac cgcccacgct ggacattgtc gcaagtcaca
     3361 gaattatttg aaaaaccgtt gctggatctg ctgtttgaag cgcagcaggt gcatcgccag
     3421 catttcgaac ctcgtcaggt gcaggtcagc acgttgctgt cgattaagac cggagcttgt
     3481 ccggaagatt gcaaatactg cccgcaaagc tcgcgctaca aaaccgggct ggaagccgag
     3541 cggttgatgg aagttgaaca ggtgctggag tcggcgcgca aagcgaaagc ggcaggatcg
     3601 acgcgcttct gtatgggcgc ggcatggaag aatccccacg aacgcgatat gccgtacctg
     3661 gaacaaatgg tgcagggggt aaaagcgatg gggctggagg cgtgtatgac gctgggcacg
     3721 ttgagtgaat ctcaggcgca gcgcctcgcg aacgccgggc tggattacta caaccacaac
     3781 ctggacacct cgccggagtt ttacggcaat atcatcacca cacgcactta tcaggaacgc
     3841 ctcgatacgc tggaaaaagt gcgcgatgcc gggatcaaag tctgctcagg tggcattgtg
     3901 ggcttaggcg aaacggtaaa agatcgcgcc ggattattgc tgcaactggc aaacctgccg
     3961 acgccgccgg aaagcgtacc aatcaacatg ctggtgaagg tgaaaggcac gccgcttgcc
     4021 gataacgatg acgttgatgc ctttgatttt attcgcacca ttgccgtagc gcggatcatg
     4081 atgccgacct cttacgtgcg cctttctgcc ggacgcgagc agatgaacga acagactcag
     4141 gcgatgtgct ttatggcagg cgcaaactcg attttctacg gttgcaaact gctgaccacg
     4201 ccgaatccgg aagaagataa agacctgcaa ctgttccgca aactggggct aaatccgcag
     4261 caaactgccg tgctggcagg ggataacgaa caacagcaac gtcttggaca ggcgctgatg
     4321 accccggaca ccgacgaata ttacaacgcg gcagcattat gagctggcag gagaaaatca
     4381 acgcggcgct cgatgcgcgg cgtgctgccg atgccctgcg tcgccgttat ccggtggcgc
     4441 aaggagccgg acgctggctg gtggcggatg atcgccagta tctgaacttt tccagtaacg
     4501 attatttagg tttaagccat catccgcaaa ttatccgtgc ctggaagcag ggggcggagc
     4561 aatttggcat cggtagcggc ggctccggtc acgtcagcgg ttatagcgtg gtgcatcagg
     4621 cgctggaaga agaactggcc gagtggcttg gctattcgcg ggcgctgctg tttatctctg
     4681 gttttgccgc gaatcaggca gtcattgccg cgatgatggc gaaagaggac cgtattgttg
     4741 ccgaccggct tagccatgcc tcattgctgg aggctgccag tttaagcccg tcgcagcttc
     4801 gccgttttac tcataacgat gtcgctcatc tggcgcgact gcttgcttcc ccctgtccgg
     4861 ggcagcaact ggtggtgaca gaaggcgtgt tcagcatgga cggcgatagc gcgccactgg
     4921 cggaaatcca gcaggtaacg caacagcaca atggctggtt gatggtcgat gatgcccacg
     4981 gcacgggcgt tatcggggag caggggcgcg gcagctgctg gctgcaaaag gtaaaaccag
     5041 aattgctggt agtgactttt ggcaaaggat ttggcgtcag tggggctgcg gtgctttgct
     5101 caagtacggt ggcggattat ctgctgcaat tcgcccgcca ccttatctac agcaccagta
     5161 tgccgcccgc tcaggcgcag gcattacgtg cgtcgctggc ggtcattcgc cgtgatgagg
     5221 gggatgcacg gcgcgaaaaa ctggtgtcac tcattgcgcg ttttcgtgcc ggagtacagg
     5281 atttgccgtt tacgctggct gattcatgca gcgccatcca gccattgatc gtcggtgata
     5341 acagccgtgc gttacaactg gcagaaaaac tgcgccagca aggctgctgg gtcacggcga
     5401 ttcgcccgcc aaccgtaccc gctggtactg cgcgactgcg cttaacgcta accgctgcgc
     5461 atgaaatgca ggatatcgac cgtctgctgg aggtgctgca tggcaacggt taataaacaa
     5521 gccattgcag cggcatttgg tcgggcagcc gcacactatg agcaacatgc agatctacag
     5581 cgccagagtg ctgacgcctt actggcaatg cttccacagc gtaaatacac ccacgtactg
     5641 gacgcgggtt gtggacctgg ctggatgagc cgccactggc gggaacgtca cgcgcaggtg
     5701 acggcgttag atctctcgcc gccaatgctt gcgcaagccc gccataaaga tgccgcagat
     5761 cattatctgg caggagatat cgaatccctg ccgttagcga ctgcgacgtt cgatcttgca
     5821 tggagcaatc tcgcggtgca gtggtgcggt aatttatcca cggcactccg cgagctgtat
     5881 cgggtggtgc gccccggagg cgtggtcgcg tttaccacgc tggtgcaggg atcgttaccc
     5941 gaactgcatc aggcgcggca ggcggtggac gagcgtccgc atgctaatcg ctttttaccg
     6001 ccagatgaaa tcgaagagtc gctgaacggc gtgcgttatc aacatcatat tcagctcatc
     6061 acactatggt ttgatgatgc gctcagtgcc atgcgttcgc tgaaaggcat cggtgctacg
     6121 catcttcatg aagggcgcga cccgcgaata ttaacgcgtt cgcagttgca gcgattgcaa
     6181 ctggcctggc cgcaacaaca ggggcgatat cctctgacgt atcatctttt tttgggagtg
     6241 attgctcgtg agtaaacgtt attttgtcac cggaacggat accgaagtgg ggaaaaccgt
     6301 cgccagttgt gcacttttac aagccgcgaa ggcagtaggt tatcggacgg caggttataa
     6361 accggtcgcc tctggcagcg aaaagacccc ggaaggctta cgtaatagtg acgcgctggc
     6421 gttacagcac aacagcagcc tgcagctgga ttacgcaacg gtaaatcctt acaccttcgc
     6481 agaacccacc tcgccgcaca tcatcagcgc ccaagagggc aggccgatag aatcattggt
     6541 aatgagcgcc ggattacgcg cgcttgaaca acaggctgac tgggtgttag tggaaggtgc
     6601 tggcggctgg tttacgccgc tttctgacac tttcactttt gcagattggg taacacagga
     6661 acaactgccg gtgatactgg tagttggtgt gaaactcggc tgtattaatc acgcgatgtt
     6721 gactgcgcag gcaatacaac acgccggact gactctggcg ggttgggtgg cgaacgatgt
     6781 tacgcctccg ggaaaacgtc acgctgaata tatgaccacg ctcacccgca tgattcccgc
     6841 gccgctgctg ggagagatcc cctggcttgc agaaaatcca gaaaatgcgg caaccggaaa
     6901 gtacataaac cttgccttgt tgtagccatt ctgtatttgg ttaaattgcg agcgagatcg
     6961 cgtcttcgat tgactgcaat ttaaccaatt aaattctaaa ataatcacga aaaaaatttt
     7021 acttccgcct catgcggcga atgtgggaat tgcccaggcg gcgggggata ggggctggag
     7081 acagttatcc actattcctg tggataacca tgtgtattag agttagaaaa cacgaggcaa
     7141 gcgagagaat acgcggcttg cacgcgaatt ggcgttaaag acggctcaaa gaaatatctt
     7201 ttatttttta actggttaga taaatgcaat ggcagtcact gaacaggcat ctcttgccat
     7261 aaaactgtca tcactcatct tgacaaatgt taaaaaagcc gttgctttgg ggataacccg
     7321 gtaaggctgg agttttatct cgccacagag taaattttgc tcatgattga cagcggagtt
     7381 tacgctgtat cagaaatatt atggtgatga actgtttttt tatccagtat aatttgttgg
     7441 cataattaag tacgacgagt aaaattacat acctgcccgc ccaactcctt caggtagcga
     7501 ctcatgagta aaccgttcaa actgaattcc gcttttaaac cttctggcga tcagccagag
     7561 gcgattcgac gtctcgaaga ggggctggaa gatggcctag cgcaccagac gttacttggc
     7621 gtgactggct ccgggaaaac cttcaccatt gccaatgtca ttgctgacct tcagcgccca
     7681 accatggtac ttgcgcccaa caaaacgctg gcggcccagc tgtatggcga aatgaaagag
     7741 ttcttcccgg aaaacgcggt ggagtatttc gtctcctact acgactacta tcagccggaa
     7801 gcctatgtac cgagttccga caccttcatt gagaaagatg cctcggttaa cgaacatatt
     7861 gagcagatgc gtttgtccgc caccaaagcg atgctggagc ggcgtgatgt gattgtagtg
     7921 gcgtctgttt ccgcgattta tggtctgggc gatcctgatt tatatctcaa gatgatgctc
     7981 catctcacgg tcggtatgat tatcgatcag cgcgcgattc tgcgccgact ggcggagctg
     8041 caatacgctc gtaatgatca agcattccag cgtggtactt tccgcgttcg tggcgaggtg
     8101 atagatatct tcccggcaga atcggatgac attgcacttc gcgtggaact gtttgacgag
     8161 gaagtggaac gattgtcgtt atttgacccg ctgaccgggc agattgtttc cactattcca
     8221 cgttttacca tctacccgaa aacgcactac gtcacaccgc gcgagcgcat cgtacaggcg
     8281 atggaggaga tcaaagaaga actggccgcc agacgcaaag tgctgttgga aaataacaaa
     8341 ctgctggaag agcagcggct gacccagcgt acccagtttg atctggagat gatggacgag
     8401 ctgggctact gttcggggat tgaaaactac tcgcgcttcc tctccggtcg tggaccgggt
     8461 gagccaccgc cgacgctgtt tgattacctg cctgccgatg ggctgctggt cgtcgatgaa
     8521 tctcacgtca ccattccaca aattggcggc atgtatcgcg gtgaccgggc gcgtaaagag
     8581 acactggtgg agtacggctt ccgcctgcca tcagcgctgg ataaccgtcc gcttaagttt
     8641 gaagagttcg aagcattagc gccgcaaacc atctatgttt cggcgacgcc gggtaattac
     8701 gagctggaaa aatccggcgg cgatgtggtg gatcaggtgg tgcgtccaac cggattgctt
     8761 gacccgatta tcgaagtgcg gccggtggcg acacaggttg atgatcttct ttcggagatt
     8821 cgtcagcgag cggcaattaa cgaacgcgtg ctggtcacca cactgaccaa gcgaatggcg
     8881 gaagatcttg ccgaatatct cgaagaacac ggtgagcgcg tgcgttatct tcactcagat
     8941 atcgacaccg tcgagcgtat ggagattatc cgcgacttgc gtctgggtga gttcgacgta
     9001 ttggtaggga tcaacttact gcgtgaaggt ctggatatgc cggaagtgtc gctggtggcg
     9061 atcctcgacg ctgacaaaga aggcttcctg cgttccgaac gttcgttgat ccagaccatt
     9121 ggtcgtgcgg cacgtaacgt taacggtaaa gcgattctct acggcgatag gagcactcca
     9181 tcaatggcga aagcgattgg cgaaaccgaa cgtcgccgtg agaaacagca gaagtacaac
     9241 gaggaacacg gcattacgcc gcaaggcttg aacaagaaag tggtcgatat cctggcgctg
     9301 gggcagaaca ttgccaaaac caaagcgaag ggcagaggaa aatcgcgccc gattgttgag
     9361 ccggataatg tgccgatgga tatgttgccg aaagcgttgc agcagaaaat ccatgaactg
     9421 gaagggctga tgatgcaaca cgcgcagaat ctggagttcg aagaagcggc acaaattcgt
     9481 gaccagttgc atcagttgcg tgagttgttt attgccgcat cgtaacagga tagcgaagaa
     9541 gactgatgac aaacggaaaa cagtctgatg cgctacgctt atcaggccta cattttctcc
     9601 gcaatatatt gaatttgcgc ggtttgtagg ccggataagg cgttcacgcc gcatccggca
     9661 taaacaacgc gcactttgtc ttcaatagga agccggaatt ttccttcgga tttccgttaa
     9721 cctaaagcct gtaacgcctt ttccagcgcg ttatgtaaca actggcggtc atgacgatac
     9781 ggaatatcgc tggcctccag tacctcctgg atcacaatcc gctctttcac cgccgagaca
     9841 tccacttttg gcccgacgat gaccgcatca atgacctttt taccaacata ctgctccata
     9901 attgccagct tgctttccag cttcaaatta gccgcaggta aactcaactc acgccccaga
     9961 ttgccgatat aaaccatcgg cgctggcgtg cggcgtaatg cctgggcgat ttccttcagc
    10021 agcagaattg gcatcaggct ggtataaaaa ctgccaggcc caataatgat gagatccgct
    10081 tcattgatag cgtgaaccgc ctcacgcgtt gcgggtacat taggcgttaa caataactct
    10141 tgaatcggcg tagttaactg gtcgatattg acctcgccgt aaacttcatg cccctgatcg
    10201 tcaatcgcca tcagatcaac aggatgctct gacattggaa tcaaatgcgt atccactttc
    10261 agcagattac gaattaaatt gatggcttcc agaggccgca cgctaaggtg atccagcgcc
    10321 tttaacatca agtttccgag attatgaccg gaaagttcgc cattgccgcc aaaacggtat
    10381 tcaaacatcg cggaggcgac gttcggttcc gttatcagct ggttgaggca gttgcgcata
    10441 tcgccccaag caatgccgcc ttctgaacgg cgaatacgcc ccgtcgagcc accattatcc
    10501 gtggtggtga cgatacccgt taaacgagaa cccaaagacg aaagtgatga gagaacgcgt
    10561 cccagtccat gccctccgcc gagagcaacg acacgatcaa gatcagccag cgtacgattg
    10621 cgcatatagt ttcctggagt cagattatcc gcgctacagt agcgcaaatt gcggggaaac
    10681 agcaattaac ctgccaatat tgaggataca aactattttc ttctatctct aaaggacgat
    10741 gcacgctatg cctccctgat gatgtatatc aaagtaaaac cgccattttc ccttattctg
    10801 tagcgaaata gcacgatcat gacgctatat acatgattac atagcgaaag tgtggatggt
    10861 aaaaatctca tttacacgct agtatcggct taaccactaa acactctagc ctctgcacct
    10921 gggtcaactg atacggtgct ttggccgtga caatgctcgt aaagattgcc accagggcga
    10981 aggaagaaat gacttcgcct cccgtatctg gaaaggtgta catggcttca caactgactg
    11041 atgcatttgc gcgtaagttt tactacttgc gcctgtcgat taccgatgtg tgtaactttc
    11101 gttgcaccta ctgcctgccg gatggctaca aaccgagcgg cgtcaccaat aaaggctttc
    11161 ttaccgtcga tgaaattcgc cgggttacgc gcgccttcgc cagtctgggc accgaaaaag
    11221 tgcgcctgac aggaggagag ccgtctttac gccgcgactt taccgatatc atcgccgctg
    11281 tgcgggaaaa cgacgctatc cgccagattg cggtcaccac caatggttac cgtctggaac
    11341 gcgatgtggc gaactggcgc gatgcgggac ttactggcat taacgtcagt gtcgacagtt
    11401 tggacgcccg ccagtttcac gctattaccg ggcaggataa attcaaccag gtcatggcag
    11461 ggattgatgc tgcatttgag gccggttttg agaaggtcaa agtcaatacc gtgctgatgc
    11521 gtgatgttaa tcatcaccag ctcgacacct ttctgaactg gatccagcat cgccctatcc
    11581 agctgcgttt catcgaactg atggaaacgg gcgagggcag cgagctcttc cgtaagcatc
    11641 acatctctgg tcaggttctg cgtgacgagc tactgcgtcg cggctggatc caccaattac
    11701 gtcaacgcag cgacggtccc gcgcaagtct tttgccatcc agattacgcc ggagagattg
    11761 gccttatcat gccgtatgaa aaagacttct gcgccacttg caaccgcctg cgcgtttcct
    11821 ccattggtaa actccatctc tgcctgtttg gtgaaggcgg cgttaacctg cgcgatctgc
    11881 tggaaggcga tacccagcaa caggcgctgg aagcgcgtat ttcagcggcg ctgcgggaga
    11941 agaaacagac ccatttcctg catcaaaaca acaccggtat tacgcaaaac ttatcgtaca
    12001 ttggcggcta aaacgtcaaa aggagagatc agatgagtca ggtaagcact gaatttatcc
    12061 cgacccgtat tgctattctt acggtttcta atcgtcgcgg tgaagaagac gatacctccg
    12121 gtcactatct gcgcgattcg gcgcaagaag cgggccatca cgttgtcgat gaagccattg
    12181 tgaaagaaaa ccgctacgct attcgcgctc aggtatctgc gtggatcgcc agcgacgatg
    12241 tacaagtggt attgattacg ggtggtactg gcctgacgga aggtgatcag gctcccgaag
    12301 cgctgctgcc gttgttcgac cgtgaggttg aaggttttgg tgaagtgttc cgtatgttgt
    12361 cgtttgaaga gattggcact tccacgttgc aatctcgtgc ggtagcgggc gtcgctaaca
    12421 aaacgctgat tttcgccatg ccgggttcga ccaaagcgtg ccgtaccgca tgggaaaata
    12481 tcatcgcgcc gcagctggat gcccgtacgc gtccgtgtaa tttccatcca catttgaaga
    12541 aataagtatg tcgcaactga cccatatcaa cgccgctggc gaagcgcaca tggtggatgt
    12601 ctccgccaaa gcggaaaccg tgcgtgaagc gcgggcggaa gcctttgtca ccatgcgcag
    12661 cgagacgctg gcgatgatta ttgatggtcg ccaccacaaa ggcgacgtat ttgccactgc
    12721 gcgtattgcc ggtattcagg cggcaaaacg cacttgggat ctgatcccgc tgtgtcaccc
    12781 gctgatgctc agcaaagttg aagtcaattt acaggccgag ccggagcaca atcgggtgcg
    12841 tatagaaacc ttatgccgcc tgaccgggaa aaccggtgtc gaaatggaag cgttaaccgc
    12901 ggcctccgtg gcggcgctga ccatttatga catgtgcaaa gcggtgcaaa aagatatggt
    12961 gattggtccg gtacgtttgc tggcgaagag cggcggcaag tcgggtgact ttaaggtgga
    13021 agcggatgat taaagttctt tttttcgccc aggtgcgcga gttggtggga acagatgcaa
    13081 ccgaagtggc tgcggattac ccaaccgttg aagcgttacg ccagcacctg gctgcgcaga
    13141 gcgatcgctg ggcgctggcg ctggaagatg gcaaattgct ggctgccgtc aaccagacgc
    13201 tggtgagttt tgaccatccg ctgactgacg gcgacgaagt agctttcttc ccgccggtaa
    13261 ccggaggtta agatggcaga aaccaaaatt gttgtcggcc cgcagccgtt cagcgtagga
    13321 gaagagtacc cgtggctggc ggagcgtgac gaagacggtg cggtagtcac cttcaccgga
    13381 aaggtgcgca accataacct gggcgacagc gtcaaagcat taaccctcga acactatccg
    13441 gggatgactg aaaaagcact ggcagaaatt gttgatgaag cgcgtaaccg ctggccgctg
    13501 gggcgcgtca cggtgattca ccgcatcggg gaattatggc cgggcgatga aatcgttttt
    13561 gtcggtgtca ccagtgcgca tcgcagcagt gcgtttgaag ccgggcagtt tattatggat
    13621 tatctcaaaa cccgcgcacc gttctggaag cgcgaagcca cgccggaagg cgaccgctgg
    13681 gttgaagccc gggagagcga tctgcaggcg gcaaaacgct ggtagttttt tgttagccgg
    13741 ataaggcacc gctgcgtccg gcaatttcac cgctcagtac atgcgtttac gctgcgtcga
    13801 tgcacagcct catcattttg cagtatcctt aagatattcc ttatatcttc aggagatcgt
    13861 catggacaga ttcccacgtt ctgattcaat cgtacaaccc cgggctggct tgcaaactta
    13921 tatggcgcaa gtctatggct ggatgaccgt tggcttgttg ctgaccgcat ttgttgcctg
    13981 gtatgcggct aattccgcgg ccgtgatgga gctgttgttc actaaccgtg tctttttaat
    14041 cggtctgatc atcgcgcaat tagcattggt tattgtgtta tcagcgatga ttcaaaagct
    14101 gagcgcaggt gtaacgacga tgctctttat gctttattcg gcgctgacgg gtcttacgct
    14161 ttccagtata ttcattgtct ataccgctgc ttctatcgcc agtactttcg tcgttactgc
    14221 cgggatgttc ggcgcaatga gcctgtacgg ttacaccacg aagcgcgatt taagtggctt
    14281 tggcaatatg ctgtttatgg cgttaatcgg cattgtgctg gcatcgctgg tcaacttctg
    14341 gctgaaaagc gaagcattga tgtgggcagt tacctacatc ggcgtgattg tctttgtcgg
    14401 attgacggcg tatgacactc agaaattgaa aaatatgggt gagcagattg atacccgcga
    14461 cacgtcgaac ctgcgcaaat attccattct tggtgcgtta accttgtatc tggacttcat
    14521 caacctgttc ctgatgttgt tacggatctt cggcaaccgc cgttaattct ttttcaccat
    14581 cactatcccg gccatgtgtc gggatttttt tcttactaaa aacgacactt aacaagtacc
    14641 aggtataaaa tatgccttat gagcgagcgg aatcgttttc cgtatccata aatacaaaag
    14701 tattttgtat gcgtggtttt aaatttattg atggtgaata ttaatgtttt ttctaaggac
    14761 aactaaaaga atggaatcat actcgcaaaa cagcaataaa ttagattttc agcacgaggc
    14821 caggatatta aacggtatat ggctcattac cgctttaggt ttggtggcaa ccgcaggact
    14881 agcctgggga gctaagtatc tcgaaattac ggcaaccaaa tatgattcac caccaatgta
    14941 tgtcgccata gggttattat tactttgtat gtatggctta agtaaggata tcaacaagat
    15001 aaatgccgcc atcgcgggcg taatatatct gtttttactc tctttggtgg cgattgtcgt
    15061 tgcaagttta gttcctgtat atgccattat catcgtgttc agcactgcgg gcgcgatgtt
    15121 tttaatcagt atgctggccg gtttattatt taatgttgat cctggttctc accgttttat
    15181 cattatgatg acgttgacag ggttggccct ggtaatcatc gtgaatgcgg cattaatgag
    15241 tgaacggccc atttgggtaa taagttgctt aatgattgtg ttatggccag gcattatctc
    15301 gcatggacga aataagctcc ttgaacaggc aggaaaatgc catagtgaag agttgtggag
    15361 tccggttcgt tgcgctttta caggtgcatt aacactctat tactatttta tcggcttctt
    15421 tgggatactt gccgcgatag ctataacgct tgtctggcaa aggcatacgc gtttttttca
    15481 ttagtcgtaa atgcagatac taaagaactt aactaaactt cacatcgccg cttcattttt
    15541 cgcccgtagc ttcttcgctt ggctttccag tagcaggtaa cagatcagcg ccagcagcag
    15601 cgggataaag taatacagga cgcgataagc gagcagggcg gcgataatgc tccctttcga
    15661 ggtatgctcc ccagccagta gtgcgataaa caccgcttcc agcacaccga tccccgccgg
    15721 aatatgcacg atgacgccag caatactact aaccagtaac acgcccagta caaagaaata
    15781 gttcacgctt tgaccaagta acagccagat aatcgccccc attaccatcc agttaacgct
    15841 ggaaatcagc atctgagcga gggcgaattt ccatgaaggc agcaccagct tttgcccttt
    15901 gatggtcata tggcggtgct tcgcgaaagc gcaaaaccac aaataaaccg cgataatcat
    15961 cagtaagcca atgccgagaa tacgcagcgt agtttgatcg acataccagt aatccggcaa
    16021 ctccaccacg cctgcggtaa agataatccc tgccagtaaa atatagccca gccagttggt
    16081 ggtaatactg agcgaggaaa tccgcgtaat agtgctgccc ggtaacccta gtcgggagta
    16141 caaacgatag cgcataccaa tgccgccgac ccaggtactg agcgtcaggt tgaaggcgta
    16201 gcagataaac gacaccagca tcacctggcg cttcgccagt ttgtgaccgc agtaaaagcg
    16261 ggcgagcagg tcatagcagc cgtaaatcag atagctgacg accaccagcc cgaccgcact
    16321 aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc agtccacttt
    16381 tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg tgaggatctt
    16441 ctttgctaag cgccagcgcg ggtgtgattt actcatcagg ggtttacccc cgtgttttca
    16501 gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg ggggatcaac ctgcgtcaga
    16561 cgtggtgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa aaagtggaac
    16621 gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt ttcatccacc
    16681 tgctgacaat ctgcggcaat aatgccgttc agattatcgc gcagcgtctg gttaaaatga
    16741 cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg atcgagatta
    16801 ctggacccta ctgtcgccca gtgatcgacc atcaatgcca ctttgccgtg gagcgggcgg
    16861 cggcggtact caaaaacctg aatgccgcct ttaaccagat agttatacag caagcgcgca
    16921 ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat ccgcaccccg
    16981 cgccgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa gtaggcgttg
    17041 gcgataatca cttcccgctg cgcctgagtg agcattttca gataatggcg ttcaatatca
    17101 tcgcgatgtt cttcgttatc gcgccagacc agcaatacct gcgcttctcc cggctggcgg
    17161 ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg tccaggcagg
    17221 ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg taccgcgtaa
    17281 tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt cagcccgcca
    17341 ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg aaacacattg
    17401 gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac gccagctgcc
    17461 gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc cagcaagact
    17521 tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg tttgccgacg
    17581 ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc gccaatcgcc
    17641 ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt attgccttcg
    17701 cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca gaaaggtgtc
    17761 gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg acgtagatcc
    17821 tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt ccgtgggcgc
    17881 gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct ttttgccgcc
    17941 agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca ttcacccatt
    18001 cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga tgtacgcaca
    18061 tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc acgccgcgct
    18121 tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga taacgcgaca
    18181 gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag gcaaaatcgc
    18241 tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt tccacatgca
    18301 gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg gcgctgacgg
    18361 tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg gtaaagcctt
    18421 tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg ggcataattt
    18481 tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa aaggtgcggt
    18541 cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat tcttcaggag
    18601 agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt gctggcagat
    18661 tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta agactctggt
    18721 tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga tgctggtgtt
    18781 tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag agctgaccac
    18841 gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg tactggaaaa
    18901 caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg tagtgtcttt
    18961 gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac gccgctgacc
    19021 ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata tccagactcg
    19081 catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg ttaatccacg
    19141 tcagattttg cagccacact ggcatgtttt ccaccggcga aacgtaaccg gaaaggagaa
    19201 tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc gagcagagtg
    19261 atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc gtaaagtaga
    19321 acagcgctag cgatccggcg aaggggattt gatacgccca gataccaatc gccagcacaa
    19381 tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg aagatctgcc
    19441 aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt tcgcgggcga
    19501 cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc agcgacggca
    19561 ccacaaacca tttgtagtcg agattcgggt tataccagtt gcgtaccacc agctcgctgt
    19621 tgttgggttt cggttttcct tccagcagct cctgctgata atttttgacg atctgttgca
    19681 ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc aactgcaacg
    19741 gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc accagtagta
    19801 acgccttttg tgtgtcgatg gtcgggcgga tctcctgtgg gcttttcagc agcagcacat
    19861 gtgtaaaggc gctggcgcgg gcaaaacgtt gagtaagttc caccgaatgc tcgccgttat
    19921 cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg aacgggaaca
    19981 ggatcacctg aattagcacg ggtaaaatca gaatcgcgcg ggtttgtggt tcgcgcagca
    20041 acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc ttctccctaa
    20101 tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc cgaagcgatc
    20161 aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa caggctttgc
    20221 agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat caccgcgggc
    20281 atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcagaaa agcggcgttc
    20341 agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag cagccccatt
    20401 cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaatcagcag cgaaccgcga
    20461 tacggcacgc cgagaataaa cactgacacc agcatacaca gcaacatcgc cagcatcccg
    20521 agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat ctccgtagag
    20581 agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga ggtgagaatc
    20641 gcgccgatga ccgtcatgat aatggtcacc gcacccggga taataaagtg ctggcttatc
    20701 gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa agtctgcccg
    20761 ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata cccctgcaca
    20821 aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc ggtggcgttg
    20881 gcgcgctcca tctgttcggc gaaatccacg ggaataacca ccagaccgcg aattttcccc
    20941 gactgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc gatgtagggc
    21001 gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc cagcaaaatc
    21061 ccgacccgca gcttgctgga gtcgaggtta atgccgtaac caaaaataaa cagcagtagc
    21121 agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg ccgcgtctct
    21181 ttgacgcaca gcgcccgcac gcgatgccag gaaaggatcg ggttactcat tgctatgctc
    21241 cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat cgggttgctc
    21301 atcgttagct gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta atttcccacg
    21361 gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg tggtgaccat
    21421 caccgtgacg cctttctcta ccatgctgtt gatgtgcagc caaaattcac ggcgggtgag
    21481 ggggtcaacg ccggaagtcg gttcgtcgag aaagagaata tccggttcat gcatcagcga
    21541 acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg cgtgtgaggc
    21601 aatacttttc aggccaaatg cctcgctcat acgggagatt ttttcgttct gcgcccgacc
    21661 gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca ggttgccgta
    21721 gagcgaaaat ttttgcgcca tatagccgag atgctggcgc gctttaccgg aactctcttt
    21781 cagatccatc cccagcacca gcgcctggcc ggaagtcggc accagcaaac cgcacatcat
    21841 cttaaaggtg gtcgatttac ccgcgccgtt tggccccagc aaaccaaaaa tctccccacg
    21901 tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct tggtcagttc
    21961 tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgcaata ttgcacccag
    22021 cggcgattct gaggttccgg caccgcccag caaatcaata aacgcatctt caaaacgcgg
    22081 cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat cgtctggtgt
    22141 ggcctctttt ttgaggatca gacgtaccga tttcccctga atcatgccat cgctgacctg
    22201 cggcagtttc aaggcgcgtt gcaacagttt gcggttgccc tcgtgtggac tggtcatcag
    22261 aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tctccctgat acagcaactc
    22321 gccttcgttc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat acgaggtact
    22381 ccagaggatt aacatccctt cgcccgccag ctcatgcacc atctgccaca gttcgcgccg
    22441 tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg gttcgcccac
    22501 cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc ccgccaggcg
    22561 tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt gcttacgtgc
    22621 ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca ccgtgagatc
    22681 ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg cgccgtcgtt
    22741 tttgatcgga tcaaagccaa tcaccgtggc actgccgctg tcgggtttca gtaatcccgc
    22801 caacatccgc atcagtgtgg ttttacctgc accgtccggc cccaccaacc ccgtcacata
    22861 accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc ccggaaagcg
    22921 tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg tcaccgaatt
    22981 gtaccgtcac tggcattccc tggcgtaacg catcatcggc gtcggtcacc acaatacgca
    23041 ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg gtaaattcag
    23101 cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga ccatcggtat
    23161 aaagcagcac tttgcgcccc ggctgggcct ggtcaagatt ccgttcatca acataagcgc
    23221 gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca ttgagaaccg
    23281 tgcctggctc taccgcgcgc gttaacagcg tgccatcaga cggtgctatc aacgttgagt
    23341 cctgtaaatt cagctccgcc tgcgccagtt gcgcctgcgc ctgttcgagg ctggctttcg
    23401 cctgagcgat gtcctgttca cggttaccgg aacggtactg acgcaattta tcctgtgctg
    23461 atttcagcgt tgcctgtgcc tggtcgcgcg aggagcgggc attttccagg tcatttgccg
    23521 aaatggtgcg gcttttccac agtccttgct ggcggttata gaagttctgc gcatagtcat
    23581 aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca tcgcgatacc
    23641 cggcaagcat cagattatac tgcgcctgtg ccaccgagac gcccgctttc gcctgcatca
    23701 gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct ttgatagcat
    23761 caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta agattcaccg
    23821 tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt tgataccacc
    23881 agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagtccgatc acgacaggtt
    23941 ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga tatgacaggt
    24001 caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc cggtacgtaa
    24061 cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg catgggtatg
    24121 aaggatcatg cgggtgtcat tagcgtcgca gccggtccag gcggcaatca ggcgtgtcag
    24181 gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg ccgtgggaga
    24241 gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg tgagcagctt
    24301 aatcatgttc ctgcaggcgc gaaggatcag ttcacggatg gcagcccgat caggctgtgg
    24361 ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc caataaaatc
    24421 ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt agtaggtgat
    24481 ggcagcgata ttctgcccgg cctgggcggc tatctcgcgg gtggtggcgt tcattccata
    24541 ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt cacccttgat
    24601 tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg attaagatta
    24661 tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca gtcacaggga
    24721 taatttatgc gctgcgtcac aaaaactgct acactccgct ccctcatgac attgtggttt
    24781 ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc ggggcggttc
    24841 ggagtagtta tgtctttcga ttctttgggt ttaagccctg atatcctgcg cgccgttgcc
    24901 gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc ggtgctggaa
    24961 ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg ctttacgctg
    25021 ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg tccggtacgt
    25081 gcgctcattc ttaccccgac ccgtgaactg gcggcacaga ttggcgaaaa cgtccgtgat
    25141 tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gcggtgtcag cattaaccca
    25201 caaatgatga agttgcgcgg cggcgttgat gtgctggtgg caacgccggg acgtttgctg
    25261 gacctggaac atcaaaatgc agtgaagctg gatcaggttg aaatcctcgt cctcgatgaa
    25321 gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt aacaaaactg
    25381 ccagcgaaac gccagaacct gctgttctct gcgaccttct ctgacgatat taaagccctg
    25441 gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa taccgcgtct
    25501 gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga attgctgtcg
    25561 cacatgattg gtaaagggaa ctggcagcag gtgctggtgt ttacccgtac caaacacggc
    25621 gctaaccatc tggctgagca gctcaataaa gatggcatcc gtagtgtggc gatccacggg
    25681 aataaatcgc aaggtgcgcg tacccgtgcg ctggctgatt ttaaatcggg tgacattcgc
    25741 gtactggtgg cgaccgatat cgctgcgcgc ggtctggata tcgaagagtt gccgcacgtc
    25801 gtcaaccatg aactgccaaa cgtaccggaa gattatgtcc accgtatcgg gcgtaccggt
    25861 cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca caaactgctg
    25921 cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc gggctatgag
    25981 ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg tggcggcatc
    26041 aggctgatga acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc tacgtgaact
    26101 ctgcaatata ttgaatttgc atgcttttgt aggccggata aggcgtttac gccgcatccg
    26161 gcatttcaca acaagcactt gtcagcaatt tgagaacacg ggaaaaaatt tatctgtttt
    26221 accgcccata agccaccgag acgttacttc tctatagcca attgttcgcg caactccatt
    26281 aaaaggtagc ccagtcgatt cttgccctta ccattaccac cgtctcccca gtaagcatcg
    26341 ttttccgtat gctcaaccag ttttgcgggc gcggttgcca gcaagagcac acgcagttct
    26401 gcatgctgtt cgaatttagc gcgaagtgct ttacgcatca cctgttcttt aaccgactcc
    26461 cagtttttac gaagtggctt ggaacgatta cgccccatgc gagcggcgac catgggggaa
    26521 gaaacccgac ggatctcttc gcggtatttt tcatcaagga atttttgtgc ctgaaaatag
    26581 tgttcggagg tgggccaggt attcccgtca accttgatgg gcctggcggc aaagttagaa
    26641 aaatcaccgt agtcatcgct ggtgctgtag aaatttatga tggtgtcttg catgacgtgt
    26701 tggatccttt gtgctcgaac gggcattaaa ccgcattatg ttggtggtta ttgcgagccg
    26761 ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattggctg
    26821 tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc
    26881 cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg
    26941 gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat
    27001 tgaagccccc accggcgttg ggaaaacgct ctcctatttg atccccggca tcgccattgc
    27061 ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcgttgc aggatcagat
    27121 ttacagcaaa gatttaccgc tgctgaaaaa gatcattccc gatcttaaat tcactgccgc
    27181 tttcgggcgt gggcgctacg tttgtccacg taatctgacg gcgctcgcca gcactgaacc
    27241 cacgcaacag gatctgctgg cgtttcttga cgacgaactg acgccaaata atcaggaaga
    27301 gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg atggtctgcg
    27361 cgatcatacg gatatcgcta tagatgatga tctctggcgt cgtttgagta ccgacaaagc
    27421 cagctgcctc aaccgtaact gttactacta tcgtgaatgc ccgttttttg ttactcgtcg
    27481 ggagattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga
    27541 aagcgaagcc gtactgcctg acccgaaaaa tctgctgctg gtgctggacg aaggtcatca
    27601 cctgccagat gtggcccggg atgcgctgga gatgagcgct gaaatcaccg cgccctggta
    27661 tcgactacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc agtttcgccc
    27721 gaagaccatc ccaccgctgg cgatccctga acgtttgaat gcgcattgtg aagagttgta
    27781 tgagcttatc gcctcgttaa acaacattct caatctctac atgcctgccg ggcaggaggc
    27841 agagcaccgt tttgcgatgg gcgaactgcc tgatgaattg ctggagatct gccagcggct
    27901 ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga
    27961 gaaaaccggc agccacgata ttgtgcgtct gcatcggttg attttacaga tgaaccgcgc
    28021 gctggggatg tttgaggtgc aaagcaaact ctggcggctg gcgtctctgg cgcaatcttc
    28081 cggtgcgccg gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc tacacctctg
    28141 gtttcactgc gtgggaatac gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat
    28201 tccgcacatt attgtcacct ccgcaacctt gcgttcgctg aatagttttt cgcgtttgca
    28261 ggagatgagt ggtctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt
    28321 taaccactgc gaacagggca aaattgttat tccccggatg cgctttgagc cttccatcga
    28381 caacgaagag caacacattg ccgaaatggc ggcctttttc cgtgaacagg tggagagcaa
    28441 aaaatatctc ggtatgttgg tactgtttgc cagcggacgg gcgatgcagc gctttctcga
    28501 ctatgtgacg gatttacgcc tgatgttgct ggtgcagggc gatcagccgc gttatcgctt
    28561 agttgaactg caccgcaaac gcgttgccaa cggtgagcgt agcgtgctgg tgggcttaca
    28621 gtcatttgcc gaagggcttg atttgaaagg tgatctgctc agccaggtgc atatccacaa
    28681 aatcgctttt ccgcccatcg acagcccggt ggtgatcacc gaaggggaat ggctgaaaag
    28741 cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc gcctcgttta acctgattca
    28801 gcaggttggg cgactgattc gaagccacgg ttgctggggc gaagtggtta tctacgacaa
    28861 acgcttgctg acaaaaaatt atggcaagcg actactggat gcattaccgg tatttccgat
    28921 agagcaaccg gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg
    28981 ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat
    29041 cattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg
    29101 cggtctgtat gctcatatgc tcaatggtga agtccctgac cttgagttgg gcggcgtatt
    29161 gattgcgctg cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat
    29221 gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc
    29281 cagttacaac ggcgcgcgta aacaggctaa cctgacgcca ttattggcga ttctgctgca
    29341 taaactcggt tttcctgtgg tggttcacgg agttagcgaa gatccaactc gcgtgctgac
    29401 agaaaccatt ttcgaactga tgggtattac accaacgttg cacggcgggc aggcgcaggc
    29461 gaagctcgac gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga
    29521 aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa
    29581 actggcgacg ccatttgccg aaggtgaggc actgtgtctt tccagcgttt cgcatccgga
    29641 atacattgga cgcgtcgcga agttctttag cgatatcggc gggtgggcgt tactaatgca
    29701 tggtactgaa ggcgaagtgt atgctaatcc gcagcgctgt ccgcagatca atctcattga
    29761 ccgtgaaggg atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg agttactgcc
    29821 acaagcaaaa gatccggaaa ccacggcgca gtggactgag cgttgcctgg ctggcagcga
    29881 agcgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggtgaagc
    29941 ggcaactatc agcgatggcc tggcgcgcgt taatcaggca ttttaatttt tatctcagcc
    30001 ggatgagcca cgtcatccgg cttttacccc accgcaatat gaaattcctg catctttatt
    30061 gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag
    30121 ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagttt tattcaggct
    30181 gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagttgccga tcatttaatc
    30241 gcggcaaacc tggcagggca tgattctcat ggtattggca tgatcccaag ctatgtgcgt
    30301 tcctggagtc aggggcatct gcaaattaac catcatgcca aaatcgttaa agaggcgggg
    30361 gcggcagtca cgctcgatgg cgatcgcgca ttcggtcagg tcgcggcaca cgaagcgatg
    30421 gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct acataactcg
    30481 catcatatcg gccgtatcgg ttactgggcg gagcagtgtt cagcggcggg gtttgtctct
    30541 atccactttg ttagcgtggt cggtattcca atggtcgcgc cgttccacgg tcgcgacagc
    30601 cgctttggca ccaatccctt ctgtgtggtt ttccctcgta aagataattt cccgctgttg
    30661 cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc
    30721 gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg
    30781 gtaatgcagg agtcgccgtt ggggtcgctg ttgacctttg ccgaacataa aggctacgcc
    30841 cttgcagcga tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac gacgcatcag
    30901 gaaacgttgc aaaccagccc cgatgccatt cttaactgca tgaccactat catcatcaac
    30961 ccggaactgt tcggcgcgcc ggattgtaac gcgcagaccg aagcctttgc cgagtgggtg
    31021 aaagcctcgc cgcatgatga cgataagccg attttgctac cgggcgagtg ggaagtgaac
    31081 acgcgtcgcg aacggcagga gcaggggatt ccattggatg cgggaagctg gcaggccatt
    31141 tgtgatgcgg cgcggcagat tggtatgtcg gaagagacgt tgcaggcttt ctgtcagcag
    31201 ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat
    31261 caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga
    31321 cgtagcgtaa acgcctgatc cgtccaacgg ttcggatttt gtaggcctga taagacgcgg
    31381 tagcgtcgca tcaggcatct gttgccggat gcggcataaa cgccttatcc gtcctacgaa
    31441 ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg aagtgatgct
    31501 gtatccactg gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag
    31561 ggtggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc
    31621 cgttaccggt tccgccgcga acacgccaaa tgacagagtt gaaagagcca tagcagcaac
    31681 aacagtattg atagttttca taattaatct ctcgcaggtg atttttgtat aagtgacgtt
    31741 gtttcgtcga tgtgatgagt atcacgtttt tttacgagag ataaaatcga agagaattga
    31801 ccgccttgtt caaataaatt gattgatatc taacatactg ataaaccatt tatttgttgt
    31861 acttatggac ttgttttatg ccgcgtggca gtcacagcag cgttaaaggc acggaatgcc
    31921 acgcaaaata caaattatac tcgctatgca tctcgccagt tgtagttaac gtaactgtct
    31981 gtctttcgaa cctctgcgat tatatcctgt atacgctggt ttttgtaaat ctttctcctg
    32041 ctgacaatga atacataagc gcacgccagg aatggcttcc cgacgggctt gcgggatggg
    32101 ggcaccgcac tcttcacatt catacaggct ttcgccacgc ggaatttcac cccgagcgcg
    32161 ggcaatcgca tcttcaattg tactgttgat ctgttcgttg acggcgtcat cgttagccca
    32221 agcggatgcc atatcgtcct ccccatatca atgcttgtac agttaagtgt agctaatata
    32281 gggacgaacc tgggcagttc aagcgtcaga tctccgacca ttcccgcagc agattatgat
    32341 aaagattgag cagcgacagg atctcttcat tttcaccata gcggcttttc agcgactgaa
    32401 tatttttgtc cagttcaaac agcatggcgc gctttttatc atcgcggatc atcgactgga
    32461 tccacataaa tgatgccact cgtacgccgc gggttacggg tgtcacgcaa tgcaggctgc
    32521 tggagggata caacaccaga tcacctgccg ggagttttac ccgatgttgt ccaaaggtgt
    32581 cattaacgac cagttcgccg ccgtcgtagc tttgtggatc gcttaaaaac agcgtggcag
    32641 aaaggtcagt gcgcatccag ccgttttgtg gatggctgcg tactgcgcca tccacatgga
    32701 aaccataggt ttcattgttc tgatagcgat taaacagcgg cgtggaaagg gtacgcggca
    32761 aggcagcggc aaagaacaat gcatgttggt taaccgcgtt cagcacctca ttttgcagtg
    32821 cggcgtataa cgcgctgcgg gtgtctactt gctgattgtt cttaacttgt gcaccttgtg
    32881 ctccggtggt gacgcgtcca tccacccatt cggcttgttc cagttgttcg cgaaaatggg
    32941 cgacgtcctg tggcgataac acgccgggaa tgtggtacat catacaagtt ctccaaaaag
    33001 tggggcctgc gccccacatc tgaatcagaa atgcatattg gctgtgagca agaaggttct
    33061 tggctcgccc gggtgataac ggtagccgct cttgttgatt gaggcgacgt aatcggtatc
    33121 aaacaggttg taaacgttta gctggaagtc gagattgcga ttaacccgat accccagttt
    33181 ggcatcggcg acccagtaac cttctgtaaa tgctggcgtt cccaccgcgc cgtctgaacc
    33241 tttatgtata ctgccgatat agcgtgcgcc cgcgccaaca gagatatcat cggttgcctg
    33301 atattggctc cataaggtga aggcgtgctc cggggtatac ggcagcgatg aggaaccatc
    33361 ctgggcaaca tctttgccgt ttttgatggt tgctttttgc tgggtatagc cgccaatcac
    33421 ctgccacgcg ggagtgatat tccctgccac ggatatctca tagccttcga cgcgtttctt
    33481 accgtattgc gagtaagtcc cgtcatcatt ttgctcaact tcattttcga tatcagtacg
    33541 gaacagcgcg gcggtgagca acaggcgttt atccagaacc tgccatttgg tgccaatctc
    33601 gctggtgttg gctttttgcg gtttaaaatc ggtgcggttg gcactgttac cactgccaga
    33661 ctgcgcaagg gcgaagttgt tgccgcccgg aggctgctgg gaaacggcat agttaatata
    33721 gacattgccg ttttccgtca ggtggtacag cgccccggct ttccagttca ccagattgcc
    33781 cgacttggcg gtgtcgacgg tggtgactgg agaacctttt gccacaccag ctgggcaggt
    33841 gatggcaccg cgtccgctgc cgccgcaggc ggtggcactg tcatattcag tatgataatt
    33901 atccagacgg atcccgccgt tcagctcaaa atcacgggtg atttgcagcg tatcgaaggc
    33961 gtaaattgcg aaggtatccg tctgaccatt ggcgtttgcg ccgttgcgcg tcaggccgcc
    34021 gggatgaatt ctgctgtcag gatgataaat atttaccgcc ggtaacgtca ccggattaac
    34081 gccatagtta gtctgcgttt cacgggtaaa ttccacgccg gtactgacat catgaccgat
    34141 agaaccggta tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt tattactcac
    34201 atctttggta ttcgccgtgc gtgactaggt ccagctattc acatcgctgg tgggctgagt
    34261 aatattcgac gccccgccca taatcgccgt catcaggtaa tcctgcttta cgcgcgacca
    34321 acgggtagta ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac gcatggtggc
    34381 ggtgtcggtg gtcgaatcgt cgtaatcgga atccgtgccg taaaagttat gagtatcaac
    34441 ttttccggaa tgattcaggg ccgccgctcc cgcagatggg gcagaatagc ccggcaaacc
    34501 gatggtcgga atgccgccgt ctggcgtgtt gtgctgagtg acatgcagat aattaagata
    34561 caaacgattt gctgtaccaa ggccaaaagc gacagaaggg gcgacgccgt aacgctcatt
    34621 tttgactttg tcgcgtccgg catcgtgggt tttttcgccc atcacattca ggcgcaccgc
    34681 ggtagtatca ccaatgacct gattgacgtc cagcgtgccg cggcggaacc aggcgctgcc
    34741 aatactggcg gaggcgtcaa tgccggaatc attgcgcggc tgcttgctga tcatattgat
    34801 cgagcctgtc ggtgcgctgc gcccgtagtc ggtgccggac ggccctttaa tcacttcgac
    34861 ctgctcggta ttgaaggtgt cgcgcgagac gctgccgata tcgcgaatac catcaatata
    34921 aatactgtta gaggtatcgg caccacgcat ataaatggcg tcgccagtgg tggagttacc
    34981 gttctcaccc gcaaaaaacg cacccacgcc gg
//
LOCUS       DAECNO010000035        34658 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.035, whole genome shotgun sequence.
ACCESSION   DAECNO010000035 DAECNO010000000
VERSION     DAECNO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.035"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1128)
                     /locus_tag="KKO08_001748"
     CDS             complement(<1..1128)
                     /locus_tag="KKO08_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBD6318024.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVGNAANLLI"
     gene            complement(1340..1687)
                     /gene="pspE"
                     /locus_tag="KKO08_001749"
     CDS             complement(1340..1687)
                     /gene="pspE"
                     /locus_tag="KKO08_001749"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBD6318025.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1730..1951)
                     /gene="pspD"
                     /locus_tag="KKO08_001750"
     CDS             complement(1730..1951)
                     /gene="pspD"
                     /locus_tag="KKO08_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBD6318026.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1960..2319)
                     /gene="pspC"
                     /locus_tag="KKO08_001751"
     CDS             complement(1960..2319)
                     /gene="pspC"
                     /locus_tag="KKO08_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBD6318027.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2319..2543)
                     /gene="pspB"
                     /locus_tag="KKO08_001752"
     CDS             complement(2319..2543)
                     /gene="pspB"
                     /locus_tag="KKO08_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBD6318028.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2597..3265)
                     /gene="pspA"
                     /locus_tag="KKO08_001753"
     CDS             complement(2597..3265)
                     /gene="pspA"
                     /locus_tag="KKO08_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBD6318029.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3432..4409
                     /gene="pspF"
                     /locus_tag="KKO08_001754"
     CDS             3432..4409
                     /gene="pspF"
                     /locus_tag="KKO08_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBD6318030.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4529..5794)
                     /gene="puuE"
                     /locus_tag="KKO08_001755"
     CDS             complement(4529..5794)
                     /gene="puuE"
                     /locus_tag="KKO08_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBD6318031.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5832..7112)
                     /gene="puuB"
                     /locus_tag="KKO08_001756"
     CDS             complement(5832..7112)
                     /gene="puuB"
                     /locus_tag="KKO08_001756"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBD6318032.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7114..8601)
                     /gene="puuC"
                     /locus_tag="KKO08_001757"
     CDS             complement(7114..8601)
                     /gene="puuC"
                     /locus_tag="KKO08_001757"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBD6318033.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8751..9308)
                     /gene="puuR"
                     /locus_tag="KKO08_001758"
     CDS             complement(8751..9308)
                     /gene="puuR"
                     /locus_tag="KKO08_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBD6318034.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9335..10099)
                     /gene="puuD"
                     /locus_tag="KKO08_001759"
     CDS             complement(9335..10099)
                     /gene="puuD"
                     /locus_tag="KKO08_001759"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBD6318035.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10311..11729
                     /gene="puuA"
                     /locus_tag="KKO08_001760"
     CDS             10311..11729
                     /gene="puuA"
                     /locus_tag="KKO08_001760"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBD6318036.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11878..12042
                     /locus_tag="KKO08_001761"
     CDS             11878..12042
                     /locus_tag="KKO08_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318037.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12032..13417
                     /gene="puuP"
                     /locus_tag="KKO08_001762"
     CDS             12032..13417
                     /gene="puuP"
                     /locus_tag="KKO08_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBD6318038.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13551..13796
                     /locus_tag="KKO08_001763"
     CDS             13551..13796
                     /locus_tag="KKO08_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBD6318039.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14109..15752
                     /gene="sapA"
                     /locus_tag="KKO08_001764"
     CDS             14109..15752
                     /gene="sapA"
                     /locus_tag="KKO08_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBD6318040.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15749..16714
                     /gene="sapB"
                     /locus_tag="KKO08_001765"
     CDS             15749..16714
                     /gene="sapB"
                     /locus_tag="KKO08_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBD6318041.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16701..17591
                     /gene="sapC"
                     /locus_tag="KKO08_001766"
     CDS             16701..17591
                     /gene="sapC"
                     /locus_tag="KKO08_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBD6318042.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17591..18583
                     /gene="sapD"
                     /locus_tag="KKO08_001767"
     CDS             17591..18583
                     /gene="sapD"
                     /locus_tag="KKO08_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBD6318043.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18585..19391
                     /gene="sapF"
                     /locus_tag="KKO08_001768"
     CDS             18585..19391
                     /gene="sapF"
                     /locus_tag="KKO08_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBD6318044.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19459..19812
                     /locus_tag="KKO08_001769"
     CDS             19459..19812
                     /locus_tag="KKO08_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBD6318045.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20180..20968
                     /gene="fabI"
                     /locus_tag="KKO08_001770"
     CDS             20180..20968
                     /gene="fabI"
                     /locus_tag="KKO08_001770"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBD6318046.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21112..22239
                     /locus_tag="KKO08_001771"
     CDS             21112..22239
                     /locus_tag="KKO08_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBD6318047.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22307..24241
                     /gene="rnb"
                     /locus_tag="KKO08_001772"
     CDS             22307..24241
                     /gene="rnb"
                     /locus_tag="KKO08_001772"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBD6318048.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24319..24421)
                     /locus_tag="KKO08_001773"
                     /pseudo
     CDS             complement(24319..24421)
                     /locus_tag="KKO08_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24475..26449
                     /gene="pdeR"
                     /locus_tag="KKO08_001774"
                     /pseudo
     CDS             24475..26449
                     /gene="pdeR"
                     /locus_tag="KKO08_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26597..26770
                     /locus_tag="KKO08_001775"
     CDS             26597..26770
                     /locus_tag="KKO08_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318049.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26860..27609
                     /locus_tag="KKO08_001776"
     CDS             26860..27609
                     /locus_tag="KKO08_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6318050.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27878..28096
                     /gene="osmB"
                     /locus_tag="KKO08_001777"
     CDS             27878..28096
                     /gene="osmB"
                     /locus_tag="KKO08_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBD6318051.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28222..28548)
                     /gene="yciH"
                     /locus_tag="KKO08_001778"
     CDS             complement(28222..28548)
                     /gene="yciH"
                     /locus_tag="KKO08_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBD6318052.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28548..29285)
                     /gene="pyrF"
                     /locus_tag="KKO08_001779"
     CDS             complement(28548..29285)
                     /gene="pyrF"
                     /locus_tag="KKO08_001779"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBD6318053.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29478..30647)
                     /gene="lapB"
                     /locus_tag="KKO08_001780"
     CDS             complement(29478..30647)
                     /gene="lapB"
                     /locus_tag="KKO08_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBD6318054.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30654..30962)
                     /gene="lapA"
                     /locus_tag="KKO08_001781"
     CDS             complement(30654..30962)
                     /gene="lapA"
                     /locus_tag="KKO08_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBD6318055.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31111..31875)
                     /gene="pgpB"
                     /locus_tag="KKO08_001782"
     CDS             complement(31111..31875)
                     /gene="pgpB"
                     /locus_tag="KKO08_001782"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBD6318056.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32045..32635
                     /gene="ribA"
                     /locus_tag="KKO08_001783"
     CDS             32045..32635
                     /gene="ribA"
                     /locus_tag="KKO08_001783"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBD6318057.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32699..>34658)
                     /gene="acnA"
                     /locus_tag="KKO08_001784"
     CDS             complement(32699..>34658)
                     /gene="acnA"
                     /locus_tag="KKO08_001784"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBD6318058.1"
                     /translation="QPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGK
                     FVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVE
                     KYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNEL
                     EVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTL
                     GLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPI
                     ETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPI
                     GHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTY
                     GWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPA
                     GRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDV
                     VSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSN
                     LIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPC
                     RCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8172 a         9037 c         8750 g         8699 t
ORIGIN      
        1 aatcagtaag ttggcagcat tacctactaa tgcaccttcc tgctgtaatg cctcgaccag
       61 cgctaatatt tcgctttctg gaagcaagcc acgcagtggg ttcaacccga tgccatcgtg
      121 agaggcgagg aagttaaacc aggtcgtttt actggagggc aagctcagac tttgcgccca
      181 ctggcacagc gcctcgacgt tctgtttttg caccgcatgt aacaccagcg gcggcagcga
      241 gaactggtac accatatgcg cttcgtcatc accgttgcca aagtaagcaa tgttgtcttt
      301 gtgcggaaca ttggtttcgg taatgatcac tgtacctggt gctacgtcat caataatcga
      361 ccgtaacagc ttgataatca gatgtgtttt ttccagatgg atgcagcttg ttcccggctc
      421 tttccacata aagccaacgg catccaggcg gacatactca gcacctttct caaggtaaca
      481 gagtaaaaca tccaccatcg ccagcaacac ttcagggcta cggtaattca ggtcaatttg
      541 atcgtcacta aaggtggtcc ataaatggcg cgtagaatta tcatccattt gaaacggcgt
      601 taatagcggt aacgcacgcg gaagggtgac ggcactgaga tcggttttcg gatcaaccgc
      661 aataaagaaa tcttcaaaac ccggaagctg ttgtaaatag tttttaaacc attcactttt
      721 tgcagacata tggttgcaga caaaatcaaa cattaaatga ctgcattcac cgagttgctg
      781 aatatcctgc cactcccccg cttcactggc gacctgatga taatcaatta ccgaaaatcc
      841 atcatcagat gaccatgggt aaaaaggtaa taaatgaaca tgtgaaaaaa tgctttgcag
      901 ccattgatgg tagaactgat taaatatggg tagtggtttt aaatcattac tgtgaaattg
      961 atcggcatag gtaattaaca cgacatcact ttcatcccag tgttttttac gtcgctgtgt
     1021 aattaatcgt ttcgcactct caagacgcgt tacaagtttc tgtaaatgag ttgcggtaaa
     1081 tgttccaccg tagatttcgt ccatgtaatc cgtaattttc tgtttcatgt tgcctttccg
     1141 cggtagcggt cccatgaggc gagagactac tccgcagaaa ataaactgtc aattgagcac
     1201 aagggtttta gtcacgcagg aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg
     1261 acgcaaataa tctataggga tgtaaataag gtgtgacaga aaaaaaacgg cgcataagcg
     1321 ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct ttcaggccac
     1381 cggcgttctc aacgtgggta tatcccatct cgctaaggag ctcttttgcc tgcccggact
     1441 ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg gcggtggcaa
     1501 tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc tcttgctgat
     1561 actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc agtgaaaaca
     1621 ccagtgccag agcaagtaag ccttttttta aacatgtaag cgtccttcta tggatgaaaa
     1681 ttgccatcca ttatacgcct gagatttaac aatttttgcg aaactcccct caccttttat
     1741 aacgctgtgc cagtttatta gcagcccgac ttaacagcgg ttccagcgcc acagccagca
     1801 acattttcag cggtcggcga gcaactgatt ttatcgccca gcccgccaca cccgccgggc
     1861 catagcgcag tgcggtaaga agtaccagct tgcctgctaa tttgaaacca agcttcacct
     1921 tttgcccggc ctgttgccag cgagtattca taactttcct cacagttgac ggaaacggct
     1981 acgtaacgtg aaagtatcgg aagtgacata acgttccatc tcgcgtaaac gcgtttcact
     2041 tgccgccagt tcacggtcga cttcatccag caattcgctg ctggaaggta gctgctcacc
     2101 aaaggccatg ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag
     2161 ggtaaacagc gccagaccga agaaaatcga cagcaccacc aggatacgca ccagttttac
     2221 cggtacatca aaatagttgg caatcccggc gcagacgccg cggaccatgc cctgctgtgg
     2281 aatacgccat aactttttat tgagattaat gcccgccatt agcgatccct ccagttcgga
     2341 tgttctgcat caagaataga ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca
     2401 tcaaccagtt gcgctaatcg ctgctgctca ctttgcgaca attcactgcg accagaacga
     2461 ttgctgtaat gcagccataa ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga
     2521 atagccagaa atagcgcgct cataagtact ccttagacga tgagcggcgg cgcgttcgga
     2581 cgccgcctgg atgttattat tgattgtctt gcttcatttt ggcttttaat tgtgccagtt
     2641 gttcgctgat tgcatcatcg gctttcagtt cggcaaactg atcgtccagc gatttttgtt
     2701 taccgaagct gtggctttct gcttccgctt ccatctggtc aatacgacgt tcgaaagatt
     2761 cgaaacgagc cattgcttca tccagtttgc cactgtccag ctgacgacgc acatcgcgcg
     2821 acgagtttgc cgcctgatga cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca
     2881 atttgttttc cagctcgcca atctctttct tcatgcgtgc cagcgtatcg tccaccagcg
     2941 tcacttcatg ttccagggac ttaatcagat cggtcagttt ctgtttttca attaacgctg
     3001 cacgtgccag atcctctctc tctttcagca gcgccagttc ggctttttcc tgccattcaa
     3061 cttcacgcgc cgacgcttgt tcaatacggc gagtcagctg tttcttttct gccaacgcac
     3121 gcgccgaagt agaacgtact tcaaccagtg tatcttccat ctcctggatc atcagacgca
     3181 ccagtttctg tggatcttcc gctttctcta acagagcgtt gatgttggca ttcacgatgt
     3241 cggcaaagcg agaaaaaata cccataatgt tgtcctcttg atttctgcgt tcaggattgt
     3301 cctgctgaac tgttaataca atttgcgtgc caatttttta tctttttgat ttataaagat
     3361 ctgattgaag aatcaacagc aacatgccag gatgaattag cgaattacac taacaagtgg
     3421 cgaatttcat catggccgaa tacaaagata atttacttgg tgaggcgaac agctttctcg
     3481 aagtgctgga acaggtttcg catctcgcac cgctggacaa accggtgctc atcatcggcg
     3541 aacgcggcac cggtaaagag ttgattgcca gccgcctgca ttatctctcc tcccgttggc
     3601 aagggccgtt tatttccctt aactgcgcgg cgttaaatga aaatctgctg gattccgaac
     3661 tgtttggtca cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcct gggagatttg
     3721 aacgtgccga cggcggtacg ctatttcttg atgaactcgc tacggcaccg atgatggtgc
     3781 aggaaaaatt attgcgtgtg attgagtacg gtgaactgga gcgcgttggc ggtagccagc
     3841 cgttgcaggt gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca
     3901 atgaaggtac ttttcgcgct gacctgctcg accggctggc tttcgatgta gtgcaactgc
     3961 cgcccctgcg cgagcgcgaa agcgacatta tgctgatggc agaacacttt gccattcaga
     4021 tgtgtcggga aatcaagctg cctctgttcc cggggtttac ggagcgcgcc agagaaacat
     4081 tgctgaatta tcgttggccg ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag
     4141 tgtatcgcca cggcaccagc gattatccgc ttgatgacat cattattgat ccctttaaac
     4201 ggcgtccacc tgaagaagcc atcgccgttt cagaaaatac ctcgcttccg acgctgccgc
     4261 tggatttacg cgagtttcag atgcagcagg aaaaagagtt gctgcaactc agtttgcaac
     4321 aggggaaata taaccagaag cgcgcggctg aattactggg gttaacttat catcagttcc
     4381 gcgcgttgtt gaaaaagcac cagatttagc gaagttgagc aatacaactt gccggatgcg
     4441 gcgtgaatgc tttattcggc atacataatc aatacgtcgt ttgcaggcct gataagcgca
     4501 gcgcatcaga cattattgcg ttgggctatt aatcgctcag cgcatcctgc aaaattttca
     4561 ttgccgcatc gaattgcgca tccgggatgg tcagcggata caggaagcga atcacgttgc
     4621 cgtatgcgcc acaggtcagc aggagcagcc cctgcgccag cgcgcgttgc tggattttct
     4681 gtgcaatcgc cgctgacggc tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg
     4741 accccaggcc gcgtaccgca gcaatggccg gaacgctttc tttggcatca atcaacgtgt
     4801 ttttgagacg ctggcccagt tgattcgcgc gttcgcagag tgattctttg tcgataatat
     4861 tgagcaccgc gtgcgcggca gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc
     4921 cgcccggcgc gggcgcgtcc ataatattcg cgttaccgac cacgcccgaa agcggcatcc
     4981 cgcccgcgag gcttttcgcc atcgtcatta agtccggctt atcggcgtaa tgatccatgg
     5041 caaacagttt accggtacgc gcaaagccgc tttgcacttc atcggcaatc atcacaatac
     5101 cgtgctcgtc gcacaggcgg cgaatggcgg caaccagctc ttttggcgca acgttgaaac
     5161 cgcactcgcc ctgcaccggt tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg
     5221 atttaaacaa gcgttcgatg gcgtcgaggg agtcctgtgt tgaaatgccg tgtaaatctg
     5281 acggataagg tacgtgatac accgaaccag ggaacgggcc gaagccgatt ttgtacggcg
     5341 caacttttcc ggtcagcgcc atggtcatat atgtacgacc gtggaagcca ccgctaaacg
     5401 caatcacgcc aggtcgtccg gtatgggcgc gagcaatttt caccgcgttt tccaccgctt
     5461 ccgcgccggt agtgaagaat gcggttttag cctgcccgct cactggggca agggtgttaa
     5521 ttttctccgc cagcgtgacg tagctttcgt acggcacaat ctgatacgcc gtgtgggtaa
     5581 actgttgcaa ttgctgctcc accgccgcga ccagatcagg gtggcgatgc ccggtattca
     5641 gcaccgcaat gcctgcggcg aaatcgatgt attcgttgcc ctctacgtcc ttcagcgtag
     5701 cgttttctgc cgactgggcg aagaagttac acatcacgcc aaccccgcgt ggggtagcag
     5761 aaagacgacg ctgatggaat tcattgttgc tcatgtttca caccttttga ctcttcactg
     5821 ttaatcggaa attaaacgcc cagacgatcg cgcaggctgt aataagccgc gcccatcgcg
     5881 gtaaatggca cgcgcagcgt gcgcccgccg gggaacgggt aatgcggcag attggcgaag
     5941 gcatcgaaac gctcggcgtc accgcgcagc agttcagcaa tcaaacgccc ggcaagatgg
     6001 gtacaggtga cgccgtggcc gctgtaaccc tgcatgtaat agatgttggt atcaaggcgg
     6061 ccgaactgcg gcatgtgcga caaggtcagc aggaagttgc ccgtccagcg gtaatcaatt
     6121 ttcacgccct tcaactgagg gaaggttttt agtagtttcg gtaccacaag acgctcaacg
     6181 tcatccgggt tgcgtgcacc atacactaca cccccgccgt acagcaggcg gttgtcggcg
     6241 gtaagacggt agtaatcaag cagatagtta cagtcttcca cacagtagtt tttcgggatc
     6301 agcgaacggg ctaaatcttc cgacagccgt tcggtggtga tcacctgcgt gccgcacggc
     6361 atgctgcgtt tcgctagttc cggctctact ttatcgccca gatacgcatt cccggcgaca
     6421 atcacatact tcgccgtcac ctgaccattg gcagtccgaa ccacggctgg cgtggtgtgc
     6481 tggatctgcg tcacggcgga aagctcatac acgcgcccgc cgttcaggcg gatggcatcg
     6541 gcttcaccaa tggcgaggtt tagcgggtga atatgcccac cgctgtgatc cagcagcgca
     6601 ccagtgtagc gatcgctggc gacctcgcgg cgaatagcgt tgacgtccag cagctccagc
     6661 tgtttattgc cgtagcgttc ccagttctct ttctgctctt cgagtgtggc gagttgttta
     6721 tcattcatcg ccacaaacag accgccgggg cggtagtcgc agtcaatctg ataacgtttg
     6781 atacgttcgc ggatgatctc gccaccctcg aacatcatgc tgccgagcat acgggcggtg
     6841 tccatgccgt agcttttttc gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc
     6901 ccgccattgc gcccgcttgc gccaaagccg atgcgtgagg cttcgagaac cactacgtca
     6961 aagcccgctt ccgccagatg cagcgcggag gagagcccgg tatagccgcc gccaaccacg
     7021 caaacgtcgc aggtgattga ctcattcagt gtgtcgaatg gtgcatattt attcgcactg
     7081 gcggcgtagt aactgctggt atgttcggtc atttcaggcc tccaggctta tccagatggt
     7141 tttcagttca gtgaattttt caagggcatg cagggatttg tcgcgaccgt tgccgctctg
     7201 cttatagccg ccaaacggca cggtcatatc gccgtcgttg tagttattga cgaagacgga
     7261 accggctttc aggcgtcggc tcatgcggtg cgcgcgggag aggtcgcgcg tccataccgc
     7321 cgcgccaagg ccgtactggc tgtcgttggc aagctgcagc gcctgatctt ctgatgtgaa
     7381 acgggtgacc accagcaccg gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc
     7441 cacatccacg aagatggtcg ggccgatggc cgctgccagc ccggcgttgc gaccatccag
     7501 cagcagttgc cccttgcctt cgccttcacg aataaaggtg tgtaccgagt cagcgtgagc
     7561 gctgtcgatt aaggtgccca tggtggttgc gggatcaagt ggatggcccg gctgccagtt
     7621 ttgcgcctgc tgttttaaca gggctaagaa ttcatcggcg atgctctctt ccagtaacaa
     7681 acgcgttccg gcgatgcaca cctggccctg gttgtagaag atgcctgcgg cggtggcgct
     7741 tgccgctttt tgcagatccg gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc
     7801 ttccagccag acacgtttca tgttgctatc acccgcgtct ttcagcagtt gtttacccgt
     7861 acgcgtggag ccggtaaagg cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc
     7921 cgcttcatga ccaaaacccg tcaccacgtt caacacacca tccggcaagc ctgcttcttt
     7981 cgccagcccc gcgagacgaa tcgcactgag cggtgatttt tcagacggtt ttagaatcac
     8041 gctgtttccc gccgccagcg ccgggccgag tttccagcaa gtcagcaaca gcgggaagtt
     8101 ccacggcacg atggcggcaa tcacgccgac cggttcacgc acgatcatcg ccagctcatg
     8161 gctactggtg gtcgccactt cgccatacac tttgtcgatc gcttcggcgt accagcgaat
     8221 ggcgcgcgcc gcgccgggaa tatcatcacg cagactgtga cgaatcggtt tgccggtgtc
     8281 gagagtttcc agcagtgcca gctcttcggc gtgggcttcc attaaatcgg cgagtttatt
     8341 cagtaccgct ttacgtttag ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc
     8401 gcgtgctgcg ctcaccgcac ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag
     8461 cggtgcctgg gtgaccggat caacggtttc aaaggtttca ttttccgccg cagcagtata
     8521 ttcaccgtta ataaataagc ggttttcaat ggcgagactt aacgctttat cctgccagta
     8581 agccagatga tgaaaattca ttatgactcc tgtttcacgt ctatcagata tatgcagttt
     8641 gtgaacgtca gggcgcagag atccccgggc tgcatgcccg tttatttctg gcgacattct
     8701 ttatggattg ccgggcgcat tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg
     8761 gtgggcgtat gggcgctgat aattcggcaa atacctgccg acgtattact gaaactgtgc
     8821 gggatgccgg tattaatggc ataactttgc cccgcgacga ggtggtaatc ctgaccatta
     8881 atcgtcagaa caatttcacc ttccagtact gtgcctattt cctcaccctg atgcttaatt
     8941 ctttccccag tggttgtgcc cggctggtac gtttcaaaga tcatcgccag cgtgcgattc
     9001 gggttaccgt tatgaaccag cttcattgac acaccctgac tgcccatctc aattaagtcg
     9061 tcctgattaa tgacgacctg cggctcatca ggtttttccg gctcggaaaa gaattccgag
     9121 agtgacaaac catacacctt cagcagcttt tgcagcgtac tgatggcagg gctgacttta
     9181 tcttgttcta tcgtactgat agcactgtga gtcagcccgg agagttcggc ggcacgacgt
     9241 tgtgaaagcc cctgctgctg gcggatttcc gacaagcgtt ttcctggcgc cagtccctca
     9301 tcactcatat ttgcatttcc ttaaactgta gtggtcagag tcgctgtttt tcagcgatat
     9361 ggtgctgcca agcggtgata aagccctcga acaatatacg cgaaagcgcg tactcgctac
     9421 tgttccattc cgggtgccac tgtacgccca gcgcaaaagg atgattgatg acgctaaccg
     9481 cctcaactaa gccatccggc gagcgagctt caacacgcaa ccgtgggcta acgaccttcg
     9541 ccccttgtcc atgtagagag tttacccaaa agttgctaca ttcaggtaac aacgcagaca
     9601 gtaatccccc ctcttcaacc tgaacttcat gcgacggtgc atattgctgt tccaccggca
     9661 gttcgggatc ttcccgatgt tccagcaatt caggctgttc gcacagcttg cgatgcaacg
     9721 aaccaccggt tgccaccacc agttcttgta aaccccggca gatggcgaaa atggggatgc
     9781 gcctttcgag tgcggcatta attatcgcca tgctcagaag atcacgcccg ggatcggcgt
     9841 caggctcatc gccgttttca ccatataggt gcggctgcac attgctggga ctactaggaa
     9901 gataaatgcc atcgagtttc ggcaaaagtt gttcaagtaa cgacggttcc gccagcgcat
     9961 gtggtagcgc aattggcaag ccgcctgcgt ggatgatggc attcaggtac ttttcttgca
    10021 gagtctgggt cgcatgaccc ttaagcctgt tcctgcacat tacgacaccg ataaccggat
    10081 tgttcattat attttccatg ctcaatctca caaagtggac taaattatcg ccattactgc
    10141 gctttaaatg acctttatgt tcaatatttt ttcaatctag cagtggattt tcatttttgc
    10201 aaactcaatt taacatttga caaacatttg gtttgcatac agattcgaat gatggtcatt
    10261 atattttacg ctttgataac gagcggaaaa caaaccaaag gcgaagaatc atggaaacca
    10321 atatcgttga agtagagaac tttgttcagc agtcagaaga gaggcggggt agcaccttta
    10381 cgcaggaagt gaaacgctac ctggagcgct acccgaatac gcaatatgtt gatgttttac
    10441 ttactgattt gaatggttgc ttccgtggaa aacgtatccc tgtttcaagc ctgaagaagc
    10501 tcgagaaagg gtgttatttc ccggcctcgg tatttgcaat ggatattctg ggcaacgtag
    10561 tagaagaagc aggtctgggt caggaaatgg gcgagccgga tcgtacctgt gttcctgttc
    10621 tcggttcctt aactccttct gccgccgatc cagagtttat cggtcagatg ctcctgacca
    10681 tggtcgatga agatggcgct ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt
    10741 ggcagcagct gcgccagcgc ggattgttcc cggttgtagc ggtagagctg gagttctatt
    10801 tactggatcg ccagcgcgac gctgaagggg atctgcaacc gccctgcgcg cctggcaccg
    10861 atgaccgcaa tacacaaagc caggtttact ccgttgataa cctcaaccac ttcgccgacg
    10921 tgctcaatga tattgatgaa ctggcgcagt tgcagctgat tccggcagat ggcgcggtcg
    10981 ctgaggcctc gccgggtcag tttgaaatca acctttacca tactgataac gtgctggaag
    11041 cctgcgatga tgcgctggca ctaaaacgcc tagtgcgtct gatggcagaa aagcataaga
    11101 tgcacgccac ttttatggcg aagccgtatg aagagcacgc gggcagcggg atgcatatcc
    11161 atatcagtat gcaaaataat cgtggcgaga atgtgctttc tgacacagaa ggcgaagatt
    11221 cgccgctgct gaaaaagatg ctcgccggga tgattgacct gatgccgtcg tcgatggcgt
    11281 tgctggcacc aaacgtgaac tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc
    11341 aggcgtcgtg gggccataac aaccgcaccg tcgccctgcg tattccgtgc ggtgaccgtc
    11401 ataatcaccg cgtggaatat cacgtggcgg gtgccgatgc caacccatat ctggtgatgg
    11461 cagcgatttt tgccggtatt ttgcatggcc ttgataacga gctgccgttg caggaagaag
    11521 tcgaaggcaa cgggctggaa caggaaggct tacccttccc gattcgccag agcgatgccc
    11581 tgggtgagtt tatcgagaat gatcacctgc gccgctattt aggcgaacgg ttttgccatg
    11641 tgtatcacgc ctgtaaaaat gatgagctgt tgcagtttga gcgcctcatc acagaaacag
    11701 aaattgagtg gatgttgaaa aacgcgtaat tccccttgcg gggttgtttc atcggcctga
    11761 gcggagccag gcctgacatt tgctggtaat ccagacattt cccggatgtc gcgttatgga
    11821 gcgcaggcgg caacgggctt ccgttaacga ttaagactgt gacgaggaaa tgagatgatg
    11881 ccgatgatta aatccccgca tggcgaggga ggctgcgtat gcgcccctcc tgctactgac
    11941 tggactccgc cccctttgct tcccttgcta aacaggtttg atttcaggtc gacacgaccg
    12001 caaaccttat tacgcagggg aggcagcaat tatggctatt aattcaccac tgaatattgc
    12061 tgcgcaaccc ggcaaaaccc gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat
    12121 gggtctggcc tatctcacgc cgatgaccgt atttgatact tttggtattg tctccggcat
    12181 tagcgacggt cacgttccgg cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc
    12241 tatcagctac ggcaaactgg ttcgccagtt tccggaggcc ggttcggcct atacctacgc
    12301 gcaaaagtcg attaacccgc acgtcggatt tatggtcggc tggtcatcac tgctggatta
    12361 tctctttttg ccaatgatca acgttttgtt ggcgaaaatc tatctctccg ccctcttccc
    12421 ggaagtgccg ccgtgggtgt gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct
    12481 gaagagcgtc aacctggtcg ctaacttcaa taccctgttt gtactggtgc aaatctccat
    12541 catggtggtg tttatcttcc tggtggttca gggactgcat aaaggagaag gcgttggcac
    12601 cgtctggtca cttcagccgt ttatcagcga gaacgcgcac ctgatcccga ttattaccgg
    12661 tgcgacgatt gtctgtttct cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga
    12721 gacgccggac gccgcacgcg tgatcccgaa agccatcttc ctgacggcgg tatacggcgg
    12781 cgttatcttt atcgcggcgt cgttctttat gcagctgttc tttcccgata tcagccgctt
    12841 taaagacccg gacgccgcac tgcctgaaat tgcgctgtac gtcggcggca agctctttca
    12901 gtcgattttc ctctgcacca cgtttgtgaa cacattagcg tctggcctgg cttcacatgc
    12961 cagcgtgtcg cgtctgctgt atgtgatggg gcgtgacaat gtgtttccgg agcgcgtgtt
    13021 tggctatgtg cacccaaaat ggcggactcc ggcactcaac gtcattatgg tcgggattgt
    13081 cgcgctgtcg gcgctgttct ttgatttagt caccgcgaca gcactgatta acttcggtgc
    13141 tctggtggcg tttaccttcg tgaatctgtc ggtgtttaat catttctggc ggcgtaaagg
    13201 aatgaataaa agctggaagg atcacttcca ctatttgctg atgccgctgg ttggcgcgct
    13261 gacggtgggt gtgctgtggg ttaacctaga gtcaacgtca ctgacactcg gtctggtatg
    13321 ggcttcgtta ggcggcgcat atttgtggta tctgatccgc cgctatcgca aagtgccgct
    13381 gtacgaaggt gacagaacgc cggtgagcga aacgtaatag ccggatgcgc ggcgcattat
    13441 cctgcctgag ggtaacgcgc cgcgccatta actataaaaa gatgtatgac ccggatcacg
    13501 agtgctatca tccgccctta tgcttagcgc attaacccca ggggcaaata atgaaccacg
    13561 atatcccgct aaaatatttt gatattgccg atgagtacgc gaccgaatgc gcagagcctg
    13621 tcgcagaggc tgaacgcaca ccgctggccc actacttcca gttactgctc acccgtttga
    13681 tgaacaatga agagatcagc gaagaagccc aacatgaaat ggctgctgaa gcggggatta
    13741 atcccgtacg cattgatgag atcgcagagt ttttgaatca atggggtaat gagtaattat
    13801 ttaattgcag acgatcggtc agtatcgttg tccggataaa gcgttcacgc cgcatccggc
    13861 taacaatgac acgcactgca gcgtgcttta atgggtttct cacgatcgta ccgctctgct
    13921 cctcgctaag ttgttctgtg agttatagtt atagagaaat cacgggtacg aatgttgaac
    13981 agatggcgac gccacctgtt caacgaccaa ccacacggcc ctcattacga cccctgaaca
    14041 caaacggtaa tggcaaatgt cattaaatat tagtaagata gcctctttac ataaaatccc
    14101 ttaatattat gcgccaggta ttatcgtctc ttttggtgat tgctggactt gtgagtggtc
    14161 aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat ccgcgacagc ggttttgtct
    14221 attgcgtcag cgggcaagtc aacaccttta acccatccaa agcgagcagt gggttaattg
    14281 tcgataccct tgccgcccag ttttatgatc gactgctgga tgtcgatccc tatacctatc
    14341 gcctgatgcc tgaacttgcc gaaagctggg aagtactcga caacggcgcg acctatcgct
    14401 tccacctgcg tcgcgatgtt ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa
    14461 tgaatgccga cgatgtggtg tttaccttcc agcgaatttt tgaccgcaac aacccgtggc
    14521 ataacgtcaa cggcagcaac ttcccctact tcgacagcct gcaatttgcc gataacgtga
    14581 aaagcgtccg caaactggat aatcataccg ttgagttccg actcgcccag ccggatgctt
    14641 cttttttgtg gcacctcgca acccattatg cttcggtcat gtcggcagaa tatgcccgga
    14701 agttagagaa agaagatcgc caggagcaac tcgaccgtca accggtcggc accggtccgt
    14761 atcagttgtc ggaataccgc gccgggcaat ttattcgcct acaacgtcat gatgacttct
    14821 ggcgcggtaa accgttaatg ccgcaggtag tggtggattt aggctccggc ggcaccggac
    14881 gtctgtcgaa actcctgacc ggggaatgcg acgttctggc ctggcctgct gccagccagc
    14941 tatccatttt gcgtgacgac ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg
    15001 cctatctggc atttaacacc gccaaaccgc cgctaaataa tcccgctgtc cgccatgcac
    15061 tggcactggc gattaataac cagcgcctga tgcaatccat ctattatggt acggctgaaa
    15121 cggcggcctc tattttaccg cgcgcctcgt gggcctatga caacgaggct aaaattactg
    15181 aatacaatcc ggcgaaatcg cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc
    15241 tgaaactgtg ggtgcccaca cgttcgcagg cgtggaaccc cagtccactg aaaactgccg
    15301 aactgattca ggcggatatg gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag
    15361 gtcgctttca ggaggcgcgg ttgatggata tgagccatga tctgacgtta tccggttggg
    15421 cgacggacag taacgacccg gacagtttct tccgtccgtt actgagctgc gcggcaattc
    15481 attcacagac caacctcgcc cactggtgcg atccgaaatt cgacagcgtg ttgcgtaagg
    15541 cgctctcctc gcagcagctg gcagcgcgta ttgaagccta tgacgaagcg cagagtattc
    15601 tggcgcagga attacctatt ctgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt
    15661 acgatatcaa aggtctggta cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc
    15721 gcgagaaaca ggatgaggtg aaaaaaccat gattatcttc accttacgcc gcattttgtt
    15781 attgattgtc accttgttcc tgctgacctt cgttggcttc agcttgagct atttcactcc
    15841 acacgcgccg ttacaaggcg cgtcactgtg gaatgcctgg gtgttctggt ttaatggcct
    15901 gatccactgg gattttggtg tttccagcat taacggtcaa ccaattgccg aacagttaaa
    15961 agagctcttc ccggcgacga tagaactgtg catcctcgcc ttcggttttg cgctgattgt
    16021 tggcatcccc gtggggatga ttgccgggat tacgcgccat aagtggcagg ataacttgat
    16081 caacgccatc gccttactgg gcttttcaat cccggtgttc tggctggcgc ttctgttgac
    16141 actgttttgt tcactcacgc taggttggtt gcccgtttca gggcgtttcg atctgctcta
    16201 cgaagtgaaa ccgattaccg gttttgcgtt gattgatgcc tggctttcgg actcaccatg
    16261 gcgggatgaa atgatcatga gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc
    16321 ggttgcgcca acaactgaag tgatccgcct gatgcgtatc agcactattg aagtgtatga
    16381 ccagaactat gtaaaagcgg cggcgacccg cggtttgtcg cgctttacta ttttgcgtcg
    16441 ccacgttctg cataacgcgt taccgccggt tattcctcgc ctgggcttac agttttccac
    16501 catgctgacg ctggcgatga tcaccgaaat ggtttttagc tggccgggtt tagggcgctg
    16561 gttaattaac gccatccgcc agcaggatta cgcagccatt tccgccggag tgatggtgtg
    16621 tggctcactg gtcattattg ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc
    16681 tctgaaacat aaggaatggt atgccttacg atagcgtata cagcgaaaag cgcccgccgg
    16741 gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt
    16801 acggctgcgc gggactggct gtactgtgta tttttggcgg ctggtttgcg ccttacggca
    16861 tcgaccagca atttctcggt tatcaattat tgccgccatc atggtcgcgc tatggcgaag
    16921 tttctttctt cctggggact gacgacctcg gacgtgatgt tttaagccgt ttactgagcg
    16981 gagctgcgcc aaccgtgggt ggcgcatttg tggtgacgct tgccgcgacg atctgtggcc
    17041 tggtactggg caccttcgcc ggggcgacac acggcctccg ctcagcggtg cttaaccata
    17101 ttctggatac cttactggcg atcccttcgc tgctgttggc aattatcgtt gtagcgtttg
    17161 ccggaccgag tttgtcccac gctatgtttg ctgtctggct ggcattgctg ccgcgtatgg
    17221 tacgttcgat ttacagcatg gtgcatgacg aactggaaaa agagtacgtt atcgccgccc
    17281 gtctggatgg cgcatcaacg ctgaatattc tctggtttgc tgtgatgcca aacatcaccg
    17341 ccgggctggt caccgagatc acccgcgcac tgtcgatggc aattctcgat atcgccgcgc
    17401 tgggctttct cgatctcggc gcacaactcc cctcgcctga atggggagcg atgctcggtg
    17461 atgcgctgga actgatttat gtcgccccgt ggactgtcat gctgccaggt gcggcaatta
    17521 tgattagcgt gttgttggtt aacctccttg gcgatggcgt tcgtcgtgcg attattgcgg
    17581 gggtggaata atgccattac tcgatattcg taacctgacc attgaattta aaaccggtga
    17641 tgagtgggtt aaagccgtcg accgggtaag tatgacctta accgaaggtg aaatccgcgg
    17701 tcttgttggt gaatccggtt ccggcaagag tttgattgcg aaagcgattt gtggggtgaa
    17761 taaagataac tggcgtgtta ctgctgaccg tatgcgtttt gatgatatcg atttgctgcg
    17821 tctctccgca cgcgaacggc gcaaactggt tggccataac gtgtcgatga ttttccagga
    17881 accgcagtcg tgtcttgacc cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc
    17941 agcctggacc tataaaggcc gttggtggca gcgttttggc tggcgcaaac gccgtgccat
    18001 tgaactgctg caccgcgtgg ggattaaaga tcacaaagat gcgatgcgca gttttcccta
    18061 tgagttgacc gaaggtgaat gtcagaaagt gatgatagcc attgcactgg cgaatcaacc
    18121 gcgtctgctg attgctgacg aaccgacaaa ctcaatggag ccaacaaccc aggcgcaaat
    18181 ctttcgcctg ctgacgcgtc tcaaccaaaa cagcaatacc actattttgc ttatcagcca
    18241 tgacttacaa atgcttagcc aatgggcgga taaaattaac gtgctttact gcggtcaaac
    18301 agtggaaacc gcgccgagta aggagttggt gacgatgcca catcatcctt atacccaggc
    18361 gctgatccgc gcgataccag acttcggcag cgcgatgccg cataaaagtc gcctcaatac
    18421 tctacccggc gctatcccat tgctggaaca gttaccgatt ggttgtcgtc tggggccacg
    18481 ttgcccgtat gcacaacgag aatgcattgt gacgccacgt ttgacggggg cgaaaaatca
    18541 tctctatgcc tgtcatttcc cgctgaacat ggagaaaaag tgagatgatc gaaacgctgc
    18601 ttgaagtgcg taatttaagt aaaacgttcc gctaccggac cggttggttt cgtcgtcaga
    18661 ccgtagaagc ggtaaaaccc ttgagcttta cgctacgtga aggccagaca ctggcgatta
    18721 ttggcgagaa tggttcgggt aaatccacac tggcaaaaat gctggcggga atgatagaac
    18781 ctaccagcgg cgagttattg attgacgatc atccgctgca ttttggcgat tattccttcc
    18841 gtagtcagcg tattcgcatg atttttcagg atccttcgac ctcgttgaat ccccgtcaac
    18901 gtatctcaca aattctggat tttccactgc gcctgaacac cgacctggaa ccggaacagc
    18961 gtcgcaaaca gattattgaa acgatgcgta tggttgggct gctaccggat cacgtcagtt
    19021 actatccgca tatgttggca cccggacaaa aacagcgtct gggtctggcg cgcgcgctga
    19081 tattgcgccc aaaagtcatt attgccgatg aagccctcgc ttcactggat atgtcaatgc
    19141 gttcgcagtt gattaatctg atgctggagt tacaggaaaa acagggcatt tcgtatattt
    19201 atgttaccca gcatatcgga atgatgaagc acattagcga ccaggtgctg gtgatgcatc
    19261 agggcgaggt tgtcgagcga ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac
    19321 tcaccaaacg gctgattgcc gggcactttg gcgaagcatt gacagcggat gcgtggcgta
    19381 aagatcgcta attttctgaa tcctccttcc agattattca cctcattctg acaagagatt
    19441 aaatcttgtc agaatgaggt gatggataaa ataaaatcaa atgcgcgtga tttaagacgc
    19501 aatctcactt tgcaggaacg aaagctctgg cgatatcttc gcagccgacg ttttggtgat
    19561 ttcaaatttc gccgtcaaca tccggtgggg agctacattc tcgattttgc ttgctgctcg
    19621 gcgcgcgtag tcgttgagct ggatggtggg cagcatgatt tggcagttgc ctatgatacc
    19681 aggcgcacta gctggcttga gtcgcagggc tggaccgtgc tgcgtttctg gaataacgag
    19741 attgattgta atgaggaggc ggtgctggag attattctgc aggaactgaa ccgccggtca
    19801 ccctcaccct gaaagggcga gggggccgac cgagccgaat agctgttgtg gtgaaaacat
    19861 ggagacagtg ctggagaata ttcggcaagg tctgaaccat cccagccatc gccataaaag
    19921 ggttaggggc tgtatgagcc tgtttgttgc tggggtaaca atatttgcac aatatggtcc
    19981 cctcgcccct ctggggagag ggttagggta aggggaaaag cgccccccct gccgcagcct
    20041 gctccggtcg gacctggcaa ctatagctac tcacagccag gttgattata ataaccgttt
    20101 atctgttcgt actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc
    20161 aacaataagg attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt
    20221 tgccagcaaa ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga
    20281 actggcattc acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca
    20341 attgggttct gacatcgttc tgcagtgcga tgttgcagaa gatgccagca tcgacaccat
    20401 gttcgctgaa ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt
    20461 tgcacctggc gatcagctgg atggtgacta tgttaacgcc gttacccgtg aaggcttcaa
    20521 aattgcccac gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat
    20581 gctgaatccg ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc
    20641 gaactacaac gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc
    20701 gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac
    20761 tctggcggcc tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac
    20821 cccgattcgc cgtacagtta ctattgaaga tgtgggtaac tctgcggcat tcctgtgctc
    20881 cgatctctct gccggtatct ccggtgaagt agtccacgtt gacggcggtt tcagcatcgc
    20941 tgcaatgaac gaactcgaac tgaaataatc gttctgttgg taaagatggg cggcgttctg
    21001 ccgcccgtta tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc
    21061 cttcccgcct tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa
    21121 cgccacatca ccggcaaaag ccactggtat catgaaacgc aatccagtac tacggagtat
    21181 gacgttctgc ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg
    21241 atccttgatg aagagacgct ggcccccttc ctttcatggc tggtccctgc gcgcgttctt
    21301 gcagtggagt tgttccctga ccagcttacc gtgacccgtt cacaaacctt caccgcttat
    21361 gaacgcttgt cgacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac
    21421 tactattcgg cgcgacttac gccgctcccc ggacctgatt cctccaggga aagtaatcat
    21481 cggttggcac aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac
    21541 aaccgatcgc gccagcatct gaatgacgtc ggtcttactg tctgggactg tgtgatcatt
    21601 aaccaaatca ttggttttat tggctttcag gcgcggacaa ttgcgacatt tcaggcttat
    21661 ctcgggcatc cggtacgctg gttacccggg ctggcgatac aaaactacgc cgacgcgtca
    21721 ctgtttgctg atgaatcaat acgctggcga agcagctatg aggtggaaaa actacctgaa
    21781 gagcacacaa aaagttcaac tgcagaactt tgccaactgg ccgaaacaca ctctctccac
    21841 cctatttcac tttcccttct cgaaaagttg ttaaacagca cacgggtcaa tgcacagccg
    21901 gataatcagc ttgcggcgtt gttatgcgcg cgtataaatg gcagtcctgc ttgtttttcc
    21961 acctgtatgg attcatcaaa tgaatataaa aaaatcagca cccttctgcg caagggcgaa
    22021 aatgaaatta accgatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata
    22081 caatggctga cccgagcacc cgatcgcttt agcgccgccc agttcagccc tttactcgaa
    22141 cacgaaaaat catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg
    22201 ataaatcgct taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt
    22261 cgcgtaaaac tgtcagccgc tctaatggcc accaaaatag acaattatgt ttcaggacaa
    22321 cccgctgcta gcgcagctta aacagcaact gcattcccag acgccacgcg ctgaaggggt
    22381 ggtaaaagcc acagaaaaag gctttggctt cctggaagtc gacgcacaaa aaagttattt
    22441 cattccgccg ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggtgatcca
    22501 cagtgaaaaa gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg
    22561 tttcgtgggt aaagttcagg gcaaaaatga ccgtctgacc atcgttcctg atcatccact
    22621 tttaaaagac gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg
    22681 cgactgggcg gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc
    22741 tgaactgaca caatacatca cttttggtga cgaccacttt gtaccgtggt gggttaccct
    22801 tgcgcgccat aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga
    22861 aggtctggtt cgtgaagatc tgacctcgct ggattttgtc accatcgaca gtgccagcac
    22921 tgaagatatg gatgacgccc ttttcgctaa ggcgctgccg gatgacaaac ttcagctgat
    22981 tgtggcgatt gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc
    23041 gaaaattcgc gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga
    23101 gctttctgac gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg
    23161 catgacgctc tccgctgatg gcaccactga agataatatc gaattctttg ccgccaccat
    23221 cgaatccaaa gcgaagctgg tgtatgacca ggtttctgac tggctggaaa ataccggtga
    23281 ctggaagcct gaaagtgaag caattgccga acaagtccgt ttgctagcgc aaatttgcca
    23341 acgccgcggc gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg
    23401 ctttattctc ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc
    23461 caaccgtatc gtcgaagaag cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg
    23521 cgataagctc ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga
    23581 cgcgctggca gcgttgctga aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac
    23641 gctggacggt ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga
    23701 cagccgcatt cgtcgcttcc agtcatttgc tgaaattagc tctgaacccg gtcctcactt
    23761 tggcctcggt ctggaagcat acgccacctg gacttcgccg atccgtaaat atggcgacat
    23821 gatcaaccac cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccgcagga
    23881 tgaaatcact gtccaaatgg ccgaacgtcg ccgtctcaac cggatggcag aacgtgatgt
    23941 tggcgactgg ttatacgcac gcttcctgaa agataaagcc gggaccggca cccgtttcgc
    24001 ggcggaaatt gtcgatatca gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc
    24061 tatcgccttt attccggcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca
    24121 ggaaaacggc actgtacaaa ttaaaggtga aacggtttac aaagtaactg acgttattga
    24181 cgtcaccatt gccgaagtcc gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta
    24241 atctcctttc acggcccatt cctcatggat gggccgttta tttccccgct ctaccttcgt
    24301 tatatccagg cagatttttt aactgacttt cgtttgaaaa ctggcgtttt tccaaaactg
    24361 gattacaaat aaatatataa aaaacaatgc gtttagccgc cgttaatttc atgtaagtca
    24421 tgttgttcct tttatttaac cgcggactcc gctgttaacc ggaggatatg catcatgaaa
    24481 accgttaggg agtccacaac gttgtacaac tttctcggat cgcacaatcc atactggcgg
    24541 ttgacggaaa gcagcgatgc tttgcgcttt tctaccacca aaaccacaga acctgatcgt
    24601 acattgcagt tatctgccga acaggctgct cgcatcaggg aaatgacggt catcacctcc
    24661 agcctgatga tgagtctgac cgtcgatgaa agcgatcttt ctgtgcatct ggtaggacga
    24721 aaaatcaata aacgggaatg ggctggcaac gcgtctgcat ggcatgacac accggcggtt
    24781 gctcgtgatt tatcacacgg gctttccttt gctgagcagg tagtttctga agcacattcc
    24841 gcaatagtga ttctcgacag ccgggggaat atccaacgct tcaatcggtt atgtgaagat
    24901 tacacagggt tgaaagaaca cgacgtcatt gggtaaagcg tgtttaaact gtttatgagc
    24961 cgtcgtgaag ctgcggcatc caggcgcaat aaccgtgtat tttttcgaag cggcaatgca
    25021 tatgaagtcg aactgtggat accaacacgt aaaggccagc ggctgtttct gtttcgcaat
    25081 aaatttgtcc acagcggcag tggcaaaaac gagatttttt taatctgttc cggtaccgac
    25141 attaccgaag agcgccgcac tcaggagcga ctgcgtattc tggcaaatac cgacagtatc
    25201 accggactgc cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat taatcaggca
    25261 gataacaata aagttggggt tgtgtatctt gatttggata attttaaaaa ggtcaacgac
    25321 gcctatgggc atttgtttgg tgaccagtta ttacgcgacg tgtcattggc tattttaagc
    25381 tgtctcgaac atgaccaggt gttggcgcgt ccaggtggcg atgagtttct ggtactggca
    25441 tccaatacct cacaaagcgc gctggaagca atggcatcac gaattttgac ccgcttacgc
    25501 ctcccctttc gtattggttt aattgaagtt tataccagct gttcagtagg tatttcgctc
    25561 tctcccgaac atggttcgga cagcgcgact attattcgtc acgccgacac agcaatgtac
    25621 acagcgaagg aaggcggacg aggacaattt tgcgttttta ctccagaaat gaatcagcgg
    25681 gtatttgaat atctctggct ggataccaac ttgcgtaaag cactggaaaa cgatcagttg
    25741 gttatcgaaa atcacctagc gtggcgaagt gcgcagtctg gaagcactag tacgttggca
    25801 gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgccg aagagtcagg
    25861 gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcaaagtg
    25921 gcgggataaa ggcataaacc tgcgagtggc ggtaaatatt tctgcacgtc agctcgccga
    25981 tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc
    26041 tatagatgtt gaactgacag agagttgtct gattgagaat gatgaactgg cactgtctgt
    26101 tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccggcta
    26161 ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt
    26221 tgttcgagat attcacaaac aacctgtctc gcagtcactg gtccgggcga tcgtcgctgt
    26281 ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc
    26341 ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc
    26401 cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata
    26461 tatatactta gcgctatatc ccgtcattat atatcgtatt cctggatgat aattagccgt
    26521 tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc ccccttttgc
    26581 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    26641 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
    26701 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga agacaccgtg
    26761 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    26821 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    26881 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    26941 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    27001 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
    27061 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    27121 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag
    27181 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    27241 gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    27301 ggccctttga cacgccagtg catccagcag gtacatttca gcaaagcatt tattggtatt
    27361 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgcac cgatgtggtc
    27421 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    27481 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    27541 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
    27601 gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta
    27661 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacactct caagatataa
    27721 aattattatc agcgatataa caggaagtaa ttatcacctg cgtgatataa ccctgcgcgc
    27781 gagcagattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    27841 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    27901 gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt ctaactggtc
    27961 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    28021 gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg ttattggtca
    28081 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    28141 gattctgttc agtacgaaat aactgaataa tacacacagt aaaatattcg aaagccacgg
    28201 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    28261 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    28321 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    28381 atcgagatcg ataccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    28441 ctgaatacgc accgctccat cgcctttagg gcgtactggg gccgctttgg gctcatcaat
    28501 acgcccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    28561 tgtaaagagg cgttgatcgc tttcagtgtc tgcgctggat ctaccgattg cgttaccggg
    28621 cgaccaatca ccatataatc aacaccagcc gccaacgcct gttctggcgt cataatgcgg
    28681 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gtgtaaccag tttgaactcc
    28741 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    28801 tcacattttt gcgtcagtgc tgccagacgt tctgcataat ctgcaggtga cagtgtcacg
    28861 ccaagatcgg ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    28921 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    28981 ccggaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    29041 gctgcagtgt tggggatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    29101 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttacc gaccttcaga
    29161 cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcaca attatgataa
    29221 tcaagggcaa caaccacagg agaattcgta acggcgcggg aagaagatga agcagttaac
    29281 gtcatgacca gaccttcttg atgatgggca ccgggagtgg cgcgaaacag gtaaacggcg
    29341 agcattctac ccgtgaaaat aaaaaattaa cagttgcgat ttttccccta gcaggctttt
    29401 tctgctccgt cgttgccgga gcacaatggg acatcaataa ctagtatgtt gtaactaaag
    29461 tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat agttgaccag
    29521 gcacgacaag acggacaatg ccagtagagg gtgtatgcag taaaaccaca tttctggcag
    29581 ctataacgag gcttactacg caccttctcg ccaaccatgt cacgcagcac catcagactc
    29641 tctttggcgc gcccttcttc cgcttcattt aagtggtaat ccattagctt atggaataca
    29701 cgcatggtcg gatgacgctg aagctggcgt gtaatataga cctgtgcagc ctcactaccg
    29761 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    29821 accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    29881 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatgac gcgttgcaaa
    29941 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    30001 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    30061 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    30121 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    30181 tcactggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    30241 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    30301 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagcga ggcgctttcc
    30361 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    30421 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    30481 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    30541 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    30601 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    30661 ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    30721 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg
    30781 ccacaaatca accaaccgat agcaaacccc gcagcaaaca ataccgccag caatgtggaa
    30841 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    30901 cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    30961 acattacttc ccgttatgtg gttagagcga ataaattatg gtcaacttac cgcaatagcc
    31021 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt
    31081 taaggcctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gtgcaatttc
    31141 gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc
    31201 caccgccacc agcgcccacg aaatcaacgt agctaccacc agatcgcgtg gccaatgcat
    31261 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accaacaaga tagcaatggt
    31321 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa
    31381 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    31441 ttgtggaata tttttctctt cagccaactg ttcttttact agatttccgc gttctgctcg
    31501 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccaacc agataacaaa
    31561 aggtcgtggt tcctgtactt tgtctttgat ccaggattta acgccttgtc caacaaggat
    31621 tgccgccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    31681 gagaaaccag ccgaacaaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    31741 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc
    31801 agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtcg
    31861 ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaatgcc
    31921 agttccaaga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    31981 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    32041 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    32101 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    32161 tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    32221 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc
    32281 cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    32341 gaataaaatc cgcgcttacg cactgcagga tcaaggttac gataccgtag aggctaacca
    32401 ccagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    32461 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    32521 cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    32581 cgaacattat ctcgatacca aagccgagaa aatgggccat ttgctgaaca aataaccctc
    32641 ttgcatcgtg taattcattt gcttgccgga agcaaaataa ccggcaagca aatacttgtt
    32701 acttcaacat attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact
    32761 ccgtcgcggt gtcgatacga caacggcagg gtacgacttc ctggctacca tccgcgcgcg
    32821 taagcgtcac cggaaccgtc gcgccaggtt gtagattttg cagatcgcca atatcaatct
    32881 tctcttcccc ggttagccct aacgttttac gcgtcacgcc ttgcggaaat tccagcggca
    32941 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    33001 cacgaattcc aagcagacga ggtccttttg ccgcccagtc acggcttgag cctgatccat
    33061 actctttccc ggcaatcacc gccagcggcg tttgctcctg cttatagcgc atcgcagcat
    33121 cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    33181 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    33241 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat
    33301 atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    33361 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccact ggtgctggtg
    33421 ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgagtcc tcctgccaac
    33481 cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt
    33541 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    33601 gtgccgatgg ccagatatct ttcagataaa ccggctcgcc tttgcgatca tggccgatag
    33661 gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggtg
    33721 gcgaggccag ccagttagtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    33781 cggacagcac cgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    33841 gcagcggccc agagttacca atacaggtgg tacaaccgta tcccacaagg ttaaacccca
    33901 gttcgtcgag atacggtgtc agtttcgctt ttgccagata atcagaaacg actttcgaac
    33961 ccggtgccaa cgacgctttg acccacggtt gccgcttgag gccaagagtt acggcttttt
    34021 tcgccagcaa gcctgcggcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta
    34081 tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat
    34141 cgactggcag gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaatg
    34201 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg ttttggccct gccaggctcg
    34261 cttcaacgtc attcatatcc agttctaaca tactggtaaa aatcggttca tcgcccgggt
    34321 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcgacctga tcttcgctgc
    34381 gcccgcttaa acgcatgtaa tcgagggtta ctgcatcgat tgggaagaag ccacaggtgg
    34441 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccaacggt agtgaatcca
    34501 gaccatcacc ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc agcatttggg
    34561 taacagtgag aaccaggtct gtggcggtaa taccttcacg taattttccg gtaagtttaa
    34621 agcccactac gtccgggata agcatggaaa ccggctgg
//
LOCUS       DAECNO010000036        34445 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.036, whole genome shotgun sequence.
ACCESSION   DAECNO010000036 DAECNO010000000
VERSION     DAECNO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.036"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..332)
                     /locus_tag="KKO08_001785"
     CDS             complement(<1..332)
                     /locus_tag="KKO08_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318059.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="KKO08_001786"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="KKO08_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBD6318060.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="KKO08_001787"
     CDS             complement(1693..2073)
                     /locus_tag="KKO08_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBD6318061.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="KKO08_001788"
     CDS             complement(2087..2503)
                     /locus_tag="KKO08_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318062.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="KKO08_001789"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="KKO08_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBD6318063.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="KKO08_001790"
     CDS             complement(3504..3971)
                     /locus_tag="KKO08_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBD6318064.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="KKO08_001791"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="KKO08_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBD6318065.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="KKO08_001792"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="KKO08_001792"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBD6318066.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="KKO08_001793"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="KKO08_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBD6318067.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="KKO08_001794"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="KKO08_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBD6318068.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="KKO08_001795"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="KKO08_001795"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBD6318069.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="KKO08_001796"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="KKO08_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBD6318070.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="KKO08_001797"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="KKO08_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBD6318071.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="KKO08_001798"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="KKO08_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBD6318072.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="KKO08_001799"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="KKO08_001799"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBD6318073.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="KKO08_001800"
     CDS             complement(14626..15441)
                     /locus_tag="KKO08_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBD6318074.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="KKO08_001801"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="KKO08_001801"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBD6318075.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="KKO08_001802"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="KKO08_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBD6318076.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="KKO08_001803"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="KKO08_001803"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBD6318077.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="KKO08_001804"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="KKO08_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBD6318078.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="KKO08_001805"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="KKO08_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBD6318079.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="KKO08_001806"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="KKO08_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBD6318080.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="KKO08_001807"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="KKO08_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBD6318081.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="KKO08_001808"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="KKO08_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBD6318082.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="KKO08_001809"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="KKO08_001809"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBD6318083.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="KKO08_001810"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="KKO08_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBD6318084.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="KKO08_001811"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="KKO08_001811"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBD6318085.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="KKO08_001812"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="KKO08_001812"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBD6318086.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="KKO08_001813"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="KKO08_001813"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBD6318087.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="KKO08_001814"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="KKO08_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBD6318088.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="KKO08_001815"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="KKO08_001815"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBD6318089.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="KKO08_001816"
     CDS             31575..32189
                     /locus_tag="KKO08_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBD6318090.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="KKO08_001817"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="KKO08_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBD6318091.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="KKO08_001818"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="KKO08_001818"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBD6318092.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8104 a         8701 c         9305 g         8335 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggta
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaaccc accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc aggccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 attttctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgctaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gtgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtga tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgaacaccc
    34441 tgcgc
//
LOCUS       DAECNO010000037        33871 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.037, whole genome shotgun sequence.
ACCESSION   DAECNO010000037 DAECNO010000000
VERSION     DAECNO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.037"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1183
                     /locus_tag="KKO08_001819"
     CDS             <1..1183
                     /locus_tag="KKO08_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HBD6318093.1"
                     /translation="RWDKQQSFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSV
                     GVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYSEQDGDQTSWQL
                     SHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLS
                     AFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSR
                     TLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYRE
                     VSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQ
                     FLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVA
                     P"
     gene            1180..2268
                     /locus_tag="KKO08_001820"
     CDS             1180..2268
                     /locus_tag="KKO08_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBD6318094.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(2356..3654)
                     /gene="exuT"
                     /locus_tag="KKO08_001821"
     CDS             complement(2356..3654)
                     /gene="exuT"
                     /locus_tag="KKO08_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBD6318095.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4138..5550
                     /gene="uxaC"
                     /locus_tag="KKO08_001822"
     CDS             4138..5550
                     /gene="uxaC"
                     /locus_tag="KKO08_001822"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBD6318096.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5565..7052
                     /gene="uxaA"
                     /locus_tag="KKO08_001823"
     CDS             5565..7052
                     /gene="uxaA"
                     /locus_tag="KKO08_001823"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBD6318097.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7135..7686
                     /locus_tag="KKO08_001824"
     CDS             7135..7686
                     /locus_tag="KKO08_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBD6318098.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(7691..8935)
                     /gene="sstT"
                     /locus_tag="KKO08_001825"
     CDS             complement(7691..8935)
                     /gene="sstT"
                     /locus_tag="KKO08_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBD6318099.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9531..10496)
                     /gene="alx"
                     /locus_tag="KKO08_001826"
     CDS             complement(9531..10496)
                     /gene="alx"
                     /locus_tag="KKO08_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBD6318100.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(10757..11764)
                     /locus_tag="KKO08_001827"
     CDS             complement(10757..11764)
                     /locus_tag="KKO08_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD6318101.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLTSAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVNYTCQSKT
                     V"
     gene            complement(11843..12535)
                     /locus_tag="KKO08_001828"
     CDS             complement(11843..12535)
                     /locus_tag="KKO08_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBD6318102.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(12608..13111)
                     /locus_tag="KKO08_001829"
     CDS             complement(12608..13111)
                     /locus_tag="KKO08_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBD6318103.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            13196..14332
                     /gene="rlmG"
                     /locus_tag="KKO08_001830"
     CDS             13196..14332
                     /gene="rlmG"
                     /locus_tag="KKO08_001830"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBD6318104.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14617..14931
                     /gene="higB"
                     /locus_tag="KKO08_001831"
     CDS             14617..14931
                     /gene="higB"
                     /locus_tag="KKO08_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBD6318105.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14928..15344
                     /gene="higA"
                     /locus_tag="KKO08_001832"
     CDS             14928..15344
                     /gene="higA"
                     /locus_tag="KKO08_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBD6318106.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15389..17407)
                     /gene="fadH"
                     /locus_tag="KKO08_001833"
     CDS             complement(15389..17407)
                     /gene="fadH"
                     /locus_tag="KKO08_001833"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBD6318107.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(17633..19984)
                     /gene="ygjK"
                     /locus_tag="KKO08_001834"
     CDS             complement(17633..19984)
                     /gene="ygjK"
                     /locus_tag="KKO08_001834"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBD6318108.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINRNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20001..20420)
                     /locus_tag="KKO08_001835"
     CDS             complement(20001..20420)
                     /locus_tag="KKO08_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318109.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(20398..20751)
                     /locus_tag="KKO08_001836"
     CDS             complement(20398..20751)
                     /locus_tag="KKO08_001836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318110.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(20752..21072)
                     /locus_tag="KKO08_001837"
     CDS             complement(20752..21072)
                     /locus_tag="KKO08_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318111.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(21206..22639)
                     /locus_tag="KKO08_001838"
     CDS             complement(21206..22639)
                     /locus_tag="KKO08_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBD6318112.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(22702..23151)
                     /locus_tag="KKO08_001839"
     CDS             complement(22702..23151)
                     /locus_tag="KKO08_001839"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBD6318113.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23148..26225)
                     /gene="ebgA"
                     /locus_tag="KKO08_001840"
     CDS             complement(23148..26225)
                     /gene="ebgA"
                     /locus_tag="KKO08_001840"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBD6318114.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(26408..27391)
                     /gene="ebgR"
                     /locus_tag="KKO08_001841"
     CDS             complement(26408..27391)
                     /gene="ebgR"
                     /locus_tag="KKO08_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBD6318115.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            27609..27940
                     /locus_tag="KKO08_001842"
                     /pseudo
     CDS             27609..27940
                     /locus_tag="KKO08_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(27982..29361)
                     /gene="ygjG"
                     /locus_tag="KKO08_001843"
     CDS             complement(27982..29361)
                     /gene="ygjG"
                     /locus_tag="KKO08_001843"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBD6318116.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            29779..31299
                     /gene="aer"
                     /locus_tag="KKO08_001844"
                     /pseudo
     CDS             29779..31299
                     /gene="aer"
                     /locus_tag="KKO08_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(31453..32076)
                     /gene="nfeR"
                     /locus_tag="KKO08_001845"
                     /gene_synonym="yqjI"
     CDS             complement(31453..32076)
                     /gene="nfeR"
                     /locus_tag="KKO08_001845"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBD6318117.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            32353..33098
                     /gene="nfeF"
                     /locus_tag="KKO08_001846"
                     /pseudo
     CDS             32353..33098
                     /gene="nfeF"
                     /locus_tag="KKO08_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(33152..33227)
                     /locus_tag="KKO08_001847"
     tRNA            complement(33152..33227)
                     /locus_tag="KKO08_001847"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33192..33194),aa:Ile,seq:cat)
     gene            33352..33858
                     /gene="mug"
                     /locus_tag="KKO08_001848"
                     /pseudo
     CDS             33352..33858
                     /gene="mug"
                     /locus_tag="KKO08_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8247 a         8743 c         8917 g         7964 t
ORIGIN      
        1 gcgttgggat aaacagcaga gctttgaaaa taactatctt cgccagacaa cagcgcaaag
       61 tatctctgaa actgtacaac ttagcgcttc ccgcgctatt gtgatgcgtg actggatttt
      121 gagtacgtca gtcggtgttt tccatcgtaa tgacaacggt ggcgataacg acgacaacgg
      181 cttgtactta tcgttttcgt tatctgacac gccaacgatg gatagcaata acaacagcca
      241 ttcaaccaat gtgcctacgg attatcgtta tagcgaacag gatggcgatc aaacgtcatg
      301 gcagttatcg catacctttt ataacgattc attcagccat aaagaacttg gcgtaaccgt
      361 cggaggcctg aacaccgata ccataaacag cgcggttaac gggcgttggg atgggcaata
      421 cggaaatgtc tacgctaccg tatctgatag ttatgaccgt aagaatcatg atcatctttc
      481 ggcctttacg ggcacttata gctctacgct ggctgtcagt cgctatggcg ttaatttggg
      541 agccagtggt acagacgatt tgctgggtgc ggtactggtg gatgtgaaag gcttctctga
      601 acaggatgaa gagagtcagg atctgcaact cgaagcgcgg gtggcaggca gccgaacttt
      661 gcagcttggt caaagtgaca gtgtgttgtt cccttatcct ggatttcagt ctggttttgt
      721 tgaggttaac gacagtagcc agggcaatca gcaggggaca acaaacatca ttaacggtgc
      781 agggaatcgt gaattaatgt tgttgcctgg caagctgcgc tatcgcgaag tgtctgccag
      841 ctttaactac aactatatcg gtcgtttatt actgccggca gcggtgaaaa aattcccgat
      901 tgtggggctg aacagcgcca tgttactggt agctgaagat ggcggattta cacttgaaat
      961 taacggcagc gaaaaagagc tctatctgct ttccgggcag caattcctta agtgtccact
     1021 gagtgttgta aagaaacgcg ccagcattcg ttacagcgga gatgttactt gtagtgtggt
     1081 gacttattca caattaccgg agtcgattca ggttcaggca cagttgaaac agcctaaatt
     1141 acgtggaaac gttcagacgg cgcaaaggga ggttgcacca tgagaaatcg attgattgcg
     1201 gcgatattgg gcttgtttgg cacgctcact ggcgttcagg cagctcctga cgtgaccagt
     1261 gaaattacgt atgatttggc atctggtaga gcggattatt acttctggaa ggatgaagcg
     1321 tcggcaggaa ataatggata tatgtggtat gaatgttcgt atcctgacct ccaacaaacc
     1381 tgtacagcta atggaaatat atcgacagta caaatctatt taactgaaca acgcagtggg
     1441 atgcgttggc cggtaaaact caaaggattt aaaacagcca ttgtaagtag cgatgaagcg
     1501 ccgctaggat gcaagggggg caaagggctt cagacgaatc ttaaggattc taatagatct
     1561 tcatgtacag aagatggtca acattattat atatacgata caaagtttct tacgctctac
     1621 cttgagcaga cagagatgaa gaatttgccg attggtggcg tctggaaggg gaaagttaaa
     1681 ttacattcga acagcccggc ccaggactat ttcgcaaata ttaccctgaa tacgctcgac
     1741 cccaaccata ttgacgtgtt cttcccggag ttcgcccacg ccacgccaag ggtgcagtta
     1801 gacttgcatc caacaggaag cgttaacggc agcaactacg cgcaagatct gaccatgttg
     1861 gacatgtgcc tgtacgatgg ttttaacggt aatgccatca gttatgaaat catgctcaaa
     1921 gatgaagggc gaccagctgc agggcgcaga gacggttact tctctatcta tcgtcaggga
     1981 gggaccacca ccgacgaggg agaacgcatt gattaccggg tcaaaatgta caacccggaa
     2041 accggtgggc aaattgatgt gcgcaataat gaaaatatgg tctggaacag cattaacctg
     2101 aaacgtgtgc gtccggtggt actgcccggt attcgctatg ccgtgatgtg tgtgccgaca
     2161 cccttgactc tggcagtcga taaattcagc gtgatggata aacaggccgg gtactacatg
     2221 ggcaaattgt cagtaatctt tacgccttcc ttgccaacca tcaattaacc ctgcaaaccg
     2281 gacaaacgct tttttctata taaacgacga agccgcctga taaggcggct tcgtcgttat
     2341 aacttacgtt aaacgttaat gttgcggtgc gggatcgtta tgtgtttcct gcgccacctc
     2401 aatcgccggt ttgttctgca atacggtcca gataaccagc gcacccagca ggtcaaatac
     2461 tgccagaact gcgaacagcg ggctgaagcc gatggtgtca gccagtgcac cgacaaccag
     2521 cgcaaacagc gtacttgcca gccatgcgga catcccggtt aaaccgttcg ccgtcgccac
     2581 ttcgttacga ccgaacacgt cagaagagag cgtaatcagc gcgccagaca gtgcctggtg
     2641 ggcaaaacca ccgatacaca gcagcataat tgcgacatac gggttggtga acagaccgat
     2701 catacccggg ccaatcatca gcacagcacc cagcgttacg accatcttac gggaaacgat
     2761 caggttcaca ccaaaccaac gctggaacag cggcggcagg taaccaccga ggatacaacc
     2821 gaggtcagca aacagcatcg gcatccaggc gaacatcgcg atctctttca ggttaaagcc
     2881 gtaaacttta aacatgaaca gcgggatcca cgcgttaaaa gtaccccagg ccggttctgc
     2941 aaggaaacgc ggcagtgcga taccccagaa ctgacggtta cgcaggatct gaccaacgga
     3001 cattttcttc gccgtgctca cctggtgctg ggcttcctga ccattaataa tatagtcgcg
     3061 ttcttcatcg gtcagatgct tctggtcgcg cggatgttta tagaaaatca gccatgccat
     3121 cgcccagata aagctcaatg caccggagat gataaatgcc atctgccagc tgtgcatcac
     3181 gattgcccat accaccagcg gcggcgcaat catcgcacca atcgaagaac ctacgttaaa
     3241 gtagcctact gcgatggaac gctctttcgc cgggaaccat tcggagctgg ctttcagacc
     3301 cgccgggatc atcgctgctt ccgcggcacc gaccgcacca cgagcaacag ctaggccacc
     3361 ccagctacct gccagcgcgg ttgcaccaca gaacacggcc cacagcacag caaacattgc
     3421 ataaccgatt ttcgtaccca gcacatccag tacataacct gctaccggtt gcatgaccgt
     3481 ataagcagca gaataggctg cgatgatata ggaatactgt tgggtggaga tgtttaactc
     3541 ttccatcaga gttggcgcag ctgccgccac agtgttacgc gtcaggtaac caagcacggt
     3601 gccgagcgtc accagtgcga tcatatacca acgtaaccct ttaattttac gcatgtaaaa
     3661 cctcatcgtt ttgttatatc cgcaggaagc gcggccatca tcccgccaga ggctgaagat
     3721 gttagttacg accgggtaac cgaaaaaaaa accggcacgc cccgaacgtt gccatgaaag
     3781 tttcgtgcta attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc
     3841 ggcaatttgc attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa
     3901 ccttaaaagg tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac
     3961 agcgaggcca gtactggcgc ggcctgcagc gagatttacc actttgagag taattttttt
     4021 aactacgttt attgatctaa ctcaagaaaa tatcttcgga ctctggaaat tggtgtgata
     4081 actttgtcag catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg
     4141 actccgttta tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac
     4201 gactacgcaa aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt
     4261 gcggaagact atcgttttaa aaacctgtat gacatctggt tgaagggcga tcactacaaa
     4321 tggcgcgcta tgcgtaccaa cggtgtggct gagcgtctgt gtactggtga tgcgtctgac
     4381 cgtgaaaaat ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac
     4441 cactggacgc acctcgaact gcgtcgtccg ttcggtatca ctggcaaatt gctttctccg
     4501 tcaactgccg atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc
     4561 gcgcgcggca tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc
     4621 gattctctgg agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg
     4681 ccgagctggc gtccggacaa agccttcaat atcgaacagg cgacctttaa cgactacatg
     4741 gcgaagctgg gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc
     4801 ttgaccaaac gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg
     4861 gacgtggtga tgtttgctga agcgaacgaa gcggaactgg acagcattct ggcgcgccgt
     4921 ctggctggcg aaaccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg
     4981 ttccttggtg ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg
     5041 cgtaataaca acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc
     5101 aacgaccgtc cgatggcgga agagttgtct aagctgctga gcaagcagaa cgaagaaaac
     5161 ctgctgccga aaaccattct ctactgcctg aacccgcgcg ataacgaagt gctgggcacc
     5221 atgatcggta acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg
     5281 tggtttaacg atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt
     5341 ctgctgagcc gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt
     5401 cacgaatact tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa
     5461 gcaccggcgg acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg
     5521 cgtgactact tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga
     5581 tccatgcgct ggataacgtc gcggtcgctt tagcagattt ggctgaaggc acagaagtca
     5641 gtgtcgataa ccagactgtt acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt
     5701 taacggatat cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat
     5761 tggcggatat tgcggcgggg gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg
     5821 atctggatca gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc
     5881 ctgaagtgca gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaac gagctgtgga
     5941 tcctgccaac cgtgggctgt gtcaacggca tcgcgcggca aatccagaac cgtttcctga
     6001 aagagaccaa caacgccgaa ggtaccgacg gcgtgttcct cttcagccac acctatggct
     6061 gctcacaact gggcgacgat cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc
     6121 acccgaacgc gggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg
     6181 cattccgtga aacgttgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac
     6241 agcaggatga tgagatcgaa gccgggatcg agcatttgca tcagctgtat aacgtgatgc
     6301 gcaacgataa acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg
     6361 gttctgacgg tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg
     6421 tgattgctaa cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc
     6481 agttgctgat ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca
     6541 acgacttcaa acagtacttt attgcccatg atcagccgat ctacgaaaac ccatcaccgg
     6601 ggaacaaagc gggcggtatc accacgctgg aagacaaatc acttggctgt acccagaaag
     6661 cgggttccag tgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgccagggc
     6721 tgaacttgtt aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg
     6781 gctgccatat ggtgctgttc agtactggtc gcggcacgcc atacggtgga tttgtgccga
     6841 cggtgaaaat cgccaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg
     6901 acgcgggtca gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca
     6961 ccatcgttga gtttgccaac ggtaagcaaa cctgcaacga gcgtaacgac ttccgcgaac
     7021 tggcgatctt caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg
     7081 gcgtttcata atgtgaagcg ccgtttgctt ttccatacct gctaaggatt tatcatgacc
     7141 gcgtattggc tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt
     7201 ttcaatcgtg acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc
     7261 gtacattttt ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt
     7321 cgtacattga ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg
     7381 actggcggaa ttggcctcgc gaagttccat catcctgtcg aactattgcc ggttatcggt
     7441 acgattgtca gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg
     7501 tggttttcaa cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcacg
     7561 atgattgagg gtagttttct gcttatgaat ggcctgaata tcattcgttt ctggcggatg
     7621 cagaaaaggg gaattgatcc gtttaaagta gagaaaaccc ctcccgccat agacgaaagg
     7681 ggttaaacaa ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag
     7741 ctgccgcagt gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca
     7801 atacgccgat aataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga
     7861 acatattaca ggccagtggg atcagcagca aagaaccccc cgccacgccg gatgcgccac
     7921 aggcacacag agaagccact acgctcaaca gcagcgctgt gggcagatcg accggaatac
     7981 ccagcgtatt aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg cccgccatat
     8041 tgatggtggc tcccagcgga atagaaactg aataggtatc gcgatccaga ttcagctttt
     8101 cacacagcgc catattcacc ggaatgttcg ctgcagagct gcgggtgaag aaggcataca
     8161 caccgctttc gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc
     8221 acaccagcaa tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca
     8281 gcagttgcgc gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca
     8341 gcccaaaaat accgaacggt gcgaagcgaa tgaccagttt caccataaag gtaacggcat
     8401 tcgacatatc attgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaagccga
     8461 ggccgatcgc ccacaccaga atcccgatgt agttaccttt cagcagggcg tcgatggggt
     8521 tggaaaccat gctcattacc agtccgcgca tcacttcgac aatgcctgac ggcggcgaaa
     8581 tatcacccgc gctactggac aagtgcaggg tagaagggaa ggcaaagctg aagactactg
     8641 cggccagagc tgcagagaag gtgcccagca gatagaggaa caaaatagga cggatattgg
     8701 ttttctgccc gtgctggtgg ttagcaatag atgccattac cagcatcaac accaggatgg
     8761 gggcaacagc tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg
     8821 cgggttttga gatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta
     8881 ccaggctgcc atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc
     8941 gatcctttca ttgtgttgtc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat
     9001 gtaacaaagt gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat
     9061 gctggatttt aaagagaggt catgttttgc aacttaatgg ttgcatttat ttaacataaa
     9121 cagagggaag gactggctac taaggctgat tagataacat atgggcaaaa ctatttggaa
     9181 tatgagaacg gttgcgctcc tttaacgtgt tatgccatat aaagtcactg gcgcagggag
     9241 cgcgcagggg gcggccaatc gccgccgccc cctgcacccc caggctctgg cgaacaaaat
     9301 cgccgctgcg cggttccctc agcctatccc ttgcggctac cgggtcgggc gcaaggtaac
     9361 gtccctgtaa gatgcgccct cgccccggcc tccagggaac gcttcggcga ttttgacgcc
     9421 accaacaccc gtgcggtctt tttgtaaaga gctataagcc gattacataa ttaatagctc
     9481 acctttgtct ggaatgagtt atctctagga atggcttaat atacagcgaa tcattctccc
     9541 cgctgcttat catgccgata attcacccag gcgttgataa taaacgtcat caccagaatg
     9601 ccaaacacca cgcccagcga gacggcgatt ggaatatggt agaagtcgac aatcagcatc
     9661 ttgataccga taaacaccag aatcaccgcc aggccatatt tgagcatcga gaaacgctct
     9721 gctacgcccg ccagcaggaa atacatcgca cgcaggccga ggattgcaaa caggtttgag
     9781 gtcagcacaa tgaacgggtc agtggtcacg gcgaagatag ccggaatgct atccacggca
     9841 aaaatcacgt cgctcaattc caccagaatc agtaccagca tcagcggtgt ggcgtacagc
     9901 aatccattct tacgcacaaa gaaatgctcg ttgtcgatgg tgtcggtcat gcgcaaatga
     9961 ccgcgtagcc agcgcaccag cggcttgtcg ccaatgcctg attcatcttc atgggcgagc
    10021 gccatcttca cgccggtaaa cagcaggaag gcaccgaaga tatacaggat ccagtcgaac
    10081 tgcgaaatca gccagctgcc tgtgaagatc atgatggtac gcagaacaat cgccccgagc
    10141 acgccataca ccagcacgcg acgttgtaat gccgccggaa cggagaaata gctgaacaac
    10201 atcagccaga caaagacgtt atcgaccgcc agcgatttct caatcagata acccgtgaga
    10261 aaggccaggg cctgtggatc ggcaacggcg cgaccttcgg tttgcaccag ataccaccag
    10321 aaagcggcgt taaacagtaa cgacagcgtc acccagacca gcgaccaggc cgcagcctgt
    10381 ttcatggtca tggcatgtgc cccacgacgc ccctgcaaca acaggtcgat agccagcata
    10441 atggcgacaa caacagcgaa tccgccccat agcaacggcg tgccgacagt attcatagaa
    10501 gttccttaca cataaaaaaa cggccaacgt cggaagacgt cagccgctgc tttttatgca
    10561 tagacctcgc cttccggcaa ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc
    10621 ctttcgggtt gcccggtgac cggatgtggt ttttcacaca tcgtaatgac gatcgaccgg
    10681 caatgaagtt actccccttt gcaggtaaca aagtatgaca gaccattcag tcagtcaatg
    10741 gtctgttaca taccttttac actgttttac tttggcaagt atagtttacg ctatctgccg
    10801 gaaaaatcac cccagtttgg cggcggatct cggtcagcag tttggctgtg atgcgactga
    10861 ccgccagccc tggatgatcc accagatgct catccaccag ggtagcgaac agctctgctt
    10921 cgtagagcat ggtattaata tgctgcggct gggtgagatc ctgcatctgg ctgccacgcg
    10981 gcacaaagca gactttctgg cattcagaca gtttttcaat caccagcgat cccgcttcgc
    11041 cctgaatctc gcttcccagg acagaatcac tgacctttga gtgctgcaag gtgacgctga
    11101 aatcaccgta atccatcacc accacgccgt gggcgtcaac gccgcttgcc agcaaactcg
    11161 ccgttgcctg cacgcttttc ggctcaccaa ataacgccac cgccgacgtc aggcagtaaa
    11221 agccgatatc cataattgaa ccgttagaga atgccggatt aaaggtgttg ggattctcac
    11281 cgtccaggta acgttgatac cgcgaggaat actggcaata gttgaaaaag actttacgca
    11341 gtttgcctgc tttcggcagc gcctggcgca acaaatgaaa gttcggcagg caggcggttt
    11401 taaatgcctc aaacagcacc acctgatttt cgcgcgcaca ggcaatggcg gcatccactt
    11461 ccgccagatt cgacgccagc ggtttttcgc aaatcacatg aattttatgg ctaaggaaaa
    11521 gttgtgtctg ggaaaagtgc agggaattcg ggctggcaat atacaccgcg tcaatggcat
    11581 cgctttccgc catcgcttcc agcgacgtaa acagatgctc gacagaaaaa tcattggcga
    11641 agtgctgagc ctgctcaagg ctgcgggaat atacggcggt taacttgtat ttaccgctct
    11701 catgggcggc ctcgacgaac tggcgagtga tccagttcgt accaatcaca gcgaaacgta
    11761 tcataacgcg tggatactcc ataaagggat tctgtcgtca atgtagcacg cctttttcat
    11821 cttcgacgtg cgcagatacg cattattctt tcgccggaca gccatgctca ctaaacggcc
    11881 caatcatgac cgatggcccg agatatttcg gctggcgatg gaggattttt gcgtcaatca
    11941 ccgcgctgac acgagcgagt ttctcccgca gttgagtata aaaaccgtgc ttcatattga
    12001 ggtctggcgc gttaaacccg atagcgtcga tgccgtactg ttttgccagc cagatggcgc
    12061 gttggttatg aaattcttgc gagataatgg tgatatggtt ttccccaaac acctttttgg
    12121 cacgcactac cgaatccagc gttgagaatc cggcatagtc gcaaaagatc actttcgccg
    12181 gtacgccttt agcgatcaaa gcctgctgca tacctgatgc ttcatcgtaa tttttacgtc
    12241 cgttatcgcc actcaccagt agccatttca cttttccggc gtggtacaac gctgctgcgg
    12301 tatcaatacg tcgggtaaag tagcgattac ccggcctcgc ccccagcaat aaacccacgt
    12361 tacgcgccgg gacggcgttg acatcgcccc aggtcagctg cttgctggcg ttgaccataa
    12421 cgcgatcggc gataaaaatg gtcgctcctg ccacaagtaa cagtaagcag gcaatcttta
    12481 gagtccggcg agaaaaacaa atacggagaa gaaggcgggc aaatgcgcgc agcatggtcg
    12541 ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta aagaaaagat aacgcctgac
    12601 atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga
    12661 actcaagctg gtggtactgc ggctccatat ggcagcacaa ttgatagaac gctttgttat
    12721 gctctttctc cttcaggtgc gccagctcgt gcaccacgat catgcgcaaa aacggttccg
    12781 gcgcgttgcg aaacacggta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct
    12841 gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg atcttattgt
    12901 catacatcac tttattgatc ggcggggcat tacgcagaaa ctgatttttc agatcctgag
    12961 tatattgcca gagagcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca
    13021 gcacatcgcc cagacgctgt tcgttaatca gtgttcgcac ctgcgaaagc agctgctcgg
    13081 gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta
    13141 tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag
    13201 ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt
    13261 taacccgcta caggcgtggg aagcggcaga tgaatatttg ctgcaacagt tggacgacac
    13321 agaaatcccc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct
    13381 ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga
    13441 gaatttacgc ctaaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga
    13501 ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga
    13561 acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc
    13621 caaagcccgt gacattcaca cttccacgct ggaactgttc gaaaaagtgc tcggcccgac
    13681 caccaccacg ctggcatgga aaaaagcgcg cctgattaat tgcacgttca atgaaccgcc
    13741 gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca
    13801 taaccatgcg aatgtcttct cccgcaccgg gcttgatatc ggcgcgcgct tctttatgca
    13861 acatctgcca gagaatctcg aaggggagat tgtcgatctc ggttgcggta atggcgttat
    13921 tggtctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg atgaatcgcc
    13981 gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg cgttggatcg
    14041 cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct ttaatgctgt
    14101 gctctgcaac cctccgtttc accaacaaca tgcgctgacc gataacgtcg cctgggagat
    14161 gttccactac gcccgccgct gcctgaaaat caacggcgag ctgtatatcg ttgccaaccg
    14221 ccatctggat tacttccata aactgaagaa aattttcggc aactgcacca ctattgcgac
    14281 gaataataaa tttgtggtgc tgaaagcggt gaagctggga cgtcgtcggt aagattggga
    14341 gttacctgcc ggatgcggcg taaacgcctt atccggccta cgttcagcac gttaccccta
    14401 ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgctcagttc tggaatcgcg
    14461 cttccataaa acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgtttttc
    14521 tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt
    14581 atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca
    14641 ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctggggaac
    14701 acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg
    14761 gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt
    14821 gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat
    14881 aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg
    14941 ccgacatctt gcaagcagga gaaaagctaa ctgctgtggc accttttctg gcgggtattc
    15001 agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc
    15061 ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc
    15121 ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc
    15181 gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat
    15241 ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat
    15301 tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg
    15361 cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt gttccctggg
    15421 caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcacag
    15481 ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga
    15541 tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt
    15601 catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcagga
    15661 tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc
    15721 gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc
    15781 caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct
    15841 ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac
    15901 aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca tcgagataac
    15961 tcaatacctt cggatgatcg atcccgtcga taggcggaac acgcggcacg atcccactgg
    16021 cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca
    16081 gcgtcacgcc cgtcacttcg atcatccggc ggtagtagcg cagcgtttcg taaaactcct
    16141 ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgcgcatcaa
    16201 acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc
    16261 cagcgccgac caccgccaga ttttttttct gcacggcggg aaggattggc attttggttt
    16321 cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga
    16381 ggcagccctg gttgcagcca atacaagtgt tgatctcatc ggctcgccct gattgcgctt
    16441 ttgacagcag ttccgcatcc gcgagaaacg gtcgcgccat cgatactata tcggcatcgc
    16501 cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gttaccagcg
    16561 gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg
    16621 gcgtggcgat ggtcggaatc cgcgcttcgt gccagcctat tccggtgttg ataatggtcg
    16681 cgcccgccgc ttcaatggcc tgcgccagtt ctaccgtttc ggcaaaagtg ccgccgcctt
    16741 cgaccaggtc gagcatcgac aaccggtaaa taataatgaa gtcgttgccg acgcgttcgc
    16801 gcaccgtacg cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact
    16861 ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcagagc
    16921 ccatcacttc gacgccatcg tatccggcct cccgcgccag ttgcgcgcag cgggcgaaat
    16981 cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg
    17041 gggcctgcaa tgcggacggt gcgaccagat gcggttggta gctgtagcgc ccggtatgca
    17101 aaatttgcag ggcgattttg ccgccttcct gatgtacggc ttcggtaatg gtgcgatggt
    17161 gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat
    17221 ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa
    17281 acgcagccag ccgttcagca ccgtccgggt attcctccag cccggtgtgc attgaaccca
    17341 tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt
    17401 agctcataaa attgtccagt atgtaaaata attgttatgt agtcggatga gttctaattt
    17461 agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga
    17521 atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc ttgggggatt
    17581 gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agccgttgca aattactgct
    17641 tacggaaaaa atcgttatac aacatataca aatgagcggc actccaggag aaatttggtg
    17701 cgccttgctg cgcgcctgtc agcgggttgt aattctcctg aattgggcca tcggcggtta
    17761 atcctttggc gtgcctgaag aacgtatccg ccagcttcag ggcatcatca cgataaccgt
    17821 aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccgcgccagt
    17881 agatatcagc gccaaaagcc ggattggtta acgccgccgt tcccagcgga acaaaggtgt
    17941 tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg
    18001 caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc
    18061 ccgcgcagcc gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag
    18121 tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc
    18181 gtttggcctc ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc
    18241 tgtacatata gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc
    18301 cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag
    18361 cgacatattt atccaactgt tctttgtcga taaaaccaaa gacggcggca tcatcacggc
    18421 ccgattccca cgacgcagcg acctgtgccg gaatttccag actgtcgtac tggcctttct
    18481 ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg ccttttttca
    18541 ccgtaaacag catctcgccg ctctcggtgt tgtgggcttt gtcgcgggtc gcgccatatt
    18601 ccggcacccc gttgccgtta tgatcgcggt tacgtaacca ccagtcgtga taggccacca
    18661 gtttcgggta catctctgcc aaccaggctt tatcctgggt gacgttgtat acttccatca
    18721 ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac
    18781 cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac
    18841 gcacgctgtc gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga
    18901 tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct
    18961 gattgccgga gaaccagcgt ccggtcaccg acggcgtgac ggtgttataa ttcaccgcac
    19021 cgcccggtga gcgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct
    19081 gttccggcgt cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct
    19141 gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt
    19201 tgctaacttc ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt
    19261 tgatatgcgc cttactggta aagcgattac ggttgatttc agtctgcacc ggcagggatt
    19321 tatgcacctg atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc
    19381 caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt
    19441 cgccagcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca
    19501 gttcgccatc ccacaccaga tccagcggtt tattgctgat gattttagtt tccagtagtg
    19561 acgtgcgcgg cgtggcgaag cgcagagtca tttcgacctg cacatctttt gctgtcagtt
    19621 tttgcaccag cgcgccggga atactgtatg cctccagcgt gaagttgact ttcttgccgt
    19681 cttgccagac agtcaggcgg tcgaaattgc tggccataaa gttgatgtat tcttccgtca
    19741 gcagcgcaac gcccggaaag ccgcccatgg tgttcgggcc gtctggcaac agatgaccat
    19801 gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct
    19861 tcatgtactg cggcgcacca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg
    19921 catgagcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata gttttgattt
    19981 tcatgagatt acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg
    20041 ggatcgttag aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg
    20101 ccgccatatt cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg
    20161 tcagggcgat gtttggcgta ggtgtaagcg gtggagagat aaacgccttt taacgcttca
    20221 tacatggcgc tcaccatcat cccgctttca cttcccggta cgtccagccc gtcggcacgt
    20281 ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa acaggcccgt gagatagagt
    20341 tgtcctgtgc cgtcggtaat ttccagacca ttcatccagg tgatgccttc ggcaaggtca
    20401 tactggatgt aaccgttcat ccattgccgg ataggtgtat ttgtcgtcgt agcgatcgta
    20461 cttgccaaaa tgtagccagg ctttgctttc gtcatgatgt ccggcaggtg tggcggtgac
    20521 gctataacga aagttgccgc ttaagttctg taccttcagc gcggtcataa tgtcgcgggt
    20581 attgggaatg acgtagccca aatcgggtgt gaagtcgccc caccattgca ggtcatccag
    20641 cgaggaatcc tgacgcagac tgagcatgat ttcggtgcca tcattggtac gataaccgcc
    20701 atagaaacgg ttaatcccac cttcgaaacc accccagcta tggtcaggca cctaggcatt
    20761 accgtcgtgg tcggcctgca cggtccagcc ttcgccgtaa atcaggccga accaggagcc
    20821 gtacttcatc tccaggccac cttcaatata ggtgccgtcg ctgtatttat gtttattctc
    20881 accctctaaa tccatatatc caccaagccc taattcgccg taaaaacgaa atgaaggaga
    20941 agatgtcgag gtaattgcgg gtgtttttgt gtcgtcatgt tctgccggac gtgcttcttc
    21001 tcctgccgca gaaaaggcgt agcaaaacgg cagagcaagt aatgctctgc atggtgcagt
    21061 gattaatttc attgtgttaa tccatgagct aaagaaaaag tacagccttt tatcgttgta
    21121 attataaaaa tataaaggca gaaactattt tccctctcct catccaggaa gagggaaaat
    21181 gcctgctcat tttttattag tctgcttaat caacattgct ggctggcgtc gcttcaatat
    21241 ggcgctcttc agccgttaat ccctttatat atttgctgta tttccaccac gcaaagccga
    21301 ggaagataac aataccgccg acgttataaa aaatgatggt cagaatattc gcgccagtcg
    21361 ggaatgtcga agcgacaaac cctacggcaa atatcgcaat cagcattgaa acaacgataa
    21421 taccggtgcg gcgggagccc atacggaaat cgcgtggcag gtgatctaat ttggcgcgta
    21481 aattcaggta agccagcatg atgaataacg gcggaagcat ggacgctgcg gcggtcatat
    21541 taataatggt attcatcaga tcctgcacgg tattggaacc gagcatcggg ataatcatca
    21601 gcgggatgac gatcagaaac tggatccatg ctgcgcgcgc cggaacgccg ttttcgttaa
    21661 gttcgacggt tttcttacca aagatgcctt ccgggatttc ggagaagaaa attttcaccg
    21721 gcgttgcggt ccacatcagc agggaaccga acattgcggt aaaggacacc agaccgacaa
    21781 agcgattcat caacgcttcc ggcagaccaa aatacgccgc catgccgtgg aatacctgca
    21841 ccgatccgcc ggtaaatttc aactctttgc tgctgacgaa gacgttaatc agcaccgagg
    21901 agacggaata cagtacgccg ataaaaatcc cggcgaggat gatcacttta acgaacgatt
    21961 tcgaaccgcc tttgacgtcg ttaacgtaca ccgcgacgga ctccgcgcca cctgcggcca
    22021 taaagatcca ggtggtaacg ccgaggaacg cccagttgaa gttcgggatc atcgcgtcaa
    22081 cggtgatcgg atcggcaggc tgtacgccgc caaccagtgc cgtacctgcc agtaaaatgt
    22141 aggagagcgt taacagcagc atcagcgttg aagtgacgga ggtaattggc cccagcattt
    22201 tcgccccgtt ggtggaaacc caggtggaga aggcgaacag caccatactg ataacggtgg
    22261 tggcaaccgg cgtcataata tattcgtagc cgaggaaggc atacgaagca taagcaataa
    22321 cgcgcggcaa cagcgaggtg aaaaagaaca gatttacgaa ccagtaagtg taggcagtaa
    22381 taaatgccca acgtccgccc agcgaacttt ttacccacgc gtagacaccg gcttctgagt
    22441 ttttatttaa cgaaacaaat tctgcgatga tcagacagaa gggaataaaa taaaaaatcg
    22501 tcgcgaggaa aaacatcggt gccgaggcca gtccaagctc aatattatta ttgataacgt
    22561 tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa gccgaatttg ccgattgtat
    22621 tacgtttggt atcagacata acacatctcc gaaaatgtgt agggtagcgg tggcggcaaa
    22681 ctccttttgc cgccgtagtt gttatttgtt atggaaataa ccatcttcga tggtgacttt
    22741 gagaaccact tttttgaccg cgttattgca gataaaccga tacgcttcat ggatatcgca
    22801 aataacgatt tgcccttcgt ggacctctac ggtttctccg cagcctttta aatattcacg
    22861 gtcagtttca tcacgataat attccactac ctgtaatgtc tctttcggcg catattcaat
    22921 tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag cgtcgatgcc cggtaaatag
    22981 cgcatcggtc gcggagtcat tttccacacg gtaggtcaat gaatcaccga tggagtgggc
    23041 gacgccaggc tgaatgttgt cgatattttc aatcgcttca acgcagcgtt gccacttctt
    23101 gccagaggcg taaatctggc ggaactgttc taagttatcg atgatcctca ttgcttattc
    23161 tcgctgtgca aattcgtgga aaagaaccct gcgccgaact catacgacgc caggctttgc
    23221 gcggtagctt ctccgccaga aaccggcagc aacgtaaagc cgtagctgaa gtcacggaac
    23281 cagacgcgcc aggagtccag cacctcgctg ccccaggagt tggagccgag gccaagtagc
    23341 tggtggtcga ggttcagggt gatgtcatca ctgcgctgca gctcgttaca gtgctgggca
    23401 gcgtggatgt tttcctgggt atagtgccag gcgctgaagt taattgggcg ctgcggaacc
    23461 accagcagac cgttgccgtg gcggttagtc agtgccgtcc agcggacatg ctgacggttg
    23521 ccgttgttct gcgggaaggg atagttctcg aacatggcat cgacggtgct gcgccagata
    23581 tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc ccggtccacg accgtaatac
    23641 gccacctgat cgtattcgcc gttaatcccc atggtgaaac cgatgcacgg aatgatgtgc
    23701 ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa cctggccatc ggcagcgatg
    23761 cgccagatgt aggtacagcg catcccgaag tcaaacaccg gcggtgcaat aactgtgcgg
    23821 ctgatgatca acacttcacc atcgctctgt tctacggcaa agtcgcgcag atgttcctgc
    23881 atgatctgca aatgattcgg ctgccacaac ccttcgtact cctgcttgtg gttgtcgatc
    23941 atcggcttga agaagttgat ctttggctcg cgggtcagca gcgactcgcc atttacctgg
    24001 caggatgtcg gtttgccact catttttgag aaggtgatcg cgaagttgta gccgcgaacg
    24061 gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg cattatttgg tgcgaaaggc
    24121 actggctgcg cggtgttttc cttcagcggg aactgataag aggcgataga atgcccggct
    24181 tcgctgtagc gggtgcggga atctttggtc accgtaatgt tgaggaacgc ttcgcgggcg
    24241 tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt tcggcgcaac gtcgcgcagt
    24301 ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca cctctgcgtg cagggtgtag
    24361 tcatcaagcg tggtaaacca cagtttattt tcgactttca actcgccgcg agtcagcgcg
    24421 tggattttta ccggcgcgat aacctgtttg tactctttca ggcccgatcc cggcgtctga
    24481 tcggaataga tcaaaccatc aagacagaag ttatagttgt tgggatagtc gccgccgaat
    24541 ttataccaga cattgccgtg gtcgtcctgt gcctggatcc cgtggtcgca ccactcccag
    24601 acataatgac cctgaatgca atcgtgctta tagaagacgt tctggtactc cgtcagcccg
    24661 cccggtccgt tccccatcgc atgagcatat tcacagatga tgcgcggctt cggatgcggg
    24721 tattcaccaa actcattcat cagcggcacg cgggtgtaca tggtggaaat aatatcgacc
    24781 acttcagcat cgcgatcttc ttcgtaatga accagtcgcg tgtcatccag cgctttcgcc
    24841 gcatggtaca tcgcgcggat gttacagcca tagccggatt cattgcccag cgaccagatg
    24901 atgatcgacg gatggttttt ctgtgcgtgg atatggcgaa caatgcgctc gacgtagact
    24961 ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc cgacattagc aaagccgtgc
    25021 gattcgacgt cggtttccgc catcacaaac aggccgtaga tatcacacag ttcgtaaaaa
    25081 cgcggatcgt tcgggtagtg agcggtacgc acggagttga tattgtgctg cttcatcaac
    25141 tggagatctt tctcgacgcg atccattcca acggcgcggc ctttgcgatg atcgttgtcg
    25201 tgacggttga cgccgtgcag catcacataa cggttattga tccagaacag accgtcgcgc
    25261 actttgatat cacggaagcc aacgcgttgt ggcaccactt ccagaacgtt gccgttggcg
    25321 tctttcagcg tcatgaccag atggtaaaga taaggggatt ctgctgacca ttgctgcggc
    25381 tgttcgacag taaaagcaaa gctggcgctg gtcagttttt caattgccaa atgatcaatg
    25441 gcgctgctgt gcaccacgcg ttcgccatca aacagggtat attccagcgt cgtgacgaca
    25501 ggggaggcgg cgagattttc cagcaccact tcgcaggaaa gcgtggcatc gcaataggct
    25561 tcgtcaaagt cggtacgcac agtgaaatcg ttaatatgcg ttaggtgttt tccgaccaga
    25621 taaacatcgc ggaagatccc cgccgaccac cacatatcct ggtcttccac gtaggtagag
    25681 tccgcccact gcatcacgcg cacacacaac aggttgtcgc cggttttaac catcgcgctg
    25741 atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac ccacatactg accgttaacg
    25801 tagacttcaa aataggtttc gacgccgtca aatttaatca gcgtctgttt accctgccag
    25861 ccgtcgctga gggtgaaaat acgttgatag gcaccggttg ggttatcgct ggggacaaac
    25921 ggcacatcga tggggaacgg aaaaccttcg tcggtatatt gcagtttgcc gtgaccttcc
    25981 atttgccaca tggcggggac ggtaatatgc ccccagtcag ccattaactc agaggtgaag
    26041 gcttctggta cttgcagcgg atggtcaaaa aagtggaaat tccactgact gcttaagggc
    26101 agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt gcgcaacaga atcatatgaa
    26161 aaaaagtacg cacgcggcgc aagtcggttt tcgtgggtga gctgaatgtt ttcccagcga
    26221 ttcatagggc ctcccggtag agagaaatcg gaaccattca tggtgatggt agtttgtgta
    26281 attgctgggg ggttagtaaa atttttacta aaaagcagca ggtaaatgca tttactttaa
    26341 ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa tgattgccgg acagtgtgta
    26401 aggggattta acgggtcgtg ccgcgcagtt ttaatttgct gggaacgaag actagcagcg
    26461 gcagcgcgcg accatcgcgg gctttttcat acaccaggtt aacgccctga cttcccatca
    26521 tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt aaatcgcgcg gtggggatat
    26581 cgttaacgct gataagcgaa atatcttgtg ggatgttcag gccacgttca tgaattgccc
    26641 gcagtacgcc gatagcaatg gaatcggaag caacaaacag tgccttcgga tagtcttccc
    26701 gcgccagcat ttgttttgcc agttcataac ccgacgaact ggaaaaaccg ccgcgccaga
    26761 tatcctcttc gcgtaccact tgtttcagtc ggccatattc cgcaaaggcg acctcacgaa
    26821 tatccgcctt gccaggctca tcttcaccgc caataaaacc aatacgatta acgccctggt
    26881 tgatatagaa gtcgatgatt tctttactga tgcgtgccag atcgatatcc accgcatcgt
    26941 aaccgctgcc gggttcgtga aagtcgataa aacagatatt gtcggtcaac gcgctggcag
    27001 cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat accggtgacg tttttaatgt
    27061 ctggtaatcc gttgtgttca taacagttgg tcagttcgat cgccagcttt tcgcactgag
    27121 tttcaatgcc gtggcggatc gccagatagt aaggatcgtt gatctccagc tcctgctggt
    27181 agctgtagat agccagaata tggtgttggt tgactgcgcc tgtctgtagt ttacgggcgc
    27241 tactggtctt gtactccagc ttttcggcga tctcgagaat gcgatgtttc gtctcttctt
    27301 tcacattcaa tgtcggatcg tcatttaaga ccctggatac tgtcgccagg gatacgccag
    27361 cttcgattgc gatgtctttt aatgttgcca tgtttacctt ttttactttc cctgatgtgc
    27421 gcccccattg tagaggaagt gcttatcggg tcacctgttt tcagtaaata aagcgcgttt
    27481 ctttgacgct gtaacggtaa cacagcggct taggaatgtg atcacgcctg cattctccat
    27541 ttttagccta caggattgag aaaaactttt ctatagttta gtaaaatttt atccgaaggg
    27601 aggaagcaat ggaaaccgtg gcttacgctg attttgcacg tctggaaatg cgcgtcggaa
    27661 agattgtgga agtgaaacgc catgaaaacg ccgacaagct gtacatcgta caggttgatg
    27721 tggggaaaaa acactgcaaa ccgtgaccag cctggtgccg tactacagcg aagaggagct
    27781 gatggggaaa acggtggtgg tcttatgcaa tctgcaaaaa gcgaaaatgc gtggtgaaac
    27841 gtcggaatgc atgctgttgt gtgcggaaac cgatgacggc agtgaaagcg tgttgttaac
    27901 gccggagcgg atgatgcctg cgggcgtacg cgtcgtataa aaagatcgga tggcgacgtc
    27961 gtatcgccat ccgatttgat attacgcttc ttcgacactt actcgcatgg ccgccagcgc
    28021 cttacgcgcc gctttgatca ccagttcaca ctgttcaatg gtcagtgtca gtggcggttc
    28081 aatgcggatc gttttggcgt tattgagcgt tccggccacc agtacgcgct ggcggaacat
    28141 ctcgctggca aagttatagc cgatttcgtt atcaacaaac tcaatcgcca tcaacatccc
    28201 tttaccacgc gcttcctgta ccagatcggg atattcccgc gccagttgac ggaaaccgtc
    28261 cagcaacata tcgccttttt gctcagcctg agccggtaag ttctgctcca gcaacacatt
    28321 gatggtcgcc agcgccgccg cacaggccag cgggttgccg ccaaaggtgg tggtatgcag
    28381 gaatgggttg tcgaacagaa ctgaaaacac ctcttcagtg gcgatggtcg cgccaatcgg
    28441 catcacgccg ccgccgaggg cttttgccag acacagaatg tccggctgaa cgttctcatg
    28501 ttcgcaggca aacatcttgc ccgtgcgccc catgcccgtt tgtacttcat cgaggatcat
    28561 cagtgcgccg aactcatcgc acagcttacg cacggcggtg agatagcccg gcggcggcag
    28621 aattacgcca ccttcgccct gaatcggttc gaggatcacc gcagccacat catcaccggt
    28681 ttttttgcac tcattaagag ccgtgcgcat ggcttcgata ttgccaaacg gcacatgacg
    28741 gaaccctggc agcagtggca taaacggttt gcggaaggtc gatttcgccg tggccgacag
    28801 cgcgccaagt gatttaccgt ggaacgcgcc gctggtggca ataaaagtaa atttgccgcg
    28861 cggcgactga taagccttcg ccagcttcag cgccgcttca acggactcgg tgccgctatt
    28921 acagaagaag ctgtatttca gtttaccggg cgttagcgca gcaagggttt tcgccagcat
    28981 agcccgtaac ggatcgagta gctcctggct gtgcagtggt tgtttcgcaa gttgattctg
    29041 tacggcggaa accacaactg gattacggtg ccccacgttg aaaattccaa aacctcccag
    29101 gcagtcgata aactcctgtc cctgggtgtc gacaagcgta tttaaacctc ccgcttgcca
    29161 ctctacggct ccgtaatccc cgccggcggt aacagatttg cgatactcta aaaaccccgg
    29221 attgacatgc tctttgaagt attcaatcac ctctcggtta agtgctttca tctcctcatg
    29281 atccagcgtt cgcttctcaa tgagattcag ggcgtgggcg ctgcacgcta aagccgatgc
    29341 gctcgaaggt aacctgttca aaatatgctc cggaggctcg cgtatcacat gataccgatt
    29401 taagtattgc agggattgcg ccactccggc tgcgatcgcg aaaatcggga taaacacaaa
    29461 tgcggttatc atttgtgcaa tatttaatgc tatccgttaa catttgttgt tattttgcac
    29521 ggtttttgtc gggtatggat ttgatgattg ctgcgcaaat gcacgttttc agggcaataa
    29581 ttgcctgatt tttgggcaag ataattaacg ttttcgtata gtaacagagt gaaagtattt
    29641 taaatcaaag aattagattt gtgctttaat cgcaaattgc gatctaaatc aaattaatcg
    29701 gttaaagata accgcagcgg ggccgacata aactctgaca agaagttaac aaccatataa
    29761 cctgcacagg acgcgaacat gtcttctcat ccgtatgtca cccagcaaaa taccccgctg
    29821 gcggacgata ccactctgat gtccactacc gatctgcaaa gctatatcac tcatgctaat
    29881 gacacttttg tgcaggtgag cggctttacc ttgcaagagt tacaagggca gccgcacaac
    29941 atggtgcgtc acccggatat gccaaaagcg gcgtttgcgg atatgtggtt caccctgaaa
    30001 aaaggggagc cctggagcgg catcgtgaaa aatcgccgca aaaatggcga ccattattgg
    30061 gtgcgggcca atgcggtacc gatggtgcgc gagggaaaaa tcagtggcta tatgtcgatt
    30121 cgtacccggg cgacggatga agagattgcg gcggtggagc cgctgtacaa agcgttgaac
    30181 tccggacgta ccagtaagcg tattcataaa ggcctggtgg tgcgtaaagg ctggctgggt
    30241 aaactgcctt cattaccgct tcgctggcgg acgcgtggag tgatgaccct gatgtttatc
    30301 ttgctggcgg ccatgctttg gtttgttgct gccccggtgg tgacgtattt cctctgtgtg
    30361 ttagtggtat tgttggcaag cgcttgtttt gaatggcaga ttgtgcgccc gatagaaaat
    30421 gtcgcccgtc aggcactgaa ggtggcgacc ggagagcgta atagtgttga gcacctgaat
    30481 cgcagcgatg agctggggct gacattacgc gcggtagggc agcttggcct gatgtgccgt
    30541 tggctaatta acgatgtctc aagccaggtg tccagtgtca gaaatggcag tgagacgctg
    30601 gcgaaaggca ccgatgaact gaacgaacat acccagcaga cagttgataa cgttcagcaa
    30661 acggtggcga ccatgaacca aatggcggcg tcggtgaaac agaactctgc cacggcgtcg
    30721 gctgccgata aactttctat caccgccagt aatgcggcag tgcagggtgg ggaggcgatg
    30781 accacggtga tcaagacaat ggacgatatc gccgacagta cctagcgcat tggcaccatt
    30841 acttcgctga ttaacgatat tgcgtttcag accaatattc tggccctgaa tgcggcggtg
    30901 gaagcggcgc gtgccggcga acagggcaaa ggttttgcag tggtggcagg ggaagtgcgt
    30961 catttagcca gccgcagcgc taatgctgcc aacgatattc gcaagctgat tgatgccagt
    31021 gctgataagg tgcaatccgg ttcgcagcag gtacacgccg ccggacggac gatggaagat
    31081 attgtggcac aggtgaaaaa cgtcacccag ttgatcgccc agattagcca ttcaacgctg
    31141 gaacaggccg atgggctttc cagcctgacc cgtgcagtgg atgagcttaa cctgatcacc
    31201 cagaaaaatg ccgagctggt ggaagagagt gcgcaggtgt cggcgatggt gaaacaccgc
    31261 gccagccgac tggaagacgc ggtgacggtg ctgcattaat cggtatgccg gatccggcgg
    31321 ttggagcaca atgcctgatg cgatgctggc gcatcttatc aggcctgcgg ggtgtgtagc
    31381 gggtgtaggc cggataaggc gcgtcaagcg tcgcatccgg caattgcacc gcgccactgg
    31441 cggatgcggc gattaatcca gttgcgtaat atcaaaagcg gcgcggtcga tcaccgcaat
    31501 gatctttttt atttgtgcat cactgatatc gctctggttg acgcgtaaat ccagcaccgc
    31561 tttaaaatta tccagcgccc gcttcatttg cgggttctgg cgcagcgccg cgccaacgca
    31621 acgcgctttg atgcgttctt caatcatctc cacttgttcg cggttttctt ccagccactg
    31681 cgcaccttgt tcggtcagcg caatctgctt cttaccacct tcctcttcgc ggatggtaat
    31741 cagcgactgc tcctgcagaa aatccagcgt cgggtagatg acgcccgggc ttggggtgta
    31801 attcccctgg gttagattct caatcgcttt aatcaattcg taaccgtggc tgtcatcgcg
    31861 cgagagaata tccagaatca ccagacgcaa ttcaccgtga ccaaagaaac gttgccgacg
    31921 accgccgccg cgaccgtggc gaccaccgca acattgaccg tgttcatgct ggtgaccgtg
    31981 tccgcagtgc tcgtgttctg acttctcacc tttgcagcag ccctcatgcc gtggctggcc
    32041 ttcatgttta caacaccctt cgtgatgatg gctcatatca gcctcctttt gcttgattag
    32101 atatatctaa tttatatctg atgtgagcat tactgcaagt acaaaaatga tttttaaacg
    32161 tggaagcacc tcacatctag gccggataag acgttcacgc cgcatccggc agcattgcta
    32221 tggcactgaa tgccttcaca tacttcagac atatcagttc tacaaatcgc ttgcatttat
    32281 catgattaac aatcattatc atttgcgaat tttatttaga tatatctgat taccatcacg
    32341 aaggcgataa caatgaataa caccccccgc tacccgcagc gcgttcgcaa tgatctgcgc
    32401 ttccgtgaac tgactgtgct acgcgttgag cgcatcagcg ccggttttca gcgcattgtc
    32461 cttggcggcg aggcgttgga cggttttaca tcgcgcggct ttgacgatca cagcaaactc
    32521 ttctttcctc aacctgacgc tcactttgtg ccgccaacgg taacggaaga gagcatcgtc
    32581 tggcaggaag gaccacgccc accgtcgcgt gactatacgc cactgtatga cgaactacgc
    32641 catgaactgg cgatcgattt ctttattcac gacggtgggg tcgccagcgg ctgggcgata
    32701 aacttacggt ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac gcgtatcagc
    32761 tgtatgtctg cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa acgttgagca
    32821 aacttgccgt taaaccgcaa gttagtgcgc tggttagcgt gcgggataac gcctgtcagg
    32881 attatctcgc gcaccttgat ggttttaata tcgaatggct ggcacatgat gagcaggcgg
    32941 tagatgcgcg tctggcgcag atgcaaatcc ctgccgatga ttacttcatc tggataaccg
    33001 gcgaaggtaa agtcgttaag aatttaagcc gccgctttga agcggaacag tatgacccac
    33061 agcgggtacg tgcagcggct tactggcacg caaagtaact gatatataaa aagaaaaagg
    33121 ctgacgattt ctcgtcagcc tttgctatat ctggtggccc ctgctggact tgaaccagcg
    33181 accaagcgat tatgagtcgc ctgctctaac cactgagcta aggggccatg gtagcggatt
    33241 ataaagtaac tccgtgtcgc aatccagcca ttaccgcgcg cctgctgttt ttataaacaa
    33301 tgtattttca atccgttata cttttcctgg tgatgttaga aaggagtaaa tatggttgag
    33361 gatattttgg ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc
    33421 gccgggactg gttttccctt tgctcatccg gcaaatcgct tctggaaggt gatatatcag
    33481 gccgggttta ccgaccgtca gttgaagccg caggaggcac agcatctgct ggattatcgt
    33541 tgtggcatca ccaaactggt agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag
    33601 gagctgcacg caggtgggcg taagctgatt gaaaaaattg aagattatca gccgcaggcg
    33661 ttggcgattc tgggcaaaca agcatatgaa cagggattca gctagcgcgg tgcacagtgg
    33721 gggaaacaaa cgctcaccat tggttcgacg cagatttggg tgctgccaaa tcccagcggt
    33781 ttaagtcgcg tttcactgga gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg
    33841 gtagtgcgtg ggcgataaaa aacgtcaccg g
//
LOCUS       DAECNO010000038        33546 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.038, whole genome shotgun sequence.
ACCESSION   DAECNO010000038 DAECNO010000000
VERSION     DAECNO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.038"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            78..1355
                     /gene="waaA"
                     /locus_tag="KKO08_001849"
     CDS             78..1355
                     /gene="waaA"
                     /locus_tag="KKO08_001849"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBD6318118.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1363..1842
                     /gene="coaD"
                     /locus_tag="KKO08_001850"
     CDS             1363..1842
                     /gene="coaD"
                     /locus_tag="KKO08_001850"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBD6318119.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1881..2690)
                     /gene="mutM"
                     /locus_tag="KKO08_001851"
     CDS             complement(1881..2690)
                     /gene="mutM"
                     /locus_tag="KKO08_001851"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBD6318120.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2788..2955)
                     /gene="rpmG"
                     /locus_tag="KKO08_001852"
     CDS             complement(2788..2955)
                     /gene="rpmG"
                     /locus_tag="KKO08_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBD6318121.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2976..3212)
                     /gene="rpmB"
                     /locus_tag="KKO08_001853"
     CDS             complement(2976..3212)
                     /gene="rpmB"
                     /locus_tag="KKO08_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBD6318122.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3429..4097)
                     /gene="radC"
                     /locus_tag="KKO08_001854"
     CDS             complement(3429..4097)
                     /gene="radC"
                     /locus_tag="KKO08_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBD6318123.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4269..5489
                     /gene="coaBC"
                     /locus_tag="KKO08_001855"
     CDS             4269..5489
                     /gene="coaBC"
                     /locus_tag="KKO08_001855"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBD6318124.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5467..5925
                     /gene="dut"
                     /locus_tag="KKO08_001856"
     CDS             5467..5925
                     /gene="dut"
                     /locus_tag="KKO08_001856"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBD6318125.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6032..6628
                     /gene="slmA"
                     /locus_tag="KKO08_001857"
     CDS             6032..6628
                     /gene="slmA"
                     /locus_tag="KKO08_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBD6318126.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6665..7306)
                     /gene="pyrE"
                     /locus_tag="KKO08_001858"
     CDS             complement(6665..7306)
                     /gene="pyrE"
                     /locus_tag="KKO08_001858"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBD6318127.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7372..8088)
                     /gene="rph"
                     /locus_tag="KKO08_001859"
     CDS             complement(7372..8088)
                     /gene="rph"
                     /locus_tag="KKO08_001859"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBD6318128.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8215..9078
                     /locus_tag="KKO08_001860"
     CDS             8215..9078
                     /locus_tag="KKO08_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBD6318129.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9299..10123
                     /gene="dinD"
                     /locus_tag="KKO08_001861"
     CDS             9299..10123
                     /gene="dinD"
                     /locus_tag="KKO08_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBD6318130.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10414..11031
                     /locus_tag="KKO08_001862"
     CDS             10414..11031
                     /locus_tag="KKO08_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBD6318131.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11028..12710)
                     /gene="ligB"
                     /locus_tag="KKO08_001863"
     CDS             complement(11028..12710)
                     /gene="ligB"
                     /locus_tag="KKO08_001863"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBD6318132.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12968..13591
                     /gene="gmk"
                     /locus_tag="KKO08_001864"
     CDS             12968..13591
                     /gene="gmk"
                     /locus_tag="KKO08_001864"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBD6318133.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13646..13921
                     /gene="rpoZ"
                     /locus_tag="KKO08_001865"
     CDS             13646..13921
                     /gene="rpoZ"
                     /locus_tag="KKO08_001865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBD6318134.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13940..16048
                     /gene="spoT"
                     /locus_tag="KKO08_001866"
     CDS             13940..16048
                     /gene="spoT"
                     /locus_tag="KKO08_001866"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBD6318135.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16055..16744
                     /gene="trmH"
                     /locus_tag="KKO08_001867"
     CDS             16055..16744
                     /gene="trmH"
                     /locus_tag="KKO08_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBD6318136.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16750..18831
                     /gene="recG"
                     /locus_tag="KKO08_001868"
     CDS             16750..18831
                     /gene="recG"
                     /locus_tag="KKO08_001868"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBD6318137.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18865..20070)
                     /gene="gltS"
                     /locus_tag="KKO08_001869"
     CDS             complement(18865..20070)
                     /gene="gltS"
                     /locus_tag="KKO08_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBD6318138.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20350..21741
                     /gene="xanP"
                     /locus_tag="KKO08_001870"
     CDS             20350..21741
                     /gene="xanP"
                     /locus_tag="KKO08_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBD6318139.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21862..23571
                     /locus_tag="KKO08_001871"
     CDS             21862..23571
                     /locus_tag="KKO08_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBD6318140.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23624..25942)
                     /gene="yicI"
                     /locus_tag="KKO08_001872"
     CDS             complement(23624..25942)
                     /gene="yicI"
                     /locus_tag="KKO08_001872"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBD6318141.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25952..27335)
                     /locus_tag="KKO08_001873"
                     /pseudo
     CDS             complement(25952..27335)
                     /locus_tag="KKO08_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27628..27722
                     /locus_tag="KKO08_001874"
     tRNA            27628..27722
                     /locus_tag="KKO08_001874"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27662..27664,aa:Sec,seq:tca)
     gene            28023..29207
                     /locus_tag="KKO08_001875"
     CDS             28023..29207
                     /locus_tag="KKO08_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6318142.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29619..30662
                     /locus_tag="KKO08_001876"
     CDS             29619..30662
                     /locus_tag="KKO08_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBD6318143.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31177..31605
                     /locus_tag="KKO08_001877"
                     /pseudo
     CDS             31177..31605
                     /locus_tag="KKO08_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31635..32786)
                     /locus_tag="KKO08_001878"
                     /pseudo
     CDS             complement(31635..32786)
                     /locus_tag="KKO08_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32851..33505
                     /locus_tag="KKO08_001879"
                     /pseudo
     CDS             32851..33505
                     /locus_tag="KKO08_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8406 a         8816 c         8267 g         8057 t
ORIGIN      
        1 aaaaacggga aaagtaatgg taaagccaca gctaaataca tagaatcccc agcacatcca
       61 taagtcagct atttactatg ctcgaattgc tttacaccgc ccttctctac cttattcagc
      121 cgctgatctg gatacggctc tgggtgcgcg gacgtaaggc tccggcctat cgaaaacgct
      181 ggggtgaacg ttacggtttt taccgccatc cgctaaaacc aggcggcatt atgctgcact
      241 ccgtctccgt cggtgaaact ctggcggcga tcccgttggt gcgcgcgctg cgtcatcgtt
      301 atcctgattt accgattacc gttacaacca tgacgccaac cggttcggag cgcgtacaat
      361 cggctttcgg gaaggatgtt cagcacgttt atctgccgta tgacctgccc gatgcgctca
      421 accgtttcct gaataaagtc gaccccaaac tggtgttgat tatggaaacc gaactatggc
      481 ctaacctgat tgcggcgcta cataaacgta aaattccgct ggtgatcgct aacgcgcgac
      541 tctctgcccg ctcggccgca ggttatgcca aactgggtaa attcgtccgc cgcttgctgc
      601 gtcgtattac gctgattgct gcgcaaaatg aagaagatgg tgcacgtttt gtggcgctgg
      661 gcgcaaaaaa taaccaggta accgttaccg gtagcctgaa attcgatatt tctgtaactc
      721 cgcagttggc tgctaaagcc gtgacgctac gccgccaatg ggcaccacac cgcccggtat
      781 ggattgccac cagcactcat gaaggtgaag agagcgtggt cattgccgca catcaggcat
      841 tgttacagca attcccgaat ttattgctca tcctggtacc ccgtcatcca gaacgcttcc
      901 cggatgcgat taaccttgtc cgccaggctg gactaagcta tatcacacgc tcttcagggg
      961 aagtcccctc caccagcacg caggttgtgg ttggcgatac gatgggcgag ttgatgttac
     1021 tgtacggcat tgccgatctc gcctttgttg gcggttcact ggttgaacgt ggtgggcata
     1081 atccgctgga agctgccgca cacgctattc cggtattgat ggggccgcat acttttaact
     1141 ttaaagacat ttgcgcgcgg ctggagcagg caagcgggct tattaccgtt accgatgcca
     1201 ctacgcttgc aaaagaggtt tcctctttac tcaccgacgc cgtttaccgt agtttctatg
     1261 gccgtcatgc cgttgaagta ctgtatcaaa accagggcgc gctacagcgt ctgcttcaac
     1321 tgctggaacc ttacctgcca ccgaaaacgc attgaggttg ttatgcaaaa acgggcgatt
     1381 tatccgggta ctttcgatcc cattaccaat ggtcatatcg atatcgtgac gcgcgccacg
     1441 cagatgttcg atcacgttat tctggcgatt gccgccagcc ccagtaaaaa accgatgttt
     1501 accctggaag agcgtgtggc actggcacag caggcaaccg cgcatctggg gaacgtggaa
     1561 gtggtcgggt ttagtgattt gatggcgaac ttcgcccgta atcaacacgc tacggtgctg
     1621 attcgtggcc tgcgtgcggt ggcggatttt gaatatgaaa tgcagctggc gcatatgaat
     1681 cgccacttaa tgccggaact ggaaagtgtg tttctgatgc cgtcgaaaga gtggtcgttt
     1741 atctcttcat cgttggtgaa agaggtggcg cgccatcagg gcgatgtcac ccatttcctg
     1801 ccggagaatg tccatcaggc gctgatggca aaattagcgt agcgtttatg ccggatggta
     1861 tgccatccgg ctcgcatgaa ttacttctgg cactgccgac aataaaacgt tgcccgctgc
     1921 gcatgtttag tcgccacaat cggcgtaccg cacacccgac acggctcacc ttttcgcccg
     1981 taaacctgca attcctgcgc gaaatagccc ggtttaccat cactttgcag aaaatctttc
     2041 agcgttgtac caccctgctc aatcgaacgc agcaacaccg ctttaatcac ccgagctaac
     2101 aattcacact ctgccagcga cagtgatgac gccagccgat ccggatggat ccccgccgca
     2161 aacagtgatt cgctggcata gatattccct acccctacca ccagcttgtt atccatcagc
     2221 cacggtttaa tcgccgtttt tttcttcgcg cacttctgat gcagatactc accattgaaa
     2281 tcgtcgctaa gcggctccgg tccaagatgg gccagcacat tatgcccttc cagctctttg
     2341 gtccacagcc aggcaccaaa gcggcgcgga tcggtgtagc gcagcacttt accgttgctc
     2401 atcaccaaat ccacatggtc atgcttttca gggggaagtt cttctggaag gatgcgcagg
     2461 ctgccagaca tccccaaatg aatgataatc cagccctcag gcagctccag cagcagatat
     2521 ttagcccgcc gctgcacgct aagcactggt tgatcgctta atcggtagat ctcttctgaa
     2581 accggccagc gcaagcgtcc gttgcgcacc accgcatgaa gaatggttgc accaacgaga
     2641 tgcggttcta tgccgcgacg gctggtttca acttcgggta attcaggcat agcatctcca
     2701 ggaatgaaca gatgcaatca atatgggggc aagcagataa caaaaaaccc cgccggagcg
     2761 aggtttttcg ttacatcaaa gcgagaatta tttgattttc gcttctttgt agatcacgtg
     2821 ctgacgaaca actggatcga attttttcag ttccagtttt tccggcttag tacgtttgtt
     2881 cttcgtagtg gtatagaagt gaccagtacc agcagaagaa accagcttga ttttctcacg
     2941 aataccttta gccatgattt atttcctcta agtacttagt acttttcgcc acgggcacgc
     3001 agttcagcca gaactgtatc gatgcctttt ttatcgatta cacgcatacc tttagcagat
     3061 acgcgcaggg tgacaaaacg cttctcgctc tcaacccaga aacggtgaga gtgcaggttc
     3121 ggcaggaaac ggcgtttagt cgcgttcagt gcgtgggaac ggttgttacc ggtcaccgga
     3181 cgcttgccag taacttggca gactcgggac atgtctattc tccaaaaatc aaattagctc
     3241 gagcttcgta tggggtattg gcgcctcgtc aggctttaca gcccggtcat cgcagttcta
     3301 tgtgaactct cgattgccag gcccaaatgc caaacccgag attctcaaag gtggcgtagt
     3361 atacgctgac tcagcgatgt gctcaagtcc cgaacagaca aagatcccga aggatcgcgc
     3421 atagcgggtt aaatccagcc gcgttcggca aaagaaacat actctccacg cccaatcacg
     3481 atatggtcga gcacgcgtaa atccatgaac tgacaactct ttattatccg ttcagtaatg
     3541 agtttatccg ctttactggg ttcagcacaa cccgaagggt gattatgtgc aaggatcagc
     3601 gccgaggcgt ttatttttat cgcttcgcgg ataatttccc gaggatggac ttcaacatgg
     3661 tttagcgtgc cggaaaaaag acggctatgc gttataaccc ggtgttgtga gtcgagaaag
     3721 atcaccataa agatctcccg ctcctcaccc gtgagctggc tttgtaaaaa ttcccgcgtc
     3781 atctccgggc tgagtaaagg gctttcttca cgcatccgca cgttgtagta acgtcgcgcc
     3841 agttcagcaa tcccctttaa ctgggcaaat ttcgctacgc caattccatg aacaccacta
     3901 aattgttcat attcagaggt taacaagcca taaagagagc cgaaattctc cagcatctct
     3961 tttgccaggg ttaatacatc tttaccgcgc gttccggtac gcagaaatag cgccagcagc
     4021 tcgacatccg ttaaggcgct aataccaaac ttcagcattt tttcgcgcgg catcaacagc
     4081 tgtgcattgt ttttcacctt cacctccttt gtggtgctcg catcctgtca tagccgttcc
     4141 gttaaatcga cggccagttt tcaatcctgg aaagagcctc gcaaagtgaa tctcaggtga
     4201 aggcacgaca acaaaagtat tgtgataaaa tcgccaactt ctggtgtcac ccaacaggaa
     4261 aaatcatcat gagcctggcc ggtaaaaaaa tcgttctcgg cgttagcggc ggtattgctg
     4321 cctataaaac ccctgaactg gtgcgtcgtt tgcgcgatcg cggggccgac gtccgcgtag
     4381 ccatgaccga agcggcaaaa gcctttatca ccccacttag cttgcaggcg gtttctggtt
     4441 atcccgtttc cgacagtctg cttgacccgg cagccgaagc cgctatgggc catattgagc
     4501 tgggtaaatg ggctgattta gtgattctcg cccctgccac ggcagatttg attgcccgtg
     4561 ttgctgccgg aatggcgaat gacctggtat cgacgatttg tctggctaca cctgcgcctg
     4621 tagccgtgct ccccgccatg aaccagcaga tgtaccgtgc cgctgccacg cagcataatt
     4681 tagaggtgct tgcttcccgt ggtttgctca tctgggggcc agacagtggc agtcaggctt
     4741 gtggtgatat cggtcctgga cgaatgctcg atccgttaac cattgtggat atggcggtgg
     4801 cgcatttttc gcccgtcaac gacctgaaac atctgaacat tatgattacc gccggtccga
     4861 cgcgtgaacc gctcgatccg gtgcgttata tctctaatca cagctccggc aagatgggtt
     4921 ttgctatcgc cgccgctgct gcccgtcgtg gcgcgaacgt cacgctggta tcaggtccag
     4981 tttcactacc gacgccaccg tttgttaaac gtgttgatgt gatgaccgcg ctggaaatgg
     5041 aagccgccgt gaatgcttct gtacagcagc aaaatatttt tatcggttgc gccgccgtgg
     5101 cggattatcg cgcagctacc gtggccccag aaaaaatcaa aaagcaggcc acgcagggtg
     5161 atgaattaac aataaaaatg gttaaaaacc ccgatatcgt cgcaggcgtt gccgcactaa
     5221 aagaccatcg accctacgtc gttggatttg ccgccgaaac aaataatgtg gaagaatacg
     5281 cccggcaaaa acgtatccgt aaaaaccttg atctgatctg cgcgaacgat gtttcccagc
     5341 caactcaagg atttaacagc gacaacaacg cattacacct tttctggcag gacggagata
     5401 aagtcttacc gcttgagcgc aaagagctcc ttggccaatt attactcgac gagatcgtga
     5461 cccgttatga tgaaaaaaat cgacgttaag attctggacc cgcgcgttag gaaggaattt
     5521 ccgctcccga cttatgccac ctctggctct gccggacttg acctgcgtgc ctgtctcgac
     5581 gacgccgtag aactggctcc gggtgacact acgctggttc cgaccgggct ggcgattcat
     5641 attgccgatc cttcactggc ggcaatgatg ctgccgcgct ccggattggg acataagcac
     5701 ggtatcgtgc ttggtaacct ggtaggattg atcgattccg actatcaggg ccagttgatg
     5761 atttccgtgt ggaaccgtgg tcaggacagc ttcaccattc aacctggcga acgcatcgcc
     5821 cagatgattt ttgttccggt agtacaggct gaatttaatc tggtggaaga tttcgacgcc
     5881 accgaccgcg gtgaaggcgg ctttggtcac tctggtcgtc agtaacacat acgcatccga
     5941 ataacgtcat aacatagccg caaacatttc gtttgcggtc atagcgtggg tgccgcctgg
     6001 caagtgctta ttttcagggg tattttgtaa catggcagaa aaacaaactg cgaaaaggaa
     6061 ccgtcgcgag gaaatacttc agtctctggc gctgatgctg gaatccagcg atggaagcca
     6121 acgtatcacg acggcaaaac tggccgcctc tgtcggcgtt tccgaagcgg cactgtatcg
     6181 ccacttcccc agtaagaccc gcatgttcga tagcctgatt gagtttatcg aagatagcct
     6241 gattactcgc atcaacctga ttctgaaaga tgagaaagac accacagcgc gcctgcgtct
     6301 gattgtgttg ctgcttctcg gttttggtga gcgtaatcct ggcctgaccc gcatcctcac
     6361 tggtcatgcg ctaatgtttg aacaggatcg cctgcaaggg cgcatcaacc agctgttcga
     6421 gcgtattgaa gcgcagctgc gccaggtatt gcgtgaaaag agaatgcgtg agggtgaagg
     6481 ttacaccacc gatgaaaccc tgctggcaag ccagatcctg gccttctgtg aaggtatgct
     6541 gtcacgtttt gtccgcagcg aatttaaata ccgcccgacg gatgattttg acgcccgctg
     6601 gccgctaatt gccgcgcagt tgcagtaata tgacgccgga tgacttttca tccggcgagt
     6661 ttctttaaac gccaaactct tcgcgatagg ccttaaccgc cgccagatgt tccgccattt
     6721 ccggcttctc ttccagatag gcaatcagat ctttcagggt gatgatagag atcactttgc
     6781 agttgtaatc acgctcaact tcctgaatcg ccgaaatctc gccgcgcccg cgttcctgac
     6841 gatcgagcga aatcaacacg ccagcaagcg tcgcgccatt ggcctgaata atctccatcg
     6901 actcgcgaat cgccgttccg gcggtgatca catcatctac cagcattacg cgtccttgta
     6961 acgcgctacc aaccagattg ccgccttcac cgtggtcttt tgcttctttg cggttaaagc
     7021 agtacggcag gtccaggtcg tgatgctccg ccagtgccac agcggttgtg gtggcaatcg
     7081 ggatcccttt gtaagcaggg ccaaacagca gatcgaactc aatgccggaa tccaccaacg
     7141 cttcagcgta aaaacggcct aacagtgcca gatcgcgccc ggtattaaac agcccggcgt
     7201 tgaagaaata ggggcttttg cgcccggatt tcagcgtaaa ctcgccaaac tttaacacct
     7261 gcttgctaag cgcaaattca ataaactggc gctgatatgg tttcatgcct tcgctcctca
     7321 tcttactttt ctacagacaa aaaaaaggcg actcatcagt cgccttaaaa atcagtttgc
     7381 cagcgccgcc ttctgcgtcg ctacaatgga ttcgattccc cctcgggcca gcgccaacaa
     7441 ggtgagtagc tcttcatggg tgaacggctc gccttctgcc gtcccctgca cttcaatgat
     7501 gcgcccgtct tcggtcatca ctacgttcat gtcggtctct gcggctgagt cttcaacgta
     7561 ttccagatcg caaattgctt caccgtttac aataccgaca gaaactgcgg ctaccatccc
     7621 tttcatcggg ttggttttca gcttgccgtt ttccaccagc ttctgtagcg catctgccag
     7681 cgccacgcag gcacccgtaa tcgacgcggt acgagtgcca ccatcagcct gaagcacgtc
     7741 gcagtccagc gtaatggtga actcacccag cgctttcaaa tctactgccg cgcgaagagc
     7801 acgggcgatc agacgctgga tttccattgt gcgtccaccc tgcttacctt tcgccgcttc
     7861 acgagcgtta cgggtgtggg tagaacgtgg cagcatgccg tactctgcgg tgatccagcc
     7921 ctggccctga cctttcagga agcgcggcac gccttcttca atagaggcgg tacacaatac
     7981 tttggtatcg ccaaattcga ccagcaccga gccttctgca tgttttgtat agttacgagt
     8041 cagggtaacg ggacgcacct gattattgct acggcctgct ggacgcatat tgaaatctcc
     8101 ggcttgaaac aaatgtggct gcgcattata cggacttccg gcggttattc ctatcctgac
     8161 aaggcatcga tggctataat ccttccacct ctccttttat aaacaggaac gtctatgatc
     8221 cgcagtatga ccgcctacgc ccggcgtgaa atcaagggtg aatgggggag cgcaacctgg
     8281 gaaatgcgct cggtaaacca gcgttatctg gaaacttact ttcgtctgcc ggagcagttc
     8341 cgtagccttg aacctgtcgt tcgcgagcgt attcgttctc gcctgacgcg cggtaaagtg
     8401 gaatgtaccc tgcgctatga gccagatgtt agcgcacaag gtgagctgat cctcaatgaa
     8461 aaactggcta aacagctggt aactgccgcg aactgggtaa aaatgaagag tgacgaaggg
     8521 gaaatcaacc cggttgatat tctacgctgg ccgggcgtga tggcagccca ggagcaggat
     8581 ctcgacgcga ttgccgctga aattctcgcg gcgttggatg gtacgctgga cgactttatc
     8641 gtcgcgcgcg aaaccgaagg tcaggcactg aaagcattga tcgagcagcg tctggaaggc
     8701 gtcaccgccg aagtggtcaa agtccgcgcc catatgccgg aaattctgca atggcagcgt
     8761 gagcgtctgg tcgcgaagct ggaagatgct caggttcagt tggaaaacaa ccgtctggag
     8821 caggagttgg ttctgctggc acaacgaatt gacgttgccg aagagctgga ccgcctcgaa
     8881 gcgcatgtta aagagacgta caacattctg aagaaaaaag aagcggttgg tcgtcgtctg
     8941 gactttatga tgcaggagtt caaccgtgag tcgaacactc ttgcgtcgaa atctatcaat
     9001 gccgaagtga caaactccgc catcgagctg aaagtgttga ttgagcagat gcgcgagcag
     9061 attcagaata ttgagtaact cgtaacacca attctgactt ttcagccttc acaaatgccg
     9121 cccactcaaa cagagcggca tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg
     9181 ctccagattt tgcgatgttt ttttgcccag cacactgaga acgtgagata ctcacaactg
     9241 tatataaata cagttacaga tttactttct ttgcaatcga tatcacatgg agtgggcaat
     9301 gaacgaacat catcaacctt ttgaagagat aaaactgatt aatgcaaacg gagcagaaca
     9361 atggtcagca agacaacttg ggaaactact gggttattca gagtatcgtc actttatacc
     9421 tgtattaacg cgcgccaaag aggcctgtga gaacagtggt cacacaattg atgaccattt
     9481 cgaggagatc ctcgatatgg tcaaaattgg ctcaaatgcc aaaagagcat taaaagacat
     9541 cgtactctcc cgctatgcct gttacctggt agtacaaaac ggcgaccccg cgaaaccggt
     9601 cattgcggca gggcagactt attttactat ccagacccga cggcaggagc ttgctgacga
     9661 tgaagcattt aagcaacttc gtgaagacga aaagcgtctg tttctgagaa atgagttgaa
     9721 agaacataac aaacaattgg ttgaggcagc acagcaagct ggggtagcga cagctactga
     9781 tttcgccata tttcagaatc atggttacca ggggctgtat ggtggattag atcagaaagc
     9841 catccaccaa ctgaaggggc tgaaaaagag tcagaagatc ctggatcata tgggttcaac
     9901 agaactggcg gctaatctct ttcgagctac ccaaacagaa gaaaaactca agcgggatag
     9961 cgtgaattca aaacagcaag ctaataccac acactttgac gtgggtagca aggtgaggca
    10021 aaccattcag gaacttggcg gaaccatacc tgaggagttg ccgaccccgc aggtcagcat
    10081 caagcagttg gaaaacagtg taaaaattac agagaagaaa tagcagaaac gaaaaactct
    10141 ttttgttgat tgagacaccc gatgcgtaag ccaaggtcca ggtgcagtga acacaatggc
    10201 taaacattgc acctttcttt tcccccctca gttttaacct attttttctt atgcattttc
    10261 tcagacaaga agtcagaaga atgcatctac gctacagaaa atagcgattt cacataacta
    10321 aaagttatca atttcctctc accttaaaaa atatcaatcg tgacaatgcg cacaaatcgc
    10381 taccctgcca gacagatttt tagggagaga accatgctgt tacacatttt gtatttggtt
    10441 ggcattactg ccgaagccat gacgggggcg ctggcggccg ggcgacggcg catggataca
    10501 tttggcgtaa ttattattgc aaccgccacc gcaattggcg gagggtcagt gcgcgatatt
    10561 ctgctgggcc actatccgct cggctgggtc aaacacccgg aatatgtgat tatcgtcgct
    10621 accgccgcag tactcaccac tatcgttgct cctgtaatgc cttacctgcg caaagtgttt
    10681 ctggtgctcg atgcgctcgg actggtggtc ttttctatca tcggcgcaca ggttgcactg
    10741 gatatggggc acggcccaat tattgccgtt gtcgcggcgg taaccaccgg tgtatttggc
    10801 ggcgttttgc gcgatatgtt ctgtaaacgc attccgctgg tctttcaaaa agagttgtat
    10861 gccggtgtct catttgcctc cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac
    10921 catgatgtgg tgattatctc caccctggta ttcggctttt ttgctcgttt actggcgcta
    10981 cgtcttaagc tgggattacc ggtcttttac tacagccacg aaggtcacta aggttcaaaa
    11041 cctgtgatct gctgggcagc cagccaactg cccagcttct tgatttgcgc attttccttc
    11101 cattcaataa cctgtctggc gcgcccagat ccagtccccg gcagctgctg ccagaactgc
    11161 tccgtgctaa ataaaagttg cgaccaggac cgttcatcac tggcattaag cgccgcccgg
    11221 gttagcggta ttcccattgc catcacccag cgagtaaaag gctggttacg agccagatta
    11281 aactgatgcc ataactgcgc acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc
    11341 tctggcgtta ataaaagcca ggaaaagata tgctcaaagc gatgagtctg atgcagcgcg
    11401 cgccaaccgg cctcaccaat gccatccagc cccagaacct gttttgaccc cagccagact
    11461 aagcgtgaaa tgaactgttc ctgacaaaca tcagaagcaa agtagcaggt caacgggtta
    11521 aagcggtttt ctggtggtgt cggttttgta cgttctgcac cgcgccagac cacatcatca
    11581 atgcgaggaa taccctgacc ggcaaggctg acgaggatct gatcaccagg cgcaatatcc
    11641 cactcctgcc agcgcctgac ggaaccaata ttcacccgct ggactttttt atcatccagc
    11701 atgacaggtg cgagtgacgc aaccaccgat attttaccgc tcttacccac cgcaaactga
    11761 atcgccttca cttcggcaac ctgagctaca ggttgatatt tccaggccac cagccactcc
    11821 gcctggcccg gtagccaatg acgggattcg ggttccttag cccctcgcac aaccacgcca
    11881 tcggtgacga agggtaattt cgctttccac cactcattgc gtacgcgcgc aacctcatca
    11941 gcatttttaa ccgcacgggt atacctctgc gttagagtaa aacctgcggt agccagctcc
    12001 tttaaacgat cggtcattaa ctgcggtcca tccggccatg cccagacaaa aacagccagt
    12061 gagtttagcg tgtcgctatt cccctggcgc atcatcaggc cagcaacttt tgcgcgggca
    12121 tttattcccc ccatttgttg ttggatatgc ccctcgcgct ggagaaatat ttccccctga
    12181 agcgtactgt tggctaaagg cccgctaacg gtttgcggca cagcggaaat taagctaact
    12241 ttctgcgtcc agtcctcgcc tttcaggcca ttaccgcgac tgattgcttt gttcagtttc
    12301 ccgtcccgat aaaccagggt taccgcaacg ccatcaactt ttggctgcac ccaaagatcg
    12361 ctacgttctc gcatccacag acttaatgcg tttttatccg ccattttgcg tacacccgta
    12421 tgggcaaccg gatgcataac cgcaccattt aaaggcggca tcatcacgtc gcggggctcg
    12481 ctcacaaagc agcgttgcca ctgcgtaaga cgggcgctta actgatcgta aacaccgtct
    12541 tccacctcac tttttccttc cttccagtag tcatcgtccc actgttttat ttgctgttgc
    12601 aggcgggaaa tttcttcctg tgctctggct ggcgaccagg ccggacagac cgcccacaca
    12661 gatgattgcc agcacaagat acttattaat atcgccatcc atactttcat cttcacctcc
    12721 gctgtagtca ggctggcaga tatacaacgt ggtgaaggca aagccgagtg gcaaaaacgg
    12781 agtctgcgag gacgcttcct gagaatcgtc tttattgcag taaatcacag gcaaatgcgg
    12841 aaacagctac gcaaaacgca acaactttgc gcaaaaagtg tgagcaaggg ctacgtcaca
    12901 tggccgcgcc gtgtataata agctcgtatg taggctttat ttcgctaatc acatacgaaa
    12961 gatactcatg gctcaaggca cgctttatat tgtttctgcc cccagtggcg cgggtaaatc
    13021 cagcctgatt caggctttat taaaaaccca accgttgtat gacacccagg tttctgtttc
    13081 acacaccaca cgccaaccgc gtcctggtga agtccacggt gaacattatt tctttgttaa
    13141 tcatgatgaa tttaaagaaa tgattagcag agatgcgttc ctcgaacacg cagaagtttt
    13201 tggtaattac tatggcactt cgcgtgaggc cattgagcaa gtactggcga ccggtgtcga
    13261 tgtttttctc gatatcgact ggcagggcgc gcagcaaatt cgccagaaga tgccgcacgc
    13321 gcggagtatc tttattttac cgccgtccaa aattgaactg gaccgccgtc tacgcggtcg
    13381 cggtcaggac agcgaagagg tcattgcaaa gcgtatggcg caagctgttg cagaaatgag
    13441 ccattacgcc gaatatgatt atctgattgt gaatgatgac ttcgataccg cgttgaccga
    13501 tttgaagacc attattcgcg ccgaacgtct gcgcatgagc cgccaaaagc agcgtcatga
    13561 cgctttaatc agcaaattgt tggcagactg aacctgattt cagtatcatg cccagtcatt
    13621 tcttcacctg tggagctttt taagtatggc acgcgtaact gttcaggacg ctgtagagaa
    13681 aattggtaac cgttttgacc tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt
    13741 aggcggaaag gatccgctgg taccggaaga aaacgataaa accactgtaa tcgcgctgcg
    13801 cgaaatcgaa gaaggtctga tcaacaacca gatcctcgac gttcgcgaac gccaggaaca
    13861 gcaagagctg gaagccgctg aattacaagc cgttaccgct attgctgaag gtcgtcgtta
    13921 atcacaaagc gggtcgccct tgtatctgtt tgaaagcctg aatcaactga ttcaaaccta
    13981 cctgccggaa gaccaaatca agcgtctgcg gcaggcgtat ctcgttgcac gtgatgctca
    14041 cgaggggcaa acacgttcaa gcggtgaacc ctatatcacg cacccggtag cggttgcctg
    14101 cattctggcc gagatgaaac tcgactatga aacgctgatg gcggcgctgc tgcatgacgt
    14161 gattgaagat actcccgcca cctaccagga tatggaacag ctttttggta aaagcgtcgc
    14221 cgagctggta gagggggtgt cgaaacttga taaactcaag ttccgcgata agaaagaggc
    14281 gcaggccgaa aactttcgca agatgattat ggcgatggtg caggatatcc gcgtcatcct
    14341 catcaaactt gccgaccgta cccacaacat gcgcacgctg ggctcacttc gcccggacaa
    14401 acgtcgccgc atcgcccgtg aaactctcga aatttacagc ccgctggcgc accgtttagg
    14461 tatccaccac attaaaaccg aactcgaaga gctgggtttt gaggcgctgt atcccaaccg
    14521 ttatcgcgta atcaaagaag tggtgaaagc cgcgcgcggc aaccgtaaag agatgatcca
    14581 aaaaatcctc tcggaaatcg aagggcgttt gcaggaagcg ggaattccct gccgcgtcag
    14641 tggtcgcgag aagcatcttt attcgattta ctgcaaaatg gtgctcaaag agcagcgttt
    14701 tcactcaatc atggacatct acgctttccg cgtgatcgtc aatgattccg acacctgtta
    14761 tcgcgtgctc gggcaaatgc atagcctgta caagccgcgt ccgggtcgcg tgaaagacta
    14821 catcgccatt ccaaaagcga acggctatca gtctttgcac acctcgatga tcggcccgca
    14881 cggtgtgccg gttgaggtcc agatccgtac cgaagatatg gatcagatgg cggagatggg
    14941 tgttgccgcg cactgggctt ataaagagca cggcgaaacc agcaccaccg cgcaaatccg
    15001 cgcccagcgc tggatgcaaa gcctgctgga gctgcaacag agcgccggta gttcgtttga
    15061 atttatcgag agcgttaaat ccgatctctt cccggatgag atttacgttt tcacaccgga
    15121 agggcgaatt gtcgagctgc ctgccggtgc aacgcccgtc gacttcgctt atgcagtgca
    15181 taccgatatc ggtcatgcct gcgtgggcgc acgcgttgac cgccagcctt acccgctgtc
    15241 gcagccgctt accagcggtc aaaccgttga aatcattacc gcaccgggcg ctcgcccgaa
    15301 tgccgcgtgg ctgaactttg tcgttagctc aaaagcgcgc gccaaaattc gtcagttgct
    15361 gaaaaacctc aagcgtgatg attctgtaag cctgggccgt cgtctgctca accatgcttt
    15421 gggtggtagc cgtaagctga atgaaatccc gcaggaaaat attcagcgcg agctggatcg
    15481 catgaagctg gcaacgcttg acgatctgct ggcagaaatc ggacttggta acgcaatgag
    15541 cgtggtggtc gcgaaaaatc tgcaacatgg ggacgcctcc attccaccgg caacccaaag
    15601 ccacggacat ctgcccatta aaggtgccga tggcgtgctg atcacctttg cgaaatgctg
    15661 ccgccctatt cctggcgacc cgattatcgc ccacgtcagc cccggtaaag gtctggtgat
    15721 ccaccatgaa tcctgccgta atatccgtgg ctaccagaaa gagccagaga agtttatggc
    15781 tgtggaatgg gataaagaga cggcgcagga gttcatcacc gaaatcaagg tggagatgtt
    15841 caatcatcag ggcgcgctgg caaacctgac ggcggcaatt aacaccacga cttcgaatat
    15901 tcaaagtttg aatacggaag agaaagatgg tcgcgtctac agcgccttta ttcgtctgac
    15961 cgctcgtgac cgtgtgcatc tggcgaatat catgcgcaaa atccgcgtga tgccagacgt
    16021 gattaaagtc acccgaaacc gaaattaatg ttttatgaac ccaacacgtt atgcacgcat
    16081 ctgcgaaatg ctcgccaggc ggcagcctga tctgaccgtc tgcatggagc aggtccacaa
    16141 acctcataac gtttctgcga ttattcgtac cgcagatgcc gttggcgtac atgaagttca
    16201 cgccgtctgg cctggtagcc gcatgcgcac catggcttcg gcagcggcgg gtagtaacag
    16261 ctgggtacag gtgaaaacac accgcaccat tggcgatgcc gtcgctcatc tcaaaggcca
    16321 gggcatgcag attctggcaa cccatctttc tgataatgct gtcgatttcc gcgaaattga
    16381 ttacactcgc ccgacctgca ttttgatggg acaggagaaa acgggcatca cgcaggaagc
    16441 attggccctg gcggatcagg acatcatcat tccgatgatc ggcatggtgc agtcgctgaa
    16501 tgtttccgtt gcctcagccc tcattcttta cgaagcccag cgtcagcggc aaaatgcagg
    16561 catgtacctg cgtgaaaaca gcatgttgcc ggaagcagag caacaacgcc tgttgtttga
    16621 aggcggctat ccggtgctgg cgaaagtcgc aaaacgcaaa ggcctgcctt atccccacgt
    16681 caatcagcaa ggcgagatcg aagctgatgc cgactggtgg gctactatgc aggctgcagg
    16741 gtaagtgcca tgaaaggtcg cctgttagat gctgtcccac tcagttccct aacgggcgtt
    16801 ggcgcagcac ttagtaacaa actggcgaaa atcaacctgc ataccgtgca ggatctactc
    16861 ttacaccttc ccctgcgcta cgaagatcgc acccatctct accccatcgg agaactactg
    16921 ccgggcgttt atgccacggt ggaaggcgaa gtgctgaact gcaatatctc cttcggcggt
    16981 cggcggatga tgacctgcca gatcagcgac ggttccggca tcctcaccat gcgctttttc
    17041 aatttcagcg cggcaatgaa aaatagcctg gcgacgggcc gccgtgtact ggcttatggc
    17101 gaagcaaagc gcggtaaata tggtgcggag atgatccacc cggaataccg cgtgcagggc
    17161 gatctcagca cgccagaatt acaggaaacg ctcacgccgg tttatccaac aacggaaggc
    17221 gtaaagcagg ccacgctgcg taaattaacc gaccaggcgc tggatctgct cgacacctgc
    17281 gccattgaag aactcctgcc gccggaactg tcacaaggaa tgatgacgct accggaagcg
    17341 ttgcgcactt tgcaccgccc gccaccgacg ctacagctta gcgatctgga aaccgggcag
    17401 catccggcgc aacgtcgtct gattctggaa gaactgctgg cgcacaacct cagcatgtta
    17461 gccttacgtg ccggagcaca gcgttttcat gcccagccgc tgagcgccaa tgacacgctg
    17521 aaaaataaac tcctcgccgc cttaccgttc aagccaacgg gcgcacaggc acgcgtagtg
    17581 gcggagatcg agcatgatat ggcgctggat gtgccgatga tgcgcctggt acagggcgat
    17641 gtaggttccg gtaaaacgct ggtcgccgcc ctcgccgcgc tgcgtgcgat tgcccacggc
    17701 aaacaggtgg cactgatggc accaaccgaa ttactcgccg agcagcacgc caataacttc
    17761 cgcaactggt ttgcaccgct cggtatcgaa gtgggctggc tcgccggtaa gcagaaaggc
    17821 aaagcgcgac tgtctcagca ggaagccatc gccagcggtc aggtgcagat gattgtcggt
    17881 acacacgcca tcttccagga acaggtgcag tttaacggcc tggcgctggt gattatcgac
    17941 gaacagcatc gttttggcgt gcatcagcgt ctggcattgt gggagaaagg ccagcagcag
    18001 ggcttccatc cgcatcagtt gatcatgacc gccacgccga tcccccgcac gctggcaatg
    18061 acggcgtatg ccgatctcga tacctcggtg atagatgagc tgccgccagg ccgcacgcca
    18121 gtgactacgg tcgctattcc tgatacccgc cgtaccgaca tcattgaccg cgtgcgccac
    18181 gcctgcataa ctgaaggtcg tcaggcgtac tgggtttgta cgttgattga agagtcagag
    18241 ttactggaag cgcaggcggc ggaagctacc tgggaagagt tgaaactggc gctaccagaa
    18301 ttgaatgttg gcctggtaca cgggcggatg aaaccagccg agaaacaggc ggtgatggcg
    18361 tcgtttaaac aaggtgagct acatctgctg gttgccacta ccgttattga agtcggtgtt
    18421 gatgtgccta acgccagcct gatgattatc gaaaaccctg agcgtctggg tctggcgcag
    18481 ttacaccagc tgcgcgggcg cgtaggtcgt ggcgcggtgg cttctcactg cgtgctgctc
    18541 tacaaaacgc cgctttctaa aacggcgcaa attcgcctgc aagtgctgcg cgacagtaac
    18601 gacggttttg tgattgcgca aaaagatctg gagattcgcg gccctggcga attgttaggc
    18661 acgcgtcaga cgggtaatgc tgaatttaaa gtggcggatt tactgcgcga tcaggcgatg
    18721 atcccggaag ttcagcgcct ggcgcgacat attcacgaac gttacccaca acaggcaaaa
    18781 gccttgatag aacgctggat gccggagaca gaacgttact cgaatgcgta aaaggcggcg
    18841 gtgagaagac cgccgcttca tcggttaacc ggcaaaaatc ggcaacatta aatacagctt
    18901 aatcaccagc gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa
    18961 cgccatgtgc gacgggccaa agcgttcagt gatcgcctgc atgttggcga ttgccgttgg
    19021 cgttgcaccg aggccaaaac cacagtgacc cgcagccagc actgccgcat cgtagttttt
    19081 gcccatcatg cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac
    19141 cagaatcgcc agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa
    19201 cgccatcgcc aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa
    19261 gacgcggtaa aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac
    19321 acagacgaag gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc
    19381 caccgtcagg cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat
    19441 gcgtcccaca tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt
    19501 cggcgtggtg gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag
    19561 caccagaccg aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg
    19621 ttcaatgaac aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga
    19681 accggccaac agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat
    19741 ggcattttgc atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc
    19801 accggcacgc aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat
    19861 taacggatcg cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac
    19921 tagtagcgcc agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt
    19981 caaaaaggag acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac
    20041 aagcgttgct aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt
    20101 catactcgtc atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt
    20161 atgctttcct gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga
    20221 aaaataccaa aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat
    20281 gtggcaaacg tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga
    20341 ttgttttcga tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgctcagact
    20401 caaaacagcg aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt
    20461 gccgcctgtc agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc
    20521 tgccaggcgc tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt
    20581 gcctccggtg tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg
    20641 ttgtctattc agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg
    20701 ctgaaaaccg gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg
    20761 ctggcaagtt gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt
    20821 acgccgctgg tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg
    20881 ctgacctcca ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa
    20941 aatctgctgc tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac
    21001 ccttacttac gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg
    21061 tttatgggca tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca
    21121 acgccgctct attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc
    21181 tttatgatca cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa
    21241 cagccagtgt ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc
    21301 ctgaactcgt ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac
    21361 aacggagtga tccagttgac tggtgttgcc agccgctatg tcggttttgt cgtcgcgctg
    21421 atgttgatcg tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa
    21481 ccggttctgg gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt
    21541 atcgtttctc gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg
    21601 gttggtctgg gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa
    21661 aacctgctct cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt
    21721 ttcccaccag aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca
    21781 cctctttcac cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg
    21841 catttcacgg gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc
    21901 gctctgttag tggtgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa
    21961 catatcagcg catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggat
    22021 caccgttttt ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggt
    22081 cagcccgcgc ccctggtggc aaaaagtgtc gacattgcgc taagcagtcg ccaactgacc
    22141 gaaccacgcc atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag
    22201 accgcgccgc taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc
    22261 ccgaatagcg aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca
    22321 ccagaagccg gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt
    22381 tcgctcaatg atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc
    22441 gttacgctga ctaacctggg tgccgacatc gcccgcggga cattaaccgg aaacgcgcag
    22501 cgtaacgccg acggcagctg gcaagtggaa aatctgcgca tggcggatat ccgtctacaa
    22561 agcgaaaaat cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt
    22621 ggtcgcttgg aagtcatcga tgctcgtctg caaggtccgg actgggcggt gaccgacctc
    22681 gatctcagct tgcgcaacat gaccttcagt aaagatgact ggcagacaca ggaaggcaaa
    22741 ctgtcgatga acgccagcga gttcatttat ggttcgctgc atttatttga cccgattata
    22801 aacgcggaat tttccccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg
    22861 ggtatggtca gaacgtcagg gaactggctg cgtgacggta aaacgttgat ccttgatgat
    22921 gcggcaattg ccgggctgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa
    22981 acgacacccg gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg
    23041 atcattgata tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc
    23101 aacctgacgc tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat
    23161 gccgccgccg cgacattcaa tcgtgttgat gttcgtcgcc cgtcgctggc gctgaccgcc
    23221 aacagcagca cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa
    23281 gccaccgcaa gtgtttcaca aacgccacaa cgtcagaccc atatcagcct gaatggacgc
    23341 ggtgtgccgg tgaatatttt gcaacaatgg ggatggcctg aattaccgtt gactggcgac
    23401 ggcaatattc agcttaccgc cagtggagat attcaggcca atgccccgct gaaacctacg
    23461 gttagcgggc aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat
    23521 gcgggcgtcg tttccagcgg tgaagttaca tcgacggagc cggtgcggta aacatcaatc
    23581 tcgccgggta cgactcagac gtacccggca ttccgtcaat agattacaac gtaattgtca
    23641 gcgcattccc ttgaggcgtc accaccagcc cctgctcact ttcagcctgc gaaccgcctt
    23701 gcagaccatt tactttcaca acattgcgca agcacagcgt ccagttcttc gcctcgcccg
    23761 caccagtcac agtaatcgtg ttgccagtac gtgctgcttt taaagtaaag atcaccgatc
    23821 cgtcagcagc aggcacttca cagacggctt catgcccgtc ttgcagatta aagaggtgga
    23881 atgccgtgcc ttcgtgccac acgtaatcgg gacgttgatc gttgttgccc agcgccagta
    23941 gagtgttatc acgcacataa acgggcagac tcaggaagcc gtgctgctgt ttatgccagc
    24001 gactaccgtc gagttcatcg ttgtgccaca ggtgtgtcca gcgaccttcc ggcaggtaga
    24061 actgcacatc gcccgcttca gtgaacaccg gcgcaaccat cacgttgtcg cctaacatgt
    24121 attgacggtc aaggtaatca caagccggat cgtccgggaa ctccatcatc atggcccgca
    24181 tcatcggcgt accccgcgcg ttcgcacgcg cagcttcacg atacagatac ggcatcatgc
    24241 ggcatttcag ttgcgtgaag aagcgcacca catcacagga ctcatcatcg taggcccacg
    24301 gcacacgata agatttgcta ccgtgtaaac ggctatggct ggagagcaaa ccaaacgcgc
    24361 accagcgttt gtaaacgtgc gccggagcgg tattttcaaa gccgccgata tcgtggctcc
    24421 agaagccaaa acctgaaagg ccaatagaca aaccaccgcg caggctttcc gccattgatt
    24481 cgtagttagc gtaacaatcg ccaccccagt gtaccgggaa tttctgcgca ccgacggagg
    24541 ccgagcgggc aaacaagaca gcttcttcct caccaacggt gtccttgagc acgttccaca
    24601 ccagttcgtt gtagatgtac gcataatggt tatgcatttt ctgcggatcg gaaccgtcaa
    24661 accactgaac atcaattggg atacgttcgc caaagtcggt cttaaagcaa tcaacgccca
    24721 tcgcgaccag acctttcagt ttgtcggcgt accatttgca ggcatccgga ttggtaaagt
    24781 cataaatcgc cagacctggc tgccatttat cccactgcca tagcgaaccg tccgggcgtt
    24841 tgagtaaata gcctttctct tgtaactctt taaagacggg ggatttttga ccgatatagg
    24901 ggttaatcca gacgcagatt ttcagccctt tcgctttcag gcggcggatc atcccttccg
    24961 ggtcagggaa agtcagcggg tcccactcaa aatcgcacca ctggaaggct ttcatccaga
    25021 aacagtcaaa gtggaaaaca tgcagcggca gattgcgttc cgccatacca tcgataaagc
    25081 tgtttaccgt cgcttcgtcg tagttggtgg taaatgaagt ggttagccac aggccgaagg
    25141 accacggggg cggcagcgcc ggacgaccag taaagcgggt ataacgatca agtaccgctt
    25201 tcggcgtcgg gccgtcgata acaaagtatt cgagatattc actctcaacg ctgaactgca
    25261 ctttggagac tttctccgat cccacttcaa aagagacaca ctggggatga ttgaccagta
    25321 ccccataacc acggttagtc atgtagaacg ggatattttt atacgcctgt tcagtacttg
    25381 tgccgccgtc ccggttccag gtctctaccg tctggccatt gcgcaccagg gcagtaaagc
    25441 gctctcccag accgtaaact gtttcgccaa cgccaagatc aagccgctca aacatataat
    25501 tgcgttgatt attcgtgtcc tgcacgtagc cattattttt cacctgacta ccggtaatac
    25561 gttcgccgtt gcgcagaaaa tccagtgacc agaactcacc tttgctgaca cgcgcgctta
    25621 agttgccact tttaaactca gcataacgtt ctgtgttttc gattgtgacc ttcacgtcct
    25681 gcaaaatatt gagcggataa tgaggaccgt tattcagcgc cccctgaaaa tgctcaatcc
    25741 gcacaccgac aataccttcc tgtggggaga aaaagcgcag cgtaaataaa ggcgtatcaa
    25801 gctgccaggt acgttcacgc acatcacggg gggcagcata gaccaccatt tcattatcct
    25861 gctgttcaac ctcgaatacc tgaagcgggt gaatcaaatt gaggccaggt tgaatcaacc
    25921 agtttccatc gctaattttc attgccgttc cttagttctg cacttcttga gaggtgaatt
    25981 gttgctggca acggcgttta ccctgagcca gctgttccat aacggttttc agattgtgcg
    26041 tcgtgagtga gtagtagcgt ttagcgataa tcgcgctcag caaataacag atcgccggaa
    26101 caatcgtgaa tagcgcaata atgatgctaa tcgtggcgct gttctgcgct ttttctgccg
    26161 catcatatcc gccataagcc agcatccagc cgataagagc gccgccgaag gccaacccca
    26221 gtttgagcac aaacagcgtg ccagcaaaac tgatcccggt caggcgttta ccattgcacc
    26281 actcgccgta gtcgacggta tcgaacatca ttacccactg gataggtgtc accagttgat
    26341 gcaacacacc aatcacgaag atgaagacaa acatagtgat gctggcctgc atgggaacaa
    26401 agaacatcgc gaggctaatc actgccagca gggcgttcgt ccaccagaag atagtgactt
    26461 tacatttcca gtcggtcaga ggttttgcca gtgcggaacc aatcaggtta cccacgcaat
    26521 aagtggtgag aaaagcgaca aacacttccg gcgtgcccaa aatccatgtg acgtaataca
    26581 tcatcgcccc accgcgtacg cacaccgcca ggatattgaa aatggttagt aaaccgacaa
    26641 tccgccactg gtcgttttgc cagatatcac gtaaatcttc ccgcatagac gttgttgtag
    26701 atggtgcttc aacgcgttct ttagtggtga agaaacaaaa tgccagcatc atgaatgcca
    26761 ccacggaaag gaccacgata ccgccctgga aaccgagtgg tttattatca ccgccaatta
    26821 aattaaccag tggcatcatc agaacagtag aaagcatgcc tcccgcggtc gccagcacaa
    26881 aacgccagga ttgcagcgag atacgctgag tcgggtcatt ggtgattaca ccacccaatg
    26941 cgcagtaagg gatattgacg acggtataaa gtaaggtaag ttagggtgta agtaattgct
    27001 gcatagatca ttttgccgtt catactgaga tctggcgtgc tataggccag tacacagacg
    27061 atcccgaatg gcagtgcgcc aaacagtacc cacggacgaa atttacccca gcgagagcgc
    27121 gttcgatcgg ccaacaaccc catgcaagga tcggaaatcg catccagtgc acgagcgacc
    27181 aaaaacatgg ttccgacaaa tccggcagga atgccaaaaa tatcggtata aaagaacatc
    27241 atatataaca ttacgttatc gaaaataatg tggctggcgg cgtctcccat gccataacca
    27301 attttctctt taacggacaa cacttcactc ctcatctgct tttccttgat gctatagggg
    27361 tgctgagacc gataacgcat tttgtaaacc atccctgtga gagcgaatat tccgatatct
    27421 ggttattaat ttatgattct tgttttatgt gatcgtggta gcgttaattc cgctcatata
    27481 tcattgtaaa atatgggttt tatataaatt ataatgcttt cgtgataata cgctgcgttt
    27541 attaggcgga aaaaactgat ctgggggatg tagaaactca aggaagtagc tataatgcgc
    27601 cccgcctcca tgtagcaatc gaggcgcgga agatcgtcgt ctccggtgag gcggctggac
    27661 ttcaaatcca gttggggccg ccagcggtcc cgggcaggtt cgactcctgt gatcttccgc
    27721 cagtttacct cttctgaact ctcccaatgt caataaatct cttctaatcc cttgtatatc
    27781 agtatttatt tgtatcctga ggtcaaccta cttcaccccg tatcaagttt cttttggggg
    27841 tactttaggg ggtgttattt cctattgaaa tacaagcacc cccataatag gaaaaatgtg
    27901 atacataaca acggctttta acaatatctt tgttatacaa ggggtgtatt gggggtatca
    27961 ggttagttac tggcagaaat ccccctcagg aacatgaatt catcaaaaag ccggaggatg
    28021 tcatggcatt aaccgatatc aaagtgaaaa ccgcaaaacc caaagacaaa ccctataagc
    28081 ttgctgatgg cggcggtatg tatctactga ttaacactaa tggttctaaa tactggcgca
    28141 tgaagtaccg ctttgctggt aaagaaaaga tgctttctat tggcgtatac cccgatgtaa
    28201 cgctagccga tgctcgcgag aaacgcagtg aagccaggaa actcctcgct gctggaggcg
    28261 atccaaggga ggcaaaaaag gaagaaaaaa tcgcgcagca aatgagtctg aaaaatacgt
    28321 tcgaggctat tgctcgcgaa tggcaccagt taaaagctga tcggtggtca ttacgctatc
    28381 gtgatgaaat cattgatacc tttgaaaaag acatttttcc ctacattggc aagcgcccta
    28441 ttgccgaaat caaaccaatg aagttactgg aagcactgcg taaaatggaa aaacgtggag
    28501 ctctggagaa aatgcgcaag gtccgccagc gctgtggtga ggtgttccgc tatgcaattg
    28561 ttactggccg ggcagactat aacccagccc ccgatcttgc cagtgcttta gctacaccga
    28621 aaaaagtaca ttttcccttt cttactgcta atgaacttcc ccacttcctc aacgatctgg
    28681 cgggttatac cggaagtata atcactaaga cagctactca gatcattatg ttaaccggcg
    28741 tacgaacaca agaactgcgt tttgcacgct gggaagatat cgattttgag acaaaattgt
    28801 gggagatccc ggcagaagta atgaagatga agcgacctca tattgtgccg ctctctgagc
    28861 aggtcattat gctatttaag cagcttgaac caatctcaaa acatcatccc ctagtcttta
    28921 tcggtagaaa tgatccccgc aaaccaatta gtaaggaaag cattaaccaa gtaattgaat
    28981 tacttggata taagggaaga ctcacagggc acggtttcag acacaccatg agtacaattc
    29041 tgcatgaaca aggctttaat tccgcctgga ttgaaatgca gttagctcat gtggataaaa
    29101 attccatcag gggtacctat aatcatgccc tgtatctcga tggccgccgt gaaatgatgc
    29161 aatggtacgc tgattacatt gattcactct ccagccggga aagttaagtc agaaatactt
    29221 ttccctgatg ttttcctcaa ttaagtattt tatcctctgg cctacaaggc tggaggattt
    29281 ttgttattat attatttttt tgcgtcttca aacttattac aaaaacataa ttaaaaacaa
    29341 attgacattt ttaaattggc aattacaaaa tacagccttg cagttatttc gataccttta
    29401 gcaaaggaat agttgattca gatataattt agcatttcaa tcatctgaca tcattataaa
    29461 aaatgagatt cacataaaga taaaattatt gttctcaagg aaagcatgaa ggggcttcgt
    29521 aagcaatatt cattcggtgc caagacagga cttgatacac agaatctcaa cttttatgct
    29581 ctcagactat atcaccacat gttttacagg ctcagaaaat gaacgataga ttatgctttg
    29641 aagtgcacga taacaagggt tatttcgtat ttccggatac ctggtttggc ccattgctgg
    29701 gggagtttga agaagtgctt gatgcatacg acgctgatga aatcagcgag acgagttaca
    29761 tcaacaaact ccgacgcctt gctcaacggg agccggactt tattgatata cacgctcatc
    29821 tggcatatgc atttctggaa cagaatgccc cgcgtaaggc actgaacgcc gctcttaaag
    29881 gactggcggc aggtaatcgt atcatcccgg aaagtttctg tggtgaaata atctggatgc
    29941 atcccgaaaa ccggccttat ctgcgcgcgc tttatgcagc aattctcgca aatgtgcact
    30001 tacagcgcca tcaggatgcg gttatgctca ctgataaaat actggcatac aatcctgaaa
    30061 ataatcaggg ggctcgctgg ttgttgggtt cagagctgtt acgcaccggc gatcatgagc
    30121 gagccttcag tgtactgaaa gagcatgctg atgaattttc accttactgg tatgaactcg
    30181 gtctcctgca ttttctgaac ggggaacatg ttaaggccgc cacagctttt cgccatggct
    30241 ttgccacaaa cacttacatc gcagaaatgc tgtgcggaaa tctgcatcca tttccacttg
    30301 ctgtgtggca tgacttctcc ggctctctgg atacggcaga agattactat gcaacatact
    30361 caccattatg gggacaatat ccggaagcat tgctgtttgt aaactggctc tataaccatt
    30421 cctccgtgct gcatgaacga tcagaaatca ttaaatgcgc cgaaatgctg attcaggaag
    30481 atgatttcga aatatgcgaa agtattttaa gacagcagga gcacctgtgg aaaagaattg
    30541 acaagacact ttctgaggaa attgtacaga aatgcagaaa tatgaacggt gaatatatct
    30601 ggccctggat attgcccttt tccgcagcag ggatgaaaca ttcaagcatc cagtatcagt
    30661 agatattgca ttagtgtaac tttaaaacaa gtaataattt attatccaat aataacactg
    30721 tgttccttcc ccaggtgatt accctggggt tattttctga aagtttcctt tgcaaaagga
    30781 tagcgttgta aattacttta acgaattact ccatgaactc attaaagttc tttcgtaata
    30841 ctttatcgtc aaacacgtta tagctggcat caaaaaccac agatttaccc tgccagcggg
    30901 taatcccctc ctctggcgtt ttcggttgaa ctggtatccc cgtccaacac aggcagccat
    30961 tataaacagt aaaaaattgt attcgctgtt atgaaatcaa tagtcctgac gtactgttga
    31021 atgtgcttgt gttctgaacc tcgtacaacc agtacatttg atatcgaatt ataaacaaaa
    31081 cattgcatcc aactttttta atatggacaa tagttgtcca tacggtggtt ttatattcga
    31141 taaatcaatg gatttcattg agtcacagga ctggtaatgg atgaaaatgc tttaaggttt
    31201 gcctcatact ggcgcaactc gcttgcagat gctgagtcag gaaagggctg ttttgaacgg
    31261 aaagacgccc aaaatttcac tcactggcat gggatagcgg cgggacgtct tgacgaagcg
    31321 attgtcaata aattttttaa gggagaaaaa gacgatgtcg aaactgtcaa tgtcatctta
    31381 cgcccaaaag tttatttccg gttactgcag catggtaagg accgttccgc aggcacgcct
    31441 gatattgtta caccgatagt gacgccagcc ttgctaagcc gtgagggttt tttatatccg
    31501 acgccagcga cctccattcc cagagacctg cttgaacctt tgccaaaagg agcattttcg
    31561 attggtgaga ttgggcagta tgacaaatac aagacgaccc atactgatcc tacccacgta
    31621 atgtggacac ggccctaagc gagattatgg ttttcaaatt gttccgggct gagaccgcca
    31681 caggcactgt gacgacgcca gcgattgtaa tcacactcga tataattaaa caccgttgcc
    31741 cgcattattt cccggctgct aaagcgttcc ccgtggatac attccacctt cagcgaatga
    31801 aagaagcttt ccacacaggc attgtcataa cagtagcctt tcgcactcat actgccacgc
    31861 aggttgtgtc gcttcagcat tgcctgataa tcccctgaac agtattgacc accacggtcc
    31921 gtatgaacaa tgacgctttc cgggcgtctt ctccgccaca acgccatttg cagtgcatca
    31981 caggccagtt gtgctgtcat tcgcggtgac atcgaccagc caatgacgga gcgtgaccac
    32041 aggtcgatga ctactgcgag atacaaccag ccctcatcgg tacgcaagta cgtgatgtca
    32101 cccgcccact tctggtttcg ggccactggc gctgaagtcc tgctccagca gattctccaa
    32161 tacgggcagg ccatgtgcac ggtagctgac cgggctgaac ttccggctgg ctttcgcccg
    32221 cagcccctga cgacgcaggc tggcggcaat ggttttaata ttgaactccg gcagttcgtc
    32281 agcaaggcgg ggagcaccgt atcgctgctt tgcctcaaag aatgccttat ggacagcggc
    32341 atcgcaggtg agccgaaact gttggcgcag gctcatctgg tgacgacgcc tgagccagac
    32401 ataccagccg ctgcgggcaa ctcgaagtac acgacacatc gctttgatgc tgaactctgc
    32461 ccgatgattt tcgataaaga catacttcat ttcaggcgct tcgcgaagta tgtcgcggcc
    32521 ttttggagga tggacagttc ctcagcctgc tccgccagtt gtcgtttaag gcggacattt
    32581 tcagcggcca gttcgctttc gcgctctgac gaactcattt gttgctgctg tttactgcgc
    32641 caggcataaa gctgagattc atacaggctg agttcacggg ctgcggcggc cacaccgatg
    32701 cgttcagcga gtttcagggc ttcgttacga aattcaggcg tatgttgttt acggggcttc
    32761 ttgctgattg atactggttt tgtcatgagt cacctctggt tgagagttta ctcacttagt
    32821 cctgtgtcca ctattggtgg gtaagatcac cctccattaa aactaaaacg ggcgctttct
    32881 gatatatcat ctgtaagcgc agtaatacca ttagtagcac ctctcggcgg cgcattgatt
    32941 attgaatatt ataactggaa tgttatattt cttgttatga tgctgttcag tattataata
    33001 atgttattat caccaaagat aactcatgca aatgagaaag taatatgtca aaacagtgca
    33061 tcctttgtga ccaaagagtt tatctgcggc actatacttg tctcattaat actcagttct
    33121 ctattttgct acatatcaat atcaccatca attttcatgc atacttatgg cctgagtact
    33181 tttagttact caattatttt ctccttctca gttttatttt tcataatcgg aaatcaatta
    33241 tcaaagattg aaaaattatc caacctatgg tttttattac ccaatagcca attttatcgc
    33301 attacaaata aatggttcaa aaacaggatt tgccgcatct attacaggtt ttactcaaac
    33361 aataagcgct ggtacaatca gtttcctgtc agtaaccgta agcgacaacg gtgttggtcc
    33421 cggaactact ttaggtcttg gttcactaat aacttcatta ctgtcaatag ccgtaatcgc
    33481 cattgcagga aataataaaa agtaagtgta ctctgactgc gtatgtatct acttgaaccg
    33541 ccccgg
//
LOCUS       DAECNO010000039        32635 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.039, whole genome shotgun sequence.
ACCESSION   DAECNO010000039 DAECNO010000000
VERSION     DAECNO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.039"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..956)
                     /locus_tag="KKO08_001880"
     CDS             complement(<1..956)
                     /locus_tag="KKO08_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBD6318144.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="KKO08_001881"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="KKO08_001881"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBD6318145.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KKO08_001882"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="KKO08_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KKO08_001883"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="KKO08_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBD6318146.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="KKO08_001884"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="KKO08_001884"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBD6318147.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="KKO08_001885"
     CDS             complement(5018..6346)
                     /locus_tag="KKO08_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBD6318148.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="KKO08_001886"
     CDS             complement(6354..8702)
                     /locus_tag="KKO08_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD6318149.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KKO08_001887"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="KKO08_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBD6318150.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KKO08_001888"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="KKO08_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBD6318151.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KKO08_001889"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="KKO08_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBD6318152.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KKO08_001890"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="KKO08_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBD6318153.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVCNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KKO08_001891"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="KKO08_001891"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD6318154.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="KKO08_001892"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="KKO08_001892"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBD6318155.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="KKO08_001893"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="KKO08_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBD6318156.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KKO08_001894"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="KKO08_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBD6318157.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="KKO08_001895"
     CDS             18273..19172
                     /locus_tag="KKO08_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBD6318158.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="KKO08_001896"
     CDS             19178..21610
                     /locus_tag="KKO08_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBD6318159.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="KKO08_001897"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="KKO08_001897"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBD6318160.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="KKO08_001898"
     CDS             22723..23988
                     /locus_tag="KKO08_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBD6318161.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="KKO08_001899"
     CDS             complement(24229..25821)
                     /locus_tag="KKO08_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBD6318162.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="KKO08_001900"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="KKO08_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD6318163.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="KKO08_001901"
     CDS             complement(27018..28136)
                     /locus_tag="KKO08_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBD6318164.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="KKO08_001902"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="KKO08_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBD6318165.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="KKO08_001903"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="KKO08_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBD6318166.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /gene="opgE"
                     /locus_tag="KKO08_001904"
     CDS             29186..30769
                     /gene="opgE"
                     /locus_tag="KKO08_001904"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBD6318167.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="KKO08_001905"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="KKO08_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBD6318168.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="KKO08_001906"
     CDS             complement(31385..31450)
                     /locus_tag="KKO08_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318169.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="KKO08_001907"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="KKO08_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBD6318170.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8168 a         8701 c         7883 g         7883 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcagaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaagtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatacagaa tttctcgcta cttttcctct acacc
//
LOCUS       DAECNO010000040        32143 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.040, whole genome shotgun sequence.
ACCESSION   DAECNO010000040 DAECNO010000000
VERSION     DAECNO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.040"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="KKO08_001908"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="KKO08_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /locus_tag="KKO08_001909"
     CDS             1128..1235
                     /locus_tag="KKO08_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318171.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="KKO08_001910"
     CDS             complement(1288..2046)
                     /locus_tag="KKO08_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBD6318172.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="KKO08_001911"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="KKO08_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBD6318173.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="KKO08_001912"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="KKO08_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBD6318174.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="KKO08_001913"
     CDS             3759..4619
                     /locus_tag="KKO08_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBD6318175.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="KKO08_001914"
     CDS             complement(4660..5196)
                     /locus_tag="KKO08_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBD6318176.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="KKO08_001915"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="KKO08_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBD6318177.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="KKO08_001916"
     CDS             6174..6764
                     /locus_tag="KKO08_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD6318178.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /gene="tcyP"
                     /locus_tag="KKO08_001917"
                     /pseudo
     CDS             6897..8287
                     /gene="tcyP"
                     /locus_tag="KKO08_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(8291..9107)
                     /locus_tag="KKO08_001918"
                     /pseudo
     CDS             complement(8291..9107)
                     /locus_tag="KKO08_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="KKO08_001919"
     CDS             complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="KKO08_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBD6318179.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9830..12091
                     /gene="katE"
                     /locus_tag="KKO08_001920"
     CDS             9830..12091
                     /gene="katE"
                     /locus_tag="KKO08_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBD6318180.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLKPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="KKO08_001921"
     CDS             complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="KKO08_001921"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBD6318181.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12909..14261)
                     /gene="celF"
                     /locus_tag="KKO08_001922"
                     /gene_synonym="chbF"
     CDS             complement(12909..14261)
                     /gene="celF"
                     /locus_tag="KKO08_001922"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBD6318182.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="KKO08_001923"
                     /pseudo
     CDS             complement(14366..15208)
                     /gene="chbR"
                     /locus_tag="KKO08_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="KKO08_001924"
     CDS             complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="KKO08_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBD6318183.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="KKO08_001925"
     CDS             complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="KKO08_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBD6318184.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="KKO08_001926"
     CDS             complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="KKO08_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBD6318185.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="KKO08_001927"
     CDS             complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="KKO08_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBD6318186.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18218..19045
                     /gene="nadE"
                     /locus_tag="KKO08_001928"
     CDS             18218..19045
                     /gene="nadE"
                     /locus_tag="KKO08_001928"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBD6318187.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19275..20162
                     /gene="cho"
                     /locus_tag="KKO08_001929"
     CDS             19275..20162
                     /gene="cho"
                     /locus_tag="KKO08_001929"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBD6318188.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20122..20697)
                     /gene="ves"
                     /locus_tag="KKO08_001930"
     CDS             complement(20122..20697)
                     /gene="ves"
                     /locus_tag="KKO08_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBD6318189.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20900..21385)
                     /gene="spy"
                     /locus_tag="KKO08_001931"
     CDS             complement(20900..21385)
                     /gene="spy"
                     /locus_tag="KKO08_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBD6318190.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21715..22683)
                     /gene="astE"
                     /locus_tag="KKO08_001932"
     CDS             complement(21715..22683)
                     /gene="astE"
                     /locus_tag="KKO08_001932"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBD6318191.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22676..24009)
                     /gene="astB"
                     /locus_tag="KKO08_001933"
                     /pseudo
     CDS             complement(22676..24009)
                     /gene="astB"
                     /locus_tag="KKO08_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24006..25484)
                     /gene="astD"
                     /locus_tag="KKO08_001934"
     CDS             complement(24006..25484)
                     /gene="astD"
                     /locus_tag="KKO08_001934"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBD6318192.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25481..26515)
                     /gene="astA"
                     /locus_tag="KKO08_001935"
     CDS             complement(25481..26515)
                     /gene="astA"
                     /locus_tag="KKO08_001935"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBD6318193.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26512..27732)
                     /gene="astC"
                     /locus_tag="KKO08_001936"
                     /pseudo
     CDS             complement(26512..27732)
                     /gene="astC"
                     /locus_tag="KKO08_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28178..28984
                     /gene="xthA"
                     /locus_tag="KKO08_001937"
     CDS             28178..28984
                     /gene="xthA"
                     /locus_tag="KKO08_001937"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBD6318194.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29151..29859
                     /locus_tag="KKO08_001938"
                     /pseudo
     CDS             29151..29859
                     /locus_tag="KKO08_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29864..30541
                     /gene="ydjY"
                     /locus_tag="KKO08_001939"
     CDS             29864..30541
                     /gene="ydjY"
                     /locus_tag="KKO08_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBD6318195.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30556..31263
                     /locus_tag="KKO08_001940"
     CDS             30556..31263
                     /locus_tag="KKO08_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBD6318196.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31263..31811
                     /locus_tag="KKO08_001941"
     CDS             31263..31811
                     /locus_tag="KKO08_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBD6318197.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31821..>32143
                     /locus_tag="KKO08_001942"
     CDS             31821..>32143
                     /locus_tag="KKO08_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD6318198.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8247 a         8242 c         7612 g         8042 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttccagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattaacca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaatcatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     8401 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     8461 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     8521 caccacttcc cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg
     8581 atattatcga aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc
     8641 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8701 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8761 atcccaacaa aattctcatt aatatctctt atccgaaact cactgccata aagattatta
     8821 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8881 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8941 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     9001 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     9061 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     9121 ttattatctt atttaaatct aagtcattta caatataagt ttaagagcga cgccacagga
     9181 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     9241 taattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     9301 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     9361 caaaaccggg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     9421 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     9481 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9541 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9601 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9661 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9721 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9781 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
     9841 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9901 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9961 gccaggtgca caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa
    10021 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
    10081 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
    10141 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
    10201 acgtattgtt catgcacgcg gctcagcagc tcacggttat ttccagccat ataaaagctt
    10261 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
    10321 taggttctct accgttcagg gtggagctgg ctctgccgat accgtgcgtg atatccgtgg
    10381 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
    10441 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
    10501 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10561 tctgaaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10621 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10681 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10741 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10801 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10861 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10921 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10981 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    11041 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    11101 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    11161 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    11221 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    11281 ataccaagag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    11341 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    11401 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    11461 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    11521 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11581 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
    11641 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccatgaa
    11701 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11761 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11821 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11881 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11941 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    12001 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    12061 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    12121 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    12181 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    12241 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    12301 ggatcacccc gatggcttgc gtcatccagc acttgcagga acagtgcctc gctaatctct
    12361 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattaaccgg taaatcacca
    12421 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    12481 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12541 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12601 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12661 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12721 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12781 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12841 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12901 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12961 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    13021 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    13081 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    13141 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgtgcccca gcttacaggt catttctacc
    13201 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    13261 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    13321 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
    13381 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    13441 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    13501 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13561 ccagatactt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13621 ccatttacca gcacatcctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13681 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13741 atattacaca cgccgataaa acgtttaaat ccggtatgac gataaacggc ttcagtaacc
    13801 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13861 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13921 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13981 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    14041 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    14101 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    14161 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    14221 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    14281 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    14341 tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatatc
    14401 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    14461 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgtttttttg
    14521 caaaattaat acggatctca ttaataatct gcattggtgt tttgccataa tatcgttgag
    14581 tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg
    14641 attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc cactacggta
    14701 catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa
    14761 ccatctcaac aaactcttcc agccctgttt cgcggaaatt taatgaagaa atcactgttt
    14821 ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagcaaca aagcaataag
    14881 gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc
    14941 gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat
    15001 cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata
    15061 ctagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat
    15121 agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt
    15181 ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc
    15241 tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg
    15301 tggaccagca ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc
    15361 gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat ggctttcgct
    15421 gcggcaaaat cgccctgctt cgcctgtttc aatgcggcat acgccaggct acgcgcttgt
    15481 ccagagttga tgataagccc catcaccact tcttccagct cttcagcttc tgtttgcgta
    15541 tcaggaatat tatcgagatc catcatacat cgttcctctc tcttcttacc ggcaagatta
    15601 cccgtaccgg catcgattaa aatttcaggg gttagcgata tcttcttcgc tctcttcttt
    15661 atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc
    15721 gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
    15781 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaatgatgcc
    15841 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
    15901 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
    15961 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
    16021 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
    16081 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
    16141 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
    16201 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
    16261 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
    16321 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
    16381 ggaaagaata ataaagccgg gaattaatgc cgagaaagaa cgcgatactg aagcaggtac
    16441 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
    16501 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
    16561 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
    16621 cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
    16681 tgccattaaa gacattattc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
    16741 accattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga
    16801 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
    16861 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
    16921 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
    16981 atacccttaa aaaccgcaat ttaaatatta cggtattgat ttatgaaata actctttgac
    17041 gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt
    17101 aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt
    17161 gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac
    17221 gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac
    17281 cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag agatagacat
    17341 gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat
    17401 tatctgtcag ccagacactc cgcaagcctt gacctgcttc catgctctgg gtaacttgcg
    17461 aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt
    17521 atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt
    17581 cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc
    17641 cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag
    17701 tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg
    17761 cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac
    17821 cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg
    17881 cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac
    17941 gatcataagc tgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg
    18001 ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata
    18061 cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat
    18121 gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct
    18181 ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat
    18241 aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga
    18301 ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag
    18361 cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg
    18421 ccaggaaact cgcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca
    18481 ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac
    18541 cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag caggcattga
    18601 actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag
    18661 cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac
    18721 cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt atcgtctcaa
    18781 caaacgccag ggtaaacagt tactgacggc attaggttgc ccggaacacc tttataagaa
    18841 agccccaact gccgatcttg aagatgatcg cccttctctg ccagatgaag tggcactcgg
    18901 cgtgacctat gacaatatcg acgactatct ggaagggaaa aacctacctg aacaggtcgc
    18961 cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaacgt
    19021 tttcgatgat ttctggaaaa agtaataatt tgcacctatt ggattgtgcg aaaaagagta
    19081 atttgttcac gccggatgcg gcgtgaacgc cttattcgac ctacaaaact atgcaaattc
    19141 aatacattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt
    19201 gtcatcaacc cgcctctttt ttaatcgcct cccgcctgtt acactggata gataaccagc
    19261 attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg
    19321 caatttatga atatcccgaa catttacatt cattccttaa tgacttaccc acccgaccag
    19381 gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta
    19441 atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc atgctacggc
    19501 aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg ctccttgaag
    19561 cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc
    19621 tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt
    19681 tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat
    19741 tgcagagcat cgctgatgaa caaaaacttt gttatggcct gttgggactg gaaccgttaa
    19801 gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta
    19861 aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgagtgg
    19921 tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca gcacccggaa atgactcaat
    19981 atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa
    20041 cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc
    20101 tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt
    20161 gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc
    20221 gaaccagcac gcgccctggt cggtagtgag cagtttgtcg cccagttgcc acgccccgtt
    20281 aatcacaaac accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct
    20341 gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg acatctgccc
    20401 tgccgtcagt ttcgctttta ccacctggtc cgccgtaaag gcaaaaggct gtaacggttt
    20461 taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt
    20521 cactatcctt tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat
    20581 gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc
    20641 accggcagcg tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact
    20701 cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatga ttatcggcaa
    20761 ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa
    20821 gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt
    20881 aagttttttg gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac
    20941 gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga
    21001 tgttgtaaat tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt
    21061 ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt
    21121 catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca
    21181 tgatttcgtg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt
    21241 cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg
    21301 cggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa
    21361 caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat ttattacggc
    21421 tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat
    21481 cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa
    21541 aaagaaaatt tgatggagag tgatgagaga atattacaac acgatgattt tgcagagatt
    21601 atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta
    21661 ttaatttata agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct
    21721 tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa
    21781 cacatactct acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt
    21841 tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg
    21901 cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc
    21961 gacactctca ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag
    22021 atcgttttgc ccaaagggca acgctttgcc aagttccaac gtacagacca gcgcgccaaa
    22081 atgtctgacg ctgaaatggg taaacgtacc accaggctcc tgatggaaca ccagcgcctc
    22141 cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg
    22201 taatacaccg aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca
    22261 acgcacagat tctttgccct ggtcataaaa atcttccagg cactgttcca gttcgcgcgc
    22321 ccgacaggtt tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcggttcat
    22381 atcgctatgg caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag
    22441 caaccgccaa cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat
    22501 ttccacaggt gccgtctcat taccgtggat ccccgcagaa atcaccagcg cgccttgcgg
    22561 tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt
    22621 gatttctcgc tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc
    22681 cattgccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca
    22741 atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag
    22801 taagacgatc gcggtagtaa cgatccaccc agtcattgag cacattaaac aaggtatcgt
    22861 tcatcatcac cgccggattc accgcccggc gttcttcttc tgtcaatacc ccccgcaacc
    22921 gcaggcacgc cgggccgccg ccattcgcca tgctttcacg taagtcaaag acttttagtt
    22981 cactaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgct
    23041 cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc aacagttggc
    23101 tgttaaacag ataggtagac accgcatccg acacggaaac ctgagttgcc ggaacttcta
    23161 tcgccataaa accgttcacc cgcgtgccag taactgtgac tggcgagcga acgcctgttg
    23221 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgctctg
    23281 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattaag
    23341 ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
    23401 atcattgcct tcttctcgcc cgtagacaaa aagtagcata cccggttcac cgtaatgacc
    23461 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gcggcaacgc
    23521 cgaatggacg ctaaatttct cttcgtcctt aaaaatcgct tttaacagcg attcagtgac
    23581 gggcgcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
    23641 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
    23701 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
    23761 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
    23821 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
    23881 ttttgccgcc agtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
    23941 aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca
    24001 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt ggcgggcaat
    24061 gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag
    24121 gcgctggggc gatggttacc ggaagcacca atgccgccga atggggcggt actggcagca
    24181 ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
    24241 aatttttccc gctcggggga aaccagaccg caaaagagtc cgaagcgagt gttgttcgcc
    24301 atccgaatcg cctcatcaaa agtatcataa cgccagacgc gcaaaagcgg cccgaacacc
    24361 tcttcatccg gcacaccgcc aacgcccgtc atttcaatga tccctggtgt cagcaacgat
    24421 gtccctgctt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc
    24481 tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
    24541 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagactagca
    24601 agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt
    24661 tgcccgactg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
    24721 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
    24781 tgaccggaga gctggcgatg cagctggtag ccggtattgg cgctaccggt aaacagcaaa
    24841 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
    24901 ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
    24961 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
    25021 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
    25081 tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt cactacgctg ctcgccggta
    25141 cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
    25201 gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa ttcggcttta
    25261 ttgctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagc
    25321 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgttcgac ctgagcggca
    25381 tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
    25441 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
    25501 cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg
    25561 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg cgccagcacc acgcggaaat
    25621 ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt
    25681 ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg tcacactcaa
    25741 gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca
    25801 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
    25861 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
    25921 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
    25981 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
    26041 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
    26101 aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
    26161 tgccggtgtg atcgttacta agaaacagcg tcggcaatgc gttatagaca ttcagctctt
    26221 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg
    26281 ccacctcaat agcgcaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
    26341 catagccctg ctcgcttttg ggcagttcac cttgccaggt tttgattgcc ctttcgatac
    26401 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
    26461 caagctgcat cagcgccgag acatctgagc gctcaacggg acggatgacc atcatgatga
    26521 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
    26581 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac
    26641 catcacgcct gctttcgccg cttccagaga gatctgtttc gcttgcccgg cgtaatcggc
    26701 attcagtaca cagccaatca acaaacctaa gccgcgaact tcactgaaca aaccatagcg
    26761 gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
    26821 catctctggt gtgttgatga gctccagcac tttgcctgct accgccgagg ccagcggatt
    26881 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
    26941 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtggtta acagatcggg
    27001 cgtcacgccg tagtgcatat aggcatacag ctcaccagtg cgcccgacgc cggtttgtac
    27061 ctcatcaaaa atcagcaagg cattgtggcg gtcacacaat tcacgcagac cttgtaaaaa
    27121 cgcgttgctg gctggcacca caccgccttc gccctagatg ggctcgacaa tcaccgcaca
    27181 ggtggcgtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    27241 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    27301 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccactct tatggctgcc
    27361 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    27421 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    27481 cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt
    27541 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    27601 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    27661 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    27721 tggctgagac atagcgacct ctacagtaaa taattcgtta tttgcatgtt aacaataagt
    27781 aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    27841 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    27901 atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat
    27961 gcagaagtaa aatataattc tagaattgtg atcattgacg aaatttactg gaaattactg
    28021 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatca ttgacatcat
    28081 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    28141 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    28201 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    28261 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    28321 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    28381 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    28441 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    28501 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    28561 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    28621 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    28681 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    28741 caggctgatg agctgggggg tggtcgatac cttccgccat gctaatccgc aaacagcaga
    28801 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    28861 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    28921 tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    28981 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    29041 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt
    29101 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    29161 agcgtaactt tctgtttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt
    29221 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatt cgccagagcg
    29281 gattgttcgg ttatagcctc tatattctgt tattcatcaa tgccccctct ttctgttacc
    29341 aggaagcata ctggtgatcg ctggaggaat agtttttggc ccgctcttag gacacaactc
    29401 tcattaattg ccgcaacgct ggcctcctcg tgctcattcc tgctggcgcg ctggctggga
    29461 cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc
    29521 attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac
    29581 aatattcaaa attacgctta cggattcacc accatcgcct tctggcctta tacccttatt
    29641 tcggcactca cgaccctgcc cggtattgtt atttataccg tgatggcaag cgatctcgcc
    29701 aatgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt
    29761 attctcatcc agctcgcaaa actctacgcc cgacacaaac atgtggatct gtctgcttcg
    29821 cgccgcagcc cacttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc
    29881 agtgtcatgg aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg
    29941 ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca
    30001 accgctgcgc gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg
    30061 tttcctcacc gacgacactc gtcacggtat tgtgtttaaa gatggctcca acggacataa
    30121 atcgctgttt atggcttatg cgcccccgaa agcattttat gaagccctga aagaggcagg
    30181 tggtacgccg ggcgaaaaca tgacgatgga taataaagaa acgactcatg tcacaggcag
    30241 caaactggat atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt
    30301 gattgttgac agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc
    30361 agaagagaag aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag
    30421 caatgcaaca tacacttatg gtgcggtgga aaaacacggc gaagtgaaat tcaaaggcaa
    30481 tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata
    30541 aagccaagat aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc
    30601 ctcatcatcc tgttattcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag
    30661 tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt
    30721 attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc
    30781 attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg
    30841 ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt
    30901 atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac
    30961 agtatggacg gctttttcac tcgctacggc aaacacacca tcctggtctg tcggttattg
    31021 ccttttgtcc ctttcgatcc aatcagctat gctgccggtc tgacttcaat acgttttcgc
    31081 tcgtttttta tcgccaccgg gcttggtcaa ttaccggcga ctattgttta ttcctgggca
    31141 ggcagcatgt taacaggcgg tactttctgg tttgtcaccg aactgtttat tctgtttgcc
    31201 ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa gaggaatgac
    31261 tgatgggttt accgccgctt agcaaaattc ctttcatttt acgtccgcag gcgtggctgc
    31321 atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg atcccgttta
    31381 tcttttattt ggtgtcaatg tttgttgggt ggctggagcg caaacgctca ccgctcgatc
    31441 cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg
    31501 tggatatcac cagtatgaaa gtcgccgagc gtaccggcag caccgataaa ctgctggcag
    31561 tggctgactg gcggcaaagc ccgctcttta gcgatgaaga acggctggcg ctggagtacg
    31621 ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc cctgcgtacc cgactggctg
    31681 cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt
    31741 cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa
    31801 aacgttctta aggagggaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt
    31861 tttctttggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa
    31921 ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta
    31981 gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctc gcggatgccg
    32041 cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg tcgtaaaacg ggcggctcgg
    32101 tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaa
//
LOCUS       DAECNO010000041        32125 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.041, whole genome shotgun sequence.
ACCESSION   DAECNO010000041 DAECNO010000000
VERSION     DAECNO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.041"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KKO08_001943"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="KKO08_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBD6318199.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="KKO08_001944"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="KKO08_001944"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBD6318200.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="KKO08_001945"
     CDS             complement(3736..4092)
                     /locus_tag="KKO08_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD6318201.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="KKO08_001946"
     CDS             complement(4096..4512)
                     /locus_tag="KKO08_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBD6318202.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KKO08_001947"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="KKO08_001947"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBD6318203.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="KKO08_001948"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="KKO08_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KKO08_001949"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="KKO08_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBD6318204.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KKO08_001950"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="KKO08_001950"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBD6318205.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KKO08_001951"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="KKO08_001951"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD6318206.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="KKO08_001952"
     CDS             10521..11504
                     /locus_tag="KKO08_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBD6318207.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KKO08_001953"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="KKO08_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBD6318208.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KKO08_001954"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="KKO08_001954"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBD6318209.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="KKO08_001955"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="KKO08_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="KKO08_001956"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="KKO08_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBD6318210.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="KKO08_001957"
     CDS             complement(15306..15515)
                     /locus_tag="KKO08_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBD6318211.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KKO08_001958"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="KKO08_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBD6318212.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KKO08_001959"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="KKO08_001959"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBD6318213.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KKO08_001960"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="KKO08_001960"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBD6318214.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="KKO08_001961"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="KKO08_001961"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBD6318215.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KKO08_001962"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="KKO08_001962"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBD6318216.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KKO08_001963"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="KKO08_001963"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBD6318217.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="KKO08_001964"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="KKO08_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="KKO08_001965"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="KKO08_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBD6318218.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="KKO08_001966"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="KKO08_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBD6318219.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="KKO08_001967"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="KKO08_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBD6318220.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="KKO08_001968"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="KKO08_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBD6318221.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="KKO08_001969"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="KKO08_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBD6318222.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="KKO08_001970"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="KKO08_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBD6318223.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8174 a         8246 c         7780 g         7925 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttaaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcaacgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga ttaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gcctt
//
LOCUS       DAECNO010000042        31767 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.042, whole genome shotgun sequence.
ACCESSION   DAECNO010000042 DAECNO010000000
VERSION     DAECNO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.042"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1359)
                     /gene="yeaG"
                     /locus_tag="KKO08_001971"
     CDS             complement(<1..1359)
                     /gene="yeaG"
                     /locus_tag="KKO08_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBD6318224.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQ"
     gene            1795..2541
                     /gene="mipA"
                     /locus_tag="KKO08_001972"
     CDS             1795..2541
                     /gene="mipA"
                     /locus_tag="KKO08_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBD6318225.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2631..3392
                     /locus_tag="KKO08_001973"
     CDS             2631..3392
                     /locus_tag="KKO08_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBD6318226.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3443..4327)
                     /locus_tag="KKO08_001974"
     CDS             complement(3443..4327)
                     /locus_tag="KKO08_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBD6318227.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4411..5406)
                     /gene="gapA"
                     /locus_tag="KKO08_001975"
     CDS             complement(4411..5406)
                     /gene="gapA"
                     /locus_tag="KKO08_001975"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD6318228.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5748..6161
                     /gene="msrB"
                     /locus_tag="KKO08_001976"
     CDS             5748..6161
                     /gene="msrB"
                     /locus_tag="KKO08_001976"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBD6318229.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6203..6475
                     /locus_tag="KKO08_001977"
     CDS             6203..6475
                     /locus_tag="KKO08_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBD6318230.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6845..7921
                     /locus_tag="KKO08_001978"
     CDS             6845..7921
                     /locus_tag="KKO08_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD6318231.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7948..9327
                     /locus_tag="KKO08_001979"
     CDS             7948..9327
                     /locus_tag="KKO08_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6318232.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9344..10389
                     /locus_tag="KKO08_001980"
                     /pseudo
     CDS             9344..10389
                     /locus_tag="KKO08_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10410..11246
                     /locus_tag="KKO08_001981"
     CDS             10410..11246
                     /locus_tag="KKO08_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBD6318233.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11251..12198
                     /locus_tag="KKO08_001982"
     CDS             11251..12198
                     /locus_tag="KKO08_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBD6318234.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12208..13188
                     /gene="ydjG"
                     /locus_tag="KKO08_001983"
     CDS             12208..13188
                     /gene="ydjG"
                     /locus_tag="KKO08_001983"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBD6318235.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13325..14083
                     /locus_tag="KKO08_001984"
     CDS             13325..14083
                     /locus_tag="KKO08_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6318236.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14201..15559
                     /locus_tag="KKO08_001985"
     CDS             14201..15559
                     /locus_tag="KKO08_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6318237.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15652..16293)
                     /gene="pncA"
                     /locus_tag="KKO08_001986"
     CDS             complement(15652..16293)
                     /gene="pncA"
                     /locus_tag="KKO08_001986"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBD6318238.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGIEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16304..17320)
                     /gene="ansA"
                     /locus_tag="KKO08_001987"
     CDS             complement(16304..17320)
                     /gene="ansA"
                     /locus_tag="KKO08_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBD6318239.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17487..19343)
                     /gene="sppA"
                     /locus_tag="KKO08_001988"
     CDS             complement(17487..19343)
                     /gene="sppA"
                     /locus_tag="KKO08_001988"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBD6318240.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19504..20055
                     /locus_tag="KKO08_001989"
     CDS             19504..20055
                     /locus_tag="KKO08_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBD6318241.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20172..21215
                     /gene="selD"
                     /locus_tag="KKO08_001990"
     CDS             20172..21215
                     /gene="selD"
                     /locus_tag="KKO08_001990"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBD6318242.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21220..23181
                     /gene="topB"
                     /locus_tag="KKO08_001991"
     CDS             21220..23181
                     /gene="topB"
                     /locus_tag="KKO08_001991"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBD6318243.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23308..24348
                     /locus_tag="KKO08_001992"
     CDS             23308..24348
                     /locus_tag="KKO08_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBD6318244.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24465..25808)
                     /gene="gdhA"
                     /locus_tag="KKO08_001993"
     CDS             complement(24465..25808)
                     /gene="gdhA"
                     /locus_tag="KKO08_001993"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBD6318245.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26044..26316
                     /locus_tag="KKO08_001994"
     CDS             26044..26316
                     /locus_tag="KKO08_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBD6318246.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26282..26689)
                     /gene="nudG"
                     /locus_tag="KKO08_001995"
     CDS             complement(26282..26689)
                     /gene="nudG"
                     /locus_tag="KKO08_001995"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBD6318247.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26776..27396
                     /locus_tag="KKO08_001996"
     CDS             26776..27396
                     /locus_tag="KKO08_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD6318248.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27405..28712)
                     /gene="ynjE"
                     /locus_tag="KKO08_001997"
                     /pseudo
     CDS             complement(27405..28712)
                     /gene="ynjE"
                     /locus_tag="KKO08_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(28779..29432)
                     /locus_tag="KKO08_001998"
     CDS             complement(28779..29432)
                     /locus_tag="KKO08_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD6318249.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29432..30949)
                     /locus_tag="KKO08_001999"
                     /pseudo
     CDS             complement(29432..30949)
                     /locus_tag="KKO08_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(30922..31767)
                     /locus_tag="KKO08_002000"
                     /pseudo
     CDS             complement(30922..31767)
                     /locus_tag="KKO08_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7957 a         7603 c         8240 g         7967 t
ORIGIN      
        1 ctgctgttcc aggacgtaga acagatggac cgggtttgcc gccacttcta catgatcgaa
       61 gttgaacacg cgggagagga tcttaaacgc aaaacgcgtc gacagaccgt tcatcccttc
      121 atcgacaccg gcgtagtcac gatattcctg atacgacttg gctttcggat cggtgtcttt
      181 cagactttcg ccatcataaa cccgcatctt tgaataaatg ctggagtttt ctggctcttt
      241 caggcgcgaa agaatggaaa aacgtgacag tgtttcgagc gtgccagggg cgcatggggc
      301 gtgagtcaat tcactgtgat taagcaattt ctcgtagatt ttgatctctt cggaaatgcg
      361 caagcaatac ggcaccttca cgatgtaaac acggtcgagg aaggcttcgt tgtttttgtt
      421 attacggaaa gtgacccatt cggacttgtt cgagtgggcg agaataatcc cgttgaacgg
      481 cagggcggag ataccttccg tcccgttgta gttaccttcc tgagtggcgg ttaacaaggg
      541 atgcagcact tttatcggtg ctttaaacat ctcaacgaat tccatgatcc cctgattggc
      601 gcggcacagc gcaccggaat agccgtaggc gtccgggtca ttctgcgcgt agtgttcgag
      661 tttacgaata tcgactttcc caaccagcgc ggagatgtcc tggttgttct catcaccggg
      721 ttccgttttg gcgatagcaa tttgttgcag aattgacggc cagaccttca ctacccggaa
      781 cttagtgata tcgccaccaa attcatgcag gcgttttgcc gcccatggcg acatgatggt
      841 gccgagataa cggcgaggaa tgccatactc tttttccaga atctgagcat cttcctgcgg
      901 attgaaaaga cagaacggat gatcgttgac cgggctacgc tcaccgttgg cgctcaatac
      961 ataaatcggt acgagctgca ttaatgattt cagtcgctca gcaagcgatg atttaccccc
     1021 acccacaggc cccagcagat acaggatttg tttcttctct tccagcccct gagccgcgtg
     1081 tttcagataa gagacaatct gttcaatcgc gtcttccatg ccgtaaaact cttcaaatgc
     1141 cggataacgt gcaatgaccc ggttagaaaa gagtcgagaa agtctggggt cctgggctgt
     1201 atcgaccatg acaggctcac cgatagccat caatagccgc tcagccgcgt tggcataagc
     1261 actgcgatct tgccgacaag tggtaagaaa ctcctgcagt gtgaactctt cgtccttggc
     1321 agcttcatat cgctggcgat agtgatcaaa tatattcatg gtatgccgtc ctttcgtttt
     1381 ttagcacagg ttaagagccg ttcatatgaa tagtagaggc tcccggaaga ggtaaactga
     1441 acgacgtctc ttgctaaaac acagcaactc tcatgccatt ttatcgacga tgagaacaac
     1501 gtgcccggtg atacaccttc aaaattaagc gtagatggca aatggaaaac ttgcctgcac
     1561 ggctttctta atttcaatgg catatcaaca actcatccat aaattttgca taattaatgt
     1621 aaagaccagg ctcgccagta acgctaaatc catttggctg taagcgcggt gtcatccgcg
     1681 gcaggaaaat taaacagtta ctttaaaaaa tgaaaacgta aaaaggttgg gtttcgatgt
     1741 attgacgggt aaactttgtc gcccgctaaa catttgttta aggaatgatt aattgtgacc
     1801 aaactcaaac ttctggcact tggagtgctt atcgcaacgt ctgcaggcgt agcgcacgct
     1861 gaaggtaaat tttccctggg cgcaggcgta ggtgtcgttg agcacccata taaagattac
     1921 gataccgatg tttacccagt accggtaatc aactatgaag gcgataactt ctggttccgt
     1981 ggcttaggtg gtggttacta cctgtggaat gacgcaacgg ataaactttc aattaccgct
     2041 tactggtcgc cgctttactt caaagcgaaa gacagtggcg atcaccaaat gcgtcacctg
     2101 gatgaccgta agagcaccat gatggctggt ctgtcttatg ctcactttac ccagtacggt
     2161 tacctgcgta ccaccctggc tggcgatacc ctggataaca gcaacggcat tgtctgggat
     2221 atggcctggt tgtatcgtta caccaacggt ggcctgaccg tgactccggg tattggtgtg
     2281 cagtggaaca gcgaaaacca gaacgaatac tattatggcg tatcgcgcaa agagtccgct
     2341 cgcagcggtc tgcgtggcta taacccgaat gacagctgga gcccttacct tgagctgagc
     2401 gccagctaca acttcctcgg cgactggagt gtttacggta ccgcgcgcta cacccgtctg
     2461 tctgatgaag ttactgacag cccgatggtg gataaatcct ggactggcct gatttctacc
     2521 gggatcacct acaaattctg ataatgcatc tttccagggc attttgtgca taaaaacagg
     2581 gcgtcacgcc ctgttttgca ttacggtggt gcaatacctg ggaggaaaca atgcaacaaa
     2641 aaatgattca atttagtggc gatgtctcac tgccagccat agggcaggga acatggtata
     2701 tgggcgaaga tgccagtcag cgcaaaacag aagttgctgc actacgcgca ggcattgaac
     2761 tcggtttaac cctcattgat accgccgaaa tgtatgccga tggcggtgcc gaaaaggtgg
     2821 ttggggaagc attaaccggt ctgcgagaga aggtctttct cgtctctaaa gtctatccgt
     2881 ggaatgctgg cgggcaaaaa gcgataaatg catgcgaagc cagtttacgc cgtctcaata
     2941 ctgactatct cgatctctac ttattacact ggtctggcag tttcgctttt gaagagactg
     3001 ttgccgcgat ggaaaaattg atcgcccagg gaaaaatccg tcgctggggc gtttctaacc
     3061 ttggttatgc cgatatgcag gaactctggc agctgccggg gggaaatcag tgtgccacta
     3121 atcaggtgct ttaccatctc ggttcacgag ggattgagta cgatctactc ccctggtgcc
     3181 agcaacagca gatgccggtg atggcttaca gtccattagc ccaggccggg cggttgcgca
     3241 atggactgtt aaaaaacgcg gtagtcaacg aaattgcaca tgtccaacaa catgcagctg
     3301 cgcttgaggt cgaactttcg tcagcggaat tagctatgct ggataaggca tatccggcac
     3361 caaaaggaaa aactgcgctg gatatggtgt gatgttgata cgtgccggat gcatcgcacc
     3421 cggcacacaa cattaacgca aattaacgtt tggcaacgcg aatggattgc gccagatgtg
     3481 caggtttctc tttggtgact ttttgcgttt ctgaagcgta agccgtttct acacaaacaa
     3541 aggtcttgta gccatcgtcc ggcatgtcgc ccatgctaac tgaaagcgcc ggtcccgggt
     3601 tccagccgac aacgttcaga tgatgctggt ggcctacggc gataatacga ttcagcgctt
     3661 catcattaat cacgctgcaa tcttgcggat tcagatacac gcgatcggta cggtcaggga
     3721 aggtctgaat accgtcggtc agtacatctt ctttcgcgtc attcacttta tcaatgaagc
     3781 gatcgcccag cccactgacg cttaccttag cgatatcacc cacgttaaag taggtatgca
     3841 gggcagaggt ggtttcaaat tcgccatgtg attcaagatc gatttcacag gttttaccca
     3901 cgcggaaatg cgctaacagc gtaaagtcgt gcggccagaa ttttttcgtc tcttcgcttt
     3961 gtgtcaattc aaaagtcagc gctacgccat cagcatcttc atgatgtgat ttcagcgtcc
     4021 acggcaggtt gcgggcaaaa ccgtgcgcag gcagaccttg ttgtgccgcc ggaccaaacc
     4081 acggccagca aaccggtacg ccaccgcgaa tagcgacgcc atttttgaac ggtgtgttgt
     4141 tgctcaacca cagaacttct tcttcacccg caggtttcca cgagagaagg tgtgcgccct
     4201 gtaatgcaaa agaggctttt acctggggat gatcgaccac aatgaggtcc agttcatcca
     4261 gtttacgacg ggagaggaca ggggagattt gttcgatgac cggaagggca aaaattttct
     4321 taatcatgac gcagtccttt aacttcattt tatcaggtaa aaaaaagagc gaccgaagtc
     4381 gctcttttta gatcacagtg tcatctcaac ttatttggag atgtgagcga tcaggtccag
     4441 aactttgttg gagtaaccgg tttcgttgtc gtaccaggat accagtttca cgaagttgtc
     4501 gttcagagcg ataccagctt tagcatcgaa cacggaagtg caaacttcgc cgttgaaatc
     4561 ggtagatact acgtcatctt cggtgtagcc cagaacgcct ttcatttcgc cttcagcagc
     4621 agctttaacg gcagctttga tctgctcgta agttgcagct ttttccagac gaacggtcag
     4681 gtcaactacg gatacgttcg gggtcggaac gcggaacgcc ataccagtca gtttgccatt
     4741 cagttctggc agtactttac ctacagcttt agcagcaccg gtagaggacg ggatgatgtt
     4801 ctgggaagcg ccgcggccgc cgcgccagtc tttgtgagac gggccatcaa cggttttctg
     4861 agtagcggta gtagcgtgaa cggtggtcat cagaccttcg atgatgccga agttatcgtt
     4921 gataacttta gccagcggag ctaggcagtt ggtggtgcag gaagcgttgg aaacgatgtc
     4981 ctggccagca tatttgtcga agttagcgcc tttaacgaac atcggagtgt tgtctttaga
     5041 cggaccagtc ataaccactt tcttcgcacc agcggtgatg tgtttacgag cagtttcgtc
     5101 agtcaggaac agaccagttg cttcagcgac aacgtcaaca cccacttcgt cccatttcag
     5161 gttagccgga tcacgttcag cggtaacacg gattttttta ccgttaacga tcagatgacc
     5221 gtctttcact tcaacggtac cgtcgaaacg gccgtgagtg gagtcatatt tcagcatgta
     5281 tgccatgtaa tcagcgtcta acaggtcgtt gattgcaacg atctcgatgt cagaacgttt
     5341 ctgagcagca cggaaaacaa tgcgaccgat acggccaaaa ccgttgatac ctactttgat
     5401 agtcatatat tccaccagct atttgttagt gaataaaagg ttgcctgtaa aattacaaaa
     5461 accttacgca gcgtcaagcg gaatcgtgtc aatcattgcg acaaatcaat cctgtgccta
     5521 agcattacgc gactgactcg cctcactttt cctttgggct tgagaccaca tggggtcggc
     5581 gccccgaatt ttaaagggca attacgataa aaatgtgatt ttcatcacga tttaggtgca
     5641 gccatttcgt cgagatcaag tttaggacaa aattttgcca gttggtgtta atgttttgtt
     5701 agaatcagtc acgtaatgtg agcacgatta aagtgagatg tgagtaaatg gcgaataaac
     5761 cttcggcaga agaactgaaa aaaaatttgt ccgagatgca gttttacgtg acgcagaatc
     5821 atgggacaga acctccattt acgggtcgtt tactgcataa caagcgtgac ggcgtatatc
     5881 actgtttgat ctgcgatgcc ccgctgtttc attccgaaac caagtatgat tccggctgtg
     5941 gctggcccag tttctacgaa ccggtgagtg aagaatccat tcgttatatc aaagacttgt
     6001 cacatggaat gcagcgcata gaaattcgtt gcggtaactg tgatgcccat ctggggcatg
     6061 tctttcccga cgggccgcaa ccaacaggcg aacgttattg tgttaactct gcttctttac
     6121 gcttcactga tggcgaaaac ggcgaagaaa tcaacggttg aaaaaacgat tcagcaactt
     6181 attccacagg agcggaaaaa ttatgaatct tgatgacatt atcaacagca tgacgcctga
     6241 ggtataccag cgtttgtcga ccgccgttga actggggaaa tggcctgatg gcgttgcgtt
     6301 gaccgaggaa caaaaagaga attgcctgca actggtgatg ctgtggcaag cccgccataa
     6361 taccgaagca cagcatatga caattgacac aaatggtcag atggtcatga agagcaaaca
     6421 gcagttaaaa gaagattttg gtatctcagc aaagcctatt gccatgttta agtaagtggt
     6481 tcatcccctt tttgccgcgg ctatgaaggg gatagccaac aatgacccca cgactcgtct
     6541 caattatctc gacccgacct ggctaagtcc cagtccgatc tcttgcagtc gcgttcacgc
     6601 ctgaatgact tctcctgaac ttaagtctca gacctatttg gccggtaatc cctctcgaat
     6661 ttgctctttt tgcgtcccgc cttccaccaa cctgtaacag acccagccat tttctgcgta
     6721 attgtgatct gagttgtaaa atgcaggtca ttatctgtgt tgtgcagatt taatattgac
     6781 ctcaaaaatc atgtgggtaa gtatgatggc catctaagca aaacaaaaca cagaggatga
     6841 cgcaatgaaa gcactggctc ggtttggcaa ggcctttggc ggctacaaga tgattcatgt
     6901 cccacaaccc atttgtggcc cggaagacgt agtgattgaa attaaagccg cggcaatctg
     6961 cggcgcagac atgaagcact acaatgtcga tagcggttct gatgagttta actctatccg
     7021 cggccatgag ttcgcaggtt gtattgcgca ggttggtgaa aaagtcaaag actggaaagt
     7081 ggggcaacgc gtcgtgtcgg ataacagcgg tcacgtttgc ggcgtttgtc cggcctgtga
     7141 acagggtgat tttctgtgtt gtacagaaaa agtaaacctt ggtctggata acaatacctg
     7201 gggcggtggt ttttccaaat attgtctggt tcctggtgaa attctcaaaa ttcatcgtca
     7261 tgcgttgtgg gaaatccctg atggtgttga ttatgaggac gcagccgtac ttgaccctat
     7321 ctgtaatgcc tataaatcca tcgcgcagca atcgaaattc cttcctggtc aggatgttgt
     7381 cgtcattggc actggcccac tcgggctgtt ttccgtacaa atggcgcgga ttatgggggc
     7441 ggtaaatatc gtcgtcgttg gtctgcaaga agatgtggcg gtccgcttcc cggttgcaaa
     7501 agaactgggg gcgacggcag tagtaaatgg ttctaccgaa gatatggtgg cgcgctgcca
     7561 gcaaatttgt ggcaaagata atctgggact ggtgattgaa tgctccggtg ccaatatcgc
     7621 actgaaacaa gccatcgaaa tgcttcgccc gaatggggaa gtggtacgcg ttggaatggg
     7681 cttcaaacct cttgatttct cgattaatga cattaccgcc tggaataaaa gcatcattgg
     7741 gcatatggcc tatgactcca cctcatggcg taacgctatc cggctattag ccagcggcgc
     7801 tatcaaagtc aaaccgatga tcacgcatcg tatcggcctg tcgcaatggc gcgaagggtt
     7861 tgatgcgatg gtcgataaaa ccgcaatcaa agtgatcatg acttacgact ttgatgaata
     7921 actactctcc tgaaggaacg ttatcgcatg gaacagataa caaaaccgca ttgtggtgca
     7981 cggctggatc gcttaccgga ttgccgctgg cattcgtcaa tgtttgccat cgtcgcgttt
     8041 ggcttgcttg tctgctggag taatgccgtt ggtggcttga tcctcgcgca gctgaaagcg
     8101 ttgggctgga cagataattc cactacagcc acattctcag caatcacgac cgccggaatg
     8161 tttctcggtg cgctggtggg cggcatcatt ggcgacaaaa ccggtcgcag aaatgcgttc
     8221 atcctctatg aggccattca tattgcctcg atggttgtcg gtgctttctc accgaatatg
     8281 gattttctta ttgcctgccg ttttgtgatg gacgttgggc tgggagcttt actggttaca
     8341 ctgtttgctg gtttcaccga atatatgccc ggtagaaatc gcggaacgtg gtccagtcgg
     8401 gtttccttta ttggcaactg gtcatatccg ctctgctcac tgattgcgat gggactcacg
     8461 ccgctgatta gtgcagagtg gaactggcgg gtacaactgc ttattcctgc aatattgtcg
     8521 cttatcgcta cggcgctggc ctggcgctac tttcctgaat ccccgcgctg gctggaatcg
     8581 cgcggacggt atcaggaagc cgagaaagtc atgcggagta tagaagaagg cgtcatacgc
     8641 cagacgggta aacctttgcc gcctgtggtt attgctgatg acggtaaagc gccacaagcg
     8701 gtgccgtatt cagccttact gacaggagta ttactgaaac gcgtgatatt aggctcttgt
     8761 gtgctgatta ccatgaacgt tgtgcagtac acactaatta actggttgcc aacaatattc
     8821 atgacccagg ggattaattt aaaagactcg attgttttaa ataccatgag tatgtttggc
     8881 gcgccatttg gtatttttat tgccatgctg gtgatggata aaattccgcg taaaacaatg
     8941 ggtgtggggc tattaatcct gattgcggtg ctcggatata tctattcact gcaaaccagt
     9001 atgttgctca taacgctgat tggtttcttc ctgattactt tcgtctatat gtacgtttgc
     9061 tatgcctcgg cagtgtatgt ccctgaaatc tggccgacag aggccaaact ccgtggctcc
     9121 ggtctggcga atgcggtagg gcgaatcagt ggtattgccg caccttatgc cgttgcagtg
     9181 ctgctcagta gttatggtgt aacgggagtc tttattcttc tgggggctgt ttcaaatatt
     9241 gtcgcaattg ctatcgccac cattggaatt gaaaccaaag gtgtctccgt tgaaagttta
     9301 agtattgatg cagtagccaa taaataatac gggtggattc aaaatgaaaa attcaaaagc
     9361 aatattgcag gtgccgggca caatgaaaat tatttcagca gaaataccag tgcctaaaga
     9421 agatgaagtt ttgattaaag tagaatatgt cggtatttgt ggttcagatg tacatggttt
     9481 tgaatcaggc ccgtttattc cgcctaaaga cccaaatcaa gaaattggcc tgggtcatga
     9541 atgcgccggg acggttgtgg ctgtgggaag ccgcgtgcgc aaatttaaac cgggggatcg
     9601 ggtaaatatc gaacctggcg ttccttgcgg tcactgtcgt tactgtctgg aaggcaaata
     9661 taacatctgc ccggacgttg attttatggc gacacaaccc aactaccgcg gcgcattaac
     9721 gcactatctg tgtcatccgg agagctttac ttacaaactg cccgacaata tggacacgat
     9781 ggaaggggcg ctggtggagc ctgccgcagt cgggatgcat gccgcgatgc tggcagatgt
     9841 taaaccgggt aagaagataa ttattctggg agcaggttgt attggtttga tgacgttgca
     9901 agcgtgcaaa tgcctgggcg caacggaaat tgccgtcgtt gatgtactgg aaaaacgtct
     9961 ggcaatgggg gaacagcctg gtgcgacagt ggttattaat ggcgcaaaag aggacactat
    10021 tgcacgctgt cagcaattta ccgaagacat gggcgcagat attgttttcg aaacagcggg
    10081 ttctgcggtc accgttaaac aggcaccttt aatctggtaa tgcgcggcgg taaaattatg
    10141 attgttggta ctgtacccgg cgattcggca atcaatttcc tcaaaatcaa tcgcgaagtc
    10201 actatccaga cggtattccg ctatgccaat cgttatccgg tcacgattga agctatttct
    10261 tcagggcgat tcgatgtgaa atcgatggtg acgcatattt acgattatcg ggatgtacaa
    10321 caggcatttg aagagtcagt taacaacaaa tgcgacatta ttaaaggcgt tattaaaatt
    10381 agcgattaat taatacggga gaacagaaca tgctcgcaga tatcagatat tgggaaaacg
    10441 atgccacaaa taaatattat gcaattgccc attttaacgt atggaatgca gaaatgttga
    10501 tgggcgttaa agacgcagcc gaagaagcga aatccccggt tattatttct tttagtacag
    10561 gttttgtcgg taacacctca tttgaagatt tctctcacat gatggtatca atggcacaaa
    10621 aagcaacggt gccggtaata actcattggg atcatggtcg gagtattgag attattcata
    10681 acgcctggac tcatggtatg aattcattaa tgcgtgatgc ttctgcattt gatttcgaag
    10741 aaaatattcg tttaaccaaa gaggctgttg atttctttca tccgctgggt attccggtag
    10801 aggcggaatt agggcatgtc ggtaatgaaa ccgtttatga ggaagcgctg gcgggctatc
    10861 actataccga tcctgaccag gcggctgaat ttgttgaacg tacgggctgt gactcattgg
    10921 ctgtcgccat cggcaaccag catggggttt atacgtcaga gccgcaattg aactttgagg
    10981 tcgtcaaacg cgtacgcgat gctgtttctg ttccgctggt tttgcatggc gcatcgggga
    11041 tcagtgatgc cgacattaaa actgcaattt cgttgggtat tgcgaaaatc aacattcata
    11101 cggagctctg tcaggccgca atggtagcag tgaaagaaaa ccaggatcag cctttcctgc
    11161 atttagaacg tgaagtacgt aaagctgtaa aagaacgggc attggaaaaa attgaactgt
    11221 tcggttctga tggcaaagcg gaataatgat atggataatc tcgacgttat ttgtataggt
    11281 gccgctattg ttgatattcc attacaaccg gtcagtaaaa atatctttga tgtagattct
    11341 tatcctcttg aaagaatcgc aatgagcacc gggggcgatg caataaatga agcaacaatt
    11401 atttctcgtc tggggcatcg taccgcatta atgagccgca ttggtaaaga tgcctcaggg
    11461 caatttattc tcgaccattg tcgcaaagag aatattgata ttcaaagcct gaaacaggac
    11521 gtcagcatag atacatctat taacgttgga ctggtgacgg aggatggcga gcggacgttt
    11581 gtcacgaacc gtaacggcag tttgtggaaa ttaaatattg acgatgttga ttttgcgcgg
    11641 tttcctcagg cgaaattatt atcactggcc agtattttca acagtcctct attggatggt
    11701 aaagcgctaa cagaaatttt tacgcaagcg aaagcccggc agatgatcat ttgtgccgat
    11761 atgatcaaac cgcggttgaa tgaaacgctg gatgatattt gcgaagcatt gagttatgtc
    11821 gattatctgt ttccaaattt tgccgaggaa aaattactca cagggaaaga gacactggat
    11881 gaaattgctg actgctttct tgcgtgcggc gtaaaaacgg tggtgattaa aacgggtaaa
    11941 gacggctgct ttatcaagcg tggtgacatg acgatgaagg tgccggcggt cgcaggaata
    12001 accgccatcg acaccattgg cgcgggcgat aactttgctt caggttttat tgcggcactg
    12061 ttagaaggca aaaatctgcg tgaatgcgca cgctttgcca atgcaacggc ggctatctcg
    12121 gttctaagcg tcggtgccac caccggcgta aaaaacagaa agctggtcga acaattgctg
    12181 gaagaatacg aaggataatg aaggcaaatg aaaaagatac ctttaggcac gacggatata
    12241 acgctttcgc gaatggggtt ggggacatgg gccattggcg gcggtcctgc atggaatggc
    12301 gatctcgatc ggcaaatatg tattgatacg attcttgaag cccatcgttg tggcattaat
    12361 ctgattgata ctgcgctagg atataacttt ggcaatagtg aagttatcgt cggtcaggcg
    12421 ttaaaaaaac tgccccgtga acaggttgta gtagaaacca aatgcggcat tgtctgggaa
    12481 cgaaaaggaa gtttattcaa caaagttggc gatcggcagt tgtataaaaa cctttccccg
    12541 gaatctatcc gcgaagaggt agaagccagc ttgcaacgtc tgggtattga ttacatcgat
    12601 atctatatga cgcactggca gtcggtgccg ccatttttta cgccgatagc tgaaactgtc
    12661 gcagtgctta atgagttaaa ggctgaaggg aaaattcgcg ctataggcgc tgctaacgtc
    12721 gatgctgacc atatccgcga gtatctgcaa tatggtgaac tggatattat tcaggcgaaa
    12781 tacagtattc tcgaccgggc aatggaaaac gaactgctgc cgctatgtcg tgataatggc
    12841 attgtggttc aggtttattc cccgctagag cagggattgt tgaccggcac catcactcgt
    12901 gattacgttc cgggcggcgc tcgggcaaat aaagtctggt tccagcgtga aaacatgctg
    12961 aaagtgattg atatgcttga acagtggcag ccactttgtg ctcgttatca gtgcacaatt
    13021 cccactctgg cactggcgtg gatattaaaa cagagtgatt taatctccat tcttagtggg
    13081 gctactgcac cggaacaggt acgcgaaaat gtcgcggcac tgaatatcaa cttatcggat
    13141 gcagacgcaa cattgatgag ggaaatggca gaggccctgg agcgttaaat actcaacacg
    13201 attcacctct gtttctgtga aaactgtgat gaaaatctgt gattcaaata ggttattttg
    13261 aaagtaaaca tcggttatgc gataatcgcg ctaatgtgat gtaaaaagat acaggggtgt
    13321 aatcgtggcg gcaaaagaca ggattcaggc aattaagcaa atggttgcca acgataaaaa
    13381 ggtgacagtc tcaaatttga gtgggatttt tcaggtaacc gaagaaacca ttcgccgcga
    13441 tcttgagaag ctggaagatg aaggcttttt gaccagaacc tatggtggtg ctgttttaaa
    13501 tacagcggtg ttgacggaga atatccattt ttataagcgc gcttcatcgt tttatgaaga
    13561 gaagctgctt attgcacgca aggcactacc ctttatcgac aataaaacca ccatggcagc
    13621 cgattccagt agtaccgtaa tggaattgct caaattgtta caggaccgta gtgacctgac
    13681 gttgctaacc aactcggcgg aagcgattca tgttctggct cagtcagaaa ttaaagtcgt
    13741 ttcaacgggt ggggaactaa acaaaaatac actttccctg caaggaagaa ttactaaaga
    13801 gatcatcagc cgctaccatg tcgatatcat ggtaatgagt tgtaaaggtc ttgatattaa
    13861 cagcggcgcg ctggactcta atgaagcgga agctgaaatc aaaaagacaa tgatccgtca
    13921 ggcgacagaa gttgcgttat tggttgatca ctctaagttt gatcgtaaag cttttgtcca
    13981 gttagctgac tttagtcata ttaattatat aataactgat aaatcaccgg gtgcagagtg
    14041 gatagcattt tgcaaagaca ataatatcca gctggtgtgg taatttaagc acacgttgtc
    14101 aatattattt gttcctgcga ctggaaataa aatgatgata aagatgcggc aagtgaattt
    14161 gccgcaattg ctacctttta tagcaaatcc gaggcaccca atggaacaat atgatcaaat
    14221 tggcgcaaga ctggaccgct tgcctttggc ccggtttcat tatcgtatat ttggtataat
    14281 aagctttagt ctgttattaa cggggttttt gagttactca ggtaatgtcg tcttagcaaa
    14341 gctggtaagc aatggatggt caaataattt cctctatgcc gcctttacct cggcattaat
    14401 gtttggttat ttcatcggct cacttactgg tgggtttatt ggtgactact ttgggcggcg
    14461 cagggcgttt cgcataaatc ttctcatcgt cggtattgct gcaacagggg ccgcttttgt
    14521 ccctgatatg tactggctca ttttctttcg cttcctgatg ggaacaggaa tgggggcgct
    14581 gattatggtt ggctatgcct catttacgga gtttatcccc gcgacggtgc gtggaaaatg
    14641 gtccgcgcgg ctctcatttg ttggtaactg gtcgcccatg ctgtctgcgg cgataggcgt
    14701 ggtggttatc gcttttttta gttggcgaat aatgtttctt ctgggtggta ttggcatact
    14761 gttagcctgg tttctctcag gtaaatactt tatcgagtcg ccacgatggc tggaagggaa
    14821 agggcaaatc gccggtgcag aaagccaact tcgtgaagta gagcagcaaa ttgaaaggga
    14881 gaagagtatt cgtttacccc agcttacttt gaaccagagc aacagcaagg ttaaggtaat
    14941 caagggtacc ttcaggctcc tgtttaaagg ggaaatgtta cgacgtacat tagttgcgat
    15001 aactgtttta attgcaatga acatttcgct ttataccatt accgtatgga taccgaccat
    15061 atttgttaac tccggcattg atgtcgataa atcaatatta atgaccgctg ttattatgat
    15121 tggtgctccg gtaggaatat ttattgcagc attaattatt gatcattttc ctcgtcgatt
    15181 atttggctcc gccttactta ttattattgc cgtgttaggc tatatctatt caagtcagac
    15241 tacagagtgg gcgattttaa tctatggtct ggtgatgatc ttctttttat acatgtatgt
    15301 ttgcttcgcg tcggcggttt atatcccgga gctttggcca acgcatttac gcctgcgcgg
    15361 ttcgggtttc gttaatgccg tcggacggat cgtcgcagtt ttcacgccct atggcgttgc
    15421 ggcattatta acacattatg ggtcgatcac ggtgtttatg gtgcttggtg tcatgttagt
    15481 gctctgtgcg ctggttctct ccatttttgg catcgaaacg cggaaggtgt cgctggaaga
    15541 gatttctgag gtgaattaaa tctttcgttg atggcatcaa ttgccagatg taccactgac
    15601 gtgttttatt tggcctacaa tgccagtaca gaattgtagg ccagcgtaaa attacccctg
    15661 cgtctcttcc cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa
    15721 cgcgtgtgca ctgtcctggg gctggatatt cacgccacga cagccatcgg taatcacgtt
    15781 taccttataa cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc
    15841 caggcccata acgatcaatt catcgatttc atgatcgcgc aaccagtcat caagcgcggt
    15901 tttctgccga cgtccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc
    15961 tttatggaac accgctgcga tctctttttg attcagtaac ggatgtaatt gtgcgccttc
    16021 actgttctgc acacagtgtt caggccagaa agtttgccgc aggccgccga gttggcctgg
    16081 cgtataaggc tctatgccgt gctggctggc aaagctgccg tgattcgccg gatgccagtc
    16141 ctggctggcg ataaccgctt ctccgcgcga ctggcaccag tcaataaggc ggttagcaac
    16201 ctccaccgta ctgtcacctt ccggtacggc gagcgcgcca ccagcacaga aatcattttg
    16261 taaatcgacc agtaacaggg cgcgatgggg cattgcagtc tccttaatca tccggcgtca
    16321 gttcgccgcg caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc
    16381 tcagcaggta atgcagtttg gttagcgtgg cttctacagt catatctgca ccgtcaataa
    16441 cgccggcatg ggcgagggcg ttaccggtgg cataaccact catattcact ttaccggaca
    16501 tacattgtgt caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca
    16561 ggaaggcttt gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca
    16621 ccggttggcg cagaaaattg cgcacgacgt cagcagaaat ccctggataa atcgtcacca
    16681 cgccaattgg ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg
    16741 gtggcgtatt caaacgacga atatggatac cagcttccag taacggagga aggtttggag
    16801 aggcaaacgc atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc
    16861 ggttattgaa aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat
    16921 tcagcagatt aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca
    16981 caatgaccgg tttaccgaga ttctcgagca tgaacgacag cgcagaggcg gtatacgcca
    17041 tcgtgtcggt accgtgtaga atgacaaaac catcatagtc gtcatagtgc gctttaatat
    17101 cttcagcaat atgctgccag tcttccggcg tcatatcaga agaatccatc aacggcgtat
    17161 attcatgaat ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt
    17221 ggcgttgtag atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg
    17281 tcccgcccgt gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat
    17341 cgaaagagca aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag
    17401 gaaatatggg gcgagacagc gggaagagga ttcgtcgagg taaaaaataa tgtggccgac
    17461 gatctgtcgg ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca
    17521 taacggtttt gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag
    17581 gctaccgagg caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg
    17641 acagaaccag acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat
    17701 tccagatgcc actgtttcac ttttgcgagc tctgctgctt tggcaaccgc atcatcgaaa
    17761 tccccgagac tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc
    17821 tgggcgattt tatcaatttg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata
    17881 aagcgtttat agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt
    17941 gccctggtga tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca
    18001 atcgaatcca gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc
    18061 agggtgctgg ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat
    18121 gccgccatgc cgcccatcga tacaaccaca gacttacccg ctgcccgggc tgctgccagt
    18181 tcagcgcgaa tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca
    18241 atcgctttca ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg
    18301 ccaacattcc cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg
    18361 atgctgtcac cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga
    18421 taatttttat cagtcttact ccagccgaac tctttggtca gagctttttc gatttcggca
    18481 ctcgatgcca gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg
    18541 gttttggtta aaccctcaag caacccttgc gcgccaggga atacctgctg agcagggatc
    18601 tgccggttag cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg
    18661 tcagcttcgc gggctgccgg tgacatatca tcacgaataa acggttcaac ggcagattta
    18721 tacgtaccca cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag
    18781 tacagaccgt tggtagcaaa accgtgcaga tcaaccacgc cttgcggtga cagccaaatt
    18841 ttattggcga aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa
    18901 accggtttcc cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc
    18961 tggtcgccgc ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gcggtcgtcc
    19021 ttcgcctggc gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa
    19081 ctggcaccaa gcagctggcg gcttaattta ctaaaccgct gagaactgtc tggtttatcg
    19141 acgatcacac cagaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca
    19201 ccaccactga cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg
    19261 ttaagtacca tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca
    19321 ataaatcgcc aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg
    19381 caatagatgt ggttatcgta atgacccaac cggcacttgt cagcaggaat tgcggactgt
    19441 gctgtaacaa aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat
    19501 caaatggatg cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc
    19561 gcgccaacgg gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat
    19621 aagtccatgc aaccgtggca tttttttgtg attgaagggg aagggtgcga gcgtttcagc
    19681 gccgtactgg aacagggggc gattgctgcc ggtagtgatg ataaagctat cgacaaagcc
    19741 cgtaatgcgc cgttccgcgc accgctcatc atcacggtgg tggcgaaatg caaagaaaat
    19801 cataaagtcc cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa
    19861 atggcagccg ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt
    19921 ccggtagtgc gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac
    19981 ctcggcacgc cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt
    20041 gtaacttatt tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat
    20101 tcagacactc tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac
    20161 aggagatgtc catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt
    20221 gcggctgtaa aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt
    20281 ttgttgatct gaatttgctt gtgggtaatg aaacccgcga cgatgcggcg gtgtacgatc
    20341 tgggcaatgg caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc
    20401 ctttcgattt tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg
    20461 gcaaaccgat tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa
    20521 ttgcccgcga agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg
    20581 ctggcggtca ctccatcgat gcgccggagc cgatttttgg tctggcggta acggggatcg
    20641 taccgaccga gcgggtgaag aaaaacagta ccgcacaagc cggatgcaaa ctgttcctga
    20701 cgaaaccgct ggggatcggc gttcttacca cggctgagaa aaaatcactg ttgaaaccag
    20761 aacatcaggg actggcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg
    20821 ccaacatcga aggcgtaaaa gcgatgaccg acgttacggg ctttggtctg ctgggccact
    20881 tgagcgaaat gtgtcagggg gctggtgtgc aggcacgcgt cgactatgaa gcgatcccga
    20941 aactccccgg tgttgaagag tacattaagt tgggcgcagt acctggcggc actgaacgta
    21001 actttgccag ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatctgctgt
    21061 gcgatccgca aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg
    21121 ttaaaactac agccgccgag tttggcattg aactgacggc gattggcgaa ctggtgccag
    21181 cgcgcggcgg tcgtgccatg gttgagatcc gttaatttaa tgcggttgtt tattgccgaa
    21241 aaaccgagtc tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat
    21301 ggctttatcg agtgcggtaa tggtcaggtg gtgacttggt gtatcggtca cctgcttgag
    21361 caggcgcagc cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg
    21421 attgtcccgg aaaagtggca attacagcca cgaccctccg tgaccaaaca acttaacgtc
    21481 atcaaacgct tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa
    21541 gggcaattgc tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag
    21601 caggtgcagc gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgatcgac
    21661 cgtcttcgtt ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt
    21721 gccgactggc tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc
    21781 ggttatcagg gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg
    21841 cgccgcgatg aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat
    21901 atcgtgacac ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa
    21961 ccgtaccagg atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac
    22021 cgcattagtg gtcaaccggc tattgtcacc agctataacg ataaacggga atcagaatcc
    22081 gcgccgctgc cgttttcgct ttcggcgttg cagattgaag cggcaaaacg cttcggtctg
    22141 agcgcgcaga acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact
    22201 tatccgcgtt ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg
    22261 atgaatgcca tcagcgttca tgcaccggat ctgttgccgc agccagtggt agatccagat
    22321 atacgcaacc gctgttggga tgacaaaaag gtcgatgcgc accacgccat catcccgacc
    22381 gcacggagtt ctgcgatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc
    22441 cgtcaatacc tgatgcagtt ctgcccggat gcggtgttcc gcaagtgtgt tatcgaactg
    22501 gacattgcca aaggcaaatt tgtcgctaaa gcccgttttc ttgctgaagc aggctggcgc
    22561 acgctgttag gcagcaaaga gcgcgatgaa gaaaacgacg gcacgccatt gcctgtggtg
    22621 gcgaaaggcg atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg
    22681 ccgcgccatt ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg
    22741 caggacaaag atctgaaaaa ggtccttcgt gcgaccgatg gtctggggac agaagcaacc
    22801 cgtgccggga ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat
    22861 atccactcca ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga
    22921 ccggacatga ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc
    22981 tatcaggact ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa
    23041 cgtacgccgg tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat
    23101 aagaaaaagg ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagcc
    23161 ggaagcgggg cgatagcgta agcgagcgaa tctttcgtgc tattcgagtc atattctgaa
    23221 atatccagcg gatcaagaaa attcgttgga tatttttttg catggataaa attatcgcct
    23281 ctaaagtatg taataacagg gaatgtgatg aaaaaagttt tattgcaaaa ccatcctggg
    23341 agcgagaagt attcttttaa tggctgggga atatttaata gtaattttga acgggtgatt
    23401 aaagaaaata aggccatgct gctttgtaag tgggggtttt atttaacatg tgttgtcgct
    23461 gtaatgtttg tattcgcagc gataacatcc aacggtttga atgaaagagg cctgattacc
    23521 gcgggatgct cttttcttta tctattaatt atgatggggc ttattgttcg ggccggtttt
    23581 aaagcaaaaa aagaacaact gcattattat caggctaaag gtattgagcc gctcagtatc
    23641 gagaagttac aggcgctaca attgatcgca ccttatcgat tctatcataa gcaatggtct
    23701 gaaacgctag agttctggcc gcgaaagcct gaacctggca aagatacctt ccaatatcat
    23761 gtgcttcctt ttgactcgat cgatatcatc agtaaaagac gcgagtcttt agagaatcaa
    23821 tggggtatcg aagatagcga aagttattgt gccttaatgg agcattttct ttctggcgac
    23881 catggagcca atacctttaa agcaaacatg gaggaagccc cagagcaggt tatcgccttg
    23941 ttgaataaat ttgctgtttt tccctcagac tatatctctg attgcgctaa taatcgctcc
    24001 ggtaaatcct cggcgaagct aatatgggcg gcggaattat catggatgat ctcgatatca
    24061 agcacagctt ttcaaaacgg gacaattgaa gaagaactgg cctggcatta tataatgctt
    24121 gcttctcgaa aggcgcacga gttgttcgaa agcgaagaag attatcaaaa aaatagtcta
    24181 atgggatttc tttactggca tatctgctgc tatcgcagaa agttaacgga tgctgaactt
    24241 gaagcgtgtt atcgttacga caagcagttt tgggagcact acagtaagaa atgccgttgg
    24301 cccataagaa atgttccctg gggagcatca tccgttaaat actcataaac gcctgaaatt
    24361 ttgccggggg cgctgcgctt gaccggccta cattagagcg taactgcaat gaattgtgcc
    24421 catctgtagg cctgataagc gtagcgccat caggcattta caacttaaat cacaccctgc
    24481 gccagcatcg catcggcaac cttcacaaaa ccggcaatgt tcgcgccctg cacgtagttg
    24541 gtttgctcac cttcaccacc atgctcaaca caggcatggt ggatatccag catgatgtga
    24601 tgcaaacgtg cgtcaacttt ctcggctttc cagcccaggc gcgcagcgtt ttgtgccatt
    24661 tccaggcccg acgttgctac accgccagca ttagccgctt tacccggtgc aaacagtacg
    24721 cctgcctgct ggaacagttc agtcgcttcg atggtggtcg gcatatttgc cccttcagcg
    24781 acggctttaa cgccattggc gataagctga tgcgcggcgt caacatccag ttcgttctgg
    24841 gtggcgcaag gcagggcgat atcaaccggt acagaccacg gctgttggcc ttcgagatag
    24901 accagaccaa attctttggc gtaatctgcc actcgaccat cgcggctggc tttgatttcg
    24961 ataagacgtg ccagtttctc tttcgtgaat ccgctttcat caactacagt gccgctggag
    25021 tctgacgcag tgatcacacg agcaccaaat tccatcgctt tttcgatagc gtactgggcg
    25081 acgttgccgg agccagaaac ggaaacgctc atcccttcaa aacccatacc gtggcgtttt
    25141 aacattgctt ctgtgaaata aaccagaccg tagccggtag cttccgggcg aataagacta
    25201 ccgccaaatg aaaggccctt accggtgaag acgcaggcgg tattgttgga gagctttttc
    25261 atcatccccg ccataaagcc gacttcacga ccaccaacgc cgatatcacc tgccggaacg
    25321 tcggtatccg cgcccaggtg gcgatacagt tcagtcatca gcgtctggca aaaacgcatc
    25381 actttacctt cgctttttcc tttcggatcg aaatcgctgc cgcctttacc accgcccatc
    25441 ggcagggtag tcagggcatt tttgaaggtt tgttcaaagc cgaggaattt gagaatggaa
    25501 aggttaactg acggatggaa gcgcataccg cctttgtacg ggccgatggc agagctgaac
    25561 tgcacacgcc atgcacggtt gacctgtatc tggttgcgat catcaaccca taccacgcga
    25621 aactggatca cgcgctccgg ttcaaccaga cgctccagta atgacatctg gcgatatttt
    25681 ggattttgtt caagaaaagg ccagagtgtg gtcattactt cacgaacggc ttgcgcgaac
    25741 tcggtttgat tcgggtcgcg cttttggaca tggttgagga atgactccag agaatatgtc
    25801 tgatccatag atataaaaac cttatatatt aatacgattg tgcttatgtt gtttatgtca
    25861 tgtgcttttg cagttttcga ctataccatc aagaatgtga cggtaagcaa gaaaaaaatg
    25921 acccaggaaa gcaaattaat aacgagagta atctcataac caaatgcgat aactcagacg
    25981 ctaaagtttt gccacccggc taccgttatc agtagcagag acaataacgt taaggagttc
    26041 cctgtgagtc gagcattgtt cgccgtagta ttagcatttc cattaattgc actggctaac
    26101 ccgcattatc gtccggacgt ggaggtaaac gtaccgccag aagtgttcag ttcaggcggg
    26161 cagtcagcgc agccctgtac tcagtgctgc gtttatcagg atcaaaatta ttctgaaggg
    26221 gcggtaatta aagccgaagg aatactgctg caatgccagc gtgatgacaa aacccttagc
    26281 actaatccgc tgctctggcg gcgcgtaaag ccataaacgc ctctaataat ggaatgtcag
    26341 caggggccag cggatattgc agcgcctctt caggtgagca ccagaccagc gcctgatgtt
    26401 catgtgcctg taacgtcccg tggaagtcgg gtacgtgcca ggcatgaaga tggataatcc
    26461 ggcccgaaac ttctcgctga tggctggcaa catactcacc cacagttgct tcgatgtcca
    26521 attcttcgtt taactcacgc accagcacct gctgctggct ttcatccagc tcgactttac
    26581 ctccggcaaa ctcccataat cccgcctgat cgctctgggc ggggcgttgc gcgagtaaaa
    26641 ttttgccatc acgttcaatg atggcggcaa caacttcaat cattttcata aattacacac
    26701 gggcagataa gcgaaaaatg ccataatatc cgcactgttg ctcaatcaga aaggctttat
    26761 atttaaggag tgcctgtgct agaccgccat cttcatcccc ggattaaacc gttgttgcat
    26821 cagtgcgtgc gggttcttga taaaccgggc attacgccag atggtttaac attagtggga
    26881 tttgccatcg gcgtgctggc tctgccgttt ctggcgctgg gctggtatct ggcggcgcta
    26941 gtcgtcattt tgttgaacag gctgcttgat ggtctggacg gtgcgctggc gcggcgcaga
    27001 gggcttaccg acgcgggcgg ctttcttgat atttctctcg attttctctt ttacgcgctg
    27061 gtgccgtttg gctttattct tgctgcaccg gagcaaaatg cgctggcagg cggctggttg
    27121 ttgtttgcgt ttatcggcac gggcagcagt tttctcgcct ttgctgcact ggcagcaaaa
    27181 catcagattg ataaccccgg ttatgcacat aagtcgtttt actatctggg gggattaacc
    27241 gaaggcacag aaacgatctt actgtttgtg ctgggatgtt tattccctgc atggtttgcg
    27301 tggtttgcgt ggatcttcgg tgcgttgtgc tggatgacga cctttacgcg ggtgtggagt
    27361 gggtatctga cgctgaagtc gctccagcgt cagtaattgc aatgttattt gctactgccc
    27421 gggccgcgtt caccggttgc aaccggattt tttggatcgc tgctccattc gtaccagccg
    27481 ccgtcataca cggaaacatt cttccaaccc atggcgcgtg cgtacataaa ggtttcggac
    27541 gcgcgccagc cggtgccgca gtagaatgaa acttgctgat ctggtttgat attccatgct
    27601 ttccacatag cggtaatatc atcggcgcta cgcatggtgc catccgggtt atagaaatct
    27661 tccatatgcg tcgagtcgct accagcgtgt ccccaacgtg ctccggctat ttcacctttt
    27721 ggtttaatat agctgtaacc gctggtcgta ccgataaatt ctggccacga acgaatgctc
    27781 accagcgatg catcctggcg atgcagcagt ccacgggcct gttccatatc gagcatcaac
    27841 tgcggttgtg ccgggatctt cacgccgaaa tccggttccg ctttcacttt cggtggcgtt
    27901 cctcgctcaa caggcagtcc cgcgtcggac caggtttgcc agccgccatc cagcagacgc
    27961 acatctttca cgcctgcgta gagcataatt tgcgccacac gcgctccagc gtatacgtca
    28021 cgcccgtaca gaatgaccgt ggtgtcatgg cgaatgccgt gttttgccag catcgctttt
    28081 agttgttcat cagaaacttt gttccacagc ggttcgcttt ccacttcgtt ggtatcgatg
    28141 tagtcagcgc caggaatatg gctgataagg taaagcttag gagcgcccta ggccgcttca
    28201 atgactttcc agtcactgac aggtttcgcc gtaacctctt taccttgttg caggtcatgc
    28261 agccattgcg gataaaccag ctgctcaaag tgcggcagtt tttgtagacg ggaaggttcg
    28321 cttagcgcgt cactcaggat ggagatatgc gtaaaacctg ctttttgcag tcgcgttttg
    28381 acggcgtcga catctttgcc attaccgtac agcgccaccg gggcatcggc tttcaggtta
    28441 tgttgcttga tccacgcgtt gagctgttcg gtgctcattt tgtcgagcca gctagcagag
    28501 aggtttaagg cggcaggttc atgaccagaa gggccattta aggtttgtgg ccagccgtta
    28561 taaaacgcgc tggggcgagt atctatcgct ttgccatttt gttgctgaag ttggtcaagt
    28621 gtaagaggct tcgccagttc agcggcccac gaagaagcgc aagccagccc taaagccatt
    28681 gccagcgcgg tcatttgaga aacacgtttc atcgaaaatc ccgacattgt agttttgagt
    28741 gcgccattct gaactgacgc attgagaaaa actttgcgtt atcgcagttt gttgtaattt
    28801 tctgaccact gcgccatatc cagaacagaa ctatcagcag gaacatcctg gagatcgtgc
    28861 gttacctgaa cgacggggat cgccagggcg cgaacttcgc tgaacaccca ctggcgaaaa
    28921 ttatcgcgca gagccacatc aagacggctg aatggctcat ccaggagtaa cgcttttggc
    28981 tgggcgagaa gggcgcgtag tagagcaacg cgcgctcgct gaccgccaga caaagtggca
    29041 ggatcctgat ggaaagctcc gtcaaggcct gaacgctcaa gtgcatcatt cacggcatta
    29101 cgtcgggcat tccccttaag tgtcgccggt agcgccagca gtaaattttg cccgacgctg
    29161 aactggtcaa ataacagtgc atcctgaaaa agaatgccaa tctgacgctg tgcggtgggt
    29221 agcatgtcaa tccgttgctc attgagccat agctcacctg tacaagaaaa ctgtccggcc
    29281 agtgcaccaa tcatccatga aaacagagtg gattttccac agccagacgg ccccattaac
    29341 gtgacgatgt cacctttatc aaccgtaaag ttaacgtttg tcagcaagcg gctttctggt
    29401 aaacgtagcg aaacattttt cacgcagagc attagcggat tccttgtctg acataaccta
    29461 cccattttgc gactaacgcg gtcagggcaa aaataataag cggtaacaac agttgccata
    29521 acgcctgggc ggcgagaata ccgttgctgc cgccgctgct taatgccacc gcctcagtgg
    29581 tgagcgtcgg aaaacgcccc gcgcccagcc acagcgtcgg catatactgc gcaatactga
    29641 ctgaaaatcc caccgcgaag gcagtcagca cagggcgcaa catgagtggg catttcacgt
    29701 aaaagaagat tttggcccgc gaccagccca gtgtttgcgc aatcaaaatt aaccgtgaat
    29761 caatgcgctg ccaggcgggt tgcaggataa acagcatcca cggcattacc cacagcagat
    29821 gcccccagac caccgccgtc cagcttccat ccaggttcag ccatagcgcc agcgtgtact
    29881 ggcccgccac cagtggcaga gcgggcaata gaatgggcaa ccatagccac aactggcgac
    29941 gctgtgcccc ccattccagc cacagcagta acaggagcaa agcgatgaat gttgctacca
    30001 gccccattgt cagactgttg atgagtgctt cactattgat cgtcgactga tccgcgagga
    30061 tcgccagcag aaccacacac agcacaccgg ttaagggtaa aaaactcgcc agcgtagtgc
    30121 ctggtaataa aggtgtggca ggcttgcgaa tgccatctac gcggggaata gtgcgccgcc
    30181 agctgcgcca aaacgtaggc ggcgagtaac agcatcagca gcagactggc gagtgcgcct
    30241 ttcgtttgtt gatccgcgtc gccctgagtt aaccactgcc agctaattac tgccagcgtc
    30301 ggtggattac ctggcccaag aataatcgcc acatccacga ccgaaagcga ccaggcaaca
    30361 atcgccagca tcgccattgc cagcgaaggc gcgacggagg gcaacagcag ccagttcagg
    30421 cattgccagc ggctgtagcc cagcgaatcc agcacaatga cctgctgcaa cagccgtttt
    30481 tcgctcaata ctgccactaa gatccacagc agaaatgcgc tttctttcac tgcaagggtg
    30541 aggcccagac cgatccaaat cggtccattg gcggagtgaa atacgggaaa tagtcatata
    30601 gcagccctcc gtcagcaaag agcagcaggg cgctggtggc aaaagccaca tggggaatgg
    30661 cgagcagcca cggcagacgg gcgcacatac gctgccattt cggcccaggc catagcgcca
    30721 caatcaccag cagggcaatc aacaatgccc cgaccgccgc gatggttgtc gacaccagcg
    30781 ttgccagtaa tgcctgcggt aactgtggat cggcaaataa cgcctgccag cgtgtcaacg
    30841 acaacgcagg cgatatcagc gtgagagctg ccgggatcag cggtgcataa atcaccgcca
    30901 ccatcgccca cagcaaaaag attaatgcgt accgtaacgg cgtaaccatt cttgttccag
    30961 tgcattcacc catcctgcgt gcggttcagc cagtaccggc ggcagatcct gcggcattct
    31021 tgattgcaat atttcgcgct gcccgtcagg cagtttttgc ggatcgagaa cagaaggatc
    31081 gccccagaca acgggatctg ctttacgcag ttgcgcatcg ggtgagagca ggaaattggc
    31141 aactaccttc gccgcagcac tggcattcgc gttggctgga gtggtgacga aatgcacgtt
    31201 gccaatcatc ccctcgcgaa agccaaaact gtaactgctt gccggcaaat cgccgctggc
    31261 gattttttgc tgcgcatgtg cggggttaaa ggtcagcgac aggcgcaatg tgccggcttt
    31321 cagcagagca tccatccgcg cgggcgaagg cgggaaatcc tttccttcgc gccacaaata
    31381 cggatgcagt gcatcaagat attgctacaa gggcgcagtg acacgggcga aagtcgcatc
    31441 gtccggcgct tcttttaatg cggcgggatc tggcgtcagc ataatcagca actgttcaag
    31501 aaacgccgtg ccggtaaagt ccggtgggcg cggataagta accgtgccgg gattggcttt
    31561 agcaaactcc agtaaggctt gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc
    31621 gataaacgtc agttgtgcgc cgccccaggg cgattccgca ccttgtgtcg gcactgaaaa
    31681 atctccccgc accggcagct gtgtgtcgac atagcgccag ttgggcagag tctccgccca
    31741 gcccgtttgt aataaattgg cctcttt
//
LOCUS       DAECNO010000043        31187 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.043, whole genome shotgun sequence.
ACCESSION   DAECNO010000043 DAECNO010000000
VERSION     DAECNO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.043"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            389..2047
                     /locus_tag="KKO08_002001"
     CDS             389..2047
                     /locus_tag="KKO08_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBD6318250.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="KKO08_002002"
     CDS             complement(2044..3000)
                     /locus_tag="KKO08_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBD6318251.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="KKO08_002003"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="KKO08_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBD6318252.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="KKO08_002004"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="KKO08_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBD6318253.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="KKO08_002005"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="KKO08_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBD6318254.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="KKO08_002006"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="KKO08_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBD6318255.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="KKO08_002007"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="KKO08_002007"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBD6318256.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="KKO08_002008"
     tRNA            complement(9859..9946)
                     /locus_tag="KKO08_002008"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="KKO08_002009"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="KKO08_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBD6318257.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="KKO08_002010"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="KKO08_002010"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBD6318258.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="KKO08_002011"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="KKO08_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBD6318259.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="KKO08_002012"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="KKO08_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBD6318260.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="KKO08_002013"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="KKO08_002013"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBD6318261.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="KKO08_002014"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="KKO08_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBD6318262.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="KKO08_002015"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="KKO08_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBD6318263.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="KKO08_002016"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="KKO08_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBD6318264.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="KKO08_002017"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="KKO08_002017"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBD6318265.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="KKO08_002018"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="KKO08_002018"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBD6318266.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27413
                     /gene="dmsA"
                     /locus_tag="KKO08_002019"
                     /pseudo
     CDS             24969..27413
                     /gene="dmsA"
                     /locus_tag="KKO08_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27424..28041
                     /gene="dmsB"
                     /locus_tag="KKO08_002020"
     CDS             27424..28041
                     /gene="dmsB"
                     /locus_tag="KKO08_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBD6318267.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28043..28834
                     /locus_tag="KKO08_002021"
     CDS             28043..28834
                     /locus_tag="KKO08_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBD6318268.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28870..29496)
                     /locus_tag="KKO08_002022"
     CDS             complement(28870..29496)
                     /locus_tag="KKO08_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD6318269.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29811..30959
                     /locus_tag="KKO08_002023"
     CDS             29811..30959
                     /locus_tag="KKO08_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6318270.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7686 a         7643 c         8379 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 gcgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ttgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct agtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatgg cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgtg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgatcgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgatcg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctaccgat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc
    25261 tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc tacaatccgg
    25321 accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca
    25381 ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt
    25441 acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc
    25501 gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc
    25561 ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac catacctacg
    25621 gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt
    25681 ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac
    25741 aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat accgacaccg
    25801 gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta
    25861 acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc ctcgataaat
    25921 attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat ggtcattata
    25981 aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc
    26041 aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca
    26101 aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa
    26161 cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca
    26221 gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc
    26281 cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa
    26341 tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga
    26401 tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg
    26461 aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct
    26521 catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact
    26581 ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta
    26641 aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg
    26701 gcgttgagca acagtttact gaaggccgta cctaggaaga gtggatgcgg catctgtatg
    26761 cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga
    26821 tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc
    26881 cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg
    26941 ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct
    27001 acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta
    27061 caggtttcca ctataaatct cgcgttcact caacttacag caacgttgat gtgctgaaag
    27121 cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca
    27181 acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga
    27241 cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat gacccggatg
    27301 caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc
    27361 tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa
    27421 ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc
    27481 tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt ccgccgcatt
    27541 tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt
    27601 gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc
    27661 ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc
    27721 attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg
    27781 aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg
    27841 atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    27901 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    27961 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    28021 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    28081 cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa
    28141 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt
    28201 actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt
    28261 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    28321 cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    28381 attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat
    28441 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    28501 gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt
    28561 gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc
    28621 agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc
    28681 cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc
    28741 cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt
    28801 gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc
    28861 ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc
    28921 aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg
    28981 cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc
    29041 ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac
    29101 atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac
    29161 accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt
    29221 atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt
    29281 cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg
    29341 taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg
    29401 ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc
    29461 atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt
    29521 ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    29581 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    29641 aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat
    29701 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    29761 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    29821 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg
    29881 tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag
    29941 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    30001 tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg
    30061 gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg
    30121 cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga
    30181 cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt
    30241 ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg
    30301 ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    30361 aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc
    30421 ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc
    30481 ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac
    30541 tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc
    30601 gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    30661 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    30721 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    30781 aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt agcgctatgc
    30841 taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct
    30901 atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa
    30961 tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct
    31021 gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt
    31081 tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc
    31141 ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcagg
//
LOCUS       DAECNO010000044        29634 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.044, whole genome shotgun sequence.
ACCESSION   DAECNO010000044 DAECNO010000000
VERSION     DAECNO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.044"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..186)
                     /locus_tag="KKO08_002024"
     CDS             complement(<1..186)
                     /locus_tag="KKO08_002024"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBD6318271.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="KKO08_002025"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="KKO08_002025"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBD6318272.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="KKO08_002026"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="KKO08_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBD6318273.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="KKO08_002027"
     CDS             3863..4144
                     /locus_tag="KKO08_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBD6318274.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4437..4961
                     /locus_tag="KKO08_002028"
     CDS             4437..4961
                     /locus_tag="KKO08_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6318275.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5042..5716
                     /locus_tag="KKO08_002029"
     CDS             5042..5716
                     /locus_tag="KKO08_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD6318276.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5732..8212
                     /locus_tag="KKO08_002030"
                     /pseudo
     CDS             5732..8212
                     /locus_tag="KKO08_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8228..9261
                     /locus_tag="KKO08_002031"
                     /pseudo
     CDS             8228..9261
                     /locus_tag="KKO08_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9343..9681)
                     /gene="rcnB"
                     /locus_tag="KKO08_002032"
     CDS             complement(9343..9681)
                     /gene="rcnB"
                     /locus_tag="KKO08_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBD6318277.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9900..10748)
                     /gene="rcnA"
                     /locus_tag="KKO08_002033"
                     /pseudo
     CDS             complement(9900..10748)
                     /gene="rcnA"
                     /locus_tag="KKO08_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10870..11142
                     /gene="rcnR"
                     /locus_tag="KKO08_002034"
     CDS             10870..11142
                     /gene="rcnR"
                     /locus_tag="KKO08_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBD6318278.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11234..11330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11365..12153
                     /gene="thiM"
                     /locus_tag="KKO08_002035"
     CDS             11365..12153
                     /gene="thiM"
                     /locus_tag="KKO08_002035"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBD6318279.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12150..12950
                     /gene="thiD"
                     /locus_tag="KKO08_002036"
     CDS             12150..12950
                     /gene="thiD"
                     /locus_tag="KKO08_002036"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBD6318280.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13015..13836
                     /locus_tag="KKO08_002037"
     CDS             13015..13836
                     /locus_tag="KKO08_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBD6318281.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13884..14630
                     /locus_tag="KKO08_002038"
     CDS             13884..14630
                     /locus_tag="KKO08_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD6318282.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGGLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14604..15569)
                     /locus_tag="KKO08_002039"
     CDS             complement(14604..15569)
                     /locus_tag="KKO08_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBD6318283.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15566..16569)
                     /locus_tag="KKO08_002040"
                     /pseudo
     CDS             complement(15566..16569)
                     /locus_tag="KKO08_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16566..17843)
                     /locus_tag="KKO08_002041"
     CDS             complement(16566..17843)
                     /locus_tag="KKO08_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6318284.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18100..19152
                     /gene="fbaB"
                     /locus_tag="KKO08_002042"
     CDS             18100..19152
                     /gene="fbaB"
                     /locus_tag="KKO08_002042"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBD6318285.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19460..20314
                     /gene="gatY"
                     /locus_tag="KKO08_002043"
     CDS             19460..20314
                     /gene="gatY"
                     /locus_tag="KKO08_002043"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBD6318286.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20343..21605
                     /gene="gatZ"
                     /locus_tag="KKO08_002044"
     CDS             20343..21605
                     /gene="gatZ"
                     /locus_tag="KKO08_002044"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBD6318287.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21615..22067
                     /gene="gatA"
                     /locus_tag="KKO08_002045"
     CDS             21615..22067
                     /gene="gatA"
                     /locus_tag="KKO08_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBD6318288.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22098..22382
                     /gene="gatB"
                     /locus_tag="KKO08_002046"
     CDS             22098..22382
                     /gene="gatB"
                     /locus_tag="KKO08_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBD6318289.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22386..23741
                     /locus_tag="KKO08_002047"
     CDS             22386..23741
                     /locus_tag="KKO08_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBD6318290.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23789..24829
                     /gene="gatD"
                     /locus_tag="KKO08_002048"
     CDS             23789..24829
                     /gene="gatD"
                     /locus_tag="KKO08_002048"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBD6318291.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24935..25708
                     /locus_tag="KKO08_002049"
     CDS             24935..25708
                     /locus_tag="KKO08_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6318292.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25790..26689)
                     /gene="yegS"
                     /locus_tag="KKO08_002050"
     CDS             complement(25790..26689)
                     /gene="yegS"
                     /locus_tag="KKO08_002050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBD6318293.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27095..27412
                     /locus_tag="KKO08_002051"
     CDS             27095..27412
                     /locus_tag="KKO08_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318294.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27754..29115)
                     /gene="yegQ"
                     /locus_tag="KKO08_002052"
     CDS             complement(27754..29115)
                     /gene="yegQ"
                     /locus_tag="KKO08_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBD6318295.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29262..29593)
                     /locus_tag="KKO08_002053"
                     /pseudo
     CDS             complement(29262..29593)
                     /locus_tag="KKO08_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7699 a         6878 c         7480 g         7577 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctgta attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aaatcattta tataaatgat
     4201 ttcgtctttt ttattgatat caacaatacc atttacataa atctacatta attaatagat
     4261 gtgataaata tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt
     4321 tttaaatcaa taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt
     4381 ttccaagaga tacagactct taacaaccaa aaataaattt tattaaggaa catattatga
     4441 aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg
     4501 ctgcagacgt tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta
     4561 aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct
     4621 ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt
     4681 taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca
     4741 aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg
     4801 atgtcctaga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
     4861 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
     4921 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
     4981 ctccaggtaa taaacatggc tgctatcccc atggcggctt tttaatttaa gaggcattaa
     5041 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
     5101 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
     5161 gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
     5221 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
     5281 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
     5341 agaaagcatt ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg
     5401 caagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
     5461 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg
     5521 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     5581 tgttaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat
     5641 caaaagtaat aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat
     5701 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     5761 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     5821 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     5881 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     5941 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     6001 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     6061 tgttcagggt gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc
     6121 gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     6181 tattaatgcg ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc
     6241 gggtaacaac aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca
     6301 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     6361 taccctgtat ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat
     6421 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     6481 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     6541 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc
     6601 tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     6661 tagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc
     6721 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga
     6781 aggggcgagc aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt
     6841 attgacgctg tatggcggca cgatggttgc taataattac tatgcgttta ccctctgaac
     6901 gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca
     6961 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     7021 ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac
     7081 atttaacgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat
     7141 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa
     7201 tatgagccag tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga
     7261 ttactggggg cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     7321 gcggataagc tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa
     7381 acgttttaat atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg
     7441 tcggcaaata tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa
     7501 tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag
     7561 tcatcaacat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     7621 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt
     7681 ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac
     7741 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     7801 cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca
     7861 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     7921 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     7981 ctggtttata aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa
     8041 tgatatccat ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac
     8101 caatgaaata ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     8161 aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg
     8221 tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag
     8281 ctatgcggca tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt
     8341 tgcacttact caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt
     8401 gagtacaaat actatcaccc ctatgagccc atcggatatt attgttggac tttataacga
     8461 taccattaaa ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa
     8521 taatcagagc agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc
     8581 aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt
     8641 atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt
     8701 aggtggcaag tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc
     8761 aggtgtctat tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca
     8821 ggacgattta ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa
     8881 caaagcaaca ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg
     8941 tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac
     9001 caacagcaac acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt
     9061 aatggttcgc cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta
     9121 aaagaaattg cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc
     9181 agttactacg tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta
     9241 atcaacgtga aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata
     9301 acaaggtgtc tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat
     9361 ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc
     9421 acccatatag gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg
     9481 cttaatgttg tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg
     9541 cttaaagtca gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga
     9601 acccgcggtg gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa
     9661 catcttattt ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata
     9721 tctttcctgt catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg
     9781 taaaaagtgt aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga
     9841 aatagctgat ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt
     9901 tatcgcatta tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac
     9961 agactggaaa aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg
    10021 acctgctgaa cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca
    10081 atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc
    10141 aacagcacgg taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa
    10201 atttgccagt tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct
    10261 cgtgcatggg catcctgata ctcgccatat tcgtgatggt gatgatgtcc ttggtcgtgg
    10321 tgatgttcgt ggtcttgatg atgttcgtga tcgtgatgat ggtgatgatg ctcataatca
    10381 tgcccgtgca tattctccag ccagttgcgt tcgccgcgct aggtacgcca gaacatccag
    10441 aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga
    10501 gcagtaaagc gcttactgat caccatcccg ccaaaggcaa tcaaccagac caccgcggta
    10561 tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg
    10621 atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca
    10681 agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat
    10741 tcggtcatga taataattct tagtattaat tcggcaatct gattctactc ccccccagta
    10801 cctgatacta cccccccagt agattaatag tgctatgatt tttcatattc ttgttaacca
    10861 ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag
    10921 attcagggcc aggtcgtggc gctcaagaaa atgctcgacg agccgcacga atgcgctgca
    10981 gttttacaac agattgctgc tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt
    11041 aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat
    11101 ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta
    11161 aaactgccgt ttttcctcgt ttacaacggg ggcgctggac attaccatcc tcctctgcga
    11221 tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg
    11281 tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc
    11341 ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg
    11401 cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg
    11461 gtgcaaacct ttaccgccaa taccttgctg gcgctcggtg catcgccagc gatggttatc
    11521 gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc
    11581 acactgacgc agccacgcgc acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct
    11641 caaacacgct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt
    11701 tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg
    11761 gcattagctg gcattgctaa tggcggacgg ggagtggata ccactgacgc cgcagctaac
    11821 gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc
    11881 gaggtggatt atgttaccga tggacatcgt atcattggta ttcacggtgg cgatccgtta
    11941 atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg
    12001 ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga
    12061 gaacgcgcag tcgccagaag cgaggggcca ggcagttttg ttccacattt ccttgatgcg
    12121 ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg
    12181 gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg
    12241 gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt
    12301 cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg
    12361 tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg
    12421 cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa
    12481 aaagcggcga cccgctgctt tcaccttcgg cggttgctac gctgcgcagt cgattattgc
    12541 cacaggtttc attaataacg ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac
    12601 acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg
    12661 gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta
    12721 cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggca
    12781 ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg
    12841 acaccgtaca ggaggcaaaa atctggcttt catcggcgtt agcccaggcc gacacgctgg
    12901 aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact
    12961 atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag
    13021 ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc
    13081 atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt
    13141 acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgcattcct
    13201 gccagttaca ccattcacgg attagatgtt tcgcgctggc aggagcgtat cgactggcag
    13261 cgggtggcaa aaatgcgcga caacgatatc cgcttacagt ttgcttttat taaggctacg
    13321 gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat
    13381 ggcctgctgc gcggggcgta tcattatttc tccccgtcgg tatccgcttc agttcaggcg
    13441 cgattatttc tgcaaacggt ggatttctca caaggcgatt tccctgccgt gctggacgta
    13501 gacgaacggg gaaaattatc ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa
    13561 atggtcgaaa aacgcacggg aaaaaagccg attatttact caggagccgt tttttatcac
    13621 accaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta ttatcaacgt
    13681 cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg tggacaggta
    13741 gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggggat gagttgcagg
    13801 cattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata ctcccaccag
    13861 actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt gcccaactga
    13921 acgagcgtat tttagcggcg gataacacgc cgctttatat taagtttgcc gaaacggtaa
    13981 aaaatgccgt gcgcagcggg gggctggagc atggcaatat tttgcccggt gagcgtgatt
    14041 taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag
    14101 aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac atcttcgaat
    14161 actcgttgaa agaagcgaga ggtttttctc agcaggtggt attgtgcggg aaaaagcccg
    14221 atacgctatg ggttaataag cgtgtcgtga aatgtcccga agaagttgcg cagcagctgg
    14281 cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag
    14341 cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt gatgccatcg
    14401 gaatttctct ttatgactat ttccgcagcc agcacattta cccacaacgt acgcgttccc
    14461 gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta gatagcaaaa
    14521 taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct attgagtaca
    14581 gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc
    14641 gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc attgcccagt
    14701 aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc acctgcatga
    14761 ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat gcagccagaa
    14821 gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa cggcgcggca
    14881 aatttctcct gccattgctt gccaagtgtt gttatctctg cggataaagc aaaacgttcg
    14941 gcggcaatct cagcctcttg acgattgagt gacactaaag gtcgacaggc catgatccgc
    15001 gccagtaatg cgtccgggat atcgccaata cgtgggcaaa aatcgataaa cggcgtcacg
    15061 tcttgcagct tttccagcca ttccactaac aattcgccgc agggcgaggc cagttgataa
    15121 ccggaaaaat agagcaggtt gccaggcgca acggttaatc gtgccaacca ttggcgattc
    15181 cattgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg ctcaaccagt
    15241 gccagacacc agccgttatc gctttcggcg ttatcgatca ggctgattaa cccctctttc
    15301 gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa ggcattaccc
    15361 gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc gccaacgtta
    15421 acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata agcgtcggcg
    15481 atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt taattcaggc
    15541 agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga tatttcgcca
    15601 gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagctcc gcctttaacg
    15661 caggatcgac agcgttaatg ccatgcaacg cgccgcaaat cgccgtcgcc atagcaccta
    15721 tggtgtctgt gtcgccgcca agattggcgc acaggacggc gcagcggttt gggtcggttt
    15781 gtgccagttc aactagcgca atggcgcacg gaacggactc aatagtgctg gtgcctgcgc
    15841 caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcatta cgcacaattt
    15901 tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa cgtggtggtg cgtttttgct
    15961 gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc tgaccagcgt tctccgtcaa
    16021 tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga tcggatttat
    16081 gggtcgggct ggacgccagc gccacatcat caataaagga atcaacatca cgcgctggaa
    16141 gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggttacgccg ttgttttcca
    16201 gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta
    16261 gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc gagaatatta cgcccaatca
    16321 gattcggatc gatcttgcct tcacattcca gtaacgcatc cgccagacac aggccatcga
    16381 ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc gcgttattct cctttggtcc
    16441 aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg cggctgcgtg gccaaagctc
    16501 ggagggcatc cccatcgcat cccctaacgc ctgcccataa agagcaccga gaatacgttc
    16561 tgttttcatt taacttcccc ttgtgtcaac gcaatatctc gatcatcgac cttgatagcc
    16621 gtaatttcgt tgtcggattc gcggaaaaaa atcatgaaca gcacggcgat aatggcaatc
    16681 atcaccgcgc cgaaagtcca catcccggac cagttgaaag tcagtccgtt taccggttcc
    16741 tgataagcga acatcttttc catcatcaca ccgccaagac gatagccgag caaactgccg
    16801 aagccctggc agcagagcgt gatcagcccc tgcgcagcgg tacgcatatg cacgggggct
    16861 tttttatcga catagatgta agcggtaacg tagtaaaaat cgtaacttac gccgtgaagc
    16921 aaaataccga ggaacagtaa cgcgtaggtg aaatattcat ccgcactacc gtaaataaag
    16981 aagccatagc agatcgcagc ggtgaccaga ccaagcaata ataccttttt gataccaaag
    17041 cgtttagtga aaaacggcaa tgccagcata aagaagattt cagagaactg gccgagcgtc
    17101 atccagccgg tggcgttttt catgccaact tcggtcagat aaccgttggc aaagatgtaa
    17161 tagaacgcca gtggcatcgc aaacaggaat gaacagaaga aaaagacgag gaagttttta
    17221 tcgcgcagca ggatcagcgc atccaggccg agcatgactt taatgtccat tttgccggtg
    17281 ctttttggcg gcgtgtcggg caggaaaaac gcaaacacac cgagcagagc agaacttccg
    17341 gcggtaatca gcagcgggat gttagtcggt gagatatcgg cataccccag catttgcggc
    17401 aagaaaccac atgccagacc ggaggcaatc cagccgatag tgcccatcac acgaatgcgt
    17461 gggaaatcac gctccacatc cggcacgttg gcaaaagcga tgctgttagt cagcgcaatg
    17521 gtcggcatat aggttagcga gtaggccagc agtaacggaa agaacccggc aaaagtggtc
    17581 tgttgcgcag cgaaatacat cagcacagca ccagcgaaca tcaataccgc cagcactttc
    17641 tgcgccgaga aaaagcggtc agtgatggag ccaaccagaa ttggcgacag gatcgccgca
    17701 atggctgtgc aggcatacga ccagccaatt tctccggcac taaaaccgct tttacttaac
    17761 cacaaccaca atggcacaaa ccacgcgccc cagataaacc attcaacaaa catcatgaac
    17821 gacagctttg ctgttgtttt catcttttaa ttccttcatg acaggtaatg taaggtacgc
    17881 gatggcaata ccacataata atgccttttg aatacctttg cggtgaaaac ttgaccgcgc
    17941 taacattttt tctgatgaat cgagccaaca gaaaacgctg aaaaaacatc caaaagatgg
    18001 aaaaactcgc ccgtaacgac taggattatg attgcccgca aaaggcgggc caggacaatc
    18061 catagccgat atccaatcgg aatttacggg agcatagtaa tgacagatat tgcgcagttg
    18121 cttggcaaag acgccgacaa ccttttacag caccgttgta tgactattcc ttctgaccag
    18181 ctttatctcc ccggacatga ctacgtagac cgcgtgatga ttgacaataa tcgcccgcca
    18241 gcggtgttac gtaatatgca gacgttgtac aacaccgggc gtctggctgg cacaggatat
    18301 ctttctattc tgccggttga ccagggcgtt gagcactctg ccggagcttc atttgctgct
    18361 aacccgctct actttgaccc gaaaaacatt gttgaactgg cgatcgaagc gggctgtaac
    18421 tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc ggcgctatgc gcatcgcatt
    18481 ccatttctcg tcaaacttaa tcacaacgag acgctaagtt acccgaatac ctacgatcaa
    18541 acgctgtatg ccagcgtgga gcaggccttc aacatgggcg cggtggcggt tggtgcgact
    18601 atctattttg gctcggaaga gtcacgtcgc cagattgaag aaatttctgc ggcctttgaa
    18661 cgcgcgcacg agctgggcat ggtgacagtg ctgtgggcct atttgcgaaa ctccgccttt
    18721 aagaaagatg gcgttgatta ccatgtttcc gccgacctga ctggtcaggc gaaccatctg
    18781 gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg cggaaaataa cggcggctat
    18841 aaagcgatta attacggtta taccgacgat cgcgtgtaca gcaagttaac cagcgaaaac
    18901 ccgattgatc tggtgcgtta tcagttagct aactgctata tgggccgggc tgggttgata
    18961 aactccggcg gtgctgcagg cggtgaaact gacctcagcg atgcagtgcg tactgcggtt
    19021 atcaacaaac gcgcaggcgg aatggggctg attcttggac gtaaggcgtt caagaaatcg
    19081 atggctgacg gcgtgaaact gattaatgcc gtgcaggacg tttatctcga tagcaaaatt
    19141 actattgcct gatacgcctc atacgaacag caccaccccg taggacggat aagacgttta
    19201 cactgcatcc ggcaacctat gcctgatgcg acgcggaagc gtcttatcat gcctacatag
    19261 cactgccacg tatgtttaca ccgcatccgg cataaaaaca cgcgcacttt gctacggctt
    19321 ccctatcggg aggccgtttt tttgcctttc actcctcgaa caattttcat attgttgttt
    19381 ttgtgatcat tatctcgaca tttaaaagca aataatttca ttatattttg aaatcgaaag
    19441 caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag cagatgctga acaacgcaca
    19501 gcgcggcggt tatgcggttc cggcattcaa tattcacaat ctcgaaacga tgcaagtggt
    19561 ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc ctggcacatt
    19621 tactcatgct ggtacagaaa atctgttggc gctggtcagc gcgatggcga agcaatatca
    19681 ccatccactg gcaattcatc tcgaccatca cacgaaattt gacgatatcg ctcagaaggt
    19741 tcgttctggc gtgcgctcag tcatgattga cgcctcgcat ttgccttttg cgcaaaatat
    19801 ttcacgggtc aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg tcgaggcgga
    19861 gctggggcaa cttggcggcc aggaagatga tgtgcaagtc aatgaagtcg atgcgttgta
    19921 caccaacccc gcacaggcgc gtgaatttgc cgaggcaacc gggattgatt ccctggcggt
    19981 cgccatcggc acggctcacg gtatgtatgc cagcgcaccg gtgcttgatt tttctcgact
    20041 ggagaacatt cgccagtggg tgaacttacc gctggtgctg catggcgcgt caggattatc
    20101 gactaaggat attcagcaaa ccatcaaact ggggatatgc aaaatcaacg ttgcaacgga
    20161 gctgaaaaat gccttcttgc agtcgttaaa aaattacctg accgagcacc ccgaagcgac
    20221 cgatccccgg gattatttgc agtcggctaa atccgcaatg cgcgatgtgg tgagcaaagt
    20281 gattgccgat tgtggctgcg agggcagggc ataacgcacc tgccatttaa caaggaaaaa
    20341 acatgaaaac gttaattgcc cggcataaag ctggtgaaca tatcggcata tgttcagtct
    20401 gttctgccca tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac agcacgcgca
    20461 aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat accggaatga
    20521 caccggcaga ctttcgcgaa tttgttttta cgattgccga taaagttggg tttgcacgcg
    20581 aacgcattat tctcggcggc gatcatctgg gaccaaactg ctggcagcaa gaaaatgcgg
    20641 atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata tgttcgtgct ggcttcagta
    20701 aaattcatct tgatgcgtca atgtcctgcg cgggggatac cataccgtta gcaccagaaa
    20761 cggttgcgga acgagctgct gtgctttgct ttgctgcgga aagtgtggcg acagattgcc
    20821 agcgtgagca actgagctat gtcattggca ctgaagttcc ggttccgggc ggtgaggcca
    20881 gcgccattca gtcagtacac atcacccatg ttgaagatgc cgccaatact ttacgtacgc
    20941 atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt aacacgtgtg attgccatcg
    21001 tggtgcagcc gggtgtggaa tttgatcaca gcaatattat ccattatcag ccgcaggaag
    21061 cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt ttatgaagca cattctaccg
    21121 attaccagac ccggacggct tattgggaat tagtccgcga tcactttgca atattgaaag
    21181 tcggtcccgc attaaccttt gctttacgcg aggcgatatt tgcactggca caaattgagc
    21241 aggaacttat cgcccccgaa aatcgcagcg gttgcctggc ggtaattgaa gaagtgatgc
    21301 tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg ttttaacgat tcattactgg
    21361 atattcgtta cagcctgtcg gatcgtattc gttattactg gccgcatagt cggattaaaa
    21421 atagcgttga aacgatgatg gtgaatctgc aaggcgtgga catcccactg ggcatgatta
    21481 gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg ggaattatca gcaatgccgc
    21541 atcagcttat tatgaataaa atttatgatg ttttgcgcgc ctatcgctac ggctgtgcgg
    21601 aataaggacg gtatatgact aacctgtttg ttcgtagcgg aatttctttt gtcgatcgta
    21661 gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa aggtgtggtt catgatacct
    21721 ggccacaggc attaattgcc agagaagcag aattccctac cgggataatg cttgagcagc
    21781 acgccattgc aataccgcat tgtgaggcga ttcatgctaa gtcgtcagcc atttatctgt
    21841 taaggccaac aaataaagtt cattttcagc aagcggatga tgataacgac gtggcggtat
    21901 cgttggttat tgcgttgatt gtggaaaatc cgcagcagca attgaaactt ttacgctgtt
    21961 tatttggcaa gttacaacag cccgatatcg tcgagacact aattactctt cctgaaactc
    22021 agttaaagga atacttcaca aagtatgttt tagattcaga cgaataaatc cctctgtaac
    22081 aataaaaagt actatttatg aaacgcaaga ttattgtcgc ttgcggaggc gcggttgcga
    22141 cctctacgat ggcggcggaa gaaattaaag agttgtgtca gagtcataat attcctgttg
    22201 aattaatcca gtgtcgggtt aatgaaatag aaacctatat ggatggtgtg catttgatat
    22261 gtaccactgc cagggtggat cgtagttttg gcgatattcc gttagttcac ggcatgcctt
    22321 ttgtttctgg tgtcggtatc gaagcattac aaaataaaat tctgactatc ttacaggggt
    22381 gacctatgtt ttcagaagtc atgcgttata ttctcgacct cggccctaca gtgatgctgc
    22441 cggttgtcat cattattttt tctaaaatat taggcatgaa ggcaggcgat tgctttaaag
    22501 cgggtctgca tatcgggatt ggctttgttg gcattggcct tgtgattggc ttaatgctgg
    22561 attccattgg tccggcggcg aaagcgatgg cggaaaattt cgacctgaat ctgcatgtgg
    22621 tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc gtcgcaaatt gcgctggtgg
    22681 cgattccgat tgcgatattg gttaacgtgg cgatgttact gacccgtatg acgcgggtgg
    22741 taaatgttga tatctggaat atctggcata tgaccttcac cggcgcgttg ctgcacctgg
    22801 caaccggttc atggatgata gggatggcgg gtgtggtaat tcacgcggcg tttgtttata
    22861 agctctgcga ctggtttgcc cgcgataccc gaaatttctt tgagctggaa ggtattgcta
    22921 ttccgcacgg tacgtcggcg tatatggggc cgattgcggt gctggtcgat gctatcatcg
    22981 agaaaatccc aggcgttaac cgaattaaat ttagcgccga cgatattcag cgcaaatttg
    23041 gcccttttgg cgaacctgtc accgtgggtt ttgtgatggg gctgattatc ggcatcctcg
    23101 cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa aacggcggca gttatgctgc
    23161 tgatgccacg gatgattaaa cccatcatgg atggtttaac gcccatcgct aagcaggccc
    23221 gtagtcgttt acaggcgaag ttcggcggtc aggagttcct gattggcctg gatcccgcat
    23281 tactgctggg gcatacggcg gtggtatcgg caagcctgat ttttattccg ctcaccattt
    23341 taattgctgt ttgtgttccg ggtaatcagg tactgccgtt tggcgatctt gccactatcg
    23401 gcttcttcgt ggcgatggcg gtcgcggtgc atcgtggaaa tctgttccgc accttaatct
    23461 cgggtgtcat cattatgagc atcaccctgt ggatcgcgac gcaaactatt ggtttgcaca
    23521 cccaaccggc ggctaatgct ggggcgttaa aagccggggg tatggtggct tcaatggatc
    23581 agggcggttc tcccattacc tggttactga ttcaggtttt ctccccgcaa aatattcccg
    23641 gtttcattat tatcggcgca atttatctga ccggtatttt catgacctgg cgtagagcgc
    23701 gtggttttac taaacaagag aaagccgttc tcgcagaata atttttacct gagggggagg
    23761 ttatccccct taacaatcag gagtttttat gaaatcagta gtgaatgata ctgatggtat
    23821 cgtgcgcgtt gcaaaaagcg tcattcctga aattaaacat caggatgagg tgcgggtaaa
    23881 aattgccagc tcgggcttat gtggttccga tttacccagg atatttaaaa atggtgcaca
    23941 ttattatcca ataacgttag gccatgaatt tagcggctat attgatgcgg tgggatccgg
    24001 tgttgatgat ttacaccctg gcgatgcggt tgcctgtgtg ccgttattac cctgttttac
    24061 ttgtccagag tgtttgaaag ggttttattc ccagtgcgca aaatatgatt ttattggctc
    24121 gcggcgtgat ggtggatttg ctgaatatat tgtcgttaag cgaaaaaatg tctttgctct
    24181 acccacggat atgcctattg aggatggggc ttttattgag ccgattaccg ttggcctgca
    24241 tgcttttcat ttagcgcaag gttgtgagaa taaaaacgtt attattattg gtgccggaac
    24301 cattggcctg ctggctattc agtgcgctgt cgcgctggga gcaaagagtg tgacggcgat
    24361 cgacattagc tcagaaaaac tggcactggc aaaatctttc ggtgcgatgc aaacatttaa
    24421 cagtagcgaa atgagcgcgc cgcaaatgca gagcgtttta cgcgaactgc gctttaatca
    24481 gcttatcctc gagacggctg gcgtaccgca aactgtcgaa ctggcggtag agattgccgg
    24541 tcctcatgcc caactggcgc tggtgggcac gttgcatcag gatctgcatt taacatcggc
    24601 aacgtttggc aaaatattgc gtaaagagct gacggttatc ggcagttgga tgaactattc
    24661 cagcccttgg ccggggcagg agtgggaaac ggcgagccgg ttgctgacag aacgtaagtt
    24721 aagcctggag ccattaatcg ctcaccgtgg aagctttgaa agcttcaccc aggcggtgcg
    24781 tgacatcgct cgtaatgcca tgccgggcaa agtgttgctc attccatgaa accgcgggcc
    24841 agcgtgatgc tggcccggta ttgtgcaaaa cagatcattc accaatggtc ccccttcgtt
    24901 tacactagcc acaattgaat atgggtaaat gacgatgaat tcattcgagc gaaggaataa
    24961 gatcatccaa ttagtgaatg aacagggaac cgtgcttgtt caggatctgg cgggagtatt
    25021 tgctgcctcg gaagcgacaa tccgtgccga tttgcgcttt ctcgaacaaa aaggcgtggt
    25081 tacgcgcttt catggcggtg cggcgaaaat aatgtccggt aatagtgaaa ccgagaccca
    25141 ggaagtcggg tttaaagagc gatttcagct cgccagcgcg ccaaaaaaca gaatagcgca
    25201 ggcggcagtc aaaatgatcc atgaagggat gaccgttatt ctcgacagcg gaagtaccac
    25261 aatgcttatc gccgaaggat taatgaccgc taaaaatatc acggtgatta ccaacagtct
    25321 cccggcagcg tttgcccttt ccgaaaataa agacattact ctggtcgtct gtggtggcac
    25381 ggtccgccat aaaacgcgct cgatgcatgg ttccattgcc gagcgttcat tgcaggatat
    25441 taatgccgat ttaatgtttg tcggtgctga tggtattgat gcggttaatg gtattacgac
    25501 ctttaatgaa ggttattcga ttagcggggc gatggtcacc gccgcaaata aagtgattgc
    25561 tgttctcgat tcatcgaaat ttaatcgtcg cggttttaat caggtactgc caatcgaaaa
    25621 aattgacatt attgttaccg atgatgctgt gtcagaggtg gataaattgg cattgcagaa
    25681 gacacgggta aaattaatta cggtgtagta ttgttctgta tgccggataa ggtgtttaca
    25741 ccgcatccgg cattacggat tctccctggt tcaagagaga atagtttgat taacgcaata
    25801 atggacaatc tggtggtaat cgacaacgca acgccgccgg aagtatttca atatggaagt
    25861 tttgcccact caacggttcg ccatcaagat taaaagtgat ttcgtgtggg gcttgtatat
    25921 caaaccacga cgaagcgcct tcgataatat tcgggttatc ttcgtcagat tttaaggttg
    25981 atacgagagc cggaagaatt tcatcgccgg taaaaatgcg cagttgcagc aagccatcgt
    26041 taattaacgc gttcgggcac aattgttgac cgccaccggc ctgacgcccg ttaccaatac
    26101 caatgaccag ggcgtcacct tgccagtgaa agttttcacc gcggatttca caacggtccg
    26161 gttgcagagt atccatgcgc attaagccat gaatgatgta agagacgcca cccagcgcgg
    26221 cttttaattt ttccggcgtt tctgtggtaa tacgcgtccc aaatccgcct gtcgccatat
    26281 taataaaaca ggtttgtttg ttgacctgcg ccatatctat cgcaatggca ttaccggcaa
    26341 ttgccagttt cagcgcctta tccagtgcct caggaatccc tacactggtg gcaaaatcat
    26401 tggcggttcc taatggcaaa attcccagcg cgggtatgtc atccccctca cactgaatca
    26461 acgccgtaga aacttcatta atggtgccat cgccaccacc ggcaatcacc gttgcgacgc
    26521 ccaacttccg ggcctcctct acaaatcgtg cggcatcacc tttctcccag gtgacccgca
    26581 catggatcgt cattccttcc tcacgcaaca gcataattgc ttcgcgcaag ggtagattgt
    26641 cagtactttt accattaaga atcagtaagc tggcgggaaa ttctgccatg atcgtatgtg
    26701 cctttatgat tggtctgtag atagtgtaga gcagaaaaca aaaggtggag tcagaacagg
    26761 atgaaagtcg gaggataaaa agtcagttca agtatggagg attcagtggt taccaccagg
    26821 agcttgcaat ggggacagcg aatactaacg gctgccaccg ataaatttca aaaaagagca
    26881 tatacctaat attcaactaa acagtggcat cttcaatata atatattaaa gcccccatgg
    26941 agttaccctg aagggcctca atgtccgtaa ttcctactta tgtaggaaat gttgtacaga
    27001 gcatttatta taatcctatt caattataat aatcatgcca ttattatatt taaacactag
    27061 agagtgtcgt tggtatttaa tgggggaagg tgagatgaaa aagatagctg ctatatcatt
    27121 aattagtatt tttcttatat ctggttgtgc tgtgcataat gatgaaacaa gtatcggtaa
    27181 atttggtctt gcatataaaa gtaatattca gcgtaaactc gataaccaat actacaccga
    27241 agccgaagct tctttagcca ggggcagaat atctggtgca gaaaatatag taaaaaatga
    27301 tgcagcccat ttctgtgtta ctcagggcaa aaaaatgcag atagttgacc tgaagacaga
    27361 aggtgcagga ttacatggcg tcgctcgtct gacattcaaa tgtggagagt gagaatattt
    27421 ttttgtaagc gtcaaacatg cgcgttctgg ctgtgcgtag ccggaacctg ggcgagcacg
    27481 ataccgttac gtgaaaggca ttgtgctatg aagggggatt ctatcgatgt ggtcaatgga
    27541 agacggttat cagggatcaa tccttcttta cagcttatgt acaaaaaata agcccgtgta
    27601 agggagatta cacaggctaa ggaggtggtt cctggtacag ctagcatttt atggattatg
    27661 tttttcagcg aaacggatga taaccttaat aaatgcagct gtatgtgatc ggtttctaag
    27721 aattttccat ccgggaaaaa taatcgaaat taatcactta ccgtgggggt tacgcgtggt
    27781 ttccccggag aaattacgca ttagcagcgc gtaatttagc tcaagatcct gcgggaccgg
    27841 gagccacaca gtataaccat cgcctggtgc gaccggcata gcttcgcctt tggcgttttc
    27901 catgtgctca agggtaaagt taatgttgcc ttgcggcgtc atcagctcaa gactgtcgcc
    27961 aacggagaat ttatttttca ccgctaccgc tgcgaggtcc cccttgcgct caccggtaaa
    28021 ctcaccaaca aactgctggc ggtcagaaac tgaataaccg tattcgtagt tctgataatc
    28081 gtcgtgagta tgacgacgca ggaaaccttc ggtatagcca cgatgcgcca gaccttccag
    28141 agtttccagc aggctggtat cgaacggttt tcccgcagcg gcgtcatcga tagctttgcg
    28201 gtaaacctgt gcggtgcgtg cgcaatagta gaaagatttg gtacgacctt cgattttcag
    28261 cgaatgcacg cccattttgg tcaggcgttc tacatgggcg atggcgcgca gatctttcga
    28321 gttcatgatg taagtgccgt gctcatcttc aaacgcggtc atatactcgc ccggacgctg
    28381 ggcttcttcg atcataaaca ctttgtcggt tggcgcgccg atacccagcg tcggctcaac
    28441 attttgcacc ggaatcggct cgtacttgtg tacgatgttg ccaacgtcat cttctttccc
    28501 ttcctggacg ttgtactccc agcggcaggc gttggtgcag gtgccctggt tcgggtcgcg
    28561 cttgttgata tagccagaga gcaggcagcg accggagtag gccatgcaca gtgcgccgtg
    28621 aacgaagatc tcgatctcca tatccggcac ctgattatgg atctcttcaa tctcttccag
    28681 cgacagctcg cgagagagga tcacgcgggt cagtcccatt tgctgccaga atttcaccgt
    28741 cgcccagttc acggcgttag cctgcaccga gaggtggatc ggcatttcag ggaagtgctc
    28801 acgcaccagc ataatcagcc ctggatcgga cataatcagc gcatccggcc ccatttccac
    28861 caccggtttc aggtcacgga taaaggtttt cagcttggcg ttgtgcggtg caatgttgac
    28921 cacgacataa aactttttcc ccagcgcgtg ggcttcattg atgccgagct gaagattttc
    28981 gtggttgaat tcgttgttgc gcacacgtag ggagtaacgc ggctggcccg cataaacagc
    29041 atctgcgcca taagcgaaag cgtaacgcat atttttcagc gttcccgccg gggaaaggag
    29101 ttccggttta aacataattc tctcgttctg atgacaggtc agatccgctt cacctgatga
    29161 agcggttagg ggaggccccc actttaaggg cgggcattgt agcgctgtag gggggatctg
    29221 gtaaagcgcc ggacgccaac aacccacgcc cggcatcgta attacgtatt gtctttcacc
    29281 gtctggcttg tgccattggc tttaacggag gcaattccgg tttcacgaga ttgcgcggtg
    29341 gcgtacatct ggctggagcc gataatttga tgattagcgg ctttcagatt gaaatagaat
    29401 ttgccgttgc ttgccgtttt ttctcatagc gctcttcttg cgggctgttg ctacgcaccg
    29461 acgcgatgcc cttttccgca gaggcttttg aggtatacag ctcgctggtg aggatagtct
    29521 caccattgcc cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa
    29581 accaaccagc cataatgact ccttgattga aattgcttat cgaaaacagc atct
//
LOCUS       DAECNO010000045        29236 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.045, whole genome shotgun sequence.
ACCESSION   DAECNO010000045 DAECNO010000000
VERSION     DAECNO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.045"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..218
                     /locus_tag="KKO08_002054"
     CDS             <1..218
                     /locus_tag="KKO08_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6318296.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KKO08_002055"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="KKO08_002055"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBD6318297.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KKO08_002056"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="KKO08_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBD6318298.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="KKO08_002057"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="KKO08_002057"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBD6318299.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="KKO08_002058"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="KKO08_002058"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBD6318300.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="KKO08_002059"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="KKO08_002059"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBD6318301.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="KKO08_002060"
     CDS             7229..8179
                     /locus_tag="KKO08_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBD6318302.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="KKO08_002061"
     CDS             complement(8220..8690)
                     /locus_tag="KKO08_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBD6318303.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="KKO08_002062"
     CDS             complement(8745..9947)
                     /locus_tag="KKO08_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBD6318304.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="KKO08_002063"
     CDS             complement(10034..10576)
                     /locus_tag="KKO08_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318305.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="KKO08_002064"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="KKO08_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBD6318306.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="KKO08_002065"
     CDS             complement(11319..11921)
                     /locus_tag="KKO08_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBD6318307.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="KKO08_002066"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="KKO08_002066"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBD6318308.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="KKO08_002067"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="KKO08_002067"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBD6318309.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="KKO08_002068"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="KKO08_002068"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBD6318310.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="KKO08_002069"
     CDS             16319..17209
                     /locus_tag="KKO08_002069"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBD6318311.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="KKO08_002070"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="KKO08_002070"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBD6318312.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKTIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="KKO08_002071"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="KKO08_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBD6318313.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="KKO08_002072"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="KKO08_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBD6318314.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KKO08_002073"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="KKO08_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBD6318315.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KKO08_002074"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="KKO08_002074"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBD6318316.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KKO08_002075"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="KKO08_002075"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBD6318317.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KKO08_002076"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="KKO08_002076"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBD6318318.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KKO08_002077"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="KKO08_002077"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBD6318319.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KKO08_002078"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="KKO08_002078"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBD6318320.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KKO08_002079"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="KKO08_002079"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBD6318321.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KKO08_002080"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="KKO08_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBD6318322.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="KKO08_002081"
     CDS             complement(27384..27845)
                     /locus_tag="KKO08_002081"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD6318323.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="KKO08_002082"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="KKO08_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBD6318324.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="KKO08_002083"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="KKO08_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         7087 a         7856 c         7300 g         6993 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa aaccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DAECNO010000046        29048 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.046, whole genome shotgun sequence.
ACCESSION   DAECNO010000046 DAECNO010000000
VERSION     DAECNO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.046"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            108..1139
                     /gene="mdtN"
                     /locus_tag="KKO08_002084"
     CDS             108..1139
                     /gene="mdtN"
                     /locus_tag="KKO08_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBD6318325.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1139..3187
                     /gene="mdtO"
                     /locus_tag="KKO08_002085"
     CDS             1139..3187
                     /gene="mdtO"
                     /locus_tag="KKO08_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBD6318326.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3184..4650
                     /gene="mdtP"
                     /locus_tag="KKO08_002086"
     CDS             3184..4650
                     /gene="mdtP"
                     /locus_tag="KKO08_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBD6318327.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4848..5087
                     /locus_tag="KKO08_002087"
     CDS             4848..5087
                     /locus_tag="KKO08_002087"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318328.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5097..5267
                     /locus_tag="KKO08_002088"
     CDS             5097..5267
                     /locus_tag="KKO08_002088"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD6318329.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5316..6728
                     /gene="fdhF"
                     /locus_tag="KKO08_002089"
     CDS             5316..6728
                     /gene="fdhF"
                     /locus_tag="KKO08_002089"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBD6318330.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6729..6995
                     /locus_tag="KKO08_002090"
     CDS             6729..6995
                     /locus_tag="KKO08_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HBD6318331.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7089..7778
                     /locus_tag="KKO08_002091"
     CDS             7089..7778
                     /locus_tag="KKO08_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBD6318332.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="KKO08_002092"
     CDS             complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="KKO08_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBD6318333.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="KKO08_002093"
     CDS             complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="KKO08_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBD6318334.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="KKO08_002094"
     CDS             complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="KKO08_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBD6318335.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10480..12198)
                     /locus_tag="KKO08_002095"
     CDS             complement(10480..12198)
                     /locus_tag="KKO08_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBD6318336.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="KKO08_002096"
     CDS             complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="KKO08_002096"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBD6318337.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="KKO08_002097"
     CDS             complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="KKO08_002097"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBD6318338.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="KKO08_002098"
     CDS             complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="KKO08_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBD6318339.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="KKO08_002099"
     CDS             complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="KKO08_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBD6318340.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16279..18237
                     /gene="acs"
                     /locus_tag="KKO08_002100"
     CDS             16279..18237
                     /gene="acs"
                     /locus_tag="KKO08_002100"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBD6318341.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18437..18751
                     /locus_tag="KKO08_002101"
     CDS             18437..18751
                     /locus_tag="KKO08_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBD6318342.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18748..20397
                     /gene="actP"
                     /locus_tag="KKO08_002102"
     CDS             18748..20397
                     /gene="actP"
                     /locus_tag="KKO08_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBD6318343.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20575..21867
                     /locus_tag="KKO08_002103"
     CDS             20575..21867
                     /locus_tag="KKO08_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD6318344.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22021..23670)
                     /locus_tag="KKO08_002104"
     CDS             complement(22021..23670)
                     /locus_tag="KKO08_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBD6318345.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="KKO08_002105"
                     /gene_synonym="yjcD"
     CDS             complement(23821..25170)
                     /gene="ghxP"
                     /locus_tag="KKO08_002105"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBD6318346.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="KKO08_002106"
     CDS             complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="KKO08_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBD6318347.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26426..26749
                     /gene="soxS"
                     /locus_tag="KKO08_002107"
     CDS             26426..26749
                     /gene="soxS"
                     /locus_tag="KKO08_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBD6318348.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="KKO08_002108"
                     /pseudo
     CDS             complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="KKO08_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28758..29039
                     /locus_tag="KKO08_002109"
     CDS             28758..29039
                     /locus_tag="KKO08_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBD6318349.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7288 a         7777 c         7349 g         6634 t
ORIGIN      
        1 gggcaaatat caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg
       61 ccatgctgtt ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc
      121 cgaaaaaagc acctcgcagt aaattccctg ctctgttagt ggttgcgttg gcgctggttg
      181 cccttgtttt cgttatctgg ctcgtagaca gtgcgccatc aactaatgac gcttacgcgt
      241 cagcagatac cattgatgtg gtgccggaag tcagcggtcg cattgtagaa ctggcggtca
      301 ccgacaacca ggcggtgaaa cagggcgatt tgctgttccg catcgacccg cgcccgtacg
      361 aagccaacct ggcgaaagcc gaagcctccc tcgcggcgct ggataagcaa attatgctca
      421 cccagcgtag cgttgacgcc caacagtttg gtgccgactc ggttaatgcc acggtagaaa
      481 aagcccgtgc cgccgcgaaa caggccacag atacattacg ccgcaccgag ccattactga
      541 aagagggttt tgtctcagca gaagacgttg accgtgcaag aacggcgcag cgcgccgcag
      601 aagcggatct taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg
      661 tcgatgcgct ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactac
      721 atctggaaat gacgaccgtt cgcgcgccat ttgatggccg gatcatttcc ctcaaaacct
      781 ccgtcgggca atttgcttct gccatgcgcc ctatttttac cctcatcgac actcgtcact
      841 ggtacgtgat cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg
      901 caacgattcg cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg
      961 gctacggcgt gctaccggat gacggcggcc tggtgctggg cggcctgcca aaagtgtctc
     1021 gttctattaa ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac
     1081 ctgacccgga aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat
     1141 gagcgcgctc aactccctgc cattaccggt ggtcaggctg ctggcgttct ttcatgaaga
     1201 gttaagcgag cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct
     1261 gctggtgatt ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt
     1321 gctgttttac ggtatcggta ttcagtcgaa cgcgttttac accaaatttg tggcgatctt
     1381 gtttgtggtt gccacggtgc tggagatcgg cagcctgttt ttgatctaca aatggtcata
     1441 cggcgaaccg ttgatccgat tgatcatcgc cgggccgatc ctgatgggct gcatgttttt
     1501 gatgcgcacc catcggctgg gactggtctt tttcgctgtc gccatttacg ggcaaacctt
     1561 ccccgccatg ctcgactatc cggaagtagt cgtgcgctta acgctgtggt gtatcgttgt
     1621 tggcctctat ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc ccaatcgtgc
     1681 cattacgcaa atgcatcaag cgcttaatga tcggcttgat gatgccatta gccacctgac
     1741 ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa
     1801 actgaatgtc ttttgtctcg cggacgatgc caactggcga actcaaagcg catggtggca
     1861 aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc
     1921 ttttgctgat tctcaggcaa ttattgaatt tcggcaaaaa ttagcttcag aaatcaacaa
     1981 gctgcagcat gccgttgctg aaggtcagtg ctggcaaagc gactggcgga tcagtgaaag
     2041 tgaagcggtg gcggcacggg aatgtaacct ggagaatatc tgccagacgt tgttacaact
     2101 gggccagatg aacccgaata cgccgccaac gcccgccgcc aaaccgccat caatggtcgc
     2161 cgatgctttt accaatccag actatatacg ctacgcggta aaaacattgc tcgcctgttt
     2221 gatctgttac accttttaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac
     2281 ctgcgtgatc gtcgctaatc caaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt
     2341 tggcggggcc ttttgcggcg cgattctggc gctgttattc acgctactgg tcatgccctg
     2401 gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt tggccgcatg
     2461 gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc
     2521 gctcgccacg ctcgaaaacg tttttggccc agtgtacgac ctggtggaaa ttcgcgatcg
     2581 cgccctgggt atcatcattg gtaccgtggt gtccgcgatg atttacacct ttgtctggcc
     2641 tgaaagtgaa gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttaagtaa
     2701 agtaatgcgg atcccacgcc agcaggaagt cacggctctg cgcacttatc tgcaaattcg
     2761 tatcggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggcgctgga
     2821 gcgtcaactg gacagcgaag aacgcgcctt actgattgaa cgttcgcaaa cggttattcg
     2881 tcagggccgc gatatccttc acgcctggga tgccacctgg aattcggcgc aggcgctgga
     2941 taacgcacta cagccggaca gagcaggtca gtttgccgac gccctggaga aatacgctgc
     3001 cggtctggca accgcactca gccgttctcc tcaaataacg cttgaagaga cacccacctc
     3061 gcaggccatc ctgcccacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg
     3121 cttgccagac tggacagccc cggcattaac gcccgccacg gaacaggcac aaggagccac
     3181 gcaatgatca atcgtcaact ttcacgtctg ctgttgtgca gcattctcgg cagcacgacg
     3241 ctgatttccg gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg
     3301 gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag
     3361 tggtggaaac aactcaatga cccgcagttg gatgccctga tccaacggac gctaagtggt
     3421 tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcaatcgca ggccgatttg
     3481 ttagatgccg gttcacagtt gcaggtggca gcgttaggga tgcttaaccg ccaacgtgtc
     3541 tcggcgaacg gctttttaag cccttatgcg atggatgcgc ccgcactggg gatggacggg
     3601 ccgtactata cggaagccac agtaggtttg tttgccggac tggatcttga tttgtggggt
     3661 gtgcatcgct cagcggttgc cgccgccatt ggcgcgcata atgccgcgct ggcagaaacc
     3721 gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc
     3781 agctatcaga tgctcgatct gttagaacaa actcgcgatg tgattgatta cgcggtgaaa
     3841 gcgcaccaaa gtaaagtggc gcacggtctg gaagcacaag tgcctttcca cggcgcgcgg
     3901 gcacagattc tggcggtcga taaacaaatt gctgccgtca aagggcaaat caccgaaacg
     3961 agagaatctc tgcgcgcatt gattggcgcg ggagccagcg atatgccgga gatcaaacct
     4021 gtggcattac cacgagtcca gaccggcatt ccggcgacac tctcttatga gttgctcgcc
     4081 agacgcccgg atctgcaagc catgcgctgg tatgttcagg cgtcattaga tcaggtggat
     4141 tccgcgcggg cgttgttcta tccgagcttt gatatcaaag cgtttttcgg ccttgatgcc
     4201 atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaacttcat cccgggtctg
     4261 aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac gcgcgccgcc
     4321 agcaacatga tgattgaacg ttacaaccag tcagtactga acgcggtgcg tgacgttgcc
     4381 gtcaacggca cgcgtctgca aacgctcaac gacgagcgag aaatgcaggc tgaacacgtg
     4441 gaagcaacgc gcttcaccca gcgcgctgcc gaggccgcct atcagcgcgg cttaaccagc
     4501 cgcttacagg ccactgaagc ccggttgcca gtacttgccg aagagatgtc attactgatg
     4561 ctggacagcc gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat
     4621 caggcggctc ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc tgtcacgcgg
     4681 tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa
     4741 atagtgcccg taatatcagg gaatgacccc aaataaaatg tggcataaaa gatgcatact
     4801 gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg
     4861 tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca
     4921 aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg
     4981 gttattatgg ctgggatttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc
     5041 ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgagat gaggcactga
     5101 attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga
     5161 cgaccggctc ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc
     5221 gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg
     5281 ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg
     5341 ataataccga tttagtgttc gttttcgggt acaacccggc ggattcccac ccaatcgtgg
     5401 cgaatcacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca
     5461 aaattgaaac cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg
     5521 cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa tctgtacgac aaagcgttcg
     5581 tcgcttcccg tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg
     5641 agtcggttga agatatcacc ggtgtcagcg ccagtgagat tcgtcaggcg gcacggatgt
     5701 atgcccaggc gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag ttctaccagg
     5761 gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gaccagtaac ctcggtaagc
     5821 cgcatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg
     5881 gcgcgctgcc ggatacgtat ccgggatacc agtacgtgaa agatccggct aaccgcgaaa
     5941 aattcgccaa agcctggggc gtggaaagcc tgcctgctca taccggctat cgcatcagcg
     6001 agctgccgca ccgcgcagcg catggcgaag tgcgtgccgc gtacattatg ggcgaagatc
     6061 cgctgcaaac tgatgcggag ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg
     6121 tcatcgttca ggacatcttt ataaccaaaa ccgcgtcggc ggcggatgtc attttgccgt
     6181 caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt
     6241 tcttcaaagc agttgaaccg aaatgggatc tgaaaacgga ctggcaaatc atcagtgaaa
     6301 tcgccacccg tatgggttat ccgatgcact acaacaacac ccaggagatc tgggatgagt
     6361 tgcgtcatct gtgcccggat ttctacggtg cgacttacga gaaaatgggc gaactgggct
     6421 tcattcagtg gccttgccgc gatacttcag atgccgatca ggggacttct tatctgttta
     6481 aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc
     6541 caatcgacaa actcaccgac gagtacccga tggtactgtc aacggtgcgt gaagttggtc
     6601 actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac
     6661 ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat
     6721 tggtttgagt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc
     6781 cgaacaaagg ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg
     6841 ttaccgaaag cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg
     6901 tcgagccgat ctccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt
     6961 tgaaaactcg cctgcgcgaa gcggcactgg cgtaatacag tcctttctac agcctccttt
     7021 cggaggctgt ttttttatcc attcgaactc tttatactgg ttactcccta cccaatcgta
     7081 ttatcaaaat gaaaaaaatt atcgcattga tgttgttttt gacattcttt gcccacgcga
     7141 acgactccga gcctggcagc cagtatttaa aggcagcaga ggccggggac cgacgtgcac
     7201 aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg
     7261 cacagaaagc cgccgacagc ggtgatgctg atgcctgcgc gctgttggcg cagatcaaaa
     7321 tcaccaatcc ggtcagtctg gactatccac aagcaaaagt tcttgcagag aaagcggcgc
     7381 aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat actcaagcgg
     7441 gtaaaccgga ttatccaaag gcgatttcgc tgttagaaaa cgcctcggaa gatctggaga
     7501 atgactccgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca
     7561 ttaaggccga cgatgacaag gcaacctggt atttcaaacg cagctctgca atttcccgaa
     7621 ccggttattc cgagtactgg gcgggaatga tgttcttaaa cggtgaggaa ggttttatcg
     7681 agaagaacaa acaaaaggcg ctgcactggt tgaacctgag ctgtatggaa gggtttgata
     7741 ccgggtgtga agagtttgaa aaattaacca acggttaagg taggcctgat aagacgcggc
     7801 aagcgtcgca tcaggcatcc acacattgcc gggggatatc cccggcaatc ttcaattatt
     7861 gatccgcagt tttatcaaat ttgcccagca cttcacgctc ataagccagc gcctttttgc
     7921 ggtcaaattt gtgttcccac ttggcaatca ccagcaccgc cagcgcatta cccaccacgt
     7981 tcagcgcagt acgcgccatg tcgaggatac ggtcaacacc agcaataaac gccagacctt
     8041 ccagcggaat acctacgcta cccagcgttg ccagcaacac cacaaacgac acgccagcca
     8101 cgccagcaat ccctttcgag gtcaccatca gcgtcagcac cagaatgatt tcctgccaga
     8161 tggacaggtc aatgccatac agctgagcga tgaaaatagc ggcaatactt tgatacagcg
     8221 tcgaaccatc aaggttaaaa gagtaaccgg tcggcaccac gaagctggtg atcgacgccg
     8281 gtgctccgta ggcttccatc ttctcaataa ttcgcggcag tacgctttca gagctggcag
     8341 tggagtacgc cagaatcagc tcatctttca gaatacgaat caggatccag acgcttaacc
     8401 cgcacagacg tgccacaatt cccagcacta ccagcgcgaa gaacagaatg gcgaaatgca
     8461 ccagcagcac cagtttcgcc agtggccaca gagacgagaa accaaagtta gccaccgtca
     8521 ccgcaatcag cgcaaacaca cccaccggtg cataacgcat caccatgtga gtcactttaa
     8581 acatggtttc agagatggag cggaacacgg tcaccagcgg ttcacgatgc gtcgcgggca
     8641 gggaagaaag ccccagacca aacagcaccg agaaaaagat gatcggcagc atttcgcctt
     8701 tcgccatcga cgccacaatg ttcgtcggca ccagcgacaa aatcgtgccc ataatgccgt
     8761 gggaactgct ttgtaccgct tccgtagtgc tctgatattt cgagatatcg acggtcgcca
     8821 gctgcgacat atccaccccg gcaccgggct ggaagacgtt cgccagagtg atccccaaaa
     8881 tgatggcgac ggtggtgatc acctcgaagt aaataatggt tttcgcgcca atacgcccga
     8941 gctgtttggc atcaccaaca cccgcgatac ccaccaccag cgtggagatc acaatcggca
     9001 caacaatcat tttgatcaga tggatgaaga tatcacccgc cggagagagc aaattgacga
     9061 ccagccagtc gcggctgtcg ctatggtagt gcaggtagct tcccaggaga atgcccagca
     9121 ccatagcaaa cagaatctgc caggccaggc tgaattttat atttttcatc agaataactt
     9181 cctcaatgga gcgccgttcc ccgttcagaa acgcgagaac gtgacatact gccagggcat
     9241 gaattgtgtt gcgttggttt gtagattttt ttaacgctta tgatcagtat catttgcgcg
     9301 gtatgttgcg caacccttta acaacagagc ttttcgctgc attaatgcat aataacgcga
     9361 gtttatggca ggattaatga ataaccattt attatggaaa gatttttatt tgtttaatct
     9421 gcaataatac tgattggtga ttatttttgg catatgttca ttcgattatt ttttatgcag
     9481 ctcaatgaca taatcttcat caccagatga ttaatccgat ctccgctgca acagttttgc
     9541 catattaatc gactcgacca gctgtgtccg gttgatccgg ggtgagttga gatccatcac
     9601 tttttgccat agttcgatgg cttgcgcgta attcgcctgc ataaacgcat cggaagccag
     9661 cagcatcaga gcggtgattt cgttagagtc cagcgcgagg gctttgtcga tcattgcgcg
     9721 agtctgggcg gtcatatgct ggctggcctg gtaataaagc accgtcgcca gcgccgcata
     9781 cagttcagcg ttctcaccac gtagttgcaa cgcctgacga tacgccagca gcgaattgct
     9841 gtaatcgttt tgccacagat agtactcgcc cagtaacgcc cattgttcgc tgttttgtgg
     9901 attagcgcgg attttatcct gcaatgcctg aagctgcgct tccggggttt gctggctggc
     9961 aaactgatgt agcggatcgc gctgacgctg atactccgca cgtaccgcct gccattttgg
    10021 gcttaacaga taactgccga cacagaggaa taccatcaat atcgtgagtg ttgtcagcat
    10081 tttcaccggt atttgaggct gtttcatcgc ttagccctca ctcgccagag gatcagtgcc
    10141 atcagcaata acaacaccac tggcagcgcc cacaacacca gcgtctgacc cgttaacggt
    10201 gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc agccaatgat ttcgacttcg
    10261 ttcttcccct ccgacaccat gctataaacc tgatggcgca tactgaccgc caccggcgca
    10321 ttggattcga gtaagttttg attctggcac tgcggacaac gtaactggct ggcaatattt
    10381 aacgcctgtt gctgttgttg cggattggcg aattgccagg tgtctacaac ctgagcgcgc
    10441 gcaaaacagg taaataacag cagtagcgtg agaagccctt tattcatcgc gcttcttccc
    10501 ccgccatccg cttaacaatg cgcccgcaat catcaacaat cctccccccc agatccagcg
    10561 cacaccgctt tgtacataca aacgaaaagc gtaacgatcc gctctggttt tttcacccat
    10621 gaccgcatac cagtcatgga tgccgttcca gcgaattgac ggttccatca tttgctgacg
    10681 gcgtgcttca taaaaacgcc gctccggcgt taattcacca atgcgttgct gatggtcaaa
    10741 cagcgccact atcgcttttt cgctggtgta attgcctctg gcttgcagat cgagtcgctc
    10801 aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat
    10861 ctcctgacga ctgacactgg aaaccacgat ccccgcggca aataacagca cgccagcatg
    10921 agctaccagc gccggaagct gcacgcgttt gccagagaca aacgtcgcca gcacaatcac
    10981 caccagcatc aacagaccaa acggtaacgt cgcgcggtta aaatacggcg cgccaacgga
    11041 gaggcgtccc cagcccagca ggccgtaaat catcggataa agcgttccca ccagcacgat
    11101 cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt aacatttccc gcgataaccc
    11161 cgaaaaacgc accgccgggc caccgtcccg cgctcgccag ccatacagag ccaaagacgc
    11221 aaggctaatc agtgcaaaca ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa
    11281 cgcatgaacc gaaaccagaa tgccagaacg gacaattaag gtgcccagca gcgacagcat
    11341 cagagtaact atcgccagca acagcgacca gtggcggaaa atcccccgct ggcgtgtcag
    11401 ggacaaactg tgcagcagcg cagtggcaga aagccagggt aataaagagg cgttttccac
    11461 cggatcccag aaccaccagc cgccccagcc gagttcgcag taggcccacc aggaaccgag
    11521 gatgatcccc gccgttaatg cactccagcc aggtaacgcc cagcgccagc aaattcgggc
    11581 gctggccgca tcaaactcgc cgcgcagtag actcgccagc gccacgctcg ccgctaccat
    11641 caaaccgcca tagccaaggc aaagcagcgg tggatgaaag ataagaccgg gatgttgcag
    11701 catcggattg agatcgcggc cttcgattgc tggtggaaat atccgcacga agggatcgga
    11761 ccacagcacc acaaacagta gcagtgcggc gagcatgaga gataaaacgg ctagcgtcag
    11821 cggaaatagc ggatcgcttt gctgccgata atgccaggca aacagcgcgc tccaggcgga
    11881 aagcagcagc acccaaagca gcagcgaacc ttcatgaccg ccccacaccg ccgccagttt
    11941 gagttcccac gacagcaggc tgtagctatg ttgggcgaca taaatgaccg agaaatcgct
    12001 gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc aggagcgcga actgcgccag
    12061 aatgccgatg caagtgagtc gcatcatggc aggccagcgc aaccgcactc ccgcaaaggc
    12121 cgtcaacggg gtcaacacgt tgaccccgag acttaacaac aacgccagaa aaccgacttc
    12181 aggaaggaat acgtccagaa accacctctt ttctggctta cgccaccgtc aactgtccgg
    12241 cgtagagaat gaaaaagcgc agcatcaaca cgccgaccag actggcccca cacgccgcca
    12301 gcacggcagg aatgccggaa ctgcgattga cccacggttt gagcaacatt ggcacaatca
    12361 gccccagccc ggcgacacca agccagaacc accacgtcca gaaaccgcca cctaatgccg
    12421 ccaccagcgc acgaacttta ccgtcatcgc ccagcgccag accgacaaaa aacgccacca
    12481 gcaggaagat ttcaccccat accacgggga tttccatccg gtgaacaaag tgcgcttccg
    12541 tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg
    12601 acgagatgcc ggagaagagg agcagcaccg gcaggctcgg gttattgagg aacggatacg
    12661 atttcagcgc cgacagcaga aagccggtat aagcccccag cagcaccgcc aacaccagca
    12721 tcagcgtttc cagtccgcga tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc
    12781 ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc atgttcaaag attttcgcca
    12841 gccacagcac cagcaccacc atgtagagct gaaacagcat cacccccatc gacatcaccg
    12901 aggtaaaact gtagtggaac atcagcttcc agaaggtcca cggtcttgtc aggtggaaga
    12961 cgaggatcaa cagaccgagg atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca
    13021 acgtactgtc tgcaccgccc gcctgcgggt agaagcgacg taacagcacg gccagcgtca
    13081 ccagaccggc agaaatacca atcaaaaaca ggtagatggc aatcggccag tcccacacca
    13141 gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc acctccccgt atttaaacgg
    13201 tacgcggtac agcttcggtt tggttcccag cgccagcttg tagcggtaag taggcttctg
    13261 gcgcagcagt tgcgaaatct cactgttagg atcgtccaga ttgccaaacg tcagcgcctt
    13321 cgtcgggcaa gcttcaacgc acgcgggaag cttaccggcc tgcaaattgg tcttacgaca
    13381 gaaatcgcat ttgtccgccg tcttcgtgac cggatggata aagcgcacgc ggtacggaca
    13441 ggcggcgatg cagtactgac aaccgacgca gagatcagga ttaacatcga caatcccgct
    13501 agcggcatca cgaaaagacg caccggtcgg gcagacgtca acgcacggca catgatcgca
    13561 gtgctggcaa gacttacgga agaagcgata tttcacatca ggaaattcgc cctgcggctc
    13621 gctacgaata atcgtcaggc gcgagacgcc ttccggcacc ttgttcactt cccgacaagc
    13681 atccatacag gcggtacagc cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac
    13741 cccattgata ttcaacgttt tcgccaccac gcgccctgcg gtgccgctga cggctgccag
    13801 cacgccgacg ccggtgagaa actggcgacg agaccaggtc atggctgctc cttaagcaac
    13861 ggaacagacg ccgggttaaa gttcggattg gtgcgctgat cgctgtggca atcgacgcaa
    13921 atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa
    13981 tggcagctgg cgcacgccac tttggttacg tggacatcgt gcggccagaa cgctttttgc
    14041 aactgttccg gcagatgaca ggacatacag acgctgttct gctctccgac cttatacatc
    14101 ggctcgttaa agcgcatcac atctttcacc ccttcgcggt gttgcggtga tgcttggccg
    14161 tgacagttgg tgcaggtgac cggcaatttg ttattcgggt tgataacgga agcatgtttg
    14221 ccatgcatac cttcggtatc tggtttatga cagtccagac aggcggcatc cgggttacgc
    14281 tgctgggtaa cttcgtagcg atcaccagat gcctgcaccg caggggtagc ggtaattccg
    14341 ttcaggctcc acaacaggcc cgacgccagc accccagcag ttaataacga acgtaatacg
    14401 ctcatattca ctccattgtg ttttccgccc cttgcgaggc ggaacggggt tattggctta
    14461 acagaccgtt tttacgtgcc tgctcttccc actgcgggat caccgttttg atgaagtcct
    14521 gcttctcggc cttgatttgt tccatgttca ggccaatggc ctgctgggct ttctctttgg
    14581 tcgagatatc cgggatctgg atttcatggg tgataccttt ggtcgccagc aggcgcgcca
    14641 gtttggtgcg cgcatccgcc gctttatcca tcgccgtacc gagcatccgt agaccttctt
    14701 ccggtgcgtg catatgaata ccgtgggaag cgatcgccag atcccaacgc cactgggcat
    14761 gacggatatc gtcctgaatc ggcttcattt cggcttccgt cgcgcctgca tccagcgccg
    14821 ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac cttgattttc aggtcgttaa
    14881 tcgattgctt acgttccgcg accacttttt gcaaggcagc tttatcctgg gtatggcagt
    14941 tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat tttatggtcg gtgtagagtt
    15001 tgccttcggc gttctgcact tttggcatat ggcagtcgat acaggtcacg ttgtttttac
    15061 cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg cgctttcagc attggcgttt
    15121 tcgacaggga gttagtccag tcagagaagg caattttgtc gtaatactgc tccatatttt
    15181 cgactttcat gccgtcatcc cacgggaatt taaccgcttt gtttttgccg tcgaagtaat
    15241 actccacatg gcactgaccg caaaccatcg attgctggtc gaaacgtccg gctttctcaa
    15301 aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg acgggaaagg gttaactccg
    15361 gtttgccttt ggcgaactct ggtgaggcgg tgttatggca gtcggcacaa cctaagttgt
    15421 tgacgatttc cgggccgccg cgcgcccatt taccgtggaa gtagccatct tcgccgtctt
    15481 tctggatcag acgcgccaca tccgggcttt tacaactcca gcacgccatc ggtagcggac
    15541 catcttcagc gtttttcggc gcaccggtac gcagggtttc acgcacatcg gtcacagcaa
    15601 aggcatgtcc acgaggcttg ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca
    15661 ccagccgtgg atcttccgcc agggcgtcaa cacgctctga ctgttccgag gtggctttcc
    15721 aggagagata ttgatcgtga tgctgcgggg caaaggtttc attcttcgct tccacagtta
    15781 caggttttgc gggagctgcc gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa
    15841 tcaataagct gaagatacgg cgtgcgttta tttttatcct tgtcataggg gcttcatccg
    15901 aattgcgcca ttgttgcaat ggcggttttt attgtttttc acgacagtaa ccgcacctac
    15961 actgtcatga cattgctcac ccctatgtgt aacaaataac cacactgtga atgttgtctt
    16021 taatcaattg taagtgcatg tgaaatacca ctttagagtt agtcagtatc ttcctctttt
    16081 tcaacagcat gcataactgc atgttcctca aagaattaat caacttttgt tggtgacctt
    16141 caaaaattac cctgccgttt atttgcacca ttctactttt gcgtgatctg tcgcccaaat
    16201 actaaacaaa actgccaata cccctacatt taacgcttat gccacatatt attaacatcc
    16261 tacaaggaga acaaaagcat gagccaaatt cacaaacaca ccattcctgc caacatcgca
    16321 gaccgttgcc tgataaaccc tcagcagtac gaggcgatgt atcaacaatc tattaacgta
    16381 cctgatacct tctggggcga acagggaaaa attctcgact ggatcaaacc gtaccagaag
    16441 gtgaaaaata cctcctttgc ccccggtaat gtgtccatta aatggtacga ggacggcacg
    16501 ctgaatctgg cggcaaactg ccttgaccgc catctgcaag aaaacggcga tcgcaccgcc
    16561 atcatctggg aaggcgacga cgccagccag agcaaacata tcagctataa agagctgcac
    16621 cgcgacgtct gccgcttcgc caataccctg ctcgagctgg gcattaaaaa aggtgatgtg
    16681 gtggcgattt atatgccgat ggtgccggaa gccgcggttg cgatgctggc ctgcgcccgc
    16741 attggcgcga tgcattcggt gattttcggc ggcttctcgc cggaagccgt tgccgggcgc
    16801 attattgatt ccaactcacg actggtgatc acttccgacg aaggcgtgcg cgccgggcgt
    16861 agtattccgc tgaagaaaaa cgttgatgac gcactaaaaa acccgaacgt caccagcgta
    16921 gagcatgtgg tggtactgaa gcgtactggc ggaaacatcg actggcagga agggcgcgac
    16981 ctgtggtggc acgacctggt tgagcaagcc agcgatcagc accaggcgga agagatgaac
    17041 gccgaagatc cgctgtttat tctctatacc tccggttcta ccggaaaacc aaaaggcgta
    17101 ctgcacacta ccggcggtta tctggtgtac gcggcgctga cctttaaata tgtctttgat
    17161 tatcatccgg gtgatatcta ctggtgcacc gccgatgtgg gctgggttac cggacacagt
    17221 tatttgctgt acggcccgct ggcctgcggc gcgaccacgc tgatgtttga aggcgtaccg
    17281 aactggccga cgcctgcccg tatggcgcag gtggtggaca agcatcaggt caatattctc
    17341 tataccgcac ccacggcgat ccgcgcgctg atggcggaag gcgataaagc gattgaaggc
    17401 accgaccgtt cgtcgctacg cattctcggt tccgtgggcg agccaattaa cccggaagcg
    17461 tgggagtggt actggaaaaa aatcggcaac gagaaatgtc cggtggtcga tacctggtgg
    17521 cagaccgaaa ccggcggttt catgatcacc ccgctgcctg gcgctaccga gctgaaagcc
    17581 ggttcggcaa cacgtccgtt cttcggcgtg caaccggcgc tggtcgataa cgaaggtaac
    17641 ccgctggaag gggctaccga aggtagcctg gtgatcaccg actcctggcc tggtcaggcg
    17701 cgtacgctgt ttggcgatca cgaacgtttt gagcagacct atttttccac cttcaaaaat
    17761 atgtatttca gcggcgacgg cgcgcgtcgc gatgaagatg gctattactg gatcaccggg
    17821 cgtgtggacg acgtgctgaa cgtctccggt caccgtctgg ggacggcaga gatagagtcg
    17881 gcgctggtgg cgcatccgaa gattgccgaa gccgccgtag taggtattcc gcacaatatt
    17941 aaaggtcagg cgatctacgc ctatgtcacg cttaatcacg gggaggaacc gtcaccagaa
    18001 ctgtacgcag aagtccgcaa ctgggtgcgt aaagagattg gcccgctggc gacgccagac
    18061 gtgctgcact ggaccgactc cctgcctaaa acccgctccg gcaaaattat gcgccgtatt
    18121 ctgcgcaaaa ttgcggcggg cgataccagc aacctgggcg atacctcgac gcttgccgat
    18181 cctggcgtgg tcgagaagct gcttgaagag aagcaggcta tcgcgatgcc atcgtaaccc
    18241 acaattgccg gatgcgaatc ggtaacggtt tgtaggcctg ataagacgcg acagcgtcgc
    18301 atcaggcatt gattgccgga tgcggcgtaa acgccttatc cggcctacat tcggcaaggg
    18361 ttacccgagc gttaaccttc tcccataagg gagcgggaat taaaacaatc cctacattac
    18421 ctctggagaa tctgtgatga atggtacgat ttatcagcgg atagaagaca atgcgcattt
    18481 cagggagtta gtcgaaaaac ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc
    18541 agtttatatc ggctttattt tactgatcgc cttcgcgccc ggctggctgg gcacgccgct
    18601 gaatccgaac accagcgtca cacgcggtat tccaattggt gttggagtga ttgtgatctc
    18661 ctttgttctc accggtatct acatctggcg ggcgaacggc gaattcgacc gtcttaataa
    18721 tgaagtcctg catgaggtac aagcatcatg aaaagagttc tgacggcgct tgccgccaca
    18781 ctccctttcg cagctaacgc cgcggatgct attagcgggg ccgtagagcg ccagccaacg
    18841 aactggcagg cgattattat gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac
    18901 tgggcatcaa aacgcgtacg ttctcgtagc gactactaca ccgcaggcgg caatatcact
    18961 ggcttccaga acgggctggc gattgccggg gactatatgt ccgccgcctc attcttgggg
    19021 atctccgcgc tggtgtttac ctccggctat gacgggctga tctactcgtt gggcttcctg
    19081 gtgggctggc caatcattct gtttctgatt gccgaacgtc tgcgtaacct gggacgctac
    19141 acctctgccg atgtggcctc ttatcgtctg aaacaagggc cgattcgtat tctttcggcc
    19201 tgtggttctc tggtagtggt ggcgctttac cttatcgctc agatggtagg cgcaggtaaa
    19261 ctgatcgagc tgctgtttgg ccttaactat cacattgcgg tggtgctggt cggcgtgctg
    19321 atgatgatgt acgtcctgtt cggcggcatg ctggcgacca cctgggtaca aattatcaaa
    19381 gctgtgctgt tgctgttcgg tgccagcttt atggccttta tggtgatgaa acacgtcggc
    19441 tttagcttca acaatctgtt cagcgaagcg atggcggtac acccgaaagg tgtcgacatc
    19501 atgaaaccgg gcggactggt gaaagatccg atctccgcgc tctctctggg gctgggactg
    19561 atgtttggta cggcgggctt gccgcacatt ctgatgcgct tctttacagt cagcgatgcc
    19621 cgcgaagcac gtaagagcgt gttctacgcc accggattta tgggctactt ctatattctg
    19681 acctttatta tcggcttcgg cgcgatcatg cttgttggtg cgaatccgga atataaagac
    19741 gcggcgggcc atctgattgg tggtaacaac atggcggccg ttcacctggc gaatgcagtg
    19801 ggcggcaacc tattcctcgg ttttatttca gcggttgctt tcgccactat cctcgcggtg
    19861 gttgcggatc tgacactggc gggcgcatcg gcggtttcgc atgacttgta cgctaacgtc
    19921 ttcaaaaaag gcgcgaccga acgtgaagag ctgcgggtat caaaaatcac cgtactgatc
    19981 ctcggcgtga ttgcgattat cctcggcgtg ctgtttgaga atcagaacat cgcctttatg
    20041 gtaggccttg cgtttgccat cgcggcgagc tgtaacttcc cgatcattct gctttccatg
    20101 tactggtcga aactgactac ccgtggcgcg atgctgggcg gctggctggg gctgattacc
    20161 gcagtggtgc tgatgatcct cggcccgact atttgggtac agatcctcgg tcacgaaaaa
    20221 gccatcttcc cgtatgaata cccggcgctg ttctctatca gcgtggcatt cctcggcatc
    20281 tggttcttct cggcaaccga taactcagcg gaaggggcgc gcgagcgtga actgttccgc
    20341 gcgcagttta tccgctccca gaccggcttt ggcgttgagc aaggccgcgc acattaatct
    20401 ctccccttcc ccggtcgcct gaccggggaa tacgcttcct ctccaacatg catcaccttt
    20461 tcccataata ttaaacaaat aaactcatta aaaaatgagc gatttttgac agtcgtagaa
    20521 aatgataatg cagagaatat gccttttctt tcttgttaat tataaggata ttttatgcgc
    20581 tataatggtt taaataatat gtttttccct ctttgccaga ttaacgataa ccactctatc
    20641 acaagttcat cacatacaaa gaaaacaaaa tcagataatt acagcaaaca tcataaaaac
    20701 acgttaattg acaataaagc cctctctctt ttcaaaatag atgatcatga aaaagtgata
    20761 ggcttgattc agaaaatgaa aagaatttat gatagtttac catcaggaaa aatcacgaaa
    20821 gaaacggaca ggaaaataca taaatatttt atagatatag cgttatatgc aaataataaa
    20881 tgtgacgata gaattacgag aagagtttac cttagtaaag aaaaggatgt atccattaag
    20941 gtggtatatt ttataaataa tgtcgccgtc cataataata ctatcgaaat tccacagata
    21001 gtaaatggtg gttacgattt ttcacacctt agcctgaagg gtatcgtgat taaagatgaa
    21061 gatttatcca attcgaattt tgcaggttgc agactacaaa acgctatttt ccaggactgt
    21121 aatatgtata aaacgaattt ttattacgcc ataatggaaa aaatactttt tgataattgt
    21181 attctcgatg actcaaattt cgctcagata aaaatggccg acggaactct aaatgcatgc
    21241 tccgctatgc atgttcaatt ctacaatgca gcaatgaata gagccaatat taaaaatacc
    21301 ttccttgact attcaaattt ttatatggcg tacatggctg aggtaaatct ttataaagta
    21361 atagcgccat atgttaattt atttaaagcc gaccttagtt tctctaaact cgatttaatt
    21421 aactttgaac atgctgatct gtctcgcgtc aatctgaaca aagcaatcct ccagaatata
    21481 aacttaattg atagcaaact cttttgtacg tggctgacaa atacattcct cgaaatggtt
    21541 atatgtaccg gctctaatat ggctaatgtt aattttaata atgccaattt aagcaactgc
    21601 catttcaact gttctatttt aacaaaagcc tgtatgttta atacccgtct ctatcgggtt
    21661 aatttcgatg aggctagcgt ccagggaatg ggcatttcca ttctccgtgg ggaggaaaat
    21721 atccccattg atagtgatac cctggtaaca ctacagaaat tctttgaaga agattgtacc
    21781 tctcatactg gcatgtcaca aactgaggat aatattaatg cagtcgctat gaagattact
    21841 gcagatatta tgcaacacgc agattgaggt taagtcgaag aacagccaat gaattgactt
    21901 tacaagagag ataacttatt tgttttagaa tcatgaatgt tcgcccgagc taacctgtta
    21961 aaggtgcaaa agattgcacc tttttcagat tacctcgggc ctggttacgt cgctttcggc
    22021 ttactgattt tcctcaatta gcaacgcttc aagcaaatcg agatcgtgca gtaatttttg
    22081 cagcgtttcg ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga
    22141 acgcaatgcc gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac
    22201 gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg acgcgagaac tgacctccgt
    22261 aagcaactgg ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg
    22321 gatcgccaca atcgccactt ctgccgttgc cgctcgcgca atacgttctt ctttcaattg
    22381 ttgcgaatgg tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac
    22441 aaacagcgag aagagaatga cgccagccgc caggaacacc agctcatagc gcgccgggaa
    22501 gacgttacca tccggcaaga gcagcgggat tgagagcaca ccggccagag tgatcgcccc
    22561 acgcacccca gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat
    22621 cggcttcttc ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg
    22681 gaccagcatc agcgccgcat atatcagaat aatatcggta aacagcatcc agatttcgac
    22741 gttagggtcg atttctgccg ccatcagcga cgtctccaga atacccggca gttgcagacc
    22801 taacagcagg aacaccatgc cgttaaagac aaattccagc atcgcccagg tgctgtttgc
    22861 gcgcaggcgc attgccagtg gcgcacggcg catcacaccg gagcgggtga tggtcatccc
    22921 ggcggcaacc gcagcgagga tgcccgaaac gccaatatgt tcggcaatca gataagaagc
    22981 gaatggcagc aagaacagca gaacgatctg cgtcgcgggt tcatcaccgc cccagcggct
    23041 gaggaatcgc agcgagcgac catacagcca gctcaccaca aaaccggcaa gaataccgcc
    23101 aatggctact ttcataaatt cgaccgtcgc accgccgaca gtgaagatca tcgtccccat
    23161 cgccactgcc acggcaaact tcaacgacac caggccggaa gcgtcattca tcaacgcttc
    23221 gccctgcaaa atgcccatga tttttttcgg gatgcgccct tcgccaacaa tcccggagag
    23281 cgccacagca tcggtcggag aaagtaccgc cgccagcgca aaggcgggga tcagcggaat
    23341 gcccggcacc acccagtaaa taaggaagcc aatgccgacc acggtgacca ccaccagcgc
    23401 cagcgcgagg ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca
    23461 gccatcagcg aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc
    23521 cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg
    23581 aagcgggatc tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt
    23641 cattatcagt atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat
    23701 ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
    23761 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga
    23821 ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc
    23881 gatgttcaac ttgcgccatt caccggaaac cagacgacca atcaccagag tcgcgaagcc
    23941 gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
    24001 cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag
    24061 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag aaagcggaga
    24121 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaacc cggttttacc
    24181 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag
    24241 gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccgccgt cgatgatctg
    24301 cccatcttta tccagcaggt tcgcctggcc ggcgacagcg cggatagttc cggtggcatc
    24361 aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
    24421 cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc
    24481 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
    24541 aatggtcagc agaatgccac cagggacttt cagtttttcc aagccgatga tcaccgccag
    24601 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
    24661 atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca gcaggaacag
    24721 accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
    24781 acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag
    24841 cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa acgccgtcag
    24901 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
    24961 gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc
    25021 caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc
    25081 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
    25141 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
    25201 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
    25261 gcggcaattt ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
    25321 gtaaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg
    25381 cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat
    25441 cgtcgatgtg caagattgca gcgttatcga gccgtatacc gttctggatt gcgataaaaa
    25501 atcgtctgag atgacaaaaa tattgcggct aaaaagtata cttttacact ggcgaagaaa
    25561 cgtcggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat
    25621 gaggtgtgtt gacgtcgggg gaaaccctcc tgtgttccag cgggatagag agaaagacaa
    25681 agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgtgttt gcggataatt
    25741 ccaaaagagc tcgatcagtc ccctcgcccc ttcggggaga gggttagggt taggggaaca
    25801 ggcagcacca gtgcaaatat ttaccctcac cccggcccca tagttaacga tgagtcagct
    25861 tgtccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgccgg taccttcttc
    25921 tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc
    25981 aatacatccg tccagttcgt cacgcagcgc cactaaggta tgaatgcgcc gatccaactc
    26041 ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaacgtat gcccttctgg
    26101 caacacgcca aacgcttcac caatggtcgc cagcggaatg ccaatacgct gagcgatttt
    26161 gataattgcg acatatcgca acacatcacg tttatatcgc cgctgattgc cgctgttacg
    26221 gatactagta atcaaccctt tgctttcata gaaatgcagc gccgataccg ccacaccgct
    26281 gcgtttcgcc acttcgccag gggttagcag cgctttaatg cggggtaatt tcttttccat
    26341 aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg aattaacgaa
    26401 ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg atcttatcgc
    26461 atggattgac gtgcatattg accagccgct taacattgat gtcgtcgcaa aaaaatcagg
    26521 ctattcaaag tggtacttgc aacggatgtt ccgcacggtg acgcatcaga cgcttggcga
    26581 ttacattcgc caacgccgcc tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc
    26641 gatttttgat atcgcaatgg acctgggtta tgtctcgcag cagaccttct cccgcgtttt
    26701 ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt tttattgccc
    26761 gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct gcggcggcat
    26821 cgccttcgca aaaaaccatc cctggcaata gcgcacgccg cgtttgcgca gccagttaac
    26881 ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccaggctgt gcgccagctc
    26941 gatgatgtgt tccgcaatca aatgactggt tttgtgggtg gtcagcgttt caacaaacga
    27001 tttatcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt tagagtagcc
    27061 aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca gaattaatcg
    27121 gcgtcatttt ttcgacatca agaaatgcat gctcagtcac ttcaaattta atttgctgcg
    27181 gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt gacgtatgaa
    27241 aatcggaggc cgacaggtta atagaaacat agcgatcggc atgtgttgcc aggtacgcgc
    27301 ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttctatc atcccctcct
    27361 tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca ccaggccaac
    27421 gtaacaacgc ttcagcgccg atacattttt ctgttttgat atcgattatt ggctggtaat
    27481 aaagacaaag ttgatgtttt tcgagggcgc gttgcaattt acgtttggga gataaatagt
    27541 tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata ccggcgggca
    27601 acgcaaaaat aagatgatta taaaaatggg ttataagacg ttgatatgaa gtcgcaacaa
    27661 tggctgcaat tgggcgtttt gtcgaataaa ttgtcgcata taaatagcca tttctttgta
    27721 cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc tctttgctta
    27781 acgagaaaaa ggttttcgtc accgtatcat acacacccca ttgcaatgtc gggtcatcag
    27841 acatcacttc actccagaag agagggttga taaccgccac ataatttccc cgctgcatat
    27901 aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata gaaacgttag
    27961 gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct atcagcgatg
    28021 agcataaaaa atggttatca cgaattcatt aatataaaga tagccacgaa taatattcaa
    28081 cattcgcttt tgatgggctg gagtgcaaac ttgcccctga tagcgttcag ctgcatcgct
    28141 cactaaatct gcctggcgaa tgaccagctc ggatttatcc agtgccagtt gagcaaaggt
    28201 tcggagctga ttattcactt ctgattttgc ccaaaggaag gcaatccata gcgaaagaat
    28261 gatgggaaag agaactaaaa agataatgcc cggcaacgcc agtaattggt gtcgtgcacg
    28321 atgactcatt gctatcaatt tcctgcctgt ttatacccag ccattgcaag gtgaaaccct
    28381 ggctaccgac gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa
    28441 ttgatacctt tttaatcgtc acctggatga aaataattca ttgatattca aaattaattt
    28501 tctacaggaa tacagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata
    28561 atttgtacag caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata
    28621 gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga
    28681 tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt
    28741 ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcaac
    28801 ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt cgtcggtctg
    28861 attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg
    28921 caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac aacatcgagt
    28981 cggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt ttgacctgat
    29041 acgtagaa
//
LOCUS       DAECNO010000047        28588 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.047, whole genome shotgun sequence.
ACCESSION   DAECNO010000047 DAECNO010000000
VERSION     DAECNO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.047"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(24..278)
                     /locus_tag="KKO08_002110"
     CDS             complement(24..278)
                     /locus_tag="KKO08_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBD6318350.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="KKO08_002111"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="KKO08_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBD6318351.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="KKO08_002112"
     CDS             1228..2694
                     /locus_tag="KKO08_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD6318352.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="KKO08_002113"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="KKO08_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBD6318353.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="KKO08_002114"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="KKO08_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBD6318354.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="KKO08_002115"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="KKO08_002115"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBD6318355.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="KKO08_002116"
     CDS             5709..7265
                     /locus_tag="KKO08_002116"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBD6318356.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="KKO08_002117"
     CDS             7347..9161
                     /locus_tag="KKO08_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBD6318357.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="KKO08_002118"
     CDS             9162..10256
                     /locus_tag="KKO08_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBD6318358.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="KKO08_002119"
     CDS             10256..11281
                     /locus_tag="KKO08_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6318359.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="KKO08_002120"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="KKO08_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBD6318360.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="KKO08_002121"
     CDS             complement(12876..13220)
                     /locus_tag="KKO08_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318361.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KKO08_002122"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="KKO08_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBD6318362.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KKO08_002123"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="KKO08_002123"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBD6318363.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="KKO08_002124"
     CDS             15616..17376
                     /locus_tag="KKO08_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBD6318364.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="KKO08_002125"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="KKO08_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBD6318365.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KKO08_002126"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="KKO08_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBD6318366.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="KKO08_002127"
     CDS             19113..19340
                     /locus_tag="KKO08_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBD6318367.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="KKO08_002128"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="KKO08_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBD6318368.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="KKO08_002129"
     tRNA            21195..21271
                     /locus_tag="KKO08_002129"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            21478..22722
                     /locus_tag="KKO08_002130"
     CDS             21478..22722
                     /locus_tag="KKO08_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6318369.1"
                     /translation="MARKATPLTTTQIKAAKPAEKEYTLQDGGGLFLVIKPSGSKLWR
                     FNYYRPSNKKRTLISLGSLDEVSLADARKRRSEYRTLIAAGTDPQEYERQKREAEARR
                     QGNTFENVAALWYEMKKNQNLAHNTIKDIWRSLEKYVFPYIGNTPIDTLTARRFVEIL
                     TPIKARGNLETLKRVLQRINEVMDFAANSGMIDINTAANVRKTFPSPTKKHMPTIRPE
                     QLPQLMHDLSIASIERQTRLLIEWQLLTATRPAEASAARWEEINLEAATWTIPAGRMK
                     MRRDHVIPLCAQAMAVLEAMKPISARREHVFPSLKNPVQPMSSQTANAALRRMGYTGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYLEKRKVLMCWWGD
                     FVEAAATGTTIASGHRGLRGIK"
     gene            22997..23188
                     /locus_tag="KKO08_002131"
     CDS             22997..23188
                     /locus_tag="KKO08_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBD6318370.1"
                     /translation="MRAILLRPEVKALLRIKSDSTLTKMVNKGGFPKGFRVGLRRIGW
                     YEDEVLAWLKERERESREA"
     gene            23415..23858
                     /locus_tag="KKO08_002132"
                     /pseudo
     CDS             23415..23858
                     /locus_tag="KKO08_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            23834..24022
                     /locus_tag="KKO08_002133"
     CDS             23834..24022
                     /locus_tag="KKO08_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBD6318371.1"
                     /translation="MATIPALAHTQNAFIWRFIIVNPSDYRVVHVVASTEREARNYCP
                     SGCVAVFAARIRQGVSHA"
     gene            24015..24200
                     /locus_tag="KKO08_002134"
     CDS             24015..24200
                     /locus_tag="KKO08_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318372.1"
                     /translation="MLKRFQITARAVNHQGHVIDIRQNVNATSVNAAIAILKEELIGC
                     GFTQVMIRAVVEVSTVH"
     gene            24240..24539
                     /locus_tag="KKO08_002135"
     CDS             24240..24539
                     /locus_tag="KKO08_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBD6318373.1"
                     /translation="MKVKNSGFTASGPVRPEIHTGDIFFDKYGWMVKVISVEERRITC
                     RREGYNYDCVTPVYQFRRDFSLMKAAPRSKPTSREKERGNIQKMKSMINTFRGKK"
     gene            24536..26671
                     /locus_tag="KKO08_002136"
     CDS             24536..26671
                     /locus_tag="KKO08_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBD6318374.1"
                     /translation="MKRAPNLKHQPADKMTEVIIFAGSDAWAHAKQWQEMDGRLAGDN
                     VPPVWLGDQQLAELENLNIIDDGRYCVRLYKAGHIKPSNINTIAQKLAAAGIRDANYY
                     PDGMHSQKRENWREYLERERQNIDDGLAIRLPTKENATGSHGDDELKPRVESRADGVF
                     WVTPKVDKQTGEIIRPETWLCSPLELLGTGTIGREHHRVMRWKKTANHEVITMAVPCG
                     GIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGNHEEWQLSTTTGWHFGAYIMPD
                     GSVIGESEKPILFTGKTAAVNGYSVAGTTEGWRDTVARLAGGNPSMMLGVAVSLSAPL
                     IGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEAHND
                     GLLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEMDVE
                     TFLKSEGIKVKAGQLVRLLNVPMEKSTQFHEYSTGKAHADALKDAWTANHGAAGREWI
                     KWLAAHQQKAKDTVRACRERWRNLIPESYGEQVHRVGERFAILEAALVLSGNVTGWDV
                     QACRDAIQHNFNAWVKEFGTGNKEHRQIIEQAEAFLAAYGMSRFAPVNYDPASLPISE
                     LYGYRESDGRYGESVLFYVLPEPFKSHVAKGFNKDAVARVLHEAGMLKKPASGEGWQI
                     RTPRLKHMKGARLRVYGLLLAQDHDDEGG"
     gene            26908..27117
                     /locus_tag="KKO08_002137"
     CDS             26908..27117
                     /locus_tag="KKO08_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBD6318375.1"
                     /translation="MTAQISAYGRLVADPQLKTTSKGTPMALVSMAVPIPCNQADDGQ
                     ATMWLSVLAFGRQADALAKHCKYPA"
     gene            27179..28290
                     /locus_tag="KKO08_002138"
     CDS             join(27179..27440,27440..28290)
                     /locus_tag="KKO08_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6318376.1"
                     /translation="MRKARFTEHQIIAVIKSVEAGRTVKDVCREAGISEATYYNWKSR
                     YGGMEASDIKKIKELEDENRRLKQMFADLSLENRALKDVIEKKPLKPACKRELVTHLI
                     TTFGLSIRQACRSLNLSRTVYHYRPDTTRDEPVIVALQAVAERYPRYGFPKLFQVLRR
                     QGYPWNHKRIHRIYCLLKLNFRRKGKQRLPVRNPSPLATSEALNQSWSVDFMHDALVC
                     GRRFRTFNVVDDFNREALSIEIDLNLPALRVVRVLDRIAANRGYPVKLRMDNGPEFIS
                     LTLAEWAEKHAVKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEVREITEKW
                     LSEYNCERPHESLNNMTPEEYRQHHYLAGISKNAWN"
BASE COUNT         7270 a         7085 c         7200 g         7033 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaatcaacta tagctaatca tcgtttttat
    21301 gaaaaaagcc cgctaattca ttgaatagcg gtttttttta ttgtttttag tcatcgttat
    21361 tcgacggtaa cagtggtttt cagccgtttt ttttagttat atgtttagtt atacgggctg
    21421 gcgcatagct aaatccgtat aactagaacc tgtttttccc cgtggattca taaacgtatg
    21481 gcaagaaagg caaccccact caccaccaca cagatcaaag cagctaaacc agcagaaaaa
    21541 gaatacacct tacaggatgg cggcgggctt tttctcgtta ttaagccgtc tggttcgaaa
    21601 ctatggcgat tcaactacta tcgaccttcg aacaaaaaac gaacactcat tagtttggga
    21661 tcgcttgatg aagtctccct tgctgatgcc agaaaacgcc gtagcgagta caggacgtta
    21721 attgcggcag gaaccgaccc gcaggaatac gaacggcaaa aacgcgaagc agaggctcga
    21781 cgacagggca acacgttcga aaatgtggcc gcattgtggt acgaaatgaa aaaaaaccag
    21841 aatctcgccc acaatacgat caaggacatc tggcgctcgc tagagaaata tgtattcccg
    21901 tatatcggaa acacaccgat agatactctc accgctcgcc gctttgttga gatactcacg
    21961 cccatcaagg cacgtggcaa cctggaaaca ctgaaacgcg ttttacagcg catcaatgaa
    22021 gtgatggatt ttgctgccaa cagtggaatg attgacatca acaccgccgc gaacgttcgc
    22081 aagacattcc cttctcccac caaaaagcac atgccaacca tccggccgga acaactaccg
    22141 cagctaatgc acgatttatc gatcgccagc atagaacggc aaaccagatt gctgattgag
    22201 tggcagttgt taaccgcaac ccgcccagcc gaagcctctg ccgcacggtg ggaagaaatc
    22261 aaccttgagg cggcaacctg gacgatacca gccggacgca tgaagatgcg ccgtgatcat
    22321 gtgatccccc tttgcgctca ggcgatggca gtgcttgaag ccatgaagcc gattagcgca
    22381 cgaagggagc atgttttccc aagtcttaaa aatccagtgc aaccaatgag cagccagaca
    22441 gcaaacgcag cattacggcg aatgggttac actggtgtgc tggtatctca cggactacgc
    22501 gccatattca gcacagcggc gaacgaagaa ggatttgagc cggacgtaat cgaggccgca
    22561 ctcgcacacg tggacacgaa cgaagttaga cgggcataca accgaagcaa ctacctggaa
    22621 aaacgtaaag tgttaatgtg ctggtggggt gattttgtgg aagcagccgc aaccggaaca
    22681 accatcgcca gcgggcacag aggattacgc ggaataaaat aaaatattaa aaaaattatt
    22741 agctcctaat tggtatcagt taggagccta tttggagcta atttaaattt catattttta
    22801 tgttagaaaa ttaattttaa atttctaacg ccattatcta aagttagaag ccccattatt
    22861 taaagttaga agaaaaataa ttattttttg ctaaatggct cttgacttat ttttttatga
    22921 ttttgtatgc ttattacatc acttaccgtt attttcattc aaaaagcgat attttgctgt
    22981 ccagaggaaa aataagatga gggctattct tttacgacca gaggtaaagg ctttgctacg
    23041 cattaaatct gattcgaccc taacgaagat ggttaacaaa ggcgggttcc ccaaaggatt
    23101 ccgtgtcggg ctgcgtcgca ttggctggta tgaagatgag gtactcgcct ggttgaaaga
    23161 acgtgagagg gaaagtcgtg aagcgtaagt gtgattaacg ttcgcctgat tactatcaag
    23221 taataagaca attaagagag aacattatga atactggcaa aaatgcccaa gcaggggcag
    23281 gttttcgttc aaccataaaa aatatggctg atgttattat tcagttcaaa ccttacaaaa
    23341 gaatcaattg aactaatcgc acttccgttt taaacggaaa cgtacaatcg catagcttaa
    23401 acgacaaaag aaatatgctt tttacaaata aaataaaggc gaccgcagag ggccgccaga
    23461 ggacatgtat taaacttgaa cgcatcgcca acaatgccat atttacagct ggtgggcaat
    23521 gtgatttgtg tggtgggcac accttcaaca catccgacca catgggccgg aaaacgggat
    23581 atactcgccg aatcccttat aaaacgggga tcgggattac caccccgctg aagcaaaagg
    23641 cgacacatga cgcgccagcg tcttttttgt tgtctgtaca cacgcacacc ttatcaatgg
    23701 tgggctgtat ggggctgact tcggtcaggc tggtctcctt ttgcgccagt tgtggtaacc
    23761 ctgtacagtc caccgccagc gagcttacca cctcctgcgg tgggtatatc ccaacgcaaa
    23821 aggaggctgc caaatggcta ctatccctgc cctcgcacat actcagaacg cctttatctg
    23881 gcgctttatc atcgttaatc cgagtgatta ccgcgtcgtt cacgtcgtgg cgtcaaccga
    23941 acgagaagcg cgtaactatt gcccgtccgg ttgtgttgcc gtattcgctg cccgtattcg
    24001 tcagggggta agtcatgctt aagcgattcc agataacagc acgcgctgta aatcatcaag
    24061 gccacgttat tgatattcgg caaaacgtga acgccaccag cgttaatgca gctatagcaa
    24121 tattgaaaga agagttaatc ggctgtgggt tcacgcaggt gatgatccgt gccgtagttg
    24181 aagtgtcgac tgttcattaa tctcattaat acagctttaa caaaaacgta attcgacaaa
    24241 tgaaagtaaa aaattctggc tttactgcca gcggccccgt tcggcctgaa atccacaccg
    24301 gcgatatttt cttcgataaa tacggctgga tggtaaaggt gataagcgtg gaggaacggc
    24361 gcatcacctg ccgtcgtgaa ggatataact acgactgtgt aacgcctgta tatcagttcc
    24421 ggcgtgattt ctccctgatg aaggccgcgc cccgcagtaa acctactagc agggaaaaag
    24481 agcggggaaa tatccagaaa atgaaaagca tgattaacac attcaggggc aagaaatgaa
    24541 acgtgcaccg aatttaaagc atcagccagc cgacaaaatg acggaggtta tcatttttgc
    24601 gggtagtgat gcctgggcac atgcgaagca gtggcaggag atggacgggc gacttgctgg
    24661 cgataacgtg cctcctgtct ggcttggaga tcagcaactg gcagaactgg aaaatctgaa
    24721 catcattgac gacgggcgct attgtgtccg gctgtacaag gcagggcaca tcaagccatc
    24781 aaacatcaat accattgcgc aaaagctggc ggcggcaggc atacgggatg cgaattatta
    24841 ccccgacggg atgcacagcc agaagcggga gaactggcgc gaatatctgg aacgtgagcg
    24901 ccaaaacatc gatgacgggc tggctatccg gcttccgaca aaagaaaacg ccaccggaag
    24961 tcatggcgat gatgaattaa agccccgcgt tgaaagccgc gcagatggtg ttttctgggt
    25021 aacacccaaa gtggacaagc agacaggcga gataatcaga cctgaaacgt ggttatgttc
    25081 cccgcttgaa ctactgggaa cggggacgat cggcagggag catcaccgcg tgatgcgctg
    25141 gaaaaaaacg gcaaatcatg aagtcatcac gatggcggtt ccgtgcggtg gcattggcga
    25201 ccgtgacggc tggcggttgt tgaaagacca cggccttaac gtgacaacca acggcaaata
    25261 cagagcaata ctggctgact ggatgcagtt aagcggaaac catgaggaat ggcagttaag
    25321 cacaacaacg gggtggcatt ttggcgcgta catcatgccg gacggttcag tcattggtga
    25381 gtctgaaaaa ccgatcctgt ttaccggaaa aacggctgcc gttaatggtt attccgtagc
    25441 aggaacgacg gagggctggc gcgatacagt ggcgcggttg gctggtggca atccgtccat
    25501 gatgctgggg gtggcggtat cgttatcagc gccattaatc gggctggtgg gcgctgacgg
    25561 tttcggtgtg catcttttcg aacaatcatc ggcaggtaag accaccacgc agaacatcgc
    25621 atccagtttg tggggagagc cggacgcaca acggctgacc tggtacggaa cggcgttagg
    25681 tatcgccaac gaggccgaag cacacaatga cgggctgtta cccctggatg aaataggcca
    25741 agccggaaac gcacgggagg tgtccacgtc ggcttatacg ctgtttaacg gttcgggaaa
    25801 attacagggg gcgaaggacg gcggcaacag ggagataaaa cactggcgaa cagtggcaat
    25861 cagtaccgga gaaatggacg ttgaaacatt cctcaaaagt gaggggataa aagtcaaagc
    25921 ggggcagctt gtccggttgc ttaacgtgcc gatggagaag tccacgcagt ttcacgaata
    25981 cagcacagga aaggcgcacg cagacgcgtt aaaggatgcc tggacagcaa atcacggggc
    26041 ggcaggtcgt gagtggatta aatggctagc agcccaccag cagaaggcta aggacacggt
    26101 aagggcatgc cgtgaacggt ggcgcaacct gataccggag agctacggcg aacaggttca
    26161 ccgtgtaggg gaacgattcg ccatactgga ggccgcgctt gtgctgtcag gcaatgttac
    26221 gggttgggat gttcaggcgt gccgtgatgc cattcagcat aactttaatg cctgggtgaa
    26281 ggagttcggc acgggtaaca aggaacacag gcagattatt gaacaggcag aggcgtttct
    26341 tgctgcctac ggcatgagca ggtttgcgcc tgtcaattat gaccctgcaa gcctgccgat
    26401 ttcagaactg tacggctaca gggaaagcga cgggcgttac ggtgagtctg ttttgtttta
    26461 cgtcctgccg gagccattta aatcgcatgt ggcgaaggga tttaacaagg atgccgtagc
    26521 aagagtcctt cacgaagctg gaatgctgaa aaagccagcc agcggggaag gatggcaaat
    26581 cagaacacca cgtcttaaac acatgaaggg ggcacgacta agggtgtacg gccttttact
    26641 tgcacaggat catgatgatg aaggaggtta atctctcgcg tgtaggttta aaaacacttg
    26701 tcccacctgt cccaacctgt gggaaacaca cataacacac tgaattatat tgggtaacat
    26761 cagaaaaagt gggacaagtg tgggacaagt taaggtgatt ttgggacaag ttaggagcat
    26821 tttgggacaa gtcagcaggc gttatttttt gcctggctgg tggacattaa aaatccaacc
    26881 aacatttaaa accggagatc agacaatatg acggcacaaa tatcagctta cgggcggctg
    26941 gtagctgacc cgcagttaaa gaccaccagc aagggaacac caatggcgct ggtgagtatg
    27001 gctgttccca ttccgtgcaa tcaggcagat gacggacagg cgacgatgtg gttatccgtc
    27061 ctggcatttg gcagacaggc cgacgcactg gcaaaacact gtaagtatcc cgcataatcg
    27121 tgccattcac atttagagat cctccggcat actcaatctg ccaattaagg agatcgctat
    27181 gcgtaaagcc cgttttactg agcatcagat catcgccgtg attaagtcgg ttgaagccgg
    27241 acgaactgtt aaagatgtct gccgggaggc cggtatctct gaagccacct actacaactg
    27301 gaagtccaga tacggtggca tggaggcttc tgatattaaa aagattaaag agcttgagga
    27361 cgagaaccgc cgtctcaaac agatgtttgc cgacctgagc ctggagaacc gggcgctgaa
    27421 ggacgttatc gaaaaaaagc tttaaaacca gcctgtaagc gtgagctggt cactcatctg
    27481 ataacgacat tcggactcag tatccgtcag gcctgccgga gtctgaacct gagcagaacg
    27541 gtttaccatt accgcccgga taccacgcgt gatgaacccg ttattgtcgc gttgcaggca
    27601 gtggcagagc gatacccacg atacggtttt ccgaaactct tccaggttct gcggcgacag
    27661 ggatacccat ggaatcacaa aaggatccac cgtatttact gtctgctgaa gctgaatttt
    27721 cgccgtaagg gcaaacaacg gttgccggtg cgtaatccct cgccactggc cacgtcggaa
    27781 gcgctgaacc agagttggtc tgtcgatttt atgcatgatg ccctggtctg tggccgtcgt
    27841 tttcgtacgt tcaatgtcgt tgatgacttt aaccgtgagg cgttgtcgat tgaaatcgac
    27901 ctgaatctgc cagctctacg cgtggtccgt gtactcgaca ggatcgcggc aaaccgtggc
    27961 tatccggtca agctgcgcat ggataatggt ccggaattta tctccctgac actggctgaa
    28021 tgggcagaga aacatgcagt aaaactggag tttatccagc cgggtaagcc gacgcagaac
    28081 gctttcattg agcgctttaa ccggacatac cgtacagaaa tactcgattt ttatctgttc
    28141 agaacgctga atgaagtgcg ggaaatcacg gaaaaatggt tgtcagaata taactgtgaa
    28201 cgcccgcatg aatcactgaa caatatgacg ccagaggaat atcgacaaca ccattatttg
    28261 gccgggatct caaaaaatgc ctggaactaa aacgggtcta tttacacccg taagccaaca
    28321 atagctcgta gataaaatca tcttttgaga tttcttggct tgatacttgt tttaccaagc
    28381 tcttaacatt ttcttctatt tgtacgctat tcatgttttt tactgctcaa atgacattgg
    28441 ttgcattgta tattcaacgt ttgtaatgaa ccatgagaac acctacagtt ttttacattt
    28501 tttgtgtctt ttggctctta ccgtctaata tcctaggcta ggaggtataa tcgcaggttt
    28561 acgtttattg ttgcagcgat ttatattg
//
LOCUS       DAECNO010000048        28434 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.048, whole genome shotgun sequence.
ACCESSION   DAECNO010000048 DAECNO010000000
VERSION     DAECNO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.048"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            564..1334
                     /locus_tag="KKO08_002139"
     CDS             564..1334
                     /locus_tag="KKO08_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBD6318377.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="KKO08_002140"
     CDS             1325..1798
                     /locus_tag="KKO08_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBD6318378.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="KKO08_002141"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="KKO08_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBD6318379.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="KKO08_002142"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="KKO08_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBD6318380.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="KKO08_002143"
     CDS             3233..3727
                     /locus_tag="KKO08_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBD6318381.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="KKO08_002144"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="KKO08_002144"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBD6318382.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KKO08_002145"
                     /pseudo
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="KKO08_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7571..8485
                     /locus_tag="KKO08_002146"
     CDS             7571..8485
                     /locus_tag="KKO08_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBD6318383.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="KKO08_002147"
     CDS             8700..10061
                     /locus_tag="KKO08_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6318384.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="KKO08_002148"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="KKO08_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBD6318385.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="KKO08_002149"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="KKO08_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBD6318386.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="KKO08_002150"
     CDS             12611..13900
                     /locus_tag="KKO08_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBD6318387.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="KKO08_002151"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="KKO08_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBD6318388.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16216
                     /gene="hpaD"
                     /locus_tag="KKO08_002152"
     CDS             15365..16216
                     /gene="hpaD"
                     /locus_tag="KKO08_002152"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBD6318389.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16226..16606
                     /locus_tag="KKO08_002153"
     CDS             16226..16606
                     /locus_tag="KKO08_002153"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBD6318390.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16674..17477
                     /gene="hpaH"
                     /locus_tag="KKO08_002154"
     CDS             16674..17477
                     /gene="hpaH"
                     /locus_tag="KKO08_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBD6318391.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17488..18291
                     /gene="hpaI"
                     /locus_tag="KKO08_002155"
     CDS             17488..18291
                     /gene="hpaI"
                     /locus_tag="KKO08_002155"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBD6318392.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18313..19668
                     /gene="hpaX"
                     /locus_tag="KKO08_002156"
     CDS             18313..19668
                     /gene="hpaX"
                     /locus_tag="KKO08_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBD6318393.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19678..20568
                     /gene="hpaA"
                     /locus_tag="KKO08_002157"
     CDS             19678..20568
                     /gene="hpaA"
                     /locus_tag="KKO08_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBD6318394.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20595..21532
                     /locus_tag="KKO08_002158"
                     /pseudo
     CDS             20595..21532
                     /locus_tag="KKO08_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21759..23317
                     /gene="hpaB"
                     /locus_tag="KKO08_002159"
                     /pseudo
     CDS             21759..23317
                     /gene="hpaB"
                     /locus_tag="KKO08_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23335..23847
                     /locus_tag="KKO08_002160"
     CDS             23335..23847
                     /locus_tag="KKO08_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBD6318395.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24240..26390
                     /gene="btsT"
                     /locus_tag="KKO08_002161"
     CDS             24240..26390
                     /gene="btsT"
                     /locus_tag="KKO08_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBD6318396.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26440..26643
                     /locus_tag="KKO08_002162"
     CDS             26440..26643
                     /locus_tag="KKO08_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBD6318397.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26654..27610
                     /gene="yjiA"
                     /locus_tag="KKO08_002163"
     CDS             26654..27610
                     /gene="yjiA"
                     /locus_tag="KKO08_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBD6318398.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27679..>28434)
                     /locus_tag="KKO08_002164"
     CDS             complement(27679..>28434)
                     /locus_tag="KKO08_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBD6318399.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6961 a         7432 c         7127 g         6914 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15841 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15901 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15961 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
    16021 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    16081 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    16141 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
    16201 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
    16261 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
    16321 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
    16381 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
    16441 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgatggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
    17221 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
    17281 tcgccgctgg tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg
    17341 caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc
    17401 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta
    17461 gctgccgctt tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca
    17521 ggccgtccgc agattggatt atggctgggg ctgagcagca gctacagcgc ggagttactg
    17581 gccggaccag gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa
    17641 accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt ggtacgccca
    17701 tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca aaccttactg
    17761 gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc
    17821 cccgccagta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt
    17881 cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt
    17941 gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc
    18001 ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca cccggaagta
    18061 caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc
    18121 ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc
    18181 gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt
    18241 aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta aacgtacgat
    18301 gtggagcgca tcatgacgac ctctaccctg caatataaag ataataaaac tgttgaaatt
    18361 gaacatcgtg tcattaataa attatttcgt cgcctaatca tttttctttt tattctgttt
    18421 gttttttcgt ttcttgatcg catcaatata ggatttgccg gactgacaat gggcaaggat
    18481 cttggcctga cgtccacaat gtttggcctc gccgcaacac tcttttatgt cacgtacgtc
    18541 atgtgcggga tccccagtaa tatgatgtta gcgaaaattg gcgctcgtcg atggatcgct
    18601 gggatcatgg tggtctgggg aattgcttcc acctgcacaa tgtttgcaac aagccccgaa
    18661 acactctatc tactgcgtat gctggttggt attgctgagg cgggtttttt acctggtatt
    18721 ttggtttatc tgacctggtg gttcccggca tatcatcggg ccagagccaa tgccttattt
    18781 atgatagcga tgccagtcac tatgatgctg ggattgatcc tttcaggcta cattctggcc
    18841 ttggatggac tgtggaatct aaaaggctgg caatggctct ttttgctgga aggtctgcca
    18901 tcggtcattc ttggtgtcgt cacctggttt tatctcaacg acacaccaga ccaggcgacc
    18961 tggcttgatg aaaacgaaaa acaggcttta aaaacgatga ttctccgtga acaagagagt
    19021 gcatttgccc atgcatctgt tccacgctca accttacgtg aagtcctgac cccagccatc
    19081 atgctatata cacttgctta tttttgcctg actaatactc tgagtgcgat taatatctgg
    19141 acgccgcaaa tcctacagag tttcaatact ggcagtagca acatcatgat tggtttgctg
    19201 gcggccatcc cccaattctg cacaattttt ggcatgatct ggtggagtcg tcgttccgac
    19261 cgtcttaaag aacgcaaaaa gcatacgatt cttccctacc tgtttgccgc tgccggatgg
    19321 ttactcgctt ctgcgacaga tcacagcctc attcaactga gcagcatcat tatggcctca
    19381 atgggatcat ttaccgcgat gactatcttt tggacaacac cggatcaagt tataagcttg
    19441 caatcgcgtg ccgttgcgat ggctgtcatc aatgctgtcg gcaatgtagg ttcagcagtc
    19501 agtccattac ttatcggtat tttgaaagat gcaaccggca gttttagctc aggtttgtgg
    19561 tttgtagcag gattgctggt actgggcgcg ttagtcatga cccgaatccc tatggggagt
    19621 cgggaacact ctctttcatc tgaacttatc gcaccaaaac cgcaataagg agtcatgatg
    19681 tccagtgaag ctatctttga acatggttat gaaaatattg atttggtccg ggaatatgat
    19741 gcccgctacg ctggtgaaga tgtgcattat gaaacatttg cccggttagc gaatttcttt
    19801 ggacgcgata tgcgccctca ctggcacgat agatattttc aactccatta tctggtcact
    19861 ggacgcatta ctttacaact ggatgagcac ttctattctg tgcgcgcacc gctttttgta
    19921 ctaacaccac catcggtgcc acatactttc tttactgatc ctgatagcga tgggcatgtt
    19981 ctgaccgtac gtcaggagtt aatttggccc ctggtagaaa agctctggcc tggtagtgga
    20041 gatgccgcaa tgctacaagg tatctgtctg tcactggagg gaatggaaca tacacaggcg
    20101 gcattaaatc attattggcc gataatatcc ctggagtttc aacaaaataa gcaaggacgg
    20161 gaaattctat tgcaatcact ggcgcaaagt atttttacaa tcctactgcg tgaagcacca
    20221 ccaaatgata tttcaacatg cagcgtgcgc ggcgagatgt gtttattcca gaagtttaat
    20281 cggctgattg acgaaaaata tcgccaacat ttaatggtcc ctgaatatgc aacaatgctc
    20341 ggtatgagtg aatcacggct taccgagctg tgccgacgtt ttgccaatca atcgccgaag
    20401 agattgattt tcgaacgagt gagccgggaa gccagacggc ttctgctcta tagcgagcaa
    20461 agcatcaacc agatagccct tgatcttggc tacaaagatc ccgcctattt cgcccgtttt
    20521 ttcaaccgaa tgatgggttg cccaccaaca caattccgtg gtcgctgacc atttatcaac
    20581 ctgagagact cgacatgaaa atatctttac gctcactatt tgtggcgtgc ctgattgcgc
    20641 cagtggcatt cacctacgcc tccgaaccgc aataaatgaa aattgtacta cttggaacga
    20701 aaggtggccc ttctttatta aaagctgacc gtttaccgat ggcaacggca cttattattg
    20761 gcaataaaac ctagttaatt gatgctggtt acggggccag cttacaactg gtaaaaaata
    20821 atattccact gcgccagatt gataatattt tactcaccca tttacacagt gatcatattc
    20881 tggactatcc atcagtctta atgaatgcat gggctagcgg tctgcaggac aaaactattc
    20941 atgtattcgg tccgccaggc acccgagcca tgaccgctgc cagttggcag gtcttcgatc
    21001 gggacatcac actgcgtatg gaagaagaag gaaaacccga tcctcgcaaa ctcgttacag
    21061 ctcaagatgt tggtgaaggc gtagtttata aagatgatac cgtgaccgtg actgcattaa
    21121 aggtccctca ttctcctttc cctgacggtg aggcttttgc ttatcgcttc gacacacaag
    21181 gcaagcgaat tgtcttctca ggagatacct cgtactttcc cccactggca actttctcag
    21241 aaggcgctga tatacttgtg catgaggccg tacatgagcc ctccgtcgct aagctggcag
    21301 acagtattgg caatggtaaa acactggcaa aagccattgc ttctcaccat acgactattg
    21361 aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa actcatcctt agccacctgg
    21421 taccaatcac cgttccggat gaaacatgga agcaagaagc acagaaaact attcaggccc
    21481 tatcgtcgtt ggtcatgaaa atatgacgat taccattcca ttcgccccat aaaaaattcc
    21541 gtccccgtta gcccttgtta gcggggaata ttatgatttg ccttactgta agttgatcac
    21601 gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa gtaccaacgt tcgcctgaaa
    21661 cgtctcttcc cgacaaccgc gttcaacgct tcaattaaat aacaaaaaca acacacaaaa
    21721 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
    21781 ccagtaccca acgtcctttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
    21841 agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg
    21901 cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg caggactctc
    21961 tgtgttggaa caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga
    22021 aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgaatggtca cgcctgagct
    22081 atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga
    22141 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg
    22201 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgtcatt tgccgaccga
    22261 taaagtgaaa gacgtttaca tcaagctgga aaaagagact gacgccggga ttatcgtcag
    22321 cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga ttggcttcgg
    22381 ctcggcacaa gtgatgggcg aaaacccgga cttcgcactg atgttcgttg cgccaatgga
    22441 tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg
    22501 ctcgccatac gactacccgc tctccagccg cttcgatgag aacgatgcga ttctggtgat
    22561 ggataacgta ctgatcccat gggaaaacgt gctgatctac cgcgattttg atcgctgccg
    22621 tcgctggacg atggaaggcg gtttcgcccg tatgtatccg ctgcaagcct gtgtgcgcct
    22681 ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat gtaccggcac
    22741 cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc gcaacacctt
    22801 ctgggcattg agtgactcga tgtgttcaga agcgacgccg tgggtcaacg gggcttattt
    22861 accggatcat gccgcactgc aaacctatcg cgtactggca ccaatggcct acgcgaagat
    22921 caaaaacatt atagaacgca acgttaccag tggcctgatc tacctccctt ccagtgcccg
    22981 tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg gttcgaacgg
    23041 tatggatcac gtccagcgca tcaagatcct caaactgatg tgggacgcca ttggcagcga
    23101 atttggtggt cgtcacgagc tgtatgaaat caactactcc ggtagccagg atgagattcg
    23161 cctgcaatgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga tggcgatggt
    23221 tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc tgcacaacaa
    23281 cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt taagatgcaa
    23341 ttagatgaac aacgcctgcg ctttcatgac gcgatggcca gcctgtcggc agcggtaaat
    23401 attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc tgtctgctcg
    23461 gtcacggata caccaccgtc gctgatggtg tgcattaacg ccaacagtgc gatgaacccg
    23521 gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca ggaactgatg
    23581 gcgcgccact tcgcgggcat gacaggcatg gcgatggaag agcgttttag cctctcatgc
    23641 tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag tcttgaagga
    23701 gagatccgcg atgtgcaggc gattggcaca catctggtgt atctggtgga gattaaaaac
    23761 atcatcctca gtgcagaagg tcacggactt atttacttta aacgccgttt ccatccggtg
    23821 atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg tcgaaggggc
    23881 atttttattg tgatctcaat cacatatcta cccgatgaat aattcttgcc caaccgctca
    23941 ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac cttactttac
    24001 gcgcacgtat ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca
    24061 acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgga ttttggtttg
    24121 caggaaggca gcaagtgagt gaatccccgg gagcttacaa aagtaagtgt cagaggtgaa
    24181 cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca ggttttacta
    24241 tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc atcggtgcat
    24301 tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg
    24361 tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac atcgcccaga
    24421 aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga
    24481 actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg
    24541 ctggtccgct ggtgggaccg gttctcgccg cgcagatggg ctacctgcct ggcacgctgt
    24601 ggctgctggc gggggtagtg ctggccggtg cggtacagga ctttatggtg ctgtttatct
    24661 cctctcgccg taacggcgca tctctcggtg agatgatcaa agaagagatg gggccagtac
    24721 cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctc gccgttctgg
    24781 cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa
    24841 ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg gggcgtgtgg
    24901 gtgaagtctc tgttattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga
    24961 ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc attaccttcg
    25021 cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc
    25081 gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg
    25141 tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc
    25201 cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat
    25261 ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg
    25321 acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc
    25381 tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg
    25441 gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca
    25501 tggcgcagct gaaagacgtt accgcacacg cggcagcgac cgtcagctcc tggggcttcg
    25561 tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga
    25621 accgtgcagg tggtgcgccg acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc
    25681 tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca
    25741 tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg
    25801 gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg
    25861 cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg
    25921 gcgttaagag cctgtggccg ctgttcggta tctccaacca gatgctggca gccgtagcgc
    25981 tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg
    26041 ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg aaactgttca
    26101 gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg
    26161 ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca
    26221 actacaccaa cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct
    26281 acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac aaagaaacac
    26341 cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccgtgtttag
    26401 ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa cttaggacag
    26461 gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gtattccaga ctacgacaac
    26521 tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta tgaagaattc
    26581 ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat gcgctgttgt
    26641 taatggagag agcatgaacc cgattgcagt taccctactc accggttttt taggcgcggg
    26701 caaaactacc ctgctgcgcc atattcttaa cgaacaacac ggctacaaga ttgccgtgat
    26761 tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg attggcgacc gcgccacgca
    26821 gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc
    26881 actactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc gtctggtgat
    26941 tgaatgcacc ggcatggccg atcccggccc gattattcag acctttttct cccatgagat
    27001 tttatgccag ctctacctgc tggacggcgt gattgcgctg gtggatgcgg tacatgccga
    27061 tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg accgtattct
    27121 gctgactaaa accgacgtcg caggcgaagc agagaaactg cgtgaacgcc tggcgcgcat
    27181 caacgcccgc gcaccggtct acaccgtcac ccacggcgac atcgacctgg gcctgctgtt
    27241 caacaccaac ggttttatgc tggaagagaa cgtcgtcagc accaaaccgc gtttccactt
    27301 tatcgcggat aaacaaaacg atatttcgtc aattgtggtg gaactggatt acccggtaga
    27361 tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg gaatcggcgg ataaactgct
    27421 gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt tccagggcgt
    27481 ccagcgcctc tacagcgccg actgggacag gccgtggggc gatgaaaaac cgcatagcac
    27541 aatggtgttt atcgggattc agttgccgga ggaagagatt cgggcggcgt ttgcgggatt
    27601 gaggaagtaa gttcgggaaa gatcaacttt ttgagagttc caccgcaaca ttattacgag
    27661 tggaactctt tagatatgtc agaccaacca aagcttcttc aattgcgttt tcattttttc
    27721 atgtctcatt ttcagttcag ccaatcccca ttctgaagaa ttacaaacat cttttgttaa
    27781 ataaaaagag cttttactat taagtttctc ataaatagat ttctttttga taaaatcata
    27841 gtgttgtgtt tcagaatttt tggaaccact tatgagtgtt aaattaccta ttttatgcag
    27901 ccagtagaca tgctcttgag gttggaatct attaatccag tattcattaa ctaatgcttg
    27961 aggaagaata tgctcaattg tgattctccc cgtataggtt ttaatcacgc tctcatcttg
    28021 ttgctccata tctaagcgaa gcaaaattgc ttttattaag ttcacttgat tcggacgagg
    28081 ctcatataaa tcttcatcca gagcagctat aaaacctgaa ttatctgcat gttggtttat
    28141 ctgatttatc acagaatcaa aaggcatatc attatttatt gctaccagag cagaataaca
    28201 aaccatttct ctttggcttt taatttgttt ttttaaccac ccatgcatat ataccttctc
    28261 aaaggcggta ataaattgag aaaaatcatc taaattaaaa tcttctgtac gagccattcg
    28321 attcatgaat gccataaccg gaggaatcca ctcatctacc cctaaattac ttagcgatgc
    28381 tatttttcgt cgtattgaag ggtgttcaaa atcattctct aaaatcttag tata
//
LOCUS       DAECNO010000049        28405 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.049, whole genome shotgun sequence.
ACCESSION   DAECNO010000049 DAECNO010000000
VERSION     DAECNO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.049"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="KKO08_002165"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="KKO08_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBD6318400.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="KKO08_002166"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="KKO08_002166"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBD6318401.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="KKO08_002167"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="KKO08_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBD6318402.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="KKO08_002168"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="KKO08_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBD6318403.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="KKO08_002169"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="KKO08_002169"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBD6318404.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="KKO08_002170"
     CDS             6426..7055
                     /locus_tag="KKO08_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBD6318405.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="KKO08_002171"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="KKO08_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBD6318406.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8730..10073)
                     /gene="melA"
                     /locus_tag="KKO08_002172"
     CDS             complement(8730..10073)
                     /gene="melA"
                     /locus_tag="KKO08_002172"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBD6318407.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="KKO08_002173"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="KKO08_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBD6318408.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="KKO08_002174"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="KKO08_002174"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBD6318409.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="KKO08_002175"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="KKO08_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBD6318410.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="KKO08_002176"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="KKO08_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBD6318411.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="KKO08_002177"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="KKO08_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBD6318412.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="KKO08_002178"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="KKO08_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBD6318413.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="KKO08_002179"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="KKO08_002179"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBD6318414.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="KKO08_002180"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="KKO08_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBD6318415.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="KKO08_002181"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="KKO08_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBD6318416.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="KKO08_002182"
     CDS             complement(21746..22624)
                     /locus_tag="KKO08_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBD6318417.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="KKO08_002183"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="KKO08_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBD6318418.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25251..25586
                     /locus_tag="KKO08_002184"
     CDS             25251..25586
                     /locus_tag="KKO08_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBD6318419.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25744..26187
                     /gene="yjdN"
                     /locus_tag="KKO08_002185"
     CDS             25744..26187
                     /gene="yjdN"
                     /locus_tag="KKO08_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBD6318420.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26320..27108
                     /gene="phnC"
                     /locus_tag="KKO08_002186"
     CDS             26320..27108
                     /gene="phnC"
                     /locus_tag="KKO08_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBD6318421.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27133..28149
                     /gene="phnD"
                     /locus_tag="KKO08_002187"
     CDS             27133..28149
                     /gene="phnD"
                     /locus_tag="KKO08_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6318422.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28255..>28405
                     /locus_tag="KKO08_002188"
     CDS             28255..>28405
                     /locus_tag="KKO08_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HBD6318423.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT         7295 a         7422 c         6936 g         6752 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agcactgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctgacagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac tagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgcaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctgg atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccgcgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgt atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 cgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctgc
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgca actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac caatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc attagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgtttttta tcagaataaa taacgtcttc tgatgcgttt aaaacgtcag aaagataaaa
    25021 atatcatgtg aataaaaaaa agaacaagta gagcattaac attatcttaa ataataaata
    25081 gaggcaaaaa gattattttc tttttacgtt tcctttcaaa tgaaaacgat cgtcgtctaa
    25141 aatcagcagt acccccgaca aactcaggga ttttgtgtat aattgcggcc tttttcggca
    25201 atctgccgtt ttttggcgct tttgccctgc tgacttttga ggaaatccac atgtcattac
    25261 cacactgccc aaaatgcaac tccgaataca cttacgaaga taacggcatg tacatctgcc
    25321 cggaatgtgc ctacgaatgg aacgacgcag aacctgcaca ggaaagcgac gagctgatcg
    25381 ttaaagatgc taacggcaat ctgctggctg acggcgacag cgttaccatc attaaagatc
    25441 tgaaggtgaa aggtagctct tcgatgctga aaattggcac caaagtgaaa aacatccgcc
    25501 tggttgaagg cgaccataac atcgattgca aaatcgacgg ttttggtccg atgaaactga
    25561 aatctgagtt tgtgaaaaag aactgattgt attgtgatcg gtaagccgga taaggcgctc
    25621 gcgccgcatc cggcaacgat gccgactgcc tgatgcgacg cttgcgcgtc ttatcaggca
    25681 gcctctcatc tgtataaatt tcgaactaca cttaactggc ttctcttaac tgaggtcacc
    25741 atcatgccgt taagtcccta cctctctttt gccggtaact gttccgacgc gattgcctat
    25801 tatcaacgta cgttgggcgc ggaactgctc tataaaatca gcttcggcga aatgccgaaa
    25861 tcagcgcagg acagcgccga gaactgccct tccggaatgc aatttcccga taccgccatc
    25921 gctcatgcca acgtgcgcat tgccggaagc gacatcatga tgagcgatgc tataccgtca
    25981 ggaaaagcca gctactccgg ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga
    26041 aaacgctggt ttgataacct tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact
    26101 ttctgggcgc atggctttgg caaagtcacc gataaatttg gcgtaccgtg gatgattaat
    26161 gtcgtcaaac aacaaccaac gcaataaccc gccgggaggc ccgccctccc gcactgtcat
    26221 cgaattcccg ttaactcttc atctgttagt cacttttaat taaccaaatc gtcacaataa
    26281 tccgccacga tggagccact tttttaggga ggctgcatca tgcaaacgat tatccgtgtc
    26341 gagaagctcg ccaaaacctt caatcagcat caagcgctgc atgcggttga tctgaacatt
    26401 catcacagtg aaatggtggc tctgcttggg ccgtcgggtt ccggcaaatc caccctttta
    26461 cgtcatttaa gcggtttgat taccggcgat aaatccgccg gtagccatat cgagctactg
    26521 ggccgcactg tccagcgcga aggccgcctg gcccgcgata tccgcaaaag ccgcgccaac
    26581 accggctaca tctttcaaca attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg
    26641 ctgattggcg cgctcggcag cacgccgttc tggcgcacct gttttagctg gttcaccggc
    26701 gagcagaaac agcgcgcgtt acaggcgctg acccgcgttg gcatggtgca ttttgcccat
    26761 cagcgcgttt ccaccctctc cggcggccag cagcaacgtg tggcgattgc ccgtgcgctg
    26821 atgcagcagg cgaaagtgat tctggccgat gaacccatcg cctcgctgga cccggaatca
    26881 gcgcgcatcg tgatggacac cctgcgcgac atcaaccaga acgacggcat caccgtggtc
    26941 gtcacgctgc atcaggtgga ttacgccctg cgctactgcg aacgcatcgt cgccctgcgt
    27001 caggggcacg tcttctacga cggcagcagc caactgtttg ataacgaacg ttttgaccat
    27061 ctctaccgca gcattaaccg catcgaagag aacgcgaaag ctgcctgata tccccatcat
    27121 tgaggaaaac gaatgaacgc taagataatt gcctcgctgg ccttcaccag catgttcagc
    27181 ctcagcaccc tgttaacccc ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc
    27241 attatttcaa cggaatcaca gcaaaacctg aaaccgcaat ggacgccgtt tttacaggat
    27301 atggagaaga aactgggcgt gaaggtcaac gccttctttg ccccggacta cgcgggcatt
    27361 atccagggga tgcgcttcaa taaagtggat attgcctggt acggcaacct gtcggcgatg
    27421 gaagcggtgg atcgcgccaa tggccaggtc ttcgcccaga cggtcgcggc ggatggatcg
    27481 ccgggttact ggagcgtgtt gatcgtcaac aaagacagcc cgatcaacaa cctgaacgat
    27541 ctgctggcga agcggaaaga tctcaccttc ggcaatggcg atcctaactc cacctctggc
    27601 ttcctcgtcc ccggttacta cgtcttcgct aaaaacaata tctccgccag cgacttcaag
    27661 cgcaccgtca acgccgggca tgaaaccaac gcgctggccg tcgccaacaa gcaagtggat
    27721 gttgccacca acaacaccga aaacctcgac aagctgaaaa cctccgcgcc ggagaagctt
    27781 aaagagctga aggtgatctg gaaatcgccg ctgatcctag gcgatccgat cgtctggcgc
    27841 aagaatctgt ccgaaactac caaagacaag atctacgact tctttatgaa ctacggaaaa
    27901 acgccggaag agaaagcggt gctggaacgc ctgggctggg cgccgttccg cgcttccagc
    27961 gacctgcaac tggtgccgat tcgccagctc gcactgttta aagagataca aagcgtgaaa
    28021 ggcaataaag ggctgaatga gcaggacaag ctggcgaaaa ccaccgagat tcaggcgcag
    28081 ctggatgacc tggaccgcct gaacaacgcg ttaagcgcga tgagttcggt gagtaaagcg
    28141 gtgcagtaaa tcgtaggtcg gataagacgc cccggcgtcg catccgacaa tgtgtaggcg
    28201 ttgatgccgg atgcggtgca agcaccttat ccggcctaca gaccggagcc aaacatgcaa
    28261 accatcacca tcgccccacc caagcgtagc tggttctcgc ttctgagctg ggccgttgtt
    28321 ctcgacgtgc tggtcgtctc gtggcagggc gcggaaatgg ccccgcttac gctgattaaa
    28381 gacggcggca acatggcaac cttcg
//
LOCUS       DAECNO010000050        28338 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.050, whole genome shotgun sequence.
ACCESSION   DAECNO010000050 DAECNO010000000
VERSION     DAECNO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.050"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KKO08_002189"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="KKO08_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="KKO08_002190"
     CDS             complement(998..2020)
                     /locus_tag="KKO08_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6318424.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="KKO08_002191"
     CDS             complement(2034..3536)
                     /locus_tag="KKO08_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBD6318425.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KKO08_002192"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="KKO08_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="KKO08_002193"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="KKO08_002193"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBD6318426.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="KKO08_002194"
     CDS             complement(5851..6192)
                     /locus_tag="KKO08_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBD6318427.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KKO08_002195"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="KKO08_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBD6318428.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KKO08_002196"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="KKO08_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBD6318429.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="KKO08_002197"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="KKO08_002197"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBD6318430.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="KKO08_002198"
     CDS             12871..14214
                     /locus_tag="KKO08_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD6318431.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="KKO08_002199"
     CDS             complement(14276..14482)
                     /locus_tag="KKO08_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBD6318432.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="KKO08_002200"
     CDS             14807..15361
                     /locus_tag="KKO08_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD6318433.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="KKO08_002201"
     CDS             complement(15424..16362)
                     /locus_tag="KKO08_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318434.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KKO08_002202"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="KKO08_002202"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBD6318435.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="KKO08_002203"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="KKO08_002203"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBD6318436.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="KKO08_002204"
     CDS             complement(19573..19953)
                     /locus_tag="KKO08_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBD6318437.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="KKO08_002205"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="KKO08_002205"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD6318438.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="KKO08_002206"
     CDS             21011..21976
                     /locus_tag="KKO08_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD6318439.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="KKO08_002207"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="KKO08_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBD6318440.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KKO08_002208"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="KKO08_002208"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBD6318441.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KKO08_002209"
     CDS             complement(24511..25131)
                     /gene="fklB"
                     /locus_tag="KKO08_002209"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBD6318442.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="KKO08_002210"
     CDS             25350..25895
                     /locus_tag="KKO08_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBD6318443.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="KKO08_002211"
     CDS             complement(25879..26526)
                     /locus_tag="KKO08_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD6318444.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="KKO08_002212"
     CDS             26738..27529
                     /locus_tag="KKO08_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBD6318445.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="KKO08_002213"
     CDS             27539..>28338
                     /locus_tag="KKO08_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBD6318446.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6953 a         7542 c         6989 g         6854 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DAECNO010000051        28321 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.051, whole genome shotgun sequence.
ACCESSION   DAECNO010000051 DAECNO010000000
VERSION     DAECNO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.051"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..247
                     /locus_tag="KKO08_002214"
     CDS             <1..247
                     /locus_tag="KKO08_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBD6318447.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KKO08_002215"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="KKO08_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBD6318448.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KKO08_002216"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="KKO08_002216"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD6318449.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="KKO08_002217"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="KKO08_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBD6318450.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="KKO08_002218"
     CDS             complement(3258..3542)
                     /locus_tag="KKO08_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318451.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KKO08_002219"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="KKO08_002219"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBD6318452.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="KKO08_002220"
     CDS             complement(4443..5651)
                     /locus_tag="KKO08_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6318453.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="KKO08_002221"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="KKO08_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBD6318454.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="KKO08_002222"
     CDS             complement(6446..8131)
                     /locus_tag="KKO08_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBD6318455.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLDFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="KKO08_002223"
     CDS             8401..8778
                     /locus_tag="KKO08_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBD6318456.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KKO08_002224"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="KKO08_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBD6318457.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="KKO08_002225"
     CDS             9225..9512
                     /locus_tag="KKO08_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBD6318458.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="KKO08_002226"
                     /pseudo
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="KKO08_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="KKO08_002227"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="KKO08_002227"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBD6318459.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="KKO08_002228"
     CDS             11269..11745
                     /locus_tag="KKO08_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBD6318460.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="KKO08_002229"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="KKO08_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBD6318461.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="KKO08_002230"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="KKO08_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBD6318462.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="KKO08_002231"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="KKO08_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBD6318463.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHTHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="KKO08_002232"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="KKO08_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBD6318464.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="KKO08_002233"
     CDS             16302..16790
                     /locus_tag="KKO08_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBD6318465.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="KKO08_002234"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="KKO08_002234"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBD6318466.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KKO08_002235"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="KKO08_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBD6318467.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KKO08_002236"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="KKO08_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBD6318468.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KKO08_002237"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="KKO08_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBD6318469.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KKO08_002238"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="KKO08_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6318470.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KKO08_002239"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="KKO08_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBD6318471.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="KKO08_002240"
     CDS             complement(22242..22757)
                     /locus_tag="KKO08_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6318472.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="KKO08_002241"
     CDS             23406..25175
                     /locus_tag="KKO08_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBD6318473.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="KKO08_002242"
     CDS             25386..25709
                     /locus_tag="KKO08_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBD6318474.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="KKO08_002243"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="KKO08_002243"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBD6318475.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="KKO08_002244"
     CDS             complement(26533..27546)
                     /locus_tag="KKO08_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD6318476.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..>28321)
                     /locus_tag="KKO08_002245"
     CDS             complement(27645..>28321)
                     /locus_tag="KKO08_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBD6318477.1"
                     /translation="VPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAVRSTLK
                     EEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWLVVNQIGGKERY
                     FFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVDSWKVIVVEPKKQLTLLFGMK
                     APGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAKQIARL
                     AEQSTD"
BASE COUNT         7111 a         6821 c         7226 g         7163 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagtca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggataccta gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacacacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 g
//
LOCUS       DAECNO010000052        27601 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.052, whole genome shotgun sequence.
ACCESSION   DAECNO010000052 DAECNO010000000
VERSION     DAECNO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.052"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="KKO08_002246"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="KKO08_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBD6318478.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="KKO08_002247"
     CDS             complement(1614..1937)
                     /locus_tag="KKO08_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBD6318479.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KKO08_002248"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="KKO08_002248"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBD6318480.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KKO08_002249"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="KKO08_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBD6318481.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KKO08_002250"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="KKO08_002250"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBD6318482.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KKO08_002251"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="KKO08_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBD6318483.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="KKO08_002252"
     CDS             7537..8574
                     /locus_tag="KKO08_002252"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBD6318484.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KKO08_002253"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="KKO08_002253"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBD6318485.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="KKO08_002254"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="KKO08_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBD6318486.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KKO08_002255"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="KKO08_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBD6318487.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="KKO08_002256"
     CDS             10745..11626
                     /locus_tag="KKO08_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6318488.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="KKO08_002257"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="KKO08_002257"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBD6318489.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="KKO08_002258"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="KKO08_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBD6318490.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="KKO08_002259"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="KKO08_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="KKO08_002260"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="KKO08_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBD6318491.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="KKO08_002261"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="KKO08_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBD6318492.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="KKO08_002262"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="KKO08_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBD6318493.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="KKO08_002263"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="KKO08_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBD6318494.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="KKO08_002264"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="KKO08_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBD6318495.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="KKO08_002265"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="KKO08_002265"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBD6318496.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="KKO08_002266"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="KKO08_002266"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBD6318497.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="KKO08_002267"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="KKO08_002267"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBD6318498.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="KKO08_002268"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="KKO08_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBD6318499.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="KKO08_002269"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="KKO08_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBD6318500.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="KKO08_002270"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="KKO08_002270"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBD6318501.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="KKO08_002271"
     CDS             24893..25273
                     /locus_tag="KKO08_002271"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBD6318502.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="KKO08_002272"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="KKO08_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBD6318503.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="KKO08_002273"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="KKO08_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBD6318504.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="KKO08_002274"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="KKO08_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6813 a         6866 c         7233 g         6689 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatca
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cttggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggaata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgaggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtacgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagcaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAECNO010000053        27368 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.053, whole genome shotgun sequence.
ACCESSION   DAECNO010000053 DAECNO010000000
VERSION     DAECNO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.053"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..188)
                     /gene="torD"
                     /locus_tag="KKO08_002275"
     CDS             complement(<1..188)
                     /gene="torD"
                     /locus_tag="KKO08_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBD6318505.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
     gene            complement(185..2725)
                     /gene="torA"
                     /locus_tag="KKO08_002276"
     CDS             complement(185..2725)
                     /gene="torA"
                     /locus_tag="KKO08_002276"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBD6318506.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(2725..3897)
                     /gene="torC"
                     /locus_tag="KKO08_002277"
     CDS             complement(2725..3897)
                     /gene="torC"
                     /locus_tag="KKO08_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBD6318507.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3984..4676
                     /gene="torR"
                     /locus_tag="KKO08_002278"
     CDS             3984..4676
                     /gene="torR"
                     /locus_tag="KKO08_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBD6318508.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(4649..5677)
                     /gene="torT"
                     /locus_tag="KKO08_002279"
     CDS             complement(4649..5677)
                     /gene="torT"
                     /locus_tag="KKO08_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBD6318509.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6075..6772)
                     /locus_tag="KKO08_002280"
                     /pseudo
     CDS             complement(6075..6772)
                     /locus_tag="KKO08_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6742..7167)
                     /locus_tag="KKO08_002281"
                     /pseudo
     CDS             complement(6742..7167)
                     /locus_tag="KKO08_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7139..7930)
                     /locus_tag="KKO08_002282"
     CDS             complement(7139..7930)
                     /locus_tag="KKO08_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6318510.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNVHNTGFDITVSPGSGYGSSADAYYIAMGY"
     gene            complement(7930..8610)
                     /locus_tag="KKO08_002283"
     CDS             complement(7930..8610)
                     /locus_tag="KKO08_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBD6318511.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(8607..9806)
                     /locus_tag="KKO08_002284"
     CDS             complement(8607..9806)
                     /locus_tag="KKO08_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBD6318512.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(9807..10160)
                     /locus_tag="KKO08_002285"
     CDS             complement(9807..10160)
                     /locus_tag="KKO08_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318513.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(10160..10900)
                     /locus_tag="KKO08_002286"
     CDS             complement(10160..10900)
                     /locus_tag="KKO08_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBD6318514.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            11123..11413
                     /locus_tag="KKO08_002287"
     CDS             11123..11413
                     /locus_tag="KKO08_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBD6318515.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(11437..11781)
                     /locus_tag="KKO08_002288"
     CDS             complement(11437..11781)
                     /locus_tag="KKO08_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318516.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(11794..12858)
                     /locus_tag="KKO08_002289"
     CDS             complement(11794..12858)
                     /locus_tag="KKO08_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318517.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(12861..13166)
                     /locus_tag="KKO08_002290"
     CDS             complement(12861..13166)
                     /locus_tag="KKO08_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318518.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(13168..13770)
                     /locus_tag="KKO08_002291"
     CDS             complement(13168..13770)
                     /locus_tag="KKO08_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318519.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(13770..15785)
                     /locus_tag="KKO08_002292"
     CDS             complement(13770..15785)
                     /locus_tag="KKO08_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBD6318520.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(15963..16388)
                     /locus_tag="KKO08_002293"
     CDS             complement(15963..16388)
                     /locus_tag="KKO08_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318521.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(16392..16832)
                     /locus_tag="KKO08_002294"
     CDS             complement(16392..16832)
                     /locus_tag="KKO08_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HBD6318522.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(16843..17988)
                     /locus_tag="KKO08_002295"
     CDS             complement(16843..17988)
                     /locus_tag="KKO08_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HBD6318523.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(17992..18549)
                     /locus_tag="KKO08_002296"
     CDS             complement(17992..18549)
                     /locus_tag="KKO08_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318524.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(18524..18913)
                     /locus_tag="KKO08_002297"
     CDS             complement(18524..18913)
                     /locus_tag="KKO08_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HBD6318525.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(18900..19454)
                     /locus_tag="KKO08_002298"
     CDS             complement(18900..19454)
                     /locus_tag="KKO08_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318526.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(19451..19858)
                     /locus_tag="KKO08_002299"
     CDS             complement(19451..19858)
                     /locus_tag="KKO08_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HBD6318527.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            19857..20091
                     /locus_tag="KKO08_002300"
                     /pseudo
     CDS             19857..20091
                     /locus_tag="KKO08_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20088..21029)
                     /locus_tag="KKO08_002301"
     CDS             complement(20088..21029)
                     /locus_tag="KKO08_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBD6318528.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
     gene            complement(21041..21544)
                     /locus_tag="KKO08_002302"
     CDS             complement(21041..21544)
                     /locus_tag="KKO08_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318529.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(21549..22769)
                     /locus_tag="KKO08_002303"
     CDS             complement(21549..22769)
                     /locus_tag="KKO08_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBD6318530.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLAS
                     ADKTLVRQVVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            complement(22784..23521)
                     /locus_tag="KKO08_002304"
     CDS             complement(22784..23521)
                     /locus_tag="KKO08_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBD6318531.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(23409..24875)
                     /locus_tag="KKO08_002305"
     CDS             complement(23409..24875)
                     /locus_tag="KKO08_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBD6318532.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAFEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPEPPGGDPISNEPTADSAGGRGYRKWALRWFK"
     gene            complement(24875..26497)
                     /locus_tag="KKO08_002306"
     CDS             complement(24875..26497)
                     /locus_tag="KKO08_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HBD6318533.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNSF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            complement(26500..27072)
                     /locus_tag="KKO08_002307"
     CDS             complement(26500..27072)
                     /locus_tag="KKO08_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBD6318534.1"
                     /translation="MFRRCDLKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARAT
                     GSENLSKPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIK
                     DWPKVWRTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(27056..>27368)
                     /locus_tag="KKO08_002308"
     CDS             complement(27056..>27368)
                     /locus_tag="KKO08_002308"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD6318535.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT         6172 a         7624 c         6867 g         6705 t
ORIGIN      
        1 gtggcaatac tgttttccag atcattcacc gccgcagtga gcggcggttc gcttttcagc
       61 aacgaaaacc attcagccat ctgcgcactg gcgatttgcg tcagttgttc atcgtccagt
      121 tcacgggaga acaactgcgc cagccaggcg taaacacagg caatctgttg tgctgtcagc
      181 gtggtcatga tttcacctgc gacgcgggaa catattcgca ctgcgccacc atctccacgg
      241 ggccgttaaa cgccgtcacc tgctccactg ttccattgta cttctcaatt tccaccagcg
      301 tagtgtgcgc actggccgcc tgcgccagct gcgaagtccc gatgtcgatg gtcagcacgt
      361 tggggttacc gtatttgcac agcgcaccag gctcgccgcc tttatctgga tcgtaccatg
      421 ccccttcgtg aattcgcgcc acgccgggtg catagcggtc agaaactact gctcctgcca
      481 acacctgacc gcgagcgtta aagacgcgta ccacatcacc gttacgaata ccgcgcgcgc
      541 tggcatcctg cgggttaatg aacactggct ctttacccgc taccgtatat tgctgacgca
      601 gcgtttccga ctcacatagc tgcgagtgaa gtcggaaatc cggatgcaca gattgcagat
      661 gcaacggata cttctgcgag ccgggcccac cgtgggagcg ttcgattttc tcaaaccaca
      721 tcggatgccc ctgacaatcg tcgtagttca tatcggcgat ggttttcgag tagatctcaa
      781 tcaggccact cggcgtgccc agcggttcga gatccggatc ttcgcggaat gcctggtggc
      841 gaacaaacat ctgcggatgg tcaaactcga cgtactcttt gttattccag aagtcatcaa
      901 acgctggcag atgaacgccg cgtcctttgc cttgttgtac accttcctgc cagatgcgtt
      961 tcagccagcc catttcgtcc agcccttcgg taaaggcttc ttcacgatta aagcgacggc
     1021 acagctcgcg gaaaatatca aagtcgttgc gcgcctcgaa ctgcggcggc accacctgtt
     1081 tcatggcgat aatgccacgg ttggagtggt tgccgtactg gtcgagatcg ttacgctcaa
     1141 actgcgtggt cgcaggcagc acgatatcgg caaagcggca ggttgaggtc cactggttat
     1201 ctatggcgat aaccgtttcc agcttgcgcc agccttcaat aatgcggttg atctgctgat
     1261 ggcgatggaa tgggttagtt ccggcaaaaa tacacatttt cagcggcggc agttttaccg
     1321 atttaccgtt ccagttgatc actttccccg gttcgaggat cgcatcgata aaacgggcaa
     1381 tcggaatagt gctgctgtag cctttgtagt cactgttgtc gtgaacaggc ggaatcgacg
     1441 tagagccgga gaaaccactc agaataacgc ctttacgccc cggcgtgcct gcgccgttgt
     1501 agtgccagcc aaaaccacca cctggcaggc caatttgccc cagcatcgcc gccagaacca
     1561 caatcatcca cgcccactgt tcaccgtgct gcatacgctg cacgcaccag ccagcaataa
     1621 tttgcgttct gttcgccgcc atctgccgcg ccagcccacg aatggtttcg gcatcaatgc
     1681 cggtcagttt ttcagcccat gcggcatctt tcggctgacc gtctttctca cccagcagat
     1741 acggcaggaa ctgctcaaaa cccacacagt agttagcgag gaagtttttg tcgtacaggt
     1801 tttcactgta cagcgtatgt gccagcgcca gttgcagcgg cacgtcagtt tgcgggttaa
     1861 ccgcaatgtg cttcacatgc tcgcgcccca gatactcatg ggtggatgtg acaaccggat
     1921 cgatgctgat gacctcaact tcaccggcgg cgactttcgc ttttagctgc tcgtaatatt
     1981 cataaacatc gtgatccggg caccaccagt tcgcttgctg gtttttcagc aaatcggagc
     2041 cccacagcac aatggttttg ctgttctgca ataccagcgg ccaggaggtt tgctgttcat
     2101 acacttccat tgaaccgact acgcgcggca ggatcacctg cgcagcaccg gtagagtaat
     2161 ctccgcccgt accaacgcta ttaccatgca aggcaatagc tttcgccagc atccccgaag
     2221 cgttatggaa catccccgtc gattgccaac cactggcggt cagcaaggca ctcggcccgt
     2281 gagttttctg tacgcgttcc agttcttcat agaacatgtc gagggcttca tcccagctca
     2341 cgcgcacaaa acggttatca ccgcgctggg aggtgtcgct cagatggcgc ttacgcagcc
     2401 agtccacgcg taccatcgga taacgaatac gtgccgcgtt gtgtacgtga tccggcaatc
     2461 cggcgatcat tttcgacgga tatttatcca gttcgaacgg ttttgccgcc acaaagcgac
     2521 catccttcac catcgcgcgg atagcccccc agtgcgaccc ggtaagaatg ccctctttcg
     2581 agatgacagc ctcagtcgcc gcttgcgccg cagttgcacg gcgcggcgtt aacaatgacg
     2641 gccccagcat cccggcgacg gttaagccgc caagttgtgc cagaaaacgc cgacgtgatg
     2701 cctgaaagag atcgttattg ttcattattt ttcttccttc ttatcgccgt gagccttacc
     2761 tgcggtgtca gacgcattca tttgcagata tttcaacaaa gtgcgttctt cacgtttatc
     2821 gagactggta aagccaatca tgccgttgag cgtgccgatc caaccgttag cgtcaaagtg
     2881 ggcgatttcc ggtgcgccgt ggcactggtt acaggtgccg ttgtacaacg aatccgcata
     2941 ggcccagatc ggtttgatat cgttcaccat gtcgcctttc ttcatccacg cggtggcctg
     3001 caacttgctc cactcggtat tggtgtcggc aacggtggtt ttctccagcg ttttcacctg
     3061 ctgctgcaca tcaccacgaa tcgaggcaac aaagatgcgt ttacctggga attgggtgag
     3121 tacacgctga cgtccggcgc tttccgtcca gccggtaatt tcaatttgca gccagtcgcc
     3181 gtcacgttta aggactttca cttccgaagc aggcagcaga gaaccagagg cttctttatc
     3241 gcctttcgcc gcataaattg gcttaatatc aatagagtac agcgtgtcac cactgtcatt
     3301 agcactggcg cgcagctcat cgaactgctt acggaagccg ctactcatat ccggtaactg
     3361 gtgggcaata cctttatgac agtcgatgca ggattgatta tctttcgctg ccaccttcat
     3421 ctgacgtgcc gcttcaggat gctgcctggc atgatccatc gcatcgtagt tatggcagga
     3481 gcgacaggtt gccgagttgt tttctttcat tcgcgcccat tcacgctcgg caagttccgc
     3541 gcgtttggct tcgaattttt caggtgtatc aatggagtgg gcaataaagg tctggtaaat
     3601 gtcgttgctc gcttccagtt tgcgcttcac catgcctgga atatccggcg ggatatgaca
     3661 gtcatggcat tcagctcgca cgccggaggc gttctggaaa tgcaccgact gtttatattc
     3721 ttcatacacc ggttgcatac tgtggcaact gacacaaaat tcggttgtgc tggtgacttt
     3781 gatcccaacg tgtggcaata caatcagcgc aatgccaatc acaatcccaa ttgcgaccag
     3841 cgccagtacc gaccaacgag cactgggtcg gcgtagcgcg ttccagagtt tccgcataat
     3901 aacccctgta gaattatggt ttagtgaagc gatcttaatg agcaaatatg aacagatatg
     3961 aacagaatga gtaaaaccct ctgatgccac atcacattgt tattgttgaa gataagccgg
     4021 ttacccaggc gcgattacaa tcctacttca ctcaggaggg gtataccgtt tccgttacgg
     4081 cgagcggtgc cgggctacgg gaaattatgc agaatcagcc ggtagattta attctgctgg
     4141 atatcaactt acccgatgaa aatggcctga tgttaacccg cgccctgcga gaacgctcaa
     4201 cggtggggat tattctggtt accggacgca gcgatcggat tgaccgtatt gttgggctgg
     4261 aaatgggcgc agacgattac gtcaccaaac cgctggaact gcgcgaactg gtagtacggg
     4321 tgaaaaatct gctctggcga atcgacctcg cgcgacaagc tcaaccgcac actcaggact
     4381 actgctatcg ctttgcgggt tattgcctga atgtgtcgcg ccatacgctg gagcgggatg
     4441 gcgagccgat taaactgacc cgcgcagagt atgaaatgtt ggtggcattt gtgactaatc
     4501 cgggcgaaat tctcagccgt gagcgtctgc tgcgtatgct ttctgcgcgt cgggtggaaa
     4561 accctgacct gcgcaccgtc gatgtgttaa ttcgtcgttt acgtcataaa ctcagcgcgg
     4621 atttactggt gacgcaacat ggtgaaggtt atttcttagc cgctgatgtg tgctgataaa
     4681 aatagaccgg acgaaatccc cccggtgaca gcgagcggcg gatatgttct cggtcggcat
     4741 ttttcggcgt cagaactaaa atcggtgggc tgacattatc agacaccgat tgcccctgta
     4801 attgcctgat ggcctgctca actgccagtt ccccctgcca gaccatttga tcgctggcag
     4861 ccataatcac tcttccccgc ttcagcccgc gatacacctg atgtgaaaga taaaacgaca
     4921 ccacggtaag cggcgttttc aggttacgcc cttcacccat tgccgcctct gccgcaatgg
     4981 ccgttccggc aacgacgtcg atttctgggt ggcgttccag catctcctgc aacaggttgc
     5041 gctggatttc aatatcgtta tcaccaagcg caatatcaac aatacgcacc gggcttccgg
     5101 caatggctgc gcgaaaaccc tcgaccatct ccttactgcc cccggcgtta tcgggtccgg
     5161 gcatcagcag cacgttcagt ggtttaccgt gcgcccattg caccaaatat cgcccaggtt
     5221 gatagcccat ctgaaaccag ggtacaccaa cgcggctttt cacctgggga tcatcaagag
     5281 catttaccag ttcgatcacc ggcagatttg ctacctgctt ttgcaggtcg ggaaatgacg
     5341 tcgtgctact accgagcaaa atggcctctg cgccccactg tttacactgg tcgatttgtg
     5401 cttgctgggt agccaactgg ctgtagccgc ctgcctccag cacttttaaa tccacaccgt
     5461 agcggcgagc agcctcctgc ataccatagt tcaacgataa ccagtatgaa tctttcaggc
     5521 tgggataaag cgcgcacagt ttccatacgc gtttggcttt aagcggcata gaggcttgca
     5581 ccgtgaaatg ctgcgcatca tgccagcgca acaggttatc agccgaaaat gccgacaaca
     5641 tgaaaaggga aagaagtaaa aatagcagta cgcgcatgat agcctcatca ataataaggc
     5701 tttatgctag atgcattcca ctttgcgact caaccttttt cacctaaagg atgacaaaat
     5761 cacataaata atttaaaaat catagcatta gattacttaa tgattaaata atagactgaa
     5821 aaacgcgaca aaacacaaca tatccagtca ctatgaatca accacttaga tggtattagt
     5881 gacctgtaac agagcattag cgcaaggtga tttttgtctt cttgcgctaa ttttttgtca
     5941 acgcactggg ttaattgaat ttacaaccat gctcagtatc tcgataagcg tagggagatg
     6001 atgcagtggt gggcggactg gcttgatgag aaagcgtcct aactgggacc gaaatggacg
     6061 gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat gactttgtca
     6121 tgcagctcca ccgattttga gaacgacagc gacttccgtc ccagccgtgc caggtgctgc
     6181 ctcagattca ggttatgccg ctcaattcgc tgcgtatatc gcttgctgat tacgtgcagc
     6241 tttcccttca ggcgggattc atacagcggc cagccatccg tcatccatat caccacgtca
     6301 aagggtgaca gcaggctcat aagacgcccc agcgtcgcca tcgtgcgttc accgaagacg
     6361 tgcgcaacaa ccgtcttccg gggcctgtca tacgcgtaaa acagccagcg ctggcgcgat
     6421 ttagccccga catagcccca ctgttcgtcc atttccgcgc agacgatgac gtcactgccc
     6481 ggctgtatgc gcgaggttac cgactgcggc ctgagttttt taagtgacgt aaaatcgtgt
     6541 tgaggccaac gcccataatg cgggcagttg cccggcatcc aacgccattc atggccatat
     6601 caatgatttt ctggtgcgta ccgggttgag aagcggtgta agtgaactgc agttgccatg
     6661 ttttacggca gtgagagcag agatagcgct gatgtccggc ggtgcttttg ccgttacgca
     6721 ccaccccgtc agtagctgaa caggagggac agctgataga aacagaagcc actggagcac
     6781 ctcaaaaaca ccatcataca ctaaatcagt aagttggcag catcacccat catctgttgc
     6841 catacctaaa tcaaccgcat cctgaagcgg cgctattaca ttcgttgcct ctgctatcag
     6901 tgcagatttc ttttcatcat ttattaaggc taattgttct ggtgttggag aaggacggtc
     6961 aacaatgaca gggtgaccgt tctcaccaca ggtaatcatt tttgttacac tctgtgagtt
     7021 aatcagtgtt ttccactctg attccgagat ttccactgca tcatcaggaa tattggtgcc
     7081 gtgaaattca gtcgaataaa atccgtttgt cgatgcagaa taaaaatatg acatttcgtt
     7141 aatatcccat tgcaatgtaa tacgcatcag cagatgaacc atatcctgaa ccgggcgaca
     7201 ctgtaatatc aaatccagtg ttatggacat ttgtcgccga tacagtcggc tgccctgacg
     7261 cgccagtaac gttcaatgta actgtgacag caaggactct tgagaatgtc atcgggaacc
     7321 tcatcccgta tgttgaagat acattcgggg agccattcaa ctgaccgcac tgtaaaatta
     7381 atccagacgg aaatttttgc gcagcagccg ttgcggtatt acttgacgcg aacaggctca
     7441 tatcaggtat ctgattcgcc cctgtaccta cattcctttt agccgcttct cccaaaccaa
     7501 ggtatgtgag aatgtcagca atagtatttt tcccaataat gtcacggcca acagaagtta
     7561 aatcagtctg cgctgctgta tcatttccag tgaaatatgg gagtttattt gcacctgttg
     7621 cgagcccagc taatgccgac agcgtggcat caagcgcctg gaaatctttc ccgaaagcgg
     7681 tccccatttt ggatataaac ccgttcaggt ctccatcatc aagcacatcc agcctgcttt
     7741 tgttggcggt gtactgcgcc aacgctgccg cgataaagct ggcttgccga atagctttgt
     7801 tgacctgtgc gctggatgct ttacctgccg taaaccctga aagcagagcc ggaagtgctt
     7861 cccagtcagc ttgtgaggtg acgttagcgt tcggatcaag cgcgaaaggt ttaaagttgt
     7921 ttattgccat tagagtattg tcccccatgc tccaacatcg aacccgccga tgtattcgtt
     7981 atccatatca aacccaaaga atttagagcc ctcggatggt gcttctaccg aaggcgtttc
     8041 aacatcaccg gcccatacgc cagctgcttt aacggtgaga tagccctgtt tgatagcggc
     8101 aatcagttca agagacacat cagaaatatc agtctcgggg aatacccaaa ccgatatcgt
     8161 catgtcctgg ttgtcgacaa tttgcatcct caggcctgag ccttcagtcg cagcgtcaag
     8221 gatgggaggc agagagtcgt tccgaccgtc ccagttgttg atagcgattt tcgctttcag
     8281 aatgatgcgg tacgtctcat cgcttagcgt cgtatagcca gaatcaggat catatggccc
     8341 tttccagatg ccctggtcat acccaagccc gtcagtgtcc cagctgaaat aaactccgct
     8401 aattggctgg ctgactatgc gactgcgtcc gatccacaga ccgaggatgt cgagctgtac
     8461 accgacagca gtatcaatat cgaaggctgt tataagccct gacatagtgc tggacacatc
     8521 aatcagcggg cgggtgctca gatctatatg gtcaaaaaag agtggcttgg tagcgtggta
     8581 gttagtgatc agttcggtgt atttgctcat gaggtcaccg tgatactgat attcgcggtg
     8641 ctacaggacg cagaagcatc ataggcaata tcaatgtttg atgccgatac gctgccagac
     8701 gacttaccga tcagcaggtc ggtaatatcg taatagcggg cattcccgcc gctcacaacg
     8761 ccgaggtttg ccggggaata aatacggctc agcagaacgt cgtcgccaat tgttaggcca
     8821 tttatataat cggcaacagc ctgtttaatc tgctcgccga tttgagaggt atagccggta
     8881 aaaactttca gggtaatggc tacgaaaatt ggcacatcgg tagagcgcga aaaactgatg
     8941 acgtgtggat taccgtaagt atccggcacc gtgacagaag ttttaccgta agttgcggtt
     9001 ccctgccctt tactcccccg gattgtttgg gctatctcgg taacatcccc tccatcgacg
     9061 atggcggaaa tagagtgtgg cggcagcccg ttgctgtcgg ttgccccagt gtcgttctca
     9121 tatagtttgt gacgtgtcac gccagcaaca ttagcgatag caccgtcgac accttcaaac
     9181 ggtgtgatcg atggtagcgc gacgctttgc ccctgccgaa tgcgcagctc tgcgtcggtt
     9241 tcggctggtg aaccgacagt agccgcaact ggattggtta ccgacaccca acctcgggtc
     9301 ggggtgttga tagtggtaat agtcccggcc agcgccgcaa ccgaaccgct attcgcacat
     9361 gtggccgtca ccagcacagt accatcaacg ccgatcgcta cacttgcggg aaaattccag
     9421 ataatgccgt ttttatcccg tgcggagcca ttcgtgatag tcgtgcctgc cgtaccggtt
     9481 aacagaaggt cagcagtaga gtttgtcgct acttttcgcg tgatcccgtt aattttcaca
     9541 ttgctgctaa gcgctgcggc ctgcgctgtc gtcggtgaaa aagagttgta gatcccgata
     9601 gcggtgttgt tagcgtcatg cacggcaaga gccaccagcg cgaccatctg cccgtctttg
     9661 ctgtctggtt cgaggtaggc atcactaccg taaatctgcc tgaaatagct ggtcagtgta
     9721 tctaggattg tctggtaatc aggcgcacta atcccctggg cggttaccgt tgccgatagc
     9781 cccagcgtgt cgaggttcaa agccatttat gcctcgcttg ttacagtcgt ctggccgtag
     9841 attgtgtcaa tggaggaagt gaaggtgacg cgacggctgg tgccgtcata attggtatcg
     9901 aaggaaagaa tcgacagaac gcccggtgta tcctgtatgc gttcgcgtat agccaggatg
     9961 tagacatctg atcgctgctt cccaagcact gactgaacat acggcgtgcc ttccgtcaga
    10021 tcgagaaacc actgaccgcg ccacagctcg aaacgggttt ttacggcctg ggcgacacat
    10081 tccggactgt cgataaggaa agtatcgtca ccctggccga aagtgtaatc gccttcagta
    10141 tcttcgcgac ggtatcgcat tattgcggcc ctccggtagt tcccccgcca gtctgtactc
    10201 cgccatgttt atgcgtggcg acactgatac cagaagctgt tacgtcattc gttaccatca
    10261 ccggcccaag catcgtcgca gtaccgccgc tttcgcccat tccctgagac aagttgccat
    10321 taatcgttac gttgccgttc agcgtgatag tcggggatgt gattgtcgtt ccaccttcag
    10381 ccgtagctgt aagctggccc ggtgttttaa tggtgatgtt atgtcctgcg gcgacttcta
    10441 caaacgccgc accatcatcg gttcgcagct gcgcggcgct ggtactgata ccgctgattt
    10501 tccgtgcttg cgactgcggg ccaacgatgg cgaacgcatc agataagtca tgctggcgcg
    10561 ggtcgacggt ctcctgaacg ccgccgctct gccaccaaaa atcgatgcaa cggtcagcaa
    10621 aaatcaggag gcactcgtcg ccttctttta ccggaaaggt cagcgtgcaa ccgccgccgc
    10681 gcgggaagat gaccggcaca tccaccagcg gttttaattc ggtggatcca tcgccaacaa
    10741 taccgcgtag cgccacctct actgtgcagg taacagtctc aggatcgaac gactgaatga
    10801 tgccgggcat cgctacgcgc atctgggtag acaccgaatc ggcaatggcc tgcgcggtct
    10861 gctgctcacc gccgatctgt gattgagttg gaattggcat aaaaccccca taaaaaaacc
    10921 agccgaagct ggtttggtat caattatctc ttagtaacta agctacatca gtgtgaagta
    10981 aggcttttcc gctgcaatgg cacacattca caaggtgtat taggatatgt ttgcaacaag
    11041 taagcattga aaatcaattt cagccatgaa gccgccatag agtggacctg caccatcgca
    11101 ttattgacta caggagtaac aaatgggatt tagatttcgt aaaagaatcc ggattgcgcc
    11161 cggactcgcg atcaacatta gtaaaagcgg agtaagcact tcaattggtg gtaaaggttc
    11221 caccattaac atcgggaaaa aaggcgtaaa gatgacaaat ggccttcccg gcacggggct
    11281 gtcgcatacc actaaccttt actctcccgg gaaatcgaca gaaaaaaaac aacttactca
    11341 taagcaaaaa ataattagaa acatcctgtt cgtaattatt gtgtttataa taattaaagc
    11401 tcaatatttt tgacgcatgc ccgcctatct ggcgggctat tttacttttc ggcagtcgta
    11461 tgttgcatac tgacgcggcg aatccatgct ggcttgcagc cactgtgcgt tgagaatggc
    11521 tttgccgttt cgattgatgt actcaagacc aacccatctt ccaggctgat cggtagccat
    11581 acgccaatcc atcttgatat tgttataatc gcctttgttt ttcaggaagg tgattttttg
    11641 catttctggc tttgcgccat tgattctggc taagccatca tctgcccaat ggattttaaa
    11701 atcaccacat tgctgatccg caaaagcggg tgctgatata agcaccgcga acacggtaac
    11761 agtgcaaagt aaatgtctca cggccccacc tcactaattc gtcgtccctt gcattgcttt
    11821 cgggctataa agatcacgag cgccacgcgc aaaacacatc aaatccatgt accacgcctg
    11881 acctctggtg tcgccagtat agtcgatagc tttgacgata taaacgccat ccgtcgcaat
    11941 gctggcagcc tgtgacgtcg tgccggtcag cacacggttg ccgttctctt ctgtttcggt
    12001 gatacgcccg ggcgactgtg ctatttcgct attgccgagc gtggcgcggt acaccgaagc
    12061 ctgatcgagc tggataagac cattaatacg gatgtttggg tttatcaggc atcgcacgtt
    12121 tactccgcca cccatcgttt gttgcggcat accgatcagg ccagtattgg cattcaacac
    12181 aatggcttcg tgaatatatt tatcctccgg caccatctgg acctgaccat ccaccagttg
    12241 ccatgtcgct ttgcactgcg cagcaatatt atccatcacg ttgcggctgg atgagtaaat
    12301 cgcgcggcca cgaggaaaca cggtatcagg aaaatcgccg gtaatgccct gtgtcacgcc
    12361 gaacgcgttg aaatcctgca tcgtcgcccg gtgcagatcc gcaacggtat atccagcggc
    12421 aagcgtggtg atggtggtcg catagaggaa cgcttcgtga tcaccaatgg cctgaatcaa
    12481 cacccaggaa tccgtaatgt tgtccttccc ggtgacggtg aagcgaatat caccgtcaaa
    12541 aatcaggccg tagttctgac cgttcacctg ccctatctgg tctggtgaaa tctcccggga
    12601 cacaccaacc tggctcgcat caacatccgg cgctataccg tcatacccgg caatgatgcg
    12661 aattttggca aactcctgcc caagtatctt gttcgtggta tcggtcgaaa ggttgtaaat
    12721 tttcacgttc gccacgcgcg gccagcgtgt gtctgcccac tcgatctgga acgtgacctt
    12781 aaagtcagac agggaaacgc cctgcccgtt ctggtccaac agttgcagct caaaatggcg
    12841 catccagtta agagacattt ctactcctgt acgaaaatga ggtggctgta tgtgccgagg
    12901 ttggttttgg tgggctcgtc tggtgcgccc ttatcggtcg ccaccaccag cgcgccatca
    12961 atgccaagct gtggatattg tcgtaaaagg tttacgccgg tcagtagagg tacgccagag
    13021 agaagcgcgg caccgccgct atccatcacg tccataatcc agccagccgc atcacgccag
    13081 atgatcctga gtgtatacgt ggtattgccc agcaaaactc ggaactgctg attgtcagga
    13141 gaaagcggta tttcgttaaa ctgcatatca tcccccgaat gctgatgtaa cgcttccgcc
    13201 cagctggctc agcaaggatt cgtttggcgg tgtagtggat ttcatcccgg aattctgcac
    13261 cgccgatgtg ctgacgccat cctgcatatc tgatttatct gcaacgctaa cgctctgcgt
    13321 ctgcgacatg atcacttcac gcagggtaag cgtgcagttc agcacgttct cgctggtttt
    13381 atccgttgtc acctcgatgg ctcgcaccag catattgctg taaacccgct ttccggtcac
    13441 tacatcgaac ggcaccctag aggactggag atccagtagt tgctgatagg tctctttcgg
    13501 gctaagtccg gcgctgaggc cgattgacga tgtatcaatg aagtccagca acgaaccgcc
    13561 acctgcgaag ccgcattcca tcgtgacttc gctggggcgc ttatacgcat gatcggcgat
    13621 gaaacccgac gcgctattcg ttgttggctt ctccaccggg tgctcagtaa tttcgagcgc
    13681 atcagaatgc ttttcagaaa caaccacgct ggggattaat attccaattc gccgggattg
    13741 ctggcgaaaa attgctgata aaatatccat tatctcggtc ctgcggggag ttgctgggtt
    13801 aactgtgaat tcacgccctt ttgacggtca acagtcaaac gggcagcctc gcgcggatcg
    13861 aaaacgccgt ggatgttaat attcgtttcc tgctgaatca ccggggcgct ggtgggcata
    13921 ttgctcatta ctttcggaat gtagttgcgc gtttcctgcg gcattagccc cattccataa
    13981 cgcttaacat tcccgatccc ccagttatat gatgccagtg ctttgctaag gtctccgccg
    14041 ttctgccgca acagctggct caggtactta gcggctgcct gagctgactt ttccgggtcg
    14101 aatacatcat tcccacgcag gcccatgtcg cgcgccgtgc catccataaa ctgaaacaga
    14161 cctttagcgc cagcgcctga aacggcgaac tgattaccgc ccgattccgt tatggccacg
    14221 ctgcgcaata acccctccgg aagccggtag agctgttcca ggttggtaaa catcggctgc
    14281 atccatccca gcagttcaga gcctgctttg gttggctgtg gccgcttaac tgactggccg
    14341 agctgctcag gctcatcatc accgaaccag ccgcgcaccg ttcggcccac gctgcgagga
    14401 tcgaatcccc agtgctcttt aatccagtcg gcggttccgt tggcgctgtc tgttaccatc
    14461 ggcatcgctg acggattttc gctgccctga ttaagcatct gtttgccgat gctggcggca
    14521 tcagcccagc ggccatcttt aatggcgttg agcaggtcgg cgatcatgtt cagcatcttg
    14581 ccgaactcac ccatttgcga aatgaagttg ctaaaatccc actttaggga ccatgacttg
    14641 gggtcaatac cgagcaactt agccagcgct tttgtcaggt ccgtaacagt ctttttcagg
    14701 tcaccaatca ttttgacggc ctgatcaatt tcagtcttcc atttccccca gtcaatcagg
    14761 cttttgccac cttctttcca cgtctggtaa tcatccaaga gcagaccaag tgcaataata
    14821 agcgtcgtta tcatgcccaa gggtgatgac atgaaagcgg tatttagcag ccgccatgca
    14881 accaacagcc cactgaacaa tgcgataagc tgctgcgtgg ctgggttcag ctttttgaac
    14941 caggcgatca caccctcaac agcttgccca gttcgccata acacgcgggt aatcgcgtca
    15001 cctgcccaga gaattccttt gatgatcttc gtgatgaccg cttcaatctt cggccagttg
    15061 tcgagtatct gcctgcggaa gttatcaata ctgccagcaa ggccaccagc taggttagag
    15121 ccaatcttgt ctttcgcctg tccaagcgtc atcgtcagat tacgcatgga ggtcatgaaa
    15181 atattggact gtttagccgc tgactcagca ttaaaaccaa tacgctttgc cgtcaacgcg
    15241 tactcagaac tgagctgccc catccctctg cgcatcgcca tcagcgtgtt ttcatcgatg
    15301 ccgagcatct gcgcgtattg cttcgcgcgg taatacggca tgttgttgag cttttggcca
    15361 acgccagtaa agatggcagc agtatcacgc atctttccgc tggcatcgcg ggtctggaca
    15421 ccaagacggt tcaggaaccc ttccgccccc ggattgctac gcatgaagcc agccaaccct
    15481 tcgagggagg acatagccga ctcggcgctg gcaccggttt gcgatgcggc gtatcccagc
    15541 gctttgatgc cctggacact ggcccccgtc cgctgggatg cccagtaaat tttatccaga
    15601 ccgttcgcga tctgagtggt aaatccgaca atgctcagcg ctgcgccttt caccaccgcg
    15661 ccgaccttca gaacgttcgc ggtaacgcct ttcagcacgg cttcaaactt attagcgcca
    15721 gcctgatcga tatcgaatcc cagcgaaaca aggaaatctt taatcgtatc tgcgttaccg
    15781 ctcattggcc gctctccatt tatctacccg ggcgtcgtta tcctcgcgca tgtcgaggta
    15841 gtcattgaga agcgcgatgc ggcaaagatc taccgcaccg ctgttaaggt ctttctggtc
    15901 aatatggaag gcaagcgccg ggcgaagaat aaaatcttca ccgcccggca ggctgttgaa
    15961 ggttattccg ctagcggggt gggcgtctcg ctggtaggga gtccttgcaa aaaatttccc
    16021 agcgagtcgg cgaccacccg cgccaccagt tgcagcatgg taagcaggtc gatatcgtca
    16081 aacgccattt cgccatgctg gcagaccggc acccagcctt tcatgtgctc gcgtgaaaca
    16141 acggaaagac aggggaacag gatagcgtcc acgtcgccat cactcagatc ggacacagca
    16201 ttggcaatct ttggcaggat agtagccatc gcgccttcgg tgtctttgct gctgatcttc
    16261 tcctgaacgc tccggaagtc cgaaaccatc ccggccagaa ccggcaacag cttgcgggac
    16321 accttcagct gttcgaaaac gctgagcttt gcggtgcgat atttcacgcc tttaatttcg
    16381 aattccatgc gttaaaactc cccgagaagc tggtcaatct tgccgcagtc gaatacccag
    16441 gcgacggttc cgccctcttt agcgttattg aaatcaggct gtttctggaa tgcacacgaa
    16501 cgcgcagtag aaatatcacc cgatgccgtg ttgcgaatga cgatcacgtt attgccccag
    16561 gtggcagagg actggctttg cgcgttatac gccagagaca gctttttatt caccggggag
    16621 gttttgagta gcgtcaccgt aatggtgcct gacttatcgg cgtgcaggct gtgcatcacc
    16681 tcgccatcgg caccgatagt catggtgttt ttgttgccgc ccatggtctg ggtgatacct
    16741 tcctcagagt tcgcagaacc ctgaccaaga tcgataacgc cggtcggccc ggtgagcgac
    16801 gcggttacat cgagaaaaga ataagttgcc atttatcgct ccttagcgaa ccacgttgat
    16861 ctgcacatcg gcataatgaa ctgcgccagc cagcttacag gccacctgga ttaacggtgc
    16921 tttgcgcgct tcacggtcgg cctgcgcctg ttcggacagc ggttgcgcat acacgtaata
    16981 gcctttggtc agcgtatcgc cggaattcag ctgtccgata gggccaccat tccacacgcc
    17041 agcagctacc agaccgttcg tgacggactg atccatggac tgttcaacgt tggaaagcag
    17101 acgggtcaca ccggcatcag tctgcggaat tttggtggtg ctggtgtaaa gcaggttata
    17161 gaggttggtc tgaacgtagt tctgcaacca gtcgagcccg tggcgctcgt cgaagaagtc
    17221 accgttcgcc atgacaccct gttgcaggat cgccgtgtcg ttggcgtagt acacgaatac
    17281 attgcagttt tttgcatcaa gtgccgatgc ctggctgact gtcagtgttt catacccgac
    17341 acccggctcc tgcttaaact tgagcgtaat cgcggtatta ctgccattga aattaaccgt
    17401 gaatgcccgg ccaaatgcag ataacgcagc gtatttatta cccgatgaat attgaataaa
    17461 actgcgtgaa tatccggcgg ttttcagttt tgatgccaaa tcatcgctgg atgcagtctg
    17521 caggcatttc tcatcgcttg tcgtaatcgc cagaatacgg cttacagaag aggattcgat
    17581 cgccgcagcc actttcagcc agtctgcatc cggaatatct tcatcgtctg caatccccag
    17641 cccataccat gaagtataat cgagcatggc attcacagcc tgctccagcg tctcaggcgt
    17701 ggcctgttcg ctgtctccct tcgttttcac ccaacgacca acaaaaacct cctgaggttt
    17761 cggtgattgt gagaaaaaca cctgcgcagc cttatattct ggtgattcca cgccaaaatc
    17821 ttttccaata tcttccgcgg cagaataacg gcgaatgcgc tcacttaccg gaatgattgt
    17881 ggacgggccg agaatgagta atgcaccaaa atttcgccct gatgctgcac gcggcgacat
    17941 gatcacatca acattaacaa cgtttgatac aggcaagccc tgtgccatag cttaatctcc
    18001 gaaaaagatg actggtgctt ccaccagcga tttaataccg tactcgcgca caaccttccg
    18061 gcgcagacgc accgtcatat cgtagcggcg gacccattgc tgattaataa gttcagggaa
    18121 gagacctgtg taatcgccaa gagacagccc cagcgcattc agtgctgcgt tgttctgcgg
    18181 tacagatata ccgtcacgaa accgggacgc atacaccatc cccgccggac cataaaacga
    18241 agccatacac tcaatcgttt catgccgcca gagctgagag ccatcatcgg tctgtctggt
    18301 gaatgccgga ctgtcatcac ctgaccatcc gataacccca aacgcacacc agttcgtttc
    18361 aaccggtagc agtggcggct gctctttctg ccagcgcggg cgaaccatcc cggcagacag
    18421 accggaaacg ttacgcatcc actggcttaa cagcctgtcg agcgcttcgt cataatccgg
    18481 atcgccactg gttggtatta accatccgcg ctctgtactg gtgttattgc tcaaccggag
    18541 ttcccccatc aaacggcatc aactcacaat gcgcctgaac gaatccggcc ccataagctg
    18601 tatacgggtc gacgaaggtc acacgataat cacggccctg atacgtcacg atatcggcat
    18661 cacggccagt ctgtccctgc gtcagtcgct cagtcgtcac aatcagaatt gcaccgctga
    18721 ttacctgccc tgcctgcata cggcggtttt ccagagagcg atcaacagtt acgactccgg
    18781 caaactgctt tttaacttca ctgtcgctgc cgatcccgtc ctcatccacc gtttgcactc
    18841 ggcgtgttac ccacaaattg aagtcgcaaa aatcggggtc aaaaagcaca tctgttacat
    18901 caagagtcgg catctttatc cctcacaaca tgggtaatag ctctgcgata ttgcccggtg
    18961 tcaattaatg gtttcgccag atcggttccg ggagattcgc cagcaacacg ccgagcaagt
    19021 tccagtgttg cccccttgcg cccccgacga gcccgggctt caacggtgct atcagcaagc
    19081 ggtgtaaagc cggtaatggt catgtaacgc ctgacgccat tcgcggccag cgttccggcg
    19141 cggttaagcg ctctttccgc acccgccgca tttccatcaa gcgcagcctg cgccgctgct
    19201 ttgagctgcg gcaccgtctg ttcctctacg gatttaacgc cggggatcag gtgcgggcgt
    19261 gggggtatgt tttgcgctgg tgagccgtat tcgttgacgt aaccgatccc ggcattacca
    19321 aacggaacat cctcacgctc gctgtcttct tccgggatgc ccaccagcac atcctttttg
    19381 gttagcgacc ggagcgcatc cagaatggcc tgagcgttat caaccctcgt tgttacacca
    19441 cttttgaaac tcatagctgg cgaccgcccg caccgaacat cgtgatcagc tgataaaatt
    19501 cagcgccata tcgggtgtta ttccagaagc ctgcgtcagg gtttagcgtc gcgctggtgt
    19561 catagctgac gcttaccttg tcaacggact tggaggactg aacaccattg gttgaaccgc
    19621 ccgggccgcc aaccagcatt gcccgactat ctgccgccca tagcgtcatg tagtgagcca
    19681 cgaacaaccc ggcaaagtac ggaaacaaat ctttgccggt gacgttttcg ctcagcagtt
    19741 catcggccag attcagacga aactggattt gcgcttcggg atatttggca gggtcagcaa
    19801 actgcgggaa gtcgcggcga aaatcactta ccgctggcag gctttgattc tttggcattt
    19861 tttacctcgt tacgcgcgtc tgtggctttg ccaacggata cttccgcgtg cgcacgagtg
    19921 aaccagtgcg tggcaacgtc ttcctccact gcatgacggc ctttaacaaa ctcgcgccgc
    19981 gaaccgtcgg gaagcgtgag cacaaacggg gtatgtacgt gtattactgc attatttttt
    20041 gccatcgggt catccttaat ggccccgcca ggggggccat gtggctgtta aatgccatca
    20101 acgtacgaaa tggtttcttt gtacactggc tcaaccgcac ccagcttgcc gtagtaggtc
    20161 gcaatctgat acagaccgcg atactggata ggaacgctct gcaacggcac cagcggatag
    20221 cggacgtatt ttttatcgtt ggtgtacgca accatgcgat ccttattccc cacaccacgg
    20281 cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg atagctgatg
    20341 gtgttggtct gaaggtacgt caacagggac tggttacccg cagatgaaac gatgatgctg
    20401 gacaacagag caaactgctc tggcgggatc agcaaatcac gcggaaccac agagtaaccg
    20461 gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc gtccggtgtt
    20521 gagattgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt aacaaggcct
    20581 ttcaggttta atgcggaatc gccaacatac acctgctcgt cgttgtccat ctgccatttg
    20641 agctgcatcc cgtcgtactt ctgggtgtca atcgggcgac ctacctgctg agctgctgcc
    20701 agctctacaa cggtccagcc cagttccatg ccccagagat tcagcgggtt accggatttg
    20761 ccgatatcca cgtttacgcc agcaatagcg gttgagtctt tgcctaccca gtttttgcca
    20821 ttcggatttg caccagtacc cgcagcggcg aagctggtat tcgtccagct ggaaatgtca
    20881 tctgcgatag agacatcttc acgcaactga atatcgcggg tccaggtgta ccccgccagt
    20941 ggcaggttca gagtctggtc gagtcgctcc agctctccga tgagaaaggc accagagctg
    21001 tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc caggaaattt
    21061 ctgcattgcc gttagcatca ccggcacctg tgaattcagc gttggtcagc accacatttt
    21121 tgccactgac tgacgtggac atgaatccac ccagcggcac tttgatggat tcatcagtgg
    21181 agacgacaac gtataccggg tcgccttttt tgatggtgct ggcatcaaaa tcagaaccga
    21241 gattaacggt cacgtagcca cgcttcatgg cgtcgcccgg gaagttcttg cctgttccca
    21301 cctggcgaac catgtccggc tgcgaagtgg tcggataagg gcgcacgtag atccccttca
    21361 ccttgtctgc ggtatcgcca tctgccagcg gcacgaaaaa accgtcagca tcgtatttac
    21421 cagccagccc ataggcagca aaggcgttat cggatttaag gaccaccggt tcgacggtta
    21481 agtcctgcgg gcgagagata gccccggcaa tgccaacagg catccggtac agatatgcag
    21541 tcattggatt atcctttgcg gttagaccag aattcagcgt tttgtttgtt cagggaagcg
    21601 atgctggtca tgcccatgtt tggacgttgt gcatcgcccg tggtgctgcg ggtgtttcgc
    21661 cctttggcaa tctcagacac agcgttaaac gccatatcga ccgattgctt aggcaatttg
    21721 cggatatccg catcaccgac aacctggcga accagcgttt tgtcagcaga agccagaacc
    21781 tcgcgtttga acacggtcgg tttcatctta cggctcagat cgatacccgg aacgataact
    21841 tcggcacgcc aggctgagtc accagtaatc gtggtttcct cttcatcgtc ctcgccgtca
    21901 ccggtcggat tatcgtcagg tttattatcg ttatcgcccg tggcatttcc ttccagctta
    21961 gccagcaggg ctttcagtaa tgttttgagg tcatcatcac tgtcgccggt tggacctccg
    22021 cccatctctg gtactttgtc cggtagcggt tgctgcgggg acaggttgat gttgagatta
    22081 acgccctgcg gcaaatcccc ctcatctcct gtaaccgatg cgggagccga ctccaccagt
    22141 tcgttcatgg tgtcagcgtc acccgttttg atggccgtgc gcatgcgggt ccaccagctt
    22201 ttcttttgat ttgccattgt gtctctgtct ccaattgcac aacgatttcc ggctctgcct
    22261 ttggggacaa gagccacatg gtttccggta atatcgacct gctcggcttt acctggctcg
    22321 gtctgctcgt actccgcgtc atagccgcac gacacttcac gcaggccatc ttcgataagc
    22381 tgaatggcgt tttcgtcttt gacgataagg tcagccagca tcaaatcaga gtggtcaccc
    22441 gtcccgcgcc gcacattctg aagatgcccg acagcaagct ctttccagtt ctcgggattt
    22501 accagccgca cattcccgtt ttcatcttca ggatgcagga tcgtgatgct catcccttcg
    22561 aatgaggcga gcgtggccgg atggaatacc tgctcaggag agcgcgttac gactatctca
    22621 ccgagcttgt cgggtttgag gtttggcaga tcggcagcgc cgtagagctg tttacccgtt
    22681 cgacctatcg gcacgtcttt gcacagcagc gagccgtcag ccagctgata gcgggtttcc
    22741 cccagccggg tattgaaaaa atatttcatg gtttacctgc gattcaggcg agataagaat
    22801 gagggttggg aaaaacgatt tctttataac agcgacaatt cgggagctcg ccagcgtgac
    22861 cggtcatgcc atcaagcgtt ggaggtttgc cccattcgac aaacttacct tccatctccc
    22921 gatgagaatg ccggacgtcg ccatcttcgg ctgtacgcca gatataacca ttcgagccga
    22981 ttgacagcgc acgcgcctga tcgagcgcgc cggttgcacg tccaagctcg gtacgggcga
    23041 taaggttcgc tcgtgagcgt gacacgtcac cggacgctgc tatctctttc gcgaatggtt
    23101 cagcgcggcc accagccaca acggcctcga tggccttgtt ctgaatgtca tacacccgat
    23161 cggcggcctc aagaggcagt gacttgatat acttaatttg ctcggcgacg atggatttca
    23221 tcacctggcc taccggggcg cggtcgacca tgttgcgcag ctctgcgctg atgttccggc
    23281 tgtgctgacg ccactgcttt tcattctggc gtgcaatgtc ggcggtaaag ttctcagcaa
    23341 ccttcgtcgc ccagggggtg atgatttcgc tgtagcgctc cagcgcatcc attatttcgg
    23401 taacgctatc atttgaacca tcgtagcgcc catttacgat atccccgacc gcccgcgcta
    23461 tctgccgtag gctcgttcga tatcggatct ccgcctggcg gctctggcgg tttgtcgcca
    23521 agttcgccga tgcctggcgg cgcttcgtct tcggcattct cgatatcctc gtcggtaatg
    23581 gatgccccga tgccggtgac gtcagagttt tcgcgcaggt cggtcatcgc cgccttacgc
    23641 gtcatcaatc cgtcgcccag cgcggtacta atcgcgttgg tggtgtttac ggccaccgtt
    23701 gatcggtcaa cgtctgacat ttgccatagc gggttaaact caaacgtgaa atcgtccggc
    23761 agcggctttc cgagttccga gcggtgcata atgtccagta tccggcgcat cggcagccgt
    23821 aagcggcgct cctgcaatga gctcacccgg tcgtaatagt tggcgaggtc tgcatcacca
    23881 gtagagaagc ctttcgggga ttgaccgaac aaccgcacca gcgggatacc aacggcaccg
    23941 ctaatctgct cagcaaactg cgaaagaatg tcatccagac cactaaagct gtactggtgg
    24001 gtttcgaact tatcccgcga gtccatgagc gtcatacctt cattgctctg gaactggcgg
    24061 atcaggtcga tgttcttcag caacgcctcg aacgccgggc ctccaagcgc gataagctcg
    24121 cgcaacttct ccacgctata ggtacgcaga tgtgctttgt agaccagctg cgccgcgccg
    24181 acagtggcgc tgtcgaacgc agtaagccga tcccagatac gctctacaac cgacattccc
    24241 cattcgtttt cggtcatctt ctgctggaat ggcagcgtca ccccgtcgaa gcgaatcagg
    24301 cggctgtgat ggatgcgcca ggccgggatg cccgttgcag tggtcaccac gtcgtaaaac
    24361 tcaggtttgc cgagatctgg ccccatctct ttaatgcggc gggtcaggac cgggttaatc
    24421 atccagcggt cgagcgggag aatgccctta aacttgcctt ctccaatggt ttcgagccgc
    24481 agcggggtca ttggtgcctg cccctcgatc atgatgaagc cgaccgcgcc gccgtagagg
    24541 cgagaccatt tcagcacgtc gttcagcgca tcccagattt gcaactcatc cagttgtgat
    24601 tcgagaatgc cacgatcttt tgcatcaatt tccgaagtga tgcgaatgcc tttgcgggtc
    24661 atatcatccg ggatagcatc gaccgcttcg ccgataatcc aggacgaacg ataggaccat
    24721 tccaccagca tgcggttacg actggtgaaa ttagcccggt aggtggatgc tgagtgctgg
    24781 ttaggtgtct gcatccccac gcgggcaaca aagttctcat agccatcagc ggtggcctgt
    24841 gccgttcgcc gcgtggcttg tttgtttcgt gccatcaggc ctgtctccct agcagctccc
    24901 agatgttcag ggctgaattc attggcgcgt agctgatcat caccgagtcg gcaaggtttg
    24961 gcgaccgggt tccatcaggc tgtttatcaa taacgatttt tcccacacca ttaatggaat
    25021 aggtcggctg cgaaagctcg atgatgagtt tatctttgag tgccatgcta ctgctgattg
    25081 agatgatttc gtccgggttg taatccatac cttcaaccac ggcgcgccag gtattctgaa
    25141 aaagtttacg taaccgccac cagctctggg ctttggcgtt agcgaagaag tccttgttca
    25201 ggcgggcggc ctgtccgttg tcaccgcgca ccgcttcgtc gtccggatca aataccgcgc
    25261 cactacctcg aaacggtgtg gcgagtattg acggtcggcg cgcagcgtta cgcagttcgt
    25321 tgatagcgcg tgcatcgccg cgaacgccag cgcccaaccc gtcctcgtca aagcgaaact
    25381 cttcgaggtt gtcctgttcg caaaagccga aaaccttctc gacggactga taaatgtcgc
    25441 tgcccacacc ggaccattcc cgcacattct ccaggaggaa gccatgacgg gtggaaaagg
    25501 aatttttgtc cctgccttcg tcggcgacgt ccatcgcgcc cagtcgcttg cctgttggct
    25561 ggatgcccag cttgatatgc gcgtcgacag cagcctgtac ccattcggat ggaatcagga
    25621 cgccttccgc tgatgcactg tagttcaggt caagttcctg tgccaccacc accggattgt
    25681 cgattttctc gcattccctg cgataccact cttcatcctt gcgaggatca tcccgccagt
    25741 ggaatgtgaa taccggtatc ttcccgccat gacgcttctg agcgaacggg ttcgccatgc
    25801 cgttaactga actcaggtca atacggcaac gcgtcgtttg tgacaacgcc gcatcaatca
    25861 gcagaggacg ctgaaggaat gcagactcat caaccagata aagcgtggta cggtcaccac
    25921 ggccaatatt atcgccagcc tcacctttga taacggcacc agtttcagga aactcaacgc
    25981 gcatgtatgg tgcgtgcttc ttttcgtccc acgaacctcg aaactctaca ggtagtgttt
    26041 ccacgaactt gcgcgccttc cagaacaatg ctttcgggtc accggtgctg tcgacgtatt
    26101 cctctttacg ggagccgaaa ccgataacca tttctttgtt gaagagacaa agcgagcatg
    26161 ccagcccgat cgcggtccaa ctgagcccca tttcacggga tttttcggta ataccattct
    26221 cccgattacc ccagcgttcc ataatccagt ggatccactc ctcctgctta gggaagagta
    26281 aaaacggaat ggtcaccggc aggccataat caatattacg cgggtccgtt gtcatgcccc
    26341 agtcgatgat gaactgagcc ggattggttc ggtaaaactg ttttagtgca ggcaatattt
    26401 caggattctg gcgaatgcgc tgtaggcgtt ccatccgcca ttcaaaaacc atctggtaat
    26461 caggatgttt aaaatcgaag gggaatggta acggcatact tagcccatca tttttctata
    26521 cgcctctgca gcctgctccg gcgttaagtt ggtaatttct gttctgactg gtcctccgtc
    26581 agcaccagtc acttcatttt tgacattgtc tttaaacgcc tgaacagaaa catgacgccc
    26641 gagcaactca aggtttttaa ccttatcagg ccatttgatt ttcttcagga gagcagcact
    26701 atctgctgat gccatctcga cgacatccat tcctgatagc gttgtacgcc acaccttagg
    26761 ccagtcttta atctgcttca gttcgccatt ggcaagcaga atgtcaagca cgtccatctg
    26821 atcgatttca gtcagccgtc gcaatacata ggcggcgtct atacctacct cttcattgcg
    26881 ttcagccttt agctcagcaa tgcgatctgc tatgtcaggt tttgacaggt tctcgctacc
    26941 agtggcgcga gcagttttct cgctgtaacc cgccctgata gctgcctgag tggcgtttaa
    27001 atcttttaaa tattccctgc agaacagttc ctgcttaggt gtgagctgtt tcaaatcaca
    27061 cctccgaaac attgctccaa tttctatgat taacgatgtc agcaatcatg gtagtggata
    27121 cgccatatct ctttgctaaa gacctaagcc cattacctac actcccgcgc acatactcgc
    27181 ggcgaatgca gatgacatcg cactctttta atttcgccat tccgttcttt tcacctgaac
    27241 ggtcagggta tgagtttctg cctcgccttg acctgtcacg attattgtca gcgacagtac
    27301 ctataatcag atggtcagga ttcacgcatg atggattatc gcatttatgc ctaacaacaa
    27361 ggtccttg
//
LOCUS       DAECNO010000054        27066 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.054, whole genome shotgun sequence.
ACCESSION   DAECNO010000054 DAECNO010000000
VERSION     DAECNO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.054"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..470
                     /locus_tag="KKO08_002309"
     CDS             <1..470
                     /locus_tag="KKO08_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HBD6318536.1"
                     /translation="GDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(467..607)
                     /locus_tag="KKO08_002310"
     CDS             complement(467..607)
                     /locus_tag="KKO08_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBD6318537.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            849..1142
                     /locus_tag="KKO08_002311"
     CDS             849..1142
                     /locus_tag="KKO08_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBD6318538.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1214..1873
                     /locus_tag="KKO08_002312"
     CDS             1214..1873
                     /locus_tag="KKO08_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBD6318539.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1965..2411
                     /locus_tag="KKO08_002313"
     CDS             1965..2411
                     /locus_tag="KKO08_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBD6318540.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2618..3518)
                     /gene="hlyE"
                     /locus_tag="KKO08_002314"
                     /pseudo
     CDS             complement(2618..3518)
                     /gene="hlyE"
                     /locus_tag="KKO08_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3891..4310
                     /locus_tag="KKO08_002315"
     CDS             3891..4310
                     /locus_tag="KKO08_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBD6318541.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            4310..5578
                     /gene="umuC"
                     /locus_tag="KKO08_002316"
     CDS             4310..5578
                     /gene="umuC"
                     /locus_tag="KKO08_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBD6318542.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5624..6154)
                     /gene="dsbB"
                     /locus_tag="KKO08_002317"
     CDS             complement(5624..6154)
                     /gene="dsbB"
                     /locus_tag="KKO08_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBD6318543.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(6300..7841)
                     /gene="nhaB"
                     /locus_tag="KKO08_002318"
     CDS             complement(6300..7841)
                     /gene="nhaB"
                     /locus_tag="KKO08_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBD6318544.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8062..8781
                     /gene="fadR"
                     /locus_tag="KKO08_002319"
     CDS             8062..8781
                     /gene="fadR"
                     /locus_tag="KKO08_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBD6318545.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8833..10365)
                     /locus_tag="KKO08_002320"
     CDS             complement(8833..10365)
                     /locus_tag="KKO08_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBD6318546.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10695..11993
                     /gene="dadA"
                     /locus_tag="KKO08_002321"
     CDS             10695..11993
                     /gene="dadA"
                     /locus_tag="KKO08_002321"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBD6318547.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            12003..13073
                     /gene="dadX"
                     /locus_tag="KKO08_002322"
     CDS             12003..13073
                     /gene="dadX"
                     /locus_tag="KKO08_002322"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBD6318548.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(13241..14655)
                     /locus_tag="KKO08_002323"
                     /pseudo
     CDS             complement(13241..14655)
                     /locus_tag="KKO08_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15266..17002)
                     /locus_tag="KKO08_002324"
     CDS             complement(15266..17002)
                     /locus_tag="KKO08_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBD6318549.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17097..18011)
                     /gene="ldcA"
                     /locus_tag="KKO08_002325"
     CDS             complement(17097..18011)
                     /gene="ldcA"
                     /locus_tag="KKO08_002325"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBD6318550.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            18111..18722
                     /gene="emtA"
                     /locus_tag="KKO08_002326"
     CDS             18111..18722
                     /gene="emtA"
                     /locus_tag="KKO08_002326"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBD6318551.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18724..19458)
                     /locus_tag="KKO08_002327"
     CDS             complement(18724..19458)
                     /locus_tag="KKO08_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBD6318552.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19659..19913
                     /locus_tag="KKO08_002328"
     CDS             19659..19913
                     /locus_tag="KKO08_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBD6318553.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19963..21423)
                     /locus_tag="KKO08_002329"
     CDS             complement(19963..21423)
                     /locus_tag="KKO08_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBD6318554.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(21360..21917)
                     /locus_tag="KKO08_002330"
     CDS             complement(21360..21917)
                     /locus_tag="KKO08_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HBD6318555.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21943..22797)
                     /locus_tag="KKO08_002331"
     CDS             complement(21943..22797)
                     /locus_tag="KKO08_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBD6318556.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22797..23606)
                     /locus_tag="KKO08_002332"
     CDS             complement(22797..23606)
                     /locus_tag="KKO08_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBD6318557.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23616..24374)
                     /locus_tag="KKO08_002333"
     CDS             complement(23616..24374)
                     /locus_tag="KKO08_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6318558.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(24371..25351)
                     /locus_tag="KKO08_002334"
     CDS             complement(24371..25351)
                     /locus_tag="KKO08_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBD6318559.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(25351..26373)
                     /locus_tag="KKO08_002335"
     CDS             complement(25351..26373)
                     /locus_tag="KKO08_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBD6318560.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6804 a         6587 c         6999 g         6676 t
ORIGIN      
        1 caggagacat cacgtactta cgtacagatg aaggctggct gtatctggca gtggtcattg
       61 acctatggtc acgtgccgtt attggctggt caatgtcgcc acgcatgacg gcgcaactgg
      121 cctgcgatgc cctgcagatg gcgctgtggc ggcgtaagag gccccggaac gttatcgttc
      181 acacggaccg tggaggccag tactgttcag cagattatca ggcgcaactg aagcggcata
      241 atctgcgtgg aagtatgagc gcaaaaggtt gctgctacga taatgcctgc gtggaaagct
      301 tctttcattc gctgaaagtg gaatgtatcc atggagaaca ctttatcagc cgggaaataa
      361 tgcgggcaac ggtgtttaat tatatcgaat gtgattacaa tcggtggcgg cggcacagtt
      421 ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag gcctgtgtcc
      481 atattacgtg ggtaggatca ctatggcctt tgctgaactc aacattgaca ttcttacccg
      541 cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac agccttactg atgctcttga
      601 ttttcattgg tagtctcctg ttcagaatga gtacgatgaa ctgttataat ataacaatcc
      661 cttacgggag aattccagtg gcttttctgc gttcggtaaa atcccggcgc ggcatttttt
      721 gccgggggga ttacttataa aggggcatgg actcaacaaa ccgcagaaat ctacagctgg
      781 ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga
      841 gtaaaagtat gttttgtgtg atttatcgaa gcagcaagcg tgaccagacc tatttatatg
      901 tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc
      961 ctcagttagc gatgattctg ccgttggatg ggcggaagaa actggtcaat gctgatattg
     1021 aaaaggttaa actggcatta accgagcaag gttactattt gcagttaccg ccaccacccg
     1081 aagatttgct gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat
     1141 aaccgatatc cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg
     1201 tgaggggaac gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt
     1261 gagtaaagta gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc
     1321 agtgcccgaa gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca
     1381 gccattggcg atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtgtt
     1441 gattggcgcg acgctgcgtc aggcaacgga agagcatgtc cgcaaagcca ttgccggtta
     1501 cggcgtggcg cttgatctga cattgcgtga tgttcaggga aaaatgaaga aagccgggca
     1561 gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttatccccgc
     1621 ggcggaattt accggcgatc cacaaaatac aacgctgggc ctgagcgtaa acggcgaaaa
     1681 acgccagcaa ggtacgactg cggacatgat ccataaaatc gttccgttga tcgcttatat
     1741 gagcaagttt tttaccctca aggccggtga cgttgtgttg acaggcacgc ctgatggcgt
     1801 cggcccgttg caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac
     1861 tcgcgttttg taatactttt tgccgccaga aagcggcggc aaaacttgca tcgctgtgcc
     1921 agactggtta taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt
     1981 tctggcaaag taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg
     2041 gttgcggtca gtgttgcttg cataaactga tggatgaaga caccgacgaa atctacttca
     2101 ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc
     2161 gtttcgagtt tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat
     2221 ggctgccaat gacctgcgct tatcggttgc tggcggaagg taaagattta cctgcgtggc
     2281 atccgctact tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata
     2341 tcgcagtgaa agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact
     2401 gggcgcagtg ataaatttaa tagatgctga tgggggcttt cttaatatac gtctggatgc
     2461 tcgccatttc gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc
     2521 ttattatcaa caggtaactc tcaatcttta aaaaacgtct gtgtttttaa atgtgataaa
     2581 ccttatacct tgagtacatg gagagaataa tcgcttatca gacttcaggt acttcaaaga
     2641 gtgtcttttt accgtgtctt ttctgatact cattacaggt gttaatcatt tttttggccg
     2701 ctgcttttag caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag
     2761 tttccgtttt tatctccccg atggcggcta tttcggtggt taatttcaat ttggcggcat
     2821 cgatatcttt attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag
     2881 attttaactt gttcttcaat tctggaatca gtttcccttc aactacgccc gcagcaatag
     2941 aataggaaat gattaaacca aatggaccgg cgacgacatc ggctgcggca ccggcatacg
     3001 cttccttcct gattttatct acctgtgact ggaaatagct gcttttttct gaaaaatcat
     3061 tggttaactg gctatctaac gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc
     3121 ttaccagcag ggatttttgc gcttcattca gcttcgtgat gccgtcatcc agtaccttaa
     3181 tgaggggcgg atgctttctt ctcattgtac tcatcaaata gcaaaatata cgctgcgagc
     3241 aattgcgtcg caacaccaca ccattcatac actgtttggg ttgcttcaaa atacttatcc
     3301 tggctatcca taagtaaggt tttaatatcg ccgactaaaa cggaggctgc ctgtgaatac
     3361 tcctgtttaa agcgacttaa ctcttttatg gtttcatcaa aggtctgcca ggggatgacc
     3421 tgatcgagat atttattata aagatctaat gctccatctg cggtttcgat tgcgttttta
     3481 actacttcta ccgttttatc tgcaacgatt tcagtcataa tcattcgcct ctttaaatat
     3541 ataaattgta atgaaactcc tgttttacaa ctattaataa attttacttc atctaattca
     3601 tagttagccg ggcgggatgc gtcaatgtct ttatttctat taatatgata aatatcaaac
     3661 aatgtttaat gtcattatgg cgaatgcttc tattctattt ttttgccggg tgatattttt
     3721 catttctgct ggatgagcgt cgtcgccaga aggccacgtg agcacaggat aagagaacga
     3781 aaaatcagca gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg
     3841 caagaacaga ccactgtata taaaaacagt ataacttcag gcagattatt atgttgttta
     3901 tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt
     3961 gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg aatcaactgt
     4021 tgatccagca tcccagcgcg acttacttcg tcaaagcaag tggtgattct atgattgatg
     4081 gtggaattag tgacggtgat ttactgattg tcgatagcgc tattaccgcc agccatggtg
     4141 atattgtcat cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga
     4201 cggtacagct tattcccatg aacagtgcgt actcgcccat taccatcagt agcgaagata
     4261 cgctggatgt ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga tgtttgccct
     4321 ctgtgatgta aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg
     4381 taaaccggtg gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc
     4441 aaaggcgctg ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg
     4501 ctgtggtgtg gtttgcttta gcagcaatta tgagctttac gcagacatga gcaatcgggt
     4561 gatgtcgacg ctggaagaac tatcgccccg cgtcgagatt tacagtattg atgaagcatt
     4621 ctgcgatctg acaggtgtgc gtaattgtcg cgatctgact gattttggca gagaaattcg
     4681 cgcaacggtg ctacaacgta cccatcttac tgttggtgtg gggatcgccc agaccaaaac
     4741 gttggctaag cttgccaatc atgcggcaaa aaaatggcag cggcagacgg gtggggtggt
     4801 ggatttatca aatctggaac gccagcgtaa attaatgtct gctctccccg tggatgacgt
     4861 ctgggggatt ggacggcgga tcagcaaaaa actggacgcg atggggatca aaaccgttct
     4921 cgatttggcg gatacagata tccggtttat ccgtaaacat tttaatgtca tgctcgaaag
     4981 aacggtgcgt gaactgcgcg gcgaaccctg tttgcaactg gaagagtttg caccgacgaa
     5041 gcaggaaatt atctgttccc gctcgtttgg tgaacgcatc acggattata cgtcgatgcg
     5101 gcaggccatt tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagtg agcatcaata
     5161 ttgtcggttt atctccacat ttattaagac gtcaccattt gcgctcaatg aaccttatta
     5221 cggcaatagc gcgtcggtaa aactgctgac gcccactcag gacagcaggg atatcattaa
     5281 cgctgctacg cgatctctgg atgccatctg gcaagcgggc catcgctacc aaaaagcggg
     5341 cgtgatgctg ggggatttct tcagtcaggg agtcgcgcag ctcaatttat tcgatgacaa
     5401 cgcaccgcgc cccgggagtg agcaattgat ggcggtaatg gatactctga atgctaaaga
     5461 gggcagagga acactctatt ttgccgggca ggggatccaa caacaatggc agatgaagcg
     5521 agctatgctt tcaccacgtt atacaacgcg aagttctgat ttactgcggg tcaaataaat
     5581 atagcggcag gaaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacagat
     5641 cacgtttttt cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag
     5701 cgataaaaat accgagcagc cactgcggca tttccatacc taaaaaatcc cactgacgct
     5761 cggcgcaatc gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt
     5821 ccgggaaacg aaccataaaa tcacaggtgg caaatggcga aggatagagc tgaagcatgg
     5881 tgtgctcgta agttaactgc acaccgcgga acgcactata caaccagata accatcgcta
     5941 cataacgcag cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga
     6001 ataacgcgca gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga
     6061 accacagcgc cgtcagttcc agtgccagag cagtaaacgc catcaacagc cacgcgcccc
     6121 ggccttgtga acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga
     6181 gtgcgcagtt taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca
     6241 ataggacaat tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat
     6301 caaagcgttg ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac
     6361 tcgacgcaga gcaagccgac gagtgtcagt acgagggtgt aaggcagggc catccacacc
     6421 atgcggccat aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac
     6481 gcagcctgac cgttcggcgt agcgacagag ggcagattgg taccggtatt aatggcgacc
     6541 gccagcagct cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg
     6601 ttgatataaa tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa
     6661 atatagaaca gcgacagctg agcatgttca gatgcctgca acacaaactg aataatcggc
     6721 gaaaacagtt gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg
     6781 gtgaatggca gagattcggt gaaggcttta ccgatagaat gttcatcggt gacaccggtc
     6841 aatgatgttg ccagaataat gactgacaaa ccaatcaagc caacttccgc cagatgcaac
     6901 gccagcgcag tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc
     6961 tgacgggtac gctggtggcg gctttgatcg tcaaactgtt gcaacacttc gcggactttc
     7021 tccggcagcg tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac
     7081 aggccacaaa tcagaaccgg aacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa
     7141 tgccagccag ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcattacg
     7201 ccgcctaatg cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac
     7261 tgttccagaa ccacgttgta atgcttgtcg atatgactat cgtcttgcag gtcggtgtct
     7321 tcggtacggg aagaggctac gcgatgataa ataccataaa aaccgactgc aacgctgatc
     7381 accaccgcca cgacggttaa ggcatcgagg aacgcggaga ggaacgctgc cgccacgcaa
     7441 aaagagagcg acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac
     7501 agcaacagct gtttcataaa atagataccc gccaccataa acatcaataa cagcaagact
     7561 tcaagatttg ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc
     7621 gcttcgatag ccaacagacc accggggagt agcgggtagc atttcagggc catcgccaga
     7681 gtgaaaataa attccgctac cagcaaccag cccgcgacga aagggctgat gaggaaaatt
     7741 aacgggttta cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc
     7801 aaaaaattgc gccatagcgc gcggccccag gagatctcca tgatggtttc ccttacctta
     7861 caaataatca atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg caggagtgag
     7921 gcaagtcttg atagtcaagg ggaaagagat gcggaaaatg aagccttgat ccctttttct
     7981 tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg
     8041 ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa
     8101 gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca
     8161 gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag
     8221 cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat
     8281 ttctgggaaa cttccggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt
     8341 gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac tatttttatt
     8401 cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa
     8461 gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg
     8521 tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg gctgtatacg
     8581 cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac
     8641 cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt
     8701 cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat
     8761 ttagccattc aggggcgata atcccttccg tttaaagagc aaccccctca aacgaggggt
     8821 tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca actcgatgct
     8881 gccgtcttcg ttttgctgtt cgagcatcac atcaaatccc cacaggcgat gcacatgctt
     8941 caggacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatat aacgcagcgt
     9001 cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact
     9061 taagttatat tgcgacgata accggttacg gatctcccga taaccttctt cattatgaat
     9121 agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga agcggaaatc
     9181 acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga aatcacgcat
     9241 cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc
     9301 ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc
     9361 atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga ccacattggt
     9421 gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg
     9481 gttaaggatg gtatagtgcc agaaggtcgc acagccttcg ttcatcacct gagtctgttt
     9541 ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga
     9601 ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg
     9661 atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag
     9721 catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt tctcttcttg
     9781 cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca
     9841 cgagtccaga agccgttcta cttcatcaac gccataacgt tcttcgcact cggtaatata
     9901 tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac tgcggaataa
     9961 gcaattgttt ttaaagaaag agttatgtcc atagcaggcg tgtgccatca ccagcgcctg
    10021 catagtaatg gtgttctctt ccatcaagta agcgatacac gggttagagt taatgacgat
    10081 ttcataggcc agcccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt
    10141 accgaatgac cagtgcggat agttaattgg catgccgacg ctggaatagg catccatcat
    10201 ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc
    10261 cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc
    10321 gctcaaacgt gtggtgtcct tattcataga atcgatcgtc gccatacacg cacctcattg
    10381 ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg ttaaaaaccg
    10441 cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac taatccgctt
    10501 ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta aagacgccat
    10561 attgccgcag agtcagggag atgtgagtca gctcaccata aaaaagccgt atgttgaata
    10621 atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact gtatctaccg
    10681 ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg
    10741 cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag ccgggggccg
    10801 ccctggaaac cagtgccgcg aatgccgggc aaatctcccc cggatatgct gcaccgtggg
    10861 cagcaccagg tgtgccttta aaagcgatta aatggatgtt ccagcgccat gcgccgctgg
    10921 cggttcgtct cgacggtacg cagtttcagt tgaaatggat gtggcaaatg ttacgtaact
    10981 gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc
    11041 gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg
    11101 ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc gatatcgccg
    11161 tgctggaaga tgccggcgta ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg
    11221 atcccgcgct ggcagaagta gcgcacaaac tgacgggcgg cctgcagtta cccaatgatg
    11281 aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag caggcggggg
    11341 ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag caaatctacg
    11401 gcgtgaagtt tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg tttggttctt
    11461 actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg aaaggctact
    11521 cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt cttgatgaaa
    11581 cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga atggcggaaa
    11641 ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc
    11701 gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc
    11761 cgatgacgcc agacggtacg ccggttgtcg ggagtacacg ctttaaaaat ctgtggctga
    11821 ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag ttgttaagcg
    11881 atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta gcgcgctaca
    11941 gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc taaggaaacg
    12001 agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag aatctgtcca
    12061 ttgtccgcca ggccgcgccg cacgcgcgcg tctggtcggt ggtaaaagcg aacgcttacg
    12121 ggcatggtat tgagcgtatc tggagcgcac tcggggccac cgatggcttt gcattactta
    12181 acctggaaga ggcaataacg ttacgtgagc gcggctggaa ggggccgatc ctgatgctgg
    12241 aaggattttt ccatgctcag gatctggaga tttatgacca gcaccgcctg accacctgcg
    12301 tacacagcaa ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca ccgttggata
    12361 tttatcttaa agtgaacagt gggatgaatc ggttaggctt ccagtccgat cgcgtgctta
    12421 ccgtctggca gcagttgcgg gcaatggcga atgttggcga aatgaccctg atgtcgcatt
    12481 ttgctgaagc ggaacatcct gatggaattt ccggcgcgat ggcgcgtatt gagcaggcgg
    12541 cggaggggct ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg tggcacccgg
    12601 aagcgcattt tgactgggtt cggcctggca ttattttgta tggcgcttcg ccgtccggtc
    12661 agtggcgtga tatcgccaat accggattac gtccggtgat gacgctaagc agtgagatta
    12721 ttggtgtcca gacgctaaaa gcgggcgagc gtgtgggcta cggcggtcgc tatactgcgc
    12781 gcgatgaaca gcgaatcggc attgtcgccg caggatacgc cgacggttat ccgcgccatg
    12841 cgcctaccgg tactcctgtt ttagtggacg gcgtgcgcac catgacggtg gggaccgtct
    12901 cgatggatat gctggcggtt gatttaacgc cttgcccgca ggcggggatt ggtacgccgg
    12961 ttgagctgtg gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg
    13021 gctatgagtt gatgtgcgcg ctggcgttac gcgtcccggt tgtgacagtg taacttgttg
    13081 taagccggat cggaggcagc gtcttctggt tgcgaaaaaa tcatccatcc ggctggtcag
    13141 caactgtagt tgttaatgtg acagagccat tgcccatgat agtgtccatt aaaagaatgg
    13201 acactatttc cccggaacct gaactcgccg cacaggcgtt ctacataaaa cgcttacgct
    13261 tcattgttga ctcggagtcg acttcagata aatcacgctt cacccttgat gcagattcgg
    13321 gttcatgttc agattgttga ttaacaggct gcgagtcctg ttgtctttcc agactcaaca
    13381 ggcccaactg ttgatccagg agctccaggt cctcctgtac cttcacgcgc tccaggagtt
    13441 ccgggtcatc caggaactcc agatccataa ggtcctccag ctcctcgagt ccttccaggt
    13501 caacctagcc ttccttgtgc aaaaagtcca aaaggtcctt caggccctgc tgttttttca
    13561 ggccctcgac ggctttgagc agcgtcggta aattgtcttt gtcccacggg ttaccattac
    13621 agtcaaacac cttcaggctc tggggctgcg gcacctgtac cgttttaaac ttgttttctc
    13681 cacagtagta aattattagt ttgggcggca gccgtggtga agagatcaaa tcgttactag
    13741 aacagtcaaa tactttcagg cgagtcaagt tctccagcat ctcatcaggt attgagttaa
    13801 tattgttgcc ggcacaggat agtttataca ggtactgggg catcgacggc ggtaaaaatt
    13861 gcaagaagtt ttcagaacag ttaatcactc tcaggttagt cgacagctca ggcagccaga
    13921 taatactgtt ttcatagcac ttcagtacca tcagttttga gggctgcacc ttcggtaaca
    13981 agatcaagac gttatctgta cagtcaagca ccagcagcca ctttggcatc accttcggta
    14041 acgcggtcaa cacgttttta ctgcagtaaa attcaatcag atgtggaggc aaattctcag
    14101 ggagtgaagt caatcccagg ttgctaagat tgagcatact ccgtcttgta atgagacatt
    14161 ccttcattct gcgcaatgct tctgcccggt tttcaatccg gcttgctggc aggttttctt
    14221 ccaggatatt ttccttgatt tcttccagac tttctttcag gtcttcttgg ggaatttctg
    14281 actgtttgtt ttgctggttt tcttccagga tttcgtacag gtttttttgc aggttttctt
    14341 gcaaaccatc ctgctgtata gcccattcgc accatttggc gaccatcatg aactcgttat
    14401 gttgctgatt cagacgagtc cccaacgtga tgatggtatt gttgtccacc tgtatgttca
    14461 ctttgcccgc gatactgagg tcaatgatct gccagggggc atcaccccag ctgaattttt
    14521 gcgggctgag atacttcatg ttctgcaaaa tttgattctt gagaagagcc gccctggcgg
    14581 gcgtcgtacg cctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc
    14641 tttaaagcta gccataatgc tacgtttcct tgaaataaat atcctctggc agcgctggag
    14701 acgtgaatgt ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc
    14761 atcgttgact ttttccctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg
    14821 gcctaggtag ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt
    14881 tattaagcaa aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga
    14941 aacgcctatt gtgtgggcta tgtcagtgcg gcaggttgcg tgggggaata tgttcaggcg
    15001 atgtcaggca ggagcccctg atggttgacc ggtaatgcgg ggctggtttt tattacctgt
    15061 tacagtcgga aaaagccctt acgctgactt tcttgagccg gtgttttgag ttcattagcc
    15121 agagcctgca atggtttgtc atccataatt taaccttctt ctgatgttgg gatgagcata
    15181 tcaaaatcag gaaatgacaa gtttattaca ggctcaggct gcgttcagta aagcatcgca
    15241 tccggcgata gatgctgcat taaatttaag attcagcttc ctcttcagcc acccgaacgc
    15301 caatcttcaa cacttcatta tcttctttct cggccaccgt ccagatcatc ccggcaaact
    15361 ctacctggtc accgacaacc ggtgccgcgc ctagcaactg ctggacaatt tcacccagcg
    15421 tttgctgttt atcacgatat tctcgcccgt cttccaggcc atatatcagc gcaacatcag
    15481 catatttggc gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg
    15541 gtggcgactg gctgaacagt ttaccgagcg ccgggagatc gcgttcccga ccaattacac
    15601 acaatacatc gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac
    15661 gaaacagggc tgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt gccgcaccta
    15721 cacaccattt atcagcactc aattgataaa caaactgctc ccacggattt tccggatgaa
    15781 tatccaggcc aacgcgtgac accggacgtc cgacgggcgg aaccaccact ttggcttttt
    15841 tcgccgccca cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg
    15901 cgacattaaa gaacagtcgc gcattctcca gccccgccat catcgggaac acggctagga
    15961 tgattggcac cgcgccgcgt aatcccaccc agctgataaa cacgcgctca cgcagattga
    16021 aaccgcggaa agggagcaat ccagcaaata ccgaaagggg acgggcgaag aatatcatcc
    16081 atgccgacaa aatgagcgcc ggaatggcaa taggcagcag atcgcttggg ttaaccaata
    16141 gccccagcac caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca
    16201 ggatgccgta gcggttgcga atcggccgat tacccagcag aaaaccgcac agataaacag
    16261 ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta
    16321 atgccagcaa tggatataat ccggcaggca gggcgatgcg attaatcatt tgcagcagta
    16381 aataaccacc gccaagaccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacca
    16441 cgaacatcca actgacactg ctttcatgct gctggatcat tgcaatcagg gtaatcgtca
    16501 gaaagaccgc cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt
    16561 cgttaagccc cttaccaccc agcagagaaa agaccgctgc agcatcggtg gagccgacga
    16621 tagcgccgat taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc
    16681 cggttaaacc agaggtgata agtacgccca gcgtcgccag cgacagcgcc ggtcctaacg
    16741 ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagcg
    16801 ccaggttact taccatgtag gcgaaggggt aattatcaaa cgggataccg ccgacgccat
    16861 cgactcccgc cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg
    16921 aagaaaatga actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc
    16981 taattattgt tgtggcatcc aacggtcggt tactcctgat tacgctgtct cttatataaa
    17041 ccctaccata ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac
    17101 attttaagaa caggatgacc gctaatcgtc aactgagcgc cttcccgggt gttattcaga
    17161 atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg
    17221 gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa
    17281 cctgcgtcat aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga
    17341 cgtggcaaaa ttcccgcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc
    17401 acgttaatat cttccagcac cagaataccg ttctcaattt ttggcatcca cggtgtacca
    17461 atcagtgaaa tcagcatcgc aagattgcct ccccacaacg tgccttcggt ctgacatgtc
    17521 gggccttccc cttgccattc aatggtgaag gtttcattgc gtaacgccag ccagaaatgg
    17581 tgctcggtaa aggcgttcag ttcatccgca ccaaagtttg ctaccagcat ggggccgcta
    17641 aaggtgatga cattgccttg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt
    17701 ccgcaaatga gtaacgggtc atgttgttgg cgagccacca gtgcctgcca gtcaatatct
    17761 gccagtaaac gactggcacc gtaaccgccg cgtacagcca gcacgatggt gttgggggta
    17821 gtcagtctgg caagggaatt gagatcttcc agacgctccg tttccgtacc cgcaaaacgc
    17881 tcacaacgac gggcaataac ctcgacgtta ttgacttgat gccccgcgtc ggtaaggcgc
    17941 tggataccac gcagcgcggc gtgctgttta atgctgtaac ccgatggggc aattaagtga
    18001 aacagagaca tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag
    18061 gtgtgccgtt gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga
    18121 gatggtttgc ctttttgatt gtgttattag cgggttgttc atcaaagcat gactatacga
    18181 acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc
    18241 agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt atcgctatcg
    18301 aatcgggtgg taatccgaac gcggtgagta aatcgaatgc cattggtttg atgcagttaa
    18361 aagcttcaac ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga
    18421 ccagcgagct gaaaaatcca gagcgtaata tttcaatggg ggcggcttac ctgaatattc
    18481 tggaaaccgg cccgctggca ggcattgaag atccgaaggt actgcaatat gcgctggtgg
    18541 tgtcatacgc taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg
    18601 cgatcagcaa aatcaacgat ttggatgctg acgagttcct cgatcacgta gcgcgaaatc
    18661 atcctgcgcc gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt
    18721 aaatcagtcg cgcactttgt ccgctttttc ccgggcttct cgctcgagag agaaaataat
    18781 ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg
    18841 agtggtgatg gtttcattct tgccatcaat cactttgcgc tcgctggtgg agattaactg
    18901 ggcgtcaaag tgaaaaacac cccattgccc catgttgact tcaatttgag cgaagcgcat
    18961 accttcttgt aattcggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga
    19021 caaatcatac aagcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata
    19081 aggcggatgg agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accataaggt
    19141 gggaggcggt acggtaataa atgccggaag ctgcaagtat tcactctgct gtagttgttc
    19201 aacagtaaac tcgactttcg caccctgagt ttcggcggta atggtaatgt gctgtgcctt
    19261 tagcacggcg atgttgtctt cggcttgact gccgaaatcc agcaccagtt tatccggggt
    19321 tattgccagt attttgctga tcagctgccc gccattccaa ctgagacgca aaggaattgc
    19381 ggctttgtgc aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg
    19441 ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg
    19501 tcatagttaa caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc
    19561 agccgacaaa cgcccagcat cgatcccgcc cttgcctata cttagagcgt tgacgtaagc
    19621 atttcttgcg accgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta
    19681 tttttggcct gatagccggc attatcggca agctaatcat gccggggcgt gatggtggtg
    19741 gatttttcct gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga
    19801 ccatgtttgg cattgacggc tccatcagcg gttttaattt gcacagcttc ctggtggcgg
    19861 tggtgggcgc tatcctggtg ctgggcgtat tccgcctcct gcgaagagaa taagattttc
    19921 ataaggcgga tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgac
    19981 acctgcccag aaattgcgac cttcttcaat aaacccttca ctgtaggcat attgggtatc
    20041 aaacaggtta ttgaccgacg cattgacgct gaagccatga cctaaggtgt aatcggctcg
    20101 aatgtgggtc accgcaaaac cggcggcttt ttgtgaaccg tcactgttgc tgtagctgga
    20161 ggaacgcgcc tcttccgaca gcgttacgct taacggctcc catggtttga gtgtcatcca
    20221 tgcggtcatt gtctgcgttg gaagatcggt tatcttgccg atgtctttgc gtttggcgtc
    20281 agcgtggatc agagcgtagc tcaatcctac atccagtata ttgctgattt tccccttaat
    20341 accggcatcc agaccgctgt aatccaccgt gccgctgttc tgattttgaa tggtatcggc
    20401 attgatattg tgcgagagga tggcatcact cacccggtta tagtaaacgc tgacctcaaa
    20461 tccccagtcg cgcgtgaagg caccattcca ggttaaatcc accccgcgag cgcgttccgg
    20521 cttgagctgc gggttaacaa tcgctatctg gttatacgca ggtttggatg tggtatagcg
    20581 ttctttcagc gtcggaaagc gtgtgcggtc atagtacgaa agcgccagag tgtcttcatt
    20641 ggcaaagtgg tatttcccca tcacctgcca gttaaaagct gactgattgt tgtcgtcata
    20701 atgggtgatg ctgccatctt tctcgtgttt tttcgcttct acgctatcgc gccagtcata
    20761 gctgattccc gccacgacat cgacattatc agcagcagcc cattgatatt cactggcgag
    20821 cgaccaggta cgatcttcat agcgatcgta agcggcgtgc ggcgcacctt tttcccggtg
    20881 tacgtcatct ttccagttaa cggcaaacga cagcagatcg ttttcacgca catcggctgc
    20941 cagttgtaat ccggcaccgt cgctgtaatc ggaataatgg ctgtagctgc cttttttatt
    21001 tttcaaatca gccagcgagt tgtacatcat cagcgtattt tcaaaggtgt cgcgatacag
    21061 ccgacttttc agggtaaaac ggtcgtttag ttgggtcgct ccctgataat aaaaactttc
    21121 tttgtcatac tctggccact gccagtagcg tgatttttga ccacaatttc cgctgtatgg
    21181 cgggttatct ttttcaccat cctgcttaat gtaagtcagc gtgtattcat cgttttcgcg
    21241 cggtgtaaaa ccgagcttca caatgccgcg tttatcatca gccgatgaat taatcatctt
    21301 gccgtgtttg cctgcaatat cattatttac accatgcggc aggccgagaa aatcctgctt
    21361 tagctggcta ccgctgactt gcaaataccc aagatcgctg ctggcggcaa atgaagcatg
    21421 catatcgtag gcattgtcct gactacggct ccatccctgg cgatatccca gacttgcttc
    21481 ttcagggtgg tgatattaat ggctccgccc atctgattag gcccatgaag cagcgacgaa
    21541 taccctttgg aaacttcgac tgcccccaga ttgttggtca gaatccgcgc cagatcgagg
    21601 ttgccgtcat aggggacata aatgggcaca ccgtcgaaat agaccggcac ctgccgacta
    21661 tcaaagccac gaacttttac ctgttcttcg ttgcggctgc ctgacttttg cagcaccacg
    21721 ccggggacga cacttaatgc ctgagcgaca ttctgtttat ccaaggcctt catggtgggt
    21781 tgatcgagaa cggtggtcgt cgtcgatgat accggactgg accatacggt cagcgtgtcg
    21841 ctctcctgag ccgctacggc ctgctgggta aaggcaagtg ataatgctgt gcagagataa
    21901 cgttttttga gtctcataga ataaattccg tttaacaaag aattaaatag aggctgtggg
    21961 ttgcagactg actttaatgt cagctggtgc cgcgtaataa ggcgcggagg taataaaaag
    22021 gcgaatgccg cagtcgaggt aatttttgag tgtcgtcaga ttaattccgc cggtcagagc
    22081 cagcgtgcag tgaggagcca gcgacggcgc tatttgcgca atttcagttg cctgttgcgg
    22141 actaaatttg tcgagctgaa gtacgtctgg ttgtgcgcgt aacgcggcga tagcctcttt
    22201 cggcgtgtcg gcttcaacaa caattttctt ctcgggtgcg tggcgacgta actgagcgat
    22261 tgcgcctgac cagccctgat tgtcatgaag aaaatggcgg tggttggcaa acaataatat
    22321 ggtttccgca catccggcac gatgaattag tcctccggca gccagaattg cctgcgaagc
    22381 cagtaagcgg gtgcccggaa ttgcttttcg ggtgcaggcg atattaccgt caggataacg
    22441 ttcacgaagc aatgccagca tttgagcgag ataatcagaa acaccgcaac tccactccag
    22501 cacattctgg actgccttcc atccctgatg aagtgctgcg gcattaccct gcgcacggat
    22561 tagccgttga cccgcgttcg cttgtgaacc gtcgctgacc gcgtcatcaa tagttaatcc
    22621 cagcgtagtt aacatcttac acgcgacaga aacgccgctg acgcagccgc cctgacggtg
    22681 aaaaaacgct atatagccag gctggtgtcc aatatttaat gctcgcgtgg tcaggtcacc
    22741 tccctggata tcttccagca gtaacgcatc gatttgtgcc tgagaaagga aaatcatcac
    22801 agtgcctcct gcggtacgct atcccaccag atgagcgccc agtcgcggct ggcaggggcg
    22861 agggcacgag catcttgttg ttgataccag tgatataacc gctcacgctc atcgtcgtta
    22921 agcgcgccaa gactccagct gacgttctgc tcaaaacgtt cccaggtgct gttatcctgt
    22981 tggcaatttt gtccacggat aaaatcaacg tgggcataaa ttcccatctg atacaggacg
    23041 ttgagcgcaa agatatagtt gggtaattcg atcacttccc gaccagccgc ccgctgaatg
    23101 gctggagaga cgaacgaggt gctgaccaga tgagttgtat agacgcgaag ccgtgcctga
    23161 ttattcagtt tgctcattgc atggcgcata tcagccacca gagtggagcg ggaagccacg
    23221 gcaatatcgc agcgtggcag gtcactccag tcctcttccc aggcgcgctg gatccagtgg
    23281 acattgtcag ccttcagtgc ggcggcgcgg cgggcggcaa cgttcagcat cccctgactg
    23341 taatcaacgc cataaaccgt agtgagtttg tcagccagcg ccagcgatac ggtgcctggt
    23401 ccgcagccca tatcgaataa ggtctgagcg ccttgcaggt caattttagc gatcagttgt
    23461 tgcaggtagc tgtccgttgg cgaggcgcaa ttttccgcca ttttctccgc ccgttgatcc
    23521 cagtgatcgg gcgttttttc tgtgcggtga gccagtttga gttgctgcag ataaaggtct
    23581 gcaaaatcaa tatcatcaat gagcatgatt gttccttagc ggctaattgc agagaggtga
    23641 tgatgaattt ggtcggcaga aacccgatac agggccgcca gtttgttcgt tgttaactgt
    23701 tcggtcggta agccttgtgt tactcgtcca tctggctcta cttggataat gctgtcggca
    23761 atggcgttgg catggagcgg atggtgagtc gacattaaca ttgtcatgcc gttatttttg
    23821 agctgcgcca gcgtgtccag aagttggatc tgatgaccga aatcgaggct ggaggcgggc
    23881 tcatccagta acaacaagcg cggttgttgg acgagtgcac gggcgattaa agccagctga
    23941 cgttcaccgc cgctaagcgt attccagcgt cgtgtcgcca gatgcaaaat gcccagcttt
    24001 tccagttgct ccgtggcttt caggcgttct tgcttaccgg gaacggaaaa agcgcctatg
    24061 gtgggggcca ggcccatcaa caccatatcc agcacactga aagcaaaaat accgtcgtgc
    24121 gcctgcggca cccaggcaat agcctgtgcg cgctggcgat ggctgagtgt ctgcacagag
    24181 aggctatcca gtaggatctc gccttttaat aacggtataa cgccaagaat ggagcgcatg
    24241 agtgtggttt tgccgcaacc attcgcgcca agcagacaac aaattgttcc ttgcgacagg
    24301 gtgaaggaca cgtcgcgtag cactggatgg cgggagtaac ccagagacgc atgacgaacg
    24361 gcaagcaaag tcactggcga cctccgcgta acaacaaaag caggaaaaac ggtgcgccaa
    24421 caaaagcggt cagaatgcca agtgggattt ctgttgttcc aatgctgcgc gccagcgtat
    24481 cggtaagcag gagcagaata gcaccagtac acattgccat aggcaacagc tgctgatggt
    24541 tatgaccggt caacaagcgg gcaatgtgcg gaacgaccag accgatccaa ccaattatgc
    24601 ccgctattgc taccgtgctg gcggtgatta gtgtggcgca gacaatcaat ccaaagcgca
    24661 gacgcgtcac gttcagtccc agcgaacggg cttcgtcgtc ggaaagcgtg agtaaattca
    24721 tgcgccagcg gagaaaaaac aggggcaagg agcctatgag aatgatcggt gcagcatagc
    24781 agagatcgcg taaggtcacg gtggacagtc cccccagcaa ccagaaggta attgacggta
    24841 actgggtgta aggatcggcc agcgttttaa ttagcgatat ccccgctccg cacaacgttc
    24901 ctaacgcaat gcctaccagc accagggtta agatcggatc gtggcgggtc acccgacgcg
    24961 taatcaacca gacgcccgcg accaccatca gcccgccgca aaatgcgtac agctggatgt
    25021 aaacaatggg taatccccac aggattgccg cgcaggcacc aagtccggca ccggcggaga
    25081 cgcctaaaat atccggtgaa actaagggat tacgcaacat tccctgatag gtggttccgg
    25141 ctcctgccag cgctgcgccc agtaacagcg cagcaagaat acgcggcacg cggatttgcc
    25201 agaagacaat ttgttcctgc acagactcat tttcttgacg aagaattagc gccagcagtt
    25261 gctgcatatc gagatgataa gccccactta cggcggcaaa agtgatactg atagctgcca
    25321 gcagcaaaat acatccgttt acgattctca ttagctcgcc accaattttt gatagtcggc
    25381 atcactgagt gaacaatgcc agaacagttg ggcgtaatgc tgcatgtcac ttttaaactg
    25441 gcgattgatg gcgggatcca gccaggcgtg aagtctgcgt aatcccagaa gacggttgat
    25501 tcccggcggg gcatcgagcc agccaaaggg caagccactt aaaaataaga tgcgttgttc
    25561 cgcaacggct ttcacgccct gccagagagg atcacgacga ataaaatctg cagtaacggc
    25621 ctcctgaacc agaataatat ccggctgcca gcgcaggaga ttttccatgg aaacctgtgt
    25681 caggccgtgg cgatcggcta tttgcgcgac gttgtgcaaa cctaataatt ccgccgcctc
    25741 ggtatgcaac gaaccctgta agcccgtttc cagccctcgg ggaccgcgcg cagcataaaa
    25801 gcggaggtta gcggcgggtg aggtggcgaa tgcctgagct tcaccaacga agtggctggc
    25861 gagattggct tgttctgcgg cgcggtgctc ttcgcctaac gttttgccaa gcgttgttaa
    25921 ctgttctgct gattgcgcca gttttccgtt aagcaataac caggggattt gtgtctgttc
    25981 gctaacctgc cgtgcctggg agatcaaggt ttcatccgta ttgccgcaat caacgaccaa
    26041 atcgggatgt aacgccataa gcccttccag cgatagtgtg ctggcgcgcc cggcgagtcg
    26101 tcccagcctg ggtaactggc gaatgtgctc tggcaatgga attaatgcct gacgggcaaa
    26161 atcaaaagag gaaaaaccga ccattttttc tggcgcgaca gccagcaaga gcaaatcggc
    26221 gggcgcgcct gcgctgacga tgcgtgtaat gttttgggct tcgggaagag attgcgaagc
    26281 aaaacgatta actgtttgtg aaaaggacgg tagagactgc aggagcaggg tagatgcaag
    26341 tgcctgcgca aacgtccgtc tggttatggg catggagaac atcctgaaaa gtaaaatcga
    26401 tatatacttt catatatatc gataaaaatc atgttcaata tcaaagttta gctattgaaa
    26461 acatgaagat gtgacgactt ccgcaagcct gttgaccctc tctatcatta aaaatgtatt
    26521 tatcaggtgg ctaatattat ttaaatcatt taaggctaaa tcatttaaat atgaatgaca
    26581 ttattgtttt ttatcgtcca cggttacgcg acgttgaagt tacttgtttt ataatgagcg
    26641 atttttgata tctcgccgag aaaattatca gcattctctc gtttcagtgt cgtgaatata
    26701 ctgttaacgg aggtgaaaag aatgctgggg gttatacaaa acctatgttg ttgtcgtccg
    26761 gattcaccgc ttgctgatag taagcagcgt aaccagaagt taccgcaaca agactttgag
    26821 gattttaaga acgacgttat taatgtcatt aacaacaatg aagatattta ttttcttaga
    26881 gagagtaatt tactgaatgc gtttattcgg acgtgcaaaa aaagagatga taacagttac
    26941 ggaaatatga tatttacgct ttattttgat aacgatatct atgggaacag aattatcaaa
    27001 gcatcgctga acggcggaga gttgcagtta ttaagcacag ggtgtagcaa tgataacatt
    27061 ctccat
//
LOCUS       DAECNO010000055        26076 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.055, whole genome shotgun sequence.
ACCESSION   DAECNO010000055 DAECNO010000000
VERSION     DAECNO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.055"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KKO08_002336"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="KKO08_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBD6318561.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="KKO08_002337"
     CDS             complement(2138..2584)
                     /locus_tag="KKO08_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBD6318562.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="KKO08_002338"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="KKO08_002338"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBD6318563.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KKO08_002339"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="KKO08_002339"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBD6318564.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="KKO08_002340"
     CDS             complement(5347..6009)
                     /locus_tag="KKO08_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBD6318565.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="KKO08_002341"
     CDS             6182..6595
                     /locus_tag="KKO08_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBD6318566.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KKO08_002342"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="KKO08_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBD6318567.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KKO08_002343"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="KKO08_002343"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBD6318568.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="KKO08_002344"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="KKO08_002344"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBD6318569.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KKO08_002345"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="KKO08_002345"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBD6318570.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KKO08_002346"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="KKO08_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBD6318571.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="KKO08_002347"
     tRNA            complement(9861..9948)
                     /locus_tag="KKO08_002347"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="KKO08_002348"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="KKO08_002348"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBD6318572.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="KKO08_002349"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="KKO08_002349"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBD6318573.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="KKO08_002350"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="KKO08_002350"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBD6318574.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="KKO08_002351"
     CDS             14005..14592
                     /locus_tag="KKO08_002351"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBD6318575.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="KKO08_002352"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="KKO08_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBD6318576.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="KKO08_002353"
     CDS             14984..15841
                     /locus_tag="KKO08_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBD6318577.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="KKO08_002354"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="KKO08_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBD6318578.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="KKO08_002355"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="KKO08_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBD6318579.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="KKO08_002356"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="KKO08_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBD6318580.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="KKO08_002357"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="KKO08_002357"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBD6318581.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KKO08_002358"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="KKO08_002358"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBD6318582.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KKO08_002359"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="KKO08_002359"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBD6318583.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="KKO08_002360"
     CDS             complement(22898..24037)
                     /locus_tag="KKO08_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBD6318584.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25243)
                     /locus_tag="KKO08_002361"
                     /pseudo
     CDS             complement(24083..25243)
                     /locus_tag="KKO08_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(25322..26017)
                     /locus_tag="KKO08_002362"
     CDS             complement(25322..26017)
                     /locus_tag="KKO08_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6318585.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6648 c         6906 g         6305 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcgga
//
LOCUS       DAECNO010000056        25535 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.056, whole genome shotgun sequence.
ACCESSION   DAECNO010000056 DAECNO010000000
VERSION     DAECNO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.056"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            49..1388
                     /gene="yjeM"
                     /locus_tag="KKO08_002363"
                     /pseudo
     CDS             49..1388
                     /gene="yjeM"
                     /locus_tag="KKO08_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="KKO08_002364"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="KKO08_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBD6318586.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="KKO08_002365"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="KKO08_002365"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBD6318587.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="KKO08_002366"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="KKO08_002366"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBD6318588.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="KKO08_002367"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="KKO08_002367"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBD6318589.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7840..7915
                     /locus_tag="KKO08_002368"
     tRNA            7840..7915
                     /locus_tag="KKO08_002368"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7873..7875,aa:Gly,seq:gcc)
     gene            7951..8026
                     /locus_tag="KKO08_002369"
     tRNA            7951..8026
                     /locus_tag="KKO08_002369"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7984..7986,aa:Gly,seq:gcc)
     gene            8259..8312
                     /locus_tag="KKO08_002370"
     CDS             8259..8312
                     /locus_tag="KKO08_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318590.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8295..9434)
                     /gene="queG"
                     /locus_tag="KKO08_002371"
     CDS             complement(8295..9434)
                     /gene="queG"
                     /locus_tag="KKO08_002371"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBD6318591.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9433..10980
                     /gene="nnr"
                     /locus_tag="KKO08_002372"
     CDS             9433..10980
                     /gene="nnr"
                     /locus_tag="KKO08_002372"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBD6318592.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            10952..11413
                     /gene="tsaE"
                     /locus_tag="KKO08_002373"
     CDS             10952..11413
                     /gene="tsaE"
                     /locus_tag="KKO08_002373"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBD6318593.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11432..12769
                     /gene="amiB"
                     /locus_tag="KKO08_002374"
     CDS             11432..12769
                     /gene="amiB"
                     /locus_tag="KKO08_002374"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBD6318594.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            12779..14626
                     /gene="mutL"
                     /locus_tag="KKO08_002375"
     CDS             12779..14626
                     /gene="mutL"
                     /locus_tag="KKO08_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBD6318595.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14619..15569
                     /gene="miaA"
                     /locus_tag="KKO08_002376"
     CDS             14619..15569
                     /gene="miaA"
                     /locus_tag="KKO08_002376"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBD6318596.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15655..15963
                     /gene="hfq"
                     /locus_tag="KKO08_002377"
     CDS             15655..15963
                     /gene="hfq"
                     /locus_tag="KKO08_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBD6318597.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16039..17319
                     /gene="hflX"
                     /locus_tag="KKO08_002378"
     CDS             16039..17319
                     /gene="hflX"
                     /locus_tag="KKO08_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBD6318598.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17405..18664
                     /gene="hflK"
                     /locus_tag="KKO08_002379"
     CDS             17405..18664
                     /gene="hflK"
                     /locus_tag="KKO08_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBD6318599.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18667..19671
                     /gene="hflC"
                     /locus_tag="KKO08_002380"
     CDS             18667..19671
                     /gene="hflC"
                     /locus_tag="KKO08_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBD6318600.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            19753..19950
                     /locus_tag="KKO08_002381"
     CDS             19753..19950
                     /locus_tag="KKO08_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBD6318601.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20054..21352
                     /gene="purA"
                     /locus_tag="KKO08_002382"
     CDS             20054..21352
                     /gene="purA"
                     /locus_tag="KKO08_002382"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBD6318602.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21557..21982
                     /gene="nsrR"
                     /locus_tag="KKO08_002383"
     CDS             21557..21982
                     /gene="nsrR"
                     /locus_tag="KKO08_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBD6318603.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22021..24462
                     /gene="rnr"
                     /locus_tag="KKO08_002384"
     CDS             22021..24462
                     /gene="rnr"
                     /locus_tag="KKO08_002384"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBD6318604.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24642..25373
                     /gene="rlmB"
                     /locus_tag="KKO08_002385"
     CDS             24642..25373
                     /gene="rlmB"
                     /locus_tag="KKO08_002385"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBD6318605.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            25474..>25535
                     /locus_tag="KKO08_002386"
     CDS             25474..>25535
                     /locus_tag="KKO08_002386"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318606.1"
                     /translation="MASISIRCPSCSATEGVVRNG"
BASE COUNT         5963 a         6482 c         7157 g         5933 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagtacg cgggaatagc tcagttggta
     7861 gagcacgacc ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg ctccaaaatt
     7921 taaaagtgct gtaaggcaca gaccacccaa gcgggaatag ctcagttggt agagcacgac
     7981 cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc gctccaaatt cttctctcaa
     8041 taaaatatct acagcgacgc gatgcgttat tgctggtttt tgttgtctct gacaaactct
     8101 tgtaaacaga gttatccaca gcctcaggct gtaatcttaa tttcaaagaa acttcgcacg
     8161 gtgaatggta ttttttaacc tattgataaa taagttaaaa atcaagattc cgttttgtcg
     8221 agtcgatcac ttgacgattt tattcgtctt gaattgcaat gcgtttttat ttttattcac
     8281 aagctgtgga tgaatcaggc gtcacgcggt aacccttttt caatcacccg aaccagacgc
     8341 tgttttttcg gcaattgcac ttcgactatg cacgcatttc gtctctctat ttgctgcgca
     8401 atcgcccacg ctatgtgctc atcgagaagt gggtgctcac ctttacgact ttccagcgct
     8461 gtcaaaatcg tttcatccca aggggcattg cctaatgcca cggcgatatt acgcagccag
     8521 cgcaggtgac ctatacggcg aatcgccgat ccttccgtga cttttaaaaa cttctcttcg
     8581 ctccaggcaa ataactcaat gagttccggt gcgtgtagcg gcttacgcgg gctgaaatcg
     8641 tcttctgtag tgagttgcga atagcgattc cacgggcaga taagctggca gtcatcgcaa
     8701 ccgtaaatac ggtttcccat taacggtcgc aactcttccg ggagcgcccc ttcaagttcg
     8761 atggtgagat aagagataca gcggcgagca tcgacggtat atggctcgac gatggcaccg
     8821 gtcgggcaaa tcgtcataca ggccacgcat ttgccgcatc cttcctcgac tggttgatcc
     8881 acgggcagcg gaatatcgac cagcaattcg cctaaaaaga agaacgaacc ggcctcgcga
     8941 ttgaggataa gtgagtgctt acctgtccag ccgagcccag ctttttcagc taacgggcgc
     9001 tcgagaatag gcgcagaatc gacaaacggt ctaaaattca gcgaaacaca atgttgctga
     9061 atcatctcgc ccagcttttt gagtcggttg cgcagaagtt tgtgatagtc acggcccagc
     9121 gcataacggc taacatagcc gagtttgggg tttttcagcg tgctggcaaa tgcggcgtta
     9181 gcaggaaggt aattcatccg cacgctgatt acacgcagcg taccgggtaa taactcatgg
     9241 gggcgagcgc gcagcatacc gtgacgtgcc atccagtcca tttcgccgtg gtattgtttg
     9301 tccagccatg cttgcagttt gggctcggac tcgctgagat cggtatcggt aatacctacc
     9361 tgctgaaagc ccagttccag cccccactgt ttaatttttt gcgctaactg attgagatcg
     9421 aggggctctg acatgacgga ccatacaatg aagaaaaacc ccgtaagtat accacacacc
     9481 gtctggtacg ccgacgatat ccgccgcgga gaacgcgagg cggcagatgt gctggggctc
     9541 acactctatg agctgatgct tcgcgctggc gaggccgcat tccaggtgtg tcgttcggcg
     9601 tatcctgacg cccgccactg gctggtgctg tgcggtcatg gtaataacgg cggcgatggc
     9661 tacgtggtcg cgcgactggc caaagcggtc ggcattgagg tcacgttgtt ggcccaggag
     9721 agcgacaaac cgttgccgga agaggccgcg ctggcacgcg aagcatggtt aaacgcgggt
     9781 ggcgagatcc atgcttcgaa tattgtctgg cccgaatcgg tagatctgat tgttgatgcg
     9841 ctgctcggta ccggcttgca gcaagcgccc cgcgaatcca ttagccagtt aatcgaccac
     9901 gctaattccc atcctgcgcc gattgtggcg gttgatatcc cttccggcct gctggctgaa
     9961 accggcgcta cgccaggcgc agtgatcaac gccgatcaca ccatcacttt tattgcgctg
    10021 aaactaggct tgctcactgg aaaagcgcgg gatgttacag gacaactgca ttttgactca
    10081 ctggggctgg atagctggct ggcaggtcag gagacgaaaa ttcagcggtt ttcggcagaa
    10141 caactttctc actggctgaa accgcgtcgc ccgacttcgc ataaaggcga tcacgggcgg
    10201 ctggtgatta tcggtggcga tcacggcacg gcgggggcta ttcgtatgac gggggaagcg
    10261 gcgctacgtg ctggtgctgg tttagtccga gtactgaccc gcagtgagaa cattgcgccg
    10321 ctgctgactg cacgaccaga attgatggtg catgaactga ctatggactc tcttaccgaa
    10381 agcctggaat gggccgatgt ggtggtgatt ggtcccggtc tgggccagca agagtggggg
    10441 aaaaaagccc tgcaaaaagt tgagaatttt cgcaaaccga tgttgtggga tgccgatgca
    10501 ttgaacctgc tggcaatcaa tcccgataag cgtcacaatc gcgtgatcac gccgcatcct
    10561 agcgaggccg cacggttgtt aggctgttcc gtcgctgaaa ttgaaagtga ccgcttacat
    10621 tgcgccaaac gtctggtaca acgttatggc ggcgtagcgg tgctgaaagg tgccggaacc
    10681 gtggtcgccg cccatcctga cgctttaggc attattgatg ccggaaatgc aggcatggcg
    10741 agcggcggca tgggcgatgt gctctctggt attattggcg cattgcttgg gcaaaaactg
    10801 tcgccgtatg atgcagcctg tgcaggctgt gtcgcgcacg gtgcggcagc tgacgtactg
    10861 gcggcgcgtt ttggaacgcg cgggatgctg gcaaccgatc tcttttccac gctacagcgt
    10921 attgttaacc cggaagtgac tgataaaaac catgatgaat cgagtaattc cgctccctga
    10981 tgagcaggca acattagacc tgggcgagcg ggtagcgaaa gcctgcgatg gcgcaaccgt
    11041 aatctatctg tatggcgatt taggcgcagg taaaaccacc tttagccggg gctttttaca
    11101 ggctctgggt catcagggta atgtcaaaag ccccacttat acgctggtcg aaccctatac
    11161 gctcgacaac ttaatggtct atcactttga tttgtaccgc cttgccgatc ccgaggagct
    11221 ggagtttatg gggatccgcg attattttgc caacgatgcc atctgcctgg tggagtggcc
    11281 acaacaaggt acaggtgttc ttcctgaccc ggatgtcgaa atacacattg attatcaggc
    11341 acaaggccgt gaggcgcgcg tgagtgcggt ttcctctgcg ggtgaattgt tgctggcgcg
    11401 tttagccggt taacctttga aaggtggcgg gatgatgtat cgcatcagaa attggttggt
    11461 agcgacgctg ctgctgctgt gcacgccggt gggtgccgcg acgctctctg atattcaggt
    11521 ttctaacggt aatcaacagg cgcggataac gttgagtttt attggcgatc ctgattatgc
    11581 gtttagccat caaagcaaac gcaccgtggc gctcgatatc aaacaaacgg gcgtgattca
    11641 gggactgccg ttgttgttca gcggcaataa tctggtgaag gcgattcgct ctggaacgcc
    11701 taaagatgca caaacgctac ggctggtggt cgatcttacc gaaaacggta aaaccgaagc
    11761 ggtgaagcgg cagaatggca gcaattacac tgtcgtcttt acgattaacg ccgatgtgcc
    11821 gccaccgcct cctccgccgc ccgtggttgc gaaacgcgtt gaaacgcctg cggttgtcgc
    11881 accgcgcgtc agcgaaccgg cgcgcaatcc gtttaaaacg gaaagtaacc gcactacggg
    11941 tgttatcagc agtaatacgg taacgcgtcc ggcagcgcgc gcgacggcta acactggcga
    12001 taaaattatc atcgctattg atgccggaca cggcggtcag gaccctggcg ctatcggccc
    12061 cggtggtacg cgggagaaaa atgtcaccat cgccatcgcg cgtaaattgc gtactttgct
    12121 caatgacgat ccgatgttta aaggcgtttt aacccgtgac ggggattact ttatctcggt
    12181 gatggggcgc agtgatgtgg cacataagca aaacgccaat ttcctcgtgt cgattcacgc
    12241 tgatgccgca ccgaaccgta gtgcgactgg cgcttccgta tgggtgctct ctaaccgtcg
    12301 cgccaacagt gaaatggcca gctggctgga gcagtacgag aaactgtcgg agctgctggg
    12361 tggggcgggt gatgtgctgg cgaacagtca gtctgacccc tatttaagcc aggcggtgct
    12421 ggatttacag ttcggtcatt cccagcgggt agggtatgat gtagcgacca gtatgatcag
    12481 tcagttgcaa cgcattggcg aaatacataa acgtcgacca gaacacgcca gccttggcgt
    12541 tctgcgctcg ccggatatcc catcagtact ggtcgaaacc ggttttatca gcaacaacag
    12601 cgaagaacgt ttgctggcga gcgacgatta ccaacaacag ctggcagaag ccatttacaa
    12661 aggcctgcgc aattacttcc ttgcgcatcc gatgcaatct gcgccgcagg gtgcaacggc
    12721 acaaactgcc agtacggtga cgacgccaga tcgcacgctg ccaaactaag gacgattgat
    12781 gccaattcag gtcttaccgc cacaactggc gaaccagatt gccgcaggtg aggtggtcga
    12841 gcgacctgcg tcggtagtca aagaactagt ggaaaacagc ctcgatgcag gtgcgacgcg
    12901 tatcgatatt gatatcgaac gcggtggggc gaaacttatc cgcattcgtg ataacggctg
    12961 cggtatcaaa aaagatgagc tggcgctggc gctggctcgt catgccacca gtaaaatcgc
    13021 ctctctggac gatctcgaag ccattatcag cctgggcttt cgcggtgagg cgctggcgag
    13081 tatcagttcg gtttcccgcc tgacgctcac ttcacgcacc gcagaacagc aggaagcctg
    13141 gcaggcctat gccgaaggac gcgacatgga cgtgacggtt aaaccggcag cgcatccggt
    13201 gggaacgacg ctggaggtgc tggatctgtt ctacaacacc ccggcgcggc gcaaattcct
    13261 gcgcaccgag aaaaccgaat ttagccatat tgatgaaatc atccgccgca ttgcgctggc
    13321 gcgtttcgac gtcacgatca acctgtcgca taacggtaaa attgtgcgtc agtaccgcgc
    13381 agtgccggaa ggcgggcaaa aagaacggcg cttaggcgcg atttgcggca ccgcttttct
    13441 cgaacaagcg ctggcgattg aatggcaaca cggcgatctc accctgcgcg gctgggtggc
    13501 cgatccaaat cacactacgc ccgcactggc ggaaattcag tattgctacg tgaatggtcg
    13561 catgatgcgc gatcgcctga tcaatcatgc gatccgccag gcctgcgaag acaaactggg
    13621 ggccgatcag caaccggcat ttgtgttgta tctggagatc gacccgcatc aggtggacgt
    13681 caacgtacac cccgccaaac acgaagtgcg tttccatcag tcgcgtctgg tgcatgattt
    13741 tatctatcag ggcgtgctga gcgtgctaca acagcaactg gaaacgccgc taccgctgga
    13801 cgatgaacca caacccgcac cgcgtgccat cccggaaaac cgcgtggcgg cggggcgcaa
    13861 tcactttgca gaaccggcag ctcgtgagcc ggtagctccg cgctactctc ctgcgcccgc
    13921 ctcaggcagt cgtccggctg ccccctggcc gaatgcgcag ccaggctacc agaaacagca
    13981 aggtgaagtg tatcgccagc ttttgcaaac gcccgcgccg atgcaaaaac caaaagcgcc
    14041 ggaaccgcag gaacctgcac ttgcggcgaa cagtcagagt tttggtcggg tactgactat
    14101 cgtccattcc gactgtgcgt tgctggagcg cgatggcaac atttcacttt tatccttgcc
    14161 agtggcagaa cgttggctgc gtcaggcaca attgacgccg ggtgaagcgc ccgtttgcgc
    14221 ccagccgctg ctgattccgt tgcggctaaa agtttctggc gaagaaaaat cggcattaga
    14281 aaaagcgcag tctgccctag cggaattggg tattgatttc cagtcagatg cacagcatgt
    14341 gaccatcagg gccgtgcctt tacccttacg ccaacaaaat ttacaaatct tgattcctga
    14401 actgataggc tacctggcga agcagtccgt attcgaacct ggcaatattg cgcagtggat
    14461 tgcacgaaat ctgatgagcg aacatgcgca gtggtcaatg gcacaggcca taaccctgct
    14521 ggcggacgtg gaacggttat gtccgcaact tgtgaaaacg ccgccgggtg gtctgttaca
    14581 atctgttgat ttacatccgg cgataaaagc cctgaaagat gagtgatatc agtaaggcga
    14641 gcctgcctaa ggcgattttt ttgatggggc cgacggcctc cggtaaaacg gcgttagcca
    14701 ttgagctgcg taaaatttta ccagtagagt tgataagcgt tgattctgcc cttatttaca
    14761 aagggatggt tatcgggacg gcgaagccga acgctgaaga gttactcgcc gcgccgcacc
    14821 gattgctgga tattcgcgat ccgtcgcagg cttactcggc tgctgatttt cgccgcgatg
    14881 cgctggcgga aatggccgat atcaccgcgg cggggcggat cccactgtta gtgggcggta
    14941 cgatgttgta tttcaaggca ttgctggaag ggttgtcgcc gctaccgtcg gcagacccgg
    15001 aagtgcgggc cagaattgag caacaggcgg cagagcaagg ttgggagtca ttgcatcgtc
    15061 aacttcagga ggtagatccg gttgcggcag caaggattca tccaaatgat ccacaaaggc
    15121 tttcccgggc actggaagtt tttttcattt cgggtaaaac tttaacggaa ctgacgcaaa
    15181 cgtcaggaga cgctctaccg tatcaggtgc atcagttcgc catcgccccg gcgagccgtg
    15241 aactgctcca tcaacgcatt gagcagcgtt ttcatcagat gttggcttca ggttttgaag
    15301 cagaagtccg ggcgcttttt gcccgaggag atttgcatac ggacttgcct tccattcgtt
    15361 gcgtgggtta tcgccagatg tggtcttacc ttgaaggcga aatctcatac gatgaaatgg
    15421 tttatcgagg tgtttgcgcc acgagacagt tggcgaagcg gcagataacc tggctgcgtg
    15481 gttgggaagg ggttcactgg cttgacagtg aaaaaccaga acaggcgcgt gacgaagtat
    15541 tacaggttgt tggtgctatc gcaggctgaa tgtgtacaat tgagacgtat cgtgcgcaat
    15601 tttttcagaa tcgaaaggtt caaagtacaa ataagcatat aaggaaaaga gagaatggct
    15661 aaggggcaat ctttacaaga tccgttcctg aacgcactgc gtcgggaacg tgttccagtt
    15721 tctatttatt tggtgaatgg tattaagctg caagggcaaa tcgagtcttt tgatcagttc
    15781 gtgatcctgt tgaaaaacac ggtcagccag atggtttaca agcacgcgat ttctactgtt
    15841 gtcccatctc gcccggtttc tcatcacagt aacaacgccg gtggcggtac cagcagtaac
    15901 taccatcatg gtagcagcgc gcagaatact tccgcgcaac aggacagcga agaaaccgaa
    15961 taaggtttcg ggctgttttt ttacacgggg agccagcgat cctgcgttcc ccgctgatct
    16021 atttagaggg ttatacgctt gtttgaccgt tatgatgctg gtgagcaggc ggtactggta
    16081 cacatctatt ttacgcaaga caaagatatg gaagacctcc aggagtttga atctctggtc
    16141 tcttccgccg gtgtcgaagc attgcaggtg attaccggta gccgtaaagc gccgcaccca
    16201 aagtattttg taggtgaagg taaagcagtt gaaattgcgg aagctgtcaa agcgatgggt
    16261 gcttcggtcg ttctttttga ccatgccctg agcccggcgc aagagcgtaa cctggagcgt
    16321 ttgtgcgagt gtcgtgttat cgaccgcacc ggccttattt tagatatttt cgcccaacgt
    16381 gcgcgtaccc atgagggtaa gttgcaggtt gagctggcgc agctgcgcca tctggctacg
    16441 cgcctggtgc gtggctggac ccaccttgaa agacagaaag gcgggatagg tttgcgtggt
    16501 ccgggtgaaa cccagctcga aaccgaccgt cgtttgttgc gtaatcgcat cgtgcagata
    16561 cagtcgcgcc tggaaagagt tgaaaagcag cgtgagcagg ggcggcaatc gcgtatcaaa
    16621 gccgacgttc ctactgtttc gctggtggga tataccaacg ccggtaaatc tacccttttc
    16681 aatcgcatca ccgaagcgcg ggtctacgcg gcagaccagt tgtttgccac cctcgacccg
    16741 acgttgcggc gtattgacgt tgcagatgtc ggtgaaaccg tacttgcaga taccgtaggg
    16801 tttattcgcc acctgccgca cgatctggtg gcggcattta aagccacgtt acaagagacg
    16861 cggcaagcca cattactgct gcacgtcatt gatgcggcgg atgtgcgtgt acaagaaaac
    16921 atcgaagcgg tgaatacggt tcttgaagag atcgacgctc acgagatccc aaccctgctg
    16981 gtgatgaaca agatcgatat gctggaagat ttcgaaccgc gtattgatcg ggacgaagag
    17041 aacaaaccga tccgtgtctg gctttccgca cagaccggag cggggatacc acagcttttt
    17101 caggctttga cggagcggct ttccggcgag gtggcgcagc atacattgcg tctgccaccg
    17161 caggaagggc gtctgaggag tcgtttttat cagcttcagg caatagaaaa agagtggatg
    17221 gaggaggacg gcagcgtaag tctgcaagtt cgtatgccga tcgttgactg gcgtcgcctc
    17281 tgtaaacaag aaccggcgtt gatcgattac ctgatctaac ggcgtagcgt ctgaagcgtg
    17341 gagtcatatc ctctggcgtc gaaagacaac agggatcacc gcataacaaa tatggagcac
    17401 aaacatggcg tggaatcagc ccggtaataa cggacaagac cgcgacccgt ggggaagcag
    17461 caaacctggc ggcaactctg agggaaatgg aaacaaaggc ggtcgcgatc aagggccacc
    17521 tgatttagat gatatcttcc gcaaactgag caaaaagctc ggtggtctgg gcggcggtaa
    17581 aggcaccgga tctggcggtg gcagttcatc gcaaggcccg cgcccgcagc ttggcggtcg
    17641 tgtcgttacc atcgcagcgg cagcgattgt cattatctgg gcggccagtg gtttctatac
    17701 cattaaagaa gccgaacgcg gcgtggtaac acgctttggt aaattcagcc atctggttga
    17761 gccgggtctg aactggaaac cgacgtttat cgacgaagtc aaaccggtga acgtggaagc
    17821 cgtgcgtgaa ctggccgctt ctggtgtgat gctgacgtcg gacgagaacg tagtgcgcgt
    17881 tgagatgaac gtgcagtacc gcgtcaccaa tccggaaaaa tatctgtata gcgtgaccag
    17941 cccggatgac agcctgcgtc aggctaccga cagcgccctg cgtggagtta tcggtaaata
    18001 caccatggac cgcattctga cggaaggtcg taccgtgatt cgtagcgata ctcagcgcga
    18061 actggaagag acgattcgtc cgtatgacat gggtatcacg ctgctggacg tcaacttcca
    18121 ggctgctcgt ccgccggaag aagtaaaagc ggcgtttgac gatgcgattg ccgcgcgtga
    18181 aaacgaacag caatacattc gtgaagcaga agcgtatacc aacgaagttc agccgcgtgc
    18241 gaacggtcag gcgcaacgta tcctcgaaga ggcgcgtgcg tacaaggccc agaccatcct
    18301 ggaagctcag ggtgaagtgg cgcgctttgc taaacttctg ccggaatata aagccgcgcc
    18361 ggaaattact cgcgagcgtc tgtatatcga gacgatggaa aaagtgttgg gtaacacccg
    18421 caaagtgctg gttaacgata aaggtggcaa cctgatggtt ctgccgttag accagatgct
    18481 gaaaggtggt aacgcccctg cggcgaagag cgataacggt gccagcaatc tgctgcgtct
    18541 gccgccagcc tcttcctcca caaccagtgg agcaagcaac acgtcgtcca ccagtcaggg
    18601 cgatattatg gaccaacgcc gcgccaacgc gcagcgtaac gactaccagc gtcaggggga
    18661 ataacgatgc gtaagtcagt tatcgcgatt atcatcatcg tgctggtagt gctttacatg
    18721 tctgtctttg tcgtcaaaga aggtgagcgc ggtattacgc tgcgttttgg taaggtactg
    18781 cgtgacgatg acaacaaacc tttggtttat gagcctggtc tgcatttcaa gataccgttc
    18841 attgaaacgg tgaaaatgct cgacgcacgt attcagacca tggacaacca ggccgaccgc
    18901 tttgtgacca aagagaagaa agacctgatc gtcgactctt acatcaaatg gcgcatcagc
    18961 gatttcagcc gttactacct ggcaacgggt ggtggcgaca tttcgcaagc ggaagtgctg
    19021 ttgaaacgta agttctctga ccgtctgcgt tctgaaattg gtcgcctgga cgtgaaagat
    19081 atcgtcaccg attcccgtgg tcgtctgacc ctcgaagtac gtgacgcgct gaactccggt
    19141 tctgcgggta cagaagatga agttactacc ccggcagcag ataacgccat tgccgaagcg
    19201 gcagagcgcg taacggctga gacgaagggc aaagttccgg tcatcaaccc gaacagtatg
    19261 gcggcgctgg gtattgaagt tgtcgatgtg cgtatcaagc agatcaacct gccgaccgaa
    19321 gtgtctgaag cgatctacaa ccgtatgcgc gccgagcgtg aagcggtagc gcgtcgtcac
    19381 cgttcacaag gtcaggaaga agcggaaaaa ctgcgcgcga ctgccgacta tgaagtgacc
    19441 agaacgctgg cagaagctga gcgtcagggc cgcattatgc gtggtgaagg cgatgccgaa
    19501 gcagccaaac tgtttgctga tgcattcagt aaagatccgg acttctacgc attcatccgt
    19561 agcctgcgtg cttatgagaa cagcttctct ggcaatcagg acgtgatggt catgagcccg
    19621 gatagcgatt tcttccgcta catgaagacg ccgacttccg caacgcgtta atataacgac
    19681 tgcggtacag gtcaataaag ccaccgcatc ctcagggatg tcggtggttt tctttttcta
    19741 taaggataat gaatgaattc gacaatctgg ctggcgcttg ccctggtttt ggtactggaa
    19801 ggtttagggc cgatgcttta cccgaaggca tggaagaaga tgatctctgc gatgaccaat
    19861 ttgcccgata atattttacg tcgttttggc ggtggacttg tggttgcggg cgttgtggtc
    19921 tactacatgt tgaggaaaac gattggctga acaaaaaaca gactgatcga ggtcattttt
    19981 gagtgcaaaa agtgctgtaa ctctgaaaaa gcgatggtag aatccatttt taagcaaacg
    20041 gtgattttga aaaatgggta acaacgtcgt cgtactgggc acccaatggg gtgacgaagg
    20101 taaaggtaag atcgtcgatc ttctgactga acgggctaaa tatgttgtac gctaccaggg
    20161 cggtcacaac gcaggccata ctctcgtaat caacggtgaa aaaaccgttc tccatcttat
    20221 tccatcaggt attctccgcg agaatgtaac cagcatcatc ggtaacggtg ttgtgctgtc
    20281 tccggccgcg ctgatgaaag agatgaaaga actggaagac cgtggcatcc ccgttcgtga
    20341 gcgtctgctg ctgtctgaag catgtccgct gatccttgat tatcacgttg ctctggataa
    20401 cgcgcgtgag aaagcgcgtg gcgcgaaagc gatcggcacc accggtcgtg gtatcgggcc
    20461 tgcttatgaa gataaagtgg cacgtcgcgg tctgcgtgtt ggcgaccttt tcgacaaaga
    20521 aaccttcgct gaaaaactga aagaagtgat ggaatatcac aacttccagt tggttaacta
    20581 ctacaaagct gaagcggttg attaccagaa agttctggat gatacgatgg ctgttgccga
    20641 catcctgact tctatggtgg ttgacgtttc tgacctgctc gaccaggcgc gtcagcgtgg
    20701 cgatttcgtc atgttcgaag gtgcgcaggg tacgctgctg gatatcgacc acggtactta
    20761 tccgtacgta acttcttcca acaccactgc tggtggcgtg gcgaccggtt ccggcctggg
    20821 cccgcgttat gttgattacg ttctgggtat cctcaaagct tactcaactc gtgtaggtgc
    20881 aggtcctttc ccggccgaac tgtttgatga aactggcgag ttcctctgca agcagggtaa
    20941 cgaattcggt gcaactacgg gtcgtcgtcg tcgtaccggc tggctggaca ccgttgccgt
    21001 tcgtcgtgcg gtacagctga actccctgtc tggcttctgc ctgactaaac tggacgttct
    21061 ggatggcctg aaagaggtta aactctgcgt ggcttaccgt atgccggatg gtcgcgaagt
    21121 gactaccact ccgctggcag ctgacgactg gaaaggtgta gagccgattt acgaaaccat
    21181 gccgggctgg tctgaatcca ctttcggcgt gaaagatcgt agcggcctgc cgcaggcggc
    21241 gctgaactac atcaagcgta ttgaagagct gaccggtgtg ccgatcgata tcatctctac
    21301 cggtccggat cgtactgaaa ccatgattct gcgcgacccg ttcgacgcgt aattctggta
    21361 cgcctggcag atattttgcc tgccgggcta acagtgtgat acattgctgt gtcggttaag
    21421 ccattacgct atccgacaca gtgttaaatc ctcgcttttt tccttccccg aactgaaata
    21481 aattagcgac acagcttgtg gctggtttat catcaatata aatgtatttt ttcccgattt
    21541 cccttttgag gttgatgtgc agttaacgag tttcactgat tacggattac gtgcgctgat
    21601 ctacatggcg tcattgccag aagggcggat gaccagtatt tctgaagtga ctgacgtcta
    21661 cggcgtctcc cgtaatcata tggtcaaaat aatcaatcaa cttagtcgtg ccggctacgt
    21721 gactgctgtt cgtggaaaaa atggcggcat tcgcctgggt aaaccggcga gtgcgatacg
    21781 tattggtgat gtggtgcgcg agctggagcc cttatcgctg gtgaattgca gcagtgagtt
    21841 ttgccacatt acacctgcct gtcggttgaa acaggcgctt tctaaggccg tgcaaagttt
    21901 tcttacggaa ctggataact acacgcttgc cgatttggtt gaagagaatc aaccgcttta
    21961 taaattattg ctggtggagt gacgaaaatc ttcatcagag atgacaacgg aggaaccgag
    22021 atgtcacaag atcctttcca ggaacgcgaa gctgaaaaat acgcgaatcc catccctagt
    22081 cgggaattta tcctcgaaca tttaaccaaa cgtgaaaaac cggccagccg tgatgagctg
    22141 gcggtagaac tgcacattga aggcgaagag cagcttgaag gcctgcgtcg ccgcctgcgc
    22201 gcgatggagc gcgatggtca actggtcttc actcgtcgtc agtgctatgc gctgccggaa
    22261 cgcctcgacc tggtgaaagg taccgttatt ggtcaccgtg atggctacgg ctttctgcgg
    22321 gttgaagggc gtaaagatga tttgtatctc tccagcgagc agatgaaaac ctgcattcat
    22381 ggcgatcagg tgctggcgca gccgctgggt gctgaccgta aaggtcgtcg tgaagcgcgt
    22441 attgtccgcg tactggtgcc aaaaaccagc cagattgttg gtcgctactt taccgaagcg
    22501 ggcgtcggct ttgtggttcc tgacgatagc cgtctgagct tcgatatctt aatcccgccc
    22561 gatcagatca tgggcgcgcg gatgggcttt gtggtcgtag tcgaactgac tcagcgtccg
    22621 actcgccgca ccaaagcggt gggtaaaatc gtcgaagtgc tgggcgacaa tatgggcacc
    22681 ggcatggcgg ttgatatcgc tctgcgtacc catgaaattc cgtatatctg gccgcaggct
    22741 gttgagcaac aggttgcggg gctgaaagaa gaagtgccgg aagaagcaaa agcgggccgt
    22801 gtcgatttgc gcgatttacc gctggtcacc attgatggcg aagacgcccg tgactttgac
    22861 gatgcagttt actgcgagaa aaaacgcggc ggcggctggc gtttatgggt cgcgattgcc
    22921 gacgtcagct actatgtgcg tccgccaacg ccgctggaca gagaagcgcg taaccgtggc
    22981 acgtcggtgt acttcccttc gcaggttatc ccgatgctgc cggaagtgct ctctaacggc
    23041 ctgtgttcgc tcaacccgca ggtagaccgc ctgtgtatgg tgtgcgagat gacggtttcg
    23101 tcgaaaggcc gcctgacggg ctacaaattc tacgaagcgg tgatgagctc tcacgcgcgt
    23161 ctgacctaca ccaaagtctg gcatattctg cagggcgatc aggatctgcg cgagcagtac
    23221 gccccgctgg ttaagcatct cgaagagttg cataacctct ataaagtgct ggataaagcc
    23281 cgtgaagaac gcggtgggat ctcatttgag agcgaagaag cgaagttcat tttcaacgct
    23341 gaacgccgta ttgaacgtat cgaacagacc cagcgtaacg acgcgcacaa attaattgaa
    23401 gagtgcatga ttctggcgaa tatctcggcg gcgcgtttcg ttgagaaagc gaaagaaccg
    23461 gcactgttcc gtattcacga caagccgagc accgaagcga ttacctcttt ccgttcagtg
    23521 ctggcggagc tggggctgga gctgccgggt ggtaacaagc cggaaccgcg tgactacgcg
    23581 gaactgctgg agtcggttgc cgaccgtcct gatgcagaaa tgctgcaaac catgctgcta
    23641 cgctcgatga aacaggcgat ttacgatcca gaaaaccgtg gtcacttcgg tctggcattg
    23701 cagtcctatg cgcactttac ttcgccgatt cgtcgttatc ctgacctgac gctgcaccgc
    23761 gccattaaat atctgctggc gaaagagcag gggcatcagg gcaacaccac tgaaaccggc
    23821 ggctaccatt attcgatgga agagatgttg caactgggtc agcactgttc gatggcggaa
    23881 cgtcgtgccg acgaagcaac gcgcgatgtc gctgactggc tgaagtgtga cttcatgctc
    23941 gaccaggtag gtaacgtctt taaaggcgta atttccagcg tcactggctt tggcttcttc
    24001 gtccgtctgg acgacttgtt cattgatggt ctggtccatg tctcttcgct ggacaatgac
    24061 tactatcgct ttgaccaggt agggcaacgc ctgatggggg aatccagcgg ccagacttat
    24121 cgcctgggcg atcgcgtgga agttcgcgtc gaagcggtta atatggacga gcgcaaaatc
    24181 gactttagtc tgatctccag tgaacgcgca ccgcgcaacg tcggtaaaac ggcgcgcgag
    24241 aaagcgaaaa aaggcgatgc aggcaaaaaa ggcggcaagc gtcgtcaggt cggtaaaaag
    24301 gtaaactttg agccagacag cgccttccgc ggtgagaaaa aaacgaagcc gaaagcggcg
    24361 aagaaagacg cgagaaaagc gaaaaagcca tcggcgaaaa cgcagaaaat agccgcagcg
    24421 accaaagcga agcgtgcggc gaagaaaaaa gtggcagagt gatcaatacc ctctttaaaa
    24481 aaagagggtt agattgctga caaaatgcgc tttgttcatg ccggatgcgg cgtgaacgcc
    24541 ttatccggcc tacataatca cgcaaattca atatattgca gagatcatgt aggccggata
    24601 aacgtagtgc atcaggcaaa acgtaaacaa cgagtacatt aatgagcgaa atgatttacg
    24661 gcatccacgc agtgcaggcc ctgctggagc gcgcccctga acgttttcag gaagtcttta
    24721 ttttaaaagg ccgtgaagat aaacgtctgt taccgctgat tcacgccctt gagtctcagg
    24781 gcgtggttat ccagttggca aaccgccaat atctcgacga gaaaagcgac ggtgccgtgc
    24841 atcagggcat tatcgcccgc gtgaagccag gacgtcagta tcaggaaaac gatctgccgg
    24901 atctgatcgc ttcgctcgat caaccgttcc tgctgatcct cgacggcgta accgatccgc
    24961 acaacctcgg cgcgtgcctg cgtagtgcgg acgccgcagg cgttcatgcg gtgattgtgc
    25021 cgaaagatcg ctccgcacag ctcaacgcta cggcgaaaaa agtagcctgc ggcgcggcag
    25081 aaagcgttcc gctgattcgg gtgactaacc ttgcgcgcac catgcgtatg ttgcaggaag
    25141 agaatatctg gatcgtcggt acggcaggcg aggcggatca tacactctat cagagcaaaa
    25201 tgaccggacg cctggcgctg gtgatgggcg cggaaggtga aggtatgcgt cgcctgaccc
    25261 gtgaacattg cgatgagttg atcagcatcc caatggcagg aagtgtttct tccctgaacg
    25321 tttcggttgc aaccggaatt tgcttatttg aagcggttcg tcagcgcagc tagttcctct
    25381 gaattatgaa aagccatcca gatttggatg gtttttttgg taatgctgcc aacttactga
    25441 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt ccatttccat cagatgtcct
    25501 tcctgctccg ctactgaagg cgtggtgcgt aacgg
//
LOCUS       DAECNO010000057        24893 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.057, whole genome shotgun sequence.
ACCESSION   DAECNO010000057 DAECNO010000000
VERSION     DAECNO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.057"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            167..1666
                     /gene="pitB"
                     /locus_tag="KKO08_002387"
     CDS             167..1666
                     /gene="pitB"
                     /locus_tag="KKO08_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBD6318607.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1715..2407)
                     /locus_tag="KKO08_002388"
     CDS             complement(1715..2407)
                     /locus_tag="KKO08_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD6318608.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2581..3294
                     /locus_tag="KKO08_002389"
     CDS             2581..3294
                     /locus_tag="KKO08_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBD6318609.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3326..4084
                     /locus_tag="KKO08_002390"
     CDS             3326..4084
                     /locus_tag="KKO08_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318610.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4130..5479
                     /locus_tag="KKO08_002391"
     CDS             4130..5479
                     /locus_tag="KKO08_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBD6318611.1"
                     /translation="MAGFNIKHWFADGAFRTMIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5479..6303
                     /locus_tag="KKO08_002392"
     CDS             5479..6303
                     /locus_tag="KKO08_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318612.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6315..6875
                     /locus_tag="KKO08_002393"
     CDS             6315..6875
                     /locus_tag="KKO08_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBD6318613.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6906..7976
                     /gene="lptF"
                     /locus_tag="KKO08_002394"
     CDS             6906..7976
                     /gene="lptF"
                     /locus_tag="KKO08_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD6318614.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7973..9052
                     /gene="lptG"
                     /locus_tag="KKO08_002395"
     CDS             7973..9052
                     /gene="lptG"
                     /locus_tag="KKO08_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD6318615.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9088..10259)
                     /locus_tag="KKO08_002396"
                     /pseudo
     CDS             complement(9088..10259)
                     /locus_tag="KKO08_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10259..10507)
                     /locus_tag="KKO08_002397"
     CDS             complement(10259..10507)
                     /locus_tag="KKO08_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBD6318616.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10540..11454)
                     /locus_tag="KKO08_002398"
     CDS             complement(10540..11454)
                     /locus_tag="KKO08_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBD6318617.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11451..13181)
                     /locus_tag="KKO08_002399"
     CDS             complement(11451..13181)
                     /locus_tag="KKO08_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBD6318618.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13543..14684
                     /locus_tag="KKO08_002400"
                     /pseudo
     CDS             13543..14684
                     /locus_tag="KKO08_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(14808..15578)
                     /gene="glcC"
                     /locus_tag="KKO08_002401"
     CDS             complement(14808..15578)
                     /gene="glcC"
                     /locus_tag="KKO08_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBD6318619.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            15834..17333
                     /gene="glcD"
                     /locus_tag="KKO08_002402"
                     /pseudo
     CDS             15834..17333
                     /gene="glcD"
                     /locus_tag="KKO08_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            17333..18385
                     /gene="glcE"
                     /locus_tag="KKO08_002403"
     CDS             17333..18385
                     /gene="glcE"
                     /locus_tag="KKO08_002403"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBD6318620.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            18396..19615
                     /gene="glcF"
                     /locus_tag="KKO08_002404"
                     /pseudo
     CDS             18396..19615
                     /gene="glcF"
                     /locus_tag="KKO08_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            19620..20024
                     /locus_tag="KKO08_002405"
     CDS             19620..20024
                     /locus_tag="KKO08_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBD6318621.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            20046..22217
                     /gene="glcB"
                     /locus_tag="KKO08_002406"
     CDS             20046..22217
                     /gene="glcB"
                     /locus_tag="KKO08_002406"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBD6318622.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            22572..24167
                     /locus_tag="KKO08_002407"
     CDS             22572..24167
                     /locus_tag="KKO08_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HBD6318623.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         5963 a         6070 c         6628 g         6232 t
ORIGIN      
        1 acctgtgttt tgattatttc taaaggtttt gaatgaatgc ttattgtctg atacacaaga
       61 aataacactc tttttatcgt taaaaaatga tatttcactt tgcccatgcc gttaaagtca
      121 ctgataatgc gtccgttcgt aaattcaaaa tggcgtaatc taatatatgc taaatttatt
      181 tgttggcctt gatatatata cggggctttt gttattactt gctctggcat ttgtgttgtt
      241 ctacgaagca atcaatggtt ttcatgacac ggcgaatgcg gtggcaaccg ttatttatac
      301 tcgtgccatg cagccacaac ttgctgtggt gatggcggca ttttttaact tttttggcgt
      361 gttattgggc ggacttagcg ttgcctatgc cattgtccat atgttaccaa tcgatttgtt
      421 gctgaatatg gggtcaactc acggtttggc gatggtcttt tccatgctgc tggcggcgat
      481 tatctggaac ctgggaacgt ggttctttgg tttaccggcc tccagttcgc acaccttgat
      541 tggtgcgatt atcggcatcg gtttaaccaa cgcgctgtta accggctcat cggtgatgga
      601 tgcgttaaac ctgcgtgaag taaccaaaat tttctcctcg ctgattgttt cccctatcgt
      661 cggcctggtc attgcgggtg gtctgatttt cctgctgcgc cgtttctgga gtgggacgaa
      721 aaagcgtgac cgtattcacc gcattccgga agatcgcaaa aagaaaaagg gcaaacgtaa
      781 accgccattc tggacgcgta ttgcgctgat tgtttccgct gcgggcgtgg cgttttcgca
      841 cggcgcgaac gacgggcaga aagggatcgg cctggtaatg ctggtactgg tggggattgc
      901 ccctgctggc ttcgtcgtca atatgaatgc gtccggctat gaaattaccc gtacccgcga
      961 tgccgttacc aacttcgaac actacctgca acagcatcct gaactgccgc agaagttgat
     1021 tgcgatggaa cctccattgc ctgcagcatc gactgatggc acgcaagtaa cagagtttca
     1081 ctgtcatccg gcaaatacct ttgatgcgat tgcgcgcgtt aaaacgatgc tgccaggcaa
     1141 tatggaaagt tacgagccgt taagcgtgag tcagcgcagc cagctgcgcc gcattatgct
     1201 gtgcatctcc gatacctccg cgaagctggc gaaactgcca ggcgtcagta aagaagacca
     1261 gaacctgctg aaaaaactgc gcagcgatat gttaagcacc attgagtacg ctccggtgtg
     1321 gatcatcatg gcggtagcac tggcgctcgg cattggcacc atgattggct ggcgtcgtgt
     1381 agcgatgacc atcggtgaga agattggtaa gcgcggcatg acgtatgcgc aaggcatggc
     1441 ggcacaaatg acggcggcag tgtctatcgg ccttgccagt tatattggga tgcctgtctc
     1501 cacaacacac gtcctctcgt ctgcggtagc agggacgatg gtggtggacg gcggtgggtt
     1561 acagcgtaaa acggtaacca gcatcctgat ggcgtgggtg tttactttac cggcggcaat
     1621 ttttctttct ggtgggctgt actggatagc attgcagttg atttaacctt cctgaaaatg
     1681 cccggtcctg gcgatcgggc atttccattt ttgactagtg ataaccacgc gcggtcataa
     1741 aatccgtaat cgctttttct gcatcaacca acacctgttc cagtggctga ttggcatcga
     1801 tatcaaccag ttgtgcacca ccaaaggtta actgtggcgt tatggcaatc ttcctcgcca
     1861 gcgattcccg tttatggtcg ggtttacgtg cacaggcaac ttcaaggtca acattgagtt
     1921 tgatgaccag atcaggctta tggctcgcca tccagtgaaa cgctttacgt tcctggcttg
     1981 ccagccatga gacaaaacga ccaccttcaa cgttaggtgg gaacaccgta ccatcgtaag
     2041 cgccaggaat ttggtcctga ggataacggt cggttagaac aattaacccg cgacgacgac
     2101 aggcaagcat atgacgaaag cgcagtaagc gacgggcgac aaacgctgta attaccagcg
     2161 ccggaactgg tccaggcaat ttttttgctg ttttcacctg atttcgttca attgttttat
     2221 gtaaggattt tcccatcaaa ggtaattttg tcactgcacg accgacattt ccggcctgtt
     2281 ttcctaaatg aactctttcg gcagcaccat atttttcgac aacggtaata agatgttcac
     2341 acaccgttga cttgcctgaa ccatcgctac caataacggc aattaatgga ggtgttattg
     2401 actgcatgaa tatatccttt aatttaaata tccattaaaa atatttattt ggttaatatg
     2461 tttttatgaa agcgtaattc aggtcaatgt cacaattagc cattgtcaca ataaggttga
     2521 acggatattc tgagcacgga ccacgtaata ttcaatacat tatttactgc cgggtttttc
     2581 gtgaatcaac gtaatatgtc aataattaat tccacgccag tgcgtgttat tgccattgta
     2641 ggatgtgatg gttcaggtaa atcgaccctc acggcaagcc tggtaaatga actggcagca
     2701 agaatgccaa cagaacacat ttatctcggg caatcgtccg ggcgaattgg cgaatggatt
     2761 tcacagctcc ctgttattgg cgcaccttgt gggtgttatc tgcgaagtaa agcggcacat
     2821 gtacacgaaa agcccttaac tccgcctggc aatattactg cgctggttat ctatctgctt
     2881 tcctgctggc gggcgtacaa gtttcgcaaa atgttgtgta aaagccagca aggctatctg
     2941 ctcatcaccg accgctaccc gcaggttgaa gtgccgggtt ttcgctttga tggcccgcaa
     3001 ttggcaaaaa ccacgggcgg taacggttgg ataaaaatgt taaggcagcg cgagttgaag
     3061 ctgtaccaat ggatggcgtc ttatttaccc gtattgttga ttcgtcttgg cattgatgaa
     3121 caaaccgcgt ttgcgcgtaa acctgaccac caactggcag cgttacagga aaaaatcgcc
     3181 gttacgccgc aactgacatt caatggcgcc aagatccttg aactggatgg tcggcaacca
     3241 gccgatgaaa ttatgcaagc gtcactacgc gcaattcacg ccgcgctttc ctgatcacca
     3301 aaatggatag gggatgattt aacgaatgga tgcactacaa acacaaactg ttaatagtac
     3361 aaccgcgccg cagcccaact acattccggg gctgattgcg gtggtcgggt gtgatggcac
     3421 cggtaaatcc acgctgacca ccgacctggt gaaatcgctg caacaacact ggcaaaccga
     3481 gcgccgctat ctggggctgc tctccggcga agacggcgac aaaatcaaac gattgccgtt
     3541 ggttggcgtc tggctggaac ggcgattggc ggccaaatcc tcgaaaaccc aaagcatgaa
     3601 aaccaaatct ccggcgctat gggcggcggt gattatgtac tgcttctcgc tgcgaagaat
     3661 ggcgaatcta cgcaaggttc agcgactggc gcaaagtggc gttctggtgg tcagcgatcg
     3721 cttcccgcag gctgaaattt cgggctttta ttatgacgga ccggggatcg gcgtcgaacg
     3781 tgcgaccggg aaaatcagca tgtttctggc gcagcgcgaa cggagtttat accaaaaaat
     3841 ggcgcaatac cgtcctgaat taatcattcg tttggggatt gatattgata ctgccatctc
     3901 ccgcaagcct gaccatgagt atgccgagct gcaggacaaa atcggcgtca tgtcgacgat
     3961 tggctataac ggcacaaaaa ttcttgagat tgattccaga gcgccctaca gcgaagtgct
     4021 ggaacaggta cagaaggcgg tttctctggt tgccatcgtt tctgaccgcc gaagtttaac
     4081 ttaaggctgg cgggatattt tttactatca acgataaggt caggctgaat tggcgggttt
     4141 taacatcaaa cattggtttg cagatggcgc gtttcgcacc atgattcgca atagcgcctg
     4201 gttaggctcc agtaatgtcg agagcgcctt gctggggctg ttggcgctct cgtgtgccgg
     4261 taaagggatg acgcccgcca tgtttggtgt actggtgatt gtgcaatcgt acgccaagtc
     4321 gatcagcgat tttattaagt ttcagacatg gcagctggtg gttcagtacg gaacgccagc
     4381 cttgaccaac aataatccgc agcaatttcg caatgtcgtc tcattttcct tctcgctgga
     4441 tatcgtcagc ggcgcggtgg cgattgtcgg cggcattgcc ttactgccat tcctttccca
     4501 ttcattaggt ctggatgacc aaagcttttg gctggcggcg ctgtattgca cgctcattcc
     4561 ttcaatggct tcctccacgc cgaccggcat tctgcgtgcg gtagatcgct ttgatttgat
     4621 tgctgtacag caggcgacga aaccttttct gcgcgcagcg gggagcgtcg cagcctggta
     4681 ttttgacttt ggttttgcgg gttttgttat tgcctggtac gtgtcgaatc tggttggcgg
     4741 caccatgtac tggtggtttg ccgcgcgcga attacgccgt agaaatatcc ataacgcctt
     4801 caaattgaat ctgtttgagt ctgcccgaca cattaaaggc gcgtggagtt ttgtctggtc
     4861 aaccaacatt gcccactcca tctggtcggc gcgtaactcg tgcagcacag tgttggtggg
     4921 gatcgtgtta ggacccgctg ccgccgggtt atttaaaatc gccatgacat tcttcgacgc
     4981 cgccggaacg ccagcgggtt tgctgggtaa aagtttttac ccagaggtga tgcgtttaga
     5041 tccgcgcacc accagaccgt ggttgttagg tgtgaagtcc ggtttactgg cgggcggaat
     5101 cggtattctg gtggctctcg cggtgttgat cgtcggcaag ccgctcattt cgctggtgtt
     5161 tggcgttaag tatctcgaag cgtatgacct gattcaggtg atgctgggcg caattgtgat
     5221 ttcgatgctg ggcttcccgc aggagtcatt gctattgatg gcgggaaaac agcgcgcttt
     5281 tctcgtggcg caaaccatcg cctcaatcgg ctacatcgta ttgctgttca tgttctgtca
     5341 tctgtttggt gtgctgggag cagcgtttgc ttacttcggt gggcaatgtc tggacgtggc
     5401 gctctcgtta attccaacct taaaggcatt ttttcagcgc cattccttgc tttataacgc
     5461 agccggagag aaatcctgat gatgagaaaa tatttcccgc ttgaggcgag cgagcgtcta
     5521 tttgttgcga ttgaagaaga tgatgtcgtt gatgcacagg ttagtttgcc gccgactatt
     5581 gcactaagtt gtaccaccga aatcattcat gataattacg cgttatgcct gcaattctgg
     5641 ctcaatggcg ttgatcgtca ggaattactg cgtttagttc gcaaacaagc caaaggcgat
     5701 gagctgactg ctgatgagcg caaacaattt aaatacatgc gagcgcgcta caagcacctg
     5761 cgttttgcgc aacggctgta tttaaagaag catcaggccg gatttttatt cggcaaaacg
     5821 accgtttttc tggggcgttt tcaggacggc tttcgtaacg gtaagaaaaa tattgtgtcg
     5881 tattacggca acctgttacg gatttattta agctctccgg tctggtcgtt ggtgaactat
     5941 tcattacgcc atagccagtt agaaagcgta agcagtttta ttgcctatcg gcaaaagcaa
     6001 atgcataccc tgaaagagat tattgccaaa ccgcgactaa ccggcaggga atttcatgat
     6061 gtgcgaaaaa tcatcagcca gcaggtttct tattacgaca ccttaagatc gcttgatcct
     6121 gaaaataaag aggcgttaca gatttcccgt ttcctcgccg ccattaatgg cctgatggga
     6181 gataaacacg acgacatggt ggcggatgat atggaaaacc gccagtcgta ttatgcaccg
     6241 ttggcgctgg atagcgatat ttgtcagcgc ttagagctgc tgatttcgcg ttttccgcta
     6301 tagccggagc aatgatgtca tctcgtttga ttattgcgtt gattatcatg ctcttagccc
     6361 cgggtgtgca ggcacacaat ttcgtcactg gcaaaacggt tacccctgtt tatatccagg
     6421 aaggcggcga gctgcttctg aatagcgacg acgaaattca ctaccaaaag tggaacagca
     6481 cacaattagc gggaaaagtc cgcattattc agtatatcgc cgggcgtaag tcggcgaaga
     6541 aaaagaactc actgttaatt aaagcggttg aagcggcaaa ctttccgcag gatcgttttc
     6601 agcccaccac cattgtgaac accgatgatg ccatctttgg tactggctat tttgttgttg
     6661 gcaaaatcga aaaaaataaa cgccgctatc cgtgggcgca attcgttatt gacggcaatg
     6721 gacagggacg cgttgcctgg cgcttgccgg agcagagttc aaccattctg gtgctgaata
     6781 aagccgggca aattcagtgg gcaaaggacg gcagcctgac gccggaagag gtagatcatg
     6841 tcattgcgtt ggcgcagaaa ctgatcaatg aataaaaaac tcaaaggatt gagggcgtta
     6901 gtgagatgaa gctggttgaa cactacatca tgcgtggtac gcgccgtctg gtgctgatta
     6961 tcgtcggttt tctgatcttt attttcgcca gctactccgc acagcgttat ctgaccgaag
     7021 cggcaaatgg gacattagct cttgatgttg tgctggatat cgttttctac aaagtactga
     7081 ttgcactgga gatgttgtta cctgttggtc tgtatgtgtc agttggcgtg acgctagggc
     7141 agatgtatac cgactcggaa attaccgcta tctctgcggc ggggggcagt ccgggacgct
     7201 tgtacaaagc cgttctttat ctggcgatac cgctaagtat ttttgtcacc cttctgtcga
     7261 tgtatggtcg tccgtgggct tatgcgcaga tttatcaact ggagcagcag tcgcagtcgg
     7321 agctggatgt tcgccagttg cgggcaaaga aatttaacac taacgataac ggacgaatga
     7381 tcctttcgca gacggttgat caggataata atcgcctgac tgacgcgctg atttatactt
     7441 ctactgccaa tcgaacccgt attttccgcg ccggttcggt tgatgtggtt gacccatcac
     7501 ccgagaaacc gaccgttatg ttgcacaacg gcactgccta tcttctcgat catcaggggc
     7561 gtgacgacaa cgaacagatc taccgtaatc tgcaattaca tctgaatccg ctggatcaaa
     7621 gcccgaacgt caaacgcaaa gcgaaatcgg tcacggagct ggcgcgctcc gcctttcctg
     7681 ccgatcatgc cgaactgcaa tggcgacaaa gccgtggcct gaccgcattg ttgatggcgc
     7741 tgctggccat ttcattaagt cgggtaaaac cgcggcaagg gcgattttca acgttattgc
     7801 cactgacgtt gctgtttatt gccatttttt atggcggcga cgtctgccgt acactggtgg
     7861 ctaacggcgc gattcccctc attcctggtt tgtggttagt gcccggactc atgctaatgg
     7921 gcctgctgat actggtcgca cgtgacttct ctttgctgca gaaattttcc cgatgaatgt
     7981 tttcagtcgc tatttaatcc gtcatctctt tctcggtttt gccgccgctg cagggctatt
     8041 gctgccgctt tttaccacct tcaacctgat taacgaactg gatggtgtca gcccgggcgg
     8101 ttatcgctgg actcaggcgg tgctggtggt gctaatgacc ttaccgcgca cactggtcga
     8161 actttcgccg tttatcgcgt tattgggtgg tattgttgga ctggggcagt tatcgaaaaa
     8221 cagtgagctt accgccattc gcagcacggg gttttctatc ttccgtattg cactggtggc
     8281 gctggttgcc gggatattgt ggactgtttc gttaggcgcg atagatgagt gggtggcgtc
     8341 gccattgcag caacaggcgt tgcaaatcaa atcgaccgcc accgcgttgg gggaggacga
     8401 tgacattacc ggcaatatgc tgtgggccag gcgtggtaat gaatttgtga cggtgaaatc
     8461 gctgaacgag cagggccagc cggtgggcgt ggagatattt cattatcgcg acgatctctc
     8521 gcttgaatcc tacatttatg cacgcagtgc caccattgaa gacgacaaaa cgtgggtcct
     8581 gcatggtgtg aatcataaaa aatggctgaa tggcaaagaa acgctggaaa cattagataa
     8641 tcttgcctgg caatcggcct tcaccagtat gaatcttgaa gagttatcaa tgccggggaa
     8701 tactttttct gtccgtcagc ttaatcatta catccattat ttgcaggaaa ccgggcaacc
     8761 cagcagcgaa taccgccttg cactgtggga aaaactgggg caacccatcc tgaccctggc
     8821 gatgattttg ctggctgtgc cgtttacctt tagtgccccg cgttcgcctg ggatgggtag
     8881 ccgtctcgct gtaggcgtca tcgtgggctt actcacctgg atcagctatc aaatcatggt
     8941 taatctggga ttgctatttg cgttaagcgc acctgttacc gcgctcggtt taccggtagc
     9001 gtttgtgttg gtggcgttga gcctggtgta ttggtatgac agacaacatt aaacccgccg
     9061 tggcgcaaac accacggcgg gctcacatta ctgcttcaga gtagcaaatg cctgaatgat
     9121 ggcatcgatt tgctcacgac tgtgggcagc atttacgcta cagcgcagca gcgtgatgcc
     9181 tgcaggcgcg gcaggcggca ggataagatt cacatacacg cccggcgaga taagctcgcg
     9241 ccagatgcgt aaaccttcct ctttcgaacc gattattact ggcactaccg ggctaatatg
     9301 tgaacttaac tcataaccca gtttttgcag accgtcatac agatggttgg cgttatccat
     9361 cagcttttgc cgcagctctg gttgtgtggc aatagttctt aacgacgagc gcacggtcgc
     9421 aatgcaggac ggagaaggag aggcggtaaa aatatacggg cggctgccgt agcgcagcac
     9481 ttccatcgct tccgaaccga cagcaaaacc gccaatcgag gccagacttt tactgaaagt
     9541 accaacgata atatccacat catcttcaac gccgacagct tcagccagtc cgcgccccgt
     9601 tgcgcctaac acgccaaacg aatgcgcttc atcgacaatc aaataaccac cgagacgacg
     9661 tttgatatca acaatttccg ccagcggcgc aacgtcaccg agcatgctgt agatgccctc
     9721 aacgatgata atcgcctctt tcgcccgttc accgaggcga accatgcgcc gttccagatc
     9781 tttagcatcg ttatggcgga aacgaataat ttccgcgcca ccgagagaac aggcgtcata
     9841 aatactggcg tggctgtcgg catccagtaa caccacggcg ttatggtcgg caagtgcgct
     9901 gatgacgcct aaattagcgg tataaccggt tgaaaaacga tagcggtagg gagattgaaa
     9961 aactcggcaa tctctttctc taacgccagg tgcggcgcat aactgccgtt tgccatacga
    10021 gaaccggtgg tgccggtgcc ttgggccgct aacgcggcct gaccttcgct gatggcatca
    10081 tgattaaagg ttaaaccgag ataattattg gtcccggcca gaatcacttt tttattgccg
    10141 atccggcctt cggtggcgga atacacttca tcaatacagg tgccaaacgg tgttaacccg
    10201 ttgtcactaa attgcagacg ctcgccagca aggcgcgcat atttatcgta tagccccatt
    10261 atttattctc cagccatgga agtagagttt ccattaattg cgcaggggtt ttgacatcca
    10321 gcagaatatt aataggaatg gaaatatcga atctgtcttc cagcatcatt aagagatcca
    10381 tcactttgat cgactccagg ccaagatcgt tgacgagatc gctgtcgggc ttaatttcca
    10441 caccgttttc gaccaaatcc tgtaaacagg aaaggatgta atccattact atttcacgat
    10501 ttaccataga aacgttaaca cacctttaaa acaagacaat caaaatagcc cgttgcgtaa
    10561 tgcgtgttcc aggctaatcc cgggaaacca attgatatct tcagaaatac ggttattact
    10621 tgccgaccag tcggcgtggg ttaattcacg aattttcgag cgggtcagca taggttcttt
    10681 acctgccagg cgactcaacg cggtactgat atccgcaagg cctgtgagta ccggcagagg
    10741 aatgtcaacc attcgcacgg aaccacaacg ggctttggcg gcaagctgtc gtacgcgttg
    10801 ccagtcatag ccaccggcga cgccatcgca taattcatag gtttgcgtct gtacagtttc
    10861 ggcgcttaac cactgaccca ctgcttgcgc aaaatcggtg acgtgaagaa aagagagttg
    10921 tgtatctggc gcaccaagtc gtggcagcag gccgcgtagc atccagtcaa acagcggttt
    10981 taactcttta tcgccgggac catacacggc tgtcgggcga aaaacgccca gcgtaatttc
    11041 gtccgccatt gctgccagtc gttgctcggc gacgtatttg gattttgcat accaggagag
    11101 ctcgggatgg cgcgccgcca gcgaagagat aaacagaaaa cgttggcaaa agccgctctc
    11161 ttttgctgct tgcatcaggc gcaggctgcc gtcaacgtta cagcgggtga aaatctcttc
    11221 tttgtgcccg cgcacttgcc cggcgcaatg gaccactacg ctggctccgg caaccagctc
    11281 gctaagtgaa tgagtatctt ccagcgaacc gcgcacccag gtgagattat cattgacgtg
    11341 agcgcgggca gtacgcgtca atgcgcgaac gtgaaagccg cgggcgagca ggttatcgat
    11401 aatatattta ccgataaacc cggtagcgcc cgtcaccgcg acagtttgat ttatgccagg
    11461 gattcctgca cattaagact ggcagcataa gccttctgat aacgtttttt cgcttccgca
    11521 cgggcaggct tgccggagga cgttcgggga atactgtggg gcggcaacag atcgatagcc
    11581 gcggtcacgc caaattcgct ttggatccgt gccgccagcg cgtggataag ctgcccgcga
    11641 cgttcttcgt cgctgatccg acactggatc tgcaaaatga ttttttcctg ggcggtaaca
    11701 aaagcaatcg catcgccaga atgaatttcc ggttcctgtt ccgctatata ttcaatatcc
    11761 tgcggccaga tattacggcc acgaataata atcagatctt taatgcgtcc ggtgacatac
    11821 agataaccgt ccagcagata accgaggtcg ccggtgtcta accagcccgt cgcggcaatc
    11881 tcgtcttgcg aaacctggtc gccaaagtaa ccgctcatca gactgggacc ggagatgcaa
    11941 atatggccta ccacacgttc cgcgaccggc atacctgctt cattgcggat ttcaatacca
    12001 tgttccggca acgctttgcc gcagttgacg aaagtcgata cggcgcgtgt ctctgcaccc
    12061 ggcgcgacgg ctttaccctg atattcgagg atgtcgcgat ccacttcgtt aaccacaacc
    12121 ccggaggctt catcagagaa gctgacagcc agcgcatttt ctgccagtcc gtagcacggc
    12181 atgaaagttt tattgtcaaa gttaacctga cggaaacatt cagcgaattg atggagttgt
    12241 tctgcggaga tcggctcagc accaatacca gcgacgcgcc agcaggaaag atccagttca
    12301 gcgagatctt tttcattcac gcggcgttgg cacaattcat agccaaacgg cggcgcaacg
    12361 gaaacggtgc cgcgattttt actgatcaat ttaagccatt gcaaaggacg catggcaaaa
    12421 tcctgagtgc gcaaataatc tactgaaagt tgcgtggcga cggggggcag gagaaagccg
    12481 accagtccca tatcatggta gaaaggctgc caggagacgc agcggtcgct agggcgtaat
    12541 ttaatgccat cgtggcttat agcacgtaga ttagccatca cttcgcgatg ggtgataatg
    12601 acgccacggg gaaaacgggt gctgccggag gtgtactgga ggtaggcgat atcgttcgga
    12661 actggacgct ggagcgcaac atcggcttcc ggtaatgcct taaaccaagc gtggcttaaa
    12721 acatgtaatt cggggttgtt atgcgtcgcg gcattgacca gtggcaacca ctcatcacca
    12781 gtgataatgg ctgcgggctg gcaacttgcc agtaaaccct ggagtttggc gctccaggaa
    12841 tcccgctgac cgacgcccat tggaatcgcc aacgggacgg cgactaagcc agcatactga
    12901 caggcaaaaa aagcctctac aaacccgcta ctcgtttcgg caatcagcgc cacgcgttcg
    12961 ccttttttca ggttcagcga taacaaccgc ttcgcaccag cttcggcacg cgtttttaat
    13021 gtctgatatt ccagttgatc ttcaagttgg caacgtctgt cataaaaatt cattccggct
    13081 ctactcagag cggcgtagtc caaagcatca accagcgttg gaaaatcggc atagcgcata
    13141 ggtagggaat gcgtaaagat tttattagac atatacacca cgaaaaaatt ggatatcaaa
    13201 aataaaatga caataatgcg gtggattata taggcataaa agataaatga gatagtattt
    13261 tcatcggtgt gaaaatgaac attgatttaa ataatgagtt taagtttttt gtttttatta
    13321 tgaatgatat ttaatttatg aatatattaa gaagaagcct tgtccggtcg ccttaaattg
    13381 acaaaaggca ttgaggcgtt tctgtgtagc ctctgacctt atcataatca ttaattaata
    13441 ctatttattc gtcgtttgta ttcattgagt tgattattaa gatgccgctc gttgctgttg
    13501 caacctgttt ttcaccatta attatttgtt atccacagag atatggaatg cgatctgctg
    13561 atgattaaaa ttgaaaaagt aattaataaa aatgacctta aggcatttat cgcttttccg
    13621 tcatcacttt atcccgacga tccaaactgg ataccccctt tattcattga acgcaacgag
    13681 catttgtctg cgaaaaatcc ggggacggac catatcatct ggcaggcgtg ggtggcaaaa
    13741 aaagcggggc agatagttgg gcgtattacc gcgcaaatag ataccttgca ccgtgagcgt
    13801 tacggtgaag ataccggtca tttcggcatg attgacgcca ttgatgatcc gcaggatttt
    13861 gccgcgttgt ttggcgcagc ggaagcgtgg ttgaagtcac aaggtgcaag taagatcagc
    13921 gggcctttca gcctgaatat caatcaggaa agcggattac tgattgaagg ttttgacaca
    13981 ccaccctgtg cgatgatgcc acacggcaaa ccgtggtatg ccgcgcatat tgaacaactg
    14041 ggttatcaca aaggcattga tttactggcg tggtggatgc agcgaaccga tctcactttc
    14101 tctccggcgc taaaaaaact gatggatcag gtgcgcaaaa aggtgaccat tcgctgcatc
    14161 aatcgtcagc ggtttgccga agagatgcag atcctgcgtg agattttcaa ctctggctgg
    14221 cagcacaact ggggatttgt gccgtttacc gaacatgaat tcgcgaccat gggcgaccaa
    14281 cttaaatatc tggtgccgga cgatatgatc tacatcgctg agattgattc tgcaccctgc
    14341 gcgtttattg tggcttgcct aacatcaacg aggcgattgc cgatctgaac ggatcgctct
    14401 ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc
    14461 gagtaccgct gatgggcgtg cgcgacgagt atcagttcag ccgcatcggc ccggtgattg
    14521 cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg
    14581 agatgtcatg gatcctcgaa accaatacag gcatgaataa catgctggaa cgcattgggg
    14641 cggagccgta caagcgttac cgattgtatg aaaagcaaat ttaataccag atcctgttta
    14701 gcgcaggggc ggtcattcgg gagagggtat agatgaacag atttaacacc agatcctgtt
    14761 aagtgcaggg gcggtcccct cgccttgggg atagggtaaa actctgctca cattcccacc
    14821 ttgttcatct ccagcggcac cgccgagcga atcaaatggt ggccttccag ctcgatatcg
    14881 tggagattct ttttcacggt ccgtacatga tcgcgcgctg cgcgctgagc gacgtgcggc
    14941 agccgctgca acaccgcgtt gtagatccgc gcatgctggc gatcgatctg ctttttttgc
    15001 tgtggtcgat ggtagagatt atttacgctg gcaaacactg aattaaacat cagatcggtc
    15061 aatgattgca gcgtaaacac cagcacctga ttgtgagaag cctgacaaat ggcgagatgg
    15121 aaagcgtgat ccaactgcgc atgttcgatc agcgaaatct ctttgtggtt ctcactggcg
    15181 gcgagcattt tttcataaca gcgggttatc acaacaaaat cagcctgcgt tcccagcgtt
    15241 gccgccagcc ttgccgattc gccctccagt aatgcgcgaa cgtcgagcag atcgtacagc
    15301 gttcgcggct gcgtactgaa cagatggatc agcgggctgg tgtcctgctc ccgattaagc
    15361 cgtgcgacat gagaatcgcg accctgcgcc gtttcaataa tcccgcgccc gcgcagcacg
    15421 gtcagccctt cacgcagtgc ggagcgtgag aagccgagct tttcacacag tcgacgttcc
    15481 gagggaagcg gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc gatactctct
    15541 gcaaccactt cgcaaatagg gcgacgttca tctttcattc ccggacctcg tgcacaggta
    15601 ggaccaattt ttctgttctt aactcgcaaa acacgcacat cacgcaagtg attatgttac
    15661 atcaatttaa cattaagtta accaagacaa ggtcacagag ctggaaaaaa aatggtctga
    15721 ccggtagcta aagagataga cgaaaacgaa aagcccgctt aataactgtt cacagaagca
    15781 gcgcgcaaaa atcagctgcc acacaacaca acacaacaaa gcgaagccta ctcatgagca
    15841 tcttgtacga agagcgtctt gatggcgctt tacccgatgt cgaccgcaca tcggtactga
    15901 tggcactgcg tgagcatgtc cctggacttg agatcctgca taccgatgag gagatcattc
    15961 cttacgagtg tgacgggttg agcgcgtatc gcacgcgtcc attactggtt gttctgccta
    16021 agcaaatgga acaggtgaca gcgattctgg ctgtctgcca tcgcctgcgt gtaccggtgg
    16081 tgacccgtgg tgcaggcacc gggctttctg gtggcgcgct gccgctggaa aaaggtgtgt
    16141 tgttggtgat ggcgcgcttt aaagagagcc tcgacattaa ccccgttggt cgccgcgcgc
    16201 gcgtgcagcc gggcgtatgt aacctggcga tctcctaggc cgttgcgccg cataatctct
    16261 attacgcgcc ggatccttcc tcacaaatcg cctgttcaat tggcggcaat gtggcggaaa
    16321 atgccggtgg cgtccactgc ctgaaatatg gtctgaccgt acataacctg ctgaaaattg
    16381 aagtgcaaac gctggacggc gaggcgctga cgctgggatc ggacgcgctg gattcacctg
    16441 gttttgacct gctggcgctg ttcaccggat cggaaggtat gctcggcgtg accaccgaag
    16501 tgacggtaaa actgctgccg aagccgcccg tggcgcgggt tctgttagcc agctttgact
    16561 cggtagaaaa agccggactt gcggttggtg acatcatcgc caatggcatt atccccggcg
    16621 ggctggagat gatggataac ctgtcgatcc gcgcggcgga agattttatt catgccggtt
    16681 atcccgtcga cgccgaagcg attttgttat gcgaactgga cggcgtggag tctgacgtac
    16741 aggaagactg cgagcgggtt aactacatct tgttgaaagc gggcgcgact gacgtccgtt
    16801 tggcacagga cgaagcagag cgcgtacgtt tctgggccgg tcgcaaaaat gcgttcccgg
    16861 cggtaggacg tatctccccg gattactact gcatggatgg caccatcccg cgtcgcgccc
    16921 tgcctggcgt actggaaggc attgcccgtt tatcgcagca atatgattta cgtgttgcca
    16981 acgtctttca tgccggagat ggcaacatgc acccgttaat ccttttcgat gccaacgaac
    17041 ctggtgaatt tgcccgcgcg gaagagctgg gcgggaagat cctcgaactc tgcgttgaag
    17101 ttggcggcag catcagtggg gaacatggca tcgggcgaga aaaaatcaat caaatgtgcg
    17161 cccagttcaa cagcgatgaa atcacgacct tccatgcggt caaggcggcg tttgaccccg
    17221 atggtttgct gaaccctggg aaaaacattc ccacgctaca ccgctgtgct gaatttggtg
    17281 ccatgcatgt gcatcacggt catttacctt ttcctgaact ggagcgcttt tgatgctacg
    17341 cgagtgtgat tacagtcagg cgctgctgga gcaggtgaat caggcaatta gcgataaaac
    17401 gccgctggtg attcagggca gcaatagcaa agccttttta ggtcgcccag tcaccgggca
    17461 aacgctggat gtgcgttgtc atcgcggcat tgttaattac gacccgaccg agctggtgat
    17521 aaccgctcgt gccggaacgc cgctggtggc gattgaagcg gcgctggaga gcgccgggca
    17581 aatgcttccc tgcgagccgc cgcattatgg tgaagaagcc acctggggcg ggatggtcgc
    17641 ctgcgggctg gcggggccgc gtcgcccgtg gagcggttcg gtccgcgatt ttgtcctcgg
    17701 cacgcgcatc attaccggca ctggaaaaca tctgcgtttt ggtggcgaag tgatgaaaaa
    17761 cgttgccgga tacgatctct cacggttaat ggccggaagc tatggttgtc ttggcgtgct
    17821 cactgaaatc tcaatgaaag tgttaccgcg accgcgtgcc tctctgagcc tgcgtcggga
    17881 aatcagcctg caagaagcca tgagtgaaat cgccgagtgg caactccagc cattacccat
    17941 tagtggctta tgttacttcg acaatgcgtt gtggatccgc cttgagggcg gcgaaggatc
    18001 ggtaaaagca gcgcgtgaac tgctgggtgg cgaagaggtt gcgggtcagt tctggcagca
    18061 attgcgtgaa caacaactgc cgttcttctc gttaccaggt accttatggc gcatttcatt
    18121 acccagcgat gcgccgatga tggatttacc cggcgagcaa ctgatcgact ggggcggggc
    18181 gttacgctgg ctgaaatcga cagccgatga caatcaaatc catcgcatcg cccgcaacgc
    18241 tggcggtcat gcgacccgct ttagtgccgg agatggtggc tttgccccgc tatcggctcc
    18301 tttattccgc tatcaccagc agcttaaaca gcagctcgac ccttgcggcg tgtttaaccc
    18361 cggtcgcatg tacgcggaac tttgaggagc aggctatgca aacccgatta accgaagaga
    18421 tgcggcagaa cgcgcgctgg aagccgacag catcctgtgc gcctgtgttc actgcggatt
    18481 ttgtaccgca acctgcccaa cctatcagct tctgggcgat gaactggacg ggccgcgcgg
    18541 gcgcatctat ctgattaaac aggtgctgga aggcaacgaa gtcacgctta aaacacagga
    18601 gcatctcgat cgctgcctca cttgccgcaa ttgtgaaacc acctgtcctt ctggtgtgcg
    18661 ctatcacaat ttgctggata tcgggcgtga tattgtcgag cagaaagtga aacgcccact
    18721 gccggagcga atgctgcgcg aaggattgcg ccaggtagtg ccgcgtccgg cggtcttccg
    18781 tgcgctgacg caggtagggc tggtgctgcg accgttttta ccggaacagg tcagagcaaa
    18841 actgcctgct gaaacggtga aagctaaacc gcgtccgccg ctgcgccata agcgtcgggt
    18901 tttaatgctg gaaggctgcg cccagcctac gctttcgccc aacaccaacg cggcaactgc
    18961 gcgagtgctg gatcgtctgg ggatcagcgt catgtcagct aacgaagcag gctgttgtgg
    19021 cgcggtggac tatcatctta atgcgcagga gaaagggttg gcacgggcgc gcaataatat
    19081 tgatgcctgg tggcccgcga ttgaagcagg tgccgaggca attttgcaaa ccgccagcgg
    19141 ttgcggcgcg ttcgtcaaag agtatgggca gatgctgaaa aacgatgcgt tatatgccga
    19201 taaagcgcgt caggtcagtg aactggcggt cgatttagtc gaacttctgc gcgaggaacc
    19261 gctggaaaaa ctggcaattc gcggcgataa aaaactggcc ttccactgcc cgtgtaccct
    19321 acaacatgcg caaaagctga acggcgaagt ggaaaaagtg ttacttcgtc ttggatttac
    19381 cttaacggac gttcccgaca gccatctgtg ctgcggttca gcgggaacat atgcgttaac
    19441 gcatcccgat ctggcacgcc agctgcggga taacaaaatg aatgcgctgg aaagcggcaa
    19501 accggaaatg atcgtcaccg ccaacattgg ttgccagacg catctggcga gcgccggtcg
    19561 tacctctgtg cgtcactgga ttgaaattgt agaacaagcc cttgaaaagg aataaaaaaa
    19621 tgaaaactaa agtcattctt agccagcaaa tggcgagtgc aattattgcc gcaggtcagg
    19681 aagaggcgca gaaaaataac tggtctgttt ccattgctgt tgccgatgac ggcggtcatc
    19741 tgctggcgtt aagtcgcatg gacgattgcg cgccgattgc ggcttacatc tcccaggaga
    19801 aagcgcgtac cgccgcgctg gggcgtcgtg aaactaaggg ctatgaagag atggtgaaca
    19861 acggacgtac cgcgttcgtg actgcgccgt tactgacgtc gctggaaggc ggcgtaccgg
    19921 ttgttgttga tgggcaaatt attggtgccg tgggcgtttc tggtttaacc ggagcacagg
    19981 atgcgcaggt cgcgaaagtg gcagcagcgg tgttggcgaa ataagcgaaa acgaggagag
    20041 aaacaatgag tcaaaccata acccagggcc gtttacgcat tgacgccaat tttaaacgtt
    20101 ttgtggatgg agaagttttg ccgggcgtgg aactggacgc tgctgcattc tggcacaatg
    20161 ttgatgagat cgttcacgat ctggcaccag aaaatcgtca gttgctggca gaacgcgatc
    20221 gcattcaggc ggcgcttgat gagtggcatc gcagcaatcc ggggccggta aaagataaag
    20281 cggcctataa atctttcctt cgtgaactgg gctacctggt gccgcaaccg gagcgcgtga
    20341 cggtggaaac cacgggcatt gacagcgaaa tcaccagtca ggcggggcca cagctggtgg
    20401 ttccggcaat gaacgcccgc tacgcgctga acgcggcgaa cgctcgctgg ggctcactgt
    20461 acgatgcgtt atacggcagc gacatcatcc cgcaggaagg ggcgatggtc agtggctacg
    20521 atccgcagcg cggtgagcag gttatcgcct gggttcgacg tttcctcgat gaatcactac
    20581 cgctggaaaa cggcagctat caggatgtgg tggcgtttaa ggtggtcgat aaacaattac
    20641 gcatccagtt gaaaaatggt aaagaaacca cgttacgtac tccagcacag tttgtcggtt
    20701 accgtggcga tgccgctgcg ccgacctgca ttttgctgaa aaataacggc ctgcatattg
    20761 aactgcaaat tgatgccaac gggcggattg gcaaagacga tccggcgcac atcaacgatg
    20821 ttatcgtcga agctgctatc agtaccattc tcgactgcga agattcggtc gcggcggttg
    20881 atgcggaaga taaaatcctg ctgtaccgca atctgctggg cctgatgcag gggactctgc
    20941 aagagaaaat ggagaaaaac ggtcggcaaa tcgtacgtaa actgaatgac gatcgtcagt
    21001 acaccgccgc cgatggctct gaaatttctc tgcacggacg ctcgttgctg tttatccgca
    21061 acgtgggtca tttgatgacc attcctgtga tttgggacag cgaaggcaat gaaatcccgg
    21121 aaggtattct tgatggcgtc atgactggcg cgattgccct ctatgattta aaagtgcaga
    21181 aaaactcgcg cactggcagc gtctatattg tgaaaccgaa aatgcacggt ccgcaggaag
    21241 tggcgttcgc caacaaactg tttacccgca ttgagacaat gctcggtatg gcaccgaata
    21301 ccctgaaaat gggcattatg gatgaagaac gccggacctc gctgaacttg cgtagctgta
    21361 tcgctcaggc gcgcaaccgc gtggcgttca tcaataccgg tttcctcgac cgtaccggcg
    21421 atgaaatgca ttcggtgatg gaagctggcc cgatgctgcg taaaaaccag atgaaatcga
    21481 cgccgtggat caaagcctac gaacgtaata acgtgctttc cggtctgttc tgtgggctgc
    21541 gcggtaaagc gcaaatcggt aaaggcatgt gggcaatgcc ggacctgatg gcagacatgt
    21601 acagccagaa gggcgaccaa ctgcgtgccg gggcaaacac agcctgggtt ccgtcaccaa
    21661 ccgctgctac gctccatgcg ctgcactacc accataccaa cgtacagagc gtgcaagcca
    21721 acattgccca gaccgagttc agtgctgaat ttgaaccgct gctggacgat ctgctgacta
    21781 ttccggtggc tgaaaacgct aactggtcgg cgcaagagat ccaacaagag ctggataaca
    21841 acgtgcaggg gattctgggg tacgtggtgc gttgggtgga gcaggggatt ggttgttcaa
    21901 aagtgccgga tattcacaac gtggcgctga tggaagaccg cgcaacgctg cgtatctcca
    21961 gccagcatat cgccaactgg ttacgtcacg gtattctgac caaagaacag gtgcaggcgt
    22021 cgctggagaa tatggcgaaa gtggttgatc agcaaaatgc tggcgatccg gcttatcgtc
    22081 cgatggcggg gaatttcgct aactcgtgtg cttttaaagc tgccagcgat ttaatcttcc
    22141 tcggcgtgaa acagccaaac ggttataccg aaccgttatt acacgcctgg cgtttacgcg
    22201 aaaaagaaag tcattaatag cgtcaaaaaa gccccggcaa gttttattgt cggggctatg
    22261 atagtaactg cggtagtaca tttatcccgg aaataaccgg agcgattatc agggtttaac
    22321 aatattaaat acccgtagcg cattactagc gggcctaaaa caacaatatg gacatcttaa
    22381 cgatactctg cgcctgcgtg tgcggaacag aaacccttac ccgatatact tgttatgcca
    22441 gaggtagcaa ttgcgttagc ggcgtactgt tgctgtaaag tataacgcct atattttaca
    22501 gttcagtcct cttttgctta ttacaacctt aagtatatca agcatataaa gataataaga
    22561 gactgaacaa tatggttacc tggacccaaa tgtatatgcc gatgggagga ctggggctat
    22621 ccgctctggt cgccctgatc ccgataatat tcttcttcgt tgcactcgcg gtattacgtc
    22681 tgaaaggaca tgtcgctgga gcaataaccc ttatattatc tatcctgatt gcgatattcg
    22741 cctttaaaat gccgattgat atggcatttg ctgctgcggg ctatggcttt atttatggat
    22801 tatggccaat tgcgtggatt attgtcgcgg cggtgttcct gtataaatta accgttgcca
    22861 gcgggcagtt cgatattatc cgcagcttgg ttatctccat caccgacgat cagcgtttgc
    22921 aggtgttact gattggtttc tcctttggtg cgttgctgga aggagctgct ggctttggtg
    22981 cgacggtggc gtttggtgcg ttgggcgtgc cgattctggt cgccggtcag gtaacgggaa
    23041 tcgatccgtt ccacattggc gcaatggcgg gacgtcagtt accgttcctg tcggttcttg
    23101 tgccgttctg gctggtagca atgatggacg gctggaaagg ggtgaaagag acgtggccag
    23161 cggcgctggt tgctggggga agcttcgctg tcactcagtt ctttacctct aactatattg
    23221 gtccggaact gccggatatt acttcggcgc tggtgagtat cgtctcactc gctttatttc
    23281 ttaaagtctg gcggccgaaa aataccgaaa cggcaatcag catggggcaa tccgcaggtg
    23341 cgatggtggt aaataagcca tcttctggcg gtcccgtgcc ttcagaatat agtctggggc
    23401 aaatcattcg tgcgtggtca ccgtttttaa tcttaacggt gctggtcacc atctggacca
    23461 tgaagccgtt taaagcgtta tttgctccgg gcggcgcgtt ttattcactg gtgattaatt
    23521 tccagatccc tcatttgcat caacaagtgt tgaaagcggc acccattgtc gcccaaccaa
    23581 cgccaatgga tgcggtgttt aaattcgacc ctctctcggc tggcggcacc gctattttta
    23641 ttgcggcgat tatctctatc ttcatcctcg gcgtgggcat caagaaaggg attggcgtct
    23701 ttgccgaaat gctaattagt ctgaagtggc cgatattgtc gattggcatg gtgctggcat
    23761 tcgccttcgt caccaactat tccggcatgt ccaccacgct ggcgctggta ctggcaggta
    23821 caggcgtgat gttcccgttt ttctcaccgt ttctcggctg gctgggcgta ttccttaccg
    23881 ggtcggacac ttcttcaaac gccctgtttg gttcactgca atcgaccacg gcgcagcaaa
    23941 tcaacgtctc tgacactctg ctggtggcag caaataccag cggcggcgta actggcaaga
    24001 tgatctcccc gcaatctatc gccgtggcct gcgccgcgac gggcatggtg ggccgagaat
    24061 ctgaactgtt ccgctacacc gtgaagcaca gtctgatttt tgccagcgtt atcggcgtta
    24121 tcaccctgct gcaggcgtat gtgtttaccg ggatgttagt ctcgtaaatc aataaagccg
    24181 gatgaaggta aatatcaccc ggctttccat ttgtctgtcg tttatctcgc cagggaaccg
    24241 tttttacgat tggcttcttt atacgtagtc tccgttttac gatgcgaatt gtttaatatt
    24301 tatacaactt ggcttctgtg ctggttataa agtacccact ttcatgagat caattttagt
    24361 atgttattac attgcttcat ctgttgcttt catgacgaat ggcgtttttt ctcttattta
    24421 tatttttcac tagaacaatg agtagatcaa aattcccaaa actcccaaaa acataagatt
    24481 attcgcaaag ctgattttgc agaataatga gaacttttaa gatttgtcga ggatcaattt
    24541 taagcaattt atctccactt gttacataaa agatgccgtt aggttaataa gttaatcttg
    24601 ctataacagt gttcgttact gcctattaat aaagatattt gggatagggt aacgtggatg
    24661 aggattcgaa atcatccgtc ttgcggaaag tgaaatattg gcagatgtta atattatgta
    24721 tgtattttaa ctccaacaac acaccaatca ttttgttaga aataagaaaa tttatggtta
    24781 aataatttgg tgattatgaa gcattaaatc gtaatgccaa atattgttcg ctttggagag
    24841 tattattgcc tgtttgtgtc tgttgagcag aaaaatcatc tgaatttgaa tac
//
LOCUS       DAECNO010000058        24641 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.058, whole genome shotgun sequence.
ACCESSION   DAECNO010000058 DAECNO010000000
VERSION     DAECNO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.058"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(3..79)
                     /locus_tag="KKO08_002408"
     tRNA            complement(3..79)
                     /locus_tag="KKO08_002408"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43..45),aa:Arg,seq:acg)
     gene            complement(83..175)
                     /locus_tag="KKO08_002409"
     tRNA            complement(83..175)
                     /locus_tag="KKO08_002409"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(139..141),aa:Ser,seq:gct)
     gene            complement(491..676)
                     /gene="csrA"
                     /locus_tag="KKO08_002410"
     CDS             complement(491..676)
                     /gene="csrA"
                     /locus_tag="KKO08_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBD6318624.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(911..3541)
                     /gene="alaS"
                     /locus_tag="KKO08_002411"
     CDS             complement(911..3541)
                     /gene="alaS"
                     /locus_tag="KKO08_002411"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBD6318625.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3669..4169)
                     /gene="recX"
                     /locus_tag="KKO08_002412"
     CDS             complement(3669..4169)
                     /gene="recX"
                     /locus_tag="KKO08_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBD6318626.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4412..5473)
                     /gene="recA"
                     /locus_tag="KKO08_002413"
     CDS             complement(4412..5473)
                     /gene="recA"
                     /locus_tag="KKO08_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBD6318627.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5553..6050)
                     /gene="pncC"
                     /locus_tag="KKO08_002414"
     CDS             complement(5553..6050)
                     /gene="pncC"
                     /locus_tag="KKO08_002414"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBD6318628.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6195..7280)
                     /gene="mltB"
                     /locus_tag="KKO08_002415"
     CDS             complement(6195..7280)
                     /gene="mltB"
                     /locus_tag="KKO08_002415"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBD6318629.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7536..8098
                     /gene="srlA"
                     /locus_tag="KKO08_002416"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7536..8098
                     /gene="srlA"
                     /locus_tag="KKO08_002416"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8095..9054
                     /gene="srlE"
                     /locus_tag="KKO08_002417"
                     /gene_synonym="gutE"
     CDS             8095..9054
                     /gene="srlE"
                     /locus_tag="KKO08_002417"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBD6318630.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            9065..9436
                     /gene="srlB"
                     /locus_tag="KKO08_002418"
                     /gene_synonym="gutB"
     CDS             9065..9436
                     /gene="srlB"
                     /locus_tag="KKO08_002418"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBD6318631.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9440..10219
                     /gene="srlD"
                     /locus_tag="KKO08_002419"
     CDS             9440..10219
                     /gene="srlD"
                     /locus_tag="KKO08_002419"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBD6318632.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10324..10683
                     /gene="gutM"
                     /locus_tag="KKO08_002420"
                     /pseudo
     CDS             10324..10683
                     /gene="gutM"
                     /locus_tag="KKO08_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10750..11523
                     /gene="srlR"
                     /locus_tag="KKO08_002421"
                     /gene_synonym="gutR"
     CDS             10750..11523
                     /gene="srlR"
                     /locus_tag="KKO08_002421"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBD6318633.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11516..12481
                     /gene="gutQ"
                     /locus_tag="KKO08_002422"
     CDS             11516..12481
                     /gene="gutQ"
                     /locus_tag="KKO08_002422"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBD6318634.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12478..13992)
                     /gene="norR"
                     /locus_tag="KKO08_002423"
     CDS             complement(12478..13992)
                     /gene="norR"
                     /locus_tag="KKO08_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBD6318635.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14179..15618
                     /gene="norV"
                     /locus_tag="KKO08_002424"
     CDS             14179..15618
                     /gene="norV"
                     /locus_tag="KKO08_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBD6318636.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15615..16748
                     /gene="norW"
                     /locus_tag="KKO08_002425"
     CDS             15615..16748
                     /gene="norW"
                     /locus_tag="KKO08_002425"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBD6318637.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16876..19128)
                     /gene="hypF"
                     /locus_tag="KKO08_002426"
     CDS             complement(16876..19128)
                     /gene="hypF"
                     /locus_tag="KKO08_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBD6318638.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19281..19808)
                     /gene="hydN"
                     /locus_tag="KKO08_002427"
     CDS             complement(19281..19808)
                     /gene="hydN"
                     /locus_tag="KKO08_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBD6318639.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19957..20967)
                     /gene="ascG"
                     /locus_tag="KKO08_002428"
     CDS             complement(19957..20967)
                     /gene="ascG"
                     /locus_tag="KKO08_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBD6318640.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20981..21100)
                     /locus_tag="KKO08_002429"
     CDS             complement(20981..21100)
                     /locus_tag="KKO08_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HBD6318641.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21110..22819)
                     /gene="hycE"
                     /locus_tag="KKO08_002430"
     CDS             complement(21110..22819)
                     /gene="hycE"
                     /locus_tag="KKO08_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBD6318642.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22837..23760)
                     /gene="hycD"
                     /locus_tag="KKO08_002431"
     CDS             complement(22837..23760)
                     /gene="hycD"
                     /locus_tag="KKO08_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBD6318643.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23763..>24641)
                     /locus_tag="KKO08_002432"
     CDS             complement(23763..>24641)
                     /locus_tag="KKO08_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBD6318644.1"
                     /translation="LIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGG
                     IGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAV
                     GLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAA
                     PWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAA
                     WVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMA
                     LVELAVLVVIIVSRGA"
BASE COUNT         5813 a         6933 c         6319 g         5576 t
ORIGIN      
        1 gatggtgcat ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat
       61 ccagctgagc tacggatgca aatggcggtg aggcggggat tcgaaccccg gatgcagctt
      121 ttgaccgcat actcccttag caggggagcg ccttcagcct ctcggccacc tcaccacacg
      181 cctcttacga gtgcttcgaa gaacttgttt atcgctcatc atcgctgcgt ggcgcacata
      241 ttactttctg agacttataa gtcaaacaat ttttcccaca cttttatcgt ttgcacactt
      301 cacgctcaat tagtctgtaa aagcgtcaaa aagagtgttt tatctacaga aaaacggagg
      361 tctggcagat agcagtaatg caaaaaaatg gagacttaag ttgaatgaac gggagtaaag
      421 cgaaaagact atagagtgaa ggagaaattt tgagggtgcg tctcaccgat aaagatgaga
      481 cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc tggtagatct
      541 cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc tggttgccct
      601 ttacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca ccaactcgac
      661 gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc tctctgtatc
      721 ccggcattat ccatcatata acgccaaaaa gtaagcaatg acaaacacat tacatgtaag
      781 cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa cttcaacctg
      841 actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac ggcattgcct
      901 gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct aacgctgcag
      961 gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca ccacccttgc
     1021 cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga tctgtgacgt
     1081 ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc agcacgataa
     1141 ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt ttcggctcaa
     1201 caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc ttactggaaa
     1261 gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct ttttctaact
     1321 gacgcgtacg ttccagtact gagcgcactt tatccgccag attattgcta tcgcctttca
     1381 gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg atagcgcctt
     1441 ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca gagatgatgc
     1501 ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc tcggtggaga
     1561 aatcgcccat gctcagcacg cgtacacgct catcatactt ctcgccgaac agcgccatcg
     1621 cacctttcgc tttcgccgct tcgagattca tgatgttggt ttcgatcggc aaattgcgac
     1681 gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttccggtttc atcgcttcgt
     1741 tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc tgcgatacat
     1801 gagtccccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag tgattcagac
     1861 gaatacgggc gcgacgagcc tcatcaatat cagcctgcac agcgtcgccc actttcagag
     1921 aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc gtatcttcca
     1981 ccgcgaagga gaagttagcg cctttcagtt cgcctttatc accaacctga ccgccggatt
     2041 ccgcatagaa tggcgtttga tccagcacga ccacagcttc ctggcctgca ttgatggcat
     2101 caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc agatggtcat
     2161 agcctttaaa ttcagatgca ctatcaacac ggatcattgc gttgtaatcg gcaccaaagc
     2221 cgctggcttc gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa ccagcttcgt
     2281 caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg aagccatagg
     2341 tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc accagaaagt tttgccagct
     2401 cttcatccag caacgccaga ccgcgctcca gagtacgggc aaactgctct tcttcagtct
     2461 tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca gagcccataa
     2521 cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccg agcatattgc
     2581 cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt tcattggacg
     2641 gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg attacgcgca
     2701 gcgatttatt gctcagatcg gtcgcgccag tgactttcgc taccgcctgg atcaacgtgc
     2761 ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca atacgctcca
     2821 gacccatacc ggtatctaca gacggcttcg gcagcggttc catcgtgcca tctgcctggc
     2881 ggttgaactg catgaagacg atgttccaga tctcaatgta gcggtcgccg tcttcttccg
     2941 gacttcccgg agggccaccc caaatgtggt cgccgtgatc gtagaagatt tcggtgcacg
     3001 ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat ggcgcacctt
     3061 tgttatcgcc gatgcgaata atacgttcgc gcgggatccc gacttctttt tcccagattt
     3121 cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc ggcagggcaa
     3181 accatttttc gctggtcagc agttcccatg caaactgaat ggcatcgtgt ttgaaatagt
     3241 cgccgaagct gaagttgccc agcatttcga agaaggtatg gtgacgcgcg gtgtaaccga
     3301 cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa gtggtagcgc
     3361 gggaataatt acgcttgtcg agcccaagga acacatcctt gaactggttc atcccggcgt
     3421 tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca actacctgat
     3481 gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg ctcttgctca
     3541 taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg tcttaaccgc
     3601 cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa gtaaaatacc
     3661 gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat cagatagcca
     3721 cgatagagca gaaaacgctg gatcttaact ttttctgaaa agacagttgg caaaggttca
     3781 ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc
     3841 atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg acgaatacgt
     3901 gcaggtccat accctttgcg gctacggctg gcgataaagc gcgcaacaaa tcggctgtca
     3961 tcgagatagc catgttcatg gcaccaggca ataacgcgct cgtaatcttc tgccgtagca
     4021 tcaatctctt ctgggccatt tttgcccata atcggtgccg cgagtttacg tcgcagttct
     4081 tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag gcgagcatat
     4141 gccgggcgac gggatgttga ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag
     4201 ggccgcagat gcgccccagg ttttgctgat aaacagattt aatagtcaat gagtatgcag
     4261 aatgttcccg caggcccaat tcgttagagg atcgaggtga gagtaatttt tcacgccagt
     4321 gctggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgtgca
     4381 cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt tctgctactc
     4441 cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac
     4501 gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag gcagttgcat
     4561 tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga
     4621 tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata ccttcgccgt
     4681 agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc accactttca
     4741 cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt
     4801 cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac
     4861 cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact
     4921 gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca
     4981 tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt
     5041 caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt tccagtgcct
     5101 gctcgccggt atccggctgg gagcacagca ggttatcgat atcgacgccc agtttacgtg
     5161 cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcacgct
     5221 gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc ggtccgtaga
     5281 tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc agtgaaagcg
     5341 aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc
     5401 ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt
     5461 cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg
     5521 ttgaaacaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa ttgttgccac
     5581 aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc gccgctgaag
     5641 cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca gacggtgccg
     5701 acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga cacggcataa
     5761 tcagcacgag ccgctttcag tgcacctatc gccatttcca ccacgacggg ttcactcacc
     5821 gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt ggcttcgtta
     5881 ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact
     5941 ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc acgggctttc
     6001 agcgcctgcc caacctgttc gcttaactgc atcagttcac tgtcagtcat cacaacctct
     6061 gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt
     6121 tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc
     6181 cctcttgtaa atagctactg tactcgcgcc agcgccacgg cttgtcctaa ctgccagacc
     6241 gccattgcgt aatgggtgct gtggttgtaa cgggtgatgg tgtagaagtt cggcagaccg
     6301 taccagtact ggtagccggt gccaacatcc agacgcagca ggctggcttg ttgatggttg
     6361 cccagcggct gctgtggcgt taaacctgcg gcggcaagct gcgaaatgct gtacttagtt
     6421 ttgaaaccat ttggcaagcc tggagcctga ccgtttgcca ttatcgcgac ctgatcgcct
     6481 ttcacccagc cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc aaccggatcc
     6541 cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc
     6601 ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc
     6661 tgctcgtcgc gcgccatcag caggaaggtt tccagctcgc cagagaaata ctccgcgcgg
     6721 cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg
     6781 cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg tacgccatac
     6841 acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc
     6901 acgttgtccg gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt cggacctgat
     6961 ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga
     7021 cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc
     7081 attttgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc catctgcatc
     7141 acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt cgtttcagtc
     7201 tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt
     7261 acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg
     7321 taaagcatca caccccgcac aaggaagcgg tagtcactgc ccgatacgga ctttacataa
     7381 ctcaactcat tcccctcgct atacttttat tcaaactttc aaattaaaat atttatcttt
     7441 cattttacga tcaaaataac acttttaaac ctttcaacct gattagatta ggttgccgtt
     7501 tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt actcatggtg
     7561 cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg atggtgaccg
     7621 gcattcttcc gctgttgatt agcctgcagg ttatcatgaa cgcactgatt aattttatcg
     7681 gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt tcccgttacc
     7741 tactgttacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta agcctgggtc
     7801 gctttatgcc ggaaaagtac aaacccagct actacgcggc ggcctcttat agctgccact
     7861 caatgaatgg cctcttcccc catatcaacc ctgcgaactg tttgtttatc ttggcattgc
     7921 cagcggtctg acaacgctga acctgccact tggcccactg gcggtgagtt atctgctggt
     7981 tggtctggtc accaatttct tccgcggctg ggtgaccgat ctgaccaccg ccatttttga
     8041 gaaaaagatg ggcattcaac ttgaacaaaa agttcacctg gcaggagcaa catcatgacg
     8101 cgtattcgga tcgaaaaagg aacgggtggc tggggcggcc cgcttgagct ggaagccacg
     8161 ccgggcaaaa aaatcgtcta tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg
     8221 gcacaactta ctggctggca ggctattgac ggatttaaag aaggtgaacc cgcggaggcg
     8281 gaaattggtg tcgcggtaat cgactgtggc ggcacattac gctgcggcat ctatccgaaa
     8341 cgacgtattc ccaccattaa tatccactcg acgggcaagt ccggtccgct ggcgcagtac
     8401 attgtggaag atatttatgt ctctggcgta aaagaagaaa acatcactgt agtgggtgat
     8461 gcgacaccac aaccctcttc cgtgggccgt gactatgaca ccagtaagaa aatcaccgaa
     8521 caaagcgatg gtttactggc gaaggtggga atgggcatgg ggtccgccgt tgccgtgttg
     8581 tttcagtctg gtcgtgacac catcgacact gtattaaaaa ccattctgcc gtttatggca
     8641 ttcgtctcgg cgctcattgg catcattatg gcttctggcc ttggtgactg gattgcccac
     8701 ggtcttgctc cgctagcgag ccatccactg ggtctggtca tgctagcgct catctgctcc
     8761 ttcccactgc tttcaccttt cctcggccca ggcgcagtta tcgcacaggt tatcggcgta
     8821 ttgattggcg tgcagattgg tctcggcaat attccgccgc atctggcttt accggcactg
     8881 tttgccatca acgcgcaggc ggcttgcgac ttcatcccgg tcggtttgtc gctggcggaa
     8941 gcccgtcagg acacggttcg cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc
     9001 ggcgcgccga ctgtactgat cgcctggttt gtctccagtt ttatctatca atagaggctg
     9061 aaacatgacc gttatttatc agaccaccat cacccgtatc ggcgcgagtg ccactgacgc
     9121 cctcagcgac cagatgctca tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta
     9181 ttgcttcatt cattgccacg gcgagttgaa aggtgcactc catcccggtt tgcaattttc
     9241 actcgggcag catcgctatc cggtgaccgc tgttggcagc gtggcggaag acaaccttcg
     9301 cgaactgggt catgtcaccc tgcgcttcga tggtttaaac gaagcggaat ttccgggcac
     9361 tgtccatgtg gcaggccctg tacccgacga tatcgcgccg ggatcggttt tgaagtttga
     9421 atctgttaag gagtaaaaaa tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt
     9481 aggcgcgttc ctgtgccacg gtctggctgc cgaggggtat cgcgtcgcgg ttgtcgatat
     9541 tcagagcgac aaagccgcaa atgtggcaca agaaattaac gccgaatatg gtgaaggtat
     9601 ggcgtacggt tttggtgctg acgccactag cgagcaaagc gttctggcgc tctctcgtgg
     9661 ggtagatgaa atctttggtc gcgtggattt gctggtctat agcgccggaa tagcaaaagc
     9721 agcctttatc agcgacttcc agctcggcga ttttgaccgt tcgctacaag tgaatctggt
     9781 gggttatttc ctgtgtgccc gtgaattttc gcgtttgatg atccgcgacg ggattcaggg
     9841 tcgcattatt cagatcaact cgaaatccgg caaagtgggc agcaaacaca actctggcta
     9901 cagcgcagcg aaatttggtg gcgtcgggct gactcaatca ctggcgctgg atctggcgga
     9961 gtacggcatt acggtacatt cactgatgct cggtaacctg ctgaaatcgc ggatgttcca
    10021 gtcactgttg ccacaatacg cgaccaagct gggtatcaaa ccggatcaag tcgagcagta
    10081 ttacatcgac aaagtaccgc tcaaacgcgg ctgcgattat caagatgtgc tgaatatgct
    10141 gctgttctac gccagtccta aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg
    10201 cggtcaggtg atgttctgat caacagcgga gatccgttaa ggatctccgt gagactatag
    10261 aatgcctgat gcgctacgct catcaggcat acaggacttc cgccactaca ttaaggaaaa
    10321 gttatggtat ccgcactcat caccgtcgcc gttatcgcct agtgtgcgca actggcctta
    10381 ggcggctggc aaatttctcg ttttaaccgt gccttcgaca cactatgcca gcaagggcgg
    10441 gttggcgtgg gccgttccag cgggcgcttt aaaccgcggg tcgtggtcgc catcgcgctg
    10501 gacgatcagc agtgcatcgt cgacaccttg tttatgaaag gactgactgt cttcgcccga
    10561 ccgcaaaaaa ttcccgcaat taccggcatg catgcgggtg atttacagcc cgatgtgatc
    10621 tttccccatg atccactatc acagaatgct ctatcattgg cgcttaaact gaaacgtgga
    10681 taatttcgtt gtgaatgtta cttgcttgcg aagttatcat tttgaaacct aaatcaggta
    10741 atcacgccca tgaaacctcg tcagcgtcag gccgccattc tggagtatct gcaaaagcag
    10801 ggtaaatgct cggttgaaga attggcgcaa tactttgaca ccacaggcac aaccattcgc
    10861 aaagatctgg tcattctgga acatgccgga accgtcattc gtacttatgg cggagtggtg
    10921 ttgaataaag aggaatccga tccgcctatc gatcataaaa cgctcatcaa cacccacaag
    10981 aaagagctga ttgcagaagc tgccgttagt tttatccatg atggcgattc gatcattctt
    11041 gatgctggca gtaccgtttt gcagatggtt cccctgctct cgcgctttaa taacatcacg
    11101 gtgatgacca acagcctgca tatcgtcaat gcgctatccg aactggataa cgaacaaact
    11161 atcctgatgc caggcggaac atttcgcaaa aaatcggcct catttcacgg gcaactggca
    11221 gagaatgcct tcgagcattt caccttcgat aaattgttta tgggcaccga cggcatcgat
    11281 ctcaatgcgg gcgtaaccac gttcaacgag gtttataccg tcagtaaggc aatgtgcaat
    11341 gccgcgcgcg aagtgatttt gatggcggac tcatcaaagt ttggccgtaa aagccccaac
    11401 gtagtttgca gtcttgaaag cgtcgataag ctaattaccg acgcaggtat cgatccggcg
    11461 tttcgtcagg cgctggaaga gaaagggatc gatgtgatca taaccggaga gagcaatgag
    11521 tgaagcacta ctgaacacgg gacgtcagac gttaatgctg gagttgcagg aagcaagccg
    11581 tttaccggaa cgtctgggcg atgattttgt tcgcgccgcc aatatcatcc tgcactgcga
    11641 aggcaaagtg gtggtttcgg gaattggcaa atcgggccac attggtaaga aaatcgccgc
    11701 aacacttgcc agtaccggca caccggcttt ttttgtccat ccggcagaag cactgcacgg
    11761 cgatctgggg atgattgaaa gccgcgatgt gatgctgttt atctcttact ccggtggcgc
    11821 gaaggaactg gatctgatta ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat
    11881 gaccggcaaa ccgacgtcac cgctgggcct ggcggcgaaa gcggtgctgg atatctccgt
    11941 agaacgcgaa gcctgcccga tgcaccttgc gccgacctcc agcaccgtca ataccctgat
    12001 gatgggtgac gcgctggcga tggcggtcat gcaggcgcgc ggttttaatg aagaagattt
    12061 tgcccgctcc cacccagctg gggcactggg cgctcgcttg ctgaataaag tgcatcatct
    12121 gatgcgtcgt gatgacgcta tcccacaggt agcgttaacc gccagcgtga tggatgcgat
    12181 gctggaactc agccgcaccg gtctggggct ggtggcggta tgtgacgctc aacaacaggt
    12241 acaaggcgtc tttaccgacg gcgatttacg tcgctggctg gtaggcggcg gcgcactcac
    12301 cacgccagtt aatgaagcga tgacgacagg cggcaccaca ttacaggcac aaagtcgcgc
    12361 catcgacgcc aaagaagttc tgatgaaacg caaaatcact gccgcaccag tggtagatga
    12421 aaacggcaaa ctcaccggcg caattaacct gcaggatttc tatcaggccg ggattattta
    12481 atccttcaat cccagacgtt tcgccagccg atgcaggttg gcgacgtcag tttccagcat
    12541 tcgcgcacag gctgcccagt tgtgatgatt ttgtgccagt gcctggcgaa tagtttcacg
    12601 ctggaacgct tctgtcgctt cacgcaggtt ttgcttaaca acgagcaccg ccgccgcttc
    12661 tggcggcggc aacgtcacct caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc
    12721 gccgttgcgg gtggctctcg ccagcactac cgcccgatga atagcatgtt ccagttcgcg
    12781 cacgtttccc ggaaaactgt agtgttgcag taaatttcgc gccccggcac ttaataccac
    12841 gcgggagagc ccctgccgca aacgacactg ctcgcagaaa taccccgcca gcagaatgac
    12901 atcatcgccc cgctcacgca gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa
    12961 caaatcggcg cggaatcgcc ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc
    13021 cagcacgcgc acatcgaccc gcaaactacg gtcatcgcca acgcgctgaa tatcgccata
    13081 ctgcaacacc ctcagcagct tggcctgcaa tgccaacgac aactcgccga tctcatcgag
    13141 aaacagagta ccgttatccg ccatttcaaa cttcccgctg cggttactga tagcgccagt
    13201 aaacgctcct ttcacatgcc cgaacaactc actttccgcc acactttccg gcagtgcggc
    13261 acagttgaga tagaccagcg gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc
    13321 caccagctcc ttaccggttc ccgtctcacc gctgatcaga acgttgagat cggacgccgc
    13381 cacaatctca atctcttttt tcagttgcgt catgccaggg gacaagccaa tcatctgcgt
    13441 ctgtttcacc gcttcaaacg gcgtggcatc gcctggcagc atattctggc tttccagttg
    13501 ttcaatcagc aacgcattgc ttaacgctcc cgccgccagc gcggcaatca gccgtaactc
    13561 ttcgtcgctg aaaacatcga actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat
    13621 caggttttgt ccgacaaaca atggcagacc aacgcaggcg tgaaccttca gactctcctg
    13681 cccaggaatc aaaccgtcat agggatcggg caattcgctg tctgcgggaa agcgcaccac
    13741 atccccggcg cgggcaatcg cttccagccg tggatgccct tccagcgcaa agcgtctacc
    13801 gagtacatcc tttgccagac cgtcgatggc aagcggaata aactgccgcg aatcgtaacg
    13861 tagcaacgca gacgcatcgc actccagcac ctgacgtagc gtggtgatca ggcgctgaaa
    13921 acgatcctga tgaccaatcc cacgctgcaa ttcgatggcg atattcgcca gcacatcaac
    13981 ggaaaaactc atctttgcct cactgtcaat ttgactatag atattgtcat atcgaccatt
    14041 tgattgatag tcattttgac tactcattaa tgagcataat tttatttata gagtaaaaac
    14101 aatcagataa gaaactggca cgcaatctgc aattagcaag acatcttttt agaacacgct
    14161 gaataaattg aggttgctat gtctattgtg gtgaaaaata acattcattg ggtgggtcaa
    14221 cgtgactggg aagtgcgtga ttttcacggc acggaatata aaacgctgcg cggcagcagc
    14281 tacaacagct acctcatccg cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa
    14341 ttcagccgcg aatttgtgca gaacctgcgt aatgaaatcg atctggcgga tatcgattac
    14401 atcgtgatta accatgcaga agaggaccac gccggggcgc tgaccgaact gatggcgcaa
    14461 attcccgata cgccgatcta ctgtactgcc aacgctatcg actcgataaa tggtcatcac
    14521 catcatccgg agtggaattt taatgtggtg aaaactggcg acacgctgga tatcggcaac
    14581 ggcaaacagc tcatttttgt cgaaacacca atgctgcact ggccggacag catgatgact
    14641 tacctgacag gcgacgcggt gctgttcagt aacgatgctt tcggtcagca ctactgcgac
    14701 gagcatctgt tcaacgatga agtggatcag acggagcttt tcgagcagtg ccagcgttac
    14761 tacgccaata tcctgacgcc gttcagccgc ctggtaacgc cgaaaattac cgagatcctg
    14821 ggctttaact taccagtcga tatgatagcg acctcccacg gcgtggtctg gcgcgataac
    14881 ccgacgcaaa ttgtcgagct gtacctgaag tgggccgctg attatcagga agacagaatc
    14941 accattttct acgacaccat gtcgaataac acccgcatga tggctgacgc tatcgcccag
    15001 ggaattgcgg aaaccgaccc acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat
    15061 aaaaacgaaa tcctgaccaa tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg
    15121 atgaataacg tgatgatgcc gaaaatcgcc gggctggtgg aggagatgac cggattacgc
    15181 ttccgtaaca aacgcgccag tgctttcgga tctcacggct ggagcggcgg tgcgatggat
    15241 cgcctttcca cgcgcctgca ggatgcgggt ttcgaaatgt cgctcagcct gaaagccaaa
    15301 tggcgaccag accaggacgc tctggagtta tgccgtgaac acggtcgcga aatcgcccgt
    15361 cagtgggcgc tcgcgccgct gccgcagagc acggtgaata cggtagttaa agaagaaacc
    15421 tctgccacga cgacggctga cctcggccca cgaatgcagt gcagcgtctg ccagtggatt
    15481 tacgatccgg caaaaggcga gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa
    15541 gtcccggata acttcctctg cccggaatgc tccctcggca aagacgtctt tgacgaactg
    15601 gcatcggagg caaaatgagc aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc
    15661 aactggtgaa aaatattcgc aaacaggacg ccactattcc attaaccctg attgccgccg
    15721 acagcataga tgagtacaac aaacccgacc tcagccatgt tatcagtcag gggcaacgtg
    15781 ccgacgacct tacccgccag acggcgggtg aatttgccgg gcagtttaat ctgcgcctgt
    15841 ttccgcacac ctgggtgacg gatatcgatg ccgaagccca tgtggtgaaa agccagaata
    15901 atcagtggca atacgacaag ttagtgctgg caaccggtgc cagcgccttt gtcccgcctg
    15961 tgcctgggcg tgagttaatg ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa
    16021 cgcaactgcg ggatgcccga cgcgtgttga ttgtcggcgg tggtttgatt ggtagcgaac
    16081 tggcgatgga tttttgtcgt gcaggcaaag cggtcacgct aatcgacaac gctgccagta
    16141 ttctggcgtc attaatgcca ccagaagtaa gcagccgctt gcagcatcgg ttgacggaga
    16201 tgggcgttca tctgctgttg aaatctcagt tgcaggggct ggaaaaaacg gattctggca
    16261 ttctggcaac gctggaccac cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg
    16321 gactgcgccc ggaaacagcc ctggcacgac gcgccgggct gacgattaat cgcggcgttt
    16381 gcgtcgatag ttatctgcaa accagtaatg ccgatattta cgcgctgggc gattgcgcgg
    16441 aaattaacgg tcaggtattg ccgttcctgc agccgattca acttagcggg atggtgctgg
    16501 caaaaaatct tctcggcaat aacacgccgc tgaaactacc ggcgatgctg gtgaaaatca
    16561 aaacgccgga attaccgctg catctggcag gcgaaaccca gcgtcaggat ttacgctggc
    16621 agattaatac cgaacgccag ggaatggtgg cgcgcggcgt tgacgatgct gaccagcttc
    16681 gcgcctttgt ggtcagtgag gatcggatga aagaggcatt tgtattgttg aaaacgttgc
    16741 cggtgtaggt gggctactgt gcctaaaatg tcggatgcga cgctggcgcg tcttatccga
    16801 cctacgggga cgcatgtgta ggccggataa ggcatttacg ccgcatccgg caagggtgtc
    16861 caaatgcaac acgctttatc cgttctggac ttcacccgct aaccaacgcg ccgcagcaat
    16921 aaccccctgc cccagagaca aaccgccatc acccgccggt aaactctgtg gaaagagcaa
    16981 tgtgaaatca gcgagataat gcgccagacg tgcacgcagc aaacggttat gaataacccc
    17041 gccgctaaat accagcgtag tgataccacg catcgtggcc tgctcacgca tcaacgcggc
    17101 aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt tgattaacca gtgcctgcca
    17161 gttcagccac tgctgccaga aagtggcgag atccagttga ttgtccaccc gcggcatcgt
    17221 caccggatgc gtcactccgt cgcatgaggc tgcgagcgcc tccagagcac aagccgcttc
    17281 accttcataa cttaacgtgg ctggcgcaca gcccagtgcc gccgccactg catcgaacaa
    17341 ccgcccacac gatgatgcca gcggcgcgtt aattccacgc tcaatggccc gcgccagtac
    17401 gctccagttt tgctgttgca cacttgccgt ttcagggtaa ttctgccact ccggcacaaa
    17461 gcgcaggcac tgcgccagca ggtttcgcca cggctgcttc gccgccaaat cgccacccgg
    17521 aagcgccact gcaggcaagc cgcccaggtg ctcacattcg cgatagttca cccgcaggca
    17581 ctcgccgccc cacaaagcgc cattctcccc cataccgata ccgtcgagcg tcaaagcaat
    17641 gacatcaccg ccatccagcg gccactgatg ctctgccaga cacgccgctg catgggcatg
    17701 atgatgcagt accgtttgcg tcggcagatt catttcgctc gcccactggc tggagacata
    17761 gcccggatgc acatcatgca caatgtattg cggagtgaaa tcgtagatgt tttgcatcag
    17821 gcgtaacgct tcgcgccact gcatctggat gccatcgtca cttaaatcgc ccagatgctg
    17881 actcaacacc gcttgttcac cgcgcaccag gcagaaggta tttttcagat ccgcgccaag
    17941 acacagcaca ggcggaacat ttttaaagcc cggaggcaaa accagcgcat ccggcacata
    18001 cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc actgaatcat ccatccgctg
    18061 cacgatgtcg cggttatgta gcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc
    18121 ctgttcgttg ctgatagctg gtggtttacc gctcaggttg ccggaggtca tcaccagcgg
    18181 gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc gcaggcaaca tcaccccgac
    18241 ttcgttaagg ccaggggcga tatcatcaca aagctcaggt acgtattttt tatccaccag
    18301 cacaatcggc gcagcgggcg tggtaagcaa ctggcgcgca gcgtctggta aactttctgc
    18361 cactggcaac atgactgcca gcggtttcgc cgggcgatgt ttgcgctccc gcagtgtcgc
    18421 caccgcgtta ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc ctttgatggc
    18481 gacaatgttg cccattttta actgtgcgat agctgcctgt aatgccgcct cttgttctgc
    18541 atgttcacca tgacttaccc attcaagatg cgggccacac tccgggcagg ccaccggctg
    18601 ggcgtggaag cgacgatcga gcgggtcgcg gtactctttg tcacaggccg gacatagcgg
    18661 aaacgccgcc atcacggtaa acgggcggtc gtaaggcatc gcgcgaataa tggtgaaacg
    18721 cgggccgcag tgggtacagt tgataaacgg ataacgataa cgccgctcgc ctggggtatt
    18781 catttcggca aggcaagcag ggcaagtggc ggcatccggg acaatttgcg tattcatggt
    18841 gccgccggtg ctctggcgga tggtgaactc ggtgggcagt tgtgaccaga taaacggctc
    18901 acgctcgacg ctatcaatac gcgccagtgg cggacagtgc tgatgcaatt gaacaagaaa
    18961 cgtttccggg tcttccagca gccggacttc tacgccatcg ccgtcattac atacatcgcc
    19021 gtgaagattt aattgctgtg ccagctgcca gacaaacgga cgaaaaccga cgccctgcac
    19081 tttgccacga atacgcagtt ggacaccgca agatgtgttt tttgccattg agttattccc
    19141 gccatcatga attgcgtaac ccgccctgcc ggacacgaca gcgtcgtatc cggcagtctc
    19201 aggtcggcga tcccgcccgc tccgtattct acgaatattt ccgggaattc ctttgatgcc
    19261 agaacagttc tgtaagattt ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc
    19321 agttgttcaa gtttattacg atcgacacaa atcagcgcat gggtcgggca agccgccata
    19381 cacgccgggc cgtcttcacg atggttgcac aggtcgcatt tattggcttc ggctttgtca
    19441 gcccgtacat tcagacccgc gccgctgttg cgaatcacag gacgtaccac cacttccatc
    19501 gcgccatacg ggcaagccac cacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca
    19561 ggaacaaacc ctttatcacg gctgatagca ccattcgggc agacgttagc gcacggtgca
    19621 tcttcacact gacggcaaac tgtcgccgtg gaaatgttca cacctttaat gacatggata
    19681 cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct gattttcctg atgagaaacc
    19741 acgcacgcca cttcacaggt acggcaacca atacatttac tcgcgtcagc aatgatgaaa
    19801 cggttcatca aattctccag caatgacagt taatgcgccg atacattcac aaatcatgcc
    19861 agtttttaat ttgctgttat ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc
    19921 atcgacacta gtgacgatga catgtgatga caatgtttat cgcgaaaggg caatgagtga
    19981 gtcgcggcgg atcagttttc cgctaaaggt tttcggcggt gagaaatccc cgccatcgag
    20041 cataaaaatc agccgtccaa taatttcctg aatcatctca gtcaccggaa tttttacgct
    20101 ggagagcgcc ggaacgatat agggggcaat tgcgatatca tcgaatccga taactgacac
    20161 ctgctctggc accgctacgc cgcgctcgtg caacgctttg atcgcaccta tcgccatatc
    20221 gtcgttactg gcaactaacg cgctaaattt agcccctcgt tcgagcaacg tttctacccc
    20281 ttcggccccg ctggcgggcg tccatttacc gttagcgata agtttttcat tgagcgcaat
    20341 accatgctgc gacagcgcgt ctttataccc ggcaagacgt tcaatgctgg tgggggaatc
    20401 catcgagccg gtaaggaaag caatctcctg atgcccggcg tttatcaact ctgccacggc
    20461 gttaaagctg gtctgtttat gatcgcacca gacgctatgg ctgctattat tgcgcaggcg
    20521 gcgattaagc accattatcg gctgactgtg cgcgtcaatg atgtcatcaa tctcatccac
    20581 gcttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca gatactgaat
    20641 cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc aatagcaact gtcgcccttt
    20701 ctcttccgcc attcgcgcgg catgaaacag taattcacta aaataaatgc cgtgataaag
    20761 cgtgttggtc actaccagcc ccagcgtctg agtactcttc gccgacagat tgcgggccag
    20821 caaatttggg cggtaactgc tctcttctac cgcctgaaac acgcgatctt tagtctcctg
    20881 gctgacgtag ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg
    20941 cttcgccact tccagcatcg tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt
    21001 gtactgcacc gtttgccgat tgtccttgcg caatcggctc cagcggataa gacgaggtcg
    21061 ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct tatttcagcg
    21121 gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc actttgctct
    21181 tcttcttacg cacatcgact acggtcatgc ggtcggtaca ggagtagcaa gggtccaggc
    21241 taccgataat cagcggcgca tcggaaacgg tattgccacg cagcatgtag cgcagggtcg
    21301 gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg ttgtcgccgg
    21361 tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc gcgaaacggt
    21421 gcggaatgta ggtaaagcct tccaccatca gcggaccgcc cggcaggtta tccagaccgt
    21481 agtcgatcat attcagcgcg gtatagacct cgttgattcg cactttcaga cgggaaataa
    21541 cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataaccg acaaacgggt
    21601 gatcggcgcg agtatcgcgg gcgagaccgc tggcgcgaac catcgggcca acgttactga
    21661 aatcgcgggc gatttccggg tccagacgac caatgccgac ggtgcgttgt tccatgttcg
    21721 gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatttgt tgcgccagct
    21781 ggcgggtctg gatcatgtca tctttcagca gatcgcgacg aatcccgccg atcaggttca
    21841 ggccgtaggt tttacgcgca ccggtaagga tctctgccat tttcatggag gtttcacgca
    21901 cgcggaagaa ctgcataaag ccggagtcga agccggtaaa gtgacaggcg aggccgaggt
    21961 tgagcagatg cgggtgcagg cgttccacct ccagcagaat ggcgcggatc atctgcgcgc
    22021 gttctggcac ctgaataccc atcgcgtttt ccaccgacgt ggtgtaggcg gtgctgtggg
    22081 caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgtta taacccatgc
    22141 gggtttccgc cagtttttcc ataccgcgat ggacgtagaa cagacggtag tcggcgtcga
    22201 taatgttttc gccatcgacg aacagacgga agtgacccgg ttcgtcagaa gtgacgtgca
    22261 gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg
    22321 tttcagcatc ggtagtcggt gccggacgct gacgataatc catgctgtct ttacgcagcg
    22381 gataaagttc atccggccag tcatccggca gcaccagacg acgttcatcc ggcagaccaa
    22441 ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc ccacaccgcc gccggaacgc
    22501 gcggcgtcac ggacggatac tccggtttgt tggcgtcaac ttcgacgcga accgttaccc
    22561 aacacttggt gcccttctcc atcgacagca cgtaataaac ggcgtaatga ccattcagtt
    22621 tgcgttcgtc gttaccaaac agcaccgaca gccagccacc ctgcttgtag tagagaaact
    22681 ccaccacttc cggcagatag ttcaccttta cggtgacagt cagctgatct ttggtctgcc
    22741 aggcgtggtc cagcacgacg cctagaaatg cctcattcag cgcggcgaga taatgttgac
    22801 ctaatttttc ttcagacatg ctcaaactct ctttaatcac gccgccagca aggagacgaa
    22861 cgctaaaaat gcaaagccaa acccagccca ggtaatgcgc ggagtaatat caagacgcag
    22921 acgggccatg ctgttttcga acagcgcgat aaccaggacg ccgaccacca gtttgacgat
    22981 ggcaatcacc agcgccagca gcagtccacc gacggtgaag gtttccattt gtccccacgg
    23041 aataaacacc ccgacgaaca tctgcaacac caccagctgt ttcaggctga taccccattt
    23101 catgacgcca aagccgctac cgctgtattc ggagagcgga ccttcctgca actcctgctc
    23161 ggcttccgcc aggtcgaacg gcagtttgcc catttcgata aaagtggcga acgcacaggc
    23221 gcagagcgcc agcaccagcg ggatgctttg gctcagcggc cagtgataaa cggtgtcggt
    23281 aatgttgctg atgttggtgg aaccggcaac ctgtgcggca acccacagac caagcagcaa
    23341 catcggttcg accagcacgc caagcatcgc ttcacggctc gcaccgatag cggtaaacgg
    23401 gctaccggta tccagaccgg aaatggcaaa gaagaaacgc gcgatggcga agagatacag
    23461 taaggtgatc aaatcgccca gttgcggcag cggagaaccg acggtcacca ccggcagcgc
    23521 agtagcgata gtcagcatga cgcccaccat cacatacggc gtcaggcgga acatccagcc
    23581 agaggcatcc gggccaacgc tctggcggcc cagcagtttg ataatgtcgc gatactcctg
    23641 caacacgccc ggcccgcgac ggttatgcag acgggcgcgc gccacgcggg taataccgga
    23701 gagcagcggc gcaacggcaa ataacaccag cgcctgaatt aacggatata aaacactcat
    23761 tctcaggctc ctcgtgaaac aataatcacc accagtaccg ccagttcaac cagcgccatc
    23821 cggcggaaca gcaacgcact accttcgcac tgccagcccg gcactagcga caccggattc
    23881 agccatttgc gcagtttcag cactggcgca aacgcctgtt taaccggcat ggcaaaaccg
    23941 tgagcggtaa tcaccattga tttttcatgg tcgtagccgc acacccaggc cgcaccgcgg
    24001 gaacgtgacg gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg cagcagcggg
    24061 caggcaatca gcagcaatgt gatcattggt tgagaaacgg tggtgttagc aggctccaga
    24121 ggcagaggta cagcagcaga gagcatcggc agcagccacg gcgcagcaac accgccaatt
    24181 acgcagcaaa tcgccagtgc cactacgctt acgctcatca ggagcggcgc acaggtggcg
    24241 ttttcggctt ctttggtacg cggcgcgccg aggaacgtga cgccatagac tttcgccata
    24301 cacatcaccg ccagcgcacc ggtaattgcc agacccacag cgagcagcgg ccccagcaga
    24361 cgggcaacaa acgcgccgct attgctcagt ttgaagaagg attgatagat aacccattcc
    24421 ccggcaaacc cgttcagtgg cggcagcgca gccattgcca tcaacccgac taacatggcg
    24481 atggagataa ccggcatttt cttgccaata ccaccgagtt tttcgatatc gcgatgaccg
    24541 gtacggaacc agacgctccc cgccccgagg aacagtacgc ttttgaacag gctatggtta
    24601 agcagatggt acagaccacc gaccaggcca agagcaatca g
//
LOCUS       DAECNO010000059        24600 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.059, whole genome shotgun sequence.
ACCESSION   DAECNO010000059 DAECNO010000000
VERSION     DAECNO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.059"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(1..374)
                     /locus_tag="KKO08_002433"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="KKO08_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="KKO08_002434"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="KKO08_002434"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBD6318645.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="KKO08_002435"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="KKO08_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBD6318646.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="KKO08_002436"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="KKO08_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBD6318647.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="KKO08_002437"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="KKO08_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBD6318648.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4192..4316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4198..4242
                     /gene="pheM"
                     /locus_tag="KKO08_002438"
     CDS             4198..4242
                     /gene="pheM"
                     /locus_tag="KKO08_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBD6318649.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="KKO08_002439"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="KKO08_002439"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBD6318650.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="KKO08_002440"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="KKO08_002440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBD6318651.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="KKO08_002441"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="KKO08_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBD6318652.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="KKO08_002442"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="KKO08_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBD6318653.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="KKO08_002443"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="KKO08_002443"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBD6318654.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="KKO08_002444"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="KKO08_002444"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBD6318655.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="KKO08_002445"
     CDS             10736..11161
                     /locus_tag="KKO08_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBD6318656.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="KKO08_002446"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="KKO08_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBD6318657.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="KKO08_002447"
     CDS             12185..13621
                     /locus_tag="KKO08_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBD6318658.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KKO08_002448"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="KKO08_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBD6318659.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KKO08_002449"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="KKO08_002449"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBD6318660.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KKO08_002450"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="KKO08_002450"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBD6318661.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="KKO08_002451"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="KKO08_002451"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBD6318662.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KKO08_002452"
     CDS             complement(18751..20397)
                     /gene="fadK"
                     /locus_tag="KKO08_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBD6318663.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20454..20747)
                     /locus_tag="KKO08_002453"
     CDS             complement(20454..20747)
                     /locus_tag="KKO08_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD6318664.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="KKO08_002454"
     CDS             complement(20744..22033)
                     /locus_tag="KKO08_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD6318665.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="KKO08_002455"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="KKO08_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="KKO08_002456"
     CDS             complement(23047..23811)
                     /locus_tag="KKO08_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBD6318666.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24600
                     /locus_tag="KKO08_002457"
     CDS             24127..>24600
                     /locus_tag="KKO08_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBD6318667.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT         6094 a         5940 c         6464 g         6102 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
//
LOCUS       DAECNO010000060        24183 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.060, whole genome shotgun sequence.
ACCESSION   DAECNO010000060 DAECNO010000000
VERSION     DAECNO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.060"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(25..597)
                     /locus_tag="KKO08_002458"
     CDS             complement(25..597)
                     /locus_tag="KKO08_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBD6318668.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="KKO08_002459"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="KKO08_002459"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBD6318669.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KKO08_002460"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="KKO08_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBD6318670.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KKO08_002461"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="KKO08_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBD6318671.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="KKO08_002462"
     CDS             complement(3559..5340)
                     /locus_tag="KKO08_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD6318672.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="KKO08_002463"
     CDS             complement(5333..7105)
                     /locus_tag="KKO08_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBD6318673.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KKO08_002464"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="KKO08_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBD6318674.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KKO08_002465"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="KKO08_002465"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBD6318675.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8664..10356
                     /locus_tag="KKO08_002466"
                     /pseudo
     CDS             8664..10356
                     /locus_tag="KKO08_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10421..11116
                     /gene="queC"
                     /locus_tag="KKO08_002467"
     CDS             10421..11116
                     /gene="queC"
                     /locus_tag="KKO08_002467"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBD6318676.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KKO08_002468"
     CDS             complement(11168..11566)
                     /gene="fadM"
                     /locus_tag="KKO08_002468"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBD6318677.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11660..12031)
                     /locus_tag="KKO08_002469"
     CDS             complement(11660..12031)
                     /locus_tag="KKO08_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBD6318678.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KKO08_002470"
     CDS             complement(12182..14053)
                     /gene="ppiD"
                     /locus_tag="KKO08_002470"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBD6318679.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KKO08_002471"
     CDS             complement(14245..14517)
                     /gene="hupB"
                     /locus_tag="KKO08_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBD6318680.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KKO08_002472"
     CDS             complement(14726..17080)
                     /gene="lon"
                     /locus_tag="KKO08_002472"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBD6318681.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KKO08_002473"
     CDS             complement(17268..18542)
                     /gene="clpX"
                     /locus_tag="KKO08_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBD6318682.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KKO08_002474"
     CDS             complement(18668..19291)
                     /gene="clpP"
                     /locus_tag="KKO08_002474"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBD6318683.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KKO08_002475"
     CDS             complement(19538..20836)
                     /gene="tig"
                     /locus_tag="KKO08_002475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBD6318684.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KKO08_002476"
     CDS             complement(21180..21497)
                     /gene="bolA"
                     /locus_tag="KKO08_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBD6318685.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21802..22380
                     /locus_tag="KKO08_002477"
     CDS             21802..22380
                     /locus_tag="KKO08_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6318686.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22424..23899
                     /gene="ampG"
                     /locus_tag="KKO08_002478"
     CDS             22424..23899
                     /gene="ampG"
                     /locus_tag="KKO08_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBD6318687.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVMLGGLLDYLALRKTHLT"
BASE COUNT         5665 a         6665 c         5828 g         6025 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacgg taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatcgcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcattca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taataatatt gttccgggtt tatatgcgat tgctcaaccg tcttaaccag tatcaacgtc
     8701 tgtggcaacc ttcagccgca aaccgtcacc gtcagcgaac tggccgaacg ctgtttttgc
     8761 agcgaacgcc atgttcgtac gctgttgcgt caggcacagg aggcgggatg gctggagtgg
     8821 caggcgcagt caggacgcgg aaagcgcgga caattacgct ttctggtcac gccggaatcg
     8881 ctacgcaatg cgatgatgga acaggcactg gaaaccggaa agcagcaaga tgtgctggag
     8941 ctggcgcaac tggccccagg tgagctgcgc actctgttac agccatttat gggcggacaa
     9001 tggcaaaacg atacacccac gttgcgtatt ccctactatc gcccgctcga accgctacaa
     9061 ccaggctttt tgcccggccg tgccgagcag catctcgccg ggcagatatt ttccggcctg
     9121 acccgcttcg ataataatac tcagcgcccg attggcgatt tagcgcatca ctgggaaacc
     9181 tctactgaca ggttacgctg ggacttttat cttcgttcaa ccctacactg gcataacggc
     9241 gatgcagtaa aagcctcaca cttacaccag cgattattga tgctgttaca actgccagca
     9301 ctggatcaat tatttattag cgtgaagcgt attgaagtca cccatccgca gtgtctgacc
     9361 ttctttttac atcgccccga ttactggctt gcgcaccggc tggcgagcta ttgcagccat
     9421 ctggcgcatc cgcaattccc actgatcggc actggtcctt ttcgcttaac acaattcaca
     9481 gcggagctgg tgcgcctgga gagccatgat tattaccatt tgcgtcatcc gctgcttaaa
     9541 gcggttgagt actggataac tccgccgctt ttcgaaaaag atttgggaac cagttgtcgg
     9601 catcccgtgc aaatcaccat cggcaaaccg gaggagctgc aacgggtcag ccaagtcagt
     9661 agtggcatca gtttaggttt ttgctattta acgttgcgca aaagtccccg actctccctc
     9721 tggcaggcgc gaaaagtgat ctccattatt catcaatccg gtttattaca aacgttagaa
     9781 gtcggagaaa acctgatcac cgccagtcat gcattactgc taggctggac tattccgcac
     9841 tggcaagtac cggatgaagt caaactaccg aaaaccttga cgctggttta tcacctaccg
     9901 atagaacttc ataccatggc agaacgccta caggcgacac tggcagcgga aggctgtgaa
     9961 ctcacaatta tttttcataa cgcaaaaaac tgggacgaca cgaccctact ggcacacgca
    10021 gacctcatga tgggcgacag attaattggc gaagcaccgg aatatactct ggagcaatgg
    10081 ctacgttgcg atccactatg gccacatgtt ttcgacgctc cagcatatgc acatctgcaa
    10141 tcgacgctgg acgcggtgca agtaatgcct gatgaggaaa accgatttaa tgccctgaaa
    10201 gcggttttta gccagttaat ggcagacgcg acgctgacgc cgctgttcaa ctatcactat
    10261 cgcattagtg cccctcccgg cgtgaacggt gtgcgactga caccgcgcgg ctggtttgaa
    10321 tttaccgaag cctggcttcc cgcgccgtcg caatgaatag ctggtccggg ttaatcgcag
    10381 gcgttaccat aacgctttta ttgttcaagc aggattatct atgaaacgtg ctgtcgttgt
    10441 gttcagtgga ggccaggatt ccaccacctg tctggtgcag gcattacaac aatatgatga
    10501 agtccattgc gtgacgttcg attacggtca gcgacatcgc gcagaaatcg acgtggcacg
    10561 cgaactggcg ctgaaactgg gagcacgcgc gcataaggtt cttgatgtaa cgctgctcaa
    10621 cgagctggcg gtcagcagcc tgacacgtga cagcattccg gtgcctgatt atgaacctga
    10681 agccgatggc atcccgaata cgtttgtccc agggcgtaat attttgttcc tgacgctggc
    10741 ggcaatatat gcgtatcagg taaaagcaga agccgtaatt accggcgtct gtgaaacgga
    10801 tttctccggc tacccggatt gccgcgatga gtttgtgaaa gcactaaacc atgccgtcag
    10861 tttgggcatg gcgaaagata ttcgttttga aacgccgctg atgtggattg ataaagcgga
    10921 aacctgggcg ctggcagatt attacggcaa actggattta gtccgtaacg aaacgttgac
    10981 ctgctataac ggcattaaag gcgacggttg cggtcattgt gcggcatgta atttacgcgc
    11041 caacggtttg aatcattatc tggccgataa accgacggtg atggcagcga tgaagcagaa
    11101 aaccgggttg aggtaattat tccgggtgtc gccggatgcg gcttgagcat ccggcaccac
    11161 aaaacgttta cttaaccatc tgctccagct tttcgcgcaa ttccccttcc agagctaatg
    11221 ctttctgcgt tttaagatca atacaaacaa acgtaataag cgcatccgct accacctgcc
    11281 cttccggctc cagtgtaatg acctggttta agatgccgct tttaccgttt aattgctgca
    11341 actgactggt aatagttaac aggtcgctta ataccgctgg gcgacgatag ttaatattga
    11401 tattgaccac gacgaaggcg atgttatggg ccgtcatcca ctgaaaactg tcgctatttt
    11461 ccaccccatc ccagcgggct tcttcgagaa attcaaggta gcgggcgttg ttgacgtgct
    11521 ggtaaacgtc gagatgatat ccacgaactt tgatttgtgt ttgcatagcg caataacctt
    11581 acgttgttgt tataagtaca gaggtcataa ctggtatgac ctcttcagtc tggcaaaaaa
    11641 ttgccactat gcaaattaat tacagggtta ataccgccag attacgttcc accagcgaat
    11701 tgcccatccc cggcacctgc tttagatcct ccaccgtttt aaacggaccg tactcttcgc
    11761 gataactgac aatcgcctgc gctttcttca ggccaacgcc attcatcgcg cgggctagct
    11821 cttccgcgct ggcattatta atgctgaccc gggtgccttc ttcgtcactg gctttcgccg
    11881 gtactgctgc tttactttgt gctgcaggag cttccgcttt ggtttctacc gccgtcggtt
    11941 tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc agggacaggg
    12001 taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg ttaacagtgg
    12061 aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa tggaaaaggc
    12121 cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg cggcttcagg
    12181 attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc aggttactca
    12241 tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc atcgcttttt
    12301 tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga accacattac
    12361 cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt ggcagtgcaa
    12421 acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc tcgccaaatt
    12481 tcagaccggc agcctgcata gcttccgcac ctttgccggc tttcaaatca accagcagtt
    12541 tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt gccttaactt
    12601 gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg acgcgcagca
    12661 cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccgac gcgccgtttt
    12721 cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc aactcttccg
    12781 gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca gcttgctctg
    12841 caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc tgcagcgcat
    12901 agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca cgtacttcgt
    12961 ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg aaaccgaccg
    13021 aagatttgat gacaccagac agttggcctt tttctttcag accagcattt ttcagttcgt
    13081 cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca gagataatat
    13141 cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc tcatcaagta
    13201 ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta cgctgcggct
    13261 gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg ctaaccggtt
    13321 gctgcatcgt tgcggcaacc agcttgatgt aactcacgcg gaattgttcc ggcgtcatga
    13381 aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca ggctgcttcg
    13441 ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc gcgaccagtg
    13501 ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaacg ccgttaatca
    13561 gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg gtcatcccca
    13621 tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc tggaaggctg
    13681 gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc agctcacgag
    13741 cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga cgcagggttt
    13801 tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt tgctgcatac
    13861 gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc tggtcattca
    13921 cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg gtcaatatga
    13981 acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca gccgtgcgta
    14041 agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct cacgcaacgt
    14101 atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag cccacaagac
    14161 acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc ttgaacttcg
    14221 tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt tacctgcacg
    14281 gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg ggttgcggcc
    14341 agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg ctacatcatc
    14401 cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac ggccagccgc
    14461 agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt tattcactct
    14521 tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa cgcgcagcag
    14581 ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc cgatccgcta
    14641 tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca gccctgtttt
    14701 tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg ccagacggtt
    14761 cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga atgtccagat
    14821 cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac ggaattagca
    14881 ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca ccgatcggca
    14941 gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga accgggttac
    15001 cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga ccatctttcg
    15061 gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa tcagggttga
    15121 tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata gactcctgca
    15181 tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca catgcggttt
    15241 caatggtcag caagtcaccg cccacttccg tccacgccag accgattacc tgaccgacac
    15301 ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag tcatgcaggt
    15361 tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc ttaaccgctt
    15421 tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca cgggtgtagt
    15481 aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt ttcagtgcat
    15541 tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt tcatcttcgg
    15601 tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga atgttcatgg
    15661 agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc aggtagtggt
    15721 cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag gccggatcgc
    15781 ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcgga tttttcacgc
    15841 ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa gtacggcggt
    15901 gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca tatttacgcc
    15961 cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc ggccctacca
    16021 ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg tactcaagga
    16081 tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc tgcgcctgac
    16141 gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc cagtcgatat
    16201 aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc ttctgcaact
    16261 ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt ttgcgcttca
    16321 gggcttcgtt ttcgtccggc acgtcgtcca tttcaccgag ttctttctga atagctttca
    16381 tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta acgcggttgc
    16441 gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc atcagatatt
    16501 ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc agtttcagcg
    16561 gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta ttcagcgacg
    16621 tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac tggctgattg
    16681 cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc agatactccg
    16741 ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg cgctgtaacc
    16801 cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat atagaggcca
    16861 cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct tctttctgcg
    16921 cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg atagattttt
    16981 cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg cgcagcggca
    17041 atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct cttagtttaa
    17101 tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa catgtacgtc
    17161 agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa ttaaagggga
    17221 gataaaatcc cccctttttg gttaactaat tgtatgagaa tggttaatta ttcaccagat
    17281 gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc atcaattacc
    17341 gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta catggtatcg
    17401 agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg cgccatcgct
    17461 ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc ttccagatta
    17521 aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat ctgaatcaga
    17581 gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat gaactcaggg
    17641 ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc gccttcgctt
    17701 gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc ggtttctaca
    17761 cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa caggatctta
    17821 gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg cggtggaaca
    17881 gcagctaccg taccttcgat cagtttcaac agtgcctgct gtactccttc accggaaacg
    17941 tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
    18001 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag cttctgaatg
    18061 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcggccatg
    18121 gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt accggaaccg
    18181 gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct ggtatcgccg
    18241 ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
    18301 tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
    18361 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
    18421 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
    18481 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
    18541 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
    18601 aggcgcaagc cgcttacagc gttgtgccgc cctagataag tatagcggca cagttgcgcc
    18661 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
    18721 gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
    18781 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
    18841 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc atcacgcgcg
    18901 aattcggcag gcaaaaacgt ttaccttttg cccctgcggt caacaagaaa gcgcccatcg
    18961 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
    19021 agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga tagatatctt
    19081 tttctgggtt ttccgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
    19141 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
    19201 aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
    19261 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaaa
    19321 cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt gttatgggta
    19381 cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac cgaaagaggc
    19441 ttttcgcacg gctccatgca aaattcatga caaaaaaaag cccaccacct ggcggtgacg
    19501 ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg ttcatcagct
    19561 cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct tcaacagcct
    19621 gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg ctgtagaact
    19681 cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg cctttcacgc
    19741 gctcttcgtc agctttcagc tcgttggtgc ggataacttc gcccagcagc aggccaacaa
    19801 ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga gcttgttttt
    19861 cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg ctgtcgatca
    19921 gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc tgagacttaa
    19981 cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact tcagcgcgca
    20041 gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca gtcagttccg
    20101 gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct ttacctttca
    20161 ggttttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct tcgccagctt
    20221 tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc atcgccagta
    20281 cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg gtgaagtcga
    20341 tggttacgcg gtcttctgct tcaacagcgc cgtctttttc tttccaggtc gcctgctgtt
    20401 tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg atcggttttt
    20461 caacttcgat tgcttccaga ccctgcagtt caacttccgg ataaacttca aactctacag
    20521 agtaagtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt gcgccagccg
    20581 gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg tcacccagaa
    20641 cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc actttgcctt
    20701 tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt ttaacagcgg
    20761 tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct tgagtggttt
    20821 caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa ctcttacccc
    20881 gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat cacattccct
    20941 gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg gcatcacttt
    21001 tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc ccgggcaatt
    21061 ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg cccgcaggcc
    21121 gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca aatgcgtttt
    21181 tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc ctgcaaccct
    21241 tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt agagagttcc
    21301 tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc cgtaaaacga
    21361 tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt gtgacgatag
    21421 ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt ttcttctatc
    21481 cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat ccatcccttt
    21541 aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt aggtttcgtc
    21601 ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca caggcctcgc
    21661 atcagcgttt cttttaccag gggtgattat ttcgtcaaca tgatgaattt acatgcctta
    21721 cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca aacacgctta
    21781 agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt tgctctgttt
    21841 atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac gattacactg
    21901 ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc cgatcagcgt
    21961 accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct gaccgcctcc
    22021 cgcgctctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc gcgtggttac
    22081 atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact gtatgctgac
    22141 gtgtcccagg gcaacgtgcg ctacaacatc gctaccaaag cggatatcgc catcatcgct
    22201 accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa cgttgaaggt
    22261 gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct gaccgatacc
    22321 atcgctgata tgtctcagga caccagcatt cacgaattca tcaagcagaa cgcgcgttaa
    22381 tttctgccct ctggcccggt gcaagccggg cctgtagacg cccatgtcca gtcaatattt
    22441 acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg cttccgggct
    22501 accgctcgcc ctgacatccg gcaccttaca ggcctggatg acggtcgaaa atatcgatct
    22561 caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat tcctctggtc
    22621 accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt ggctgctcgc
    22681 cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac caggcaccca
    22741 actccgctgg atggcggcgc tggcggtggt gatcgctttt tgctctgcct cccaggatat
    22801 tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg cgggcgcggc
    22861 aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcctag ccctgtggct
    22921 ggcagataaa tggttgggct ggcagggcat gtactggttg atggcggcgc tgttgatccc
    22981 ctgtattatc gcgacgttgc ttgcaccaga accaaccgac accattcctg tgcccaaaac
    23041 gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata atgcctggct
    23101 tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga gcctgacaac
    23161 cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg taaacaaaac
    23221 gcttggctta ctggcgacca ttgttggcgc attgtacggt gggattttga tgcagcgcct
    23281 gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta acgctggtta
    23341 ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg tctttttcga
    23401 aaacctctgt ggcgggatgg gcacatcagc ctttgtcgcg ctgttaatga cgctgtgtaa
    23461 taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg tagggcgagt
    23521 ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga cattctatct
    23581 attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc gccagacgct
    23641 tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccgg caggttatgc
    23701 ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt tacttctgtt
    23761 gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc tggaagtcgg
    23821 ggttttagtc gccctttctg gcgtcatgct tggtggtttg ctggattatc tggcgctacg
    23881 aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac tcgccgtaat
    23941 tattacggct aaataaacat cagcatcgct tattaatatt tgttcttttg tgcggcttag
    24001 cgtttggaaa ttattgacgc catttataaa ttctattttt cttacacgat tcagctaatg
    24061 agtctttatt ttctcaccac ccagttgtca ctttaatgat aattatttgt taaataattg
    24121 ttttatttcg cattggttat accaattgcc cgccaagact ccttctgcac tccccttttg
    24181 tta
//
LOCUS       DAECNO010000061        23858 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.061, whole genome shotgun sequence.
ACCESSION   DAECNO010000061 DAECNO010000000
VERSION     DAECNO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.061"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(2..298)
                     /locus_tag="KKO08_002479"
     CDS             complement(2..298)
                     /locus_tag="KKO08_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBD6318688.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="KKO08_002480"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="KKO08_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBD6318689.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="KKO08_002481"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="KKO08_002481"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBD6318690.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="KKO08_002482"
     CDS             complement(1784..2323)
                     /locus_tag="KKO08_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBD6318691.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="KKO08_002483"
     CDS             complement(2353..3096)
                     /locus_tag="KKO08_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBD6318692.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="KKO08_002484"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="KKO08_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="KKO08_002485"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="KKO08_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBD6318693.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="KKO08_002486"
     CDS             complement(4151..4657)
                     /locus_tag="KKO08_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBD6318694.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="KKO08_002487"
     CDS             complement(4703..5203)
                     /locus_tag="KKO08_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBD6318695.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="KKO08_002488"
     CDS             complement(5289..5468)
                     /locus_tag="KKO08_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBD6318696.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5849..6655)
                     /gene="trpA"
                     /locus_tag="KKO08_002489"
     CDS             complement(5849..6655)
                     /gene="trpA"
                     /locus_tag="KKO08_002489"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBD6318697.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6655..7848)
                     /gene="trpB"
                     /locus_tag="KKO08_002490"
     CDS             complement(6655..7848)
                     /gene="trpB"
                     /locus_tag="KKO08_002490"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBD6318698.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7860..9221)
                     /gene="trpCF"
                     /locus_tag="KKO08_002491"
     CDS             complement(7860..9221)
                     /gene="trpCF"
                     /locus_tag="KKO08_002491"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBD6318699.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPCIKDARLLASVFQTL
                     RAY"
     gene            complement(9222..10817)
                     /gene="trpD"
                     /locus_tag="KKO08_002492"
     CDS             complement(9222..10817)
                     /gene="trpD"
                     /locus_tag="KKO08_002492"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBD6318700.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10817..12379)
                     /gene="trpE"
                     /locus_tag="KKO08_002493"
     CDS             complement(10817..12379)
                     /gene="trpE"
                     /locus_tag="KKO08_002493"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBD6318701.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12401..12494)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12471..12515)
                     /gene="trpL"
                     /locus_tag="KKO08_002494"
     CDS             complement(12471..12515)
                     /gene="trpL"
                     /locus_tag="KKO08_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBD6318702.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12653..13534
                     /gene="yciV"
                     /locus_tag="KKO08_002495"
     CDS             12653..13534
                     /gene="yciV"
                     /locus_tag="KKO08_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBD6318703.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13531..14151
                     /locus_tag="KKO08_002496"
     CDS             13531..14151
                     /locus_tag="KKO08_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBD6318704.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14252..15124
                     /gene="rluB"
                     /locus_tag="KKO08_002497"
     CDS             14252..15124
                     /gene="rluB"
                     /locus_tag="KKO08_002497"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBD6318705.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15164..15754)
                     /gene="cobO"
                     /locus_tag="KKO08_002498"
     CDS             complement(15164..15754)
                     /gene="cobO"
                     /locus_tag="KKO08_002498"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBD6318706.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15751..16509)
                     /locus_tag="KKO08_002499"
     CDS             complement(15751..16509)
                     /locus_tag="KKO08_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBD6318707.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16729..17778
                     /gene="sohB"
                     /locus_tag="KKO08_002500"
     CDS             16729..17778
                     /gene="sohB"
                     /locus_tag="KKO08_002500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBD6318708.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17814..18065)
                     /locus_tag="KKO08_002501"
     CDS             complement(17814..18065)
                     /locus_tag="KKO08_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBD6318709.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18445..21042
                     /gene="topA"
                     /locus_tag="KKO08_002502"
     CDS             18445..21042
                     /gene="topA"
                     /locus_tag="KKO08_002502"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBD6318710.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21252..22226
                     /gene="cysB"
                     /locus_tag="KKO08_002503"
     CDS             21252..22226
                     /gene="cysB"
                     /locus_tag="KKO08_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBD6318711.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22521..22685
                     /locus_tag="KKO08_002504"
     CDS             22521..22685
                     /locus_tag="KKO08_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBD6318712.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22688..22855
                     /locus_tag="KKO08_002505"
     CDS             22688..22855
                     /locus_tag="KKO08_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318713.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22969..23064
                     /locus_tag="KKO08_002506"
     CDS             22969..23064
                     /locus_tag="KKO08_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318714.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23228..>23858
                     /locus_tag="KKO08_002507"
     CDS             23228..>23858
                     /locus_tag="KKO08_002507"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBD6318715.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVG"
BASE COUNT         6011 a         5879 c         6086 g         5882 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccga agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccatac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgtc accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaacc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc gctaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaaat atattttgag gaacattctg gatatattaa caattacctg aggaatatgt
     5641 gacttagaga aaattattga ttttactggt gttatgttgc gggattaatt tgtttgatta
     5701 tattactgtt gggcggaaaa atgacgtaag ttgacgttcg accggggtaa gcgaaacggt
     5761 aaaaagataa atattaaatg aatttaggat ttttccggct tcattaaaga aagttaaaat
     5821 gccgccagcg gaactggcgg ctgtgggatt aactgcgcgt cgccgctttc atcggttgta
     5881 caaaaacttt cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt
     5941 taacaatggc cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct gcttttacct
     6001 gatccggggc ggaaatacca aacccctgca atggaggtgc agcgttgtac tctttcagct
     6061 tcgcaaccag atgattgagg ggtaacgcgg cgcggttttc tgcgccggtc acgcccgctc
     6121 gtgacagcaa atatgtgtaa ccacgaccgt aagaggctat ctggcgcagc aggtcgtcat
     6181 cggcatttgg cgggcagatg aagataggtg cgacattatg gcgcaacgcg gcctggcgga
     6241 agggcgcgga ctcttcaact ggcacatcgg caaccagcac cgaatcgacg ccgactttct
     6301 cgcactgggc ataaaactca tcaatgcctt tgttaaacac cagattggca tacatcaaca
     6361 ggccaatggg aatggtcggg tgtttctggc gaatcagtgc cagcatttca aaacattgtg
     6421 ccggagtcac acctgccgca aaggcgcgca gagtggcgtt ttgaatggtc gggccatccg
     6481 ccagtgggtc ggagaagggg atacctaact ccagcgcgtc agcaccggct tcaattagcg
     6541 tatcgataat tttcaatgac tgctcaatgc ccggatcacc gagcgtgacg aaaggaacga
     6601 atgcgccttc tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat
     6661 ttcccctcgt gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga
     6721 aaggttaacc accagtagct gctctttatc cgggttttcg cgcatcattt tcagcgcatg
     6781 ggccagggcg tgggaggatt ccagcgccgg gatgatccct tcgtgcaggc acagcgtttt
     6841 gaaggcttca agggcttcat catcggtaat agacacgtaa tcagcgcgtc cagtgctgtt
     6901 aagatacgcg tgttgtggac cgacagacgg gaaatccagt ccggcggaga tggagtaaga
     6961 ttcttcaatc tgcccgtctt cggtttgcat catcggcgct ttcataccga aataaatgcc
     7021 cacgcgacca tgttttagcg gtgcgccgtg ctcgccagtt tcgataccgt gaccaccagg
     7081 ctccacacca atcaggccga cgttggtttc attgatgaaa tcagcaaaca tgccgatggc
     7141 attcgaaccg ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag
     7201 aatctgcgct ttggtttctt cgccaatcat ccgctgaaac tcacggacaa tggtcggata
     7261 aggatgcggg ccagccgcgg tgcccagcat atagtgcgcg gtttcataac tgccggacca
     7321 gtcgcgtagc gcctcattac aggcatcttt cagcgtggcg gaaccgctat gtaccgggat
     7381 cacttccgca cccattaagc gcatacgaaa aacgttaggc gactggcgtt caacgtcttt
     7441 ggcacccata taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac
     7501 gccatgctga ccggccccgg tttcggcgat gatttcggtt ttacccatcc gcttcgccag
     7561 caacgcctgc cccagcacct ggttggtttt atgtgcgccg ccgtgcagca aatcttcacg
     7621 cttgagatac agcgtggtgt tcgtcccggc tgtaatgttc tggcatttgg tcagtgcggt
     7681 tggacgcccg gcatagtttt tcagcaggtc gttgaactgg gcttgaaatt caggatcttt
     7741 ttgcgcgctg acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac
     7801 gtacatgccg ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct
     7861 taatatgcgc gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt gatgcacggt
     7921 tgcgactcta cagcagaatt aaaatcaagt ccggcgcagc cggtttgtgc cgcttccacg
     7981 cagttatctg cgcctaagcc ccccgccagc agaacgttgc caagcgattg accatttaat
     8041 agtgaccagt caaaacgttg cccgcttcca ccctggccgt tgtctaaaac atatttatca
     8101 acgtgctgaa actcgcgggc gggcagggtt tcaccgacgc ttaatgcttt ccagatggca
     8161 acatgtgctg gcagagcttc acgcagcgta tcgatataca gctgatcttc attaccatgc
     8221 agttgcactg ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga
     8281 ttgcggaaca cgccaacata ctgcaacggt gccgcagcca tcacttcctg cgcctgttca
     8341 acgttgacgc aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg
     8401 tcataagctg ctttagcatc ttgcccacgc gtcaggccac atactttatt ctcacccagc
     8461 aacacccggc gcacggcggc gtgcaaatcg tcatgggcca tcaacgccga accaatcaga
     8521 aaaccgttag cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg
     8581 ctgattaccg ttacgttgtg ccccagtttc ggcgcaagct cgcgggtacg gttgagatca
     8641 atcgacaaat cacgcagatc gcggttgttg atgccaacga cctttgcccc taatgcaatg
     8701 gcgcgctcca gttcctcttc attactgact tcggtcagca cccccatctc cagactgtga
     8761 gcgacggctg caagctggcg atattggtcg tcatccagta ctgaaagcat taataagcag
     8821 gcatcggcct ggtaatagcg cgccagatag atctggtaag ggtcgataat gaagtcttta
     8881 cataaaatcg gctgcggggc gatttggctg acgatgagga ggaaatcaaa gctcccctga
     8941 aaatatttct catcagtcag caccgaaatt gccgaagcgt aatgtttata aatggcggca
     9001 atgcgtgccg gatcgaaatc atcacggatc acgccttttg acggcgacgc ttttttacac
     9061 tccagaataa acgccgtgcg tgcaccctgt agcgcatcat aaaaatgtcg tgtgctcggc
     9121 tgaacctcat tctgaaaact ggccagcggt tgctgctgtt tgcgggtttc tacccaaatc
     9181 gccttgtctg cgacgatttt cgctaaaacg gtttgcatca tttaccctcg tgccgccagc
     9241 gcggtaactc tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg
     9301 gcttgcagat cttcatggcc atgcaggcgc attaacatgg cgacgttcgc agcgacggct
     9361 gcttcatggg cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct
     9421 tcaggtgttc cgcctgccag ttgctcctgg tggtagggag tcaggccaaa atcttcagcg
     9481 gtcaattgat agctcttaat tttgccgtca tgcagctcgg caacgattgt cggcgcgtgt
     9541 aatgaaactt catccatccc gccgctgtgc accaccgccg cgcgttgata ccccagcacg
     9601 cgcaaggttt cggcaatcgg cagcaccagt tccggactat aaacaccaat taacgccagc
     9661 ggcggatgcg ccgggttaat caatggcccc agcacattga acagggtgcg ggttttcagt
     9721 tgctggcgaa ccggcatcgc gtggcggaat ccggtgtgat atttcggcgc aaagaggaaa
     9781 catacgccta actcgtccag cgcctggcgc gatttattgg cgttcatatc aagattaata
     9841 ccgaatgccg ccagcagatc cgacgagccg gatttactgg agacgctgcg gttaccgtgt
     9901 ttcgccactt tcagcccaca ggccgcggcg acaaacgcac tggcggtaga aatattgata
     9961 ctgttgctgc cgtcaccgcc agtaccgacg atatcagcaa acagataatc cgggcgcggg
    10021 aacggcgctg cgttttccag tagcgcggtt gctgccccgg cgatctcgtt cgggtgctca
    10081 ccgcgaattt tcatgctcac cagcgccgcc gccagttgtt ccggcttcag ctcgccacgc
    10141 accaccgctg aaaacagctg gtggctttct tgttggctaa gcgtctgcgc ctgatacagt
    10201 ttttccagaa tcggttgcag cgtgttggct ggctctagtt tctgctgcgc ccaggccagc
    10261 gtttgttcca gcaggcgagc gccctgggtg gtgagaatgg attccggatg gaactggaat
    10321 ccacaaacgc gatccgcatc gtgacgtact gccatcacca tgccattaaa atgggcgttg
    10381 atggttaaac cggccggaat gttactgcca accagcgagt gataacgcgc caccggcagc
    10441 gggtttgtta atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg
    10501 tgaagaattt cgcccgcctg accgacatag cccccgtaag cttcgacaat tgcctgatgt
    10561 ccgaggcaaa tgccaataat gggcagcttg ccacgcaagc gggtgaggag ttccggcata
    10621 caaccggctt cgctcggcac accggggcca ggagaaagca tcagtaccgg attgctcatc
    10681 gtcgccaggc gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacgtta
    10741 tgaccattgc tgcgcaactg atctgccagg ttgtacgtaa aagagtcgat attatcgagc
    10801 agcagaatgt cagccatcag aaagtctcct gtgcatgatg cgcggtggca atagcgcgca
    10861 gtacagcgcg ggctttgtta cgggtttcgt cggcttccga ctgcggaaca gaatcaagga
    10921 ctacaccagc acccgcttgc acggtggcga taccgttttc caccagcgcc gagcggatca
    10981 caatgcaggt gtcgagatcg ccatgcgcgg tgaaataacc taccgcgccg ccgtagctgc
    11041 cgcggcgacg accttccgcc tcggcaatta actgcatagc gcgtactttc ggcgcaccgc
    11101 ttaacgtccc catattcata caggcgcgat aagcgtgcag ggcgtcaaga tcgtgacgca
    11161 gttcgccgac tacgcgagag acgaggtgca tcacatagga ataacggtca actttggtga
    11221 gatcggcgat gtagcggctg ccaggggtgc aaatgcgtgc cagatcatta cgggcgagat
    11281 caaccagcat cagatgttca gacagctctt tatggtcagt acgcatttcc agttcaatac
    11341 ggctgtcgag atctctgtcc agtgaaccat cggcgcgacg accgcgtggg cgtgttccgg
    11401 caatcgggta gatctcaatc tggcggctgg tggcatcata cttgagcgag ctttccggcg
    11461 acgcgccaaa tagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac
    11521 tctttttcag cacgtaatag gccgccagcg gtgacgggca gggcagagag aaacggcgag
    11581 atggcaccac ctggaaaatt tctccagcgc gaatcgcttt ttgcaacaaa cgcactacgc
    11641 caccgaactc ttcatcgctc tgattacatt cacaacgcat atgcggcacg gaaaccactg
    11701 gcagcggcgg cgcggcttcg gtcagttgct gacgtagttc gttcaggcga gcagtgagac
    11761 gttgtttttc ttcttcattc ggagcaaaca ggctggcctg aatacgggtg ctttttttct
    11821 gatggtcaat caccatcagc gtttcagcga gataaaaaca gaaatcaggg cagttatttt
    11881 ccgctgacag ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag aacaggccgc
    11941 cgaagaacat tgcttctcgt tcttccttcg gtacattcaa cagattctgc aataaacgga
    12001 aagcgtcaaa aaccgaaagg gagcataagc gggcgtcttc atccagcagt ggactgacag
    12061 gggggaagcg cagcacacgg cagtttggtg attgttcact ttccacaccc gcaggcaggg
    12121 cgttatccag tagtgccagc agggcttcgc cattgccgga aagtgcctga attgtgacgg
    12181 tgtcacctaa tgctgtaatg cgcagcgcac tgtctatcag cagcaggctt tttaaatcat
    12241 ctttgctgtc gatatctgcg gattccagca gcagcgttgc cggacgattc ccacacaact
    12301 ggtgaaaaag cgcagtcggg ttgtcgcgat aagcgccttc gcaggttagc agttcgagag
    12361 tcggtttttg tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta
    12421 ggcgggctgg gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg
    12481 cgccaccaac ctttcagtac gaaaattgct ttcattgtcg ataccctttt tacgtgaact
    12541 tgcgcactag ttaactggtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc
    12601 agacccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga
    12661 cacgaattat gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct
    12721 gacgccagaa gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac
    12781 cgaccatgac accacagctg caatcgcgcc agcaagagag gaaatttcac gttcgggact
    12841 ggcgctgaat cttattcccg gcgtggaaat ttccacggtc tgggaaaatc atgaaattca
    12901 tattgtcggg ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca
    12961 gacagaacgg cgcaatcagc gggcgcagca aattgccgaa agacttgaaa aagcgcaaat
    13021 acctggggcg ctggaaggcg cacaacgact ggcgcagggc ggcgcagtga cgcgcggtca
    13081 ttttgcgcgt ttccttgtgg agtgcggcaa agccagttca atggcagatg tctttaaaaa
    13141 gtatctggcg cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc
    13201 tattgatgtc attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa
    13261 tcttagtgct aaatggctga aaagattggt tgcgcatttt gccgaacacc acggcgacgc
    13321 gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg ccacacttgc
    13381 gcgtcagcat catttatggg catcacaagg atctgatttt catcagccat gcccgtggat
    13441 cgaactgggt cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc agctatggga
    13501 acagccgcag aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat
    13561 aacccgcagc aacgtctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt
    13621 gtctatccaa ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa aaacgccatg
    13681 gagcgtattt gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc
    13741 gatctttctg aactctcgac ctattcattc gtggataacg ttgcgtttcg tttgatgaaa
    13801 aacaacacgc cgggcaacta taccttcatc ctgaagggga cgaaggaagt gccacgccgt
    13861 ctgttgcagg agaaacgcaa aaccatcggt atgcgtgtgc cttctaaccc gattgcgcag
    13921 gcgttgcttg aagcactggg cgaaccgatg ctttccactt cgctaatgct gccaggcagc
    13981 gaatttaccg aatccgatcc cgaagaaatt aaagaccgtc tggaaaaaca ggtagatttg
    14041 attattcacg gcggctatct cgggcagaaa ccaacaacgg ttattgatct taccgatgat
    14101 acgccggtcg tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata agtgcgtaac
    14161 tctgtatact acgcggcctt aaaatggcgc tatacggcgc cattgattct cctgttcgac
    14221 gcctgtgaag gcgacaccaa aaggaagctc tatgagcgaa aaattacaga aagtgctggc
    14281 ccgcgctggc cacggctccc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag
    14341 tgttgatggc aaaattgcta aactcggcga tcgcgttgaa gttacccctg ggctgaaaat
    14401 tcgtatcgat ggtcatctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct
    14461 ggcctattac aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccgac
    14521 ggtgtttgac cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgtctgga
    14581 cgttaacacc tgtggtctgc tgctgttcac caccgatggt gaactggcaa accgtttaat
    14641 gcatccaagc cgtgaagttg aacgtgaata cgccgtgcgt gtatttggtc aggttgacga
    14701 cgcgaaactg cgtgatttga gtcgtggcgt gcaactggaa gatggtccgg cagcttttaa
    14761 aaccatcaag ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga
    14821 aggccgtaac cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg tgagccgcct
    14881 gatccgtgtt cgttacggtg atatcccact gccaaaaggt ctgccacgcg gtggctggac
    14941 ggagctggac ctcgcccaga ctaactatct gcgcgaactg gtggagcttc cgccagaaac
    15001 cagctctaaa gtcgctgtag aaaaagaccg tcgtcgcatg aaggctaatc agattcgtcg
    15061 tgcggtgaaa cgtcacagtc aggtgagcgg cagtcgtcgt tctggcgggc gtaataacgg
    15121 ttaatcaaag tatgccggac gtcatatccg gcatttttac agattaataa tcaatcccta
    15181 tttgcgcctt aacaccggca tcaaacgcat gtctgatggg gcgcagctca cttaccgtat
    15241 ccgccagttc aagaatatcc cgatgacagc ctcgacccgt gataatcacc gtctgttgat
    15301 gtggacgttc atttaacgcc tgcaccactt cttccagtgg caaatagtca tacgccacca
    15361 tatacgtcag ttcatcaagc aaaaccatat ccagtgagga atcagcaagc atccgctttg
    15421 catgttgcca gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg
    15481 taaagcccgt tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc
    15541 cattaggcca ggtgccttta ataaactgca cgacgcccac tttttttccg tgaccaactg
    15601 cgcgtgttgc cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga
    15661 tgataatacc gcgttcatcc tgggcctggg ccacacgagc atctactttt tctttcactc
    15721 gctgctgacg ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg
    15781 ttgggcgtca aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta
    15841 gagcggcatg atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct
    15901 tgcgcgcatt gcggtgcgcg taccgcctgg gttaatgcag ttgacgcgca ggcgctgctg
    15961 atattcatcg gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc
    16021 accccagttg gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc
    16081 gtccgatttg agtaataaag gaagcagtgc ctgggtgagc ataaaggtgg cattaacgtt
    16141 gacctgcatg acgtcctgcc agacctgcgg attttgttcg ctcattgggc aaacatcgcc
    16201 gagcaatccg gcattatgca aaacaccatc cagacgcgga taattaacga caatgcgctg
    16261 tgccagttgt tggcaatttt cggacgtgca ggtcagcaaa tcgagaataa accactgtgg
    16321 ctgacgccca gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg
    16381 gcccaacaga atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacgaccaat
    16441 cccatcgctg gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg
    16501 gtaatgcatg gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact
    16561 ttggggcttt atgcctgaaa cagaagggca tttcaatcag cctggggcga taacaccgca
    16621 aaatggatac tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc
    16681 ctgttgtaaa ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg
    16741 tctgaatatg gtttgttttt ggctaaaatc gttaccgttg tgctagcgat tgcggcgatt
    16801 gccgccatta ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc
    16861 aacaatctca gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac
    16921 tcacatcagc aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa
    16981 gcagcaaaag cgaaagccaa actgggcgag gtggcaactg acagtaaacc tcgcgtctgg
    17041 gtgctggatt ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata
    17101 acggcggtac tcgcagcatt caaaccgcag gatcaggttg tgctccgtct ggaaagccct
    17161 ggtggcatgg tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa
    17221 aacattcctt taactgttac ggtagacaaa gtcgctgcca gcggcggtta catgatggcc
    17281 tgtgtggcgg acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg
    17341 gcgcaaatgc ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac
    17401 accgccgggc agtataagcg cacgctgacc ttgctgggtg aaaataccga agaagggcgg
    17461 gagaaattcc gcgaagagct gaacgaaacg catcagttat ttaaagattt tgtgaagcgt
    17521 atgcgtccgt ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag
    17581 gcggtagaga aaggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg
    17641 atggaaggcc gtgaagtggt taatgtacgc tatatgcagc gtaaacgact cattgaccga
    17701 ttcaccggca gcgcggcaga gagcgccgat cgattgttgc tacgctggtg gcagcggggt
    17761 caaaagccat tgatgtaaaa gacaaacgcg aggctaagac ctcgcgtttt gctttaatca
    17821 accagatgat atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg
    17881 gtgcttttgg ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc
    17941 acgccattac gctgcgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca
    18001 cgaatgattt tgttggcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg
    18061 ttcattattt actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa
    18121 tataccacgc tggtggcaag agcgccttac tggcaacttt ggattttgca tgctaataaa
    18181 gttgcgtatc ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt
    18241 gcttgacatt cgaccaaaat tccgtcgtgc tatagcgcct gtaggccaag acctgttaac
    18301 tcagtcacct gaattttcgt gaacagagtc acgacaaggg gttgatatcc gcagagagcg
    18361 agtccatatc ggtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga
    18421 agaatcaaat taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca
    18481 aaagccaaaa cgatcaacaa gtatctgggt tgtgactacg tggtgaaatc cagcgtcggt
    18541 cacatccgcg atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc
    18601 accaagacgg ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg
    18661 gttgacccgt ggcacaattg ggaggcgcac tatgaagtgt tgcctggtaa agagaaggtc
    18721 gtctctgaac tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt
    18781 gaccgcgaag gggaagccat tgcatggcac ctgcgggaag tgattggtgg tgatgatgcg
    18841 cgctatagcc gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaac
    18901 aaaccgggtg agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac
    18961 cgcgtggtgg ggtatatggt ttcgccgctg ctgtggaaaa agatcgctcg tggtctgtct
    19021 gccggtcgtg tgcagtcggt ggcggtccgc ctggtggtag agcgtgagcg tgaaattaaa
    19081 gcgttcgtac cggaagagtt ctgggaagtc gatgccagca cgactacgcc atctggtgaa
    19141 gcgttggcgt tgcaggtgac tcatcagaat gacaaaccgt tccgtccggt caacaaagaa
    19201 caaactcagg ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa
    19261 gacaaaccga caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct
    19321 gccagcaccc gtcttggttt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat
    19381 gaagcaggct atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta
    19441 aatatggttc gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg
    19501 aatcagtacg ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac
    19561 gtcaatgtga tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag
    19621 ttaatctggc gtcagttcgt tgcctgccag atgaccccag cgaaatatga ctccacgacg
    19681 ctgaccgttg gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gtgctttgat
    19741 ggctggacaa aagtgatgcc tgcactgcgt aaaggcgatg aagatcgtat cttaccggca
    19801 gtcgataaag gcgatgctct gacgctcgtt gaactaacac cagcccagca ctttaccaag
    19861 ccgccagccc gtttcagtga agcatcgctg gttaaagaac tggaaaaacg tggtatcggt
    19921 cgtccgtcta cctatgcgtc gatcatttcg accattcagg atcgtggcta tgtgcgagta
    19981 gaaaatcgtc gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg ccttgaagaa
    20041 aatttccgcg agttaatgaa ctacgacttt accgcgcaga tggaaaacag cctcgaccag
    20101 gtggcaaatc acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcacc
    20161 cagcagttag ataaagctga aaaagatccg gaagagggtg gtatgcgccc gaaccagatg
    20221 gttctgacca gcattgactg cccgacttgt ggtcgcaaaa tggggattcg cacagcgagt
    20281 accggggtat tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaacc
    20341 accattaacc tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agatgctgaa
    20401 accaacgcgc tgcgcgcaaa acgtcgttgc ccgaaatgcg gcacggcgat ggacagctat
    20461 ctcatcgatc cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac
    20521 gagatcgaag agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa
    20581 aaatgtggct ctgaaatgca cctgaaaatg gggcgtttcg gtaaatacat ggcctgcacc
    20641 aacgaagagt gtaaaaacac acgtaagatt ttacgtaacg gcgaagtggc accaccgaaa
    20701 gaagatccgg tgccattacc tgagctgccg tgcgaaaaat cagatgctta tttcgtgctg
    20761 cgtgacggtg ctgccggtgt gttcctggct gccaacactt tcccgaaatc gcgtgaaacg
    20821 cgtgcgccac tggtggaaga gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt
    20881 tatctggccg atgcgccaca gcaggatccg gaaggtaata agaccatggt tcgctttagc
    20941 cgtaaaacca aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca
    21001 gcattttatg ttgatggcaa atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt
    21061 ttttataaac aaagggtcgc gaaagcggcc cttttttatt gcatattatt ttttcttcac
    21121 acctatacac taaggctata aatgatatag tggttatagt tagcaccttt tttattatta
    21181 aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg
    21241 gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc
    21301 tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc agtaaacaag
    21361 tcagaatgct ggaagacgag ctaggcattc aaattttttc ccgaagcggc aagcacctga
    21421 cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag
    21481 tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata
    21541 tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa ggctttattg
    21601 agcgttatcc ccgcgtttct ttgcatatgc accagggccc gccgacacaa attgctgatg
    21661 ccgtctctaa aggcaatgcc gatttcgcta tcgccacaga agcgctgcat ctgtatgaag
    21721 atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc
    21781 cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat
    21841 ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc gcagggttaa
    21901 cgccgcgtat cgttttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag
    21961 ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat cccgaccttg
    22021 tgcgcgttga tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta
    22081 gtactttttt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc
    22141 gtgatgtcgt tgatgcggct gtcgcattgc gctccaatga agaaattgag gtcatgttta
    22201 aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc
    22261 gaaggtgccg gaatatattc tcttttgtta cttatttcta taccgtgcac aatattttta
    22321 tccctcatat ttatagggta aattacctgt aaagtagtgt ctaattaccc gcctcgcaaa
    22381 ttccagtttt caaatatcta tttcaggtca aaagattgaa ttattcactg gagacgattc
    22441 gtaaattcgc tggatttttg actaaagttt ctttaggatt tgtctcatcg tatgattaat
    22501 tacaccaatc attcaacgtc ttgctgataa gtgatggcga ttatatgagg ttagcaatgc
    22561 cttctggaaa tcaggaaccc cgccgtgatc ctgaattaaa acgtaaagcc tggctggcgg
    22621 tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg aaagtgtggg
    22681 gataactatg gtcggtcagg agcaactgga gtcgtcacca ttatgccagc atagtgacaa
    22741 tgaaccagaa acgaaacggg aatgttccgt cgttattcca gacgactggc aactaacatc
    22801 gcagcagcaa gcctttatag aactgtttgc tgaagatgat cagccgaaac aataattatc
    22861 atcattctta ttacccattt ttaatgaatt aaagggcttt taatacaccg cagcaataac
    22921 agcttgagtt atctcaacac aaaataataa ccgttaaggg tgtagcctat gatcaacaca
    22981 aatatgaaat attggtcctg gatgggcgcg ttttctctgt cgatgctctt ctgggccgaa
    23041 ctcctctgga tcattactca ctgatccttg accccgctgc ggcggggttg tcatttgctt
    23101 tgccacaagg tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt
    23161 tgtgttatct ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg
    23221 aggagctatg tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa
    23281 aacttaccac tactacagcc tgccgcttgc tgctaaatca ctgggcgata tcacccgtct
    23341 acccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcagt
    23401 taccgaagag gatatccacg cactggcggg atggctgaaa aatgcccatg ctgaccgtga
    23461 aattgcctac cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt
    23521 tgatctggcg gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg caaaggttaa
    23581 cccgctctca ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga
    23641 tgatgaggca tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg aacgttatgt
    23701 gttcctgaaa tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc caggcacagg
    23761 catttgccat caggttaacc tcgaatatct cggcaaagca gtgtggagcg aattgcagga
    23821 cggtgaatgg attgcttatc cggatacact cgttggta
//
LOCUS       DAECNO010000062        22622 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.062, whole genome shotgun sequence.
ACCESSION   DAECNO010000062 DAECNO010000000
VERSION     DAECNO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.062"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..434)
                     /gene="pgrR"
                     /locus_tag="KKO08_002508"
     CDS             complement(<1..434)
                     /gene="pgrR"
                     /locus_tag="KKO08_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBD6318716.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
     gene            572..1492
                     /locus_tag="KKO08_002509"
     CDS             572..1492
                     /locus_tag="KKO08_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBD6318717.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1492..2428
                     /locus_tag="KKO08_002510"
                     /pseudo
     CDS             1492..2428
                     /locus_tag="KKO08_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            2446..3084
                     /locus_tag="KKO08_002511"
     CDS             2446..3084
                     /locus_tag="KKO08_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6318718.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            3375..4103
                     /gene="mpaA"
                     /locus_tag="KKO08_002512"
     CDS             3375..4103
                     /gene="mpaA"
                     /locus_tag="KKO08_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBD6318719.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4078..5037)
                     /gene="ycjG"
                     /locus_tag="KKO08_002513"
     CDS             complement(4078..5037)
                     /gene="ycjG"
                     /locus_tag="KKO08_002513"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBD6318720.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5156..5662
                     /gene="tpx"
                     /locus_tag="KKO08_002514"
     CDS             5156..5662
                     /gene="tpx"
                     /locus_tag="KKO08_002514"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBD6318721.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(5706..7247)
                     /gene="tyrR"
                     /locus_tag="KKO08_002515"
     CDS             complement(5706..7247)
                     /gene="tyrR"
                     /locus_tag="KKO08_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBD6318722.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(7395..8456)
                     /locus_tag="KKO08_002516"
     CDS             complement(7395..8456)
                     /locus_tag="KKO08_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBD6318723.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(8453..9850)
                     /locus_tag="KKO08_002517"
     CDS             complement(8453..9850)
                     /locus_tag="KKO08_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBD6318724.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10006..11004
                     /locus_tag="KKO08_002518"
     CDS             10006..11004
                     /locus_tag="KKO08_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD6318725.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11115..11987)
                     /gene="ompG"
                     /locus_tag="KKO08_002519"
                     /pseudo
     CDS             complement(11115..11987)
                     /gene="ompG"
                     /locus_tag="KKO08_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(12060..13141)
                     /locus_tag="KKO08_002520"
                     /pseudo
     CDS             complement(12060..13141)
                     /locus_tag="KKO08_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13155..13814)
                     /gene="pgmB"
                     /locus_tag="KKO08_002521"
     CDS             complement(13155..13814)
                     /gene="pgmB"
                     /locus_tag="KKO08_002521"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBD6318726.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(13811..16078)
                     /gene="ycjT"
                     /locus_tag="KKO08_002522"
     CDS             complement(13811..16078)
                     /gene="ycjT"
                     /locus_tag="KKO08_002522"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBD6318727.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16075..16698)
                     /locus_tag="KKO08_002523"
     CDS             complement(16075..16698)
                     /locus_tag="KKO08_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD6318728.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(16695..17117)
                     /locus_tag="KKO08_002524"
     CDS             complement(16695..17117)
                     /locus_tag="KKO08_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBD6318729.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSRLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(17139..17914)
                     /locus_tag="KKO08_002525"
                     /pseudo
     CDS             complement(17139..17914)
                     /locus_tag="KKO08_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(17932..18984)
                     /locus_tag="KKO08_002526"
     CDS             complement(17932..18984)
                     /locus_tag="KKO08_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBD6318730.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19015..19857)
                     /locus_tag="KKO08_002527"
     CDS             complement(19015..19857)
                     /locus_tag="KKO08_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBD6318731.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(19844..20725)
                     /locus_tag="KKO08_002528"
     CDS             complement(19844..20725)
                     /locus_tag="KKO08_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBD6318732.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(20746..22029)
                     /locus_tag="KKO08_002529"
                     /pseudo
     CDS             complement(20746..22029)
                     /locus_tag="KKO08_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(22043..>22622)
                     /gene="ycjM"
                     /locus_tag="KKO08_002530"
     CDS             complement(22043..>22622)
                     /gene="ycjM"
                     /locus_tag="KKO08_002530"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBD6318733.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5575 a         6085 c         5511 g         5451 t
ORIGIN      
        1 acgcctgcat caaaacgttc agaaacgaca tcggtcaaac cataatcaat ggtaagctga
       61 atatcaattt caggatgcga tttcaggaat gtgcgcatgg ctggtaacaa tatcgttttt
      121 gctgcatgtt ctacagtagt aatacgtatt gtcccggatg ggcggttctg cagatcgctc
      181 agggatgtca gggcagaatc tatgtcatgc aatatagggc caagaacaga caaaagatgc
      241 tcgcccgctt cagtgggtac aacgctgcgc gtggttcgcg tcagaagccg caatcccaat
      301 cgttcttcta tacggcgcac tatctggctt aaagctgact gcaccatgct caggcgggct
      361 gctgcgcgag tgaagctacg ctcctctgca acgacgacaa acgccatcag atcagcgatt
      421 tcttcacgct tcattcagta tctccataca atctattaat catttaaatg ataggtctaa
      481 tcatgattta gggtctaatc aacttaatga tcaatatcta tagtgatatc accaaccgtc
      541 aaaacgaacc gacaactcca tgaggaactg aatgatgaat aataaagtca gcttcactaa
      601 cagcaataat ccaaccatct ctttgtctgc agtgatctat ttccctccaa aatttgatga
      661 aacccgtcag tatccggcaa ttgtggtatc tcacccaggt gggggcgtta aagagcaaac
      721 ggccggaacc tatgccgaaa agctggcgga aaagggattt gtcactattg cttatgacgc
      781 atcttatcaa ggtgaaagtg gcggcgagcc gcgtcagtta gaaaacccat acattcgtac
      841 tgaagacgtc agtgccgtga ttgattacct gacaacgctt tcttatgtcg acaatacccg
      901 aattggtgcg atgggaattt gtgccggtgc aggatataca gccaatgctg caattcagga
      961 tcgccgcatc aaggccatcg gtaccgttag tgcggtcaat attggttcga tgttccgcaa
     1021 tggttgggaa aacaatgtga aatctattga tgctctgcca cacattgaag ctggctcaaa
     1081 cgcccgaacc agcgatatta gcagcgaaga atacgccatt atgccactgg ctccaatgaa
     1141 ggaggccgat gcgccgaacg aagaactgcg tcaggcctgg gagtattacc acacacctcg
     1201 tgcgcagtat ccaacagcac cgggttacgc tactttgcgc agccttaacc agattattac
     1261 ctatgatgct taccatatgg cggaagtgta cctgactcag ccgatgcaga ttgtggcggg
     1321 cagccaagca gggagcaaat ggatgagtga cgacctgtac gatcgggcat caagccagga
     1381 taaacgctat cacattgttg aaggcgcaaa ccatatggat ctttatgacg gcaaagccta
     1441 tgtggctgaa gcaatttcag tattagctcc gttctttgag gaaactctgt aatgaaacat
     1501 attcaaatcc gcaatagtga tatggcctgg catattgctg ccaatattca gtttccacca
     1561 aattttgatg agtcaaaaca gtatccggcg attatcagcg tccatccttt tggcagttgt
     1621 aaggagcaga cgtctggcaa tatctatggc aaggcattgg ctgaaaaagg ctatgtcgtt
     1681 ctcgcttatg acgccagttt ccagggagaa tctggtgggt agcctcgttg gatagaagat
     1741 ccgacacagc gcgtagagga tattagccgt gttatcgatt atgcagtaac tctgccatat
     1801 gttgatgctg aacgcattgg cgtgctgggt gtgtgggggg ggggggggtg gctatgccat
     1861 taatgccgcg ctgacagaaa aacgaattaa agctgtagtg agtattacgg gtgttaatat
     1921 cgggcgtctt tttcgggaag gtttcagcaa ttatgatccg attggcgtat tgaatgcaat
     1981 ggcgtcacaa cgagcaaaag aggctcgtgg tggagaatta cagatcaacg aattacttcc
     2041 cgccagcctg gatgcagcaa aagcccatgg actgaccgaa cgcgatgttt atgaagcaac
     2101 tgactactat aaaacaccac gcgggcaaca accgggaggt gcgacaaaaa tgttgttctc
     2161 acatgcacag aaaacgctgg cctgggatgc cttcgcattt actgaagtgt tactgaccca
     2221 gccggtgatg gtagtagttg gtgaaaaagt cggtgccttc ggtgcttatc gtgatggact
     2281 ggaagtctat ggccgggcga tggtttcgca ggatcgtcag ttagtttcgt tacccgattt
     2341 ctcacattat gaactatatg acaaaccgga agcagttcag gaggtgctgg caaaagttat
     2401 tccttttttc aacacccatc tgggataagt ttcatgagaa atattatgaa aaatgtactg
     2461 attcttggtg ctggcggtca gatagcccgc catgtgatta atcaactggc agataaacaa
     2521 actattaaac aaactttatt tgcccgtcag ccagcaaaaa tacacaaacc ctatccaact
     2581 aatagccaga ttattatggg ggatgtactg aatcatgcag cactgaagca ggccatgcag
     2641 ggacaggata ttgtgtatgc caaccttacg ggggaagatt tagatattca ggcaaattca
     2701 gtgatcgctg cgatgaaagc ctgtgatgtt aaacgtctga tctttgttct ttccctcggt
     2761 atttatgatg aagttccagg aaaatttgtc gaatggaata atgcagttat tggtgaaccc
     2821 ctaaaaccat tccgcagggc tgcggatgca attgaggctt ctggactgga atatacaatt
     2881 cttcgccctg cctggctgac tgatgaagat attattgatt atgagcttac ctctcgcaat
     2941 gaaccattca aaggtacgat cgtatcacgt aagagtgtcg cagcattaat tacagatatc
     3001 atcgataaac ctgagaaaca cattggtgag aatataggca tcaatcaacc cgggactgat
     3061 ggcgataagc cattcttcat gtaaatcaat tatatagata ccatcgtact gtactatgtg
     3121 cagtacgatt tattaaactt attaaggtga actgatcaat cagatgcgat taacatgtgt
     3181 agaaccagta agaataaaat acgccatgta tataacatta cgacaattct aatatctgca
     3241 catcaatgaa aatgcaaaca acttccggca aaaaagtgat acaagatcga tgacatagcg
     3301 ttgaagaaaa ggatttgata atccgcaaaa tttgcacaac actgcctgta atacttcctg
     3361 aaccagagac gatcatgacc gtaacccgct cacgcgccga acgcggcgca tttccgcccg
     3421 gaacagaaca ttacggacgt tcattattgg gcgcgccgtt aatctggttt cctgcccctg
     3481 cagccagccg cgaaagtggt ttgattctgg ctggcactca cggtgatgaa aactcttcgg
     3541 tcgtcacgct ctcctgtgct ctgcggactt tgacaccttc tttgcgccgc catcatgtgg
     3601 tgctgtgtgt gaatcctgac ggctgccagc tggggttacg ggccaatgct aatggtgtgg
     3661 atttaaacag aaactttccg gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga
     3721 acagcgccgc tgaagaacgt gatgtcgtat tactgacagg tgacaaacct ggctccgaac
     3781 ctgaaaccca ggcgctgtgc cagcttatac accgcattca acctgcctgg gtggtctcat
     3841 tccacgatcc actggcttgt attgaagatc ccagacacag cgaattaggc gaatggctgg
     3901 cccagtcgtt tgaattgcct ctggtaacca gcgtcggtta tgaaacgcct ggctctttcg
     3961 gcagttggtg tgcagatctc aacttgcatt gcatcaccgc agagtttccg cccatctcct
     4021 ccgacgaagc cagcgaaaaa tatctctttg cgatggcaaa cttgctgcgc tggcatccta
     4081 aagatgcaat tcgcccgtcg tgaactgaag cgccggttcc acatctaccg ccagccaggt
     4141 cggtccgtca agatcggcga aactgacctg cggcaccagc ggtaaagcgg cgctaatggc
     4201 acgagaggta cacaacatgc agcccagcat cagactgaaa ccttgtgcac gcgcttcagt
     4261 cgccagcgct tccgtcagac caccggtttt atcgagctta atgttaacca tctcatagcg
     4321 ccctttcagc gccttcagat tgctacgagt atgacaactt tcatcagcac aaatcggcaa
     4381 cggatgaata aaattctcca gcgccgcatc gtcctgcgca ggaagcggtt gttcaagcat
     4441 cgcaacgcct aaatccgcca atagctggca acgcgccgcc aacccttctg cacgccagga
     4501 ttcatttgca tcaacgatca gcgtggcatc gggcacagcg gtgcgaattg ccaccatccg
     4561 ctcactgata agatggttat ccagcttcac tttcagtaat ttcgcgcctg cctgccagag
     4621 tgttgatgca ctattggcca tctgatcagg cgtaccgatg acaaccgtct gtgcagtgat
     4681 aactgtctcg ggaagcgtta tgccgatcaa atcagccagc gattgctgct gttttcgcgc
     4741 cgcgagatcc cacaatgcac aatccagcgc attacgtgct gcgccggcag ggagaatttt
     4801 ttgcaactcc tcccgtgtca gccctttctc taattgcggc acgacgctca taatttgcgc
     4861 cattaccgag gcatcacttt ctccataacg cggatacggc gtgcattcgc cggtgccttt
     4921 aataccctct tcttccagtt caaccaccac cacgcgcgct tcactgcgac ttccccgggc
     4981 aatcacaaac ggggtatgta agggccaggc ttcctcaaat accttaacga ttctcatctt
     5041 tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa tagcgtttgc tgtgttattg
     5101 acagttagca taaactaggt gtgacgttaa ctatatgtaa acaggaaaga taatcatgtc
     5161 acaaaccgtt catttccagg gcaacccggt tacagtcgcc aattccatcc cgcaggcggg
     5221 tagcaaagcg cagactttta ctctcgtggc aaaagatctg tctgacgtca ccctcggtca
     5281 gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt attgataccg gtgtttgcgc
     5341 cgcatcagta cgtaagttta accaactggc aactgagatc gacaacaccg ttgtgctgtg
     5401 tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc ggcgcagaag gtctgaacaa
     5461 cgttatcacc ctctccactt tccgtaacgc tgaatttctg caagcctacg gtgtggcaat
     5521 tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt gtggttattg acgaaaatga
     5581 caatgtgatt ttcagccagc tggtggatga aatcaccacc gagccggatt acgaagcagc
     5641 tctggctgta ctgaaagcat aagttaatat gcctgatggt gttgcaccat caggcatatt
     5701 cgcgcttact cttcgttctt cttctgactc agaccatatt cccgcaactt attggcaatc
     5761 gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg tgctgggata attgcgataa
     5821 agctgggtta ataccgagcg ttcaaaacgg ctggtgattt cgtccagcga accttccatc
     5881 gcatcttcgc ccacggctac cgttgcggcg tcataatccg gcaacaaaat atcctgtgga
     5941 cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga tagcgttctt taactgccgc
     6001 acatttcccg gccacgcata acgtgtaagt acagtattca ggtcagcggc cagtttcgga
     6061 cgcggcacgc cctgctcgtc ggcaaagcgg gcgacgaaca gctcagttaa cggcataatg
     6121 tcctgcggac agtcacgtag cggcggcaga ttgagcgtca acacgttcag acgataatag
     6181 agatcttcac ggaacacgcc tttttgcacc agttcgacca gattcttctg cgtagcgcaa
     6241 atcacccgca catcgacatg cacctcatgg tcttcgccaa cccgacggaa agtgccatca
     6301 ttaagaaaac gcagtaattt cgcctgcatc cgtggtgaca tttcccctat ttcatccaac
     6361 agcaccgaac caccgttcgc ctgctcaaag aaccctttct tcccttccgg agcatgacca
     6421 aacagttcac tctcgaccgc atcttctggt atagacgcgc aattcagcgc caggtaaggt
     6481 ttgcccgctc tggggcttgc ctgatggcag gcgtaggcaa aaagatcttt acctgtacct
     6541 gtgtcacccg taatcagcag cggcgcgctt agcatcgcca gtttctgcgc ctgttcgaca
     6601 acgtgcttca ttttcgggct gacggcgaca atttggctga aggcgctgac gtcctgggcg
     6661 gcgacatttt gcaactggcg gcccatacga atcgttgatc gcaacatcac caccgcaccg
     6721 gtcaggacgt gttgatcatt ttcatcctga agataaacag gcgtaatctc catcaggaaa
     6781 ttctgcccat taataacgac atgctcgtta tgcgaatctt gcggttcgct ttccagccaa
     6841 cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat ggttgcgcag gcgatccaat
     6901 ttttgcccaa aaagctgaca gctcgccggg ttcgccatat ccactttgct tttcatatcg
     6961 acagagagca caggttcagg caacgcctcc agtaacgcgc tcaacgccag atgctcacgt
     7021 tcggaaggca tccacgggac agtacgcaca tcggtaacac ctgcaatacg gcgtatttcg
     7081 gccatcagac tgctgaaact ctcaaactcc agttcagcaa aattgaggta gattcgccca
     7141 atgggatcaa tctcaatacc gcgtaaatca atgcctctta gcacgagtag atcgagtaat
     7201 tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca gacgcatggg aaccttcacc
     7261 tgaaaaaaga acaattaata tgatgatatg acactatttg gatagcagga aggtttctgt
     7321 caacaatcat tgacacaaag acggaaaaac gggcacttcg gcgtaaagat atccggtcag
     7381 cacccgatat tgcattattt ttcgctgggc gttttgcctt tttgcagagt ttctttcacc
     7441 tgaccgataa gctgacgacg gaaatcccca aggcgaggtt tgtcatcgtc aatccacggc
     7501 agcgggcggc aaagctccat agctttaatc ccgagtcgta ccgtcagaag tccagcacca
     7561 attccctgag ctgcgcgggt agacaaacga gcagcgagat cttgcgacat ccagtccatc
     7621 cccacttcgc gcaccagttc gctggctccg gcaaaagcga tattcagcaa taccagctta
     7681 aacagacgca aacggctgta ataccccagt tcaatgccat acagcgtggc aatgcgatta
     7741 attaaacgca gattgcgcca ggcgataaac gccatatcga ccaacgccag cgggctgacc
     7801 gcaatcatca acgttgattc cgccgccgaa tggctgattt cgcgccgcgc ctgggcatct
     7861 aaaactggct ggaccaaatg cgcatacagg ctgaccactt cacgatcgtt ctgcgtttca
     7921 tggattgagg cataccagcg ttgcagcgcc ggatgcgact gatcaatacc cgcctgctgc
     7981 gccagttttt cgcaaaatgc gcgacctttg cccgtgccgt ggctatgcaa taaatcacgc
     8041 gcttcgtcgc gttcatgggc gcgctgtcgc aagcgccata agcgccgcca ctctgttacc
     8101 acagaaccta cgccagcgcc gataatcaat gccccagcgg cacatccacc cagcgctacc
     8161 cagtcctggg tttgccaggc attcattgtc cactgtatac cctgcccgac aacgcttgcg
     8221 ccaaacagag ccagcccgcc catcaccatt ttgcgccaca ggctgcgttt cggacgtaac
     8281 gctgcgtcca ttaccgcttc gacttgcccc tcttcttcct gcgcttcgtc gagcgtggcc
     8341 ggggcaaaat tttgcgcctg attttcgtca aaggtctgct gcgccctgaa ttttggattc
     8401 tgttcgacct ccagcggacc gtcgaaatca atacgtggtt ttaacggttc ggtcatcgca
     8461 atttatctcc tattaaaaat tccagcgcgg catcaagacg aatatgcggc agcggtttgt
     8521 cgacatccat cacctgcggg cgaaacgctt caaactggaa cccttgcttg tcccagaacg
     8581 cctgaccggg caaacgcgca ggaacttcac cggggtaaac ggtgagaggc gcaccatcgc
     8641 tgagccggtt accgcgcaat gccggaattt tctcgccgtt gacgtcaata atgccgctgg
     8701 tggtcgcctg caccgaggcc agccccaggc aatccatact aatcctttca aacgccgcat
     8761 tttgccaggc atcctgaatc agttgttgca gtaatgaaac catattggcg tgctgatcga
     8821 ccgttacatg gtccgctttc atcgcggcaa acagcagttt gtcgataacc ggcgagaata
     8881 agcggcgaaa cagcgtgcgc tgcccgtagt ggaaactttg catcagctgc gtcagtgcca
     8941 gacgcatatc attaaatgcc tgtggcccac tgttgagagg ttgcaggcaa tccaccagca
     9001 caatctggcg gtcaaagcgc agaaaatgat tcttatagaa ccccttcacc actttctcgc
     9061 agtaataatt aaaccgctcg cgcagcattc cggcattggt gtgcttatcg gcctgcgcca
     9121 gtttggactc gtcccaggca tcgacatccg gccacgggaa gaattgcagc gcgggcgcac
     9181 ctgccatatc tcccggcaag acaaagcgcc caggctgaat aaagtgcagc ccctgctgtt
     9241 tacagtggtg gagataatcg gtccacacgg cggcaatgtc tgccagccgg ttttcgtcgg
     9301 caggcgctag cgggtcaagt ccttcgcaca tcattcgcca tttcgccgac cattcgccgc
     9361 gctgaccatt gagtaagccc gtcatctggc gcgaccagct taagtaatcc tgcgccagca
     9421 tcggcaggtc gagcaaccat tcgccgggat aatccacaat ttccagatac agcgtggagg
     9481 tatccttaaa gtggcgcagc agcgaatcgt tcgatttaaa acgtaacgcc aggcgaattt
     9541 cactgacgcc gcgcgtaggc gtcggccagg cgggaggatc accatataac tgcgccagtc
     9601 cttcatcata ggtgaagcgc ggaatgccaa agtcacgctg gggaatgcgt tttacgccca
     9661 gcaagcgctc ttcacgcacc gcgcttaaca gcggcaaacg tgctccggca tgaatattga
     9721 gcaactgatt aaccatcgcg gtgataaacg ctgttttgcc gctgcggcta agtccggtta
     9781 cagcgaggcg cagatgtctg tcgacacccc gattcaccag cgcattgagt tcatttttaa
     9841 gtcgcttcat tgctgtcctg tgctgctctg aagaccagaa aaaaaatcat tcgtaaggat
     9901 atgaatgtgg gggccaggcc attgattatc aactgcccgt gttgtcatcg acgggcgaac
     9961 tccataagat gtgcagcatt cacgttcgga cggagttcct gacctatgtc ccctactatt
    10021 tatgatattg ccagggttgc aggcgtatca aaatccaccg tatcacgcgt gctgaataag
    10081 caaactaata tctccccgga agcgcgcgaa aaagtgttac gggccattga agaattacag
    10141 tatcagccaa acaagctggc ccgcgcgctg acctcttcgg gttttgatgc cattatggtg
    10201 atttctaccc gttcgaccaa aactacggcg ggtaatccgt ttttctctga agttttgcat
    10261 gccatcaccg ccaaagcgga agaagaaggt ttcgacgtga tattacaaac gtcgcacaat
    10321 ccggcagaag atctgcaaaa atgcgaaagc aaaattaaac agaaaatgat taaaggcatt
    10381 attatgctca gttcgccagc ggataagtca ttttttgccc aactcgataa atacgatatt
    10441 ccggtggtgg taattggcaa agttgaaggt caatatgccc atgtttattc tgtcgatacc
    10501 gataattttg gtgacagcat tgcgttgacc gatgcattaa ttgaaagtgg gcatcaaaat
    10561 attgcctgcc tgcatgctcc gcttgatgtc catgtttcgg ttgatcgcgt taatggctat
    10621 aagcaaagtc tgtccgcgca taatattgca gtgcgtgata aatggattgt tgacggcggt
    10681 tatacccatg aaacagcact gcaagcagca cggcaattat taagtcagtc accgttgcct
    10741 gaggcagtgt ttgccactga cagcctgaaa ttaatgagca tttatcgtgc ggcggcagag
    10801 aaaaatattg ctattccgca gcagttagcg gtggtgggtt atagtaatga aacgctgtcg
    10861 tttattttaa cgcctgcacc aggcggcatc gatgttccga cgcaggagtt aggtcaacaa
    10921 agctgcgagt tattattccg cttaatttcg ggaaaaccgt caccacagaa tattaccgtt
    10981 gccacgcata tgacgctgaa ataaatacca gctaccttgc ctgaatgccg gatgcgatgc
    11041 tggcgcatct tatccggcct acatttatag atcggataaa gtactcgcgc cgcatccggc
    11101 aatttagccc attatcagaa cgagtaattt acgccgacac ctgcataatg gaatttatcg
    11161 ctgtcgcctt cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac ggaaagtccg
    11221 ttctggaaat cataaccgta aaataaaccc acacggttaa aatcatggcc ttcacgttca
    11281 atatcatcct gccagtccca gttactctag cgatccagcc caatgcgcgt atacggcgtc
    11341 accgagtggt tgccgagcgt cagcggcaaa taggcgcgaa tttcttgtgt ggaaaactca
    11401 ccgttattgc ggctgtcgtc catattgaag ccgcgctcga gataatagtt cactcgcaaa
    11461 gcaaccgttt cgttgaaggt atattgcaga cctgtttccg tttcgacacg ggtatcagcg
    11521 taaccggtcg tgttcaaatc gttggcaaat ttatacatcg acaaccaacc gttgaaacgt
    11581 aaatcatcag tcagtttcac atcccagtct ggcgcgattt tccagcgctg catattcgcc
    11641 gtgtctttgc ccggttcatc aacgtagtga taaccataat tacggaaacc gccggtcagg
    11701 ccgaaactga aatcatcgct ttcgaggaac tgataatgca cctccagctc cgggcgatca
    11761 aacctcgttc cacgtttacc cgcgctataa tctaccggcc cttcctgata ataggccaga
    11821 gcaattctcc acggcccgtt ggcggcatta aaatagactg aaggctccgc cagcccatcc
    11881 atatcttcgc cataaccctc gacgttttct atttcgtaca tcgcaccgat attaaagtgc
    11941 cagtcgctct tttcctcggc ctgtgcgcag gccattcccg cacacatcac cagtgcggta
    12001 cagggtaata actttttttg ttccttgtat tatcctgggg gaatgccttc cccctgtatt
    12061 tagcgaattg ctatttccgt ttctgcatca aagaaatgac atttcgtcat atcaaaatga
    12121 atagtgatat tttctcctgc atgataatca tttaacgcac cagcacggac cactaactcg
    12181 tgcccccaac cgtggtgtag agcataaatt ccgcaccggt taattctgcc acgctaattt
    12241 tggcggaaat gttattttcc tcttgcgcgt ccggatgaat atcttccggg cgtattccca
    12301 tcacgatggg cttatgcaaa ctttcctgtg ttttcagaac cgctaatttc tcttcgagaa
    12361 tggttaattt aagcgtttcc gtaacgaatt tatcgccatc gatcgtgccg cgaataaaat
    12421 tcatcgctgg tgatccaata aatccggcaa caaacatatt cgcgggttgg ttataaacgg
    12481 ttttcggcgc acctacttgc tgaacaatcc cgtctttcat aatcacaatc cgcgccgcca
    12541 tggtcatcgc ttcggtctga tcgtgggtca cgtagatcat ggtggtgttc agtttctgat
    12601 gcagcttgct gatctctgca cgcatttgta cgcgcagctt ggcatcaagg ttagagagcg
    12661 gttcatccat taaaaacacg cccgcttcgc gcacaatcgc ccgcccgagc gccacacgct
    12721 ggcgttgccc gccggaaagc gcgcccggct tgcgtttcag gtactcacgc aggccgagaa
    12781 tttgcgccgc ccagttcacc cgctcatcaa tcacctcttt ggcgattttt tgcatcttca
    12841 ggccaaacgc catgttgtcg taaaccgtca tatgcggata caacgcgtag ttctggaaca
    12901 ccatcgcgat attgcgtgct ttggctggaa cgtcattcat tcgtttgccg tcgatcaaca
    12961 gatcgccgcc gctgatctcc tcaagcccgg caatcatgcg cagggtggtc gatttaccgc
    13021 agcccgacgg gccgacaaat acgatgaact ctttatcggc aatttccagg ttgaagtcct
    13081 tcaccacatg cacctggtta tcgtagattt tttgaatatg ttgtaacgaa agctgagcca
    13141 tgttgattcc tttgctatac gttttgccag aaggccgata accgcgacca ggtcagggag
    13201 tccgttgagg gcaacagtaa ttgcgccccg gttaagcccg caccgatccc caccgagcgc
    13261 ataccgctgg cgttaatggc gtcaatgccc gcctgcgcat cttcaatgcc gatacatgcc
    13321 tgcggcggca cgcccagccc ggcacaggca gcgagaaaga tttccggatc cggtttcgag
    13381 tttttaagtt gggaagcatc cgcgcagaag gtgaaaaact cgcgcagctc cagcgccgct
    13441 aaaatcgtcg gcgcattcag ggagacggaa gccagcccta ccggaatctg ctgtgcacgg
    13501 agctctgcca gcaaattgcg aataccgggc agcacagcat taaccgtcag ctcgcgcagt
    13561 gagtggacgt aaagcagatt tttgcgatac gccagttgcg cccgctcctg cgggttaaag
    13621 tcgccctctt tgcccccgtg ttgcagaatg cgccgcagag actcatcgcg gctgatccct
    13681 tttagagatt cgttaaactg cgcatcaata ctgatgccaa tttcagcggc aatctgctgc
    13741 cacgcctgga aatgcagatg cgcggtatcg gtgattacac catccagatc gaaaattacc
    13801 ccttgcagtt tcattcatcc tcctgatgcg tggtagcggt cccattgaag ggcaaaataa
    13861 aatcacccaa acagaaaaca gattcttcag ccacggatat aagctgaccg ttcaaacgca
    13921 gtgaaacggg cgcagaagtt cgaatcgcaa tacgctgcgc gtcgagagtg acctgtaatt
    13981 cgcagccctg ccagaacaga gggaaagaca actgttgcca ctgctcaagt aacgccggat
    14041 ttagatgcaa ttcaccgtca cgcacgctca ccccggcaaa accctgaatc gcccccagcc
    14101 agatagcgcc agttgcggca gcatggatac catcatcaca actatgcgga tcagcaccaa
    14161 gatcgatttc agtcccctcg cgccagaact gataactttg ggtcagcagg ccacagcgtg
    14221 cagcaacaat gccgtggatt gctttactta atgacgagtc gtgaatagtg cgcggttcat
    14281 aaaattgcag attggcaaga cacgatgccg atgagaactg ctctggcagc atgtaattga
    14341 gcatcaccac atcagcttgt ttgaggatct gcatctcgtt cacttctgcg cgtgaataat
    14401 ccagcagaat ggtttgcttc cccgccgccg ctttgtattt cgccagatta atcgccggct
    14461 tagtcataaa cgaatcatcc tgcggcaaaa caccgtcggg ctgaatttct ggcagccgca
    14521 gttctttaag gaacatttcg gcacgatgaa taaacgcgtc gtcactacag ccaaactggc
    14581 gggcaatact cagcgcctgc tggacgttgt aatacaccat atagctggtg aaggcgttat
    14641 tattgacatg ttcggtatat tcgtctggcc caataacatc atgaatttcc agacggtcgt
    14701 taaccctcac cgcgcggcta atccagaact ttgcagtttc cagaagtagc gccatgcctt
    14761 catgcgcaat gaaactttca tcccccgtgg tctgccagta ttgaataacc gcccaggcga
    14821 tatcggccac cagatgatgt tccgcctgcg ccgaggccac tttttgccgc agcccggtgc
    14881 gaatgttaat ggcggcaaat tccggcgtct cttcttcgcc gctgcgcgcg ctttcccacg
    14941 gaaatagcgc gccctgccag ccgttgcgtc gcgctttctc ctgcgcgcct ggcaagttgt
    15001 gccagcgata acgcagtaaa cttcgggcaa ccgtcggatc gctaaacaga tggaacggca
    15061 gcaaaaatac ttctgtatcc cagaaaacgt ggcctttgta gccttcgccg gttaagcctt
    15121 ttgccgcaat actgctgcgc tcgccgtgag ccggcgtcat gatgcgcaga tgataaagcg
    15181 cataatctaa cgcttgctga tcgtgcgcat cgccgccatt taccgtgata cgacgtttct
    15241 gccaccattg acgccagttt tctgttgatg ctgcaagatg ttggtcgtaa ctctgttgcg
    15301 cgcacatttc aagctggcga agcgacacgc tgccccactc gtctaaagca gcttgcctgt
    15361 catcccgcca gtcgatccac accagttttt gcaacgtcac tgtctcgcct gcatgaagct
    15421 gcgtactggt atgttgcagt aaacggcgct ctttggcggt atagcattgc tgcacatcac
    15481 cgctcacctg gcagcaacaa ctgatggcca catcactgcg cccatcctgg gtggtgtaga
    15541 tcccctgcat caaatgctga ccaaacaccc gcacctgggt ttcgtcgaga tgctggcgac
    15601 cgtggttggt ttgcgtggcg tcaatgcctg ttgaaatcag cactgacgcg tcggcgtcca
    15661 gtggcataat agtgatttcc agcgcaatga gcggcagttg gtctgccgaa acaaagcgac
    15721 ggctggcgat ggtgtaacct gcgccgttgc tggtacgcca gacaacgcta cggcgtaatt
    15781 ctccactggc aaagtcaagt tcacgctgcc aggcttcgcg ggataacgag aaaacctcac
    15841 catttatggc aatctccatc cccaagatat caggcagatt caccagttcg ttgatttcac
    15901 cttttcccgc ccgatgatac agccccgcca gatacatccc tcgcgtctgg cgcgtgtaat
    15961 cttcttcatg gctggcgcga agcccaagat agccgttccc ctgcgccatc agcgatgcat
    16021 acttgttcag ggtatgctgg ctgaaatggg gttctgataa cgttactggc ctggtcataa
    16081 ttccacacgc gtccctgttt ctgcggattg atataacgcc gccaccagtt gctggatgat
    16141 gtacccctgc tcggcgtcgg ctatcatcac gggcttgccc tgtacgtgat tgataaaggc
    16201 ttccatgctg cgcaaatggc ggttgtcgtc tgccatttcc cgttgcatca gcgtcattaa
    16261 ttcaccgttg ttatcggtgt agatatgtgc tggaaacagc gtcgcaccgg ctttatcacc
    16321 acaaaagctg acgttcataa tcaactgttc gcggatgttg agtgcaaatg acgtttccag
    16381 ccacagaatg ccgccgttat gaaattcaat ggtgccaaac agcgaatctt cgacgctgta
    16441 agttgccgga tcccactcgc caaattgacc acagctcttt tgcgtgccga tcttttgaaa
    16501 actatgcgca ttcacgcttt tcaccgccgg aaagcccagc acatacatcg cagcatccag
    16561 catatgaatg ccgatgtcga tcagcaggcc accaccctgc agttctttat tggtaaagac
    16621 accccagccg ggaacgccgc agcgacgcag ggcgcgggcg gtggtaacgt aaatctctcc
    16681 caaaacgccg ttggtcacct gttcacgcag ctgttgcgta tcgagcgcaa acgatggtga
    16741 aagtcgtagg ccagcacctt gcccagtttg cgcgcggtat cgcacatttc ccacgcctgt
    16801 tctgtcgtca tggcgggcgg tttttcgcac atcacatggc agcctgcttc cagtgccatc
    16861 agggtatgtt cgtaatgaaa acggttaggt gagccgacgc taaccacatc aggtttcacc
    16921 gccagcagca tggcctgcgg atcgtcccac acggatgcat tcccgtattt ttctgccagc
    16981 gcctgcgcct gggaaaggcg gctgtcacag acagccacca gttccagatc gttgcgggtg
    17041 cagtagtacg aagcatgaac cttatccgcc acctggcccg cgcctattat cgcgacccgc
    17101 agcggagagc ttgtcattgc acttttcaca aagacctctt agcaggtacg caaccaagcc
    17161 aacgaatcac ggtacgcctg ggcaggatct tctgcccgga tacgcccttc atacaccaca
    17221 taaccctgat agttatcggc gcgcagctgt tcaaacagcg cgtggaaatc gagggtgcca
    17281 ctgcccggct ggtaacgatg gttatcggca atatgcacat gaccgagcaa gtcacggttg
    17341 tcgtgcagcg cctgcgccag gttatcttct tcgatgttca tgtgatagaa atcgccgata
    17401 atctgcacat gtttaagatt gttttcgacg atgtaacgac gagcatcggc gagggtgttg
    17461 atcatatgat cctgatagcg gtttaacggt tcgagataca ccacggttcc ggtacgcgcg
    17521 gcgacctgtt ccagtacgcg cagggaatca ctcaccagtt tgcggtcgcc gtccaggcta
    17581 cgcggcgagg tcatcggcgg taagcggaag gtaaacatgc cccacgctgc cggaaacccc
    17641 ccacttctgc cagcgcctcg agaatgcgtt cgatctgctt taagccatta agacgacgct
    17701 cttcgataaa gtcaccaatc cagccgtcat agccgccgca ggcggtggtc actggtaaac
    17761 cggtttcttt gatcgccgct ttgacttctt cgatgttgtt gaccagcagt ttgccgtcaa
    17821 tctcaaaacc atcgaagccc atctctttga tataacgaaa tctctcgcgg atattttccg
    17881 gaaagaatgc ctgattctgt gtgccgattt tcattttttc attcctttga attaaaacgt
    17941 aacgcccatt ttgatgctct gttccggatg ctgatcgaca tactgcatgt agctttccgg
    18001 gctgttggca aaggtcacta ccgggtcgat taaatcttcg caattgagat aaccgttcat
    18061 cagcagttcc cagcaggttt cttcaatacg cttacggctc cagcgcggat aatccgggtt
    18121 cggttcgctg cacgcgcggg agaaaacaat tttggcgtta ttgaaatgcg cttcgcgtcc
    18181 gaggttaaaa ccttcagcaa atggtttggc aaacgcgaca taggagatgg tgccgccgta
    18241 ggccaggccg cgcagcgccg attgcagcgc gtcggcgtaa ccgctggttt cgataatcac
    18301 atcagccccc tgcttgccgg tcagcgtttt gatctctttg cctacgtcag tgccaatggg
    18361 gttaaggcag aaatcagcac cgtggcgacg ggcaatatca cagcgatggg cgataggatc
    18421 gacgccaatt accaccgatg cgccagcgcg tttcgccagt tggatggcaa tttgaccgat
    18481 cgcgccaagc cctaccacca cgacaaaatc ccctacgcgt acgttggcat cgcgcacgcc
    18541 gctcatggca aactgcgccg ggtcgtaaca gacggcgttt ttccaggagc tgccttgcgg
    18601 cattttgcgc agcttgtagt tattcactgc gttaatgatg accgtctcgg agagcgggcc
    18661 gtagccgcat acgctgtcgc ccaccgcgta gtctgtaacg tcgctgccgc actcgataat
    18721 gtcgccaacc accatgttgc caagctggaa tttgccaaac tcaatgccgc gcggcgcatc
    18781 tgccggacgc ggggtgaaca tctgccactc gccgttaaag tcttcatcga taaacgggct
    18841 ggcggcgcgg aagtcgacca cttccgttcc gtgtttcggt gcgccgaaac gcacgcggat
    18901 cttcacttca ttggctaaaa tcgcccgatc ttcatactca accagcgcag caacacgcgg
    18961 tgctgtggct actaactttt tcatgtaaaa ctccttgtaa agaaactggc cgctttagcc
    19021 cttcacgcca ccggcggtca aaccactctt aataaaacgt tccgaaagcg catacataat
    19081 caccaccggc aatgcggtca ccagcgaggc cgccatcatc cgtccccaga tgtaatcagg
    19141 cgtactgaac agcgcgttca ggcccactgg taaggtgaaa ttgctggcgc tggagaggaa
    19201 aatcgacgca aacagatagt cgttccacgc caccataaag cagtagacga acaccgatat
    19261 cagcccggac atcgccaggg gcacggtgat acggaagatg atttgcagac ggttgaggcc
    19321 atccatcatc gccgcttctt cgatctcatc agggatggtg tcgaagtaac ttttcagcat
    19381 aaacacggcg gtgggcaggg tttgcgtcac catggtaatg atcagcgcca tctcggtgtc
    19441 gtaaatacca agcgcggtga tgattttgaa cagaggcacc accagcaaaa tgccagagaa
    19501 catatacacc gtgtaaaagc tggcgttgat ggtcatccgg cctttaaagc gcaggcggga
    19561 aagcgcgtaa gctccgagaa tgccgagaaa taccgccacc acggaggaaa ccaccgacac
    19621 caccatgctg ttacggaagt agtcgacaaa cgggaaaatc atcgggttaa aaatgtcgac
    19681 gtaatgctcc agcgtccact gttgcggcag cagcgtagga tgcagcgata tcgcctcttt
    19741 cgcgcttttg aacgaggtca tcagcatcac aaaaaacgga aacagcgtga tgatgagaaa
    19801 cagcgccagc ccgcagtaaa aaccgatacg actgagcgtg cgtttatttg ttgccattga
    19861 ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag agcaccaccg
    19921 agatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag agatagacac
    19981 caagaatatc gaccttggtt gtcagcagat aaacatcagc gaacatgtag aacatccaga
    20041 tggtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc gtgacgatgc
    20101 gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc gatttgtcga
    20161 tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc cagatggcga
    20221 acagcaccac cagcacaaag ctactgcccg gattatcaaa ccacagcggt gcctgctcat
    20281 agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg ttgaacatgt
    20341 atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc accagcgaac
    20401 gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccacggcc agcccgagca
    20461 cggtgctgcc cgccaccacc agcgccgtat accagaccgt catccacagc gagtgccaga
    20521 agccgggatc ggagagaatg cgcacatagt tactcacccc aacaaacgtt gactcgatgt
    20581 tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatc ggccacgcca
    20641 ccagaccgcc cagcagtaat aagctgggcg cgagaagcag cagcgcaaac ggcatatcgg
    20701 aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt gctgttcgat
    20761 cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg ctttacccac
    20821 ggtgacgtta tgcaccatgg agctcaccac accagaaccc gtcacatcgc ccatgcgggt
    20881 aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac cgattagctg
    20941 attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg cggtagtgac
    21001 aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt cggcaatgtt
    21061 gtccgcctgc tccataaagg tgacaaattt ttctgctgct tcggtctttt gtccggcggt
    21121 aatggtcagc gaggtcaaca tgccgtagac cgcagagttt ttctcggttg gcaccacgaa
    21181 accgacgttt ttcgggtcgc cttctttaat cacagccgga aggatatagg tggagtaaat
    21241 cgccatcggc gcggtgccgt tcataaaggc gtctttcact tccatgatgt cgttagaacc
    21301 cggcatggtg ttggcagcaa ggtcgcggta ataggtcagc gcctgcatca tctctggtgt
    21361 atcaagggtg attttgcctt cggcgttaaa gacgttggcc tagttggata acgcaaactg
    21421 ggagaaggat tgttccgtca gcacgctttc tgccgtaggc agcgcaatac cgtatttttt
    21481 attcgccggg tcattcagtt tctgtgcaac gtccagcagc tgttgccagt ttttcggctc
    21541 ctccagcccc gcttttgcca gcacatcttt gcgataccag atgtcgccaa tccaggcgct
    21601 gacaggaaca ccggtccatg cgctaccatc ttcggtacgc acaatacgca gtacgccatc
    21661 gtaaaacgcg ccttcaccaa cgttgctgat gaccgtggca accgctttgc gatcgataag
    21721 ctgctcttta tccataactt tggcgtagtc atggctggtt tcgatcactt ccggcagcga
    21781 gccgctacgt gaaagggtga tgactttggt gttatgagcg tcttcttcca cgggcacttg
    21841 tttaacggtg atacctgggt tttctttttc aaaacgggca atcaatttgc tgataactgc
    21901 ctgacgctcc tgctccaccg aagaatgcat aaattcgatg gatacatggg ttttattttc
    21961 ttctttacag cctgaaatca gggcgcagga aaccagtgct gataacagca cgatttttga
    22021 tttaatcatg agatgttcct ttttatttaa tccacataac ctgccagggg cgcagcgtta
    22081 tttcattacc caatatatca acttcactaa tcagatcccg accatgcaga tcagtaatat
    22141 ttacatgctg aatattttta ctgacattaa acaatccagt cagacaatta ccatcagcgt
    22201 tacttctttg aatacgcatt acggatgaat taaccgtatc gatggtaaaa ttattatccg
    22261 gatgaaactc gttatggctg cgacgaagtg taattaaacg cgacaactca tgatataccg
    22321 catgccttaa tgtagcttca tcgttcagtt ctcgtgttat ctcttcactg tgatattttt
    22381 tacggttaat cgcacggtta tatccgagtt tttcgacacc tgcgtaatca ttacgcgagc
    22441 caagaatact ttgaatatat atcgcgggta caccggggaa acttaacaaa atcgcatggg
    22501 caaggataaa cctggtgcaa cgttcttcat cgctactctc gcggcggctt aacgcattca
    22561 tataggtgac attcatctca tagggactgc gcgtgccgtc gggattattt ttccagttta
    22621 ct
//
LOCUS       DAECNO010000063        22159 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.063, whole genome shotgun sequence.
ACCESSION   DAECNO010000063 DAECNO010000000
VERSION     DAECNO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.063"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KKO08_002531"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="KKO08_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBD6318734.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="KKO08_002532"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="KKO08_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBD6318735.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="KKO08_002533"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="KKO08_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBD6318736.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KKO08_002534"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="KKO08_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBD6318737.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="KKO08_002535"
     CDS             3251..5011
                     /locus_tag="KKO08_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBD6318738.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="KKO08_002536"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="KKO08_002536"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBD6318739.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KKO08_002537"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="KKO08_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBD6318740.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KKO08_002538"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="KKO08_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBD6318741.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KKO08_002539"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="KKO08_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBD6318742.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KKO08_002540"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="KKO08_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBD6318743.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="KKO08_002541"
     CDS             complement(9679..11586)
                     /locus_tag="KKO08_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6318744.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KKO08_002542"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="KKO08_002542"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBD6318745.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="KKO08_002543"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="KKO08_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBD6318746.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KKO08_002544"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="KKO08_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBD6318747.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KKO08_002545"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="KKO08_002545"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBD6318748.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="KKO08_002546"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="KKO08_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="KKO08_002547"
     CDS             complement(17598..18113)
                     /locus_tag="KKO08_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6318749.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="KKO08_002548"
     CDS             complement(18121..18663)
                     /locus_tag="KKO08_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6318750.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="KKO08_002549"
     CDS             complement(18761..19744)
                     /locus_tag="KKO08_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6318751.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..22107)
                     /locus_tag="KKO08_002550"
                     /pseudo
     CDS             complement(19735..22107)
                     /locus_tag="KKO08_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
BASE COUNT         5493 a         5803 c         5205 g         5658 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaacaatggt aatgactcca acttactgat agtgttttat
    22141 gttcagataa tgcccgatg
//
LOCUS       DAECNO010000064        21744 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.064, whole genome shotgun sequence.
ACCESSION   DAECNO010000064 DAECNO010000000
VERSION     DAECNO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.064"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..142)
                     /locus_tag="KKO08_002551"
     CDS             complement(<1..142)
                     /locus_tag="KKO08_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBD6318752.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LST"
     gene            complement(135..1025)
                     /locus_tag="KKO08_002552"
     CDS             complement(135..1025)
                     /locus_tag="KKO08_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBD6318753.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(1055..2311)
                     /gene="wzx"
                     /locus_tag="KKO08_002553"
     CDS             complement(1055..2311)
                     /gene="wzx"
                     /locus_tag="KKO08_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBD6318754.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(2327..2872)
                     /gene="rfbC"
                     /locus_tag="KKO08_002554"
     CDS             complement(2327..2872)
                     /gene="rfbC"
                     /locus_tag="KKO08_002554"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBD6318755.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(2877..3755)
                     /gene="rfbA"
                     /locus_tag="KKO08_002555"
     CDS             complement(2877..3755)
                     /gene="rfbA"
                     /locus_tag="KKO08_002555"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBD6318756.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(3814..4713)
                     /gene="rfbD"
                     /locus_tag="KKO08_002556"
     CDS             complement(3814..4713)
                     /gene="rfbD"
                     /locus_tag="KKO08_002556"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBD6318757.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(4713..5798)
                     /gene="rfbB"
                     /locus_tag="KKO08_002557"
     CDS             complement(4713..5798)
                     /gene="rfbB"
                     /locus_tag="KKO08_002557"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBD6318758.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(6171..7064)
                     /gene="galF"
                     /locus_tag="KKO08_002558"
     CDS             complement(6171..7064)
                     /gene="galF"
                     /locus_tag="KKO08_002558"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBD6318759.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(7239..8633)
                     /gene="wcaM"
                     /locus_tag="KKO08_002559"
     CDS             complement(7239..8633)
                     /gene="wcaM"
                     /locus_tag="KKO08_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBD6318760.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(8644..9864)
                     /gene="wcaL"
                     /locus_tag="KKO08_002560"
     CDS             complement(8644..9864)
                     /gene="wcaL"
                     /locus_tag="KKO08_002560"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBD6318761.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(9861..11141)
                     /gene="wcaK"
                     /locus_tag="KKO08_002561"
     CDS             complement(9861..11141)
                     /gene="wcaK"
                     /locus_tag="KKO08_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBD6318762.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(11321..12799)
                     /gene="wzxC"
                     /locus_tag="KKO08_002562"
     CDS             complement(11321..12799)
                     /gene="wzxC"
                     /locus_tag="KKO08_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBD6318763.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(12801..14195)
                     /gene="wcaJ"
                     /locus_tag="KKO08_002563"
     CDS             complement(12801..14195)
                     /gene="wcaJ"
                     /locus_tag="KKO08_002563"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBD6318764.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(14250..15620)
                     /gene="cpsG"
                     /locus_tag="KKO08_002564"
                     /gene_synonym="manB"
     CDS             complement(14250..15620)
                     /gene="cpsG"
                     /locus_tag="KKO08_002564"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBD6318765.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(15813..17249)
                     /gene="cpsB"
                     /locus_tag="KKO08_002565"
     CDS             complement(15813..17249)
                     /gene="cpsB"
                     /locus_tag="KKO08_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBD6318766.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(17252..18475)
                     /gene="wcaI"
                     /locus_tag="KKO08_002566"
     CDS             complement(17252..18475)
                     /gene="wcaI"
                     /locus_tag="KKO08_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBD6318767.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(18472..18951)
                     /gene="gmm"
                     /locus_tag="KKO08_002567"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(18472..18951)
                     /gene="gmm"
                     /locus_tag="KKO08_002567"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBD6318768.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(18951..19909)
                     /gene="fcl"
                     /locus_tag="KKO08_002568"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(18951..19909)
                     /gene="fcl"
                     /locus_tag="KKO08_002568"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(19912..21033)
                     /gene="gmd"
                     /locus_tag="KKO08_002569"
     CDS             complement(19912..21033)
                     /gene="gmd"
                     /locus_tag="KKO08_002569"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBD6318769.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(21059..21607)
                     /gene="wcaF"
                     /locus_tag="KKO08_002570"
     CDS             complement(21059..21607)
                     /gene="wcaF"
                     /locus_tag="KKO08_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBD6318770.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(21623..>21744)
                     /locus_tag="KKO08_002571"
     CDS             complement(21623..>21744)
                     /locus_tag="KKO08_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBD6318771.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         5429 a         5731 c         5011 g         5573 t
ORIGIN      
        1 cagttgataa atcatctgat ataaaaattg agatgtctac atctttttgt tcaataatac
       61 tttttaattg ttcctcaatc cagcattcgc cattatacgt agccataagt acagctactc
      121 ttttttgatc agaactatgc attataacga ccgcccccag taattcctct tattccatcc
      181 agcgtagcca aaatatgttt tttcatatat tcaattttat ttttttgagt tgcgataatt
      241 aaagtcgtat gaattaaaga aaaaacaact tccctaatag agagtaaaag aggcacatga
      301 ttttttctaa gcaaaagaaa tgcatttctt actcgataat aacgacgtac tggcgagtga
      361 attggtatgt taatcccaca gatttttact gacttatccc caatagcatg aaccatttct
      421 atatcaggaa tgatatgaac taatatgcca tagtttaagc atctaagaca ccattctgta
      481 tcgacataat caataaaaag ttcctcatcc atcattccaa caatctcaaa aacttctttt
      541 gaaaccatgg ttccggatga gattgttact gaacttttaa acggtgtctg tccttcagtg
      601 acatgaattt tctctcgaag accatttttt ttgatattac aaataggata ataatgtgac
      661 ttatctctat caataaaaac tgggccagct atttttgcat tttcattatc catatagcag
      721 gaaagttttt tgataaactc attgcgaata cggctatctt gatcaaaaaa aattatcttc
      781 tctgcccctt tttcaagaga atatcgaatc ccaatatttt gtgcttttgc tataccttca
      841 ttcctaccaa gacaaattaa atgaactttt tcaatgtctg ctaatttatc tttcaaggtg
      901 aaattagtcc tattatcgac tactacaacg taacaatttt gctcctttag ttcagtgatc
      961 agtgcattca gatttttaag ttcgggatta tatgtcacaa tgacagcgta aatattacta
     1021 ttcattatcc aagtgactca gtaattggtt aattttagtt ttcatataca gaacacattc
     1081 tagtataaag ccattgtaat aacagagcat ctaaaattac acgtatgctc caggatattg
     1141 cggcacccag taacccatat ttttcgacca taaaatacaa aagggctaaa taaggaacca
     1201 attcagcaca atgaatcaaa gcagtaattt tagattttcc taatgcctgg attgcagaga
     1261 atggtatcat tgccagcgca ttgaagaaaa aaccgatcaa taacacattt aatatattaa
     1321 aggaattaat tttataattc tcaccaaacc atattttcaa tacaagccct gaatataaca
     1381 agcctatgat tatcaccggc aaacagatta gaaacattaa aagcaatgat ttatttacat
     1441 tacgtttaaa ttctttaaag tctttgatgt tacttaacct tggaaacact gccctcccga
     1501 ttgctgcagg tattattcct aattttagaa taacctccgc tggtgcagaa taaaatgcaa
     1561 ctttatctgc ccccataata tttgacacta taaatcgatc aaaatacacc atgaccggac
     1621 ttatgatatt acttactgtc atccaaccgc caaagaaaaa gagtcgttta aaggttttat
     1681 aacaaaaatg aacccccgaa attttaatat cattcctgac ataataggca gagactaaaa
     1741 tcgaaataac tcttgcaaat attaaaccag caaccgctgc cgacaatgta gcactataaa
     1801 aaacaaatat ggccggaatt cccgcgatgc aagagcttga tatagatttt tgaatattta
     1861 caatgccaaa tttttcatcc ccctcaagaa tggctgacca taattgattt agaataaata
     1921 atggaatgca aatagccaac agtttaaatg ctagttgtat atcactatgt tcaacaccag
     1981 aaatttttag ataattaaca attccatcag agaaaatcaa taataaaaaa gcaccaaagc
     2041 atgaaaatag caccaaaaaa gatgtacttg ttgaaattac cttggttctt tcatgatgat
     2101 tatcacgatg aattgcaatt tctctaataa cagagcgagt caggcctaca tcaaaaattc
     2161 cagcataccc aacaagtgca attgcaattg tataaatacc gaaaccttca ggccccaaac
     2221 ctcgagcgag aaatcctagc gaggggatag caattaaagt tggtattgca tagccaaaaa
     2281 ggttccagac actatttttt attatgctca ttattacatt tctttatcat ataaaataat
     2341 cagcatctac aaatttttga ccattaatat ctttatcaga aagcaatgga ttttgaaccg
     2401 gccattctat atttatactt ttgtcgctcc ataaaatccc acgctcttgc atttttgaat
     2461 agaaattatt tgttttgtag acaaattctg cttcatcact aagaacgcaa aacccatgag
     2521 cgaatccttc cggtatccat aattgacgct tattctccgc cgacaaattt acaccaaccc
     2581 acttgccaaa tgttggtgat gatttccgaa tatcaacagc tacgtcaaaa acttcaccga
     2641 caacacagcg taccagtttt ccttgagcat aaggttccag ctgatagtgc agtccgcgta
     2701 acacaccctt agttgattta gaatggttat cctgaacaaa ctcaaccttt cgtcctacag
     2761 cctcttcgaa aactttctga ttgaagcttt ccataaaaaa gccccgctca tcaccaaata
     2821 cttttggttc aaaaattagc acatcaggaa tttcagtttt aattacgttc attttattaa
     2881 taacctttaa tcattttcag cagatactga ccataagcat ttttcttcag cggctcggct
     2941 aataccttca cttgctcagc atcaataaag cctttacggt aagcaatttc ttccgggcag
     3001 gaaactttca gcccctgacg ctcttcaatt gttgcaatga agttgcttgc ttcaataaga
     3061 ctttgatgtg tccccgtgtc cagccatgca taaccacgcc ccatcatagc gacagacaaa
     3121 cgtccttggt ccatataaat acggttaata tcggtaattt ccagttcgcc acgtgcagat
     3181 ggtttaaggt ttttcgccat ttctacaacg tcattgtcat agaaataaag cccagtaacc
     3241 gcatagttac tttttggttc cagcggtttt tcttccagac taattgccgt accgttatca
     3301 tcaaattcca ccacaccgta gcgctcagga tcatttacgt gataggcaaa aaccgttgcg
     3361 ccactctctc tgttaacagc ggtatccatt aatttcggca ggtcgtgacc atagaagata
     3421 ttatcaccaa gtaccagcgc acaatcatca ccacctataa attcttcacc aatgataaat
     3481 gcttgcgcca gaccatccgg actcggttgt actttatatt gcagattaag cccccactgg
     3541 cttccatcac caagcaattg ttgaaaacgt ggtgtatcct gcggcgtact gataatcaga
     3601 atatctcgaa tacccgccaa cataagcgtt gaaagcggat aataaatcat cggtttatca
     3661 taaatcggta gcagttgttt gctcacagcc attgtcacag gataaagacg agtgccggaa
     3721 ccaccagcca gaataatacc tttacgcgtt ttcatttcac cattcctttt aattcatctc
     3781 gctctagcat catgagcgag atgcaataat ttgttaaatt gccgtggtcg taaataattc
     3841 gttgagcata cgtttcacgc ccacctgcca gtcaggcaag acaagcgcaa agttctgctg
     3901 aaatttttcg gtattgaggc gagaattatg gggacgacgt gctggtgtgg gataggccgt
     3961 tgtcggcacg gcattaagtt tgttaagagc aaggttgatc cccgctctgc gcgcttcttc
     4021 aaataccaga gcggcataat cgtgccaggt tgttgtacca ccagcaacca gatggtacaa
     4081 accagcaact tctggtttat ttgcggccac gcgaatggca tgagcggtgc aatcagccag
     4141 taattcagcc cccgttggtg cgccgaattg atcgtttatt acagccaact cttcgcgttc
     4201 ttttgccagg cgcaacatcg ttttggcaaa gttatttcct ttaccagcgt atacccagct
     4261 ggtacggaaa ataagatgct ttgcgcaatg ttcttgtaac gctttttccc cagctagctt
     4321 ggtttcaccg tagacattta gcggtgctgt tgcatccgtt tccagccatg gcgtgtcacc
     4381 gttgcctggg aaaacataat cagtggagta gtgtataacc caggctccga cttcattagc
     4441 tgcttttgca atcgcttcga cgcatgtcgc gttaagtaat tgcgcgaaat tcggttctga
     4501 ttccgctttg tctactgcag tgtgagcagc agcattaaca ataacatcgg ggcgaatttt
     4561 tttgacggtt tcagccacac cttcggggtt gctgaaatct ccacaataat cagtggagtg
     4621 aacatcgagc gcaatcaagt tacccagagg agccagagca cgctgcagtt cccaacccac
     4681 ctgccctgtt ttgccgaaaa gcaggatatt cattactgac ggccctcata gttttgttca
     4741 atccacgact gataagcacc gcttttcaca ttctcaaccc aatttgtatt agccagatac
     4801 cattccaccg ttttacgaat cccgctctca aacgtttctt gcggtttcca gcccaattcg
     4861 cggctaattt tatcggcatc aatggcgtaa cggcggtcat gccccgggcg atcggcaaca
     4921 taagtaattt gctcacgata agatttctct ttcggtacaa tctcatccaa caaatcacaa
     4981 atagtgagca ctacgtcgat gtttttcttt tcgttatgtc caccaatgtt ataagtttca
     5041 cccgctttac cttcggttac gacggtatat aacgcacgag catgatcctc tacatacaac
     5101 cagtcgcgaa tttgattccc tttaccataa ataggtaatg ccttaccttc cagagcattc
     5161 agaataacca atggaataag tttttccggg aagtgataag gaccataatt gttcgagcaa
     5221 ttagtcacaa tggttggtaa accataagtc cgcttccagg cacgtaccaa atgatcgctg
     5281 gaagctttag acgcagaata cgggctactt ggcgcgtaag ctgtcgtttc cgtaaatagc
     5341 ggtaacgctt cgttattatt tgcttcatcc ggatggggta agtcaccata aacttcatca
     5401 gtagaaatat gatgaaagcg gaaacttttt ttcttttcat cattcagagc agaccagtaa
     5461 ttgcgcgccg cttctaaaag aacataagtc cccacaatat tggtttcaat aaatgccgca
     5521 gggcccgtta ttgaacggtc aacgtggctc tctgccgcca ggtgcatcac cgcgtctggc
     5581 tggtgctgtg cgaaaatacg cgacatggct acagcatcgc agatatctgc atgctcaaaa
     5641 gcataacgtt cagaatcaga aacatcagcg agtgattcca ggttcccggc gtatgttaat
     5701 ttatcgacat taacaacact atcttgcgta ttatttatta tgtgacgaac aacagcagaa
     5761 ccaataaacc ctgcgccacc agtaacaagt atcttcactt ttttattcca tatagctata
     5821 gagcatgctg tgaaatagac tgctccagat ttgattaata gaggtattaa tacacgctac
     5881 cgcccctggc ttaacagcta ccagtgcact gcatgcatct caacgatgtg gcgagcgtta
     5941 gccactcgcg ccaaatccga aaaattcaaa cgttaattgt cttaccaaac agccgtagaa
     6001 acaaggaaaa tcctggaaaa atttgaataa tatgagctat gtaacttctt gtttttatag
     6061 gctttataaa aacggcaacg gcaggaaaat tcaatgagca aaacttatgc cacgaatctt
     6121 cactgccgtt ataattttct tatcaaccgt tacatccggt cagattttca ttattcgctt
     6181 aacagcttct caataccttt acggaacttc gccccttctt tcaggttgcg taggccatac
     6241 ttcacaaacg cctgcatata gcccattttt ttgccgcagt cgtaactgtc gccggtcatc
     6301 agcattgcat caacggattg ttttttcgcc agctcggcaa tagcatcagt cagctgaata
     6361 cgtccccatg caccaggctg agtacgttcc agttccggcc aaatatcggc agaaagcaca
     6421 tagcgaccta cggccatgat gtctgagtcc agcgtctgcg gctgatccgg tttttcgata
     6481 aattcaacaa tgcggctgac tttaccctca cggtccagcg gctctttagt ctggatgacg
     6541 gagtattcag agaggtcacc cggcatacgt tttgccagca cctggctgcg gcccgtttcg
     6601 ttgaaacgtg caatcatggc agcaaggttg taacgtagcg ggtcggcgct ggcatcgtcg
     6661 atcacaacgt ctggcagtac cacgacaaat gggttgtcac caatggcagg tcgcgcacac
     6721 aaaatggagt ggcctaaacc taaaggttcg ccctgacgca cgttcataat gatcacgccc
     6781 ggcggacaga tggactgtac ttccgccagc agttgacgct tcacgcgctg ctcaaggagt
     6841 gattctaact cataagaggt gtcgaagtgg ttttcgaccg cgttcttgga cgcgtgagtt
     6901 accaggagga tttctttgat ccctgcagcc acaatctcgt caacaatgta ctgaatcatt
     6961 ggcttgtcga cgattggtag catctctttg ggtatcgcct tagtggcagg caacatatgc
     7021 atcccgagac ccgctacagg aataactgct tttaaattcg tcattatttc atccacctgt
     7081 aaaatggttg ctgaattata gcttgttcga tttttttcgc cagcatcaat taccctgaat
     7141 tgattactga attacttgtg atgttacgcc gcttcgttgt ggattgcagt agcattgttc
     7201 ctaagtatgg ctccattttt ccaggaatgg tcgcaaatct actccctccg ttccggcaat
     7261 ctaaagttaa tcttttccac attaacaata tggtgattaa tcctgtcgat atcgacggag
     7321 ctttgccctc tttcattcac cgcatgaaca tttgcaagag acagcagtgt ttcttttttc
     7381 gccataaaga cgccgcgaac gtctttgcgc atgtcgaagt tcatgctcaa tgctggtcca
     7441 actgaggatt cctgcatcac attgatattt cgcataaaaa gatgttgcgg cttgttgtgt
     7501 aactccagcg aggcacgctt catctcaatg ttagttagcg acacaaagga gacggcattc
     7561 ccggcggaga tttggatgcc gcgcaattta taagcaagat gggtattatc cagttgaata
     7621 ttattcactc ggaaattttg cggtatcgag agatatttgc ctttaattac cccatagccg
     7681 attaacatcc cggcgctatt aatcatttca atattatcaa tcacgaaatt gtcacaaccg
     7741 taaatagcga ctgtcgcgtt atcaatgccc gctttcttac tgaaatccgg cgtgatattg
     7801 cgggcattga tattacgaat aacaaaatgt ttaccatttt caacatggat caactgccga
     7861 caatccgatc ccgtgatatt cgccacgaca aagtttttca ctgcctggtc ttccgggtag
     7921 ttgttatcat aagtgcttcc cgcaaggcct atgccgatgc cccagttgat tttgccgttg
     7981 gtacagttga tacgctcgat gacatggtca gatatcaaaa tatcactgtc gttaattgcc
     8041 acgttccatt caatggcgtc gccctgtaag tcgctgaact tacagttggt aatgttggca
     8101 ccgataatct ggttatgaaa tccctggcgt aagatggcgt aattagcgtg gctgacagtg
     8161 aggttatcga tggtcaggtt gcgcatgacc cgtttgtttt tgccgccaat ataaatctgc
     8221 attaccgggc caaagccgct catcgccagc cctttgatgg tgcagtcaga acaacgcaca
     8281 tccagggtaa tgttatgcat accgccgccc tctccccctg tcacctggct gccgtcctgt
     8341 aagacaaatc gccctctgcc gttgccgcgc aggcttccaa ggatgtgtaa cgttttgccg
     8401 ggagggatga agatgccggt gttgatattg tcacaaacga atccggcagg cacgacgacc
     8461 gtttgccctt cgctgaaggc ttgtttaaat gaggcgatcc agtcatgtgg gttgtagtcg
     8521 ttaatgttaa cgctttgtcg ggcgggaagt gcgcgagcga aaggggtatg gaggaaggca
     8581 agcgccgagc ttgccgtcag gaaggtgcgt cgggagagtg ttttaaatgg catacgttct
     8641 cctctataaa gcctgcagca agctggcgag ttctcgatta atcacctgct ggttaaaatc
     8701 gtgttcgact ttttcgcgcg cacgtttgac gaccggagcc agttcgtcgg tatccagttg
     8761 gctaaacgcc gccagacgtt gtgccagtgc gcgagcatcg ttctcaggca gcagccagcc
     8821 ggatttatcg gcctccacca gttcaggaat tccgctatgc agagtagaaa ccaccggaat
     8881 accgaccgcc atcgcttcca ttagcgccac cggaatgcct tccatatcgc catccgcgcc
     8941 cgttaccgat ggcaacagga agacatccgc gtcgtcgagc atctccttca cttcatggct
     9001 cggtttaaag cccggcatct ctaccacatc ttccagttga tattgttcga tgagggtgcg
     9061 caggcgtcgt tcccacaggc caatgccgag gatgcgatag cgaaatgcca cgccctgctc
     9121 tttcaactga cggcaggctt caatcgccac atgcaggcct tttttctcgg ttaagcgtgc
     9181 gacagatatg atttccagcg gcgttgcggg cgctttcacc ggacgcgggc taaagcgcgt
     9241 catgtccacg cccatacgcg atacggcgat tttttctctg gggcagccca ttttttgcag
     9301 ccttccggcc cacagatcgc ttatcggtaa catcaggtcg ccacggcgaa acagttgttg
     9361 atattcggga gtgtagtggt tgagcacttc ccgactggag atatcaatac cgtggaagat
     9421 ggtggcaatt ttgccgcgaa tgatacccag ttcgcgtagt tttgctgcgg ttacccccgc
     9481 tgggccaaaa tgagcgatga acacatcggc gcgaaacggt gttgcgacct gaccacaaat
     9541 ggcagacaaa atcaggttcc gcgactcggc accatagcgt ttgaggttga gcgcatgcca
     9601 ggtattttta cgatgaatgc cacgtaaggt ctggctggcg cggtggcgca gtttcgctac
     9661 tttgcccgca ggttcgtcct gtaaccagcg ggttctggcg acaaggttgt atttcgtcca
     9721 tgccgcgtgg gtgttctctg tatcgccttt ttgcaaagcg acaatctcca cttcaaagcc
     9781 catatcaata aacgcggtaa tttgattgag gacgaaggtt tccgacgaca gcgggaattt
     9841 caataagaag aagccgacct tcatttcacc tccccgatgc gctcaagcac agactgcacc
     9901 atctgcattc ccgtctgacg ttcgcgactg acggcttcgt taaatcgagc gttcagcacc
     9961 ggaagctggc ctaaggtatc cgcaaccatc gcttgcaggc tgccgtccag taaatgacga
    10021 atatcaatcg ccatctctgg cagtcccagc tgctgcataa tcccggcgga tttatgttca
    10081 tagttgatgg caatcgccgg agtagcaaag ttcattgaga taatggcgga gtgcaggcgc
    10141 gtaccgacgg tgagttcaca ggctccgaga attttgccca tttccagatc gttgagttca
    10201 tccatcacta cgtggtaacg ggcaggatcg ctgatgtgct ggcgcagatt gagcgccacc
    10261 atgcggtcgt ctttgttata gctgtcgata cccgtacagg tggagagcgc aatcacctga
    10321 tagccttcat cgagaatgcg attgaccacc ccggcaaagg ctttttcata cgcttgttga
    10381 gtggtgccga gacgtttgtc gaacggtgcc agttcgcgca gggtaattgc cactgttttc
    10441 tgttgtgcgg caacgtccag ccagtgttga acggcatagc tggcggtgaa gtcttcggtg
    10501 tggtgatcga ccagccacgc ggtatcgacg ccatgttcca cttttgcggt ggtgatattt
    10561 ctgcgtttca tcaaatcaag gctgaccgat tcgcgcagga tcagcgcgtc gcagtgacca
    10621 aagacatagt tcgccagttg gttaaattgc tcatcctgga aggggccgac gctgtgacca
    10681 atcataaaca gcggcttttt cgccataaat gtgcaaagcg catgttcaaa ctgcggcacg
    10741 ccgtagagat cgacaaaaaa cgatccgccg acctgaataa tggcgtcgta acctgacagc
    10801 aggcgcacga agtcggtgaa tccctgggcg atggcgatat tgcgcagctt gccagtgtcg
    10861 gtgacgcgtg agagcaatac ctgatgctgg tagcggcgac ggaggacttt tttaatgcgc
    10921 ccgacaacgc ccgccgcgct attgtgttgt gtcatctgca ggaacagcgg atcgcccatt
    10981 accgggcggt tgagcagcca ggaagaactg accggatagc ggctcatcac gtccacttcg
    11041 gtatgtggat tgaggatgtt gatggcatca agtaagccgc gcaagatggc gctgtcgcca
    11101 cgattgccgc aagtgtggtt gcccagaata agtaatttca ttttttcctc ataaatttga
    11161 tgccaggtga ggctgtgttt gttgtgttcg ttgccgtttt ttgcctgatg cgacgctggc
    11221 gcgtcttatc aggcctacag gtcccgagca cagaaccgta ggacggataa ggcgttttac
    11281 gccgcatccg gcaaccgttg tcggaaccga aaacagcaat tcaccccgcc cgtaaaagca
    11341 ttttcatttt ttcgctgcga caaaactgac gcttcacttc caccaccagc ggatggcgtg
    11401 acagcacaat catcaccaca aacgccagca cgcccgcggc tatttgcacc gccagcagca
    11461 tccccagcgc cagttgccct ttcagcacaa tgcccagcgc ataactgacc accagcgtcg
    11521 gcaacgagag ataaaacggc agccacaaac tcaggatgta ctggcgataa ctggaaccaa
    11581 gaaccggttt aatcatcacg aagtaactca gaatggtgtt gataatctgc accagcagga
    11641 aaccgagcgt gacgccgatt gcgcccgcca tctgcccacc aataacaatc gccggaataa
    11701 acagaaatgt tttgaatacg ttgaatttaa agctgatatc gacccgcgct ttcgccatca
    11761 gcagcgaacc aatcgggtta cccacggagc gcagcagacc cacaatacac agcaattgca
    11821 gcaccggaat aatgctgttc cacttttcgc cgaataccag cggtacgaag ttattcgaca
    11881 ccaccatcag ccccagcagc gccggaaagt tgataatccc cactatcgac agcagcttgt
    11941 agaagttaac acgcagcttt tcggtgtcgt cctgaatttt ggcaaacgcc ggaaacaaca
    12001 cgcgggtgat gattgggttc agcttcatcg gcggtacgac ggccacgttg tacgccaggt
    12061 tgtatccccc tgccacgccc gcgccaagaa tacgcgccag caccagcgtt gaaaggttgg
    12121 tgttgagata gttgatgatg ctgtccgccg tcagccaggc accaaaacgt aagttcggtg
    12181 ccacagacgc cagcgagaag tgcagaccgg ggcaataaat tttgcgtcca aagtagccaa
    12241 acagcagtgt tctcaccgca ctattgacca gataaccgag gatcgcggtc atcgccagcg
    12301 gccagaaatg ggcgctaact accgtaaaag tgaagcccgc cagcaccgcg ctggtttcga
    12361 tcatgccgat tttgttgaac tccagctcct tctgcatcaa cgcgcggaac tgttgcccgt
    12421 gggggatcac cacaaacgcc agcgacaatg ttttaatcaa cggtgccaag tccgggttat
    12481 tcagcacgtc gccgatgaca tcactcaaca aaaacaccgc cacacatacc acaagcccca
    12541 gcccgacgtt cagccagtac agcgtggtga gttcaaggtg actgatttct tttcgctgaa
    12601 taatcgagtt agcgataccg aagtcagaaa gcgtatctgc cagcgcgata atcaccagcg
    12661 acacagtcag caggccgaac tggtggttgt cgataatccg cgccagcacg gtcatctgca
    12721 ccagcccgag gccgatgatg atcaccgtgg caatagccga ccacttcgcg ccgctgatgg
    12781 ttttttcacg taagctcata tcaatatgcc gctttgttaa cgaagccctt gaataccgtc
    12841 aggaaaacga ttttgatatc gaaccagacg ctccattcgc ggatgtactc aaggtcgaac
    12901 tcgacgcgtt tttccatttt ctccagcgtg tcggtttcgc cgcgccagcc attaatctgc
    12961 gcccagccgg taatgcccgg tttcacttta tggcgcagca tgtagccttc aatgagctgg
    13021 cgatactgtt cgttatgcgc tactgcgtgc ggacgtggac cgacaatcga catccccccg
    13081 gtcagcacat tgataaactg cggcaattcg tccagcgagg tgcggcgcag aaagttcccc
    13141 actttagtga cgcgcggatc gttctgcgtc gcctgggtca ccactttgtc gttctccatc
    13201 actttcatgg aacggaactt ccacactttg atcggcttgc catccatacc atagcgagtc
    13261 tggcggaaaa taaccggccc tggtgaactg agtttcaccg ccagcgcaat acagcacagc
    13321 accggggaga tcagcagcag aataagcgtc gccagcacaa tgtcttccgc acgttttagc
    13381 aggcggttaa cccccgaaag cggcgtgtca tacagcggca ccaccggaac gccgttcatc
    13441 tcttcgagac gtgaatggag aatgttgaag gtaaagacgt cggggatcag cagcaccgaa
    13501 caggtggtat ccgccagttg atggaccagt tttttcactc gtgcgccgtc gcacatttgc
    13561 atcgcgatat agacgttatg aatcttgccc gcttttgcat cctcgaccag ctgttgcagg
    13621 ttacccgccc agtcgttaga aacgccgccc ggtttcgggt cgtggtaaac gcccaccact
    13681 tcaaacccta accacggctg attacggaaa ctctccatca gcatttgtcc ggcggctaaa
    13741 tcccccacta cggcgaccat ccgcttgttg tagccatgat tacgcagcca gcccgcccca
    13801 atgcgaatac atgaacggca aaccaccagt ccgatgctgg tcagcccata ccacgccagc
    13861 cagattttca gttgcgtgtc gaaatcattg ttgaacgcca ccagtccggc gctgaaaatc
    13921 acgcttaagg tccagttttg tagcaacagg gcaaattctg tcgctgcccg aacaccgcgc
    13981 catgagcgat aaaaatcggt gatgccgccc agcatctgga acaccaccag cgtaatcagc
    14041 gccaccaaca ggtgcatgta gaggaatgac agtccgctga cttcgcaaac cagccatagc
    14101 ccggcaaaca tgatggtgat atctgaaaag cgttgcacca tagagattaa cgatgcattg
    14161 gttttcgctc gctcgcgctt ttttagattt gtcatcgttg ttcctgttat tagcccctta
    14221 cccgcagtgg ggtaagggaa gatccgacat tactcgttca gcaacgtcag cagagttcgc
    14281 gttcgcgctt ccatcagcgg cacatcaccg cgcgattcca cattcaggcg caccaccggt
    14341 tcggtattgg aggtgcgcag gttaaagcac cagtcggcaa aggtcatgct gatgccatcg
    14401 ctgcgatcca ccgccagcgc ctcacggcta aaatgctgtt ccacgcggtt aatcgcctca
    14461 acgggttgcg ccagtttgct gttgatctca ccgcttgccg gaaacgccgc catccggtcg
    14521 cgcaccagtt cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac
    14581 gggatcatgc cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttcg
    14641 ccaccgtaga tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg tccggttttc
    14701 gacattaccg gcgtgccgcc tgcagcagtc accacatcaa tagtgttcca agagagacgc
    14761 ggatcatgga tgatcttcgc gccgggattt ttttcgagga acgcttctgc cagcaggccg
    14821 acaatgtagt agccctcgat aaactgccct ttttcgtcaa acagaaaaca gcggtcaaaa
    14881 tcgccatcaa aggcaatgcc catatccgcg ccgtgtttga tgaccgcatt gcgggtgtcg
    14941 tcgcgacatt ccggcagcag cgggttagga ataccgttgg ggaaattgcc gtccggtgtg
    15001 ttgtgtactt tgattaattc cacgggcgcg ccgagggctt taaagcgggc ttcaatggcg
    15061 tccaccaccg gacccgctgc gccattcccg gagttgatca ccagcttgag cggcgtgaga
    15121 tttttgacat tgatataacc gaacaggtga tcaacgtaag cgtcacgcag gttgatttgc
    15181 tgatagcgac cgcgtttggt ttcatcgacg ggaggaaagt cgttggcttc agccagacgc
    15241 tggacgtcgc gcagtccggt atcaccgctg atcgtgcgag ccccctcgcg caccagcttc
    15301 atgccgttgt aatccatcgg attatggctg gcagtaactt caatgccgcc atccacgccg
    15361 agatggaacg tggcgaaata gatctcttcg gtgccggaca taccgatatc cagcacatcg
    15421 acgcccgcat cctgtaaacc ttttgccagc gccagtttta aggtttcgct ggtgagacgg
    15481 acatcaccgc ctaacacaat ggttttcggt ttgagaaatt cgccataggc gcgaccaatg
    15541 cgccaggcga tatcttcatt cagttcttcg cctaatttcc cgcgaatatc ataggcttta
    15601 aagcaggtta attttttcat gtcgttaccc tttttcaggc aatagttggc cctgaccgaa
    15661 acggccaatt ttatttttgt cgttatttac accgcggttt cgcattcatt gcctgatgcg
    15721 acgctgccgc gtcttatcag gcctacaaaa ccgaaccgta ggtcggataa ggcgttcacg
    15781 ccgcatccga caaacagcgc ctgatgcgac gtttacaccc gtccgtagcg atccgcgaag
    15841 cgcaccacat catcctcttc gagataagag ccggagcgca cttcaattaa atcgagcgga
    15901 attttccccg ggttttccag gcagtgcgtc gcccccagcg gaatataaat ggactcgttt
    15961 tcaccaagca gtttgatatc accatcaatg gtgacttttg ccgttcccgc gaccactacc
    16021 cagtgttccg cgcggtgatg gtgcatctgt accgacaaac cctcgcccgg tttcacggtg
    16081 atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt catatttgcc ccacggacga
    16141 tacacttcgc gatgcacccg atgctcatgg cgaccatcgg ctttgatctg ctcgaccact
    16201 tttttcacat cctgtaccgc gttacggtcg gcaatcagca ccgcatcttt ggtctgcacc
    16261 actaccagat ctttcacccc gacggtggtg accaggcctg attcggcata cacatagctg
    16321 ttttcagttt tgtgattaat cacatcgccg tggcaaacgt tgccctcggc ggtgtgggcg
    16381 ctgatctccc ataatgaaga ccaggaacca acatcgctcc agcccgcatc catcggcacc
    16441 acaacagcat ctgccgtagg ttccatgacc gcgtaatcca ccgactcttc cgggcaggcg
    16501 agaaacgctt cttcatccac acgaataaaa tcgagatccg gatcgacggc gctcatcgct
    16561 ttttcgcagg catcgaggat atccgggcga tattttttca gttcttcgag atagcgtccg
    16621 gcgcggaaca ggaacatgcc gctgttccag taatattcgc cgcttgctac ataggcctgg
    16681 gcggtttcca gattcggttt ttcgacaaac tgcgccactt caaaggccac cgtatcctgc
    16741 tcacccacag acacttcacc gcgacgaata tagccataac cggtttctgg tagatccggc
    16801 acaatgccaa aggtcaccag cttgcccgct tcggcataag gcatggcatt acgcacggcg
    16861 gcacggaacg cgtcttcatc ggcaatcaca tgatccgccg ccaataccag cattaacggg
    16921 tcgctctccg ggctatgacg ttttgccgcc agcgccgcca gtgcaatggc aggtgcagtg
    16981 ttacgccctg ccggttcgag aataatgttc tcggtgagtt tgttcagttg acgcagctgt
    17041 tccgcgacaa taaagcggtg ctgctcattg caaatcacca ccgggctttc gcactccacg
    17101 ccgttcaggc ggcagatggt ggtttgcagc atggtgagat cgcctttcag gcataaaaac
    17161 tgcttggggt aaagtacgcg ggaaagcggc cataaacggc taccggagcc acctgccatc
    17221 acaactggat agagtttcga ctgcgccata attatccccg aatatcattt ataaattgac
    17281 gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc acgtgccacc gtgttatgtt
    17341 tgggcagcag gagcgcctga cgaatccccg ccaccagcgc ctcgaccgat tccggttcta
    17401 cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc cagttctgtg tgggcctctg
    17461 cggtaatcac cgcgttaccg cctaccgcca gaatattggt cagtttcgac ggcaatacgg
    17521 catctgccgc gccgcgtttt tgcaccacca gatggcaatc gcccatcttc agcagtgcag
    17581 gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt gcgcagtcca cgctgctgcg
    17641 ccattttttc cagccgcgct ttgccgccgc tctgcccgac aatggcaaaa atcagcggtt
    17701 catcgcgcag gagatcggca gcttcaataa cgttttccag cccctgcttt tcaccaatat
    17761 tgccggagta aagaatgatt tttttgttat ccggcaggcc aagctggtta cgaagggcat
    17821 caacatcggc gtctgcaaca tgctgaaaac gggcaatttc cgaccagttg gggaagaaga
    17881 tgacgttatc cgccgccacg cctttttcga tggctttatt catcatcgaa cgcgaaatcg
    17941 tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa cgccgttgcc agctgtgcca
    18001 ctttgccgcc tttgcctttt ccggcaaggc ccagccccag catggcgtcc acttcgtaat
    18061 cctgaatatg cagcacggta cgcgcaccag agagcttcgc cagcaggcgc attcccggcg
    18121 tgcaaaacag cgttggcact acgccaataa tgcgatccgg cttccagcga cgttgcgcca
    18181 tcagtggaaa gaaactgctg acggcaaaac tgccgagatg caacaagcgt ttcagggtgc
    18241 tcggctgttt tggcacgtac agcgggcagc gccacaccgt ggcagcccct tcttctcgtt
    18301 tgtagcgcca ggcggaatag ttctcaccca cctgccattg cgggtagtaa ggcggtgcgg
    18361 taatgacccg cacctcatga ccttgtgccg ccaaccattc caccatctcg ccggtgtatt
    18421 tgccgatgcc ggttaactcc ggcgagtagt taatgccgta gaccagtatt ttcataatcc
    18481 gggtactccg gcacgcttct cagcgagaaa ataggctcgg ctgttagcgt gaacatcgtt
    18541 actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg tcatgctgct catccggcag
    18601 taacagctct tcttccgcta ctctgaagcg aaaaccgagc accacatagt gagtggtgaa
    18661 atccgtgccg gagaagttat cgtcataaaa atgctgccag acaccgtaaa actggcctgc
    18721 tgttatcggc agacgcagcc ccagttccgc catcgtcagc cgctcaaatg cggcttccag
    18781 cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag taaccctgcg ccgggcggtt
    18841 ggttcttttg ccaagcagaa actcgccgcg actgttctcg acaataaagt cgagagagac
    18901 aagcggtgtg gagcgcacta cagtggcaaa gtcttcctga cgtaaaaaca tcattccccc
    18961 cgaaagcggt cttgattctc aaggaaccac tggtaagtgc tggcaagccc cgcttccagt
    19021 gagatttcgt gataccagcc aagctgatgc aggcgcgtca catccagcag tttgcgcggc
    19081 gtaccatccg gtttgctggc atcaaaaacc acccgacctt tgtaacccac cactttggcg
    19141 atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg tgccgacgtt aatgtgcgac
    19201 agcatcggtt gggtattctc cagccagact tcatgcgcca gctccatgac atgaatgctc
    19261 gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg gtgtaccgct gccccatacc
    19321 accacgtccg tgccgtcgcc tcgtggaagc gacgcagcaa tgctggtatc acatgggagt
    19381 tactcgggtg gaagttgtca tgcggcccgt acaggttggt cggcatgaca gtgcggtaat
    19441 cgcgtccata ctggcggttg taagattcgc acagtttgat cccggcgatt ttggcaatgg
    19501 cgtaaggctc gttggtcggc tccagcgtgc cctgcaataa ctcgctttct gccatcggct
    19561 gttttgccag tttcgggtag atgcaggacg atccgagaaa catcagtttg ttcacgtcgt
    19621 tctgatgcgc ggcgtgaatg atgttgctct caatcatcat gttctggtag atgaaatccg
    19681 ccggataggt gttgttggca acaatgccgc ccactttcgc cgccgccaga tagacctggt
    19741 caatgcgttc gctggcaaag aaatcatgca cggcgcggct gtccagcagg ttcagttcat
    19801 cgcgggtacg taataccagc tccacatcac cgcgctgttc gagctgccgc ttgatggcag
    19861 aaccgaccat cccacaatgg ccagcgataa aaatgcgttg tttactcatg cttatgactc
    19921 cagcgcgatc gccacgtcgt agccgtgaga tttcagcaga gagtgttttt tcgccgcttc
    19981 gaggtcatta gccaccattt cagacaccat ctctctgagg gtgatttccg gtttccagcc
    20041 cagtttttcg tgcgctttgg tcgggtcgcc gagcagcgtt tcaacttctg ccggacggaa
    20101 gtaacgcgga tcaacggcga taatcacatc acccggttta acgcccggcg cgtcatgccc
    20161 ggtgacggaa accacaatgc ccttctcttc aacacccgtg ccttcaaagc gcagtttgat
    20221 gcccagctgt gctgccgcca tttccacaaa ctgacgtacg gagtactgaa cgccggtcgc
    20281 gataacgaaa tcttccggct gttcctgttg cagcatcatc cactgcattt ttacgtagtc
    20341 tttggcgtgg ccccagtcac gcagggaatc catattgccg aggtacaggc acgactccag
    20401 cccctgggcg atgttggcga ttgcgcgggt gattttgcgg gtaacgaagg tttcgccgcg
    20461 gcgcggggat tcatggttga agagaattcc gttacaagcg tacatgccgt aggattcgcg
    20521 gtagttaacg gtgatccagt aggcgtacag tttggcgacc gcatacggag atcgcgggta
    20581 gaacggcgtg gtttctttct gcggaatttc ctgcaccaga ccatacagtt cagaggtgga
    20641 agcctgatag aaacgagttt tcttttccag accgaggaag cggatcgcct ccagcaggcg
    20701 cagcgtaccc atcgcgtcga cgtcagcggt atattctggt gactcaaaag agaccgcaac
    20761 gtggctcatt gcgcctaggt tgtacacttc atccggctgt acttcacgca aaatgcgcgt
    20821 caggttagag gtatcactca ggtcgccata atgcagatgg aatttcgggt tgcaggtgtg
    20881 cggatcctga taaatgtgat ccacgcgctc ggtgttgaat gacgatgcgc gacgcttaat
    20941 accatgcacc tcgtaacctt tttccagcag aaactctgcc aggtaagaac cgtcttgtcc
    21001 ggttacaccg gtgatgagag cgacttttga catgtcttat tcctctgtat ttttgaattt
    21061 attcagtttc aacgcgttcg cgtatcacca ctgcgggatt cccccggcaa accacatttg
    21121 ccggaagcga tttaaaaaca ctgcttcgtg cacccacgac ggtgccgtcg ccgattgtga
    21181 cgcctggggc aacaaagaca tcggttgcca gccagcattt ctcgccaatc acaataggcg
    21241 tggcgttaat ggtgaaatgt tgacttgcat ggtcgtggct accggtgcat aaataacttt
    21301 tttgcgatat caccgaatgt gcgccaatgg ttatttcacc gagggtatat aaattgacgt
    21361 catcgccaac ccacgcgtaa tcacctaagg ttaatttcca cggataggta atttttactg
    21421 acggacgaat aactacgttt tttcctattt ttgctccgaa taaacgtaat aaaaaagccc
    21481 gccagcgata caatacttgt ggcgaccagg caaatattgt tgcctgtact gcccaccata
    21541 attgcacttt aatagcgttg ccgccccgga accctttcgg caccgagaaa ccgcttaaat
    21601 cttgcattgg ttttccttat attcagactt tgttatataa ggctttagtc ttacctgtcg
    21661 tacgttggcg taaatgccag gataattctg cccagaagcc aggtacatgt aatatctgcc
    21721 gttggacttt tttggcgtca gcac
//
LOCUS       DAECNO010000065        21277 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.065, whole genome shotgun sequence.
ACCESSION   DAECNO010000065 DAECNO010000000
VERSION     DAECNO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.065"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..446)
                     /locus_tag="KKO08_002572"
     CDS             complement(<1..446)
                     /locus_tag="KKO08_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBD6318772.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KKO08_002573"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="KKO08_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBD6318773.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KKO08_002574"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="KKO08_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBD6318774.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KKO08_002575"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="KKO08_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBD6318775.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="KKO08_002576"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="KKO08_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBD6318776.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="KKO08_002577"
     CDS             complement(5216..6814)
                     /locus_tag="KKO08_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD6318777.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KKO08_002578"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="KKO08_002578"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD6318778.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="KKO08_002579"
     CDS             complement(8493..9071)
                     /locus_tag="KKO08_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBD6318779.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KKO08_002580"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="KKO08_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBD6318780.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="KKO08_002581"
     CDS             10510..10689
                     /locus_tag="KKO08_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBD6318781.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="KKO08_002582"
     CDS             complement(10809..11168)
                     /locus_tag="KKO08_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBD6318782.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="KKO08_002583"
     CDS             complement(11530..11874)
                     /locus_tag="KKO08_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD6318783.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="KKO08_002584"
     CDS             12006..13916
                     /locus_tag="KKO08_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBD6318784.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="KKO08_002585"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="KKO08_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBD6318785.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="KKO08_002586"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="KKO08_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBD6318786.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15495..17180
                     /gene="fadD"
                     /locus_tag="KKO08_002587"
     CDS             15495..17180
                     /gene="fadD"
                     /locus_tag="KKO08_002587"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBD6318787.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17262..18377
                     /gene="rnd"
                     /locus_tag="KKO08_002588"
     CDS             17262..18377
                     /gene="rnd"
                     /locus_tag="KKO08_002588"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBD6318788.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="KKO08_002589"
     CDS             complement(18431..19396)
                     /gene="yeaX"
                     /locus_tag="KKO08_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBD6318789.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19452..20069)
                     /gene="yeaW"
                     /locus_tag="KKO08_002590"
                     /pseudo
     CDS             complement(19452..20069)
                     /gene="yeaW"
                     /locus_tag="KKO08_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20411..20977)
                     /locus_tag="KKO08_002591"
     CDS             complement(20411..20977)
                     /locus_tag="KKO08_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6318790.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21189..>21277
                     /locus_tag="KKO08_002592"
     CDS             21189..>21277
                     /locus_tag="KKO08_002592"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6318791.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5269 a         5274 c         5466 g         5268 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggca ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt attgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggt tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgggtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaacagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga cgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt cccacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaaaa agaaacagcg gctggtccgc tgtttctgca ttcttacggt aaagataaaa
    15361 ataaatagtg acgcgcttcg caaccttttc gttgggtaat tatcaagctg gtatgatgag
    15421 ttaatattat gttaacagcg tgtatatcat ttggggttgc gatgacgacg aacacgcatt
    15481 ttagaggtga agaattgaag aaggtttggc ttaaccgtta tcccgcggac gttccgacgg
    15541 agatcaaccc tgaccgttat caatctctgg tagatatgtt tgagcagtcg gtcgcgcgct
    15601 acgccgatca gcctgcgttt gtgaatatgg gggaggtgat gaccttccgc aagctggaag
    15661 aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt ggggctgaag aaaggcgatc
    15721 gcgttgcgtt gatgatgcct aacttattgc aatatccggt ggcgctattt ggcattttgc
    15781 gtgccgggat aatcgtcgta aacgttaacc cgttgtatac cccgcgtgag cttgagcatc
    15841 agcttaacga tagcggcgca tcggcgattg ttatcgtgtc taactttgcc cacgctctgg
    15901 aaaaagtggt tgataaaacc gccgttcagc acgtaattct gacccgtatg ggcgatcagc
    15961 tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa atacatcaag cgcttggtgc
    16021 cgaaatacca tctgccagat gccatttcat ttcgtagcgc actgcataac ggctaccgga
    16081 tgcagtacgt caaacccgaa ctggtgccgg aagatttagc cttcctgcaa tacaccggcg
    16141 gcaccacagg tgtggcgaaa ggcgcgatgc tgactcaccg caatatgctg gcgaacctgg
    16201 aacaggtcaa cgccacctat gggccgttgc tgcatccagg taaagaactg gtagtaacgg
    16261 cactgccgct gtatcacatt tttgccctga ccattaactg cctgctgttt atcgaactgg
    16321 gtgggcagaa cctgcttatc actaacccgc gtgatattcc agggttggta aaagagttag
    16381 cgaaatatcc gtttaccgct atcactggcg ttaacacctt gttcaatgcg ttgctgaaca
    16441 ataaagagtt ccagcagctg gatttctcca gtctgcatct ttccgcaggc ggtgggatgc
    16501 ctgttcagca agtggtggca gaacgctggg tgaaactgac cggacagtat ctgctggaag
    16561 gttatggcct gaccgagtgc gcgccgctgg tgagcgttaa cccgtatgat attgattatc
    16621 atagcggtag catcggtttg ccggtgccat cgacggaagc caaactggtg gatgacgacg
    16681 acaacgaagt accaccgggt caaccgggtg agctttgtgt caaaggaccg caggtgatgc
    16741 tgggttactg gcagcgtccg gatgctacag atgagatcat caaaaatggc tggttacaca
    16801 ccggcgacat cgcggtgatg gatgaagaag gattcctgcg cattgtcgat cgtaaaaaag
    16861 acatgatttt ggtttccggt tttaacgtct atcccaacga gattgaagat gtcgtcatgc
    16921 agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc ttccggctcc agtggtgaag
    16981 cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac cgaagagtca ctggtgactt
    17041 tttgccgccg tcagctcacg ggatacaaag taccgaagct ggtggagttt cgtgatgagt
    17101 taccgaaatc taacgtcgga aaaattttgc gacgagaatt acgtgacgaa gcgcgcggca
    17161 aagtggacaa taaagcctga gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt
    17221 tttttgacgc ccactaaaga gaaaacaatt tgaattacca aatgattacc acggacgatg
    17281 cgctggcttc tttgtgtgaa gccgtccgtg tctttccggc gatagccctg gatactgaat
    17341 ttgttcgtac gcgcacttat tacccgcagc tggggttgat tcaacttttc gatggcgagc
    17401 atctggcgct aattgatcct ctcgggatca ccgactggtc accgctgaaa gcgatcctgc
    17461 gcgatccgtc catcacaaaa tttctccatg caggcagtga agatctggaa gtgttcctca
    17521 atgtctttgg cgaattacca caacccttga ttgacacgca aatccttgct gccttctgcg
    17581 gacgcccgat gtcatggggt ttcgcttcca tggtggaaga gtattccggc gttacgctgg
    17641 acaagagtga atcgcgcacc gactggctgg ccagaccgct gaccgaacgt cagtgtgaat
    17701 acgcagcggc ggatgtctgg tatctgttac cgatcaccgc caagcttatg gtagaaacgg
    17761 aggcctccgg ctggctacct gcggcgctgg atgaatgccg cctgatgcaa atgcgtcgtc
    17821 aggaagtcgt tgcgccggaa gatgcctggc gtgatatcac caatgcctgg cagttacgca
    17881 cacgccaact ggcctgtctg caactgttag ccgactggcg actgcgcaag gcgcgagagc
    17941 gtgatctggc ggtgaacttt gtcgtgcgtg aagagcattt gtggtcggtc gcgcgttata
    18001 tgccgggaag tttaggcgaa ctggacagcc tgggtttatc cggtagcgaa atccgctttc
    18061 acggtaaaac gctgctggcg ctggtggaaa aagcgcagac attgccggaa gatgccttac
    18121 cgcagccgat gcttaacctg atggacatgc ctggttatcg taaagcgttt aaagcgatta
    18181 agtcgctgat tactgacgtg agcgaaacgc ataagatcag cgccgaattg ctggcatcgc
    18241 gtcggcaaat caaccaactg ctgaactggc actggaaact gaaaccgcag aacaatttgc
    18301 cggagctgat ttccggctgg cgtggtgagc tgatggcgga agcattacac aatttattgc
    18361 aggaatatcc gcagtaaaat cttccgaagc cggactgggc gcgctcagcg ccacatccgg
    18421 ctttggcaaa ctacaaatcc aacaccaggc gtttaccctt cgcacgcgaa caacagatca
    18481 acatactttg ctggctggca cgctcttcat cgctaaaata ctgatcccga tggtcagctt
    18541 caccttccag tattgccgtt tcgcaggttc cgcatacccc ttcacgacat aaacattcca
    18601 ctttcgcggc tttattattt tcaataacct gcaaaatagt catctcttcc ggcaccacaa
    18661 actcttttcc ggaacgggca agcaccaggg taaatgcatc gccggttttg tcttcgatag
    18721 caaattgctc aaagtgcagt gtatcggcgg cgatgtccag acgcgcagct tcacttctta
    18781 ccgcttcaat tagcgcctcg gggccacagg tataaacgtg tgtgccaggt tcgatatccg
    18841 ccaataatcg cgctaattcc agtcgtgttc cggttgatga atgatgcaaa tggactttct
    18901 cagcctgcgg atgctggact agctcagcac gaaatgcgca actttctgga tttcgcgagc
    18961 agtaatgtag ctgccagtcg atgtcgctgt gttgcaactc tgccatgtgc gacaggaaag
    19021 gggggatacc aataccgccc gcgataaaca gatgctttct ggctgaggga attagcgcaa
    19081 acaggttatt aggcgttgag atcgttaacc gatcgcccac ttttacctgc tgatgcaaaa
    19141 agcgggaacc gccgcgcgag ttttcttcca gtcgaacggc aatctgataa ctagaggtgt
    19201 tatgcggtga actcagtagt gaatacgcat tgctgtactg gttatcacca tcgctcatct
    19261 gcactaggat gtgacttcct ccggtgaacg caggcagcgg tttgccatcg gttgccacca
    19321 gcgtgaagcg tttcacctgt tcggtaaggg gttcaacctg gctcacctgt acttcaaaca
    19381 tttgatagtc tgacattgct gtactccatc aaaaatcagc ccagcggcgg aaaataccgc
    19441 cgccgatgtc attagtcctt aaacacctgc gccagcagat tatggaaatg ggcgatacca
    19501 tgttcggaaa tgccactacc gctactgtcg gccatgatgc gcccctgacc acgatagcca
    19561 cgcgatttca gccctttctg tacgctttca accagacgta aatcttccgg atggaacaca
    19621 tcgcgatacc actcaatcag cgatttttgc tcgtcggtta actcttcatt ggtgaagtaa
    19681 atatcgtagt tttgcagggt agtttcagaa tccaccggga attcataaat gaccgtcatc
    19741 atccctttga tcggggtgac gttcagcatc gtgcacggcc acagccagaa accgtggaat
    19801 gccgcatccg taccctcttc aaatttaaac gactgttcgg acggtttggc gaaaccgtat
    19861 tgcagcgtcc agttaccgtg catggtgtgc cagtaacgat caacctgtac ggagtcggag
    19921 aaacctagat gcgccggacc acagtgatag cactcgagat agttatcgac aatgttcttc
    19981 cagttggcag gcgtgcgggt ggtaaagcgg gccgctagtt tcagattgtg gacttccggg
    20041 caggcttcca gcactttcgc gcccagaccg gtgtattgat tcacttgaag tacgaaaaaa
    20101 aaccgggagg acattggatt attcgggttc tgatgggatt agatttggtg gggcttgcaa
    20161 gcctgtagcg caaattttag tcgttaatca atgaaacgcg aaagatagta aaaaattgct
    20221 tttgtttcat tgaaaatacg aaaaacaaaa acactgcaaa tcatttcaat aacagcttca
    20281 aaaaacgttc aaaaccgata acaaccaggc tgtaaccaaa tgactcatat cacaaatcag
    20341 ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg
    20401 ttcatggttg ttatacggct ttttttacat cttgtggttc ttgtgaagga gctacaagac
    20461 cttcctgttc ataaatacca tactttgaca aaatcttttt acgagtatct gttggtaatg
    20521 attcaagcat tttggctgtt tgtaaggttt gctgcgaaat accttgcaaa tcagcctgga
    20581 tggaatcatc ggaagaaaaa atctcaggca taagagcttc aaatcctttc tttctgaaca
    20641 actcaatcgc agaatgcagt tccttgtcct ctaacaattc gacaattgcg atcagtttag
    20701 ttttcaatgt gctgcggtca ggttgagaat ttttattctc ttgattattt ttatgctcaa
    20761 ccattggcat gctctgactt tggataaacc actctattgg acgccctgtt gcgtaggcta
    20821 tttgtgctaa tcgatctaaa gacgggtatg tcttgccaga caggtagttt ctgataaccc
    20881 catcagaaag tccgcactta gctgcaaacg cccggcttga ttctccattc attgcctctt
    20941 caaggcgttc cttgaaacgc attatttccg ggggcataaa aatgcgctct gcatttttgc
    21001 tttctttttc acctgtcatg cccaatctct ctgttatata aagaatttta cagaattctt
    21061 tgatttgcac tggttaaaat aaaaatgagc atagctcttg aattgagcat ttttctgcgg
    21121 tatcgttttt cccgatagcg gtagccggta tcagttaccg aaaacgttaa gagagtaagg
    21181 atcgcagaat gtcagcaaaa aaccaagact ggcacgcaga gcagataaag gctgctgttc
    21241 gtatgaaggg gaagacacta tctcaattat ccagaga
//
LOCUS       DAECNO010000066        21256 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.066, whole genome shotgun sequence.
ACCESSION   DAECNO010000066 DAECNO010000000
VERSION     DAECNO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.066"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..793
                     /locus_tag="KKO08_002593"
     CDS             <1..793
                     /locus_tag="KKO08_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBD6318792.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="KKO08_002594"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="KKO08_002594"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBD6318793.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="KKO08_002595"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="KKO08_002595"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBD6318794.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="KKO08_002596"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="KKO08_002596"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBD6318795.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="KKO08_002597"
     CDS             complement(4991..7225)
                     /locus_tag="KKO08_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBD6318796.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="KKO08_002598"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="KKO08_002598"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBD6318797.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="KKO08_002599"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="KKO08_002599"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBD6318798.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="KKO08_002600"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="KKO08_002600"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD6318799.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="KKO08_002601"
     CDS             complement(12980..14320)
                     /locus_tag="KKO08_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBD6318800.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="KKO08_002602"
     CDS             complement(14322..15674)
                     /gene="gudP"
                     /locus_tag="KKO08_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBD6318801.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="KKO08_002603"
     CDS             complement(16108..16557)
                     /locus_tag="KKO08_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBD6318802.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="KKO08_002604"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="KKO08_002604"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBD6318803.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="KKO08_002605"
     CDS             complement(17357..17686)
                     /locus_tag="KKO08_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBD6318804.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="KKO08_002606"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="KKO08_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBD6318805.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="KKO08_002607"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="KKO08_002607"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBD6318806.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="KKO08_002608"
                     /gene_synonym="ygdH"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="KKO08_002608"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBD6318807.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5226 a         5671 c         5073 g         5286 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc atgccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatat
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctccc
//
LOCUS       DAECNO010000067        21094 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.067, whole genome shotgun sequence.
ACCESSION   DAECNO010000067 DAECNO010000000
VERSION     DAECNO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.067"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..60)
                     /locus_tag="KKO08_002609"
     CDS             complement(<1..60)
                     /locus_tag="KKO08_002609"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318808.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            171..1661
                     /locus_tag="KKO08_002610"
     CDS             171..1661
                     /locus_tag="KKO08_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD6318809.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1713..2870
                     /locus_tag="KKO08_002611"
     CDS             1713..2870
                     /locus_tag="KKO08_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBD6318810.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2836..2958)
                     /locus_tag="KKO08_002612"
     CDS             complement(2836..2958)
                     /locus_tag="KKO08_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318811.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3161..4846
                     /locus_tag="KKO08_002613"
                     /pseudo
     CDS             3161..4846
                     /locus_tag="KKO08_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4884..7244
                     /locus_tag="KKO08_002614"
     CDS             4884..7244
                     /locus_tag="KKO08_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD6318812.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7301..10084
                     /locus_tag="KKO08_002615"
     CDS             7301..10084
                     /locus_tag="KKO08_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBD6318813.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10445..11845
                     /locus_tag="KKO08_002616"
     CDS             10445..11845
                     /locus_tag="KKO08_002616"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD6318814.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            12001..13536
                     /gene="gadC"
                     /locus_tag="KKO08_002617"
     CDS             12001..13536
                     /gene="gadC"
                     /locus_tag="KKO08_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBD6318815.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLA
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            13667..14986
                     /locus_tag="KKO08_002618"
     CDS             13667..14986
                     /locus_tag="KKO08_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBD6318816.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLHDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            15284..15325
                     /locus_tag="KKO08_002619"
     CDS             15284..15325
                     /locus_tag="KKO08_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318817.1"
                     /translation="MVQLVDLARCVSF"
     gene            15363..16508
                     /gene="dosC"
                     /locus_tag="KKO08_002620"
     CDS             15363..16508
                     /gene="dosC"
                     /locus_tag="KKO08_002620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBD6318818.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            16433..17050
                     /locus_tag="KKO08_002621"
                     /pseudo
     CDS             16433..17050
                     /locus_tag="KKO08_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            17308..17640
                     /gene="ddpX"
                     /locus_tag="KKO08_002622"
                     /pseudo
     CDS             17308..17640
                     /gene="ddpX"
                     /locus_tag="KKO08_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            17654..18550
                     /locus_tag="KKO08_002623"
     CDS             17654..18550
                     /locus_tag="KKO08_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBD6318819.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            18547..19533
                     /locus_tag="KKO08_002624"
     CDS             18547..19533
                     /locus_tag="KKO08_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBD6318820.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            19526..20452
                     /locus_tag="KKO08_002625"
                     /pseudo
     CDS             19526..20452
                     /locus_tag="KKO08_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(20508..20939)
                     /gene="osmC"
                     /locus_tag="KKO08_002626"
     CDS             complement(20508..20939)
                     /gene="osmC"
                     /locus_tag="KKO08_002626"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBD6318821.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         5681 a         4939 c         4954 g         5520 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttacccttta
      121 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
      181 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      241 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      301 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      361 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      421 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      481 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      541 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      601 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      661 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      721 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      781 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      841 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      901 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      961 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     1021 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     1081 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1141 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1201 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1261 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1321 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1381 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1441 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1501 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1561 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1621 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1681 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1741 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1801 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1861 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1921 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaatg
     1981 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     2041 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     2101 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2161 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2221 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2281 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2341 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2401 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2461 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2521 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2581 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2641 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2701 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2761 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2821 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2881 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2941 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     3001 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     3061 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3121 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3181 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3241 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3301 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3361 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3421 aatatccgta aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3481 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3541 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3601 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3661 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3721 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3781 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3841 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3901 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3961 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     4021 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     4081 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4141 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4201 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4261 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4321 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4381 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4441 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4501 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4561 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4621 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4681 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4741 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4801 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4861 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4921 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4981 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     5041 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     5101 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5161 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5221 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5281 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5341 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5401 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5461 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5521 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5581 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5641 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5701 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5761 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5821 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5881 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5941 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     6001 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     6061 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6121 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6181 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6241 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6301 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6361 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6421 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6481 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6541 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6601 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6661 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6721 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6781 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6841 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6901 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6961 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     7021 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     7081 gcttgtcctg gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7141 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7201 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7261 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7321 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7381 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7441 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattgcagga
     7501 agaagacaat gagctcggcg tggctcattt tgtagaacat atgatgttta acggcacaaa
     7561 aacatggccg ggtaataaag tcatcgaaac atttgagtca atgggcctgc gttttggtcg
     7621 cgatgttaat gcctatatca gctatgacga aacggtgtat caggtgagtt tgccgactac
     7681 gcagaaacaa aatctgcaac aagtgatggc aatcttcagt gaatggagta atgccgcaac
     7741 ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt actgaggaat ggcgtgccca
     7801 tcaggatgcg aaatggcgca cctctcaggc gcgccgccct ttcctgctgg ctaatacccg
     7861 taatttagac cgtgaaccta tcggcctgat ggatactgtc gccacggtca cgccggcaca
     7921 attgcgccaa ttttatcaac gctggtatca accaaataat atgaccttta tcgtggtcgg
     7981 cgatatcgac agtaaagaag cgctggcgct gataaaggat aatttaagta agcttccggc
     8041 taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa
     8101 tatcattaat gataaagaaa accgggtgaa cggcatcgca ctctattatc gcctgccaat
     8161 ggtacaagtg aacgatgagc aaagctttgt cgaacaagct gaatggagca tgttagttca
     8221 gctgttcaat caacgtctgc aggaacgcat acagtcgggc gagttgaaga ctatttctgg
     8281 cggcactgcg cgcagcgtta aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa
     8341 tgcacgagac gataatatgc aggatgctgc gaatgcatta atggcagagt tggcaaccat
     8401 tgatcagcat ggtttttctg ctgaagaact cgatgatgtc aaatctaccc gcctcacctg
     8461 gctgaaaaat gcggttgatc agcaagctga acgtgattta cgtatgctga ccagtcgcct
     8521 ggcatccagc tcattaaata atacgccgtt cttgtcgccg gaagagacat atcaactttc
     8581 gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg gaaaaatggc agcagttaag
     8641 aaagaaccag gacgcatttt gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc
     8701 attgtctcct gcggctatcc tggcgctgga aaaagagtac gccaacaaaa agctggcggc
     8761 ttacatcttc ccaggcagaa atttatcgtt aacagtagac gctgacccac aggcggaaat
     8821 tagcagcaaa gaaacgctgg ctgagaatct gacatcatta acactttcca atggtgccaa
     8881 ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa
     8941 taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt atcgcgctgg caaataaagc
     9001 agttagcgga agcggcgttg gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga
     9061 aaactcggta accatgagca gtaaagtcag tggcatgaat acgttgctct ccgttagcgc
     9121 gcggactaat aaccctgaac ctggtttcca gttgattaac cagcgaatca cccacagcac
     9181 gattaacgac aatatttggg catcgctaca aaatgctcaa attcaggcgt tgaaaacgct
     9241 cgaccagcgt ccagcggaga aattcgccca gctgatgtat gagacgcgct atgctgatga
     9301 ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt actgccgcag atgcgctggc
     9361 tgccgatcgc cagttgtttt catctccagc ggatatcacg tttgtcattg tcggtaatgt
     9421 cgcagaagac aaactcgtgg cgttaattac gcgttactta ggctcaatca aacactctga
     9481 ttcgccatta gccgcaggta aaccattaac tcgcgcgacg gacaacgcat cggttactgt
     9541 aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg aagcgttatg attcccggac
     9601 acctgttaat ctggcgacgc gtatggcgct cgatgctttt aacgtcgcac tggcaaaaga
     9661 tctacgcgtt aatattcgtg aacaggcatc tggggcatac agcgtttctt ctcgcctctc
     9721 ggttgatcct caggctaaag atatcagtca tttgctggct tttacttgtc aaccagaacg
     9781 acatgatgaa cagttaacgt tagcgaatga agtgatggtt aagcgtttga ctaaggggat
     9841 cagtgagcaa gaactgaacg aataccagca aaacgttcag cgcagcctcg atatccaaca
     9901 gcgtagcgtt caacaattag cgaacactat tataaatagt cttattcaat atgacgatcc
     9961 tgcagcatgg actgagcagg agcaattgtt gaaacaaatg acggtagaga atgttaacac
    10021 tgccgttaaa caatatcttt ctcatccggt aaatacttat accggagtat tattgccaaa
    10081 ataataacag ccccgtcaac acatcgttgg cggggatttt agcaatattc gctattttta
    10141 tgtaataatt ttataaatgc gttcaaaata ataaccaagt actaatagtg atattttaat
    10201 gcctgatttt tacgtgataa ttcaggaggc acagaaagcg cataaaaata acaacataaa
    10261 acacctttcc accacccaag aattctctat tacattgttt ttaatgaaaa aatatcattc
    10321 caacaatatc tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca
    10381 cttgcttact ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt
    10441 taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg
    10501 tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga
    10561 cgatgtcgca tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa
    10621 cctggccact ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc
    10681 cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg
    10741 ttgcgtaaac atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt
    10801 tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg cgatgaaatg
    10861 gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa acctggtgtg
    10921 cggtccggtg caaatctgct ggcataaatt cgcccgctac tgggatgtgg agctgcgtga
    10981 gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg aagcctgcga
    11041 cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta actatgagtt
    11101 cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta tcgacatcga
    11161 catgcacatc gacgccgcca gcggtggctt tctggcaccg ttcgtcgccc cggatatcgt
    11221 ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccata aattcggtct
    11281 ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc cgcaggaact
    11341 ggtgttcaac gttgactacc tcggtggtca gattggtact tttgccatca acttctcccg
    11401 cccggcgggt caggtgattg cacagtacta tgaattcctg cgccttggtc gtgaaggcta
    11461 taccaaagta cagaatgctt cctaccaggt cgctgcctat ctggcggatg agatcgccaa
    11521 actggggccg tatgagttca tctgtaccgg tcgcccggac gaaggcatcc cggcggtttg
    11581 cttcaaactg aaagatggtg aagatccggg atacaccctg tacgacctct ctgaacgtct
    11641 gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca ccgacatcgt
    11701 ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga
    11761 agactacaaa gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca
    11821 gcagaacagc tttaaacata cctgataacg tttaacggta acggtgtccc gaaacgaacc
    11881 cgtttcggga taatttccaa agtctgttca ctggcattag caacggaaaa tattgttctg
    11941 aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa taccgtttta gggggataat
    12001 atggctacat cagtacagac aggtaaagct aagcagctca cattactcgg attctttgcc
    12061 ataacggcat cgatggtaat ggctgtttat gaatacccta ccttcgcaac atcgggcttt
    12121 tcattagtct tcttcctgct attaggcggg attttatggt ttattcccgt gggactttgt
    12181 gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc ctgggtatca
    12241 aatactctgg gtccgagatg gggatttgca gcgatctcat ttggctatct gcaaatcgcc
    12301 attggtttta ttccgatgct ctatttcgtg ttaggggcac tctcctacat cctgaaatgg
    12361 ccagcgctga atgaagaccc cattaccaaa actattgcag cactcatcat tctttgggcg
    12421 ctggcattaa cgcagtttgg tggcacgaaa tacacggcgc gaattgctaa agttggcttc
    12481 ttcgccggta tcctgttacc tgcatttatt ttgatcgcat tagcggctat ttacctgcac
    12541 tccggtgccc ccgttgctat cgaaatggat tcgaagacct tcttccctga cttctctaaa
    12601 gtgggcacac tggttgtatt tgttgccttc attttgagtt atatgggcgt agaagcttcc
    12661 gcaactcacg tcaatgaaat gagcaacccc gggcgcgact atccactggc tatgttactg
    12721 ctgatggtgg cggcaatctg cttaagctct gttggcggtt tgtctattgc gatggtcatt
    12781 ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa cctttaccgt tctgatgtcc
    12841 catgtggcac cggaaattga gtggacggtt cgcgtgatct ccgcactgct gttgctgggt
    12901 gttctggcgg aaatcgcctc ctggattgtt ggtccttctc gcgggatgta tgtcacagcg
    12961 cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt accggtaacg
    13021 ctggtcattt cgcagctggt gattacttct atcgcgttga tcatcctcac caataccggt
    13081 ggcggtaaca acatgtcctt cctgatcgca ctggcgctga cggtggtgat ttatctgtgt
    13141 gcttatttca tgctgtttat tggctacatt gtgttggttc ttaaacatcc tgacttaaaa
    13201 cgcacattta atatccctgg tggtaaaggg gtgaaactgg tcgtggcaat tgtcggtctg
    13261 ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc cggataacat ccagggtgat
    13321 tctaccgata tgtatgttga attactggct gttagtttcc tggtggtact tgccctgccc
    13381 tttattctct atgctgttca tgatcgtaaa ggcaaagcga ataccggcgt cactctggag
    13441 ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc tgcacccgcg tgcacgttca
    13501 ccacactata ttgtgatgaa tgacaagaaa cactaaccat gtctgaatgg ttataagaca
    13561 agggagcgat aattcatcgc tccctttttc gtgcttgctg tcgtattgac ttaaccggga
    13621 taagtacgag aatgagcgca catctgttta ccggaaacca gcacatatgg atatctgctc
    13681 ccgaaacaag aaattaacga ttagaagacc agcgatactg gttgcattgg cacttttact
    13741 gtgtagttgt aaaagcacgc ctccagagtc catggtgaca ccaccagcag gttcaaagcc
    13801 accagccacg acgcaacaat cgtcacaacc gatgcgtggc atctggctgg ccacggtttc
    13861 tcgcctcgac tggccaccgg tttcctcggt taacattagt aatcccacca gccgagcccg
    13921 tgtacaacaa caggcgatga tcgacaaact ggatcatctc caacgtctcg gcataaacac
    13981 ggtctttttc caggtcaagc cggacggtac cgccctgtgg ccatcgaaaa ttttgccgtg
    14041 gtccgatctt atgaccggta agattggtga aaatccgggt tacgatccgc tgcaattcat
    14101 gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc tggtttaacc cctatcgcgt
    14161 atcggttaat acgaagcccg gtactatcag ggaactgaat agcactctgt ctcaacaacc
    14221 ggcgagcgtc tatgtgcaac accgtgactg gatcagaacg tctggcgatc gctttgtcct
    14281 cgacccgggc atccctgagg ttcaggactg gatcacatca atagtcgctg aagtggtttc
    14341 ccgctatccg gtagatggcg tgcagtttga cgactatttc tatactgaat caccgggttc
    14401 acggctacat gataacgaaa cgtaccgtaa atacggaggc gcatttgcgt caaaagcaga
    14461 ctggcggcgc aacaatactc agcagttaat tgcaaaggta tcacacacca ttaaaagcat
    14521 taagccggga gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc gatcacacga
    14581 tccgctcggt tccgataccc gaggcgcggc agcctatgac gaatcctacg cagacacccg
    14641 tcgatgggtg gaacaaggat tgctggatta cattgctccc caaatttact ggccgttctc
    14701 acggagtgcc gcgcgttatg acgtgttggc aaaatggtgg gcggatgtcg ttaaaccaac
    14761 caggacccgc ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt caaagataga
    14821 gccagactgg atgattaacg gcggcgtacc ggaactgaaa aagcagctcg atcttaacga
    14881 tgcggtacca gaaattagcg gcaccatctt gttccgtgag gactatctga ataaaccgca
    14941 gactcaacaa gcggtcagct atctgcaaag tcgttggggc agttaaacta ccatgcttac
    15001 tttgtaacaa gccggtgctt tacccaccgg cttgttacac cttgtgaaat atcccactag
    15061 taatcatgct tacataagtc aaattaacca cgcaattaac cacacttagt taaacagcta
    15121 taaatcccta ataaaaatgt atgaatatga aaaaccatac gatattcgct gtcaccgaaa
    15181 aataattacg atgggctata ctcttctgaa gaacagtagc gtagattgaa actgtcttaa
    15241 gtcgcttatt tcttttcagt atatcttcat atttcaggag aatatggtgc agttagtcga
    15301 tctcgcacgt tgcgtttcgt tttgattccg tctgatttcc cagccttata agggtgtgaa
    15361 ttatggagat gtattttaaa agaatgaaag atgagtggac cggacttgtc gaacaggcag
    15421 atcctctcat tcgtgctaaa gccgcagaaa ttgcccttgc gcatgctcat tatctgagta
    15481 tcgagtttta tcgaattgtc cgcatcgacc cgcatgccga agaattcttg agtaatgaac
    15541 aagttgagcg gcagttgaaa agtgcgatgg aacgctggat tattaacgtg ctttctgccc
    15601 aggttgacga tgtcgaaagg ctaatacaaa tccagcatac cgtcgcggaa gtgcatgccc
    15661 gcataggaat tccggtagaa attgtcgaga tggggtttcg ggtgctgaaa aaaatcctct
    15721 atccggtaat cttctcttcg gattattccg ccgcagaaaa acttcaggtc taccatttct
    15781 cgattaacag tattgatatc gcgatggaag tgatgacccg cgcgtttacc tttagtgaca
    15841 gtagtgcctc aaaggaagat gaaaactatc gtatcttctc gttactggaa aacgccgaag
    15901 aagaaaaaga acggcaaata gcctcaatac tttcatggga aatagatatt atctataaag
    15961 tcctgctgga ttctgattta ggcagcagtt tgcctttaag ccaggctgat tttggcctgt
    16021 ggtttaacca taaaggtcga cattatttta gcggcattgc cgaagtgggc catatctccc
    16081 gtctgattca ggatttcgac ggtattttca atcaaaccat gcgtaacacc aggattttga
    16141 ataacagaag tttgcgggta aaatttctat tacagataag aaataccgta tcgcagatta
    16201 ttaccttgct gcgtgaattg tttgaagaag tatcgcgcca cgaagtcggt atggatgtac
    16261 tgacgaaatt acttaaccgc cgtttcctgc cgactatctt caaacgcgaa attgcccatg
    16321 ccaaccggac cggtacaccg ctgtcagtgc tgattattga cgttgataaa ttcaaagaga
    16381 tcaacgatac gtggggccat aacactggcg atgaaattct gcgtaaagtc tcattcctct
    16441 cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg
    16501 tcagttagca gaatggcgta gccagaatat tcatatcccg gcgttatccg tgaacttgtc
    16561 ggcgctgcac tttcgcagta atcagctgcc taatcaggtg tctgatgcaa tgcaagcctg
    16621 gggtattgac ggccaccagc tgacggtgga aatcacggaa agcatgatga tggaacacga
    16681 taccgaaatc tttaagcgca ttcagatcct gcgtgatatg ggcgtgggtt tatcggtaga
    16741 tgattttggc acgggctttt ccggattatc ccgcttagtc agtcttccgg taacggaaat
    16801 caaaattgac aaaagctttg tcgatcgttg tctgacggaa aaacgcatcc tggccttact
    16861 tgaagccatt accagcattg ggcaaagcct caatttaacc gtcgtggcgg aaggggtcga
    16921 aaccaaagag caatttgaga tgctacgtaa gatccactgt cgcgttattc agggatattt
    16981 cttttcccgc ccccttcccg ccgaagaaat tccaggctgg atgagcagcg tgttaccgct
    17041 gaaaatctga caaattcctc tcgcccgcac tcgcgggttt tcctttaagg tgacactgtc
    17101 acttaaatac tgtgatttca gctacgattc cgggagattc cttccttaac tttccctctt
    17161 gcaccaaaac tgcactacgc tgcacaaatg ttgatcaaaa gttaaaaaat ataactttaa
    17221 gccagcattc tgccgcttat cccggcatgg catgagatct gcataagcgg aaagcgcagc
    17281 aatttttgtc ttatacagga accgtttatg tcggatacca ccgaactggt tgatttagcc
    17341 gtgatcttcc ctgatctgga gatcgaattg aaatatgcct gcgttgataa catcacaggt
    17401 aaagctattt atcagcaagc gcgttgtctg ctacataaag atgcgattac cgcgctggcg
    17461 aaaagtatca gtatcgccca gctgtcaggc ttacaactgg tgatttacga tgcgtatcgc
    17521 ccacaacaag cacaggcgat gttgtggcaa gcctgcccag acccgcaata tgttgttgat
    17581 gtgacggtcg gttctaatca cagccgtggc acggcgctcg acctgacgct tcgtgatgac
    17641 gtggaggtgg tgaatgatgc taagcgagga aacgtccgcc gtacgcccac aaaaacaaac
    17701 gcgatttaac ggtgcaaaac tggtatggat gctgaaaggc agtccgctca ccgtgaccgg
    17761 cgcagtcatc attgtattaa tgcgactgat gatgattttt tcaccgtggc tggcgacgca
    17821 tgatcccaac gccattgatt taaccgcccg ccttttgccg ccttctgcgg cgcactggtt
    17881 tggcaccgat gaagtgggac gcgatctgtt tagccgcgta ctggtcggca gtcagcaatc
    17941 aattctcgcc ggattagtgg tggtcgccat tgcgggtatg attggttcgc tactcggatg
    18001 tctatccggt gtgcttggcg gacgcgcaga cgccatcatc atgcgcatca tggacattat
    18061 gctgtcgatt ccttcgttgg tactgacaat ggcactggct gccgctctcg ggccgagttt
    18121 atttaacgcc atgctggcga ttgctattgt gcgaattccc ttttatgtgc gcttggcgcg
    18181 ggggcaagca ttagttgtac gccagtatac ctatgttcag gcggcgaaaa cctttggcgc
    18241 gtctcgttgg catctgatca actggcatat tttacgtaac tccctaccgc cgctgatcgt
    18301 gcaggcatcg ctggatatcg gtagcgcgat tttaatggcc gccacgttgg gatttattgg
    18361 cctaggtgct caacaaccga gtgctgaatg gggcgcgatg gtagcgaatg gtcgcaacta
    18421 tgtactcgat cagtggtggt attgcgcatt tccgggggca gcgattttgc ttacctccgt
    18481 cgggtttaat ctctttggtg atggtattcg cgatctgctt gacccgaaag caggaggaaa
    18541 gcagtcatga cccaacccgt tctggacatt caacagctgc atttgagttt ccccggtttt
    18601 aacggtgatg ttcacgcgct caacaatgtg tccttgcaga ttaaccgcgg cgaaattgtc
    18661 ggtctggtgg gagaatccgg ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg
    18721 ctaccgacgg gcagttattg cgtacatcgg gggcagattt cactgctggg agaagatgtt
    18781 cttaacgctc gggaaaaaca gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt
    18841 caggaaccga tgaccgccct taatccgaca cgtcgaatag gtcttcagat gatggacgta
    18901 atccgccatc atcaaccaat aagtcgtcgg gaagccagag ctaaagcgat tgccctgctg
    18961 gaagagatgc aaatcccgga tgccgtggaa gttatgtcgc gctatccgtt tgagctttca
    19021 ggtggtatgc gccagcgggt aatgattgcg ctggcattct cctgcgagcc gcaattgatt
    19081 attgccggcg aaccgacgac ggcgctggac gtcacagtac agttgcaggt actgcgtttg
    19141 cttaaacata aagcacgcgc cagtggaact gcggtactgt tcatcagcca tgatatggcc
    19201 gtggtgtcgc aactgtgcga tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc
    19261 ggcgggacgg cagacgttat ccatcatccc cggcatccgt ataccattgg tttgctgcaa
    19321 tgcgcaccag aacatggagt accacgccag ccattacccg ccattccagg gacggtacca
    19381 aacctcaccc atttgcctga cggctgcgct tttcgcgatc gttgctatgc ggcaggtgca
    19441 cagtgtgaaa acgtcccggc gctgacagcg tgtggtgaca acaaccagca ctgcgcctgt
    19501 tggtatccgc agcaggaggt cataagtgtc tgacacgtta ttaacgttac gcgacgtcca
    19561 tatcaatttt ccggcccgta aaaactggct tggtaaatct acggaacatg ttcatgccat
    19621 taatggtatt gatttacaga tccgccgtgg tgaaacctta gggatcgtcg gcgagtcagg
    19681 ctgcggcaaa agcaccctcg cacagctttt aatgggcatg ctgcaaccga gccacgggca
    19741 gtacatccgt tcaggctcac aacgcattat gcagatggtg tttcaggacc cgctctcttc
    19801 gcttaatccg cgcttaccgg tgtggcgcat catcacagaa tcgctctgga tagctaagcg
    19861 tagtagtgaa caacagcggc gagcgttggc agaggagttg gctgtgcagg tgggtattcg
    19921 tccggagtat ctcgaccgcc tgcctcatgc gttctccggc gggcagcggc aacgcatcgc
    19981 cattgccaga gcactctctt cgcagcctga cgtgattgtg cttgatgagc caacctcggc
    20041 gctggatatc tccgtgcagg cgcagatcct caatttgctg gtaacgctac aggaaaatca
    20101 cgggctgact tatgtgctga tttcacacaa tgtctcggtg atacgtcata tgagcgatcg
    20161 ggtggcggtg atgtatctcg ggcagattgt agaactgggc gacgcgcaac aggtgttgac
    20221 tgcttcggca catccataca cccgattatt gctggattcc ctccccgcca ttgataaacc
    20281 gctggaggaa gaatgagcat tacgtaaaac ggatctgccg ggaaaccgca cgttgccgca
    20341 aggctgtttt ttctacgaac gttgccctct ggcaacccac ggatgtgaag tccggcaatc
    20401 attagcaata agggaggacg gacgtgagct ccggtgctgg cgggcgctgt agagatgcta
    20461 acaacgcatc aggcattcac gcgcctgacg cgtcatccgg caatgcttta cgatttcaac
    20521 tggtaatcca gcgtaatttc cgctttcagt acctgagaga ccgggcatcc tgcttttgct
    20581 ttctggatga tgccgtcaaa ggtagaggca tcaatacccg gcaccgcaac ttcactcttc
    20641 agtgcgattt tcgtaatcgc aaaaccggca tccactttat ccagcgacac atcggcggtg
    20701 gtatcaattg atgttggcgt gaatcccgct tcccccagca ttaatgaaag cgccattgag
    20761 aaacatgcgg catgcgctgc gccaatcagt tcttcagggt tggttccttt ttcgccttca
    20821 aaacgcgtgt taaatccata cggctgttgg ttcagcacgc cgctctcggt ggatactgtt
    20881 cccttcccgc gtttgatatc gccttcccag tgtgcctgac ctttcttatg gattgtcatt
    20941 gttgctctcc tgtgggctta aaaaggaaat tatagactac gtgagaaaca gcacgaggat
    21001 aagcaggata ttccgaataa aatacggcga aaatattact tctgcactta aattaaattc
    21061 ccacaataga taaccgggaa tatatatctc aaga
//
LOCUS       DAECNO010000068        20840 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.068, whole genome shotgun sequence.
ACCESSION   DAECNO010000068 DAECNO010000000
VERSION     DAECNO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.068"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(23..1021)
                     /gene="fbp"
                     /locus_tag="KKO08_002627"
     CDS             complement(23..1021)
                     /gene="fbp"
                     /locus_tag="KKO08_002627"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBD6318822.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1197..2570
                     /gene="mpl"
                     /locus_tag="KKO08_002628"
     CDS             1197..2570
                     /gene="mpl"
                     /locus_tag="KKO08_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBD6318823.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2653..3204)
                     /locus_tag="KKO08_002629"
     CDS             complement(2653..3204)
                     /locus_tag="KKO08_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBD6318824.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3298..4650
                     /gene="pmbA"
                     /locus_tag="KKO08_002630"
     CDS             3298..4650
                     /gene="pmbA"
                     /locus_tag="KKO08_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBD6318825.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4833..5219
                     /gene="cybC"
                     /locus_tag="KKO08_002631"
     CDS             4833..5219
                     /gene="cybC"
                     /locus_tag="KKO08_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBD6318826.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5264..5728)
                     /gene="nrdG"
                     /locus_tag="KKO08_002632"
     CDS             complement(5264..5728)
                     /gene="nrdG"
                     /locus_tag="KKO08_002632"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBD6318827.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5886..8024)
                     /gene="nrdD"
                     /locus_tag="KKO08_002633"
     CDS             complement(5886..8024)
                     /gene="nrdD"
                     /locus_tag="KKO08_002633"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBD6318828.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8418..10073)
                     /gene="treC"
                     /locus_tag="KKO08_002634"
     CDS             complement(8418..10073)
                     /gene="treC"
                     /locus_tag="KKO08_002634"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBD6318829.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10123..11544)
                     /gene="treB"
                     /locus_tag="KKO08_002635"
     CDS             complement(10123..11544)
                     /gene="treB"
                     /locus_tag="KKO08_002635"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBD6318830.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11663..12610)
                     /gene="treR"
                     /locus_tag="KKO08_002636"
     CDS             complement(11663..12610)
                     /gene="treR"
                     /locus_tag="KKO08_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBD6318831.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12795..12848
                     /gene="mgtL"
                     /locus_tag="KKO08_002637"
     CDS             12795..12848
                     /gene="mgtL"
                     /locus_tag="KKO08_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBD6318832.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12989..15685
                     /gene="mgtA"
                     /locus_tag="KKO08_002638"
     CDS             12989..15685
                     /gene="mgtA"
                     /locus_tag="KKO08_002638"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBD6318833.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15891..16277)
                     /gene="ridA"
                     /locus_tag="KKO08_002639"
     CDS             complement(15891..16277)
                     /gene="ridA"
                     /locus_tag="KKO08_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBD6318834.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16350..16811)
                     /gene="pyrI"
                     /locus_tag="KKO08_002640"
     CDS             complement(16350..16811)
                     /gene="pyrI"
                     /locus_tag="KKO08_002640"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBD6318835.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16824..17759)
                     /gene="pyrB"
                     /locus_tag="KKO08_002641"
     CDS             complement(16824..17759)
                     /gene="pyrB"
                     /locus_tag="KKO08_002641"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBD6318836.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17763..17897)
                     /gene="pyrL"
                     /locus_tag="KKO08_002642"
     CDS             complement(17763..17897)
                     /gene="pyrL"
                     /locus_tag="KKO08_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBD6318837.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18178..18573)
                     /locus_tag="KKO08_002643"
     CDS             complement(18178..18573)
                     /locus_tag="KKO08_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBD6318838.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18705..19418)
                     /gene="bdcA"
                     /locus_tag="KKO08_002644"
     CDS             complement(18705..19418)
                     /gene="bdcA"
                     /locus_tag="KKO08_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6318839.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19489..20082
                     /locus_tag="KKO08_002645"
     CDS             19489..20082
                     /locus_tag="KKO08_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBD6318840.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIAKRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20227..20679
                     /gene="tabA"
                     /locus_tag="KKO08_002646"
     CDS             20227..20679
                     /gene="tabA"
                     /locus_tag="KKO08_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBD6318841.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4963 a         5228 c         5520 g         5129 t
ORIGIN      
        1 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
       61 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
      121 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
      181 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
      241 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
      301 cgatataacg cgaggtatac gggcgattgg tggatttatc ttcttcctgg cagaatttaa
      361 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
      421 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
      481 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
      541 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
      601 ccggcgtgcc aacaggcgta acgcggcggt agatggagaa aatggtaccg acagagacgt
      661 taacatcgat gttggacgag ccatccaggg ggtccatcag caccacgtat tttgcgtgtt
      721 cacagccttc aaagacgaca atctcatctt cttcttcaga ggcaatcccc gcaacgatat
      781 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
      841 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
      901 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
      961 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     1021 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     1081 tcaatgttgc gctcaggtga atcgcgccag caaattgcgg attatcctga aatgcgtttc
     1141 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     1201 gcattcatat tttaggaatt tgtggcacat ttatgggcgg tctggcgatg ctggcgcgcc
     1261 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     1321 ttgagaagca aggcattgag ctgattcagg gttacgatgc cagccagctc gagccgcagc
     1381 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     1441 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     1501 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     1561 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     1621 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     1681 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     1741 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     1801 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     1861 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     1921 gtgagcaggg tcactggcag gcgaaaaagc tgaccaccga tgcttccgaa tgggaagttt
     1981 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     2041 acaatggcct gatggcgatt gcagcggctc gccatgttgg tgtagcgccg gcagatgccg
     2101 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     2161 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     2221 cggcgctgcg tggcaaagtt ggtggtacgg cgcgcattat tgctgtgctg gaaccgcgct
     2281 cgaataccat gaaaatgggg atctgcaaag acgatctggc accttcatta ggtcgtgccg
     2341 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     2401 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     2461 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     2521 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcttca
     2581 gcctgatata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     2641 caacaacgaa aattaccctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     2701 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagtgtacg
     2761 cagttgctga cgatcggcat cgggccacag attcaatact tcagcgatgg cgtcatcacc
     2821 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     2881 gtgacggttt ttcagcttat ccagcgcctg acgaataggc tctacgtcgc gctggcgcag
     2941 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     3001 ctcaatagcc gggcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     3061 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     3121 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     3181 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     3241 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     3301 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     3361 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     3421 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     3481 agtgatggcg cgctggggat cactgtttat caccagaacc gtaaaggtag cgcatcgtct
     3541 accgatttaa gcccgcaggc cattgcccgc accgtacagg cggcgctgga tattgcccgt
     3601 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     3661 ccgaatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     3721 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     3781 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     3841 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     3901 gagcgtgatt acgcctacac cattggccgc gcgatgagcg atctgcaaac gccagagtgg
     3961 gtcggggccg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     4021 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     4081 ggggcgatag cgggtggctc ggtttaccgt aaatctacct ttctgctgga ttcgctgggt
     4141 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     4201 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     4261 ggcatcctga ctcagtggct gctgaccagc tactcggcgc ggaaactggg gctgaaaagc
     4321 accggacatg cgggcggtat tcacaactgg cggattgccg gacaagggct aagcttcgaa
     4381 caaatgctca aagagatggg caccgggctg gtggtgacgg agctgatggg ccagggcgtg
     4441 agtgccatta ccggtgatta ttcccgtggt gcagcgggct tctgggtaga gaatggcgaa
     4501 attcagtatc ccgttagtga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
     4561 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
     4621 ttgccggaga tgaaaatcgc cgggcagtaa aaaactggcg cgacctgccg cgctataaac
     4681 taaaaaatta cacaaaatca ttcgcactgc atcgagacgg aaagtgaatg agggccgggg
     4741 agcgtactcg cagtacgtga cccgggcgaa tgagcgtagc cgtcgatgag gcagtgtgaa
     4801 ggatgaagtg taaataaaaa aggaagtgag caatgcgtaa aagcctgtta gctattcttg
     4861 cagtctcctc gttggtattc agttctgcgt cgtttgccgc tgatcttgaa gacaatatgg
     4921 aaaccctcaa cgacaattta aaagtgatcg aaaaagcgga taacgcggcg caagtcaaag
     4981 acgcgttaac gaagatgcgc gccgcagcgc tggatgcgca aaaagcaacg ccgccgaagc
     5041 tcgaagataa atcaccggac agcccggaaa tgaaagattt ccgccacggt ttcgacattc
     5101 tggtcggtca gattgacgac gcgctgaagc tggcaaatga aggtaaagta aaagaagcgc
     5161 aggctgctgc agagcaactg aaaacgaccc gcaacgccta tcaccagaag tatcgttaat
     5221 tcctcatttc cctgttgcct gcactcaggt aacagggaac ttatcatcgc aaatgatgca
     5281 ccacctgatt gctgctgccg cgccagatca gggaagggtc ttttaaatcc tgcacaaatt
     5341 tgccgtcgac cagcacgtta atcaaatcaa caacctgcat ttgcgcagcg ttgagttcgt
     5401 cgagtttata gcctgtccac acccagatgt ctttacccgg acactcggcg cggatgcgtt
     5461 ttaccagttt cagaatatcc ggcacgtttt gcggatgcag cggatcgccg ccggagaggg
     5521 aaatcccctg gcgtttgata cgagtgtcat tcagatcttt aatgatcttg tcttccattg
     5581 ctttggtaaa tggctgacca gaatttaccc gccatgtgct tttgttatag caaccggggc
     5641 attcatgaac acacccggag acaaacaggg tgcagcgagt gccggggccg ttgacgatgt
     5701 cgacaggata gtactgatga taattcatta tttcgcctgg cttatcagtc cgcgtgtagg
     5761 ctgcatccgg taatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc ctacagccgt
     5821 tgccaaatgt aggccggata aggcgtttac gccgcatccg gcatttgctt agcgcagagt
     5881 gaagattaac ctatctgccc attccccaaa tgtttaacgc ggcgcttaac ttcttcctgc
     5941 ttaccagcgt taaacggacg tgcatccggg ctacctaaat atccgcacac gcggcgagtt
     6001 accgacacac gggaggcgtc atggttacca catttcgggc aagtgaagcc tttgctggtg
     6061 cactcgaact caccggtaaa gccacactcg tagcactcat caatcggtgt attggtgccg
     6121 taatacggta catgctgata gctgtaatcc cagacatctt ccagcgcttt caggttgtgc
     6181 tgaatgtttg gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg cgggtaaggc
     6241 gcttcaaagt cgatcttgtc gtacgggttc accttcttct ccacatcaag gtggaaactg
     6301 ttggtgtagt aacctttatc ggtcacgccc ggcaccacgc caaactcagc agtatcgaga
     6361 cggcagaagc ggtcgcacag gttttcactc ggcgtgctgt aaagactgaa accataaccc
     6421 gtttcttctt tccactgatc cactgcctga cgcagacgtt caacaatggc gatacctttc
     6481 gcgcgaagct tctcgttgtc gtaaacatgc tcgccgctga acagcgcgtt aatggtttcg
     6541 tggatgccga tgtaacccag cgaaatagac gcacgaccgt ttttgaagat ttcagaaaca
     6601 tcatcgtcag cattcagacg tacgccacaa gcaccttcca tatagaggat cggggcgacg
     6661 cgcgctttca cgccttcgag acgagcgata cgggtcatca gtgccttacg tgccagcacc
     6721 agacgttcat ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc cagagcaata
     6781 cgcggcaggt tcaggctgat cacgccgagg ttgttacgac catcgtggat ctgctcgccg
     6841 ttttcatttt cccacacgcc gaggaagctg cggcagccca tcggggtttt aaacgaaccg
     6901 gtgacgttca ctacctgatc gtagttcagg atatccggat acatgcgctt gcttgcgcac
     6961 tccagcgcca gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt caggccatcg
     7021 cgaatcgcaa acaccagttt cgggaacacc gcagttttac ggtttttacc gaggcctgcg
     7081 atacggttac gcaggatcga ttcctgaatc aggcgcgatt cccagctggt gcccagacca
     7141 aaaccaaagg ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt tacttcgtac
     7201 tccagcgact ggaaggcatc gtagcactct ttgatggtac gagagttagc gtagccttcg
     7261 gcgtccggga tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct ggcggtaaca
     7321 aacggcgcca gcacttcatc gatacggtta atggtggtgc cgccataaat atggctggcg
     7381 acctgagcaa taatctgcgc agttaccgcg gttgccgtag agatcgactt cggcggttca
     7441 atctcggcgt tccccatttt aaaaccctgg gtcagcatgc ctttcaggtc gatcaacatg
     7501 cagttgaaca tcgggaagaa cggcgagtaa tcgagatcgt gatagtgaat atcgccacgc
     7561 tcatgtgcct gcaccacgtc acgcggcagc aggtgctggc gtgcatagtg tttagccacg
     7621 atcccggcca gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt ggcgttttcg
     7681 ttgagtaacg aggcgttggt ctgctcgacc agaccacgga tctcctggtt caggcgaccg
     7741 cgtttttcac gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg agccagttgt
     7801 ttgtatggac ccgacatcag ctggttttca actgcggtct ggatctcgtt gatatccacc
     7861 tggttgcgcc cctgcatctg ctcgttgaca accgcggcaa cagtggcgca ataatcggca
     7921 tcatcgactt ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg ctctgattta
     7981 aacggcactt tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc tccatatttt
     8041 taagaacagg ttatccacag aaattgggaa aggcgttttc cggttttttc gtttcgtttt
     8101 ccggtgcttt ccgcaatcgc catccgctta tccacaacgc cgcacctttt tcgtggggag
     8161 ctgtagtagc aattatagtc gattaataca acatattggg ttgggatgca ttttaaagtc
     8221 tatatatagt gctttgcatc aaggatgttt taactttttt tgatgtagct caaagtaaaa
     8281 agcagagcgt acggatgacg ggcgctacag cgttatgtaa attttttaat gaatttgctg
     8341 gttgaaaaat caacaaaaac aacatgctga cagacaaaac cccggaatga ccggcattat
     8401 cggggcttag ggaagattta cttctgtaac caccagacag cctcaaaagg ccgtaaattc
     8461 atggcacagg gttgtggtga ggcttcttcg taattatgca tcacaagctg ccagttgccg
     8521 cgcatttgcc ctggctgcca gggttggatc tcacggctaa ggttggcaat gaccagcaag
     8581 gtttgcccct tccattcacg gcgatagcac cacaatacag ggctgtttgg cagcagatcc
     8641 tggtaattgc cccatgtcag gatggcttct tgcttacgca gtgcgattaa cttttggtag
     8701 gtgtaaaaca cagaggagtc atcggccagc gcggcttcta cgttgatttg ttgatagtta
     8761 tcgcctaggc caatccacgg ttcgccagcc gtaaacccgg cattatcgcc gttgctccat
     8821 tgcatgggcg tgcggctgtt gtcacgggat ttactggcga ggattgccaa taactcgtcg
     8881 gcatcacgac catcgttgcg cagctcggca aacatattga ggctctccac gtcgcgatag
     8941 tcagtaatgc gcgtgaaatg cgggttggtc atgccaatct cttcgccctg gtagatatac
     9001 ggcgttccct gcatgccatg cagcaccatc gccagcattt ttgccgcagg cacgcggtat
     9061 tcaccttcat caccaaagcg agaaacaatg cgcggctgat cgtggttaca ccagaacaag
     9121 gcattccatg ccacgttgtg cattccttgt tgccagtggc ggaacaatgt tttcaacgcc
     9181 acaaagtcag gtttagccag cgtccatttt tcaccaccgg gataatcgac cttcaggtga
     9241 tgaaaattaa aggtcatcga caattcactg cctgtcagtg ccgcgtatcg ctggcaatgc
     9301 tcaaggctgg tggaggacat ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg
     9361 cggttcatct cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa gcgacgcccg
     9421 tcgccatcca ggtcttcagg gaaacgcggg tctttggaga tcagattcac cacatccagg
     9481 cgcaacccgt cgaccccacg atcggcccag aattcacaga cttttttcag ctctgcgcgt
     9541 accgctggat tctcccagtt gagatccgcc tgttctggtg caaagagatg caaatagtac
     9601 tgttcgcttt ccgcatgcca gcgccacgca ctaccgccaa attttgaacg ccagttgttc
     9661 ggtggcgttt ctggttcgcc atcgcgccag atataaaact ggcggtaagg gctttctttg
     9721 ttcagcgcct cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac catatcgaga
     9781 atgatacgaa tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc gtccagcgtg
     9841 ccgtaggtgg gatcaatcgc cgtatagttc gctacatcgt aaccgttatc gacctgggga
     9901 gagacataaa agggggttag ccagatggca tcaacgccca gtttatgcag atagtccagg
     9961 cgttggataa cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg aaaactcttt
    10021 ggataaatct ggtagataac gccgttttgc caccagtggg gaagattagt cattacgtta
    10081 ttcctgcaaa tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa tgtccagcgt
    10141 gcccaggcgg tatttccgct gatagataaa cgaggtgagt acaatcggga tgatgatggc
    10201 gatagccatt gccagcgcaa atacctgcca gtagctcggt tgaatagaga gaattcccgg
    10261 caggccccct acgccgatac cattcgccat aacgccgttc aggccgcata gcaatcctgc
    10321 cagaccagaa ccaatcatcg cgcacagcat cgggaagcga tatttcaggt tgatgccgta
    10381 cattgcaggc tcagtgaccc caagccaggc ggagatagcg gcaggcacgg agatctcgcg
    10441 ttcattgtgc ttgcggctgg aaatgataat gcctatcacg gcggagccct gagcgatatt
    10501 cgacagcgca atcagcggcc acactggcgt gccacccatg ctttgaatca tctgcaaatc
    10561 aatagcaagg gtggtctggt gtacgccggt gatcaccagc ggcgcgtaca ggaagccaaa
    10621 caatgccgca ccaatcggag caaagctgcc ggtcatcagg tgacgtaccg caaaggcaac
    10681 gccatcgcca atcatgcgac caaacggacc aatcagcgca tgggcgagga acaccgcgag
    10741 gatcagcgaa cagacgggca ccaccaccag atagaggtaa tccggtacga tgcgtttaag
    10801 gcgagtttca ataacgccca gcgccagccc ggctaacagt gccgggatca cctgcgcctg
    10861 atagcccact ttggcgatgc tgaacatgcc aaagtcccac acttccggca gctgctgccc
    10921 gagcagataa gcgttcatca gctgggggga aaccagtgtc acgccaagca cgataccaag
    10981 gatcggcgtg ccgcccattt ttttcaccgc tgaccagcaa atcccgaccg gcaggtagaa
    11041 gaaaatcgct tcaccgatca accacagaaa atcgtagatc gtttgcaggg aagggtacat
    11101 ttgcgccagc gtctgaccgt tgctcatggg caaatcgccg atcacattgc gaaaaccgag
    11161 gatcaaaccg ccgctaatca acgcgggcag caacgggaag aagatctccg cgaaatgaga
    11221 gatcaactgc tcatgccatt tcatattttg ccgggcggct ttttttacct gctctttatc
    11281 aacctgcgcc tgtccggttg acgctattag tgcttgatag taatcaccca cgttggtgcc
    11341 aatcaccacc tgaaattgcc cggcattggt gaaacagcct ttcaccatgg ggagttgctc
    11401 aatttctttc ggtctggcat tggccggttg gttgaggaca aagcgcaggc gagtaataca
    11461 gtggctcacc gtcgcaatat tgccgcgccc gccgaccagt tcaatcaacc gatcgatatc
    11521 cgtttggttt attttgctca tcataaagcc ccatggcaga tgacattttt ggttggctgc
    11581 agaatatatt gcgcggaaaa atttaaaaac gggaacgttc ccgaaacgca gcgaagatca
    11641 caatttatcg ttcaggaaac gatcaggaca gggtggcggg gatgatgatt tgttgcggtt
    11701 cgctgcgccc ggttacctgt gcgatcaatt ggcaggccgc ctggcgtcca gcttcggcgt
    11761 agccaggatc tacggtgacg atctccggat gaaggaattt cattaacggc gtattaccta
    11821 cgctcgccag ttgcaaggtg tcgatgcgtt gctcttgcag gtatttactt gcgccaagag
    11881 ccagcgtgtc ggttgcgcac agtaacgcgg tagtttcagg cgtaatcact tttgctacgt
    11941 tttcatagcc ttgcttcata gcaagccctg gcagagcggc aacgggatgc agtttatgcg
    12001 ctttgcagaa cgccaggtag gcttcgtgac gtcgcttccc ggtcgtcacg tcactatgcg
    12061 gcacgccgag ataactgata ttgcgatgcc cctggtcata gagccgttgc atcagaatct
    12121 tgattgcccc ttcgtcgtca taacagaccg aagcaaagcc ttttgcgtca cgcgccagca
    12181 gaaccagcga tgactgccag tgggctaaca tttcttcggt tatgccggta aaaccgaaca
    12241 gcactacgcc gtcgatatta cgccgtttca gcacccccaa atgttcggca actaattgtg
    12301 gggaaaactg attttccatc atgattggat cgtaaccttg ttcatagaac gctggcagca
    12361 tggtttgaac ggcgagattt tctgacaacg aatccagacg ggtaacaatg atggcgacca
    12421 ctttatcgct ttgcccacgc atagcgcgcg cagagcggga aggggaaaat ccatgctgat
    12481 tcatcactgc ttcaacacgc tcgcgcgtgc gctgactcac gccgctttcg ttattcagca
    12541 cccgggaaac cgtagatttc cccacgccgc ttaagcgcgc gatgtctttg atggtcagcc
    12601 gattttgcat cctgttgtcc cataacgtgt tgtttaatta tttgagccta actttacccg
    12661 tgcattcagc aatgggtaaa gtctggttta tcgttggttt agttgccagc aggtattata
    12721 tcgccataaa tgctacgaat attattggat tctccttatt atttgcggcg cttttttcac
    12781 ttaccggagg ttatatggaa cctgatccca cgcctctccc tcgacggaga ttaaaacttt
    12841 tccggtaagc ccgtcttttc acggcgttac cggatgcgta aggccgtgac gttttaatgt
    12901 ccctgctcag ctttattacc ttcaggtaag gcttcgccac gcctgaagac atttctgtac
    12961 tgtttcagac agtgcggagg gactccttat gtttaaagaa atttttaccc ggttcattcg
    13021 ccatttacct tcccgtctgg ttcatcgcga tccattgcct ggcgcgcagc agacagtgaa
    13081 tacggcggtc ccgccgtcct taagtgcgca ttgcctgaaa atggcggtga tgcccgaaga
    13141 agaattgtgg aaaacgttcg acacccatcc ggaaggatta aatcaggcgg aagtggaatc
    13201 tgcccgcgaa caacatggtg ataataaatt acccgcccaa caaccgtcgc cgtggtgggt
    13261 acatttatgg gtctgctatc gcaacccctt taatattttg ctcaccattc ttggtgctat
    13321 ttcttacgcc acggaagatt tatttgccgc aggtgttatc gcgctaatgg tcgctatttc
    13381 tacgttgctg aactttattc aggaagctcg ttccaccaaa gcggcagatg ccctgaaagc
    13441 gatggtcagt aataccgcga cggtactgcg agtaattaac gacaaaggcg aaaatggctg
    13501 gctggagatc ccgatagacc agctggtgcc cggcgatatt ataaaactgg cggcggggga
    13561 tatgatcccg gcagatttac gtatcttgca ggcgcgggat ctgttcgtcg ctcaggcgtc
    13621 gttaaccggt gagtctctgc ccgtggaaaa agccgctact actcgccagc cggagcacag
    13681 caatccgctg gagtgcgaca ccctgtgttt tatgggcacc accgtggtga gcggcacggc
    13741 acaagcaatg gtgattgcta caggagccaa cacctggttt ggtcaactgg cggggcgtgt
    13801 tagtgagcag gaaagcgagc cgaatgcctt tcagcaaggg atcagccgcg ttagtatgct
    13861 gctgattcgc tttatgctgg tgatggctcc ggtggtgttg cttatcaacg gttacaccaa
    13921 aggcgactgg tgggaagcgg cactgtttgc gctttcggtc gcggtaggct taacgccgga
    13981 aatgttgccg atgattgtta cctcgacgct ggcgcgcggc gcagtaaagc tgtcgaaaca
    14041 gaaagtgatc gtcaaacatc tggatgccat tcagaacttt ggcgcaatgg atattctgtg
    14101 cactgataaa accggcaccc tgacgcagga caaaattgtg ctggagaatc ataccgatat
    14161 ctccggtaaa accagcgaac gcgtgctgca tagcgcgtgg ttgaacagtc attaccagac
    14221 cggacttaaa aatctgcttg ataccgcggt gctcgaaggt acggatgaag agtcagcgcg
    14281 ttcgctggcc agccgttggc aaaaaattga tgagattccg tttgatttcg agcgtcgtcg
    14341 gatgtcggtg gtagtggcag aaaataccga acaccatcag ctggtttgca aaggtgcatt
    14401 gcaggaaatc ctcaatgtgt gttcgcaggt gcgtcacaat ggcgagattg taccgctcga
    14461 tgacaccatg ctgcgtaaga ttaagcgggt tactgatacg ctgaatcgtc aggggctgcg
    14521 cgtggttgcg gtggcgacga aatacctgcc agcgcgtgaa ggagattacc agcgggcgga
    14581 tgaatccgac ctgatcctcg aaggatatat tgctttcctt gatccgccaa aagagacaac
    14641 cgctccggca ctgaaggcat taaaagcgag tggaattacc gtaaaaatcc ttactggcga
    14701 cagtgagtta gttgcagcga aagtgtgcca tgaagtgggg ctggatgcgg gagaggtggt
    14761 cattggtagt gatattgaaa cgctatctga cgatgaactg gcaaatctcg cacagcgcac
    14821 cacgctgttt gcccgcctga cgccgatgca taaagaacgc attgtgactt tactgaagcg
    14881 cgaagggcat gtggttggct ttatgggcga tggtattaat gatgcgcccg ccttacgcgc
    14941 tgcggatatc ggcatttctg tggacggcgc ggtagatatt gcccgtgaag cggctgatat
    15001 catcctgctg gaaaaaagcc tgatggtgct ggaagagggg gttattgagg gacgtcgcac
    15061 tttcgccaac atgctgaaat acatcaaatt gacggcgagt tctaacttcg gtaatgtgtt
    15121 cagcgtgctg gtggcgagtg ctttcttgcc cttcctgccg atgttgccgt tacacttgct
    15181 tattcagaac ctgctgtacg atgtgtcaca ggtggcgatc ccgtttgata acgtcgacga
    15241 cgagcaaatt caaaagccgc agcgttggaa tccggcggat ctggggcgct ttatggtctt
    15301 cttcggaccg atcagctcga ttttcgatat tctgacgttt tgcttgatgt ggtgggtatt
    15361 ccatgccaac acgccggaaa cgcaaacgct gttccagtcg ggctggtttg tggtgggatt
    15421 actgtcgcaa acgctgattg tgcatatgat ccgtacccgc cgtgtgccgt ttattcagag
    15481 ctgtgcatcg tggccgttaa tgatcatgac cgtgatcgtg atgattgtcg ggatcgcatt
    15541 gccgttttca ccgctggcca gttatctgca gctgcaggcg ctgccgttaa gctatttccc
    15601 gtggctggtt gcgattctgg cagggtatat gacgttaacc cagttggtga aagggttcta
    15661 tagccgtcgt tacggctggc agtaaagaat aaactgggca tggtggcccg gattcagatt
    15721 gcagacaaag tgagctttgt tcatgccgga tgcggcgtaa acgcccaatc cggcctacaa
    15781 attcaataaa ttgcaggaat cacgtaggcc tgataagcgt agcgcatcgg gcgattttgc
    15841 ttttgtcatc agacttaatc cgggcatgat agcccggatt tccatcaaga ttagcgacga
    15901 acagcgattg cttcgatctc aatcttcacg tctttcggca gacgggcaac ttcaacgcaa
    15961 gaacgtgccg ggaaagtggc gttgtgttcg gtgaagaagg cttcgtaagt ggcgtttacg
    16021 gttgcgaagt cgttcagatc tttcacgaac acagtggttt taacgatgtc gcccactttc
    16081 aggccagcgg cttcgacgat tgcttttacg ttatccagcg actgacgtgc ctgtgcagcg
    16141 acgtctgccg gtacttcacc agttttcgga tttaccggga tctgaccgga ggtgatgatc
    16201 atattgccca gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc attttccgtc
    16261 gcgatagttt tgctcatgat ttctccttta ttacagcggt aaaaagtctg gctcattata
    16321 gggagccaga cttttattac caaccgcaat taattggcca gcaccacatt atgggaaaac
    16381 tctttttcac agtatttgca tttaagcgcg atgtcattgg cgcgttttcg cacggcaaag
    16441 ctggatgaaa ccggttcggc atggctgata cagttgctgt tcgggcagac cagcacattg
    16501 tcgatgcgct ctggcagact tgggcgcgat ttacccacca cttcatagtt gtcgatacgg
    16561 ttaaccgtgg cttgcggtgc atacaatgcc agttggtcta cttgctcttc actcaaaaag
    16621 gtattttcga ttttgatcag atctttgcgg cccatctcgc cagaaggcaa attcagacca
    16681 atggtgatgc gctgatctgt ttcggtcaac ttgaacagac tcaacagctt aaaaccgatc
    16741 tgggcgggga tatggtcaat taccgtgccg cgtttaatag cttcaacctg caatttatta
    16801 tcgtgtgtca tctctatttc cccttacagt accagatcgc gattcagaac cagtgccagt
    16861 aacgcctggc gagcgaaaat cccgttgcct gcttgctgga agtaccaggc gtgaggcgtt
    16921 ttatccacat ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac tttcatattg
    16981 gctttggcgt tgtggagatc gctggcgcga agaacaaact gcgctttcac gttggcgtac
    17041 tcggacgggt ccagacgctc tttttgcacg cgggtcatgt acaggatgtc tacttccgcc
    17101 atcacttctt caatagagct gtgcagactc caggcgatcc ctttttcatc gagcatatcc
    17161 agaatgtatt gcggcattgc cagcgcatcc ggcgcgataa agtaaaaacg gttgccgtcg
    17221 aacttcgcta gcgcctgggt cagggagtga acggtgcggc catatttcag gtcaccaacc
    17281 attgcgacgt ggagattgtc cagacgcccc tgggtttcct gaatggtgaa taagtccagc
    17341 aaggtttgcg tcggatgttg gttggagcca tcaccggcat tcagtaccgg tacattgcca
    17401 gaaaattcgg tggctaggcg cgccgcacct tcctgcggat gacgcatcac tatcgcatcg
    17461 acgtaagtgc tgataaccga aatggtatcg gccagcgttt cgcctttttt acccagtgat
    17521 gtattggcgc tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat ggaagtttcg
    17581 aaagagaggc gggtacgggt agaggcttcg aagaaacagc tggcaatgac tttgtgcttc
    17641 aacagctctg gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac cagattaagg
    17701 tcatcgcgac taaggtcgtt tatggaaatg atatgtttct gatatagcgg attagccatc
    17761 ttttatctcc tgacacctag gcaaaaaaaa gcccctcgat tgaggggctg ggaatgggtg
    17821 atcaacggga agaaaaacgg caggccagcg tcttttttca gacgcggtaa gacaaaatgt
    17881 cgaacacact gaaccataca tcctcccggc aaattgtccg gcattatact catagccaga
    17941 agcggtgcaa gcatttgatg caatattttg tcagcgcaaa cggtttattt gaattaaaag
    18001 tcaaaatata tgcattttta ttttctatga ttctggaggg gaagtgtcgt tcggtatcaa
    18061 taattgcaga catttcctgc ttttttcttt ttcattttca cgcaatcaga aatgcatttt
    18121 attccattca tccgacttat agggcggagg gtgaaaacga acggctaaca ctattgctta
    18181 tagctcaggg atacgcgcta tcactttaat ttcaaaatca aagcctgcca gccaggtaac
    18241 acccaccgcc gtccagtttg gataaggtgg ggcgctaaat atttcatttt tcaccgtcat
    18301 gatgtcttca aattgttttt ccggatcggt atgaaagctc gtaacatcaa tgatatcgtc
    18361 aaaagtgcat cccgcagctg ccagggtcgc atgcaaatta tcaaaggcca gtctgacttg
    18421 ttgctgaaaa tcgggttctg gtgttccgtc ctctcgactt cctacttgcc cggaaacaaa
    18481 cagcaaatcg ccggaacgaa tagccgcaga ataacgatgc tcagcataca gtgaatgtcg
    18541 gccagcaggg aaaacagcgg ttctttctac catttggtta tcctcaagat ttacgacatg
    18601 aacagaagat ttatatttat cgggagccgc ttttaggggg acgacgttag taaacaaaac
    18661 ccagacatca tggataatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    18721 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    18781 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcaac
    18841 atatcgcgca ttggcccgtt ggcgggatta acgtcggtat caattggtcc tggctggacg
    18901 acgttgatgg tgatccctcg cggtccaaaa tcacgggcca gcccgcgcgc catgccttgc
    18961 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    19021 acggagccga tgattaagat gcgcccgcct tcgggcatct gccgggcggc ttcaacagag
    19081 gcatgataag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    19141 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    19201 ctcttacgaa cgacatcaat aacagcgtct ctgtcagcac tatctgtgaa tactgctgtc
    19261 gctccagtct cttgtgccag acgttcagcg gcatctttcg accccgcata ggtgaatcgt
    19321 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acgactgcca
    19381 ccgaggatga gaactgtctt accggtaaaa gcgcccataa ggactccttg atttattatg
    19441 taacatgcat tacaaaactg ttttaacttt ctgtcaacag gttttgtaat ggtcactaaa
    19501 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    19561 gcaaaagtgc tttttcacca aaaaggtttc gatgcagtaa gtgtctctga agttactgat
    19621 tatcttggta ttaacccccc gagtctctac gcggcttttg gcagtaaggc tgggttattt
    19681 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    19741 gatgatcgtc cggtaggcga gtgcctggtt gaggtattaa aagaagcggc gcgcagatat
    19801 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gtattcatag tcatgatcca
    19861 caagcgcgtg atattgccat tcaatattat cacgccgcag aaacgaccat ttatgactat
    19921 atcgccaagc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    19981 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    20041 gccctggcag gggaagcgat aaaaactctt ctcaaggagt gatgctggcc ttgatccgaa
    20101 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctgg
    20161 cggaattgat catcttttcg tataacgata gaaatgaaac attattttaa ttaaggagtg
    20221 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    20281 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcatgat
    20341 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gtacgaagct
    20401 cgccgtgcgg agtaccatgc ccgctatctt gacattcaga ttgtgttaaa aggtcaggaa
    20461 ggcatgacct tcagcacgca acctgcaggc gcgccggata ccgactggtt agctgataaa
    20521 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatcctaaa tgaaggtgat
    20581 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    20641 caggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    20701 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    20761 ttgtaatgct tagacgttct tattaactca aggagttcgt catgggcaaa atatcagatt
    20821 tgaattattc tcaacacatt
//
LOCUS       DAECNO010000069        20714 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.069, whole genome shotgun sequence.
ACCESSION   DAECNO010000069 DAECNO010000000
VERSION     DAECNO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.069"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="KKO08_002647"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="KKO08_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBD6318842.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="KKO08_002648"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="KKO08_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBD6318843.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="KKO08_002649"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="KKO08_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBD6318844.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="KKO08_002650"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="KKO08_002650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBD6318845.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8241
                     /gene="elyC"
                     /locus_tag="KKO08_002651"
                     /pseudo
     CDS             7461..8241
                     /gene="elyC"
                     /locus_tag="KKO08_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
     gene            complement(8218..9111)
                     /locus_tag="KKO08_002652"
     CDS             complement(8218..9111)
                     /locus_tag="KKO08_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBD6318846.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="KKO08_002653"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="KKO08_002653"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBD6318847.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="KKO08_002654"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="KKO08_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBD6318848.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="KKO08_002655"
     CDS             complement(10242..11474)
                     /locus_tag="KKO08_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBD6318849.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="KKO08_002656"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="KKO08_002656"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBD6318850.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="KKO08_002657"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="KKO08_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBD6318851.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="KKO08_002658"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="KKO08_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="KKO08_002659"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="KKO08_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBD6318852.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="KKO08_002660"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="KKO08_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBD6318853.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="KKO08_002661"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="KKO08_002661"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBD6318854.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="KKO08_002662"
     CDS             complement(19833..20594)
                     /gene="ycaL"
                     /locus_tag="KKO08_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBD6318855.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4983 a         5726 c         5032 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgcaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa caacgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttga ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcta ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tagcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttagccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccaggctag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaagagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacgaacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat gtgc
//
LOCUS       DAECNO010000070        20699 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.070, whole genome shotgun sequence.
ACCESSION   DAECNO010000070 DAECNO010000000
VERSION     DAECNO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.070"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            81..350
                     /locus_tag="KKO08_002663"
     CDS             81..350
                     /locus_tag="KKO08_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBD6318856.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="KKO08_002664"
     CDS             344..709
                     /locus_tag="KKO08_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBD6318857.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KKO08_002665"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="KKO08_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBD6318858.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="KKO08_002666"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="KKO08_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBD6318859.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="KKO08_002667"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="KKO08_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBD6318860.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="KKO08_002668"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="KKO08_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBD6318861.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KKO08_002669"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="KKO08_002669"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBD6318862.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KKO08_002670"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="KKO08_002670"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBD6318863.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="KKO08_002671"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="KKO08_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KKO08_002672"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="KKO08_002672"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBD6318864.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KKO08_002673"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="KKO08_002673"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBD6318865.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14857..16455
                     /gene="rtcR"
                     /locus_tag="KKO08_002674"
     CDS             14857..16455
                     /gene="rtcR"
                     /locus_tag="KKO08_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBD6318866.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16437..17195)
                     /locus_tag="KKO08_002675"
     CDS             complement(16437..17195)
                     /locus_tag="KKO08_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBD6318867.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="KKO08_002676"
     CDS             complement(17212..18042)
                     /gene="glpG"
                     /locus_tag="KKO08_002676"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBD6318868.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="KKO08_002677"
     CDS             complement(18087..18413)
                     /gene="glpE"
                     /locus_tag="KKO08_002677"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBD6318869.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18603..20108
                     /gene="glpD"
                     /locus_tag="KKO08_002678"
     CDS             18603..20108
                     /gene="glpD"
                     /locus_tag="KKO08_002678"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBD6318870.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20377..20607)
                     /locus_tag="KKO08_002679"
                     /pseudo
     CDS             complement(20377..20607)
                     /locus_tag="KKO08_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5692 c         5219 g         4899 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg caccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttttt aatttattgt
    14761 ttctaaaaag attatatctt tatgtccgta aacggagatt tcccgcaaag ccgatttacc
    14821 gataatgaaa tatcgccttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    14881 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc aaaatggcgt
    14941 ccgtcactct gtatatacca gcaagaatcg ttggtcatcg atcgactgga attattgcac
    15001 gatgcccgct cgcgctcgct atttgaaacg cttaaacgcg atatcgccag cgtttcgcca
    15061 gaaacagaag tggtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    15121 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    15181 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    15241 gcgcgttatc tacccgcccg gttgatacaa tcttcaactc cgcgcaaaaa agaacagccg
    15301 cgcggcccag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    15361 agccgctttg ccgaggaacg gcagcaaacg ctcgatttcc tcaaatccgg tattgccacg
    15421 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat taaatcccgc
    15481 gcgccgattc tgcttaacgg cccgaccggc gcaggtaagt catttctggc gcgacgcatc
    15541 ttcgagttaa aacaggcacg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    15601 accctgcgcg gcgataccgc gatgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc
    15661 ggggcgcggg aatctcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt
    15721 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc tattgaagag
    15781 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    15841 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aattccgcga agatctgtac
    15901 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    15961 gaaccgaacc tggattatga agtggagcgc cacgccacac tcactggtga cagcgtgcgt
    16021 tttaacaccg aagcgcggcg tgcctggctg gcctttgcaa catctcccca ggcaacatgg
    16081 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    16141 ggacgcatca ctctggacgt ggtggaagat gagataaacc gtctgcgcta taactggcag
    16201 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
    16261 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttct
    16321 gctgccggac gacagctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    16381 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    16441 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
    16501 gcgtcggtgt agacggcatc aaccatgctg atgctcccca tattgaccat cgcattacgg
    16561 ccaaattttg aatggtcaac caccagcata acgtggcgcg agttctcaat aatggcgcgc
    16621 ttggtacgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctgtc gatgccgctg
    16681 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    16741 atgatcccgc catcgcggct acgtaattcg ccaccggcga gaataatgcg gaaatcttct
    16801 tttaccatca acgtgttagc aacgttgaga ttgttggtga caatgcgcaa attgctgtga
    16861 ttgagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagcgtcgag
    16921 ccattgggga tttgctccgc cactttgcgg gcgatgcgct ctttttcttc ggtctgggtg
    16981 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc gccgccatga
    17041 tggcgcagga tcaggttttg ctctgccagc tcgttgaggt cgcggcgaat agtctgcggg
    17101 ctgacggaga agtgctccac cagttcctcg gtactgacat aaccctgctg tttaaccagt
    17161 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    17221 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta atccggcgat
    17281 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    17341 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
    17401 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    17461 cccgctgaat ttttgctgca tatagccgct taacagggcg ctgataagcg taatgacaat
    17521 tagcttaccg ctaccgaggc gtttttccac cgcgccgccg agataccacc accaaagcag
    17581 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    17641 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
    17701 gagaatttgc atggcaataa acaccaccac gcaggcgatc atcatcaccc aggtcaccgg
    17761 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    17821 ggtatggcct gccagccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    17881 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
    17941 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    18001 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
    18061 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactgt
    18121 cgctgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    18181 agatactgcg ccgccccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    18241 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    18301 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    18361 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    18421 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    18481 acgccataat gttatacata tcactctaaa atgttttttc aatgttaccc aaagcgcgat
    18541 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    18601 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    18661 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    18721 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    18781 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    18841 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    18901 tgattcgcat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    18961 caactggttt gcgttttggc gcaaattcag tgttaaaacc ggaaattaag cgcggattcg
    19021 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    19081 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    19141 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    19201 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    19261 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgcgtgc
    19321 atacccaaaa gcaggcctac attctgcaaa acgaagataa acgcattgtg ttcgtgatcc
    19381 cgtggatgga cgagttttcc atcatcggca ccaccgatgt ggaatacaaa ggtgatccga
    19441 aagcagtgaa gattgaagag agtgaaatca attacctgct gaaagtgtat aacgcgcact
    19501 ttaaaaagca gctaagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    19561 gtgatgatga gtccgactcg ccgcaagcca ttacccgtga ttacaccctt gatatccatg
    19621 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    19681 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    19741 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgccg
    19801 ctcgcctgcg ccgccgttac ccgttcctga ctgaatcgct ggcacgtcac tacacgcgca
    19861 cttacggcag caacagcgag ctgctgctcg gcaatgcggg aacggtaagc gatctcgggg
    19921 aagatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    19981 tccgccgcac cgacgatgcc ctgtggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    20041 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    20101 cgtcgtaaat taacgtaagg tgatcaggtc agatttcaat ctggcctgag actgatgaca
    20161 aacacaaaac tgcctgatgc gctacgctta tcaggcctac gtggtttatg caatatattg
    20221 aatttgcatg gtcttgtagg ccagataaga cgttcacgtc gcatccggca tgaacaaagc
    20281 gcactttgtc aaaaatctaa cctacttttt aattcaggga attaccgcaa agcccacgcc
    20341 atcataccca acgtaacaaa actcaggcac gttccccact cgccccgaga aaaatagcat
    20401 taatgcgccc agcgtcagca taaaaatatt gagcagaagg taaattgttg gcgtaaagag
    20461 taaattgcaa acctcaggaa tagcggcaag cccagccccc agcatggcaa taaccgctgc
    20521 aaaaaattgc gtcagtattg ggatgccgaa aaaagtcatt accagcgagg tcaaaatcca
    20581 ttttctattt tgcattattc tttccattct ttctgaatgg cgaaattata ctccccgagt
    20641 ccccttgccc cttctggaca cttttccgaa atgatggcga aaaaaacggg accctttgg
//
LOCUS       DAECNO010000071        20620 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.071, whole genome shotgun sequence.
ACCESSION   DAECNO010000071 DAECNO010000000
VERSION     DAECNO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.071"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            98..397
                     /locus_tag="KKO08_002680"
     CDS             98..397
                     /locus_tag="KKO08_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6318871.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            407..931
                     /gene="ygaP"
                     /locus_tag="KKO08_002681"
     CDS             407..931
                     /gene="ygaP"
                     /locus_tag="KKO08_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBD6318872.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(978..1382)
                     /gene="stpA"
                     /locus_tag="KKO08_002682"
     CDS             complement(978..1382)
                     /gene="stpA"
                     /locus_tag="KKO08_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBD6318873.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2050..2499
                     /gene="alaE"
                     /locus_tag="KKO08_002683"
     CDS             2050..2499
                     /gene="alaE"
                     /locus_tag="KKO08_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBD6318874.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2536..2880)
                     /locus_tag="KKO08_002684"
     CDS             complement(2536..2880)
                     /locus_tag="KKO08_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBD6318875.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3032..3262
                     /locus_tag="KKO08_002685"
     CDS             3032..3262
                     /locus_tag="KKO08_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318876.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3609..3854
                     /gene="nrdH"
                     /locus_tag="KKO08_002686"
     CDS             3609..3854
                     /gene="nrdH"
                     /locus_tag="KKO08_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBD6318877.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3851..4261
                     /gene="nrdI"
                     /locus_tag="KKO08_002687"
     CDS             3851..4261
                     /gene="nrdI"
                     /locus_tag="KKO08_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBD6318878.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4234..6378
                     /gene="nrdE"
                     /locus_tag="KKO08_002688"
     CDS             4234..6378
                     /gene="nrdE"
                     /locus_tag="KKO08_002688"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBD6318879.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6388..7347
                     /gene="nrdF"
                     /locus_tag="KKO08_002689"
     CDS             6388..7347
                     /gene="nrdF"
                     /locus_tag="KKO08_002689"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBD6318880.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7702..8904
                     /gene="proV"
                     /locus_tag="KKO08_002690"
     CDS             7702..8904
                     /gene="proV"
                     /locus_tag="KKO08_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBD6318881.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8897..9961
                     /gene="proW"
                     /locus_tag="KKO08_002691"
     CDS             8897..9961
                     /gene="proW"
                     /locus_tag="KKO08_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBD6318882.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10019..11011
                     /gene="proX"
                     /locus_tag="KKO08_002692"
     CDS             10019..11011
                     /gene="proX"
                     /locus_tag="KKO08_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBD6318883.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11203..12382
                     /locus_tag="KKO08_002693"
                     /pseudo
     CDS             11203..12382
                     /locus_tag="KKO08_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12506..13243
                     /gene="ygaZ"
                     /locus_tag="KKO08_002694"
     CDS             12506..13243
                     /gene="ygaZ"
                     /locus_tag="KKO08_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBD6318884.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13233..13568
                     /gene="ygaH"
                     /locus_tag="KKO08_002695"
     CDS             13233..13568
                     /gene="ygaH"
                     /locus_tag="KKO08_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBD6318885.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13659..14189
                     /gene="emrR"
                     /locus_tag="KKO08_002696"
     CDS             13659..14189
                     /gene="emrR"
                     /locus_tag="KKO08_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBD6318886.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14316..15488
                     /gene="emrA"
                     /locus_tag="KKO08_002697"
                     /pseudo
     CDS             14316..15488
                     /gene="emrA"
                     /locus_tag="KKO08_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15505..17043
                     /gene="emrB"
                     /locus_tag="KKO08_002698"
     CDS             15505..17043
                     /gene="emrB"
                     /locus_tag="KKO08_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBD6318887.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="KKO08_002699"
     CDS             complement(17107..17622)
                     /gene="luxS"
                     /locus_tag="KKO08_002699"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBD6318888.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="KKO08_002700"
     CDS             complement(17772..19328)
                     /gene="gshA"
                     /locus_tag="KKO08_002700"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBD6318889.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19401..19829)
                     /locus_tag="KKO08_002701"
     CDS             complement(19401..19829)
                     /locus_tag="KKO08_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6318890.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="KKO08_002702"
     CDS             complement(19826..20392)
                     /gene="yqaB"
                     /locus_tag="KKO08_002702"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBD6318891.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20540..20616)
                     /locus_tag="KKO08_002703"
     tRNA            complement(20540..20616)
                     /locus_tag="KKO08_002703"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20580..20582),aa:Arg,seq:acg)
BASE COUNT         4971 a         5278 c         5152 g         5219 t
ORIGIN      
        1 tcagcttacg cttttactct catttacgct gctgaggctg gtatatgttg catttttagt
       61 ttagagttta cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca
      121 ggccagtacc gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct
      181 gattctgtgc atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac
      241 cggactgagc gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat
      301 caacagccaa cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc
      361 cattatcgcc accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac
      421 catttcgccg catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg
      481 tgacgctgat gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt
      541 gctggaacag tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca
      601 ggcaggtaaa cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga
      661 aatattttta ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt
      721 aaataaatct caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat
      781 attaatcggc gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt
      841 tgttggtgcc gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt
      901 agataagatg ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg
      961 acatccggcc tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc
     1021 tgagcaattg gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca
     1081 tcagtgaatt tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca
     1141 gcagagctat tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc
     1201 caggtgctaa ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca
     1261 cgtctttctt tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag
     1321 aattcgcgag ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac
     1381 ataataaaga aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg
     1441 atcacttatt atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca
     1501 gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga
     1561 gtaatttagg ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga
     1621 caaaaccgaa gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt
     1681 gctttctgct gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcacg
     1741 acaaacaccg aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa
     1801 attaaaggca agatcatatc taattgattt aattgaaaat aaatcatatc ggcatgtaaa
     1861 aatccacaat gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa
     1921 ataaaaattt ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca
     1981 gatggcaatg agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg
     2041 agcattagca tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg
     2101 atggttgttt actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc
     2161 ttcgaacagt ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg
     2221 ccatacggta tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc
     2281 tggataaaaa atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg
     2341 gcgatcttgt tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac
     2401 atcgttgttt cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga
     2461 ctgtttaaag tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca
     2521 tcgccagtcg cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc
     2581 aacacgcgaa ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata
     2641 gagcggaaat tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt
     2701 tgaactgcgt tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc
     2761 gcgattaaca tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac
     2821 atcgacagta aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat
     2881 atttgatcct cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc
     2941 tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg
     3001 aaaattacat cgaattctaa tgggggatca tatgtataac cgaccgaacc gcaacgacgt
     3061 tgatgatggc gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc
     3121 tgtattgaag tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc
     3181 tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc
     3241 cgcgcgcgat gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt
     3301 gggtacagca gctgcggtag gtatttttat cggcgcactg ttaagcgtgc gcaaatcgta
     3361 gtgcaaaaat gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt
     3421 ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg
     3481 atcactattt gctatatatt gtgtggttga atcttttttc aactacatct agtatctctg
     3541 tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa
     3601 tacgaatcat gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca
     3661 aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg
     3721 aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg
     3781 atcttagctg gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg
     3841 cggccagtgc atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt
     3901 tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca
     3961 ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc
     4021 ggtgccacga caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg
     4081 cgttattgct tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat
     4141 tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga
     4201 tatcgaaaac gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg
     4261 acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca
     4321 ggtcgcattc agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg
     4381 cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa
     4441 ggttactatg atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt
     4501 gcccacgcac acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac
     4561 accagctata cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga
     4621 gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc
     4681 gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag
     4741 cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg
     4801 attggtcggg cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt
     4861 ttgctgtcga atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct
     4921 ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc
     4981 gcgcgtcagg gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt
     5041 ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc
     5101 gtggtgatcc cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt
     5161 tcgccctatg acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa
     5221 cactatgacg aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat
     5281 ttcttccagc gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa
     5341 gacacagtaa accgtgctag ccctatcgcc gggcgcataa atatgagtaa tctctgctca
     5401 gaaattttgc aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca
     5461 ggccatgata tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc
     5521 gatttttccc gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt
     5581 catatccgca gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg
     5641 gggcagatga atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa
     5701 gccctggatt tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg
     5761 atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc
     5821 agtggtgaat atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt
     5881 ggcgaactgt ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg
     5941 cgcgacgacg tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc
     6001 gggtctatct cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta
     6061 gagatacgca aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac
     6121 gaaaatctgg cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc
     6181 tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat
     6241 accgccacca ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa
     6301 acgctctatt acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc
     6361 gtctcctgtg cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg
     6421 aacaagatat ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg
     6481 ctaccagaaa aggtgccgct gtcgaacgat attcccgcct ggcagacatt aactgtcgta
     6541 gaacaacaac tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat
     6601 gttatcggcg cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta
     6661 tcgaatatca gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg
     6721 ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg
     6781 cagcgaaaag ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa
     6841 atcgccagtg tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat
     6901 ttttccagcc gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat
     6961 gaagcagtcc acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct
     7021 ctgggacaac gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac
     7081 aacgagttgc aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa
     7141 gcgtttctct gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt
     7201 cccgcagaaa tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat
     7261 gaaaatcacg atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa
     7321 acagaagatg aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag
     7381 cagggaaata attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca
     7441 ctgatactct gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct
     7501 atgacaaaaa atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc
     7561 ggcgatttgc tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct
     7621 cagtatgtta gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa
     7681 ataaaggaat cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata
     7741 tttggtgagc atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa
     7801 attctggaaa aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa
     7861 ggcgagatat ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt
     7921 ctcaatcgcc tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc
     7981 aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag
     8041 tcctttgcct taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg
     8101 gccggaatta atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg
     8161 ctggaaaatt atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg
     8221 ggattagccc gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg
     8281 gcgctcgatc cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa
     8341 catcagcgca ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac
     8401 cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc
     8461 aataatccgg cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg
     8521 ttcagtgcga aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc
     8581 ttcggcccac gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt
     8641 atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta
     8701 acgcagcagc aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa
     8761 acgcctctta gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc
     8821 gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat
     8881 cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga
     8941 cagtgccgcg caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg
     9001 tggtgctgac tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga
     9061 tccgttccat aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt
     9121 tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa
     9181 cggtttccag caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct
     9241 catcacctgg cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc
     9301 catcggcgca atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc
     9361 cctgctgttc tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg
     9421 agcggcgaaa attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta
     9481 tctggtgcca atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat
     9541 catctttgcg ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc
     9601 ggatctgatt gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt
     9661 tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc
     9721 cctttctatg gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact
     9781 tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat
     9841 cctcgccatt atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg
     9901 caaccgtcgc tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta
     9961 actctgcact tgcccggtgg cgccgggcat tatcaccctg ccaaaaaaag gaataacaat
    10021 gcgacatagc gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc
    10081 tgccgatctg ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga
    10141 aaccttccag acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa
    10201 acccagcgaa gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt
    10261 caccgccgtg aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa
    10321 gaaattttat cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa
    10381 gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc
    10441 caaactgttc gataccaacg gcgacggtaa agcggattta accggttgta accctggctg
    10501 gggctgcgaa ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac
    10561 tcataatcag gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg
    10621 caaaccggtg ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg
    10681 gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga
    10741 taccaaactg ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc
    10801 caacaaagcc tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt
    10861 gccagtggcc gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg
    10921 cgatattcag ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg
    10981 ggtgaatgag gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg
    11041 cctcatccgc cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta
    11101 caaaccacat cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta
    11161 ttattgattt ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat
    11221 gagcttagcc cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt
    11281 aactattacg cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt
    11341 tcggcaggct ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt
    11401 gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca
    11461 gcgggcggta tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg
    11521 gcattaaccg gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg
    11581 gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg
    11641 atattgctgg cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc
    11701 ttttgggttg cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca
    11761 caaatgaaat cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg
    11821 ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac
    11881 ttcagcattc tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc
    11941 gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg
    12001 gcgggcggtt ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt
    12061 actttcatgg ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat
    12121 tctggtgctg gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg
    12181 aatacatcct gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg
    12241 cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt
    12301 ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca
    12361 tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag
    12421 ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct atttataata
    12481 ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc
    12541 ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt
    12601 ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt
    12661 tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc
    12721 cgggagtagt ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt
    12781 gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct
    12841 atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa
    12901 taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg
    12961 ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc
    13021 cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc
    13081 tttccagcgc aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt
    13141 aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc
    13201 gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt
    13261 tactggttgg cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta
    13321 atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct
    13381 cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt
    13441 tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca
    13501 ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga
    13561 ttatataacc cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc
    13621 gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca
    13681 ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc
    13741 ttctgactcg tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc
    13801 tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg
    13861 aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg
    13921 cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata
    13981 acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg
    14041 ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa
    14101 aagatcagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag
    14161 acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat
    14221 aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc
    14281 tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa
    14341 ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct
    14401 tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt
    14461 tcgaagaaac cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt
    14521 ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc
    14581 tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg
    14641 cttccagcgt tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata
    14701 ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc
    14761 tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca
    14821 gtgcccaggc gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc
    14881 tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg
    14941 cctagctggc gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc
    15001 gtgccgtgca accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag
    15061 ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg
    15121 gtcagccggt caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag
    15181 tggttggtct ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga
    15241 ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac
    15301 agctggagca atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta
    15361 accgtgacgg tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg
    15421 cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg
    15481 ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg
    15541 gtcattatga cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt
    15601 gctaacgtgg cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg
    15661 tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg
    15721 gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg
    15781 tcttgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag
    15841 gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg
    15901 ccagccaaac gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt
    15961 tgcggcccga tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc
    16021 atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt
    16081 gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt
    16141 atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag
    16201 gaaattatca ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag
    16261 ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc
    16321 ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg acgctattgt tctgctgccg
    16381 cagttgttgc aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg
    16441 gtagggatta ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat
    16501 atgcggcggc tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc
    16561 tatacctttg aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg
    16621 tttgcggtgg tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg
    16681 gaacgactgg cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc
    16741 ggcacgtcga taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg
    16801 actgagtcgg taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg
    16861 cttgggatga cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg
    16921 ctgattattt ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg
    16981 gggctggtgt ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac
    17041 taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt
    17101 tactgactag atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct
    17161 gttgatgcgt acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga
    17221 gtgcatctgg taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg
    17281 cactttcagc acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg
    17341 cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat
    17401 ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt
    17461 atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac
    17521 ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc
    17581 catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat
    17641 ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg
    17701 catttgttat catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt
    17761 tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt
    17821 tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc
    17881 agaatttcca gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct
    17941 gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg
    18001 ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta
    18061 atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc
    18121 agtaacggga actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct
    18181 tcaaggatca cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc
    18241 ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca
    18301 tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg
    18361 ccgccacgta acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga
    18421 atcggcgcat acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta
    18481 ccgtctttct caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta
    18541 aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta
    18601 ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg
    18661 cactcggttt tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc
    18721 gccggagatg caccaaacag ataaggaatg acccaaccga aacgatagta attgcggata
    18781 acgcggaaat agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac
    18841 ttcgcttgcc agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc
    18901 atcagcgcgc cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg
    18961 ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata
    19021 cttaacggcc acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc
    19081 ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct
    19141 tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga
    19201 cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc
    19261 tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc
    19321 gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga
    19381 ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca
    19441 actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag
    19501 atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat
    19561 aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat
    19621 gttttacgca atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta
    19681 cccattgttg ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc
    19741 accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac
    19801 aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg
    19861 ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt
    19921 gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga
    19981 cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg
    20041 cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac
    20101 ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg
    20161 cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa
    20221 ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt
    20281 actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggctccgtat
    20341 ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct
    20401 cgcaagtcga aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg
    20461 aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa
    20521 ggaaattttt tggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    20581 gtagccgagt actctatcca gctgagctac ggatacatcg
//
LOCUS       DAECNO010000072        20523 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.072, whole genome shotgun sequence.
ACCESSION   DAECNO010000072 DAECNO010000000
VERSION     DAECNO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.072"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="KKO08_002704"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="KKO08_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBD6318892.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="KKO08_002705"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="KKO08_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBD6318893.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="KKO08_002706"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="KKO08_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBD6318894.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="KKO08_002707"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="KKO08_002707"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBD6318895.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="KKO08_002708"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="KKO08_002708"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBD6318896.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="KKO08_002709"
     CDS             4451..4816
                     /locus_tag="KKO08_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBD6318897.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="KKO08_002710"
     CDS             4966..5484
                     /locus_tag="KKO08_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBD6318898.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="KKO08_002711"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="KKO08_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="KKO08_002712"
     CDS             6715..7197
                     /locus_tag="KKO08_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBD6318899.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="KKO08_002713"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="KKO08_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBD6318900.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="KKO08_002714"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="KKO08_002714"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBD6318901.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="KKO08_002715"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="KKO08_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBD6318902.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="KKO08_002716"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="KKO08_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBD6318903.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="KKO08_002717"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="KKO08_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBD6318904.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="KKO08_002718"
     CDS             10941..11732
                     /locus_tag="KKO08_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBD6318905.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="KKO08_002719"
     CDS             11753..13039
                     /locus_tag="KKO08_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBD6318906.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="KKO08_002720"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="KKO08_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBD6318907.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="KKO08_002721"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="KKO08_002721"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBD6318908.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="KKO08_002722"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="KKO08_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBD6318909.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="KKO08_002723"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="KKO08_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBD6318910.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="KKO08_002724"
     CDS             complement(16987..17277)
                     /locus_tag="KKO08_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBD6318911.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="KKO08_002725"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="KKO08_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBD6318912.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="KKO08_002726"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="KKO08_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBD6318913.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="KKO08_002727"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="KKO08_002727"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="KKO08_002728"
     CDS             19139..20380
                     /locus_tag="KKO08_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD6318914.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5303 a         5360 c         4814 g         5046 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctag aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacatg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacagat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatctgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacagacaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacattccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttacc tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattacaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aggaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtc ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgagc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cctatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DAECNO010000073        20417 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.073, whole genome shotgun sequence.
ACCESSION   DAECNO010000073 DAECNO010000000
VERSION     DAECNO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.073"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="KKO08_002729"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="KKO08_002729"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBD6318915.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="KKO08_002730"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="KKO08_002730"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBD6318916.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2998)
                     /locus_tag="KKO08_002731"
     CDS             complement(1727..2998)
                     /locus_tag="KKO08_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6318917.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVQFGERKFSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="KKO08_002732"
     CDS             complement(3181..4386)
                     /locus_tag="KKO08_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6318918.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="KKO08_002733"
     CDS             complement(4486..4842)
                     /locus_tag="KKO08_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBD6318919.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="KKO08_002734"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="KKO08_002734"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="KKO08_002735"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="KKO08_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBD6318920.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="KKO08_002736"
     CDS             6772..9828
                     /locus_tag="KKO08_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD6318921.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="KKO08_002737"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="KKO08_002737"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBD6318922.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="KKO08_002738"
     CDS             10335..10523
                     /locus_tag="KKO08_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBD6318923.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="KKO08_002739"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="KKO08_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBD6318924.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="KKO08_002740"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="KKO08_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBD6318925.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="KKO08_002741"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="KKO08_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBD6318926.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="KKO08_002742"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="KKO08_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBD6318927.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="KKO08_002743"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="KKO08_002743"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBD6318928.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="KKO08_002744"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="KKO08_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBD6318929.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="KKO08_002745"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="KKO08_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBD6318930.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="KKO08_002746"
     CDS             complement(17799..18035)
                     /gene="lpp"
                     /locus_tag="KKO08_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBD6318931.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="KKO08_002747"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="KKO08_002747"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBD6318932.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(20307..>20417)
                     /locus_tag="KKO08_002748"
     CDS             complement(20307..>20417)
                     /locus_tag="KKO08_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6318933.1"
                     /translation="LRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         5344 a         4888 c         5204 g         4981 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgatagt tggtaatgac tccaacttac tgatagtgtt ttatgttcag ataatgcccg
    20341 atgaccttgt catgcagctc caccgatttt gagaacgaca gtgacttccg tcccagcctt
    20401 gccagatgtt gtctcag
//
LOCUS       DAECNO010000074        20242 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.074, whole genome shotgun sequence.
ACCESSION   DAECNO010000074 DAECNO010000000
VERSION     DAECNO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.074"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            81..911
                     /locus_tag="KKO08_002749"
     CDS             81..911
                     /locus_tag="KKO08_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD6318934.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1052..1825)
                     /gene="uxuR"
                     /locus_tag="KKO08_002750"
     CDS             complement(1052..1825)
                     /gene="uxuR"
                     /locus_tag="KKO08_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBD6318935.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2040..3500)
                     /locus_tag="KKO08_002751"
     CDS             complement(2040..3500)
                     /locus_tag="KKO08_002751"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBD6318936.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3581..4765)
                     /gene="uxuA"
                     /locus_tag="KKO08_002752"
     CDS             complement(3581..4765)
                     /gene="uxuA"
                     /locus_tag="KKO08_002752"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBD6318937.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5105..6448
                     /gene="gntP"
                     /locus_tag="KKO08_002753"
     CDS             5105..6448
                     /gene="gntP"
                     /locus_tag="KKO08_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBD6318938.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6699..7601)
                     /gene="fimH"
                     /locus_tag="KKO08_002754"
     CDS             complement(6699..7601)
                     /gene="fimH"
                     /locus_tag="KKO08_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBD6318939.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7621..8124)
                     /gene="fimG"
                     /locus_tag="KKO08_002755"
     CDS             complement(7621..8124)
                     /gene="fimG"
                     /locus_tag="KKO08_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBD6318940.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8137..8667)
                     /gene="fimF"
                     /locus_tag="KKO08_002756"
     CDS             complement(8137..8667)
                     /gene="fimF"
                     /locus_tag="KKO08_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBD6318941.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8677..11313)
                     /gene="fimD"
                     /locus_tag="KKO08_002757"
     CDS             complement(8677..11313)
                     /gene="fimD"
                     /locus_tag="KKO08_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBD6318942.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11380..12105)
                     /gene="fimC"
                     /locus_tag="KKO08_002758"
                     /pseudo
     CDS             complement(11380..12105)
                     /gene="fimC"
                     /locus_tag="KKO08_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12142..12681)
                     /locus_tag="KKO08_002759"
     CDS             complement(12142..12681)
                     /locus_tag="KKO08_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6318943.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKWLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12746..13294)
                     /gene="fimA"
                     /locus_tag="KKO08_002760"
     CDS             complement(12746..13294)
                     /gene="fimA"
                     /locus_tag="KKO08_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBD6318944.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(13775..14371)
                     /gene="fimE"
                     /locus_tag="KKO08_002761"
     CDS             complement(13775..14371)
                     /gene="fimE"
                     /locus_tag="KKO08_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBD6318945.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(14849..15451)
                     /gene="fimB"
                     /locus_tag="KKO08_002762"
                     /pseudo
     CDS             complement(14849..15451)
                     /gene="fimB"
                     /locus_tag="KKO08_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            16905..17621
                     /gene="nanC"
                     /locus_tag="KKO08_002763"
     CDS             16905..17621
                     /gene="nanC"
                     /locus_tag="KKO08_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBD6318946.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17641..18747
                     /gene="nanM"
                     /locus_tag="KKO08_002764"
     CDS             17641..18747
                     /gene="nanM"
                     /locus_tag="KKO08_002764"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBD6318947.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            18812..19792
                     /locus_tag="KKO08_002765"
     CDS             18812..19792
                     /locus_tag="KKO08_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBD6318948.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5357 a         4857 c         4630 g         5398 t
ORIGIN      
        1 ttgtttactt ctctttttac tacagaaaaa tttatctatt tttctaaaca tcagttaaat
       61 actcataaaa ggtaactatt atgtctatgc cattaagcaa tgccgctaat aaccaattca
      121 atacaaataa tcacttcctt catcatccaa aagtcgacag tgagttaaca cgtcgatatg
      181 aatatgcacg cctggatacg gaaaatatct atcttctgcc tcttgctcgt ggcaataacc
      241 ataattacga tggaaaaagc gttgtcgaaa ttagaaaact taatattggc aaacagtcct
      301 ggccatttaa ttatgtgaca gatacatgca gagaattcaa tggtattaca acaacaggac
      361 gtatgctcta tcgaaacttg aaaatcacct cagctttaga cgaaatatat ggtggtatat
      421 gtaaaaaggc gcatgctgct acagagttag cagaaggttt acggctaaat ttatttatga
      481 agagcccttt cgatcctgtt gaggactata cagttcatga aataacatta ggccctggtt
      541 gcaatgttcc tggttatgcc ggaaccacga taggatatat ttcaaccctt ccagcttctc
      601 aggcaaaaag atggaccaat gagcagccaa gaattgatat ctatattgat cagataataa
      661 ctgtctcagg ggtagcaaat tcttccggat tcgcccttgc cgcattactt aatgctaata
      721 ttgagatggg aaatgaccca atcattggta tagaagccta tcctggtacc gcggagatcc
      781 attcaaaaat gggatataac gttatccccg gtgatgaaga tgcgccactc aaacgcatga
      841 ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa tggaattata
      901 ttggtaaata gccactgaga agaatgagta ctaatattca ggttatacag atatttaaac
      961 aaaaccctgc aatttacaaa gtaatactca ttgcgacttc ttaatttaag aaagcgcaag
     1021 gaacgtttac ccttgcgctt attataataa gtcaggcgtc gactttatcc agcggccatg
     1081 aatcaaagag atagccgtca aaataaatat cgtcaacgtt cgagaattcc agcagacgtt
     1141 gcttaacgtt ttccagatgc tgccacattg ccagcttcgc cgctcgggca tcttttttga
     1201 ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat
     1261 ccagatggct gtgcaactgg atccacattg gattgttttc ccgccactgc caggactgac
     1321 ggaataactc caccagcatg ctgttatgcg ttgcttcagc aatggcgaga tggaactgca
     1381 tgtcaccgct ttcgctgcta cccggcgcgc tggaagccag ttcacgctct tccagttgca
     1441 atgcctgacg cattttgacg atatcttcgc gggtagcctg cagtgcggca aactcggcga
     1501 tgttactttc cagtaactga cgcgcctgta acagctcaaa aggacccgca tcattgcaga
     1561 cgttggcatc cggactgtct gtgttgtggc tgcctgagct atcaagcaca tagataccgg
     1621 caccccggcg cacttccacc agccctttga tctccagcat gatcagcgct tcacgtacca
     1681 ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggcggcagc cgttcgccag
     1741 gattgtacgg cgtctttatg atcagatcgc ggatcatcgc cccgacttcc tggtaaggtc
     1801 tttgcgcaga ggtgacagat ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt
     1861 ttgccggatg cggcgtgaac gccttatccg gcctacaacg ggcaatttta cggcccccgt
     1921 taatctcaaa ctataccatt agcactggaa gttggcacgt acaggtgggc aaaaaaacga
     1981 aaactggtca accaaatatc agtttgattg accagtaagt ctgtagggta atcagttagt
     2041 tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca gctgctgata tgccgccgtc
     2101 accgcgccaa caaagtcggc attctgcggc agatcatcgg caaaaatacc gctcaggccc
     2161 agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt taatcttctg gaactcggcc
     2221 agcatcgggt cgaccacgtc aatggcatta ccctgctcat ccacgccctg ggtgtaaccc
     2281 atccagccag ccacgcccag cgccaggtga cgccagctgc cgccgttttg caggtgcaga
     2341 cgcaccgggt ccagtagacg ctgcggtaac ttctggctgc cgtccatcgc aatctgccag
     2401 gtacggtgac gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc ataggcgttc
     2461 aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat cagggcaaag
     2521 gccgctttgc gataatccgg gttagtcatg gtgtcggcaa tggtttcata gccgccgagg
     2581 taaccgaggt acgccaggaa agagtggctg ccgttcagca tacgcagctt catcatttcg
     2641 aacggcacaa cgtctgcaac gaactgtgtg cccactttat cccagtccgg gcgaccatta
     2701 acgaagttat cttcaatcac ccactgacgg aacggttcgc aggcaatggc gcacgggtcg
     2761 taaacaccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg
     2821 cggtcaacca tggtgcacgg gaaggtgacg ttctcttcaa tccatgccgc tagctgcggg
     2881 tcacgcgcct gagccagccc cagtaccgcg accttcgcca catggccgtt ttcacgcacg
     2941 ttatcgcagg acatcaccgt aaatgctttc agcccttttt cacgacgcag acgcaaggct
     3001 tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt tttccagatc gtgcttgatc
     3061 agcgggttat tgagatccag ttgaccgctt gccgcatcag cgcagtagcc tttttccgtg
     3121 accgttagag agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat accttcgcag
     3181 ccgtcgattt ccggatgcag cgcttctttc atcgaaccga taattttcag ctcggtgctt
     3241 tctgcgcctt tttccgctac ggtgtacagc agttgctgtt ttttcaggtt ttcgatcagc
     3301 atgcggtcgt tgcctggcat caggttaact tcgcagatgc cccagtcgct gtcggtgctt
     3361 tccagcagat gatgggtata cagcgcctgg tgcgcgcggt gaaacgcccc gcaaccgaga
     3421 tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac cggcagattg
     3481 ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcagagagt gacggcaagg
     3541 aagaaggaac cggcacggcg cgtagccatg ccggtgggta ttaacggcta aagaaagcgc
     3601 gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga ccaattgcgg
     3661 agtaaccagg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg tggtccggac
     3721 gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct tcaacaatcg
     3781 ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt
     3841 tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact
     3901 gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg gtgcacatgg
     3961 tgaaaccgtt cgccatgctg tttacggtat caaccatcca ctgcatatct tcaatggtgg
     4021 aaacaatgcg cggcaagccg aggatcgggc gcggcggatc gtccgggtga accgccatac
     4081 gcacgccaac ttcttcagca actggaataa tcgctttcag gaagacggca aagttttcgc
     4141 gcagtttggc tttgtcgata tctttgtaca gctccaggtg cttacggaac tggtcgaggg
     4201 tatacccttc ttccgcgccc ggcagaccgg caatgatgtt acgcgtcaga cgcgctttgt
     4261 cttcgtcgct catagtggcg aagcgttcag ctgcctgagc aatttcttct tcggtgtaat
     4321 ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt
     4381 cgaagcgcag agctttggag ccgtctggca gcacgtattc gaggtcagta cgggtccagt
     4441 cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc acactgcgcc aggttgcgca
     4501 gagtctgctg atagttagcg atccactgct catagttgcc agtgtgggtt ttgatatctt
     4561 cgtgaattgg tacgctttct acgacagacc acaccaggcc tgcgtcttca acgatcgcct
     4621 tgcgtttgag gatctcttct acggaccata cttcgccgtt cgggatatgg tgcagcgcgg
     4681 taaccacgcc agttgcgccc gcctgacgga catcagctaa agaaaccgga tcgtttgggc
     4741 cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg ttccacagca
     4801 aagagacgcg gaaccttgga gttgtttagg tatccggatg aacaaccaga ccgcagccgt
     4861 ggcgttgcat cggatgagat ggcgtataag ttctaccttt tggtaagaca tgtcaaccag
     4921 aattctgaaa ttggttaacc gcatcgcaag aatttcactc tcatggttgt tgctgcaaat
     4981 tgcagcaaag ggtaatcgat ggttattgat cagaggatgt gacattcatc gcaacaatgg
     5041 ttgaccaatt tacataacat gtcgcccaaa ataacaacgg ttcaaccaag taaggtaaaa
     5101 cgttatgcat gtgcttaaca ttctctgggt ggtattcggc attggcctga tgctgatact
     5161 gaatttgaag ttcaaaatca attcaatggt ggctttgttg gtggcggcgc tgtccgtcgg
     5221 gatgctggca ggcatggatt tgatgtcgct gctgcacacc atgaaagcgg gcttcggcaa
     5281 cacgctggga gaactggcta tcatcgtggt gttcggtgcg gtcatcggta aattgatggt
     5341 cgactccggc gcggctcacc agatagcaca tacgctgctg gcgcgtctcg gtctgcgcta
     5401 tgtacagctg tcggtgatta tcatcggcct tattttcggt ctggcgatgt tctatgaagt
     5461 ggcctttatc atgttagcgc cgctggttat tgttattgcc gccgaagcta aaattccgtt
     5521 cctgaaactg gcgatcccgg cagtagcagc tgccactacc gcacattcac tgttcccacc
     5581 gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg gatatgggga tggtttatat
     5641 ctatggcgta ctggtgacga tcccaagtgt aatctgcgca ggtctgatcc tgccgaagtt
     5701 cctcggcaat cttgagcgcc caacgccatc attcctgaaa gcagatcaac cggtagatat
     5761 gaataatctg ccctctttcg gcgtttcgat tctggtgccg ctgatcccgg cgatcattat
     5821 gatctctacc accatcgcca atatctggct ggtaaaagat acccctgcct gggaagtggt
     5881 taactttatt ggttcctcgc cgattgcgat gtttattgcg atggtggttg cattcgtact
     5941 ctttggcacc gcgcgtggtc atgacatgca gtgggtgatg aacgcttttg aaagcgcggt
     6001 gaagagtatt gcaatggtga ttctgatcat cggtgcgggt ggcgtgctga agcagaccat
     6061 catcgacacc ggcattggcg acaccatcgg catgttgatg tcccacggca atatctcgcc
     6121 ctacattatg gcatggctga tcactgtgct aattcgtctg gcgacgggtc agggtgtcgt
     6181 ttcggcgatg accgccgccg ggattatcag tgctgcaatc cttgatccag caaccggtca
     6241 gctggttggc gtgaatccgg cgctgctggt actggcgacg gctgcgggtt ccaacaccct
     6301 cacccacatt aatgatgcat ctttctggtt gttcaaaggt tactttgacc tgtcagtaaa
     6361 agacacgttg aaaacctggg gtctgctgga gctggtcaac tccgtggttg ggctgattat
     6421 tgtgctgatt attagcatgg tagcgtaaaa gaacaccaga gcctgccaat ggcaggctca
     6481 gactgatgac taaagtctaa aaatgtgccc ggacagtccc ctcgcccctc cggggagagg
     6541 gttagggtga ggggaacaga ccagtaaagt gcgctttgtt tatgccagat gcgacgctga
     6601 cgcgtcttat ctggcctaca aagggctaac gtgcaggttt tgtaggtctg atagcgtagc
     6661 gcctcaggta ccagcattag caatgcgctg taattttttt attgataaac aaaagtcacg
     6721 ccaataatcg attgcacatt ccctgcagtc acctgccctc cggtacgtgc gtaatttgcc
     6781 gttaatccca gacttaccgc cgaagttcct actgctccta acgataccgt gttattcgct
     6841 ggaataatcg taccgttgcg cgtcaactgt acgccgacgc cctgcgctgg tgaaaacgac
     6901 gcggtattgg tgaaaatcga gttgcccgca tctgcggttg tgccggagag gtaatacccc
     6961 aggttttggc ttttcgcaca ataaacggta agaggaattg gcactgaacc agggtagtcc
     7021 ggcagagtaa cggtgacatc acgagcagaa acatcacaac cgccagtggg caccaccaca
     7081 tcattattgg cgtaaatatt ccacacaaac tgaaaatcat cgctgttata gttgttggtc
     7141 tgtcgcaaaa taagcacggc aattaatgaa ccagctttaa tcgccacccc gcccgcactg
     7201 ctcacaggcg tcaaataaag cgccaccggc cacggcttat ccgttctcga attataaaca
     7261 acccgcggcg tttcgctggt ggtcggaaat ggatagctac tgccactata ttttacggtc
     7321 ccggaaaaat tagataacac gccgccgtaa gccgagcctc gttgcagtgt gacatagtct
     7381 gtaatggttt ccggataatc gttatggcaa aagatttgcg tcgaaagatc tacgaccagg
     7441 ttttgcccca cattcacgac aggcgcaagg tttacataaa cattagcgct gccaccgcca
     7501 atagggatag cggtaccatt ggcggtttta caggcgaatg accaggcatt taccgaccag
     7561 cccatcagca gtacagcaaa cagggtaata gctcgtttca ttacaattat ctcttcgggt
     7621 tcagctgtag gtataggtga tgctaatcac tgcctgaatg gttccctgag tggctccgcc
     7681 atttaccgtc aatgctctga cctgtaacgg gaagtgcgct gattgtgagg aatcatccac
     7741 ctgaactgtt ttggttgcgc cagtattcaa tgtgttgcca ctgtcatcct gtagctctaa
     7801 ctggatgttt tgcgcggtcc cctggttttt ataatatccg gtgctgtcgg ctgccccgct
     7861 gaagctggca gtgaccctcg acgttcccac cggacaatta gtcaactcaa gcgcaacatc
     7921 atgccaggcc gatgccgccc cggcagacat aagactgaaa gaataaagat cgccgagatc
     7981 taccgtggca ttggtggtgg aaaccgtaca cggtttggcg acgaccttac cgttcaccgt
     8041 gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc gccaacattg ccgccaatac
     8101 atacccacgt ttgcaccatt tcatgagcat ctccagttac tgatattcaa gagtgaaggt
     8161 agccgtagca ttgatatgcc ccgcagtgac aggcacctgt gtcgccatta gccgggcgta
     8221 aaaattcagc gtatttggtt tacccggcgt cagggtcgtc cacgaaatcg cagacgatgg
     8281 agcattaagg ggtatttgat tttgctgctc attcagaagc tgtattccca gtcccgcagc
     8341 cgctgacacc gtattttcaa gtgcaagcag gttggcattg tggctatctg caacgccggt
     8401 aaacccaact tttacggcag aaacggcatt accacagggt gacagcaaaa tacgaaatgg
     8461 aacgacagga gtcgtcgcgc caatgttgtt aaattgcttc gccgcgtttt ccatcagatc
     8521 aacagtaaaa ttggttgatt cagcggccac actacagccg ttatctctga catagccgcg
     8581 gatagtaatc gtgctatccg cagccaaaac gcgacttacc gccagccaca aaaaagcgca
     8641 cagaagataa aaaggtttgt ttctcatcac gcccccttaa cgacattcag ctgatagctg
     8701 ggttaataac tgctgctgac tctctggtgg cagttgataa ttggcgacac agtgagcatt
     8761 ttcctcttct ccccatttca cctgaacttt tcccgctaaa ggcattccgc tgaggtaaac
     8821 ctgaccatta tccgcaacaa tgccgctact ctggctactc tctgatgtca ccatcgcccc
     8881 aaacggcagc ggcttattat tgtgagttag cgtcatgagc agttttatcc caacgcgcgc
     8941 tttaaactct gctcgcacga tcgccccacg agtgggaaca acgttagcga ccgcgttatc
     9001 taaatcgacg ttatcagcca aggtattggt atccagcgcc actctatttt cccgatattc
     9061 agtggcataa ggcagcacgg cataaccacg ccagtcggta cgcacccccg tctggttttc
     9121 gacttttgca tcttttgcgc caggcgcttt aacaagcacc accgtatcgt ttaacggctg
     9181 ccccagcgtt acgccattgg catgagccaa taccccaccg ctgactccgt aatagagctg
     9241 cttaatatca tcgctatggc tgtaaccgat attggcattg ccgtaaccac cgcgataatt
     9301 cagcgtggcg tagcctgtac ttccgctatt accatcgcct cccccggcat agccggtttg
     9361 cacgctataa ctgaggttgt tgtcttccag caacgtaccg tatacaccag ccagattggt
     9421 catccgaccg ttgagatcgt gtgacatgct gtagctggca ctggcatgtc gccactgaga
     9481 tttactgtca gaacgcagcc agtggctgaa aggaatattg acgttaagcg ctaacatctg
     9541 atcccgtcct ttttgccagg cgtttttcgt caggctatag ctgagcgtcc agttgatatc
     9601 ttcgaacgca gtatttaatc cagcctggaa ttgctcatcg acattactcg ttccccaata
     9661 agtttgatgg ctaccactca aatacagtgt tgatgtgcgc ccgagttgct gagtaacggt
     9721 gagctgtaat ttcccgcgtt tgttataagc gaggttgtaa tagtcggtga atttcggctt
     9781 aacctgaata actccatcct gtgtttcgat gttgtagcca ttcattcgac tgtatgttgt
     9841 atcagcgaaa ttaaaatatc cgctggtcga ataacggtaa cccactaact gaatattcgt
     9901 gcctgattca ttgagcgatt tgttatagag aaaacgcacc gattgtccgt catgctgact
     9961 gtcatcggga agtgtggaat tagcctgcgt catatccaca gacagagcgc ccagtgcctc
    10021 catgtttttc ccgataccga aattaaaagc acgataacga tccgccagtt gcgttccacc
    10081 atatattgtc cagccagccg gaaggccgtg gagtaatgta ctctggaaaa agcggggttt
    10141 ttcctgctgc gcatttccac tacggtattc tcctgccgta atggaataac gagtatgccc
    10201 ttcacgttgc aaaagcggga ctgacgaata gggtacggta aaaatctgcg tgctgccgtc
    10261 agcctctttg atcgttacct gcaagtcacc actattacct gcggcataga tatcgttgat
    10321 ggtaaaaggc cccggtggca ccgtactatt ataaatgcca tatccatttt gtttaatagt
    10381 gacctgtgca gtaccacgag caataccgta gatcaccggg gcaaatcctc tttggctatc
    10441 gggtaacata ttgtcatctg aggccaattg tgcgccgcga aagttaatac catcgaaaat
    10501 atcgccctgg gtataaccat cacccaacgt cagccgggaa cgtaaaggaa ttatgtctcg
    10561 ctcaatccag gtaatgatat gctgccattt atttttgcta cctgatgaac tgtcgctact
    10621 gttataactc caggtggtat tgtcgcgtaa acgccacgca ccaatattta acccactctg
    10681 taggtttaaa tatgcataat ggctgttacc cccaatccga ttctgtacac tatttccgct
    10741 gaaattataa ttgagcaatc cggcattaat accgggatcc cataactcag gaggaatata
    10801 accacgcgcg cgattactca taaatgcctg agggatcgtc aggttcagtc gctgctgacc
    10861 aacatccaga tgcgcagtag cgtcttggac cattgtggtt aatggcacac aggcatcatc
    10921 cgccagcaga ttcataccgg cgacagaagc cgtattcagc cccatactgg cgagttgcgc
    10981 gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc gtattaaatg tgacatcacg
    11041 cgttgccata taaccattat tcaaatagat atcgacgcga tacgtccctg gcggtaattc
    11101 ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg
    11161 cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac
    11221 aacgagtcgg acaaaaaaac cagccaaacg atgcttacga atatgcaagc attgtgtgtt
    11281 tcgctggtaa agtcttaaat tcagatatga cattattcct ccggtttaaa atcacccggc
    11341 agtcaattct ttttattcag gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat
    11401 tttgggggta agtgcgccat aatcatttat tgttcggtaa gtaatattgc ttcctgcatc
    11461 agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc aatgcatttt caagaacccg
    11521 ggttccggca ttcaactctg ttaccgtcag gtaatagggt gtcgggttaa tcagcgtcag
    11581 agaattcgcg ctacgacgaa atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa
    11641 tttagccggg cgatagtaca gtttaatgcg gctgataatt gcgagctgta gcatattctc
    11701 agtcaatttt gatttatcca ttgacggaat cgctttaacg ttcatccaga ataaactttc
    11761 ccggtcctgt ggcaattggt tatttgttgc atcaagaata cgtaaggtat tctctttttt
    11821 tcccttcatc gcaaacagag gaggcgtcac gataaaacga ccatccttta caccatcggc
    11881 attttccacc catgattgaa ttaaataggt actattttca tcattatttg tcacggcaag
    11941 ttgcacttgt ttttaccctg ccggataaat tacgcgagtc gcacctaagg ccactcctgc
    12001 ttcagcgcgt ccggcaacca tcattgccat gaacatcagg atacctgcca gcaagcagaa
    12061 tgttatttcc tgcgatttcc ttacattgac gtttttatta ctcactgtcc tgttcctgtt
    12121 atcacattat ctactgaaca attactgata ggttaaagag aaccaggcct gggcattggc
    12181 gatgccgcca gtaacccgac gcccggtagc acgatatttg gcgatgaaat gtagcgaagt
    12241 agagcctgag taaagccatt tccagtttgc tggaggacga ttaatcggta cgaggtttcc
    12301 ttcatcatca aacaacgcta caccaatatt ggaggctatc cctggcccct ctcccacgga
    12361 gagcacatcc ggatttttac catccgcgac accgtgaaac gccacaccca cacgttcact
    12421 caccaccgtg ctacattccc gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc
    12481 tgccgcatga aaccggttac tgctgatttg ccccatattg accgtcattt gtttatcacc
    12541 ggcttcaatc cggcaagttt ccgcaataat gacgccctga aattgcatat ttccgccggg
    12601 cagcgtggta ttccatttat ttccggccag agcaaacatt ggcagcaacg acgctaacag
    12661 aaataatctc ttccttttca tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt
    12721 aatgacgtcc ttgaacctgg gtaggttatt gatactgaac cttgaaggtt gcatccgcat
    12781 tagctgcacc cggggttgcc tcgccgattg cataataacg cgcctggaac ggaatggtgt
    12841 tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc accgtccagc gtcagagcat
    12901 tgcctgttct gtccaggatc tgcacgccaa cgtttgttgc actacctgca gcagaactct
    12961 gcagagccag tacatcagta cgcgtagcat caattgccgt acctaagaag gcaacagcgg
    13021 cttttgtggc aacagtggta tcgcaatcat tcagctgaat gttaaaacca acggcagagc
    13081 tggttgctcc agcctgcttc aggctagcgg tacgaacctg tcccaactga acggtttgat
    13141 caacagagcc tgcatcaact gcgcaagcgg cgttaacaac ttccccttta aagtgaatgg
    13201 tcccaccatt taccgtcgta gtatcggcca gagccgctgc ggaactgagg gacagagccg
    13261 acagaacaac gattgccaga gttttaattt tcatgctgct ttcctttaaa aaaactattt
    13321 ctaaatcgac atgggcagtc gttctgtaca ctttgttttg tcaacgagtt tgactgccaa
    13381 cactgcacag ttttccccca aaagatgaaa catttggggc cattttgact catagaggaa
    13441 agcatcgcgc acaaactttt tcagtttatt tgttggctta atattctatt attatcttta
    13501 tttatagatt tttatattgc atgaggtagt ttttggagag aagaatgagg aagatgcgtc
    13561 gagccacaga aacgttagct ttacatatag cggaggtgat gtgaaattaa tttacaagag
    13621 aaataattta catatcaaat agttagatgc tttttgtcgt tttttaatat ttttatgctt
    13681 gagaaaaaat acgtaactta tttatgatat ggacagtttg gccccaattg tcttgtgttt
    13741 atttgttttt taaatttatt atcaataagt taaatcaaac ctcttctctt tttaattttt
    13801 cgtttatgag attatttctt tcccataatc cggcaaaacg agcagcatta ctggcggtat
    13861 aacgcacagt atggcgaata tttcgattcc cgagataatc ctgaattaaa cgggtatctg
    13921 caccacgctc cgccagttca taaccgcaag catgccttaa catatgagga tgagtctgcg
    13981 ttacggttcc agcttcaata ccggcatcgc gaataatgcg ataggcctgc tggcgagaaa
    14041 gccgactccc gcggcgagaa ataaatatag cgtcggtccg gtcagcgcct ttccagttag
    14101 cacgttcctg ggtccagcgt tccacggctt cacgctcatc aaaacgtaac gggtgaacgg
    14161 tagaaaatcc gttcttcagt cggcgaatat ttattcgacc ttcattaagg tcaaggtcct
    14221 gataatgcag atcaagcagt tcactaatac gcatcccatg ccgatatgcc aacagaataa
    14281 gacaataatc tctggctccc gttgccccgt aacaaaccgc ctgcatcatg gcctgaactt
    14341 ctttaccggt aagataacga cgtttactca caatagtagt actcctaact gagatatatt
    14401 tgaatgaata cctataggaa acctcaatcg gtcaaaatta gcccaataga taaaaagata
    14461 accacacacg aagttatgtg gctatcagtc aattacatta aacatcatac aaaaataaaa
    14521 tatcactgat gtgacaacat ctattttcat tgatttgatt caatataaca atagattaca
    14581 gaggattaac ttccagttac ttaaaaaata aagaaaaaag tgtggaaaca ggagaattta
    14641 agtgatgtgt aaacttatga atgattttgt ttatttattt tgaagaacat aaaccattag
    14701 attttgtatg ggtatggtta acttaatgtt tattatgata attaagaatc acacttacaa
    14761 taacatcaat taataaatgg ttaatcattt ttgatatatc gtaagaataa tgtagttttt
    14821 aacaccatcc ctggtatctc aactatctct ataaaacagc atgacgctgt cgtcctctgg
    14881 ctctatccca gatgccgtaa aaacgccctg cattgctggc ggtataccag acagtatgac
    14941 gaatattgcg atgcccaaga taatcctaga taagtcgcgt atctattccc atattcgcca
    15001 aagcaaaacc acacgaatag cgtaacatgt gcggatgaat ctccagtgac aacccggcat
    15061 taccaccaga agtcgagata atatggtaaa actgttgccg agaaagcgga ttccccttac
    15121 gtgataaaaa tacccactcg ctctcagcat gcggataaga agtacggata ctcaaccagt
    15181 tttttaaagc ctgaacttct ttgttcaata gcgggtgcgt tgttgaaaag ccttttttta
    15241 atcgatggat atatatacac tttgctttaa gatcaatatc cgaaatcctc aatcgacaaa
    15301 tttcactcgc ccggaaacca tgaataaaac aaagcaaaat cagacaataa ttacgtgttg
    15361 catgaggccc ggtatttgct gctttaagga gtgactcgat ttcactatgg gtaaggaagt
    15421 tccttttttt gttatcagcc ttattcctca tcgttttccc ttataattac agacgcgcac
    15481 tagctgtgct gggttagcaa taatccaaca tttttatcct gattatgttt ataaaagcga
    15541 acgtttgctt aataacttac gtaagtggac cagttcttct gagcgaactt aaatggagta
    15601 gcgatagtta cttataataa ctttgccata caaacgccat gctcttgcat gctatgtacc
    15661 tttatatatt tatcaatctg atacgaatct aataataacc ctcatcatta atgcaactaa
    15721 tcttatctat atatcatgtg atatgttctg taacaagtaa aatcaacata aaaaattcag
    15781 caaaatttaa tatttgttaa actagccgtc tcaaatatga cattattagt taactgcatt
    15841 tgccacaaaa tggctatttc atggaacgca gtaatttctt agtggctatt atcatgctaa
    15901 ttcttagatc cctagcgatt attcctgcct ggttcgtcag cttaggccat gagattgtta
    15961 cattcctttt tatcctggag atatggggga acgcaggcga cgctttttca tctacatccg
    16021 ttggtaaata tagctttggt cactacgact taagccttcc tcaatcacag ctattcgccg
    16081 ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc
    16141 tctccagatc gacatcagga gtgaccagtt acaagccaca aaccaagaaa catatttcat
    16201 gatgagaatt atgctcagcc tgatggcgag taaaccctta ttttcaccac tctcactgag
    16261 ttagcttgca aagctttctt tgtttagatt ctaaataaat tatttataaa acatatagtt
    16321 aaaagaaaat ctttagtttt tgaagaccac cgcaagtgtt cgtctggctt cacatggcat
    16381 cttcttctta gaaaaagatc gacatatttt gtgacgcgaa ttgcaaatct ggttttgttg
    16441 tatggattgc gtgatttttg atctggtata acaggtataa agatgcacca cgatagtcaa
    16501 tgagactggg catctcgtaa tctatggcaa acatcacttc agttctttca catcgggtga
    16561 tgaaaatgca cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta
    16621 tgactctggg taaaatgggt tgagtaagtg atataactta cgaatattca aatcaattaa
    16681 aaatcagaag agattttata ctcaggtatt taatctggat ctctgtttat ttaaataatg
    16741 ttaaaagaga attttcacag gagaccttat acaaaaaata taaaatacag ttacccgttg
    16801 ccaaagacac tataagcctg gcaaaaaaat attacacaac ataaatgcta attgtttatg
    16861 cgggctttgt attgctttct gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa
    16921 tactttctgg cgtattatta ctgtgctttt cgtccccatt aatttctcag gctgcgacac
    16981 tggacgtacg tggtggatat cgtagtggaa gccacgccta tgagactcga ctcaaagtca
    17041 gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag taatacctgg aataccattc
    17101 atgataataa aaaggaaaat gccgcactca atgatgttca ggttgaagtt aattacgcaa
    17161 ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg
    17221 gcacacgcta cggaccctac gtaaaactgt cctgggacgc gacaaaagat cttaattttg
    17281 gcattcgcta tcgttacgac tggaaagctt accgacaaca agacttatcc ggtgatatgt
    17341 ctcgtgataa cgttcatcgt tgggatggat atgtcactta ccatattaat agtgatttca
    17401 ccttcgcatg gcaaacgacg ctatacagca aacagaatga ttatcgctat gcaaaccaca
    17461 agaaatgggc gacggaaaat gcctttgttc tacaatacca tatgacgccc gatattacgc
    17521 catacataga atatgactac cttgaccgcc agggtgttta caacggcaga gataatttat
    17581 cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta gtagacagga gacagtcaca
    17641 atgaataaaa ctataatggc gcttgctatc atgatggctt catttgccgc aaacgcgtct
    17701 gtattaccgg aaactcctgt gccatttaaa agtggtaccg gagcaattga taacgacact
    17761 gtctacattg gtttaggtag cgcaggtaca gcatggtaca agctagatac acaggccaaa
    17821 gataaaaaat ggactgcgtt agctgcattc cctggtggac cgagagagca agcaacctct
    17881 gcatttattg atggcaatct gtatgtgttt ggcggcattg gcaaaaacag cgagggattg
    17941 actcaggtat ttaatgacgt acacaaatac aaccccaaaa ccaatagctg ggttaaattg
    18001 atgtcgcacg cgccgatggg catggcgggt catgtgactt ttgtacacaa cggcaaggct
    18061 tatgttactg gcggtgttaa ccagaatatc ttcaatggct attttgaaga tctcaacgag
    18121 gctggaaaag attcaaccgc tatagataaa atcaacgccc actattttga caaaaaagca
    18181 gaagattatt tcttcaataa gtttctgttg tcttttgatc cctcaacaca gcaatggagt
    18241 tacgctggcg aatcgccctg gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat
    18301 aaaacctggc ttattaatgg cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa
    18361 cttgatttca ccggtaataa tttaaaatgg aataagcttg atcccgtctc atcaccagat
    18421 ggcgtcgctg gcggttttgc ggggataagc aatgattctc ttatatttgc cggaggggcc
    18481 ggattcaaag gttcacgaga aaattaccag aacggtaaga actatgcgca tgaaggcctg
    18541 aaaaaatcat atagcactga tattcatctt tggcataacg ggaaatggga taaatcgggt
    18601 gaattatcgc aaggtcgggc ctacggagta tcattgccct ggaataatag tttattaatt
    18661 attggcggtg aaactgcagg cggcaaagcg gtgacggatt cagttttaat ttctgtgaag
    18721 gataataaag tcacagtaca aaactaacgc ttcagggccc cggtaaaggg gccctgctat
    18781 caatttgtca tttgtattac gcgaaaatta tatgaacgca ataatatcac ccgattatta
    18841 ctatgttctt accgttgctg gtcagtctaa tgccatggcg tatggcgaag gactgccatt
    18901 accggacagg gaagatgcgc ctcattccag aattaaacaa ttagcgagat ttgcgcatac
    18961 gcatcccgga ggcccgacat gtcactttaa cgacattatt ccactgactc actgcccaca
    19021 cgatgttcag gatatgcagg gttatcacca tcctctggca acgaatcatc aaacacagta
    19081 cggcaccgtt ggccaggcac tgcatattgc acggaaatta ctgcccttca ttcctgataa
    19141 tgcagggatt cttatcgttc cgtgttgccg tggcggatcg gcttttaccg cgggcagcga
    19201 agggacatat tcagaacggc acggagccag ccatgatgct tgtcgttggg gaacggatac
    19261 tccgctgtac caggatttag tcagcagaac gcgagccgca ctggcaaaaa atccgcagaa
    19321 caaattcctc ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca gtgactacgc
    19381 gtcacaccct caacacttta atcatatggt tgaagccttt cgtagggatc taaaacaata
    19441 ccattctcag cttaataata ttactgacgc gccgtggttt tgcggcgata ccacctggta
    19501 ctggaaagaa aatttcccgc attcgtatga agctatttat ggcaattatc aaaataatgt
    19561 tttagccaat attattttcg tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc
    19621 gcctgatgaa gacccggacg atttaagcac gggatattac ggttcagcgt accggtcacc
    19681 ggagaactgg acgacggcac tgcgaagcag tcatttcagc acggcagccc gtcgggggat
    19741 tatttctgac agatttgtag aagcaatttt gcagttttgg cgcgaaaggt gagcggggag
    19801 tttattcatg ggggatgtcc ttgttcgttc tcagagtaga caacaaaaaa caaatgagat
    19861 caaggtaata tctcttcact tgcgggctcg aacagcaggc aattgccaat ataataatct
    19921 ggcacaaggg tgaaaaacgc gcaccgcata aaccgttgtt attgcttaca gattagccgg
    19981 atgccttaat agacatccgc gccttttcga ttatggctcg gaaatctacg aacatctaca
    20041 cagtttactg gaacgttttg ggccacagcg ttcgcagcac cgtcctgata tgccgttctg
    20101 gcgattataa gtcgacggat tctggcaaat agacgatgat gatatgctta atgatgttca
    20161 aagaaattag gccattatta tattattgct tgcattttct ttcattccta aaagcacccg
    20221 ccattaatcc caggcgcatt at
//
LOCUS       DAECNO010000075        20218 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.075, whole genome shotgun sequence.
ACCESSION   DAECNO010000075 DAECNO010000000
VERSION     DAECNO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.075"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="KKO08_002766"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="KKO08_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="KKO08_002767"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="KKO08_002767"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBD6318949.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="KKO08_002768"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="KKO08_002768"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBD6318950.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2455..3318
                     /gene="pdxY"
                     /locus_tag="KKO08_002769"
     CDS             2455..3318
                     /gene="pdxY"
                     /locus_tag="KKO08_002769"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBD6318951.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KKO08_002770"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="KKO08_002770"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBD6318952.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KKO08_002771"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="KKO08_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBD6318953.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KKO08_002772"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="KKO08_002772"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBD6318954.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KKO08_002773"
     CDS             complement(6821..7516)
                     /gene="rsxE"
                     /locus_tag="KKO08_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBD6318955.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KKO08_002774"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="KKO08_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBD6318956.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KKO08_002775"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="KKO08_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBD6318957.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KKO08_002776"
     CDS             complement(9203..11425)
                     /gene="rsxC"
                     /locus_tag="KKO08_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBD6318958.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KKO08_002777"
     CDS             complement(11418..11996)
                     /gene="rsxB"
                     /locus_tag="KKO08_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBD6318959.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KKO08_002778"
     CDS             complement(11996..12577)
                     /gene="rsxA"
                     /locus_tag="KKO08_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBD6318960.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12654..13094)
                     /locus_tag="KKO08_002779"
     CDS             complement(12654..13094)
                     /locus_tag="KKO08_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBD6318961.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KKO08_002780"
     CDS             complement(13180..13395)
                     /gene="ydgT"
                     /locus_tag="KKO08_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBD6318962.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KKO08_002781"
     CDS             complement(13668..13793)
                     /gene="blr"
                     /locus_tag="KKO08_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBD6318963.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            14036..15076
                     /locus_tag="KKO08_002782"
     CDS             14036..15076
                     /locus_tag="KKO08_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBD6318964.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(15112..16113)
                     /gene="add"
                     /locus_tag="KKO08_002783"
     CDS             complement(15112..16113)
                     /gene="add"
                     /locus_tag="KKO08_002783"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBD6318965.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KKO08_002784"
     CDS             complement(16217..17389)
                     /gene="malY"
                     /locus_tag="KKO08_002784"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBD6318966.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KKO08_002785"
     CDS             complement(17399..18991)
                     /gene="malX"
                     /locus_tag="KKO08_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBD6318967.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGIVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19166..20194
                     /gene="malI"
                     /locus_tag="KKO08_002786"
     CDS             19166..20194
                     /gene="malI"
                     /locus_tag="KKO08_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBD6318968.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         5139 a         5119 c         5201 g         4759 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt
     9301 ctggttccgc attagcctgt tgctgttcca gcttgcgtgc tttggcgcgg gcaatagcgg
     9361 cttcgacggc agctttgcgc ggatcaacct gttcttctgg ctccgcattg gcctgttgct
     9421 gttccagctt gcgtgctttg gcgcgggcaa tagccgcttc gacggcagct ttgcgcggat
     9481 caacctgttc ttctggctcc gcattggcct gttgctgttc cagcttgcgt gctttggcgc
     9541 gggcaatagc ggcttcgacg gcggctttgc gcggatcgac ctgttcttct ggttccgcat
     9601 tagcctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     9661 ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt tccagcttgc
     9721 gcgctttgga gcgggcgata gctgcttcaa cggcagtttt acgtggttca gcaacggttg
     9781 ctgcgtcgtt agtttgctcc agttctgcct gtttcgctct ggcttgcgct ttacgggctt
     9841 cccgttctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
     9901 gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc gcatctttat
     9961 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagtc gctttttcgc
    10021 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
    10081 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc aagggaatat
    10141 tgctcgggca aacccacgcg caagccccac attcaatgca gtcggcaatg ttatgcgtgg
    10201 tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc aaaagatcca
    10261 cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc tgtggttcgc
    10321 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
    10381 gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga tcggcagagg
    10441 ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt gcccagacgt
    10501 tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta atcggctcgc
    10561 catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt aatacgccga
    10621 tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag gttaattgtt
    10681 tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg ttagagtccg
    10741 ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg ccgataagaa
    10801 tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc tgagccgcgc
    10861 aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagca gcgttgataa
    10921 tcaacgtttc aatcttatct ccaccaccct gcaatttaac gcctgtcggg aatccggcac
    10981 cgcccagccc ggcaacacca aactgatgta tgcgctcgat taactcttcg cgactgcgag
    11041 agcgataatc gacccagccg tcgcgcggga tccagcagtc ttcaccatcg gcatcaataa
    11101 tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca atagccgtaa
    11161 cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg gtaagcggct
    11221 ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg ccaatatgct
    11281 gtttcagtgg aataacaaaa tgctgcgcca gtagtacctg gcgcaggggg gtaccgttgg
    11341 tctgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt ttattttttc
    11401 tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac gggaatggtg
    11461 ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga gatgcagtgc
    11521 gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat taccgtatgc
    11581 atgacacggg tagcgccaac gatggcgtct accggacaag cctgaataca tttagtgcag
    11641 ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag ctcttgcgct
    11701 tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag catcacagct
    11761 tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc ttccgcgtag
    11821 gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat ttcgtcaatt
    11881 ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc cagaatggcg
    11941 ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc attcattaca
    12001 acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg gtaattaacg
    12061 caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt tcgcggatgg
    12121 cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg tacagcgccg
    12181 actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc actgcacagt
    12241 tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg ctggttttgc
    12301 gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat gccagggtgc
    12361 gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag gcgcaaatag
    12421 acgtcagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt tccagttttt
    12481 tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag ttattgacca
    12541 gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa ataaaaaagc
    12601 cgcctattat ccgttaatcc agagcaggcg gcaacaggtt aactgtaagg ttattacgga
    12661 ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat caatgcggtt
    12721 gccaacaaag taaacagcaa ctggcgaacg gctatgccaa cttccacggg tgaaaaagcg
    12781 aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg tttaggaaca
    12841 caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca catggcgata
    12901 gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc gccgagcgtt
    12961 tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag cgtcgtactc
    13021 aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat acgttgtggc
    13081 gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct aattttaggg
    13141 gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat agtgccagac
    13201 ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct ctgcgcgacg
    13261 gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg tgtaattaag
    13321 atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt ttaataagta
    13381 gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta ggtattatta
    13441 gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata agaatgcgct
    13501 gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct ttactatccc
    13561 tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat ccgacaagtg
    13621 aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta cttgtgttgt
    13681 accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac gccaagaaga
    13741 atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt cattacagcc
    13801 cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag caataatagc
    13861 tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg gaaaattcgc
    13921 tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt aaactgttga
    13981 gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca atgacatgag
    14041 cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct tccatgcgcc
    14101 cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg atgaaacaaa
    14161 agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc tgtttaacga
    14221 tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc cgttagccaa
    14281 agcggcgctt gaggcgggta aatatgtggt cgttgataaa ccctttaccg tgacactgtc
    14341 acaagcgcga gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt ctgtattcca
    14401 taaccgtcgt tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg aaggcgtgct
    14461 gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg tgcgcgatcg
    14521 ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac cgcatcttct
    14581 tgatcaggcc attacgctat ttggtttacc ggtcagcatg acggtagatt tggcacagtt
    14641 acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc cacagcggcg
    14701 agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata tcgtgcatgg
    14761 atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc tgaaaaatgg
    14821 cgagcgtctg ccgcaggaag actggggcta cgatatgcgt gatggcgtac ttacccgcgt
    14881 ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt atccggctta
    14941 ctatgcggcg attcgtgatg cgttaaatgg cgatggtgaa aatccggttc cggcaagcca
    15001 ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac atcgcgcgac
    15061 gttgtgcctt gcgtgaaaaa tggatggtgc aatttgcacc atccattttt gttacttcgc
    15121 ggcgactttt tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca tttccagacc
    15181 attaatctgt gcctggcgga tttgctcacg ggataaccca gcagctggcg cggcaacggt
    15241 atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa tgctggcacg
    15301 aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta cggtgctggt
    15361 ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga gaaaatccat
    15421 cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac ccagttcacg
    15481 aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg taatatgcca
    15541 gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga atccaagttc
    15601 atcaccagct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg cctccagctc
    15661 ttgctgacag gcggcttcgc cgaaggttcg gctcataatg ccgataagct tcgcctgcac
    15721 accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa cacccgctac
    15781 aggcagctta tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga catagcgcag
    15841 gccgttacgg gctgcatctt caatgttttc aaatgccacg cggcggcagg catcaagaga
    15901 ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt
    15961 ggcaatgacc tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa gcgagatatt
    16021 atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg
    16081 gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct ggatataaaa
    16141 gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa cgatttttta
    16201 cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt tattaatcca gccacacctt
    16261 tttccagttt cgaacgtggg cagccggcat tgagacggac aaaaccacga ccttcttcac
    16321 cataggtata ccccggcatg atcgcgactt tttcttgctc gataagcgct ttttgcaacg
    16381 cgttgtcgtc aatattcaac ggacgtagat caagccaggc cagataagtg gattgcggga
    16441 tctgccagtt gagttcagga aacgcggcgt tcattttatc tgagatatac gtcaggttat
    16501 ctttcagata gacgcgtaag gcatccagcc acggtgcacc ttgctgatag gcggcgatat
    16561 gggcagttaa cgccagtacc gaaggggaag aaagcccatc acgtcctttc agtgccgata
    16621 aataggcatc acggctgctg ctattttcta taatcccgta agcaccggtc agcgcgggaa
    16681 tattgaaact tttcgatccc gatgtcagca acgcccagtc tccccgcgcc acattactcc
    16741 agggaatatg cggctgctcg ccccagacca tatccatatg gatttcatcg gaaataaccc
    16801 gcacatcatg acgctcgcac aggtcagcca tgatctccag ctcatcgcac gtccacactt
    16861 tcccggtagg attctgtggg ttacacagga gcataatttt acattctggt ttcgccaaaa
    16921 cggcttccag cttgcccata tcgcaaaacc agccgtcagc ctgcttctct aaagcaacgg
    16981 gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag gcgggcgtgt
    17041 ggatcaccac gccttcacct gtttcagacc actgacgaat cagttctgaa accatataga
    17101 tgacagaagg gccatacacc accgtctgcg tatcaattgc ggtgtaatgc tgggtggaaa
    17161 accagtgggc aatagccgcg agaaactcat cgtttttcca gcggctgtag ccaaatacgc
    17221 cgtgcatcag gcgctgattc agcgcctcga taatgcaggg ggcagtggca aaatccatgt
    17281 ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca taatcccact
    17341 gtgtacacca tgtgccatga cgatccacga cctttgaaaa atcgaacata tcttatcctt
    17401 atgcctggac agtatgcatc agaccggcca tttcatcttt tactgactga acttgtggcc
    17461 cgataacaac ctgcaggtta tgttgattaa gttgtactat gccaattgcc cgattgtcct
    17521 tcagtgcctg cacattaaca agcgacatat cttccacaga caaacgcaga cgggtaatgc
    17581 agttatcgag gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt gcaggaacgt
    17641 tgtaacctga tttacccggc gcaccggcaa cggctttttc gattgagctg gcaacttcgc
    17701 tatcgcgccc cggggttttc agattgaagc gggtgatagc gaaacggaag atgacgtagt
    17761 aaacgacaaa ccagattgcc gccaccactg gcaccatgta ccacttggtt gacagaccat
    17821 gcaaaatacc gaacaccacg aagtcgatga tattgccgtc ggtattaccg atggtgacgc
    17881 cgagcacaga catgacggtg aagccgaggc cggttaacag cgcgtggatg acatacagaa
    17941 ctggcgctac gaacaggaac aggaattcca gcggttcggt agtgccgcca acgacgcagg
    18001 cgatcaggcc agaaatcagc agacctttaa ttttatggcg attttccggg cgcgcgcagt
    18061 gatacatagc taacgctgca cctggtagac cgccgagaaa cgcaggcatt ttaccttgcg
    18121 aaaggaaacg cgtgacgctt tcagaaaaac cgtgagtggt cgggcaactc aattgcgcct
    18181 ggaagatggt cagtgcgccg ctgacggttt gaccgcagac ctcctgcgtg ccgcctgcgt
    18241 cggtaaagcg aattaatgcc accagaatgt gatgcagacc aaacggcaac agcagacgtt
    18301 caccggtacc aaacagcatc ggtccgaaat cacccgcgct gtttatcata tggcccaagc
    18361 cgctaatacc catggcgaaa atcggccaga ctaatggaat caccaggccg acaaggccca
    18421 tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc agcgcatccg
    18481 gcaggcggat attatggaaa cgctcatgca gcatccagac gataataccg gcgatcaccg
    18541 caccgaggat cccggtatcg atcgactgga tcccaagaat gctctggatg ttattggctt
    18601 tcagaacggc ggcatctgtg gttggcagaa tgcctttatt ggtcaaccag aagtttaccg
    18661 cgaggttcat taccgcataa ccgacgaagc cagcgaatgc cgctacgcct ttattttcgc
    18721 gtgccaggcc cagcgggatg gcgatacaga acatgacagg caggaaacta aaagcaaacg
    18781 agccaatctt actcatccag gtaaagatag cctgcaacac ggggttgccc aggaccggga
    18841 tcagggttat gacatcatgg ctgctaagag aactaccaat gccgagcata atgccgcaga
    18901 acgacaataa tgccacgggt aacatgaagg ttttgcctaa ctgctggaag aactcccaca
    18961 gcgtgacttt cggtgctgtt ttcgccgtca taaaacgact cctcgtagag aacaatctgg
    19021 attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag tcataaatca
    19081 cattacgcaa cgataatagc gggtataaga taaataaaag gtaaaacgtt ttatctgtca
    19141 cataatcagg gagtaggtca tctgcatggc taccgccaaa aaaataacca ttcatgatgt
    19201 tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg gtgcttaatg gcaaagggcg
    19261 aatctctacc gccacaggag aacgcgttaa cgccgccatt gaagagctgg gatttgtgcg
    19321 caatcgacag gcgtcggcgc tgcgcggcgg gcaaagcggc gtcattggtt tgatcgtccg
    19381 tgatttatct gcgccgtttt acgccgaatt gacggccgga ttgacggaag ctctggaagc
    19441 acagggacgg atggtttttt tgcttcacgg cggtaaagac ggcgagcagc tggcacagcg
    19501 gttttcactg ttactgaatc agggtgtcga tggtgtggta attgccgggg ctgcaggaag
    19561 cagcgatgac ctgcggcgga tggcagaaga aaaagctatc ccggtgattt tcgcttcccg
    19621 tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg ctgcacagtt
    19681 gttgacggag catctcattc gcaatgggca tcagcggatc gcctggctgg gagggcaaag
    19741 ttcctcatta acccgtgcag aacgggtggg gggctattgt gcaactctac taaaatttgg
    19801 cctgccgttt cacagcgatt gggtgttgga gtgcacttcc agccagaagc aagccgcgga
    19861 agctatcacg gcgcttttac gtcataaccc gaccatcagt gccgtggttt gctataacga
    19921 aactattgcg atgggggcat ggtttggttt gctgaaagca gggcggcaaa gcggggaaag
    19981 cggagtcgat cgttactttg agcaacaggt ttcgctggcg gcatttaccg atgcgacacc
    20041 aaccacactt gatgatatac ctgttacctg ggccagcacg ccagcgcggg aacttggtat
    20101 cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa gagacgcatt cacgcaatct
    20161 tattattccc gcccggctca ttgcggcgaa ataatcctct ctttatctgc tatacttg
//
LOCUS       DAECNO010000076        20074 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.076, whole genome shotgun sequence.
ACCESSION   DAECNO010000076 DAECNO010000000
VERSION     DAECNO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.076"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            374..577
                     /locus_tag="KKO08_002787"
                     /pseudo
     CDS             374..577
                     /locus_tag="KKO08_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            596..5260
                     /locus_tag="KKO08_002788"
                     /pseudo
     CDS             596..5260
                     /locus_tag="KKO08_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            5263..5643
                     /locus_tag="KKO08_002789"
     CDS             5263..5643
                     /locus_tag="KKO08_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318969.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(5698..5985)
                     /locus_tag="KKO08_002790"
     CDS             complement(5698..5985)
                     /locus_tag="KKO08_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBD6318970.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            6114..6623
                     /locus_tag="KKO08_002791"
     CDS             6114..6623
                     /locus_tag="KKO08_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBD6318971.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            6620..8038
                     /gene="phrB"
                     /locus_tag="KKO08_002792"
     CDS             6620..8038
                     /gene="phrB"
                     /locus_tag="KKO08_002792"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBD6318972.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(8188..9669)
                     /gene="dtpD"
                     /locus_tag="KKO08_002793"
     CDS             complement(8188..9669)
                     /gene="dtpD"
                     /locus_tag="KKO08_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBD6318973.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            9940..10683
                     /gene="ybgI"
                     /locus_tag="KKO08_002794"
     CDS             9940..10683
                     /gene="ybgI"
                     /locus_tag="KKO08_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBD6318974.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10706..11362
                     /gene="pxpB"
                     /locus_tag="KKO08_002795"
     CDS             10706..11362
                     /gene="pxpB"
                     /locus_tag="KKO08_002795"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBD6318975.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            11356..11802
                     /locus_tag="KKO08_002796"
     CDS             11356..11802
                     /locus_tag="KKO08_002796"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HBD6318976.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFCQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGD"
     gene            11833..12288
                     /locus_tag="KKO08_002797"
     CDS             11833..12288
                     /locus_tag="KKO08_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HBD6318977.1"
                     /translation="MEAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQ
                     SNRMGYRLQGQILKRITDRELLSHGLLPSVVQVPHNGQPIVLMNDAQTTGGYPRIACI
                     IEADMYHLAQIPLGQPIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            12278..13012
                     /gene="pxpA"
                     /locus_tag="KKO08_002798"
     CDS             12278..13012
                     /gene="pxpA"
                     /locus_tag="KKO08_002798"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBD6318978.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            13048..13839
                     /gene="nei"
                     /locus_tag="KKO08_002799"
     CDS             13048..13839
                     /gene="nei"
                     /locus_tag="KKO08_002799"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBD6318979.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(13836..14882)
                     /locus_tag="KKO08_002800"
     CDS             complement(13836..14882)
                     /locus_tag="KKO08_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBD6318980.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(15034..16110)
                     /locus_tag="KKO08_002801"
                     /pseudo
     CDS             complement(15034..16110)
                     /locus_tag="KKO08_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(16107..16838)
                     /locus_tag="KKO08_002802"
     CDS             complement(16107..16838)
                     /locus_tag="KKO08_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBD6318981.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            complement(16853..19312)
                     /locus_tag="KKO08_002803"
     CDS             complement(16853..19312)
                     /locus_tag="KKO08_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6318982.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(19363..19929)
                     /locus_tag="KKO08_002804"
     CDS             complement(19363..19929)
                     /locus_tag="KKO08_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBD6318983.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         4946 a         4844 c         5623 g         4661 t
ORIGIN      
        1 ttgttaataa aaagcttata gatttatagt tgataattaa aacgcatccg atatttcagc
       61 tttttctatg caagatacaa ttttataagg tgtccatttt tatatggagg cggtagcgtg
      121 tctgttacta gtggttttat ttgcatagtg aatatattgg gattgttaaa tacataacta
      181 tgtatccgct ctggtcgttg taaatattgc taattgctta atattattag tgattatggc
      241 tggaaagata aagtgtgctg taaagcggtg atgggtatta cattcatgat taaaaaaaca
      301 gaacctgtca ataaataatt cctttattaa aacggactat ttacattatg acggaatatt
      361 atctcaacga ggcggtttgg aaatacagag ccagtttctc cggggtaacg agcaggtgta
      421 ccagtgtcag gtggcatttg tattgccggg tgagcgggtc atgatggcct tcacctacgc
      481 cagaaccaca ccgctgaccc cggcagatat gacgcgctgg gctgaaatta aaaagaatct
      541 gcgcttcagg atgcggcagg aagtcaggac gaattaatca tacagggaac gggacatgga
      601 agaggcattc tgggcggcaa gacagggtga tgccctgctg catacgtcgt ttatggcaga
      661 tgtgctgggg gcggtgctgg aggtggccgc aaatgtcgtt attgatgccc tcattgtcgg
      721 cacaacctcg ttactggcga cgtcagtggg gatcacgatg gggtgcacgg ccgtcgtgct
      781 gaccggtttt gtggcgggtg tggcgatggt gtacaccggc gtgacagaaa aggtcagcga
      841 agcctgttca gccctcgcaa atatgctttt cccgcctcag attgaaggtt acatcctcac
      901 cggctctggt gacacatgga tcaacagcaa acctgccgcg cgggcagcgg cgactgcggc
      961 atcgcggcag gacatagagg cgcaggaagc gcaggcgaag gcagaacagg aagaagcgca
     1021 gcgtcaggag gatgccagaa cgttccggga tgtggccggg gaatatctgg aaatggcggg
     1081 ggccatagcc ctggcggtta acccggtgac agggcctgtg ctgatgtccc gggctctgca
     1141 cgaccagctc agtacggaag aagggcgttc tgaaatggtg gatggcgtga cacattttgt
     1201 gtcggaacta tggcagccca ctgtggcctg tgcggccccg ggatccaccc cgacccctga
     1261 cgataaaatc gactgccata aacatccttc ctcactgacg cagtttctcg cacaaaaaaa
     1321 agccgccttt ctggatgacc ccctgggaac ggcccttaac ctgctgaatc cgggcggtat
     1381 gctggaaacc gcctttcagg gagtgaatgc ggttattggc agcatcagta atctgttcaa
     1441 aggcgatgac gagccgcccg cagcggaata tatcgcggaa ggcgcgcggg atgtgcgtat
     1501 caacagccag ccggcgacac gcagcggggc acgctgtacc tgcgaggcca gggtggtgaa
     1561 tgagccgggg aacggggcgt ttgtgtcacc ggatgtgcgc attggcggtc cgttactggt
     1621 ggtcagggat atcagaagcg gcaggtcgca gataaccctg gtggcaaccg tggcgctgat
     1681 gtttcttcgc ccgggcaaaa tgctttctaa aatcgcctgt tttgctgcca gtgtcgggat
     1741 gagcatgctg acgcagaagg ccaccagtgc ccttccgcat ccggtcaatg ccgccaccgg
     1801 ggccaaatac ctggccgatg acgatgactt cgatttcagt ctgccgggcc actttccgct
     1861 ggactggcag cgcgtgtaca gcagtcgtga cgaacgtacg gaaggaatgt ttggtcaggg
     1921 ctggagtgta atgtataaag tcagcctcgt gtgcacaccg ggtagcgcag acgaaaactg
     1981 catgaccttt gtgtcgggca tggggcgacg cttggatatg gaagccgtac tgccgggggg
     2041 cgggttttac agccccggtg aagggctggc ggtaaggcgt ggtgagcagg ggcactggct
     2101 tatcagcagc gatgacgggc agtgttttct gtttgaggct gacccgcacc acccgcagcg
     2161 ccagcggctg aaaatgctgg gcgaccgtaa cagcaactgt ctgaatctgt actacgacga
     2221 ccggggccgc attgtggaaa tcagcggcga acagcagcgt ccgtgcatcc gtctgtatta
     2281 cgaactggcg gcgcatcccc ggcgggtgac acagatttat cagcatttcc ctgagactgc
     2341 acctttactg ctgcgtcgtt acagctatga cgaggccggc cacctgaacg gcgtgtacga
     2401 cagcacgggg cacctgctgc gtgagtttgc ttacgacgaa aaccactgca tgacgctgca
     2461 ccgccagccg ggcggcgaag ggtattatta ccagtggggc tggtatgaag gtccggatga
     2521 cgcggcatgg cgggtgacgg ggcaccacac ggacagcggt gaacagtacc ggctggcgtg
     2581 ggacctggcg cagcgcaggc tgtgcgtgac ggacggactg gggcgcacac gttatcacca
     2641 gtgggatgcg cagaatcagg tgacggcgta ccaggacgaa gccggacagg tgacgacatt
     2701 ccgctggagc gacgaagaac gactgttgct ggggatgacc gaccctcagg gcggcaaatg
     2761 gcgctatgtg tatgacctgc aggggcatat cacggagacg cacgacccgc tggggcgggt
     2821 ggagcaggcg cagtggcacc cggtgtggca ccagccggaa acggaggtgg acgcggcggg
     2881 aaacacctgg cgctgtgtgt acgatgaacg cggcaacctt cttgccgtga ccgacccgct
     2941 gcagcagaac acccggtatc agtatgacca ccacggctag gtggtgcaga taacggatgc
     3001 gcgcgggggg aataaatacc tccagtggaa tgaagacggg cagctcatgc ggcacacgga
     3061 ctgttccggc tcgcagacgg cgtggttcta cgacgaacgc acgaggctta tcagaatgac
     3121 ggatgcgcag agccacagca cgcgctacgg gtatgacgac agcggtcatc tgacggaggt
     3181 gatactggcg gacggccgca cggtgaatta ccagtcagac gcggcgggca ggctggtaaa
     3241 gtacaccagc ccgatgggga ggataacgcg ctggcagcgg gacgggcagg gccgggtgcg
     3301 cagccggacg gacgcgatgg ggcgcaggac ggcattcggg tacgacgctt acgggcgtct
     3361 ggtcacgctc acgaacgaga acggagaacg ctaccggttc cgccatgatg tgctggaccg
     3421 tctggcggag cagataaacc cggacggctg ccgccagtcg taccgctaca acgcgctgaa
     3481 cgcggtcacg gaagtggtgt tcaccgggga ccggggcggt gaaatccgcc accggctggc
     3541 gcgtgatgcg gcgggccggc tcacggcaaa agagacagcc gatggccgta cggaatacgt
     3601 ccatgatgcg gcagaccagc tactggaaat ccgtcgccag cggcacgaaa ctgacgcgcc
     3661 ggaagttatc cgcttcagct acgacagact ggggcggatg ctgagcgagg agacggcgca
     3721 gggcatcctg acccaccagt acgataaact gggcaaccgg actgccacca cctttccgga
     3781 cggcagaacg cagcgccatc tgtactacgg cagcggccat ctccagcaga taaatctgga
     3841 ccgggaggtc atcagcgagt tcacgcgcga ccacctgcac cgtgaagtgc tcagaagcca
     3901 ggggcgactg agcacgcggc agctttacga ccccggcggg cggctgaagc gcagggagac
     3961 atacagcggg atgcgcgggg tggtaccgga gacgttcacg gaccggcagt acagctacag
     4021 tggcgaggat gaactgctga agacgcggca cagccggcgg ggggaaacgg attacttcta
     4081 cgaccccacg gggcgcatca cggcgtgtcg ttcggaggat gaggggtatc tggcgagctg
     4141 gcagtacgac gcggcgggaa acctgctggg gaggcgcgcg ggggaaaggg ccacggcaga
     4201 aaacagcgtg gtgccgttca accggctgat gtcttaccgt ggtgtgcact accgttatga
     4261 cgaatttggc cgtgcggtgg aaaaggaggg ccggagcgga acgcagagtt accgttatga
     4321 tgcggaacac cggatggtgg aagtgacgac agcgcggggg acataccggt atgtgtacga
     4381 tgcactgggg aggcggacgg aaaaacagca catcagccct gacggtaagc cgtacaaccg
     4441 gacaaaattt ttatgggacg gaatgcggct ggcgcaggag agcaggccgg agggaacagg
     4501 cagcctgtac atctacaggg accaggggag ttatgaaccg ctggcgcggg tggataaggc
     4561 gggcaaagag ggcccgaacc ggatactgta ttttcacacg gatgtgaacg gcgcgccgga
     4621 agagatgacg gacagcgacg ggaaaatcgt gtgggagacg gggtatcagg tctggggtaa
     4681 cacgatacag gagaaggacc acggcggggt ggagcagaac ctgcgctacc agggacagta
     4741 cctcgacagg gaaacggggc tgcactacaa tctgcacagg tactatgatc cggatgtcgg
     4801 gcggttcatg gtaactgacc cgatagggtt gagaggcggt ttaaatctct acagttatgc
     4861 acctaatcct ctaaaatatg ctgatccact tggattaact ccatgtgctg ttagtaacca
     4921 aaaagcgaat cgtttattag atagtagtga aaccaaagtt actgttaggt ctagaagtga
     4981 cgctgaacaa ttgtttatgg atagatattt aggtcataat tataaaaata tgacaggtga
     5041 gtcaggcccc agcactaaga acttaatgga atatcttact ggaaataaaa caaaagcagg
     5101 ttcatatcat tgggatgata taaaagatcc ttctgtaaca aaaccttcat atagagtgtc
     5161 aggtcatgga ctaggaaatc ctgatggtga tttaccacat ttacaagttc atcaacatgg
     5221 tggaagtgtt agacatatat ttttcccgtg ggagacataa taatgaacaa tctcaataca
     5281 ttattaagtg gaaaggtttt gctcaaagaa attagtccat taatgcctgg atatagaaca
     5341 tggattgaaa tttcattgat cgatgaatta aaaccatctt atccttttag attagatgaa
     5401 tatgctatga tagggcattc accttatgcc aatgaatgta ataaagatga agctaaattt
     5461 aaactacgaa tatcatcatt tttagcttct gacatagata atgaatacga tccatcatat
     5521 gattatgttg gaaaatatga aattatcgct tcacttaatg agttaaaaac taggttaagt
     5581 actcttcatg taaacttgga gcaatttata aattcatcgg aagatgatga gtatccatta
     5641 taataagaca gaataaaaag ctaggaaggt tttaaatctt cccagctttt ttaatattta
     5701 actttcgttc agcacactaa acatcccatc ccgcagttcc tgcacctcgt cactgtcagc
     5761 gatgatggtc aggcccccct ccgaaatctt caccgtgaca ccccgtccgg tatcaaatcc
     5821 ggcctctttc agccagtcgc ccttaaggtg caggctggga tgttgtgaat aataccgggc
     5881 tataccggtt ttgcggtcct ggtggcgggc tctgacatag ccgactttat agcggcgctg
     5941 tgttttggaa atggtgactt ctggcgtaat atcgtgttca gccatgatta actaccttga
     6001 ttagttgctt gcggggagcg cgctttcgta atcttgccct gctagcggat taacttcaca
     6061 aacatcattt accaacgcct acacttactc ctgtataccg ttcaggagca gtaatgaatc
     6121 tacaacgatt tgatgacagc accctaatcc gtatcttcgc ccttcatgag ttacatcgac
     6181 tgaaagaaca tggcttaacg cgcggggcgc ttctcgatta tcacagccgc tataaactcg
     6241 tctttctggc ccattctcag cccgagtacc gcaaacttgg cccgtttgtg gctgatattc
     6301 atcagtggca aaatctggat gatttttaca accagtaccg ccaacgcgta attgttttgc
     6361 tttctcatcc cgccaacccg cgcgatcaca ccaacgtttt gatgcacgtt cagggttatt
     6421 ttcgcccgca tattgattcc acagaacgcc agcagctggc tgcgcttatc gacagttatc
     6481 gccgtggcga gcaaccactt cttgcgccgc tgatgcgtat caaacactat atggcgcttt
     6541 atcctgacgc ctggctttca gggcagcgtt atttcgaact ttggccgcgt gtgattaact
     6601 tgcgccattc aggagtttta tgactaccca tctggcctgg tttcgccagg atttacgtct
     6661 gcacgataat ctcgcactgg ctgccgcctg ccgcaattcg tctgcacgcg tgctggcgct
     6721 gtatatcgct acaccacgcc agtgggagac gcataacatg tcgccgcgtc aggctgaact
     6781 tatcaatgct caactgaatg ggctacaaat cgcgctggcg gaaaaaggta ttcctttatt
     6841 gttccgtgaa gtggatgact ttgtcgccag tgtcgaaata gttaaacagg tgtgcgcgga
     6901 aaacagcgtt actcatcttt tttataacta tcagtatgag gtgaatgagc gagcgcggga
     6961 tgtggacgtt gaaagagcgc tgcgtaacgt ggtgtgtgaa ggatttgatg acagcgtgat
     7021 cctgccgccg ggggcggtga tgaccggcaa tcacgagatg tacaaagtct ttacgccttt
     7081 taagaatgcc tggctgaaac ggctgcggga agggatgccg gagtgcgtcg ctgcaccaaa
     7141 agttcgtagt agcggatcga taaagcccgc gccatccatt acgctgaatt atcctcgtca
     7201 gtctttcgat actgcgcatt tcccggtgga agaaaaagcg gcgattgcgc aattacgcca
     7261 gttttgcgag aacggtgctg gagagtatga gcaacaacga gattttccgg cagtggaagg
     7321 caccagccgt ttgtccgcca gcttggcaac gggcgggtta tcgcctcgcc agtgtttgca
     7381 tcgcttgttg gcggaacagc cgcaggcgct ggacggtggg gccggtagtg tctggcttag
     7441 tgagctgatc tggcgcgagt tctaccgtca tctgatgacg tattacccct cgttgtgtaa
     7501 acatcgtcca tttattgcct ggacggatcg tgtgcagtgg caaagcaatc ccgcacattt
     7561 acaggcctgg cagaaaggca aaacgggata cccgattatt gatgccgcta tgcgtcagct
     7621 taacagcact ggctggatgc ataaccggct acggatgatt acagccagtt ttctggtgaa
     7681 agatttgttg atcgactggc gcgaaggcga gcgatatttc atgtcgcagc tgattgatgg
     7741 tgatttggca gctaataacg gtggctggca gtgggcggct tctacgggta ctgatgctgc
     7801 gccgtatttt cgtattttca atccgataac tcagggcgag aaatttgacc gtgagggcga
     7861 gtttattcgt cgatggttac cggagctgcg cgatgtacca gggaaagcgg tgcatgagcc
     7921 gtggaagtgg gcgcagaaag caggtgtgaa gctggattat ccgcaaccga tagtcgagca
     7981 caaagaggcg agagtacaaa cgttggcagc gtatgaggcg gcgcggaagg ggaaataaca
     8041 gacgcgtcag gcaatcgagc ccagatgccg gatgcggcgt gaacgcctaa tccagcctac
     8101 aaatttgtgc aaattcaata aattgcagga aatacgtagg cctgataaga cgcgtcaagc
     8161 gtcgcatcag gcatcggtgc tggcctatta tgactccagc gccagcgcgc ggtttctgaa
     8221 tttcagcgcc tgatacagcc aaatcatcag taccagtcct acacacgcca gtgcgcccca
     8281 ggtaatttga tcaaatactt caatatatgc attgatggag tagttgatcg ccccggaagc
     8341 atcaaacgaa gcctgcgatg tctgatcggc aatcacgccc gccagatagt tcgcgatcgc
     8401 gccagaaagc agcatgtaga tgccggttaa tacgccggtc acgcccggaa tttcaatgcg
     8461 cgtaatttgc gacatggcaa ccgggtcgat aaacagttcc gcaaagccca tcaccgcgag
     8521 gcctaacacc atcagtggca gagaagagtg accatacatt gcggaccagc gggcgcttaa
     8581 ggtcagaatg cagaatccgg cgctcatcag gccaaggcca agagcaaatt ttccccagat
     8641 gcgcacggta cgattacctg cgacgctttc tttaaccacc cacgccagga acacaccgca
     8701 cagcataact gcgaaggcat taatcgactg gaacatcgcg gtcggaacgg tataaccaaa
     8761 catatcgcgg ttaacgaagc ggtcgatata aaggctaatc gagctaccgc cctgttgtgc
     8821 gaaggcccag aacaacatac tgaagaaggt gagcgtcaca atcagcccca gctccttccg
     8881 ctgtttctgg ttttctgctt tgcgataaat ttttgccagt acacccagac caataatagt
     8941 cgcgacaatt aaggcgtata ccgaccactc tttccagaac agcacggtaa tcagtgcagg
     9001 cgttgccacc agcagaacca gcagccatcc ccagttcggc aggagaaagt ttgtcgcacg
     9061 cagtactttt ttgttaacgc cgcgggtatg agtgaaatga cgattgccac ataagaaaat
     9121 gaccagaccc gcgatcatgc caaccgccgc caggccaaag cccatcgccc agctgtactc
     9181 ttcctgggcg taaccacagg cgataggtgc gataatagat cccacgttac ccgccgcata
     9241 catcagcgag aaaccgccat cacgacgcgg atcggttggc tcatacagct cgccgagcag
     9301 acagcttacg ttagatttaa acaggccata gccgcagacg ataatcgcca gggacagata
     9361 gaggaatgac ggatggatct cactggcacc cagcaccaca tgaccgatcg ccatcaacaa
     9421 cgcccccagc atcaccgcca tgcgattgcc gagaacttta tccgccaaaa agccaccgag
     9481 gattggcgtg acatacacca gcgaacagta ggcgctaaat aactcgtagg cgtgagtatc
     9541 gttgtatttt agttgattgg tgagatagag aatcagcagg gcacgcatgc cgtaaaagct
     9601 aaaatattcc cagatttgca gcgcaacgac atagtaaata gcgcgcggtt gtgatgcgtg
     9661 tttattcata ataattccac aattgacggc aacgtcacgt aatagttgcg cggcgtgctt
     9721 gtgtttcctt aaagttaaaa ctttgataca gatctgatta tttatgcaat atgctgtctg
     9781 attgcataaa tatacattag ctgaagcgtg atgatataaa caatttgcgt cttttggcgt
     9841 cgggtttgtt tcttaatatg tcaaaagatt ggctatcgac gaacaggcga tgtaacgcta
     9901 tgttaacggt gtagctatca gaatacggaa ggcgcgaaga tgaaaaacac cgaactggaa
     9961 caactgatta acgaaaaact gaacagcgcg gcgattagtg attacgcgcc gaatggtttg
    10021 caggtggaag gcaaagagac ggtgcaaaaa attgttaccg gtgtcaccgc cagccaggct
    10081 ttgctcgatg aggcagtgcg tctgggcgca gatgcggtca ttgtgcatca tggctacttc
    10141 tggaaaggag agtctccggt cattcgcgga atgaagcgta accgtttaaa aacgttgctg
    10201 gcgaatgata tcaacctgta tggctggcat ttgccgcttg acgcacatcc tgagctgggc
    10261 aataacgcac agctggcggc gttactgggg atcacggtca tgggcgaaat tgagccgttg
    10321 gtgccgtggg gagagctgac catgcctgtg ccgggaatgg aactagcctc ctggattgaa
    10381 gcgcgtctgg gacgtaagcc gttatggtgt ggcgataccg gacctgaggt ggtccagcgc
    10441 gtcgcctggt gcacgggcgg cgggcaaagt tttatcgata gcgccgcgcg ttttggcgtg
    10501 gatgctttta ttactggcga agtttctgaa cagaccattc attcagcccg cgagcaggga
    10561 ttgcattttt atgctgcagg tcaccatgcc actgaacgcg gtggtattcg cgcattgagc
    10621 gagtggctga atgaaaatac cgatcttgat gtgaccttta ttgatattcc taatcctgca
    10681 taacgaataa tcagagggat cgaaagtgca acgagcgcgt tgttatctga taggtgaaac
    10741 ggcggtagtg ctggaactgg aaccgccggt gacgctggct agccagaaac ggatctggcg
    10801 actggcgcag cgtctggtgg atatgccgaa tgtggttgaa gccattcccg gcatgaacaa
    10861 tatcacggtg attttgcgta atcctgagtc gctggcgctg gatgccatag agcgtttgca
    10921 acgctggtgg gaggagagcg aggccctgga acctgagtct cgctttattg aaattccggt
    10981 ggtttacggt ggtgcaggcg gaccggattt ggcggtggtc gcggcgtatt gcgggttgag
    11041 cgaaaaacag gttgttgaat tgcactcctc cgtggaatac gtggtctggt ttttaggttt
    11101 tcaaccgggc ttcccgtatc tcgggagttt gccggaacaa ctacacacgc cacggcgcgc
    11161 tgaaccgcgc ttgctcgttc cggcaggttc tgtcgggatc ggcgggccgc agactggtgt
    11221 ttatccgctg gcaacgccgg gtggctggca gttgattggt catacctcac tcagcctgtt
    11281 tgatccggcg cgtgacgaac ccatcttatt acgtccggga gacagcgtgc gatttgtacc
    11341 gcagaaggag ggaatatgct gaagattatt cgtgcgggca tgtacaccac tgtgcaggat
    11401 ggcggtcgtc acggtttttg ccagtcgggt atcagccact gtggcgcact ggatatgcct
    11461 gcgctgcgca ttgctaacct gctggtgggt aatgacgcca atgcccccgc gctggagatc
    11521 acgctcggtc agttaacagt tgagttcgaa actgatgggt ggtttgccct gacgggtgca
    11581 ggttgcgaag cgcggttgga tgataacgcc gtctggaccg gctggcgatt gccgatgaaa
    11641 gcaggccagc gtttaacgct taaacgcccg cagcacggga tgcgcagtta tctggcggtc
    11701 gcgggtggta ttgatgttcc gccggtaatg gggtcatgca gcaccgatct caaagtgggg
    11761 attggcgggc tggaaggccg tttactgaag gatggtgact gactcccgat tggcaaatcg
    11821 aagcgtgatt ttatggaagc gcagggcgtt aaacagctgc tgtggggcaa ccgcattcgc
    11881 gccttgccgg ggccggaata tcatgagttc gatcgcgcct cgcaggatgc attctggcgt
    11941 tcgccctggc agcttagctc gcaaagtaac cgaatgggct atcgtttaca ggggcaaatt
    12001 ttaaaacgca tcaccgatcg cgaactgtta tctcacggtt tgttaccgag cgtggtgcag
    12061 gtgccgcata acgggcagcc gattgtgttg atgaacgacg cacagaccac cggtggttat
    12121 ccacgtattg cctgtatcat tgaggctgat atgtaccatc tggcgcaaat tccgctcggt
    12181 cagccgattc attttgtcca gtgttcactg gaagaggcac tgaaagcgcg gcaagatcag
    12241 caacgttatt ttgaacaatt agcgtggcgg ctgcacaatg aaaattgacc tgaacgccga
    12301 tctgggcgaa ggctgcgcca gcgacgcaga gctattaacg ctggtttcct ctgccaatat
    12361 tgcttgtgga tttcatgcag gcgatgcgca aaccatgcag gcttgcgtgc gtgaagcaat
    12421 aaaaaatggt gtcgcgattg gcgctcaccc gagttttccc gacagggaaa attttggtcg
    12481 cagcgccatg cagctgccgc cggaaaccgt ttacgcccag acgctgtatc aaattggcgc
    12541 gctggcaacg attgcccgtg cgcaaggcgg cgtaatgcgt catgtcaaac cgcacggcat
    12601 gttgtacaac caggcggcga aagaagcaca actggcagac gccatcgcca gagcggtata
    12661 cgcttgcgat ccggcattgg ttctcgttgg gctggcagga agcgagctga ttcgtgcagg
    12721 caagcaatat ggcctgacaa cgcgcgagga agtgtttgcc gatcgcggtt atcaggctga
    12781 cggctcgctg gtgccgcgaa gtcagccagg cgcgttgatt gaaaacgaag aacaggcgtt
    12841 ggcgcaaacg ctggagatgg tgcaacacgg cagagtcaaa agtatcaccg gcgaatgggc
    12901 aacggtcgcg gcgcaaacgg tctgcctgca tggcgacggc gagcacgctc tggcgttcgc
    12961 ccgccgatta cgagctacat ttgccaaaaa ggggattgtt gtcgcagcat aaccccgctt
    13021 aataaagaat gaaaaaagga tatcaccatg cctgaaggcc cggagatccg ccgtgcagcg
    13081 gataacctgg aggcggcgat caaaggcaaa ccactaactg atgtctggtt tgccttcccg
    13141 cagttaaaaa cttatcaatc acaacttatc ggtcaacacg ttacccatgt ggaaacgcgt
    13201 ggtaaggcgt tgttaactca ttttcccaac ggtttaacgc tctacagcca taatcagctt
    13261 tacggcgtct ggcgcgtggt tgataccggc gaagagccgc agaccacgcg agtattgcgg
    13321 gtaaaactgc aaacggctga caaaaccatt ctgctttata gcgcctcgga tattgagatg
    13381 ttgaggccgg aacaactgac cacgcatccg tttttacaac gcgttggtcc cgatgtgctg
    13441 gatccgaatc tgacgccgga ggtggtaaag gaacggttat tgtcgccgcg ctttcgtaac
    13501 cgtcagtttg caggattact gctcgatcag gcgtttctgg ctgggcttgg caattatttg
    13561 cgggtggaga tcctctggca ggttgggttg actggaaatc ataaagcgaa agatctcaat
    13621 gcggcgcaac tggatgcact cgcacacgcg ttactggaga ttcctcggtt ttcttacgct
    13681 acgcgggggc aggtggatga gaataagcat catggggcgc tgtttcgctt taaggttttt
    13741 catcgtgatg gcgaactgtg cgaacgctgt ggcggcatca ttgagaaaac cacgttgtcc
    13801 tcgcgcccgt tttactggtg tccaggctgc cagcactagg ccgaccgctt cggcgcatag
    13861 gttgaaataa accgcgcaat ggctggcccc gtcagcaaaa tactgaacag gcgcagggtt
    13921 tgcatcgcca taataagcgc catatcggca ttgctccctg cggcgatgac cgccacggta
    13981 tcaagcccgc cgggactggt ggcgaggtag gcagtcataa aatcaatatg cataaaccgg
    14041 gtcagtcccc acgccatacc tgcacaaata gccagcagag caaaaatcga cagcaggatt
    14101 tgcggtagcg ggcgtagtgc ccgcagtaag atttgtttat cgaaaccaag accaatccgc
    14161 cagccaattg ccatatacgc catcgccagc agccattccg gtagttcgat ggtgatgagc
    14221 tgaccagact ggagcactgc gcccgccagc atcgggatga gcatcgtgcc ggaaggaaga
    14281 cgcagcatgc atcccgccgt accggcaacg actgcgagca aaatcgtaag caggagatta
    14341 atgctcactg gcgggaacca gacgatttgc tggttaaccg cttcagcgtt atcacccagc
    14401 atcatgcggg tgaccagaac agcggccccc gcgacgaaca gcactcgcag atattgcata
    14461 aacgccacca ggcgaatatc tgcgccgtaa tcttgcgcca tagcgaccat tgccgctgcg
    14521 ccgcctggag aagaacccca ggcaccggta tttccaggca gtgagctata gcgcaccaat
    14581 aaccaaccca cgatggcgct ggaaagcagc gtcaccagta aaatagcaag cacgatcggc
    14641 cagttggcgg ccagcgtggt gagaatcgaa ccagtgaggt tttgcgcaat catgcagcca
    14701 agaatggcct gcgcggcgag aaaagcggag cggggaagtt gcagagtaat tccgcgcata
    14761 ctgaagatga tcccggcgat catcggccca agtaatagcg ccgccgggag atgtaccgcg
    14821 aggaaaccaa tagaaaggag aagtgacagc acacataaca ttccccactg caaaactggc
    14881 atccttcgct ccctcattat tttctctttg gtgtatcgat acagcataaa gaaagccggg
    14941 ctaggaaaaa ataacagcaa tcaaggaaaa ggggaaaatc agcaattttc tgaaagagat
    15001 gccctttccg gctgcaaagg gcatcatggc aaatcagtta tagttaattt taaaaagcac
    15061 cactgtatcg aagggcccag cctcgatggg ggtacctgca atggacgaaa gttcagctgt
    15121 ataggttttt tcgtagacca acatgtcgct ggtgaaatcg gcgtattcag catatttatt
    15181 gaaggtataa ggcgaggcgt tttcatccag caaacgtagc ttcagaccat taccaatcaa
    15241 caatgcttta tcttcttcaa ccaacgtttc ttcggtataa aaggaggaac tgactttaaa
    15301 tccgtcggtg cattggtcat tttgtgattt tgtcgtctta atactgaatg ttttcgacat
    15361 tgtgtggcga cgaatatcca gcaaattaaa tttaccaaaa tcaatcatct gtgtttccgg
    15421 tgaaatggaa aaattgacgc tgcaatccag cacgcgaatg ttttccagac cagtaatatg
    15481 atatttcagg ttttgggcag tagggtcttc gttaacgcta ccggcaccgt caaattgcac
    15541 gacgatatag tcgctaagcg tactttgata atcatgcggc ggcatttctc gtattttgac
    15601 gtataaccgc atgcgagcga gaaaggttcg cgacatgtga atattagcgg gatcacccga
    15661 gcaaattttg ttctgccatc ccatcagggg tataaatgtc aatgttctta ctgtcgatac
    15721 actggttagt gtcgatgcgg ctttttccct ggttggcgtc ataatcaacg ccattgtaag
    15781 ttactccgag ttgataataa cgatcctgca cccccgggta ggggtttacc catgcgtaga
    15841 catgttcgct ttcaaagttg ccgttggtat tgttgtcaca atagacggga attttaatat
    15901 cgtcagattc ccagatctta ttttaatatc gtcagattcc cagatcttat cgccgagttt
    15961 ggcattgcct ggtacagcaa aaggttgaat cgcttctgat ttttctaccg agccaccgga
    16021 tgaaccaaag tagcagttca gggcaagcgc agacttcaac gacagaaata acagacagat
    16081 gacgagcaac actcccttgc cagcactcat ttcttcgcct ccacggcttg taaatggcat
    16141 tgtccgctgc agttgagcgt cgtcatgcgt aacgcgccgt aatcatccat ataaccgaga
    16201 taaaactggc tgccgctata atttccggtg tttgtgttga ccgtactaaa aggcgcaacc
    16261 atcacggttt tgaagccggg gagaacgcct ttttcatttc gtcccaggta ggcaatggtc
    16321 agataatacg cggtaggatt tttcagtgtc agttggttgc cctgctggct gacctgtaac
    16381 tgcagttcga ccttttctcc tgctttcttg cgtaatgcag ccggtcgcca gaacaccttg
    16441 atacgactct gaatggcgac ttgcagaacg gcatggtcgc tgtttttttc cggggcggga
    16501 ggaatttcac gcatattgta gaaaaatagc gtttcgcgat cgcccggtaa ttttgtggtc
    16561 gaggcttgct ttactatccg tacctgtgtt gttgctttcg gttccagacg ttgtatcggc
    16621 ggcagggcga ccagaagatc atcgcttttc tccccttttt cattttcgag ccaggaataa
    16681 gcgaggtagg ggagtttatc gctacgatta tccacccgca gactggtagc tttatcatta
    16741 gcgttaaaca caatacgagt gcgatcgggt tgtagtgcag cgtggctgca cagactggca
    16801 accaacaaca aaaggggtag tcctttcata aatgtcattt tcatttcctt aattaatgac
    16861 aaggtaaaat caggcgattt gcggtgtctt ccatatgttc aggaagatgg agcgagcaat
    16921 gctggctatc accccagacc acggtaaact tttgattttc agcgactccg cttaaccagg
    16981 catgtccttc ctcgccaacc atccccacgc taatgccgct gtcatcctgg cggatatccg
    17041 ctccgagggg aggaaactga ccgctggcgt tgcgaatgat gacgttaacg tctttaccgg
    17101 aacgggaagc cagtgatttg taacctatcg cgccttcaat ccacgtttct ttgataacgt
    17161 tttctgcaac tgttacgccg tcgggtaagt cattcatgtt caccgccacg gtggaaggct
    17221 ggtaactgga aatcaacggc accaccgcaa tgccaaaatg gttggtgtag tcgagattgc
    17281 cctgaaccgg aatatctgcc acgccatcgg tgctgaccat caggcgtggt tcattggtgg
    17341 agctgcggcg atgaaacgct gcaccatatt gggttgcggt gaaagaaccg ctccagctgc
    17401 tgctgacgga actgtaatca ttggcggcat aggtaccaga aatatcccac tcacccgctg
    17461 aactcaggtg ctgatagtta ccgctcacct gggctccatt gtcaggacgg tcggattgca
    17521 gtccggcaga catgccccag ctattacgtt catccagcgt atcgttccac gacatgcggt
    17581 gtgtggtgct gtggctactg ttttgcatgt catagccaac ccgaccaccg ttaccgaagg
    17641 ggagcgaaat cgacaggtaa atctggttgt cgcgatcttt atcttcgtaa tgggttgtat
    17701 tgaatgacgt agaaatagag atatctctcc agtcaccaat atcaacatta aaacctgccg
    17761 tgatgttggc ggtcgtggag gcgtctgcat tccaccaggt ttgatgtagc aggttggcgt
    17821 aaagattgag gtttagtggg gtaatcggtt ggcccacaga taagctgatc gtctgttttt
    17881 cgtcctgcgc atcgctgtcg ttgtatttgt gatccagata gttggcgtag ctgtgaaaat
    17941 gacgatcgga gaaacgataa gcggcgaggg aaatcgtgct gttagtggca tccacttgtt
    18001 tgctgtaatt aaaacggtag cttaagcccc gctcgtcctg ctgagtatca aaatggctac
    18061 tggcccacgt gacatcaaac gacagcgcac caagccacag catattttgc ccaataccca
    18121 ttgcggcaga atggtagtca tcaccagaaa gcagcaggcc gctgtacagc gaggtgtttg
    18181 acagcatccc ccaggaaact tcattgctaa aaaaggtttc attttcagtt tgatgtgaca
    18241 tggagggccg tggctgaccc gcggccagtt tatagcgaac ctgtccctga cgagtcagga
    18301 agggcgtcga tgctgctgaa acctggaaat tgttcacccg accatcttct tccgtcactt
    18361 tgacatccag tgtgccctga acagactgat taaggtcgtc aatgataaat gggcctggtg
    18421 ggactttttt ctggtaaatg acgcggcctg attgactgat cgtcaccgtg gcattggtct
    18481 gtgcaatacc gctaatttgt ggggcgtagc cgcgtagttc ccatggcaac attcgatcgt
    18541 cacttgccag tgccgcgccg gtataagaaa aaccgtcgaa aatattggga ctaaaatccg
    18601 tttcgccgag ggttaatttt gaacctaatt gcggtaacgg gcgaaaaaga taggtacgtg
    18661 atatttcgcc tgactgttca tggttatcat cgctatcagt atgattcaac tgataatcac
    18721 tgcgtaagcg ccatgccccc gtgttgattc cggctgttcc ataggcgtta agattagtgc
    18781 tgctgctgcc atcctgtggg cggtagctgc tggcaaacaa gttgtaatcc atcaggacac
    18841 cggcgacacc ttctttccat gtggagggtg gggtccagtt ttctgagtgc cacgccagcc
    18901 aggcttgcgg aatactaata ttcagttgct gattggcttg atcgaaattg aagagcattt
    18961 caggtcggga gctaaaatcg acgcactgat ttatcaatgg taacgactga cggacttcag
    19021 gttttaagcc aaatttatcg accagtaaat cattgatgca cggaatggtt ttatcgtcat
    19081 ttttgtgcca gttaatcttt tgctcgttac tgatttggtt attatttacc gcaacgctaa
    19141 caaaatactc accgggagca atgactcctt tttcctttaa taatgaaata tcaatgcggt
    19201 cgcgcattga tttatcgaga acattcagat tgaattcgac cgccaatgcg caaggcatcg
    19261 ccacgaccag gcaggataca aaagagagtc gataaatatt cacggtgtcc atacctgata
    19321 aatattttat gaaaggcggc gatagcgccg ccaaagtaat acttatttat aatccagcac
    19381 gtaggttgca ttcgccgtaa ctgcgcctgg cgttacaggt gtagcctgat caatttgttc
    19441 catccaggca aagaaattca gcgtttgttc gctggaggtc ggagccaggt cgatatctgg
    19501 agtagcggtg cctaaaacga tattgctttg atctttattc attaaacgta cgccgacgcc
    19561 ggtggcttca cccgtgctgg tgttgttgag aatcggatct gcgccggtgg ttttcgctga
    19621 gctatcaaag gtgactgcaa ctttagaaat cttgccaccg gaaccgttgc tggagttttc
    19681 cagatcacag ttgactaaga gcaggtcgac agcaacggca tcgctgtgat gtgactgatt
    19741 aatgtggctg gtggtcacct gaccgagttc aacctcttta tcaatatcat cctgatgtat
    19801 ttcacacggt gcttcaataa cttcaccttt aaagtgaatt tcaccggatc cttcatcagc
    19861 cgcataaaca ggtgctgtga acagcagaga tacggccagt gcggccaatg ttttttgtcc
    19921 tttaaacata actgagtcct ttaacagaaa agaaaaaagt atatcggtgc aagaatatct
    19981 tatttgattg tcgaatgttt ttagtttttc ttaaaaatat tattaatttt attaaatgta
    20041 aaagtaatta attgatggta tgaattaaat gttt
//
LOCUS       DAECNO010000077        20029 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.077, whole genome shotgun sequence.
ACCESSION   DAECNO010000077 DAECNO010000000
VERSION     DAECNO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.077"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            60..491
                     /locus_tag="KKO08_002805"
     CDS             60..491
                     /locus_tag="KKO08_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318984.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(523..1050)
                     /locus_tag="KKO08_002806"
     CDS             complement(523..1050)
                     /locus_tag="KKO08_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBD6318985.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(1043..1789)
                     /locus_tag="KKO08_002807"
     CDS             complement(1043..1789)
                     /locus_tag="KKO08_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBD6318986.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1804..3711)
                     /locus_tag="KKO08_002808"
     CDS             complement(1804..3711)
                     /locus_tag="KKO08_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HBD6318987.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
     gene            complement(4420..4812)
                     /locus_tag="KKO08_002809"
     CDS             complement(4420..4812)
                     /locus_tag="KKO08_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HBD6318988.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
     gene            complement(4809..7010)
                     /locus_tag="KKO08_002810"
     CDS             complement(4809..7010)
                     /locus_tag="KKO08_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBD6318989.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            complement(7007..7315)
                     /locus_tag="KKO08_002811"
     CDS             complement(7007..7315)
                     /locus_tag="KKO08_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318990.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            complement(7291..7626)
                     /locus_tag="KKO08_002812"
     CDS             complement(7291..7626)
                     /locus_tag="KKO08_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318991.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            complement(7653..7970)
                     /locus_tag="KKO08_002813"
     CDS             complement(7653..7970)
                     /locus_tag="KKO08_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318992.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
     gene            complement(8061..8462)
                     /locus_tag="KKO08_002814"
     CDS             complement(8061..8462)
                     /locus_tag="KKO08_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318993.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
     gene            complement(8449..9498)
                     /gene="virB11"
                     /locus_tag="KKO08_002815"
     CDS             complement(8449..9498)
                     /gene="virB11"
                     /locus_tag="KKO08_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HBD6318994.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            complement(9502..10698)
                     /locus_tag="KKO08_002816"
     CDS             complement(9502..10698)
                     /locus_tag="KKO08_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HBD6318995.1"
                     /translation="MMNEQEDIKTAAELEAEARERARAEIDGDKPAAGAVPPGQPQVV
                     QHAKRRSRRSLVLAAISLVLLVIIATGGDYLMAKLKGDGSGKSASHEPESHATSPARE
                     RNNLGRDLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTASSA
                     PKSTSSSNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIPCA
                     MMQRFVSDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLAIT
                     ELRTPEPGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAASG
                     SSPGKDRNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFSDV
                     YRLRMR"
     gene            complement(10695..11588)
                     /gene="virB9"
                     /locus_tag="KKO08_002817"
     CDS             complement(10695..11588)
                     /gene="virB9"
                     /locus_tag="KKO08_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HBD6318996.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPVKQPVTDADGKTVQ
                     KIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNLAFVVRFSYPDDTRRQNDAA
                     RLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAGSESITPDFAYDDGRFTYLG
                     FSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSLSPRFVLRYGSQVVGIENGS
                     YGKVTVANGSTVSPDVKLESK"
     gene            complement(11581..12267)
                     /locus_tag="KKO08_002818"
     CDS             complement(11581..12267)
                     /locus_tag="KKO08_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBD6318997.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            complement(12260..12400)
                     /locus_tag="KKO08_002819"
     CDS             complement(12260..12400)
                     /locus_tag="KKO08_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6318998.1"
                     /translation="MKYGITLLMLCLLAGCAQRNTQLPPVSGNPEPVNSPAIIQELTP
                     HV"
     gene            complement(12477..13484)
                     /locus_tag="KKO08_002820"
     CDS             complement(12477..13484)
                     /locus_tag="KKO08_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBD6318999.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     LAGATKASDANYLTLAVQVFLGTAGAAAVMVLTYKLAEALAGASAQGAAQALAASGVG
                     SARQSASSAAGAVGDKASQNLNSTGSATASSASTASRATGDARSRSRAAVQRMQERYQ
                     R"
     gene            complement(13502..13786)
                     /locus_tag="KKO08_002821"
     CDS             complement(13502..13786)
                     /locus_tag="KKO08_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HBD6319000.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            complement(13798..14514)
                     /locus_tag="KKO08_002822"
     CDS             complement(13798..14514)
                     /locus_tag="KKO08_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBD6319001.1"
                     /translation="MKLKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKL
                     QQWQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLL
                     TGSGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKIS
                     DTLSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLA
                     EQRRKAQAEQMRTAPIADLN"
     gene            complement(14533..17283)
                     /locus_tag="KKO08_002823"
     CDS             complement(14533..17283)
                     /locus_tag="KKO08_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HBD6319002.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLL
                     LIPAFIELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDVTEFIK
                     HMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLATLDMQL
                     NNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFYQRRLF
                     FTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYE
                     ENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYF
                     RGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDD
                     AISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDLGFIMT
                     LSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGEAIAVV
                     PTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQKYRRPE
                     SFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNF
                     LKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKD
                     ARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFY
                     LLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQSPEEM
                     CKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQMVIMKS
                     PLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDEMLRR
                     AV"
     gene            complement(17293..17592)
                     /locus_tag="KKO08_002824"
     CDS             complement(17293..17592)
                     /locus_tag="KKO08_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HBD6319003.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(17603..18265)
                     /locus_tag="KKO08_002825"
     CDS             complement(17603..18265)
                     /locus_tag="KKO08_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBD6319004.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(18268..18483)
                     /locus_tag="KKO08_002826"
     CDS             complement(18268..18483)
                     /locus_tag="KKO08_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319005.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(18476..18784)
                     /locus_tag="KKO08_002827"
     CDS             complement(18476..18784)
                     /locus_tag="KKO08_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319006.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            19098..20012
                     /locus_tag="KKO08_002828"
     CDS             19098..20012
                     /locus_tag="KKO08_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBD6319007.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
BASE COUNT         4207 a         5470 c         5702 g         4650 t
ORIGIN      
        1 gggcaggccg aaccgtcagg cgatacgaag acgaaattca cactgacgga gaaacgacca
       61 tgacgatgaa cctgcacacc ccgacaaccg cccctaaagc tgcaccggcg accagcgtat
      121 ccccgctgga gcagtcaggc tccccaaaaa cgaaatttcc tgaaatcaaa accgtcaaaa
      181 cggcggtggt taaactgatc agcgacatcg agggcaacct cgatcagcgt ctggtatatc
      241 gcgaggccgt agagggattg tgccaggagg cggccaaccg gataacccag tgcatgcatt
      301 cacgcagtac agggcagacg ctacgtgatc tccgcgagct gctgtcggtg acgctggaca
      361 ccattatcgt ggtggagttt gaaggggagc agacccgtct caactatggc ggacgttcag
      421 cagaagacgt gatcgccatg ctgaaagcgc tgcatcttga aggacgcaaa gcctggctgg
      481 aagtggagta aaaaaaaagc cccgtccgca gacggggctg attcagtaca gggcattcag
      541 cgggatgccc tcatcccaca ggcggttaaa ttctcgctgg tacgcctccg ccagctgcgg
      601 cgcatcctcg atcagcagca cgttttccgc gttgcgcgaa acggcgcttg ccgtgtagtt
      661 aaacgacccg gtctggacgt ttttgccgtc gatcaccata aacttgttgt gcatgatggc
      721 gtaacggccg ttaaggtgca ccggcacgcc ctggtttatc agatacgtca cggcggtgta
      781 gcggtcgctg ttcgcctttt cgtcggccac cacgcgcacg gccacgccgc gccggttagc
      841 accggcgagc gccgtcgcga ccggcttact ggtgaagctg taggccgcga cgtcaatgct
      901 ctgcctggcg ttgtttatgg cgccgagcac gatttcaaga gcagggcgcc cgtcagacgg
      961 cgaaaaaccc gccgtcagga cgggcgcggc ggtggcgaac gcgggcacgc agagggcggc
     1021 ggccagcacc agaagggtgg ttttacgcat agttgtccag ctccaggttt ttcagttcat
     1081 cgagggtgta aaagcggaaa acgtcgcggt gttttgcctc cagttgcaca tgctggtttt
     1141 tcacgatcac gctggcgatg ctgtcgaaaa taatcttcag ggcgcgcttt ttcgcctcgt
     1201 ccggggcgac gtaaaacgcg taccgccatt ttttatcgct gatagccagc aggtggctcg
     1261 ccatgatgga ctgatagcgc gcctttgttt tgatacgtcg ttcggtttca acggcgatca
     1321 gcctgccgtc cggcaggctg ataagcccgt cgggacggtg tttgacgctg tactgactga
     1381 ggaacgttga acggtcgccg ttaatccacc ctgtcgcgcc gttcttctcc agcgtaaggc
     1441 gcactagctg gttatcaagg tgatggtcga gtgaccagcc ggtcaggcgg tagggttcaa
     1501 agcgggcggg cattatgttg tcgtccgccg tgaggataac ggccagcccg tcggtggtaa
     1561 tgccccacag ggatattctc gacgcgcggg attcatgcac gtgcttctga atcagcccca
     1621 ttttctccac cttctccaac aaccggtaga gagggcggtc agatttaaaa tcaaaaagca
     1681 gctttatgat ttgaaaatcg ctgtaggttt cctccttgag aaaattcagc agtacgcgaa
     1741 ttttttcagt gctgcgtgca taacgttcct gatacgacga aataagcata gaaactcctt
     1801 attttataaa tccagcaacg aatgtatttc gtcatttgga gcgggctgtg atgccatcag
     1861 ccactcctcg tcaggttgca ccggcggagt gggtattggc gttgctgagg gttttttggt
     1921 ggttgttgcg ctgaccggaa taatgtcagg tgccggaact gccggagccg ttttatcaaa
     1981 atccagatcc agcaccggtt taacttctgc caccgtagcg actgcgaccg gcggcagggt
     2041 aaacagctcc agtggggctt ttttaacctt gagcggtgag attaacgacg ggaacgcgag
     2101 cgcgttctgg ctgaagatat agctgacgaa cttcggcagg ttctggagca tattgctgtc
     2161 gatgtaatag ctttccgcct gccgaagcat gcgctcgtta tcgacttttt ctgtcagcac
     2221 cgccgtggtg gtgactttcc ggctttcatc atcgactaaa atcgtgcccg acatgcgcgc
     2281 aatccattcg gcggtgtctg gatcctgaag tttatatacc agcttaaact tgcagttttc
     2341 cacaaccgcc ccaaccacgg cgtcgccgtt taaatccgcc ggacagtcgc gcaggtcggc
     2401 aattgactgg tgcgccatta atatatggac gcctttatcg cgtgccgcgc ctaacccttc
     2461 gagcgccggt ttggacaggt gatatttcac ctcgtcctta aagatggcga tggggcggat
     2521 attccccgcg acgcggtcgc gcaattccgc gagctggaac aggcgaacta aaatcatgcg
     2581 ctgcgcggta atgattttag agttacgcat cgaaccgatg atatagcagc agccgccctt
     2641 gtcgaaaatc tccttcagcg caaaaccgcc tggcgcgtta atgctgttga gcattgccag
     2701 ctcctcggtt ttaccgtgaa aggctttaat cgtctcgcca atgccctgaa cataatcgcc
     2761 gttataaata tcccgcacgg ttgcgcccgg attatccttc acgaactgcg ccgccgttct
     2821 cgccgccttg cggtcgtcga tacggtaaaa atccgccgcc tcgccttttt ccgccagact
     2881 gaaaccggcc acgaataatt cctccagatg gtcggcggtg ataccgtcga taaggttcat
     2941 ctggtattcc tgtttattca ggtcgataag cacaaagggt tttccggcgt ccgcgcacgc
     3001 ttttttatac aggtgcggtg cccactcgtc gtttttgggg tcgagaacaa aaacgccctc
     3061 gtcggcgagg atgctctgat aaagcagaat accggttacg acgcctttac cggcaccagt
     3121 ggtgccgata atgtcggcgt gctgggtctg ccattctttt aacggaatat attgcggctc
     3181 accctcgcgg ctcagcccga taaaaatccc cttattcaga tcgatgtaat ccatcggatc
     3241 gtattctgtg gtgacgggta aaatttcttt tacccggcga acgtcggttc tgcggtcgcg
     3301 ctccatgctg gtttttttca ccatgccgca tttgacctta tcaaattcag gcgagatcaa
     3361 acgccgtaaa accacgtgca gaataaaacc ggcgacggta aacagtagcg ccattgtcca
     3421 ggtaagcagc ggcggactgg cgatatcttc ccggccataa atgcgctgta aaaaccagac
     3481 ggccactggc gctatcgagc cgaaaacaaa acagatcatg gaaagcagaa ttaagatcct
     3541 ttgcagaaac gtcggctttt cctgaccgcc gtgtttcgca gaaaatataa aaggcagcat
     3601 gacgccggac agcagcgcta acgccagttg atgcgcctgc aaaaaggcga taaaggccag
     3661 gacggcattt gctatcggcg acagcaaatg attcagtttg tcgaatatca cgatattctc
     3721 cttcacttaa attcggtcag caccatcggt cagtcagaac ggtcacaggt tgtgatcgtt
     3781 ctgactgacc gattccatgc tgcaaaagaa gtggccgcta agcggccatg atcgcaggag
     3841 gtacactccc gcgatctaac ggcgtgcggc attacgcgcg ggctggacgc ccttactgca
     3901 tgtcgcatgg gggtaatgtc ctggctgaac gcccggccaa aacccccaag gtcaaaagag
     3961 gccacaccgt cgcgcgctac gcgcgaccac cccctaaaag acgcctgttt tagccctttt
     4021 cttcgccttg ttcgttcctc acaagacgaa ttaaagggaa actaggcttt ttagggggtg
     4081 gtcgcgccac gctaccggct tttggccgga gacttaggca cgacggtgtg gggggcaagc
     4141 cccccaaata aaccgacgcg tgggcgcgtc tttttgctcg ctggggcggc aaaatttatt
     4201 cggccccccc cggctttttt cggctcactt ggggtgagcg ctcaaaaagt ggaatcggcg
     4261 acgcaaatta gcaatttctt cgaaattgaa tttactcgcc tcctgccact tcgcagactc
     4321 aggcggcctg tcgattagca attttttcaa aattgaatcg ccatgctcgc cagtcttcga
     4381 agcccgaaaa gccaggtcaa aaccgaccct gtgccgttgt tagtcggtgg taataaggaa
     4441 ggattcaaga ggttttccgg cgtcaagttc ttgctgaatt acaaccggca cgcgtccgac
     4501 gcctgaccat tttttatgca cgccgttttc aaaatactga tatttagctt tgcgttttgc
     4561 cgactttttg gcgggagttg tactaaccag ctctgtcggt gaaaagccct ccgattcaat
     4621 cagcgccagc aattcattgc gcttttcttc ccgctgtcgg cgtagctttt cctgctcgat
     4681 atgctctgcg cgtttttcat caagcgctgc tgaaatacgt gcactcagca tctccagaaa
     4741 ggcaaaatca ctttcacgta aaaacttttt cagcgcgcgc tctgaggtta attcgtcctg
     4801 gatacggttc ataatttcag ctccttcggc atagatacgt tcaatccttt cagcggatcg
     4861 cgtggtggat attcaaaacc gagttccccg gtttgcgcgt ttttcagcgt gacgatcatc
     4921 gaataggtgt cgtcgttttt cttcgattta aaacctttaa taacgtcggt tttgcccttg
     4981 ccgattaact tctcggcctg tttttccgtc agctttttgc cgcgcacttc cagccatatt
     5041 ttgaactgac agcccgtaca ggcgcaggtt ttcggcgtca taaccagctc agaaccgcac
     5101 gacggacatt taaccgcgag gcggttaagc gccgttttaa cgggatgttt ttccgtcgcg
     5161 ctgatggcaa tatcggcgct ctcaaccagg cgggaaactt ccgcgtaaag accatcaata
     5221 aagcgctcga tggtcatttc gccggtttca accgccgcct gtttttcagc ccatacggcg
     5281 gtcatatccg gctgcgtcac cgagtcgggc agggcatcaa ttaacgccag cccggcggca
     5341 gttgggataa atttgccctt ttccacggtg atgtaatcgc gggttttcag cgtctcaata
     5401 atagccgcgc gggtcgccgg tgtgccgatg ccgccgtgtt catctttttt gtctttatcc
     5461 ttctccttga gcaatttttt aatgcgcgga tcggcgacaa aatcggccac gcgaacgagg
     5521 gcagcgagca gggtcgcctc ggtaaacagc ggcagcggtt tagttttttt ctccgaaatc
     5581 accacctcgc ggcaggttaa tgtcgccccg gtttgcaggc ggctgatggc ctcaaacgag
     5641 gtgctgtttt cgtcctcctc gtcgtcttct tccgcgtcgt tcaggaacgt cgtaaaaccc
     5701 gcatcggtga ttttacgcgc cgagacttta aacatctcgt caccgcattt aatctccgcg
     5761 atcgactcgt catagcgctt tttcgccacg aactgcgcga gataaaactg cgcgatggtg
     5821 agataaaccg ccttttcctt gtcggtaagg cggcttaaat ccggcatgtt cgtcgtcggg
     5881 ataatggcgg tgtgggcggt gactttttta ctgtcaaacg ccgcactttt ttgcgccgtg
     5941 tcggtggtaa ggccgttaaa aacgtcgagc gtttttaagg ccgccaccac ctgtggcgcg
     6001 tcgttgaatt gttcatcgga cagataagaa cagtcagagc ggttgtaggt aatgtattta
     6061 ctgtccttga gcttctgcgt gatatcgagc gtctgctgcg cggtcagttt gtgcctgcgg
     6121 ttcatataca cctggaggcg cagcaggtta aacggcagcg gtgcggccgt ggtttttccc
     6181 cggtcggtgg cggccgcgag cacgctggcc ggttttccgg ccagagccct cgcgagtcct
     6241 tccccccagg ccttatccgt cagccgcttt ttatcgtcca ccggcgcgta ttcggacgtc
     6301 tgccagcggg cgcagacaac gtccgtaccg ctgataaact gccctgcgag ctggtagtaa
     6361 aacgcctcgc tgtgcgcctg attgtcgcgc caccggcgca caatcattcc cagcaccggc
     6421 gtctggacgc ggccaaccga aatcgtgccc ttatagccct tatcgcgggc tttgagggtg
     6481 taacagcggc tcatactgaa gccaaaaatc gcgtctcctg cgctgcgcat cagggctttc
     6541 aggtacatcc cacgaaaatc gctgttatcg cgcagggagt tgatggcttt tttcaccgct
     6601 ggcgcggtgt tgtcgttaat aagcgcccgt ttcaccggcg cggtgtttcc ggcgaactcc
     6661 agcacctcgt caaccaggag ctgcccctcg tcgtccgggt cgccgcagtg gacaaccatt
     6721 ttgatatccg ggcgccgaat cagcttcacc agggtattag cctgctttgc cacgctcgct
     6781 tttggctgaa gctgtaccgg gtacagcttc agcggcaggg tgtcgagcga ccaggtggcg
     6841 taggccgggt tgatcgtctc cgggggcacc atttccagca cgtggccgac gcagtagctc
     6901 acgatatcgg tcgccgattc ggcatagccg tcgtgccggg tgaagttgcc gcccagcgct
     6961 ttcgcgatat cgctcgcgac tgcgggtttt tctgcaatcc acagtctcat tcttgatctc
     7021 tcctgatttc atccatcgtt tcgaagtaca tcattcgact ttcctgaaag tcgttatcag
     7081 acagactaaa gccatgatcc gccgctactg tcatgaacgt ctcagcatca cggacgctga
     7141 gactggtcag aacatcgtgc aattcgcaca gggcgcgata ttggtagatt tcgtaatgca
     7201 ggccgcagcc gcgatgcgct tctttctcga tgcgagaaat caggctcgcg gcactaacgg
     7261 aatcaccacg cacatgcggc cacggcgggg ctataaatcc ggtttttgtt gccattggag
     7321 tgcatcctta tcgttgagtt tttcccattc cgccgcctcg tcgtcgctca gcggaacggt
     7381 gtaaaagaag accggcatac tgagcacgga gcggaagaac gactgacgtt ctgctggtaa
     7441 tgcctggaag gtcggcgagt gctgcgcggc gagctggacg agttcggacg tgttggcctg
     7501 aaaatgatcc tgtttctggc tgatttccga caaaacgagc agcaccgtct gcaaatcacc
     7561 tgcaggaagc tcctcatctt tcagcagccc ggaaaagtta ttaatagaaa cggtttgatt
     7621 aatcacgtgg tgtctccttt acggctgccg ttttaccaac cgttcatacg gccaaatttc
     7681 gagatgatct tgccgacaat ggcgctgtca gcggacgggc aggcgctcag ttttttctgc
     7741 cgcgctgcgg cggtgtgatc gggtaaaaac gacccctttt ttttcttttt gatgctcatg
     7801 aaatttttgt acatcgggtc acactcggcg ggactgctgc cgtcgagctt tccggccagg
     7861 cacagcacca tcgcgcacgg gtcattcggg tcggcattct ggccatcggc ggcgaacgcc
     7921 ggagaagtaa gggcgcacag cagcgccgcc gtgattacgg tgtgtttcat gatgatttcc
     7981 tctgaaggga agcagggcgc taacgcccgt tggttgttca acgtcggtga cggtctgaag
     8041 gactcagacc gtcaccgacg ttatcggtag aagacgattt ctgaggtgtc gcaggtcagg
     8101 cagccggagc gggggcgaaa ctccaccaca cccttgtagc cgccctcgcg gagccaggtt
     8161 ttggccgcaa agaagcgctg gccgtccggg gcatttacta ccgcgcgcga ggcgtgcgca
     8221 accgcgtacc actgtgcggc cgggtaaatc ccgccgggtt caaaacgcgt aagcacctgc
     8281 gaccagtgcc cttccgtcac cggcgaggcc acgataagac ggttgggttc ccataccggc
     8341 cgggtggtcg ttaccggctg cgccggttgt gaccagtcaa cggccgcagg ctccggtggc
     8401 gaagaacagc ccgccaggag gacgacgatc gccgtcaggc cggtcagttt aggcagcatc
     8461 attgaaactc tccttcatag cctgcatgtg caggggttta aaatagacgt cgctcatgcg
     8521 gcgcacgttg ccgtgaacgt ccacgctcac aatgacgtcg acgcagtgca gtaccttgcg
     8581 cagcagcacg tcaaccggca tgccccggca cgccggatgc tcgtagcagc ggtcaatcag
     8641 cccggagata cactcgcgcg ggctgcccgc gtgcagggag gacataaacc cctcgtgccc
     8701 ggactcaatc agcttgagtg cgtcccacgc ctcgcggcca cgcacctcgg cgagcagggg
     8761 gcggtcgggg ttcatacggt aaatcgcgcg caccaggcga tcagaggtga ctattgcgcc
     8821 ctctccggcg tccgccgggt agaacagatg aacgtagttt ttctggctgt aaaacttcag
     8881 ctccgggttg tcctcgatgg tgataaggcg cagcgtctcg ggaatgtagt gcagcagcat
     8941 cttcatgtag gtggttttcc cagagcctgt ttcccccacc acaaagagcg ttttgccgta
     9001 ctccacgcac ttttccataa aaagcgggat attcccggac aggtatatgc ggctcagctc
     9061 cccgtctttt gattccacct tttctgtacc ctgcacccgg tcataaaacc cggcatcgat
     9121 atacgactga tgggtgcgct ggaagtagtc cggcttacgc agggtgattg agatggtgtt
     9181 acgctcacag gccgggggca ccactatctg gcagcgttct cctgtaggga gcgtggccga
     9241 gaggataggc gacgtttcat caatggtgtc gttgttccac acggccagcg ccctggcaaa
     9301 ggacgagcac tggcgcagcg tgaccggcga ggcgtgcatt tcccacgccc cgttgatttt
     9361 tgtgtgcagc tctccggggc ggttgatggc aatttccgtc agcccctccc ggctcaggta
     9421 gtccccgaac aacagatcgc gcaggtgtga cagcgcgacg ttgccctctg acggggtatc
     9481 agtcagttta tcgtccatcg cttacctcat gcgcagtctg tagacgtctg aaaaatcaat
     9541 gtccgccccc gtgaccaggc caatcacgtc accctggttg aggtacatcg tcggcgggat
     9601 gttgatgctg ttttccagga gcgtttttgc catttccgcc gtggccgcgc gggtgttctc
     9661 cgtgtagtcc gtgttgcgat ccttgcccgg cgacgagccg gaggccgcag cggcaaagtc
     9721 ctgtaccgtg ccgagcagga gggtattacc tatccgctcc agccagtgat tgtctatcca
     9781 gcccgcgacg ccgttctcgc caagcgcccc cacggcctgg ctgtcaacca tcggaataac
     9841 caggcggccg ggttccggcg tgcgcagctc ggtgatggcg aggaacagac gcccctgccc
     9901 gtttttcagc gtgccggtgc ggtacaggcc gcgcgcctca gtgcctgccg ggataaggcg
     9961 cacggcgttg ctggcgctgt aaatgtcctc gctgataagg cagttaaagc gcccgcccac
    10021 gtccgataca aagcgctgca tcatcgcgca ggggatatgc cggtcggccg gaatgtacaa
    10081 atccggatcg aggccgagcc gcttcacgcc ggtaatgcgc gccagcccgt tatcctgcga
    10141 ctgcccggcg ggtgttgcag agcctggcgc ggacgcgctg cccgcaggtg atacgttact
    10201 gctggacgtg cttttcgggg cgctgcttgc agtggttggg gcgctgtctg ccagggcgct
    10261 gaccttgtca aacgtcaggg ggggcggtga tgcctgcggc ggcgcggcgg tatcagtcgg
    10321 agtagtcttc tcctggccgg ttgtgtctgc cagctgtccc agcgggttca gatcccgtcc
    10381 gaggttattg cgctcgcggg cgggggaggt ggcgtgcgat tccggttcat ggctcgcgct
    10441 ttttccgctg ccgtcaccct tcagtttcgc catcaggtaa tccccgccgg tggcgataat
    10501 gaccagtaac accaggctga tggcggccag cacgagcgag cggcggctgc ggcgcttcgc
    10561 gtgctgcacc acctgtggct gtccgggtgg caccgcgcct gcggcgggtt tatcgccgtc
    10621 aatctctgcc cgcgcccgct cgcgggcttc cgcctccagt tcggcggcgg ttttaatgtc
    10681 ttcctgttcg ttcatcattt gctctccagt ttcacatccg gtgagacggt gctgccgttc
    10741 gccacggtga ctttcccgta actgccgttt tcaatcccca cgacctggct gccgtagcgc
    10801 agcacaaagc gtggagaaag agagcgcagt accatcaccc ggtagttacc ctcggtgctg
    10861 aaggtcgggg tgatcacctg ctcgctgccg ccgacgatga cggtcggcga cgggatcagc
    10921 ttctgcggtg aaaagccgag ataggtaaac cggccgtcgt catacgcaaa atccggggtg
    10981 atcgactccg aacctgccgc caccctgcgg gtgtagttcc agttgcgcgg cgcagtggat
    11041 ttcttcaggg caaactgaat gcgttctttc tcctgcgcgg cctgaagctc ctgctgacgg
    11101 gcgaggcggg ctgcgtcgtt ctgcctgcgg gtatcgtcgg ggtagctgaa gcgcaccaca
    11161 aaggccagat ttcccgcctc gcgatcgtta tcgatcacgt tcagcatcag gctgtagtcg
    11221 cgctttgtgg tggtcacaaa caggttggtc tgccagtcct gctgcgtggg ggtgaaaatc
    11281 ttctggacgg ttttgccgtc cgcatccgtt accggctgct tcaccggcgc ggggctaatc
    11341 cccacgcggt tacggtcggg tgtgatcgtc cagccctgcg gaaaaccagc ggtggcggtg
    11401 atcacctcct cgtcggcgtc aaaaaccagc gtcgtgatgt aacccgcccg cgtgttaatc
    11461 actgtcgtgt tctggctgtt gtaggggatg ttctgcatgc ggctgtcata ggcgctgccg
    11521 cgcggtgtcg ccgcccccca tgctgacgct gcggtcagca ggagggcaca catcagtacc
    11581 ttattcatcg cttttctcct taaaccgctg gtaactggtg acgatgaagc cgaacgggtt
    11641 cacctcgcgc tcggaggccg tcattttctt ctgcgggaaa tagcggtagg tcatgcgcac
    11701 cgtccagtaa tcatacgttt cggtgttgtc cttgaccttt cgaatggtgc gcttgatgcg
    11761 cagcatctcc accatgctgg agccggtgcc ctctgacggt acgttggaga ggacttccac
    11821 cgtgactaca taggccgcct gctgaaacac agcgtccggc gcgtccgggc tgttaaacca
    11881 gtcgaggtat tccttgttga cctcgtcgct gttgaacatc tgcgtctctc tgtagtcctg
    11941 ctgaagggcg aaatagttgt acccctcgcg gcgcttcacg taggacgcag tctgcgagag
    12001 attgagcacc tcggaggtgt ccagcttgtc ctcgccgacg gtggtgacgc gctcgatgcg
    12061 cccggtctga cggtcgagcg tgtacagctc cacggtggtg gttttgagcg gtagcagcag
    12121 gataagggcg gcaagggcga agacgagcag cagaccggcg agcgccgcca cgatccagcc
    12181 cgcctttttc atgcgcatat cccgctcaag aatggcgttc tcaaacgagc gcgagtcgct
    12241 cagggctttg gcggtaagtt cagacatggg gggttaactc ctgaataatg gccggggaat
    12301 tgaccggctc cgggttgccc gacacgggcg gaagttgggt gtttcgctgt gcgcagcctg
    12361 caagcaggca cagcatcagc agcgtgatgc cgtatttcat ggtgtttttc ctgtggtaaa
    12421 gacgtgacga agccgcagga cgccgttgcg gcgtcccgca gttcagtcag actgggttat
    12481 ctctggtagc gttcctgcat ccgctgaacg gcggcgcggc tgcggctgcg cgcatcgccg
    12541 gtggcgcggg aggccgtgga ggcagacgag gccgttgcgc tgccggtgct gttaaggttc
    12601 tgtgaggctt tatcccccac cgcaccggct gcggaggagg cggattgacg ggctgaacct
    12661 actcccgacg cggcaagtgc ctgagcggca ccctgtgcgg atgcaccggc aagcgcctcg
    12721 gccagcttat aggtcaggac catcacggcg gcggcacccg ccgtgccgag gaagacctga
    12781 acggccagag tcaggtagtt ggcatcggat gcttttgtgg cgcccgccag gatgtagtcc
    12841 aggtaatcca ttaccaccgt cagggcgatg ccgctgaaca gggatgtaag gatgcagccc
    12901 agaataattc tgagccagtt gctgaacata tcccggaacc acgaccacat caggcagtag
    12961 ataaagatcg gggcggtcgc cagcatcagc agcatcacca gttcggctat caggttaatg
    13021 atggccgtga tgcctaccag taacatgacg cctgcccaga ccagaaactg cgccagagtg
    13081 ccttttagag cgacataggt tgaatcatca taagagtaaa gcttctcccc aagtgcttga
    13141 gccttttccc agacggtatc cagccgcgcc cagatcgacg cgtcgccgct gaatccgtcc
    13201 cgcaggccgg tgatggcatc cgaaaccaga tcgagatagc ctccggcgtt ggtgacgaaa
    13261 atcatgatga tgagcatccg gctcacgtcc cacacgatct cctcgaaggg gcggactagc
    13321 ttgcctgcca tcgtctggta ggcgcgccac gtgatgtaca gcgagagcac cgcgatggag
    13381 aacaggtgtg ccatccaccc ccccgtatcg gcggccacgc tggtgagcac gctgtcaaac
    13441 ccctccatga tgtggctgtt taatccgacg aatattccac tcatggtgcg ttctcctgtc
    13501 gctaattcag gtcagcaata ggctcttgca cgcgagcttt atcaaggtct ttcaggattt
    13561 gcttatatcc ttcggcaaca tcaggtttag ctttttcacg gtgaagggcg gagcgtgcat
    13621 tctggcaatc ggtgggcaca aaattatgat taaaaatgca ctccctgtat ttcgccagca
    13681 tgtcgtcatg gtgtgccata taccagtcta cggaggtttt atcctcgcag ccagtaagcg
    13741 cggccactga ggccagaaca ataaaggtca gtgtcttcag tttcatgggg tcacctgtta
    13801 gttaaggtct gcgattggag ctgtgcgcat ttgctcggcc tgcgcctttc ggcgttgttc
    13861 ggcaagcagc tggttgcgca tctcactttg tttgaccgac atttcccatt gagtggtcag
    13921 ggcgttcagc tggatactcc gcgcggacac tacgttcgcc agatcctgcg attctttact
    13981 gtccttcgca ttctcgatgc gactggcgag attactaata tcgctcagcg tgtcggaaat
    14041 tttttcctgg atgtcagaag tgtcctcgac ggacaccgcc tgattgagga gcatctgttt
    14101 gcagctatcc cggtacgcct cgctggcgtt ctccgggcag ttgtcgaaca gtttgtattt
    14161 gtcatacagc ctgtttagtt ccccagcata agacccgcct gagccggtta acagatcgtt
    14221 taaggaaatc cccctgtttt tcagattttt aatgtcagcc gtcaggtttt ttgcctgatt
    14281 caggaatgcc tggacgtccc gaataccggt ggccgttgcc agctgttgtt tataagcgtt
    14341 catctgcgac tgataatgtt gaaccgtctg ctgccactgc tggagcttct ccgcccactg
    14401 ctggatgttg ctggcgttgg ctacttcatc aaaaacggga ataccggcct gagccgggac
    14461 gtgaaagaga cacaaggcga cggtcagcgc cgtcagggtg gttttcagtt tcatggtgtt
    14521 atccttttgt gattaaacgg cacgtctcag catttcgtct ttccagtctt ccggcttcat
    14581 accctcctgc cagatctcgt caaaaatttt caggttgtcc gcactggcgc tgagcagctt
    14641 cgtgtagcga cctagcccgg agaggtcgag ggtgatttgt gcgatatacg ggcgcacgtc
    14701 accacgccgc agcggagatt tcatgatcac catctggcgg gcgtaggggt cgagggtggt
    14761 cacgatgtcg taaaccgctt tcggcacccg cagcatgtcc acgtagtcgt actccttcgc
    14821 ctgctggttc gccatgaaga tgcgggtact acactgctcg ataatggccg ccgagatctc
    14881 gtgcttgcac atctcctccg gcgactgcgt ggccgggaag aaaatgccgt tgagctttcg
    14941 aatgaccttc agcatgtcga gggcgaattt tgaaaactcc gggtcctgaa gccatttcca
    15001 gaattcatcc atgaaaatga ccaggcggcg gccatcgagg aggctggtca cgcggtagag
    15061 caggtagaag gtgagcgggc tgcgcacgtc tttgtcgttc aggaactccg tgccgtcgat
    15121 gccgaaatta tcaatattgt cgatatcaaa ggtgtccacg tcattatcaa acacccagcc
    15181 aaactccccg ccctgcgccc actgctggag acggatgcgc agcccgttca tccgtgcgtc
    15241 cttcgtcggg gcttcatgca gcacttccag catgcgggtg atgccgttgc gacgaagctc
    15301 aggcgggtag tccagcatga tggtgtccac tgcctcgctg aggcgttctt catcgcgcgg
    15361 gtcgagcggt ttgccgtccc ttgtgcacat cattcgcatc agcttcttga ggaagttgcg
    15421 gttacgtttc gttggagcga gcgcaaacgg gttaaggccg gtcttctcgc ctgaacggaa
    15481 acggaagtac gagccgccct ggaggcgtat cgacatttcc gccgaacggt ctttatcaaa
    15541 gaccaccgag gtgaagcgct ttttcgtcgc ccccggcgca aacgactcag gacgccggta
    15601 tttctgcatc agatgctgga acatcatcag cagggtcgat ttccccgtcc cggtactgcc
    15661 gagcagcatg gtgttacccg gcggcttctc gctgaactcg tcaacatcgg cctggctgtt
    15721 gtgcagattc acgtactgac atcccccgga cggggtcgga acaacggcaa tcgcctcccc
    15781 ccaggcattc ccgtcccgct tgtgcgggtg aaagttgtga aacgacacca tttcggcgta
    15841 gttgaggctg ctcaccggca ccagacgcgg gcgcaggtga tacacacccg gcaactgcgc
    15901 gagatacgcc gccgacagcg actcgcggga cagggtcatg atgaaaccga gatccttcag
    15961 ggtatccgcc agggtgttgg tgtcccggat aacctgctcg ctggttgggg cgctgacaat
    16021 aagcgtctgg tgatatttcc cgaatgaaat aatgccggac tgaagcatgt cgcgggcgac
    16081 cttcaggtct tcctgctggg agatcgcatc atcctcggca gacttcaggc gtttctcgat
    16141 ggtgcggatg cagccccgcg cttcatcctt acccatgcag gtataggact gcgtgaacac
    16201 gtaccggcag cgggcataaa gaagcgagtc atacagcccg gtgatggttt cagtgctgta
    16261 atccttgatt tccagcccgc gaaagtagct ctggccgtcg gtgtggctga cctggccaat
    16321 gtcggtggaa gggaaaatat ccgtgccgcc cagcacgtca taaaacggcg tgcgggtgac
    16381 gcggactttc tgccacctgc cggtgatcag aaagttgtaa aacgccagct gggaggagaa
    16441 cacgtgcccg ttctcctcat aagtgccaag cggccgcgcc gcgtagcgtg acagcgccgt
    16501 gccaatcgct tgccggtgct cctgcatcac ctgcagggca ttgtcgagca gggttttacg
    16561 ttcgcccagg gacttctgtt tcatggtcgc ccgctcaatg ccggtgtgcg gattgaagca
    16621 gacggtgaag aagaggcgac gctggtagaa aggtttgccc tcgcgggagg cgtaaaacag
    16681 acgcgcgatt tcatctgaaa acggaatccc ggattcacat tcaagcgtgt cggtaaattc
    16741 gtcgctcacc agatgggtat agaaggtaac gggtagcccc tcaaaggagc ggataacgtt
    16801 gttgagctgc atgtccaggg tggcgaggta atgttcatcc tcgcattcaa aaatgatacc
    16861 gccgatctcc caggtgctga caaagtcacc ttttttgttc ttcaccacct gctcgtgcag
    16921 gtgggaagac caggggagat agcgggacag cgggatactc tcgttgagcc gcatatgttt
    16981 tataaactcc gtaacgtcta tggcgtcgta ctggtttgcg acaagcgcct ttgtcccgaa
    17041 atgccgggtc gccgcgcggt tgccacgcgt tttaaacgcc agcaacttca ggtcaaagaa
    17101 atgaatgtcc tcttttgcca gccgtctcag ctcgataaag gcaggtatga gaagcaggag
    17161 aagccagtaa aaacgtgacc agacggccgc cagggtaagg atgcacaggg tgaacagcag
    17221 ggggacaagc gggacccccc acagcgccgc cggtcgggtc agcgccttgt tgaggatggt
    17281 catgctgcct ccttaggcgg cgaagtaggc accgaacccg gacgccccgg cgagcaggac
    17341 acccgcccag aacacgcgct ggagatcgtg ccaggccttg ccgtcaacaa gcatcttgta
    17401 ggcgataaag gccaccgcac agctgatagt gacgactgac ataccagtta ggccgaggaa
    17461 gacccagtta atggcgtcac tgcctttctg aaatgcctgg tcagcgctgg ccgccattgc
    17521 tggaacagca gacagggcgg aaaatgcggc aatcaggcgg gttgcagcgg ttttgatagt
    17581 acgtaaagtc atgatctctt ccttaatcag aagacacagc gaggccgccg cgcacgacat
    17641 gtggcggata agcagcgatg ctggtcgggg tggtgtcggg tgccggaatt acgccgcgta
    17701 tgacgctatc cgggtaaaca gtgttgtcgg acgggacggc aggcgcttcc ccggatgagg
    17761 gagatgctgt ctgctgttct gcccgcgttg agggcacaac gtagccaata cgctgtacgt
    17821 agcttgtgtt gccgaacgcg ctttccgggc gctggccggt ttcaaagttg cctgagtaat
    17881 agcagctcag ggcgcgctgt aatgttcctc ctcgttgata gcagtcggtg atgattttcg
    17941 cggcaacagt catgttgtca caggggttaa acatggattc ggcgcttacg ccgaactgcg
    18001 ggaagttggt gctggttatc tgcatcagcc ctactgaata acggtgcttt ttctgcttta
    18061 tggcctccac aatttttagc gcctcgtctt tcgacgaagg cagatgagaa atcacgcggc
    18121 tcctgccgcc ctttaccggg acaatctcag cgatggcata tggatttaag ccggattcgg
    18181 tgcgggtgat gtcgtggatg gtatcggggt gaacggcggg agcacactgc attgcgagct
    18241 gaaacaacgc ggcagtggtt aacatagtca gtcctttctg tagagcagta ccttaccggt
    18301 ggcacagtcc gggttgataa ataactgaat gtcgccctgg ctgttagctt gggcaacacc
    18361 gatttttgta agcgtcattg ccagccgatc acccttcacc gaatacacgt tgtgcgtgta
    18421 ctcggtcatg ggggtgaata cggcttcaag cagtgaggcc ggagcaggcg tgatgtcagg
    18481 cattacattt cctattttcc agcagtgagg tgattgcatc gatacgtcgc agcctgtcgg
    18541 caataagtac ggatacatcg gattttttga gtccctgcgc tttcacagtc agggtagtga
    18601 tttctgcccg cagggctgcc cgttcaatta gcaattctcg gacgttatcc ggcagctccc
    18661 tgagcgacca ggtgccatta atggcacgcg tgatacggtc acgggaggag cgtagcggcg
    18721 gcttgccgag agttgaataa ggattctgac gcagcgcctt acggcggacg gtttgcatgc
    18781 tcatggctgt ttctcctggg tatgagtaag ccacccgcgt gcggtgcaca gtgggagaca
    18841 gggaaggatt aaacgggagg cttaacgata cagaggagtg agagtgttac ccattacagg
    18901 cgcggcacgt catctcttta cgcagtcaca gaaaacacct ccttatcctg accggggaac
    18961 gccgctgcta ggttattaaa gagctaatga tttgattaat acaatagtgc ttataagttg
    19021 ggtattcaat aaaattataa cttatagtat cgatttaaga atgattccaa tttttaggac
    19081 tttttcagct gtgtaaaatg ttggagatgt ttgaaaaaat tgaaaaaaaa tactggcagg
    19141 ataaaatcaa gaaaatctcc aaattcagag taataaaaat gcaagatctc gatgatgcaa
    19201 aagtgataga ggtactggcg aaggtattga ccaaaaaagg tatttccaag gtaaagcaca
    19261 atagttacat aagtaaggtg ctacagatca ctacatcagc gggctataag aagttgaatg
    19321 gtgcgaccaa gtgggaggtt cagcaactca taaaagtagt ccaatccgtt ggcatggata
    19381 tgattgagtt tttccgtatt tattatgagg ataataaaga aattcaagat gcgatctgga
    19441 acgatggccg cagtgaacac tcttgtaagg tatgcctcta tcctgaaggt tcggaagtaa
    19501 acacagaata ctcagcactg aaagtagctg accaatggaa catttttccg acaaatgatt
    19561 taaaagatga actgctttat gaatcaaaac gaagcataga aagtattgtc ataaatccaa
    19621 aggtaattag cagcactaag tatcgaattg cactacttga tgacgatgct aatatcaccg
    19681 aaagcttaaa agaaatcctt tcgaacgatt attatcaagt agatacttat gataacttga
    19741 aaaaacttac tcaagctgtc gcaacaaatc cgtatgacgc atatgttttg gactggatca
    19801 tcggtgatga aacaagcttt aagttggtta aagctataag gtcgtcaaaa aataaaaatg
    19861 caatgataat cgttcttaca ggtcaactat ctggtataaa agatgatgaa attgtaaagt
    19921 caattcatga ttatgacatt gtgggaccat acgaaaagcc gattaaatca ggcatcatca
    19981 aatctaacat tgaaaaatat ttcgccagat agattatgag aaatttact
//
LOCUS       DAECNO010000078        19652 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.078, whole genome shotgun sequence.
ACCESSION   DAECNO010000078 DAECNO010000000
VERSION     DAECNO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.078"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="KKO08_002829"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="KKO08_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBD6319008.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GIIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="KKO08_002830"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="KKO08_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBD6319009.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="KKO08_002831"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="KKO08_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBD6319010.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="KKO08_002832"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="KKO08_002832"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD6319011.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="KKO08_002833"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="KKO08_002833"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBD6319012.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /locus_tag="KKO08_002834"
                     /pseudo
     CDS             complement(5235..5849)
                     /locus_tag="KKO08_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="KKO08_002835"
     CDS             complement(5911..6954)
                     /locus_tag="KKO08_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBD6319013.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7200..7652)
                     /locus_tag="KKO08_002836"
     CDS             complement(7200..7652)
                     /locus_tag="KKO08_002836"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD6319014.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="KKO08_002837"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="KKO08_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBD6319015.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="KKO08_002838"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="KKO08_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBD6319016.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="KKO08_002839"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="KKO08_002839"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBD6319017.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="KKO08_002840"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="KKO08_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBD6319018.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="KKO08_002841"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="KKO08_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12057..12578)
                     /locus_tag="KKO08_002842"
                     /pseudo
     CDS             complement(12057..12578)
                     /locus_tag="KKO08_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            12962..13702
                     /locus_tag="KKO08_002843"
     CDS             12962..13702
                     /locus_tag="KKO08_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBD6319019.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(13673..14440)
                     /locus_tag="KKO08_002844"
     CDS             complement(13673..14440)
                     /locus_tag="KKO08_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBD6319020.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(14646..15224)
                     /gene="lpcA"
                     /locus_tag="KKO08_002845"
     CDS             complement(14646..15224)
                     /gene="lpcA"
                     /locus_tag="KKO08_002845"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBD6319021.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            15464..17908
                     /gene="fadE"
                     /locus_tag="KKO08_002846"
                     /pseudo
     CDS             15464..17908
                     /gene="fadE"
                     /locus_tag="KKO08_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
     gene            complement(17951..18397)
                     /gene="ivy"
                     /locus_tag="KKO08_002847"
     CDS             complement(17951..18397)
                     /gene="ivy"
                     /locus_tag="KKO08_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBD6319022.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            18578..19348
                     /gene="yafV"
                     /locus_tag="KKO08_002848"
     CDS             18578..19348
                     /gene="yafV"
                     /locus_tag="KKO08_002848"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBD6319023.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            19458..19652
                     /locus_tag="KKO08_002849"
                     /pseudo
     CDS             19458..19652
                     /locus_tag="KKO08_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         4787 a         5052 c         5069 g         4744 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcattatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggga tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggatgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 aaaatcatct cttccgtcaa aatcctgcgc gcgcctaaaa aatggtcctg ccagaaaggt
    12121 tcggctaacc ggcttacccg aatggtttcc ccggtgcgca gtgactcaat aaactgaccg
    12181 tcgcccagat acacccccat atggtcggca atctcgcggc tgtggatatg gaaaaatagc
    12241 aaatctcccc ggcgcaggtc gttattcgcc acaatcgttg cccgacggta gtggtacatc
    12301 tcattggccg tgcgcgggag tttcgcctca aggatcttgt tgtaggcata aaaaactaac
    12361 ccactacaat cgaagccttg atcgggccgc gtgccgcccc agacgtaagg cttgccaagc
    12421 tgctgctcca ggcggtgaat cgccacttcg gtgatgttat gcaggtgatc gctgcttaaa
    12481 aagtggttgt tttccgccag cgcctgccag cgtctgtcac tcgccttgag cggcgcagga
    12541 aaccattctg gatgctgttt tatctgctcc cggttgtggt ggcgctggcg agtaaaggga
    12601 gcaaaacgca taacgaaaac acgcctggat gcaaaaatct gccgagcaag aacgaagaca
    12661 tgaaagacat aaaccggctg aatgaaggtg agaattttaa aagaccggag gagtaaacgg
    12721 gattattaat tgcttatttg catattacta ttgagatttc ataatattag gaaataaatg
    12781 atacatgcgc ttttcggact attgaaaact gcccctgtgc taaatttgta aacccagcgc
    12841 cgacaggctt gcgtaaaact cttttggtga taatgtgagc cttccaaatc cagactattc
    12901 cgatttggga tccattttgc cgtcctggcg tgtgtaaccg ttttatcaag gaataagcag
    12961 tatgcgtaaa atcgcattaa ttcttgcgat gttgttgatc ccgtgcgttt cgtttgccgg
    13021 tttgctgggc agcagtagct cgaccacgcc agtgagcaaa gagtataagc agcagttgat
    13081 gggatcccct gtctacatcc agatcttcaa ggaagaacgt acgctcgatc tttacgtcaa
    13141 aatgggcgag caatatcaac tgctcgacag ttataaaatc tgtaaatatt ccggcggctt
    13201 agggccaaaa cagcgtcagg gcgatttcaa aagcccggaa gggttttata gcgtccagcg
    13261 taatcagtta aaaccagaca gccgttacta caaagcgatt aatattggat tccccaatgc
    13321 ttatgaccgt gcgcatggtt atgaagggaa atacctgatg atccacggcg attgtgtatc
    13381 catcggctgc tacgcaatga ccaatcaggg tattgatgag atcttccagt tcgttactgg
    13441 tgcgctggtg tttggtcagc cgagcgtgca ggtgagtatt tacccattcc gcatgaccga
    13501 cgccaatatg aagcgccata aatattccaa ctttaaggac ttctgggagc agctgaagcc
    13561 gggctacgac tactttgagc aaacccgtaa gccaccgacc gtttctgtgg ccaatggccg
    13621 ttacgtggtc agcaagccgt taagccacga agtggtgcag ccacaactgg catcaaacta
    13681 cacgctcccc gaggcaaaat aagtgccatt cgcctggcat aattttttgc caggtttcat
    13741 tgcccgtcag cggctgtgtc gcaatcaccg tgaccacatc attcggtgtg gtctgcgagc
    13801 tgaagtcgat ttccacatcc tgatccagca acgttgccac gccaaacggc gcgcggcggg
    13861 tgatccagtg taaattagtc gagcaatacg ccattacata gcgcccgtcc gaaagcagca
    13921 tgttgaaaac gcccttcgcc cgcagttcat ccgccagtga ggcgatatat ttaaacactg
    13981 ccgccatgtt gcccggcgtg cgcgggtaac gctgcgttaa tttatgcagc agccagcaaa
    14041 aggctttttc gctgtcggtt tcaccgaccg ggcggaagtt gccggtttcc agtgatttgt
    14101 agcccgtcag ttgtccgtta tgggcataag tccagttacg cccccataac tcgcgggtaa
    14161 atgggtgagt attttccagc gccacctcgc cccgattagc ctggcgaata tgagccacca
    14221 ccgaacagga ttttatcggg tagtcctgga caagtttggc gatgggggaa ttaaagctgg
    14281 gttgtggatc tttaaatgtg cgacagcctt taccttcgta aaaggtaatg ccccagccat
    14341 ctttatgtgg cccggttcca ccaccacgct gtacaagtcc ggtgaaacta aagcagatat
    14401 cggtagggac gttggcgctc atcccgagca attcgcacat gggtaacctc cacaacagcg
    14461 ggagcgcatg gaccccggcg aagattgatt taagaatttt caggtcggat gcggcgtaaa
    14521 cgaattattc ggcctacggt tcgggtacag atttgtaggc atgataagac gcgtcagcat
    14581 cgcatcaggc atcagcgcac aagtgccgga tgcggcgtaa acgccttatc cggcctacgc
    14641 cagacttact taaccatctc tttttcaatt aactggatca ggatatggat cactttaatg
    14701 tgaatctcct gaatgcggtc ggcataacca aagtgcggaa cgcgaatttc gatatccgcc
    14761 gtgccagcca ttttaccgcc gtctttaccg gtcagggtga tcactttcat ccccttctca
    14821 cgcgccgctg cgatcgcttt gatcacgttt gcagagttac cggaggtgga gatccccagc
    14881 agtacatcgc cttcgcgacc taccgcttca acgtagcggg agaaaatatc attgaaaccg
    14941 aaatcattac cgacgcagga aatatgacta acgtcagaaa tagcaatcgc cgggtagccc
    15001 ggacggtttt cacggtagcg accggtcaac tcttcggcaa agtgcatagc gtcgcaatgg
    15061 gaaccgccgt tgccgcagga aagcactttg ccaccggctt taaagctgtc tgctaacagg
    15121 accgccgcgc gctgaatggc gtgaatattg gcgtcatctt ttaaaaagtt agccagcgtt
    15181 tccgccgctt cgttcagttc gttacgaata agatcctggt acatgaggat atccttcagc
    15241 ataaatgtaa tagacaaaat gcagtgtacc ggataccgcc aaaagcgaga agtacgggca
    15301 ggtgctatga ccaggacttt ttgacctgaa gtgcggataa aaacagcaac aatgtgagct
    15361 ttgttgtaat tatattgtaa acacattgct aaatgttttt acatccacta caaccatatc
    15421 atcacaagtg gtcagacctc ctacaagtaa ggggcttttc gttatgatga ttttgagtat
    15481 tctcgctacg gttgtcctgc tcggcgcgtt gttctatcac cgcgtgagct tatttatcag
    15541 cagtctgatt ttgctcgcct agacagccgc cctcggcgtt gctggtctgt ggtcggcgtg
    15601 ggtactggtg cctctgacca ttatcctcgt gccatttaac tttgcgccta tgcgtaagtc
    15661 gatgatttcc gcgccggtat ttcgcggttt ccgtaaggtg atgccgccga tgtcgcgcac
    15721 tgagaaagaa gcgattgatg cgggcaccac ctggtgggag ggcgacttgt tccagggcaa
    15781 gccggactgg aaaaagctgc ataactatcc gcagccgcgc ctgaccgctg aagagcaagc
    15841 gtttctcgac ggcccggtag aagaagcctg ccggatggcg aatgatttcc agattactca
    15901 tgagctggcg gatctgccgc cggagttgtg ggcgtacctt aaagagcatc gcttcttcgc
    15961 gatgattatc aaaaaagagt acggcggact ggagttctca gcttatgccc agtctcgtgt
    16021 gctgcaaaaa ctctctggcg tgagcgggat cctggcgatt accgtcggcg tgccaaactc
    16081 attaggcccg ggcgaactgt tgcaacatta cggcactgac gagcagaaag atcactatct
    16141 gccgcgtctg gcgcgtggtc aggagatccc ctgctttgca ctgaccagcc cggaagcggg
    16201 ttccgatgcg ggcgcgattc cggacaccgg gattgtctgc atgggcgaat ggcagggcca
    16261 gcaggtgctg gggatgcgtc tgacctggaa caaacgctac attacgctgg caccgattgc
    16321 gaccgtgctt gggctggcgt ttaaactctc cgacccggaa aaattactcg gcggtgcaga
    16381 agatttaggc attacctgtg cgctgatccc aaccaccacg ccgggcgtgg aaattggtcg
    16441 tcgccacttc ccgctgaacg taccgttcca gaacggaccg acgcgcggta aagatgtctt
    16501 cgtgccgatc gattacatca tcggcgggcc gaaaatggcc gggcaaggct ggcggatgct
    16561 ggtggagtgc ctctcggtag gccgcggcat caccctgcct tccaactcaa ccggcggcgt
    16621 gaaatcggta gcgctggcaa cgggcgcgta ttctcacatt cgccgtcagt tcaaaatctc
    16681 tatcggtaag atggaaggga ttgaagagcc gctggcgcgt attgccggga acgcttatgt
    16741 aatggatgct gcggcatcgc tgattaccta cggcattatg ctcggcgaaa aacctgccgt
    16801 gctgtcggct atcgttaagt atcactgtac ccaccgcggg cagcagtcga ttattgatgc
    16861 gatggatatt accggcggta aaggcattat gctcgggcaa agcaacttcc tggcgcgtgc
    16921 ttaccagggc gcaccgattg ccatcaccgt tgaaggggct aacattctga cccgcagcat
    16981 gatgatcttc ggacaaggag cgattcgtta ccatccgtac gtgctggaag agatggaagc
    17041 ggcgaagaac aatgacgtca acgcgttcga taaactgttg ttcaaacata tcggtcacgt
    17101 cggtagcaac aaagttcgca gcttctggct gggcctgacg cgcggtttaa ccagcagcac
    17161 gccaaccggc gatgccacta aacgctacta tcagcacctg aaccgcctga gcgccaacct
    17221 cgccctgctt tctgatgtct cgatggcact gctgggcggc agcctgaaac gtcgtgagcg
    17281 tatctcggca cgtctggggg atattttaag ccagctttac ctcgcctctg ccgttctgaa
    17341 gcgttatgac gacgaaggcc gtaatgaagc cgatctgccg ctggtgcact ggggcgtaca
    17401 ggatgcgctg tatcaggcag aacaggcaat ggacaattta ctgcaaaact tcccgaaccg
    17461 cgtggttgcc gggctgctga atgtggtgat cttcccgacc ggacgtcatt atctggcacc
    17521 gtctgacaag ctggatcata aagtggcgaa gattttacaa gtgccgaacg ccactcgttc
    17581 acgtattggt cgcggtcagt acctgacgcc aagcgagcat aacccggttg gtttactgga
    17641 agaggcgttg gtggatgtga ttgccgccga cccgattcat cagcggatct gtaaagagct
    17701 gggtaaaaat ctgccattta cccgtctgga tgaactggcg cacaacgcgc tgacgaaggg
    17761 gctgattgat aaagatgaag ccgctattct ggtgaaagcg gaagaaagcc gtctgcgcag
    17821 tattaacgtt gatgactttg atccggaaga gctggcgacg aagccggtaa agttgccgga
    17881 gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga gccgaaaggc tccgtttctt
    17941 tatccgctaa ttatttaaaa ttaaagccat ccggatggtt ttccaggctg ccggtcaacg
    18001 ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc gttcacattc agccaggtga
    18061 gtttctcttg cgacgttttc tcatcaatag ccgagaacag ccccgtcatc tgattagatt
    18121 tctcggacca catcacagcg atacgttgcg agccacagtc atgcggtttg cacgcgctca
    18181 tcacctgata cgtctcatct cccaacgtta cggtttgtgc gggagtataa gtaccgcctt
    18241 tcatcaccca tgcaggtagc ttatgccctt gcaccatctg attaaacgca gctttggtgg
    18301 tttcgccctt tgcaaggctg ctaatggtta aatcatcctg cgccattgca ctggtggcga
    18361 tgaccagtgc ggcgactgtc gttattgcct taaacatcat tcctcccgag cttatcctgc
    18421 ccatgttatt aacctcctaa agcctggcga taacacgata atttgcaaat cagatatttc
    18481 caatatcagc atttgttatc aatttattct gcgctaaggc aactgcctga taaggtgtta
    18541 aagttataaa acccttcgca taaaggagtt gtacaccgtg cctagtttga agattacgct
    18601 tttgcagcaa ccgttggtgt gggtggatgg tcctgccaac ctgcgccatt ttgatcgtca
    18661 actggaaggt attaccgggc gcgatgtgat cgttctaccg gagatgttta ccagcggctt
    18721 tgccatggaa gcggcagctt cgtcgcttgc acaagatgac gtagtgaact ggatgacagc
    18781 taaggcgcag cagtgcaatg cgctgattgc aggcagtgtt gcattacaaa cggagtctgg
    18841 ttcggttaac cgctttttgc tggttgagcc gggcggcacg gtacattttt atgataagcg
    18901 tcatctgttc cgcatggcag atgagcatct gcattataaa gcgggcaatg cgcgagtgat
    18961 tgtggaatgg cgcggctggc gtattttgcc gctggtgtgc tacgacttac gttttccagt
    19021 gtggtcgcgc aatctcaacg attatgacct cgccctgtac gtcgccaact ggccagctcc
    19081 gcgctctctg cactggcagg cattgctgac ggcccgcgcg attgagaatc aggcgtatgt
    19141 ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc cattatcgcg gtgacagccg
    19201 ggtgattaat ccgcaaggcg agattatcgc taccgccgac gcgcataagg caacgcgtat
    19261 tgatgcggag ctgtcgatgg tggcattgcg ggaatataga gaaaagtttc cggcatggcg
    19321 ggatgcggat gagtttaggt tgtggtgagt gggggttgtg tttagggccg ggggctgggc
    19381 tagattacga aagcccctaa cttaaacaca atattacgcc agaagtcacc atttccaaaa
    19441 cacagcgtcg ctgtaccgtg ggttatctgc ccaaccgcag cgacacgccg gtgatcaaaa
    19501 tcagcgccaa atggctgaga aaagcgggat ttgagatcgg cacgggcgtt accgtgaaga
    19561 tctcggaggg atgtttgatc ctgcttggga taacaacgaa gtacaggaac tgcgcaggga
    19621 actttatcag gttaaacagc cagttaaggg ga
//
LOCUS       DAECNO010000079        19375 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.079, whole genome shotgun sequence.
ACCESSION   DAECNO010000079 DAECNO010000000
VERSION     DAECNO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.079"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="KKO08_002850"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="KKO08_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBD6319024.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="KKO08_002851"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="KKO08_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBD6319025.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KKO08_002852"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="KKO08_002852"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBD6319026.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="KKO08_002853"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="KKO08_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBD6319027.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="KKO08_002854"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="KKO08_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBD6319028.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="KKO08_002855"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="KKO08_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBD6319029.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="KKO08_002856"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="KKO08_002856"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBD6319030.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="KKO08_002857"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="KKO08_002857"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBD6319031.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="KKO08_002858"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="KKO08_002858"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBD6319032.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="KKO08_002859"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="KKO08_002859"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBD6319033.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="KKO08_002860"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="KKO08_002860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBD6319034.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="KKO08_002861"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="KKO08_002861"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBD6319035.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="KKO08_002862"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="KKO08_002862"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBD6319036.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="KKO08_002863"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="KKO08_002863"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBD6319037.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="KKO08_002864"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="KKO08_002864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBD6319038.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="KKO08_002865"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="KKO08_002865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBD6319039.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="KKO08_002866"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="KKO08_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBD6319040.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="KKO08_002867"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="KKO08_002867"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBD6319041.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DAECNO010000080        19263 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.080, whole genome shotgun sequence.
ACCESSION   DAECNO010000080 DAECNO010000000
VERSION     DAECNO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.080"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            32..331
                     /locus_tag="KKO08_002868"
     CDS             32..331
                     /locus_tag="KKO08_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBD6319042.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            328..2076
                     /locus_tag="KKO08_002869"
     CDS             328..2076
                     /locus_tag="KKO08_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD6319043.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2365..2643
                     /locus_tag="KKO08_002870"
     CDS             2365..2643
                     /locus_tag="KKO08_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319044.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2640..2945
                     /locus_tag="KKO08_002871"
     CDS             2640..2945
                     /locus_tag="KKO08_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319045.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2955..3116
                     /locus_tag="KKO08_002872"
     CDS             2955..3116
                     /locus_tag="KKO08_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBD6319046.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3258..3482
                     /locus_tag="KKO08_002873"
     CDS             3258..3482
                     /locus_tag="KKO08_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319047.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3783..4940
                     /locus_tag="KKO08_002874"
                     /pseudo
     CDS             3783..4940
                     /locus_tag="KKO08_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4980..5552
                     /locus_tag="KKO08_002875"
     CDS             4980..5552
                     /locus_tag="KKO08_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBD6319048.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5554..6765
                     /locus_tag="KKO08_002876"
     CDS             5554..6765
                     /locus_tag="KKO08_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBD6319049.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6762..7100
                     /locus_tag="KKO08_002877"
     CDS             6762..7100
                     /locus_tag="KKO08_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBD6319050.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7097..7393
                     /locus_tag="KKO08_002878"
     CDS             7097..7393
                     /locus_tag="KKO08_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBD6319051.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7393..7833
                     /locus_tag="KKO08_002879"
     CDS             7393..7833
                     /locus_tag="KKO08_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD6319052.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7817..7999
                     /locus_tag="KKO08_002880"
     CDS             7817..7999
                     /locus_tag="KKO08_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319053.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8055..8345
                     /locus_tag="KKO08_002881"
     CDS             8055..8345
                     /locus_tag="KKO08_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBD6319054.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8365..10026
                     /locus_tag="KKO08_002882"
     CDS             8365..10026
                     /locus_tag="KKO08_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBD6319055.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10040..10321
                     /locus_tag="KKO08_002883"
     CDS             10040..10321
                     /locus_tag="KKO08_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319056.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="KKO08_002884"
     CDS             complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="KKO08_002884"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBD6319057.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="KKO08_002885"
     CDS             complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="KKO08_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBD6319058.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13418..14788)
                     /gene="purB"
                     /locus_tag="KKO08_002886"
     CDS             complement(13418..14788)
                     /gene="purB"
                     /locus_tag="KKO08_002886"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBD6319059.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="KKO08_002887"
     CDS             complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="KKO08_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBD6319060.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="KKO08_002888"
     CDS             complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="KKO08_002888"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBD6319061.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="KKO08_002889"
     CDS             complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="KKO08_002889"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBD6319062.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="KKO08_002890"
     CDS             complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="KKO08_002890"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBD6319063.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17925..19175
                     /gene="icd"
                     /locus_tag="KKO08_002891"
     CDS             17925..19175
                     /gene="icd"
                     /locus_tag="KKO08_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBD6319064.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5016 a         4728 c         5159 g         4360 t
ORIGIN      
        1 ataaagaaca acacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
       61 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
      121 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      181 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      241 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      301 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      361 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      421 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      481 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      541 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      601 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      661 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      721 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      781 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      841 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      901 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      961 gcgttaaaag ttattgccga acccatgggc gagccgtccg gcgatttgct gccgttcgcc
     1021 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1081 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1141 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1201 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1261 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1321 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1381 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1441 ggaacgggca tcaagaaaat cacgggcggt gaccccgcgg aaattaaccc gaaatacgaa
     1501 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1561 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1621 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1681 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1741 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1801 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1861 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1921 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1981 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     2041 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2101 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2161 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2221 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2281 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2341 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2401 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2461 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2521 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2581 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2641 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2701 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2761 catcatgggc gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2821 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2881 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2941 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcggctggtg ggcgacccgc
     3001 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3061 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3121 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3181 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3241 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3301 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3361 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3421 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3481 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3541 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3601 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3661 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3721 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3781 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3841 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcacaaaat
     3901 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
     3961 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     4021 tacgcaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4081 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4141 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4201 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4261 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4321 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4381 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4441 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4501 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4561 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4621 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4681 accgtttaca gacgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4741 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4801 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4861 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4921 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4981 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     5041 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5101 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5161 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5221 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5281 cagggcgcga cgttatcgaa ctggttaagc gcggggacat ctcggggatg agctttggct
     5341 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5401 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5461 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5521 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5581 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5641 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5701 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5761 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5821 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5881 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5941 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     6001 atcactgacg cctacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt
     6061 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6121 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6181 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6241 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6301 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6361 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6421 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6481 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6541 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6601 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6661 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6721 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6781 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6841 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6901 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6961 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     7021 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7081 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7141 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7201 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7261 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7321 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7381 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7441 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7501 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7561 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7621 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7681 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7741 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7801 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7861 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7921 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7981 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     8041 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8101 ttgcggaaca gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8161 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8221 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8281 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8341 cgtaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8401 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8461 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8521 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8581 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8641 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8701 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8761 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8821 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8881 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8941 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     9001 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9061 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggtcaac
     9121 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9181 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9241 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9301 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9361 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9421 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9481 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9541 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9601 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9661 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9721 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9781 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9841 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9901 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9961 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
    10021 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10081 tggaatcagc cagcaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10141 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10201 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10261 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10321 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10381 ctgttaacca gcaggccgga atatattcag aaattacgaa attttcgcag tttaattact
    10441 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10501 cgaggtaact gtttgatatt cccgatagcg caaaaatgcg cggtcgggca gaatgaccac
    10561 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10621 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acatcatttt
    10681 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10741 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10801 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10861 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10921 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10981 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    11041 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11101 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11161 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11221 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11281 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11341 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11401 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11461 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11521 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11581 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11641 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11701 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11761 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11821 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11881 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11941 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    12001 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12061 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12121 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12181 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12241 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12301 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12361 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12421 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12481 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12541 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12601 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12661 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12721 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12781 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12841 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12901 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12961 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    13021 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13081 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13141 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13201 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13261 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13321 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13381 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13441 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13501 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13561 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13621 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13681 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13741 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13801 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13861 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13921 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13981 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    14041 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14101 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14161 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14221 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14281 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14341 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14401 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14461 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14521 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14581 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14641 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14701 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14761 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14821 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14881 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14941 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    15001 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15061 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15121 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15181 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15241 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15301 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15361 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15421 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15481 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15541 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15601 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15661 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15721 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15781 gtctttgtcc accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15841 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15901 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15961 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    16021 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16081 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16141 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16201 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16261 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16321 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16381 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16441 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16501 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16561 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16621 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16681 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16741 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16801 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16861 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16921 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16981 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    17041 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17101 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17161 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17221 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttgcgttca
    17281 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17341 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17401 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17461 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17521 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17581 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17641 ggctggtttt catgtttacg cctggtagaa cgttgtgagc tgaatcgctt aacctggtga
    17701 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17761 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17821 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17881 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17941 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    18001 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18061 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18121 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18181 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18241 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18301 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18361 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18421 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18481 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18541 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18601 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18661 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18721 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18781 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18841 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18901 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18961 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    19021 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19081 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19141 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19201 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19261 tat
//
LOCUS       DAECNO010000081        18096 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.081, whole genome shotgun sequence.
ACCESSION   DAECNO010000081 DAECNO010000000
VERSION     DAECNO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.081"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..134)
                     /locus_tag="KKO08_002892"
     CDS             complement(<1..134)
                     /locus_tag="KKO08_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6319065.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="KKO08_002893"
     CDS             complement(236..1216)
                     /locus_tag="KKO08_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBD6319066.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="KKO08_002894"
     CDS             complement(1224..1934)
                     /locus_tag="KKO08_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBD6319067.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="KKO08_002895"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="KKO08_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBD6319068.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="KKO08_002896"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="KKO08_002896"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBD6319069.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="KKO08_002897"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="KKO08_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBD6319070.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="KKO08_002898"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="KKO08_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBD6319071.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /locus_tag="KKO08_002899"
     CDS             8511..8621
                     /locus_tag="KKO08_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBD6319072.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="KKO08_002900"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="KKO08_002900"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBD6319073.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="KKO08_002901"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="KKO08_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBD6319074.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="KKO08_002902"
     CDS             11503..12135
                     /locus_tag="KKO08_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBD6319075.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="KKO08_002903"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="KKO08_002903"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBD6319076.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15667..16599
                     /locus_tag="KKO08_002904"
     CDS             15667..16599
                     /locus_tag="KKO08_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD6319077.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="KKO08_002905"
     CDS             complement(16640..18070)
                     /locus_tag="KKO08_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBD6319078.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4358 a         4575 c         4361 g         4802 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcag
//
LOCUS       DAECNO010000082        17993 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.082, whole genome shotgun sequence.
ACCESSION   DAECNO010000082 DAECNO010000000
VERSION     DAECNO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.082"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            38..1129
                     /locus_tag="KKO08_002906"
                     /pseudo
     CDS             38..1129
                     /locus_tag="KKO08_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1275..2312)
                     /gene="iap"
                     /locus_tag="KKO08_002907"
     CDS             complement(1275..2312)
                     /gene="iap"
                     /locus_tag="KKO08_002907"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBD6319079.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2564..3472
                     /gene="cysD"
                     /locus_tag="KKO08_002908"
     CDS             2564..3472
                     /gene="cysD"
                     /locus_tag="KKO08_002908"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBD6319080.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3474..4901
                     /gene="cysN"
                     /locus_tag="KKO08_002909"
     CDS             3474..4901
                     /gene="cysN"
                     /locus_tag="KKO08_002909"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBD6319081.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4901..5506
                     /gene="cysC"
                     /locus_tag="KKO08_002910"
     CDS             4901..5506
                     /gene="cysC"
                     /locus_tag="KKO08_002910"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBD6319082.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5556..5879
                     /locus_tag="KKO08_002911"
     CDS             5556..5879
                     /locus_tag="KKO08_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBD6319083.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            6073..6384
                     /gene="ftsB"
                     /locus_tag="KKO08_002912"
     CDS             6073..6384
                     /gene="ftsB"
                     /locus_tag="KKO08_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBD6319084.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6403..7113
                     /gene="ispD"
                     /locus_tag="KKO08_002913"
     CDS             6403..7113
                     /gene="ispD"
                     /locus_tag="KKO08_002913"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBD6319085.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7113..7592
                     /gene="ispF"
                     /locus_tag="KKO08_002914"
     CDS             7113..7592
                     /gene="ispF"
                     /locus_tag="KKO08_002914"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBD6319086.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7589..8638
                     /gene="truD"
                     /locus_tag="KKO08_002915"
     CDS             7589..8638
                     /gene="truD"
                     /locus_tag="KKO08_002915"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBD6319087.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8619..9380
                     /gene="surE"
                     /locus_tag="KKO08_002916"
     CDS             8619..9380
                     /gene="surE"
                     /locus_tag="KKO08_002916"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBD6319088.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9374..10000
                     /gene="pcm"
                     /locus_tag="KKO08_002917"
     CDS             9374..10000
                     /gene="pcm"
                     /locus_tag="KKO08_002917"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBD6319089.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10140..11279
                     /gene="nlpD"
                     /locus_tag="KKO08_002918"
     CDS             10140..11279
                     /gene="nlpD"
                     /locus_tag="KKO08_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBD6319090.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11342..12334
                     /gene="rpoS"
                     /locus_tag="KKO08_002919"
     CDS             11342..12334
                     /gene="rpoS"
                     /locus_tag="KKO08_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBD6319091.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(12454..12861)
                     /locus_tag="KKO08_002920"
     CDS             complement(12454..12861)
                     /locus_tag="KKO08_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBD6319092.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            13008..13601
                     /locus_tag="KKO08_002921"
     CDS             13008..13601
                     /locus_tag="KKO08_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBD6319093.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13601..14548
                     /locus_tag="KKO08_002922"
                     /pseudo
     CDS             13601..14548
                     /locus_tag="KKO08_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14821..15384)
                     /locus_tag="KKO08_002923"
     CDS             complement(14821..15384)
                     /locus_tag="KKO08_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319094.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15549..15800
                     /locus_tag="KKO08_002924"
     CDS             15549..15800
                     /locus_tag="KKO08_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6319095.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15802..17898
                     /locus_tag="KKO08_002925"
     CDS             15802..17898
                     /locus_tag="KKO08_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6319096.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4456 a         4388 c         4817 g         4332 t
ORIGIN      
        1 ttattggctg atatatatca tagatagttt ttaaaatatg cgtaaatatc ctttaagttt
       61 actgaaggat aaaaatattg tgactttctt tgatttctgg ggaaaaaacc gacgtggcga
      121 gaaagagggt atcgacgact atcaccttct ttgctggcat ttgctggatg tggccgcaat
      181 gggctattta atggttaaaa gaaattgctt cgggctggct gattattttc gtcaattagg
      241 gatttctgac aaggaacagg cagctcaatt tttcgcctgg ttgctgtgct ggcacgatat
      301 tggaaaattt gcccgctctt ttcaacaact ttacctgccc cctgaattca agattcagga
      361 aggtgccaga aaaaattacg aaaaaatctc tcattcaacg ctgggctact ggttgtggaa
      421 tcattattta agtgaatgtc aggagttact tccttcatct tcgctctctc cgcgcaaact
      481 taggcgagtg atagaaatgt ggatgcccgt gacgacaggg catcatggtc ggccccctga
      541 tcgtatggat gaattggata attttctgcc tgaagacaaa gctgcagcac gagattttct
      601 ccttgaaatc aagccactgt ttccgcgaat agagatcccc acattctggg atgatgacga
      661 gggcgttgaa cttataaaac aactttcctg gtatatctct gcaaccgtcg tacttgcaga
      721 ctggacaggg tcgtcaacgc gattttttcc acgcgtcgca caggcaatgg atattgaaga
      781 ttactggcaa aaggctttag ttcaggctca aaacgcatta accgtctttc cgccacaagc
      841 agaaaccgcg cctttcaccg gaattaatac gctgtttcct tttattgaga atacgacacc
      901 gttacagcaa aaggtgctgg atctggatat caatcagcca ggaccacagt tgtttattct
      961 ggaagatgtg accggcgcag gtaaaacaga agcggcgctt atcctggcgc acaggttgat
     1021 ggctgcgggt aacgctcagg ggctgttttt tggcctgcca acaatggcaa cggccaatgc
     1081 catgtacgat cgactggtca aaacctggct tgctttctat tcgccagagg tgttccccgc
     1141 tccagcggag ataaaccggc gcaatacggt gcggttttcc cggaagtaaa ccggacgcac
     1201 tggatgcgat gatgggtatc acttagaatt ccccgcccct gcggtagaac tcccagctcc
     1261 cattttcaaa cccatcaaga cgccttcgcc agctccttca ccaacggtag cattatccgc
     1321 ataacgtcac ggcagcgacg ttctattctt ccaggaagtg ccttatcaat atgttgttga
     1381 ttatccagtc ttacgtcatg ccagctattt cccgccggga aggcaggtgt ttttgcgcgt
     1441 tgctgataac catccttatt tccaagattc cagttagtcg cttccaccga aagtacagca
     1501 atgcccgctt tgtcgaatat ttctgcgtca ttacaacacc cagtgccttt cggataattt
     1561 ttattcaaac ccggattggt cgttgcggct attccgtgac tgcgtgcaat tgccagcgcc
     1621 ctgtcgcgcg ttaatttcct tactgcttca ggggttttta caccgctgtt gaaatacaat
     1681 ttatcgccaa caattaagtt atcgagatta atcaccagca gcgtattttt cttttcggtg
     1741 tcactcatcc gcttgagtaa attctcagcg cctaattttc cttcctcttc gccgctggtc
     1801 gccacaaatc gaataccata ctcggtaggc gtatttttca ggcgttctgc cagttccagc
     1861 atgacaccta aacctgcggc gttatcatct attccttgta acgtcagtcc gccgagattg
     1921 gcatctgcgt cagcatcgct cagcggggcg taagtatcca gatgcgccat aatgatgatc
     1981 tgctgcggcg ctttgccttc atgagcggca atcaccgtac ttcctgtcac gttatgccag
     2041 ttcttgcgat tatcgcgggc ggtataaata taccgactat taaatgtccg aatatcactg
     2101 cgataaccca tttgctgaaa ctgttggcga atataatcgg cagataacat ttctgctgga
     2161 gttccggtca tgcgtcccgg aaagaaagta gcaatatgtc gtgcctgggt attagcaaaa
     2221 tcgccaggtt taggtgacga ggcgtgtacg gggagaataa agcatacgcc gagcgccagg
     2281 gcagcggtac ggtggcgcaa tgcggaaaac atagtgagtc cttaaatacc atgcaaattt
     2341 ttttaccgcc atagtatgaa actgccgctg cgctaaaaca atttcaaatc ttcctaaacg
     2401 cccgaaatcc ggtgccttaa gcactttttg atattagctt tgccaaatcg ttattccgtt
     2461 aaggaactac tcattctaat tggtaatttc attcgttctc ttacgctccc tatagtcgaa
     2521 acatctgatg gcaagaaaat agcggtattg caaaggaacg gttatggatc aaaaacgact
     2581 tactcacctg cggcaactgg aggcggaaag catccacatt attcgcgagg tggcggcaga
     2641 attctcaaat ccggtgatgc tctactctat cggtaaagat tccagcgtca tgctgcatct
     2701 ggcgcgcaag gcgttttatc caggtacgct gcctttcccg ttgctgcatg tcgataccgg
     2761 ctggaaattc cgcgagatgt atgagttccg cgatcgtacg gcgaaagcct acggctgcga
     2821 gctgctggtg cataaaaacc cggaaggcgt ggcgatgggg attaatccat tcgtgcacgg
     2881 cagcgcgaaa cataccgata ttatgaaaac tgaaggcctg aaacaggcgc tgaacaaata
     2941 cggttttgat gccgccttcg gtggtgcgcg ccgtgacgaa gagaaatccc gcgctaaaga
     3001 gcgaatctac tctttccgtg accgcttcca tcgctgggat ccgaaaaatc agcgcccgga
     3061 gctgtggcac aactacaacg ggcaaattaa caaaggcgaa agcatccgcg tcttcccgct
     3121 ctctaactgg accgagcagg atatctggca atacatctgg ctggaaaata tcgacattgt
     3181 gccgctgtat ctcgctgcgg aacgtccggt tctggaacgc gacggtatgt tgatgatgat
     3241 tgatgacaac cgtatcgacc tgcaaccggg cgaagtgatc aaaaaacgga tggtgcgttt
     3301 ccgtacgctg ggctgctggc cgctgaccgg tgcggtggag tcaaatgcac aaacactgcc
     3361 ggaaatcatc gaagagatgc tggtttccac caccagtgaa cgtcaggggc gcgtgattga
     3421 ccgcgaccag gcgggatcca tggagctgaa aaaacgtcag gggtattttt aagatgaaca
     3481 ccgtacttgc acaacaaatc gccaatgaag gcggcgtcga agcctggatg attgcgcaac
     3541 aacataaaag cctgctgcgt tttctgacct gtggtagcgt cgatgacggc aaaagtaccc
     3601 tgattggtcg tctgctgcac gatacccgcc aaatctacga agatcagctc tcatcgctgc
     3661 ataacgacag taagcgtcac ggcacccagg gcgaaaagct ggatctggct ctgctggtgg
     3721 acggcctgca agctgagcgc gaacagggca tcactattga tgtggcctac cgctatttct
     3781 ctaccgagaa gcgtaaattt attatcgccg acaccccagg gcacgagcag tacactcgca
     3841 atatggcgac tggcgcatcg acatgtgaac tggcgatctt actgattgat gcccgtaaag
     3901 gcgtgctcga tcaaacccgt cgtcacagtt ttatctccac actgttgggg atcaaacatc
     3961 tggtcgtggc gatcaacaaa atggatctgg tggattacag tgaagagacg ttcacccgta
     4021 ttcgtgaaga ttatctgacc tttgccgggc agctgccgga taatctggat atccgctttg
     4081 tgccgctctc cgcactggaa ggtgacaacg tggcttcgca aagtgaaagt atgccgtggt
     4141 acagcggtct gacactgctc gaagtgctgg aaaccgtgga gatccagcga gtggtggatg
     4201 cccagccaat gcgcttcccg gtgcagtacg ttaaccgccc aaatctcgat tttcgtggct
     4261 acgccggaat gctggcatcc ggtcgcgtgg aagtcgggca acgtgtaaaa gtgctgccct
     4321 ctggtgtgga atcaaacgtc gcgcggatcg tgacttttga tggtgatcgc gaagaagtct
     4381 ttgccggaga agcgatcacc ctggtgctga cggatgagat cgatatcagc cgtggtgatc
     4441 tgctgctggc ggcagacgaa gcgttaccgg cggtgcagag tgcgtcggtg gatgtggtat
     4501 ggatggcgga acagccgctt tccccgggcc agagttatga catcaaaatt gccggtaaga
     4561 agacgcgtgc tcgtgttgat ggcattcgtt atcaggttga tattaataac cttacccaac
     4621 gcgaagttga aaacctgccg ctgaacggca tcggcctggt ggatctcact tttgacgagc
     4681 cactggtgtt agatcgctat cagcaaaatc cggttaccgg tgggctgatt tttatcgatc
     4741 gcctgagcaa tgtgaccgtg ggtgccggta tggtgcacga gccagttagc caggcaacag
     4801 ctgcgtcatc tgaattcagt gcattcgaac tggaattgaa tgccctggtt cgtcgccact
     4861 ttccgcactg gggcgcgcgc gatttgctgg gggataaata atggcgctgc atgacgaaaa
     4921 cgtcgtctgg catagtcatc cggtcactgt gcaacaacgc gaactacacc acggtcatcg
     4981 tggtgtagtg ctgtggttta ccggcctctc cgggtccggt aaatcaacgg tcgccggggc
     5041 gctggaggag gcgttacata aactcagcgt cagtacgtat ctgctggatg gcgacaatgt
     5101 tcgccacgga ttatgcagcg atctcggttt tagcgatgcc gatcgtaaag agaatatccg
     5161 tcgcgtcggt gaagtggcga atttgatggt tgaagccgga ctggtggtgc tgaccgcatt
     5221 tatctcgcca caccgcgccg aacgccagat ggttcgcgaa cgcgtaggag aagggcgctt
     5281 tattgaagtg tttgtcgata cgccgctggc gatttgcgaa gcccgcgatc caaaaggctt
     5341 atataagaaa gcgcgtgccg gtgaactgcg caactttacg ggaatagatt ccgtttacga
     5401 agcgcctgaa tcggcagaaa ttcatctcaa tggtgaacaa ttagtaacaa atttggtaca
     5461 gcaattatta gatctgttga gacagaacga tattatcaga tcctgagaca ccaccgggtt
     5521 tccatgcccg gttcgtcaaa gtaacaggat caaatatgcg taatagccat aacattacac
     5581 taacaaataa cgacagcctt actgaggatg aagaaactac atggtcactg cctggtgccg
     5641 tggtcggttt tatctcctgg ttatttgcgc tggcgatgcc gatgttgatt tatggctcta
     5701 acacgctgtt cttctttctc tacacctggc ctttctttct ggcactgatg cctgtcgcgg
     5761 tagtggtggg gattgcgctg cattcattga tggacggaaa gctacgctac agtattgttt
     5821 tcaccctggt gacagtaggc attatgtttg gctcactgtt tatgtggcta ctgggctaat
     5881 ttgtactttc ttgtctcctc tgttcataat tcaaaccgta actaataatg agattatgtt
     5941 ctgcacgccc tgggtatacg taacaatgga caaatgtggt acatttgccc gcgttgtcgc
     6001 ggtatcccca acagaggatg tagagtcgtc ttcggatgca tgggatgatg atgcagtttt
     6061 tcagggggca ggatgggtaa actaacgctg ctgttgctgg ctattctggt ctggctacag
     6121 tattcgctgt ggttcggtaa gaacggtata catgactata cccgcgtcaa tgatgatgtg
     6181 gcggcactgc aagctacaaa cgcgaaactt aaagcgcgaa acgatcaact ttttgccgaa
     6241 attgacgatc tcaatggcgg ccaggaggcg ctcgaagagc gtgcgcgtaa tgaactcagc
     6301 atgaccaggc cgggcgaaac tttttatcgt ctggtgcctg acgcgtcgaa gcgcgcacag
     6361 tctgcggggc aaaacaatcg ataaatcagc ccgggaatta acatggcaac cactcatttg
     6421 gatgtttgcg ccgtggttcc ggcggccgga tttggccgtc gaatgcaaac ggaatgtcct
     6481 aagcaatatc tctcaatcgg taatcaaacc attcttgaac actcggtgca tgcgctgctg
     6541 gcgcatcccc gggtgacacg tgtcgtcatt gccataagtc ctggcgatag ccgttttgca
     6601 caacttcctc tggcgaatca tccgcaaatc accgttgtag atggcggtga tgagcgtgcc
     6661 gattccgtgc tggcaggtct gaaagccgct ggcgatgtgc agtgggtatt ggtgcatgac
     6721 gccgctcgtc cttgtttgca tcaggatgac ctcgcgcgat tgttggcgtt gagcgaaacc
     6781 agccgcacgg gagggatcct cgccgcacca gtgcgcgata ctatgaaacg tgccgaaccg
     6841 ggcaaaaatg ccattgctca taccgttgat cgcaacggct tatggcacgc gctgacgccg
     6901 caatttttcc ctcgtgagct gttacatgac tgtctgacgc gcgctctaaa tgaaggcgct
     6961 accattaccg acgaagcctc ggcgctggaa tattgcggat ttcatccgca gttggtcgaa
     7021 ggccgcgcgg ataacattaa agtcacgcgc ccggaagatt tggcactggc cgagttttac
     7081 ctcacccgaa ccatccatca ggagaataca taatgcgaat tggacacggt tttgacgtac
     7141 acgcctttgg cggtgaaggc ccaattatca ttggtggcgt acgcattcct tacgaaaaag
     7201 gattgctggc gcattctgat ggcgacgtgg cgcttcatgc gttgaccgat gcattgcttg
     7261 gcgcggcggc gctgggggat atcggcaaac tgttcccgga taccgatccg gcatttaaag
     7321 gtgccgacag ccgcgagctg ctacgcgaag cctggcgtcg tattcaggcg aagggttata
     7381 cccttggcaa cgtcgatgtc actatcatcg ctcaggcacc gaagatgttg ccgcacattc
     7441 cacaaatgcg cgtgtttatt gccgaagatc tcggctgcca tatggatgat gttaacgtga
     7501 aagccactac tacggaaaaa cttggattta ccggacgtgg ggaagggatt gcctgtgaag
     7561 cggtggcgct actcattaag gcaacaaaat gattgagttt gataatctca cttacctcca
     7621 cggtaaaccg caaggcaccg ggctgctgaa agccaatccg gaagactttg tggtggtgga
     7681 agatttgggc tttgagcctg atggtgaagg tgagcatatt ctggttagaa tcctcaaaaa
     7741 cggctgcaat acccgttttg tggcggatgc actggcgaaa ttcctgaaaa ttcattcccg
     7801 tgaagtcagc ttcgctgggc aaaaagacaa acatgctgta acggaacagt ggttatgtgc
     7861 tcgcgtgccg ggcaaggaaa tgcccgatct gagcgccttt caactggaag gctgccaggt
     7921 gctggagtat gcgcggcaca agcgcaagct gcgtttaggg gcgctgaaag gtaacgcctt
     7981 taccctggtt ctgcgcgaag tgagcaatcg cgatgacgtt gaacaacgtc tgatcgatat
     8041 ttgcgtaaaa ggtgtaccga actacttcgg tgcccaacgt tttgggattg gcggtagcaa
     8101 cttgcagggc gcgctgcgct gggcgcaaac caatactccg gtgcgcgatc gcaataaacg
     8161 gagtttttgg ttgtcggcag cccgcagtgc gttgtttaat cagattgttg ctgagcgcct
     8221 caaaaaagca gacgttaatc aagttgttga cggcgatgcg ctacaattag ccggacgtgg
     8281 tagctggttt gtagcaacca ccgaagaact ggcggaatta cagcgtcgcg tcaacgataa
     8341 agagctgatg ataaccgccg cattgccggg cagtggcgaa tggggaactc agcgtgaagc
     8401 gctggcattc gaacaagcag ctgtcgccgc agaaactgaa ttacaagctt tactggtgcg
     8461 cgaaaaagtt gaagccgcgc gcagagcgat gctgctgtat ccgcaacaat taagctggaa
     8521 ctggtgggat gacgtcaccg tagagatccg tttctggctt ccggcgggta gttttgcaac
     8581 cagcgttgtc agggaactta tcaacacaac aggtgattat gcgcatattg ctgagtaatg
     8641 atgacggggt acatgcaccc ggtatacaaa cgctggcgaa agccttgcgt gagtttgctg
     8701 acgttcaggt ggtcgccccc gatcgtaacc gcagcggcgc ttcaaattct ctgacactgg
     8761 aatcctccct gcgcacgttt acctttgaaa atggtgatat tgctgtgcaa atgggaaccc
     8821 cgaccgattg tgtctatctt ggcgtgaatg ctctgatgcg tccgcgcccg gacattgttg
     8881 tgtccggaat taacgccggg ccgaatctgg gggatgatgt tatttattcc ggtacggtag
     8941 ccgccgcgat ggaaggccgt catttaggtt ttccggcgct ggccgtctcg cttgacgggc
     9001 ataaacatta cgacactgcc gcggcggtaa cctgttcaat tttgcgcgca ctgtgtaaag
     9061 agccgctgcg caccggacgt attcttaata ttaacgttca ggatttaccc ttggatcaaa
     9121 tcaaaggtat tcgcgtgacg cgctgcggta cacgacatcc ggcagatcag gtgatcccgc
     9181 agcaagatcc gcgcggcaat acgctgtact ggattggccc gccgggcggt aaatgtgatg
     9241 ccggtccggg gaccgatttt gctgcggtag atgagggcta tgtctccatc acgccgctgc
     9301 atgtggattt aactgcgcat agcgcgcaag atgtggtttc agactggtta aacagcgtgg
     9361 gagttggcac gcaatggtaa gcagacgcgt acaagcactt ctggatcaat tacgtgcgca
     9421 aggtattcag gatgagcagg tgctgaatgc acttgccgcc gtgccgcgtg aaaaattcgt
     9481 tgatgaagcg tttgaacaaa aagcctggga caatatcgcc ttgccgatag gtcaggggca
     9541 gacaatttcg cagccgtata tggtggcgcg aatgaccgaa ttactcgagc tgacgccgca
     9601 gtcgcgggtg ctggaaattg gcactggttc gggatatcaa acggcaatcc tggcgcatct
     9661 tgtccagcat gtttgctcgg ttgaacggat taaaggcttg cagtggcagg cacgtcgccg
     9721 cctgaaaaat cttgatttac ataatgtttc aacccgtcat ggtgatggat ggcaaggttg
     9781 gcaggcacgt gcgccgtttg acgctatcat tgttacggcg gcaccgccgg aaattccaac
     9841 tgcgctaatg acgcagctgg acgaaggcgg gattctcgtc ttacccgtag gggaggagca
     9901 ccagtatttg aaacgggtgc gtcgtcgggg aggcgaattt attatcgata ccgtggaggc
     9961 cgtgcgcttt gtccctttag tgaagggtga gctggcttaa aacgtgagga aatacctgga
    10021 tttttcctgg ttattttgcc gtaggtcagc gtatcgtgaa catcttttcc agtgttcagt
    10081 agggtgcctt gcacggtaat tatgtcactg gttattaacc aatttttcct gggggataaa
    10141 tgagcgcggg aagcccaaaa ttcaccgttc gccgcattgc ggctttgtca ctggtttcgc
    10201 tatggctggc aggctgttct gacacttcaa atccaccggc accggtcagc tccgttaatg
    10261 gcaatgcgcc tgcaaatact aattctggta tgttgattac gccgccgccg aaaatgggga
    10321 cgatgtctac agcgcagcaa ccgcaaattc agccggtaca gcagccacaa attcaggcta
    10381 ctcaacaacc gcaaatccag ccagtgcagc cagtagctca gcagccggta cagatggaaa
    10441 acggacgcat cgtctataac cgtcagtatg ggaacattcc gaaaggcagt tatagcggca
    10501 gtacctatac cgtgaaaaaa ggcgacacac ttttctatat cgcctggatt actggcaacg
    10561 atttccgtga ccttgctcag cgcaacaata ttcaggcacc atacgcgctg aacgttggtc
    10621 agaccttgca ggtgggtaat gcttccggta cgccaatcac tggcggaaat gccattaccc
    10681 aggccgacgc agcagagcaa ggagttgtga tcaagcctgc acaaaattcc accgttgctg
    10741 ttgcgtcgca accgacaatt acgtattctg agtcttcggg tgaacagagt gctaacaaaa
    10801 tgttgccgaa caacaagcca actgcgacca cggtcacagc gcctgtaacg gtaccaacag
    10861 caagcacaac cgagccgact gtcagcagta catcaaccag tacgcctatc tccacctggc
    10921 gctggccgac tgagggcaaa gtgatcgaaa cctttggcgc ttctgagggg ggcaacaagg
    10981 ggattgatat cgcaggcagc aaaggacagg caattatcgc gaccgcagat ggccgcgttg
    11041 tttatgctgg taacgcgctg cgcggctacg gtaatctgat tatcatcaaa cataatgatg
    11101 attacctgag tgcctacgcc cataacgaca caatgctggt ccgggaacaa caagaagtga
    11161 aggcggggca aaaaatagca accatgggta gcaccggaac cagttcaaca cgcttgcatt
    11221 ttgaaattcg ttacaagggg aaatccgtaa acccgctgcg ttatttgccg cagcgataaa
    11281 tcggcggaac caggcttttg cttgaatgtt ccgtcaaggg atcacgggta ggagccacct
    11341 tatgagtcag aatacgctga aagttcatga tttaaatgaa gatgcggaat ttgatgagaa
    11401 cggagttgag gtttttgacg aaaaggcctt agtagaagag gaacccagtg ataacgattt
    11461 ggccgaagag gaactgttat cgcagggagc cacacagcgt gtgttggacg cgactcagct
    11521 ttaccttggt gagattggtt attcaccact gttaacggcc gaagaagaag tttattttgc
    11581 gcgtcgcgca ctgcgtggag atgtcgcctc tcgccgccgg atgatcgaga gtaacttgcg
    11641 tctggtggta aaaattgccc gccgttatgg caatcgtggt ctggcgttgc tggaccttat
    11701 cgaagagggc aacttggggc tgatccgcgc ggtagagaag tttgacccgg aacgtggttt
    11761 ccgcttctca acatacgcaa cctggtggat tcgccagacg attgaacggg cgattatgaa
    11821 ccaaacccgt actattcgtt tgccgattca catcgtaaag gagctgaacg tttacctgcg
    11881 aaccgcacgt gagttgtccc ataagctgga ccatgaacca agtgcggaag agatcgcaga
    11941 gcaactggat aagccagttg atgacgtcag ccgtatgctt cgtcttaacg agcgcattac
    12001 ctcggtagac accccgctgg gtggtgattc cgaaaaagcg ttgctggaca tcctggccga
    12061 tgaaaaagag aacggtccgg aagataccac gcaagatgac gatatgaagc agagcatcgt
    12121 caaatggctg ttcgagctga acgccaaaca gcgtgaagta ctggcacgtc gattcggttt
    12181 gctggggtat gaagcggcaa cactggaaga tgtaggtcgt gaaattggcc tcacccgtga
    12241 acgtgttcgc cagattcagg ttgaaggcct gcgccgtttg cgcgaaatcc tgcaaacgca
    12301 ggggctgaat atcgaagcgc tgttccgcaa gtaagtaagc atctgtcaga aaggccagtc
    12361 tcaagcgagg ctggcctttt tcttttgttt gggaagcttg tgtttttgtc gcctggataa
    12421 gacacgtcag cgtcctaccc ggcaatcagc attctatgtg ctcatcatct tgcgcaccaa
    12481 ctgcataaac agctcctgct cttctccact caaacgcccc aaaaattcct catccacgct
    12541 gtcaccaata ggtatcgccg cagcaagtag cttttctcct tcggcagtca gccagacaaa
    12601 gcgtcgccgc ttgtcagcga cgtcatgttc acgtctgact aagccacgat tttccattct
    12661 tgccaacatt tctgctagcg ttgctttggt gctgactgct gcttctatca gtgccacctg
    12721 ttcaatacca ggtttatcag caatggcacg catcaccgcg tactgtggtt tggtcaggtc
    12781 aggtaactcg tgctgccagc gagccgtgtg ctgctgaaaa agctgtcgta actgatggaa
    12841 cgctttattt cgtaacgcca tgtaaactcc gggctaattc tctgtcgcta tcatagcggt
    12901 tttttagtga cgaagaagat gttgttactt ttcaatcctt gccgctgtgg aaatgaagga
    12961 gtatgttaat aataacgttc gtatacgaat aattaagaga ataagcaatg aaactgatcg
    13021 tcgggatgac aggggctacc ggtgcgcctc ttggtgtggc attactgcaa gcgctgcgtg
    13081 agatgccgaa tgtcgagact catctggtga tgtcgaagtg ggcgaaaacc accattgaac
    13141 tggaaacgcc ttacagcgct cgcgatgttg ctgccctcgc agacttcagc cataacccgg
    13201 cggatcaggc ggcgatcatc tcatccggtt cttttcgtac cgacggcatg atcgttattc
    13261 cgtgcagtat gaaaacgctc gccggtatcc gcgctggtta cgccgatggc ctggtagggc
    13321 gcgcggcgga cgtcgtgctc aaagaaggcc gcaaactggt gctggtgccg cgtgaaatgc
    13381 cgcttagcac catccatctc gaaaatatgc tcgcactttc acgcatgggc gtggcgatgg
    13441 tgccgccgat gcctgccttt tataaccatc ccgaaacggt agatgacatt gtccaccatg
    13501 tggtagcccg cgtgctggat caatttggcc tcgaacatcc ccacgccagg cgctggcaag
    13561 gattaccgca ggcccggaat ttttctcagg agaatgaata atggcatttg atgatttacg
    13621 cagcttttta caggcgcttg atgaccacgg ccagttactg aaaatcagcg aagaagtgaa
    13681 cgctgagccg gatctggcag cagcagctaa cgccaccggg cgtatcggcg acggcgcgcc
    13741 cgcgctgtgg tttgataata ttcgcggctt taccgatgcc cgcgtggcga tgaataccat
    13801 cggttcctgg cagaaccacg cgatttccct tggcctgcca ccaaacaccc cggtcaaaaa
    13861 gcagattgat gagtttatcc gccgctggga taacttcccg attgccccgg agcgccgcgc
    13921 caatccagcc tgggcgcaga acaccgtcga tggcgaagag atcaacctgt tcgatattct
    13981 gccgctgttt cgcctgaatg atggcgatgg cggtttctat ctcgacaaag cgtgcgtggt
    14041 ttcccgcgat ccgctcgacc cggataactt cggcaagcag aacgtcggca tctaccgcat
    14101 ggaagtgaag ggcaagcgta agctcggcct gcaaccggtg ccgatgcacg atatcgccct
    14161 gcatctgcat aaagcagaag agcgcggtga agatctgccg attgcgatca cgctcggtaa
    14221 cgatccgatc atcacgctga tgggggccac gccgctgaaa tacgatcagt ctgagtatga
    14281 aatggcaggc gcgctgcgtg aaagcccgta cccgatcgcc accgctccgc tgaccggttt
    14341 tgatgtgccg tggggttcag aagtgatcct cgaaggggtt atcgaaagcc gtaaacgcga
    14401 aatcgaaggg ccgttcggtg agtttaccgg gcactactcc ggtgggcgca acatgaccgt
    14461 ggtgcgcatc gataaagtct cttatcgcag caaaccgatt ttcgaatcac tctatctcgg
    14521 tatgccgtgg accgaaatcg actacctatg actaggtaat ttattcaaga taaccgggaa
    14581 gagattaaaa aagacgggaa tagattggaa tcaacaagtt aggaactcaa ggttttcaag
    14641 ataaagcgag tccaaaaatg ttaaacggtt cgatatcaat aaaacattca agataactca
    14701 caggttctta caatagcctt taaagaccgt tcaaaaacga ttcaggtgtt tattatagcg
    14761 ctgatgggtg caagcgccaa aaccaatgta attttagcac agctaggcac ttgcactggg
    14821 ttatgcgctg gctgctttct gaggtagatt taagtgatcg cccttagctt cggacaacgc
    14881 gtaaatctct ctaacctttt cgtctggcaa attaccaagg cttaacgcta tggctctacg
    14941 aactccaact agagcgtgca actcttggat gtgctcatcc agaagaatga gtaattgctc
    15001 cgctcctttt ctcccaagaa catcagctac ttgttgtttt tcttgtggtt taagaaatga
    15061 caagaacatt agaatttgag catcaacatt atccgcattc tcttttttgg gttctttgga
    15121 atcttttagt tcaacatcac cctctcccgt catgagccat tccaaggtaa cattttcgct
    15181 ttctgctatc ttttttaact gatgtgggcg tggtatcggt gcgtaatccc gcctgttgta
    15241 ataatttttt aaagtgcctt cattgatccc ccatgcccga gcagccgcat ttcttgaagg
    15301 gtatcgttct actaacctaa agattctttc gataatcggt tcttttccat cggcaggaaa
    15361 agaaccttgg ggtgcttttc tcattcttgg ttcctttcta tctaattcat tgataaataa
    15421 taaaattaaa aaataaacca taaaaacacc aaagaaccct ttacagggaa tctttggtat
    15481 ctcaatcttt atccatacgg atatccgacc ggatatccgc gcgggtaatt ttgtaggtta
    15541 aaggaaagat gagacaaaga gaagatagtg accgcgattg gccttcgctg aagattcagc
    15601 tggcgctcaa agataacggg tggtcttcct tccgggagct ttcactccat gctggttatg
    15661 ctcaggattc actgaaagcg gtagttcgca caccaaacaa aaaatacgaa cgcgtaattt
    15721 ctgacgctat aggtgtaccg ccagaaatca tctggcccag ccggtatcgt aacgatggat
    15781 ttatcaggaa ggcgtcgtga tatgcagtcg tacgttaccg taaatgacct gctcggcgta
    15841 ccaggaatgc cagccaccac aaaaggcatc cgacaggcgt tacagcgttt ttctcgtgat
    15901 cttggtgacg ttagtcgccg tcgggaaggc acaaaagcca ttgagtacca cattgattgt
    15961 ctgcctgaaa taacacgaaa ggcactgcgt gaacgctatg tggaacagct ggtggctaca
    16021 gagaacaatg tttctgaagt gaaagctgtt accagaaaaa cacgcaatcc tgacgctgtc
    16081 caggcaatcg aagcatatcg cggttctcca caactgatgg aagaacgcct gaatgcgctg
    16141 accgaaaacc agcgctgggt atctgaagca agagctgcgc tggtggtgga agtgctgaag
    16201 ctggaaagcg ccggtaaccc cgggcgactg aaagccatta actttcttgt tgaaaaagcc
    16261 cgtaaaggtg agctgccgga gcgcctgcaa caggccgcag ttaacgccaa tgcaaaacgt
    16321 ggcgctaatc gtactatcag ccgcgatcca ctttaccagt gggttctgaa atacaaccag
    16381 tcgcagaacg ccgccgaacg gctgctgtta ctggcaccgg gaaaacgcga cgaaataaaa
    16441 ccggaagaaa tcagctggct gcctgaattt ctggcgcagt atcgccaggt gaacggcaga
    16501 cccatgtcag aagcctacga ggattttgtc gcagaatggc agcgtcgcca tgcggacgaa
    16561 ccgtacatgc tggaggtgat gccttcctat gatgttgtcc ggtatgccat gaaaaaactg
    16621 ccggaagtgg tgaaacaaaa agggcgcgtt accggcagtg aataccgtca gcttgaagga
    16681 ttcacccgcc gcgactggac agctatgccg gtgaattatg tctggattgg tgacggtcac
    16741 ggtatgaagc tgaaatgtgc gcatccgatc cacgggcgtc cattctcacc ggaagtcacg
    16801 tttgtgatcg atggtggaac acggtttgtt gttggctgga gtcttgacct tgccgaaaac
    16861 gtgttcgccg ttgccggtgc gatacagcac ggcattcgca accacggaaa accgttcctg
    16921 tattactcgg ataacggttc cggtgaaacg gctgacatgc tggacaaaga ggtcgtgggt
    16981 attctgccac gcctggggat taaacaccct accggtattg ccggtaatcc acagggacgc
    17041 ggcattattg aacgcctgaa ccggacactg ccgatgcgta tagccaggag gtatcgcacc
    17101 tattttggca agggtgctga ccgggaatcg ttgcgtgtac ttaaccgtga tctgcgttca
    17161 gcgtttaacg ccctgcaaca ggacaagcct ctgaacgacc gccagaaagc ggcaatgcgc
    17221 gagctgccat cgtgggcgga gcttattgag gctattcgtg aaggcgtgga atggtacaac
    17281 aaccgcccgc attctgagct gccgatgaaa ccgaacggac ggcattacag cccgacagag
    17341 ttcagaaaaa aacgccaggc agaagaagac accgaaattg agtggctgag tgatcttgag
    17401 ctgcgcgaca tgttccgccc aatggtggaa cgaccggtaa gacgctgcga aattcagtgg
    17461 ctgaacaata tttactacgc gccggagttg cgtgacgaac acggtcgcaa agtgcttatc
    17521 agttatgaca ttcatgacgc cgagcgaatt acggtacgtc gcaaggacgg cagctttatc
    17581 tgtgaggcga tatggaacgg caataaacgc gcagcctttg ctgtcagtgc tgaatatcac
    17641 aaacaacagc agcgtattaa aggaatgcgc aaacgcgctg aggaaaaaat tcgtgatgct
    17701 gaagatgaag gtattcagat tctggaacac aaacaggctg aaccctggct gagtaatgtt
    17761 tatcgcccgg ttggtaatgt ggtggcagtt cagcaaccgg aatatgaaga agagcatgac
    17821 gaagaatttg aacgcgattt ccggctgggg atgcaaaaac tttttgccat gcaggaagaa
    17881 gatgatccgc tggcctgaat aaaaaaacag tccgcgggaa cggactgtta aataaaggaa
    17941 acaaagctga tttgaaataa ccgaggaaat actatgactg atattgatga tgt
//
LOCUS       DAECNO010000083        17633 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.083, whole genome shotgun sequence.
ACCESSION   DAECNO010000083 DAECNO010000000
VERSION     DAECNO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.083"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            322..1005
                     /locus_tag="KKO08_002926"
     CDS             322..1005
                     /locus_tag="KKO08_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBD6319097.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1049..5414
                     /locus_tag="KKO08_002927"
                     /pseudo
     CDS             1049..5414
                     /locus_tag="KKO08_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5782..7437
                     /gene="lldP"
                     /locus_tag="KKO08_002928"
     CDS             5782..7437
                     /gene="lldP"
                     /locus_tag="KKO08_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBD6319098.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7437..8213
                     /gene="lldR"
                     /locus_tag="KKO08_002929"
     CDS             7437..8213
                     /gene="lldR"
                     /locus_tag="KKO08_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBD6319099.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8210..9400
                     /gene="lldD"
                     /locus_tag="KKO08_002930"
     CDS             8210..9400
                     /gene="lldD"
                     /locus_tag="KKO08_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBD6319100.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9596..10069
                     /gene="trmL"
                     /locus_tag="KKO08_002931"
     CDS             9596..10069
                     /gene="trmL"
                     /locus_tag="KKO08_002931"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBD6319101.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10122..10943)
                     /gene="cysE"
                     /locus_tag="KKO08_002932"
     CDS             complement(10122..10943)
                     /gene="cysE"
                     /locus_tag="KKO08_002932"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBD6319102.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11023..12042)
                     /gene="gpsA"
                     /locus_tag="KKO08_002933"
     CDS             complement(11023..12042)
                     /gene="gpsA"
                     /locus_tag="KKO08_002933"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBD6319103.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12042..12509)
                     /gene="secB"
                     /locus_tag="KKO08_002934"
     CDS             complement(12042..12509)
                     /gene="secB"
                     /locus_tag="KKO08_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBD6319104.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12572..12823)
                     /gene="grxC"
                     /locus_tag="KKO08_002935"
     CDS             complement(12572..12823)
                     /gene="grxC"
                     /locus_tag="KKO08_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBD6319105.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12965..13396)
                     /locus_tag="KKO08_002936"
     CDS             complement(12965..13396)
                     /locus_tag="KKO08_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBD6319106.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13641..15185
                     /gene="gpmM"
                     /locus_tag="KKO08_002937"
                     /gene_synonym="pgmI"
     CDS             13641..15185
                     /gene="gpmM"
                     /locus_tag="KKO08_002937"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBD6319107.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15195..16478
                     /gene="envC"
                     /locus_tag="KKO08_002938"
     CDS             15195..16478
                     /gene="envC"
                     /locus_tag="KKO08_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBD6319108.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16482..17441
                     /locus_tag="KKO08_002939"
     CDS             16482..17441
                     /locus_tag="KKO08_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBD6319109.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17428..17633)
                     /locus_tag="KKO08_002940"
                     /pseudo
     CDS             complement(17428..17633)
                     /locus_tag="KKO08_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4428 a         4562 c         4584 g         4059 t
ORIGIN      
        1 cgtctctttt tttcggattt aattgtgaaa ttttaatcat catattcaca aaatcaatta
       61 tctgtgaaat ttttcccaga catggtttct ttcatatttg acatttatta ataatcatca
      121 ttagaatcca cgaaaaataa tatattcata caaacacaga catcttttga aacgaatgat
      181 acatttttat gatgaatgct taaaacaata tttaatcgac acacatcact gtcattaatg
      241 taaagcaaca tagctcacca ataatacaat ggacaactct accggcattg atcgctggtc
      301 ggaggaaata aggattttta catgaacttg aagaagattt ttttcagtgc tgtcactgtt
      361 tcagttctct gcgcattaac gggatgtgac tacattgaag aaggcaagcc agaaagtagt
      421 ttactgaaac aacaagaaga acataataat aaaattgtgc tacttgaaaa acaacaagca
      481 cagctaaaga gtcaactcga aactattcaa aaacaacaaa ctggcataat aaacagtaca
      541 aagactttaa ctcacgtaat taaatccgtt aaagaccaac aaaatacttt tatttttacc
      601 gagtttaacc cagcaaaaac gaagtatttc atcctgaaca acggttcggt ggctttagcg
      661 ggtcgagtgt tatccattga cgccacagag aatggtagtg ttattcatat ctcactggtc
      721 aacttattaa gtacacctat ctcaaacatt ggttttaatg cgacatgggg tggtgaaaaa
      781 cctgtcgatg ccaaagagtt tgcccggtgg caacaattac ttttcaacac atcaatgaaa
      841 tccacattga aattattacc aggtcaatgg caagacatta atttgaccct gaagggtgta
      901 tcgcccaata acctgggata tctgaagtta gccatcaaca tggaaaatat tcagttcgac
      961 aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa aataaatgca gttagtacgc
     1021 ttataacaag gaatagcgag catcattaat gaacaaaata tttaaagtta tctggaaccc
     1081 tgcgacaggg aattatactg ttaccagcga aacggcaaaa agccgtggca agaaatctgg
     1141 gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga atgttgtcgt cgtttggggc
     1201 attggcgaat gccgggaatg acaacggtca gggtgttgat tacggtagtg gatcagctgg
     1261 cgacggctgg gttgctatag gcaaaggggc gaaagcaaat acttttatga acaccagtgg
     1321 ttccagtact gctgtgggtt atgacgctat agctgaaggc caatatagct ctgccatcgg
     1381 gtcaaaaacc catgcgattg gtggtgcatc aatggccttt ggggttagtg caatatcaga
     1441 aggcgataga agtatagcac tgggtgcctc ttcgtattca ttgggccaat actcaatggc
     1501 cctcggccgt tattcaaaag cattgggtaa attgtctatt gctatggggg actcttccaa
     1561 agcggaagga gcaaacgcca ttgccctggg gcgtagcagt gtagctagcg gtactgacag
     1621 cctcgcattt ggcagacaat cacttgccag cgcagcgaac gctattgcga taggtgctga
     1681 gaccgaagcc gctgaaaatg caactgctat tggcaataat gcgaaggcaa aagggactaa
     1741 tagcatggca atggggttcg gaagccttgc cgataaagtc aatactatcg cattaggaaa
     1801 tagcagccag gctctggcag ataatgcaat cgccataggc cagggcaaca aagctgatgg
     1861 cgtggatgcc atcgctctgg gtaatggtag ccagtcgaga ggcttaaaca ccattgcctt
     1921 aggcacagcc agtaatgcaa ctggtgataa gagtcttgcg cttggtagta atagcagtgc
     1981 caacggtatt aactctgtcg cgctgggcgc agattccatt gcggatttag acaataccgt
     2041 ctctgtcggc aatagttcat taaaacgcaa gatcgttaat gtgaaaaatg gcgcgatcaa
     2101 gtctgacagt tacgatgcca ttaatggttc acagctttat gccattagcg actcggtagc
     2161 aaaaaaggct tggaggaggg gctgcagtag atgttgatga cggtactgtt acagcaccaa
     2221 cctacaattt aaaaaatggt agcaaaaata acgtaggggc tgcgctcgct gtacttgatg
     2281 aaaacaccct gcaatgggac caaaccaaag gcaaatacag cgctgctcat ggtactagta
     2341 gcccaactgc cagcgtaatc accgatgttg cggatggcac gatttcagcc tccagtaagg
     2401 atgcggttaa cggttcccaa ctgaaagcta ccaatgacga tgtcgaagcc aacaccacca
     2461 atatcaccaa cctgacggat tccgttggtg accttcaggc tgatgccctg ctctggaacg
     2521 aaactaaaaa ggcattcagt gcagctcacg gccaggatac caccagcaaa atcaccaacg
     2581 ttaaagatgc cgacctgacg gctgacagca ctgatgctgt taacggctct cagctgaaaa
     2641 ccaccaacga tgctgtggcg acgaatacca ccaatatcgc caataacact tccaatattg
     2701 ccactaacac caccaacatc tctaacctga ctgagacggt gactaatctt ggtgaggatg
     2761 cgctgaaatg ggataaggac aatggtgtat tcacggcagc tcatggcacc gagaccacca
     2821 gcaaaataac caacgttaaa gatggcgacc tgacgactgg cagcaccgat gccgttaacg
     2881 gctctcagct gaaaaccacc aacgatgccg tggcgacgaa taccaccaat atcgccacta
     2941 acaccaccaa catctctaat ctgactgaga cggtgactaa tcttggtgag gatgcgctga
     3001 aatgggataa ggacaatggt gtcttcactg cagctcatgg caacaatacc gccagcaaaa
     3061 tcaccaatat cctggacggc acagtcactg caaccagttc cgatgccatt aacggtagcc
     3121 agctttatga cttaagcagc aatatcgcca cctacttcgg cggcaatgct tctgtgaata
     3181 ctgacggtgt gtttaccggt ccaacctaca aaatcggtga aacaaattat tataacgtcg
     3241 gcgatgcact ggctgcgatt aactcctcat ttagcacgtc tctcggcgat gctctgcttt
     3301 gggatgccac cgcaggtaaa ttcagtgcca aacacggtac taatggtgac gcaagcgtga
     3361 tcactgatgt cgcagatggt gaaatttcag actccagttc tgacgcagta aacggctcac
     3421 aactccacgg cgtgagcagt tatgttgttg atgcgctggg gggtggtgcc gaagtcaatg
     3481 cagacggcac catcaccgca ccgacttaca ccattgcgaa cgccgagtac aacaacgtcg
     3541 gtgacgccct ggatgcgctt gatgataacg ccctgctgtg ggataagact gccaatggcg
     3601 gtgctggagc ctacaatgcc agccatgacg gtaaagccag catcatcact aatgtcgcta
     3661 atggcagtat tagtgaggac agtaccgatg cagtgaacgg ttctcagttg aatgcgacga
     3721 atatgatgat tgagcagaac acccaaatta tcaatcagct cgctggtaac accgacgcaa
     3781 cctatatcga agaaaatggc gctggtatca actacgttcg tactaacgac aacgatttag
     3841 cctttaacga tgcaagcgcc tctggtgtcg gcgctacagc tgtaggttat aacgctgtcg
     3901 cgtctggtgc cagcagcgta gccattggtc agaacagcag cagcaccgtt gataccggta
     3961 ttgcgctggg tagcagctcc gtttccagcc gtgtgattgc caaaggttct cgtgacacta
     4021 gcgtaacgga aaatggcgtg gtgattggtt atgacaccac tgacggcgaa ctgctaggtg
     4081 cattgtcaat tggtgatgac ggtaaatacc gccaaatcat caacgtagct gatggttcag
     4141 aagcccatga cgccgttacg gttcgccagt tgcagaacgc tattggagcg gtcgccacta
     4201 cgccaaccaa gtactttcac gccaactcaa cggcagaaga ctcactggcc gttggtgaag
     4261 actcgctggc aatgggtgcg aaaactgtcg ttaatggtaa tgcaggtatt ggtatcggtt
     4321 tgaacactct ggttctggct gatgcgatca acggcattgc tatcggcagt aacgcacgcg
     4381 caaaccatgc aaacagtatc gcaatgggta acggttctca gaccacccgt ggtgcacaga
     4441 ctggctacac cgcctacaac atggacgcac cgcagaactc tgtaggtgag ttttctgtcg
     4501 gtagtgaaga cggtcaacgt cagatcacaa acgtcgcagc tggttcagcg gataccgatg
     4561 cggttaacgt gggtcagttg aaagtcactg atgagcgcgt agcgcaaaat acccagagca
     4621 ttactaacct gaacaatcag gtcactaatc tggatactcg cgttactaat atcgaaaacg
     4681 gtattggcga cattgtcacc accggtagca ccaagtactt caagaccaac accgatggcg
     4741 tagatgccaa cgcccagggt aaagatagcg ttgctattgg ttctggttcc attgctgccg
     4801 ctgacaacag cgtcgcactg ggtaccggtt ccgttgcaga ggaagaaaat acaatctctg
     4861 taggttcttc tactaaccaa cgccgtatca ccaacgtagc tgccagtgtt aatgccaccg
     4921 atgcggttaa cgtgtcgcag ctgaaatctt ctgaagcagg cggagtgcgc tacgacacca
     4981 aagctgatgg ttctatcgac tatagcaata tcaccctcgg tggtggcaac ggcagtacga
     5041 ctcgtatcag caacgtctcc gctggcgtca acaacaacga cgcggtgaac tacgcgcagt
     5101 tgaagcaaag cgcgcaggaa acgaagcaat acaccgatca gcggatggtt gagatggata
     5161 acaaactgtc taaaactgaa agcaagttga gcggtggtat cgcttctgca atggcaatga
     5221 ccggtctgcc gcaggcttat acaccgggtg ccagcatggc ttctattggt ggcggtactt
     5281 acaacggtga atcggcagtt gctttaggtg tatcgatggt gagcgccaat ggtcgttggg
     5341 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
     5401 gtattcagtg gtaatgatcc attaagttag tgtgactaag ggcaaacgcc aaattgcctg
     5461 atgcgttacg cgcatcaggc aagataaggc gctccgggtc aacaatctaa gggcaattct
     5521 ctgatgagga ttgccctttt ctttaccaga catctccccc cacaagaatt ggccctacca
     5581 attcttcgct tatctgacct ctggttcaca attccccaat taaaactcac atcaatattg
     5641 ccaatacata acatttagtt aaccattcat tgtcattatc cctacacaac acaattggca
     5701 gtgccacttt tacacaacgt gtgacaagga gatgagcaac agactcatta cacgatgtgc
     5761 gtggactcta ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat
     5821 atctggcttt ccagtctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt
     5881 aagctcaaac tgaaaggata cgtcgccgcc tcatggacgg tagcaatcgc ccttgccgtg
     5941 gctttgctgt tctataaaat gccggtcgct aacgcgctgg cctcagtggt ttatggcttc
     6001 ttctatggtt tgtggcccat cgcgtggatc attattgcag cagtgttcgt ctataagatc
     6061 tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta ttctttcgat aacccctgac
     6121 cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg ctttccttga aggagccgca
     6181 ggctttggcg caccggtagc aattaccgcc gcattgctgg tcggcctggg ttttaaaccg
     6241 ctgtacgccg ccggactgtg cctgattgtt aacaccgcgc cagtggcatt tggcgcgatg
     6301 ggcattccaa tcctggttgc cggacaagta acaggcatcg acagctttga gattggccag
     6361 atggtggggc gtcagctacc gtttatgacc attatcgtgc tgttctggat catggcgatt
     6421 atggacggct ggcgcggtat caaagagacg tggcctgcgg tcgtggttgc aggcggctcg
     6481 tttgccatcg ctcagtacct tagctctaac ttcattgggc cggagctgcc ggacattatc
     6541 tcttcgctgg tatcactgct ctgcctgacg ctgttcctca aacgctggca accagttcgc
     6601 gtattccgct tcggtgactt gggggcgtca caggttgata tgacgctggc ccacaccggt
     6661 tacaccgcgg gtcaggtgtt acgtgcctgg acaccgttcc tgttcctgac cgctaccgta
     6721 acactgtgga gcatcccgcc gtttaaagcc ctgttcgcat cgggtggtgc gctgtatgag
     6781 tgggtgatca acattccggt gccgtacctc aataaactgg ttgcccgtat gccaccagtg
     6841 gtcagcgagg ctacagccta tgccgccgtg tttaagtttg actggttctc tgccaccggc
     6901 accgccattc tgtttgctgc actgctctcg attgtctggc tgaagatgaa accgtctgac
     6961 gctatcagca ccttcggcag cacgctgaaa gaactggctc tacccatcta ctccatcggc
     7021 atggtgctgg cgttcgcctt tatctcgaac tattccggac tgtcatcaac actggcactg
     7081 gcactggcgc acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggctgggg
     7141 gtattcctga ccgggtcgga tacctcatct aacgccctgt tcgccgcgct gcaagccacc
     7201 gcagcacaac aaattggcgt ctctgatctg ttgctggttg ccgccaatac caccggtggc
     7261 gtcaccggta agatgatctc cccgcaatct atcgctatcg cctgtgcggc ggtaggcctg
     7321 gtgggcaaag agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt
     7381 atggtgggcg tgatcaccac gcttcaggct tatgtcttaa cgtggatgat tccttaatga
     7441 ttgttttacc cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg
     7501 aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg ccaactggcg atgcaactcg
     7561 gcgtatcacg taattcacta cgcgaggcgc tggcaaaact ggtgagcgaa ggcgtgctgc
     7621 tcagtcgacg cggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa
     7681 acatcgtcca gccgctaaaa acgctgatgg ccgatgatcc ggattacagt ttcgatattc
     7741 tggaagcccg ctacgccatt gaagccagca ccgcatggca tgcggcaatg cgcgccacac
     7801 ctggcgacaa agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggata
     7861 tcgcctcaca agcggacgtt cgttttcatc tggcgattgc cgaagcctca cataacatcg
     7921 tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca atcctcagtg aagcatagcc
     7981 gtcagcggat gtatctggtg ccaccggttt tttcacaact gaccgaacaa catcaggctg
     8041 tcattgacgc catttttgct ggtgatgccg acggggcgcg taaagcaatg atggcgcacc
     8101 ttagttttgt tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc cacgcacgga
     8161 ttacccgcct gcccggtgag cataatgagc attcgaggga gaaaaacgca tgattatttc
     8221 cgcagccagc gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgttccacta
     8281 tatggatggt ggtgcatatt ctgaatacac gctgcgccgc aacgtggaag atttgtcaga
     8341 agtggcactg cgccagcgta ttctgaaaaa catgtccgac ttaagcctgg aaacgacgct
     8401 gtttaatgag aaattgtcga tgccggtagc actggcaccg gtgggtttgt gtggcatgta
     8461 tgcgcgtcgt ggcgaagttc aggcagccaa agcggcggac gcgcatggca ttccgtttac
     8521 tctctcgacg gtttccgttt gcccgattga agaagtcgcg ccagccatca agcgcccaat
     8581 gtggttccag ctttatgtac tgcgcgatcg cggctttatg cgtaacgcgc tggagcgagc
     8641 aaaagcagcg ggttgttcga cgctggtttt caccgtggat atgccgacac cgggcgcacg
     8701 ctaccgtgat gcgcattcag gtatgagcgg cccaaatgcg gcaatgcgcc gctacttgca
     8761 ggcggtgacg catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cacatgattt
     8821 aggtaatatc tcagcttatc tcggcaaacc gaccggactg gaagattaca tcggctggct
     8881 ggggaataac ttcgatccgt ccatctcatg gaaagacctt gagtggatcc gcgatttctg
     8941 ggatggcccg atggtgatca aagggatcct cgatccggaa gatgcgcgcg atgcagtacg
     9001 ttttggtgct gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtact
     9061 ctcttccgct cgtgcgttgc ccgctattgc agatgcggtg aaaggtgata tcgccattct
     9121 ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga
     9181 caccatactg ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc aggcgggtgt
     9241 agctaacctg ctaaatctga tcgaaaaaga gatgaaagtg gcgatgacgc tgactggcgc
     9301 gaaatcgatc agtgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc
     9361 tacggcactg gctccgatgg cgaaagggaa tgcggcatag tcgtttgcgc cccctcaccc
     9421 taaccctctc cctcagggag aggggaccgt tcggcgctgt atgtactccc tcactctgaa
     9481 acgacaccgc actctttttt tctccctcgc ccctccgggg agggccgggg tgaggggaaa
     9541 aggccgcacg gttcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
     9601 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg
     9661 cgctaatacc ggctttcgtc tacatatcat cgaaccgatg ggatttgcct gggacgacaa
     9721 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
     9781 ctatcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg
     9841 tacgcctgct cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga
     9901 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
     9961 tccgatggtg ccggacagcc gcagcatgaa tctgtccaat gcggtgtcgg tagtggtgta
    10021 tgaagcctgg cggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc
    10081 cggatagatg attacatcgc atccggcacg atcacaggac attagatccc atccccatac
    10141 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
    10201 tctggtttac cgacaatacg aaccggaacg ccagcggcgg ttgtatgcgg cggcaccggt
    10261 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgagg
    10321 attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca
    10381 gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc
    10441 gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg gccaattttt
    10501 gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
    10561 aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
    10621 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
    10681 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
    10741 tcaaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
    10801 atgtagctca gtgcactgcc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
    10861 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
    10921 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
    10981 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtgac tgctgcgctc
    11041 gtccttgcgt gcacgaccta ataatgtcaa tgctgcctcg cgcgcgtttt ttccgcaata
    11101 taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc
    11161 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
    11221 ttgtacatcc atgccctgac cgagcatcat accaaaacgg cggttacgcg actggttgtc
    11281 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
    11341 acccagcgcc gcaccaagac gcgacatttc agccagccca cgggtgatca gcgccgtacg
    11401 cgcattcgca ccaaaaccga taccgtcaga catccccgcg ccaatggcaa tgacgttttt
    11461 caccgcgccg ccaagctgca cgccaatgaa atccgggttg ctgtaaacgc ggaaactttt
    11521 gccgcagtgc agcagctgct ggagatcatc ggcaaaggtc tgatcggtcg aggccagcga
    11581 aatagctgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
    11641 cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc
    11701 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
    11761 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacgactggc
    11821 tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg
    11881 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
    11941 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
    12001 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
    12061 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc
    12121 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga
    12181 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
    12241 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
    12301 tcgcccaagg aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt
    12361 tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg
    12421 tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa
    12481 gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
    12541 tactgcgcaa tgtaatacca acgttcacac gttatttcag caggggatcc agtccaccac
    12601 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
    12661 ggggaaccgt ggtgcaaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat
    12721 cgatcggcaa ctcctggaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
    12781 aatacgggca ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt
    12841 tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat
    12901 aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg
    12961 taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca acgccctctt
    13021 tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact
    13081 gcatgccaga accgtctacc acgataaccg atttgtcttt gtgcttctca agctcaccaa
    13141 cattgttggc tttgatttcg ctcggcaaca gattgataga acctgcgata tggcctttac
    13201 ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg
    13261 tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa
    13321 gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt
    13381 gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa
    13441 ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc
    13501 ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt
    13561 ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc
    13621 ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg
    13681 atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg
    13741 taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag
    13801 tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg
    13861 gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct
    13921 ttgctaatcc agtgctggct ggcgcggtac ataaagcgaa aaacgcaggt aaagcagtac
    13981 acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga
    14041 tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg
    14101 gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg
    14161 ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg
    14221 ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt
    14281 tccaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg
    14341 agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg
    14401 gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat
    14461 tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga
    14521 tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg
    14581 ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa
    14641 ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag
    14701 gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa
    14761 tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca
    14821 ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg
    14881 cggttaaagc ggttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt
    14941 ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc
    15001 cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg
    15061 gtgataagaa cgtgaaagcg gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt
    15121 tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg
    15181 aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc
    15241 gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt
    15301 gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg
    15361 ccgcaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat
    15421 tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca
    15481 cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc
    15541 agcaaaaagc cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg
    15601 gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt
    15661 tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc
    15721 aaacgcgtga agaagtcacc atgcagcgtg ctgaactgga agagaaacaa agcgagcaac
    15781 aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgagc
    15841 gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg
    15901 aactgcgtgc caacgaatcc cgtctgcgta acagcattgc ccgtgcggaa gccgcggcga
    15961 aagcgcgtgc tgaacgagaa gcgcgtgagg cccaggcggt tcgcgagcgc cagaaagaag
    16021 cgacgcgcaa aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa
    16081 ctggtggcct gggtgcgccg cgtggtcagg cattctggcc ggttcgcggg ccgacgctgc
    16141 atcgctatgg cgagcagcta cagggtgaat tacgctggaa agggatggtt atcggtgcct
    16201 ctgaaggtac tgaagttaaa gcaattgccg acggtcgagt gattctggct gactggctgc
    16261 aaggttacgg tctggtggtg gtggttgagc acggtaaagg cgacatgagt ctttacggct
    16321 ataatcagag cgcactggtg agcgttggtt cgcaggttcg cgcgggccag ccaattgcac
    16381 tggtgggcag cagtggcggt cagggtcggc cttcactcta tttcgaaatt cgccgccagg
    16441 gtcaggcggt caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta
    16501 acgttcttgc atttgccgct ctgttggcgc tctcctcccc cgtacttgct ggcaaacttg
    16561 ccatcgtcat tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc
    16621 cttccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag atggcgacca
    16681 aagcgcataa cagcggtcac gaagtgttga ttcatctccc gatggcaccg ttgagtaaac
    16741 agccgctgga gaaaaatacg ctacgcccgg agatgagcag cgacgaaatt gagcgcatta
    16801 ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caacaaccac atgggcagca
    16861 agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcgctggag cgttacaatc
    16921 tttacttcct cgacagcgta accatcggta atacccaggc gatgcgcgcc gcgcaaggta
    16981 ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca
    17041 tccgtgtgca atttaatcgc gcaattgatc tggcgcgtcg caacggttca accattgcca
    17101 ttgggcatcc tcacccttca acggtacgcg tgctacaaca gatggtttat aacctgccgc
    17161 cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacttcta
    17221 caccgccgaa aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc gtgaagctgt
    17281 gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg
    17341 aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca
    17401 aacagcccga agcggcgaag tttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg
    17461 atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg
    17521 gtaaccgacc ttcacgctgc gcacattggt aatcaggcgc ttatacatac ccggaagtaa
    17581 atatctcggc aatcatccgt tggcgggtaa gaatatccgg ctctttgcgc acc
//
LOCUS       DAECNO010000084        17533 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.084, whole genome shotgun sequence.
ACCESSION   DAECNO010000084 DAECNO010000000
VERSION     DAECNO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.084"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            111..2201
                     /gene="pbpC"
                     /locus_tag="KKO08_002941"
                     /pseudo
     CDS             111..2201
                     /gene="pbpC"
                     /locus_tag="KKO08_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; incomplete; partial
                     in the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2351..2782
                     /gene="ndk"
                     /locus_tag="KKO08_002942"
     CDS             2351..2782
                     /gene="ndk"
                     /locus_tag="KKO08_002942"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBD6319110.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KKO08_002943"
     CDS             2932..4086
                     /gene="trmG/rlmN"
                     /locus_tag="KKO08_002943"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBD6319111.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4371..5384
                     /gene="rodZ"
                     /locus_tag="KKO08_002944"
     CDS             4371..5384
                     /gene="rodZ"
                     /locus_tag="KKO08_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBD6319112.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5411..6538
                     /gene="ispG"
                     /locus_tag="KKO08_002945"
                     /gene_synonym="gcpE"
     CDS             5411..6538
                     /gene="ispG"
                     /locus_tag="KKO08_002945"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBD6319113.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6641..7915
                     /gene="hisS"
                     /locus_tag="KKO08_002946"
     CDS             6641..7915
                     /gene="hisS"
                     /locus_tag="KKO08_002946"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBD6319114.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7933..8553
                     /gene="yfgM"
                     /locus_tag="KKO08_002947"
     CDS             7933..8553
                     /gene="yfgM"
                     /locus_tag="KKO08_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBD6319115.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8564..9742
                     /gene="bamB"
                     /locus_tag="KKO08_002948"
     CDS             8564..9742
                     /gene="bamB"
                     /locus_tag="KKO08_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBD6319116.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9860..11332
                     /gene="der"
                     /locus_tag="KKO08_002949"
     CDS             9860..11332
                     /gene="der"
                     /locus_tag="KKO08_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBD6319117.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11402..11617
                     /locus_tag="KKO08_002950"
     CDS             11402..11617
                     /locus_tag="KKO08_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBD6319118.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KKO08_002951"
     CDS             complement(11614..12984)
                     /gene="xseA"
                     /locus_tag="KKO08_002951"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBD6319119.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13146..14612
                     /gene="guaB"
                     /locus_tag="KKO08_002952"
     CDS             13146..14612
                     /gene="guaB"
                     /locus_tag="KKO08_002952"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBD6319120.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14681..16258
                     /gene="guaA"
                     /locus_tag="KKO08_002953"
     CDS             14681..16258
                     /gene="guaA"
                     /locus_tag="KKO08_002953"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBD6319121.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16353..16892)
                     /locus_tag="KKO08_002954"
     CDS             complement(16353..16892)
                     /locus_tag="KKO08_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBD6319122.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16908..17426)
                     /locus_tag="KKO08_002955"
     CDS             complement(16908..17426)
                     /locus_tag="KKO08_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBD6319123.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4074 a         4351 c         4863 g         4245 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacaggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca cgttaccttg
      121 aagcgctgat caattatgaa gatcgctggt tctggaagca tccgggggtt aatccattct
      181 cggtggcgcg cgcagcatgg caagatctca cttcgggacg ggttatttcc ggtggcagca
      241 cgctcactat gcaggttgct cgtctgcttg atcctcaccc caaaacattt ggtggcaaaa
      301 ttctccagct ctggcgcgcg ttgcaactgg agtggcattt gtctaagcgt gaaattctga
      361 tcttgtatct taaccgcgct ccgtttggcg gtacgttgca ggggatcggt gcggcaagtt
      421 gggcttatct cggaaaatcg cctgcgaatt taagctattc cgaggcggca atgctggcgg
      481 tgttaccgca agtgcccagc cgactgcgcc cggatcgttg gccggagcgt gccgaagccg
      541 cgcgtaataa agtgctcgaa cggatggctg cgcaaggtgt gtggtcccgt gagcaggtaa
      601 aagagtcaag ggaagaaccc atctggctgg caccccgaca aatgccgcaa ctggcaccgc
      661 tgttttcgcg catgatgctc ggtaaaagca aaagcgacaa aatcgttact acgctggatg
      721 ccggtcttca acgacgtctg gaagaactgg cgcaaaactg gaaagggcgg ttgccaccgc
      781 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      841 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      901 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      961 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
     1021 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
     1081 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1141 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1201 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1261 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1321 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1381 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1441 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1501 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1561 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1621 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1681 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1741 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1801 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1861 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1921 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1981 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     2041 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     2101 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2161 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2221 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2281 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2341 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2401 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2461 caaaatgctg catctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2521 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2581 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2641 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2701 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2761 agtgtgcccg cgcacctgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2821 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2881 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2941 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     3001 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     3061 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3121 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3181 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3241 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3301 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3361 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3421 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3481 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3541 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3601 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3661 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3721 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3781 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3841 tgtaagatca acctgatccc gtggaacccg ttcccggacg cgccgtatgg acgcagttcg
     3901 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3961 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     4021 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     4081 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4141 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4201 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4261 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4321 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4381 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4441 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4501 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4561 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4621 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4681 aacgccgcaa aaaacgcgac ggctggctaa tgaccttcac ctggctggtg ttgtttgtgg
     4741 ttatcggcct gagcggtgtc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4801 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4861 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4921 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4981 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     5041 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     5101 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5161 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5221 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5281 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5341 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5401 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5461 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5521 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5581 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5641 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5701 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5761 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5821 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5881 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5941 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     6001 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     6061 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6121 tgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6181 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6241 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6301 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6361 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6421 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6481 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt ggaaaaataa taacgtgatg
     6541 ggaagcgcct cgcttcccgt gtatgattga acccgcatgg ctcccgaaac attgagggaa
     6601 gcgttgaggg ttcattttta tattcagaaa gagaataaac gtggcaaaaa acattcaagc
     6661 cattcgcggc atgaacgatt acctgcctgg cgaaacggcc atctggcagc gcattgaagg
     6721 cacactgaaa aacgtgctcg gcagctacgg ttacagtgaa atccgcttgc cgattgtaga
     6781 gcagaccccg ctattcaaac gtgcgattgg tgaagtcacc gacgtggttg aaaaagagat
     6841 gtacaccttt gaggatcgca atggcgacag cctgactctg cgccctgaag ggacggcggg
     6901 ctgtgtacgc gccggcatcg agcatggtct tctgtacaat caggaacagc gtctgtggta
     6961 tatcgggccg atgttccgtc acgagcgtcc gcagaaaggg cgttatcgtc agttccatca
     7021 gttgggctgc gaagttttcg gtctgcaagg tccggatatc gacgctgaac tgattatgct
     7081 cactgcccgc tggtggcgtg cgctgggtat cttcgaacac gtaactcttg agctgaattc
     7141 tatcggttcg ctggaagcac gcgccaatta ccgcgatgcg ctggtggcat tccttgagca
     7201 gcataaagaa aagctggacg aagactgcaa acgccgcatg tacactaacc cactgcgcgt
     7261 gctggattca aaaaatccgg aagtgcaggc gcttctcaac gacgctccgg cattaggtga
     7321 ttatctggac gaggaatctc gtgagcattt tgccggtctg tgcaaactgc tggagagcgc
     7381 ggggatcgct tacaccgtaa accagcgtct ggtgcgtggt ctggattact ataaccgtac
     7441 cgttttcgag tgggtgacta acagtctcgg ctcccagggc accgtgtgtg caggcggtcg
     7501 ttatgacggt cttgtggaac aactgggcgg tcgtgcaaca ccggctgtcg gttttgcgat
     7561 gggcctcgaa cgtcttgtat tgttagtaca ggccgttaat ccggaattta aagccgatcc
     7621 tgttgtcgat atatacctgg tggcttcagg tgctgataca caatctgcgg ctatggcatt
     7681 agctgagcgt ctgcgtgatg aattactggg cgtgaaattg atgaccaacc acggcggcgg
     7741 caactttaag aaacagtttg cccgtgctga taaatggggt gcccgcgttg ctgtggtgct
     7801 gggtgagtct gaagtggcta acggcacagc agtagtgaag gatttgcgct ctggtgagca
     7861 aacggcagtt gcgcaggata gcgtagccgc gcatttgcgc acgttactgg gttaaggaag
     7921 gagaaggaca gcgtggaaat ttacgagaac gaaaacgacc aggtagaagc ggttaaacgc
     7981 ttttttgctg aaaatggcaa agcactggct gttgggggga ttttgggcgt tggcgcactg
     8041 attggctggc gctactggaa cagccatcag gttgattctg cacgctccgc ttctcttgcc
     8101 tatcaaaatg cggttaccgc agtgagcgaa ggcaaaccgg atagcatccc ggcggcggaa
     8161 aaatttgctg ctgaaaataa aaatacttat ggtgcgctgg cttctttgga acttgcgcag
     8221 caatttgttg acaaaaatga actagagaaa gctgccgccc agttacaaca ggggctggca
     8281 gacacgagcg atgaaaatct caaagccgtg ataaatctgc gtcttgctcg cgttcaggta
     8341 cagctcaagc aggctgatgc cgcgctgaaa acccttgata ccatcaaagg tgaagggtgg
     8401 gctgccattg ttgccgactt gcgtggtgaa gcattgctga gcaaaggtga taagcaaggt
     8461 gcgcgtagtg catgggaagc aggcgtgaaa agcgatgtta ctccggcact gagcgaaatg
     8521 atgcagatga aaattaataa tttgtccatc tgagagggac ccgatgcaat tgcgtaaatt
     8581 actgctgcca ggactgcttt ccgttaccct tttaagcggc tgttcgctgt ttaacagcga
     8641 agaagatgtg gtaaagatgt ccccattgcc aaccgttgaa aaccagttta cgccgaccac
     8701 ggcgtggagc acttccgttg gtagcggcat tggcaacttc tattccaatc ttcatccggc
     8761 actggcggac aacgttgtct atgcagcgga ccgcgctggt ttggtaaaag cgctgaatgc
     8821 ggatgatggc aaagaaatct ggtctgtcag cctggccgag aaagatggct ggttctctaa
     8881 agagcctgca ttactttctg gcggtgtgac cgtgtctggt gggcatgtct acattggcag
     8941 cgaaaaggcg caggtttacg cgctgaatac cagcgatggt actgtggcat ggcaaactaa
     9001 agtcgcgggt gaagcacttt cgcgcccggt ggtcagcgac ggtctggtgt taatccacac
     9061 cagtaacggt cagttacaag cgctgaacga agctgacggc gctgtcaaat ggacagttaa
     9121 cctcgatatg ccttcgctct ctttgcgtgg cgagtctgcg ccggcaacgg cttttggtgc
     9181 ggccgtcgtg ggtggcgata atggtcgcgt cagcgcagtg ctgatggaac agggccagat
     9241 gatttggcag cagcgtattt cccaggcgac cggttctacc gaaattgacc gtctgagcga
     9301 tgttgacacg actcccgtcg ttgttaacgg cgttgttttc gcgctggcct ataatggtaa
     9361 cctgacggcg cttgatctgc gcagtggtca gattatgtgg aaacgcgaac tgggttcggt
     9421 gaatgatttc atcgtcgatg gcaatcgcat ctatctggtc gatcaaaatg accgggtgat
     9481 ggcgttgacc attgatggcg gcgttacgct gtgggcacaa agcgatctgc tgcatcgcct
     9541 gctgacttct ccggtgctgt ataatggcaa cctggtggtc ggtgacagtg aaggttatct
     9601 gcactggatt aacgtcgaag atggtcgttt cgttgcccag caaaaagttg atagttccgg
     9661 tttccagact gaaccggttg ccgctgacgg caaactgctg atccaggcaa aagacggaac
     9721 cgtgtactct attacacgtt aatcgtctct gtcgttcact ttgaaaacgg ctcctggaca
     9781 ggggccgttt tcctgttttt aacaacgacg cgaatatagc gtgcgttgtc tgatgattta
     9841 taaaaatgag gctttaaaca tggtacctgt ggtcgcgctt gtcgggcgcc ctaacgtagg
     9901 aaaatccacg ttatttaacc gtctaactcg cacccgagat gcgctggttg cggatttccc
     9961 gggtctgact cgtgaccgta agtacggtcg tgcggaaatt gaaggccgtg agtttatctg
    10021 tattgatacc ggcgggattg atggcacaga agacggtgta gaaacccgca tggcggaaca
    10081 gtcgctgctg gcgattgaag aagcggacgt cgtactgttt atggtggatg cgcgcgcggg
    10141 cctgatgccg gcagatgaag cgattgccaa acatctgcgc tcccgtgaaa aaccgacctt
    10201 cctggtggta aacaaaactg acggtctgga tcccgatcag gcagtggttg atttctactc
    10261 gcttggttta ggtgaaatct acccgatcgc cgcgtctcac ggtcgtggcg tattaagtct
    10321 gctggagcat gtgctgctgc cgtggatgga agatctcgca ccgcaagagg aagtcgacga
    10381 agacgctgaa tactgggcgc aatttgaagc ggaagagaac ggcgaagaag aagaggaaga
    10441 cgacttcgac ccgcaaagtc tgccgatcaa actggcgatt gtgggtcgtc cgaacgtagg
    10501 taagtctaca ctcactaacc gtattcttgg agaagagcgc gttgttgttt acgacatgcc
    10561 tggcacgacg cgtgacagca tctacatccc aatggaacgc gatggacgtg agtatgtgct
    10621 cattgacacc gctggcgtac gtaaacgcgg caaaatcacc gatgctgtag agaaattctc
    10681 cgtaatcaaa acgttgcagg ccattgaaga cgccaacgtg gtgatgttag tgattgatgc
    10741 gcgcgaaggt atttccgatc aggatctctc gctgctgggc tttattctca atagtgggcg
    10801 ctcacttgtc attgtggtga ataagtggga tggcctgagc caggaagtga aagagcaggt
    10861 gaaagaaacg ctggacttcc gtctgggctt tatcgatttt gctcgtgtgc actttatctc
    10921 tgccttgcac ggcagtggtg ttggtaactt gtttgaatca gtacgtgaag cgtatgacag
    10981 ctccacccgt cgtgtgggga cctctatgct gacgcgcatc atgacgatgg ctgttgaaga
    11041 tcaccaaccg ccgctggtac gcggtcgtcg tgtgaagctg aaatatgccc acgctggtgg
    11101 ttataacccg ccgattgtgg tgattcacgg taatcaggtg aaagacctgc ctgattccta
    11161 caagcgctac ttgatgaact acttccgcaa atcgctggac gtaatgggat cgccgattcg
    11221 tattcagttc aaagaagggg aaaacccgta tgcgaacaag cgtaacaccc tgacgccaac
    11281 ccagatgcgt aaacgtaagc gtctgatgaa gcacatcaag aaaaataaat aattatccgt
    11341 aagtatttta tctgccattc cctctatgaa tgtagaggga atggctaccg gaggaattca
    11401 tatggaactg cattgtccaa aatgtcagca tgtgcttgat caggataacg gccatgcgcg
    11461 ttgcccatcc tgcggggaat ttattgaaat gaaagctctt tgtccagact gccatcaacc
    11521 gttgcaggtg ttaaaagcct gcggtgcagt agattacttc tgtcagcacg ggcatggctt
    11581 gatatcgaaa aaacgcgttg aattcgtgct ggcttaatga acctttttac gcgatttttt
    11641 taccggctgg atgtttttta cttcactttc tatccagccg tcttccagac gtgtggttag
    11701 catttcaccc gttttaactt gcttcacttt tttcagtaca ttgccgtcag tagcagtagt
    11761 aacgctgtat ccacgcgcca gcgttgacag tgggcttacg gcttcgaggt gcgttactgc
    11821 attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc aggatttctg ctaaacgata
    11881 ttccagttgc tgaatgcgcg tttgcgtgcg atgaatcttc ggctgtggat tttgctgatt
    11941 cagccgctgt gttaatcgct gctgctgttg cccggcacgc ttaagctgat tttccagcgc
    12001 aaagctcatc cgtttttgca ggcgttcaag catggtttgc tggcgtgcca gccggagctg
    12061 cggatgctgc tgctgcaagc gatgatgaat ctgcgtaaag cgacgtgtac ggttggcgag
    12121 ataataatcc atcgccatct ccagccgttg acgggccgat tgcacctggc gcagtaactc
    12181 ttgctgatta cggctcacta cttcagcggc ggcagacggc gttggcgcac gcagatcggc
    12241 aacaaaatcg gcaatggtca catccgtctc atgcccgacg gcgctgacga ccggaatgcg
    12301 gctggcaaaa atcgcccgcg ctacgcgttc gtcgttaaaa ctccataaat cttccagcga
    12361 accgccgccg cgcccaacga tcaacacgtc gcactcattg cgctgattcg ccagttcaat
    12421 ggcgcgaaca atttgccccg gcgcgtcatc gccctgaacg gaggtggggt agatgatcac
    12481 cggtagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg cagcaccggt
    12541 ttttgaggtg atcacaccaa cgcaatgcgc aggggaggga agtggttttt tgtattgctg
    12601 atcgaacaaa ccttcagcct gcaacttcgc tttgagctgt tcgtacttct gttgcagcag
    12661 cccttcaccg gccggctgca tactctcaac gattatctgg tagtcgccgc gcggctcgta
    12721 gagcgtaata ttggcgcgaa ctaaaacttg ttgcccatgc tgcggacgga aggtgacccg
    12781 gcggttgctg ttgcggaaca tcgcgcagcg tacctgggcg gtgtcgtctt tgagtgtaaa
    12841 gtaccagtga ccggaagccg gttgtgtgaa attagagatt tcgccgctga tccaaacctg
    12901 tcccatctca tgctcaagca gcagacgaac cgtttgattc aggcgactaa cggtaaaaat
    12961 tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag caggttattc
    13021 agtcgatagt aacccgccct tcggggatag caagcatttt ttgcaaaaag gggtagatgc
    13081 aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg gtcgagatat
    13141 tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc ctcgttcctg
    13201 ctcattctac cgttctgccg aatactgctg acctcagcac ccagctgacg aaaactattc
    13261 gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg cgcctggcta
    13321 ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc attgaacgcc
    13381 aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg tgtggtgact gatccgcaga
    13441 ctgttctgcc aaccacgacg ctgcgcgaag tgaaagaact gaccgagcgt aacggttttg
    13501 cgggctatcc ggtcgttacc gaagaaaacg aactggtggg tattatcacc ggtcgtgacg
    13561 tgcgttttgt taccgacctg aaccagccgg ttagcgttta catgacgccg aaagagcgtc
    13621 tggtcaccgt gcgtgaaggt gaagcccgtg aagtggtgct ggcaaaaatg cacgaaaaac
    13681 gcgttgaaaa agcgctggtg gttgatgacg aattccacct gatcggcatg atcaccgtga
    13741 aagacttcca gaaagcggaa cgtaaaccga acgcctgtaa agacgagcaa ggccgtctgc
    13801 gtgttggtgc tgcagttggc gcaggtgcgg gtaacgaaga gcgtgttgac gcgctggttg
    13861 ccgctggcgt tgacgttctg ctgatcgact cctcccacgg tcactcagaa ggcgttttgc
    13921 agcgtatccg tgaaacccgt gctaaatatc cggatctgca aatcatcggc ggcaacgtgg
    13981 caacagctgc aggtgcacgc gctttggcag aagctggttg cagcgcggtt aaagtcggta
    14041 tcggccctgg ttctatctgt acgactcgta tcgttactgg cgtaggtgtt ccgcagatca
    14101 ctgccgttgc tgacgcagtt gaagccctgg aaggcaccgg tattccggtt atcgcagatg
    14161 gtggtattcg cttctccggc gacatcgcca aagctatcgc cgctggcgca agcgcggtga
    14221 tggtaggttc catgctggcg ggtactgaag aatctccggg tgaaatcgaa ctctaccagg
    14281 gccgttctta taaatcttac cgtggtatgg gttccctggg cgcgatgtcc aaaggttcct
    14341 ctgaccgtta tttccagagc gataacgctg ccgacaaact ggtgccggaa ggtatcgaag
    14401 gtcgcgtagc ttataaaggt cgcctgaaag agatcattca ccagcagatg ggcggcctgc
    14461 gctcctgtat gggtctgacc ggctgtggta ctatcgacga actgcgtact aaagcggagt
    14521 ttgtacgtat cagcggtgcg ggcattcagg aaagccacgt tcacgacgtg accattacta
    14581 aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgatttc atgtcgggcg
    14641 atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa acattcataa
    14701 gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg cgcgccgcgt
    14761 gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag cacaaattcg
    14821 tgacttcaat ccaagcggaa ttattctttc cggcggcccg gaaagtacta ctgaagaaaa
    14881 cagtccgcgt gcgccgcagt atgtctttga agcaggcgta ccgatattcg gcgtttgcta
    14941 tggcatgcag accatggcaa tgcagttggg cggtcacgtt gaagcctcta acgaacgtga
    15001 atttggctac gcgcaggtag aagtcgtaaa cgacagcgca ctggttcgcg gtatcgaaga
    15061 tgcgctgacc gcagacggta aaccgctgct cgatgtctgg atgagccacg gcgataaagt
    15121 taccgctatc ccgtccgact tcgtcaccgt agccagcacc gaaagctgcc cgtttgccat
    15181 tatggctaac gaagaaaaac gcttctatgg cgtacagttc cacccggaag tgactcacac
    15241 ccgccagggt atgcgcatgc tggagcgttt tgtgcgtgat atctgccagt gtgaagccct
    15301 gtggacgcca gcgaaaatta tcgacgatgc tgtagctcgc atccgcgagc aggtaggcga
    15361 cgataaagtc atcctcggcc tctctggtgg tgtggattcc tccgtaaccg caatgctgct
    15421 gcaccgtgct atcggtaaaa acctgacttg cgtattcgtc gacaacggcc tgctgcgtct
    15481 caacgaagca gagcaggttc tggatatgtt tggcgatcac tttggtctta acattgttca
    15541 cgtaccggca gaagatcgct tcctgtcagc gctggctggc gaaaacgatc cggaagcaaa
    15601 acgtaaaatc atcgggcgcg ttttcgttga agtattcgat gaagaagcgc tgaaactgga
    15661 agacgtgaag tggctggcgc agggcaccat ctaccctgac gttatcgaat ctgcggcttc
    15721 tgcaaccggt aaagcacacg tcatcaaatc tcaccacaac gtgggcggcc tgccgaaaga
    15781 gatgaagatg ggcctggttg aaccgctgaa agagctgttc aaagacgaag tgcgtaagat
    15841 tggtctggag ctgggcctgc cgtacgacat gctgtaccgt catccgttcc cgggaccagg
    15901 ccttggcgtt cgtgttctgg gtgaagtgaa gaaagagtac tgtgacctgc tgcgccgtgc
    15961 tgacgccatc ttcattgaag aactgcgtaa agcggacctg tacgacaaag tcagccaggc
    16021 gttcaccgtc ttcctgccag tacgttccgt tggcgtaatg ggcgatggtc gtaagtatga
    16081 ctgggttgtc tctctgcgtg ctgtcgaaac catcgacttt atgaccgcac actgggcgca
    16141 tctgccgtac gatttcctcg gtcgcgtttc caaccgcatt atcaatgaag tgaacggtat
    16201 ttcccgcgtg gtgtatgaca tcagcggcaa gccgccagct accattgagt gggaatgatt
    16261 ttgatccctg cactatgaat gaacaaaacc ctccgttact acagagggtt ttttatcttc
    16321 aagaatcata ggcttgaagt tactaacatc gattaattaa accagctgtc cgatttgttc
    16381 tcttctgctt tgcccacgct tttcatcaga tcgcgaccgc cttcagtcat atttctgttt
    16441 gcgtcagctt cagattgcac cacatcggtt tgcgcagctt tgtgcttcag ttcctgatcg
    16501 ataaattcgt tttcgcgctt aacgcgggct tcttctttcg ccagcgccag tttttgtttc
    16561 tgaatctcta agctgcgtag ctcatcttca taactttgat cgcgtttttt gtccgcagtg
    16621 gcttcggcgt ccagtttatc ctgacgagct ttcttatttg ccgctgccgt tgccgctctt
    16681 ttgttcgcgg cggcctgggc atttgcgcga cgttgcttct cttgctggat ttccctgttg
    16741 cgctccgcga cccattcgtc atgctgcctt tgctcttcat ttttaccttg ctgttccgct
    16801 tctgcgacag cagagagttg atcctgcaat gatgaggcta tagccggata gcttaaggag
    16861 gctaagatgg cgcaaagaaa aactttcttc atgactcctc ctgattatta gctcttttca
    16921 ggacatttgg tatttggctg aatacgcgtt tcgttatacg ttgtggtaat aacaacggct
    16981 aaacctgtcg taaactggca ctctttaccc tcctgggtgg aggtatacac tttggtgcct
    17041 tccttatatg ttaaagaaac accttccact aaggttttat cattcaccat agaacccgct
    17101 gccgcgccta cagctccgcc gccaactgcc cctgccgtcg ttccggaatt gctgccagaa
    17161 ccgacgttgt ggccgataac accgccagcg actgcgccaa taagcgcgcc gaaggcttgt
    17221 gcgttccgtt tattttggga gttgtctacg gcaacttttg cgggaagaat ggaaataata
    17281 ttaacggttt tagtttcttg tttggtattc agttgatcgg tttgataaac atcggcggca
    17341 tgatcgtcag catttgactg gcatcctgcc agagtgaatg acgctaacat tgccacaggc
    17401 agaagacatt ttttaaattt catcactatt ccttgttata cacattacga aaaataagac
    17461 atttaagatc atcaaaagag gaaagtaaat gtattgcgta taaagggata gattaaattt
    17521 attaaaattc acg
//
LOCUS       DAECNO010000085        17478 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.085, whole genome shotgun sequence.
ACCESSION   DAECNO010000085 DAECNO010000000
VERSION     DAECNO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.085"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..193)
                     /locus_tag="KKO08_002956"
     CDS             complement(<1..193)
                     /locus_tag="KKO08_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6319124.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVV"
     gene            complement(385..639)
                     /locus_tag="KKO08_002957"
     CDS             complement(385..639)
                     /locus_tag="KKO08_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBD6319125.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDTPVTLFVPLIMGC"
     gene            949..2721
                     /gene="aspS"
                     /locus_tag="KKO08_002958"
     CDS             949..2721
                     /gene="aspS"
                     /locus_tag="KKO08_002958"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBD6319126.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDDEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2839..3291
                     /gene="nudB"
                     /locus_tag="KKO08_002959"
     CDS             2839..3291
                     /gene="nudB"
                     /locus_tag="KKO08_002959"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBD6319127.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3320..4060
                     /locus_tag="KKO08_002960"
     CDS             3320..4060
                     /locus_tag="KKO08_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD6319128.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4095..4616
                     /gene="ruvC"
                     /locus_tag="KKO08_002961"
     CDS             4095..4616
                     /gene="ruvC"
                     /locus_tag="KKO08_002961"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBD6319129.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4618..5220)
                     /locus_tag="KKO08_002962"
     CDS             complement(4618..5220)
                     /locus_tag="KKO08_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBD6319130.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            5289..5354
                     /gene="yobI"
                     /locus_tag="KKO08_002963"
     CDS             5289..5354
                     /gene="yobI"
                     /locus_tag="KKO08_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBD6319131.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5493..6104
                     /gene="ruvA"
                     /locus_tag="KKO08_002964"
     CDS             5493..6104
                     /gene="ruvA"
                     /locus_tag="KKO08_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBD6319132.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            6113..7123
                     /gene="ruvB"
                     /locus_tag="KKO08_002965"
     CDS             6113..7123
                     /gene="ruvB"
                     /locus_tag="KKO08_002965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBD6319133.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7270..8055)
                     /gene="znuB"
                     /locus_tag="KKO08_002966"
     CDS             complement(7270..8055)
                     /gene="znuB"
                     /locus_tag="KKO08_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBD6319134.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(8052..8807)
                     /gene="znuC"
                     /locus_tag="KKO08_002967"
     CDS             complement(8052..8807)
                     /gene="znuC"
                     /locus_tag="KKO08_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBD6319135.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8886..9818
                     /gene="znuA"
                     /locus_tag="KKO08_002968"
     CDS             8886..9818
                     /gene="znuA"
                     /locus_tag="KKO08_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBD6319136.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9834..11156
                     /gene="mepM"
                     /locus_tag="KKO08_002969"
     CDS             9834..11156
                     /gene="mepM"
                     /locus_tag="KKO08_002969"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBD6319137.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            11276..12247
                     /gene="lpxM"
                     /locus_tag="KKO08_002970"
                     /gene_synonym="msbB"
     CDS             11276..12247
                     /gene="lpxM"
                     /locus_tag="KKO08_002970"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBD6319138.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12378..13820)
                     /gene="pyk"
                     /locus_tag="KKO08_002971"
     CDS             complement(12378..13820)
                     /gene="pyk"
                     /locus_tag="KKO08_002971"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBD6319139.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13948..14817)
                     /gene="hexR"
                     /locus_tag="KKO08_002972"
                     /gene_synonym="yebK"
     CDS             complement(13948..14817)
                     /gene="hexR"
                     /locus_tag="KKO08_002972"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBD6319140.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15154..16629
                     /gene="zwf"
                     /locus_tag="KKO08_002973"
     CDS             15154..16629
                     /gene="zwf"
                     /locus_tag="KKO08_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBD6319141.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16864..>17478
                     /locus_tag="KKO08_002974"
     CDS             16864..>17478
                     /locus_tag="KKO08_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBD6319142.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRM"
BASE COUNT         4458 a         4316 c         4543 g         4161 t
ORIGIN      
        1 caacaaccag cagtatctga ccatgttgtt tcaggtcaga tattagcgac ctgagtttgt
       61 tttcgtcgtt gggtaatgct ttatcgaaca ggcgtttacc tgaacgatta atggcaacag
      121 cgtgatgcgt atctttaccg acatcaacgc cgataaagac ctggacggat tcgtaatcgc
      181 tggattcggt cattctgtct cccttgtata tgggttaacc agataaccac ggggagcagg
      241 taccggcatc cacgttacag acggtcccgg caaaagtgcc tgacctgacc cctattagcg
      301 gttaccagcg ccccaccaga cccggtgaca tcaccccccg gatcatggac gactgggggc
      361 agtaatcatg ccgggtctgg ctggctaaca ccccattata aggggtacga ataaagtaac
      421 gggggtatca accggctgtt ttaatgcttc ggcgtaatcg gcagaccagc caactcgcac
      481 cagaaacacg ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg cgcgattaac
      541 tacctcatcg gcagtatgtg gaccaccggc aaaaggtaag atgccttctt gtaaatcgat
      601 caccaccaac gcagtggttt tagcattaag ttcaagcatg ctgactcctg aaaaaacaaa
      661 atcgtgcctc acaccttaat gcgattagcc gatggcggaa cgacaaattt tgttaatttt
      721 tatgagttcg ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc aggcccgata
      781 taaagtggta acgaggcgtt ccagcgcggg ccaggctctt acggtgcggc agaatttcca
      841 gtataatagc cgcctttttt cattcagttg tgacatacag ctaacgctgc gacttggtca
      901 cctgcgcagc agggggcatc cgccggcagc taagttaagg gatatctcat gcgtacagaa
      961 tattgtggac agctccgttt gtcccacgtg gggcagcagg tgactctgtg tggttgggtc
     1021 aaccgtcgtc gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg cgaaggtatc
     1081 gtgcaggtat ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc tgaactgcgt
     1141 aatgagttct gcattcaggt tacgggcacc gtacgtgcgc gtgacgaaaa aaatattaac
     1201 cgcgatatgg cgaccggcga aattgaagtg ctggcgtcct cgctgactat catcaaccgc
     1261 gcagatgttc tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg tctgaaatac
     1321 cgctacctcg acctgcgtcg cccggaaatg gcgcagcgcc tgaaaacccg cgctaaaatc
     1381 accagcctgg tgcgccgttt tatggatgac cacggcttcc tcgacatcga aactccgatg
     1441 ctgaccaaag ccacgccgga aggcgcgcgt gactacctgg tgccttctcg tgtgcacaaa
     1501 ggtaaattct acgcactgcc gcaatcccca cagttgttca aacagctgct gatgatgtcc
     1561 ggttttgacc gttactatca gatcgttaaa tgcttccgtg acgaagacct gcgcgctgat
     1621 cgtcagcctg aatttactca gatcgatgtg gaaacttctt tcatgaccgc gccgcaagtg
     1681 cgtgaagtga tggaagcgct ggtgcgtcat ttgtggctgg aagtgaaggg cgtggatctg
     1741 ggcgatttcc cggtaatgac ctttgcggaa gcagaacgcc gttatggttc tgataaaccg
     1801 gatctgcgta acccgatgga actgaccgac gttgccgatc tgctgaaatc tgttgagttt
     1861 gcagtatttg cggggccggc gaacgatccg aaaggccgtg ttgccgctct acgtgttccg
     1921 ggtggcgcat cgctgacccg taagcagatc gacgaatacg gtaacttcgt taaaatctac
     1981 ggcgcgaaag gtctggctta catcaaagtt aacgaacgcg cgaaaggtct ggaaggtatc
     2041 aacagcccgg tagcgaagtt ccttaatgca gaaatcatcg aagccatcct ggatcgtact
     2101 gccgcgcaag atggcgatat gattttcttc ggtgccgaca acaagaaaat tgttgccgac
     2161 gggatgggcg cactgctcct gaaagtgggt aaagaccttg gtctgaccga cgaaagcaaa
     2221 tgggcaccgc tgtgggttat cgacttcccg atgtttgaag acgacgatga aggcggcctg
     2281 acggcaatgc accatccgtt cacctcaccg aaagatatga ctgctgcaga actgaaagct
     2341 gcaccggaaa atgcggtggc gaacgcttac gatatggtca tcaatggtta cgaagtgggc
     2401 ggtggttcag tacgtatcca taatggtgat atgcagcaga cggtgtttgg tattctgggt
     2461 atcaacgaag aggaacagcg cgagaaattc ggcttcctgc tcgacgccct gaaatacggt
     2521 actccgccgc acgcaggtct ggcattcggt cttgaccgtc tgaccatgct gctgactggc
     2581 accgacaata tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc gtgtctgatg
     2641 actgaagcac cgagctttgc taacccgact gcactggctg agctgagcat tcaggttgtg
     2701 aagaaggctg agaataactg atatgactca aatacacgaa atcattcgcg ttgcatcaag
     2761 gcggcaactg agtgaactcc catgagcata gataactatg tgaatgggat gagcgaaggc
     2821 agtcaacgaa gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc gatcttagtg
     2881 gtcatctatg cacaagatac gaaacgggtg ctgatgttgc agcggcgtga cgatcccgat
     2941 ttctggcagt cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca agctgccatg
     3001 cgcgaagtaa aggaagaggt taccattgat gttgtcgctg aacaactgac cttaattgac
     3061 tgtcagcgca cggtagagtt tgaaattttt tcacatttac gtcatcgcta tgcgccgggc
     3121 gtgacgcgta atacggaatc atggttctgt cttgcgcttc cgcacgagcg gcagatcgtt
     3181 ttcactgaac atctggctta caagtggctt gatgcgcctg ctgcggcggc gctcactaag
     3241 tcctggagca accggcaggc gattgaacag tttgtaatta acgctgcctg aacaggcagg
     3301 gctatctgga gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa
     3361 agctgcgcag gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac
     3421 cgcggctaag ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg cggcggtgga
     3481 taaagcactg tctaacaaca tgacccgtga cacactgaac cgcgcaattg cacgtggtgt
     3541 gggcggtgat gatgatgcaa acatggaaac catcatctac gaaggttacg gtcctggcgg
     3601 cacggcaatc atgattgaat gtctgtctga caaccgcaac cgtaccgttg ctgaagtgcg
     3661 tcacgcattc agcaaatgtg gcggtaacct cggcactgac ggttccgtag cttatctgtt
     3721 cagcaaaaaa ggcgtgatct ccttcgagaa aggcgacgaa gacaccatca tggaagcagc
     3781 tctggaagca ggtgctgaag acgttgtgac atatgatgac ggcgcgattg atgtctacac
     3841 tgcatgggaa gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc tgaaagcaga
     3901 cagcgcggaa gtttccatga tcccgtccac caaagctgat atggatgcag aaaccgcgcc
     3961 gaaactgatg cgtctgatcg atatgctgga agattgcgac gacgtgcagg aagtttacca
     4021 taacggtgaa atctctgatg aggttgcagc gactctctga tgatgcctgc taaacagcaa
     4081 aacggagacg cgtgatggct attattctcg gcattgaccc gggttcgcgc gtgaccggct
     4141 acggcgtcat ccgccaggta ggtaggcaac tgtcctacct gggtagcgga tgcatacgca
     4201 ccaaagtgga tgatttaccg tctcgtctga agctcatcta tgcgggcgtg acggaaatca
     4261 tcacccagtt ccagcctgat tatttcgcca ttgaacaagt ctttatggcg aagaacgctg
     4321 actcagccct gaaactgggc caggcgcgcg gcgtggcgat tgttgcggcg gtgaatcagg
     4381 aattgccggt atttgaatac gcggcacgtc aggtaaagca aacggtggta ggtatgggta
     4441 gtgccgaaaa aagccaggtg cagcatatgg tccgcacctt gctgaaactg cccgctaatc
     4501 cacaggcgga tgccgccgat gcgctggcga ttgccatcac ccactgccac gttagtcaga
     4561 atgcgatgca gatgagcgaa tcgcggctga acctgacgag agggcgactg cgttaagtta
     4621 taccgcctcg gtcagttccg gctgaggcgt ttcaactccc tccgcgtgaa tcaacaccaa
     4681 acccggatga tgccacaaac tggctggcgt gacggtttta cgctcccacg gaatagaacc
     4741 taagaaccag aatttccaga aagtgagttt tgcattcgga ttgctattta acagttgtcc
     4801 aaacgtctct atttcaccca ccggaataca tagcgcctct ttataaatat caaagacaaa
     4861 ttttgaacag aactggcgcg aagattcgta tttaaaaccc gtgtggtaaa gtttgcgtag
     4921 ccgggaggga acctgttcaa caattcgttg tttttgttgt tctgtcagcc cagcgtctaa
     4981 tcgctttata gcatagcgtt gattagacga acgtttaata aaacgagata gcgtggtgat
     5041 ggttgaaagg ggaacccggc tttctgcaac caggaagtct tcaccgttat gaccgataat
     5101 gatcccgacg tgattactcc agcaatttga tgcagctgat atttgaccaa ataaggcagc
     5161 acctatacat gtaaagacga tatctccaat ttcatattca gcagggtaat taatattcac
     5221 gttgtcattc cattgaaata gatacgaata tacgtaatag cataaaaaat tatttttcag
     5281 gagtcgcaat gtatattttt attacgcatt tcttcactga atatgtaata ttcaaatatt
     5341 tgcttccaat ataacctgtt gaataaatta tactgtgcca tttttcagat catcgggaca
     5401 cctcgcaagt tttcttcatc cttcgctgga tatctatcca gcattttttt atcatacagc
     5461 attatctttg attcattacg caggagcgtc atgtgatagg cagactcaga ggcatcatca
     5521 ttgaaaaaca acccccgctg gtgttaattg aagtgggcgg cgtaggctat gaagtgcata
     5581 tgccgatgac ctgtttttat gaactccctg aagcgggtca ggaagcgatc gttttcaccc
     5641 actttgtggt gcgtgaagac gcgcaactgc tgtacggttt taacaataaa caagaacgca
     5701 cgttgttcaa agagttgatc aaaaccaacg gcgtcggccc gaagttggcc ctggcgatcc
     5761 tctccggaat gtcagcgcag cagttcgtta atgccgttga gcgtgaagaa gtgggggcac
     5821 tggtgaaact gccgggtatt ggcaaaaaaa ccgccgaacg cttgattgtt gaaatgaaag
     5881 accgatttaa aggtttgcat ggcgatctct ttacgccagc cgccgacctg gtactcacgt
     5941 ctcctgctag cccggcgacc gacgatgctg aacaagaagc agttgccgcg ctggtggcgc
     6001 taggctataa accacaagaa gcaagccgca tggtgagcaa aatcgctcgc cctgacacca
     6061 gcagtgaaac attaattcgc gaagccctac gcgccgcgtt atgaggtaaa ggatgattga
     6121 agcagaccgt ctgatttctg ccggtaccac tttgccggaa gatgtagcag atcgcgccat
     6181 tcgccctaaa ttactggaag agtatgttgg tcagccgcag gttcgttcac agatggagat
     6241 tttcatcaaa gcagcgaaac tgcgcggcga tgccctcgat catttgttga tttttggtcc
     6301 tccggggttg ggtaaaacta cgcttgccaa cattgtcgcc aatgaaatgg gcgttaattt
     6361 acgcacgact tctggtccgg tgctggaaaa ggcgggcgat ttggctgcga tgctcactaa
     6421 ccttgaaccg catgacgtgc tgtttattga tgagatccac cgtttatcgc cagttgttga
     6481 agaagtgctg tacccggcaa tggaagacta ccaactggat atcatgattg gtgaaggtcc
     6541 ggcggcacgc tccattaaaa ttgatttgcc gccgtttacc ctgattggcg caaccacgcg
     6601 cgcaggttcg ctgacatcac cgttgcgcga ccgttttggt attgtgcaac gtctggagtt
     6661 ttatcaggtg ccggatctgc aatatatcgt cagtcgcagt gcacgcttta tggggcttga
     6721 gatgagtgat gacggtgcgc aggaagtagc tcgtcgcgct cgcggtacgc cacgtattgc
     6781 caaccgtctg ctgcgtcgag tgcgtgattt cgccgaagtg aagcacgatg gcaccatctc
     6841 ggcagatatc gctgctcagg cgctggatat gttgaatgtc gatgctgaag gtttcgatta
     6901 tatggaccgc aaattgttgc tggcggtaat cgataagttc tttggtgggc cggtaggtct
     6961 ggataacctg gcggcagcca ttggcgaaga acgtgaaacc attgaagatg tactggaacc
     7021 ttatttgatt cagcaaggct ttttgcagcg tacaccgcgc gggcgtatgg cgacgacgcg
     7081 ggcgtggaat cactttggca taacgccgcc agaaatgccg taagtcggat tggtcgctcg
     7141 cgccgcatcc gacactggca gtgtgtctga tgcgacactg ttcgcgtctt atcaggccag
     7201 ccagagatag cgaaccgtag gtcggataag gcgctcgcgc cgcatccgac aaatgtgttc
     7261 agcgatagat tagctggcct gctttttcat catactgata ataaataaca gtgccgcaca
     7321 tagcaccacc gacggacccg ccggcgtatc gtaaaacgcg gaaaaggtta aaccgccagt
     7381 tactgccacc atccccacca aaacagcgac accagccatc tgttccggcg tacgggcaaa
     7441 gcgacgcgca gtagctgcag gaataatcag caacgaagta ataatcaacg caccgacaaa
     7501 tttcatcgct acaccaatcg tcaatgccgt caccagcatc aacaacaatt tcacttgctg
     7561 taatttcaca ccatcaacaa acgccagatc cgggctaatc gtcatcgaca gcaaattgcg
     7621 ccattgccag aacaaaatag ccaccacgat gaccacgcca atcgcaatag agatgagatc
     7681 ttctggcgtc actgccagta aatcaccgaa caggtaagcc atcaaatcaa cacgaatgtt
     7741 agacatcaga ctaacgacca ctaggcccag cgacagggca ctgtgcgcca taatccctaa
     7801 taacgtgtcg atcgccagct gtggacgctt ctccagccat accagaccgc ccgccagcag
     7861 cagcgtaacg gcaatcaccg catagaatgg attcacgtcc agcaacaaac caaacgcgac
     7921 accaagtaat gaggcatgag ccagcgtatc accgaaataa gacatacgac gccagactac
     7981 aaacgaaccc agcggacccg cggcacaggc gagcatgatc ccggctaacc aaccgggaaa
     8041 taataattca atcatgagcg atcatttccc cgacgcaaaa caattcgtcc ctgtaaatcg
     8101 tgacgatgat tatgatgatg gcgatagata cccagttgtt cagcaccacg aggaccaaac
     8161 attgaaataa actccggatg cagggaaaca acttccggtg tgccggaaca acaaatgtgg
     8221 tgattcaggc acagcacttc atcggttttt gccatcacca gatgcagatc gtgagaaacc
     8281 attaaaacgc cacaatccag ttcacgacgc aactggtcaa taaggtcata taacgccact
     8341 tggccattta catccacgcc ttgagtgggt tcatccagca ctaataattg cgggcaattt
     8401 aacaatgctc gcgctaatag tacacgctgc gtttcgccac cagagagctt ttgcatcggt
     8461 gcgttaatca ggtgcccggc ctggacacgt ttcagtgcag gcaaaatatc ttctttatgt
     8521 gtaccagggc gtaagcgtaa aaaacggttt acggtcagtg gcaacgtggt gtcgagatac
     8581 agcttctgcg gtacatagcc gatgcgcagt tttccgttgc gcttgataac cccttcatcg
     8641 ggtgttacca gcccgagcac tacccgtacc agtgtcgact tacctgcgcc atttggccca
     8701 agtaaagtca aaatttttcc aggtttaagt tccagcgaca catcagagag gacgcggcgt
     8761 tggccaaaag aaaccgagac attttccagg gaaaccagac ttgtcatgtt aattttagtc
     8821 ttgcagtagt catgaaatgt tataatatca cacttctcat attcattacg atgattggtc
     8881 gcattatgtt acataaaaaa acgcttcttt tcgcagcatt atccgccgct ctctggggag
     8941 gtgcaacaca ggccgcagat gccgccgttg tcgcttcgct taaacccgtt gggttcatcg
     9001 cttctgccat tgctgatggt gtaacagaaa cggaggtttt actgcctgac ggcgcttcag
     9061 aacatgatta ttcactgcgt ccatcggatg taaaacgctt acagaacgcg gacttagtcg
     9121 tttgggttgg cccggagatg gaagcgttta tgcaaaaacc ggtaagcaaa ttaccagaag
     9181 cgaagcaggt aacgattgcg cagcttgaag atgtgaaacc gctactgatg aaaagtattc
     9241 acggcgatga tgatgatcac gaccacgcgg aaaaaagtga cgaagatcac catcacggcg
     9301 atttcaacat gcatctttgg ctttccccag agatagcgcg ggctacagcg gttgcaatcc
     9361 atggaaaatt agtggaactt atgccgcaaa gtcgagccaa acttgacgcc aacctgaagg
     9421 attttgaggc acaattggcc tcaaccgaaa cgcaggttgg taacgagctc gcgccgctca
     9481 aggggaaagg ttatttcgtt tttcacgatg cttacggcta tttcgaaaaa cagttcggac
     9541 tgacaccgct tggtcatttt accgttaacc ctgagattca acctggcgcg cagcgtttac
     9601 atgaaataag aacacagttg gttgagcaaa aagcaacctg cgtttttgct gagccacagt
     9661 tcaggccagc ggtcgttgag agcgtcgcac gagggacatc cgttcgtatg ggaacgttgg
     9721 atcccctggg gacgaatatc aaactgggta aaacaagcta ttcagaattc ctgagtcaat
     9781 tagccaacca gtatgcgagc tgcctgaaag gagattaatg aggaagtgat tacgtgcaac
     9841 agatagcccg ctctgtcgcc ttggcgttta ataatttacc gcgaccacac cgcgttatgt
     9901 tggggtcgct caccgttctt actctggccg tcgctgtctg gcggccctat gtttatcacc
     9961 gtgatgccac gccaattgtc aaaaccattg agctggaaca gaacgaaatt cgttcgctct
    10021 tacctgaagc cagtgagccg attgatcaag ctgcacaaga agatgaagcc attccccagg
    10081 acgaactgga tgacaaaatc gccggtgaag cgggcgtgca tgaatatgtt gtttccactg
    10141 gcgatacgct aagcagcatt ctcaatcagt atggtattga tatgggtgat atcacccaac
    10201 ttgctgctgc cgacaaagaa ttgcgtaacc tgaaaatcgg tcaacaactc tcctggacat
    10261 taaccgcgga cggcgaactg cagcgcctca cctgggaagt gtctcgtcgt gaaacccgaa
    10321 cctatgaccg tactgccgct aacggtttta aaatgaccag cgaaatgcag caaggagagt
    10381 gggttaacaa tctgctgaaa ggtaccgtcg gagggagctt tgttgccagc gccagaaacg
    10441 ccggtttaac cagcgccgaa gtgagcgcag tgattaaagc catgcagtgg caaatggatt
    10501 tccgcaaact gaaaaaaggc gatgaatttg cggtattaat gtctcgagaa atgcttgatg
    10561 gtaaacgtga gcaaagccag ctgctgggcg tacgtttgcg ttcagaaggt aaagattatt
    10621 acgcaatccg cgctgaggat ggcaaattct acgatcgtaa cggtactggt ctggcgaaag
    10681 gattcttgcg attcccgacg gcgaaacagt tccgtatctc gtctaacttt aacccgcgtc
    10741 gaactaatcc ggtgacaggt cgcgttgcac cacacagagg tgttgatttc gccatgccac
    10801 aaggtacgcc agtgctttca gtgggtgacg gtgaagtggt ggttgccaaa cgcagtggcg
    10861 cagcaggtta ttatgtggct attcgtcatg gtcgcagcta caccacgcgt tatatgcact
    10921 tgcgcaagat tctggtgaaa ccgggacaga aggtgaaacg tggcgaccgt atcgcgcttt
    10981 ccggtaatac cggacgttca actgggccgc atctgcacta tgaagtatgg ataaaccagc
    11041 aggccgtaaa cccgctgacg gcaaaactgc cgcgtaccga agggctgacc ggctccgatc
    11101 gtcgcgaatt cctggcgcag gccaaagaga ttgtgccgca gctacggttt gattaattaa
    11161 catccattcg cagccggtac gcagtcagta ccggcttttt ttatttggtg cggggcaagt
    11221 tgtgccgcta cactatcacc agattgattt ttgccttatc cgaaactgga aaagcatgga
    11281 aacgaaaaaa aataatagcg aatacattcc tgagtttgat aaatcctttc gccacccgcg
    11341 ctactgggga gcatggctgg gtgtagcagc gatggcgggt atcgctttaa cgccgccaaa
    11401 gttccgtgat cccattctgg cacggctggg acgttttgcc ggacgactgg gaaaaagctc
    11461 acgccgtcgt gcgttaatca atctgtcgct ctgctttcca gaacgtagtg aagctgaacg
    11521 cgaagcgatt gttgatgaga tgtttgccac cgcgccgcaa gcgatggcaa tgatggctga
    11581 gttggcaata cgcgggccgg agaaaattca gccgcgcgtt ggctggcaag ggctggagat
    11641 catcgaagag atgcggcgta ataacgagaa agttatcttt ctggtgccgc acggttgggc
    11701 cgtcgatatt cctgccatgc tgatggcctc gcaagggcag aaaatggcag cgatgttcca
    11761 taatcagggc aacccggttt ttgattatgt ctggaacacg gtgcgtcgtc gctttggcgg
    11821 tcgtctgcat gcgagaaatg acggtattaa accattcatc cagtcggtac gtcaggggta
    11881 ctggggatat tatttacccg atcaggatca tggcccagag cacagcgaat ttgtggattt
    11941 ctttgccacc tataaagcga cgttgcccgc gattggtcgt ttgatgaaag tatgccgtgc
    12001 gcgcgttgta ccgctgtttc cgatttatga tggcaagacg catcgtctga cgattcaggt
    12061 gcgcccaccg atggatgatc tgttagaggc ggatgatcat acgattgagc ggcggatgaa
    12121 tgaagaagtc gagatttttg ttggtccgcg accagaacaa tacacctgga tactaaaatt
    12181 gctgaaaact cgcaaaccgg gcgaaatcca gccgtataag cgcaaagatc tttatcccat
    12241 caaataaaaa agcctctcgc gaggagaggc cttcgcctga tgataagttc aagtttgctt
    12301 cagaatattc gaaatctgtt gaactatcat tgaactatag gccggatgtg gcgttttcgc
    12361 cgcatccggc aacgtactta ctctaccgtt aaaatacgcg tggtattagt agaacccacg
    12421 gtactcatca cgtcgccttg ggtgacaatc accaggtcac cagacatcaa gtaaccttta
    12481 tcgcgcagca gattaaccgc ttcgctggca gctgctacgc cgtcattagc gctatcaaag
    12541 tgcaccggcg taacgccacg atagagagca gtcaggttca gcgtacgttc atggcgcgac
    12601 atggcgaaaa ttggtagacc agagctgata cgggaggtca tcagcgcggt acgacccgat
    12661 tcggtcatgg taatgatcgc cgtaacgccc ttcaggtggt tagctgcgta cattgccgac
    12721 atggcaatag cttcttccac attgtcgaac tgaacgtcca gacggtgttt agaaacgttg
    12781 atgctcggga ttttttccgc acccaggcaa acgcgcgcca tggctgcaac agtttctgac
    12841 ggatactgcc cagcggcagt ttctgcagac agcatcacag cgtcagtacc atccagaacg
    12901 gcgtttgcta cgtccatgac ttctgcacgc gtcggcatcg ggttagtaat cattgactcc
    12961 atcatctggg tcgccgtgat taccgctcgg tttagctgac gcgcacgacg gatcaacgct
    13021 ttctgaatgc cgaccagttc cgggtcgcca atttccacac cgaggtcgcc acgtgcaacc
    13081 attaccacgt cagaggcgag gatgatgtca tccattgcat cctggctgca aacggcttcc
    13141 gcacgttcaa ccttggcaac aattttcgca tcacatcctg catcgcgtgc caggcgacgg
    13201 gcatagttca gatcttcgcc acagcgtggg aaggagacag ccaggtaatc tacgccaatc
    13261 aacgccgcag tcttaatgtc tgctttgtct ttttcggtca gcgcttcagc cgacaaaccg
    13321 ccgccaagtt tgttgatacc tttattgttg gagaggggac caccgacggt gacttcggtg
    13381 aacactttca tgccctgaac ttccagtact tttaactgga cgcgaccatc gtccagcagc
    13441 aggatgtcac caggcacgac gtcagcaggc aggcctttgt agtcgatacc gactttttct
    13501 ttgtcgcctt cacctttacc caggttggcg tcgagcagga atttatcccc aatattgagg
    13561 aaaactttgc cttctttaaa ggtggataca cggattttgg gcccctggag gtcacccaga
    13621 atagccacat gacgccccag ttttgcggca atctcacgaa ctttatccgc gcgcattttg
    13681 tgatcttcag gcgagccgtg agaaaagttc atacgtacaa cgttggcacc cgccgcgata
    13741 actttttcaa gattattatc gcgatctgtt gctgggccta acgtggtaac gatttttgtt
    13801 ctgcgaagcc ttttggacat gtaatactcc gttgactgaa acaaccaggt gttgcttgaa
    13861 catgaaatcc gaatgaaata acgccgcgat ggcgataaat taatctcatt cggatgtcgt
    13921 ataagattag gacagtgaca gtcgctttta gcgatcgtca cttaaattaa gtaactgctt
    13981 atcaaaacgc gattccttca gcgcttcctt gacccgcttc aagttatctc tgaattttgc
    14041 accgcgtcgc aaagtaaatc ctgtcgccag cacatctatc acggtcagct gtgcaagtcg
    14101 agaaaccatg ggcatataaa tgtcagtatc ttccggtacg tcgagggtaa ttgccagcgt
    14161 tgcttcccgg gcgagcgggg tacccgcaga ggtgagggca atcaccatgg cgtcgttttc
    14221 gcgtgccagc tgcgccagct cgaccagatt ttttgttctt cctgtgtgag aaatcagcac
    14281 caccacgtct ccgtcgctac aattcataca actcatgcgt tgcagcacga tatcatcgga
    14341 gtacaccacc ggaacattaa aacgaaagaa cttattcatc gcatcgtggg caacggcggc
    14401 agaagagcct aatccgaaaa aggcgatttt ttttgcctga gtgagcaagt cgacggcgcg
    14461 gttgatggca gatttatcca gtgaatgacg gacatgatcg agcgttgcca ttgcggactc
    14521 aaatattttc cccgtgtatg attcaacgct gtcatcttca ttgacgttgc gattaacata
    14581 gggagtgcca ttcgccagac tttgtgccag atgaagttta aaatcaggaa aaccgcgcgt
    14641 gtccatgctg cgacagaaac gattcaccgt cggttcgcta acattggctt ccagcgccag
    14701 agcagcaata ctcgaatgga tcgcgttatc gggcgaagcc agaatgacct cggcaacttt
    14761 gcgctctgat ttgctcaaat gttccagctg agactggatt ttttccagca tattcatgat
    14821 gtaaagagac tcacgggtaa tgacgatttc cgcactgaaa gaaatcgaaa tgcagttttg
    14881 tcagatatta cgcctgtgtg ccgtgttaat gacaaaagca gataaaaaag ttgttatttt
    14941 tttcataaca tgatcagtgt cagattttta ccgaatggaa aacgatgatt tttttatcag
    15001 ttttgccgca ctttgcgcgc ttttcccgta atcgcacggg tggataagcg tttacagttt
    15061 tcgcaagctc gtaaaagcag tacagtgcac cgtaagaaaa ttacaagtat accctggctt
    15121 aagtaccggg ttagttaact taaggagaat gacatggcgg taacgcaaac agcccaggcc
    15181 tgtgacctgg tcattttcgg cgcgaaaggc gaccttgcgc gtcgtaaatt gctgccttcc
    15241 ctgtatcaac tggaaaaagt cggtcagctc aacccggaca cccggattat cggcgtaggg
    15301 cgtgctgact gggataaagc ggcatatacc aaagttgtcc gcgaggcgct cgaaactttc
    15361 atgaaagaaa ccattgatga aggtttatgg gacaccctga gtgcacgtct ggatttttgt
    15421 aatctcgatg tcaatgacac tgctgcattc aaccgtctcg gcgcgatgct ggatcaaaaa
    15481 aatcgtatca ctattaacta ctttgccatg ccgcccagca cttttggcgc aatttgcaaa
    15541 gggcttggcg aggcaaaact gaatgctaaa ccggcacgcg tagtcatgga gaaaccgctg
    15601 gggacgtcgc tggcgacctc gcaggaaatc aatgatcagg ttggcgaata cttcgaggag
    15661 tgccaggttt accgtatcga ccactatctt ggtaaagaaa cggtgctgaa cctgttggcg
    15721 ctgcgttttg ctaactccct gtttgtgaat aactgggaca atcgcaccat tgatcatgtt
    15781 gagattaccg tggcagaaga agtggggatc gaagggcgct ggggctattt tgataaagcc
    15841 ggtcagatgc gtgacatgat tcagaaccac ctgctacaaa ttctctgcat gattgcgatg
    15901 tctccgccat ctgacctgag cgcagacagc atccgcgatg aaaaagtgaa agtactgaag
    15961 tccctgcgcc gcatcgaccg ctccaacgta cgcgaaaaaa ccgtacgcgg gcaatatact
    16021 gcgggcttcg cccagggcaa aaaagtgccg ggatatctgg aagaagaggg cgcgaacaaa
    16081 agcagcaata cagaaacctt cgtggcgatc cgcgtcgaca ttgataactg gcgctgggcc
    16141 ggtgtgccat tctacctgcg tactggtaaa cgtctgccga ccaaatgttc tgaagtcgtg
    16201 gtctatttca aaacacctga actgaatctg tttaaagagt cgtggcagga tctgccgcag
    16261 aataaactga ctatccgtct gcaacctgat gaaggcgtgg atatccaggt actgaataaa
    16321 gttcctggcc ttgaccacaa acataacctg caaatcacca agctggatct gagctattca
    16381 gaaaccttta atcagacgca tctggcggat gcctatgaac gtttgctgct ggaaaccatg
    16441 cgtggtattc aggcactgtt tgtacgtcgc gacgaagtgg aagaagcctg gaaatgggta
    16501 gactccatta ctgaggcgtg ggcgatggac aatgatgcgc cgaaaccgta tcaggccgga
    16561 acctggggac ccgttgcctc ggtggcgatg attacccgtg atggtcgttc ctggaatgag
    16621 tttgagtaat atcagcgctt atcctttatg gttattttac cggtaacatg atcttgcaca
    16681 gattgtagaa caatttttac actttcaggc ctcgtgcgga ttcacccacg aggctttttt
    16741 tattacactg actgaaacgt ttttgcccta tgagctccgg ttacaggcgt ttcactcata
    16801 aatcctctga atgaaacgcg ttgtgaatca tcctgctctg acaactcaat ttcaggagcc
    16861 tttatgaatc cacaattgtt acgcgtaaca aatcgaatca ttgaacgttc gcgcgagact
    16921 cgctctgctt atctcgcccg gatagaacaa gcgaaaacgt cgaccgttca tcgttcgcag
    16981 ttggcatgcg gtaacctggc acacggtttc gctgcctgcc agccagaaga caaagcctct
    17041 ttgaaaagca tgttgcgtaa caatatcgcc atcatcacct cctataacga catgctctcc
    17101 gcgcaccagc cttatgaaca ctatccagaa atcattcgta aagccctgca tgaagcgaat
    17161 gcggttggtc aggttgcggg cggtgttccg gcgatgtgtg atggtgtcac ccaggggcag
    17221 gatggaatgg aattgtcgct gctaagccgc gaagtgatag cgatgtctgc ggcggtgggg
    17281 ctgtcccata acatgtttga tggtgctctg ttcctcggtg tgtgcgacaa gattgtcccg
    17341 ggtctgacga tggcagccct gtcgtttggt catttgcctg cggtgtttgt gccgtctgga
    17401 ccgatggcaa gcggtttgcc aaataaagaa aaagtgcgta ttcgccagct ttatgccgaa
    17461 ggtaaagtgg accgcatg
//
LOCUS       DAECNO010000086        17173 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.086, whole genome shotgun sequence.
ACCESSION   DAECNO010000086 DAECNO010000000
VERSION     DAECNO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.086"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..185
                     /locus_tag="KKO08_002975"
     CDS             <1..185
                     /locus_tag="KKO08_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBD6319143.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..401)
                     /locus_tag="KKO08_002976"
     CDS             complement(210..401)
                     /locus_tag="KKO08_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBD6319144.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="KKO08_002977"
     CDS             complement(506..742)
                     /locus_tag="KKO08_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBD6319145.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KKO08_002978"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="KKO08_002978"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBD6319146.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KKO08_002979"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="KKO08_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KKO08_002980"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="KKO08_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBD6319147.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /gene="msrC"
                     /locus_tag="KKO08_002981"
     CDS             6381..6878
                     /gene="msrC"
                     /locus_tag="KKO08_002981"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBD6319148.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="KKO08_002982"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="KKO08_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBD6319149.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="KKO08_002983"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="KKO08_002983"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBD6319150.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="KKO08_002984"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="KKO08_002984"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBD6319151.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="KKO08_002985"
     CDS             complement(10860..12233)
                     /locus_tag="KKO08_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6319152.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="KKO08_002986"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="KKO08_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBD6319153.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="KKO08_002987"
     CDS             complement(13344..13583)
                     /locus_tag="KKO08_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319154.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="KKO08_002988"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="KKO08_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBD6319155.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="KKO08_002989"
     CDS             13960..14247
                     /locus_tag="KKO08_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBD6319156.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="KKO08_002990"
     CDS             14826..14969
                     /locus_tag="KKO08_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBD6319157.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="KKO08_002991"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="KKO08_002991"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBD6319158.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="KKO08_002992"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="KKO08_002992"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBD6319159.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KKO08_002993"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="KKO08_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBD6319160.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4155 c         4337 g         4149 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DAECNO010000087        16388 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.087, whole genome shotgun sequence.
ACCESSION   DAECNO010000087 DAECNO010000000
VERSION     DAECNO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.087"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="KKO08_002994"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="KKO08_002994"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBD6319161.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KKO08_002995"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="KKO08_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBD6319162.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="KKO08_002996"
     CDS             2337..2552
                     /locus_tag="KKO08_002996"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319163.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="KKO08_002997"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="KKO08_002997"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBD6319164.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="KKO08_002998"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="KKO08_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBD6319165.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="KKO08_002999"
     CDS             5918..6415
                     /locus_tag="KKO08_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBD6319166.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="KKO08_003000"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="KKO08_003000"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBD6319167.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="KKO08_003001"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="KKO08_003001"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBD6319168.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="KKO08_003002"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="KKO08_003002"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBD6319169.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="KKO08_003003"
     CDS             9100..9663
                     /locus_tag="KKO08_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBD6319170.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="KKO08_003004"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="KKO08_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBD6319171.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="KKO08_003005"
     CDS             complement(10255..11235)
                     /locus_tag="KKO08_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBD6319172.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="KKO08_003006"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="KKO08_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBD6319173.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="KKO08_003007"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="KKO08_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBD6319174.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="KKO08_003008"
     CDS             12538..12828
                     /locus_tag="KKO08_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBD6319175.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="KKO08_003009"
     CDS             12836..13429
                     /locus_tag="KKO08_003009"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBD6319176.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="KKO08_003010"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="KKO08_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBD6319177.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="KKO08_003011"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="KKO08_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..16383
                     /locus_tag="KKO08_003012"
     CDS             15880..16383
                     /locus_tag="KKO08_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBD6319178.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4058 a         4220 c         4126 g         3984 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aatcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtggc gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattaac
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgtgtt ttattaatca aagcattcaa caactgaaac aacctgcgca ggaggccagt
    16381 taatggat
//
LOCUS       DAECNO010000088        16102 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.088, whole genome shotgun sequence.
ACCESSION   DAECNO010000088 DAECNO010000000
VERSION     DAECNO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.088"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(176..1072)
                     /gene="yhaJ"
                     /locus_tag="KKO08_003013"
     CDS             complement(176..1072)
                     /gene="yhaJ"
                     /locus_tag="KKO08_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBD6319179.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1177..1878
                     /locus_tag="KKO08_003014"
     CDS             1177..1878
                     /locus_tag="KKO08_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBD6319180.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1901..2065
                     /locus_tag="KKO08_003015"
     CDS             1901..2065
                     /locus_tag="KKO08_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319181.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2277..3587)
                     /locus_tag="KKO08_003016"
     CDS             complement(2277..3587)
                     /locus_tag="KKO08_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBD6319182.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3615..4946)
                     /locus_tag="KKO08_003017"
     CDS             complement(3615..4946)
                     /locus_tag="KKO08_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBD6319183.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5221..6585)
                     /gene="tdcG"
                     /locus_tag="KKO08_003018"
     CDS             complement(5221..6585)
                     /gene="tdcG"
                     /locus_tag="KKO08_003018"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBD6319184.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6657..7046)
                     /locus_tag="KKO08_003019"
     CDS             complement(6657..7046)
                     /locus_tag="KKO08_003019"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBD6319185.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7060..9354)
                     /gene="tdcE"
                     /locus_tag="KKO08_003020"
     CDS             complement(7060..9354)
                     /gene="tdcE"
                     /locus_tag="KKO08_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBD6319186.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9388..10596)
                     /gene="tdcD"
                     /locus_tag="KKO08_003021"
     CDS             complement(9388..10596)
                     /gene="tdcD"
                     /locus_tag="KKO08_003021"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBD6319187.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10622..11953)
                     /gene="tdcC"
                     /locus_tag="KKO08_003022"
     CDS             complement(10622..11953)
                     /gene="tdcC"
                     /locus_tag="KKO08_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBD6319188.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11975..12964)
                     /gene="tdcB"
                     /locus_tag="KKO08_003023"
     CDS             complement(11975..12964)
                     /gene="tdcB"
                     /locus_tag="KKO08_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBD6319189.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13063..14001)
                     /gene="tdcA"
                     /locus_tag="KKO08_003024"
     CDS             complement(13063..14001)
                     /gene="tdcA"
                     /locus_tag="KKO08_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBD6319190.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14191..14535
                     /gene="tdcR"
                     /locus_tag="KKO08_003025"
     CDS             14191..14535
                     /gene="tdcR"
                     /locus_tag="KKO08_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBD6319191.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14791..15330
                     /locus_tag="KKO08_003026"
     CDS             14791..15330
                     /locus_tag="KKO08_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319192.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15352..15699
                     /locus_tag="KKO08_003027"
     CDS             15352..15699
                     /locus_tag="KKO08_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319193.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15868..>16102
                     /locus_tag="KKO08_003028"
     CDS             15868..>16102
                     /locus_tag="KKO08_003028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319194.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLK"
BASE COUNT         4364 a         4155 c         3627 g         3956 t
ORIGIN      
        1 ttgttgacaa agtgcgcttt gtttatgccg gatgcggcgt aaacgcctta tccagcctac
       61 aaattgccaa aattcaatat attgcagtga ctgcgtaggc ctgataagcg tagcgcatca
      121 ggcagttttg cgtttgtcag cagtcagaag caccttgtga aaggtgctta atagattatt
      181 ttccagcgaa caactttgga atttcccgca gacaccagga tttcgcttcc cccatactgt
      241 cacgacgcca ggccataata atatcgatct cgctggtcga ttccgggctg acgacacgca
      301 accgcccttc cgcaatatct ttttcgacca tcggatacgg catcgtcgcc acgccaagcc
      361 ccgccagtaa tgcctgacgt ttatcttcaa tcgtgctcac cgttaagcgc ggctgtttgt
      421 ccagcagctg tacggtcaac accgggcgct cacgagcggt atccgctacc gcaattccac
      481 gatatttcac acgcgtcact tcagataacg gttccggctc ctgatgaatc gggtgatcag
      541 gcgcggcaac gtagacgttc attaacgtat agagcttgcg cgagttgatc tccgacgagg
      601 aacgaaaatg catatccggc gcgataacga tatccgcccg cccctgctcc agccgttccc
      661 acgcccccgc cagcacttct gtgatgattg ccagttgggt attggctttt gccgccagtt
      721 tgtcgattaa cgggaaaaag gcaggtgtcg gtaccagcgc ttcggtcaca atggtgagat
      781 gcgtttccca accgcgcgcg agagcttccg catcagtagt cagtttatct gcggcttcca
      841 gcaaaacgcg ccctcgctcc agcaacatcc tcccgacatt ggtgaatttg gtgcgatggc
      901 ccgagcggtc aaacagcacc acatccagct cttcttccag tttttgcatg gtgtagctaa
      961 gtgcggaagg cacgcgtccc agctcatccg ctgccgccgc aaaactgccc cggcgatcga
     1021 tcgcatccat aacccgtagt gcttccagcg ttaatgccct ttctttggcc atttcgttct
     1081 cattcaggaa atttgaacat accgggcaga atatctggct aacaatgtag cgtccagccc
     1141 cttaccataa aaggaagtaa agagaggtca agaattatga ttactacccg aactgccagg
     1201 cagtgtggac aagcagacta cggatggttg caggcccggt atactttttc ctttggacac
     1261 tacttcgacc cgaaattgtt aggctatgcc tccctgcgtg tgcttaacca ggaagtgctg
     1321 gccccaggtg ccgcctttca gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt
     1381 ctggatggag aagcagagta tcgcgacagc gaaggcaatc atgttcaggc cagcgccgga
     1441 gaggcgttgc tgctctctac ccagccgggt gtcagctata gcgaacacaa tctcagcaaa
     1501 gacaaaccgt taacgcgaat gcagctttgg ctggatacct gcccgcagcg agagaatccg
     1561 ctgattcaaa agctggcgct taatatggac aagcagcaat taatcgcctc gccagagggt
     1621 gcgatgggaa gcctgcaatt acgccaacaa gtgtggctgc accatatcgt gctcgacaaa
     1681 ggcgaaagtg cgaatttcca gttgcatggg ccacgcgcgt atttgcaatc gattcacggg
     1741 aaatttcatg cgcttacgca tcatgaagag aaagcggcgc taacctgcgg tgatggggcg
     1801 tttattcgtg acgaggctaa cattacgctg gttgccgatt ccccactgcg cgctttgctg
     1861 atagatttgc ctgtctagtt gtttttacag ggagatgatg atgagtaaaa aatcgaccaa
     1921 aaagcgccag ccagcgaagc ccgtggtggc gaaagaacct gctcgcaccg ccaaaaattt
     1981 tggctatgaa gagatgttga gcgagctgga agctatcgtc gcggatgctg aaacgcgttt
     2041 agccgaggat gaagctaccg cgtaaagaca ggttgccgga tgcggcgtaa acgccttatc
     2101 cggcctacaa aacacaacaa catcaacaaa ttaaacaccc cgtaggccgg ataagatgcg
     2161 ccagcatcgt atccggcatc aggtacaaaa cacaacaaca tcaacaagtt gagcaccccg
     2221 taggccggat aaggtgcgcc agcatcgcat ccggcacaat actcaaaacc tggcgtttat
     2281 ctggccttgc tcgccataat ctcgataatc tgccgatccg tttgctgcat cgaatggctt
     2341 gctaacgcac acaggttggc aatcgactgc tcaacatcat gcgccacgat cccttcattg
     2401 ccggtcacgg cggtatcatc cagcgccatt aacaccgctt tccacgcagc cgaagcactg
     2461 gtcgaaacct tcatcgcgca gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg
     2521 ccgatcatac tgctgatcgc catcgagata gtttcataac gcccatccac cagccatgcc
     2581 atcccggcgg cggcccccat tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt
     2641 aactggttat ggatgtaaat tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt
     2701 tcatcatccg ctccgaagtg ttctgctaca accaccacag gcattattgc agtgatcccc
     2761 tggttacccg agccggagtt actcatagcc ggaagcgtag cgccgcccat acgcgcatcg
     2821 gatgccgcgc tggtacgaat cacaatggat gaagagagat ctttcgccag caaaccgcgc
     2881 tcgcactgtt tttccagcgt cgcgccaata tgcagccccc acttaccgct caaaccttcc
     2941 tgcgataacg cacaatttag cttcgcggaa tcgagaataa agcggatcgc cgcaaatggg
     3001 acttcattga cgaacttcag gatctcagcc agcgtcgttc tggaaagcac cgtcagcggc
     3061 gactcttgct cgccctctgc cacacacgcc tggtgggtaa acaccacacc attgtgcgtc
     3121 tcaatatgca caatgttggt atgcccgccg acgatggtga cacacgccca cttctcaccg
     3181 ttccagactt tggcgcgtga gaagaggatt tcattgcaag gttcctggat cttaacagag
     3241 actttccccg ccgccagcag tgctttggca tcggcaattg cctgcgcagt tgcgtctttc
     3301 agcacttcca gcccggcgtt ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc
     3361 agccccacca ttcccgtgcc gggaacggtg acgcccagac cgttcttcat cagatttggc
     3421 gaaacccagg cttctacacg ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc
     3481 gccagcgcca gtgaaatcgg ttcagtacat cccagcgccg gttttacttc ctcctgaacg
     3541 gcgaggatgt aacgctgcca taacggattt aaagtcgaat caaacatatt aaaaacctta
     3601 aaatttcagg taaatcaaga aaatgccagg aacagagaaa cacaaagcaa caaaccagtg
     3661 acgataatca ggtacagaga catccctttg tatttgtgca atgccggtac tttgtaaacc
     3721 agccacgccg ggatcaggca ccctaccatg ccgaaaatcg ggctacagat agaggtgaag
     3781 ctcaacaccg gtgcgttcag tacgatggcg ctccaggcca gcaaaatggc gaaaatcatg
     3841 atgccgcgct gaacaagatt ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg
     3901 ttcattacga tcccttgcgt tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc
     3961 atgacagcaa agatattgag aatgacgctg accactttca cccacgctgc gccgtcaccg
     4021 ctaataaact gtgcggcaat cgccagcgcg gaaatattct gctcgtaggc ttttaccgct
     4081 tcgtcatgtc ccatcgccag cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa
     4141 atgccaaacg cgatattcat tgcccgcaat gctttatgcc gcgccacttc aattgatttt
     4201 tcccgcgagc gataagagat caccatcggg cttaacgtct ggataaacag aatcgacgtc
     4261 agggtaaacg gcagcgtaat aatggcgttt ttcaccagca gccccagcgg cggtagcgaa
     4321 ccgacgttat acagatgcca cattcccacc atcgacacgc ccagcgcagc gactaccagc
     4381 agcttggtca gcaccatgtc ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag
     4441 atcgccacca gaatgcaaat cagtaccaga ccataaaagg gactgtctga cagcaaccct
     4501 tccgtcacgc cgaaggtatg cagataggaa gcactatcgt tggtgatggc ggtggaatag
     4561 acgaacatcc aaatcaccag cattacgaaa tagagcgcac ctaacaggat gccccagttt
     4621 ttacctaaat aaccgctaat gacactcggg taatctttac attctggtga ttctgccagc
     4681 gtattaataa acaaccgctg aaacagatac attgccgggt aaccaatcac cgatgagagc
     4741 aaaaataccc acaatcccat caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca
     4801 atcgccatcc cgatactcat aatcacccag ccggtgtcag tactgtcgaa tttgatcgct
     4861 tctcgccact cgctctcact cattccagca cgacccgccg gggtcgaagc gtctgcaatg
     4921 acgcctttat tcgatgcaat ttccataatt tctcgctcaa tattttgtag ggcttattat
     4981 ttttttccga gccgcatcaa ggcgatatgc ggtctacgtt tttgcaggcg agttattaga
     5041 ataaagaaat gatacgcgcg aggtcagaga aaatcttcac aatcaagact ttaaaataac
     5101 ggagatggat aagaattttc tactaaatta atcgcaagag aatattcatt ctctatttgc
     5161 ggcagatcac aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct gacagcaatg
     5221 tcagccgcag accactttaa tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt
     5281 catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc
     5341 cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat taatggcatt
     5401 acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt
     5461 atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccgc ccagtagttc
     5521 agttaaccct gccgctgcca ttgaacaggc cacgccaatc tccccctgac agccgacctc
     5581 cgcgccagag atggaggcgt tcattttata cagcgcgcca atagcccccg ctgccagaaa
     5641 atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat aagccagcac
     5701 tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc cgccagctgc
     5761 gttttcttca ctaaccgcca gcgcgtacat gttgatccag tcgatgacat tcatcggatc
     5821 gttagagatg ttatcgctag aaaccagctg acgacgcagc gctacggcac gacacggcac
     5881 attgagcgga ccaggcagca cgccttcagt gttcatcccg cgttcaatac cgtcatgcat
     5941 cacttgccag atacgggcaa accctgcgtc aatttccgct ttgctgcgca gcgccagctc
     6001 gttgtgcatc atcaggccag atatagacag gccgttgtaa tcacacattt tcagcagttc
     6061 acctgctgag tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg
     6121 ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttactta atagcgcttc
     6181 ctgtcccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca tttcgggatg
     6241 gaagataatg ttctttgcta caggaaaatc aacaatatgc gcaccagatg ccactggcag
     6301 ccgcccgctg cgcgttacta actctataaa tgcagggatc tcatcaatga caacatcctg
     6361 cggactgttt cctgccagtc ccatgataat ggcgacatcc gtggcatggc ctttgcccgt
     6421 cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta ataagccgct
     6481 actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacgg tatgcgaact
     6541 ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca caccctcgga
     6601 ttgccgttca gtgaagtgga gcggaacgac cttgcgaccg tcccgctcac gaggctttac
     6661 gcactacgta ctgcgatagc ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc
     6721 tgcacacagc tccgggtcgg gtaggtcgcc tgatgctcat cgaagaactg cttatagact
     6781 tcattgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt gatgatatcg
     6841 cccacgctca gcccggcagc aaccacgatc gctttgacgt tttcgaggct taaacgtgcc
     6901 tgatcttgca catcagccgg gatctcaccg gtctgcgggc aaaccggaat ttgcccggag
     6961 gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc gcctggggca
     7021 cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct gggtaaaagt
     7081 acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc
     7141 agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat ccagcagcat
     7201 ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg
     7261 gaaataccca tccagcaggc caacaaggtt ggttttacgt actggatctt ctttgcccag
     7321 cgccgcagga acgattgaga aggtgtacga gatcccatct ttggcgtagg tgaacggcag
     7381 tttcgccacc gacgtcaatg aggccacggc acctttgcgg tcacgaccat gcatcgggtt
     7441 agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc ggcgtattac cggttttctg
     7501 gccgtacacc acgttagaag tgatagtcag aatcgactgg gtagggacgg cgttgcgata
     7561 ggttggcagc gctttaattt tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc
     7621 tacgcgctcg tcgttgttgc cgtactgcgg atattcaccg tcgatttcaa agtccaccgc
     7681 caggccgttt tcgtcacgga ttggtttcac gcgggcatat ttgatggcag acagggagtc
     7741 cgtcgccacc gacaggcccg cgatgccgca tgccatagtg cgatagacat cacgatcgtg
     7801 cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag
     7861 cgcgctgatg tactgcaccg ccagccagtc catgaagtga tcgaggctgt ccatcacttt
     7921 gtcgtaatcc agcacgtcgt ccatcagcgg tgctgttttc ggcccgacct gaatcttcag
     7981 cttctcgtcc accccgccgt taattgcgta gagcagcgtt ttcgccaggt tagcgcgtgc
     8041 accaaagaac tgcatttgct taccaatcac cattgggctg acgcagcagg caatcgcgta
     8101 atcgtcgctg ttgaagtcag tacgcatcag atcgtcattt tcatactgca aggaagaggt
     8161 gacgatcgac acctgcgcgg catatttttt gaaggcaatc ggtaattctt ccgaccaaag
     8221 aatggtcagg ttaggttccg gtgccggccc catagtgtgc agggtgtgca aatagcggaa
     8281 ggagtttttg gtcaccagcg tacgaccgtc cagccccatc ccgccgatca cttccgtcgc
     8341 ccagattggg tcgccggaga acagcgaatc aaattccggt gtacgcagga agcgtaccat
     8401 acggatcttc atgatgaagt gatcgatcag ttcctgtgcc tgctgctcat tgaggacgcc
     8461 agctttaaag tcgcgctcaa tgtagatatc caggaacgat gccgtgcgcc ccagcgacat
     8521 cgcgccgcca ttttgcgatt tcactgccgc cagataagcg aagtagagcc actgcaccgc
     8581 ttcctgtgca ttctgcgccg ggcgagagat atcaaagcca tatttcgccg ccatttcctg
     8641 aatctgcaac agcgcatgac gatgctctgc cagctcctca cgcagacgga tggtggcttc
     8701 cagatcctcg cctttttcca gacgagactg gagatcggca aattgcagtt cgcgttcacg
     8761 caccagataa ctgatgccat acagcgctac gcggcgatag tcaccgataa tgcgcccacg
     8821 accatagcca tctggtaaac cggtcagcac gccagattta cggcagcgca gcatatccgg
     8881 tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa acagatattc
     8941 aaattcactg tccatttcac ggccataggc gtggaatgaa cttttaatca tattaatgcc
     9001 accgaacggg tgtagcgcac gtttcaacgg cgcatccgtt tgcaggccaa caattttttc
     9061 cagcggctgg ttaatatatc ccgcatcatg agcggtaatt gtggtggcaa tattggtatc
     9121 gaaatcaacc ggcgcgtggg ttgcgttttc gatacggatg ccttccatta ccttttccca
     9181 caattccgtg gtggcaggcg tcgcttcggc gagaaaagat tcatcgcctt catacggtgt
     9241 atagttatgt tgaataaaat cgcggacatt aatttcgctt ttccagtccg tacctttaaa
     9301 gccaagccat gcgtcagcgt acagcttatc gctggtatca atatctacct tcatgaaaaa
     9361 taatctctct acaatacttc aactaaatta tgcaaattct gcgggcgcgt taactttacc
     9421 taaatgaatg gcatccaaag caatcatttt ttcttcgtta gtcggaataa cgacgcaaat
     9481 gacatgcgca ttttcactgg aaacaattcg ctcgccaaaa gagttagatc gattattcat
     9541 ttctgtatca atctctacgc ctaatacagc caaatgttcc atgaccagac gacgaattaa
     9601 acttgaattc tctcctattc cgccggtgaa tataattcca tccagccgac gtaatgaagc
     9661 tgcatgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg ccagttgcgc
     9721 gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag aaagaccgga
     9781 aatacctaat aatcccgact ctttattcac tacgcgttcc aggtcaccca ggctctggtt
     9841 ggtttggctg gcgacccagg acatcgcacc aaagtcgaca tcgccactgc gggtacccat
     9901 catcaagcct tccagcggcg tcattcccat tgaggtgtca acactctgac cgttgcgaac
     9961 tgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt catcttccgc
    10021 cagattcagc agcgaatgtg cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc
    10081 ataacggcgt acacctaact cttcataata tttccacggc aggccgtata aataagcttc
    10141 cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta cgcccggaaa
    10201 taattgctgc gccgattcaa taccacttaa atttgcgtaa ttatgcaggg gtgccagtgg
    10261 agaaacgcga cggatattat caatgacttc atcggtaata atggcggact cggtaaaaat
    10321 actgccgccg tgagcgatgc ggtggccaat taaggccacg ctgtcattta aattccgttt
    10381 ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc
    10441 tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt cggcaatacc
    10501 tgacattaat acttcacaat cgctggcatc gagtacggaa aacttaatcg aagacgaacc
    10561 acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc tgagttacgg
    10621 attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg gtaacaaaca
    10681 cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggata gcatacatcg
    10741 gcaacaggca cagcagggat gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga
    10801 tgttcgggtt ggcgtaggca acaacccagg tggagcccat gatgaagatc atgctgagag
    10861 tattcagttt acccagcgac actttggttt tgtcaccttt ataaccgaac ttcagaatca
    10921 gaccattcaa gccttccagc gtccccagat agtgaccgaa gaaagatttg aagatagcca
    10981 cgagtgcgat gatggaagcc gcatattcca gtgtaatcgc gaacgttgtt ttcgtaccgg
    11041 tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc gctttggctt
    11101 ccgccatgtt ggccggagac agagtaaaca ggcagctaaa ggcaaagaac atcaccactg
    11161 caaccatcag catgctggca cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc
    11221 gaccgaagtc tttctcatac tcttcacgtt tagaaaccac gaaggaagag acgattggcg
    11281 agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacagtgatc aggataccgt
    11341 catgaccggt taacgacagc gaaccgaggt caacctggtc gataactgca gagttccagt
    11401 aagggatcag cgacagagaa atcagcacca ggctggcgat aaacggccat accaggtagc
    11461 tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc agcagcaaca
    11521 ggaacagcgc cacaaagccg cgattcagcg gtgcaaatcc gagctggttt tcccagaacg
    11581 tcataaaggt attggtaata gtaacgccat aaatccacag cagtgggcaa atcgcgaaga
    11641 agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc accgtttccg
    11701 taatgttgcc ggaagggtta gagccagaaa gacacagacg cgccagcgcc cggtggcaat
    11761 aaaatgcgat ggggtatgcc aataccaaca tcagaagaat cgggatcagt ccgccaaaac
    11821 ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac aagcctaacg
    11881 tccatgtggt atctgattta cgccaggacg attgttttgt ctggctggat acaatgctat
    11941 ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt
    12001 gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt tttctgtttt
    12061 gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg cctgcgcctt
    12121 cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt tcgtcttcgc
    12181 tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa ttacccgggc
    12241 gggagacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtggtt atttctccgg
    12301 agtggaaaga agccgccatg ccgtgaacgt tttctgactg tacgccaata acacgaatgg
    12361 tcgggttaat agatttaatt gccaccgcaa taccagcaat taaaccacca ccaccaattg
    12421 gcacaatcac gttatcgaca tcatagagat cttccataat ttccagacca atcgttccct
    12481 ggccagcaat cactttcgga tcatcgtaag gtgggataaa aatacgacct tccatttcga
    12541 caatttcgct cactttagcg atagtgtcgt tgaagttatc accatgcaga acgacttctg
    12601 cggagtagtc gcacgttgcc gctactttgg attttggcgc accttttggc atcaccactt
    12661 taccgtcgat acccagcatc gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag
    12721 aacaggccac cacgcctttg cgtttttccg catcggtcag tgaacttaat ttattaaatg
    12781 cgccacgaat tttaaatgaa cccgtacgct gcatattttc aaacttcagg aatatttcac
    12841 ctttgcaacg ttcactaaaa tagttggagc gaggcatgcc tgttttataa attcgcccag
    12901 ccagtcgttg tttcgcttca ataatatcat caatagcaac cggcagatcg tatgtaatat
    12961 gcattataaa acctcttcgc ctgaattaaa tatgtaggta accgtaaccg acacctgcaa
    13021 aacagacagg tggattattt atgtttaaat aaaaacaaac aactaaccaa cttctattaa
    13081 ttgccttcgt ctacacccat tataagatga atactctttg gctaattcca ccaaaaccga
    13141 tgctgctttt ttaatacgat aatttttcga ccataccgcg gcatattgtg ccacaggtaa
    13201 tgtttcttca accggaatag taataaattg attagaacca aaaggtgacg tcatatcaca
    13261 aggaattaca gttaagaaat cagcattgag aacaagatta taaattgtca cgactgagtc
    13321 ggttttaacg atgttttcaa tactgatgcc atttctttgt aacgtagtaa gcagttcgct
    13381 gtagtacccc atattagttt gtggcaacac ccactgttcg ttcttcaacg actccagcgt
    13441 ggtggtgccg gtgcatgttc gggacttact ggctaccagc acaaactcgg actcgaacag
    13501 cggctcaaca tgtaaatcct gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc
    13561 cagtcgaccg tcgcggattg ccggtaagaa cgaagacagt tgcgcttcat acatagaaac
    13621 ctgcgctttc gggaacacct ctttgaactt gttgatcatc cctgacataa aagtaaaacc
    13681 aatcaatgaa ggaaaaccaa atgagacttc caccaccgct tcagaagaca taccgcttat
    13741 ctcattaacc atatttttca tttcacgggt aatggattcg gaacgggaga gtaacaattg
    13801 accggcaggt gttaatgtta caccggtatt tttccgcacc actaattcca caccaaaata
    13861 atcttcaata tcgttaatga ttttactgac ggccggttga gttaacccta attcttttgc
    13921 agccgagccg atagagccac ttctaatgac ttcctgaaag actaccaggt gctgcgtttt
    13981 cggaagaaga atagtgctca taacgaccta cgttaattac ctcattgacg gcatgaagtg
    14041 tatcaaaatg aaataaacag gatatgtgcg accactcaca aattaacttt cattactttc
    14101 caaggtatcg ttattacaat taaatatctt taaatatcaa caagttaaag cataaaaatc
    14161 agcataaacc ctgattttta tcaaaaaaaa atgacgggga taaccatatt ttatggcgat
    14221 aacatcattc gttatgtggt taatacaaaa aaggggctga gaccatattt caagcaatta
    14281 ccggataatt accaggcaaa atttgagtta aatttaatga gcaagttctc aaattttatt
    14341 ataaataaac cattttcagc gataaatact gcggcacgtc acattttttc acgctatttg
    14401 ttggagaaca aacatttatt ttatcaatat tttaaaattt cgaatacagg tattgatcat
    14461 ctcgaacaat tgattaacgt caactttttc tcttctgaca ggacgtcatt ttgtgaatgc
    14521 aatcgttttc cataaattct tctcccctca taggcgacga atagcatttt gtgttgagga
    14581 tcacaaaacg aataattgct ggtcgccgcg ataaggtcag acgaagtcaa caagggggat
    14641 tttcactgtg ctttacatca gcgtaggaca cagaatgttg cttcgtttac tgtaacgccg
    14701 ggtaaatgag cgttttttga tagtgcgtaa gtgtcgtgcg aggaaaataa gcatctatta
    14761 ttgtttgtca tgacgacaag gcaggcatca atgaaaggct tcccaattgc acatattttt
    14821 cacccttcaa tcccgccaat gcacgcagtg gttaacaatc acaatagaaa tattgattat
    14881 tggacggtaa aaagaaagtt tgcagaaatt gtctccacca atgacgttaa taaaatttac
    14941 agtataagta atgaactccg gagagtatta tctgcaatta ctgcattgaa tttctatcaa
    15001 ggcgatgttc cttctgtcat gatccgaatc caaccggaaa atatgagtcc attcatcata
    15061 gatatttcta caggagaaca tgatgattat atcatacaaa cattagatgt aggcactttt
    15121 gcaccttttg gtgaacaatg tacttgctca gccgtcaata aaaaagagct ggaatgtatt
    15181 aaggagacga tttctaagta ttgcgcaaag ttcacccaaa aagaagccat cttaacaccc
    15241 cctaggcact ttaataaaac ctgcattact tcagattgtt ggcaaattct ctttttttca
    15301 cccgatcatt ttaataatga tttttattga ttatttataa aggataaaat aatgttccct
    15361 gtttcatcaa ttggtaatga tattagtagt gatttagtcc gtcgaaaaat gaatgacctt
    15421 cctgaaagcc ccataggaaa taacctcgaa gcactagcac caggtataga aaaactaaaa
    15481 cagacctcta ttcaaatggt cactttactt aacgcgttac aaccaagtgg aaaatgcatt
    15541 atcactggtg attttcaaaa agaattagcg tacttacaaa atgtaattct ttataatgac
    15601 tcgtctcttc gtctggattt tttaggttat aacacccaaa ttattcaacg atcggacaat
    15661 acctgcgaac ttaccattaa tgaaccgtta aaaaactagg aaatatctac aggcaatatc
    15721 aatgttaatt gcccattaaa agatatttac aatgaaatca ggaggttgaa cgtaatcttt
    15781 agttgtggga ctggagatat agttgatcta tcctctctgg acttacgtaa tgtcgattta
    15841 gattattatg atttcacaga taaacatatg gctaatacta ttctaaatca ttttaaattg
    15901 gattcaacaa attttactaa tgccaacatg tttcaggtta attttgttag ttcaacacaa
    15961 aacgccacaa tctcctggga ttatttacta aaaataacgc ctgttttaat aagcattagc
    16021 gatatgtatt ctgaagaaaa aatcaagttt gtcgaaagtt gtttaaatga gcttggagac
    16081 attaccgaag aacaattaaa aa
//
LOCUS       DAECNO010000089        15799 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.089, whole genome shotgun sequence.
ACCESSION   DAECNO010000089 DAECNO010000000
VERSION     DAECNO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.089"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KKO08_003029"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="KKO08_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBD6319195.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="KKO08_003030"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="KKO08_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBD6319196.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    546..669
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="KKO08_003031"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="KKO08_003031"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBD6319197.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="KKO08_003032"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="KKO08_003032"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBD6319198.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="KKO08_003033"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="KKO08_003033"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBD6319199.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="KKO08_003034"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="KKO08_003034"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBD6319200.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="KKO08_003035"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="KKO08_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBD6319201.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="KKO08_003036"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="KKO08_003036"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBD6319202.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="KKO08_003037"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="KKO08_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBD6319203.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="KKO08_003038"
     CDS             7138..7749
                     /locus_tag="KKO08_003038"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBD6319204.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KKO08_003039"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="KKO08_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBD6319205.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KKO08_003040"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="KKO08_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KKO08_003041"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="KKO08_003041"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBD6319206.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="KKO08_003042"
     CDS             complement(11943..12302)
                     /locus_tag="KKO08_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319207.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12805)
                     /locus_tag="KKO08_003043"
     CDS             complement(12299..12805)
                     /locus_tag="KKO08_003043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319208.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12951..13514)
                     /locus_tag="KKO08_003044"
     CDS             complement(12951..13514)
                     /locus_tag="KKO08_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319209.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="KKO08_003045"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="KKO08_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="KKO08_003046"
     CDS             complement(14722..15096)
                     /locus_tag="KKO08_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBD6319210.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..>15799)
                     /gene="wzy"
                     /locus_tag="KKO08_003047"
     CDS             complement(15100..>15799)
                     /gene="wzy"
                     /locus_tag="KKO08_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBD6319211.1"
                     /translation="IYEHLSLTSEFFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCL
                     IIVLAILLTESRGVLLFTTLSLLLASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSE
                     DSDSVRFNDLYFYYKNVDLATFLFGRGFGSFILDRLRIEIVPLEILQKTGVIGVFISL
                     VPMLLIFLKGYFLNSTKTSLMMSLILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSI
                     ENIQISNNLTSGAK"
BASE COUNT         4466 a         3770 c         3570 g         3993 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatt
//
LOCUS       DAECNO010000090        15408 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.090, whole genome shotgun sequence.
ACCESSION   DAECNO010000090 DAECNO010000000
VERSION     DAECNO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.090"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1173)
                     /locus_tag="KKO08_003048"
     CDS             complement(<1..1173)
                     /locus_tag="KKO08_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBD6319212.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="KKO08_003049"
     tRNA            complement(1372..1456)
                     /locus_tag="KKO08_003049"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="KKO08_003050"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="KKO08_003050"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBD6319213.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="KKO08_003051"
     CDS             2800..4302
                     /locus_tag="KKO08_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBD6319214.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="KKO08_003052"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="KKO08_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBD6319215.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="KKO08_003053"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="KKO08_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBD6319216.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="KKO08_003054"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="KKO08_003054"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBD6319217.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="KKO08_003055"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="KKO08_003055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBD6319218.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="KKO08_003056"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="KKO08_003056"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBD6319219.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="KKO08_003057"
     CDS             complement(11867..13063)
                     /locus_tag="KKO08_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBD6319220.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="KKO08_003058"
     CDS             13256..13759
                     /locus_tag="KKO08_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD6319221.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="KKO08_003059"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="KKO08_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBD6319222.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="KKO08_003060"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="KKO08_003060"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBD6319223.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3975 a         3932 c         3966 g         3535 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcgtcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcacc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt ctggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatggcg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatctg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaagt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccata caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgaatttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgag
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaatatc tctataaa
//
LOCUS       DAECNO010000091        15099 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.091, whole genome shotgun sequence.
ACCESSION   DAECNO010000091 DAECNO010000000
VERSION     DAECNO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.091"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..179
                     /gene="yjbD"
                     /locus_tag="KKO08_003061"
     CDS             <1..179
                     /gene="yjbD"
                     /locus_tag="KKO08_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBD6319224.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(312..1184)
                     /gene="rluF"
                     /locus_tag="KKO08_003062"
     CDS             complement(312..1184)
                     /gene="rluF"
                     /locus_tag="KKO08_003062"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBD6319225.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1532..2479
                     /locus_tag="KKO08_003063"
     CDS             1532..2479
                     /locus_tag="KKO08_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBD6319226.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2549..3355
                     /locus_tag="KKO08_003064"
     CDS             2549..3355
                     /locus_tag="KKO08_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6319227.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3365..3772
                     /locus_tag="KKO08_003065"
                     /pseudo
     CDS             3365..3772
                     /locus_tag="KKO08_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3772..4266
                     /locus_tag="KKO08_003066"
     CDS             3772..4266
                     /locus_tag="KKO08_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBD6319228.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4332..5129
                     /locus_tag="KKO08_003067"
     CDS             4332..5129
                     /locus_tag="KKO08_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBD6319229.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5140..5928
                     /locus_tag="KKO08_003068"
     CDS             5140..5928
                     /locus_tag="KKO08_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBD6319230.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5980..7209
                     /gene="sorE"
                     /locus_tag="KKO08_003069"
     CDS             5980..7209
                     /gene="sorE"
                     /locus_tag="KKO08_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBD6319231.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7591..8280
                     /gene="pepE"
                     /locus_tag="KKO08_003070"
     CDS             7591..8280
                     /gene="pepE"
                     /locus_tag="KKO08_003070"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBD6319232.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8371..10002)
                     /locus_tag="KKO08_003071"
     CDS             complement(8371..10002)
                     /locus_tag="KKO08_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBD6319233.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10222..13905)
                     /gene="metH"
                     /locus_tag="KKO08_003072"
     CDS             complement(10222..13905)
                     /gene="metH"
                     /locus_tag="KKO08_003072"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBD6319234.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14105..14929
                     /gene="iclR"
                     /locus_tag="KKO08_003073"
     CDS             14105..14929
                     /gene="iclR"
                     /locus_tag="KKO08_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBD6319235.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3591 a         3981 c         3938 g         3589 t
ORIGIN      
        1 gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat aaattaatgg
       61 tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag gcttataaac
      121 cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc aggcgctaat
      181 gaacaatcaa cgcctgagat tttcactcgt ctgtgtggac acaatcttcc cctcacccta
      241 accctctccc cgaaggggcg aggggactgt ccgagctctt tttggacgtt ttcatcagtc
      301 tgaggcgtta atcagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
      361 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
      421 cccgctgttt tcggtttggc cttcgcttta ggttttgcct cggaagagga attttcgatg
      481 agcttaaaga ggtcgattaa ctcatcgtcg gttaaatagc gccactcccc cagcggaatg
      541 ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
      601 tgctcgcaca tgcgccggat ctgacggttc agcccctgca ctagggtaat gcggaagaca
      661 aacggcgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcaccccc
      721 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
      781 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
      841 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
      901 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg
      961 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     1021 tttactttca caatgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     1081 aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     1141 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     1201 cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa cgtcgaaatt
     1261 aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga tgcaaatttg
     1321 cctggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg ctgatgacta
     1381 ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca aagagtgaaa
     1441 aatcgctttg aaaggccctc atgatggggt tatatcagat caagcggtga gaaagcgtat
     1501 ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata tccgtttgat
     1561 tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa tcgcgcgcga
     1621 actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg atcagggaat
     1681 ggtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc agcaactgaa
     1741 gcaaaagttt ggcctgaaag acgttgtggt ggtgtcggga aatgatgagg atgaagagac
     1801 tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc tggagcctgg
     1861 cgatattgtc ggtttttcct ggggtcgcgc ggtgagcgca ctggttgaaa acttgccgca
     1921 ggcggggcaa tcgcggcagt taatctgcgt accaattatt ggcggaccgt ccggtaaact
     1981 cgaaagccgc tatcatgtaa acacattaac ctacagcgcg gcagcgaagc tgaaagggga
     2041 atcgcatctc gcggattttc cggctctgct ggataaccca ttaattcgta atgggatcat
     2101 gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggata tcgccctggt
     2161 gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt atggtggtga
     2221 agagagtgac gacctgaatg cccgccaggt tgctggcgat atttgctcgc gcttttttga
     2281 tattcacggc gcaatggttg aaacgaatat gagcgaaaaa acgctctcta tcgaaatgaa
     2341 taaattaaag caggcgcgat attccattgg cattgccatg agtgaagaaa aatacagcgg
     2401 aattattggt gcactgcgtg gaaaatatat taattgtctg gtaacgaata gcagcacagc
     2461 tgaactatta ctgaaataaa aatcttcccg atgtttaccg taataaaaat tacggcgggt
     2521 ccctattatc taaaatcagg agtctgttat gcaaacgtgg ttaaatttgc aggataaaat
     2581 cattattgtc accggcggcg catccggtat tggtctggcg attgtagagg aattattagc
     2641 acaaggcgcg aatgtacaga tggtcgatat tcacggtggc gatggtcaat atgaaggtca
     2701 taaaggctat cagttttggc cgaccgatat ttctagcaca aaagaggtaa atcatacggt
     2761 agcggaaatt atccagcgtt ttggtcgcat cgacggtctg gtcaataacg ccggggtcaa
     2821 tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg cagtatgaac tcaacgaagc
     2881 tgcattcgaa aaaatggtca atatcaacca gaaaggtgtt tttctgatgt cgcaggcggt
     2941 ggcgcgacag atggtcaaac aacatgatgg cgtgattgtg aatgtttcct cagaaagtgg
     3001 gctggaaggc tcagaaggac aaagctgtta cgccgctacc aaagccgcgc tcaatagctt
     3061 cacgcgctcc tggtcgaaag agctgggtaa acacggtatc cgtgtggtcg gtatcgcgcc
     3121 cgggattctg gaaaaaaccg ggctacgcac gccggaatat gaagaggcgc tggcgtggac
     3181 gcgcaatatc accgtcgagc agctgcgtga aggctatacc aaaaacgcca ttcctattgg
     3241 gcgcgccgga agattagcag aagtagctga ttttgtttgt tatctgttgt ctgaacgcgc
     3301 cagctacatc accggagtaa ccactaacat tgcgggcggc aaaacgcgcg gctaaggagg
     3361 ctttatggtc aacgccattt tttgtgccca cggcaaatta gcttgcgcca tgctggaatc
     3421 ggttcagatg gtttacggca acgccaatgt tgaagccgtt gcatttgtgc ccggtgaaaa
     3481 cgccggagac attgcggtaa agctggagaa gttagtaagc attcacaatc aagatgagtg
     3541 gctgattgcc gtggatttac agtgcggcag cccgtagaat gcggcagcaa tgcttgcgat
     3601 gcgcaatcct cgtttgcggg taatcagcgg cctttctctg ccgctggcgc tcgaactggt
     3661 tgataaccag gacagcatga atgttgatga actgtgtgaa cacctgacgc aaatcgcaaa
     3721 gcaaacctgc gttgtctgga aacaactggc aacgacagag gaggatttct gatgaacatt
     3781 acgctcgccc gcattgatga ccgtctgatc cacggccagg tcactaccgt ctggtcgaaa
     3841 gtggctaacg cccagcgcat tattatctgc aatgacgaag tttataacga tgaagtgcgc
     3901 cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg ttaacgtggt caatattgaa
     3961 aaagccgtcg cggtttatca caacccgcaa tatcaggacg aaaccgtttt ttatcttttc
     4021 actcgcccac aggatgcctt agcgatggtt cgccaggggg taaaaatagg caccctgaat
     4081 attggcggca tggcctggcg gcccggtaaa aaacagttaa ccaaagcggt ttcattagat
     4141 gatgatgata ttaacgcatt tcatgagtta aataatcttg gtgtcatact ggatttacgc
     4201 gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa taaacgaaca gttaatcgca
     4261 aattaaataa tagcgcctgt gtacatatgc tttaacaggc atggatgacg acacttaaaa
     4321 ggtgacaaat catggaaata agtaccctac aaatcattgc tatatttctt ttttcctgta
     4381 ttgccggaat gggcagcgtg ctggatgaat ttcagactca tcgcccgtta attgcctgta
     4441 cggtgattgg tttaattctc ggtgatttaa aaaccggaat aatactcggc ggtacgctgg
     4501 aattgatagc tctctgctgg atgaacgtcg gcgcggcgca atctccggat tctgcactcg
     4561 ccagcataat ctccgccatt ctggttatcg ttggtcagca aagcatcgcc accggaatcg
     4621 ccatcgcgtt gcctgtggct gcggcaggcc aggtgctgac ggtgtttgcc cgtaccatca
     4681 ccgtagtgtt ccagcacgcg gcggataaag cagcagaaga ggcgcggttt cgcactctcg
     4741 atattctgca tgtctccgcg cttggcgtgc aggcgctgcg cgtcgccatt ccggcactga
     4801 ttgtctcact gttcgtcagc gccgatatgg tcagcaatat gttaaacgcc attcccgaat
     4861 ttgtgacccg aggactacag attgctggcg gttttatcgt ggtggtcggt tacgccatgg
     4921 tgctacgtat gatgggcgtg aaatatttga tgcctttctt tttcctcggt ttcctcgcag
     4981 gtggctacct cgatctcagc ctgctggcgt tcggtggcgt cggcgtgatc atggccctgg
     5041 tctacatcca gctaaatcca cagtggcgta aagctgaacc tcaactccag gccaccgcct
     5101 ccaccgccct tgaccaactt gacgattaac ggagccatca tggaacagag aaaaattaca
     5161 cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc aacaggcgtc ctttaacttt
     5221 gaacgtattc acgggctggg cttttgctac gacatgatcc ccgccatcaa gcgactttac
     5281 ccattaaaag aggatcaggt tgcggcgctc aggctacacc tagtgttctt caataccacg
     5341 ccagccgtat gtggcccggt catcggcgcg gaaattgatg acggtaccat caacggcatc
     5401 aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg gggaacgctg
     5461 cgcccgatta ccgccgcgct cggcgcatct ctggcacttt cgggcaacat tctcggcccg
     5521 ctgctgttct tctttatttt caacgcggtg cgtctggcga tgaagtggta tggcctacag
     5581 ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa
     5641 ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt gaccaaatgg
     5701 acgtcaatca acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc
     5761 accatgaccg tgcagaacat tctcgaccaa ctttgccctg gtttgctggc gctcggtctg
     5821 acgctactaa tggttcgtct gctcaacaaa aaaattaacc cggtatggct gattttcgcc
     5881 ctgtttggct tagggattat cggcaatgct ctgggcttcc tgtcctgatt cttcgccccg
     5941 gcacgactgc cgaggccatc gctcaacatg aggtggttta tgaaaacaac agctctgcgt
     6001 ctttatggta aacgtgattt acgcctggaa acctttgacc ttcctgaaat gcaggaggat
     6061 gaaatcctcg caacggtggt cactgacagc ctgtgcctct cttcctggaa agaggccaat
     6121 ctgggtgaaa accataaaaa agtacccgac gatgtgacga ccaacccaat catcatcggc
     6181 cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt ggcagcacaa attccagccg
     6241 ggtcagcgtt atgtgattca ggccaacctg caactccccg accgcccgga ttgccccggc
     6301 tactccttcc cgtgggtaga cggcgaagcg acgcatgtgg ttattcccaa cgaggtcatg
     6361 gaacaatatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc actggttgaa
     6421 ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga aggtagttat
     6481 aaccacacga tggggattcg cccgcaaggg cgcacgctga tcctcggcgg caccggacca
     6541 atgggactgt tggcgattga ttatgcgctg catggaccag ttaacccgtc gctgctcgtc
     6601 attaccgata ccgacaacga caaattgagt tatgcgcgca agcactatcc atcagaaccg
     6661 caaacactga ttcattatct caatgccacc gatgcagcat ttgatacgtt aatggcgctg
     6721 agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg actggtgact
     6781 ctcgcctctt ccttgctggc gacagatggt tgcctgaatt tcttcgccgg accgcaggat
     6841 aaacatttca gcgcgccaat taatttctac gatgtgcatt atgcatttac ccactacgtg
     6901 ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg tcaaactgat cgaagagaaa
     6961 aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc gggcgaaacc
     7021 acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg tgtataccgg gaaatacctg
     7081 ccgctgacgt cactcacgca gattcaggat caagcactgg cggcgattct ggcgcgtcat
     7141 caggggatct ggtccggtga ggcggagcaa tacctgctcg cccatgcaga ggcaatttcc
     7201 catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgcccca gcaacttcgc
     7261 gaccggatgt gcggctgatt gctgaagcag cacaatttgc acgagaagga taaaaaatat
     7321 cagcgcctta aggggcgctc aaattggtgg caaagtacac attgtttatg ccggatgcgg
     7381 aatgaagcct tattcggtct acataatttt gaaaatccaa tatattgcag ttatctggta
     7441 ggccaggtaa gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc agcgcgcggt
     7501 gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc
     7561 ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg
     7621 ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt
     7681 cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca
     7741 gagaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt
     7801 gtcgatcccg ttgccgcgat tgaaaatgcg gaaattgtga ttgtcggcgg cggtaatact
     7861 ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt
     7921 aaacgtggcg cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt
     7981 cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc
     8041 ccgttgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa aggtgaaacc
     8101 cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtctg
     8161 ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc
     8221 acttatgtgt ttaaggctgg tgaagaagcg gttccgctgg aagctggtca ccgtttttaa
     8281 gtgaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg
     8341 cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc
     8401 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc
     8461 tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgtacgtttt gctgatgcag
     8521 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
     8581 acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagctttaa
     8641 attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa gcgaaaatgc
     8701 ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga
     8761 ggcctgttca aggttgagcg acatttcgat gatctccgcc cagcggcgcg actcttcctc
     8821 cgccaactct tcttttggca tccgcgccag atacagctta atggcggtgt agagaacgtt
     8881 gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
     8941 attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc
     9001 gttcgccaga gcaagcgtgg gcgtgtccag cgcgctgaca tccagatgtt taggccgtag
     9061 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
     9121 aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac
     9181 cagttccgct tttggcaatg acagcttccc cattgtctct gccagcaaat ggacaaacgg
     9241 caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga cacggcgggc
     9301 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagctcc
     9361 aatcaccaga cagagcgcca cggggaagga gataatgcct gcagcggtca gagtcgcggt
     9421 cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc
     9481 atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt
     9541 gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcagcc caagaccaat
     9601 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
     9661 gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag
     9721 cgccgtcccg acatctgccc ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc
     9781 ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgta ccagtgcggt
     9841 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
     9901 acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac
     9961 cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag gttctcctta
    10021 tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat
    10081 ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt
    10141 ataagtgttt catattaata aagagacagg gcactcatta agcgccctgt ttgttgtaag
    10201 gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg
    10261 ctcaacatcg gagacgctca tacctttacg gcgggcatag tcttcaacct gatcgcgctg
    10321 aatttgcgcc accgcgtagt acttgctgtc cggatgactg aaataccaac ccgaaaccga
    10381 tgcaccaggc cacatcgcga aagattctgt gagtttcatg ccagtatgtt tttccacttc
    10441 cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccggat agcccggtgc
    10501 cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg
    10561 cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttcggc
    10621 taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa
    10681 ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc
    10741 tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt
    10801 tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc
    10861 acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg
    10921 cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag
    10981 ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact tcccggccag
    11041 cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagcgttt cgatgctggc
    11101 ttccacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa
    11161 tgcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgtg gtttcttgcg
    11221 cccgtgctga atacgcacgg tttcgtactc cttgcaggta cgagcgacaa aatcatcacg
    11281 ctgagtatcg gaaagcagcg ccgctaccac accaacggtg cgcgaggcat tctgcacata
    11341 caccgtaggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtggt
    11401 cgcgccgcca atcagtaacg gaatggcgaa gccctgacgc tccatctctt tcgccacgtt
    11461 aaccatctcg tccagcgacg gcgtgataag ccccgaaagg ccaatcaaat cagcattcac
    11521 ttctttggcg atacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc
    11581 gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt ggacatcgcc
    11641 tttcacggtg gcgatcacca tcttgccgtt ggttttaccc tgctctttgc tggcttcaat
    11701 aaacggttcg agatacgcca ctgcctgttt catgacgcgc gccgatttga ccacctgtgg
    11761 caggaacatt ttgccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg
    11821 gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcag tatcctgctc
    11881 gataaactcg gttatgcctt tgaccagcga gtattccaga cgtttattca cgtcccacga
    11941 gcgccactcc gcttgctggg cgttagcagt gtcgtccgct ttactgccgc gatatttctc
    12001 ggcaagctcc agtaaacgct cagtgccatc gtcacgacga ttaagaatca catcttccac
    12061 cgcgtcgcgc agttcagcgg gtaggtcgtc gtaaatcgcc agttgcccgg cgttgacgat
    12121 ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg
    12181 caccggatcg ttgccacgga atgagaaaga aacgttagat acgccgccgg aaatcagcgc
    12241 gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt
    12301 gtgctcttca ataccggtgg cgaccgcgaa gatgttcggg tcgaagatga tatcttctgg
    12361 cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg
    12421 tgcgcgagta tcggcctgtc cctgttcgtc aaaggccatc accaccactg ccgcaccgta
    12481 gcgacgcaac aatttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga
    12541 gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga
    12601 ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa
    12661 acgcaccatc gccgcttcgg catcgagcat cccttcatcc atgttgatat cgataatctg
    12721 cgcgccgttt tccacctgct gacgcgccac atccagcgcc tcgctgtatt tctcttcttt
    12781 gatcaggcgc ttgaacttag cagagccggt gacgttggtg cgttcaccca cgttcacaaa
    12841 caggctatcg tcgccaatgt tcagcggctc caggccagac aaacggcagg ctacgggaat
    12901 ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg
    12961 cggcgtggtc ccgcagcaac caccgacgat attgagaaag cccgcttgcg cccattcacg
    13021 tatctgtttt gccatcgtgt cggcgtcgag atcgtattca ccaaaggcgt taggcagccc
    13081 ggcgttcggg tgggcggtga cgtagcattc cgcaatccgt gatagttcct gcacgtactg
    13141 gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagcgctt cggcgtggcg
    13201 caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt
    13261 gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac
    13321 cgcaaagacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc
    13381 cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccgcca gctggtcaaa
    13441 agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt
    13501 tgggccaaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc
    13561 agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat
    13621 ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga tgatatccgc
    13681 gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttac tgagtaccag
    13741 caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca aagcgttcac cacgaaaatc
    13801 ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca acaccagaat
    13861 acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac
    13921 gctcaaccca gatttaataa aaattcaaca aaccatactg gaataaacgc atctgtggta
    13981 aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca
    14041 actattgcat tagctaacaa taaaaatgaa aataatttcc acgatacaga aaaaggaggc
    14101 tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc
    14161 agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga
    14221 atccaatggc agcgtggcac tcacagagct gtcgcaacaa gccgggttac ccaattccac
    14281 gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact
    14341 gggacactgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg
    14401 taatttgtta gcaattgttc accctatcct gcgcaatcta atggaagagt ctggcgaaac
    14461 ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca
    14521 gtgtacccac ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc
    14581 aggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca
    14641 ccgcaaaggg ttacatgcct atacccacgc aactctggtg tctcctgtgc atttaaaaga
    14701 agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcgctggg
    14761 gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc
    14821 tatttccgga ccgatttcac gtattaccga tgatcgcgtg accgagtttg gcgcgatggt
    14881 gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc
    14941 gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta
    15001 atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta ttaactcagc
    15061 attattttta aacatcaaac cacttaatta ttaacaaaa
//
LOCUS       DAECNO010000092        15052 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.092, whole genome shotgun sequence.
ACCESSION   DAECNO010000092 DAECNO010000000
VERSION     DAECNO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.092"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..177)
                     /locus_tag="KKO08_003074"
     CDS             complement(<1..177)
                     /locus_tag="KKO08_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319236.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNKSPKQTLLNQFSLCLKMEG
                     EEQLCDCVNELKHGL"
     gene            complement(593..1534)
                     /gene="fabY"
                     /locus_tag="KKO08_003075"
                     /gene_synonym="yiiD"
     CDS             complement(593..1534)
                     /gene="fabY"
                     /locus_tag="KKO08_003075"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBD6319237.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="KKO08_003076"
     CDS             complement(1579..2016)
                     /gene="dtd"
                     /locus_tag="KKO08_003076"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBD6319238.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2013..2885)
                     /locus_tag="KKO08_003077"
     CDS             complement(2013..2885)
                     /locus_tag="KKO08_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBD6319239.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="KKO08_003078"
     CDS             complement(2879..3478)
                     /gene="yihX"
                     /locus_tag="KKO08_003078"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBD6319240.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3577..4362)
                     /locus_tag="KKO08_003079"
     CDS             complement(3577..4362)
                     /locus_tag="KKO08_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBD6319241.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="KKO08_003080"
     CDS             complement(4396..5292)
                     /gene="yihV"
                     /locus_tag="KKO08_003080"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBD6319242.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5460..6356
                     /gene="yihU"
                     /locus_tag="KKO08_003081"
     CDS             5460..6356
                     /gene="yihU"
                     /locus_tag="KKO08_003081"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBD6319243.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6380..7258
                     /gene="yihT"
                     /locus_tag="KKO08_003082"
     CDS             6380..7258
                     /gene="yihT"
                     /locus_tag="KKO08_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBD6319244.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7275..8516
                     /gene="yihS"
                     /locus_tag="KKO08_003083"
     CDS             7275..8516
                     /gene="yihS"
                     /locus_tag="KKO08_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBD6319245.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8654..9556
                     /locus_tag="KKO08_003084"
                     /pseudo
     CDS             8654..9556
                     /locus_tag="KKO08_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9755..11791
                     /gene="yihQ"
                     /locus_tag="KKO08_003085"
     CDS             9755..11791
                     /gene="yihQ"
                     /locus_tag="KKO08_003085"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBD6319246.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11837..13211
                     /locus_tag="KKO08_003086"
                     /pseudo
     CDS             11837..13211
                     /locus_tag="KKO08_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13254..14657
                     /locus_tag="KKO08_003087"
     CDS             13254..14657
                     /locus_tag="KKO08_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6319247.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14725..>15052
                     /gene="ompL"
                     /locus_tag="KKO08_003088"
     CDS             14725..>15052
                     /gene="ompL"
                     /locus_tag="KKO08_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBD6319248.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3699 a         3875 c         3745 g         3733 t
ORIGIN      
        1 caaaccatgc ttcagttcgt taacacaatc gcacagttgc tcctcacctt ccattttaag
       61 gcaaagtgaa aattgattta atagcgtttg cttcggtgat ttattactca ttagttgttt
      121 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
      181 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
      241 agtaaatgaa tgatactgac aatgtatgcc agcccattat ttttgcataa tatttgtgag
      301 ctatagattt taaaaatatt tatgaaaaaa gtatggcacc agagggggtt ttgactatca
      361 aaaatcgctc aaacctgaac taatatgatg agttaagcat tatctgcaat aattgccact
      421 cagctctaca aataatattt aaatgctcct gaacagtgat gtagcaagac acgtatggtg
      481 ccaacgaaga ataaaaattt ataaagttct tattcaatat aaccggaata gtcagggact
      541 gatgtaaatg accggaatga atgagagagg cagcaccgcc cctctccata agctactctt
      601 cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc
      661 cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg
      721 cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg
      781 catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg
      841 ttccgccgag gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg
      901 ccagcgagaa taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg
      961 gcatggtggt gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac
     1021 taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca
     1081 aaatatcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg
     1141 ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt
     1201 cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg
     1261 tcatcgccat cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg accataaagc
     1321 gaatggacgc ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct
     1381 gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt
     1441 ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa cgctctaact
     1501 cttcttctgt ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg
     1561 acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag
     1621 cgatacctgc atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca
     1681 gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa
     1741 acttgggcgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac
     1801 actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg
     1861 gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac
     1921 acccaataac accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac
     1981 gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca
     2041 gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag
     2101 acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg
     2161 taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct
     2221 tcgaacagga gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg
     2281 gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata
     2341 cgcagcacgt tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag
     2401 gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac
     2461 acggcgaacg agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg
     2521 ctatcgatgg agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg
     2581 gtcatcttgt tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta
     2641 gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg
     2701 ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac
     2761 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     2821 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     2881 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     2941 taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3001 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     3061 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     3121 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     3181 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     3241 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     3301 cctctgcgaa cgcttcatcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     3361 tatgaaaact cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca
     3421 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3481 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3541 ggcaaggtat gaagccaggt taacggtcac aaaaatctac gcgtcttcct ggggaagaat
     3601 gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt
     3661 aaagagcgca gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt
     3721 agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt
     3781 agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt
     3841 taccagataa tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc
     3901 gctattatga gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta
     3961 aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa
     4021 cgcccgggca acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg
     4081 aatatagtct gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg
     4141 ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt
     4201 aatcaggcct tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc
     4261 cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag
     4321 ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac
     4381 catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg
     4441 gagcccggct cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg
     4501 gactgactcc gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac
     4561 atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc
     4621 accattttcc agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt
     4681 gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag
     4741 acccggttct gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg
     4801 cgtaatgtcc ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt
     4861 tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat
     4921 ttcctccaac cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg
     4981 ctcgcctttg gtatccacca tgatggcgga ttgcgaagat ttcgcctggt tataccgttt
     5041 ggtgtaacgg gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgcgtc
     5101 gtcatcacct acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc
     5161 tgccgtcgcc gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct
     5221 ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc
     5281 aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat
     5341 tgtttaaaaa gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca
     5401 gacaagcaaa gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta
     5461 tggcagcaat cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat
     5521 tgcagcaagg gcatcaactt cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg
     5581 tagacaaagg tgcgactccc gccgccaacc cggcgcaggc cgctaaagat gccgaattta
     5641 tcattaccat gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg
     5701 tttgcgaagg cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc
     5761 aaaccgataa attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg
     5821 taggccgtac ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg
     5881 ctgaacaagt tgaacgtgcc acaccgatcc tgatggcgat gggcagtgag ttgatcaacg
     5941 ctggcggtcc gggcatgggg atccgcgtta agctcatcaa caactacatg agcatcgcgc
     6001 tcaatgcgct ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg
     6061 ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg tcacttcacc acttcctggc
     6121 caaacaaagt cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg
     6181 atcttggcat cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg
     6241 cctcacggga ggtttatagc caggcgcgcg cagcgggtca cggtcgccag gactggtccg
     6301 ccattctgga acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac
     6361 tggataaagg aataaatgaa tgaataagta caccatcaac gacattacgc gcgcatcggg
     6421 cggttttgcc atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc
     6481 tggggcaccc gctccggtag ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa
     6541 ggccctctcg ccttatgcct cggcgattct ggtagatcaa caattctgct accgccaggt
     6601 ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat
     6661 tcctggcaac ggtattccgg tcgatagcgt ggttattgac cgcaaaatca atccgctaca
     6721 gatcaaacaa gacggtggca aagccttaaa actgctggtg ctgtggcgta gtgatgaaga
     6781 tgcgcagcaa cgtctggata tggtgaaaga gttcaacgaa ctgtgccact cacacggtct
     6841 ggtaagcatc attgagccag tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga
     6901 acaagcgatc atcgatgccg ccaaagagct gggcgacagt ggcgctgacc tctacaaagt
     6961 tgaaatgccg ctttatggca aaggtccgca acaagagctt ctctgtgctt cacaacgtct
     7021 gaatgaccat atcaatatgc catgggtgat cctctcttcc agtgtcgacg aaaaactgtt
     7081 cccgcgtgcc gtacgcgtgg caatgacggc gggcgcatcg ggattcctgg caggccgtgc
     7141 agtctgggca tcggtcgtcg gtttaccaga caacgagctg atgctccgtg acgtttgcgc
     7201 accgaaatta caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgctaaga
     7261 tgaggataaa aggaatgaaa tggtttaaca ccctaagcca caaccgttgg ctggaacagg
     7321 aaaccgaccg catctttgat tttggtaaaa attccgtagt gccgactggt tttggctggt
     7381 taggcaataa agggcaaatc aaagaagaga tgggcaccca tctgtggatc acagctcgta
     7441 tgttgcacgt ttattccgtt gctgcggcga tgggtcgacc tggcgcttac gcgttggttg
     7501 atcacggtat taaagccatg aacggcgcac tgcgtgataa aaaatatggc ggctggtatg
     7561 cctgcgtgaa tgacgagggc gtggtggatg cctccaaaca gggctatcaa cacttctttg
     7621 ctctgctggg tgccgccagc gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg
     7681 attacaccat tgagattatc gagaaatatt tctggagcga agaagagcag atgtgcctgg
     7741 agtcctggga cgaagccttc agcaaaaccg aagagtaccg cggcggcaat gccaatatgc
     7801 acgcggtgga agctttcttg attgtttatg acgtcactca cgacaaaaaa tggctggatc
     7861 gcgcgattcg cgtggcttcc gtgattatcc acgacgtcgc cagaaataat cattatcgcg
     7921 ttaacgaaca cttcgatacc cagtggaatc cgctgccgga ttacaacaaa gataacccgg
     7981 cgcatcgctt ccgcgcgttc ggaggtacac caggccactg gatcgaatgg ggccgtttaa
     8041 tgctgcacat ccatgctgcc ctggaagccc gttgcgaaca accaccagca tggctgctag
     8101 aagatgccaa aggtctgttt aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg
     8161 acgggattgt ttataccgtt gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt
     8221 ggcctatcgt cgaagcaatg ggtacggcct acgcgctcta caccgtcact ggcgatcgcc
     8281 agtatgaaac ctggtatcaa acatggtggg agtactgcat taagtacctg atggactatg
     8341 aaaatggttc ctggtggcag gagctggatg cggacaataa ggtcatcacc aaagtctggg
     8401 acggcaaaca ggatatttat cacctgctgc attgcctggt gatcccgcgt atcccgttag
     8461 cgcccggcat ggctccagcg gttgcagcgg gtctgctgga tattaatgcg aaataactga
     8521 tctgaaaata gggtgcgggt tttcccctca ccctaaccct ctccccaaag gggcgagggg
     8581 accgtccggt gcagtttttg ggtttgacat cagcctcacg ggaggctgta tgtcgttaat
     8641 taaggtgcct tgtatgcaaa tcaccaatat gcattgcagt ggacagaccg tttcacttgc
     8701 cgcgggggat tatcacgcga ccattgtgac cgtcggtgct ggtctggctg aattaacctt
     8761 tcagggttgt catttagtca tcccgcacaa acccgaagag atgccgctgg cgcatctggg
     8821 taaagtgctt attccgtggc cgaaccggat cgccaacggc tgttaccgtt atcaggggca
     8881 ggaatatcag ttacccatta atgaacatag ttcaaaagcc gctattcacg ggctgcttgc
     8941 ctggcgagac tggcaaatta gtgaattaac caccaccagc gttaccctga cagcttttct
     9001 gccgcccagt tacggctatc cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc
     9061 acacacgggt ctttccgttg agatcgcaag ccagaatatc ggcactgtcg cagcgcctta
     9121 tggcgtgggt attcatcctt atttaacctg caacttaaca tctgttgatg aatatctgtt
     9181 ccagcttccg gcaaactagg tttatgccgt cgatgaacac gtaaatccga caacgcttca
     9241 ccacgttgat gagctggatt taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat
     9301 cgaccacacc tttaaaaccg ctaatgattt gtgggaaatg accattaccc atccacagca
     9361 ggcgctttcg gtaagccttt gttccgatca accctgggtg caggtttata gcggtgaaaa
     9421 attacagcgt cagggactgg cagttgaacc tatgagttgc ccgcctaacg cctttaattc
     9481 aggtatcgac ttattattac tggaaccagg aaaaccgcac cgcctgtttt ttaatattta
     9541 cggacagaga aaataacttg tattgattag cagtcaaaat cattgaagaa catttcactc
     9601 gctaaaggca acattatgta tcttcaattt attaagttaa aaacagctca gcttctgtaa
     9661 agtttttata tatcagtttt atgagaatca ttttacataa gcctgtaata acaggaaggc
     9721 aacaatctcg ttctcaactc tatggagatt agttatggat acgccacgtc cacagttatt
     9781 agattttcaa tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat
     9841 tttaacccat aacaaagata atccttgttt atggattggc tcaggtatag cggatatcga
     9901 tatgttccgc ggtaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga
     9961 cgccatcgtc agccagtcac cggatggttg gttaattcat ttcagccgtg gttctgacat
    10021 tagcgccacg ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa
    10081 cgacaacctt aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat
    10141 ctacggctgc ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg
    10201 gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg
    10261 caaagaaaat gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag
    10321 cacgcagaag tattactgcc atgttgataa cagttgctac atgaacttcg actttagcgc
    10381 cccggaatac catgaactgg cgctgtggga agacaaagct acgctacgtt ttgaatgtgc
    10441 tgacacatac atctccctgc tggaaaaatt aactgccctg ctgggacgcc agccagaact
    10501 gcccgactgg atttatgacg gggtaacgct cggcattcag ggcgggacgg aagtgtgcca
    10561 gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga
    10621 ctggtctggg atccgtataa cctcttttgg caagcgcgtg atgtggaact ggaagtggaa
    10681 cagcgaaaac tacccgcaac tggattcacg cattaagcag tggtatcagg agggcgtgca
    10741 gttcctggcc tatatcaacc cgtatgttgc cagcgataaa gatctctgcg aagaagcggc
    10801 acaacacggc tatctagcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga
    10861 gttttacggc ggcgttgtcg atctcactaa tccagaagcc tacgcctggt tcaaggaagt
    10921 gatcaaaaag aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta
    10981 tctgcccacc gacacgtacc tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg
    11041 gcctgcgctg tgggcgaagt gtaactacga agcccttgaa gaaacgggca agctcggcga
    11101 gatcctcttc tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg
    11161 ggcgggcgac cagaacgtgg actggagtct cgacgatggc ctggcgtccg tagtcccggc
    11221 ggcgctgtcg ctggcaatga ccggacatgg tctgcatcac agcgacattg gcggttacac
    11281 caccctgttt gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc
    11341 cttcacgccg atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga
    11401 cggcgatgcg gaaactatcg cccattttgc ccgtatgact accgttttca ccaccctgaa
    11461 accttatctg aaagaggccg tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc
    11521 gctgttcctg cattacgaag acgatgcaca tacctattcc ctgaaatatc agtacctgtt
    11581 aggccgcgac attctggtcg ctccggtaca cgaagaaggt cgcagcgact ggacgctcta
    11641 tctgccggag gataactggg tacatgcctg gacgggtgaa gcgttccggg gcggggaagt
    11701 taccgttaat gcgcccatcg gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc
    11761 ggcactgttc gcgtcgttaa acagcatcta atcacatccg cccgtgggga aactcatggg
    11821 caaagggaga acatcgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc
    11881 ctttaaagag aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat
    11941 cggtacgctt tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg
    12001 cggcattatc tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat
    12061 tatgttggat tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta
    12121 tgcgtcattc ccggtcacct tactggcgat cgccaacttt gtcggcacac cgtttgatgt
    12181 caccggtaaa acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat
    12241 gatgaactgc tcctacggcg caatggtccc ggcaatcacc aaaaacccca acgaacgcgc
    12301 ctcactggcg gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg
    12361 attcgtgcca gttatgaatc ttatcgaagg taatcagaaa cttggctata tcttcgccgc
    12421 cacgctgttt tcactgttcg gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga
    12481 gcgttatgtc gaaacccaac caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt
    12541 ccgcgcaatt gccggtaacc gcccactgtt cattctgtgt attgccaacc tctgcacctt
    12601 aggggcgttt aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga
    12661 tcccatcctg ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt
    12721 cctgatgcct ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct
    12781 gatttgggtg ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt
    12841 ggcgttctcc tgcctggcgt tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca
    12901 ccgtcgagta cggcgagtgg cgcaccggcg tgcgttcgga aggcacggtc tacaccggct
    12961 tcaccttctt tcgcaaagtg tctcaggcgc tggctggttt cttccccggc tggatgctga
    13021 cgcaaattgg ctatgtgccg aacgtcgcac aggctgacca caccattgaa gggttacgcc
    13081 agttgatctt catctaccca agcgcactgg cggtagtcac cattgtggcg atgggttgct
    13141 tctacagcct gaacgagaag atgtatgtcc gcattgtgga agagatagaa gcccgtaaac
    13201 gcacggcgta ataataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg
    13261 accataatcc actgacatta aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg
    13321 acgtcggttc gaatttaatg ctctgcatcg gtactctgta tctcctcaaa ctttataccg
    13381 atgaattagg gatgcctgct tactatggcg ggattatctt tctggtcgcg aagtttttta
    13441 ccgcgtttac cgatatgctc accggatttt tactcgactc gcggaaaaat attgggccta
    13501 aaggcaaatt tcgtcccttt attttatatg cagcagttcc ggcggcgtta attgcaacgc
    13561 tccagtttat cgccaccacc ttttgtttgc cggttaaaac gacgattgcc accacgctgt
    13621 ttatgatgtt tggactttca tacagtctga tgaactgctc gtatggtgcg atgatcccgg
    13681 caattaccaa aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa
    13741 ccatagggct attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact
    13801 caaccgtcgg ttatgcctgt gcggcactta tgttctccat tggcggcttt atttttatga
    13861 tgctgtgcta cagaggcgtc aaagagcatt atgtggacac agcgccaacc ggacataaag
    13921 ccagtattct caaatctttt tgcgcgatat ttcgtaatcc accattgctg gttttatgca
    13981 ttgctaacct gtgtactctg gcggcattta atatcaaact ggcgattcag gtctattaca
    14041 cccagtatgt gctgaatgat attaatttat tgtcgtggat gggattcttc agtatgggat
    14101 gcatcctcgt cggcgtatta ctggtgccag taactgtaaa atgttttggt aaaaaacagg
    14161 tttatttagc tggcatggtg ctatgggcgg tgggtgatat actgaattat ttctggggaa
    14221 gtaactcttt cactttcgtc atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa
    14281 acagcctgaa ctgggcactg gtaccagata ccgtagatta cggtgaatgg aaaaccggta
    14341 ttcgtgccga aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac
    14401 ttgctggctt cttgccaggc attatgctga cgcaaattgg ttatgttccc aacatcgcgc
    14461 aaagcgatgc gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg
    14521 caattattgc tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcat
    14581 tgattattga ggaaatcaac caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg
    14641 cttccgtaac gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc
    14701 ctacgtttaa aaaatggact tattatgaaa aagattaatg caataatttt attatcttct
    14761 ctgacctctg cctcggtatt tgctggcgca tacgtcgaaa acagggaggc atataatctt
    14821 gcctccgatc agggcgaggt tatgctacgt gtgggttata acttcgatat gggcgcgggt
    14881 attatgttaa ccaataccta cacctttcag cgagaagatg aactaaaaca tggctataat
    14941 gaaattgaag gttggtatcc tttattcaaa ccaaccgata aattaaccat ccagcccggt
    15001 ggcttaatta atgataagag tatcagttcg ggtggtgcag tgtatctgga cg
//
LOCUS       DAECNO010000093        15031 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.093, whole genome shotgun sequence.
ACCESSION   DAECNO010000093 DAECNO010000000
VERSION     DAECNO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.093"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..237
                     /locus_tag="KKO08_003089"
     CDS             <1..237
                     /locus_tag="KKO08_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBD6319249.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="KKO08_003090"
     CDS             247..885
                     /locus_tag="KKO08_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBD6319250.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="KKO08_003091"
     CDS             888..2051
                     /locus_tag="KKO08_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBD6319251.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..3760
                     /locus_tag="KKO08_003092"
     CDS             2135..3760
                     /locus_tag="KKO08_003092"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBD6319252.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="KKO08_003093"
     CDS             complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="KKO08_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBD6319253.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="KKO08_003094"
     CDS             complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="KKO08_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBD6319254.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4812..5561
                     /gene="yjfP"
                     /locus_tag="KKO08_003095"
     CDS             4812..5561
                     /gene="yjfP"
                     /locus_tag="KKO08_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBD6319255.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="KKO08_003096"
     CDS             complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="KKO08_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBD6319256.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="KKO08_003097"
     CDS             complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="KKO08_003097"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBD6319257.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7840..9237
                     /gene="ulaA"
                     /locus_tag="KKO08_003098"
     CDS             7840..9237
                     /gene="ulaA"
                     /locus_tag="KKO08_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBD6319258.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9253..9558
                     /gene="ulaB"
                     /locus_tag="KKO08_003099"
     CDS             9253..9558
                     /gene="ulaB"
                     /locus_tag="KKO08_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBD6319259.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9568..10032
                     /gene="ulaC"
                     /locus_tag="KKO08_003100"
     CDS             9568..10032
                     /gene="ulaC"
                     /locus_tag="KKO08_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBD6319260.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10046..10696
                     /gene="ulaD"
                     /locus_tag="KKO08_003101"
     CDS             10046..10696
                     /gene="ulaD"
                     /locus_tag="KKO08_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBD6319261.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10706..11542
                     /gene="ulaE"
                     /locus_tag="KKO08_003102"
     CDS             10706..11542
                     /gene="ulaE"
                     /locus_tag="KKO08_003102"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBD6319262.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11542..12228
                     /gene="ulaF"
                     /locus_tag="KKO08_003103"
     CDS             11542..12228
                     /gene="ulaF"
                     /locus_tag="KKO08_003103"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBD6319263.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12357..12632)
                     /locus_tag="KKO08_003104"
     CDS             complement(12357..12632)
                     /locus_tag="KKO08_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6319264.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12959..13354
                     /gene="rpsF"
                     /locus_tag="KKO08_003105"
     CDS             12959..13354
                     /gene="rpsF"
                     /locus_tag="KKO08_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBD6319265.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13361..13675
                     /gene="priB"
                     /locus_tag="KKO08_003106"
     CDS             13361..13675
                     /gene="priB"
                     /locus_tag="KKO08_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBD6319266.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13680..13907
                     /gene="rpsR"
                     /locus_tag="KKO08_003107"
     CDS             13680..13907
                     /gene="rpsR"
                     /locus_tag="KKO08_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBD6319267.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13949..14398
                     /gene="rplI"
                     /locus_tag="KKO08_003108"
     CDS             13949..14398
                     /gene="rplI"
                     /locus_tag="KKO08_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBD6319268.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14469..>15031)
                     /locus_tag="KKO08_003109"
     CDS             complement(14469..>15031)
                     /locus_tag="KKO08_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBD6319269.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     HTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3512 a         3666 c         4050 g         3803 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccaataa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gttccggcaa
     3421 tattatgtgc ctggatgtgt tgcgcgttct caataagcaa gcgggcgtat acgacttatt
     3481 gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg cggttcgtcg
     3541 tttacagcag cagctgcgta agccagctga agaactgggg cgagagatta ctcatcagct
     3601 attcctgctg ggctgcggtg cgcaaatgtt gaaatatgct tctccgccaa tggcgcaggc
     3661 gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc agatccagaa
     3721 tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa gcgtatacga ctgatgcgac
     3781 gctggtttcg attaactaaa tgaaatatgt gaaaattgta ggccggacaa ggcgctcgcg
     3841 ccgcatccgg cattgttcat ttgctgtaga tgacactcat gcatacagta tcgcctgtac
     3901 gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc atgacgcacc
     3961 ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc ctgaaatatt
     4021 gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac tggcgaattc
     4081 tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg gtgaggcaag
     4141 taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg cattccatcg
     4201 cggtaataaa cgcccaagct tccagccggt aatttattgt gcacaggtaa acgcataggg
     4261 acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg tacaaaatgg
     4321 cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca acgtaataat
     4381 cagcttttgc agcgacagct tttgctctga tttctgctaa tgcgtcatcc ggagaacccc
     4441 gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg cggcgaatct
     4501 cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt gcgctacagg
     4561 cacttaacat caacaccaat aataaactgg caaaccggta aataacgcta ttacgtttcc
     4621 tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc cgaattgtta
     4681 cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc tcaacattat
     4741 gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata ccagacaaaa
     4801 aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc gttcttcatg
     4861 cttatcctgt cgggcaaaaa gataccccgt taccgtgcgt aattttttat cacggcttta
     4921 cttcatccag cctggtgtat agctattttg ccgttgcgct ggcgcaggct ggtttgcggg
     4981 tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgaa gcgcggcggt
     5041 taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactact ttacgtgcgg
     5101 caatagccga agaaaactgg ctgcttgatg accgtctggc agtcggtggc gcgtcgatgg
     5161 gcgcgatgac ggcactggga attaccgctc gccaccccac ggtgaaatgt accgccagca
     5221 tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg ctgatacctg
     5281 aaacggcagc acagcagaat gaattcaata aaattgtcgc gccgctggca gagtgggaag
     5341 cgacaaacca cctggaacaa cttggggaca gacctctact gctgtggcat ggcctcgacg
     5401 atgacgttgt gcctgccgac gaatcactac gtttgcagca ggccttaagc gagacgggac
     5461 gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgtatt acgcctgagg
     5521 cgttagatgc tgccgtgaca tttttccgcc agcatcttta aacacgcaga atgctgaccc
     5581 cttgcgcttc cagttgttgc aggatttccg ggttagcatt tttgccggtg atcagcatat
     5641 cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta ctgctatcaa
     5701 ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt gctgtcagca
     5761 tatcggtttt atacaaccct tctgcggtca gcccttttcc gctggtaaac atccagtgcc
     5821 cggcatagag actgttttcg ctgccctgcg ggcttaaagt gatggactga cttttgttgt
     5881 actgtccacc cataatgatc acgctgtcat gttcttgatc gatcaggtaa tttgccagcg
     5941 gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca agcagaaacg
     6001 cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc gaggccgctt
     6061 tagcaatacg tactttttca tcgtgattct gcgcctgatg cagattcatc ggcgtccagc
     6121 gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ctttttcagt ttgccgcttt
     6181 cgccaagttt attgatatcg cgtcgcgcag tggcaggcga aattcccaga cgctcaacga
     6241 ctttctcaac ggtcacaaag cccaattgtg cgagcatttc caggaggatt tgatgtcttt
     6301 gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata tttgaaatag
     6361 ccaggaaata ctacgccgga tagcgcgaac ttctccacgc tatccggtat aaacgacaga
     6421 ttacaggaat gacttgaacg gcagatccgg ttcaatagtg aagcaatcat cgaaaccgcg
     6481 cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc cgccaacctg
     6541 ccagataaac ggcttgaagc catacttcag acgatctttt ttgatctccc acagcacgcg
     6601 gatctcttgc ggatcggcct ggaagtttga ccagatgtcg tggtggaacg ggataaccac
     6661 tttcgcattc agggcttcac ccatacgcag catatcggcg ctggtcattt tgtcggtaat
     6721 accgcgcggg ttctcaccat acgatcctaa cgccacatcg atctgatgtt cgttaccgtg
     6781 cttcgcataa tagttagagt agtgggaatc gccactgtga tatagggaac cgccaggggt
     6841 tttgaacagg tagtttactg cgcgatcgtc catgccatct ggcagtacgc cagccgcttt
     6901 ttgatcggca ggcagggtga tcagtgcagt acggtcgaaa gcgtcaagcg catgaatttc
     6961 aatgtctttc acttttacaa catcgcccgg tttgaccacg atgcaacgct ctttcggtac
     7021 gccccagcca atccacaaat caacgcaggt tttcggtcca atgaatggta cgtcatcagc
     7081 acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt gatcgtgagt
     7141 cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg gggtggtgcg
     7201 taggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac cctgtttcat
     7261 taacgggtta ccgtgacttt gtttgccagt gccgcaccag aaatcaacgc aaacgttggt
     7321 gccaccttct gatttcaacc agatcccggt gcagcctagc caccacatcg caaatgtgcc
     7381 aggggcgacc tgctcttgtt caatttcttc attcagccag ctaccccact ccgggaaagt
     7441 gctcaggatc caggattctc gggtgatact tttcacttta ctcatcgcca tttaccttca
     7501 tgatagttca attcgaatca acatgtgatt ggttttgatt aatcctgaca ctattccttc
     7561 aggaaggcaa tgaccatttt ttgacttttt gccagggaag ttgttgttga ttttgagtgt
     7621 ggaaagattt aatggaatgt gtagttaaat taactaaatg aatttaaata aataattgtt
     7681 tcgttgtgtg aatcacactc tatccgtgtg gaattatttg cgggtcgcgc cacatttaat
     7741 caaaaataat cttgttgtga ttacttttga aaattagagt gagtacacaa cattccgggt
     7801 gtgcggaata cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc
     7861 tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc
     7921 tgtctgggct acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa
     7981 accataattg gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa
     8041 ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc cgatacttac
     8101 gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct atagctgggt aggttacgcg
     8161 gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt
     8221 cgtaccatca tgctgactgg tcatatcatg ttccagcagg cggggctgat tgccgttacg
     8281 ttgtttattt tcggctactc catgtggaca accattatct gcacggcgat tctggtttcg
     8341 ctctactggg gcattacctc caacatgatg tacaagccga ctcaggaagt gacggacggc
     8401 tgcggtttct ccatcggtca ccagcaacag tttgcatcat taattgccta taaagttgcg
     8461 ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg ctggctgaat
     8521 attttccacg acaacatcgt ctccacggcg attgtcatga ccatcttctt tggtgccatt
     8581 ctgctctcct tcggtatcga caccgtgcag gcgatggcag gcaaagtgca ctggacggtc
     8641 tatatcctgc aaaccggttt ttccttcgcg gtggcgatct tcatcatcac tcagggtgtg
     8701 cgcatgtttg tggcggaact ctctgaagcg tttaacggta tctcccagcg cctgatccct
     8761 ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgctccgaa cgccgtggtc
     8821 tggggcttta tgtggggcac catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc
     8881 ggttcctcga tcctgattat cccaggcttt atcccgatgt tcttctccaa cgccaccatc
     8941 ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg
     9001 gggatgatcg aaatctttgg ttgcgtctgg gtggtgaaac ttaccggtat gagtgcctgg
     9061 atggggatgg cggactggtc gattctggca ccgccgatga tgcagggctt cttctccatc
     9121 ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc
     9181 cgcgcactgc gcgcagaaga agatgcagaa aaacaattgg cagaacagtc tgcttaataa
     9241 ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc
     9301 atgatcatga agatgaaagt ggaccagttt ttaacccaat caaacattga ccacacggta
     9361 aatagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat catcatcgct
     9421 tctacgcaca ttgcgggcga aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc
     9481 aacatgctct ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agcgcatttc
     9541 ccgcaggatg tgaagtaagg acgcgccatg aaattacatg attcgctggc ggagaataaa
     9601 tccatccgcc tgcaggctga agctgagaca tggcaggacg cagtaaaaat cggcgttgac
     9661 ctgctggtgg cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt
     9721 gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg
     9781 gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc
     9841 aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac
     9901 acgcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc
     9961 gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac
    10021 gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa
    10081 ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat
    10141 cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc
    10201 gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct
    10261 ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat
    10321 caacaccgcc aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga
    10381 actgaccggt tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca
    10441 ggtggtttat caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga
    10501 tatcactgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct
    10561 ggcgctggaa gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg
    10621 cagcatccgt gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc
    10681 cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga
    10741 aaaagcgctc cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg
    10801 cttcgatttt gtcgagatgt cggtagatga aactaacgag cgcctgtcgc gcctcgactg
    10861 gagccgcgaa cagcgtctgg cgctggtgaa tgctattgtt gaaaccggcg tgcgtgtgcc
    10921 gtccatgtgc ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg
    10981 ggcgcagggg ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg
    11041 cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg
    11101 tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct
    11161 ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc
    11221 gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg
    11281 ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt
    11341 gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt
    11401 cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat
    11461 gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgcgca tggccaaagc
    11521 aggcatggtg gaggcggcat aatgcaaaag ctaaaacagc aggtgtttga agccaacatg
    11581 gatctgccgc gctatgtgct ggtgaccttt acttggggca acgtcagcgc tatcgaccgc
    11641 gaacgcgggc tggtggtgat caagcccagc ggcgttgcct acgaaaccat gaaagcagac
    11701 gatatggtgg tggtcgatat gagcggcaag gtggtggaag gggagtatcg cccatcttcc
    11761 gacactgcga cgcatctcga actctaccgt cgttacccgt cgcttggcgg cattgtccat
    11821 acccactcca ctcatgccac cgcatgggcg caggcggggc tggcgatccc ggcattaggc
    11881 accacgcacg ctgactactt ctttggtgat atcccgtgta cgcgtggatt aagcgaagaa
    11941 gaggtgcagg gtgagtatga actgaacact ggcaaagtga ttatcgaaac gctgggcaac
    12001 gccgagccgc tgcatacgcc ggaaattgtg gtgtatcagc acggaccgtt cgcctggggg
    12061 aaagatgccc acgacgcggt gcataacgcg gtagtgatgg aagaagtggc gaaaatggcg
    12121 tggattgcgc gcagtattaa tccacaactc aatcatatcg acagctacct gatgaataaa
    12181 cacttcatgc gtaagcacgg tcctaacgct tattacgggc agaagtaaaa cacgcgctgc
    12241 ggaaatttcc ttcctcggga gataactggt ctaattccgc agccgttttt caaaaaaagc
    12301 cccctgcgaa gggggcaaag caaactatgg caatgtttcg ttggttatac ctggtgctag
    12361 cgataaatat ccgcgctggc gtgcatattg ccgttactcc ccggttcccg catcagaatt
    12421 acgtggtagt acgttgcgcc ttgcgcatct gtttcttcat ttaatgcctg acgtgcttcg
    12481 ctttcagtgg cgaaattatg attgatataa atcacgccta agctttgcac atcgtccata
    12541 tttctggcct ggtggttatt aatttcaatg gctgcccatg tatttgcact tagcaaaagc
    12601 acagccagaa gggctaaaac tcgactgaac atagatacct cctcgacggc tgactttgtg
    12661 tgctctcctc tgtgatgatc ttctgattta attttaatca atgataaaga agttgatggt
    12721 gaccatttct gatgcagttg ttcaaaaaaa cactgtgacg gaatgtgatg aacttcaaat
    12781 cagcggttta gaggttattt gcgaaagggg agatttattt ctgctccgcc cttgagttta
    12841 gcggggtatg ccagtactat aacgcgtcat ttttcagccg acctttaaca cgttccttgc
    12901 ctccccggga ttcggctgac ccagacagga ggctgaataa tccgtaagga gcaattcgat
    12961 gcgtcattac gaaatcgttt ttatggtcca tcctgaccag agcgaacagg ttccgggcat
    13021 gatcgagcgc tacactgctg ccatcactgg tgcagaaggc aagatccacc gtctggaaga
    13081 ctggggccgc cgtcagctgg cttacccgat caacaaactg cacaaagcac actacgttct
    13141 gatgaacgtt gaagctccgc aggaagtgat cgatgagctg gaaactacct tccgcttcaa
    13201 cgatgccgtt atccgcagca tggttatgcg taccaagcac gctgttaccg aagcatctcc
    13261 gatggttaaa gcgaaagacg agcgccgtga gcgtcgcgat gatttcgcaa acgaaaccgc
    13321 tgatgatgct gaagctgggg attctgaaga gtaatttctg atgaccaacc gtctggtgtt
    13381 gtccggcacc gtgtgcagga ctccccttcg aaaggtcagt ccatcaggaa ttcctcactg
    13441 ccagttcgtg cttgagcatc gttctgtgca ggaggaagcc ggctttcacc ggcaggcatg
    13501 gtgtcaaatg cccgttattg ttagcggaca cgaaaaccag gccattactc acagtataac
    13561 ggtcggcagt cgcataaccg ttcaggggtt catttcatgc cacaaggcaa agaacggact
    13621 gagcaaaatg gttttgcatg ccgagcagat tgaattgata gattctggag actagccata
    13681 tggcacgtta tttccgtcgt cgcaagttct gccgtttcac cgcggaaggc gttcaagaga
    13741 tcgactataa agatatcgct acgctgaaaa actacatcac cgaaagcggt aagattgtcc
    13801 caagccgtat caccggtacc cgtgcaaaat accagcgtca gctggctcgc gctatcaaac
    13861 gcgctcgcta cctgtccctg ctgccgtaca ctgatcgcca tcagtaatcg gtcacggtcc
    13921 attaatacga ctttgagagg ataaggtaat gcaagttatt ctgcttgata aagtagcaaa
    13981 cctgggtagc ctgggtgatc aggtaaacgt taaagcgggc tatgctcgta acttcctggt
    14041 accgcagggt aaagctgttc cagctaccaa gaaaaacatt gaattcttcg aagcacgtcg
    14101 cgctgaactg gaagctaaac tggctgaagt tctggcagct gctaatgctc gcgctgagaa
    14161 aatcaatgca ctggaaactg ttaccatcgc gtctaaagct ggcgacgaag gtaaactgtt
    14221 cggttccatc ggtactcgcg acatcgctga cgctgtaact gcagctggcg ttgaagtggc
    14281 taagagcgaa gttcgtctgc cgaacggcgt tctgcgtacc actggcgaac acgaagtgag
    14341 cttccaggtt cacagcgaag tattcgcgaa agtgatcgta aacgtagtag ctgaataatt
    14401 cgttattcaa cgagacgtaa aaacgccgac cattggtcgg cgttttgctt tctatttttc
    14461 gtcaggcatt aatttcgcaa gtagatccat tcaccattct cttgtgtcca taagtcagga
    14521 tggtttgctg gcgttagatg catacgatca ctccttatgt ctcggtcctg cggcactcgc
    14581 tcgaaaccta aacccgagta gattgcggct gaatttgata aggaacgtag accaattttc
    14641 aaatcatggg gtaaatcaga atacgcgttt agcaatgcag caatgatgaa tccgagggca
    14701 ttagaaacgc ctgtaacagt gtgaagttgg tcgatataaa gatcgattgt tggtagttca
    14761 gatgaccagc aattaggcat ttgggcagga agggttacaa cgtatcctat cgtttggccg
    14821 gtatagtctg gagtactata accaggtccc aaaacaacct cgtgtattgt gtatttactg
    14881 ttgggttcga aggattcgac atcttgcaat aaatatttca gttctgcggt taacctcgca
    14941 cccgcgtaag ctttattgta tttctgggcg tgaatattat ctaatgtagc ttcatggttc
    15001 catccaaaag aattatcacc attcattgtt a
//
LOCUS       DAECNO010000094        14945 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.094, whole genome shotgun sequence.
ACCESSION   DAECNO010000094 DAECNO010000000
VERSION     DAECNO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.094"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..938)
                     /locus_tag="KKO08_003110"
     CDS             complement(<1..938)
                     /locus_tag="KKO08_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBD6319270.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALA"
     gene            complement(1084..2043)
                     /locus_tag="KKO08_003111"
     CDS             complement(1084..2043)
                     /locus_tag="KKO08_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HBD6319271.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            complement(2146..2556)
                     /gene="uraH"
                     /locus_tag="KKO08_003112"
     CDS             complement(2146..2556)
                     /gene="uraH"
                     /locus_tag="KKO08_003112"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBD6319272.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            complement(2656..3381)
                     /locus_tag="KKO08_003113"
     CDS             complement(2656..3381)
                     /locus_tag="KKO08_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBD6319273.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            3499..3828
                     /locus_tag="KKO08_003114"
                     /pseudo
     CDS             3499..3828
                     /locus_tag="KKO08_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
     gene            complement(4634..5644)
                     /locus_tag="KKO08_003115"
     CDS             complement(4634..5644)
                     /locus_tag="KKO08_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD6319274.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            6385..7551
                     /gene="sopA"
                     /locus_tag="KKO08_003116"
     CDS             6385..7551
                     /gene="sopA"
                     /locus_tag="KKO08_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HBD6319275.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            7551..8522
                     /locus_tag="KKO08_003117"
     CDS             7551..8522
                     /locus_tag="KKO08_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HBD6319276.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(9556..9861)
                     /locus_tag="KKO08_003118"
     CDS             complement(9556..9861)
                     /locus_tag="KKO08_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319277.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(9915..10166)
                     /locus_tag="KKO08_003119"
     CDS             complement(9915..10166)
                     /locus_tag="KKO08_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBD6319278.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(10245..11516)
                     /locus_tag="KKO08_003120"
     CDS             complement(10245..11516)
                     /locus_tag="KKO08_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBD6319279.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(11516..11941)
                     /gene="umuD"
                     /locus_tag="KKO08_003121"
     CDS             complement(11516..11941)
                     /gene="umuD"
                     /locus_tag="KKO08_003121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBD6319280.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            12042..13262
                     /locus_tag="KKO08_003122"
                     /pseudo
     CDS             12042..13262
                     /locus_tag="KKO08_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
     gene            13717..14682
                     /locus_tag="KKO08_003123"
     CDS             13717..14682
                     /locus_tag="KKO08_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HBD6319281.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT         3445 a         3893 c         4006 g         3601 t
ORIGIN      
        1 gccagcgcgg gcagcatctg cggatgcagg ctgttttccg gttcgttgag ggcgataaag
       61 gccggtggac gcgggcttaa tagcgccacc gccaggcaca gaaaacgcag agtgccgtcg
      121 gagaattccg ccggctccag cggtctgctt agcccttcac gctgcatcat catccggaag
      181 cgtccgccgg tgttgtcgct gtaaaacacg cagccgggga aggcctggtc gaggatgcgc
      241 atcagcagca gctcgtcgcc aatttccacg atggtctgaa acgccgccgc caggtttgcg
      301 ccgtcgctgg ccagcactgg ggagcggaaa ccgacctgag gcgcgcgcat ggccgagccg
      361 attgatacag aaaactcctg ataaaagcgc cagttacgca gcgactcgcg catctgcgac
      421 acttccgggt aaagatgcgg ctcgccaagc tggccgaaca ccgattcgtt ctcatacagc
      481 gtgccgctgt gggtgacttt ctcgtgatgc acgttgttca gaaatacggc ctgattttta
      541 cgcttcatca gctgtgacga cgggcggcgg tattgaccac tcagccagat ggactcttct
      601 ttgatcaccg gatcgagttg aaactgtgag gggtaaggca gtttttcgac aaagcctacc
      661 tgcagctcgt attcgtaggt ttcggtttca acggccaaat tcatccgccg cagctggtcg
      721 ctgcgggttt tgcctgccca gaacactttt aaaatcccac cctcgttcgc cagtgcctgc
      781 gaaaactgcc cctgtgcggc gctgtgcatc aggtggatcg ccttgtagat attggatttt
      841 ccggtgccgt ttgggccaaa gacgatgttg agctgttcca gctccagcga catttcgcga
      901 atcgagcggt agttttgaat gtgcagggtg gtgatcacag gcagtccctt tttgcgttac
      961 aatcctgtac ataattacag tcttttgtgt ggaatgcaaa aaagagacga cagtgagggt
     1021 gtcgtctaaa cggggtactg ctttgggcct ctgtgggtca aacgggcctt cgcattttta
     1081 tcgtcaggtt actgccagac gcagacccaa atcatcagga tagggatcaa agtagttttg
     1141 cgtcatcaga taccggtcag ggtattcggc catgtagtgt ttcagtagcg tcaacggcgc
     1201 gaggatgggt agcagcccgt cgcgatagtg caggatcaca tcgcgcagct cgacgcgctg
     1261 gcggctgttc agctggttac ggaaatagcc ctggatatgc attagcacgt tggtatggtt
     1321 tttccgcgag gcgggctttt tcaggatggt catcagcttt tcgcggtacg ccacgaagaa
     1381 cgcgtccaga tcttcccatt catgcagcga cgcaacgaac gggccaatct cgcggtagcc
     1441 cgcctgatgg tgcgccagaa gctgcaattt atagcggctg tggaaatcca gcagcgcgcg
     1501 gcgcgtcagg cctttagcgc gcagcgtgtt cagctcgtgc agggcaaata cccgctcgat
     1561 aaagttttcc cgcagcaccg ggtcgtgcag ccgaccgtct tcctccacgg gcagccacgg
     1621 gtaggtttca agcagggcgg cggtgaacag cccgacaccc tccttgcggc cccggttgcc
     1681 gttttcatca tatagccgca cgcgttccat gccgcagctt ggcgatttcg cgcagacgat
     1741 aaatcccgac agatcgccga tttttggcag atagtccgcc gtaaagtcgg ccattttctg
     1801 cgttatatca tcatggggcg gcttgctgaa gcggagctgc gtttcgccgc tgtccgtcag
     1861 cgtcaggcgt atggccgggc gtggcgtcgg caggccaatc gccatttccg ggcagacggg
     1921 cctgaaactc acccactggg ccagttcatc catgacaaag cccatacgct tgtgtccgcc
     1981 gtcgaagcga acggcagacc cggtcaaaca tccgctaata ccgagcacag gttttgttgt
     2041 catggtgtgg tcctcccgtt cttctcttta aaacttacac aacataacta atttgtccaa
     2101 gtattagcgg aattattttt tatttgcctt taaaatcaac gtttcttaac tgcccttgta
     2161 agttgaataa ccgtattggc tcagcaggag agggatgtgc agcttttcgt tggtccgggt
     2221 tacggtgaac agcaccggga tctccgggaa gaaggagtcc agcttcttcc cgtggaagta
     2281 atctgcggtt ttaaaggcca ctttatagac gccgggctgc atatcctgat cctccggata
     2341 aagcgatttg atgcggccgt catggtcagt tttgccgctg gcaattgggg tccatttgtc
     2401 ctgctgctgt ttttccagcg tgacggtaac gtccgatggc ggcatcccgg tttgctggtc
     2461 aagaatgtgt acgctcagcg tgccggcagg ggcgctaaag gcggcagggg caaaggccag
     2521 ggtggtcagc atcagcagag aggcagtttt tttcattgtg ttttcctgtg gggttgtcgg
     2581 tcacaggaaa acgttagcat ttgtggcgcg ggaaaatatt caacttttcg tgaagattcc
     2641 cgcaggagaa aatgattaga ggataatcac ttcaagcgat acctgctgct gccactgctc
     2701 gatctgcttt ttgcgcgcgg cggtgagttt accgcgcgtg accagaagcc agtggcggtc
     2761 cgggaaaatc tccggggcca gcgtggcggg cggcgtgggt aaaatatcga tacggtggcc
     2821 ttgcccggtg cgggttaagg cttcgagcca gacttcgcac gggtcattca ggttccagcc
     2881 gctgagcagg atgttgtcgc ccggcgcttt tttatcgcct tccaagcaaa acgaggtgta
     2941 ggcgatgatg atgccgtcga ggatctcccg cagggtcatg gcggcggcga cgtttgccga
     3001 cacctgactg cgcagcggac gcagcacgtt cgcgaccagt tctggtcttg ggtactcgcg
     3061 tcctgcgtcg taaatcatct gacgcaggga ctcgattttg ccgtcctgca ggcgctgtaa
     3121 catgctctgc tgcagcgtga gccagttgtt ggtcaggcgc gacgtagggc gctccagcag
     3181 cggtttgacc tggccaatcg ggacgccttt ttttacccag tcgaggattt ttagcgcctg
     3241 ctggacgtcc tcatcgctgt actggcggtg gccgccctcc gtgcgctgag gcttgagtaa
     3301 gccgtagcgc cgctgccagg cccgcagcgt ggtggcggtg atcccgctga gtctggcgaa
     3361 ttcgccgatg gagtaagcca tgtccgtgtc cagagttgag gaatactctc aatatactac
     3421 gaattttcgc ggatgggatg aagcgccgcc gcggtgcact acacttaatt tccaaccgtg
     3481 caaatgaaag gaattctcat gaagctatgg cctgttgtga ccggtgttgc cattgccctg
     3541 acgctggtag cctgtaaatc ccctacccca ccgaagggcg tgcagcccat ttccggtttt
     3601 gacgccagcc gctatcttgg caaatggtac gaagtcgctc gcctggaaaa ccgctttgaa
     3661 cgcggcctag agcaggttac cgccacctac ggcaagcgca gcgacggcgg catcagcgtg
     3721 cttaaccgcg gctacgaccc tgtgaagaac aagtggaatg agagcgaggg taaagcctac
     3781 tttaccggcg aaccgaccac ggccgcgctg aaggtgtcct tcttggggtc agtttggata
     3841 tagagaatta ttgtacggta agcctgtttt ttgaacaggc ttacctgtag caatagggtg
     3901 tactcccgcg ctatacagcg ttttcagggg attttgtctt aatcggataa cctaaatgcg
     3961 atacagcaag agcgacaacc taaaagcgat agccaatgac gataaatcaa tcacttacgt
     4021 atccgaattg gttaaataat gtgcttaacg tacaaaaatt tccgatctcc aaactgaccc
     4081 ctaaataggc tgattcgatg tgtttgcggg aaaaaaatcg gcccagatcc gcgaaatttt
     4141 aatcagcgag tcagcttggg aagaaataac ctgcttattc gcaccttccc tagccaacac
     4201 ctttctgcca ttcggctccc gtttcctgat cgcatttaac agtgtgaagc tggcgccggg
     4261 ttatacagac agctgcagtc tcatcctgcc gggctaaacc tgcagtacac aggggaacgg
     4321 ataccgtcag gtaacatgac cgtctcagca cccgattgag attttcgtaa gggatataga
     4381 gcgttactga aaaattgact gtggcatgtt atcccccatg gaatggtgaa cagaccaggg
     4441 attaccccca ttaaacccgc attccgtagt caatatctgc gtatcatgac ccgaaatcac
     4501 atatgacagt caatattgtt cgatccgggc agacatgata gagaataaat attgactgcg
     4561 gtatgcgtga tgcatatcac agtcaatgga atgtccttag cggcatacgg cagtcatgat
     4621 atgcatatcg cagtcaattt tctggcttca tcgctgagag ggcttcagcc agtttaagag
     4681 gatcgatgcc agcagccttc agcgccttaa tgacttcgtc ataattttgc tctggcgcct
     4741 tctcttcttc ctctttgcgc ggaaccttca caggactact gatgagtttg gggttgcgat
     4801 aatgcacgct gaaataggtg gctcggccac gtttaaactc ggtgtaatcc agataaccaa
     4861 tatcacgcag ctgctccatc gccttacgca ccgtgtggtt ctgtgtatag acatttgagg
     4921 tgagattcag ccggtcacgc atccgtttca tggaaatagg tgcaggattc tggggaagac
     4981 tttcgatgta ggtataaagc gcctgggctg attccttgcg ctgcagggcg tcaatggctt
     5041 tcagccgcag cagtaccctt ctgtccatgt ggtaaagctc aaacagctta ggttcagcct
     5101 tgatctcaac gatatcctcg ttgatgtcat aataggctga ctgcaccaga tgggtggtcc
     5161 atcctttggt ctcgctctgg aacttcagcg taaccgaggc gagtttaaac agggaattac
     5221 tgatgcgatc gcgcattttt ttgtttgaac gacgggagtc aaatccgcac atcttaacga
     5281 attcgacgaa cgacagttcc agggtgtcac tttttacccc atactccgac atggagcgaa
     5341 ttatccccag ccagacttta aaatccgtgt ccatatcaag acgcgaaccg gtgatcttaa
     5401 cgtcagtgta tccttcgctt ttggcaatgg ccagctgtac aagctcctca gtcgcgtctg
     5461 taacgttttt ccggttcgcc ttgctcttcc ctgttgattt cagagttggg acgaagagac
     5521 cgagtctcat gagcgctact ggctgcacgg tactggtgct gttgacatcc agcttaacaa
     5581 cttcaccggt tgtcttatca acttcctgaa ggttcagaag taagctatta ttttcgctcg
     5641 tcatcttgat cctcaacggt tatccacaaa aggatgtagt aatacgtatc gcagtcaacg
     5701 gatatacata cctcagtaaa taccgttgca tatcacagta ttagattatg catataacag
     5761 tcaacatttt acattccgca gtcgatccag acagacgtta aagccagtta cagcgcatgc
     5821 tggagcgatc cggggatctc ttttggatct cactatgatc tccttgggat caattattgg
     5881 atcgagacca tgaataaccc agatacaagg ccggaagcgc cacaccagat atccagaccc
     5941 ctggcctgat tttcctgaat acgctctgga agattgagat ctacaggtac aacaatgaaa
     6001 cattgactgc ggtatgctga gtcgtctttc ctacagatgc ggtaagcatc ataccatagt
     6061 caatatatta cataccgcag tcaatttcat tataactggc tgatcccgag tcatctttag
     6121 caacccgacg tacgtgcaat ctgtgagttc tgtgattttc cgcttcctcg ccgctttctc
     6181 actgtgtgag atcgcttact gcggcgagcg gcagtgatta atattgactg cggtatgtac
     6241 tcttttgtaa tcccctgcgt tctttcatta cccaatgtgg ttcactttgc actttttcgt
     6301 gtgctttgcg attttattgt tgctttgcaa taaattccaa gatttaataa aataatgcaa
     6361 agtgatgatt aaaggatgtt cagtatggga ctcatggata cactcaacca gtgcataagt
     6421 gctggtcatg aaatgacgaa agcaatcgcc attgcacagt ttaatgatga cagtccggaa
     6481 gcccggaaaa taacccggcg ctggaggatt ggtgaagcgg cagatttagt tggcgtgtca
     6541 tctcaggcta tcagggatgc cgagaaagct ggccggctgc cgcaccctga tatggaaacc
     6601 cgtgggagag ttgagcaacg cgttggttat accatcgaac aaatcaatca catgcgggat
     6661 gtatttggta cacgactgcg ccgtgctgaa gatgcgtttc cgccggtgat tggcgttgca
     6721 gctcacaaag gtggtgttta caaaacctca gtatccgttc acctggctca ggatcttgct
     6781 ctgaaaggac tccgtgttct gcttgttgaa ggcaatgacc cacagggaac tgcttctatg
     6841 tatcacggat gggtgcccga tcttcatatt catgctgagg acactctcct gcccttttat
     6901 cttggggaaa aggatgacgc cagctacgct ataaagccca cctgctggcc tggccttgat
     6961 atcatcccgt cctgtctggc actgcaccgc attgaaaccg agctgatggg gaaatttgac
     7021 gaaggcaaat tacctgctga cccgcacctg atgctccgtc tggccattga aactgtcgct
     7081 catgattatg acgtgatagt tatcgacagt gcacccaacc ttggtatcgg tacgataaat
     7141 gttgtatgcg ctgctgatgt tctgattgtc cctactccgg cagagctgtt cgactacacc
     7201 tccgcactgc agtttttcga tatgcttcgt gacctgctta agaacgtgga tcttaaaggc
     7261 ttcgagccag atgtccgtat tctgcttacc aaatacagta ataataatgg ctcacagtcc
     7321 ccgtggatgg aagaacaaat tcgggatgcc tggggaagca tggtccttaa aaatgttgtg
     7381 cgcgaaacgg atgaagtcgg taaaggtcag atccgtatga gaaccgtttt tgaacaggct
     7441 attgatcaac gttcttcaac cggtgcctgg cgtaatgccc tctctatctg ggagcctgtt
     7501 tgcaatgaga tttttgatcg tctgattaaa ccacgctggg agattagata atgaagcgtg
     7561 ctcctgtcat tccaagacat accacacata gtcaatcgac tgaagatact tcatcaccgg
     7621 cgccggccgc gccgatggtg gattcattaa ttgcgcgcgt gggtgctatg gcccgcggta
     7681 atgcaatctc tcttccggta tgtggacggg aagtgaaatt tacccttgag gtgctccggg
     7741 gagactctgt tgagagcgct tcacgcgtat ggtcaggcaa tgagcgcgat caggaactac
     7801 ttaccgaaga tgctctggat gatctcattc cttcattttt actgaccggt cagcagactc
     7861 cagctttcgg ccgccgggtt tctgatgtca tagaaattgc cgacggcagc cgtcgccgta
     7921 aagccgcaat cctgacggaa agtgattatc gcgttctggt tggtgaactg gacgatgagc
     7981 agatggctgc attgtcccga ctgggtaacg attatcggcc gacgagtgct tatgaacgtg
     8041 gcctgcgtta tacaagccgg ctgcagaatg agtttgcagg aaatatttct gcgcttgccg
     8101 atgcggaaaa catctctcgt aagatcatta cccgctgtat caatacggcc aaattgccta
     8161 aatccgttgt tgccctgttt gcacatcctg gagaactgtc tgcccggtca ggtgaagccc
     8221 ttcagaaggc ttttgcagat aaagaagaat tactgaagca gcaggctgag actctccacg
     8281 atcagaagaa agcgggacta atctttgaag ctgaagaggt cattagtctt ttaacatccg
     8341 tactgaaaca atctcccgca tcaagagtta atctgagctc acgtcatcag tttgctccag
     8401 gggcaacagc attgtataag ggcgataaaa tggtgcttaa cctggatagg tcccgcattc
     8461 ctgcggagtg tatagagaaa atagaagcca ttcttaagga gcttgagaaa ccaggagtct
     8521 gatgcggaca cgctttagtc tacggtgatc tccctttact tcatttcctt tctcacaggc
     8581 cagaaaacat aactggcctg aatattctct ctgggaccac ggtcccacct gtatcgccgc
     8641 tcaggttatc tccctgggac cacggtccca cctgcgtcgt cggtcagctt atctgcctgg
     8701 gaccacggtc ccacctgtat cgccggtcag gttatctgcc tgggaccacg gtcccacctg
     8761 catcgccggt cagcttatct gcctgggacc acggtcccac ctgtatcgcc ggtcaggtta
     8821 tctgcctggg accacggtcc cacctgcatc gtctgtcagc ttatctgcct gggaccacgg
     8881 tcccacctgt atcgccggtc aggttatctg cctgggacca cggtcccacc tgcatcgtct
     8941 gtcagcttat ctgcctggga ccacggtccc acctgtatcg ccggtcaggt tatctgtctg
     9001 ggaccacggt gccacctgta tcgccggtca ggttatctgc ctgggaccac ggtcccacct
     9061 gcatcgtcgg cacattatta gttagggaca acggttcaat caatgcctgt cacgaacagg
     9121 tattggcttg tcgccagcaa cccgttgagc ggagtaatca cgcagcagga cggctgttac
     9181 aggcgatggt gaaagctctg ttgggatctg aacgaagcat gttgagtgaa atttgcaaaa
     9241 tttcgcagag tatgcggctt catctttaaa tttccgccag catttttaaa gccagctacc
     9301 gacgctgcgc gttggccggc ccttcgtccg gcgggtaacc agtaccggcc gatcttacga
     9361 ccctggatga acatcatccc gtcagcagcg tctccactgt gatgtcgttt ttcacactgt
     9421 ccgatcacaa aatagaacgg gcagggacaa catatggtag gaaaagcagc tgccagagac
     9481 acaatatatt gattacccac agtaacgggg gataaccatg tgggtaacat attgaaagtg
     9541 caacacgtta tctgattaat tgactttgta aacgccgctg aaccatgaaa aaaaaccggg
     9601 ggaatcatca cagcgcataa acataccgtt ttgtttttgg gtgacggcaa ggaggcccct
     9661 tttatcacca cataccttgt caaaggtttc catttctatc cttatcaggc tcttgtcgtt
     9721 attttcagga gagatccgga gatgttttgt cagccagaca ggataggtat cagcgttacg
     9781 ccagtactgc aattcaatca ctgaagccat tccccagaca gcgaagtata agaaaaccac
     9841 acctgcgatt ttgctaccca tattgttcct taacattggc ttaatacatt ccttgctcct
     9901 cgcaccggag cacatcacag atactgattg agatccactt cacgcagcgg ctggttttga
     9961 cgttctgtta tcgtttgcac caggtctttt tcgtccagct catcgagcat ttgttcatac
    10021 aaagccgcgg gcacacaata aaaaacaggg cggttacggt caattatctc gaccgggtag
    10081 ccgccgcggg cactggcggt ggccaccgga tcgtttttaa gttcgctgac gctggcagtc
    10141 acatcacaca gaatgatatt tgtcatggtt cccccctgag acaagtcacc tgtatgatcg
    10201 gtaaagtaag ctctggcggc aagtcccttc tgtcatttca acggtcagaa acgggcaacc
    10261 ggcagctcct tccagcgtgt ggtatatgcc ggtgacagca tctcccgttt catctgccag
    10321 tccggcgcaa taccccgtcc ggcaaaccag actttcccga gtccggaatg attaatgccg
    10381 tcgagtacct tcatcagcgc atcgctgtgc ggccgtggct gcacgtcatc gaacagattc
    10441 agctgcgcga cgccgttcgg actgaaatca ttgagcatta tccccgcttt tgcgtagcgg
    10501 tggccatcga gccatatcct gtccagagac ctgaccgctg ccgcgataat gtcccgggtg
    10561 tcacgggtag gggtgttcag tttttccgtg gccacgttac cgtaatacgg ctcattaacc
    10621 gcaaaagggg atgtcttgat gaaggtgctg atatgccggc agtactgtcg ctcaccgcgc
    10681 aacttttccg ccgcgcgctc ggcatactgg cagacggcct ggcgcatttc tccgtaggtg
    10741 gtgatccgct gcccgaagct gcgactgcag acaatctgct gtttcggcgg cggcgcttcc
    10801 tccagcgaaa tgcaactctc gccgttcagc tcacgtaccg tgcgctccag cactacgttg
    10861 aagtttttcc ggataaatgt agggttcgtc agcgacagct gcagggcggt ggtgatcccc
    10921 ataacgtgca gctttttcgc tatccggttc cccacgcccc agatttcttc caccggctgc
    10981 aggctgagta attttgccgt tctgcgtgga ttttcgggcg acagcgccag caccccactg
    11041 aattgtggcc attcctttga cgcccactgc gccgatttgg ccagtgtttt tgtggcgccg
    11101 aagccgacac cgatcgtcag tccggttcca ctgagaacat gcccacgcag ctgcctgcca
    11161 aaatcctcca gatccataca gtggctaatt ccccgggcat ccaggaaaca ttcgtcaatt
    11221 gagtattgtt cgacgcgggg cgacagctct tccagcaggg cgaccacacg gttactcagg
    11281 ctcgcgtaga gctcgtagtt gctggaaaaa acgtacagtt tttcaggaaa ctgcgcctct
    11341 ttgagctgga accagggggt tcccatcttt atacccagcc gtttggcttc agcactccgg
    11401 gcaataacgc agccatcgtt gttactgaga acgacgacag gtttgccgcg caaatccggc
    11461 ctgaaaacct tctcgcaact ggcgtagaag gagttgacgt ctgccagggc gaacatcagt
    11521 ctgccctccg ggttttatga atgaaggccg tcaccacgcc gaaaatttgc agagtttccg
    11581 gatccgggta cagggaaggg aagtccgggt tcatcgcctg cagggctggc cgcggcgtca
    11641 gaagcaggcg ttttaccgta aattcaccct caatttccgc gataacaata tccccgtgcc
    11701 gcggctgttc tgctttatcc acgaccatca gatcgccgga atagaggcca atatcagtca
    11761 ttgagttgcc aatcgctctg acaaaatagg tggagtggcg ccggtgaatg cagtactcat
    11821 tcagatccag ttcagactca gtatagtttg ctgccggtga cggaaaaccc gcagggcatc
    11881 gctctgtgaa caaaggaatt tttactatgc tggcttctga tactgcagga atgaacttca
    11941 tcgttttttc cctcgcttaa aactgtatat gcatgggtct tcccttgttg tggtggctga
    12001 aggcatgata atggtgtatt taatcgccag aggtcaccgc catggacgaa aagtccctct
    12061 acgctcatat tctcaacctg tccgatccgt ggcaggtaaa gtccctttct ctcgatgaaa
    12121 atgccggttc tgttactgtc actattgaga tcgctgaaaa cacccggcta gcctgtccga
    12181 cctgcggtaa atcctgttct gttcacgatc accgtcatcg taaatggcgc catcttgata
    12241 cctgccagtt caccactatt gttgaagccg atgttccacg aattatgtgt ccggagcatg
    12301 gctgcctgac gttgcctgtt ccgtgggctg gccccggaag ccggtatacg ttgctattcg
    12361 aatcgttcgt tctctcatgg ctgaaaatca gcaccgttga tgctgtcagg aagcaactta
    12421 agctcagttg gaatgcggtt gacggcatta tgacccgggc agttaagcga ggtcttgccc
    12481 ggataaaaaa gccattatcc gcccgtcata tgaatgtgga tgaggtcgcc tttaaaaaag
    12541 gacatcgtta cataacggtg atctccgatc gcgatggtcg ggcgctggcc ttaacggatg
    12601 atcgcggcac agagagtctt gccggctatc ttcgcacgct cactgatggg cagttgctgg
    12661 ctatcaaaac gctctcaatg gacatgaacg cgggctatat aagagcagcg cgtatccact
    12721 tacccagtgc ggttgagaaa atcgcctttg accgcttcca tgtggcgaag caactgggcg
    12781 aggtagttga taaaacccgt cagaatgaac atccgcacct ccctgttgaa agccgacacc
    12841 aggcaaaagg aacccgcttc ctgtggcagt acagcgataa gtggatgacc gaatcccggc
    12901 aggaaaagct gatgtggctg cgtgcacaga tgaagctgac gagccagtgc tgggcgctga
    12961 aagagctggc aaaggatatc tggaacaggc catggagcga ggaaagacgg agtgactggc
    13021 agagatggtt ggcgctggcg gctaacagtg acgttcccat gatgaaaaat gccgcgaaaa
    13081 cgataggaaa aaggctgtag gggatcctga atgcgatgcg acacagtgtc tcaaacggaa
    13141 atgcggaggc acttaacagc aagatcaggc tgctgaggat aaaagccagg ggataccgaa
    13201 accgggagcg ctttaaactg ggggtgatgt tccactacga aaagctgaat atggcgttct
    13261 gagccttccc accatgatcg gggaagaccc atatgcatac agtataaggg gcggtgaaat
    13321 ttctgcaagt gatgggggga tagccggtca gaacgtcccc gaaaacaaac cgcccccggt
    13381 ttcaggaagg gcgaactgac cggcagaggc agtgtaacac cgcatgtcac aaaggcagca
    13441 ctgacgtcgt cagtgctttt tttacccgtc ctcccggacg ctgaagggca gtttcacccg
    13501 tgcagctaaa ggggcggtaa gtccgggtgt gcacatcgtc attttctcct gtgatgcctt
    13561 tctcctgcgt atcagcttcc ggtaatcgtc ccccgtcaca acctggacag caagggtaaa
    13621 tggcacgctg cgcgcccttg ctgaccagaa cgactccggg caggccgaac cgtcaggcga
    13681 tacgaagacg aaattcacac tgacggagaa acagccatga cgatgaacct gcacactcag
    13741 acaaccgccc ctaaagcttc acaggcgacc agcgtatccc cgctggagca gtcaggctcc
    13801 tcaaaaacga aattttccag gggcaaaagc aaagcccgtg ctggcgcagt caaaacagac
    13861 ctttaccaga cagtaacgga cagcatcatt gcagccctgg aaagcggcgt taagccgtgg
    13921 gtgtgtccgt gggttcgcaa cggcgcagcg gtgggattac cggccaactt cagcaccggc
    13981 acggcataca gcggaattaa tatcatgctg ctgtggtgta gtgcggcaaa acagggtttt
    14041 caggacgagc gctggctgac ctacaaacag gcgcaggagc tgggcggcca ggttcgtaaa
    14101 ggtgaacacg gcacgacggc catcttttac aagacgctgg agaaagagga cgaggacggg
    14161 gaaatcgaaa aaatcccgat gctgaaagcc tttaccgtgt ttaacgtgga gcaaatcgac
    14221 ggcctgagca tcgagagcgt gccgcagccg gttgccggtt tcgatccgct gccgcaggcg
    14281 gaagcactca tgatccggag cggggcaaaa atcaccgagc agggcgtaaa ggcgttctat
    14341 cgtccggcca ccgatgaaat cgtcctgccg gagcggttcc gcttcgcgga cgcggcgaac
    14401 ttctacgcca ccggcctgca tgagctggtt cactggacgg gtgcagcgtc ccgcctcaat
    14461 cgtgaaaaag gcaataagtt tggctctgag gcgtatgcgt ttgaggaact gatcgccgag
    14521 ctgggcagcg cgtttctgtt ggcggatttg gggattacgg gagaggtgca gcatgaaagc
    14581 tatatcgcgt cctggctgaa agcgctgaag ggagacaagc gctacatctt taaagcggcg
    14641 gcagcggcct caaaagcgca ccgctggctg atggactgct gacagctgga gccctgcggt
    14701 gcagggcttc agaatttttg aaagagagag aatcagggag ggaattatat aagtcactgt
    14761 tttttagaat tctgtaacgc ggccggcctc agctgcagga cgtcctgcag ctgaggccgg
    14821 cacggaaaaa taaaccggac agattcaaaa aaaatgaacc ctttttcagg cggtagccgt
    14881 tacgtatccg ggatacgtaa cggcgcgatg tcttacgtaa aaaatgacgt aacggctttc
    14941 gccgc
//
LOCUS       DAECNO010000095        14920 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.095, whole genome shotgun sequence.
ACCESSION   DAECNO010000095 DAECNO010000000
VERSION     DAECNO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.095"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="KKO08_003124"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="KKO08_003124"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBD6319282.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KKO08_003125"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="KKO08_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBD6319283.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KKO08_003126"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="KKO08_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBD6319284.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="KKO08_003127"
     CDS             2977..3060
                     /locus_tag="KKO08_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBD6319285.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KKO08_003128"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="KKO08_003128"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBD6319286.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KKO08_003129"
     CDS             complement(4538..5380)
                     /gene="rlmJ"
                     /locus_tag="KKO08_003129"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBD6319287.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="KKO08_003130"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="KKO08_003130"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBD6319288.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="KKO08_003131"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="KKO08_003131"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBD6319289.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KKO08_003132"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="KKO08_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KKO08_003133"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="KKO08_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBD6319290.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="KKO08_003134"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="KKO08_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBD6319291.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KKO08_003135"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="KKO08_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBD6319292.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="KKO08_003136"
     CDS             13186..14385
                     /locus_tag="KKO08_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBD6319293.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3712 a         3789 c         3929 g         3490 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag gcgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catttttagg taatgctgcc aacttactga
    14881 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt
//
LOCUS       DAECNO010000096        14844 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.096, whole genome shotgun sequence.
ACCESSION   DAECNO010000096 DAECNO010000000
VERSION     DAECNO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.096"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(115..381)
                     /locus_tag="KKO08_003137"
     CDS             complement(115..381)
                     /locus_tag="KKO08_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBD6319294.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="KKO08_003138"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="KKO08_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBD6319295.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="KKO08_003139"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="KKO08_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="KKO08_003140"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="KKO08_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBD6319296.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="KKO08_003141"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="KKO08_003141"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBD6319297.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="KKO08_003142"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="KKO08_003142"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBD6319298.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="KKO08_003143"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="KKO08_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBD6319299.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="KKO08_003144"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="KKO08_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="KKO08_003145"
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="KKO08_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBD6319300.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12643..14097)
                     /locus_tag="KKO08_003146"
     CDS             complement(12643..14097)
                     /locus_tag="KKO08_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBD6319301.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..14489)
                     /locus_tag="KKO08_003147"
                     /pseudo
     CDS             complement(14094..14489)
                     /locus_tag="KKO08_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            14602..14790
                     /locus_tag="KKO08_003148"
     CDS             14602..14790
                     /locus_tag="KKO08_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319302.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT         3937 a         3764 c         3509 g         3634 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaactgc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaagatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatccgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tgcaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc acgaaccacg gcagcctgac ggctgcaaat tgaaaaccgc
    14521 ccctgttcgt cctgttgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
    14581 ttattaagga ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct
    14641 gttggtgctg agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg
    14701 gacccaccac ctgcgatggt cgcggcctcc atcctggagc gccgtggcga cacagcagcc
    14761 cttacccggc tacaggatac cgggggttaa tccgtcctca tgttgtccgc ttttactccc
    14821 cgcccgctga aacgtctgtt cacg
//
LOCUS       DAECNO010000097        14621 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.097, whole genome shotgun sequence.
ACCESSION   DAECNO010000097 DAECNO010000000
VERSION     DAECNO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.097"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(400..645)
                     /locus_tag="KKO08_003149"
     CDS             complement(400..645)
                     /locus_tag="KKO08_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319303.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1290..1365)
                     /locus_tag="KKO08_003150"
     tRNA            complement(1290..1365)
                     /locus_tag="KKO08_003150"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1330..1332),aa:Phe,seq:gaa)
     gene            complement(1471..2178)
                     /locus_tag="KKO08_003151"
     CDS             complement(1471..2178)
                     /locus_tag="KKO08_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBD6319304.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2576..4711
                     /gene="speC"
                     /locus_tag="KKO08_003152"
     CDS             2576..4711
                     /gene="speC"
                     /locus_tag="KKO08_003152"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBD6319305.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4760..6016)
                     /gene="nupG"
                     /locus_tag="KKO08_003153"
     CDS             complement(4760..6016)
                     /gene="nupG"
                     /locus_tag="KKO08_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBD6319306.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6218..7297)
                     /gene="mltC"
                     /locus_tag="KKO08_003154"
     CDS             complement(6218..7297)
                     /gene="mltC"
                     /locus_tag="KKO08_003154"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBD6319307.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7362..7637)
                     /locus_tag="KKO08_003155"
     CDS             complement(7362..7637)
                     /locus_tag="KKO08_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBD6319308.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7665..8717)
                     /gene="mutY"
                     /locus_tag="KKO08_003156"
     CDS             complement(7665..8717)
                     /gene="mutY"
                     /locus_tag="KKO08_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBD6319309.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8878..9597
                     /gene="trmB"
                     /locus_tag="KKO08_003157"
     CDS             8878..9597
                     /gene="trmB"
                     /locus_tag="KKO08_003157"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBD6319310.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9597..9923
                     /locus_tag="KKO08_003158"
     CDS             9597..9923
                     /locus_tag="KKO08_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBD6319311.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10107..10826
                     /locus_tag="KKO08_003159"
     CDS             10107..10826
                     /locus_tag="KKO08_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBD6319312.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11002..12048
                     /gene="ansB"
                     /locus_tag="KKO08_003160"
     CDS             11002..12048
                     /gene="ansB"
                     /locus_tag="KKO08_003160"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBD6319313.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12165..13126
                     /locus_tag="KKO08_003161"
                     /pseudo
     CDS             12165..13126
                     /locus_tag="KKO08_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(13227..14540)
                     /locus_tag="KKO08_003162"
     CDS             complement(13227..14540)
                     /locus_tag="KKO08_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBD6319314.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPK
                     LILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLL
                     IVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGC
                     SISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3826 a         3502 c         3756 g         3537 t
ORIGIN      
        1 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
       61 caggctgagg agtccccaga aaagaagaca ggcatagagt tttgtgatct actgattgcg
      121 ctaacaaatt caatgagatc acctctatgc ctgcccgtta agtatgtcag gcttttttcc
      181 gtaatgctct aacttccacg cattggtttc tgacatcaaa taaggctatg actttctctc
      241 ccttttaatc tcattctgat ctaaaaccat atcttaaaaa aatgcatggg tattttaatt
      301 attttgatgt tgatctaacg cactcaagga ccattttatt accgattatc cattctattt
      361 aactgttgcg atataaatgt ttgttttagg tgctttaatt cagtacaagt catcaaaccg
      421 taccagaccc tgactgtcag tgacaccgac aggtgggggc ggcggtgtgg ggtcaaactg
      481 agtgtgtctg cctctggccc acagagccag ctctgccgcc attgcccgtg gatgatactc
      541 cagctgcacg ttgagaccgg aaactgggtt gttgttctca tccacgaact gcagcgccag
      601 ccagtgcatc cgctgcttac cgcaggcgac ttctttgtac ttcattttgt gactcaggca
      661 ggtgactgtc gtgttgcctg ttgcgtccat tttgcggtta aaccctttta attcaatttg
      721 ttaaattgct ttccaccttt tgcggggcgc gacttaacca aaatattcac tgttatcata
      781 atatgcaatg ggctggaggt tgcaggtcat ttagggattt tctgtaagcc ccgttttgca
      841 gaacgttcta tctccgattg agatcctgtc tctcggtatt accggtagtc tggccataat
      901 cccctatgga tcatattgat atagtaggca taacacccaa aagttgacta aagctaaatc
      961 aatcagagtg aaaaatgttc ggtttgatgt agggggggac tgaggagggg ccaacgcagg
     1021 tggtatgcgt tttacttcag accggacaaa gaccggatgt atccgtgcag taatgaatcc
     1081 gttagccagg tgctcatgtg gaaatagtgt ctattgggga tttgaagttt gacagaaaag
     1141 gccacagtga ccagaagggg gctatttaga agaaatgttc aggctggatg gaagaggtaa
     1201 agcagtgaca tcagcattca aagtgaccgg atatctacca gaaccccaga aagcaaaaaa
     1261 ccagcccgta ggctggttct tctaaatagt ggtgcccgga ctcggaatcg aaccaaggac
     1321 acggggattt tcaatcccct gctctaccga ctgagctatc cgggcaacgg ggcgcattaa
     1381 acctgattcg cctcgtctcg tcaatcaaat ttcttcaatt tactgcagac tgcacaatct
     1441 atcatcactt tgccgctatt gcacgcattg tcaggcaaac caggcggtcc aggcggcgga
     1501 aagcggcatt gccagaagga gtgctggaag catgttgacc accgggaaca ttttaatgcc
     1561 acagatgcgt aatccggttg ccagcagcaa taaaccgcct acggcgctga agtctgccat
     1621 catcgacggt gtgatcagcg gtaatatcag cgcggcagcc cacgccagcg ttaactggat
     1681 gatcagtaat gggatactaa ttaccgacac cgcaataccc agcgagcagg cgaagatcat
     1741 cgccgtaaag aaatcaagaa atgacttggc gattaaaata ctcggatcgc cggtcatccc
     1801 ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac
     1861 ataattctga ataaaagatt catgcgctgg cttcttacgt gagtgacgaa acagattttg
     1921 tgctttggcg actgctgtat tgacaccttt ctccagcaaa caaatttcgc caattagagc
     1981 gccaagtagc gtagctaaaa ccatcgctgg aaggttggcg catttcacaa ccagtaaaat
     2041 accaatcccc agcgatgcca gaccaaaaat agatgtcatg gagacgcgga tacgttccgg
     2101 taagcgttgg ctgagcagtg cgccgagaac gccacccagt aagactgcac tagcgttgat
     2161 aaaagggccg atgaccacaa gagttcctgt tagctgtatt gtttgatttt cttattatga
     2221 actttttgcc ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac gccttagcga
     2281 aaggtgccat gattgcgcga attttctcct ctctgtacgg agtttgcccg atgcacgcca
     2341 tctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa cgtttccctt tctatgttac
     2401 tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat cagagcgttt
     2461 ctgcttttac tgatgtctgg cggtcggagc tggtgaccag tttgacccat atctcatggg
     2521 gcagggtttt ccaccttgtc cggtattctt acttccccga aacgggtttg cgcttatgaa
     2581 atcaatgaat attgccgcca gtagtgaagt ggtatcccga ctttcttctc atcgccgcgt
     2641 ggtggcgttg ggagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga
     2701 tagtcgcagt ggcattcttg cgttgcttaa gcgcaccggt tttcatctac cggtgttttt
     2761 gtattccgaa catgctgttg aattacctgc gggcgttacg gcggtaatca acggcaacga
     2821 gcagcagtgg ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc
     2881 gttttatgac acgctgacgc agtacgttga gatgggcaac agcacctttg cttgccctgg
     2941 acatcaacat ggtgcgttct ttaaaaagca tcctgccgga cgccattttt acgatttctt
     3001 tggtgagaac gtctttcgcg ccgatatgtg taacgctgac gtaaaattgg gcgatctgct
     3061 tattcatgaa ggatcggcga aagatgcgca gaaattcgca gccaaagtct ttcatgccga
     3121 taaaacctat tttgtgctga acggcacatc ggcagcgaat aaagtggtga cgaatgcgct
     3181 gttaacgcgt ggcgatctgg tgctcttcga ccgtaacaac cataagtcga atcatcacgg
     3241 cgcgctgatt caggcgggga cgacgccggt ctatctggaa gcttcacgta acccgtttgg
     3301 tttcattggc ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg
     3361 cgacgttgcg ccagaaaaag ccgacctgcc gcgcccgttt cgcctgacga ttattcagct
     3421 gggaacctat gacggcactg tctataacgc ccgtcaggtg atcgatacca ttgggcatct
     3481 gtgtgattac attctgtttg attccgcgtg ggtcggttac gaacagttta tcccgatgat
     3541 ggcggatagc tcgccgctgc tgttagaact taacgaaaac gatccgggga tttttgtgac
     3601 ccagttggtg cacaaacagc aggctggatt ctcacagacg tcgcagatcc ataaaaaaga
     3661 taaccatatc cgcggacagg cgcgtttttg cccgcataag cggttgaata acgcctttat
     3721 gctccatgct tctaccagcc ctttctatcc gctgtttgct gcactggatg ttaacgccaa
     3781 aattcatgaa ggggagagtg ggcgtcggct gtgggctgag tgcgttgagt tgggtattga
     3841 gtcgcgcaag gctattcttg cgcgctgtaa gctgttccgc ccgtttatcc cgcccgttgt
     3901 tgatggcaaa ttgtggcagg attatccgac atcagtgtta gccagcgacc gccgtttttt
     3961 cagttttgag ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt
     4021 tgttgatccg tgcaagctgt tactcactac accaggtatc aatgccgaaa ccggcgaata
     4081 tagcgacttt ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga acggcattgt
     4141 gccggagaag tgcgatctca actccattct gttcttatta actccggcgg aaagccacga
     4201 gaagctggca caactggtgg cgatgctggc gcaatttgaa cagcatattg aggatgactc
     4261 gtcgctggct gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta
     4321 caccctgcgc cagttgtgtc aggagatgca cgatctgtat gtcagtttcg acgtcaaaga
     4381 cctacaaaaa gcgatgttcc gccagcagag tttcccgtca gtggtgatga acccccagga
     4441 tgcgcatagc gcttatattc gcggtgaagt ggagttggtg cggattcgtg atgccgaagg
     4501 gcgaattgcg gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg
     4561 ggaagtctgg ggtggggcgg ttcaacgtta tttccttgcg ctggaagaag gcgtgaattt
     4621 gttgccggga ttttcgccgg agttgcaagg tgtctatagc gaaaccgatg cggatggcat
     4681 gaaacgattg tacggttatg tgttgaagta agaataaaaa aacgggtcac cttctggcga
     4741 cccgtttttc tttgcgtaat tagtggctaa ccgtctgtgt gcctgtcggg acacgaacgt
     4801 gtttatattt gaacatcgcc atgaacgcga aggccagaac cacggaataa ccagcgaaaa
     4861 tcaaccatac ggtctgccag tcggtaatgc cgttttgggt gtacatctca acaactttac
     4921 cgctcacgat gccgccgagg atacagccga agccgttagt catcatcagg aacatccctt
     4981 gtgcactggc gcgaattgcc gggctaactt ctttttcgac aaacaccgaa ccagagatgt
     5041 tgaagaagtc gaatgcgcaa ccgtaaacga tcatcgacag taccagcagt acagtaccga
     5101 acggagtcgg gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc acaatactga
     5161 tcatcattac gttcttaata ccgtagcggc ttaagaagaa cgggatggtc agaatgaaca
     5221 gggtttcaga gatctgcgaa atcgacatga tgattgacgc atgctgcacg atgaagctgc
     5281 tggcaaacat cggatctttg tcgaagctgt gcaggaaggt attaccgaac atgttggtaa
     5341 tctgcagttc cgcgcccagc agcattgaga agatgaagaa gattgccata cgcttgtttt
     5401 taaacagcgc gaatgcatcg aggcccagca gggttgtcca gctctgattc gcttgctgtt
     5461 tagcaaccgg aatatgcggc agagtcaggg taaacagaac cagaatggcg gaaagtgctg
     5521 cgccaatata cagctgcatg tggcttaatt cgaagccaga caggctcacc acccacattg
     5581 ccatgataaa gccgatggtg ccccagatac ggattggcgg gaagtcagta acgatatcca
     5641 tcccggcatt ttgcaggcga tagtaagaga tggtgttgat taacccaagc gttggcatat
     5701 aagcaaacga gttaatcaat atcacaagga acatcgcttc cggcgtcgtg acctgtgccg
     5761 ccatgaacag cgtgatagcg ccaatggtgt ggcaaatggc atatacccat ttcgcactta
     5821 accatttgtc ggccacaatc cccagcagcg caggcataaa taccgcggcg atacccagtg
     5881 agctataaac tgcgccaata gaagcaccgt caaacttcag ggtaacaaac atataggagc
     5941 cgagggtcgt cagccaactt ccccacagac agaactgcag aaaagagagg attttcagct
     6001 gcagcttaag attcatgtta atttcctcac atcgtgatgc ggatgagtgt ttgaaaaagc
     6061 acatttgacc gtgcgagatg atgggattct atcaacttcg cggactggtt ttttgttacc
     6121 tgtggcaaat aattgcaaac attcccaatt tgcaaaacga aattgtgatg tggataacat
     6181 ttttgcccct gagcatcgtc aggggcggtt aatggaatta tcggcggcgg taggattttt
     6241 gcgcggtatt cactttataa agataacggc gagattccgc agagggatgg cgggtcgtca
     6301 gcgtctgata aacatcgccc ggcgtcatgg tgttaataat attggcagcc tgaatcttat
     6361 cattcgaaaa gactcgcagc acgctgcctg cgccgccgtt ataggcggtg atgacggcat
     6421 aacgccgcga tgttgggtta tcaattccgc cgagataaac attgttcagc atcgccagat
     6481 acgcggtgcc ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg ctcggcgtgc
     6541 cggatttccc ctgcgagcgg aacacatctt tcccggcagt attttgtacc acctgcatta
     6601 atcccagcgc atcggaacgg ctgaccgcat acgggttaaa ggaagattca gtctgcataa
     6661 ttgccagaat cagcgactca tcaacgccat atttccgtga cgcctggcgg accatgccga
     6721 gatatttgtg cgcacgttta tcaaggtggt tcggcaccat gttaatggtg acgctgtaga
     6781 tgatacgtag cccgttgctg cggcttttca gacggttttt cagcagataa tccgcgaagt
     6841 tgcttgcgcg accttcccag cgaatcggct gcccggtgtt gtccaccacc tgaccgtaaa
     6901 ggaaaggttc tttcgaaatt gtaatatcat caacgtcgga atagagatcg accgaactcg
     6961 gatcgtcacc catcagcaac gttttgataa ttgcccggcg caaatgcgcg gcaggttctg
     7021 tcccggcgat ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg ctgcgggtct
     7081 gatattgatc ggtgtatttc acgtagtcct taggaccggc gatcaccacc tctttgaagc
     7141 cccagatgtt ctcaatattg tgggcaaatt gccccatcag aatatcaaaa ccgttggtat
     7201 ctttgaccca ggcttcgtta taggtatcgc cttttttggt cgtcgaacag gagatgagca
     7261 acggcgcaat caaagccagc gcgagatatt ttttcatcat tccgggtgcg tgttgtgttt
     7321 gtttttatgc aagctgccgg aggcgtgctc cggcactgtt tttatttttt atcttccggc
     7381 gtatagccct cgatatgcac ctctttaccc tcgaacagga agttgaccat ctcctgctcc
     7441 agcagcttgc ggtgctcggc attcatcatg ttgagtttct tttcattaat cagcatggtt
     7501 tgtttgtgct gccactgcgc ccaggcttct ttggagatct cgttatagat gcgttttccc
     7561 agctcgccgg ggtacagctg aaaatcctga ccttctgctt cacgttgcag gaaagtacaa
     7621 aaaatcgttc tgctcataaa tcatcctctt tatcgactca cgcgctaaac cggcgcgcca
     7681 gtgcgtaact gctgtaataa acgctccacg ggagccgcca ggccaactga cggcggttgc
     7741 gctaagttat accagagcgc attgccttca tccatgcagc cggtgaatga cgacacggga
     7801 agccacatag gcacaatatc taagtggaaa tggctgaagg tatggcgaaa cgcggtcagt
     7861 tgcgtcaggt tatcggcagc aatctgccgt tgtgccagcc aatgccgcaa actttcttcg
     7921 tcggcaaact gcgggaaaca gtataaaccg ccccacaatc cgctcggcgg acgctgcgcc
     7981 agcaatactt catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc cggcagcgtc
     8041 tgtttcggtt ttttgcccgg ataaagcgac cagctattgt tggtggcggc aatacatccg
     8101 ttttgtagcg gacagagcga acatttcggc ttcgaacgcg tgcaaatcat cgcgcccaaa
     8161 tccatcatcg cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg ctcgctcaaa
     8221 ctccatagtt tattctcgac ctcttttttc ccaggccagc cgcttacagc atagcagcgc
     8281 gccagcacgc gtttgacgtt accgtcgaga atcggaaagt gcttacccag agaaagcgag
     8341 agaatcgcgc ctgcggtgga acgcccgacg cccggtaacg ccgcgacttc ttcaaaggtt
     8401 tccgggaatt taccaccgtg taaggtcgcc acttgttgtg ccgctttatg caggttgcgc
     8461 gcgcgggcgt aatagccaag cccggtccac aagtggagaa cttcgtcgag cggcgcattg
     8521 gcgagatcgg tcaccgtcgg gaagtgcgcc ataaagcgtt caaaataggg gataacggtc
     8581 gcaacctgag tttgttgcaa catcacttct gagagccata ctttgtaggg cgtcttgtca
     8641 atttgccagg gcagcgtttt ccgcccgtat ttatcgtacc agtccagaac ctgggctgaa
     8701 aattgcgacg cttgcatggt caccgacttc actgttgttg ggggcaagat tgcagcacag
     8761 cgacggcagg gtgtaaaccg gaactttccg cagccacggc cttatcatgc ttgcatccgg
     8821 caactaactt tggataatgc ccgttttcag aacactttca caagcgacta aatctttatg
     8881 aaaaacgacg tcatttcacc ggaatttgat gaaaacggcc gcccactgcg ccgtatccgt
     8941 agttttgtgc gccgccaggg gcgactgacc aaaggccagg aacatgcgct ggaaaactac
     9001 tggccggtga tgggcgttga gttcagtgaa gatatgctgg atttccccgc gctttttggc
     9061 cgtgaagcgc cggtgacgct tgagattggt tttggcatgg gggcgtcgct ggtggcaatg
     9121 gctaaagatc gccccgagca ggacttcctc ggcattgaag tgcattcacc gggcgttggt
     9181 gcgtgcctgg cttctgcgca tgaagaaggt ttaagcaacc tgcgcgtgat gtgtcacgat
     9241 gcggttgaag tgctgcataa aatgattcct gacaattcat tgcgcatggt gcagctcttt
     9301 ttccctgacc cgtggcacaa agcgcgccat aataaacgcc gtatcgttca ggtgccgttt
     9361 gccgaactgg taaaaagcaa actgcagctg gggggcgtat tccatatggc gaccgactgg
     9421 gaaccttatg cggaacatat gcttgaagtg atgtcttcta ttgacggtta taaaaacctg
     9481 tcagagagca atgattacgt accgcgtccg gcatcacgtc cggtgacgaa atttgaacaa
     9541 cgtggtcatc gtcttggtca cggagtatgg gacttaatgt tcgagagggt gaaataatgg
     9601 caaagaaccg tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc caggaattag
     9661 gattttcggt ggcatggcga ttcccggaag gtacatcgga agaacagatt gataaaatcg
     9721 ttgatgactt tattaacgag gttatcgaac cgaacaaact ggcctttgac ggcagcggtt
     9781 atctggcctg ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc gaagaacatc
     9841 aggcgattgt gcgtaagtgg ctggaagagt gcaaactgga tgaggtacgc accagcgaac
     9901 ttttcgacgt ttggtgggac taagaaagca tacgggcgat gacaaatgca aaactgcctg
     9961 atgcgctacg cttatcaggc ctacagagat gcacgatcga gtaggcggga taaggcgttt
    10021 acgccgcatc cggcatggaa aacgcgtact ttgttatcaa tctggggcca gcatatgctg
    10081 gcctgatttg ttcttgaggg aagactatga tgcgcaaaat gctgctggcg gcagcacttt
    10141 cagtgacggc aatgaccgct cacgccgact accagtgcag cgtcacgccg cgtgacgatg
    10201 tgattgtcag cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc
    10261 cagacggcaa cgtgatgtat aacggtaagc aatattccct gaatgccgcc cagcgcgagc
    10321 aggcgaagga ttatcaggct gaactacgta gcacgctgcc gtggattgat gaaggcgcga
    10381 aaagccgcgt cgagaaagcc cgtattgcgc tggataaaat tatcgttcag gagatgggcg
    10441 acagcagcaa aatgcgcagc cgtctgacca aacttgatgc gcagcttaaa gagcagatga
    10501 atcgcattat cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc attgatcagg
    10561 ttcgcgccga aggccagcaa ctagtgaatc aggcaatggg cggaatttta caggacagca
    10621 ttaatgaaat gggcgcgaaa gcggtgctga aaagcggcgg taacccatta cagaatgtgc
    10681 tgggaagctt gggcggtctg caatcctcaa tccaaaccga gtggaaaaag caggaaaaag
    10741 atttccagca gtttggcaaa gatgtttgta gccgcgttgt gactctggaa gatagccgca
    10801 aagccctggt cgggaattta aaataatcct ctattttaag acggcataat acttttttat
    10861 gccgtttaat tcttcgtttt gttacctgcc tctaactttg tagatctcca aaatatattc
    10921 acgttgtaaa ttgtttaacg tcaaatttcc catgcaaagc taagggataa tgcgtagcgt
    10981 ccacgtaact ggaggaatga aatggagttt ttcaaaaaga cggcacttgc cgcactggtt
    11041 atgggtttta gtggtgcagc attggcatta cccaatatca ccattttagc aaccggcggg
    11101 accattgccg gtggtggtga ctccgcaacc aaatctaact acacagcggg taaagttggc
    11161 gtagaaaatc tggttaatgc ggtgccgcaa ctaaaagaca ttgcgaacgt taaaggcgag
    11221 caggtagtga atatcggctc ccaggacatg aacgataatg tctggctgac actggcgaaa
    11281 aaaattaacg ccgactgcga taaaaccgac ggcttcgtca ttacccacgg taccgacacg
    11341 atggaagaaa ctgcttactt cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg
    11401 gtcggcgcaa tgcgcccgtc cacgtccatg agcgcagacg gtccattcaa cctgtataac
    11461 gcggtagtga ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt ggtgatgaac
    11521 gacaccgtac tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt agcgaccttc
    11581 aagtctgtta actacggtcc tctgggatac attcacaacg gtaagattga ttaccaacgt
    11641 accccggcac gtaagcacac cagcgatacg ccattcgatg tctctaagct gaatgagctg
    11701 ccgaaagtcg gcatcgttta taactacgct aacgcatccg atcttccggc taaagcactg
    11761 gtagatgcgg gctatgatgg catcgttagc gctggtgtgg gtaatggtaa cctgtataaa
    11821 tccgtgttcg acaccctggc aaccgccgcg aaaaacggca ctgcagtagt gcgttcttcc
    11881 cgcgtaccga cgggtgctac cactcaggat gctgaagtgg atgatgcgaa atacggcttc
    11941 gtcgcctctg gcacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcagct ggctctgacg
    12001 caaaccaaag atccgcagca gatccagcag attttcaatc agtactaatc acctcgtccc
    12061 ggcatcgtgc cggggctttt tcacttcaga cttacgtcca ttgccaattt tcattaccct
    12121 aatgataatc accggaataa attattccgc gcgagggttt tcgggtgaaa aagcaatgga
    12181 ttgttggcac ggcgctgctt atgttgatga ctggtaatgc ctgggcagat ggcgaaccgc
    12241 caactgaaaa tatcttaaaa gatcaattca aaaagcagta tcacggcatt ctcaagcttg
    12301 atgccatcag cttaaaaaat cttgatgcta agggtaatca ggccacttgg tcagcggaag
    12361 gcgatgtctc ttccagtgac gatctctata cctgggtcgg tcagttggca gattacgaac
    12421 tgctcgaaca gacctggacg aaagataaac cggtaaaatt ctcggcgatg ttaaccagta
    12481 aaggaacgcc cgcgtctggc tggtcggtga acttttactc ttttcaggcg gcagccagcg
    12541 atcgtgggcg ggtggtcgac gatatcaaaa cgaataataa atatctgatc gtgaatagcg
    12601 aagatttcaa ttatcgcttt agtcagcttg aatctgcgtt gaatacccag aagaactcga
    12661 ttcctgcgct ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg gcgcagaaag
    12721 cagcggatgc atattgggga aaagatgcaa acggtaagca aatgacccgc gaagaagcct
    12781 ttaaaaaaat tcaccaacag cgtgatgagt ttaataaaca gaacgatagc gaggcgttcg
    12841 cggttaaata tgacaaagag gtttatcaac cggcgattgc ggcatgtcat aaacagagtg
    12901 aagagtgtta tgaagtgccg attcagcaga aacgagattt cgatatcaac gaacaacggc
    12961 gacagacttt tctgcaatca caaaaactca gccgtaaatt gcaggatgac tgggtaacgc
    13021 tcgaaaaggg caatatccgt taacgatgaa agtttcagaa ataaacagta aaaaagtcgc
    13081 gattttgatg aaaattgacg atatcaacca agctaatgaa cgctagaaaa aagataccga
    13141 acaactgcgg cgcaatgggg tgattaagta actcatgtta agtgcctttg ccggataata
    13201 taaaaagtta tccggcaaat tattttttac attagcccta gtagctgtgg aatgaacaag
    13261 gatatttgcg gaatgtaagt gatcatcatc aacgctacta gcaatgcagc ataaaatagc
    13321 agcaatggtt tgattaaatg ttgaatcttc acacccgaga tagaacaacc aacaaatagc
    13381 gcactaccca ccggcggtgt taacagccca atacaaaggt tggccaccat cataataccg
    13441 aaatgtactg ggtccattcc cagctcttgt gcaatcggca agaagatcgg tgtaaagatt
    13501 agtacagccg gtgtcatgtc cataaagatg ccgacaatta ataagacgat attaatcagc
    13561 agcaatatga tcagtgggtt atcagaaatc cccatcaatg cgtcgctgat catataagga
    13621 atatcagcat tagtcattgc ccaggacatg cccacggaaa aaccgatcaa cagtaataca
    13681 atcgaagtgg taacaaccga ctccagaatc agtttgggta aatggcgcca ttttacttcc
    13741 cggtaaatca gcaccgagag aataaatgtg tagacaacgg caattgctga cgcttcagtt
    13801 gcggtaaaaa tgccgcccag aatgccgccc agaatgccgc ccatcacaat gaacaccaga
    13861 agcaaacttg gtaaggcatc cagaaacgct ttacaggcct gagcaaaggt tgctctttct
    13921 gaaagcgggt agccacggcg tttagcaata atgccgcaga ctatcattac cgcaagaccc
    13981 attaagatcc ctggcaaata tcccgccata aacagcgatg ctacagaaac gccgccagct
    14041 gtcagggaaa acacaattaa cacgtttgag ggcggtatca gtaatccagt aatacaagat
    14101 gagacgttta cagcggtaga aaatgccgga tcataacctt ctttggtctg aatgggattc
    14161 agtgtgccac caacggcagc agctgccgca acggctgaac cagaaatgga accaaacatc
    14221 atattcgcca ggacgttcac atgaccaagc gaaccaggaa cgcggccaac cattacctgg
    14281 gcgaggttaa tcaaacgtat cgcaataccg ccgctgttca tcagtgttcc ggcaaagatg
    14341 aaaaagggga ttgccaacag ggcaaagtta tccagtccat tagccagtcg ttgcgaaaca
    14401 gtgattgccg cgatatccca gggaaacatc aaaagcattg aagctacagt ggcaataccg
    14461 attgagaacg aaatgggcac accaataaag accagcacaa aaaaacagcc aaacagtgta
    14521 atcgcgatat aactatccat tggtaatgct gccaacttac tgatttagtg tatgatggtg
    14581 attttaaggt gcttgcgtgg cttccatttc catcagatgt c
//
LOCUS       DAECNO010000098        14567 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.098, whole genome shotgun sequence.
ACCESSION   DAECNO010000098 DAECNO010000000
VERSION     DAECNO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.098"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..581
                     /locus_tag="KKO08_003163"
     CDS             <1..581
                     /locus_tag="KKO08_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBD6319315.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="KKO08_003164"
     CDS             complement(635..1357)
                     /locus_tag="KKO08_003164"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBD6319316.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="KKO08_003165"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="KKO08_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KKO08_003166"
     CDS             complement(2557..4173)
                     /gene="bglH"
                     /locus_tag="KKO08_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBD6319317.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KKO08_003167"
     CDS             complement(4259..5653)
                     /gene="bglB"
                     /locus_tag="KKO08_003167"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBD6319318.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KKO08_003168"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="KKO08_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBD6319319.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KKO08_003169"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="KKO08_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBD6319320.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="KKO08_003170"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="KKO08_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBD6319321.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="KKO08_003171"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="KKO08_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBD6319322.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="KKO08_003172"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="KKO08_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBD6319323.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="KKO08_003173"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="KKO08_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBD6319324.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="KKO08_003174"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="KKO08_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBD6319325.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14567)
                     /locus_tag="KKO08_003175"
     CDS             complement(13620..>14567)
                     /locus_tag="KKO08_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6319326.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3758 a         3718 c         3317 g         3774 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata
    14341 gcaatcccaa catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca
    14401 gcataagtta atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg
    14461 ttaccagtat cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta
    14521 aatgtcgttc caaccttatt ttcgttcgcg gtaataagat atcccga
//
LOCUS       DAECNO010000099        13963 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.099, whole genome shotgun sequence.
ACCESSION   DAECNO010000099 DAECNO010000000
VERSION     DAECNO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.099"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KKO08_003176"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KKO08_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD6319327.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KKO08_003177"
     CDS             2219..2767
                     /locus_tag="KKO08_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBD6319328.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KKO08_003178"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KKO08_003178"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBD6319329.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KKO08_003179"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="KKO08_003179"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBD6319330.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KKO08_003180"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="KKO08_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBD6319331.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KKO08_003181"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="KKO08_003181"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBD6319332.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KKO08_003182"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="KKO08_003182"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBD6319333.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="KKO08_003183"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="KKO08_003183"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBD6319334.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KKO08_003184"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="KKO08_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBD6319335.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KKO08_003185"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="KKO08_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBD6319336.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3561 c         3525 g         3325 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgtc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DAECNO010000100        13957 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.100, whole genome shotgun sequence.
ACCESSION   DAECNO010000100 DAECNO010000000
VERSION     DAECNO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.100"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            297..1550
                     /gene="kch"
                     /locus_tag="KKO08_003186"
     CDS             297..1550
                     /gene="kch"
                     /locus_tag="KKO08_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBD6319337.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1605..1778)
                     /locus_tag="KKO08_003187"
     CDS             complement(1605..1778)
                     /locus_tag="KKO08_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBD6319338.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1921..3381
                     /gene="cls"
                     /locus_tag="KKO08_003188"
     CDS             1921..3381
                     /gene="cls"
                     /locus_tag="KKO08_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBD6319339.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3416..3745
                     /locus_tag="KKO08_003189"
     CDS             3416..3745
                     /locus_tag="KKO08_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBD6319340.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3798..4802)
                     /gene="oppF"
                     /locus_tag="KKO08_003190"
     CDS             complement(3798..4802)
                     /gene="oppF"
                     /locus_tag="KKO08_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBD6319341.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4799..5812)
                     /gene="oppD"
                     /locus_tag="KKO08_003191"
     CDS             complement(4799..5812)
                     /gene="oppD"
                     /locus_tag="KKO08_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBD6319342.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5824..6732)
                     /gene="oppC"
                     /locus_tag="KKO08_003192"
     CDS             complement(5824..6732)
                     /gene="oppC"
                     /locus_tag="KKO08_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBD6319343.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6747..7667)
                     /gene="oppB"
                     /locus_tag="KKO08_003193"
     CDS             complement(6747..7667)
                     /gene="oppB"
                     /locus_tag="KKO08_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBD6319344.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7753..9384)
                     /gene="oppA"
                     /locus_tag="KKO08_003194"
     CDS             complement(7753..9384)
                     /gene="oppA"
                     /locus_tag="KKO08_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBD6319345.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10122..10769)
                     /gene="ychE"
                     /locus_tag="KKO08_003195"
     CDS             complement(10122..10769)
                     /gene="ychE"
                     /locus_tag="KKO08_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBD6319346.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11246..13921
                     /gene="adhE"
                     /locus_tag="KKO08_003196"
                     /gene_synonym="adhC"
     CDS             11246..13921
                     /gene="adhE"
                     /locus_tag="KKO08_003196"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBD6319347.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3661 a         3454 c         3245 g         3597 t
ORIGIN      
        1 gagatccaat atttaagggc acggtttttg caaccgtgtt cttgtttggc ctttccttat
       61 agccggtcag tgcattcact accacaagca gataataact ttatctgtat ttctttatat
      121 gatgtgagca tgttcaggaa aaaagccctt tcctgacgca gagttgctca ctcaaaacct
      181 ttgattactg cggataaagt catctactat tgtttaacac attcacgcgc tgcatgtatg
      241 ttcagaaaat gtgcacgcta agataattaa acaaaattgt ggttacagag gtatcagtga
      301 gtcactgggc tacattcaaa caaacagcaa caaatttatg ggtgacccta cggcacgaca
      361 ttctcgcgct ggccgtcttt ttaaatggat tgcttatttt taaaacaatc tatggtatgt
      421 cggtcaattt gcttgatatt ttccatatca aagcattttc agagttggat ctctccttgc
      481 tggcaaacgc cccactattt atgctcggcg tctttcttgt cctgaactcc attggcttac
      541 tgttccgggc aaagctcgca tgggcaatca gtatcatttt gttgttgata gcgctaattt
      601 acaccctgca tttttatccc tggctgaaat ttagtattgg attttgcatt tttacgctgg
      661 tgtttttgct gatactgcgc aaagacttct cccacagtag cgccgcagcc gggacaattt
      721 ttgcatttat tagtttcacg acattactgt tttactcaac ctacggtgcg ctttatttaa
      781 gcgaaggttt taatccgcga atagaaagtt tgatgaccgc gttctatttt tcgatagaaa
      841 ccatgtcaac cgtcggctat ggagatattg tccctgtttc tgaatcagca cgattgttca
      901 ctatttcggt cattatttcc ggcattaccg tttttgccac atctatgacc tcaatttttg
      961 gtccgcttat ccgcggggga ttcaacaaac ttttaaaagg aaacaatcat acaatgcatc
     1021 gtaaagatca ttttattgtt tgcggacatt cgattctcgc catcaatacg atcctgcaac
     1081 tgaatcaacg cggacaaaac gtaacggtta tcagcaactt gcctgaagat gatatcaagc
     1141 aacttgagca acgcttaggc gataacgctg atgttatccc cggtgacagt aatgacagtt
     1201 cagtattaaa gaaagcggga atcgatcgtt gccgggccat tctggcgctg agtgataacg
     1261 atgcagataa cgcgtttgtt gtactctcgg caaaagatat gagcagtgat gtcaaaacag
     1321 ttctcgccgt cagtgatagc aaaaacctga ataagattaa gatggtacat ccggatatca
     1381 ttctctcacc gcaactgttt ggcagcgaaa ttctggcgcg agttttaaat ggtgaagaga
     1441 ttaataatga tatgctcgtt tcaatgttgt tgaactcagg tcatggtatt ttcagcgata
     1501 acgatgaaca agaaacgaaa gctgacagta aagaatcggc gcaaaaatag agtttaaaga
     1561 cggaagagta aacttattgg gcaccgcaat ggtgcccagt acatttagat attgtagatc
     1621 gcaaacaata acgagttacg ctgtttgttt agaatgcact tcctgatgct gtggatacgc
     1681 atatttcggc gcgattgccc ctcaagccaa cgcgatttac gtcggctagc ctgacgctgc
     1741 atgcgccagc gccccacttc cgttctactc cgcttcatgt taactactct atgcaataac
     1801 agaatggtca ttataccccc gcccccgacg ctgaccagtg cttttcccgc gtttttattt
     1861 gccagatgaa tccatatgcg taaactcata acaatgcgct ttcaaaagga tttctaatct
     1921 atgacaaccg tttatacgtt ggtgagttgg ttggccattc tgggatactg gttgctcatt
     1981 gcaggcgtaa ctttacgcat tctaatgaaa cgacgcgcag ttccctccgc gatggcctgg
     2041 ctgttgatta tttacattct gccgttagtc ggaattattg cctatcttgc cgttggcgag
     2101 ctccatttag gcaaacgccg cgctgagcgc gccagagcga tgtggccttc caccgcaaaa
     2161 tggcttaacg accttaaagc ctgtaagcat atcttcgccg aagaaaatag cagtgtcgct
     2221 gcgccattat tcaagctttg cgagcgtcgt caggggatcg ctggggtcaa agggaatcag
     2281 ctacagctga tgaccgagtc agatgatgtg atgcaggcgt taatccgcga catccagctc
     2341 gcgcgccata atattgagat ggtgttttat atctggcagc ccggcggcat ggcggaccag
     2401 gtggctgaat cattaatggc ggctgcacgg cgtggtattc attgccgatt gatgctcgac
     2461 tccgccggga gtgtggcttt tttccgcagc ccgtggcccg agctaatgcg taatgccggt
     2521 attgaagtgg tcgaagcctt aaaggtcaat ctgatgcgtg tgtttttacg ccgtatggac
     2581 ctgcgccaac atcgcaagat gatcatgatc gataattaca tcgcgtacac cggcagcatg
     2641 aatatggtcg atcctcgcta cttcaaacaa gatgcgggcg tagggcaatg gattgatctg
     2701 atggcgcgta tggaaggccc catcgccacc gcgatgggga ttatttattc ctgcgactgg
     2761 gagattgaaa ccggaaaacg tattctgccg ccaccaccag atgtcaatat tatgccgttt
     2821 gaacaggcca gcggtcacac cattcacaca attgcttctg gccccggctt tccggaagat
     2881 ctcattcacc aggcattatt gactgcggct tattcggcgc atgaatattt gatcatgacc
     2941 acgccctact ttgtgccaag cgatgattta cttcatgcga tttgcacggc ggcgcagcgc
     3001 ggggtggatg tcagtattat ccttccgcga aaaaatgact cgatgctggt cggctgggcc
     3061 agtcgcgcat tctttacgga actgcttgct gctggggtta aaatttatca gtttgaaggc
     3121 gggttactgc ataccaagag cgtgctggtc gatggcgaac taagtctggt tggcaccgtt
     3181 aaccttgata tgcgtagtct gtggctgaat ttcgagatta ccttggcaat cgacgataaa
     3241 ggttttggtg cagaccttgc cgccgttcag gacgattata tttcgcgttc acgtctgctc
     3301 gatgcccgtt tatggctaaa acgtccatta tggcaacgtg tcgccgagcg actgttttac
     3361 ttcttcagcc cgttgctgta aaacgtcgcc aacagaggtt aaacaggtag tgactatgga
     3421 tatggatcta aacaatcgcc tgactgaaga tgaaacgctt gagcaggcct acgatatttt
     3481 tctcgaactg gctgccgaca atctcgatcc agctgatgtt ctgctgttca atcttcagtt
     3541 tgaagagcgc ggcggcgcgg agttattcga tccggcggaa gactggcagg aacatgttga
     3601 tttcgatttg aacccggact tttttgccga agtggtgatt ggtctggcgg acagtgaaga
     3661 tggtgaaatc aacgatgttt tcgcccgcat tctgttatgc cgcgaaaaag atcacaaact
     3721 ttgccatatc atctggcggg aataaataat cgcttttagc gtgaaaaagg gctgacaact
     3781 gtcagccctt attgttttta aagcggatcg actttcaggc aagaaacggc gtgtctgaaa
     3841 ctcccctcca gaacaggacg tgttttggcg cactccggac cggcaatcgg gcaacgggta
     3901 cggaaaacac aaccggaagg cgggttgatc ggcgacggta attccccttc cagtaactgg
     3961 atggttttgt tcttctccag atccggatca ggtatgggga ctgccgacat caatgccctg
     4021 gtgtaaggat gtagtggatt gtggtagacc tcatcatagg tccccagttc tactgcatgg
     4081 ccgagataca tcaccaacac acgatcggaa atgtgtttta ccacggccaa gtcatgagca
     4141 ataaaaatta atgacaatcc catctcacgt tgcagctgct ggagcaggtt aaccacctgc
     4201 gcctgaattg acacgtccag cgccgaaacc ggctcatcgc agataatcag cttcggttca
     4261 agaataagag cacgagcaat cccgatacgc tggcactgcc caccggagaa ctcatgcgga
     4321 tagcggttaa tcaggttagg caataacccg actttcagca tcatcgcctt cacgcgctcg
     4381 cgaacttcct ggcgtgacat tttcggatga taagtacgca gtggttcagc gatgatctcg
     4441 ccgatggtca tacgcgggtt tagcgatgcc aacggatcct ggaaaatcat ctgaatatca
     4501 ctgcgaacgg cacgccattc atcgggcttc atgcccagca actctttacc taaccaggca
     4561 acatgaccgt cggtcgcctt gaccaaaccg atgatggcgc gagcaaaggt ggacttaccg
     4621 catcccgatt cccctaccac acctaatgtt tccccttcat acaggcgaag agtgacacca
     4681 tcgacggctt tgagcgtttt cggcggttgc cagaaccact gtttgccatc tttgatttca
     4741 aagtgcactt taagatcggc gatttcaagg aggacttttc ttccttcagt tacagcattc
     4801 ataacagttc ctccaccggt ttaaagcaag cacgcagacg gccaggcgta aactcttcca
     4861 gcggcggtgc gctactacaa atttccatcg catgtggaca acgtggctgg aacgggcaac
     4921 ctttcggtaa tcgcagcaag tttggcggat tacccgggat ggtcaacatt gtttcacctt
     4981 ccgcatcaag acgcggcacc gcgttgagca aaccgataga ataaggatga acgggttgat
     5041 aaaagacatc gcgagcattg ccatattcca tcgtgcgccc ggcgtacatt accagcactt
     5101 tgtcacagat ccccgccacc acgccaagat cgtgggtgat cataatgatg gcagtattaa
     5161 attcccgctt cagttcattc aatagcgtca tgatctgcgc ctgtacggtg acgtccagcg
     5221 ctgtagtggg ttcatccgca atcagcaact taggtcgaca tagcaatgcc atcgcaatca
     5281 tgactcgctg acgcatgccg ccagaaaatt cgtgcgggta cattttcatg cgtttacgtg
     5341 cttccggcat ttttaccgca tcgagcatcc gcaccgactc ttcaaacgcc tcagctttgc
     5401 tcatgttctt atgcagcatc agcacttcca tcaactgctc accgacgcgc atatagggat
     5461 tcaacgaagt cattgggtcc tgaaaaatca ttgagatttg ttcagcgcgc agtttattga
     5521 gttcacgctc tggcaaattg aggatttcac gcccattgaa ggttgccgat ccgccaatgc
     5581 gtccgttggc agccaacagg cccatcaacg caaatgcagt ttgcgattta cccgaaccag
     5641 actcacctac aatgcccagc gtttctccgg cacgtaggga aaaatttaaa tcattgaccg
     5701 ccgtgacgtc gccgtccggg gtactaaagg tgacacgcaa atctttcacg ttcagcagtg
     5761 cgtcagcctg ttgttgtgcg agcggcacag ttgcagtttc aattacgctc atggctgcac
     5821 tccttaacga tctttcgggt cgagggcatc acgcaagcca tcgccgataa agttgaaaca
     5881 aaacagtgtc accacaagga atcccgctgg gaacaacagt aaccatggag agacttccat
     5941 cgagttcgcg ccatcactca gcaatgcccc ccagctgctt aacggctctt gcgtacccaa
     6001 ccccaggaag ctaaggaaag attcaaagag gatcatgctg ggcaccagta gcgatgcgta
     6061 gaccaccacc acaccgagta cgttcggcac aatgtggcga ataacaatgc ccggcgtcga
     6121 tacaccgcca acttgtgccg cctcaataaa ctctttgcgc ttcagactca gggtttgccc
     6181 acgcacaata cgagccatat ccagccagga aaccatgcca atcgccacga aaatcagcag
     6241 gatgttttgg ccgaaaaagg tcaccagcaa aatgacgaag aacatgaatg ggaaggagtt
     6301 gaggatttcc agcagacgca tcattaccga atccacttta ccgcccagat aaccggaaag
     6361 cgaaccataa agtgtcccca cgaccactgc caccagtgcc gcagcaacac cgaccatgag
     6421 tgagatacgc ccgccaatcg caacgcgcac aagcaggtcg cgaccggatg agtcagtacc
     6481 aaagtagtga ccggactcca tatccggggc gctggacatc atcgcccagt cagtatcgtc
     6541 ataggcaaac tgggaaagca tcggtgccag gattacaaat aacgcgatca gcaccagcac
     6601 tatcagactg gcaaccgccg cacggttatg cataaaacgt cgacgtgcgt cctgccacaa
     6661 gctgcgccct tcgacctcca gcttttcact gaaattttcc agcgtctcgc tgtttttctt
     6721 acttaacatc atcgcgagct ccagtatcag taacggattt tcgggtcgat aaccgcatat
     6781 agcacatcga caatggcatt aaacaaaatg gttaacgcac caaccaggat ggtcaggctt
     6841 aacactaagg aatagtcacg gttcaatgcg ccattaacga acagttgccc aatccccggc
     6901 aaaccataaa tggtttcgat gaccatagaa ccggtaataa tgccgacaaa tgcggggccc
     6961 atataggaga gcacgggtaa cagagcaggt tttaatgcgt ggcgtaaaat gatccgccgc
     7021 ataggtaacc ctttcgcccg ggcagtacga ataaagttgg agtgtaatac ttcaatcata
     7081 gagccacggg taatacgcgc aatactggcg atataggcga gtgacaacgc caccatcggc
     7141 aatatcatga atttaagcgc cccaccattc cagccaccgc ccggcagcca atgcaaaatg
     7201 atcgcaaata tcatgactaa taatggcgca accacaaaac tggggataac aaccccggtc
     7261 attgccagcc ccatcacggt atagtcccat ttggtgtttt gttttaatgc ggcaataacg
     7321 ccagcactaa cacccagtat taccgcaagg aaaaatgcgg cggctcccag tttggcagaa
     7381 acgggaaaac tggatgccac caggtcattg accgaataat ctttatattt aaacgatgga
     7441 ccgaaatcgc catgcgccag ttgtttcagg tagctgaaat actgtgtcat gatcggatca
     7501 ttaagatgat atttcgcttc gatattggcc atcacttctg gcggtaaagt acgttcgccg
     7561 gtaaaagggc ttcccggcgc gaggcgcatc ataaagaacg aaatagtaat aagaataaat
     7621 agcgtcggaa tcgcttccag acaacgacgt agaataaatt ttaacattgc cctaccttct
     7681 ggcctgtgcc ttctgtaatg cgataaaaat cagacaccgt ggagcaggac actcctgccc
     7741 cacgtattgc cattagtgct tcacaatgta catattccgg gtataggtat tatccagcgg
     7801 atctttgccg gtatagccac caacccacgg tttcaccaga cgcgcattca cgtagtaata
     7861 aacaggaaca atggccgaat ccttatccag ctgttgttct gctttagtgt acagagctgt
     7921 gcgctgcgcc tcgtcagtca ctttcagcgt ttccgccata atgctgtcaa aggccgggct
     7981 cttataatgc gcggtattca tcgagctgtt cgacagcatg gtgttcagga aggaagttgg
     8041 ttcgttgtag tctgcacacc agcctgcacg ggccacatca aaagtgccct ggtgacgggt
     8101 gtcgaggaac gttttccact cctggttaac cagtttgacg tttaccccaa tgtttttctt
     8161 ccacaatgaa gaggcagcaa tcgccagctt tttatgcaga tcggaggtgt tataccgcag
     8221 gttgatggtc aacggtttgt ctgcggtata acccgcttca gccagcagtt ttttcgcttc
     8281 ttcgttacgt ttttcctggc tccagccaaa ccattctggc tgagtcaatt ttgcgccatc
     8341 agtatacggt ggagtataac cataggcggg catgtcgccc tgcgctttca ctttattaac
     8401 aatgatatcg cggtccatac ctagtttcag tgcggtacgc acacgcacat cgttgaatgg
     8461 cggtttctgg ttgttaattt cgtaatagta agtgcacagg tatggatcaa cgtgaacttc
     8521 gtccgggatc tcttttttca gcttctggaa caattcgatc ggcatgttgt tatacgtcat
     8581 gtcgatttca ccactacggt agcggttgac atcggtaact tcagaagcaa taggcaaata
     8641 ggttacctgg ttaataacgg ttttcgcgtt gttccagtag gtcgggctgc gttcaagaac
     8701 gattcgttcg tttacgaccc aatcttttaa ggtataggca ccgttggtga cgatattacc
     8761 aggctgggtc catttttcgc cgaatttttc gatagcggct tttggcaccg gtgaagttga
     8821 tgggtgaaca agtaatttat agaagtacgg aacgggttca cttaaggtga cttctaatgt
     8881 gtgatcatca atagctttca cgccgagatc ggtaatcggt tttttccctt caagaatttc
     8941 atcaataccg gcgatatgcc catattgcag ataactggca tacggagaag cagtgttcgg
     9001 atcaacagaa cgttgccagc tatacacaaa gtcttgtgct gtgactggcg tgccatcaga
     9061 ccatttcgca tctttacgca aatggaaggt ccagactttc gcgtctttat tatcccagga
     9121 ttcagcgacg ccaggtgctg gatgaccgtc aagatcgctg accagtaagc cttcaaacag
     9181 gtctcggctg atattagact ccggaacacc ttcaattttg tgcggatcta atgactgaac
     9241 ttctgaacca ttgttacgta ccagtgtttg tttttccgcc agtgtgacgc ctgcgggtac
     9301 atcagctgcc agcgcgacat tccctgccat tagcgcagcc agaacgccag ctgctactaa
     9361 acttctcttg gtgatgttgg tcattgtttt ttggactccc tcattataat tactggtatt
     9421 aaccagcatg tgtaatcccg gattgggacc ctgcacaagc tcggagactt tctgctgtca
     9481 ggttacttta tcgttacgta ttactatcac cgactttatt tattctggtg attcagacga
     9541 tcaccttatg tcgattcgta ctgtgtctcc cctgtcgaga cacattattc tttcgcgacg
     9601 ctctttaatt gttaattatt ctcattacca ataaagaatc gtctggcggt tgcccggaaa
     9661 gtaccaaatg cggttcttac tcgccaatac attttgcaaa attgttaagc gattctcttt
     9721 tattgaccgt atcagaatct atagcttgca tccatgcctt aacaaacatc tttcaattca
     9781 atcaaataga gattggctaa gcatgtctat cttttctgat tacagttcca gtagtgaaat
     9841 gcacaataat ttaacaattg attattattt agcactttcc tctaccaaag gcagtggaat
     9901 tactaatatc atttcaatta ttcttcagca agcccaggat tatgatgtgg cgaaaataac
     9961 atgatggtga ggagaaacag ttaaggaaat ccttataact aactgtaatt tgagaagaat
    10021 atttttacct attccttttg cttttgataa ctgcgcggga acaaagtaaa aataatgacg
    10081 aagtaaacgg agcatggcag ctccgtttca tttgaaagga attaattaag caggccgggg
    10141 aaaatcccct taataccagt aacgataaat tcaatcccca atgccatcag caatagcccc
    10201 ataatacgcg taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag ccacggtgcc
    10261 atgcggaaca atccccagca acataaagcg aacaatgcaa tagccacaaa gaaaccaaac
    10321 agatagctaa tgctgtgata acgcgtaccc cagacgatgg tagaactgat cgcccctggc
    10381 cccgccatca acggcaacgc caatggcacc acaccaatac tttcacgtac cgcggtttct
    10441 gatttttctt gcttgttctg tttatcctcg ccaagcttgc cgctgatcat cgacatcgct
    10501 atcgtcacca ccaggatgcc cccggcgata cggaacgaat caattgatat accaaaaagt
    10561 tgtagaatcg tgtcgccgag aaaaagcgag atccacaaga taatggccac agacaggttg
    10621 gctgtaaggt tagttttgtt tcgcgctgcc gctgtctgat aactggtcat gctgataaag
    10681 acgggaataa tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa tttgaagtaa
    10741 acgggaaaat caaaaagggt ctgaatcacg gttcgctccg aagcaaaagc cggataatgt
    10801 tagccataaa taaggttgaa aagacgcgct gacaatacgc cttttgacag catttttcac
    10861 ctcctaacta cttaaaattg ctatcattcg ttattgttat ctagttgtgc aaaacatgct
    10921 aatgtagcca ccaaatcata ctacaattta ttaactgtta gctataatgg cgaaaagcga
    10981 tgctgaaagg tgtcagcttt gcaaaaattt gatttggatc acgtaatcag tacccagaag
    11041 tgagtaatct tgcttacgcc acctggaagt gacgcattag agataataac tctaatgttt
    11101 aaactctttt agtaaatcac agtgagtgtg agcgcgagta agcttttgat tttcatcggt
    11161 taagcaaatc atcaccgcac tgactatact ctcgtattcg agcagatgat ttactaaaaa
    11221 agtttaacat tatcaggaga gcattatggc tgttactaat gtcgctgaac ttaacgcact
    11281 cgtagagcgt gtaaaaaaag cccagcgtga atatgccagt ttcactcaag agcaagtaga
    11341 caaaatcttc cgcgccgccg ctctggctgc tgcagatgct cgaatcccac tcgcgaaaat
    11401 ggccgttgcc gaatccggca tgggtatcgt cgaagataaa gtgatcaaaa accactttgc
    11461 ttctgaatat atctacaacg cctataaaga tgaaaaaacc tgtggtgttc tgtctgaaga
    11521 cgacactttt ggtaccatca ctatcgctga accaatcggt attatttgcg gtatcgttcc
    11581 gaccactaac ccgacttcaa ctgctatctt caaatcgctg atcagcctga agacccgtaa
    11641 cgccattatc ttctccccgc acccgcgtgc aaaagatgca accaacaaag cggctgatat
    11701 cgttctgcag gctgctatcg ctgccggtgc tccgaaagat ctgatcggct ggatcgatca
    11761 accttctgtt gaactgtcta acgcactgat gcaccaccca gacatcaacc tgatcctcgc
    11821 gactggtggt ccgggcatgg ttaaagccgc atacagctcc ggtaaaccag ctatcggtgt
    11881 aggcgcgggt aacactccag ttgttattga tgaaactgct gatatcaaac gtgcagttgc
    11941 atctgtactg atgtccaaaa ccttcgacaa cggcgtaatc tgtgcttctg aacagtctgt
    12001 tgttgttgtt gactctgttt atgacgcagt acgtgaacgt ttcgcaaccc acggcggcta
    12061 tctgttgcag ggtaaagagc tgaaagctgt tcaggatgtt atcctgaaaa atggtgcgct
    12121 gaacgcggct atcgttggtc agccagccta taaaattgct gaactggcag gcttctctgt
    12181 accagaaaac accaagattc tgatcggtga agtgaccgtt gttgatgaaa gcgaaccgtt
    12241 cgcacatgaa aaactgtccc cgactctggc aatgtaccgc gctaaagatt tcgaagacgc
    12301 ggtagaaaaa gcagagaaac tggttgctat gggcggtatc ggtcatacct cttgcctgta
    12361 cactgaccag gataaccaac cggctcgcgt ttcttacttc ggtcagaaaa tgaaaacagc
    12421 tcgtatcctg attaacaccc cggcttctca gggtggtatc ggtgacctgt ataacttcaa
    12481 actcgcacct tccctgactc tgggttgtgg ttcctggggt ggtaactcca tctctgaaaa
    12541 cgttggtccg aaacacctga tcaacaagaa aaccgttgct aagcgagctg aaaacatgtt
    12601 gtggcacaaa cttccgaaat ctatctactt ccgccgtggc tccctgccaa tcgcgctgga
    12661 tgaagtgatt actgatggcc acaaacgtgc gctcatcgtg actgaccgct tcctgttcaa
    12721 caatggttat gctgatcaga tcacttccgt actgaaagca gcaggcgttg aaactgaagt
    12781 cttcttcgaa gtagaagcag acccgaccct gagcatcgtt cgtaaaggtg cagaactggc
    12841 aaactccttc aaaccagacg tgattatcgc gctgggtggt ggttccccga tggacgccgc
    12901 gaagatcatg tgggttatgt acgaacatcc ggaaactcac ttcgaagagc tggcgctgcg
    12961 ctttatggat atccgtaaac gtatctacaa gttcccgaaa atgggcgtga aagcgaaaat
    13021 gatcgctgtc accaccactt ctggtacagg ttctgaagtc actccgtttg cggttgtaac
    13081 tgacgacgct actggtcaga aatacccgct ggcggactat gcgctgaccc cggatatggc
    13141 gattgtcgac gccaacctgg ttatggacat gccgaagtcc ctgtgtgctt tcggtggtct
    13201 ggacgcagta actcacgcca tggaagctta tgtttctgta ctggcatctg agttctctga
    13261 tggtcaggct ctgcaggcac tgaaactgct gaaagaatat ctgccagcgt cctaccacga
    13321 agggtctaaa aatccggtag cgcgtgaacg tgttcacagt gcagcaacta tcgcgggtat
    13381 cgcatttgcg aacgccttcc tgggtgtatg tcactcaatg gcgcacaaac tgggttccca
    13441 gttccatatt ccgcacggtc tggcaaacgc cctgctgatt tgtaacgtta ttcgctacaa
    13501 tgcgaacgac aacccgacca agcagactgc attcagccag tatgaccgtc cgcaggctcg
    13561 ccgtcgttat gctgaaattg ccgaccacct gggtctgagc gcaccgggcg accgtactgc
    13621 tgctaagatc gagaaactgc tggcatggct ggaaacgctg aaagctgaac tgggtattcc
    13681 gaaatctatc cgtgaagctg gcgttcagga agcagacttc ctggcgaacg tggataaact
    13741 gtctgaagat gcattcgatg accagtgcac cggcgctaac ccgcgttacc cgctgatctc
    13801 cgagctgaaa cagattctgc tggataccta ctacggtcgt gattatgtag aaggtgaaac
    13861 tgcagcgaaa aaagaagccg ctccggctaa agctgagaaa aaagcgaaaa aatccgctta
    13921 atcagtagcg ctgtctggca atataaacgg ccccttc
//
LOCUS       DAECNO010000101        13857 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.101, whole genome shotgun sequence.
ACCESSION   DAECNO010000101 DAECNO010000000
VERSION     DAECNO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.101"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(1..819)
                     /gene="wcaD"
                     /locus_tag="KKO08_003197"
                     /pseudo
     CDS             complement(1..819)
                     /gene="wcaD"
                     /locus_tag="KKO08_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
     gene            complement(794..2011)
                     /gene="wcaC"
                     /locus_tag="KKO08_003198"
     CDS             complement(794..2011)
                     /gene="wcaC"
                     /locus_tag="KKO08_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBD6319348.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2008..2496)
                     /gene="wcaB"
                     /locus_tag="KKO08_003199"
     CDS             complement(2008..2496)
                     /gene="wcaB"
                     /locus_tag="KKO08_003199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBD6319349.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2499..3338)
                     /gene="wcaA"
                     /locus_tag="KKO08_003200"
     CDS             complement(2499..3338)
                     /gene="wcaA"
                     /locus_tag="KKO08_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBD6319350.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3431..5593)
                     /gene="wzc"
                     /locus_tag="KKO08_003201"
     CDS             complement(3431..5593)
                     /gene="wzc"
                     /locus_tag="KKO08_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBD6319351.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5596..6039)
                     /gene="wzb"
                     /locus_tag="KKO08_003202"
     CDS             complement(5596..6039)
                     /gene="wzb"
                     /locus_tag="KKO08_003202"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBD6319352.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6045..7184)
                     /gene="wza"
                     /locus_tag="KKO08_003203"
     CDS             complement(6045..7184)
                     /gene="wza"
                     /locus_tag="KKO08_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBD6319353.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7493..7642
                     /locus_tag="KKO08_003204"
     CDS             7493..7642
                     /locus_tag="KKO08_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319354.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7843..9426
                     /locus_tag="KKO08_003205"
     CDS             7843..9426
                     /locus_tag="KKO08_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBD6319355.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9878..11731)
                     /gene="asmA"
                     /locus_tag="KKO08_003206"
     CDS             complement(9878..11731)
                     /gene="asmA"
                     /locus_tag="KKO08_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBD6319356.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRRNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11753..12334)
                     /gene="dcd"
                     /locus_tag="KKO08_003207"
     CDS             complement(11753..12334)
                     /gene="dcd"
                     /locus_tag="KKO08_003207"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBD6319357.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12426..13067)
                     /gene="udk"
                     /locus_tag="KKO08_003208"
     CDS             complement(12426..13067)
                     /gene="udk"
                     /locus_tag="KKO08_003208"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBD6319358.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13385..13804
                     /locus_tag="KKO08_003209"
                     /pseudo
     CDS             13385..13804
                     /locus_tag="KKO08_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
BASE COUNT         3208 a         3654 c         3509 g         3486 t
ORIGIN      
        1 gtacctaaat cgcccagacg ggtggaaata taaggaaacg cgattaccac accaacccgg
       61 aatacagcca gagaaatcaa tgctaacggt aattttttct taatcgcctc tttaattcag
      121 atattgaaat gcccactcca gcaaataaaa caggataaag gtcataaccc ctgaaaacga
      181 tccggataat attatccctg cgagaatcag ggcatctgtt ttaggcgttt tgataccaaa
      241 ctgtttgatg ctgagccaaa ttgagattaa tgccagagcg aaaaatgccg gttcgaaata
      301 aagtgctgtc gtgcgcttgc cgccgaattt aatgaaattc agcacatagc tgttgctgta
      361 aatcagatat ttcgaaatcg actccataat actgctgcca ccggtgagga taatttgcgc
      421 catctccacc gccgccagcg ccaccaccag tccgaccacc agataaaaga aacgtaatat
      481 cttacgatgg ttgtgtggcg aaattgtttt aaaacgaata ctccacacca tgccaataat
      541 gatcacaata tagacaaaca gcatagttga agtgacgtat ttaccggcat ccaacgactg
      601 accaaacagg tagttaaacg ccgtgagtcc cgcgccaatc cctaaggcaa tcatcaattt
      661 tttaatgctg atgcgttcta aaaacagcag taacaagaca ggtaaaaaag tgacgatggt
      721 gatggggaaa ctttcgccaa gctgggcgat tttgacgtta accagcaggt agatcagtgg
      781 cagcagcagg tagctacaga ttctgataga agttgacata ctcctccagc atctgttgtc
      841 cactgtaggc ggcgcgactg cgttggctga actcagccag cgtggtacca aatatcgcct
      901 gcgcgatttc cggtttgctt aactgtacca gttgcagcac ctcttcttca ctgacggttt
      961 taccaccgga tttttgcagc acttcccgcg ccgcatcgct atgggtggca atcaccggca
     1021 cgccaatcga tagcgcctca cacaaaatca gcgggtagtt atcgacacga gaactgaaca
     1081 ccagcgcatc catctgattg agcgcgctca tcaacttgcg cttgtcagtt tcaaagccgt
     1141 gattaaccac gttgccagcg gtgaacggcg agaacttacc aaaggtatgc agttcgattt
     1201 tgtcgcccag cgccatcatc tcacgcacca gttgctggtt agttttgccg tcgtaacgca
     1261 ggtcatgcgc caccactgcg attttcggct tgccctgagt ttcgcgcacc ggaggcagat
     1321 ctgccagaat cgcttcggtt gccatatcaa tgccattatt gataatccgg caacgccctg
     1381 gcccgtacag actattgaaa gcgtcagcca catgctggct gggggaaata aactgacagc
     1441 ccagcgccag tatctcgcgg aataactggc gtttgcccgc caccagctgg tgcgcgcgat
     1501 caatcttcac cggcggatag ttatttaagg tcgggcattt ctggcagccc gttttccagc
     1561 cttcgcaacc gtcggtaaag gcgcagcgtc cggttacact ccagtggtcg tgcagcgtcc
     1621 agaccagggt gacatccggt ttatggtttt tcactttttc gcaaaagcgc accacgctgt
     1681 taagattcaa ccagtagctg tgcagcacat gaaaatgcag gaccaccgga cccggtgtgc
     1741 gagtaatggt gcgatataac tcattgaaat tgccgaacag atcgcgatta aacagacgaa
     1801 acaacgcaat gttcgccatc gcggtcatcc gcggcgtatg tttgatgacc tgcggatagt
     1861 tctgatggct gacgctctct ttgccgcctt tgccgtagcc gtagacaaaa tgtgacgcca
     1921 gcccctgttg cagcgcacgc tggtggagat ctaacgccac acctgctgcc ccgccttccg
     1981 ccagtcgcac attaaattgc agaatattca tttaatcacc ttcactcgcg ctttttctcc
     2041 caccaccagc gcgttgtccg ggacagagtc gagcaccacg ctgcccgcac ccacggtgac
     2101 gttgttacca agcgtgatat caccaagaat aatgacgttg gcaccgagtt cgacgccgtt
     2161 gccaatgtgt ggacatgcca tgttatcggc accccgattg ccgatagtga cgccgtggcg
     2221 aatggtgaaa tcatcccccg ccaccacgtt tttattgatc actacggcgt aaccgtgatg
     2281 gatagtaaag cggcggccaa tggtcgcggc ggcctggatt tcataaccga aaaagcattc
     2341 ggtgataatg cgatacagca ccagcagcgg ggccgcccac agattgttga ggacgttctt
     2401 tttgcgccac accgaacaaa aatgagcgac gcgataggca agtaccatgc agcacgggcg
     2461 taaactccag ctgttggcgc gcagatcttc cagcatcttt agcgcccccg aataccatca
     2521 gccagacgtt tgccgttacg caccgacagc agcgtcaaca aggtgcgcca ggtcatacgt
     2581 ttattgcgga tctggtagag ggtaaacagc tgatattttt tactggcgcg gtcgaatttg
     2641 tctttgtgct tgcggtaaaa gtggaagtat ccggagaatt ttttcggtga cgaggtgatc
     2701 tgcatttcgc cgtgattgat atgcaggatc tgcgtcgcct cttccacttt ccacggttcg
     2761 ccgtactcca ccaccatccg caggaatatg tcgtaatcct gtgctgcttt cagttcggta
     2821 tcgaacaaac actctttgaa acgccatgcc caggtaaaga cctgattgcc aatgatatta
     2881 cgtttataga acaggcggcg tgagtacggc gatttgggat acagtggtaa gctcgccggt
     2941 tgggaataaa cttctccctg gcaaacgtag tcgttagcgt acaaaaaggc gtgtgtgacc
     3001 agttgctgtt tatgggcgag gaagacgctc aggcggttgg gcgtccattc atcatcatca
     3061 tcgatcccgg tgatgtattc cccttgcgcc agcataatcg cctggttacg caccgcgcac
     3121 gccccgctgt taatgtcgtt gtggatgtaa gtgatacgcg gatcgttgag ggcagtgacg
     3181 tactgttgca gttgttccca ggaggtggag caatcatcca cgatgatcat ctcccagttg
     3241 ctgtagtcct ggcgcaggac cgattttatc gcccgaatcg ccagttgttg gcggttccag
     3301 gttggcatat agattgagat cagcggattg tctttcatag gttgttctcc cggcttatga
     3361 gctggtttgc ttgccggatg cggcgtgagc gccttatccg gcctacgggg cggtgcgaat
     3421 gcaggcgtcg ttatttcgca tccgacttat attcgtattc gtaatagcca taatcctgat
     3481 acgcgctggc gcggcggaag atggagttca gaatcacccc tttcaccgga ataccgtttt
     3541 gctcaaagcg gctcagactg gtttccactt ctttcaatgt gttgaccgca taacgcgcca
     3601 ccattaacgt ggttccgaca tgacgaccaa caattgccgc atcggtcact gccagaatcg
     3661 gcggcgtatc aatcaacacc aggtcatagt ttttactcgc ccagttcacc agttcggcaa
     3721 agcgttcgct cattaacagt tcagaaggat ttggcggcac ctgaccgcgc gggatcaggt
     3781 caaatttggc aatagaggtc ggtttagcag cggtagtaat atcgccctga ccaatcagaa
     3841 tctccgacag cccattgaca ttattggtgc ccaacagctc gtgggtgtag cctttgcgca
     3901 tatcgcagtc gatcaacaac acgcgtttat tggtctggct gatcaccgcc gccaggttgg
     3961 cgcagacaaa ggttttaccg attgacgggc taaccccggt catcatcaac acattgttct
     4021 gcgcctgcat catcgcgaag tgcagactgg tacgcagact acggatggct tcaatcgcca
     4081 gatcggttgg attccccact gccagtagct ggctctgttt atagcgttta acccctttga
     4141 tggttttgac gctatcgcgc gctttctgcc actccgacag cgggatgctg gcatagacgc
     4201 tgattccgtg ttcttccagc acttgcgggc tttcgatgcc gcgattaaac aacgagcgca
     4261 gcagcacacc cacgatagag agcatcaggc caaggataat cgcgccgagg ataatcagcc
     4321 ctttcttcgg ttttagcacg ccaggctgag tgattgccgg gtcaataatg cgcacatcgc
     4381 cgacggtgct ggcttcggtg attttcagct cctgctcttt attcagcagt tgcatataga
     4441 cctgctgacc agactcgaca tcgcgggtca gacggacaat ttcctgctgg tttttcggca
     4501 tcgccgttac gcgaccgtta agtttggctt tctcttcttc cagcgcctga cgtttctcca
     4561 gcagcgtgcg gtacgccggg tgaactttgg tgtacagctt ggagatttcc gcctctttaa
     4621 aggtcagttc gttcaactgc gcgtcgatgt tcaccatcga atcgagcacc gctttcgctt
     4681 caagcggcag atcaacagaa tctttatcct gacggaaggc attcagtttg ttttcggcaa
     4741 catcgaggcg gctacgtact tccggtaact gttgcgcgag gaaagcgagg cttttcgacg
     4801 cttccgccga tttgcgctca atattttgtt cctgatagtt acgagcgatg ctgttaagaa
     4861 tgtcgcggat ctgttcgcga tcttcaccgg tataagtcag gctcagtacg cctgcgtctt
     4921 tgccgttctc cgttaccgtc aggctgtttt gcagttggtt gatcatcccc aacgtggagt
     4981 atttggtgac ggtaaactca ctgcccgggc gggcgtgaat ggcttcaacc atcagcgtga
     5041 cgccttcttt tttcagcatc tggcccgctt gcccacgggc gctaaagccg ccatcactgc
     5101 tcagggtgta gtttttgtca tccagcacat taagcgtaaa cacctgatcc gccatctctt
     5161 tcgggcggtt aaaggtagtc actttcaccg tctcgttctg acgccccatc aggcgatccc
     5221 agcccgcccc gaaaatcggg aacgtgtttt tgctcaccgc aatatcgagg tcgagatcgt
     5281 ccaccgtttt accaagcacc agacgcgaac gaatcaactg gatctcggcg tccgatgcag
     5341 gcggtttgtt ggctaatgcc gagccgatat cctgcaccag cgaattgccg ctgttttgct
     5401 cgatttgtac cagtgcgtcg gcactataaa tcggcgtggc gaagaaagtg taaaccacgg
     5461 cacagagggc aaatacgacg gtgatgccaa tcacccacca gcgcgcttca atgacggtgc
     5521 cgaccaggcg accaatatcg atttcatcac tgcccgttac cggagcggca tgttgtttta
     5581 ctttttctgt cattcttata cctgctctgc gttcaatgcc tgcgcccact ggcgggcaga
     5641 ccgttcaagt aatgtgtaca ccgctgcaaa cgtttcccgg cttttgcgat acggatcggg
     5701 gatttcacat tcgttatccc agtgaccaaa cagcatcact ttgccgcgca tctccggtgc
     5761 catctcgcat aagcgttcga tatggcgctt atccatggtc aaaatcaggt catagttgcg
     5821 acacaggcgg cggctgattt gtcgtgcaca gtggccttcc agagacagtt gatgttctgc
     5881 ggcgacgctg atagcggtag gatcagcgcc cttaccgacc agcgcgccga gtccagcgga
     5941 ctccactttc agttccgggt gataacgttg cagtaagcgt tccgccatcg gggaacggca
     6001 aatattgccg acacagacaa ctaagatgtt gttaaacatg acgattacca gttatgaatg
     6061 tcgctggctg tatccgtcat gtaacggaca ccgctaatag ttggcagcaa ctgattgatc
     6121 agacggttcc agcgggaaac cggggcggtg gtgacataca ccacgtcata aggttgcagg
     6181 cggaattctg tcgccatcac cagcgacgtc gcatcggaca tatccagctg gtagatattg
     6241 gcaatcttgc cgttacgccc gccctcgcct ttcagcggac gaatgacaaa gatgccgctg
     6301 gcgttggagg tggtcatgtc gataccttcg gcattggcca gagcttcagt cagggtcatg
     6361 ccgctaaagt ccattttcag ggtgctctgt ttcttcactt cacccatcac aaatactttc
     6421 agatcatcat tacgcggcac gtagagaata tcgccgggat aaagcagtcg gttctgattg
     6481 aggtcgccgt tttgcatcag cgcctgtaaa gaaatgcgtt cttcacgacc attgtgtgtt
     6541 agcaccacgt tgcgccagtc agcggtgtcg gtcaggccac ctgcggcgtt gatggcgtcg
     6601 agaatggtca gtggtacgtt ggtgatcgcc tgttgaccgg atttattcac ctgacctgag
     6661 atataggcct tttgtgagcg gaaggcggcg atattaacgt ccacctgcgg gtcagcgatg
     6721 tacgtcgcta agcgcccggt aatatcactg cggatttcag cgagcgtttt cccgactacg
     6781 tgaaccttgc cgatatacgg gtaaaacata gtgccgtcag gctgtaccca gttgccggtg
     6841 tcgctggagc tgcggtactg accagctggc gtggtgagtt ccgggtgatc ccagacggtg
     6901 acattaagaa cgtcccccgg cccgacgcga tactggtaat tcgcgatctc gctttccagc
     6961 gtcatattgg ggcgcgctac attcgggcgt gggcgtaatt ggtcaatcag gcgcggggtc
     7021 agcggataaa cattcaccat tttgtcgaga tcgaaatcag cgtcctgctg tttgatgacg
     7081 tctttgccca tcgtcgacat attgctgccc ggaagtactg tgcaaccgct tatcaaggtt
     7141 actgacacca ataatggcat caatttcatt ttggatttca tcattgttta tttatcactt
     7201 tggcagagta attatcctgt gcactattaa tagcaatgtc gccatgcaca tttaccttgc
     7261 agttaattga ataaaaattt aactggcatc agtcctaaaa aaattgattt catccgcagg
     7321 ctattgacag aataattcag actggtcttt caggcatcca gacacgctac cgcccctggc
     7381 tttttagcta ccaatacact gattgagctt aatttttcaa accctctctg catacaatcg
     7441 ttgatgagaa agggttattg cggaaattaa cttccgaata taaggtgaca ttatggtaat
     7501 tgaatattgg ctcgccaata atgcaggagg aagtgttaca gctaacggaa tagcaggcaa
     7561 ggtaacaatt cggcaattgg ctatttttaa gaattatatt aagtatcatt caatatggtt
     7621 tttaggagtt tctttaggtt gacaatattt aatattgcgt ctccacatgc gatatttctt
     7681 aaataatgtt ttattattac cgcttttaat tcagggataa tgtgaggtta ttaccctcaa
     7741 ataatatgag ataaatagtg ctgcaacatt gcatttttgc cccgatttat ccatgatcga
     7801 attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg gattgccgat
     7861 ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct cggcattgat
     7921 aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca
     7981 cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctagc gtcaatctcc
     8041 tggctggtca ccctgactca accgctgttc agcttccgct cgtttacctt tagcgcccgc
     8101 gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat ggagctgaac
     8161 gaacggctgg aagggaaaga cagcaataat cccacacaac gcaaaggtgc gaagttctgg
     8221 ggcgtggtga cgcaaattgt ggtactggac gccattttct cactcgactc agtgattacc
     8281 gccgtcggga tggtcgacca tttactggtc atgatggccg ccgtggttat cgctatcagc
     8341 ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc gaccatcgtt
     8401 attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga aggtttcggc
     8461 ttcgtcattc cgaaaggcta cctgtacgct gccattggtt tctcggtgat gatcgaggca
     8521 ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaatca gacgctacgc
     8581 cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg
     8641 gacgccaaaa ccgcgtccat gctggtggat catggaaacc agcagatctt taatccgcag
     8701 gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtaccgtaag cagcattatg
     8761 acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagaaga aatccgccag
     8821 ttgctggagc gaaatcagca tacgcggctg gtggttaccg acggtgatga cgcagaagat
     8881 ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt cactgcgcgg cgaaccgctc
     8941 aacctgcggg tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc gttgttacca
     9001 gccctggagc agttccgtaa tgcccgcacg cactttgctt ttgtggtgga tgagtttggc
     9061 tcggtggaag ggattgtgac attaagtgac gtcactgaaa ccattgccgg taacttaccg
     9121 aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga cggttcctgg
     9181 acggcgaatg gtcatatgcc gctggaggat ctggtgcaat atgtgccgct gccgctggat
     9241 gaaaaacgtg aatatcacac cattgccggg ctgttgatgg agtatttgca gcgtattcca
     9301 aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca ggtggaaagc
     9361 catcgcgtgc agaaggtgca gattataccg ctgcgtaagg atggcgagat ggagtacgag
     9421 gtgtgatggt atcggccatt ttacccaatg ctgcgtaacg ccttataact tcagcaatga
     9481 agcgttgtct gtggtaaacg gacatcacag cgggggtgtg ggtccggctg taaatcgccg
     9541 aatcgtttcc tgtaggctgg ggcagcccac atggatgtgg gctgagggcg cgttttacag
     9601 ggatgttacc tcgcgcccgt acccgatagc cgaaaggaat aagatgaggg aaccgcgcca
     9661 gcggcgattt accgccggga gcccgggttg ccagggtggt ggcgattgag ccaccctggc
     9721 acgttcaccg gcttgagcgt tccatagtag cgaggaacat caagtgaacg gaacagcctt
     9781 cgatcttaca tattcagaat ataatagtgc cgccgcgaac gcctgatgcg acgctttacg
     9841 cgtcttatca ggcctacagg acaggtcacc tggtaaccta catcttctcc agcaacttct
     9901 tcacatcttt gccattgcgg gaatctttat tccgctcggc ccagtcattc aggcgacgtt
     9961 tcgcttcgtc ctgtagatgt ttgcgcagta actgatccac ttgcaggctg taattgagtt
    10021 gctgccagtt gccataaacc cgcagcggta ctggcgtttc tttgaggaaa tcaatcagtt
    10081 tgctttcccc gttccagcca cccacgaccc gaatatcaaa ctgggtgtcg caggtttgat
    10141 ctgccagatt caacatgcct tcccctgtca gcgccagcac aggcgattga ccttgcatgt
    10201 cgtttaacgt cacgacgcca tccttcaacg tcaaatcggt ggtaaagcgg tcaagccgcg
    10261 tcacgttatc gaagttttca gcggccttca catcaccgcc attacgctct accgcttgct
    10321 gaatcatctg ctggaagttc atcccttcca tacgtgtgtc ggtcatctca acatgcgcct
    10381 gtccttgcca gttgcggcgg aatgcgtcgg catctatgtc agcaccggag aaatcaccag
    10441 ccagtgacat ttttccggtc aacgaaatcg gatagttaaa cgccttcaga atggtaccaa
    10501 tctcaacgtt ctccagccgt ggctggaagt ttatccgcgg atttattgat gtcgcgtcca
    10561 gcgtgcccgg cagtgaaacc tttccaccgt taagcttgcc ctgcagttga gtaatttcca
    10621 gcaaacccga cttgttggtc atttgcgtgg caacatctgt aaaattcatt ccgcgccagc
    10681 gcacgttact ggcctgcaac aaaatatcag ctgtaaagcc ttgcagtccc tgataggccg
    10741 gttcatcaat acgcgaagaa atgaccgggc gcggcaacgt tgattggctc tgcccctgct
    10801 gcgcggcacc gttttcacca ttcgcggttt cattaagcgg gatgaggttg tcaagattca
    10861 gttgcgggaa ttgcagtctc agctgccatt ccggtttctc tgtcagcgtg acctgtgctt
    10921 gtccgctcag cgtactgtca ttggcggtca aactaatttg gttaaatgaa aggcgtttat
    10981 gtgactcctg ccactgggac tggaaactcc cctgcccctg aataccttgt tttggtaaat
    11041 cggcaccctg caactgccag ttaatttgtt caatagccgc tgttaaatca tgcggataat
    11101 cagaagcatc taccgtaccg ttaagggata tcgtgagatc gcgctgatcg cgattaaccc
    11161 gcccggagaa ctcaaatgag ccacgatgtt ggggatcttg ttccatttgc aggcggatat
    11221 tgcggattgt cacctgctcg tcatcttcat gctggaacac cagaacgctg tccgccacct
    11281 taagactgga tatatcaaac gaccatccgc gatcgtctga cagatccggc aaggtattgt
    11341 cgcgcggtgc aaccggagcg tcttcactgc gcaccgcttc cgtctgcggc gtcagttgga
    11401 tcactgcccc ttttagcatc acctgcttaa cgctcagttg atgactcagt agtggtaaaa
    11461 gcgccacgtc cagacgcatg ttgtcggcgc gaaccagtgg ctggcttgcg ccctgggcgg
    11521 tgagagacat tcgcccggag aggatactaa gctgcggcca gacgtgccaa cgcagtggcc
    11581 cgtcgagctg caattgataa ccgctacgtg cagcaacttg cttgaccata tagtcgcgga
    11641 aatcattcgg attcaccagc aacactaacg cagataaccc ggccaccagc acgaccaaga
    11701 gtatcatcag cgtcgtcaga aatcgtctca tggtatcctc aatgggcctg aattagtctt
    11761 tatcgattcg gctggctacc gcgccctgct ggttgcgata tttcgcatct tcacggcggt
    11821 tgtaaggtcg cgccgccggg ccggaaagcg gctcaaagct cagcgcacca attaacatgc
    11881 ccggacgcag cgccagcggc agcttaccgg agttgtagaa ctccagcaca atgcaaccag
    11941 accagcccgg atcgatacgg tgcgcggtga cgtgcaccat cagccccaga cgcgccaaag
    12001 aggaacgccc gtccagccag cccaccagat cggctggcag cgtcaccgac tccagtgtca
    12061 ccgccagcgc cagctctcct gggtgaagat agaacgcctc gccctcgtcg agaacgatct
    12121 catcgctcat cacgcggtca agcgcggcgc tcacttcatc tttgggaccg ctcagatcga
    12181 taaacgctgc cgtgtgacca cggaaggtac gaaatttatt gcccaggcgt acatccaccg
    12241 tcgcgccgtt aatacgctcc actggcggac gtgggttgat cgacaaacgg ccttcatcaa
    12301 gccaggcttc aatatctcgg tcacacagac gcatggcatt ttctcctttc gcacatcact
    12361 cccttaacgc caattacgtc aagggcatac caacttatca ctgaacggta cacaatttat
    12421 caagcttatt caaagaactg acttattttc gctttcaata tatcgatcgc gatgcggttt
    12481 ttcccgccgc gcggcacgat aatgtccgca tattgtttag aaggctcaat gaattgcagg
    12541 aacatcgggc gcacggtttt ttgatattgc gccatcacgg aatccattga acgcccacgc
    12601 tcgttaacgt cacgcttgat gcggcgcatc aggcagatat ccagcggggt atcaacgaaa
    12661 atggagaagt taagttcgtc acgcaaacgc gcatccgtca gcaacaaaat gccttcgaga
    12721 atgatgacct tcttcggctc aaccgtcacc gtttctttca tacgcgtatg ttcaacatag
    12781 ctgtaaaccg gcaggtcaat tgccgagccg cgtttcaacg cttgtaaatg ctcaagcaac
    12841 aggctgtgat ccatcgcgct gggatggtcg tagttggtct taacgcgttc ttccatcgac
    12901 agatggcttt gatctttgta atagcagtct tcgggaatta cgccgatgtg ttcatcaccg
    12961 acttgctcac gtaattcacg ataaagggta ctggcaataa gactcttgcc ggaagccgat
    13021 gcgccagcga taccgataat gacgcactga tgagactgat cagtcatata tttagcgacc
    13081 tgattaacct ggatgttagg aagggggcga cgaagcgcca aacgcggcaa ttatagggat
    13141 ttcatccgtc tgataccagt cgaatagcgt tgccgcgcgc tcagagttaa ttgttgacaa
    13201 agaattcccg gtggcaaatt acgttgatca gttttatgta aggtaaaaaa tgttatacgc
    13261 agttgcgcaa attatccgcc tttacgtcac tttatgagca attcgcatat aaaatgtaaa
    13321 acttttgtac tagcataaac acagaaacga atactggcga cctggccttg cggataaagc
    13381 ggtaataagc aaacaatcac agcatgtatt aattgccctg ccccacccgc tgcttcacct
    13441 ggtcagttta ggtttagtct cgtttatctt tacccttgtc tcgcttgagc tttcgcagtt
    13501 tggcacccaa ctcgccccac tgtggttccc gacgtccatc atgatggtgg cgttttatcg
    13561 ccatgccggg cgcatgtggc cgggaattgc gctgagctgt tcgctgggaa atatcgccgc
    13621 atccatcctg cttttttcca ccagctcgct gaacatgacc tggacgacca tcaatattgt
    13681 tgaagccgtg gtcggggcag tgctgctacg taaattgctg ccgtggtata accccttgca
    13741 aaatctggct gactggctgc gtctggcatt cggcagcgcc attgttccgc ctctgttggg
    13801 gggtgcccct attaagttgt caagcatgtt atgacccctg cggggtataa aaagtcc
//
LOCUS       DAECNO010000102        13532 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.102, whole genome shotgun sequence.
ACCESSION   DAECNO010000102 DAECNO010000000
VERSION     DAECNO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.102"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..890)
                     /locus_tag="KKO08_003210"
     CDS             complement(<1..890)
                     /locus_tag="KKO08_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6319359.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="KKO08_003211"
     CDS             1131..1931
                     /locus_tag="KKO08_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBD6319360.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="KKO08_003212"
     CDS             2009..2779
                     /locus_tag="KKO08_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD6319361.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KKO08_003213"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="KKO08_003213"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBD6319362.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KKO08_003214"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="KKO08_003214"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBD6319363.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="KKO08_003215"
     CDS             5046..5768
                     /locus_tag="KKO08_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD6319364.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KKO08_003216"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="KKO08_003216"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBD6319365.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="KKO08_003217"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="KKO08_003217"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBD6319366.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="KKO08_003218"
     tRNA            7161..7237
                     /locus_tag="KKO08_003218"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="KKO08_003219"
     CDS             complement(7368..8414)
                     /locus_tag="KKO08_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBD6319367.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KKO08_003220"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="KKO08_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="KKO08_003221"
     CDS             complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="KKO08_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBD6319368.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="KKO08_003222"
     CDS             complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="KKO08_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBD6319369.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11797..12627)
                     /locus_tag="KKO08_003223"
                     /pseudo
     CDS             complement(11797..12627)
                     /locus_tag="KKO08_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="KKO08_003224"
                     /pseudo
     CDS             complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="KKO08_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3103 a         3522 c         3609 g         3298 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccatccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca
    10981 gatcgccttc ttccgtatcg ggatgcaact gcgccacgct cattgagggc gtgggtgtga
    11041 cgtaattggg gctgaccact tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa
    11101 tttttagctg cgtgcgggcg gtcaggaagc tgaaggcctc gaccattcgc tctacatacg
    11161 gatcggcaat atccgttccc tgcatgccca gccgggcggc gactttggga tgacgggcgg
    11221 caaattcggc tcccgtttcc cgcagatagc tcagttcgcg gttgtagtat tccagtagcc
    11281 gtggatccat cggtctctct ctgaattaaa aaaacgtaat gcgactctgc tccatatcca
    11341 gcgcgctacg tacccggaat gcagtcggat acggctgcgt gaggatctcg ccgcgaattt
    11401 caaactgtaa tgtgttatgg ccactctggc ggcgctcatc ctgaagtggg atgatttgca
    11461 gagtggacgc tttcagccgg ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga
    11521 tatcgtccca cttgtgctca tacagaaagc tgcccgccag tggcggcagg ccgtagttaa
    11581 gtacggacgc tgccgcgtac ggataacgtg cagcatcaat atcatcttca tggctgacgg
    11641 tgttgagcag aaatgacaaa tcccggcgga tgatctcctt aatctgaacc ggactgacgc
    11701 tgataccgtg gtcgcgtttc tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg
    11761 gcagcaggtg attgcctgga cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt
    11821 gttaaaggta caggtggaca aatccagcag gctgatatcg ccgtgaccgg tgagccacac
    11881 cctctgaccc agcgcctgta ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg
    11941 gcacagacgc agggagtcgg cagccttttc agagccgctg tagcggggaa acagctaggc
    12001 actgtgtgta tcgccattgt tcagggtgac gttgacgggt ttccagatca aatcgatcag
    12061 atgcgcaggc cgcggcgatt tcagcgaaca gatttgtgaa aacggcagcc agatatatgc
    12121 tccgcccgcg atcagttcga gtaccggacc ggtacgggag tcgctgtcgc taatccagtc
    12181 aaatgcgccg ccattccact gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg
    12241 gtgagcatca gcttcctgcg cttcaccacg ggcgttacag gcgagggcgg tcagcagatc
    12301 ctccatccag gcgggcggcg ggagaataac accgggccgc tgttcgccct aaaagcaggc
    12361 gtggcggtat atctcacagc ggatcagctc gccaaatacc tgcgcttcgc gagtataatt
    12421 tgcgtccatc cgggcgcata gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac
    12481 gcacaataac tgaaacaggc tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg
    12541 ttgctctgcc atctgaatgc cctccaccac ggaataatcc cgcagcagcg ccgacaaggt
    12601 aatcggtagc tcgttattgt ttgtcatgat atatctctcc atgttgtgcc gggtttatcc
    12661 ggtaatggct gtcaatgcca atgatgcgat gctctttccg tgtcagatct ggcagtacgg
    12721 tgttatgcgc tgtttccggg gcgtattccg gggaaagtaa atgcaggata tcagggggag
    12781 tcttttcgac aggccagata gtttcatctt cgccagtggc atccagtaca ttgagaatag
    12841 cgtcaatgct taacccacca gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt
    12901 cacgcaatga atccagatcc tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat
    12961 ggcgattaag atcttgttcg ggagataaca acgcctcgcg gtattcctga taaagtcggg
    13021 tcagggcatt tcctttttcc gcaccttctc cggtggagga ggctccatga acaggaatga
    13081 tatcgaatgg attttgtgat gtcagatgct gctgcctgaa ccaatccaga tccagatatt
    13141 cggttattgc ttccgacgtg atggcagtct gcgatatcgt ttttactgtg ctcaggttcg
    13201 gagatgatgg attgccatgt acaaggcact atgaagataa cccccattct atcaggtcgc
    13261 cttcattcag acgcatacgg tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat
    13321 attcggtaga ctgattgata atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa
    13381 tctttacatc atcattcacc ttaaaaacag gaatccaggg agaggtaacg gtgaagataa
    13441 tttgtgttcc ttcagtcgcg taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt
    13501 ggagtcccca cgtaatattt ggtttcattg tg
//
LOCUS       DAECNO010000103        13049 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.103, whole genome shotgun sequence.
ACCESSION   DAECNO010000103 DAECNO010000000
VERSION     DAECNO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.103"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(992..1368)
                     /gene="rnpB"
                     /locus_tag="KKO08_003225"
     ncRNA           complement(992..1368)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KKO08_003225"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1401..2546)
                     /gene="garK"
                     /locus_tag="KKO08_003226"
                     /pseudo
     CDS             complement(1401..2546)
                     /gene="garK"
                     /locus_tag="KKO08_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(2643..3533)
                     /gene="garR"
                     /locus_tag="KKO08_003227"
     CDS             complement(2643..3533)
                     /gene="garR"
                     /locus_tag="KKO08_003227"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBD6319370.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3563..4333)
                     /gene="garL"
                     /locus_tag="KKO08_003228"
                     /pseudo
     CDS             complement(3563..4333)
                     /gene="garL"
                     /locus_tag="KKO08_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(4349..5683)
                     /gene="garP"
                     /locus_tag="KKO08_003229"
     CDS             complement(4349..5683)
                     /gene="garP"
                     /locus_tag="KKO08_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBD6319371.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6058..7629
                     /gene="garD"
                     /locus_tag="KKO08_003230"
     CDS             6058..7629
                     /gene="garD"
                     /locus_tag="KKO08_003230"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBD6319372.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(7681..8505)
                     /gene="agaR"
                     /locus_tag="KKO08_003231"
     CDS             complement(7681..8505)
                     /gene="agaR"
                     /locus_tag="KKO08_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBD6319373.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            8754..10034
                     /gene="kbaZ"
                     /locus_tag="KKO08_003232"
     CDS             8754..10034
                     /gene="kbaZ"
                     /locus_tag="KKO08_003232"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBD6319374.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10057..10530
                     /gene="agaV"
                     /locus_tag="KKO08_003233"
     CDS             10057..10530
                     /gene="agaV"
                     /locus_tag="KKO08_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBD6319375.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10541..11320
                     /locus_tag="KKO08_003234"
     CDS             10541..11320
                     /locus_tag="KKO08_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBD6319376.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11310..12119
                     /locus_tag="KKO08_003235"
     CDS             11310..12119
                     /locus_tag="KKO08_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBD6319377.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            12206..12640
                     /locus_tag="KKO08_003236"
     CDS             12206..12640
                     /locus_tag="KKO08_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBD6319378.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12637..>13049
                     /locus_tag="KKO08_003237"
     CDS             12637..>13049
                     /locus_tag="KKO08_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBD6319379.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         3212 a         3460 c         3257 g         3120 t
ORIGIN      
        1 cagataaatt agaaaatgaa ttaacaaaag aaatatataa aagctcatcg aaaatcaaca
       61 attgcttgaa cagaattaaa ttaccagaga tgaaagaatt ctcatcaaaa aaaatatatg
      121 attacatcga tacaatcatt gaagattatg aaaatatcaa agaaaatgct tatctggtcg
      181 tcccccaaat taattatact atggatttaa acatagaaga ctctagctca gaagagttaa
      241 taccctcgag aaagacgaaa attctccgga caatggcttt gaggtcgggg aatataacac
      301 atatgaagca tataactcag agaatcaata ttttaccaga gaggactata cgtataatta
      361 cgacctttta aatgcaatat agacttaata acttcttaca aagtctatta ttaataccgt
      421 agatattcat cttaccctgt gcaccaaaag caaaaactta tcgacgcggg tttgaaactg
      481 atggcaaccg caaaaatgcc tgatgcgcta cgcttatcag gcctacgcca tctctgcaat
      541 atattgaatt tgcgtgcttt tgtaggcagg ataaggcgtt cacgcagcat ccggcatgaa
      601 taaagcgcac tcaacaatct gaaacccgcc ggaacggttt attacgtaca tcaggtgaaa
      661 ctgaccgata agccgctttc ttttgggtat agtgtcgtgg acagtcattc atctttctgc
      721 ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt atatcaggtg aaactgaccg
      781 ataagccgct ttcttttggg tatagcgtcg tggacagtca ttcatctttc tgcccctcca
      841 aaagcaaaaa cccgccgaag cgggttttta cgtacatcag gtgaaactga ccgataagcc
      901 gctttctttt gggtatagcg tcgtggacag tcattcatct ttctgcccct ccaaaagcaa
      961 aaacccgccg aagcgggttt ttacgtaaaa caggtgaaac tgaccgataa gccgggttct
     1021 gtcgtggaca gtcattcatc taggccagca atcgctcact ggctcaagca gcctacccgg
     1081 gttcagtacg ggccgtacct tatgaacccc tatttggcct tgctccgggt ggagtttacc
     1141 gtgccacgga ctgttaccag ccgcgcggtg cgctcttacc gcaccctttc acccttacct
     1201 gatcccgctt gcacgggcca tcggcggttt gctctctgtt gcactggtcg tgggtttccc
     1261 ccccaggcgt tacctggcac cctgccctat ggagcccgga ctttcctccc ctccgcccgt
     1321 ctcccccgaa gaggacgacg acgaagcggc gactgtctgg tcagcttcgg cgcgcagtat
     1381 agagggtttg cgcgcccttg tcaccccgtg ttgcgcattc caatcgccag tgtcgcggca
     1441 atattacgtg aagcacggca gatattgtca taagccccgc ggaatgcttc gtccaacgta
     1501 cctatgctgg tcaatacgct gaagaccgca tcaatgccat gctgatgtac aacgccaaca
     1561 tcatcggtca ggctacccgc aatgccaatc accggtttat ggtacttctt cgccacgttt
     1621 gcgacgccaa tcggtacctt gccgtgaata ctctggctgt caatacgccc ttcaccggtg
     1681 atcaccagcg tacaatcgtg aatatgttcc tccagattca gcgccgtagt gacgatttca
     1741 ataccacttt tcagttccgc accaagaaac gccatcagca ccgcacccat accgcccgca
     1801 gctcctgcgc cggggacatc ttttacatca acatgcagcg cttttttaat gacatcggca
     1861 tagtgagaga ggttattgtc cagctcaaca atcatcgctt cactggctcc cttttgtggg
     1921 ccaaagatgc gcgatgcgcc gttatcgccc accagcggat tggtgacatc acaagcgacg
     1981 cgaatgacgc aatcttttaa gcgcggatcg aggccggaaa tatcaatatc attcagagta
     2041 ttaagactac cgccgccaaa accaatttca ttgccgttgg cgtcgcataa tttcgccccc
     2101 agcgcctgta ccatgcctgc gccgccatca tttgtagcgc taccgccaat gccgataata
     2161 atgtttgtcg cgccgctctc cagcgcctac aggattaact cgcctgtgcc gcgtgaagtg
     2221 gtcacgagtg gatcgcgttt ttccgcaggt accagctcca gcccactggc cgccgccatt
     2281 tcaataaacg cggttttgcc atcgccggag atcccccaac tggcattcac tttctcgccc
     2341 agcggccctg taacccaggc gtgacgttca gaaccctggg tggctgcaat catcgcttcc
     2401 accgttcctt cgccaccgtc ggcaaccgga atagaaacgt actgtgcatc aggaaaaatt
     2461 tcccgaaatc ctttttctat cgcctgcgca acctcgctgg cagataaact ttctttataa
     2521 gagtctgggg cgattacgat tttcatacct atgcctgtta ccacatgaca ccggagggcg
     2581 tttctcttat tcggcctgga ttccaggccc ggattgcaat acgccatccg ggcacgacgt
     2641 cattaacgag taacttcgac tttcgccagt ttttcgtagt agcacgccag ggcgctatga
     2701 tccgccgttc ctaaaccatc tgctcgcagt gcctgcatca tctccataac cgcagctgtg
     2761 agcggcagtt gcgcgccgac gccgtgggaa gtatccagcg catttgccag atccttaata
     2821 tgcagatcaa tacggaagcc cggcttgaag ttgcggtcca tcaccatcgg cgctttggca
     2881 tccagcacgg tactgcccgc cagtccaccg cgaattgcct gatacaccag gtccgggtta
     2941 acgcccgctt tagttgccag cgttaacgct tctgacatcg cggcaatatt cagcgccaca
     3001 atgacctgat tcgccagttt ggtgacgtta cctgcaccaa ttgccccggt atgcaccacg
     3061 gaacccgcca ttgctttcat caaatcatag tatttgtcga aaatagcctt gtcgccgccc
     3121 accatcactg acagcgtacc gtcgatggct ttcggttcac cgccgctcac cggagcatcc
     3181 agcatatcaa tgcctttcgc tttcagcgct tcgctgattt cacggcttgc cagcggtgcg
     3241 atagaactca tatcgatcaa taccgtacct ggcttcgcgc cttcaataat gccattctca
     3301 cccagcgcca cctctttcac atgaggggag tttggcagca tggttatgat gacgtcgcac
     3361 tgttcagcga tcgctttagc cgtagaggct gtttctgcac ctgcagcaat cacgtcagca
     3421 atagcttctg ggttacggtc agcaaccacc agcgagtaac ctgctttcag aaggttttta
     3481 ctcattggtt tacccataat ccccaggcca ataaaaccaa ctttcatagt catatcaatc
     3541 atctctcttg ttgcggtggt ggttattttt taaaggtatc agccagttta tgagtggcag
     3601 agcggaagac gccgagatcg ctgccgacag ccacaaacgt cgcgccccat tccagataac
     3661 gacgcgcatc ggcttcgacc ggcgcgagga taccgctggg tttgccgtgc gcgctggcac
     3721 ggttaaaaat gtgctgaatt gctttttgta catccgggtg tgatgcattg ccgagatggc
     3781 ctaatgccgc ggccagatcg ctggggccga cgaagatgcc gtctacgcct tcggtagcgg
     3841 caatggcatc gacgttatct acgccctgct gactttctat ctggaccaga atagtgatgt
     3901 tcttgttcga ctgagcgaaa taatccgcca cggtgccaaa catattggcg cggtgagaaa
     3961 cggagacgcc gcgaatgcct tccggtgggt aacgggttga tgccaccgcc tgctctgctt
     4021 cctcttttgt ttctacaaaa ggaatcagga agttatagaa accgatatcc agaagacgct
     4081 taataatttc cggctcgttg gtcggcactc acactactgg cgcgctggcg ctgcctttca
     4141 aggccattaa ctgcggaata aacgtggaga tatcgtttgg cgcatgttcg ccatccagca
     4201 ccagccagtc aaacccagcc aaaccaagaa cttcagtgct aatcgggtta gagagtgctg
     4261 accagcaacc aatttgtacc tgtttcgcag ccagtgcggc tttgaattta ttcgggaaaa
     4321 catcgttatt catcgcttat acctttgctt atttctgcaa ttccatacgt ttaatgtcgc
     4381 caactacgaa gaggtagcag accatcgcca tcagcgctga acatcccacg aaaaccagtg
     4441 ctgcattgaa ggagtgcagt tcacttacca ggtagccaat caccagtgga gtgacaatgg
     4501 aggcaacatt gccaaagacg ttaaagacgc cgccacagag gccaacaatc tctttcggcg
     4561 cggtgtcaga aatcaccggc cagcccagag caccaaatcc tttgccaaaa aaagccagcg
     4621 ccatcagcat gaccaccagc gtggtgttgt tggtgtagtt acataagatg atggtggaag
     4681 ccagcaacat tcccagcaca atcggtagct tacgtgccag ggtcagggat aaaccgcgtt
     4741 tgatcagata atccgagaag acacctccca gcacgccgcc cgcaaaacca cacagtgccg
     4801 gaatcgaggc gaccagaccc actttcagaa tcgacatgcc tttttcctgc accagataaa
     4861 tcgggaacca ggtgaggaag aaccaggtga tggtgttgat aaaatattgt ccgaaaaata
     4921 cgcccaacat catgcggtta gagagcaatt gcttgatgta atgcagtttg agaccgcttg
     4981 ctgccgcgct gcccggcttt ttgtggtcca tatcgaccac cgcgccattt tcagagataa
     5041 acttcagctc ttcctcagac atacgtgggt gatctgtcgg gttatgaatc aacttgatcc
     5101 acagcgccgt cagcacaaaa caaatcaccc ccataacggt aaagacgtgc tcccatcccc
     5161 aggcgaaagt cagccagcca agcagaggcg aaaagagcgc cagcgagaaa tattgtgccg
     5221 agttaaagat ggcggaggca gtaccacgtt ctttcgtcgg gaaccaggcg gcgacaatac
     5281 gggcgttcgc cgggaatgat ggagcttccg agaatcccag cataaagcgc ataaagaaca
     5341 tggagatccc tgcccaggcc agcgggaaca tatcaacaaa gccttgcagg aaggtgaaca
     5401 gcgaccagaa aaagaggctg taggtgtaaa cttttttcga gccaaactta tcaagcaacc
     5461 agccgccggg gatttgcatc agcaagtagg cccagccaaa agcggagaag atgtaaccca
     5521 tcgaaaccgc acttaactgc aactcttttg ccacttcggt accagcaata gacagcgttg
     5581 cacgatcggc gtagttaacg gcggtaacaa taaaaataat cagtaatatt aaatagcggg
     5641 tatgcacacc tttccttttt tcgtcaacgg tgtccagaat cattttattt acctcgggta
     5701 cttatgctga tttttattat tatggggaag gtgttattta taagtttcat ttatgccgta
     5761 acgacaatga actcgggaat tagtataagc agcgcgagaa taataatcat tgtgcaaatg
     5821 ctaatttaat taatactttt taaatattat tttgagcata tgcacataag gttgcgcgct
     5881 aaagcacaga tttgcgcttt accttgccgg gcggcactgc aatccctgaa atgattgaca
     5941 ttgatcacat ttctgcgttt aaactcctga cattcttatt tcacccaatg aaatcattta
     6001 tttttaaatg agaccaggtc ctcattttaa taacccctgg ctggagaata ttgcacaatg
     6061 gccaacatcg aaatcagaca agaaacgcca actgcgtttt atataaaagt tcacgacaca
     6121 gataatgtgg caattattgt taatgataat ggcctgaaag caggaacgcg ttttccggat
     6181 gggctggaat taattgaaca tattccccag gggcataaag tcgcattgct ggacattccg
     6241 gctaatggtg aaattattcg ttatggcgaa gtgattggtt acgccgtgcg tgcaatccca
     6301 cgcggaagct ggatcgacga atcaatggtt gtactaccgg aagcgccgcc gttacacacg
     6361 ctgccactgg caaccaaagt cccggaaccc ttaccgccgc tggaaggata cacctttgag
     6421 ggctatcgca atgccgatgg cagcgtgggc accaaaaacc tgctcggcat caccaccagc
     6481 gtccactgtg tggcaggcgt ggtggactat gtagtaaaaa tcattgaacg cgatctgcta
     6541 ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc atttgtacgg ttgtggcgtg
     6601 gcgattaacg caccggcagc agttgtacct attcgtacca ttcacaatat ttcgctgaac
     6661 cctaactttg gcggcgaagt aatggtgatt ggcctgggtt gtgaaaagtt gcagcctgag
     6721 cgcctgctga ctggaacgga tgatgtgcaa gctattccag tagaaatcgc cagcattgtc
     6781 agtttgcagg atgaaaagca tgtcggtttt cagtccatgg tcgaggatat tttgcaggtc
     6841 gccgaacgcc atctacaaaa actgaatcaa cgtcagcgag aaacctgtcc ggcttcagaa
     6901 ctggttgtcg gcatgcattg cggtggcagc gatgcgtttt ctggcgtaac ggcaaacccg
     6961 gcagttggct atgcgtctga tctactggtg cgctgcggcg caacggtgat gttttcagaa
     7021 gtcacggaag tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc
     7081 ggcaaacggc tgctggagga gatggagtgg tacgataact atctcaatat gggaaaaacc
     7141 gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg gtctggcaaa cgtggtagag
     7201 aaagcgctcg gctccattgc taaatcgggc aaaagtgcaa ttgttgaagt gctgtcgccc
     7261 ggtcaacgcc cgactaaacg cggattaatt tacgccgcga cgccagccag cgattttgtc
     7321 tgtggcacgc aacaggtggc ttcgggtatc accgtgcaag tgtttacgac cggtcgtggt
     7381 acgccgtacg gcctgatggc ggtacccgtc attaaaatgg caacccgcac cgagctggcg
     7441 aaccgctggt ttgatttaat ggatattaat gcgggcacca tcgctaccgg cgaagaaact
     7501 attgaagagg tgggctggaa gttgttccac tttattctcg acgtcgccag cgggaagaag
     7561 aaaaccttct cggatcaatg gggactgcat aaccagctgg cggtgtttaa cccagcaccg
     7621 gtgacctgat aattcaattc gtattatgcc cgatagcaca cgtttatcgg gcatattgag
     7681 tcacagcgaa gacgcgtgat ccccgaccag aatcacttca accccagcct ttcgcagtcc
     7741 ttccagacta tccgcaggaa tgccttcatc aacaatgatc atgtcgatac gttgagtatc
     7801 aatgatctta tgtaaactgg aacgattgaa tttactggaa tcggtgacca cgatgatccg
     7861 ttccgcaact tcgcacatcc gacggtttaa acgggcttca tcttcattat gtgtgctgac
     7921 gccgcgctcc agatcgatcg catctacacc aagaaacagc atatcgaagt ggtaattttg
     7981 cagcgattgc tcagcctgat cgccgtaaaa agattgcgac tgacggcgca aatgcccgcc
     8041 ggtcatcagc agctcaacgc cttccgcttc cagcaacgca ttagccacgt tcataccgtt
     8101 ggtcatcgca attacgtcag tgtacttgcg catcagacga gcaatctcaa aagtggtggt
     8161 cccggaatcg aggatcaccc gatgacctgg ctgaatcaac tcaacggcag ctttcgcaac
     8221 gctgcgtttc atcgcggtgt tcagtgcgct tttatcttcc actgatggct cgactgacgg
     8281 cgtcgtgcta tcgcagatca acgcgccacc ataggcacgc acagcgatcc cctgcttttc
     8341 cagaaacgcc agatcattgc ggatcgtcac agtagatacg ccatacaatg ccgacagatc
     8401 gttaacctgc acactccctt gctgtcgcag acgctgaatg atctgttctc gtcgctcgct
     8461 ggtgcctgtc actcgcttct cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt
     8521 aaaactttcg tttcatttcg ttttgcctat taacgccttt ctattaagca aatgcaagcc
     8581 caccttgccc attggcgcaa gctactctcg tttcagtgac tttcattacg tttcttttgt
     8641 gaatcagatc agaaaactat tatctttcgt tttattttta tctcaccatg acgcagtatc
     8701 aattgaaaca aaacgaaaga tcaatatcgc agtaatctga actggagagg aaagtgaaac
     8761 atctgacaga aatggtgaga cagcacaaag cgggcaaaac aaatgcaatt tatgccgttt
     8821 gttccgcaca tccgctggtg ctggaggctg caatccgcta cgccagtgca aaccaaacgc
     8881 cgttactgat tgaagcaacc tccaatcagg tagaccagtt cggcggttat accggaatga
     8941 cgcccgccga ttttcgcggc tttgtttgtc agctcgccga ctcgttgaat ttcccgcagg
     9001 atgcgctgat tctgggtggt gaccatctgg ggccaaaccg ctggcaaaac ctgccagccg
     9061 ctcaggcaat ggccaatgcc gatgatttga ttaaaagcta cgttgcggca ggattcaaaa
     9121 aaatccacct tgattgcagc atgtcctgtc aggacgatcc aattccctta actgatgaca
     9181 tcgtggctga acgcgccgcc cgtctggcga aagtggcgga agaaacctgt cgcgaacact
     9241 ttggcgaagc cgatctggag tatgtcattg gtaccgaagt gccggtacct ggcggcgcgc
     9301 atgaaacctt aagcgagctg gcggtcacca cgccggatgc cgcccgcgcc acgctggaag
     9361 cccatcgtca cgcctttgaa aagcaaggtt tgaatgccat ctggccacgc attattgccc
     9421 tggtggttca acccggcgtc gaattcgatc acaccaacgt tattgattat cagcccgcca
     9481 aagcgagcgc cttaagccag atggtcgaaa actacgaaac gctgattttc gaagcgcact
     9541 ctaccgatta tcaaacgccg caatctctgc gccagctggt gattgaccac tttgccattc
     9601 tgaaagttgg cccggcgctg accttcgccc tgcgtgaagc ccttttttct ctggcggcga
     9661 ttgaagaaga actggtacca gcgaaagcct gttccggtct gcgtcaggtg ctggaaaacg
     9721 tgatgctcga ccgcccggaa tactggcaaa gccactacca cggtgacggc aacgcgcgtc
     9781 gtctggcgcg tggttacagc tactcagatc gcgtgcgcta ttactggccg gacagccaga
     9841 ttgatgacgc tttcgctcat ctggttcgta atctggcgga ttcaccaatt ccgctaccgc
     9901 tcatcagcca gtatctgccg ctgcagtacg tgaaagttcg ctccggcgag ctgcaaccga
     9961 cgccacggga actcattata aaccatattc aggacatcct ggcgcagtac cacacagcct
    10021 gtgaaggcca ataagcaaaa caaagaggaa catgctatgc caaatattgt tttaagccgg
    10081 attgatgaac gcttgattca cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca
    10141 aatctggtgc tggtagccaa cgatgaggtt gccgaagatc cggtacaaca aaacctgatg
    10201 gaaatggtac tggcagaagg gatcgccgta cgtttctgga cgctgcaaaa agttatcgac
    10261 aacattcatc gcgccgccga tcgacagaaa atcctgctgg tttgtaaaac acccgccgat
    10321 ttcctgacgc tggtgaaagg tggcgttccg gtgaatcgca ttaacgttgg caatatgcac
    10381 tacgccaatg gcaaacaaca aatcgccaaa acggtttctg tggatgcggg cgatatcgca
    10441 gcatttaacg acctgaaagc cgctggggtg gaatgcttcg ttcagggcgt cccgacagag
    10501 cctgctgtgg acctctttaa attactttga gggattcatc atggaaatca gcctgttgca
    10561 ggcatttgcg ttgggcatta tcgcctttat cgctggccta gatatgttta acggcttaac
    10621 ccatatgcac cgcccggtgg tcctcggccc gttggtcggg ctggtacttg gcgatctgca
    10681 taccggaatt ttaaccggcg atacgctgga actagtgtgg atggggctgg ccccgctggc
    10741 gggcgcacag ccgcctaacg tgattatcgg tactatcgtc ggcacggcgt ttgccattac
    10801 tactggcgtg aaacccgatg tcgcggtcgg tgtcgccgta cctttcgctg tcgcagtaca
    10861 gatggggatt accttcctgt tctcggtgat gtctggcgtg atgtctcgct gcgaccggat
    10921 ggcggagaac gccgatactc gcggcattga acgcgtgaac tatctggcac tgctggcact
    10981 cggcactttc tattttctct gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa
    11041 aaccatcatt gatgtcctgc cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat
    11101 catgccagca atcggttttg ccgtgctgct gaaaatcatg atgaaaaacg tctacatacc
    11161 ctacttcatc ctgggctttg ttgccgcaac ctggctcaag ttaccggtgc tggctattgc
    11221 tgccgccgcg ctggcgatgg cgctgatcga tctgctgcgt aaatctcctg aaccgactca
    11281 acctgcggca cagaaagagg aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg
    11341 tcagcgaaaa tgaagaaaca ctgctgactg gcgtcaatga aaacgtgtat gaagatcgga
    11401 gcattgccgc ggagctgacg aaaaaagata tcaaccgcgt cgcctggcgt tccatgctgt
    11461 tacaagcgtc ttttaactac gaacgtatgc aggcttctgg ctggttgtac ggtctgctgc
    11521 ctgctctgaa aaagatccac actaataaac gcgacctggc gcgcgccatg aaggggcata
    11581 tgggtttctt caatacccat ccgtttctgg tgacgtttgt tatcggcatt atccttgcga
    11641 tggagcgttc taagcaggac gttaacagta ttcagagcac caaaattgcc gtcggtgcgc
    11701 cgcttggcgg gattggcgat gcgatgttct ggctaacgct actgccgatt tgtggcggga
    11761 taggcgcaag cctcgcgcta caaggctcta ttcttggcgc agtcgtcttt attgtgctgt
    11821 tcaacgtggt acacttgggg ctgcgttttg gtctggcgca ttatgcttac cgcatgggcg
    11881 tggcggcgat tccccttatt aaagcgaata ccaaaaaagt cggccatgct gcgtcaatcg
    11941 ttggaatgac ggtaatcggc gcactggtgg caacctatgt tcgtttaagc accacgctgg
    12001 aaatcacggc gggcgacgca gtagttaagt tacaggctga tgttatcgac aaactgatgc
    12061 ctgccttctt accgctggtc tacaccctga ccatgttctg gctggtacgc cgcggctaga
    12121 gtccgctgcg cctgattgcg gttaccgtgg ttctagggat cgtcggtaaa ttctgccatt
    12181 tcctttaaat acaatgaggt attagatgtt aagtattatt ttgacagggc atggcgggtt
    12241 tgccagtggc atggaaaaag caatgaagca aatcctcggc gatcaatcgc agtttattgc
    12301 catcgatttt cccgaaacct caagcgccgc gctgcttaca tcgcagcttg aagaggccat
    12361 cgcccaactg gattgtgaag acggcattgt ttttctgacg gatttgctgg gcggcacgcc
    12421 gtttcgcgta gcttcaacgc tggcgatgca aaaaccgggc tgtgaagtga ttaccggcac
    12481 caatttgcaa ctgctactgg agatggtgct ggagcgcgaa gggttaagcg gtgaagaatt
    12541 ccgtgtacag gcgctggagt gtggacaccg agggctgacc agtctggtgg acgagttagg
    12601 ccgctgccat gaagaatgtc cggtcgagga aggaatatga cacacgttct gcgcgccaga
    12661 aggctgctga ctgaagaggg atggttcgat gaccatcagt tgcgtgttgc tgacggtgtc
    12721 atcgcagcaa tcgaaccgat tccagtgggc gtgactgaac gcgatgcgga actgctctgc
    12781 cccgcttaca tcgacaccca tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg
    12841 ccggatgtac tcgacaagct ggcaatgcac aaggcacgcg aaggtgtcgg cagttggtta
    12901 ccgaccaccg taaccgcgcc gcttaatacc attcatgcgg cgctgaaacg tattgctcaa
    12961 cgttgccaac gcggcggacc tggtgcgcaa gtgctgggga gttatctcga aggaccgtac
    13021 ttcacgccgc agaataaagg cgcgcatcc
//
LOCUS       DAECNO010000104        13015 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.104, whole genome shotgun sequence.
ACCESSION   DAECNO010000104 DAECNO010000000
VERSION     DAECNO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.104"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..319)
                     /locus_tag="KKO08_003238"
     CDS             complement(<1..319)
                     /locus_tag="KKO08_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBD6319380.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="KKO08_003239"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="KKO08_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KKO08_003240"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="KKO08_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBD6319381.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KKO08_003241"
                     /pseudo
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="KKO08_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="KKO08_003242"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="KKO08_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBD6319382.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="KKO08_003243"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="KKO08_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBD6319383.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="KKO08_003244"
                     /pseudo
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="KKO08_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="KKO08_003245"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="KKO08_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="KKO08_003246"
     CDS             10613..11503
                     /locus_tag="KKO08_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBD6319384.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="KKO08_003247"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="KKO08_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBD6319385.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12493..12569)
                     /locus_tag="KKO08_003248"
     tRNA            complement(12493..12569)
                     /locus_tag="KKO08_003248"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13015
                     /locus_tag="KKO08_003249"
     CDS             12812..>13015
                     /locus_tag="KKO08_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBD6319386.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3183 a         3373 c         3307 g         3152 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccacttct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aagggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg ccgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg tgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctagcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc accctcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc ctggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct agcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat accctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcactgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagtaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcgg ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctggcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgag aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctg
//
LOCUS       DAECNO010000105        12908 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.105, whole genome shotgun sequence.
ACCESSION   DAECNO010000105 DAECNO010000000
VERSION     DAECNO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.105"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KKO08_003250"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="KKO08_003250"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBD6319387.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="KKO08_003251"
     CDS             complement(1806..3224)
                     /locus_tag="KKO08_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBD6319388.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="KKO08_003252"
     CDS             complement(3378..3722)
                     /locus_tag="KKO08_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBD6319389.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KKO08_003253"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="KKO08_003253"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBD6319390.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="KKO08_003254"
     CDS             complement(4712..5647)
                     /locus_tag="KKO08_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBD6319391.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="KKO08_003255"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="KKO08_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBD6319392.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="KKO08_003256"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="KKO08_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBD6319393.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KKO08_003257"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="KKO08_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBD6319394.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="KKO08_003258"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="KKO08_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBD6319395.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="KKO08_003259"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="KKO08_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBD6319396.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="KKO08_003260"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="KKO08_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBD6319397.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KKO08_003261"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="KKO08_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBD6319398.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="KKO08_003262"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="KKO08_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBD6319399.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="KKO08_003263"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="KKO08_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBD6319400.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3401 a         3296 c         2951 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagatactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcgga aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccaag
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgaaaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctact
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAECNO010000106        12801 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.106, whole genome shotgun sequence.
ACCESSION   DAECNO010000106 DAECNO010000000
VERSION     DAECNO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.106"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..351)
                     /locus_tag="KKO08_003264"
     CDS             complement(<1..351)
                     /locus_tag="KKO08_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6319401.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKI"
     gene            727..2367
                     /gene="trg"
                     /locus_tag="KKO08_003265"
     CDS             727..2367
                     /gene="trg"
                     /locus_tag="KKO08_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBD6319402.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2405..3328)
                     /locus_tag="KKO08_003266"
     CDS             complement(2405..3328)
                     /locus_tag="KKO08_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6319403.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3545..4888
                     /locus_tag="KKO08_003267"
     CDS             3545..4888
                     /locus_tag="KKO08_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD6319404.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5113..6768
                     /gene="opgD"
                     /locus_tag="KKO08_003268"
                     /gene_synonym="mdoD"
     CDS             5113..6768
                     /gene="opgD"
                     /locus_tag="KKO08_003268"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBD6319405.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6908..7132
                     /locus_tag="KKO08_003269"
     CDS             6908..7132
                     /locus_tag="KKO08_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBD6319406.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7195..7731
                     /gene="rimL"
                     /locus_tag="KKO08_003270"
     CDS             7195..7731
                     /gene="rimL"
                     /locus_tag="KKO08_003270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBD6319407.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7726..8706)
                     /locus_tag="KKO08_003271"
     CDS             complement(7726..8706)
                     /locus_tag="KKO08_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319408.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8830..9822
                     /gene="tehA"
                     /locus_tag="KKO08_003272"
     CDS             8830..9822
                     /gene="tehA"
                     /locus_tag="KKO08_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBD6319409.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9819..10412
                     /gene="tehB"
                     /locus_tag="KKO08_003273"
     CDS             9819..10412
                     /gene="tehB"
                     /locus_tag="KKO08_003273"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBD6319410.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10715..11383
                     /locus_tag="KKO08_003274"
     CDS             10715..11383
                     /locus_tag="KKO08_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBD6319411.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11401..11907)
                     /gene="tnpA"
                     /locus_tag="KKO08_003275"
                     /pseudo
     CDS             complement(11401..11907)
                     /gene="tnpA"
                     /locus_tag="KKO08_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11915..12801
                     /locus_tag="KKO08_003276"
                     /pseudo
     CDS             11915..12801
                     /locus_tag="KKO08_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3335 a         3004 c         3202 g         3260 t
ORIGIN      
        1 aatcttgagc gcgcgtaaac cagcttctcc gcgctcttca tagaccttca gccacctggc
       61 tacagaacca ctaccagcaa gcataaagtg agcagcagcc tgattaaggg acatgtgctg
      121 ctcgatcaca gctttcacga ccttaatacg caactctgga tcagcactaa cgcctttagg
      181 tttgggaatt aaaccttttt ctccatgttt ttcatagagg gcaacccatg tcctgacctg
      241 ggttcggggg acaccaaaac gtgccgagat gatcctgtaa ccatcatcag tagtgaagta
      301 gtgattcacg acttcaaggc gcttttcaaa agggtatttt ggctttgaca tattaggggc
      361 tattccattt catcgtccaa caaaatgggt gcagtacagt tgatagaaat atcggtcggg
      421 gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc acccgcaggc
      481 atcctatgag gtttccttag ggacgaaaat aatcacttca cgaaattgcg tgctgttttc
      541 cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct tcattccaat
      601 agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg tttttggtca
      661 taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg acgagagaca
      721 acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat caggctggtt
      781 ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag ttctgctctg
      841 gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc ggagattgag
      901 attcggaccg ggttagcgaa cagttcagat tttttgcgtt cagcccggat caatatgatt
      961 caggccgggg ctgcgagtcg tattgcggaa atggaagcaa tgaagcgaaa tattgcgcaa
     1021 gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa tcgaccggtg
     1081 aaaacacctg ctgatgaagc cttcgacact gaattaaatc aacgctttca ggcttatatc
     1141 acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc gattatcaat
     1201 catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt gaacaaagcc
     1261 gttaagatac gtagcaccag agccaaccaa ctggcggaac tggcccatca gcgcacccgc
     1321 ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac gctgataaca
     1381 tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgccgcaca acggattgaa
     1441 aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg cgggtcgtaa tgaaatcggt
     1501 cgcttaagtc gtcatttaca gcaaatgcag cattcactgg ggatgacagt agggactgtt
     1561 cgacagggcg cggaagagat ttatcgtggc accagcgaaa tttcagctgg caatgcggac
     1621 ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac aaactgccgc cagcatggag
     1681 caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag caaactggcg
     1741 caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt agtaaaaacg
     1801 atgggcgcta tctccacgag ttcgaagaaa atttctgaga tcaccgccgt catcaacagt
     1861 attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc gcgagcgggt
     1921 gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc aagccgcagc
     1981 gctcaggcag cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt aattgacctg
     2041 gggtcggatg aggtggcaac ggcagggaaa accatgagca ctattgttga tgccgtcgcg
     2101 agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag tagaggcata
     2161 actcaggtta gccaggcgat ttctgaaatg gataaggtga cgcaacagaa tgcttctctg
     2221 gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac aggctgcacg attaactgag
     2281 gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg cggagcgggt
     2341 gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca gatcccttca
     2401 cctttcagaa cggcattgat tttcgaatag cgttaatcat caactggcaa ccagaagaga
     2461 acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt cccggaacag
     2521 gcagggccac cagcgtggca tgacgcaggt cgtcttttac agcgccagaa gggacaaacc
     2581 acacgtaatc gtattcaacg gtaagttgac gagatagcga agtagacagc gtttcgatac
     2641 aacccgaagg aattttacag ccctggctct gcactaatgc atctgaatgc tggcgtggcg
     2701 cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc gttacgttct
     2761 cctgaagtag cgggtgatta gggcggacaa ccagcttcaa cgattcaaga aacagcagtt
     2821 cgtaattaag cccggtcatc agttcaggat ctgacatccg accaatgccg atatcgattt
     2881 ccccggtttt taaacccgcc agaatcatag ggttactcat tgtcgcaact tgcaaggtcg
     2941 tctctttttg ttgctgatga aactgaccta taaccgaagg taatatcccc agtgccgcag
     3001 taggtagtgc accaaccctg acgacatcat tattaagacc ttctttacga tgaagcgact
     3061 gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat tgttcgccgg
     3121 gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc gtcagctgct
     3181 ccagttcatt caatgtatta gagagcgcag gttgactcaa attaagggtt tcagctgcgc
     3241 gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc aaacggatgc
     3301 gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg gtgtcgctga
     3361 caagtgaagt tgtttgatta tgataacttg attgcaaaat attattaata attaaagcaa
     3421 ttatgttaca gcaaaatgga taatattgat gttttcgcgg cgagatcaca gtttgtaaat
     3481 tcttcccgcc agagtgaatg cggttaccta cactccagat tactgaccac tggaggcaga
     3541 cactatggcg aacagcatca cggcggatga gattcgggaa cagttttcgc aggcaatgtc
     3601 agccatgtac cagcaagaag ttccgcaata tggcacgctg ctggaactgg tagctgatgt
     3661 gaatctggct gtgctggaaa acaatcctca actgcacgaa aaaatggtaa atgcagacga
     3721 gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg cacaagagct
     3781 tgctactctt cggcggatgt ttgccattat ggggatgtac ccggtgagct attacgatct
     3841 ctcgcaggca ggggtgccgg tacattcgac agcatttcgg cccattgatg atgcttctct
     3901 ggcgcgtaat cccttccgcg tttttacatc cttactccgc cttgagctta tcgagaacga
     3961 aattttgcgc cagaaagcgg cggagattct gcgtcagcgc gatatcttca ccccacgttg
     4021 tcgacaactg ttagaggaat atgagcagca gggcggtttt aacgaaacac aggcacagga
     4081 gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag ttagcaacgg tagatgaaga
     4141 aacctatcgc gccttgcaca acgaacatcg gttgattgct gatgtggtct gttttcctgg
     4201 atgccatatc aaccacctga cgccacgtac gctggatatt gaccgggtgc agtcgatgac
     4261 gcctgaatgc ggaattgaac ccaaaattct catcgagggg ccgccgcgcc gcgaggtacc
     4321 gattttacta cgccagacca gctttaaagc actggaagag acggtgttgt ttgcggggca
     4381 gaaacagggc acgcataccg cgcgctttgg tgaaattgag cagcgtggcg tggcattaac
     4441 gccgaaaggg cgacaactgt atgatgatct tctgcgtaac gctggaaccg ggcaggataa
     4501 tctcactcac caaatgcatt tacaggaaac cttccgcact tttcctgaca gtgagttttt
     4561 aatgcgtcag caagggttgg catggttccg gtaccgtctg acgccttcgg gtgaggcgca
     4621 tcgtcaggcg attcatcctg gagacgatcc acagccctta attgaacgtg gttgggtagt
     4681 ggcgcaaccc atcacctatg aagatttctt gcccgttagc gcggcgggga tcttccagtc
     4741 aaatctgggt aatgaaacgc agacacgcag tcacggtaat gccagtcgcg aagcatttga
     4801 gcaggcgttg ggttgtccgg ttttggatga gttccagctt taccaggaag cggaagaacg
     4861 cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc cgcctttgca aaaatttgcc
     4921 tgattttaca aacgaatcag gctcatccca tcgacataaa aaaaatgccg atttatgcat
     4981 attctctcag ttcaacaatt ggattattaa taaatattgt ctagagtgag cggtcataaa
     5041 taagcacttt cttgccgctg aaaacgacca gcgcgggacc attcacaaca ccagaaggac
     5101 tcactttcag gtatggatcg tagacgattt attaaaggtt taatggctat ggccgccgtg
     5161 tgcggtacca gcggcattgc ttctcttttt tctcaggcag cattcgcggc agattctgat
     5221 attgccgacg ggcaaaccca gcgttttgac ttctccattc tacagtcaat ggcgcacgac
     5281 ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct ggcgacaatg
     5341 acgccgcagg cttataacag tattcaatac gacgccgaaa aatcgctctg gcataacgtt
     5401 gagaaccgtc aactggacgc tcagttcttc catatgggaa tgggattccg tcgccgcgtt
     5461 cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg aaattcactt tcgcccggag
     5521 ttgttcaaat acaacgatgc aggtgttgat accaaacaat tagaagggca aagcgatctc
     5581 ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga tgtagtatca
     5641 tttctcggcg cgagttattt ccgcgccgtt gatgatacat atcaatacgg tttgtcggcc
     5701 cgcggcctgg cgatcgacac ttacaccgac agtaaagaag agttccccga ctttaccgcc
     5761 ttctggtttg atacggtaaa accgggggca actaccttta ccgtttatgc gttgctcgat
     5821 agcgccagca ttactggtgc ctataagttc actatccatt gtgagaaaag tcaggtgatt
     5881 atggatgtgg aaaatcacct gtatgcgcgc aaagacatta aacagctggg catttcgccg
     5941 atgaccagta tgttcagctg cggtactaat gaacgtcgga tgtgcgatac aattcatccg
     6001 caaattcatg actctgatcg tctgtccatg tggcggggca acggcgagtg gatttgccgt
     6061 ccgctgaata atccgcaaaa attgcagttc aatgcttaca ccgacaacaa cccgaaaggg
     6121 tttggtttat tgcaactgga tcgtgacttc tcccattatc aggacattat gggctggtat
     6181 aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt ggggtaaggg caccatcggc
     6241 ctgatggaaa tcccaacaac gggcgaaacg ctggataaca ttgtctgctt ctggcagcca
     6301 gaaaaagctg taaaagcagg tgatgagttt gcattccagt atcgtctgta ctggagtgcg
     6361 caaccgcctg ttcattgccc attagcgcgc gttatggcga cgcgtaccgg catgggcggt
     6421 ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg tcgttttgcc
     6481 gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa aaggcattga gccggtgatt
     6541 acgctttcca gtggggaagc gaagcaaatc gaaattctct atattgaacc catcgatggt
     6601 tatcgtattc agtttgactg gtatccgact tcggactcca ctgatccggt cgatatgcgg
     6661 atgtatctac gttgtcaggg ggacgctatc agtgaaacat ggctgtatca gtatttcccg
     6721 ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga tgagttaatc gttttttctt
     6781 cggcaccttc ttcgggaggt gccgtctggt taaacacgat cccgctcgca tttttcccta
     6841 agttaaatga gtaatctgat ggtgtgtatt tcagatacac cgtgtcagcc actaacaggg
     6901 agtgcgtatg tttccagaat accgagattt aatatcccgt ctgaaaaacg aaaatcctcg
     6961 ctttatgtcc ttgttcgata aacacaataa acttgatcat gaaattgcca gaaaggaagg
     7021 ttccgacggt cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa agctacagtt
     7081 aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt aaactttcct
     7141 aagccgccag ccaggcggct tttttaacaa cagcatggat tgactggaga taagatgact
     7201 gaaacgataa aagtaagcga atcacttgaa ttacatgctg ttgcggaaag tcacgtcaca
     7261 cctctttatc agttaatctg taaaaataaa acctggttac agcagtcgct aaactggccg
     7321 cagttcgttc agagtgaaga ggacacgcga aaaacggtgc agggtaatgt gatgttgcat
     7381 caacgcggct atgccaaaat gttcatgatt ttcaaagaag atgaacttat cggcgttatc
     7441 tcgtttaatc gtattgaacc actgaataaa actgctgaaa tcggctactg gctggacgaa
     7501 tctcatcagg ggcaggggat catttctcag gcgctgcagg cattgattca tcattacgct
     7561 cagtctggtg aacttagacg cttcgtgatc aaatgtcggg tggacaatcc gcaaagcaac
     7621 caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc tgagttcctg
     7681 aatgatgcct atgatgatgt gaacttatac gcgcgtatta tcgattcata ataaccctgc
     7741 cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact tttggaccac ggacatgaac
     7801 cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt aactccacat ggttttcaat
     7861 tatcaccgca cctgtaatac ggctctcacc ttgtattaca acgtgctcgt cgagcagaat
     7921 cggtccacca cgtaccacgg catttccgcc aatcaagaca tgatgtttta acacacaatt
     7981 accttccaca atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg gaatggcatc
     8041 ttcttctatg ccagctttca cctgcgcgtg accgtagact ttagcgcaat cgcatagcca
     8101 gacgttgttc tcttcattac cttcaacact ggcaaaatca aaaacttcgg cgcgatgttc
     8161 aataaaagca taccggacga cggcatcgcc ataaatttgt gcctggtgga caatacgcga
     8221 ggcgctaacc cttgcgcgat cataaatttg gagcaggagt tgatggtcgg gcgttaagcc
     8281 ttgtgcggcg acgatcatag aatgttgatc aattaaggcg tgaccaaata ttctacattg
     8341 cacgtaaact aatgagtcat gaatggtgac actgtcactt atatatgcac cctggctgat
     8401 ttcactatta tcgatccaga cgttatccgt tgcgtaaacc tctccccaca gcacgctggt
     8461 gccggtgata cgcgtattac cggaaatcac cgttccgccg aacgcaatag cattctggtc
     8521 gtaaatccag caatttcctt cctgtgctaa caccgtttcg cgatcgatcc agccgccagc
     8581 ggttcctgct atcacatcgt taaaatcgct aatggcgata atctgccgta ataacacact
     8641 ttttttagtg ccatcttcct gataactaaa ggctcgctgt tcttcactta ggcgatattt
     8701 gcgcatagcg ttttcccaca ggtgacttac tataaccgta gcaaattctg cggctctggc
     8761 tatgctcgag aaaattccat aaaatgcatt tcaaatatac tttataaatt aaacaaaatg
     8821 agtaagaaga tgcagagcga taaagtgctc aatttgccgg caggctactt tggtattgtg
     8881 ttggggacga tagggatggg atttgcctgg cgctatgcca gccaggtttg gcaggtcagc
     8941 cactggttag gggatgggct ggtgattctg gcgatgatca tctggggatt attgactagc
     9001 gcatttatta cccgactcat acgctttccg catagcgtgc tggcggaagt tcgccatcca
     9061 gtgctgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc gattggtttt
     9121 gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt ttggtgtcgt ggttcagttg
     9181 gcttatgccg cctggcaaac tgcgggatta tggcgcggat ctcaccctga agaagctacc
     9241 acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat ggcctgtggt
     9301 gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt ctcatggcta
     9361 agccttgaac cggtgatctt gcagcgtctg cgcagttcgg gagaattacc cacggcactg
     9421 cggacatcac tcggcattca gctcgctcct gcgctggtgg cctgtagtgc ctggctgagc
     9481 gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt tcggttatgg actgctgcaa
     9541 ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa tgcttcattc
     9601 tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct ggggagtggc
     9661 agcgataatg gatttttcca tacgctggcg gtgccgctgt ttatctttac caattttatt
     9721 attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt gttagtcaga
     9781 accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt gacgaaaact
     9841 attttactga taaatatgaa ttaacccgca cacactctga agtactggaa gcggtgaaag
     9901 tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac agtctttacc
     9961 tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt atcgccaacg
    10021 tcgagcgcat taaatccatt gaaaatctgg ataatttaca cacccgggtc gttgatctga
    10081 ataacctcac atttgatgga cagtacgatt ttattctttc gactgtggtg ctgatgttcc
    10141 ttgaggctaa aaccatccca gggttgattg ccaatatgca acgttgcact aaacctggtg
    10201 gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc gtcggcttca
    10261 cgtttgcctt caaagaggga gaattacgtc gatattacga aggctgggag agggtgaaat
    10321 acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt attaaactgc
    10381 gtttcgccac gatgctggca cgtaaaaaat gacccggtaa gcacaaaacg cgtgaaaatt
    10441 ccccacgctg agatgattta ctgttcttct tttcggtaag catatttttt atcgaaggga
    10501 tgtgaaatta atcacagtag tcgaagtttt ttagcagctt aacttactga aatttaacta
    10561 ctgatgattg acttagcccc tttttcggca ttgactatgt cgtctgaaaa ggggctgaaa
    10621 aatttatttt caccaacact ttttttgcca caacacgaag cggcgctttt tgctataact
    10681 tagaaagtaa tataatcatc tcaggaaact attcatgcgt accacatcat ttgcgaaagt
    10741 tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca cccagccaga
    10801 taaatattct ggttttttaa acaattactc tgatttaaaa gaaacaacct cagcaacagg
    10861 taaacctgtt ttacgttggg tagacccgag ttttgatcaa agcaaatatg acagcatcgt
    10921 ctggaaccca atcacttatt atccggtacc aaaaccttct acccaggtag ggcagaaagt
    10981 tctggataaa attttgaact ataccaacac cgaaatgaaa gaagcgatag cgcagcgtaa
    11041 accagtggtt accaccgctg ggccgcgtag tctgattttc cgtggggcca ttaccggtgt
    11101 agataccagc aaagaagggc tgcaattcta tgaagtggtt cctgttgcat tagtggttgc
    11161 ggggacgcaa atggctacag gccaccgtac catggatact cgcctctatt ttgaaggtga
    11221 gctgattgat gcagcgacta ataaaccggt tatcaaagtc gttcgtcagg gcgaaggtaa
    11281 agacctgaat aacgaaagta cgccaatggc tttcgaaaat attaaacaag ttattgatga
    11341 catggcgacc gatgccacca tgtttgacgt taacaaaaag tagttccaga cgcgccatcg
    11401 ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat ccttcagggc ggggatataa
    11461 ggcgcggttt tccacctgac caggtgtttg ctgttgttca atgtattggc ggatgacgga
    11521 tattggcgca cctccgcagc tactggcaaa atagccagga ctccacaaaa cgcctttgta
    11581 gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt aacctacccg atacgccttt
    11641 gaggctgttt accagactgg atatcgccag tttgggaggt tagttaatta acaaatggac
    11701 gtgatctggt tcgccatcca tttcaaccag ttcagcttca aaataagcac atacatttga
    11761 aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa atctggcgtc ggtatctggt
    11821 gacaaagact aggtgaacat gcatcaggaa aacacaatgc cttccacgcc ggatatcggt
    11881 ttcttttttc acagaccaaa gtatcatttg acctgtgaaa cgattacagg catttaaatt
    11941 ccagttaaga ccaggtggtc aacaggagtg tgaaatgagg cgcttcgcag gcgcatgtcg
    12001 tttcgttttc aatcgtgctc tggcacgtca gaatgagaat catgaggccg gtaataaata
    12061 catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaatgcca ctgaaacgca
    12121 atggcttaaa gattctccct cacagccatt gcaacagtca ctgaaagacc ttgagcgggc
    12181 ttacaaaaac ttcttccgga agcgggctgc ttttccccga ttcaaaaagc ggtgacagaa
    12241 tgatgcattc cgctacccgc agggcgttaa gctcgatcag gaaaacagcc gtatttttct
    12301 gccgaaactg ggctggatgc gctaccggaa tagccgtcag gtcacgggtg ttgtgaaaaa
    12361 tgtcactgtc agccagtcct gcggtacgtg gtacatcagt attcagacag aaagtgaagt
    12421 atccactccg gctcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact
    12481 cgccacgctg tcagatggca cagtctttga gcctgtaaac agttttcaga aaaaccagaa
    12541 gacgctggcg agacttcagc gacagttaag ccgcaaggtc aaattcagca acaactggca
    12601 gaagcagaaa cgcaaaatac agcgactgca ttcctgtatc gcaaatatcc gcagggacta
    12661 ccttcacaaa gtcacaacgg ccgtcagcaa aaaccacgca atgatagtca ttgaggattt
    12721 gaaggtcagc aacatgtcaa agtcagcagc gggtacggtc agccagccgg ggcgcaatgt
    12781 ccgggcaaaa tcaggtttaa a
//
LOCUS       DAECNO010000107        12695 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.107, whole genome shotgun sequence.
ACCESSION   DAECNO010000107 DAECNO010000000
VERSION     DAECNO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.107"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="KKO08_003277"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="KKO08_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBD6319412.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="KKO08_003278"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="KKO08_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBD6319413.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="KKO08_003279"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="KKO08_003279"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBD6319414.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="KKO08_003280"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="KKO08_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBD6319415.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="KKO08_003281"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="KKO08_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBD6319416.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="KKO08_003282"
     CDS             4025..4615
                     /locus_tag="KKO08_003282"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBD6319417.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="KKO08_003283"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="KKO08_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBD6319418.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="KKO08_003284"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="KKO08_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBD6319419.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="KKO08_003285"
     CDS             complement(6551..6658)
                     /locus_tag="KKO08_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319420.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="KKO08_003286"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="KKO08_003286"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBD6319421.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="KKO08_003287"
     CDS             7648..7875
                     /locus_tag="KKO08_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBD6319422.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="KKO08_003288"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="KKO08_003288"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBD6319423.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="KKO08_003289"
     CDS             complement(9447..10703)
                     /locus_tag="KKO08_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBD6319424.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="KKO08_003290"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="KKO08_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBD6319425.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="KKO08_003291"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="KKO08_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBD6319426.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="KKO08_003292"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="KKO08_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBD6319427.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3034 a         3244 c         3197 g         3220 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcggtc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt aacgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 ggctctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAECNO010000108        12416 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.108, whole genome shotgun sequence.
ACCESSION   DAECNO010000108 DAECNO010000000
VERSION     DAECNO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.108"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1200
                     /gene="edd"
                     /locus_tag="KKO08_003293"
     CDS             <1..1200
                     /gene="edd"
                     /locus_tag="KKO08_003293"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBD6319428.1"
                     /translation="MALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVH
                     PDSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMH
                     LVAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAG
                     LLHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKV
                     LSGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPK
                     ANGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKV
                     RDGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQ
                     GATCITF"
     gene            1237..1878
                     /gene="kdgA"
                     /locus_tag="KKO08_003294"
                     /gene_synonym="eda"
     CDS             1237..1878
                     /gene="kdgA"
                     /locus_tag="KKO08_003294"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBD6319429.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1934..3112)
                     /gene="purT"
                     /locus_tag="KKO08_003295"
     CDS             complement(1934..3112)
                     /gene="purT"
                     /locus_tag="KKO08_003295"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBD6319430.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3246..3536
                     /gene="yebG"
                     /locus_tag="KKO08_003296"
                     /pseudo
     CDS             3246..3536
                     /gene="yebG"
                     /locus_tag="KKO08_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3603..3959
                     /locus_tag="KKO08_003297"
     CDS             3603..3959
                     /locus_tag="KKO08_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBD6319431.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4286..4945
                     /locus_tag="KKO08_003298"
     CDS             4286..4945
                     /locus_tag="KKO08_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBD6319432.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5155..7215
                     /gene="ptrB"
                     /locus_tag="KKO08_003299"
     CDS             5155..7215
                     /gene="ptrB"
                     /locus_tag="KKO08_003299"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBD6319433.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7212..7874)
                     /gene="exoX"
                     /locus_tag="KKO08_003300"
     CDS             complement(7212..7874)
                     /gene="exoX"
                     /locus_tag="KKO08_003300"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBD6319434.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7898..8554)
                     /locus_tag="KKO08_003301"
                     /pseudo
     CDS             complement(7898..8554)
                     /locus_tag="KKO08_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8656..8886)
                     /gene="holE"
                     /locus_tag="KKO08_003302"
     CDS             complement(8656..8886)
                     /gene="holE"
                     /locus_tag="KKO08_003302"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBD6319435.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9025..9399
                     /gene="yobA"
                     /locus_tag="KKO08_003303"
     CDS             9025..9399
                     /gene="yobA"
                     /locus_tag="KKO08_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBD6319436.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9403..10275
                     /gene="copD"
                     /locus_tag="KKO08_003304"
                     /pseudo
     CDS             9403..10275
                     /gene="copD"
                     /locus_tag="KKO08_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10288..10629
                     /locus_tag="KKO08_003305"
     CDS             10288..10629
                     /locus_tag="KKO08_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBD6319437.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11022..12098)
                     /locus_tag="KKO08_003306"
                     /pseudo
     CDS             complement(11022..12098)
                     /locus_tag="KKO08_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(12064..12345)
                     /locus_tag="KKO08_003307"
     CDS             complement(12064..12345)
                     /locus_tag="KKO08_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBD6319438.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3081 a         3032 c         3137 g         3166 t
ORIGIN      
        1 atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg tactttctac
       61 ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt gccaggctct
      121 tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag
      181 gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat gatcgatgag
      241 aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac taaccacacc
      301 atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga tgacttctct
      361 gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc ggccgatatt
      421 aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct caaagcaggc
      481 ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta tacccttgaa
      541 ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc actcgacagc
      601 aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa agtgttaagc
      661 ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt
      721 gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt tgaagcgggt
      781 ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg
      841 ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg tttcaaaatt
      901 gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc agctatccac
      961 gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt
     1021 cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga actggctgct
     1081 cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg tgaattattc
     1141 agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat cactttttaa
     1201 gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa aacaagtgca
     1261 gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa actggaacac
     1321 gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga agtgactctg
     1381 cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt
     1441 gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga agcgggtgca
     1501 cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac cgaagggact
     1561 attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat ggactacggt
     1621 ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg
     1681 atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc tccggctaac
     1741 taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg
     1801 gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg tgaagctgta
     1861 gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt tttgtagcgt
     1921 ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa
     1981 tggcgtcaac aacactctct gaagtagcca gtaccacccc cagacgacgg ctgccatcaa
     2041 tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc tgcacattat
     2101 caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct gcaggaccat
     2161 actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg gcaaactctg
     2221 agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg gagacctcac
     2281 tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca taaccgccca
     2341 gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc gggctcattt
     2401 gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct actggtgcac
     2461 agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa
     2521 cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga gcgtattccc
     2581 atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg gaagagctca
     2641 tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt
     2701 tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc gccaggcgac
     2761 ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca acattcagtc
     2821 cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc ggcacgatat
     2881 aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc atattaatga
     2941 catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg acggcaatca
     3001 cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca ccggagccta
     3061 ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc ataacgaata
     3121 tcccctgatt aattccgctc gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg
     3181 atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat tatgagaggc
     3241 tgcaaatggc ggttgaagtt taatacgtag tcattcgtga gggcgaagag aaaatgtcgt
     3301 ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg
     3361 acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc
     3421 tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc aaattaccgt
     3481 ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca
     3541 acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa
     3601 acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc gcatcagttt
     3661 tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg
     3721 ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg gcgcgctggg
     3781 cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt ttgcaggaca
     3841 ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa agtgccgata
     3901 ttcattacca ggtcagcgtg gactgcaaag cgggaatggc ggaatatcag cggcgttaat
     3961 ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg tccgtcccag
     4021 aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc aatcagcatc
     4081 aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat cacttgatat
     4141 acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc cccgcttatt
     4201 tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg ctacaggcac
     4261 taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc aatccctgct
     4321 tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca aactgttagt
     4381 gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag cacgtaaact
     4441 tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag cgggtacggt
     4501 gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac cgcagtttgg
     4561 cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc tggtctttgc
     4621 cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc agcaattgcg
     4681 tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg aacaaccgct
     4741 ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg aaatctattt
     4801 cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc tgaatgcact
     4861 gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg atctcgaaca
     4921 gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt tgactatttc
     4981 gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct gcataacatt
     5041 cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt cgcttgcatc
     5101 cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa taacatgcta
     5161 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
     5221 tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta cctgcaacaa
     5281 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
     5341 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
     5401 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
     5461 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
     5521 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
     5581 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
     5641 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
     5701 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
     5761 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
     5821 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
     5881 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
     5941 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
     6001 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
     6061 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
     6121 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
     6181 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
     6241 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
     6301 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
     6361 caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
     6421 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
     6481 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
     6541 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
     6601 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
     6661 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
     6721 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
     6781 aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
     6841 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtggggaaat
     6901 ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
     6961 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
     7021 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
     7081 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
     7141 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
     7201 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
     7261 atgctgttca ggttattaaa taaccagcgc agatagcccg gatagcgttc ggcaacgtcg
     7321 gaaaccgctt tgccacggta tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg
     7381 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
     7441 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccgg cggtgtctgt
     7501 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
     7561 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
     7621 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
     7681 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
     7741 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
     7801 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
     7861 ataatgcgca acatggcatg ctccaggccg aaaaagccta gcgtaatgga atgatatcgc
     7921 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
     7981 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
     8041 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
     8101 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
     8161 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
     8221 tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa
     8281 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag gtcgttgcgg
     8341 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
     8401 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
     8461 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
     8521 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
     8581 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
     8641 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
     8701 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
     8761 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
     8821 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
     8881 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
     8941 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
     9001 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
     9061 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
     9121 cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac tttaaacttc
     9181 tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat
     9241 attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat tgttccactg
     9301 gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg
     9361 cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg
     9421 atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt tgcaatgtat
     9481 ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc tctgcgttta
     9541 caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc
     9601 gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg
     9661 caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc
     9721 gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa
     9781 tttattctgc tggcgggagt ggggcatgcg acgctgaatg aaggggtaac agcgaaaatc
     9841 catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg
     9901 ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc tattcaggcg
     9961 ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg
    10021 cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc
    10081 ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc taatcgttat
    10141 gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg
    10201 actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact
    10261 ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta
    10321 ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag tcgttttgaa
    10381 gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg ccgaccgggt
    10441 aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa cgatatcgca
    10501 caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat
    10561 gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa
    10621 ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt
    10681 caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt tattgaccat
    10741 tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca aggcaactaa
    10801 gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt tccctggacc
    10861 gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt agttgcaaac
    10921 gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt ttttggttat
    10981 atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag taagtacgtt
    11041 ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc aagtagacgc
    11101 tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct tctgatttcg
    11161 tgaaaagttg gtgggttttc actgaacagg atacctgtta attttctggc tgcgacaaat
    11221 ttctttgtca ggccgtccgg gtgaatgctg ccgtcagggc tgttttttct aatcccggca
    11281 ctgattagat aatcccccct acttaccatg cggcactgtt caactactgc accaagtcgt
    11341 aaaccagcga caggaaggct gagtgacagg gggatagcaa tcatcattcc tgtcttaatt
    11401 tgcctgatgt ggagacgatc atcataaata tcactgaacc gcatattcgt tatgtcttcg
    11461 cgacgttgtc ctgttacaag ggctaagtcc atagctaatg ggaaccatgc cggaagttga
    11521 tctgctgcct ccctgatgca gttgtatgtc tttagtttca gccgttctct tgtaactact
    11581 attttcggtg ctcttgttgg cgttactgga ttttgagata tacgtccttc aacaatggct
    11641 tcgcggaaca tatcagataa cacagaacgc attgatcctg ccatcgtgtt tttccctcct
    11701 tcaatctaca aatcaagaaa ctcggcgata tggcgagtgg ttatttctgt cagtaaaacc
    11761 cctcctaatt tttcttttat tgtctccagt tgatttaccc gagttttata agtatttctg
    11821 gacgcttttc tccttataag aatcgttttg taacgttcaa tccagcctgc catagtaaat
    11881 gagtcgaccc ctttaagctt ttcaattagg gcagcaggag agtagttttt gtatatataa
    11941 tgatttgctt caattgcctg cgctactgca tctcttcttg aaattttacc tagtgtaaat
    12001 tcttctttcg tcagagggtt gcgccagtaa tatgctttgt ctctccttcg atatgttaag
    12061 tttctaggca aattgggatc gtattttttc cgctgcatgt tttaacttct ccattaacgg
    12121 actgtctctc ccttgtcgcc cattaggctg atggtgtgtt atatcggtat caaccttatt
    12181 tgggttgata tagaaagcct ccggaaccac cctgtaactc ctcccgtgta gttcaggtgc
    12241 aggataaatg tttccattcc ttgcccatcg tctcagcgtt gatattgatg gtgggttatc
    12301 cggatatctg agttttcccc acgttttgag tgtcacaaga ttcattgcca tacctctcac
    12361 gatatgaccg ccagtaaata tacagaatac tggcgggtgt ggttgatttt taataa
//
LOCUS       DAECNO010000109        12220 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.109, whole genome shotgun sequence.
ACCESSION   DAECNO010000109 DAECNO010000000
VERSION     DAECNO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.109"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..493)
                     /locus_tag="KKO08_003308"
     CDS             complement(<1..493)
                     /locus_tag="KKO08_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6319439.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KKO08_003309"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="KKO08_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBD6319440.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KKO08_003310"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="KKO08_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBD6319441.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="KKO08_003311"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="KKO08_003311"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBD6319442.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="KKO08_003312"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="KKO08_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBD6319443.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="KKO08_003313"
     CDS             3647..4156
                     /locus_tag="KKO08_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBD6319444.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KKO08_003314"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="KKO08_003314"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBD6319445.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="KKO08_003315"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="KKO08_003315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBD6319446.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="KKO08_003316"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="KKO08_003316"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBD6319447.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="KKO08_003317"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="KKO08_003317"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBD6319448.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="KKO08_003318"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="KKO08_003318"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBD6319449.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYACGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="KKO08_003319"
     CDS             complement(8798..9691)
                     /locus_tag="KKO08_003319"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBD6319450.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="KKO08_003320"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="KKO08_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="KKO08_003321"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="KKO08_003321"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBD6319451.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="KKO08_003322"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="KKO08_003322"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBD6319452.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2969 a         3156 c         3117 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatgcaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 acacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catcgggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgtta attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgcctgcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggagc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtaccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcagaaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAECNO010000110        12153 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.110, whole genome shotgun sequence.
ACCESSION   DAECNO010000110 DAECNO010000000
VERSION     DAECNO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.110"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            126..995
                     /locus_tag="KKO08_003323"
     CDS             126..995
                     /locus_tag="KKO08_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBD6319453.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="KKO08_003324"
     CDS             complement(1198..1551)
                     /locus_tag="KKO08_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBD6319454.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KKO08_003325"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="KKO08_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBD6319455.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="KKO08_003326"
     CDS             complement(3379..3672)
                     /locus_tag="KKO08_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBD6319456.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="KKO08_003327"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="KKO08_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBD6319457.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KKO08_003328"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="KKO08_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBD6319458.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="KKO08_003329"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="KKO08_003329"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBD6319459.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="KKO08_003330"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="KKO08_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBD6319460.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="KKO08_003331"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="KKO08_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBD6319461.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="KKO08_003332"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="KKO08_003332"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBD6319462.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="KKO08_003333"
     CDS             11052..11627
                     /locus_tag="KKO08_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6319463.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="KKO08_003334"
     tRNA            11734..11809
                     /locus_tag="KKO08_003334"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3140 c         2981 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 aaatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caa
//
LOCUS       DAECNO010000111        11923 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.111, whole genome shotgun sequence.
ACCESSION   DAECNO010000111 DAECNO010000000
VERSION     DAECNO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.111"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..2340)
                     /locus_tag="KKO08_003335"
     CDS             complement(<1..2340)
                     /locus_tag="KKO08_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBD6319464.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2274..2897)
                     /locus_tag="KKO08_003336"
     CDS             complement(2274..2897)
                     /locus_tag="KKO08_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBD6319465.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2894..4582)
                     /locus_tag="KKO08_003337"
                     /pseudo
     CDS             complement(2894..4582)
                     /locus_tag="KKO08_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="KKO08_003338"
     CDS             complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="KKO08_003338"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBD6319466.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7505..8227
                     /locus_tag="KKO08_003339"
     CDS             7505..8227
                     /locus_tag="KKO08_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBD6319467.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8355..10286)
                     /gene="yfaL"
                     /locus_tag="KKO08_003340"
     CDS             complement(8355..10286)
                     /gene="yfaL"
                     /locus_tag="KKO08_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBD6319468.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(10299..11894)
                     /locus_tag="KKO08_003341"
     CDS             complement(10299..11894)
                     /locus_tag="KKO08_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HBD6319469.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3307 c         2946 g         2913 t
ORIGIN      
        1 ttcgacattg accagttcac caggctggta atgggttttg tccgttttca cccggatatc
       61 aagctgagga acggcaactt tgatcccggc gttctgaaaa ctgtactgac cattacgggt
      121 atacagcacc gaaaaagtga tgttaggcgc aaaggaattg ctcactggta cccgggcttc
      181 atacagggta tcgtttaaac gttgtagcgt tagccagttt gccggatgcg aaagcagcga
      241 ctgctgttcc acgcgatcgc gttccagcgt caataacgct tcatcaattg gctccgggaa
      301 agtaatcagc atcttcgcgg tttcgcctgg ctggtacagc gttttatccg ccacgatatc
      361 taccgtacca gtatgcgccg tgctgccctt accgctgacg gcatgactta acccagcgag
      421 aattaagccg tctttatcgc gtaatgtcag attgtagttg ccaggtttag cgaaattgac
      481 ggtaaaggat ttgccgcctg acggtagctc tccgctatgg ctcgtgcggt cttcgagacg
      541 caaccattca tacgtaacag gaacctgttt tgaagattcc agcgcggcat aacggaacac
      601 aaccgactcg ccgctattac tgtattgtgc ggcggtactt aaagagtaat gcgccaggcc
      661 gcgttcaatg aggatctctt tggtggtggt gacgcgatac gccgcgccgt cactggcgga
      721 gacggttaac aaatagcggc tcggtttatc ggcggcgggg agatttaacg ccacatgacc
      781 gctggcgtcg gacaccgttt cgctgccttc cagcgacacg gggaaacgtc cggcataacg
      841 caaatcgtta ccgaccattg ataattgctg agcgcgcaaa cttaactgca cgcgggcatt
      901 ttttaccggc tcgccatccg ggtagagcag ttgcagtttg ccgctgaccg cttcgccagt
      961 tttgaactct tttttgtcga gagctaaacc aatctcgaaa tgtggcttga tgtagtttgc
     1021 cacgcgaaaa ctgctgctat agacctgatt gcggtaagca agacgtaact cataacctcc
     1081 ggcaacggca ttttctggca ggcggaaact tccctgcccg ccattgcgcg catccagcgt
     1141 gacatcgacg gtttgcaaca gactgccgtt agcgtccagc accgaaagct tcgccggggc
     1201 gctgacgatg ggggatgaat gcaacggatc gcggaactcg cggcccatca ctttaacatc
     1261 gacacgatcg cctgcgcgat atagcgggcg atcggtaaaa atatacaagc gggtgttgta
     1321 gatttcgctt tcgtagaaga agttctcgga gacaaaaacg ccgccttcag catccttacc
     1381 cagaatgtat gaacgttctg gcgatatatg ttgtaactgc aaagtaccgc tgtcatcggt
     1441 caccccgcgg gtcatcacgc caagaccgtc agtccacaag atctctgagc cgggcttcgc
     1501 ttcaccctgt tttttacccg cggtccatac cagaagctcg ttacctgaca ctttgctaag
     1561 cgccacggta tcggaaacaa acaccaccgt cgtcgcccga tacccaccta ccatcgcctc
     1621 gacgaggtac agccccggct cttgttggcc gagaggaata taaatattac ccggctgcgg
     1681 cgagatgaaa ttgctggatg cgccttccag ttttacccct tgctgcggct cgaccggttt
     1741 agcctgccat agtggataac ggaactgttc caccaaggga tattttttca gcggggaaaa
     1801 ctggttgttc tgtacataac gggaaggttt aataatggca ttgccgagat gtaattcggg
     1861 taatgcctga gtcacattct gccgtgactg agaagagaaa gtacgctgca tcacgcggcg
     1921 agatttgccg taccagttat cccacagcca ggtcagcgta ttgttcagcc cgtcgcccag
     1981 atattgcggt tgcaccacaa tgcgatgcag gtttttctgc tggcgcaaaa atgccatcgg
     2041 gtcaggaata cgatataggc gaacgtccac gccgccgtac tcttccatct gatagcgccg
     2101 ataatcacgc cccggcgctt ccagtcgcac tttcgcctct tcactgctgc taaaactgct
     2161 gtcagcaagc aaaaagaatg ttcccccggc gggcggcgca tagttactgg aaggaagcga
     2221 atcatcagct ttagcaagcc ctgttcccac aagagacagg gacaagcacg ctgctatcgc
     2281 gccagaaaat ttaaacgata aatgccaatg aaattgggat tggaatcgtt gggtatccat
     2341 cgggtgtcct tccatgtcat aagttgttgc agactgactg cgcgcattcc gttgtcagtt
     2401 ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca tccagaccat taagtgctgg
     2461 gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg cctggtttat gtcttggcca
     2521 ataaactggc tgttgtactg aatcaaatta atcgcggtca cgtaggggcc gtttttcccg
     2581 ctcccctgat tccagttttg cgccagttga cgctgttcag gtgttagcgt catctctggc
     2641 ggcaaatact ggctagataa accgttactt tttagccatt tactgtcgtg aactttcagc
     2701 gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct gctgatacca gcgtggactt
     2761 ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc aggcgcgaaa cagcctggat
     2821 tgttcgacat tcagcatttc gctatgggca acaacacaac acagccagca aatcagcgcc
     2881 agcagcccgt gtctcatagc ggctgccagg ttatgggtag ccactgccag gcgacaccgg
     2941 gttccatctg ggccagcttc atgacataac gcggttgttg agataaagcg tccagcttcg
     3001 gcattaataa agtttgtgcg gcgttataaa aaaccggttc gagattcttc ggcagactgg
     3061 tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg attgatatag agcggaacga
     3121 tgccatcagt gggtattaca tccaccattg ccgggcgggt tttattcagt gtttgcaggg
     3181 cgttattaac cagcgtgtca tcgagggaga aaagcagcgt tttgttttgc atcgccagcg
     3241 acacacggaa aaaataatcc gacattaatt gatcgggttg cgccgcctgc gctttcggat
     3301 actgaccgta tcgggaactc acttcgcgac accagatttg cgcttcgccc tgctgagtct
     3361 ggcttaccgg caaaacacct tctggcgctt tgctttcgtg cgcaccaata ttttgcgtaa
     3421 acagtttccc tggcaattgc gcctgttcgg cagtgccatc aaactgaccg acaaacagcg
     3481 gggtttgcaa ttttgagtct tcataccagc acagccccgc ggcaccgtct aacgccccat
     3541 tcagcttgtc gttttcctgg ctgatgtgcg aaagcatctc ttcggcaata ccgtgtgaat
     3601 acggcaccgc cacacagaag ctggctccgg caggcatact gttccagacc ggcgtaaaat
     3661 cgaaactggc atctacgctg gcggattcat catttaacgc cacaaaactg tgccagccgt
     3721 cgttacccat ttcgaagcgt acgccagcaa aagaaggcat taaccgctgg tagccaaaac
     3781 ccagcaacct ggcgctgacc acgatgcgca ggcgtactgg cgttttttcg gctgtacgct
     3841 cttccaggcc aaagctggct tgccagcgtt ttttaccgct caacaaatca cttgcgatcg
     3901 ccgtggcttc ggtatcctgc tgatcatctt taaacaacat atccgtactg gaaaacacca
     3961 gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt gccgttatag cgcaactgat
     4021 aaacaggaat agtttcacta tttatcttga tgctactgat ttccgttttg cttaactagc
     4081 tgtcgctggt agcggcaaac aacaatggct ccagcagttt gcttaaacca ctgcgctgga
     4141 tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg ccatagcgcc gcctgcgcgg
     4201 gctgatctaa gagtgacgaa aagagcttat ctttcagcgt gagatcgtgt tcatagacaa
     4261 tgcggcgaat gctgccttca atgcccagac gatcggcatg attctgataa tagaaaacga
     4321 aatcttcgct cagaacatcg tggagaaacg gaatggtgag gagatctttg ggaagctggc
     4381 tcagagagtc gctgtctaga aagaggtccg gctcattgag atcgatttgc agattgttgt
     4441 gcaccaccag cggcgacaac gttttttctg gcccactgcc agcatattgc aacgcccaga
     4501 cgccagcgga aagcagtgct attgcgccaa aacctacaag accatagaac cgccagcctt
     4561 tcgccttttt ttcaccactc attgccacat tccttgtgta tagccagcca ttttttacgg
     4621 gcacagccaa actttaccgc gccctaatac gacaaaagcc cagactttgc agcctggact
     4681 tttcaattca aacaagggag atagctccct tttggcatga agaagtaaaa ttattcttct
     4741 tctggctcgt cgtcaacgtc cacttccgga gcaatttcat catccccttc cgcggcactg
     4801 ccgtcgatgg tatccagatc ttcctcgtca accggttcag caacacgctg cagacccact
     4861 acgttttcat cttccgcagt acggatgagg atcacgccct gggtgttacg gcccacgatg
     4921 ctgatttctg aaacgcgagt acgtaccagc gtaccggcat cggtgatcat catgatctgg
     4981 tcgcagtcat ctacctgcac cgcgccaacc actaaaccgt tacgttcggt aaccttgatg
     5041 gagataaccc ctttcgtcgc acgcgacttg gttgggtatt ccgccactgc agtacgttta
     5101 ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc cacgaggcac gatcagagag
     5161 acgactttat cgccttcacc taagcgaata ccgcgcacac cagtggtatt gcagcccatc
     5221 gcacggacag aagactcttt aaagcgcacc actttacctt cggcggagaa cagcattact
     5281 tcatcttcgc cactggtcag atcaacgccg atcagctcat cgccgtcaac cagtttgatc
     5341 gccactttac cggcggtacg cagacggttg aattcggtga ggacggtttt cttcacagta
     5401 ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa actcggtcac cggcaggatc
     5461 gcggtgatac gttcgtcctg ttccagcggt agcaggttga cgatcggacg accgcgcgcg
     5521 ccacgagtgg cttccggcaa ctggtaaact ttcatcgaat agacgcgacc acggctggag
     5581 aagcacagaa tatggtcgtg cgtgttcgcc accagcagtc ggtcgataaa gtcttcttct
     5641 ttaatacgtg cggcagattt acctttcccg ccacgacgct gcgcttcgta ttcagaaagc
     5701 ggctgatatt taacgtagcc ctggtgagag agcgtcacga ccacatcttc ctgggtgatc
     5761 agatcttcca ggttgatgtc tgcgctgttg gcggtgattt cagtacgacg tttgtcaccg
     5821 aactgttcac gaaccagctc cagctcttcg cggatcactt ccatcagacg atcggcgcta
     5881 ccaagaatac gcaacagttc cgcgatctga tccagcagct ctttgtattc gtcgagcagt
     5941 ttttcgtgct caaggccggt cagtttctgc aaacgcagat ccagaatcgc ctgagcttgc
     6001 tgttcggtca ggtagtacag accatcacgc acgccgaact caggctccag ccattccgga
     6061 cgcgcagcat cgtcgccagc acgttccaac atggcggcaa cgttgcccag ctgccacgga
     6121 ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg catgacggat cagttcgatg
     6181 atcgggtcaa tgttcgccag cgccacggct aatgcttcaa ggatatgagc acgatcgcga
     6241 gctttacgca gttcgaaaat agtacgacgg gtcaccactt cacggcggtg acgaacaaac
     6301 gccgcgatga tgtctttcag gttcatgatc ttcggctgac catggtgcaa tgccaccatg
     6361 ttgataccga aagaaacctg caactgggtc tgggagtaga ggttgttgag cacaacttca
     6421 ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac cgtctttgtc agactcgtca
     6481 cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca gttccgcaat cttctcgatc
     6541 aggcgcgctt tgtttacctg atacggaatt tcgtggacga taatggtttc acgtccggtt
     6601 ttggcgtcaa cttccacttc agcacgggcg cggatatata ccttgccgcg accggtacgg
     6661 taagcttctt caataccgcg acgaccgtta atgattgccg ccgtcgggaa gtccggcccc
     6721 gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat catcgatata cgccagacaa
     6781 ccgttgatga cttccgtcag gttgtgcggc gggatgttgg ttgccatacc tacggcgata
     6841 ccggaagaac cgttcaccag caggttagga attttggttg gcatgacgtc gggaattttt
     6901 tccgtgccgt catagttatc aacgaaatcg accgtctctt tttcgagatc ggccatcagt
     6961 tcatgggcaa ttttcgccag acggatttcc gtataacgca ttgccgccgc agagtcgccg
     7021 tcgatggaac cgaagttacc ctgaccgtct accagcatgt aacgcagcga gaatggctgc
     7081 gccatacgga cgatcgtgtc ataaaccgcc gagtcaccat ggggatggta tttaccgatt
     7141 acgtcaccaa cgacacgggc agatttttta taggctttgt tccagtcatt gcctagtacg
     7201 ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc catctcggac atctggcagc
     7261 gcacggccaa caatgaccga catcgcataa tccagatagg agctctttag ctcttcctca
     7321 atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct aaccgctatc cctctactgt
     7381 atcccggatt caaaggtcgc aaattataac acagccgcgc agtttgaggt aaacctatac
     7441 gctttattca catccaatgc ctgatatact cgtttgtctt gccaattacg gagtagaagt
     7501 gccaatgaat gccgaaaaat cgccggaaaa ccataacgta gaccacgaag agatcgctaa
     7561 atttgaagcc gtcgcctccc gctggtggga tcttgaaggt gagttcaaac cgttgcaccg
     7621 cattaacccg ctgcgtctgg gctatattgc cgagcgtgct ggcggtttat ttggcaaaaa
     7681 ggtgctcgat gtcggttgtg gcggcggcat tctggccgag agtatggcgc gcgaaggcgc
     7741 gacggtgacc ggtctggata tgggctttga gccattgcag gtggcaaagc tgcacgcgct
     7801 ggaaagcggc attcaggtgg attacgtgca ggaaaccgtg gaagagcacg cggcaaaaca
     7861 tgccgggcag tatgatgtgg tgacctgcat ggagatgctg gagcacgtac ccgatccgca
     7921 gtcagtggtc agagcctgtg cgcaactggt gaaaccaggc ggcgatgtct ttttctcgac
     7981 gcttaaccgc aacggcaagt catggctgat ggcggtggtt ggcgcggaat atattttgcg
     8041 catggtgccc aaaggtacgc atgatgtgaa gaagtttatt aaaccggcag agctgcttgg
     8101 ctgggtggat cagaccagtt taaaagagcg acatataacc gggctacatt acaacccgat
     8161 cactaattcc tttaagctcg gccccggcgt ggatgtgaac tatatgctgc acacgcagaa
     8221 taagtgaggt tgatgtttgg ccgcgccaat gcctgatgcg acgcttgcag cgtcttatca
     8281 ggcctacaaa tgctctccgt aggccagata aggcgtttac gccgcatccg gcaattgtga
     8341 tggtcagaca gcgattacca tttcaccgtc atcgacaaaa accctgccgt ctgggcaaaa
     8401 tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg acactcgctg actgatattg
     8461 cccgtgactc ccacttttat ttcaccccgt agcttcaccg catcgtcact gatagtgctg
     8521 ccatcttctt caatttccgt cgcatgggga tcgtgatagt aattcagatc taatgttggt
     8581 gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca gccgcgtctg aatatcatcc
     8641 ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa aatcatcctg ctgcacgccc
     8701 tgataaatca cctgcgcctg cggttcaatc accacaccac gccccggtaa ccactgatac
     8761 ccgacttcca gcgaggcgat aatccccgac gaatggtaac gatccacgcc atcttcatgt
     8821 tcggaaacat cattgttaaa ccacgcgtat tgcagccagc tatccagcca ggccccctgc
     8881 ttctgattac cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg
     8941 ttatcggcgc gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca
     9001 ccaaccgccc caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg
     9061 ctaagctgca ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga
     9121 taacgtccgc cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt
     9181 cgctccatca taaacgcctg atttgccgcc cgcaggttat tgagataacc gccaactttg
     9241 gcattcagca ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc
     9301 ggcgtgggat caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct
     9361 tgcgatcgca gataccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg
     9421 ttgacgtagc cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca
     9481 gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag
     9541 ttaaccacca cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc
     9601 gagtcatcgc cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg
     9661 tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg
     9721 gtcagatcac cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg
     9781 gtattaagct cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg
     9841 ttaaatagtg aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta
     9901 ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc
     9961 gcgccagtaa cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata
    10021 tccgcaacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg
    10081 ctggcggtca gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga
    10141 tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact
    10201 gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga
    10261 gtatcgacat tggcagtaat ctccatcacg ctttcttgtt acttatcggt aaagataacc
    10321 tggcggttat agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag
    10381 gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc
    10441 gcatcgacca ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca
    10501 cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca
    10561 aggccatcat tgtatgatgt gccgctctgc caagtggaga aatcagagag atcgagcaca
    10621 ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg
    10681 ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac
    10741 tgcgcccctg ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg
    10801 atgatgccag caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa
    10861 gtgccattct gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt
    10921 agctcagcct gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc
    10981 ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta
    11041 acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc
    11101 agatcgccgg aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg
    11161 tcattctctg tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca
    11221 cttaagccga gatacataaa gccacccgcc gcagtggagg gaccatctcc atagcctgct
    11281 gcgctattgt tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa
    11341 atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg
    11401 gctgtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg
    11461 tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat
    11521 gtatatgcct ggttattgtt aaaaacatca tcacttaaat agacgtcatt attaatggta
    11581 taaattgcgc cacctttgcc atcattagcg atattattga tggcgttagt gacacgtaaa
    11641 tcgacggcac cagtatcatt agtaccagaa gaatagattg cgccaccata gccgcctgcg
    11701 acgttaccgg aaaaaataac atcagtaaga ttcagcgttg agttttcttt agcaaatatg
    11761 gccccgccgt tattatattc tcctgtaacg gtgttattgg caaacagagt cattccagtt
    11821 tcattttctg gtaatagtga aaattccgct ccttgttgca aaaatacggc cccaccgctg
    11881 gcattattgg tcatatccga aaaaaccagc cattgcccat cgg
//
LOCUS       DAECNO010000112        11747 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.112, whole genome shotgun sequence.
ACCESSION   DAECNO010000112 DAECNO010000000
VERSION     DAECNO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.112"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            58..852
                     /locus_tag="KKO08_003342"
     CDS             58..852
                     /locus_tag="KKO08_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBD6319470.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            874..2283
                     /locus_tag="KKO08_003343"
     CDS             874..2283
                     /locus_tag="KKO08_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6319471.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="KKO08_003344"
     CDS             2261..3040
                     /locus_tag="KKO08_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBD6319472.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="KKO08_003345"
     CDS             3037..3897
                     /locus_tag="KKO08_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBD6319473.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="KKO08_003346"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="KKO08_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="KKO08_003347"
     CDS             complement(4637..4897)
                     /locus_tag="KKO08_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBD6319474.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="KKO08_003348"
     CDS             complement(4888..6159)
                     /locus_tag="KKO08_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBD6319475.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="KKO08_003349"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="KKO08_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBD6319476.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="KKO08_003350"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="KKO08_003350"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBD6319477.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="KKO08_003351"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="KKO08_003351"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBD6319478.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="KKO08_003352"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="KKO08_003352"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBD6319479.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="KKO08_003353"
     CDS             11501..11653
                     /locus_tag="KKO08_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBD6319480.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2822 a         2990 c         3237 g         2698 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggcgt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggct gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac cagccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacactcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcttggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttta aatttatatt ttttatgtga atatata
//
LOCUS       DAECNO010000113        11667 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.113, whole genome shotgun sequence.
ACCESSION   DAECNO010000113 DAECNO010000000
VERSION     DAECNO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.113"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            597..794
                     /locus_tag="KKO08_003354"
     CDS             597..794
                     /locus_tag="KKO08_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319481.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="KKO08_003355"
     CDS             complement(744..1937)
                     /locus_tag="KKO08_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6319482.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="KKO08_003356"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="KKO08_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBD6319483.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="KKO08_003357"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="KKO08_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBD6319484.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="KKO08_003358"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="KKO08_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBD6319485.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="KKO08_003359"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="KKO08_003359"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBD6319486.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="KKO08_003360"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="KKO08_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBD6319487.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="KKO08_003361"
     CDS             complement(10573..10716)
                     /locus_tag="KKO08_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319488.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10966..11667
                     /locus_tag="KKO08_003362"
                     /pseudo
     CDS             10966..11667
                     /locus_tag="KKO08_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2960 a         2899 c         2993 g         2815 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta
    11581 ttaatggtga atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta
    11641 gtctatttga ggtcacccaa tgggact
//
LOCUS       DAECNO010000114        11400 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.114, whole genome shotgun sequence.
ACCESSION   DAECNO010000114 DAECNO010000000
VERSION     DAECNO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.114"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..114
                     /locus_tag="KKO08_003363"
     CDS             <1..114
                     /locus_tag="KKO08_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6319489.1"
                     /translation="GPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            111..512
                     /locus_tag="KKO08_003364"
     CDS             111..512
                     /locus_tag="KKO08_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6319490.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            523..1263
                     /locus_tag="KKO08_003365"
     CDS             523..1263
                     /locus_tag="KKO08_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBD6319491.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1322..1708
                     /locus_tag="KKO08_003366"
     CDS             1322..1708
                     /locus_tag="KKO08_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBD6319492.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1717..2046
                     /locus_tag="KKO08_003367"
     CDS             1717..2046
                     /locus_tag="KKO08_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBD6319493.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2018..4756
                     /locus_tag="KKO08_003368"
     CDS             2018..4756
                     /locus_tag="KKO08_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD6319494.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4756..5085
                     /locus_tag="KKO08_003369"
     CDS             4756..5085
                     /locus_tag="KKO08_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6319495.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5085..5783
                     /locus_tag="KKO08_003370"
     CDS             5085..5783
                     /locus_tag="KKO08_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBD6319496.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5788..6531
                     /locus_tag="KKO08_003371"
     CDS             5788..6531
                     /locus_tag="KKO08_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6319497.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6468..7070
                     /locus_tag="KKO08_003372"
     CDS             6468..7070
                     /locus_tag="KKO08_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBD6319498.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7131..10611
                     /locus_tag="KKO08_003373"
                     /pseudo
     CDS             7131..10611
                     /locus_tag="KKO08_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            10679..11278
                     /locus_tag="KKO08_003374"
     CDS             10679..11278
                     /locus_tag="KKO08_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBD6319499.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT         2676 a         2694 c         3641 g         2389 t
ORIGIN      
        1 ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg
       61 aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa atgaaccgac
      121 acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga gacagcgcca
      181 cgttttttga cgggcttccg gcatttattg atgcgcagga actgcctgcc gtggcggtgt
      241 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
      301 tgcatattgc cgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
      361 agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc atcgacaccc
      421 tgaacccact cggttttaac tatcaacgtg ataatgagat ggctacctgg gcgatggcgg
      481 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
      541 ctggcaaaga cgaaaggtgc ggggacgacg ttctggatgt ataccggcaa cggcgatgcg
      601 tttgcgaacc ctttgtcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
      661 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
      721 tggaaaacga caacccaggg gcagaaatcc gttggtgata cttcggcgac gctggcctgg
      781 cgtccgggtg acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc
      841 gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgcggctg gctgagctca
      901 ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat cagcggtgtg
      961 gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gtgttaccgg actgacggtg
     1021 gcaccggcat ctgtcagtgt caaagcggga gcaaccacca cactgacctt tacagtaaaa
     1081 cctgacgggg ccagtgacaa agcgatcagt gtgcattcgt cagatcctca gactgcttcg
     1141 gtgaccctga gcgggcttgt ggccacggtg aaaggcgtga agcagggcag tgtcagcatc
     1201 gtgggcatga cctctgacgg agagtttgtg gcagtggctg cggtgaccgt cagcgcacca
     1261 taacaggacg atactcatca ttgccccggt tatccggggc tttttgcatc cggagaacat
     1321 gatgtttctg aaacaggata cgtttaatta tgaaaaacag tccgtggtgc tcagtgagct
     1381 gtccgggctg cagagaattg aatatctgac gtttgttcag cagcgaacgg caaagtttga
     1441 tgcacaggag ggagaactgc cggaggctga acgacagatt gcttttctgc gtatgggaat
     1501 ggatatcaat gcctggctgg tttcccgctc actgtggaat gctgagcagt ctcaggatgt
     1561 tgagacgctt tgcgcatcca ttatgacaac atggtcgtat gatgcgctgg gcgcgggggc
     1621 ggagagggtt ctgtcgctga gcggtatggg gaccattgag aatgccgggg atgatgatca
     1681 tgaggcgctg acgccggaaa agtcctgacg cgggaaatgc agtttgtcat gcggcttgcc
     1741 cgggagttcc ggcgggcaga ctggcggcgg atgctgtcgg atatgtcggc cactgagctt
     1801 ggtgagtggg gcgattattt ccggatgcag agcttcagtg atgtgtggat ggatgcgcag
     1861 tttgcctcgc tgaaggcatt gatcgtgaga atggtgtccg gcagcagcga tgctgcggtg
     1921 gctgatttca gccttttacc ggaagagaac gggataccgg agcgaacgga cgaagaactg
     1981 atgcatcttg gggaaggtat ttccggaggt gtgcgttatg gaccagatag ccaacctggt
     2041 cattgatttg gggattgatg cggcagagtt taaaaatgaa attccccgta tcaaaaacct
     2101 tctgaatggt gcagccagcg atgcagaacg gtcttctgcc cgtatgcagc gttttatgga
     2161 gcgtcagact caggcggccc ggcagacaat gcaggcggct tcttcggctg caacagcagc
     2221 atcagcccat gcgcagacgg tggagaagaa tgcacgggct catgaacgca tggcccgtga
     2281 ggtggaacaa acccgtctgc gcgtggatgc cctgaatcag aaaatgcgcg aggaacaggc
     2341 gcaggccagg gcggctcagc tgggggtaag cagtgctgca gaagtgtata tccgcaaaat
     2401 ggagcgggca ggaaaagcca cacattcgct ggggctgaaa agtgcggcag cccggcggga
     2461 gctgggggtg ttaatcagtc agatggcgcg cggcaatttt ggtgcgctga ggggatccgg
     2521 gataacgctg gctaaccgtg ccggatggac aggcgcactg atgtcgccga aaggcatgat
     2581 gactggcggc gttattggcg gacttgtcgc ggcggtcctg ggtctgggta aagcctggca
     2641 tgacggccgg aaggagggcg agaaatttaa ccgtcagctg gcgctgacgg gacattatgc
     2701 cggtgtcact gtcgggcagt tgtggaaact cagccgggcc atatccggga atggtatcac
     2761 gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc agtggggcat ttcatggaaa
     2821 cgatattggt atggtggcga aagctgccgc acagatggag cgatcggttg gccagtcggt
     2881 cagcgatacc ataaatcagt ttaagcggct gaaggatgat cctgtaaatg ccgcgaaggc
     2941 gctggacaat gcgctgcatt ttttgaatgc cactcagctt gagcagatac gcgtccttgg
     3001 ggagcagggg cggtccagtg atgcggcccg gatcgccatg tctgcgctgg cagaggaaac
     3061 cggtaaacgc acatctgata ttgataataa tctcaatgcg ctgggtagta cgctgcaaac
     3121 cttgtctgac tggtggaagc agttctggga tgcggccatg aatattggtc gtgaagactc
     3181 gctggatgcg cagattgatg cgttacagga aaaaattcag cgcgcgaaaa aatatccgtg
     3241 gacaaacgcc tccacacagg tggagtacga tcagcagcgt cttaacgatc ttcaggagaa
     3301 aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca gaacggaatt accaggagca
     3361 acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg atgaatgaaa cggaagctgc
     3421 acgacatcag cgggaaattg cgcgtattaa tgccatgcag tacgccgacc aggctgtcag
     3481 ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa gccattaaga aaaatacacg
     3541 ggcaacccgt aatgatgagg ccacccggtt attgctgcag tacagtcagc agcaggcaca
     3601 ggtggaaggg cagattgctg ctgccagaca gtcagcaggc attgccactg aaaggatgac
     3661 tgaagcgcat aaacagcttc tggccctgca gcagcgcatc agcgacctgg acgggaaaaa
     3721 gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat gagctgattc aggcgctgac
     3781 gctgctggat gtgaaacagc aggagctgca gaaacagaca gcgcttaacg acctgagaaa
     3841 aaaaacggtt cagctgacca gccagctggc agacaaagaa cgtgcactgc gtgagcagca
     3901 caatctggat attgccactg caggtatggg ggataagcag cggcagcgct accaggcaca
     3961 gttgcgcatc cggcaggaat accggcaaca gttgcaacag cttgagaatg acagtcgcca
     4021 gaaaggcact tacgggacgg aggactaccg gagggctgag gaggtgctga aggggagcct
     4081 gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa ctggaagtgg cgcagggcga
     4141 ctggaaaaac ggtgccatgc gggcgtttca gaattttacg gcggatgcgg ataatgcggc
     4201 gggaacggca gaacagatgt ttacagtggc attcagcagt gccggtaatg cactggcgac
     4261 attctgtacc accggtaagc tgaattttaa atccttcacc tcttccctgt tgtcagatat
     4321 ggccagaatt atggcacaga tggccatgat gcaggcggta aagggcgtcg gttctttatt
     4381 cggcttcacg actaatgctg atggcggtgt ttaccagtct gctgatttga gtcgctacag
     4441 tggcacggtg gttaaccgtc cgacgttttt tgcttttgca aaaggcgcgg gtgtgatggg
     4501 ggaagcggga cctgaagcca ttctgccact gcgtcgcggt gctgatggta agctgggggt
     4561 tgtggcggat actggtggtt caggcatggt gatgtttgcc ccgcagtaca acattgagat
     4621 caataacgac ggcacgaacg ggcagatagg tccggctgcc ctgaaggtgg tttatgacct
     4681 cgggaaaaaa gcggccgcgg actttatgca acagcaggcc cgtgatggtg gccggttaag
     4741 tggagcatac cggtaatgga gacgtttcac tggaaagtgc gcccggatat gaatgtggta
     4801 tcagagccga aagtggtgac agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg
     4861 ggactgaata accagttgtc gacttacagc gtgacgatac gtgttcgtaa aggtgaacac
     4921 ccatctttaa aagcctttct ggaacggcac ggtggcgtcc gcgcatttca gtggacgcca
     4981 ccttatgact ggaaactgat cagggtggtt tgtcgtaaat ggtcggcaag cgtgggggcg
     5041 ttatgggtga ctgtaacggc cgattttgaa caggtggtga actgatgcag gatatccggc
     5101 aggaaacact gaatgaatgc acccgtgcgg agcagtcagc ccgggtggag ctctgggaaa
     5161 tcgatctgac agaggtcggt ggtgagcgtt attttttctg taatgagcag aacgaaaaag
     5221 gtgagccggt cacctggcag gggcggcagt atcaggcata ccccattcag gggtcggggt
     5281 ttgaactgaa cggcaggggc tgtgctgccc gtccgacact gacggtctct aacctgcacg
     5341 gcatggtcac cgggatggcg gaagatctgc agagtctggt cggcggaacg gtggtccggc
     5401 gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agcgacgccg
     5461 atccggaaca ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa ctgagcgcgg
     5521 tcagtgcctc ttttgtgctg gccacaccaa cggagacgga tggcgcggtt ttcccggggc
     5581 gtatcatgct ggcgaatacc tgtatgtgga cttaccgttc tgatgagtgt ggttacacgg
     5641 gcagggcagt ggctgacgag ttcgacaaac caacaacgga tatccggaag gacaaatgca
     5701 gcaagtgtat gcgcgggtgt gagttgcgca acaataccgg taatttcggc ggtttccttt
     5761 ccatcaataa actttctcag taaatccatg acacagacag aatcagcgat tctggcgcac
     5821 gcccggcgat gtgcgccagc ggagtcgtgt ggcttcgtgg tgagaacgcc ggagggagac
     5881 aggtatcttc ccagcgagaa tatctccggt gagccggagg aacggttccg gatggctccg
     5941 gaggactggc tgcgggcaca aatgcagggt gagattgtgg cactggtcca cagtcatccc
     6001 ggtggtctgc cctggctgag tgaggctgac cggcggctgc aggtgcagag tgatttgccg
     6061 tggtggctgg tctgccgggg ggcgattcac aagttccgct gtgtgccgca tctcaccggg
     6121 cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc ttatcatctg
     6181 gcggggattg agatgccgga ttttcatcgc ggggatgact ggtggcgtca cggtcagaat
     6241 ctctatctgg ataatctgga ggccacaggg ctgtatcagg tgccgttgtc atcagcacaa
     6301 ccgggcgatg tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac
     6361 tgcggcgatg gtgagctgct gcaccatatt cctgaacaac tgagtaaacg agagaggtat
     6421 accgacaaat ggcagcgacg cacacactcc ctctggcgtc accgggaatg gcacgcatct
     6481 gcctttacgg ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacggggg
     6541 ctgaagccat ccgcgcactg gccacacagc tcccggcgtt tcgtcagaaa ctgagtgacg
     6601 gctggtatca ggtgcgcatt gccgggcgtg atgcaggtga aaccgaattg tctgcccgtc
     6661 ttaatgagcc gctggcaaat ggtgcagtga tccacatagt accgcgtctg gtgggagcta
     6721 aaagtggcgg tgtgtttcag gcggtgctgg gggcagctgt tatggcggtt gctatatgga
     6781 tgccgggggt aggaattatg gcgagtaatc tgctgttttc tctcggtgcc agtatgacgc
     6841 ttggcggtgt tgcacagatg ctggcaccga aagccagaac tccccgtaca cagacaacgg
     6901 ataacggcaa acagaacacc tgtttctcct cactggataa catggttgcc cagggcaatg
     6961 ttctgcctgt tctgtacggt gaaatgcgcg tggggtcgcg ggtggtatct caggagatca
     7021 gcacggcaga cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt
     7081 ttcatgtgaa accgcctgcg ggcggttttg tcgtttatgg agcgtgagga atgggtaaag
     7141 gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa cctgaaatcc acgcagttgc
     7201 tgagtgtgat tgatgccatc agcgaagggc cggttgacgg tccggtggat ggattaaaaa
     7261 gcgtgctgct gaacggtacg ccggtcctgg atagcgaggg gaagacaaac ttttccggtg
     7321 ttacggtggt gttccgcgcc ggcgagcagg agcagacacc gccggagggg tttgaatctt
     7381 ccggctcaga gactgtgctg ggtacggaag tgaaatacga cacgccgatc acccggacca
     7441 tcacgtcggc aaacattgat cgtctgcgcc tgaccttcgg tgtgcaggca ctggtggaaa
     7501 ccacctcaaa gggggaccgg aatccgtcgg aagtccgcct gctggttcag atacagcgta
     7561 acggtggctg ggtgacggaa aaagacatca ccattaaggg caaaaccact tcgcagtatc
     7621 tggcctcggt ggtggtgggt aacctgccgc cgcgcccgtt taatatccgg atgcgcagga
     7681 tgacaccgga cagcaccaca gaccagctgc agaacaaaac gctctggtcg tcgtacaccg
     7741 aaatcatcga tgtgaaacag tgctacccga acacggcgct ggtcggcgtg caggtggatt
     7801 cagagcagtt cggtaaccag caggtgagtc gcaattatca tcttcgcggg cgcattctgc
     7861 aggtgccgtc gaactataac ccgcagacgc ggcaatacag cggtatctgg gacggaacgc
     7921 ttaagccagc atacagcaac aacatggcct ggtgtctgtg ggacatgctc actcatccgc
     7981 gctacggcat ggggaaacgt cttggtgcgg cagatgtgga caaatgggcg ctgtatgtca
     8041 tcggccagta ctgcgaccag tcagtgccgg acggatttgg cggcacggag ccgcgcatca
     8101 cctgtaacgc ttacctgacc acacagcgta aggcgtggga tgttctcagt gatttctgct
     8161 cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct gacgttcgtg caggaccgac
     8221 cgtcggataa agtgtggacc tataaccgca gtaatgtggt gatgccggat gatggcgcgc
     8281 cgttccgcta cagcttcagc gccctgaagg accgccataa tgtcgttgag gtgaactgga
     8341 ttgatccgga caacggtcat gagacggcga cagagcttgt ggaggacacg caggccattg
     8401 tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg ctgtaccagc cggggacagg
     8461 cgcaccgcgc cgggctgtgg ctgattaaaa cggaactgct ggaaacgcag accgtggact
     8521 tcagcgtcgg cgcagaaggg cttcgccatg taccgggtga tgtcattgaa atctgcgatg
     8581 atgattatgc cggtatcagc accggtggtc gcgtactggc ggtgaacagc cagacccgga
     8641 cgctgacgct cgaccgtgaa atcacgctgc cgtcctccgg taccacgctg ataagcctgg
     8701 ttgacggaaa tggcaatccg gtcagcgtgg aggttcagtc cgtcaccgac ggcgtgaagg
     8761 tgaaagtgag ccgggttcct gacggcgttg cagaatacag cgtgtggggg ctgaagctgc
     8821 cgacgctgcg ccagcgcctg ttccgctgcg tgagtatccg tgagaacgac gacggcacgt
     8881 atgccatcac cgccgtgcag catgtgccgg aaaaagaggc catcgtggat aacggggcac
     8941 actttgacgg tgaccggcgc ggcacggtga atggtgtcac gccgccagcg gtgcagcatc
     9001 tgaccgccga agtcaccgca gacagcgggg aatatcaggt gctggcgcga tgggacacgc
     9061 cgaaggtggt gaagggtgtg agcttcctgc ttcgcctgac cgtggcagcg gacgacggca
     9121 gtgagcggct ggtcagcaca gccaggacga cggaaaccac ataccgcttc aggcagctgg
     9181 cgctggggaa ttacagtctg acagtccggg cggtaaatgc ccgggggcag cagggcgatc
     9241 cggcgtcggt atcgttccgg attgcggcac cggcagcgcc tgtcactatt gaactgatac
     9301 cggggtattt tcagataacg gtggtcccga aactggctgt atatgacccg acggtgcagt
     9361 ttgagttctg gttttcggaa aagcggattg cggatatcag gcaggttgaa accagcgccc
     9421 gctatcttgg cacggcgctg tactggatag ccgccagtat caatatcagg ccgggccatg
     9481 attattattt ttacgttcgc agtgtgaaca ccgttggcaa atcggcattt gtggaggccg
     9541 tcggtcgggc gagcgatgat gcggaaggtt atctgagttt ttataaaggg ttgatcaata
     9601 aaacgcatct cggcaaggag ttgtggacgc agattgataa cggtcagctt gcgccggacc
     9661 tgactgaaat caggacgtcc attacgaatg tcagcaatga aatcacgcaa accgtcaata
     9721 aaaaactgga aaatcagagt gctgcaatcc agcagataca gaaagttcag gttgatacaa
     9781 ataataacct gaacagcatg tgggctgtga agctgcaaca gatgaaggac ggacgccttt
     9841 atattgcgtg gtatcggtgc cggtattgag aatacgccag caggaatgca gagtcaggtg
     9901 ctgctggcgg cagacaggat tgcgatgatt aatcctgcga atggcaacac aaagccgatg
     9961 tttgttggtc agggcgatca gatattcatg aacgacgtgt tcctgaaacg cctgacggct
    10021 ccgaccatta ccagcggcgg taatcctccg gcattttccc tgacacctgg cggacggctg
    10081 acggcgaaaa atgccgatat cagcggtaac gtgaacgcga actccgggac gctcaacaac
    10141 gtcacgatta ataagaactg tcgggttctg ggaaaattgt ccgcgaacca gattgaaggc
    10201 gatctcgtta aaacagtggg caaacctttc ccacgggact cccgggcacc ggagaggtgg
    10261 ccatcaggga ccattaccgt cagggtttat gacgatcagc cgtttgatcg gcaaattgtt
    10321 attcccgcgg tggcgtttcg cggtgctaaa catgagcgga agaataacaa tatttattcg
    10381 tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt ataaccgaac gaccctggat
    10441 aatacgctga tatatacggg tgttattgat atgcctgccg gtcacggtca catgacgctg
    10501 gagttttctg tatcggcatg gctggtaaat ggctggtatc ccacagcaag tatcagcgat
    10561 ttgctggttg ttgtgatgaa gaaatccaca gcaggtatca gtatcagctg aattttataa
    10621 cccagatacg ggcaccagaa atggtgcctt ttttattgca gaaaagcgag aggtaattat
    10681 gcgtaaactt tatgctgcca ttttgtccgc agccatctgt ctggctgtat ccggtgcgcc
    10741 tgcatgggcg tctgaacatc agtccacgct gagcgcgggg tatcttcatg cctcgacgaa
    10801 cgctcccggc agtgatgatc tgaacgggat taacgtgaaa taccgttatg agtttacgga
    10861 cacgctgggg ctggtgacgt cattcagcta tgccaacgct gaagatgagc aaaaaacgca
    10921 ttacagtgat acccgctggc atgaggattc cgtgcgtaac cgctggttca gcgtgatggc
    10981 ggggctgtct gtgcgcgtga atgaatggtt cagcgcgtat gcgatggcgg gcgtggctta
    11041 cagccgtgtg tcgaccttct ccggggatta tctccgcgta actgacaaca aggggaaaac
    11101 gcacgatgtg ctgaccggga gtgatgacaa ccgccacagc aacacgtctc tggcgtgggg
    11161 ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt gaccttgctt atgaaggttc
    11221 cggcagtggc gactggcgca ctgacggttt catcgtgggt gtcggttata agttctgatt
    11281 agccaggtaa cacagtgtta tgacagcccg ctggttcagg cgggcttttt gtggggtgaa
    11341 tatggcagta aagatttcag gtgtactgaa agatggggcg ggtaaacctg tcgtaaattg
//
LOCUS       DAECNO010000115        11303 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.115, whole genome shotgun sequence.
ACCESSION   DAECNO010000115 DAECNO010000000
VERSION     DAECNO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.115"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KKO08_003375"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="KKO08_003375"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBD6319500.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVEPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KKO08_003376"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="KKO08_003376"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBD6319501.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KKO08_003377"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="KKO08_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBD6319502.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="KKO08_003378"
     CDS             6038..6658
                     /locus_tag="KKO08_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBD6319503.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="KKO08_003379"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="KKO08_003379"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBD6319504.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KKO08_003380"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="KKO08_003380"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBD6319505.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KKO08_003381"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="KKO08_003381"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBD6319506.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="KKO08_003382"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="KKO08_003382"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBD6319507.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KKO08_003383"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="KKO08_003383"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2653 a         3251 c         2781 g         2618 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggctc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccactg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccattgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaagg
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac agg
//
LOCUS       DAECNO010000116        11014 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.116, whole genome shotgun sequence.
ACCESSION   DAECNO010000116 DAECNO010000000
VERSION     DAECNO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.116"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            156..506
                     /locus_tag="KKO08_003384"
     CDS             156..506
                     /locus_tag="KKO08_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBD6319508.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            559..954
                     /locus_tag="KKO08_003385"
     CDS             559..954
                     /locus_tag="KKO08_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBD6319509.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            995..1738
                     /gene="cmoA"
                     /locus_tag="KKO08_003386"
     CDS             995..1738
                     /gene="cmoA"
                     /locus_tag="KKO08_003386"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBD6319510.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1735..2706
                     /gene="cmoB"
                     /locus_tag="KKO08_003387"
     CDS             1735..2706
                     /gene="cmoB"
                     /locus_tag="KKO08_003387"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBD6319511.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="KKO08_003388"
     CDS             complement(2871..5300)
                     /gene="torZ"
                     /locus_tag="KKO08_003388"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBD6319512.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5325..6425)
                     /locus_tag="KKO08_003389"
     CDS             complement(5325..6425)
                     /locus_tag="KKO08_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBD6319513.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6813..7559)
                     /gene="cutC"
                     /locus_tag="KKO08_003390"
     CDS             complement(6813..7559)
                     /gene="cutC"
                     /locus_tag="KKO08_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBD6319514.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7573..8139)
                     /locus_tag="KKO08_003391"
     CDS             complement(7573..8139)
                     /locus_tag="KKO08_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBD6319515.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8355..10088
                     /gene="argS"
                     /locus_tag="KKO08_003392"
     CDS             8355..10088
                     /gene="argS"
                     /locus_tag="KKO08_003392"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBD6319516.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10265..10753
                     /locus_tag="KKO08_003393"
     CDS             10265..10753
                     /locus_tag="KKO08_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBD6319517.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10871..10939)
                     /locus_tag="KKO08_003394"
                     /pseudo
     CDS             complement(10871..10939)
                     /locus_tag="KKO08_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         2689 a         2811 c         2628 g         2886 t
ORIGIN      
        1 cataatgggc ttttaatcct atcgtttatt gcttgcatag tagaatctaa ccatccatca
       61 ctcatagcta accctcggaa atcttacatt tctgtctatc ataaccacta gcatcacctt
      121 taggaaccat ctgaaacggg aggtgtattt agaccttgct aatcttagac agccgcccgg
      181 ttcatgcatc acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa aaacctaaag
      241 ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc ggtagtgatg
      301 atatgacgca aaaccgggaa ttatttcagg tctggttaca aaaattagcg cagtggcatc
      361 agaccactac gccttatctt tttttacata cgccagatat tgcccaggcc ccggaactgg
      421 tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca gttccggcta
      481 ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt gccatcggcc
      541 attttaaagg gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc gttgttatta
      601 atgaagttct cttttgatgt cgttcgcctg cgaatgcagt accgcgttgc ctatggtgac
      661 ggtggtttta gcgaactgca aagcgctatt cgcattcatg gtaacgcggt ggaatatatt
      721 cccatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac ctggatggtg
      781 catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg ttttcatcac
      841 cgtctgtttc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct gttgctgatg
      901 gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg ttagcgcaca
      961 atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca cgctattttc
     1021 tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtcgctg aagtcttccc
     1081 ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga ttggtatgtt
     1141 agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt ctctgggcgc
     1201 ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta ttgccatcga
     1261 caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata aagcccctac
     1321 gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg catcgatggt
     1381 ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat tactggataa
     1441 aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat tcagtttcga
     1501 agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac gtgccaacgg
     1561 ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga tgctgaccga
     1621 ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata gcgagctgtg
     1681 gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg ctgcatgatc
     1741 gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg gctcgaaacg
     1801 ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt taagcagtgg
     1861 tccaacacgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt attgcatagc
     1921 gtaaccgccg aaagcgaaga gccactgagc accgggcaaa ttaaacgcat tgaaacgctt
     1981 atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt caacatcgat
     2041 accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc tgatttaacc
     2101 ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg catgattggc
     2161 gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg ccagtttgaa
     2221 gcggtgcgta aactactggg taacgatcag cgcgcacatt tgttaccgtt aggtattgaa
     2281 cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct ttaccatcgc
     2341 cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga gggtgaactg
     2401 gtgctggaaa cgctggttat tgatggcgat gagaacacgg tgctggtacc aggcgatcgt
     2461 tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa aaactggctg
     2521 aagaagtgtg gttttgtaga tatccgcatt gtggatgtgt gcgttaccac tactgaagag
     2581 cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga cccacatgat
     2641 ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat tgcgcgcaag
     2701 ccgtaaaggt ctggtattac tgccggatgc ggcgtgaacg ccttatccgg cctacaaacc
     2761 cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc gcatcaggca
     2821 gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc ttacgcctga
     2881 acagcaggct tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt ttcgatatac
     2941 accagcgcag agttaccggc acaggcattt gcccgctgcg agctggggat atccgccgtt
     3001 aacacgttcg cactgccgtt tttacacaag ccgttttcca gatctggcca tgcaccttca
     3061 tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt caaaatctga
     3121 ccacgtttgt tccagacacg caccagatcg ccattcgcaa taccaaatcg agcagcatct
     3181 tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg cagttccgca
     3241 tagttaagct gactgtgtaa acggtgtgcc ggatgtgcgg tcagaagctg caactgcttc
     3301 tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc cgggcaatcc
     3361 ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct tggcgtaccc
     3421 agcgcatttt tcaccggatc ggcgcggaaa tcaccataac gaacgtactg ttcgttcttc
     3481 tcgctgcggc gcatttcgat cagtttattt tgctgccaga aggcattaaa cattggcata
     3541 gtgacgcgtt gcgcacgggc ccctttctga gcggcatcat agaaaaattt cagccacgcc
     3601 atttcatctt taccttcggt atagatctct tttccgccag gtttgagtaa ttccgcaaga
     3661 tcggcaaaca cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc ctgcttcatc
     3721 ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg ctcaaacgat
     3781 gtggtgatcg gtaatacgat atctgcgtgt ttggctgctg cggtccagta gcattcagaa
     3841 acgacgatca tctccggttt ctgccaggct ttgatcagac gattagtatc ctggtgatgg
     3901 gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt ttgttcttta
     3961 ccgttatgct gatatttgcc gccaggattt tccagcgcat cgacgatacg ggcaacagga
     4021 atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat cgccgcagac
     4081 atttccggca gcacgccgcc aacacgcgtc gggttgccac cattggagta atgataagag
     4141 aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag cgtcaccagc
     4201 atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagcctgc catcagcatt
     4261 gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta
     4321 atttccgccg tccagaccgc gctcttcggc gtattatcgc tcttacctgt cagatactct
     4381 tcaaactgcg gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt
     4441 gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg
     4501 atccaggtgg cattatcgcc aaagaattcg atggtttcgg agcggatagg atcaatggca
     4561 atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta
     4621 ctgctccagg caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg
     4681 ctgttttcca gaatcagcgg ccaggaggcc tgctgttcat acacctctac agaaccgacc
     4741 acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa
     4801 ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa
     4861 gagcgccagc cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc
     4921 tcatgttgct catgaatcag ttttaatgcc tgttcccagc tcacctgtac ataggtatct
     4981 tcgccacgac ctttcgccgg ttgcagtgga ttgtcgagat agctttttct caccatcgga
     5041 tgctgaatac gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt
     5101 atggttttcg ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgctc
     5161 atcgctcccc aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggg
     5221 agcggtgctg ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat
     5281 tcacgtcttg ttaatgccat aacttcctcc ctgatcaacg aggatcactg tttctcggta
     5341 atatctttgg cgttgtactg gaaataccgc gttaaaatgt ccggttcgtt ttcgctcatg
     5401 ctggttcgtg cccccattcc tttggcaatg gacggccacg cattgacggt gtaatggtcg
     5461 gcggcaatag aggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc
     5521 catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga
     5581 cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata
     5641 ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta gagcgtgttt
     5701 tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca
     5761 acgacggtga gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg
     5821 aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc
     5881 gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg
     5941 atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc
     6001 gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct
     6061 tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa cttatcgtca
     6121 ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagca
     6181 aataaataat ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct
     6241 ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg
     6301 caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaataac
     6361 ccgccgccac caaccacaac cgctatcagc aaaaacaata acccaatgcg ttttttccct
     6421 cgtatagatt cctctaaata aattccagaa caccgaaatc attgtataat taaatacaca
     6481 acgaattaac cgcttgcata actcggcaca acactgcctg aaataatcga taaagaatat
     6541 gatttattac aatgtaatca ttaattgcta aggaataacc cagttgctat ttagaatgta
     6601 tatttatcag ttttagcaat ttaaatccca taatcaatgt gaatataaaa aatattttta
     6661 gcacaacgct atgcgcactg tcacccgctc aacagatcac ggttatcgtt cgttttttat
     6721 actgctcagg gataaaaaaa ggcccctgtt gaaattgcag gggcctggta cgagcaagca
     6781 tcatattggg cgacatgatg caacggtaaa aatcatttgg cctgatggcg ttcaatgatt
     6841 cctttcattt cagcaaccgc cgccccgtct acgatataac gcgaatactc gtccgcgtgt
     6901 tcatctgatg acatggacaa tccttgatta cgataacgca tcggtgaggc ttgccacgct
     6961 cccgcggagc tatggacttc cagcactccg gcatcgagga agtggtgcag gttttctgca
     7021 cggactcctg ctccagccat aatgattgga gcatcacgat gggcaataag ttccataatt
     7081 tttgataaac cttgcagtgc gtctgatttt tgccctgatg tcagtacccg ggcaatgccc
     7141 aattccgcaa gattattgag tgtatataaa gggttagcgc acatatcgaa ggcgcgatga
     7201 aaagtcactg ccagcggacc ggcagcagcc attatttttt ccattcgtgg catatcgaca
     7261 ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa aacctaattc gcggaccgtg
     7321 cgcacatcct caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc accaccgcgt
     7381 gggcgaatta tcggatgcac agggatcgtc acccgctggc gcacggattt cagtaccccc
     7441 aacgacggcg ttaagccccc ctcttttggg gctgcgcata attcaactct gtctgcgccg
     7501 ttttgctgcg ccgttagtgc acattccatg ctgtaacagc aaatttccag taatgccatt
     7561 tttgctcctt aattacgctg actgctcgct ggcaacgatc tcttcaatgg accacggatg
     7621 aaacttaata gttgttttgc catcggtgac cgccagcgtc ggattcggta atcgctcatg
     7681 ctcacctttt ggtgaactgg ttttaactga gatccccggt aggctgagtc cttcatcaga
     7741 aagcaatgcc agcgcacggg cattcagcgt ttctggatca cccggcagaa cgatttcaat
     7801 atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc cacggtagct ccacattgga
     7861 aaactgccag tgcgcaacct gtaccggttc atgcaattta aacagacaaa tcggtctgcc
     7921 attgatcatt ttttctgaca aaagctcgcc acactgttca aaaccgcgac gccagcgctc
     7981 agcggtgacg ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg gagtgatatc
     8041 caaccccaga cggcgggaaa gttcatctaa tgcgtgggta aatcgcggta aatccgatgc
     8101 aatatcctgc agctcgtcga tagattgcca gttcgccata atcactcttc gtctttcagt
     8161 aaaagcgtta atttaccctg ttgccctgtg ccaaccaacc gctgatttca cgccgcttct
     8221 gatgcaatag tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa cagccatttg
     8281 cagtatactc ccgccctaat ttctttaact ggtgcgggca atttttgctc gcttcatcaa
     8341 tgtaaggtat tccggtgaat attcaggctc ttctctcaga aaaagtccgt caggccatga
     8401 ttgcggcagg cgcgcctgcg gattgcgaac cgcagattcg tcagtcagca aaagttcagt
     8461 tcggcgacta tcaggctaac ggcatgatgg cagttgctaa aaaactgggt atggcaccgc
     8521 gacaattagc agagcaggtg ctgactcatc tggatcttaa cggtatcgcc agcaaagttg
     8581 agatcgccgg tccaggcttt atcaacattt tccttgatcc ggcattcctg gctgaacatg
     8641 ttcagcaggc gctggcgtcc gatcgtctcg gtgttgctac gccagaaaaa cagaccattg
     8701 tggttgacta ctctgcgcca aacgtggcga aagagatgca cgtcggtcac ctgcgctcta
     8761 ccattattgg tgacacagca gtgcgtactc tggagttcct cggtcacaaa gtgattcgcg
     8821 caaaccacgt cggcgactgg ggcactcagt tcggtatgct gattgcatgg ctggaaaagc
     8881 agcagcagga aaacgccggt gaaatggagc tggctgacct tgaaggtttc taccgcgatg
     8941 cgaaaaagca ttacgacgaa gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa
     9001 aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc gacatcacca
     9061 tgacgcagaa ccagatcact tacgatcgtc tcaacgtgac gctgacccgt gatgacgtga
     9121 tgggcgaaag cctctacaac ccaatgctgc caggaattgt ggcggatctc aaagccaaag
     9181 gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt aaaaacaagg
     9241 aaggcgaacc gatgggcgtg atcattcaga agaaagatgg cggctatctc tacaccacca
     9301 ctgatatcgc ctgtgcgaaa tatcgttatg aaacactgca tgccgatcgc gtgctgtatt
     9361 acatcgactc ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc cgtaaagcag
     9421 gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg ctgggtaaag
     9481 acggcaaacc gttcaaaacc cgcgcgggtg gtaccgtgaa actggcagac ctgctggatg
     9541 aagccctgga gcgtgcacgt cgtctggtgg cagaaaagaa cccggatatg ccagctgacg
     9601 agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg gatctctcca
     9661 aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt gagggtaata
     9721 ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt aaagcagaaa
     9781 ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt gaagcgcaac
     9841 tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt gaaggcacgc
     9901 cgcatgtaat gtgtgcttac ctgtacgatc tggccggtct gttctctggc ttctacgagc
     9961 actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac
    10021 aactgacagc gaagacgctg aagctgggtc tggatacgct gggtattgag actgtagagc
    10081 gtatgtaatc gatttttcgt gagagtgaag cctgatcagg gttagccgat caggcttttt
    10141 tattgccatc taaatgtatt ctgaaaacgg acatgccatt gttttctcac tgttgagtaa
    10201 gaggccagaa gcgtaatatc caggcccagg aaaacgataa cggttcaatt taaggaatac
    10261 cgaagtgttt aaatttcttg tattaacatt aggcattatc tcttgccagg cttacgcaga
    10321 agatacggtt atagtaaacg accatgacac ttcagccatc aaagattgtt ggcaaaaaaa
    10381 ttcagatgat gatactgaca ttaacgtgat caaatcatgc ctgcgacaag aatacaatct
    10441 cgtcgatgcg caattaaata aagcctatgg tgaagcttat cgttatatag aacaagtgcc
    10501 acgcacaggt gcaaaaaaac ttgatactga acaacttaac ttgcttaaaa aatcacagcg
    10561 agcctggctg gattttaggg acaaagaatg tgaattaatc ctttcaaatg aggatgttca
    10621 ggatttaagt gacccctatt ctgaatcaga atggctatca tgtatgatca tacagaccaa
    10681 tacgcgaact cgccagttgc agctataccg taactctgaa gatttttatc caagcccttt
    10741 gacaagagga taattcacat ctttttggca tgttttgttg caagctattc ctgataaatg
    10801 attgcaacaa aacatccagc cttttgactg agttattaaa catactcgcg agcgcgtaat
    10861 tttttgtctt tcagcgataa ttcacaatca cttcattccg ttgtaccttt agcggtggaa
    10921 ttaaccgccc tgaaccgccc cgggtttcct ggagagtgtt ttatctgtga actcaggctg
    10981 ccagatcatc gtttccgatg gaagcataat aagc
//
LOCUS       DAECNO010000117        10987 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.117, whole genome shotgun sequence.
ACCESSION   DAECNO010000117 DAECNO010000000
VERSION     DAECNO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.117"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..177
                     /gene="relB"
                     /locus_tag="KKO08_003395"
     CDS             <1..177
                     /gene="relB"
                     /locus_tag="KKO08_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBD6319518.1"
                     /translation="KMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKER
                     LRKPKPVRVTLDEL"
     gene            177..338
                     /gene="relE"
                     /locus_tag="KKO08_003396"
                     /pseudo
     CDS             177..338
                     /gene="relE"
                     /locus_tag="KKO08_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
     gene            428..1018
                     /locus_tag="KKO08_003397"
     CDS             428..1018
                     /locus_tag="KKO08_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HBD6319519.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
     gene            1155..1727
                     /locus_tag="KKO08_003398"
     CDS             1155..1727
                     /locus_tag="KKO08_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD6319520.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            1764..3155
                     /locus_tag="KKO08_003399"
     CDS             1764..3155
                     /locus_tag="KKO08_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HBD6319521.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            3200..3616
                     /locus_tag="KKO08_003400"
     CDS             3200..3616
                     /locus_tag="KKO08_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD6319522.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(3935..4591)
                     /locus_tag="KKO08_003401"
     CDS             complement(3935..4591)
                     /locus_tag="KKO08_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HBD6319523.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            4986..6001
                     /locus_tag="KKO08_003402"
                     /pseudo
     CDS             4986..6001
                     /locus_tag="KKO08_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6044..9049)
                     /locus_tag="KKO08_003403"
     CDS             complement(6044..9049)
                     /locus_tag="KKO08_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HBD6319524.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            9213..9770
                     /locus_tag="KKO08_003404"
     CDS             9213..9770
                     /locus_tag="KKO08_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD6319525.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            9953..10813
                     /locus_tag="KKO08_003405"
     CDS             9953..10813
                     /locus_tag="KKO08_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBD6319526.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         2820 a         2650 c         2829 g         2688 t
ORIGIN      
        1 aaaatgggcg taactccttc tgaagcgctt cgtctcatgc tcgagtatat cgctgacaat
       61 gaacgcttgc cgttcaaaca gacactcctg agtgatgaag atgctgaact tgtggagata
      121 gtgaaagaac ggcttcgtaa gcctaagcca gtacgtgtga cgctggatga actctgatgg
      181 cgtattttct ggattttgac gagcgggcac taaaggaatg gcgaaagctg ggctcgacgg
      241 tacgtgaaca gttgaaaaag aagctggttg aagtacttga gtcaccccgg attgaagcaa
      301 acaagctccg tggtatgcct gattgttaca agatggagag catgaaaaac ctcggggcag
      361 ccatcgaatg atgccatcta caggggttaa aataaccacc atcatttcat aacaatacct
      421 gctgactatg aagatctacg tcatcaaaat agcagttcat ggagtcagtc cgatggtctg
      481 gcgtcggctg agaattgctg ccgacacgtc actggccgcg cttcacttca ttttccagat
      541 agtgcagggc tggggggacg accatctcca tcaatttcac atttatggca aagattacgg
      601 tatctcctac gaaggaggca tcggttttgt cgataatccg ttccgggtcg tgattgatga
      661 ttttgctttt gatgctggcg atcgctttac ctatgaatac aatttctttg agcactggct
      721 tcatgacatt cgtgttgagg ccatttatga aaactctacg ctgaaagcgc cgttttgtat
      781 aagcggccat ggtatgcccg gagccacagc tgcggatgag ttcgataaaa ccctggcgtt
      841 tcttgaagct atcgttaatg cggatgatga aacaacggtc ggcgagatcc gcccttttgc
      901 tgacgacctg gatgctgtca ggttcaaccg ccacaaaatc aaccggcaac tgagcaggct
      961 cgaccttgca tctccggtac tggagcctga agttatctgg ctgggccgtc gtcgctgact
     1021 gatgtctata aaggtatacg ttttcagcat gcctctcaga tccccgaaaa aatgtctata
     1081 aacctcccta aatcagtgtt tatggattat tctttaaatc tctatgatgt ttaaagacct
     1141 tccggaggtt ttgcgtgcaa tatgcctaca tcagggtaag ctcggccgat cagaatacgg
     1201 cccgtcagga agaggcattg tcaaaggcgg gttttcagcc tgataaaatt tgtgttgagc
     1261 atgccagcgc gaaggacaca aaccgcccgg ggttacagga actcctgggg caattacgcc
     1321 ctggtgatac gcttctggtt cattccatcg atcgcctttg ccggaatatg tcggatatgt
     1381 gcgctgtcac cacccggctt cgtgaccagg gcgttaccct tatttttctg aaagagcagc
     1441 tgacgttcag cgccggcaca aacaatccga tgcaggaact gcagctgcac atgatgtcgg
     1501 cattcagcca gtttgaacga gccttactga aggaaaggca ggccgacggc atcgccgcga
     1561 aaaaagcacg gggtgaaaaa acagggcgcc ccggcgccga tatcaaaaag atccaggaaa
     1621 ttgacgcact cagaagcaga ggggtcaggc tcaggatcgc ctgcgaccat gcgggccttg
     1681 gcgtttccac atattataag cttcgtcacc agatgaacaa ccagtagccg ggagatagtc
     1741 atccaccaca gcaggaggca atgatgcaac tgacgctcca gatagtcatc accgatgaat
     1801 caggctccag tcgaacggag gaactgatga caatccagaa atcaggggag acccggaacg
     1861 acatcggatt atcggtgtca gaatccaagc tgttgctgaa tacggtccag cagtcggtgg
     1921 tccagctgca ggcagacgaa tatactcaac accatatccg gtgccctcac tgccttgctg
     1981 cgcgcagaat caaaggcaaa cagaaaatac ggtaccggac gctgtttggc gttattccgg
     2041 tatccgggct tcgggtgtac cggtgccgtt gtgaagagag tgatacaaag acagtcagtt
     2101 tgctcagcga ctgggccggc gattattctc acccggcact gaaatatatt gaaacccgct
     2161 gggcctcgat gatctcctat gaaatgacga ctcgtctgct gaaagatatt ctgccggtgg
     2221 gccacagcct gaatgcctca acggtgagga atcatttatg ccaggtggcg cagcgtcttg
     2281 acgctgaggc tgaagctcat tcaggttttc tttccggctg cccccgcgac tgggggaacc
     2341 tgcccaggcc aggaaaaccg cttgtggtgg gtatagatgg tggttatgtt cgcgatcggg
     2401 atgacaaaaa gcgtaatttt gaaattattg ccgggaagtc tttttccgtt ggtgcgcctg
     2461 ctgacacccg ccgcttcggt tttgttcaga aggatgactg tcatccggaa cgcaggctca
     2521 tgacccacct ttcagcgcag ggaatgcagg ccaaccagca gatatttttt ctgtccgacg
     2581 gcgcggataa tctcagggac ctccagttcg gtatgtaccc tgagtcaacg catgtgctgg
     2641 actggtttca tatcaccatg aggctgaagg tgctcatgca atatgcccgg ggactgctgg
     2701 tatcagatcc agaggcgggg agtaaagttc tggcactgct ggaaagcatt aaacgatatc
     2761 tctggcatgg caatgttgtc gctgcgctgg aacatattga taattgcgtt atgtattgcg
     2821 atgaccctga actcagctat cctagcctga aatccctgca aaaacatctg gatgaaatgt
     2881 atacctacat ccggaataat aagatgatga tcccaaacta cggcgaaatg cgtcggtacg
     2941 gagagccggt atcaaccgcg tttgtggaat ccacgatcaa tgaagtgatc gccagacgaa
     3001 tggccaaaaa gcagcagatg cagtggagca gaaaaggggc tcattatttg ttacagaccc
     3061 ggaccgccgt tttgaataac gaactgcagg ataaatttgt ctgctggtat ccgggttttc
     3121 aaagtgacgg gaagggacca gcgatggcag catagctcac gctgccccaa tattttttat
     3181 gctctcggtc gtatggaagc tggaccgcct cggtcgtgat cttcgtcatc tcattaatac
     3241 agtgcacgac ctgaccgcga gggggaccgg tctgaaagtg ctgaccggtc acggggcgac
     3301 tatcgacaca acaacggccg ctggcaagct tgtcttcggt atctttgccg cactggcgga
     3361 gtttgagcgc gaactgattg ctgaaagaac gactgcaggt ctggcatcag caagagcccg
     3421 tgggcggaac ggcggccgac cttataaaat gacgccagtc aaactgcgac tggcaatggc
     3481 atcaatggga caatcagaaa caaaggtcag tacgctatgc caggaacttg ggataacccg
     3541 gcaaactttg tacaggcata tttcccctgt tggtcaactg cgggcggacg ggatcaagct
     3601 gctcaaccgt ggataatttg aacggacgga aaaatctggg gttccggcgt agtaccccta
     3661 tcacctccac atttttgcaa cagtgcctta taaatggcag ccttatgttt acttgccaag
     3721 atgggctacc atatgatgtt ttatactgag caatattaat attatcttta atggaaaaat
     3781 taagcccact aagtgaccca ctgctatatg gctctattgt gaagacgcct gagggtaaaa
     3841 atttatacat ggttgtccct atgtctatta ttgcaaggtt gacaatatta ttcgttttat
     3901 aaatttgagc gtttaaaatc acacatcaaa gcaattagtc aggataaaca acaataccca
     3961 gtgcttcgag aatcagttct tgctgccagg ctgcaatttt gatacctgat gtatcgactt
     4021 tgcggggatc taaaccgtcg agttcggcgt ggcataaatt ggcaccctgt aggctaaatt
     4081 gcccccagac atcttcggaa aaaacacctc gacttaagtc tgactctttc agtgatgcac
     4141 ccgctaggtt cgttcctatc cagcgatttt caaacaactc acatttttct aaacaaaccc
     4201 tctccatatt ggcataggaa agattacatc cagaaataaa tgctgagcaa aagtacatac
     4261 gattactcac ttgatgggca aagtttgttc gggaaaagtt ggcacctttt aaatcacacg
     4321 cacggaactc tataccgtag caattggcat tactgaagtt tgccatcgca agttggcatt
     4381 gttggaaact tgcatcacga agatctgcga catcaaagtg gcagccttcg atatcaccct
     4441 gttcaatgaa cttgcagttg acgaatgtcg tatcacgcaa gttagcacgt cgaaagtcgc
     4501 tgcgaatgaa tgtgcaagcg gtgaaggtga gatcacttaa gtctttatgt gaaaagttgt
     4561 ggtgccgata tgtatgattg taggtttcca tgagtactcc ttgttgaaag gagggctaga
     4621 ctaccattat acatatgcca agacattaaa ataaaagcct gttacatcaa cacattacaa
     4681 cccctcaatt tacctgtttt aagtggtcaa attggcgcgt agagcgccta tcgctcttgc
     4741 attggcacgc ggccctgttg aaaaaagtta ttcgctttag atagggttag gagagccgtg
     4801 cttcgccggt aaaaaatcgg actatccgac gcagggcgga ctcatcggcg gagaagcaaa
     4861 ctttgactat atctccctac acttggaaga gggctagact ggccccatag atcaccagac
     4921 acttcgactc tcttaaaata agaggtagtc ttggaactat gtttaatacc agtcagaacg
     4981 ataagatggt tgaagtgtta tcaggacctg agcggcgtcg gcgtcgtaca ccgcaggaaa
     5041 aaattgccat tattcagcag actatggagc ccggtatgac cgtgtctcat gtcgcgcgcc
     5101 tgcatggcat caatgcaaac cagatattca aatggcgcaa gcaatattaa gacggttcac
     5161 tgacggctgt tgcatcgggc gaggaagtgg tgccggcctc cgaactcgct gccgccaata
     5221 agcaaattcg cgaactccag cgccttctgg gcaaaaagac gatggaagcc gagatactca
     5281 aagaggccgt ggagttcggc cgggcaaaaa aatggattgc gcatgcgccc tcgttgcctg
     5341 ggaacgacga ataagcgacg tttgcagaag tcttggcgtg tcgcgtgcgc agttgagcat
     5401 tcgggttcac cggccatctg actgacagga tcgcagacgg cagccccgtt ctgacgatac
     5461 caccgtattg tcccggataa acatggcggt ggctgacctg ccgacgtatg gttatcgccg
     5521 tgtgtgggcg ctgctgcgac gggagtcgga gcgggacggc ctgcctgtgg ttaatgcaaa
     5581 gcgggtgtat cgcattatga gaacgcataa tctgcttctt gaacgtaaac ccgctgatcc
     5641 ttgccgtaag cgggcccata aggggcgcgt agcagtagcg gaaagtaacc ggcgctggtg
     5701 ctcagatggc tttgagttcc gctgtgataa tggcgaaaag ctgcgggtga ccttcgcgca
     5761 ggactgctgc gacagggaga tcattgactg ggcagcgagc accggaggct atgacaaaga
     5821 aacggtgcag gatgtcatgc tgggtgcggt ggaaaaacgc ttcggaaggc agttgccaac
     5881 agtgccggcg ttcgcccggt tgctgggact ggagccttgt acgacagcag tgcgaagccc
     5941 ggaaagcaat ggcatagcag aaagcttcgt gatgacgata aaacgggact acatcagcat
     6001 caggatttta ggggtctgac gctcagtgga acgaaaactc acgttaagca acgttttctg
     6061 cctctgacgc ctcttttaat ggtctcagat gtcctttggt caccagttct gccagcgtga
     6121 aggaataatg gccgagcata ttgatatgtc cgtggcaaag cggggagagg cgtgcgatat
     6181 cttcatcatt cagtgtttca ccctgcgccc ggagatgatc cagggctgcc tgcatataaa
     6241 tagtgttcca taacacgacg gcgttagtga ccagccccag tgtgcccagt tgatcttcct
     6301 gaccgtcggt atatcgtttt cttatctcac ctttttgacc gtgacagatg gctctggcaa
     6361 cggcatggcg actttctccc cgattaagct gggtcagaat gcgccggcgg taatcttcat
     6421 catcaatata attaagcaga tacagcgttt tgttgatgcg ccccacttca atgattgcct
     6481 gagtcagtcc ggaaggacgt tcacttttca gcaatgaacg gaccagcact gaaacctgta
     6541 ctttgcccag cttcagggag ccagcggtcc ggatcatttc gtcccactga aggactattt
     6601 ttcggggatc tgattgccct ctggcaatat cattcagcac gccatagtcg gcatcatggt
     6661 ccattcgcca gaaaaccgaa gcaccggcat cagccaggcg tggagaaaac tggtatccca
     6721 gcagccagaa aaggccaaag acaagttcgc tggcacctgc tgtatcggtc ataatttcgg
     6781 ttggattcag cccggtctcc tgttccagaa gaccttccag cacaaagata gagtccctca
     6841 gcgtccccgg tataacgatg ccatgaaagc cggaatactg atcggacaca aagttgtacc
     6901 aggtgatccc tctgttatta ccaaagtatt tgcggttcgg tccggcattg attgttctga
     6961 ctggcgtaac aaagcgcatt ccatctgcag atgccacttc tcctccaccc catatctgtg
     7021 ccagtggcag cgttgcctga aaatcaacca gtctggcatt agcgctggtg atagtttcag
     7081 cccgcagata gttcgctttt gtccagttca gccggtgtcg ggtcagtgca ggaacatttg
     7141 atctgatcag tggttccaga ccgatattgc aggcttcagc catcagcacg gcgctgatgc
     7201 tgacgggcag atcatcaact ctggcactgg cttcactagc atggaaaaac tcatcagcaa
     7261 atccggtatg ggcgttaatt tcgagcagca actccgttaa atccaccgga gggagtagat
     7321 cactgatcat tttgctcagt cgtttcagac tgtccggctc atcaagactg gcgaggggag
     7381 aaattgtcaa ccggggcttc gggccagaaa catcgagttc gacagcctca ttttcgcaaa
     7441 gacgtgcagc aacctgtctg taacgactat caagctgatg acccagagat tttattgctt
     7501 cctgcgggtc tgtcgggtgc cccaaagaac gataaacctt aatccggttt gcctgccagt
     7561 cagcaccctg tagtaatctt gcacgaggat ctccccaccg gttactgccg gtaacgtaga
     7621 catccctccg cctcagacta tcctgcagtt tactgagaaa gcagagcgtg tatcccctgc
     7681 gggtgatatg tttttccttg ttaatcacca gccgtttcca tgaccgactg ataatttccg
     7741 ttggtgcgtc gtcaaaaaac tgccgccgtg agctgaactc ccggctgagg tagtcacagg
     7801 cattcagagt ggtaaccccg gcaggtgcgg atgaaaattt aacggtattc agcagatggg
     7861 gcaggaaacg acgaacgcgc ccgtactgct ccaccatttc ttcatgaaaa ttatcgtctg
     7921 agggccgggc aatttcacgg acaagcgtga tgatttcagc cagcttttgc cttgggatgt
     7981 agctgaacac ctcagcacga atcgattcgt ccggtgtttc ttctttcagc aggtacgaac
     8041 atgcgctggc gagcgccaat gcagatttat ccagatcctt cagcgagcgg agccgttttt
     8101 tctgcccaat ctttctggcg tcacggatga taacggccag catggcgtcc agaacgtcca
     8161 atgcatcatc cagcgccagc gtttcccatg caaggacaaa ggcaaccaga accgccatcc
     8221 ttttctgcgg tgacatcctg gcaatattga acaccgaagt cataccagca taacgtgcga
     8281 gatttttcag gcgcacagcc gggagtgtac tcaggttttc agcatgcagg ccaaaatcgt
     8341 tcagagtttt ccagcgttca attgcttcat taaacgccgg accactgatg gtcacagggc
     8401 cctttttcag tgattccagt aaagacaggc ggctgcaatc agttggcccc agcagcatct
     8461 ccagctgtga acgctgttcg gctgacggta tcagtgccag tttgttccac aggcgcaacg
     8521 tcgccttttc ccttacctct gaaatcaacc gggtcagcgt agtggctccg gggagaataa
     8581 tacgatgttg cataagccac cctgtcgcca gatcgaaaag caggccagga cgttcgttgc
     8641 ttatccagct ccgggtatat aaaagacggg taaggcgaaa tgtccagggc caggcaaatt
     8701 cacgatactg atagtgctga cgtatcagcg ctgcatgctc acggcgggta ttttccctct
     8761 gaccgtattc tgcaagaacg gtgatatcac gaatcccgag ctgtctggcg gtaaaatgcc
     8821 ggacgccgga aggaatatga ttcatatcgg tgaggaaggt gcccagaaaa cggacacatc
     8881 caatttgcag ggcaatgccc agacggttgt gatcacctct gctttttccg ataaattcct
     8941 tgtctgcttc atcaaggtga aaatatcgtg ccagctgaag ctcatccggt tcaccggtga
     9001 atctgccata gctttcagtc tgctcagtgg tcagaaagtc aacgggcata tcggcctccc
     9061 tgcctgacgg gcatttagta acatttttcc aaccgtacga aatgttataa attatcggac
     9121 atcgtaaaac tgttacatta atatgtctat taaatcgtaa atttgtaata atagacatga
     9181 gttgtccgat attcgattta aggtacattt ttatgcgact ttttggttac gctcgggtct
     9241 caaccagtca gcagtctctt gatcttcagg tcagagcact caaagacgca ggtgtgaaag
     9301 caaaccgtat atttaccgat aaggcatccg gcagttcaac agaccgggaa gggctggatt
     9361 tgctgaggat gaaggtggag gaaggtgatg tcattctggt taagaagctc gaccgtcttg
     9421 gccgcgacac tgccgatatg atccaactga taaaggaatt tgacgctcag ggcgtggcag
     9481 tccggttcat tgatgacggg atcagtaccg acggtgatat ggggcaaatg gtggtcacca
     9541 tcctgtcggc tgtggcacag gctgaacgcc ggaggatcct agaacgcacg aatgagggcc
     9601 gacaggaagc aaagctgaaa ggaatcaaat ttggccgcag gcgtaccgtg gacaggaacg
     9661 tcgtgctgac gcttcatcag aagggcactg gtgcaacgga aattgctcat cagctcagta
     9721 ttgcccgctc cacggtttat aaaattcttg aagacgaaag ggcctcgtga tacgcctatt
     9781 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     9841 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9901 catgagacaa taaccctggt aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9961 tcaacatttt cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    10021 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    10081 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10141 ttttccaatg atgagcactt ttaaagttct gctatgtggt gcggtattat cccgtgttga
    10201 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10261 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10321 tgccataacc atgagtgata acactgctgc caacttactt ctgacaacga tcggaggacc
    10381 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10441 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
    10501 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10561 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10621 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10681 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10741 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10801 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10861 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10921 cccttaacgt gagttttcgt tccactgagc gtcagacccc taaaagaact ttccgctaag
    10981 cgataga
//
LOCUS       DAECNO010000118        10956 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.118, whole genome shotgun sequence.
ACCESSION   DAECNO010000118 DAECNO010000000
VERSION     DAECNO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.118"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            241..2493
                     /locus_tag="KKO08_003406"
     CDS             241..2493
                     /locus_tag="KKO08_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319527.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            2829..3806
                     /gene="csiD"
                     /locus_tag="KKO08_003407"
     CDS             2829..3806
                     /gene="csiD"
                     /locus_tag="KKO08_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBD6319528.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3826..5094
                     /gene="lhgO"
                     /locus_tag="KKO08_003408"
     CDS             3826..5094
                     /gene="lhgO"
                     /locus_tag="KKO08_003408"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBD6319529.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5117..6565
                     /gene="gabD"
                     /locus_tag="KKO08_003409"
                     /pseudo
     CDS             5117..6565
                     /gene="gabD"
                     /locus_tag="KKO08_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6573..7859
                     /gene="gabT"
                     /locus_tag="KKO08_003410"
     CDS             6573..7859
                     /gene="gabT"
                     /locus_tag="KKO08_003410"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBD6319530.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8097..9497
                     /gene="gabP"
                     /locus_tag="KKO08_003411"
     CDS             8097..9497
                     /gene="gabP"
                     /locus_tag="KKO08_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBD6319531.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9518..10180
                     /gene="csiR"
                     /locus_tag="KKO08_003412"
     CDS             9518..10180
                     /gene="csiR"
                     /locus_tag="KKO08_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBD6319532.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10181..10630)
                     /gene="kbp"
                     /locus_tag="KKO08_003413"
     CDS             complement(10181..10630)
                     /gene="kbp"
                     /locus_tag="KKO08_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBD6319533.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10714..10872)
                     /locus_tag="KKO08_003414"
     CDS             complement(10714..10872)
                     /locus_tag="KKO08_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBD6319534.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2789 a         2698 c         2784 g         2685 t
ORIGIN      
        1 catatagcaa tatattcttt catagatctt attctgctaa tcattagttt cgtatgcgcg
       61 atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt taaacattat
      121 ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac tttcacaaca
      181 attaaacatt tttatgtttt cgtatacatc atattactct accattagag gaacgttatt
      241 atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac attgttatca
      301 tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata tctaaactgt
      361 ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga cagagaaatt
      421 atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga ttataaaatt
      481 tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag tagcattaca
      541 gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac aatggttgaa
      601 agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac tcacattata
      661 tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat tcctgactat
      721 gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca gcaagaaaca
      781 tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa atctcttact
      841 gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga agcgattctt
      901 catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt tgcacgttcc
      961 cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg accagaaact
     1021 cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct ttgtaccatc
     1081 cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc taaaaaattt
     1141 ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg gagtggtaaa
     1201 aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga tatagaaagt
     1261 ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga tccagtaaca
     1321 aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga cttcgaagca
     1381 agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa agaaaagagt
     1441 ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt gcaaaatcaa
     1501 tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc agcaaaacac
     1561 tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta taatgagcga
     1621 ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat gaattacatg
     1681 gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct ttatgaaaga
     1741 gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc cttttcattc
     1801 gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag catcccatca
     1861 gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa tcttttcaat
     1921 gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat ggccgctctc
     1981 ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac gactgaaatt
     2041 aaaaattatg atgggtccac gcgtgtcggt ggtgaggcgt ggcttaataa aggttgctct
     2101 acctatcagc agctctacaa tgaattccac gcattatata tagataaagc aggaatatgg
     2161 agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg tggggattct
     2221 gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg aaacgaagaa
     2281 gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat ggggagtaat
     2341 agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa agtatttacg
     2401 cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct tttagggaaa
     2461 acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc cccgataaaa
     2521 cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc atttactgtt
     2581 ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt tgctttagtt
     2641 attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta atttgttgct
     2701 tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga aatgtagata
     2761 tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc ataagaggat
     2821 cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca ggccaggact
     2881 atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca
     2941 ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg caggcgctgg
     3001 agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg tgtgcgaatc
     3061 agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg ctgttgatca
     3121 atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg gcaacggcgg
     3181 tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat tacgcgcgtt
     3241 tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac cgcgtgatgg
     3301 aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg atgatgaaaa
     3361 tcgatgagca aaacatgcag ggtggaaatt cgttgctgct gcatctcgat gactgggaac
     3421 atctggacca ctatttccgc catccactgg cgcgtcgccc gatgcgcttt gccgcgccgc
     3481 cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat caacagggtc
     3541 gcccggtgat gcgctatatc gaccagttcg tccagccaaa agattttgaa gaaggcgtgt
     3601 ggttgagcga gctttccgat gccattgaaa ccagcaaagg cattctttct gtacccgttc
     3661 ccgttggcaa attcctgttg ataaacaacc tgttctggct gcacggtcgc gaccgcttta
     3721 ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg tggctatttc gcttacgcca
     3781 ctcaccacta ccagacgcat cagtaagcgc aaaggaattg agcggatgta tgattttgtg
     3841 attattggcg gcggcatcat cggcatgtcg accgccatgc aactgattga tgtttatccg
     3901 gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg cctgtcacca gacgggccac
     3961 aacagcggcg tgatccatgc cggggtctat tacacgcccg gcagcctgaa ggcgcagttt
     4021 tgcctggcgg ggaaccgtgc caccaaagcc ttttgcgatc aaaacggcat tcgctatgac
     4081 aactgcggca agatgctggt cgccacgtct cagctcgaaa tggaacggat gcgcgcctta
     4141 tgggaacgca cagcggcgaa cggtatcgag cgcgagtgga taaacgccgt ggaactgcgc
     4201 gagcgcgaac cgaatatcac cgggcttggt ggcatttttg tgccgtccag cggcattgtc
     4261 agctaccgcg aagtgacggc ggcaatggca aaaatcttcc aggccagagg cggcgagatt
     4321 atttataacg ccgaagtcag cgccctcagt gagcataaaa acggcgtggt gatacgcacc
     4381 cgtcagggcg gtgaatatga agcatcgacg ctgattagct gttccgggct gatggctgac
     4441 cggctggtga aaatgctcgg cctcgaaccg ggctttatta tctgcccgtt ccgtggcgag
     4501 tatttccgcc ttgcgccgga gcataaccag attgttaacc acctgattta ccccattccc
     4561 gaccccgcga tgccattttt gggcgtgcat ctcacccgca tgatcgacgg cagcgtgacc
     4621 gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg cgacttctca
     4681 tttagtgaca cgctggagat tttgagctcg tcggggattc gccgggtgct gcaaaaccat
     4741 ctacgctcag gactgggcga gatgaaaaac tcgctgtgca aaagcggcta tctgcggctg
     4801 gtgcaaaagt attgtccccg gctttcgtta agcgatctcc agccctggcc cgccggtgtg
     4861 cgggcgcagg cagtatcgcc ggacggcaag ctgattgacg attttctgtt tgtcaccacc
     4921 ccacgcacga tccacacctg caatgcgccc tccccggcag cgacatcagc aattcctatt
     4981 ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc
     5041 acgctgcgag cggcacgtag tgtggatgcc ttacacgccg catttaatca ataacctttg
     5101 aaaacaggac gtaacaatga aacttaacga cagtaactta ttccgccagc aggcgttgat
     5161 taacggggaa tggctggacg ccaacaatgg tgaagtcatc gacgtcacca atccggcgaa
     5221 cggcgacaag ctgggtagcg tgccgaaaat gggcgcagat gaaacccgcg ccgctatcga
     5281 cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc gccaaagaac gcgccaccat
     5341 tctgcgcaac tggttcaatt tgatgatgca gcatcaggac gatttagcgc gcctgatgac
     5401 cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa atcagctacg ccgcctcctt
     5461 cattgagtgg tttgctgaag aaggcaaacg tatttatggc tacaccattc ctggtcatca
     5521 ggccgataag cgcctgattg ttatcaagca gccgattggc gtcaccgccg ccatcacgcc
     5581 gtggaacttt ccggcggcga tgattacccg taaagccggt ccggcgctgg cggcaggctg
     5641 caccatggtg ctgaagcccg ccagtcagac gccgttctct gcgctggcgc tggcggaact
     5701 ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt cggcaggcgc
     5761 ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa ctgtcgttta ccggttagac
     5821 cgagattggc cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga
     5881 gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata aagccgtgga
     5941 aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt
     6001 atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa ttacagcagg cggtgagcaa
     6061 actgcacatc ggcgacgggc tggagaaagg cgtcaccatc gggccgctga tcgatgaaaa
     6121 agcggtagca aaagtggaag agcatattgc cgatgcgctg gagaaaggtg cgcgtgtggt
     6181 ttgcggcggt aaagcgcacg aacgcggcgg caacttcttc cagccaacca ttctggtgga
     6241 cgttccggcc aacgccaaag tgtcgaaaga agagacgttc ggccccctcg ccccactgtt
     6301 ccgttttaaa gatgaagccg atgtgattgc acaagccaat gacaccgagt ttggtcttgc
     6361 cgcctatttc tacgcccgtg atttaagccg cgtcttccgc gtcggcgagg cgctggagta
     6421 cggcatcgtc ggcatcaata ccggcattat ttccaatgaa gtggccccat tcggcggcat
     6481 caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt acttagaaat
     6541 caaatatatg tgcatcggtc tttaactgga gaatgcgaag gaacagcaat aaagagttaa
     6601 tgcagcgccg cagtcaggcg attccccgtg gcgttgggca aattcacccg attttcgctg
     6661 accgcgcgga aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt gatttcgcgg
     6721 gcgggattgc ggtgctcaat accgggcacc tgcatccgaa ggtggtggcc gcggtggaag
     6781 cgcagttgaa aaaactgtcg cacacctgct tccaggtgct ggcttacgag ccgtatctgg
     6841 agctgtgcga gattatgaat cagaaggtgc cgggcgattt cgccaagaaa acgctgctgg
     6901 ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat cgcccgcgcc gccaccaaac
     6961 gtagcggcac catcgctttt agcggcgagt atcacgggcg cacgcattac accctggcgc
     7021 tgaccggcaa ggtgaatccg tactctgcgg gcatggggct gatgccgggt catgtttatc
     7081 gcgcgcttta tccttgcccg ctgcacggca taagcgagga tgacgctatc gccagcatcc
     7141 accggatctt caaaaatgat gccgcgccgg aagatatcgc cgccatcatg attgagccgg
     7201 ttcagggcga aggcggtttc tacgccgcgt cgccagcctt tatgcagcgt ttacgcgctc
     7261 tgtgtgacga gcacgggatc atgctgattg ccgatgaagt gcagagcggc gcggggcgta
     7321 ccggcacgct gtttgcgatg gagcagatgg gcgtggcgcc ggatcttacc acctttgcga
     7381 aatcgatcgc aggcggtttc ccgctggcgg gcgtcaccgg gcgcgcggaa gtgatggacg
     7441 ctgttgcacc gggcggtctg ggcggcacct atgccggtaa cccgattgcc tgcgtggctg
     7501 cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct gcaaaaagcc aacgatctgg
     7561 ggcagaagct aaaagacgga ttgttggcga tagccgaaaa acacccggag atcggcgacg
     7621 tacgcgggct gggatcgatg attgccatcg agctgtttga agatggcgat cacagcaaac
     7681 cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg cgataagggc ctgattcttc
     7741 tctcctgcgg cccgtattac aacgtgctgc gcatccttgt accactcacc attgaagacg
     7801 ctcagatccg tcagggtctg gagattatcg gtcagtgttt tgatgaggcg aagcagtagc
     7861 accgccccca tgcaggaggc gacgctgcgc gtcttgtccg gcctacgaga atccaagtcg
     7921 gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa caggaataaa tctatgtcgg
     7981 ccggataagg cggagccgct ctccggcaaa aagaatcaat aacaattaaa cgcgtgaccc
     8041 gacgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     8101 ggcaatcatc gcaaccacac gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     8161 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     8221 aagcggaccc ggcggtatta ctggcctatc tgttcgccgg attactggtg gttatgatta
     8281 tgcggatgct ggcggaaatg gcagttgcta cgcccgatac cggttcgttt tccacctatg
     8341 ccgataaagc cattggccgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     8401 gggtactggt tatcccactg gaagccaaca tcgccgccat gatcctgcac tcgtgggttc
     8461 caggcattcc catctggtta ttttccctcg tcattaccct cgccttaact ggcagtaatt
     8521 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     8581 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     8641 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8701 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8761 ttgccgccgc ggaatccgac atgccggaaa aacatattgt ccgcgccacc aactcggtta
     8821 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8881 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8941 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     9001 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     9061 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     9121 ccggcgcagc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     9181 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     9241 agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga aattcgcttg cgaatgtggc
     9301 ttgatccctg gctcacctgg ctggtcatca gctttattac ctttgtgttg gtagtgatgc
     9361 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     9421 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     9481 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     9541 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     9601 gatgagttta ctgacatcgc gttatgcact tggcgttggg ccgttgcagg aagcactttc
     9661 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9721 tatgtcagag caggagctgc tcgatatttt cgacgcccgc gccaatatgg aagcgatgct
     9781 ggtcagtctg gcgattgccc gcggtggcga tgaatgggag gcagacgttc tcgcaaaagc
     9841 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9901 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9961 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat cgatttatct ggctgcggcg
    10021 aacggtgctt tcggtggaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
    10081 ggcggtactg ccgcgagata cagcgcgcgc cagtgagtta atgcgccagc atttactgac
    10141 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
    10201 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
    10261 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
    10321 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
    10381 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
    10441 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
    10501 cggcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
    10561 tatcgtgctg acctgtaacc gcatcccaga gtttttctcc ggcatcttta acaaaattga
    10621 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10681 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10741 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10801 accgaaccct ttaccgagca gcacgccgat cggcggcaga atgatggtga tgacgattct
    10861 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10921 ctcttaagcg tagtaatttt agcggagcct gccagt
//
LOCUS       DAECNO010000119        10925 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.119, whole genome shotgun sequence.
ACCESSION   DAECNO010000119 DAECNO010000000
VERSION     DAECNO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.119"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..203
                     /locus_tag="KKO08_003415"
     CDS             <1..203
                     /locus_tag="KKO08_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319535.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="KKO08_003416"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="KKO08_003416"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBD6319536.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="KKO08_003417"
     CDS             2102..2818
                     /locus_tag="KKO08_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBD6319537.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="KKO08_003418"
     tRNA            complement(3274..3349)
                     /locus_tag="KKO08_003418"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="KKO08_003419"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="KKO08_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="KKO08_003420"
     tRNA            5082..5157
                     /locus_tag="KKO08_003420"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="KKO08_003421"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="KKO08_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBD6319538.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="KKO08_003422"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="KKO08_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBD6319539.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="KKO08_003423"
     tRNA            7492..7567
                     /locus_tag="KKO08_003423"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="KKO08_003424"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="KKO08_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBD6319540.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="KKO08_003425"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="KKO08_003425"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBD6319541.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="KKO08_003426"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="KKO08_003426"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBD6319542.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="KKO08_003427"
     CDS             complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="KKO08_003427"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBD6319543.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3035 a         2782 c         2364 g         2744 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcaggc
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaaatt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagataattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgctggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccgggtgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ctcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacagaacct
    10921 gtgaa
//
LOCUS       DAECNO010000120        10904 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.120, whole genome shotgun sequence.
ACCESSION   DAECNO010000120 DAECNO010000000
VERSION     DAECNO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.120"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KKO08_003428"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="KKO08_003428"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBD6319544.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KKO08_003429"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="KKO08_003429"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBD6319545.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KKO08_003430"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="KKO08_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBD6319546.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="KKO08_003431"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="KKO08_003431"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBD6319547.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="KKO08_003432"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="KKO08_003432"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBD6319548.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="KKO08_003433"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="KKO08_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBD6319549.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="KKO08_003434"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="KKO08_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBD6319550.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="KKO08_003435"
     CDS             complement(8064..9206)
                     /locus_tag="KKO08_003435"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBD6319551.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="KKO08_003436"
     CDS             complement(9215..10228)
                     /locus_tag="KKO08_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HBD6319552.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..>10904)
                     /gene="rfaY"
                     /locus_tag="KKO08_003437"
     CDS             complement(10253..>10904)
                     /gene="rfaY"
                     /locus_tag="KKO08_003437"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBD6319553.1"
                     /translation="FYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLKVYA
                     PKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIML
                     IEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLS
                     GKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK"
BASE COUNT         3096 a         2442 c         2264 g         3102 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttgg aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctgcc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgctaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaag
//
LOCUS       DAECNO010000121        10901 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.121, whole genome shotgun sequence.
ACCESSION   DAECNO010000121 DAECNO010000000
VERSION     DAECNO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.121"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="KKO08_003438"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="KKO08_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBD6319554.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KKO08_003439"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="KKO08_003439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBD6319555.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="KKO08_003440"
     CDS             complement(2271..3686)
                     /locus_tag="KKO08_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6319556.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KKO08_003441"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="KKO08_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBD6319557.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KKO08_003442"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="KKO08_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KKO08_003443"
     CDS             complement(9875..>10901)
                     /gene="mdtA"
                     /locus_tag="KKO08_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBD6319558.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREY
                     AKKGARS"
BASE COUNT         2510 a         3070 c         3003 g         2318 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggtgaggta a
//
LOCUS       DAECNO010000122        10748 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.122, whole genome shotgun sequence.
ACCESSION   DAECNO010000122 DAECNO010000000
VERSION     DAECNO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.122"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(133..1482)
                     /gene="lysC"
                     /locus_tag="KKO08_003444"
     CDS             complement(133..1482)
                     /gene="lysC"
                     /locus_tag="KKO08_003444"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBD6319559.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2007..3656
                     /gene="pgi"
                     /locus_tag="KKO08_003445"
     CDS             2007..3656
                     /gene="pgi"
                     /locus_tag="KKO08_003445"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBD6319560.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4118..4360
                     /locus_tag="KKO08_003446"
     CDS             4118..4360
                     /locus_tag="KKO08_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319561.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4474..5112
                     /locus_tag="KKO08_003447"
     CDS             4474..5112
                     /locus_tag="KKO08_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBD6319562.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5109..5846
                     /locus_tag="KKO08_003448"
     CDS             5109..5846
                     /locus_tag="KKO08_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBD6319563.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5846..7942
                     /locus_tag="KKO08_003449"
     CDS             5846..7942
                     /locus_tag="KKO08_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBD6319564.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7989..8267)
                     /locus_tag="KKO08_003450"
     CDS             complement(7989..8267)
                     /locus_tag="KKO08_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319565.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8268..8411
                     /locus_tag="KKO08_003451"
     CDS             8268..8411
                     /locus_tag="KKO08_003451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319566.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8537..8947
                     /gene="psiE"
                     /locus_tag="KKO08_003452"
     CDS             8537..8947
                     /gene="psiE"
                     /locus_tag="KKO08_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBD6319567.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8991..10466)
                     /gene="xylE"
                     /locus_tag="KKO08_003453"
     CDS             complement(8991..10466)
                     /gene="xylE"
                     /locus_tag="KKO08_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBD6319568.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2728 a         2741 c         2778 g         2501 t
ORIGIN      
        1 gtttttctgc tcattattag ctccttctga tgccattcta tttcaggaag gagtgtccgt
       61 taaactcagg ctacctcata gtgacaagaa aatcaatacg gcccgaaata tagcttccag
      121 gccatacagt atttactcaa acaaattact atgcagtttt tgcaccacct gctcggcatc
      181 ttcgccgggc accaggaagc acaggttatg gctggatgcg ccgtaacaaa tcatgcgaat
      241 gttgaacggt tccagtacgc cgaatacctc tttgccaacg ccgcaggctt ttgacaggtc
      301 attgccaatc aacgcgacca gcgccagacc ttcttccacc tccacccggc acagtgcgga
      361 aagctccatc agcagagatt gcgtcagcaa cgtatcgcca gtggaggttg aaccggtggt
      421 atcaagggtt aatgccacgc tcacttctga cgtggtgatt aagtctaccg aaatattatg
      481 ccgcgcgagg atgccgaaaa cttccgcgag gaagccgcga gaatgcagca tattcaggct
      541 gtgcaaagtg agcagagtct gattgcgacg aagcgccagc gcgcggaaca gcggcggatt
      601 ttcagtttta ttgcacacca gcgtaccacc tgcgcgtggg tctttgctgg agccgacaaa
      661 gaccgggata tcgctgcgta ctgcgggtag caacgttgcc ggatgcagta cttttgcacc
      721 aaaagttgcc atctctgccg cttcggcaaa cgcgatttca tcaatgcgtt ttgctgcgga
      781 aactacgcgt ggatcggtgg tgtagatgcc cgggacgtcg gtccagatat caacacgaga
      841 tgcgtgtaaa gcctccgcca gcaaggctgc cgtataatcg ctgcctccac ggccaagcgt
      901 cgttgtacga cctttatttt cgctaccgat aaatccctgg gtgatcacta agccttcatt
      961 gaggcgtggg agcagctgca gcgcggccag ttccgccagc gcggctacat ctggctctgc
     1021 acgaccaaat cggtcgttgg tacgcatcac tttacgtaca tcaaaccact gtgcctgaac
     1081 atcgcgttcg cgcaggattt cgacaaacag cagggtcgac atcagctcgc cgtggctgac
     1141 cagctcatct gtcagcgccg gagacgttgc cagcgccgcc gcttctgcca gaacagtaat
     1201 gttctccagc agacgttcaa tctcttcacg gataacgttc gggtaacgca gacgttccag
     1261 aatggcaaac tggatgttgc gaatagcgtc gagtttttcg aatcgctcgc caagttccag
     1321 cccttccgct aaagcgacca gcagattagt gataccagca gaagccgaga ggactactaa
     1381 acgcacgttg gcatcagaaa gcacaatatc agcgctgcgg ttcatggcgt caaaatcagc
     1441 tacgctggta ccgccaaatt tggagacaac aatttcagac ataactacct cgtgtcaggg
     1501 gatccatttt cagccttggc acaagggaag agcggaagac gggtgggcgc agagcgatac
     1561 ttcgctacta ttttcaccca gaagtgctcc accacttgcg aaacgcccga ctgcgaacgc
     1621 ttctggtgac aacccagggg attcagcccc tgtagccgat gatgaacgtg gccagccgtt
     1681 caatcacctc ggcgatgcac cccctcaggt gttatcacag gactggctcc tccaacaccg
     1741 ttacttgggc aacgcgcctc ttctggcctg cgctagcgca ggtagtacat ttataaataa
     1801 agggtgagcg gggcggttgt caacgacggg gtcatgcgga tttttcatcc actcctggcg
     1861 gtcagtagtt cagctaataa atgcttcact gcgctaaggg tttacactca acattacact
     1921 aacggcacta aaaccatcac atttttctgt gactggcgct acaatcttcc aaagtcacaa
     1981 ttctcaaaat cagaagagta ttgctaatga aaaacatcaa tccaacgcag accgctgcct
     2041 ggcaggcact acagaaacac ttcgatgaaa tgaaagacgt tacgatcgcc gatctttttg
     2101 ctaaagacgg cgatcgtttt tctaagttct ccgcaacctt cgacgatcag atgctggtgg
     2161 attactccaa aaaccgcatc actgaagaga cgctggcgaa attacaggat ctggcgaaag
     2221 agtgcgatct ggcgggcgcg attaagtcga tgttctctgg cgagaagatc aaccgcactg
     2281 aaaaccgcgc cgtgctgcac gtagcgctgc gtaatcgtag caataccccg attctggttg
     2341 atggcaaaga tgttatgcca gaagtcaacg cggtgctgga gaagatgaaa accttctcgg
     2401 aagcgattat ctccggtgag tggaagggtt ataccggcaa agcaatcact gacgtagtga
     2461 acatcgggat cggcggttct gacctcggcc cgtacatggt gaccgaagca ctgcgtccgt
     2521 acaaaaacca cctgaacatg cactttgttt ctaacgtcga tgggactcac atctcggaag
     2581 tgctgaaaaa agtaaacccg gaaaccacgc tgttcctggt agcatctaaa accttcacca
     2641 ctcaggaaac catgaccaac gcccatagcg cgcgtgactg gttcctgaaa gcggcaggtg
     2701 atgaaaaaca cgttgcgaaa cattttgcgg cgctctcaac caatgccaaa gccgttggcg
     2761 agtttggtat tgatactgcc aacatgttcg agttctggga ctgggtcggc ggccgttact
     2821 ctttgtggtc agcgattggc ctgtcgattg ttctctccat cggctttgat aacttcgttg
     2881 aactgctttc tggcgcacac gcaatggaca agcatttctc caccacgcct gccgagaaaa
     2941 acctgcctgt cctgctggcg ctgattggca tctggtacaa caatttcttt ggtgcggaaa
     3001 ctgaagcgat tctgccgtat gaccagtata tgcaccgttt cgcggcgtac ttccagcagg
     3061 gcaatatgga gtccaacggt aagtatgttg accgtaacgg taacgttgtg gattaccaga
     3121 ctggcccgat tatctggggt gaaccaggca ctaacggtca gcacgcgttc taccagctga
     3181 tccaccaggg aaccaaaatg gtaccgtgcg atttcatcgc cccggctatc acccataacc
     3241 cgctctctga tcaccaccag aaactgctgt ctaacttctt cgcccagacc gaagcgctgg
     3301 cgtttggtaa atcccgcgaa gtggttgagc aggaatatcg cgatcagggt aaagatccgg
     3361 caacgcttga ctacgtggtg ccgttcaaag tgttcgaagg taaccgcccg accaactcca
     3421 tcctgctgcg cgaaatcact ccgttcagcc tgggtgcgtt gattgcgctg tatgagcaca
     3481 aaatctttac tcagggcgtg atcctgaaca tcttcacctt cgaccagtgg ggcgtggagc
     3541 tgggtaaaca actggcaaac cgtattctgc cagagctgaa agatgataaa gaaatcagca
     3601 gccacgatag ctcgaccaat ggtctgatta accgctataa agcgtggcgc ggttaatcat
     3661 catgcgtgcc tgatgccgat atgtaggccg gataaggcgt ttacgccgca tccggcaacc
     3721 ggagcctgat gccaatatcg acattaatgc atagtatgag gagttaaata accttaaaaa
     3781 caatgccgac aataagtcgg cattgtttta tcagataaat ccccttgtct gtgatttaac
     3841 ggaaatcata ccgtgaggtt aatcctaaaa tagattttta atcgttgttt atttcggaga
     3901 atacgcagat taattacttt tgtttttatt ttaagcttat gatttttatt gttatttaaa
     3961 tataagttga aacttatatt tgatattcat tccaattatc ctaaaactcc atcgctaatt
     4021 ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg aaatgttttt
     4081 gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc tgtatggcat
     4141 ttttgccata tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac aggtgggcga
     4201 agcggcagag tcggcagcaa cgtcggtttc ggcggggagt tcctccgcga ccagcgtcag
     4261 caccgtaagc tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg atggttctaa
     4321 taccgggacc atcacaacca cgaccaccag tacccagtaa taaagtatgt atccccaaaa
     4381 taatttgagt cattgcatct gtggcttgaa gtatgaaggg atttaccata accacactcc
     4441 ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gacctgcact cattcttatc
     4501 tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc actgtgggac
     4561 agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa tatgccctac
     4621 gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct ggccttcgct
     4681 gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac acaacatggt
     4741 cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa ccttgccgcc
     4801 gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg cacgatgggc
     4861 tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa atgggatggc
     4921 accgataccg tcaaggtcgg cagcgatgaa accctggttc gcgtgctgga cgaagaagtc
     4981 tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg gcaaattcgc
     5041 cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct catcaaggcg
     5101 gcaaaacaat gattaaacaa actattgtcg cgttgctttt gagtgtggga gcgtcatcgg
     5161 tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc aaaacgctaa
     5221 cgggcgcaga gcatttaatc gatctggtag ggcaacctcg actggcaaac agctggtggc
     5281 ctggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag cagcaggcgt
     5341 tgctgacacg gctggcagaa cagggcgcag attccagcac cgacgatgcc gccgccatta
     5401 acgccttacg ccagcaaatc caggcattga aagtgacagg caggcaaaaa atcaatcttg
     5461 atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc aactacacgc
     5521 tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc catcctggca
     5581 atcagccatt cactccaggt cgcgacgtgg cgagctatct ctctgaccag agcctgctca
     5641 gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg caaaaagcgc
     5701 cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt atttatgttg
     5761 gcctcgcaga ttccgtctgg agtgagacgc ctgatgccct taacgccgac attcttcaga
     5821 ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct tactggcgct
     5881 gggcattagc acagcttgct acggcgaaac atatcctgcg cccattggtc cgtcgcagtc
     5941 ggatttcggt ggcgtaggat tattacaaac tcccaccgcg cgtatggcac gggaagggga
     6001 gttgagtctg aactatcgcg ataacgatca gtaccgttat tactcagctt cagtgcaact
     6061 cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc agtacagcag
     6121 cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc tcaaactgcg
     6181 tttgtgggaa gagagttact ggctgccgca agtggcggtt ggtgcgcggg atatcggcgg
     6241 tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc cgttcgattt
     6301 tacgctcggc ctgggctggg gatatctggg caccagcggt aatgtaaaaa atccgctctg
     6361 ttcagccagt gataaatatt gctatcgcga taacagctac aaacaggcgg gatctatcga
     6421 cggtagccag atgttccacg gtcctgcctc actgtttggc ggcgtggaat accagacgcc
     6481 ctggcaaccg ctgcgtctga aactggagta tgaaggtaat aattatcagc aggattttgc
     6541 cgggaagctg gagcaaaaaa gtaagtttaa cgtcggggcg atttatcgcg ttaccgattg
     6601 ggccgacgtt aaccttagct atgaacgtgg caacaccttt atgtttggcg tcacgctgcg
     6661 caccaacttt aacgatctgc gcccgtccta catcgataac gcccgcccgc aatatcaacc
     6721 gcagccgcag gatgccattt tgcagcattc ggtagtggcg aatcagttaa cgttgctgaa
     6781 atacaacgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc tgtatgttac
     6841 cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc atccgtgcta atcggatcgt
     6901 gatgaacgat ctgccggatg ggatcaaaac gatccgcatt acggaaaatc gccttaacat
     6961 gccgcaagtg acgacggaaa ccgatgtcgc cagcctgaaa aatcatctcg ccggagagcc
     7021 gttgggccac gaaacgacgc tggcgcacaa acgcgtcgag ccagtggttc cgaagtccac
     7081 cgagcagggc tggtatatcg acaaatcacg ctttgatttc catatcgatc cggtgctgaa
     7141 ccagtcggtc ggtggcccgg aaaactttta catgtatcag ctgggcgtga tgggaacggc
     7201 agatttgtgg ctgacggacc atctactgac caccggcagc ctgtttgcaa atcttgccaa
     7261 caactacgac aagtttaact acaccaatcc accgcaggac tcgcacttac cgcgcgttcg
     7321 tacccatgtg cgcgagtatg tgcagaacga tgtctatgtg aataacctgc aagccaacta
     7381 cttccagcat ctgggcaacg gcttctacgg tcaggtctat ggaggttatc tcgaaaccat
     7441 gtttggcggt gcgggggccg aagtgttgta tcgtccgctg gacagtaact gggcgtttgg
     7501 tctggatgcc aactacgtta aacagcgcga ctggcgtagt gcaaaagata tgatgaaatt
     7561 caccgactac agcgtgaaaa ccggacatct gaccgcctac tggacgccat ctttcgccca
     7621 ggacgtgttg gtgaaagcca gcgtcgggca gtatctggca ggggataaag gcggcacgct
     7681 ggagatcgct aaacgctttg acagcggcgt ggtggtgggt ggctatgcca cgatcaccaa
     7741 cgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg tctcggtacc
     7801 gttggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct ggacgccgct
     7861 gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgaca tgaccagcga
     7921 ccgtagcgta aatttccgct aagtcatggg aaaggtgcca gttttcgcac tcactggcac
     7981 cctcattctt aatgacctgt tgagccatca ggaacgcagt tcacccccgg tttacgatct
     8041 ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg caggtctggt
     8101 tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag gtcggcttta
     8161 tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa catcaaagca
     8221 gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg ttccagagga
     8281 acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg gtgatttttg
     8341 aattaccggc tttggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg gcttatccgg
     8401 cctacgggta ggtatatccg gctttggtgg aggcgcgctc caaatccagg ttgaacaaaa
     8461 catacacaaa aaatatagat ctccgtcaca tttttgcgtt atacaggaag ctcgccactg
     8521 tgaaggaggt actgctatga cgtcactctc tcgtccgcgc gtggagttta tctccaccat
     8581 tttgcagacc gtactcaatc ttggcctgct gtgcctcggc ctgattttgg ttgtcttcct
     8641 cggcaaagaa acggtgcatc tggctgatgt gctgttcgcg ccagaacaaa ccagcaaata
     8701 tgagctggta gaaggactgg tggtttactt tctctatttc gaatttatcg cgctgattgt
     8761 gaagtacttt cagtccggtt ttcacttccc gctacgttac tttgtctata ttgggatcac
     8821 cgccattgtg cgcttgatca tcgtcgatca taaatcgcca ctggatgtgt tgatctactc
     8881 ggctgcgatc ctgttactgg tgatcaccct gtggctgtgt aattcgaaac ggctgaagcg
     8941 ggagtaaaaa gacagcacgc cgaaatggcg cggcgtgctg gacaggaaga ttacagcgta
     9001 gcagtttgtt gtgttttctt cgtttccggt tcccagagcg cttccagctc ctcaagggtt
     9061 ttacctttgg tttccgggac aaatttccac ataaacagtg ctgccagaac gcccatacaa
     9121 ccgtaaatcc agtaggagaa accgttgtgg aaatgggcca ccagccagga gtttttgtcc
     9181 atcatcggga aggtccagga gacgaagtag ttcgccagcc actgggccgc caccgcgatt
     9241 gccagcgctt taccacgaat agcattcggg aagatttccg acagcagtac ccagcatacc
     9301 ggaccccagg acatggcaaa ggcggcaaca tagaacagca tcgacagtag cgccacaata
     9361 cccgatgcct gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc cattccgagt
     9421 gcgccgataa tttgcagtgg cttacgacca aatttatcca cagtcataat tgccagtacg
     9481 gtaaaggtga ggttgataac tccgacaata atggtctgca acagcgcgat atccgtgctg
     9541 gcccccagcg ttttgaacac ttccggcgcg tagtacagca ccacattgat gccgacaaat
     9601 tgctggaaga tggagagcat aacgccgatt acaatcacgc ccacgccaaa catcagcaga
     9661 cgaccaccgg ttttgcggcc atgatccagg gagtgtttaa tttcctgtac tgcctgagtt
     9721 gcaagcgtgt tgcccataat tttgcgcagg atactttccg tctgttcttg cttgccgcgt
     9781 gacatcagcc agcgaggact ttctggcacg gtatacagca gcattaagaa cagcagtgca
     9841 gggatacatt ccgaggcaaa catataacgc ctgccgtcag tattcagcca gctggcatca
     9901 ccggaacggg caataaaata gtttacgcag taaactaaaa gttgcccgaa aataatcgca
     9961 aactggttaa atgaaaccag ttttccgcga atatgagctg gagccagttc cgcaatatac
    10021 attggtgaaa gcattgaggc taaaccaacg ccaataccgc caataatacg ataaataaca
    10081 aattccggga cataacctgc cagataaata ggtactgtgt tgtccgggtt tatagaggta
    10141 aaaccaagtt ctggccaggc agaacctaca ccagaaataa aaaacaggac agcagaaatc
    10201 ttaagtgaat cacgacggcc gaagcggtta ctgcaataac caccgagggc accgccgatg
    10261 atgcaaccaa tcagagcgct ggctacgcaa aatcctaaca gggagttggc agcggattca
    10321 cttaagtttt gtggagcaac aaagacggta ttgagtgact caacagtacc ggaaataacg
    10381 gcggtgtcgt agccaaataa taaaccacct aatgtagcga ctaaggtaat cgaaaatata
    10441 taactggaat tatactgggt attcattcag acctgcctta gaccattctg atcttttatc
    10501 agtgcattga tgttggaatt gtcaggcagt taaggcaatt atcatttttt acaatgcgtt
    10561 tacgtaatgt cttttctgtg atcttaattg tgataattat caaaaattga tgaaaacaat
    10621 actattgccg tgactcagag cacgaaagag aattatcgta agtgggaaaa caaataacgt
    10681 aaaaataata agttctatga tgggaatatt aaccggcgaa cgattcagat tgcagacgaa
    10741 agaaaaaa
//
LOCUS       DAECNO010000123        10688 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.123, whole genome shotgun sequence.
ACCESSION   DAECNO010000123 DAECNO010000000
VERSION     DAECNO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.123"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..213)
                     /locus_tag="KKO08_003454"
     CDS             complement(<1..213)
                     /locus_tag="KKO08_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBD6319569.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSS"
     gene            704..835
                     /locus_tag="KKO08_003455"
     CDS             704..835
                     /locus_tag="KKO08_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBD6319570.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            1095..2090
                     /gene="pgl"
                     /locus_tag="KKO08_003456"
     CDS             1095..2090
                     /gene="pgl"
                     /locus_tag="KKO08_003456"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBD6319571.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2131..3084)
                     /locus_tag="KKO08_003457"
                     /pseudo
     CDS             complement(2131..3084)
                     /locus_tag="KKO08_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3268..4320
                     /locus_tag="KKO08_003458"
     CDS             3268..4320
                     /locus_tag="KKO08_003458"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBD6319572.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4396..5829
                     /locus_tag="KKO08_003459"
     CDS             4396..5829
                     /locus_tag="KKO08_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBD6319573.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            6012..8192
                     /locus_tag="KKO08_003460"
     CDS             6012..8192
                     /locus_tag="KKO08_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBD6319574.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8333..9616)
                     /locus_tag="KKO08_003461"
     CDS             complement(8333..9616)
                     /locus_tag="KKO08_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBD6319575.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9751..10632)
                     /locus_tag="KKO08_003462"
     CDS             complement(9751..10632)
                     /locus_tag="KKO08_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6319576.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2624 a         2462 c         2663 g         2939 t
ORIGIN      
        1 cgaggaaagc gccaaggctc gcaggtccag tttatgcccc ttagaggtaa gacatgcacg
       61 aagtcttctt accgcctcag tacgctgttc ttccggtaac ccctgtttct cccaatcatc
      121 ccattcggac aaataatggt tgggggatgt aattgtgctg atatgtggaa ataaacagga
      181 tgaaatgttt ctgagcatat taatttctcg catatggata tggaattaga ttgtgaataa
      241 attgtattta atgtatctct cctaatgagg agggtgtttc agaatattcg ccataaggga
      301 atgagtgtta tatcatgctt tctaccctgg taagtatatt tgcttttttc gtatatttgg
      361 ttcatctttt tttgatgttt tacatattca caatgcagag gatttatacc agaggacatt
      421 agtcttgagt aagatactac aacatctcca actattttag ccagtctgac tacttgactt
      481 ggtaatattt gttgctaata atcatgtata aaataagctt atatacatag taacttcagc
      541 aaaggattta gtaatgatca aaatcatatc tcaatgtact gtttttttct ttaaaaaaac
      601 agtacattct attttataat gactgtgtat tcccatggaa ggagatcacc caattctccc
      661 ccccgacttt tgacgcagtg tcttgttaac agggggattg accatgctta ttggctatgt
      721 acgcgtatca acaaatgacc agaataccga tctacaacgt aatgcgttta tttgtgccgg
      781 aagtgagttg attgttgcgc gaatgggaag ttggccagaa gttgttatgg catagtgttt
      841 acggtcgttg atacaggtaa acaaaagcgg ggagagtcat actccctgaa tagtcgcgaa
      901 aatatctgtg gcattgtcaa attcgtgtcg gtgactaata ttttagtaat atgccattcg
      961 aaatggagtg ctaacttatt gatttaattg atgtgcgata ttgtatttta tcgtgttttg
     1021 ttgtctgttt tttatccagt aagtgttgtt atatgtatga aagataaggg atttatcatt
     1081 tttgaggtgt gttcatgaag caaacagttt atatcgccag ccctgagagc cagcaaattc
     1141 acgtctggaa cctgaatcat gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg
     1201 ggcaggtgca gccgatggtg gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc
     1261 ctgagtttcg cgtcctggcg tatcgtattg ccccggacga tggcgcactg acctttgccg
     1321 cagagtctgt gctgccgggt agtccgacgc atatttccac cgatcaccag gggcagtttg
     1381 tctttgtagg ttcttacaat gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc
     1441 cagtgggcgt cgtcgatgta gtcgaggggc tggacggttg ccattccgcc aatatctcac
     1501 cggacaaccg tacgctgtgg gttccggcat taaagcagga tcgcatttgc ctgtttacgg
     1561 tcagcgatga tggtcatctg gtggcgcagg accctgcgga agtgactacc gttgaagggg
     1621 ccggcccgcg tcatatggta ttccatccaa acgaacaata tgcgtattgc gtcaatgagt
     1681 taaacagctc agtggatgtc tgggaactga aagatccgca cggcaatatc gaatgtgtcc
     1741 agacgctgga tatgatgccg gaaaacttct ccgacacccg ttgggcggct gatattcata
     1801 tcaccccgga tggtcgccat ttatacgcct gcgaccgtac cgccagtctg attaccgttt
     1861 tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg cttccagcca acggaaaccc
     1921 agccgcgcgg cttcaatgtt gattacagcg gcaagtatct gattgccgct gggcaaaaat
     1981 ctcaccacat ctcggtatac gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc
     2041 gctatgcggt cgggcaggga ccaatgtggg tggtggttaa cgcacactaa ccgctgattt
     2101 aaccggcgca gtctctcctg cgccggtgta ttaacttatc tcctgtaacg catgtctctg
     2161 gcgttcgacg atattggtcc acaaattgtc tttatcgtca gtccacaaat taatcagcaa
     2221 ggaaaaaaag cgttcagctg ccggagaaag cacggcatct ttgcggcgaa taatccccaa
     2281 tgtccgacgt ataacgggtt ccaccagtgg gataccaata atcgaagaat agggcgcatg
     2341 gggcattgcc agaccaggaa gcgccgaaat ccccagtcct gcctccacta gtcctaatga
     2401 cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc cagggcttgt cggctagctg
     2461 ttgttctatc aacaatcggt tgccgctgga ggaacgtacg ccaatcattt tgtaacccac
     2521 taactcctgc cattctacaa gctacttttt ggccaatgga tggtcacgcc gacaggccag
     2581 cacgaatggc tcgttgacca gtggggtaaa atcaatggat gaatttgtga cgttgttcat
     2641 gtttatgcca aagtctgatt cgttacacag caccgattcc atgcaattat tcgtgccttg
     2701 ttccagaatc cgcactttaa tattgggata cagctcatta aatttaccga ttgccagcgg
     2761 taaaaaataa aatactgcgg tcggaataca cgctaatgtc accatatcac gatgataagc
     2821 attcatatca cgaatattaa aaagcgtttc atcgaatttc tttattaatt cccgcgcctc
     2881 cgggagcaac ctttttcctg cttttgttaa cgtaaccttg cgagttgtac gctcaaatag
     2941 ttgaatatgt aaatcttcct ccatcttttt tattcttcgc gttaatgcag gttgcgtaat
     3001 attgagtaat ttagcggcat tgttaaatga actggactct gccagtatga caaatgcctt
     3061 catacttgat aattcatgct tcattattac tccggaaaat ggaagcgacg attttggggg
     3121 gctggtcgtt aaaaatttta actgcattta gccaacttaa attaatgaaa aaatgttatt
     3181 aatcgttgag ctaaagtcat tagagatgct ttgtccttaa tgtaaccata tcgcaataag
     3241 ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat gatgcgaggt
     3301 ggaacctcga gggccgcgtt cctgttagcg gaacatttac ccgaagatca aacgcagcgc
     3361 gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga cggtattggc
     3421 ggcggtaatc cactgaccag taaagtcgcc attattagcc gttccagcga tccgcgtgct
     3481 gatgtcgatt atctgtttgc tcaagttatc gtccatgagc aacgtgtcga taccacgcct
     3541 aactgcggca atatgctgtc tggcgtaggg gcattcgcca ttgaaaatgg tttgattgca
     3601 gcgacttcgc cagttacccg tgtacgtatc cgcaacgtca atacgggtac gttcatcgaa
     3661 gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagcgccag aattgacggc
     3721 gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg aacgaaaacc
     3781 ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc ggtgacctgt
     3841 atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa aacaggttat
     3901 gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga atctatccgg
     3961 ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat ccctaaacct
     4021 gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat
     4081 tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag ttgtgcattg
     4141 gaaggcaccg tcacccgaca aatcgtccct tctgtaggat atggcaatat caatattgaa
     4201 caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta
     4261 cgcgcatctg ttattcggac aaccagaaaa atattttccg gtgaagttta tcttccctga
     4321 aaaaattcgt tgtcaggata aggacaatca ataaaggact tctgtatgag tcatacagaa
     4381 agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat attagcgatc
     4441 ccatgtatta ttggttttat gccagctccg gcaggattaa gcgaactggc gtgggtgctt
     4501 tttggtattt acctggcggc cattgtgggt ctggttatca agcctttccc ggaacctgtc
     4561 gtactgttaa ttgccgttgc tgcctcaatg gtggtggtcg gtaacttatc cgacggtgcg
     4621 tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg
     4681 gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacttat tgcctatctg
     4741 ctgattggta aaatcggtaa caccatgctg ggtctgggtt acgttacggt attcctcgat
     4801 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggcat tgtgttaccg
     4861 atcatcaaca gcgtggcgat ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc
     4921 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
     4981 accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
     5041 attagctggg gtggatgggc gctggccgcc ggattgccgg gcatcattat gctgctggtc
     5101 accccgctgg taatttacac catgtatcca ccagaaatta aaaaggtgga taacaaaacc
     5161 atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
     5221 ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa
     5281 tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa
     5341 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcggc
     5401 ttaagctcct tattatcgaa agttaaattt ttcgaatggt tagctgaagt ctttaaaaat
     5461 aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatc
     5521 atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt acctgtattt
     5581 gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
     5641 tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
     5701 ggcgtgggtt acaacgatat taaatcctgg tggttggtcg gtgcggtact gacgatatta
     5761 accttcttgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
     5821 atgctgtaaa tatactcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     5881 ccagtcactt acttatgtaa gctcctgggg attcactcac ttgtcgcctt cctgcaactc
     5941 gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg cgcagatatc
     6001 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
     6061 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagcaaaa aaaggcacta
     6121 ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa cttaaaatta
     6181 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
     6241 cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
     6301 gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg gcagctcagc
     6361 gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat atgcgtgaga
     6421 tgatggctag cgggggcaaa atgatcctcg ggtcagacag ccacacccgt tacggtgcat
     6481 tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg cttaatgaca
     6541 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
     6601 atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa aacggttacg
     6661 tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct accgatttcc
     6721 gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc tggcaaactg
     6781 atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac tgccagctta
     6841 accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
     6901 caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca ctgaaccaga
     6961 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
     7021 ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag caggggatta
     7081 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
     7141 gtcaatcctg tggcaatgac accttctcgc tggcggttta cccgtcatca cagccggtgt
     7201 ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc gcaatcatca
     7261 gaaccgcatt ctgtggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
     7321 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gccaatgggc
     7381 agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg gcaaacggtg
     7441 gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag tacgccttcg
     7501 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca actcagcaac
     7561 cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg actgacaata
     7621 tcgtcctgaa agtgtgctcg aagatcctcg acgaagtcac caccaccgac gaactgattc
     7681 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcgggg aatgtcagcg
     7741 agctgacaga ggtgtttgcg cgcattaagc agattgctgg tcaggagcat attgatccgc
     7801 tgcaaactga aattggcagc atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg
     7861 aacaggcggc gagctgccag cgtgtgattg gcggtctggc gaatattgcc gaggagtacg
     7921 cgactaaacg ctaccgttct aacgtcatca actgggggat gttaccgctg cagatggcgg
     7981 aagtgccaac ctttgaagtg ggggattaca tttacatccc tggcattaaa gcggcgctgg
     8041 ataatccggg tacgacgttt aaaggttatg tgatccatga agatgcgccg gtaacggaaa
     8101 ttacgcttta catggaaagc ctgacggctg aagagcgcga gattatcaag gcgggtagtt
     8161 tgattaactt caataaaaac cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt
     8221 cggcacttat tgtcggatgc gatgctggcg catcttatcc gacctacaaa tcgcatcgaa
     8281 ccgtaggccg gataaggcgt ttacgccgca tccggcaaat agttaatagc tcttacttct
     8341 tcgcctctgc aaccactttg ctgcccacgc cgcggttatt gtattcccac atgcggttgt
     8401 agttagtgtc attcagattg cgctgtattt cgtcgttatc atctacgctg ccggtattac
     8461 ccgcaaacgg acgattagag atcaccgcat cggcccacgg tttagccgtg ttaaaacctt
     8521 cgttgatggc gctatcacgg atcaccacct gaccgttggt attagcatca acatccagcg
     8581 agcggcccag ttgcgccacg ccatcaccgg aagcattgaa acggctgttt acggcgagga
     8641 aaccgtaata gatgttagac agcgtagccg gtgcaaacac atatgcttct tgctgggtac
     8701 gtgagttcac cacgcggaat tcggtgttat cgaacaccac tgcgccgcga ccagaaacga
     8761 tatccacatc cccttcaata tagctgttgg tcaccagcgt acgcggctga cggttggttt
     8821 ccagacggtt ctgcacaccg ctgttggtga caaagaaggt gttctgacga ccgagaatgt
     8881 taacgttgtt aatctgtact tggtcaccgt cagtacgcag tgctaccgcc ggatggttac
     8941 ctgcatctac gctatcgccc agcgtgtttt cgatggtcag attttgcagt tgcagtccat
     9001 tgttttgtga ccagaagacc gcagaacaca gaacaccgat actgtcgctg cgtttgctct
     9061 ggcagctatc gtacatatac cacgctggtt tacctggcat atatttgccg cgcgggttga
     9121 cgtcgtgacg ccagtcggca gggctcatgc ccccatcaag ggaaagccca atcttcacat
     9181 caatcggttt ttcacctgta ccgtacagag tgattccacc tggagcagca gggacataca
     9241 ccgttccctg atactcccca ggcatcacgg caatatactg gcgcttgttg gtgcgcttga
     9301 taattgccgc atctaccgcc gcctgaatcg tggtatgcgt tacaccttga gtacccgccg
     9361 ggccgacaac aaagtcaggt tgcgcaggca gggttatcgg ggacggattc cacgctgcag
     9421 cacctggtgt cagggatgca aaatagtgtt gcgcatcgaa attcagcgct tcttttgccg
     9481 acagaatcgg gcgagaagag gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg
     9541 gtgttgagct acaggcggtc agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa
     9601 ctgaaaatgt gttcacaggt tgctccgggc tatgaaatag aaaaatgaat ccgttgaagc
     9661 ctgctttttt atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg
     9721 aacaggtcat tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactccct
     9781 gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttgtgcccga gaagatgttg
     9841 agcaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc gcaactcgtg
     9901 aaaggtaggt ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc gcataaaata
     9961 ccttgatact gtgccggatg aaagcggttc acgacgagta gatgcaatta tggtttctcc
    10021 gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatatcccga gagcatcaac
    10081 atgcaatgtt gtagggatgg caatttttac gcctgttttg ctttgctcga cataaagata
    10141 tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgtt gcccggtaac
    10201 aacagccagt tccattgcaa gtctgagcca acatggtgat gattctgctg cttgataaat
    10261 tttcaggtat tcgtcagccg taagtcttga tctccttacc tctgattttg ctgcgcgagt
    10321 ggcagcgacc gggtttgttg ttatatggcc ttcagctatt gcctctcgga atgcatcgct
    10381 cagtgttgat ctgattaact tggctgatgc cgccttgccc tcgtctatgt atccattgag
    10441 cattgccgca atttcttttg tggtgatgtc ttcaagtgga gcatcaggca gccccctcct
    10501 tattgcttta attttgctca tgtaatttat gagtgtcttc tgcttgattc ctctgctggc
    10561 gaggattttt tcgtagcgat caagccatga atgtaacgta acagaattat cactgttgat
    10621 tctcgctgtc agaggcttgt gtttgtgtcc tgaaaataac tcaatgttgg cctgtatagc
    10681 ttcagtga
//
LOCUS       DAECNO010000124        10124 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.124, whole genome shotgun sequence.
ACCESSION   DAECNO010000124 DAECNO010000000
VERSION     DAECNO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.124"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..177)
                     /locus_tag="KKO08_003463"
     CDS             complement(<1..177)
                     /locus_tag="KKO08_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD6319577.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
     gene            complement(380..847)
                     /locus_tag="KKO08_003464"
     CDS             complement(380..847)
                     /locus_tag="KKO08_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD6319578.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRTQCLK"
     gene            complement(844..1377)
                     /locus_tag="KKO08_003465"
     CDS             complement(844..1377)
                     /locus_tag="KKO08_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBD6319579.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(1443..2072)
                     /locus_tag="KKO08_003466"
     CDS             complement(1443..2072)
                     /locus_tag="KKO08_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319580.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(2076..2291)
                     /locus_tag="KKO08_003467"
     CDS             complement(2076..2291)
                     /locus_tag="KKO08_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD6319581.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(2584..2658)
                     /locus_tag="KKO08_003468"
     tRNA            complement(2584..2658)
                     /locus_tag="KKO08_003468"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2623..2625),aa:Gly,seq:tcc)
     gene            complement(2662..2737)
                     /locus_tag="KKO08_003469"
     tRNA            complement(2662..2737)
                     /locus_tag="KKO08_003469"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2702..2704),aa:Thr,seq:tgt)
     gene            complement(2740..2816)
                     /locus_tag="KKO08_003470"
     tRNA            complement(2740..2816)
                     /locus_tag="KKO08_003470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2780..2782),aa:Arg,seq:tct)
     gene            complement(2818..2894)
                     /locus_tag="KKO08_003471"
     tRNA            complement(2818..2894)
                     /locus_tag="KKO08_003471"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2858..2860),aa:Ile,seq:cat)
     gene            complement(3094..3474)
                     /locus_tag="KKO08_003472"
     CDS             complement(3094..3474)
                     /locus_tag="KKO08_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD6319582.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(3467..3667)
                     /locus_tag="KKO08_003473"
     CDS             complement(3467..3667)
                     /locus_tag="KKO08_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBD6319583.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(3762..4019)
                     /locus_tag="KKO08_003474"
     CDS             complement(3762..4019)
                     /locus_tag="KKO08_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319584.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(4016..5407)
                     /locus_tag="KKO08_003475"
     CDS             complement(4016..5407)
                     /locus_tag="KKO08_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBD6319585.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(5404..6282)
                     /locus_tag="KKO08_003476"
     CDS             complement(5404..6282)
                     /locus_tag="KKO08_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD6319586.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(6293..7201)
                     /locus_tag="KKO08_003477"
     CDS             complement(6293..7201)
                     /locus_tag="KKO08_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD6319587.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(7188..7421)
                     /locus_tag="KKO08_003478"
     CDS             complement(7188..7421)
                     /locus_tag="KKO08_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319588.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(7418..8434)
                     /locus_tag="KKO08_003479"
                     /pseudo
     CDS             complement(7418..8434)
                     /locus_tag="KKO08_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            8366..8629
                     /locus_tag="KKO08_003480"
     CDS             8366..8629
                     /locus_tag="KKO08_003480"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBD6319589.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            8619..8927
                     /locus_tag="KKO08_003481"
     CDS             8619..8927
                     /locus_tag="KKO08_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6319590.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(8969..9145)
                     /locus_tag="KKO08_003482"
     CDS             complement(8969..9145)
                     /locus_tag="KKO08_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HBD6319591.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(9142..9552)
                     /locus_tag="KKO08_003483"
     CDS             complement(9142..9552)
                     /locus_tag="KKO08_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HBD6319592.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(9549..9827)
                     /locus_tag="KKO08_003484"
     CDS             complement(9549..9827)
                     /locus_tag="KKO08_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319593.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(9902..>10124)
                     /locus_tag="KKO08_003485"
     CDS             complement(9902..>10124)
                     /locus_tag="KKO08_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319594.1"
                     /translation="SGGQIQCATSPASVLMLEKQRNGEGWEGGVPLFLDEQSHQFLQL
                     ESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         2449 a         2715 c         2324 g         2636 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg
      301 atgcgcggtt tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact
      361 ttgtgatttg caaaaaaatt tatttcaggc actgagtcct gatgtactcc tgaagcattc
      421 tcaatgctgt ctggtcgctg atgattccgt cccggatacc gagaacgttt cgtccagcaa
      481 ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag gtttcggctg
      541 aggatggcac agggcatttt cctttgacga gcacccgacc accattatca agcttgcgcc
      601 gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta gcatcgagtg
      661 catcagcatc acgctggcgc tgctgcatgt cagtaatggt tgcgttcgcc agcttcagtt
      721 ctccggcgtt tttgtcgcgc tgctctttgt aggtaatggc gttatcacgg taatgattaa
      781 cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggtgactc
      841 tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg
      901 tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg
      961 aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa acgtcgacgg
     1021 gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg
     1081 ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat
     1141 agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc
     1201 atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt
     1261 tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc
     1321 tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga
     1381 tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata
     1441 agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca
     1501 cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc
     1561 tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt
     1621 tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc
     1681 cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa
     1741 ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct
     1801 tcctctccgg caccgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt
     1861 tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg
     1921 actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct
     1981 tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga
     2041 ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc
     2101 ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga
     2161 cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa
     2221 aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc
     2281 atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg
     2341 aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa
     2401 gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg
     2461 atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaag gcgatcattc
     2521 ataacttaaa cgtctttcag tcactccggg atttcccatc atcgcagact gaaagactct
     2581 gactggagcg ggcagcggga atcgaacccg catcatcagc ttggaaggct gaggtaatag
     2641 ccattatacg atgcccgcat atggtgccga ctaccggaat cgaactggtg acctactgat
     2701 tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac cgaggactcg
     2761 aacctcgcac cgtcaactta gaaggttgat gctctatccg gatgagctag tggtggttgg
     2821 tggcccttgc tggacttgaa ccagcgacct ggcgattatg agtcgctcgc tctgaccaac
     2881 tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc aaactcatct
     2941 gaccaccagc gtgtttaacg tcctgtaccg tttttcaggc ataaaaaacc cgcccggagg
     3001 cgggtttaag ctgtgtggcg aagcaaccac tcttaacaga atatcccgtt ttttacgtac
     3061 gtaaaatatt ttctagaaag tcgcccctta ccatcaggga tgttcaatat atttgtccat
     3121 ttctaaccgg acacccaaca tcatcaacat tccttccacc accccctcgg ctttttgtag
     3181 cttctttcct atataaccat ctgagcatcc atgcttccgc gccagcgtca tgaatgtcat
     3241 cccaaacacg tagtagtcaa ccagcaagtc atgtagatcg ttgttgttcc ggttaaggcg
     3301 ggccatacac ccacatataa ccatcgcgtc atcgtcacag cattgcgggc gggattttac
     3361 ttttgaaggg attaatccct taaaaccggc agcaatggac gaccaggtca catcctcatg
     3421 attattagcc acccacgcgc cccagcgctc aagaacctgc tgaatatcac gcatcactgt
     3481 ctttacccct gtcccatcca cgatgaacaa tcagaacacc atcaacaatg gcgtgccctt
     3541 tgccctcttt atcttcagca tattttctta ccgtggcgcg attacagttc agcattcgtg
     3601 aaacttcggt catattacct cgtgtctgga taagcagttc cggtatcgtt tgaattttga
     3661 cgctcatcaa atactctccg gttcggtgat ttttatcccg ggctttccac cagggacata
     3721 atgatcgcgc acaagccagg gcaaaacaaa ctcatggcgc atcagcgcag cgcctcctgc
     3781 accagttttt caaactttcc gactctggtt tccagctctg ccacacagtc caccagctca
     3841 tctactgctt tctgtgcgcg atgcttcgcc tgcatcagtt cccgaagcgc gggtaccata
     3901 tctttacgga tagcatcttt tgttatgccc gttttttcga gttgttcagc atgacgcagc
     3961 atttcctgcg cgtgtttacg caattgttca ggggtaaaag tcattgtctg gttgttcaaa
     4021 agaaacgctc catcttactg ctgtcagttc gtttattact gtatctgcgc ggattgccgg
     4081 gcttcatggg agtggaaagc acccgtgcac tttcctggtc cacaggcaga aaatgtccgt
     4141 tataaaaacg ccggtaaatc gttcccagag aaccgttacg ttgtttcgtg atattgattt
     4201 ctgcgatgcc cctggcctgc gtatccgggt tgtacacttc atccctgtaa agcatcagaa
     4261 tgatgtctgc atccgcctct atttctccgg aatttttcag gtctgagttc atgggacgtt
     4321 tattgggtct ggactccaca ccgcgggaga gctggctcag cgcaatcaac ggaaaaccac
     4381 cggattttgc caggccttta agcccctttg agatttcacc cacggcaagg tcatgacgcc
     4441 ccgtggttcg ggtttttatc agcccgagat aatcaaccac caccagcgcc gtttccggat
     4501 atttaatcag gtggtgtttc gttgttgcgc atatctcatc aatggccagg ttcgcctggt
     4561 ccaccatcca gatattgcgc ccggtcatcc gccccacccc ttgtgagaaa cgcgtccagt
     4621 cttcatcttc aaagtgagcc acagatttca ggcgtgatac tggcatccct ccagccgcag
     4681 acaccatacg ttcaccaatc tggatgttcg ccatctccat ggtgaacaga agcacaccat
     4741 gcccctgctc agtcaccttg tcgatgatgt ccagcgcaag ttcggttttc cccatcgaag
     4801 gacgggcggc aatgaatacc aggtctccgg gctccatacc gcctgttttt gcgtccagtt
     4861 catcaatacc ggtcatcaac gtcctggatt tctccagccc ctgattccgg cattcaacac
     4921 gctcaaccac ttccggaagc acatcatcaa tatgtaccgg ctgaatgacg ccctttcctg
     4981 tcgacagtgt gaccatcatg ttctgcgcat ccttcagggc atcttcagct gcttcacagg
     5041 tatgcgcatc acgtaatttc tgcagcgcct cattcagtgt tttttctgca tcgcgcagtg
     5101 cggcattgcg ccgcaacgct gcaacatagt gctccagtga agacttcacc caggttttac
     5161 gcccggtatc agtaatcacc ggggcaagtt ccggcatctc attacacaac agcacggggt
     5221 caatcactcc tgaaacacgg gcctgtctgc agatgcctgt atagatatcc tgatacgctc
     5281 gtacagaaaa aacgtccgct ggcagtgtgg ccagaatatc catcacttca ggatctgctc
     5341 cgcgcagaaa gaacgcgccg atgacagcgc cttcaaggtc atcgttacgc catactggag
     5401 ttgtcatgca gccacacctc tgatacgaga acggtaactg ggccagttaa acgacaacca
     5461 gttgcgtccc ccgtctgtga tcctgtcggc aattcgggga ctgatgaacg cccacaactc
     5521 ttccggtgag aggttactga taagaatggt gggcaagata ctttcgtacc tggcgttgat
     5581 aatttcctgc aaaatagcca tttcagccgc gctgccaaac tgaacgccaa cttcgtcgat
     5641 gatcagcaaa tccattgacg cataacgctc aataacttca tccgctgttt tttcgctgtc
     5701 attccgccag caatttttca cagcacgggt aaggcgcatc acgtcggtga tctccacact
     5761 ggccagatag ttacggatga tgtgttttgc catagccaca gccagatgat tttttccggt
     5821 accacaactg ccggtcataa caagactggt accgttctcc agcatatctg gccagttctc
     5881 cgcatagcgg cgacaggccg caagatttct ggctgcgcca ggattaacct ccagataatt
     5941 atcaaactcg cagtcccgaa aacgcagggc aattccggcg ttatcagtca gctcttccgc
     6001 cttgatggac gacagctcca tggtcaaatc gttggcctca gcgattaagc agtcagggca
     6061 gcatgaaatt tcttctctgt cctcgccatt acgatcgctc cacaccagta tatgcgtgtg
     6121 atattcgcca tgtttttcgc aatatccgcg accttcacgc atcaggcagg aacgataagg
     6181 ccatggcttt tcgcccttct gagcaaatgc aatctctgcc cgtaactcat ccattcgcgc
     6241 ctgtagtctt ttttgttgtt cacgcaggtt aaacgtcatc atcgctgtca cctcagaatg
     6301 tcagtttgtc actggattta ccgaatttgt cagacatggc tcccaggcca gccaggacat
     6361 cgacctgtcg ctgtcgccca cctctgggag cggctggctg ttgccagaag tcttcgaagt
     6421 gacgatcggg tccaaagaac gtcgcagcct gcttcacgaa ctgtgtgccg gtatttcctg
     6481 tggcacgtac ccaggcagca tagcgtttca cgccatcaag catggcttca ggttttattc
     6541 cctccctgag ccgggctttc caggctttga aggctgcgga ttttgagtta ccaccagcac
     6601 gttttggata ttcctgccag gcctgttcaa attccggtga atattcctgt cgggaagaac
     6661 gcgctggtgc agacgcgtca gcggatgcgc caatagtgtt tttactctct gtagtattct
     6721 ctgaagtaat ctctgttgta ttctctgtaa gatcgaaatt ggttttccct tctccgcggc
     6781 gaggggtttc ccgtgtccgc ggtgaaggct ttccctcctc cgcgaaattg ggttttacag
     6841 tttcccgaaa acgggtttcc ccatttcggg aaaactgatt gttttcattg ataatttcat
     6901 taaggcgctc acaatctata cggtagaaca ttttgtgctc aagacgcttg ttggtttcaa
     6961 ccaaaatgcc tctggacaca agatgcttac gcgctacagc ctgttgttca aatgtaagtc
     7021 cggtttcgtg ttgtatctct tcacgcgttt tatgtacgcc ttccgctgca tgtgctttat
     7081 cctgccagta aaaaatctga ccaaagaaaa taacagcgtg cggacttccc atgtatttaa
     7141 cgagcccagg gtaataagca accggatgtc caaaatcgag cagaagatca gacggacgca
     7201 tagccacctc ccaggcgttt aaacattttt ccgggctgaa acgccaccag cggataactc
     7261 agggtatgag tacgtccctg aacctggcag acaaccttct ggctttcggt actgaccagg
     7321 caaacccgca gaacgtagcc gttgctggtg gtgaaccact gccccacacg ggggcaatgg
     7381 ttgtatcggt gatacaggaa attaacgatg tggcggatca tgggcgcacc tccttgtcag
     7441 aaccattcag cctggaatca acaagtgcag caccaaaaac agcatcacct acacggtcgt
     7501 acagtttgcc agccagcgga gattcaacgg ccttaagcat tgggtaaagc tggcttgtcc
     7561 agatttgatg gatttcacgc aaatgcaggt atacgcctct ggcgttttgt gcgacagatg
     7621 acatgtcagc cgcaccagct cctgataagc tcctctccat ctggttaaag gcattgatgt
     7681 atgcctcttt gaaccgggcg gcacgtttac cagtgaagcc catagcaagg aaggcgaagc
     7741 cgccgcgggt aatttggtag cagggaagtt tgcgagtacc gccgttgggc tggcgtacca
     7801 aaattgatgt ctccgcaaaa ttgcgggcac aaaactctgg agaacaatcc aaaatgcgga
     7861 tctttttcag aacatcgtca tgacgtttag agaagaagtt ggcaacagcc agggatgaag
     7921 taacagccta accatcaacg atggcaattt caggttgaga gagggttggg agagtagtca
     7981 tggtgacagc ccctatgttg aattcaaaga actcaccaca tgggacgcca atcacagagg
     8041 tggtgagacg tacagggttg gcgtaaccgg tcaacataga acccggcgca tcttgcgatg
     8101 tccctgcacg ccccaccata atttgggcgt agcaatgctc atgacacgaa aaaaccgcat
     8161 gagcgcggtt atgctctata ttgaatttca ggacgccaat cccggccccc gctttataag
     8221 gtgcagagac agtgtaacgt cccgaaattg cagaatcaat atattgtgca gatataacga
     8281 ttttgttatg ttttgctgta tgaactacac tatcgaatac tacagtgaag aggtccgact
     8341 ggaggtcgat cagcttccat tgagtatgcg tgccagatac cagcatcttg ttgaacgcat
     8401 gaaggtatac ggcagcaatc tcggagaacc tcacaccagc gcctttggtg acgggctttt
     8461 cgaactcaga attaaaggca acgatgggat cgcgcgtgtt ttttactgca ctctgacagg
     8521 gaaacgcatc atcatgctgc atagttttgt aaagaaagcg cagaaaacac cgccagccga
     8581 acgcaagaaa gctgaaacca gaatgaagga ggttaagcat gactggtaaa cgcactctcc
     8641 ccaccatgac acacgacgaa atggcagcca aatggatgga agacccggct tttaaagcag
     8701 aatacgacgc tatcgctgac gaattcgcac tgcttgatga aatgctggca gcacgcaaag
     8761 aagctggctt aactcaggct gaagttgccg agcgaatggg aacaaaagcg accgcgatca
     8821 ccagaatgga aagtaatctc gcatcaggta tcagcggccc atcatttgcc acactaaaaa
     8881 aattcgcccg cgctactgga aaaaaactcc agatccgctt cgtttaacca caccgcgccg
     8941 tcattctggc ggcgcaccgg ataaatagtc atttcctcgc acgatgtctt agccaccgga
     9001 tatcccacag gtgagtcgtg tagttgaagg tttttacgtc agattctttt gggattggct
     9061 tgcgtttatt tctggagcgt ttcgttggaa ggtatttgca gttttcgcag atgatgtcgg
     9121 tgaaacttcg tcgatgtcgc ctcattcata cctcctgtcg gtaaatctga caccctgacc
     9181 aatagcccag gctgttgtgt actcgatcag acttgccatg cgcttcacgc tcatctgcgc
     9241 gctgctttcg cgaatgttga cgcattctcc ttcaaggccg ggcaaaacat cagcttcctg
     9301 ttttgttgcc actgcatgac cgctgatcaa caaaaccttc cattgttctg gttttaacca
     9361 tttgccgcac cattgaacct gacgagcgat atccgccagc atcgcgtgaa attttgcgtt
     9421 ctggtcaaga tttcgcttgt agtcagtaat gcggatggtg actggcttgt ctttatcgag
     9481 aggagttgag aggatggcat ttattgcggc ttgctgttgt tgcttagttc ggaggaagat
     9541 tgtttgcttc atcgaaaatt cttctcttta attccagcgg ctctgatagc tttcattact
     9601 gcaattaccg ttttgtcctt cccatcctca tgccccatcg cataagcacc ttcttcacca
     9661 tctttccaaa agtcgtcatt cgattcgggc cagtcgatat ccagttcaat agctgctcgc
     9721 gatgcctgcc atatcaccca ggcaaactct tttaattcat cgtctcctgt gaactggctt
     9781 ttgtcttttg accaccagtt ttcaaactgt cggtagctat cgttcacttc cctctccccc
     9841 aaataaaaag gcctgcgatt accagcaggc ctgctattag ctcagtgatg tagatggtca
     9901 tttaatactc cgtcacgttt tcctgtcgcc acgcctcgtc atattccgat ttcggcatat
     9961 tggcgatgta gctatatggc gatcctgatt caagttgcag gaactggtgc gattgctcgt
    10021 caaggaacaa cggaacacca ccttcccaac cttcgccgtt acgttgtttt tcaagcatca
    10081 aaacagatgc cggtgaagtg gcacactgaa tttggccacc tgaa
//
LOCUS       DAECNO010000125         9951 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.125, whole genome shotgun sequence.
ACCESSION   DAECNO010000125 DAECNO010000000
VERSION     DAECNO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.125"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="KKO08_003486"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="KKO08_003486"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBD6319595.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="KKO08_003487"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="KKO08_003487"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBD6319596.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="KKO08_003488"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="KKO08_003488"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBD6319597.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="KKO08_003489"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="KKO08_003489"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBD6319598.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="KKO08_003490"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="KKO08_003490"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBD6319599.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="KKO08_003491"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="KKO08_003491"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBD6319600.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="KKO08_003492"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="KKO08_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="KKO08_003493"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="KKO08_003493"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBD6319601.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="KKO08_003494"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="KKO08_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBD6319602.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..>9951)
                     /locus_tag="KKO08_003495"
     CDS             complement(8974..>9951)
                     /locus_tag="KKO08_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBD6319603.1"
                     /translation="IACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2349 a         2677 c         2506 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa t
//
LOCUS       DAECNO010000126         9928 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.126, whole genome shotgun sequence.
ACCESSION   DAECNO010000126 DAECNO010000000
VERSION     DAECNO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.126"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..339
                     /locus_tag="KKO08_003496"
     CDS             <1..339
                     /locus_tag="KKO08_003496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319604.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            527..1642
                     /locus_tag="KKO08_003497"
     CDS             527..1642
                     /locus_tag="KKO08_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319605.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2321..2539
                     /locus_tag="KKO08_003498"
     CDS             2321..2539
                     /locus_tag="KKO08_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBD6319606.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2757..2999
                     /locus_tag="KKO08_003499"
     CDS             2757..2999
                     /locus_tag="KKO08_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319607.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="KKO08_003500"
     CDS             complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="KKO08_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBD6319608.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="KKO08_003501"
     CDS             complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="KKO08_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBD6319609.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="KKO08_003502"
     CDS             complement(4887..5789)
                     /gene="fdoH"
                     /locus_tag="KKO08_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBD6319610.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="KKO08_003503"
     CDS             complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="KKO08_003503"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8265..8267),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD6319611.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9046..9879
                     /gene="fdhD"
                     /locus_tag="KKO08_003504"
     CDS             9046..9879
                     /gene="fdhD"
                     /locus_tag="KKO08_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBD6319612.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2395 c         2543 g         2500 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca
      901 taccgcaatg atgagggagg catgtaagga attaccgcag tcagtggaat atgttccttt
      961 agcctgtgaa gcgcatagcc atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat
     1021 catggcgaca ggtgtgggct tggcattaat gtatgcaaca tcatcaggac cgtattatgc
     1081 gcaaagtctt tctggttcgc cggatatcga gatgataaaa aatcgcatgg ctgaattgtt
     1141 taaccagtcg gatatttctg tgcgcaacaa atatagcccg gcttttgata aatggaatga
     1201 actggtagat aaattaaatc acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat
     1261 tttagcgacg gcgataaatg aaactcttga gggggatacg aatgttgccg ttgtgatggg
     1321 gtgtaaatct gcgaaagatc gcacgatatc cattgtcctt ggcaactcga tgttgcaaac
     1381 actccttgaa aaacgcctcg ccgatagtgg agaaatagaa aatctttttg atcaacaggg
     1441 atattttagt tgcgattcat taacacccga agaattaatg atgttaaaag acctttttga
     1501 tattcgagtt cttcatgtct ctaacaaatt caatgttgga ttgcaaggta atatcaacac
     1561 tgatgtttta caagattcgt tttttaaaaa tgtcgatttc attcatgaat ataactcgta
     1621 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
     1681 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
     1741 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
     1801 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
     1861 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
     1921 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
     1981 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
     2041 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgcaaata
     2101 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
     2161 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
     2221 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
     2281 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
     2341 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
     2401 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
     2461 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
     2521 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
     2581 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     2641 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     2701 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     2761 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     2821 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     2881 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     2941 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     3001 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     3061 tgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     3121 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     3181 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     3241 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     3301 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     3361 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     3421 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     3481 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     3541 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     3601 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     3661 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     3721 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     3781 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     3841 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     3901 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     3961 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     4021 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     4081 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     4141 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     4201 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     4261 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     4321 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     4381 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     4441 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     4501 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     4561 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     4621 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     4681 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     4741 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     4801 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     4861 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     4921 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     4981 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     5041 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     5101 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     5161 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     5221 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     5281 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     5341 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     5401 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     5461 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     5521 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     5581 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     5641 gaacaagcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     5701 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     5761 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     5821 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     5881 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     5941 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     6001 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     6061 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     6121 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     6181 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     6241 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     6301 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     6361 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     6421 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     6481 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     6541 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     6601 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     6661 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     6721 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     6781 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     6841 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     6901 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     6961 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     7021 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     7081 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     7141 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     7201 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     7261 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     7321 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     7381 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     7441 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     7501 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     7561 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     7621 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     7681 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     7741 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     7801 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     7861 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     7921 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     7981 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     8041 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     8101 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     8161 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     8221 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     8281 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     8341 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     8401 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     8461 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     8521 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     8581 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     8641 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     8701 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     8761 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     8821 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     8881 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     8941 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     9001 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     9061 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     9121 cgtgatgatt tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg
     9181 gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     9241 gcgctcggtt tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg
     9301 gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt
     9361 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc
     9421 gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt
     9481 gatctcaaca aactggatga tgcattacgt catctcaacg attttcagcc agtggggcga
     9541 ctgactggtt gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg
     9601 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     9661 ggggaaagct ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg
     9721 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt
     9781 gctgtagaag tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg
     9841 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     9901 atattaaatt gcaggactta ttcatttc
//
LOCUS       DAECNO010000127         9910 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.127, whole genome shotgun sequence.
ACCESSION   DAECNO010000127 DAECNO010000000
VERSION     DAECNO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.127"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(165..905)
                     /gene="pflA"
                     /locus_tag="KKO08_003505"
     CDS             complement(165..905)
                     /gene="pflA"
                     /locus_tag="KKO08_003505"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBD6319613.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1097..3379)
                     /gene="pflB"
                     /locus_tag="KKO08_003506"
     CDS             complement(1097..3379)
                     /gene="pflB"
                     /locus_tag="KKO08_003506"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBD6319614.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3434..4291)
                     /gene="focA"
                     /locus_tag="KKO08_003507"
     CDS             complement(3434..4291)
                     /gene="focA"
                     /locus_tag="KKO08_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBD6319615.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4697..6457)
                     /locus_tag="KKO08_003508"
     CDS             complement(4697..6457)
                     /locus_tag="KKO08_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBD6319616.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6587..7213
                     /locus_tag="KKO08_003509"
                     /pseudo
     CDS             6587..7213
                     /locus_tag="KKO08_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7412..8500
                     /gene="serC"
                     /locus_tag="KKO08_003510"
     CDS             7412..8500
                     /gene="serC"
                     /locus_tag="KKO08_003510"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBD6319617.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8571..9854
                     /gene="aroA"
                     /locus_tag="KKO08_003511"
     CDS             8571..9854
                     /gene="aroA"
                     /locus_tag="KKO08_003511"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBD6319618.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2369 c         2484 g         2536 t
ORIGIN      
        1 cggtattcga tggggaagaa cttcacgcga gctattatca gtcgttatta tctcaggtac
       61 gggatatggt agatgcacta taagatgtgt taaaaacgct gtagcagaat gaagcgcgga
      121 ataaaaaagc ggcaactcaa taaagttgcc gctttacgag gaaattagaa cattacctta
      181 tgaccgtact gctcaagaat gcctttcacg cgttccatgg tctctttctt cggtggttta
      241 acaccatcga gtttgtattc ttcacccatc gccacccatt tgtgtttgcc cagttcgtgg
      301 taggggagaa gttcgatttt ctcaacgtta cccatatcac gggtaaattc accgaggcga
      361 tgtgccgaat cgtcatcgtc agaccagcct gggacaacaa cgtagcggat ccacaccttc
      421 acatttttgt tcgccagata tttagcgaac tccagcgtgc ggtggttgga aactccaacc
      481 agattttggt ggatctcgtc gttcatctgt ttgagatcga gcattaccag gtcggttact
      541 tccagcagtt catcaatcac cggatcgtaa cgacgaacaa aaccgttggt atccagacag
      601 gtatgaatgc cttctttttt acaggcgcgg aaccagtcac gaacaaactc agcttgcagg
      661 attgcttcac cgccggatgc ggtaacgccg ccgccggaag cgttcataaa gtggcgatag
      721 gtcaccactt ccttcatcaa atcttcaacg gtaacttctt taccgccatg cgtgtcccag
      781 gtgtcgcggt tatgacaata caggcagcgc atcaggcagc cctggaaaaa ggtgataaag
      841 cgaatacctg ggccgtctac ggttccacag gattcaaagg agtgaatgcg accaataact
      901 gacattgcgg tgtttctcca gatgtggccc atctgaggcc gtgttggtgc gcagctcgaa
      961 ggctacgtcg agtctgtttt ggcagtcacc ttaaagtata gatagctgac aaaaaaggct
     1021 ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt gtacgctttt tactgtacga
     1081 tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg tcctgctgct
     1141 gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg gtcagctgcg
     1201 gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc acgttaacgt
     1261 tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc atcagaccag
     1321 ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg atagagaagg
     1381 tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa gtcagagacg
     1441 ctacagcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg aacggcgcgc
     1501 cagcacgacg accgtctggg gtgttaccag ttttcttacc atacacaacg ttagaagtga
     1561 tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc tgaattttct
     1621 tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatca ttgttaccaa
     1681 actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg tcacgaatcg
     1741 gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac agaccagcga
     1801 taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga gaggcttcgt
     1861 agctgtactt gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac tgtttagcca
     1921 gccagtccat gaagtgatcc atgcgctcca tcacttcatc gtagctcagg agatcgcctt
     1981 tgatcggttc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg ccgccgttga
     2041 ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc atttgtttac
     2101 caacgatcat cgggcttacg cagcaagcga tagcgtagtc atcgttgttg aagtccggac
     2161 gcatcaggtc atcgttctca tactgcagag aagaggtgtc gatggacact ttagcggcga
     2221 atttcttgaa gttcagcggc agtttttcag accacagaat ggtcatgttc ggttccggag
     2281 acggccccat agtgtacagg gtgttcagga aacggaagct gtttttggta accagggtac
     2341 gaccatcgag gcccatacca ccgatagatt cggttgccca gattgggtcg ccagagaaca
     2401 gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg accaggtggt
     2461 caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca cgttcgatgt
     2521 acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc tgagacttaa
     2581 cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg gtagccggac
     2641 cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga gcgcggtgct
     2701 gttcagcgat ttcttcgcgc agacggatag tctgttccag gtttacgccg ttttccagat
     2761 cagcctgtag agaggtgaac tgagcgtatt tgtctttcat caggtagtcg ataccgtaca
     2821 gcgcaacgcg acggtagtca ccgatgatac ggccacggcc ataagcatct ggcagaccgg
     2881 tcagaacacc ggatttacgg caacgcagga tgtccggagt gtaaacgtcg aacacgccct
     2941 ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcgggtcc agttcgcggt
     3001 tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata agagcacgtt
     3061 tcagcggagc ttcagtctgc agaccaacaa ctttttccaa cgctttattg atgtagccag
     3121 cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa atcaactggc gcgtgagtgc
     3181 ggttttccag tttaacacct tccattactt tgtcccacag ggtggtggtc gcttcagtag
     3241 cgccagccag gaaggactcg tcaccctcgt acggcgtgta gtttttctga atgaagtcac
     3301 ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct gtggctaact
     3361 tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta tttactgcgt
     3421 acttcgacaa ccattaatgg tgatcgtttt cacgcaggta aatgacccag tatgtcaacc
     3481 caaccaacaa accaccgccg ataatattac cgatcgtaac cgggatcagg ttatcagtga
     3541 tgaagttcat cacggtcagg tgagaaaaat tttccggtgc agaaccgaca gcggtccaga
     3601 attccggaga tgcgaagtcg cggattacaa tacccatcgg gatcataaac atgtttgcga
     3661 tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata atgaacgctt
     3721 tgtccatcag gctgcggcca gaataactca tccatactgc cagacatacc atcaggtttg
     3781 ccaggatacc aagacagacg gcctcaataa aagtatggtg cactttgtgg tcggcggttt
     3841 gtaggacgtt tagtccccat tgaccatttg cggtcatata ctcgccggaa agccacatta
     3901 aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc cagtttttag
     3961 ccaactgaca ccaggtgatg cgcccactcg ccttagcaac aacaatcaac actgtggaag
     4021 taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag caaatgccgc
     4081 caaccagttt tgccatgccg aagggcattg tgcctgtgcc agtggttgct gtgatataga
     4141 agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa gtcttaagcg
     4201 gatgtttcgt tgctttatag acacccgcct cttcggccac tttggccatt gcagcaggaa
     4261 gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta agtcggcgac
     4321 gagatactaa caaagcatta tagatgagaa attgatatag atcatatctc gcctggctta
     4381 taggcccgta actcgcatgg tttttatgca aatacggagt aactatttga ttatccaaat
     4441 aaaaataaat tttaaaaatt aacaaatgag ttgaattttt tccgcatcct ccgctaaaac
     4501 agttaattaa aagggagcat caggcgaata aagtaacaat atcgatcgta tttattaaat
     4561 acaaattacc gatatttaac cttataatta caattatttt attaatgcaa atatatgtaa
     4621 agcggggcat taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg
     4681 cgtaggctat ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt
     4741 aggccttcaa caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca
     4801 ggccgtaaaa cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc
     4861 cgtacatgcg aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca
     4921 gtaataacaa cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg
     4981 agttaaactc catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca
     5041 gcgccagcat ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg
     5101 tcgccagacc caacagctca cgcacgcggg taaagtcatc aaaatcttct tcatccagtt
     5161 gctcgatgag gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg
     5221 tttcacgtaa atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa
     5281 tatcggacat gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg
     5341 caatataaac ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt
     5401 ccgtggtgcc ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt
     5461 cccaggagat taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga
     5521 cttcttcatc atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt
     5581 gcagcagctc ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag
     5641 aggcaaagca ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc
     5701 cgccaaggga accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg
     5761 cttcaaccac cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc
     5821 tttcagcaat aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc
     5881 gtgcttcgtt gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga
     5941 tattcatcgg aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa
     6001 taccacgatc ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct
     6061 ccggatcgta aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt
     6121 cgctcagtgg gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt
     6181 cgcccagcca gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga
     6241 gtgcagaagc cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc
     6301 actctttgtc gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct
     6361 cttcaatctg aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca
     6421 gagcggcatc tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga
     6481 aagcgcgcaa taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa
     6541 ctgttgacta tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga
     6601 tctccaccgt atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg
     6661 tagtctttat acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg
     6721 cgacagatgt cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat
     6781 gtggcgtttt atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg
     6841 ttgttatacc gcctggtaat atgatggcga gctggcctgg tcgaaactca ataactccaa
     6901 catgacggaa tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca
     6961 ggtacgtctg gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga
     7021 caaggtgaaa ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt
     7081 gccggagtcg gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg
     7141 ggaaaaacaa ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa
     7201 acgggtgacc tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt
     7261 gtgatgcaag ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa
     7321 tgataaaacc gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg
     7381 ggttaatagc aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg
     7441 tccggcaatg ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa
     7501 cggtcttggt acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc
     7561 agaggaagcc gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt
     7621 attctgccat ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga
     7681 taaaaccacc gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc
     7741 gaaaaaatac tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc
     7801 ggttaagcca atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc
     7861 gaatgaaacc atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt
     7921 ggtcgccgcc gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg
     7981 tgtaatttac gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt
     8041 tcgtgaagat ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat
     8101 cctcaacgat aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg
     8161 tctggtcttt aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca
     8221 gcaaaaagca gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt
     8281 ggcgaaagct aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga
     8341 caaattgttc cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt
     8401 ggtcggcgga atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct
     8461 gacagacttc atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc
     8521 cccacagcca gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc
     8581 tgacgttaca acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg
     8641 tttctaaccg cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc
     8701 tgctggatag cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct
     8761 atacgctttc agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg
     8821 cagaaggtgc cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg
     8881 cagctctttg tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc
     8941 gcccgattgg tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg
     9001 aacaagaaaa ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg
     9061 ttgatggctc cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc
     9121 cggaagatac ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca
     9181 cactcaatct gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg
     9241 tcgtaaaagg cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat
     9301 cttcggcttc ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg
     9361 gtattggacg taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg
     9421 gcgcgaccat ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta
     9481 ttgatatgga tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat
     9541 ttgcaaaagg caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc
     9601 gcctgtttgc gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg
     9661 attacattcg tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg
     9721 atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc
     9781 ttgatcccaa atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta
     9841 gccaggcagc ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt
     9901 cggcacattg
//
LOCUS       DAECNO010000128         9850 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.128, whole genome shotgun sequence.
ACCESSION   DAECNO010000128 DAECNO010000000
VERSION     DAECNO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.128"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="KKO08_003512"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="KKO08_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBD6319619.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="KKO08_003513"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="KKO08_003513"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBD6319620.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="KKO08_003514"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="KKO08_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBD6319621.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2405..2608)
                     /locus_tag="KKO08_003515"
     CDS             complement(2405..2608)
                     /locus_tag="KKO08_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319622.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2686..3780
                     /gene="ddlA"
                     /locus_tag="KKO08_003516"
     CDS             2686..3780
                     /gene="ddlA"
                     /locus_tag="KKO08_003516"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBD6319623.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3804..4016)
                     /locus_tag="KKO08_003517"
     CDS             complement(3804..4016)
                     /locus_tag="KKO08_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBD6319624.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4276..4584
                     /locus_tag="KKO08_003518"
     CDS             4276..4584
                     /locus_tag="KKO08_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBD6319625.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="KKO08_003519"
     CDS             complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="KKO08_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBD6319626.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="KKO08_003520"
     CDS             complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="KKO08_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBD6319627.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7322..8479
                     /gene="ampH"
                     /locus_tag="KKO08_003521"
     CDS             7322..8479
                     /gene="ampH"
                     /locus_tag="KKO08_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBD6319628.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8480..8983)
                     /locus_tag="KKO08_003522"
     CDS             complement(8480..8983)
                     /locus_tag="KKO08_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6319629.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9071..9598)
                     /locus_tag="KKO08_003523"
                     /pseudo
     CDS             complement(9071..9598)
                     /locus_tag="KKO08_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9618..>9850)
                     /locus_tag="KKO08_003524"
     CDS             complement(9618..>9850)
                     /locus_tag="KKO08_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6319630.1"
                     /translation="MSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYI
                     EMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         2446 a         2512 c         2329 g         2563 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac accggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat
     2281 agtgtctgga agtattattt ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt
     2341 gattaccagc gaaacgtttc tcttatttaa ataattaccc acacaaaata taggctttta
     2401 attatcaacc tttattataa ataaaagcta tgaaatatat tctcttcgtt aacttataat
     2461 taagagaaaa aaactcaccg cattgccatt taagccagga ataaattagc ttcccgttca
     2521 tcatttttgt gatcttgcgc acatctttga gataacgcac tttcttcacc agtacgttgc
     2581 acgctttgtc agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt
     2641 cgtggtaatg tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg
     2701 gtaggaatcg tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa
     2761 aacattgtcg atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa
     2821 caagggcaat ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc
     2881 catattgcgt tgcgcccttc ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat
     2941 cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt
     3001 gtccacggta cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta
     3061 ccgtttgtag gttctgatgt tctggcttca gcagcctgta tggataaaga tgtcaccaaa
     3121 cgtctgctac gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat
     3181 cgtcacaaca tcagttttgc cgaagtggag tctaaactgg ggttaccact gtttgtaaaa
     3241 ccggctaatc agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca
     3301 attgccgtcg atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa
     3361 ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc
     3421 gagatcgtac tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc
     3481 gcgaaagtgg tagttccggc agccattgca ccagagatca acgataagat ccgggcgatt
     3541 gccgttcagg cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta
     3601 accccagaga acgaagtggt gatcaacgag atcaacacac tgcctggctt caccaacatc
     3661 agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt acaccgatct gatcacacgt
     3721 ttgattgagc tggcgctgga gcgtcacgct gcggataacg cattgaaaac cacaatgtaa
     3781 tattttaacg cccgataata ttcttattgg gcgttttcag tttcttcatc gcgacgacga
     3841 ataatcaacc ccgccagcca gaaactaatc acccacgtca ccagaccgac ggcataggtc
     3901 tgccaacctt ttgcttcaaa tcccagtacc cccaccacgc cattaagaat gaatataagg
     3961 ccaatggcga acgcatagta gtgccagtca cggcggattt ttacaggcag attcatggca
     4021 gctccgttaa cgaaaaacaa catccttgca tattctgtaa caaccgtctg tggtgtagac
     4081 aacattttcg taattattaa taaatttaac ttaaggttgc cattttgtga ctcacaacga
     4141 agattcacgc ttcgggaata ttcccaacat caatttacca tgattctgat attgacaatc
     4201 taaatgacct gtcagacttg ccataagccg ctggtatcac accagaagag aagcagatga
     4261 cttccggagg tttttatggc tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat
     4321 cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt
     4381 tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta
     4441 atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt
     4501 ttcgggctga tcccgttttt agcaggctgc ctcatttttg cagtggtggc gctatggctg
     4561 cactggcgac atcgccgcca gtaaaattta cgttgccaga tgcggcctga aacgccttat
     4621 ctggcctgac attcctgttt gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc
     4681 gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt cgggctttcc agttgaatgc
     4741 caggcaacat ttcgcggggt agtgatttac ccgttttcgc ttcggcaagt tgataaactt
     4801 tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc tttctctaac tgacggcgaa
     4861 tttcgctgtc gctcatcccc agttttcccg ccagtttgcg cgttgccagt tcggttttcc
     4921 tgggttcttt actgtcgtaa cgaattaaat caccgtccag tgccagcttc acgccgctgg
     4981 ccttactgac tgcgttctga aacgcggcat tacggctggc gtaccagcca gcgttaaaat
     5041 cagcaaagcg gtatatcggt gcgctatagc tggcgggata attcagtaaa tggtaagtac
     5101 caaaccacaa cccgccgcgg cgactgaaga cttcctgacg aactgtaccg tccattttcc
     5161 acggataccc tttggtatgc tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg
     5221 tgcgcaccgg attaagcgaa ccaaacagcg tctgccccat tggtaccatg ctgatcagat
     5281 cgtcaaaaat cgcgctcaat tgcttttctg tacgcaccga atctaaccgt tcgctataac
     5341 ttttgccgtt tggcgatttg atttttagcg cggtatgaac cagaaaagca ggaatgtgca
     5401 tccgttcagc acgacggtca atctcttgcc aggcgatctt gcttaagccc ggaacggccg
     5461 gatctgcctg ataatttgac tcctgctgcg ccaccgccag caccgagtag acgttttcca
     5521 gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag atctttcgcc caggcatcgc
     5581 ggtctttcac gctcgcaggc atcttctgcc gcacgacgct cgctacatct atcgcttttt
     5641 cgcccttttt caacggttgc ggagcctgac tactacaccc cgctaaaacc agaaccgcca
     5701 acaatgacag cgaagataga tgattgacgc gcgacattac ttctcctttt tagctcaagg
     5761 tatgggttac ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt
     5821 tgtagataga catcaactca accagtgtgg tccatgagtt aatcaggtac tggaaagcac
     5881 cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg
     5941 caacaattga cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa
     6001 tgcgggcgat gttgaaatac atatagtgaa aatagaggcg aaaatagttt ttccgtacgg
     6061 cgctaaacag ctcgcgtacc gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa
     6121 ccagctcttt acggtaggcg gcctctactc gctggttttt aaactccagc cccggcagtt
     6181 tgatccctac cactgccagc aatccggtcc ccatcagcga ccagacgata gcggcaatca
     6241 ccagaccata cggaatgtgc ccgataatcg gcagctccgg cacatgcgcg gagagcgtta
     6301 ccagcaccgg caggaaggcg atcaacgtca ttatggcgtt gataaaactg acgcccatat
     6361 tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga
     6421 tatgacgcag ttgttgccag ttcgccatgt aatattcgtt catcgccgta cgccagcgga
     6481 acacgtagtg actgacaaag aagttgttca gtacactgat caccactgcg atcagcgcaa
     6541 tccccagaaa gacgcccact tcgcggtaaa attgttcgat agtgacttta tgcggcgaac
     6601 ttagcgcggt ttgaatcaga tcatagaacg gcgcatacca ggcgttgacg gcgaccccga
     6661 cttccaccaa aaaccaagtg acgaagataa tcagcgcagt accgagtatt gaccagtatt
     6721 gccaacggtg cgggctgtag ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt
     6781 agtaagcgta aaaaatcagg aaatccaacg accagaaacg cgcggcgcta atcgggatct
     6841 gcccggaagc gccggtgata tgtgccaccc agtcaccccc accggcttgc cagaagataa
     6901 cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt tcccggcttt gggaaaaaag
     6961 acttaaacat atgcactcct gctaacttct tatcgtttta acaactgctg tgtattaacc
     7021 gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg
     7081 cttagttcgc cagcctgacc ataagaattg tttcgacgga tttcacaaac caaagattgt
     7141 ttacataagc ggattaacac gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa
     7201 gatcgtgttt acgcgccgtt acgtttttaa cactattatt gcactaattc gccctttgca
     7261 atccatcaat gagtagtata aatacgctca gttaccttca ttcaatctat ggacaccacc
     7321 gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca
     7381 tgcggcgcag ccgatcactg aaccggagtt tgcctctgat attgtcgatc gttatgccga
     7441 tcatattttt tacggcagcg gtgccacggg gatggcgctg gttgttatcg acggtaacca
     7501 gcgcgtcttt cgtagttatg gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga
     7561 ttcggtcgtg cgtattgctt ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt
     7621 gctcgaccag gggaccgtaa agttaaacga tccgctaagt aaatatgctc cgccaggcgc
     7681 acgcgtgccg acctacaatg ggacgcccat taccctagtg aatctggcaa cccataccag
     7741 cgccctgccc cgtgaacagc ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac
     7801 gcgcgagcaa cgctggaaat acctttctac ggcgaagctg aaagccgcgc caggcagtca
     7861 ggcggcgtac tctaaccttg cgttcgacct gctggcagat gcactggcga atgcgtccgg
     7921 caagccttat acccagttgt ttgaagagca gattacccat ccattgggga tgaaagacac
     7981 cacctacacc ccctcaccgg atcagtgccg ccgtttaatg gttgctgagc gcggtgccag
     8041 tccgtgcaat aacacgctgg cggcaattgg cagcggcggc gtctattcca cgcctggcga
     8101 tatgatgcgc tggatgcagc agtatttgtc atctgatttt tatcagcgca gtaaccaggc
     8161 agatcgcatg cagacgttga tttatcagcg cgcgcagttt acgaaagtga ttggcatgga
     8221 tgtccccggc aaagcggatg cgcttggttt aggctgggta tatatggccc cgaaagaggg
     8281 tcgtccgagg attattcaga agacaggcgg tggtggcggt ttcattacct atatggcgat
     8341 gatcccgcag aagaatattg gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt
     8401 taaaaatatg agcgatggca tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt
     8461 ggttatcccc gcatcctgat taatactctg aaggcaaacg gttgatagcg accagtttgc
     8521 ctttattcat ttcgatatag ccgcctttac gcaaagcagc gagaacttct gcgacgacag
     8581 aacgcgatat gcgtgtacgt tgatggatat aattcatcac accaatacgt gatcgcaatt
     8641 cttcattcca gtcaatcatt gataataatg ttgcgcgggt ttgtttggct ggtagatgat
     8701 atcccgtaca atttccttct gatattaatt tgtatggtat atcgtttttc attaaaccat
     8761 cagccagccc cataatataa ggagcctggg taataccgat aagtacgttt tcttctctac
     8821 gcagagagat aactccttct aaaataacga aagtatcttc gttattgaca tcactggaaa
     8881 atataacttg cttttcatta ttaaactcga agcgcgtacc gtatctggac aaacatttat
     8941 cgagcttacc aaattcctga agaggtttaa ctacagataa catttgcgcg tcctttgcag
     9001 taatgcccgt caaatccttg acgggcatta tttagattaa attaccagta tttcttcgga
     9061 gtgaagaata ttaccaagta tatttaacac ccacgttcgc ggaccagtct tgatctacgt
     9121 caccaccacc gaggtagtta gcatcggtat aggcgctgaa gttcttggtg aagctgaact
     9181 gagtacccag accaacacgt accgcagacc cttcagtacc gttatcgatg gaatcaccgt
     9241 tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc cagtttgaag tacggagtca
     9301 gagcctgatc ttcgctgtag gtgaaggtat aacctgcatc tacacccagt tcataacgca
     9361 tgctgtcgta aggctgaccg tcaactttca tgtcgttgct cagctggtag tcatcaccag
     9421 actggaacag accagaaatg ctgccgtaag gagtcacgta accagcatca cccagtttga
     9481 agtcgtaacc ggttttcaaa ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt
     9541 aagtaccgtt gctcatggtt gcagacaggt cgttgttgaa gtgagagtag ctcaagctat
     9601 gaatcgccac ggataatcta gacacttccg agccgttgat aatactggtt ttcatattct
     9661 gtcggtgaca tctgttcgct agaaccatgc cgacgcttac tgttataaaa catttcgatg
     9721 taatcaaaaa tatcactgcg ggcttcttcc cgcgttccgt agatcttttt ctttatccgt
     9781 tcacgtttca acaactggaa aaaactttct gcaaccgcat tatcatggca gttaccgcga
     9841 cggctcatgc
//
LOCUS       DAECNO010000129         9644 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.129, whole genome shotgun sequence.
ACCESSION   DAECNO010000129 DAECNO010000000
VERSION     DAECNO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.129"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KKO08_003525"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="KKO08_003525"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBD6319631.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="KKO08_003526"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="KKO08_003526"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBD6319632.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /gene="frdB"
                     /locus_tag="KKO08_003527"
     CDS             3487..4221
                     /gene="frdB"
                     /locus_tag="KKO08_003527"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD6319633.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="KKO08_003528"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="KKO08_003528"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD6319634.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="KKO08_003529"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="KKO08_003529"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD6319635.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /locus_tag="KKO08_003530"
     CDS             5060..6193
                     /locus_tag="KKO08_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD6319636.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /gene="blc"
                     /locus_tag="KKO08_003531"
     CDS             6282..6815
                     /gene="blc"
                     /locus_tag="KKO08_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBD6319637.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KKO08_003532"
     CDS             complement(6812..7129)
                     /gene="sugE"
                     /locus_tag="KKO08_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBD6319638.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KKO08_003533"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="KKO08_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBD6319639.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KKO08_003534"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="KKO08_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBD6319640.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KKO08_003535"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="KKO08_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBD6319641.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="KKO08_003536"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="KKO08_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBD6319642.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2473 c         2459 g         2261 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tctt
//
LOCUS       DAECNO010000130         9625 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.130, whole genome shotgun sequence.
ACCESSION   DAECNO010000130 DAECNO010000000
VERSION     DAECNO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.130"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KKO08_003537"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="KKO08_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBD6319643.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KKO08_003538"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="KKO08_003538"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBD6319644.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="KKO08_003539"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="KKO08_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KKO08_003540"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="KKO08_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBD6319645.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KKO08_003541"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="KKO08_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBD6319646.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="KKO08_003542"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="KKO08_003542"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBD6319647.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="KKO08_003543"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="KKO08_003543"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBD6319648.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="KKO08_003544"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="KKO08_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="KKO08_003545"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="KKO08_003545"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBD6319649.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2269 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaat
//
LOCUS       DAECNO010000131         9494 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.131, whole genome shotgun sequence.
ACCESSION   DAECNO010000131 DAECNO010000000
VERSION     DAECNO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.131"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..190)
                     /locus_tag="KKO08_003546"
     CDS             complement(<1..190)
                     /locus_tag="KKO08_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBD6319650.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="KKO08_003547"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="KKO08_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBD6319651.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="KKO08_003548"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="KKO08_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBD6319652.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="KKO08_003549"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="KKO08_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBD6319653.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="KKO08_003550"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="KKO08_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="KKO08_003551"
     CDS             complement(3262..3381)
                     /locus_tag="KKO08_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBD6319654.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="KKO08_003552"
     CDS             complement(3342..3527)
                     /locus_tag="KKO08_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319655.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="KKO08_003553"
     CDS             3628..3801
                     /locus_tag="KKO08_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319656.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="KKO08_003554"
     CDS             3803..4147
                     /locus_tag="KKO08_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBD6319657.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="KKO08_003555"
     CDS             4151..4486
                     /locus_tag="KKO08_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBD6319658.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="KKO08_003556"
     CDS             complement(4543..6864)
                     /locus_tag="KKO08_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBD6319659.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="KKO08_003557"
     CDS             complement(6981..7190)
                     /locus_tag="KKO08_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319660.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7941..9464)
                     /locus_tag="KKO08_003558"
     CDS             complement(7941..9464)
                     /locus_tag="KKO08_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBD6319661.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2660 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa taca
//
LOCUS       DAECNO010000132         9293 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.132, whole genome shotgun sequence.
ACCESSION   DAECNO010000132 DAECNO010000000
VERSION     DAECNO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.132"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="KKO08_003559"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="KKO08_003559"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBD6319662.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQSFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..3047
                     /locus_tag="KKO08_003560"
     CDS             1647..3047
                     /locus_tag="KKO08_003560"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBD6319663.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            3417..4241
                     /gene="gadX"
                     /locus_tag="KKO08_003561"
     CDS             3417..4241
                     /gene="gadX"
                     /locus_tag="KKO08_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBD6319664.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            4609..5337
                     /gene="gadW"
                     /locus_tag="KKO08_003562"
     CDS             4609..5337
                     /gene="gadW"
                     /locus_tag="KKO08_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBD6319665.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="KKO08_003563"
                     /pseudo
     CDS             complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="KKO08_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(8838..9095)
                     /locus_tag="KKO08_003564"
                     /pseudo
     CDS             complement(8838..9095)
                     /locus_tag="KKO08_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         2430 a         2161 c         2278 g         2424 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaagttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg
     2761 acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     2821 tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     2881 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     2941 actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc agcgatcacc
     3001 cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa cataacgttg
     3061 taaaaaccga atgcccagcc tttaaaaaaa cagctgggca ttcggttgct tattaatcgc
     3121 aataatatat tggctgtcta ttcatcgtgt tgataagata tatgttatat gaatgtttat
     3181 taaatgtagc gattatcttt aatgtcacga aaagggatta tttgcttgct attaatttca
     3241 ctgcgttctt aaacacgttt ttgtattata aatccagtca tcctgcccgt agaacatcac
     3301 acattatcat cctgttctcc cgcttcgttt aaatttattt atcaatcaat ttgacttaag
     3361 agggcggcgt gctacattaa ttaacagtaa tatgtttatg taatattaag tcaactatgc
     3421 aatcattaca tgggaattgt ctaattgcgt acgcaagaca taaatatatt ctcaccatgg
     3481 ttaatggtga atatcgctat tttaatggcg gtgacctggt ttttgcggat gcaagccaaa
     3541 ttcgagtaga taagtgtgtt gaaaattttg tattagtatc aagggatacg ctttcattat
     3601 ttctgccgat gctcaaggag gaggcattaa atcttcatgc acataaaaaa atttcttcat
     3661 tactcgttca tcactgtagc agagatattc ctgtttttca ggaagttgcg caactatcgc
     3721 agaataagaa tcttcgctat gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt
     3781 tatctgtttt tcttgaggat gagcacttta taccgctgct tttgaacgtt ttgcaaccga
     3841 acatgcgaac acgagtttgt acggttatca ataataatat cgcccatgag tggacactag
     3901 cccgaatcgc cagcgagctg ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag
     3961 aagagacatc atattcacag ttgcttactg agtgtagaat gcaacgtgct ttgcaactta
     4021 ttgttataca tggcttttca attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt
     4081 cgtatttcat ttacgtcttt cgaaattatt atgggatgac gcccacagag tatcaggagc
     4141 gatcggcgca gggattgccg aaccgtgact cggcggcaag tattgttgcg caagggaatt
     4201 tttacggcac taaccgttct gcggaaggaa taagattata gagttttact cagacataaa
     4261 aaaaacccgg cataggggac cgggaagagg atagtctgcc gtctccagac taataaaccg
     4321 ttataacact ccctgttggc acgggaaact ttgtgctctc agtaagttaa atataacttt
     4381 tactggaaat aagatcagcc atttttttat aaacataagc tatacgctgt gcgaaaatat
     4441 attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt tgtggataaa
     4501 ttttggtcct accaaatctg gcagtttttg cgctaagaaa cagtctgtca tcatttcatt
     4561 agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat gactcatgtc
     4621 tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca aaaaatatcg
     4681 ctgcataacg agagtatcct gctgctggag aaaaatttgg cagacgattt tgcgttttgt
     4741 tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta cttacaaaat
     4801 attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctgtt aattaaccag
     4861 tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc cggggtaaat
     4921 tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc tcataagaaa
     4981 gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt tgaacgcctt
     5041 attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag aatgtatacc
     5101 agcgagagtc tcatcaaaaa aaagttgcag gatgaaaata cctgtttcag taaaatatta
     5161 ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat tcctctgcat
     5221 actattgcgg aaaaatgtgg ctatagcagt acgtcgtact ttataaacac atttcgacaa
     5281 tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt ttcctgacat
     5341 attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac tcttccctga
     5401 tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg tataggtatt
     5461 tttaggagga aaagactcgg catgtttggg attattaagc tgacaattca tacgattacg
     5521 ggaatgtggg tcagtattgt gttgttcaaa ttgatgacga atggctggag tggtttctac
     5581 tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt gagtggtgaa
     5641 tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc atttacacgt
     5701 tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac cggaacgaag
     5761 taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc taccgcgttt
     5821 tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag aataaaggcc
     5881 agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc
     5941 ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag gatggcgttc
     6001 ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc attacttaag
     6061 ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac caacatcacc
     6121 gagaacggaa ttgaccagct ctcatagagt gcggcgaggg cgaggaacac cacgaccagt
     6181 gaaatggcat acagtgcagg agcctggttt gaggataacg cttcctgata cgacagtccg
     6241 gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat aaatttcata
     6301 gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga cgggatgccg
     6361 ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg tagacgagta ggcagaaagc
     6421 ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc cggcaacata
     6481 cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt gaggaagtcg
     6541 ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga cagcgccaca
     6601 cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt atcttccagg
     6661 ccgttcgggc gtaccccggt gacctggtca ggcgattgcg ctgccagtga taacagctcg
     6721 tttcgtgcct aggttagttt ttcgtgcccc aggttaccgt tgtccagcag ttccatatca
     6781 aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa gacgacggct
     6841 ttattgatac tgcttaacgc aatcattgcc cgctgaatga tcgcggtaac cgagttttcc
     6901 tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt gttttgcccc
     6961 tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc tttctctttt
     7021 gtaagataat aatccgtcac ctgttgcagt actttcgtgg tgttaaccat ggtggcaccg
     7081 gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa gaaagaggtc
     7141 ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagac caccatgtag
     7201 cgaccggtac aacgcaacag cgagcgggtg ctatcggtat agtgctgagt tgatttttca
     7261 aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc cggcgtggct
     7321 ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac tgaaagccgc
     7381 atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc accgctcata
     7441 aaggccatcg gcataaacac tgcagaaaga acaacggcaa tcccgaccag cgcacgttgg
     7501 atctgcccca tcgatttatg cgtcgcttcc ttcggcggta gcttatcttc cgcaatgaca
     7561 cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat cgccagcacc
     7621 atcccgaaca tagtcaacgt gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc
     7681 ccgagaataa ccaccggtac ggcaattgtc gggatgatgg tggcacggaa attctgcaaa
     7741 aacagataaa tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt gaaaacttcc
     7801 tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg
     7861 gggaaatagg ctgataagcg gttcagctcc tctttgactg cccgcgaggt atccagggcg
     7921 tttgctccgg cagccagttt gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc
     7981 acggtggaat aatcttccgc cccaagttcg acgcgggcga catcacgcag cagcacttgc
     8041 gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt ttgcagacgc
     8101 gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat gccacccagt
     8161 tgaccaccag aaatctggtt gttttgcacc ttaatctggg aaataacatc ggaaggtacc
     8221 aggttatatt tattgagttt ttgcgggtcc agccagatac gcatggcgta ctcggaacca
     8281 aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat attagacgct
     8341 acatagtccg cgatatcgta ctggttgagg ctgccgttat cagaaataaa cgccgctacc
     8401 atcaggatat tactgctcga cttatcgacg ctaatcccct gttgctgcac cgcttcaggt
     8461 aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat atcaggagat
     8521 gtcccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact ggttgaagac
     8581 atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt gaccgagtct
     8641 tctaccgttt gcgcatcggc acccggatag gtagcgctga cggtaatggt cggtggcgca
     8701 atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc aagcatcata
     8761 ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc catgaaagtc
     8821 cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa
     8881 attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat cacccgatcg
     8941 ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct ggcttcaatt
     9001 tcgcgtagct gcacgacatc gtctttatcc agaatgagcg ccgttgcttt accctgggcg
     9061 ttgtgggtga cgccttcctg cggcaccagt aatataaagc gcgcaaaatt attcaagata
     9121 tttttgaaaa tcattcaaga taggctatcg cgtgtgatag caaaaacatt caagataaaa
     9181 atgcttgatt attaagcaat caggaatggg agtattaagt ttcgtgtttc cagtaaaacc
     9241 agagagtatt gaaatagcta tcacctaact ttatcaggcg atagctggta gat
//
LOCUS       DAECNO010000133         9154 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.133, whole genome shotgun sequence.
ACCESSION   DAECNO010000133 DAECNO010000000
VERSION     DAECNO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.133"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..924
                     /locus_tag="KKO08_003565"
     CDS             <1..924
                     /locus_tag="KKO08_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6319666.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="KKO08_003566"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="KKO08_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBD6319667.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="KKO08_003567"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="KKO08_003567"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBD6319668.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="KKO08_003568"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="KKO08_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBD6319669.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="KKO08_003569"
     CDS             4447..4806
                     /locus_tag="KKO08_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBD6319670.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5934)
                     /gene="trmA"
                     /locus_tag="KKO08_003570"
     CDS             complement(4846..5934)
                     /gene="trmA"
                     /locus_tag="KKO08_003570"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBD6319671.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6048..6238
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6303..8147
                     /gene="btuB"
                     /locus_tag="KKO08_003571"
     CDS             6303..8147
                     /gene="btuB"
                     /locus_tag="KKO08_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBD6319672.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8092..8949
                     /gene="murI"
                     /locus_tag="KKO08_003572"
     CDS             8092..8949
                     /gene="murI"
                     /locus_tag="KKO08_003572"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBD6319673.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2094 a         2218 c         2469 g         2373 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcac ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgtc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tttacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gagaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt ggtaggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tatttcacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaagcaa cagaatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggt
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atctgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcttccgct tcctgacgcc actcatcatc cagcttttta tggtacagca gggaaaccac
     5581 cgcctgatta ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc gcagaacggg
     5641 attattacgc acacccgcaa tcatcgccgt catcaactgg ttgatgagtt cactggcggc
     5701 ggggaagcta tccacgcgga tgcggctttt ggtttgttga tcgaaaatga tgtgatacag
     5761 gtcatcgcca tcgtgccaga tgcggaactc cgcgcgcatc cggtaatgac tgaccggcga
     5821 gcgaaacact tccggaacca ggtcagaaaa cggtgccatc atactttgca aacgtaccac
     5881 tttttcggct aactgcgctt catactgttc tgttggaagg tgttcggggg tcatgatgta
     5941 tcctgaaaaa ttaaagtacg cggcgattgt agggattgct catcagatgt ccagatcttg
     6001 atgaattcct atttgtgagc tacgtctgga cagtaacttg ttacaacctg tagcatccac
     6061 ttgccggtcc tgtgagttaa tagggaatcc agtgcgaatc tagagctgac gcgcagcggt
     6121 aaggaaaggt gcgatgattg cgttatgcgg acactgccat ccggtgggaa gtcatcatct
     6181 cttagtatct tagatacccc tccaagcccg aagacctgcc ggccaacgtc gcatctggtt
     6241 ctcatcatcg cgtaatattg atgaaacctg cggcatcctt cttctattgt ggatgcttta
     6301 caatgattaa aaaagcttcg ctgctgacgg cgtgttccgt cacggcattt tccgcttggg
     6361 cacaggatac cagcccggat actctcgtcg ttactgctat ccgttttgaa cagccgcgca
     6421 gcactgtgct tgcaccaacc accgttgtga cccgtcagga tatcgaccgc tggcagtcga
     6481 cctcggtcaa tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt
     6541 caggtcagct ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg ctggtgttaa
     6601 ttgatggcgt ccgcctgaat ctggcggggg tgagtggttc tgccgacctt agccagttcc
     6661 ctattgcgct tgtccagcgt attgaatata tccgtggacc gcgctccgct gtttatggtt
     6721 ccgatgcaat aggcggggtg gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa
     6781 tttcagcagg gtggggaagc aatagttatc agaactatga tgtctctacg cagcaacaac
     6841 tgggggataa aacacgggta acgctgttgg gcgattatgc ccatactcat ggttatgatg
     6901 ttgttgccta tggtaatacc ggaacgcaag cgcagacaga taacgatggt tttttaagta
     6961 aaacgcttta tggggcgctg gagcataact ttactgatgc ctggagcggc tttgttcgcg
     7021 gctatggcta tgataaccgt accaattatg acgcgtatta ttctcccggt tcaccgttgc
     7081 tcgatacccg taaactctat agccaaagtt gggacgccgg gctgcgctat aacggcgaac
     7141 tgattaaatc acaactcatt accagctata gccatagcaa agattacaac tacgatcccc
     7201 attatggtcg ttatgattcg tcggcgacgc tcgatgagat gaagcaatac accgtccagt
     7261 gggcaaacaa tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac tggcagaaac
     7321 agactacgac gccgggtaca ggttatgttg aggatggata tgatcaacgt aataccggca
     7381 tctatctgac cgggctgcaa caagtcggcg attttacctt tgaaggcgca gcacgcagcg
     7441 acgataactc acagtttggt cgtcatggaa cctggcaaac cagcgccggt tgggaattca
     7501 tcgaaggtta tcgcttcatt gcttcctacg ggacatctta taaggcacca aatctggggc
     7561 aactgtatgg cttctacgga aatccgaatc tggacccgga gaaaagcaaa cagtgggaag
     7621 gcgcgtttga aggcttaacc gctggggtga actggcgtat ttccggatat cgtaacgatg
     7681 tcagtgactt gatcgattat gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc
     7741 ggattaaggg cgtcgaggcg acagccaatt ttgataccgg accactgacg catactgtga
     7801 gttatgatta tgtcgatgcg cgcaatgcga ttaccgacac gccgttgtta cgccgtgcta
     7861 aacagcaggt gaaataccag ctcgactggc agttgtatga cttcgactgg ggtattactt
     7921 atcagtattt aggcactcgc tatgataagg attactcatc ttatccttat caaaccgtta
     7981 aaatgggcgg tgtgagcttg tgggatcttg cggttgcgta tccggtcacc tctcacctga
     8041 cagttcgtgg taaaatagcc aacctgttcg acaaagatta tgagacagtc tatggctacc
     8101 aaactgcagg acgggaatac accttgtctg gcagctacac cttctgaacc acgtcccacc
     8161 gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct atgacgagat ccggcatctc
     8221 ttaccggatc tccattacat ttatgctttc gataacgtcg ctttcccgta tggcgaaaaa
     8281 agcgaagcgt ttattgttga gcgagtggtg gcaattgtca ccgcggtgca agaacgttat
     8341 ccccttgcgc tggctgtggt cgcttgcaac accgccagta ccgtttcact tcctgcatta
     8401 cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt
     8461 ctgacggcaa atggcattgt cggattactg gcaacccgcg gaacagttaa acgttcttat
     8521 actcatgagc tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct gggctcggca
     8581 gagatggttg agttggctga agcgaagctg catggcgaag atgtttctct ggatgcacta
     8641 aaacgtatcc tacgcccgtg gttaagaatg aaagagccgc cagataccgt tgtattgggt
     8701 tgcacccatt tccctctact acaagaagaa ctgttacaag tgctgccaga gggaacccgg
     8761 ctggtggatt ctggcgcagc gattgctcgc cgaacggcct ggttgttaga acatgaagcc
     8821 ccggatgcaa aatctgccga tgcgaatatt gccttttgta tggcaatgac gccagaagct
     8881 gaacaattat tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt
     8941 ttaggctgat ttggttgaat gttgcgcggt cggaaaatta ttttaaattt cctcttgtca
     9001 ggccggaata actccctata atgcgccacc actgacacgg aacaacggca aacacgccga
     9061 cgggtcagcg gggttctcct gagaattccg acagagaaag caaaaataaa tgcttgactc
     9121 tgtagcggga aagcgtatta tgcacacccc gcgc
//
LOCUS       DAECNO010000134         8997 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.134, whole genome shotgun sequence.
ACCESSION   DAECNO010000134 DAECNO010000000
VERSION     DAECNO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.134"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KKO08_003573"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="KKO08_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBD6319674.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KKO08_003574"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="KKO08_003574"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBD6319675.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KKO08_003575"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="KKO08_003575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBD6319676.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="KKO08_003576"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="KKO08_003576"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBD6319677.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4432..5556
                     /gene="dprA"
                     /locus_tag="KKO08_003577"
     CDS             4432..5556
                     /gene="dprA"
                     /locus_tag="KKO08_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBD6319678.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5528..6001
                     /locus_tag="KKO08_003578"
     CDS             5528..6001
                     /locus_tag="KKO08_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBD6319679.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6030..6572
                     /locus_tag="KKO08_003579"
     CDS             6030..6572
                     /locus_tag="KKO08_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBD6319680.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6577..7149
                     /gene="tsaC"
                     /locus_tag="KKO08_003580"
     CDS             6577..7149
                     /gene="tsaC"
                     /locus_tag="KKO08_003580"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBD6319681.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7154..7972
                     /gene="aroE"
                     /locus_tag="KKO08_003581"
     CDS             7154..7972
                     /gene="aroE"
                     /locus_tag="KKO08_003581"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBD6319682.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7969..8226
                     /locus_tag="KKO08_003582"
     CDS             7969..8226
                     /locus_tag="KKO08_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBD6319683.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8202..8756)
                     /locus_tag="KKO08_003583"
     CDS             complement(8202..8756)
                     /locus_tag="KKO08_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBD6319684.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2252 a         2183 c         2244 g         2318 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 tgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 actgaccaat cgcaaagatt gctaaggctt gcttatggca gggagataag gatggtcgat
     4441 acagaaattt ggctgcgttt aatgagcatc agcagcttgt acggcgatga tatggtccgt
     4501 atcgctcact ggctggcaaa acagtcgcat attgatgcgg ttgtattgca gcaaacaggg
     4561 cttacattgc ggcaggcaca acgctttctt tcatttccac gaaagagtat cgaaagctca
     4621 ctttgttggt tggagcaacc caaccatcat ttaatccctg cggacagcga attttatcct
     4681 cctcaacttc aggcgacgac agattacccc ggcgcactgt ttgttgaagg agaactgcac
     4741 gcgctgcatt catttcagct tgccgtagtg gggagtcggg cgcattcatg gtatggcgag
     4801 cgatgggggc gattattttg cgaaactctg gcgacgtgtg gagtgacaat tacgagtgga
     4861 ctggcgcgtg gaatcgatgg tgtggtgcat aaagcggcct tacaggtaaa tggcgtcagc
     4921 attgctgtat tggggaatgg acttaatacc attcatccgc gtcgccatgc ccgactggct
     4981 gccagtctgt ttgaacaggg tggcgctctc gtctcggaat ttcccctcga tgttccaccc
     5041 cttgcttaca atttcccacg aagaaatcgc attatcagtg gtctaagtaa aggtgtactg
     5101 gtggtggaag cggctttgcg cagtggttcg ctggtgacag cacgttgtgc gcttgagcag
     5161 gggcgtgaag tttttgcctt gccaggtcca atagggaatc cgggaagcga agggcctcac
     5221 tggttaataa aacaaggtgc gattcttgtg acggaaccgg aagaaattct ggaaaacttg
     5281 caatttggat tgcactggtt gccagacgcc cctgaaaatt cattttattc accagatcag
     5341 caagacgtgg cattgccatt tcctgagctc ctggctaacg taggagatga ggtaacacct
     5401 gttgacgtcg tcgctgaacg tgccggccaa cctgtgccag aggtagttac tcaactactc
     5461 gaactggagt tagcaggatg gatcgcagct gtacccggcg gctatgtccg attgaggagg
     5521 gcatgccatg ttcgacgtac taatgtattt gtttgaaacc tatattcaca ctgaagctga
     5581 gttgcgtgtg gatcaagaca aacttgaaca ggatcttacc gacgcaggtt ttgagcgaga
     5641 agatatctac aatgccctgc tatggctgga aaaacttgct gattatcagg aagggttggc
     5701 agaaccgatg caactggcct cggatcctct ctccatgcgt atttatacac cggaagagtg
     5761 tgaacgactg gatgccagct gccgtgggtt tctgcttttc cttgagcaga ttcaggtgct
     5821 caaccttgaa actcgtgaaa tggtgataga gcgagtgctc gcgctggata acgcagagtt
     5881 cgaactggat gatctgaaat gggtgatcct gatggtgttg ttcaacatcc cgggctgcga
     5941 aaatgcgtac cagcaaatgg aagaattact ctttgaagtg aatgaaggta tgctgcatta
     6001 atcatttctt taattcagca taagttgtta tggcaaaatc agcactgttc acggtgcgta
     6061 ataatgagtc ctgcccaaag tgcggggctg aactggttat tcgatccggg aaacacggtc
     6121 cgtttcttgg atgctcacag tatccggcgt gtgactacgt ccgtcctctg aaatcttcag
     6181 cggatggaca tattgtcaaa gttctggagg ggcaggtttg ccccgtatgt ggcgcaaatc
     6241 tggtattacg ccagggacgc tttggtatgt ttattggttg cagtaactac cctgaatgcg
     6301 aacataccga acttatcgat aaaccggacg aaacagcaat tacatgcccc caatgtcgga
     6361 cgggccatct ggtccagcgc cgctcccgtt atggcaaaac atttcactcc tgtgatcgct
     6421 acccggagtg tcaatttgcc attaacttca aaccaatagc tggagaatgc cctgagtgtc
     6481 attatccgct actcatcgaa aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta
     6541 aacaatgtgg aaagccggtt tcggcggaat aataacgtga ataataacct gcaaggagac
     6601 gctatcgcag ctgcgataga tgtcctcaat gaaaaacgtg tcatcgccta tccaacggaa
     6661 gccgttttcg gtgtcgggtg cgatcctgat agcgaaacag cagtgatgag actgttggag
     6721 ttaaaacagc gtccggttga taaggggctg attttaatcg cggcaaatta cgagcagctt
     6781 aaaccctata ttgatgacac catgttgact gacgtgcagc gtgaaaccat tttttcctgc
     6841 tggccaggtc ctgtcacctt tgtctttccc gcgcctgcga caacaccgcg ctggttgacg
     6901 ggccgctttg attcgcttgc tgtacgagtc accgaccatc cgttggtggt tgctttgtgc
     6961 caggcttatg ataaaccgct ggtttctacc agtgccaact tgagtggatt gccaccttgt
     7021 cgaacagtag acgaagttcg cgcacaattt ggcgcggcgt tcccggttgt gcctggtgaa
     7081 acgggggggc gtttaaatcc ttcagaaatc cgcgatgccc tgacgggtga actgtttcga
     7141 caggggtaac ataatggaaa cctatgctgt ttttggtaat ccgatagccc acagcaaatc
     7201 gccattcatt catcagcaat ttgctcagca actgaatatt gaacatccct atgggcgcgt
     7261 gttggcaccc atcaatgatt tcatcaacac actgaacgtt ttctttcgtg ctggtggtaa
     7321 aggtgcgaat gtgacggtgc cttttaaaga agaggctttt gccagagcgg atgagcttac
     7381 tgaacgggca gcgttggctg gtgcagttaa taccctcaag cggttagaag atggacgcct
     7441 gctgggtgac aataccgatg gtgtaggctt gttaagcgat ctggaacgtc tgtcttttat
     7501 ccgccctggt ttacgtattc tgcttatcgg cgctggtgga gcatctcgcg gcgtactact
     7561 gccacttctt tccctggact gtgcggtgac aatcactaat cggacggtat cccgcgcgga
     7621 agagttggct aaattgtttg cgcacactgg cagtattcag gcgttgggta tggacgaact
     7681 ggaaggtcat gagtttgatc tcattattaa tgcaacatcc agtggcatca gtggtgatat
     7741 tccggcgatc ccgtcatcgc tcattcatcc aggcatttat tgctatgaca tgttctatca
     7801 gaaaggaaaa actccttttc tggcatggtg tgagcagcga ggctcaaagc gtaatgctga
     7861 tggtttagga atgctggtgg cacaggcggc tcatgccttt cttctctggc acggtgttct
     7921 gcctgacgta gaaccagtta taaagcaatt gcaggaggaa ttgtccgcgt gaatcaggcc
     7981 atccagtttc cggacaggga agagtgggac gagaataaaa aatgtgtatg ttttcccgct
     8041 ctcgtgaatg gtatgcaact gacatgcgcg atctctggcg agagtctggc gtatcgcttt
     8101 actggagata cgccagaaca gtggttagcg agttttcgtc agcatcgctg ggacctggaa
     8161 gaagaagcgg aaaacttaat tcaggaacaa agtgaagatg atcaaggctg ggtctggtta
     8221 ccctgatcca gatattcgtc cttccatttc acgtaattat tcgcggaata gcgtaaccca
     8281 gccttctctt catcacttaa cgggcggatc tgtttgacgg ggctaccgag atacagatat
     8341 ccgctctcca gccgtttatt ttgtgggacc agactacccg caccaatcat cacatcatct
     8401 tctactattg cgccatcaag taaaattgag cccatcccaa ccaaaactcg attgccaatg
     8461 gtgcagccgt ggagcatcac cttgtgacca acagtgacat cttcgccaat ggttaatggg
     8521 ttgccatctg ggttgtacga ggatttatga gtgacatgca acatactgcc atcctggata
     8581 ttggtgcgtg ctccgatctg tacataatgt acatctccac gaatcacaac gagcggccag
     8641 atccccacat catcagccag acgaacgtca ccaatcacga cactgctatc gtcgatcatt
     8701 acgcgctgac cgatttgtgg aaaaagatcg cggtatgggc gtaaaacatc agacatactt
     8761 acctcagcaa taaatgattt actaatgact ttgggggcat tattggcctt gtgcaagtct
     8821 tttagtatgc aaaaaagcac cgttttgtgt gcgattgcag caaaaagggt gaaaaaacaa
     8881 caaacagaaa aaaagatcaa aaaaatactt gtgcaaaata ttgggatccc tataatgcgc
     8941 ctccgttgag acgacaacgt gaaacacttc acaggatggt cggaacaacg aagagaa
//
LOCUS       DAECNO010000135         8989 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.135, whole genome shotgun sequence.
ACCESSION   DAECNO010000135 DAECNO010000000
VERSION     DAECNO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.135"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            146..2330
                     /gene="espL4"
                     /locus_tag="KKO08_003584"
                     /pseudo
     CDS             146..2330
                     /gene="espL4"
                     /locus_tag="KKO08_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="KKO08_003585"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="KKO08_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="KKO08_003586"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="KKO08_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="KKO08_003587"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="KKO08_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="KKO08_003588"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="KKO08_003588"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBD6319685.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="KKO08_003589"
     CDS             8509..8952
                     /locus_tag="KKO08_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBD6319686.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2356 a         2168 c         2167 g         2298 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DAECNO010000136         8898 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.136, whole genome shotgun sequence.
ACCESSION   DAECNO010000136 DAECNO010000000
VERSION     DAECNO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.136"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..2887
                     /locus_tag="KKO08_003590"
                     /pseudo
     CDS             1..2887
                     /locus_tag="KKO08_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KKO08_003591"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="KKO08_003591"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBD6319687.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="KKO08_003592"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="KKO08_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KKO08_003593"
                     /pseudo
     CDS             complement(5343..7283)
                     /gene="yegI"
                     /locus_tag="KKO08_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7280..8041)
                     /locus_tag="KKO08_003594"
     CDS             complement(7280..8041)
                     /locus_tag="KKO08_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HBD6319688.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="KKO08_003595"
     CDS             complement(8038..8697)
                     /locus_tag="KKO08_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBD6319689.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2319 g         2048 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agaggttt
//
LOCUS       DAECNO010000137         8696 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.137, whole genome shotgun sequence.
ACCESSION   DAECNO010000137 DAECNO010000000
VERSION     DAECNO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.137"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..106)
                     /locus_tag="KKO08_003596"
     CDS             complement(<1..106)
                     /locus_tag="KKO08_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBD6319690.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="KKO08_003597"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="KKO08_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBD6319691.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1355..2005
                     /gene="csgD"
                     /locus_tag="KKO08_003598"
     CDS             1355..2005
                     /gene="csgD"
                     /locus_tag="KKO08_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBD6319692.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2010..2399
                     /gene="csgE"
                     /locus_tag="KKO08_003599"
     CDS             2010..2399
                     /gene="csgE"
                     /locus_tag="KKO08_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBD6319693.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2424..2840
                     /gene="csgF"
                     /locus_tag="KKO08_003600"
     CDS             2424..2840
                     /gene="csgF"
                     /locus_tag="KKO08_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBD6319694.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2867..3573
                     /locus_tag="KKO08_003601"
                     /pseudo
     CDS             2867..3573
                     /locus_tag="KKO08_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3706..4197)
                     /locus_tag="KKO08_003602"
     CDS             complement(3706..4197)
                     /locus_tag="KKO08_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBD6319695.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4299..4853)
                     /gene="ycdY"
                     /locus_tag="KKO08_003603"
     CDS             complement(4299..4853)
                     /gene="ycdY"
                     /locus_tag="KKO08_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBD6319696.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4877..5614)
                     /gene="ycdX"
                     /locus_tag="KKO08_003604"
     CDS             complement(4877..5614)
                     /gene="ycdX"
                     /locus_tag="KKO08_003604"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBD6319697.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5669..6607)
                     /gene="ghrA"
                     /locus_tag="KKO08_003605"
                     /pseudo
     CDS             complement(5669..6607)
                     /gene="ghrA"
                     /locus_tag="KKO08_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6842..6929
                     /locus_tag="KKO08_003606"
     tRNA            6842..6929
                     /locus_tag="KKO08_003606"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Ser,seq:gga)
     gene            complement(7122..8108)
                     /locus_tag="KKO08_003607"
     CDS             complement(7122..8108)
                     /locus_tag="KKO08_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319698.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(8105..8633)
                     /locus_tag="KKO08_003608"
                     /pseudo
     CDS             complement(8105..8633)
                     /locus_tag="KKO08_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         2476 a         2089 c         1858 g         2273 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatattttta
     1021 tatgtattat tagtaagtta gcaccatttg tatgattttt taaaattgtg caataaaaac
     1081 caaatgtaca acttttctat catttctaaa cttaataaaa ccttaatgtt aacattttaa
     1141 tataatgagt tacattttgt tacatgttta acacttgatt taagatttgt aatggctaga
     1201 ttgaaatcag atgtaatcca ttagttttat attttaccca tttagggctg atttattact
     1261 acacacagca gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc
     1321 aggtgtgcga tcaataaaaa agtggggttt catcatgttt aatgaagtcc atagtattca
     1381 tggtcataca ttattgttga tcactaaacc ttctttgcag gcgacagctc tcttgcagca
     1441 ccttaaacaa tcgctagcaa ttacaggaaa attacataat attcaacgtt ctctggacga
     1501 tatctcttca ggctctatta ttcttctgga tatgatggaa gcggataaaa agcttatcca
     1561 ttattggcag gatactttga gcaggaaaaa caacaatatc aaaatattgt tgctaaatac
     1621 gcctgaagat tacccgtacc gcgacattga aaactggcct catatcaacg gcgtttttta
     1681 tgccatggag gatcaagaac gtgttgtcaa tgggttgcaa ggcgtcctgc gtggcgaatg
     1741 ctactttacg caaaagcttg ccagttacct gattacacat tctggtaact atcgttataa
     1801 cagcacggaa tcagccctcc ttactcatcg ggaaaaagag atcctgaata agctgcgtat
     1861 cggcgcgtct aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac
     1921 gcatctttat aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg
     1981 ggcaaacgat aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg
     2041 cagaatttct gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgt
     2101 taactgacca tactgtttca tctattggcc atgattttta ccgagccttt agtgataaat
     2161 gggaaagtga ctatacgggt aacttaacga ttaatgaaag gcccagtgca cgatggggaa
     2221 gctggatcac aataacggtc aatcaggatg ttattttcca gactttttta ttcccgttga
     2281 aaagagactt cgagaaaact gtcgtctttg cactgattca aactgaagaa gcactaaatc
     2341 gtcgccagat aaatcaggcg ttattaagta cggacgattt ggcgcatgat gaattctaaa
     2401 taaaaaattg ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta ctcatgctta
     2461 tttcgccatt aagttgggct ggaaccatga ctttccagtt ccgtaatcca aactttggtg
     2521 gtaacccaaa taatggcgct tttttattaa atagcgctca ggcccaaaac tcttataaag
     2581 atccgagcta taacgatgac tttggtattg aaacaccctc agcgttagat aactttactc
     2641 aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg
     2701 gccgcatggt gaccaacgat tatattgttg atattgctaa ccgcgatggt caattgcagt
     2761 tgaacgtgac agatcgtaaa accggacaaa cctcgaccat ccaggtttcg ggtttacaaa
     2821 ataactcaac cgatttttaa gccccagctt cataaggaaa ataatcatgc agcgcttatt
     2881 tcttttggtt gccgtcatgt tactgagcgg atgcttaacc gccccgccta aagaagccgc
     2941 cagaccgaca ttaatgcctc gtgctcagag ctacaaagat ttgacccatc tgccagcgcc
     3001 gacgggtaag atctttgttt cggtatacaa cattcaggac gaaaccgggc aatttaaacc
     3061 ctacccggca agtaacttct ccactgctgt tccgcaaagc gccagggctt acaaaacctg
     3121 cttaacgagc gcaaggttat tcgtgcggca caagaaaacg gcacggttgc cattaataac
     3181 cgaatcccgc tgcaatcttt aacggcggca aatattatgg ttgaaggttc gattatcggt
     3241 tatgaaagca acgttaaatc gggcggtgtt ggggcaagat attttgggat cggtgcagac
     3301 acgcaatacc agctcgatca gattgccgtg aacctgcgtg tcgtcaatgt gagtaccggt
     3361 gagatcctct cctcggtgaa caccagtaag acgatacttt cctatgaagt tcaggccggg
     3421 gttttccgct ttattgacta ccagcgcttg ctggaagggg aagtgggtta cacctcaaac
     3481 gaacccgtta tgctgtgtct gatgtcggct atcgaaacag gggtcatttt cctgattaat
     3541 gatggtatcg accgtggtct gtgggatttg taaaataaag cagaacggca gaatgacatt
     3601 ctggtgaaat accgccatat gtcggttcca ccggaatcct gacagataaa aatgccggaa
     3661 ttaataatcc ggcatttact gtttaagcga aagcgtgagt gcccctcacg ctttattttt
     3721 gatttcctgc tcacggtgag ccgtctttgc actacgcgcc gccagccaca ggccgctgtt
     3781 tttcattgcg taaccaaata ccagcccaag cgccagcgaa ggtaacacca gtttccagtc
     3841 accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc cccggaacaa atgaaagcag
     3901 cagctgtttg gcctgaatac acatcagaaa cgcgacaata ccggttatga cataaccgag
     3961 aatttccaga tgtggtgcca gtgcactacc gtaaataatg accatcgccc acaccacgcc
     4021 actaagcagg gttgcagcgg agatcgccag ccctttcagc ccaccttgcg ggcaagcaaa
     4081 gtaggccgta cagcccagga agcccgccca gcttagtaag cccagggaaa cagccaccca
     4141 tccccagata ccggagagaa tgcctgttgt gattgcaata gagagaagta tgttcatgcc
     4201 gcgcatgata gcagaaaacg cggccatgaa tgagatcgag cacacatttt atgcaacaac
     4261 aaattatctg ttgcagttat gagtgacgat gatcacattt actcttcaga atcttcttcc
     4321 agctcgtccc acattgcact aatggcatca cgggttagcg gtgccatggt gcgccagaaa
     4381 ggcgtggttg catgggcctc cactttgccg aggaacgcac cacaccaggg taacagatac
     4441 tcactgaaca gtgtttccag tgcttcgctc tcatcttccg ttgactgatc ttccagccag
     4501 gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg tatccgccag tggcatccct
     4561 cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgt cctcaaccca tgcgctacga
     4621 tatggcggca cggcacattc atcgccgata aacaacgcat tgtaatcggc agagacttgc
     4681 gtcatatcac agcttttctg taaacgcgtc agtaactcat cctgctccag tggccagttc
     4741 gcagccagtt tcccctcacg aatcagagta aacagcggca ccagtaaagg atcttgcggt
     4801 tggcggtaat agagcgaacc cagcacacga cagaggatag aaaactcgtt cattaatata
     4861 tttccattac tagtaattaa aggtctgcaa attccgcaat cggtgccata ccgcgagatt
     4921 caaggaagtt cagtaagcgg cgcggagaaa cattcaaaat gcgctctagc ggaaaatcta
     4981 ccgcgtcgag gattttaaga cactcttcaa attcccccat ggtaaacgcg gtgtgagaat
     5041 ccgagcctaa tgccacccaa cctccagcat cacgtaccgc tgcagctacc gcacgacagt
     5101 tgtcttcact gcccttacgt gagtgtaaaa atgaggaatt attgatttcc agcgccacct
     5161 gatgtttcgc ggctgcttca gcaacggctt taacatctat ttcatatttc ggatttccgg
     5221 gatggcttat tatatgcaca ttgccgctgg cgatagtgga gatcatcgct tgtgtattgg
     5281 tcgctttgtc atgtggcgca aaaaccggct catgaaaacc ggcaataatc agatccagcg
     5341 agtcaaacat tttaccgctg cagtcaattt caccatcaac atttttaatg ttagcttcaa
     5401 tgccgcgcag gatccctacc ccatcaacca ctcgcggcca gatacgcatg ttaatgaagt
     5461 gccagtgatg cggcgcatct tccatatccg ggccatgatc ggtgatcgca aaaagtttaa
     5521 tgcccttttg tttggcctgg gcaatgtaat cactcaatgt gctatatgca tgtgtgctgg
     5581 caacggtatg catatgaagg tcgacgggat acataactct ctcctgtatt caattcccgc
     5641 caaggatagc aggaatcctg atgctttatt agtagccacg tgcgcggtcg acttgcccgc
     5701 agaccctctc ccctttttcg agctgggcaa tagtgcgaga aatgtactcc acagcttcag
     5761 cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac gcgtggatgt tgccagagcg
     5821 gactttcagg cggtaagggt tcacgattaa aaacatccag cattgcgcct ttaactttcc
     5881 cgctatccag cgccgcgagc aggtcatctt ccacaacatg aacaccacgc gccaggttga
     5941 ggagatacgc gccatcaggt aatttttcga gtaattgttg attaataatg ccgacggttt
     6001 caggggtatt cggtaacaaa ttaatcaata cccgacattg gctcagaaat gcagacagtt
     6061 cttcccgtcc ggcaaagctt tgcacgccag gccacgattt acgggttcga ctccagcaac
     6121 gcagcggaaa gcgctaggtt tgcagactct gagcaacttt acagcccagt acgcctgcgc
     6181 ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg cagcggttgc caatgcgaac
     6241 tattttgctg gatgcgataa tcgtcaaaac gtcgaaacca atgcagcacc tgactgacag
     6301 catattcctg catttgctcg cccataccgg tatcttccag gcgaaaaagt ggaacagaag
     6361 ggttcagcat ttcagggtgt gcctgtagct tgctcaaaat agaatcaaca ccggccccga
     6421 gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc aacaggagga tgccagacta
     6481 aaacataatc agcagagtca ttatctccgc ttttccatgc cctgactctt gcctgaggaa
     6541 tagctttgcg cagtgcctca atccaccatt gggtatcgaa cgttgggtga taaaagatga
     6601 tatccatact gactcccgaa aagcgttgtg cgaaatttat tcgcacttat cgttatgatc
     6661 tacaaaggcc accagcataa caaatccgtg gtcggtggca aaaaaagcag atttcgctta
     6721 ttaaaaccac acattgattg aatgtttgaa taaacgcgcg attttttcaa aaagtttgtt
     6781 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     6841 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     6901 cgggggttcg aatccccccc tcaccgccat atttaaagaa gagcgcgtac gaaagtacgg
     6961 gctttttttt cgtatattgt acacaccggg tgcccccgcc cacgtctgtt agggcgaggg
     7021 gaaattgtgc acatcaactg tctggttact cagtcaacaa tcaaaatgcg taagatagta
     7081 cagaacatat atgcgttatc tggtcacttc ctgtaacttc ctcacttatt aaaatgcccc
     7141 caacggaaat acatgtcaca tgccatcata aaatcgttat gccagaggaa tcgacaatgg
     7201 aaaaaatagt aaattaagca cggtacatag cagagtaaaa aataggcttc aaaccaaatc
     7261 cgccattcca caagcgttgc caataccgac accagcgtaa tccacatcat ttcaataacc
     7321 cgcaatatga ataaccgaag ccaaagcggc ttggcaaaaa aggtcgacaa cgtattttca
     7381 ctggagaaaa acagatcgat ttcctgcaaa aaaaacctgc ccagaaaagc gggccgcttg
     7441 gagaagcgca gaaaaaacag attaatcgcc aacactacgc cacaaagaat gagcaatgca
     7501 attatcgatt taaacgttaa ccactgcccc tctgtcacaa aagaatcact gttacgcaac
     7561 gggctgatag taagcaggtt aacaaccgtt gagatgcaca ccgaaagcag cacatagcga
     7621 gtcagacttt tatattttac ttttttcgct actgaaatag cgacctgctt gacataactg
     7681 gtgcggatca tccgaaacag cagaaagatg accaacggcc caagtaacga aatcaggaac
     7741 agcgcatata acgagggtag aagctggtat agcccccaac tcattgcact caacatgatt
     7801 aacgacagaa atcctgccac cagaggttgt gtaagaaata attctatttt gtggttctgc
     7861 tttttcttaa accacgaaaa atcgatattc ttcgtgacgc atttataagc ccagtacgcc
     7921 ttgagatcaa aaataaaatt atacagcacc agggccagaa tcccactaac tacaccaata
     7981 tcctctgatg gaaaagtaaa ccctgcattt ttccaataaa agcctatcag aataagtcca
     8041 atagcataaa tgtatatcag atttcgttta tcctgaaagc gaataaaagt taaataatca
     8101 ggaatcattt gaataccttt gcatcactaa atgctttacg acaatgcata actcgttgct
     8161 gatccacgcc agcaaaatca tttaacatat tccccaaata gggcttatta cttatttcaa
     8221 ggaaagctac cggggttggc atattcagaa tatgtatcga gagattcagt ccatttcacc
     8281 gtatgagtta agtgtaatga gagattttct ttgataattg acggagcgac ctcgcttttt
     8341 gctgttacgt tcatcagaac ctgatgctct ggcgaagcga tatccagtcc ggcaagataa
     8401 tcgcgcattg cctgaacgcc gtcttccatc aaacgcgtgt gccaggcacc gctcacaccg
     8461 agtttaaccg gttcgtatcc agcggccatc agcagcgtgg taaattcatt caacgcagcc
     8521 tgcgttcccc caataacctg ctggcgcggc gtgttatcac agctaatatc cagcgcaatg
     8581 cctgattccg taatcatcgt ctgcagttgt tcgcggttga tgcctttaac cgcctgatct
     8641 tacccagcaa tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgact
//
LOCUS       DAECNO010000138         8642 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.138, whole genome shotgun sequence.
ACCESSION   DAECNO010000138 DAECNO010000000
VERSION     DAECNO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.138"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="KKO08_003609"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="KKO08_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBD6319699.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="KKO08_003610"
     CDS             complement(1969..3180)
                     /gene="hemX"
                     /locus_tag="KKO08_003610"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBD6319700.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="KKO08_003611"
     CDS             complement(3202..3942)
                     /gene="hemD"
                     /locus_tag="KKO08_003611"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBD6319701.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVVWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="KKO08_003612"
     CDS             complement(3939..4880)
                     /gene="hemC"
                     /locus_tag="KKO08_003612"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBD6319702.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5267..7813
                     /gene="cyaA"
                     /locus_tag="KKO08_003613"
     CDS             5267..7813
                     /gene="cyaA"
                     /locus_tag="KKO08_003613"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBD6319703.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="KKO08_003614"
     CDS             complement(7853..8173)
                     /gene="cyaY"
                     /locus_tag="KKO08_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBD6319704.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8221..>8642)
                     /locus_tag="KKO08_003615"
     CDS             complement(8221..>8642)
                     /locus_tag="KKO08_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBD6319705.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1968 a         2235 c         2221 g         2218 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcca
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tgcaggtgca ggtttagctt ccgttgtccc cgctgccggt tgtgccagca ggttacgcac
     2101 gcgagtctgc atcagttttt ccagcatcgc ctggctttgc agggtttccg gaagatccat
     2161 cgagatattt tgctggctta actggtccac ctcgtcgagg aacgctttgg tggtggcatc
     2221 atcagtatcg tagtaagcac gtacccaggt ggagacgttc tccagcgcct ggcgataagt
     2281 ctcttcctga tgacgcggta cagcttgtgc tgcgaccagc aggcgagagc gaatattttc
     2341 gcgcagatag atatcctgat ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg
     2401 aatcgtaatg aagttgtcca taaagttctg ccagcttttt tgcagattga tacgccattc
     2461 gctgatggaa ctggaaagct cttcaccgtc tgaatccatc ggcgaaccat cgctgtcatt
     2521 atcggccaga cgcaggttat ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc
     2581 gtcataatcc acctgcgata ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg
     2641 aacggtaatc agactcgggt cattcatatc cgccaggctg gcgtctgcac ttttcagcaa
     2701 cgctgcagag gtcgtgacgt cctgatcgct ccacagcttc cgtccggcga gtttcaccag
     2761 aaaatcggcc tgagccagca gccaggtttt agcatcgctg ccggaaatgg tggcgatctt
     2821 ttgttggact tcatccaact gttttgccag cgtttcttgc tgacgattcg cctgcttaag
     2881 ctgcgcagct tgttgcttaa taatgccttc cagctcggct ttttggctct cctgggcttt
     2941 ttgcaatgcc gtcagttggt tagccagggc atcgctggtg gcggtctgat tgacggcctg
     3001 ttgtttaccc cagccataca aaccgacacc cgccgccaga gcaatagcga tagccaccgc
     3061 gctgagaatc aatgcagtat tgttcttact ctttttttct gttgcgacag gttgtgacgt
     3121 ggtgtccacg gcctccctgg tctcttcaac cacggcggag gttttttctt gttccgtcat
     3181 tatggcttcc tgttatgaga gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt
     3241 atcggcgacc ttaatgtctt gccagcccag ttcccgggcg agtttcgcca aacgctcact
     3301 gacgaccaat agtcgacagt gtagtaacca gtgctcacga taccattgtg ggatcagcga
     3361 ccagagttgc tgcaacattt caccgctggt aacaacgacc atcgtcacct cgcgggattg
     3421 ccagcgcatc gcttcttctg caccatcgta atggattgcg catcgttgat aacattcaca
     3481 aaaagtgacc tcagcaccgc gcaccgtcag ggtatcccca attagctcac gaccgccatt
     3541 gccacgtaat atcagcgcac gtttgcccgc aatattttgt aattcaggta attgtagcaa
     3601 gacttcgctg atttcccgat cctgtgggta gagaatcttc tgtccgctta cggtatgtag
     3661 cgccagtgcg gtggtgcgtc caatggcgaa ataagtaggt agttggggcc atttacgatc
     3721 ttgctgatgc agctgtgatt gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag
     3781 atcgctctcc cccagcgctg ccagttgatc agcaagttgc ggtaattgtc gacccggaga
     3841 aaactcaatc agcggaaaat gccagaccac ctgccccagt gtgcgcagac ggctcactaa
     3901 ctcttctcca gcgggagacg ggcgggtgac caggatactc atgccggggc ctctccgtta
     3961 tagacttcag cgaggatctc gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc
     4021 cccatttgtt cggcatcttg cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg
     4081 tccggcgcgc cgaccagcgc acatagccag atttcgccat caataagctc ggcgtagcta
     4141 ccaattggca cctgacatcc gccttcgaga cgggtattca tggcgcgttc tgcggtaacg
     4201 cgcagtgcag tttcgtggtg attcagcgcg gcaagcagct cgcgagtgcg tgtatcatca
     4261 aggcggcatt caataccgac tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt
     4321 aacgcggcgc gaatgcggga ctccagacct aaacgtttta gtccggctac ggcaagaatg
     4381 atggcatcgt attcgccgtt atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg
     4441 gagcggataa tcagatccgg gcggcgttca gccagttggc actggcgacg taaactggac
     4501 gtcccgacga tactgcctgc tggtaacgca tccagactgt catagttatt ggacacaaag
     4561 gcatcgcgag gatcttcacg ctcgcaaata gtgaccagcc ccagaccttg cgggaattca
     4621 accggcacat ctttcattga atgtacggcg atatcagcgc gattctccag gagcgcgact
     4681 tccagctctt ttacaaataa gccttttccg cctactttcg ccagcggcgt atcaagaatc
     4741 acatcgccgc gcgtcaccat cggtaccagt tcaacgacca ggcccggatg gctcgccatc
     4801 aacttgtctt tgacatagtg tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt
     4861 cttaaaacat tgtctaacat gcttgttacc gtcattatca tccgtggtcc atcctaacat
     4921 ccttgccaga gtgatgtcag tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat
     4981 gaggtctggc agtggatcct gacaggcgtt tcacgccgtc gtaataagga atttacagag
     5041 aataaacggt gctacacttg tatgtagcgc atctttcttt acggtcaatc agcaaggtgt
     5101 taaattgatc acgttttaga ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa
     5161 agtggtaacg gttacctttg acatacgaaa tatcccgaat gccgcgtgtt accgttgatg
     5221 ttggcggaat cacagtcatg acgggtagca aatcaggcga tacgtcttgt acctctatat
     5281 tgagactctg aaacagagac tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc
     5341 tgctatgggg cctgcattcc aacaggtcta cagtctactg ccgacattgt tgcactatca
     5401 ccatccgcta atgccgggtt accttgatgg taacgttccc aaaggcattt gcctttacac
     5461 gcctgatgaa actcaacgcc actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt
     5521 acaggatcca ccgaaaggtg agcttccaat tactggtgta tacaccatgg gcagcacctc
     5581 gtccgtaggg caaagttgtt cctctgacct ggatatctgg gtctgtcatc aatcctggct
     5641 cgatagcgaa gagcgccagt tactacaacg taaatgcagc ctgctggaaa gctgggccgc
     5701 ctcgctgggt gtggaagtca gcttcttcct gattgatgaa aaccgcttcc gtcataatga
     5761 aagcggcagc ctggggggcg aagattgtgg ctccacccag catatactgc tgcttgacga
     5821 attttatcgt actgccgtgc gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg
     5881 cgacgaagaa gagcattacg acgactatgt gatgacgctt tacgcgcagg gcgtgctgac
     5941 gccaaatgaa tggctggatc tcggtggctt aagctcgctt tctgctgaag agtactttgg
     6001 tgccagcctt tggcagctct acaagagtat cgattcccca tacaaagcgg tgctgaaaac
     6061 actgctgctg gaagcctatt cctgggaata cccgaaccca cgtctgctgg cgaaagatat
     6121 caaacagcgt ttgcacgacg gcgagattgt atcgtttggc ctcgatccat actgcatgat
     6181 gctggagcgt gttactgaat acctgacggc gattgaagat ttcacccgtc tggatttagt
     6241 acgtcgctgc ttctatttaa aagtgtgcga aaaactcagc cgtgaacgcg cctgtgtagg
     6301 ctggcgtcgc gcagtgttga gccagttagt gagcgagtgg ggttgggacg aagctcgtct
     6361 ggcaatgctc gataaccgtg ctaactggaa gattgatcag gtgcgtgagg cgcacaacga
     6421 gctgctcgac gcgatgatgc agagctaccg taatctgatc cgctttgcgc gtcgcaataa
     6481 ccttagcgtc tccgccagtc cgcaggatat cggcgtactg acgcgtaaac tgtatgccgc
     6541 gtttgaagca ttgccaggta aagtgacgct ggtaaacccg cagatttcac ccgatctctc
     6601 ggaaccgaat ctgaccttta tttatgtgcc gccgggccgg gctaaccgtt caggttggta
     6661 tctgtataac cgcgcgccaa atattgagtc gatcatcagc catcagccgc tggaatataa
     6721 ccgttacctg aataaactgg tggcgtgggc atggtttaac ggcctgctga cctcgcgcac
     6781 ccgtttgtat attaaaggta acggcattgt cgatttgcct aagttgcagg agatggtcgc
     6841 cgacgtgtcg caccatttcc cgctgcgctt acctgcaccg acaccgaagg cgctctacag
     6901 cccgtgtgag attcgccatc tggcgattat cgttaacctg gaatatgacc cgacagctgc
     6961 gttccgcaat caggtggtgc attttgattt ccgtaagctg gatgtcttca gctttggcga
     7021 gaatcaaaat tgcctggtag gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt
     7081 gcgtacgctg cacttcaacg gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg
     7141 caaaatgcat caggacgccg caccgccaga tagcgtggaa gtcttctgtt atagccagca
     7201 tctgcgcggc ttaatccgta ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg
     7261 cctttccagc acccgccagg aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac
     7321 ctggggcttg ttcttcgaac gcctgaatgt atcggtacag aagctggaga acgccatcga
     7381 gttttatggc gctatttcgc ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa
     7441 tcacgtcaaa ttaccggcgg tggtggacgg ctttgccagc gaagggatca tccagttctt
     7501 tttcgaagaa acgcaagacg agaatggctt taatatctac attctcgacg aaagcaaccg
     7561 ggttgaggtg tatcaccact gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg
     7621 cttctactcg tcatcgcacg accgcttcac ctacggctca agcttcatca acttcaacct
     7681 gccgcagttc tatcagattg tgaaggttga tggtcgcgaa caggtgattc cgttccgaac
     7741 aaaatcgatt ggcaacctgc cgcccgccaa tcaggatcac gatacgccgc tattacagca
     7801 gtatttttcg tgatgagcgt gccggaaagc gaggcttatc cggcatgcaa tcttagcgga
     7861 aactgactgt ttcacccgcc tgttgcgtcg ccgcctgttc cagcaaatcc cagaaggttt
     7921 cgccgctgcg atcgcaaatc cattcatcgc ctttcaggtc aaaatggtag ccgccctgtt
     7981 tggttgccag ccacacctgg tgcagcggct cctggcggtt aataatgatt ttgctgccgt
     8041 tttcaaaggt aatggtcagt acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat
     8101 ccctgtcgtc cagacgctct tcaatggtca gccacagttg atcagccagg cgatgaaatt
     8161 cactgtcgtt cattgttgta tcctgttttt aagtgatggc ggcagtatag cggcatgggg
     8221 tcagggcttc aaagtttgca cctctgctgc tgcgttccgg catgattcat ccgtcaccgg
     8281 aataatgatg tctctgtttt gaacttcacg tacgttctcc tctctgacac gagttttcgc
     8341 cgggcagggg gagggcgtaa tccttacctc tgtatcggtc catgtataaa cattgcgatc
     8401 gtcataagcg aagcgatcgt ttttagttag cgaaccgtta actggaataa tttgcaagct
     8461 ctttgaccgt aattggcgga taaagtaagc atgctgtttg ccgctattaa atgagcgatc
     8521 aatgaccagg tctggcgtat ccagcgggat tatctcaagc tggtcttcac caaatgtcga
     8581 taattttgtt acatacagcc gatctttata ttgcgcaata ttgccagtaa tcagatggaa
     8641 tt
//
LOCUS       DAECNO010000139         8502 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.139, whole genome shotgun sequence.
ACCESSION   DAECNO010000139 DAECNO010000000
VERSION     DAECNO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.139"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            47..400
                     /locus_tag="KKO08_003616"
     CDS             47..400
                     /locus_tag="KKO08_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBD6319706.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(437..2515)
                     /gene="flhA"
                     /locus_tag="KKO08_003617"
     CDS             complement(437..2515)
                     /gene="flhA"
                     /locus_tag="KKO08_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBD6319707.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2589..3599)
                     /gene="hypE"
                     /locus_tag="KKO08_003618"
     CDS             complement(2589..3599)
                     /gene="hypE"
                     /locus_tag="KKO08_003618"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBD6319708.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3596..4717)
                     /gene="hypD"
                     /locus_tag="KKO08_003619"
     CDS             complement(3596..4717)
                     /gene="hypD"
                     /locus_tag="KKO08_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBD6319709.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4717..4989)
                     /gene="hypC"
                     /locus_tag="KKO08_003620"
     CDS             complement(4717..4989)
                     /gene="hypC"
                     /locus_tag="KKO08_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBD6319710.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4980..5852)
                     /gene="hypB"
                     /locus_tag="KKO08_003621"
     CDS             complement(4980..5852)
                     /gene="hypB"
                     /locus_tag="KKO08_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBD6319711.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5856..6206)
                     /gene="hypA"
                     /locus_tag="KKO08_003622"
     CDS             complement(5856..6206)
                     /gene="hypA"
                     /locus_tag="KKO08_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBD6319712.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6418..6879
                     /gene="hycA"
                     /locus_tag="KKO08_003623"
     CDS             6418..6879
                     /gene="hycA"
                     /locus_tag="KKO08_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBD6319713.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7004..7615
                     /gene="hycB"
                     /locus_tag="KKO08_003624"
     CDS             7004..7615
                     /gene="hycB"
                     /locus_tag="KKO08_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBD6319714.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7652..>8502
                     /locus_tag="KKO08_003625"
     CDS             7652..>8502
                     /locus_tag="KKO08_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063859.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBD6319715.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGI"
BASE COUNT         1906 a         2308 c         2289 g         1999 t
ORIGIN      
        1 aagattaaga tgtaatataa atgcatctta atgaggtgga ggcagcatgt ccggcaagcg
       61 tatctctcgt gaaaaactga cgattaaaaa aatgatcgat ctttatcaag cgaaatgccc
      121 gcaggcgtca gcggagccgg agcattacga ggcgttgttt gtttacgcgc aaaagcggct
      181 ggataaatgt gtgttcggcg aagagaaacc agcctgtaag cagtgtccgg tgcactgtta
      241 ccagcctgca aagcgtgaag agatgaaaca gattatgcgt tgggccgggc cgagaatgct
      301 gtggcgtcat ccgatcttaa ccgtgcgtca tctcatcgat gacaagcgtc cggtgccaga
      361 actgccggaa aaatatcgtc cgaaaaaacc tcgtgaatag tatgccggac aggcagatct
      421 gtccggcaat ttgcagttaa atcaatgccg atttatcaat tcccagccgc ttcatccgtg
      481 acagcagggt cgtgcgtttc agccccagac gttgcgcagc gcctttaggc ccggcaacca
      541 cgctgttggt ttctttcagc acgcgcacaa tcaactgata ttcatcttcg ccctcctggg
      601 cgacaaccgt tgcggcaggc ggcgtttcag gctccggtaa agcaatatct ggcaatgaca
      661 gctgcagcac gttgccgcgt gttagcaata ctgcgcgctc aatgacgttt tccagttcgc
      721 gtacgttacc cggccactcc atgttgctca aggtgcgcag cgtctcggca ggaatgctgt
      781 cgatattgcg ccccagacga cgggcaattt tgaaggtaaa ggctttcgcc agcagcggaa
      841 tatcttccgg acgctcgcgt agtggcggca ggtgaatcgg gaatacgttc aggcggtaat
      901 agagatcgct acggaactct cggtcggcga ccattttttt cagatcgcgg ttagtcgcgg
      961 caattaaacg tacgtccgtc tgaatgattt tgttgctgcc gagacgttca aactcctgtt
     1021 cctgcaatac acgcagcaac ttcggctgta actccagtgg catatcgccc acttcgtcga
     1081 ggaacagaga gcttttatcc gccagttcaa aacgaccaat gcgctgggcg ctggcaccgg
     1141 taaaagcccc acgctcatga ccaaacagat cgctttccag caatccggca ggcatcgccg
     1201 cgcagttcat tttgaccatg cggcgattat tacgcccact gagattatgg atcgcacggg
     1261 caatcagctc tttacccgag ccagtttcac cgaggatcag cacggtactg tcactttgcg
     1321 ccaccatttc aacttgttta agcacgctgt acatggcttc gctgcggcca ataatctcgc
     1381 caaattcact atcaacattg ttgagctgct cggtcagggc gaggttttca tcaaccagcc
     1441 gttctttcag acgatggatt tcctgatagg cgagggcgtt atcgacagcg attgccacac
     1501 gttcggcaat ctggcgcagc aaattcagat tggtagtggt aaacactttc tcttcgcatt
     1561 gcgccagttt cagcacgccc agcatggtgt cgccagacat cagcggtaac aggcacaagg
     1621 tttgaatctg gttgccccag gtgtcgaaca acatgcgttc atagggggct aaatcgtccc
     1681 gctcgtagag attgatcagc agcatctctt tacttttgaa cacgcgttcg gtgagggttc
     1741 cggcttcatc gacttcgctc tgttcgtggg cgggatgttg tttatcaaga tagtgagtgg
     1801 agtagatgtt gagtttgttt ttacggtggc tgcgtaagac aatgctgata tcgtcaatgt
     1861 cgaaatagta atggatttct ttggcgactt cgctgaccag ttcgtccata tccaggcggg
     1921 aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt atcgcgctcc cggcataaca
     1981 actcatagtc gacattgttg acgacgcggc tctggatttg ttcggtgacg acagaaacga
     2041 tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct ccagggacgc tcgtcataac
     2101 gaataaattc acagccacca aaaatatgcc ctcccgccgc cagtggcatc aggcaatagt
     2161 gaccaaattt ggggtatagc ccacctgcgg ccagctgcgg ccaggtttca caaaattctt
     2221 cgtaactgca atgcagcgta tcagggcgcg acaaaatgct gcgtaccgga ccgtgtgcca
     2281 gaacagtttc gtcttcatat ttaatggggg tgtctttttc acgcgacgcg taataagacg
     2341 cacgttgagt ctgcgcttgc cacaacacaa tagccgcgtt gtcggcgagc gcagaacgct
     2401 ttaccagttg cgaaagagcc tcacacagcg aggccagatc gggctgctgc aatagtgtcc
     2461 gagtgatgtc gaacaaccct tgctgtccga gatcactcat cggtgtatat gacattttgt
     2521 tcttccagga aagcaccgca aagcggtgca aaaggtcatt gactaactat aggagattta
     2581 gaattttatt agcatatacg cggaagcggt tcggcgtgtg gtaaatcgag ggttcgtttc
     2641 acgccataca gaccggcaag acgaacacct ttacgttcca ccacttcacc aatcagcgcc
     2701 gcgtctttcc ccagtggatg ggaatgtaac gctgccagca cttgctctgc cgcgttgcgt
     2761 tcaacggcga tcaccagttt gccttcgttg gcaaagttca gggcgtccag tcccagcaat
     2821 tcgcaaacgc cgcgcacggc aggtttaacc ggcagcgctg attcagaaat ttcaatacca
     2881 caaccgcagg ctgccgcgaa ctcatgaacc accgcgttta caccaccacg ggtggcatca
     2941 cgcagcgctt tcacgccggg aatgtcacgc agcgtctgaa taagcggcgt cagcaccgcg
     3001 cagtcgctga ccagttcgcc atccagcccc agctgctcac gcaggttaag gatagtcgcc
     3061 ccgtggtcgc cgagtgtacc gctaaccagc aatacatcgc ctgcggttag cgtctgtgcg
     3121 ccccagtgaa tattcgccgg aattgcgccc atgccagcgg tgttgataaa cagtttatct
     3181 gccgcgccgc gctgcaccac tttagtatcg ccagtaacga tggcaatgcc tgccgtgcgg
     3241 gcggtttctg ccatgctggt cactacggct ttcagtgtct ccatcggcaa tccttcttcg
     3301 aggataaagc cacaggagag atagcgcgga atagcgccac tgcccgcaac gtcattcgcg
     3361 gtgccgcaaa tcgccagctt gccgatatta ccgccaggaa agaacagcgg gtcaatgacg
     3421 taactgtcgg tggagaacgc cagacggtcg ccttccgcta ccagctgcgc cagatcaaga
     3481 cgtgcctgat cttcctgctc tgccagccac gggttggcaa aggcttccat aaacaggctg
     3541 ttgattaatt gctgcatcgc ctggccgccg ctgccgtggg cgagttggat attattcacg
     3601 cttcactctc ctgctgacga tactgatacc acgcggcgca cgctccttcg gaggagacca
     3661 tcagtgcacc aaatgcggtt tgaggattac aggtgttacc aaacagcggg cattgatgcg
     3721 gcttacattt gcccgttaat acctcaccac aacgcgcgcg cgggtcatcg cagacctgct
     3781 gcggtgccgg gcggaaatgt gcttcggcat cgaatcgttg ataatccggc gtcaggtgca
     3841 cgccagaaga ttcaatcacg cctaagccgc gccattcgct gtcgccgttg acacagaaca
     3901 catcggcaat cgcctgttgc gccagcaggt taccggcatc cggtaccacc cgacgatact
     3961 gattctctac cttgctgtgg gccgctattt tctgctccac cagcatgacc acgccttgca
     4021 gtagatcaag gggttcgaaa ccagcgacca ccagcggacg atgaaaatcg ctggcgataa
     4081 aattataggc atcagtgccg ataaccatac taacgtggcc cggcgcgagg aacgcgtcga
     4141 taccgttatc cggctgttcc agcaaactgc gcagcgtcgg gataagcgta atgtgctggc
     4201 agaagaagta aaaattctgc acatcacgcg ctttcgcctg ttgcagagtg atggcggtgg
     4261 tcggcatggt ggtttcaaaa cctaagccga agaacaccac tttgcgggtt ggattctcct
     4321 gcgccagttt caacgcatcc atcggcgagt aaacgatgcg cacatcggca ccgcgtgctt
     4381 ttgcctgcaa cagcgatccc tgtttccctg gtacgcgcat ggcgtcgcca aaggtacaga
     4441 agatgacttc cggatggctg gcaatctcca cgcaggtgtc gattctaccc atcggcagta
     4501 cgcacaccgg gcaacccgga ccgtggataa actcaacgtt ttcaggcagt aactggtcga
     4561 ggccgaattt aaaaatggcg tgggtatgac cgccacacac ttccataatc cgcagagggc
     4621 gttcggcggt gtaagagaga tgtgaagcac gttcgcgcag atgctcaatt aactgcatca
     4681 cctgttccgg cgcgcgatat tcatcaacaa aacgcattat ttttcctcgc catacaacag
     4741 cgcgccgaca tccggctcaa cgtcaaacat gttttgtaag gcgtcgagag tgtcgcgtgc
     4801 ttcggcttca ttaattacgc tcatggcaaa gccaacgtga accagtaccc actggcccac
     4861 gcgcggctga ccgttttcat cgcagctgcc gactaacgtt aaatcgacat cgcgctgaat
     4921 gccgcagacg tcgactttcg cctggttgcc gtcaatggtg cggatctggc cgggaacgcc
     4981 tatgcacatc gctgtgtctc cagccagttc agccactggt ccatcccttc gccgctggtg
     5041 gcggaaataa ggatgatttc aatttctgga ttgacttcgc gggcgcaggc gatgcacttc
     5101 tcaacgtcaa agttgagata cggcaacagg tcaactttgt tgagcagcat cagcgaggcg
     5161 gcggcaaaca tatgcggata tttcagcggt ttgtcttcac cttcggtaac ggaaagcacc
     5221 gctactttgt gtttttcacc gagatcgaag ctggccgggc atacgaggtt gccaacgttt
     5281 tcgataaaca gaataccgtt atcgtccagt ggcagacgcg gtgcggcgtc ggcaatcatc
     5341 tgtgcgtcaa gatggcagcc tttaccggtg ttcacctgaa tcgctggtgt accggtagcg
     5401 cgaatgcgtg cggcatcgtt cacggtttgc tggtcgcctt caataaccgc gcacggaacg
     5461 ctgtctttca ggcgcattaa ggtttccgtc agcagggtgg ttttaccgga accagggctg
     5521 gaaaccaggt tgagcaccag ttgcttgcgg gcagcaaagc gcgcgcggtt gcgttcagcc
     5581 agacggttgt ttttgtccag cacgtcaatt tcgacttcca gcatccgacg ctggctcatg
     5641 cccggtgtgt gagtgcccgc ttcgccatga ccgtaatgca ggtcgccttc ttcagtctgg
     5701 ctgggggtaa attcaggcgc tttaatgccg gtgattttca tctgcgggcg tgccgccggg
     5761 gcaaatggcg cgctacgaaa cgcggaatga gggttatgtt catcaccctc gatatacagg
     5821 ttgccttcac cgcaaccgca tgttgtacac atcgctcact cctggtctat ttctatccgc
     5881 cgaatctgta aaccgtcgtc tgccacaatc tgtagcatgt caccatgaca ctgtggacag
     5941 cggcggacgc gctgggtcag tagcgtcaca tactgttggc atgtttcaca ccagcattcg
     6001 gcctcttgtt cttcgaggtg cagtttacaa ccttccgcca cgctgccgcg gcaaaccaga
     6061 tcaaaacaaa aggcaagaga gctggtttcg acacaagaaa atgcgccaat tttgagccag
     6121 accccagtta cgcgttttgc gccgtgtttt gcggcctgct gttcgatcaa ttccagtgcc
     6181 cgttggcaga gggttatttc gtgcatatcg cctcccatta actattgcca gctacaagca
     6241 ataattgcgc cagtgttgat tatccctgcg gtgaataatg tcgatgatgt cgaaatgaca
     6301 cgtcgacacg gcgacgaaat tcatctttag cttaaaaatc tctttaataa caataaatta
     6361 aaagttggca caaaaaatgc ttaaagctgg catctctgtt aaacgagtaa cctgacaatg
     6421 actatttggg aaataagcga gaaagccgat tacatcgcac agcggcatcg tcgcctacag
     6481 gaccagtggc acatctactg caattcgctg gttcagggga tcacgttatc gaaagcgcgc
     6541 ctgtatcacg ccatgagctg cgcgccggac aaagaactct gtttcgtcct ttttgaacat
     6601 tttcgtattt acgtcaccct ggcggatggc tttaacagcc acaccatcga gtattacgtc
     6661 gaaacaaaag atggcgaaga caaacagcgg attgcgcagg cgcaactgag cattgacggc
     6721 atgattgatg gcaaggtcaa catccgcgat cgcgaacagg ttctggaaca ctatctcgaa
     6781 aaaatcgctg gcgtttacga cagcttatac accgccattg aaaacaatgt gccggtgaat
     6841 ttaagccaac tggtaaaggg acaaagcccg gcagcatgag ctgaggcttt gcccgttttg
     6901 caggcgttac gcctgtttgg ggatgggcgt gtcgatgagt gtcgaaaatg acatttcatc
     6961 ggcatgtttt cgtcaaaaat gacaatcacc tgaggaatgc ctggtgaatc gttttgtaat
     7021 tgccgactcc acgctctgta tcggctgcca cacctgtgag gccgcctgtt cagagacgca
     7081 tcgccagcac ggcctgcaat caatgccgcg cctgagagtg atgctcaatg aaaaagaatc
     7141 tgcgccgcag ctctgtcacc actgtgaaga tgcaccctgc gcggtggtct gcccggttaa
     7201 cgccatcacc cgcgttgatg gggccgtgca gttgaatgaa agtctgtgcg taagctgcaa
     7261 actgtgcggc atcgcctgcc cgtttggcgc aattgaattt tccggcagcc gtccgctgga
     7321 tattccggca aacgccaata ccccgaaagc gccaccggca ccgcctgctc cggcgcgtgt
     7381 cagcacattg cttgactggg tgccaggtat tcgcgcgatc gccgtcaaat gtgacctttg
     7441 tagctttgat gaacaaggtc cggcctgtgt gcggatgtgc ccgactaaag ccctgcatct
     7501 ggtggataac accgatatcg cccgcgtcag caaacgtaag cgtgagctga cctttaacac
     7561 ggactttggc gatctcacct tgtttcagca ggctcaaagt ggagaggcta aatgagcgca
     7621 atttccctga tcaatagcgg cgtggcatgg tttgtcgccg ccgctgttct ggcatttctc
     7681 ttttcaaaaa gcgctaagtg gctggatagc cggaattggc ggcgcggttg gtagtctgta
     7741 tacggcagcc gcgggcttca ctgtactgac tggcgcggtt ggcgtgagcg gtgcgctgtc
     7801 gctggtaagc tacgatgtgc aaatctcccc gcttaacgcg atttggctga ttaccctcgg
     7861 cctttgcggt ctgtttgtca gcctctataa cattgactgg catcgccacg cgcaggtgaa
     7921 gtgcaacggc ttgcagatca atatgttgat ggctgccgcc gtctgcgccg tcatcgccag
     7981 caacctcggc atgttcgtgg taatggccga agtcatggcc ttgtgtgcgg tgttcctcac
     8041 cagcaacagc aaagagggca aactgtggtt tgcgctgggg cgtcttggca ctctgctgct
     8101 ggtgattgct tgctggctgc tgtggcagcg ttatggcacg ctggatctgc gcctgctgga
     8161 tatgcgtatg caacagctgc cgctcggttc cgatatctgg ctgctcggtg tgattggctt
     8221 tggcctgctg gctgggatta ttccgctgca cggctgggtg ccgcaggcac atgctaacgc
     8281 ctctgcgcca gctgccgcgt tgttttctac ggtagtcatg aaaattggcc tgctgggcat
     8341 gttaaccctg tcactgctgg gcggtaatgc accgctgtgg tgggggatcg cgctgctggt
     8401 gctcggcatg atcaccgcgt tcgtcggtgg tctgtatgcg ttgatggaac acaacatcca
     8461 gcgcctgctg gcttaccaca ccctggaaaa tatcggcatt at
//
LOCUS       DAECNO010000140         8464 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.140, whole genome shotgun sequence.
ACCESSION   DAECNO010000140 DAECNO010000000
VERSION     DAECNO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.140"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            34..729
                     /gene="aqpZ"
                     /locus_tag="KKO08_003626"
     CDS             34..729
                     /gene="aqpZ"
                     /locus_tag="KKO08_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBD6319716.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1186..2085
                     /gene="lysO"
                     /locus_tag="KKO08_003627"
     CDS             1186..2085
                     /gene="lysO"
                     /locus_tag="KKO08_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBD6319717.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            2229..3881
                     /gene="hcp"
                     /locus_tag="KKO08_003628"
                     /gene_synonym="priS"
     CDS             2229..3881
                     /gene="hcp"
                     /locus_tag="KKO08_003628"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBD6319718.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            3893..4861
                     /gene="hcr"
                     /locus_tag="KKO08_003629"
     CDS             3893..4861
                     /gene="hcr"
                     /locus_tag="KKO08_003629"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBD6319719.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4994..6712
                     /gene="poxB"
                     /locus_tag="KKO08_003630"
     CDS             4994..6712
                     /gene="poxB"
                     /locus_tag="KKO08_003630"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBD6319720.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6749..7750
                     /gene="ltaE"
                     /locus_tag="KKO08_003631"
     CDS             6749..7750
                     /gene="ltaE"
                     /locus_tag="KKO08_003631"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBD6319721.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7761..>8464
                     /locus_tag="KKO08_003632"
     CDS             7761..>8464
                     /locus_tag="KKO08_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBD6319722.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIP"
BASE COUNT         1853 a         2198 c         2326 g         2087 t
ORIGIN      
        1 ttttcacagg gtcaattttt aattgaggtg gatatgttca gaaaattagc agctgaatgt
       61 tttggtactt tctggcttgt ttttggtggc tgtggtagtg ctgtactggc cgcaggcttc
      121 ccggaattag gcattggttt tgccggcgtg gcgctggcat ttggtttgac cgttctgacg
      181 atggccttcg ctgttggtca tatttctggt ggtcatttta acccggcggt cactattggt
      241 ttatgggctg gcggacgttt tccggcaaaa gaagtcgttg gctacgtaat tgcccaggtt
      301 gtcggcggta ttgttgcagc ggcgctgctg tatttaattg ccagtggtaa aacgggtttt
      361 gacgcggcag ccagcggttt tgcttctaac ggttatggcg agcattcacc aggcggttat
      421 tccatgcttt ccgcgctggt agttgaactg gtattgagtg cgggcttcct gttagtgatc
      481 cacggtgcaa ccgacaaatt cgctccggca ggttttgcgc cgatcgctat tggtctggcc
      541 ttaaccctga ttcacttaat cagtattccg gtgactaaca cttctgttaa cccggcgcgc
      601 agcaccgcgg ttgctatctt ccagggcggc tgggcattag aacaactgtg gttcttctgg
      661 gtggtgccaa ttgtcggcgg cattatcggt ggtctgattt accggactct gctggaaaag
      721 cgtgattaat tagcattggc attttatctg ccctggttgt tttatcttcg acattacaaa
      781 ccgcacgggt attggtggcg tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc
      841 acatggatgt gggctgaggg cgcgtcttac aggacgttac ctcgcgcccg acccggttgc
      901 cgaaggggat aagctgaagg taccgcgcag cggcgatttt gttcgccaga gcccgggggt
      961 gcagggggcg gcggcgactg gctgccccct gcgcgctccc tccgccagtg gctttatatg
     1021 gcattgcaca tgaaaggagc gcaacaatgc taaacactca gataggatta aaaatccccc
     1081 tctgatgcat aacatttcct tttaattgca actttactcg cccttccgct ttcagtagtg
     1141 tgtcatcgcg catttcgact tcttctttgc aaggactgga ttttcatgtt ttctgggctg
     1201 ttaatcattc tggttcccct gattgtgggt tacctcattc cgcttcgcca acaagctgcg
     1261 ttaaaagcta ttaatcagct attaagctgg atggtttacc ttattctctt ttttatgggt
     1321 atcagtctgg cgtttctcga taacctcgcc agtaacctgt tggcgattct gcattattct
     1381 gccgtcagta ttaccgttat tttactgtgt aatattgccg ccctgatgtg gctggagcga
     1441 ggcctgccgt ggcgcaacca ccatcagcaa gaaaaactcc cgtcgcgtat tgcgatggcg
     1501 ctggagtcgc taaaactgtg cggcgtagta gtgattggtt ttgccattgg tctgagtgga
     1561 ctggctttct tacaacacgc gaccgaagcc agtgaataca cgttaatttt gctacttttc
     1621 ctcgttggta ttcagttgcg caataatggc atgaccttaa agcagattgt ccttaatcgc
     1681 cggggaatga ttgtcgccgt ggtggtggtt gtcagttcat taattggtgg tttaattaac
     1741 gcctttattc ttgatctccc catcaatacc gcgctggcaa tggcctccgg tttcggctgg
     1801 tattctcttt ccggtatttt attgaccgaa tcttttggtc cggtaatcgg gagcgcggcg
     1861 ttttttaatg atctggcccg tgaactgatt gctattatgt tgatccctgg gctgattcgc
     1921 cgcagccgct ctactgcact gggcttatgc ggtgccacat caatggattt caccctgccc
     1981 gttcttcaac gtactggcgg gctggatatg gtcccggcgg caattgttca cggttttatt
     2041 cttagcctgt tagtgccgat cctcatcgcc tttttctctg cgtaatacct ctctggcggt
     2101 agttccctgc cgccaaaatt gcgctaaatc aatctccctt aaagttgcat gaaaaatccc
     2161 ttttatcccc gcgttaagcg tcttaccctt aaacatgtat attaaatata actttaaaag
     2221 gtgtgatcat gttttgtgtg caatgtgaac aaactatccg tactccggca gaaaacggct
     2281 gctcatacgc gcaggggatg tgtggtaaaa cggcggaaac ttctgacctt caggatttac
     2341 tcatcgcggc gctgcaaggg ctttctgcct gggcggtaaa agcgcgtgaa tacggcatca
     2401 tcaaccacga tgttgacagc ttcgcgccac gtgccttttt ctcaaccctg accaacgtta
     2461 acttcgattc tccgcgtatt gtcggctacg ctcgtgaagc gattgccttg cgcgaggcgc
     2521 tgaaagcaca atgcctggct gtagatgcca acgcgcgcgt cgataacccg atggctgacc
     2581 tgcaactggt gagcgatgat ctcggcgagc tgcaacgtca ggcagcagaa tttaccccta
     2641 acaaagataa agcggcgatt ggcgaaaaca ttctcggcct gcgtctgctg tgcctgtatg
     2701 gcctgaaagg tgcggcggcc tatatggaac acgcgcatgt tctcggtcaa tacgacaacg
     2761 atatttatgc ccagtaccat aaaatcatgg cgtggctggg gacctggcct gccgatatga
     2821 acgcgcttct tgagtgttca atggaaatcg gccagatgaa cttcaaagtg atgagcattc
     2881 tggatgcagg cgaaaccggt aaatacggtc acccgacgcc aacccaggtc aacgtcaaag
     2941 cgacggcggg taaatgcatt ctgatttccg gtcacgatct caaagatctc tacaacctgc
     3001 tggaacagac cgaaggcacg ggcgttaatg tctacaccca cggcgaaatg ctgcctgcgc
     3061 atggctaccc ggagctgcgt aaattcaagc atctggtcgg taactacggc agcggctggc
     3121 agaatcagca agtggagttc gctcgtttcc ctggccccat cgtgatgacc tcgaactgca
     3181 tcatcgaccc aaccgtaggc gcttatgacg atcgtatctg gacccgcagc atcgttggct
     3241 ggcctggcgt gcgtcatctg gatggcgaag atttctctgc ggtcatcgcc caggcgcaac
     3301 aaatggcggg cttcccgtac agcgaaattc ctcacctgat caccgtgggc tttggtcgcc
     3361 agacgctgct tggtgcagcc gatacgctga ttgatctggt tagccgtgaa aaactgcgtc
     3421 atatcttcct gcttggtggc tgtgacggcg cacgcggcga gcgccactac ttcaccgatt
     3481 tcgccaccag cgtgccggat gactgcctga tcctgaccct cgcctgtggt aaatatcgct
     3541 ttaacaaact tgagtttggc gatatcgaag gtctgccgcg tctggtagat gcaggtcaat
     3601 gtaacgatgc ttactcagca attattctgg ctgtcactct ggcagagaaa ctgggctgtg
     3661 gtgtgaacga tctgccgctg tcgctggtgc tctcctggtt tgaacagaaa gcgattgtca
     3721 ttctgctgac gctgctttct ctgggcgtga aaaatatcgt caccggcccg actgcacctg
     3781 gtttcctgac accagacctg ctggcggtgc tgaacgagaa attcggcctg cgttctatca
     3841 ccactgttga agaagacatg aagcaactgt tgaacgcgta aggagtttgt cgatgacgat
     3901 gccaacgaat caatgcccgt ggcggataca ggttcatcac attacgcaag aaacgccgga
     3961 tgtgtggacg atttccctga tttgccacga tcactaccca tatcgcgccg ggcaatatgc
     4021 actggtcagc gtgcgaaact cagcggaaac gctgcgtgct tacaccattt cctccacgcc
     4081 aggcgtgagt gaatatatca ccctgaccgt gcggcggatt gatgacggtg tcggctccca
     4141 gtggctgacg cgcgatgtaa aacgcggtga ttatctctgg ctttcggacg cgatggggga
     4201 atttacctgc gacgataaag cagaagataa attcctgttg ctggcggcag gctgcggcgt
     4261 cacgccgatt atgtcgatgc gtcgctggct tgcgaagaac cgtccacagg ccgatgtgcg
     4321 ggtgatctac aacgtgcgta cgccgcagga tgttattttc gccgatgagt ggcgtaacta
     4381 tccggtaacg ctggtggcag aaaataacgt taccgaaggc tttatcgctg gtcgtctcac
     4441 tcgcgaactg ctggcagatg tacctgactt agcttcacgt accgtgatga cctgcggccc
     4501 ggctccgtat atggattggg tagagcagga agtgaaagcg ctcggcgtga cgcgtttctt
     4561 taaagagaaa ttcttcaccc cagtagcaga agcggcgacc agcggtctga aattcaccaa
     4621 actgcaaccg gcacgagaat tttacgcccc ggttggcacc acgctactgg aggcgctgga
     4681 aagcaataac gttccggttg tcgctgcctg ccgtgcgggt gtttgcggct gctgtaagac
     4741 aaaagtggtt tccggtgaat atacggtgag cagtacaatg acgctgaccg acgccgaaat
     4801 cgctgaaggt tacgtactgg cctgctcctg ccatccgcag ggggatttgg ttctcgcata
     4861 atcgccttat gcccgatgat attcctttca tcgggctatt taaccgttag tgcctccttt
     4921 ctctcccatc ccttccccct ccgtcagatg aactaaactt gttaccgtta tcacattcag
     4981 gagatggaga accatgaaac aaacggttgc agcttatatc gccaaaacac tcgaatcggc
     5041 aggcgtgaaa cgcatctggg gagtcacggg cgactctctg aacggtctta gcgacagtct
     5101 taatcgcatg ggcaccatcg agtggatgtc cacccgccat gaagaagtgg cggcgtttgc
     5161 cgctggcgct gaagcacaac tttcaggaga actggcggtc tgtgccggat cgtgcggccc
     5221 aggcaacctg cacttgatca acggcctatt cgattgccac cgcaataacg ttccggtact
     5281 ggcgattgcc gctcatattc cctccagcga aattggcagc ggctatttcc aggaaaccca
     5341 cccacaagag ctattccgcg aatgtagtca ctattgcgag ctggtttcca gcccggagca
     5401 gatcccacaa gtgctggcaa ttgctatgcg caaagcggtg cttaaccgtg gcgtttccgt
     5461 tgttgtatta ccgggcgacg tggcgttaaa acctgcgcca gaaggggcaa ccacgcactg
     5521 gtatcatgcg ccacagccag tcgtgatgcc ggaagaagaa gagttacgca aactggcgca
     5581 actgctgcgt tattccagca atatcgccct gatgtgtggc agcggctgcg cgggggcgca
     5641 taaagagtta gttgagtttg ccgggaaaat taaagcacct attgttcatg ccctacgcgg
     5701 taaagagcat gtcgaatacg ataatccgta tgatgttgga atgaccgggt taatcggatt
     5761 ctcgtcaggt ttccatacca tgatgaacgc cgacacgtta gtgctactcg gtactcagtt
     5821 cccctaccgt gcgttctacc cgaccgatgc caaaattatt cagattgata ttaatccagc
     5881 cagcatcggc gcgcatagca aggtagatat ggcgctggtc ggcgatatca aatcaaccct
     5941 gcgcgcattg ctgccactgg tggaagaaaa aaccgatcgc aagtttctgg ataaagcgct
     6001 ggaagattac cgcgatgccc gcaaagggct ggacgattta gctaaaccga gcgagaaagc
     6061 cattcacccg caatatctgg cgcagcaaat tagtcatttt gccgccgatg acgccatctt
     6121 tacctgtgac gtcggcacgc caacggtatg ggcggcacgt tatctgaaaa tgaacggcaa
     6181 gcgtcgcctg ttaggttcgt ttaaccacgg ttcgatggct aacgccatgc cgcaggcgtt
     6241 gggtgcgcag gcaaccgagc cggaacgtca ggtggtcgcc atgtgcggcg atggcggttt
     6301 tagcatgttg atgggcgatt tcctctcagt agtgcagatg aaactgccgg tgaaaattat
     6361 cgtctttaat aacagcgtgc tgggctttgt ggcgatggag atgaaggccg gaggttacct
     6421 gacagacggt actgagctgc acgacaccaa ctttgcccgc attgccgaag cgtgcggcat
     6481 tacgggtatc cgtgtagaaa aagcctctga agttgatgaa gccctgcaac gcgccttctc
     6541 tatcgacggt ccggtgttgg tggatgtggt ggtcgccaaa gaagaattag ccattccacc
     6601 gcagatcaaa ctcgaacagg ccaaaggttt tagcctgtat atgttgcgcg caattatcag
     6661 cggacgcggc gatgaagtga tcgaactggc gaaaacgaac tggctaaggt aaaaagggtg
     6721 gcatttcccg tcataataag gacatgccat gattgattta cgcagtgata ccgttacccg
     6781 accgagccgc gccatgctcg aagcgatgat ggccgccccg gttggggacg acgtttacgg
     6841 agacgaccct accgttaatg ctctgcagga ctatgcagca gagctttccg gtaaagaagc
     6901 cgccattttt ctgcctaccg gcactcaggc caacctggtc gctctgctca gtcactgcga
     6961 acgtggcgaa gagtatattg tcggtcaggc cgcgcataac tatctgtttg aagccggtgg
     7021 cgcagcggtg ctgggcagta ttcagccgca acccatcgac gcggctgccg acggcacgct
     7081 accgctggat aaagtggcga tgaaaatcaa acccgacgat atccatttcg cccgcaccaa
     7141 attactcagt ctggaaaaca cccacaacgg caaagtgctg ccgcgtgaat acctgaaaga
     7201 agcatgggaa tttacccgcg agcgcaatct ggcgctgcat gttgacggtg cgcgcatctt
     7261 taatgccgtg gtggcttacg gctgcgaact gaaagagatc acgcaatatt gtgattcgtt
     7321 caccatttgc ctgtcgaaag gtcttgggac gccagtcggt tcattactcg tcggtaatcg
     7381 tgattacatt aaacgtgcca tccgctggcg gaaaatggcc ggtggcggga tgcgtcagtc
     7441 cgggattctg gctgccgccg ggatgtatgc cctgaaaaat aacgtcgcac ggttgcagga
     7501 agatcacgac aacgccgcct ggatggcgga gcaactgcgt gaagcaggcg cggatgtgat
     7561 gcgtcaggac actaatatgc tgtttgttcg cgtcggggaa gaaaatgctg ccgcgttagg
     7621 cgaatacatg aaagcgagaa acgtgctgat taacgcctcg ccgattgtcc gcctggtgac
     7681 gcatcttgac gtctcgcgcg aacaactggc ggaagtcgcc gcccactggc gcgcattcct
     7741 ggcgcgttaa ggagagagac gtgccgcaac gcattttagt tctcggtgcc agtggctaca
     7801 ttggtcagca tctggtgcgc acactcagcc agcaagggca tcagatccta gcggcggcac
     7861 gtcatgtcga caggcttgca aagctgcaac tggcaaacgt cagttgccat aaagtcgatc
     7921 tcagctggcc ggataacctt ccggccctgt tgcaggatat cgataccgtc tattttctgg
     7981 tgcacagcat gggcgaaggc ggcgatttta tcgctcagga gcgccagctg gctctcaacg
     8041 tccgcgatgc gctacgtgaa gtaccagtta agcaattaat ctttctcagt tcgttgcagg
     8101 ccccgccaca tgagcagtcg gatcatctgc gagctcgtca ggctacggcg gacattcttc
     8161 gtgaagcgaa tgtaccagtg accgaacttc gggccggaat tatcgttggc gcaggttcag
     8221 cggcgttcga agtcatgcgc gatatggtct acaacctgcc agtgttaacg ccgccacgct
     8281 gggtacgttc acgcaccacg cccatcgcgc tggaaaactt gctgcactat ctggtggcgc
     8341 tgttagacca tccagccaac gaacaccgca tcttcgaagc cgccggacca gaggtgctca
     8401 gttatcagca acagtttgaa cattttatgg cggtgagcgg taagcgccgc tggttgatcc
     8461 ccat
//
LOCUS       DAECNO010000141         8415 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.141, whole genome shotgun sequence.
ACCESSION   DAECNO010000141 DAECNO010000000
VERSION     DAECNO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.141"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="KKO08_003633"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="KKO08_003633"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBD6319723.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="KKO08_003634"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="KKO08_003634"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBD6319724.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="KKO08_003635"
     CDS             3115..3399
                     /locus_tag="KKO08_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBD6319725.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KKO08_003636"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="KKO08_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBD6319726.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KKO08_003637"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="KKO08_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBD6319727.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KKO08_003638"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="KKO08_003638"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBD6319728.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2020 a         2253 c         2139 g         2003 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gctta
//
LOCUS       DAECNO010000142         8396 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.142, whole genome shotgun sequence.
ACCESSION   DAECNO010000142 DAECNO010000000
VERSION     DAECNO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.142"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            4..817
                     /gene="ttdA"
                     /locus_tag="KKO08_003639"
                     /pseudo
     CDS             4..817
                     /gene="ttdA"
                     /locus_tag="KKO08_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            814..1419
                     /gene="ttdB"
                     /locus_tag="KKO08_003640"
     CDS             814..1419
                     /gene="ttdB"
                     /locus_tag="KKO08_003640"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBD6319729.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1467..2930
                     /gene="ttdT"
                     /locus_tag="KKO08_003641"
     CDS             1467..2930
                     /gene="ttdT"
                     /locus_tag="KKO08_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBD6319730.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="KKO08_003642"
     CDS             complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="KKO08_003642"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBD6319731.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4224..4439
                     /gene="rpsU"
                     /locus_tag="KKO08_003643"
     CDS             4224..4439
                     /gene="rpsU"
                     /locus_tag="KKO08_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBD6319732.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4550..6295
                     /gene="dnaG"
                     /locus_tag="KKO08_003644"
     CDS             4550..6295
                     /gene="dnaG"
                     /locus_tag="KKO08_003644"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBD6319733.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6490..8331
                     /gene="rpoD"
                     /locus_tag="KKO08_003645"
     CDS             6490..8331
                     /gene="rpoD"
                     /locus_tag="KKO08_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBD6319734.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2254 c         2253 g         1862 t
ORIGIN      
        1 gcagtgaata agttgacaga gattgtcgct aactttaccg ccatgatttc tacccgaatg
       61 cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc gtcgatgggg
      121 aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct gaatcgtcct
      181 gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg cttcccactg
      241 cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt caaagcgcca
      301 ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa taccggtagc
      361 ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga aatcgaagtt
      421 tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat gccgtcagaa
      481 ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc cgtaaacgcc
      541 tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc cgccgtactc
      601 tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa agcggcagaa
      661 ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca agggctgacc
      721 ggcaacagtt cagtgatggg cgcatcgtcg cggcacgctg ctggttcatg ccgatctcac
      781 ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa agatcctgac aaccccgatc
      841 aaagctgaag atctgcaaga tattcgcgtc ggcgatgtga tctacctgac cggtacgctg
      901 gtgacctgcc gcgacgtttg tcaccgccgt ttgatcgaac tgaaacgtcc gatcccttac
      961 gatctcaacg gcaaagcgat tttccacgct ggccccatcg tgcgcaaaaa cggtgacaaa
     1021 tgggagatgg tctccgtcgg cccgacaacc agtatgcgta tggaaagttt tgaacgtgaa
     1081 tttattgagc agaccggcgt aaaactggtg gttggcaaag gtggtatggg gccgctgacc
     1141 gaagaaggct gccagaaatt caaggcgtta catgtgattt tcccagcagg ctgcgcggtg
     1201 ctggcggcaa cccaggtaga agagattgaa gaagtgcact ggacagaact cgggatgccg
     1261 gagtcactgt gggtctgccg ggtcaaagag ttcggcccgc tgattgtctc tattgatacc
     1321 cacggcaaca acctgatagc cgaaaacaaa aagctgttcg ccgaacgccg cgatcccatc
     1381 gtggaagaga tctgcgagca cgtccattac atcaaataac cctcccggag aggctcaccc
     1441 ctctcctttt tcgcaggcat aacacgatga aaccttccac tgaatggtgg cgatatcttg
     1501 cgccgctggc ggtcatcgcc attattgctc tacttccggt tcccgcaggg ctggagaacc
     1561 atacctggct ctactttgcc gtttttactg gcgtgatcgt tggactgatc ctcgaacccg
     1621 tgccgggtgc cgtggtggcg atggtgggta tctccatcat cgccatactc tctccctggc
     1681 tgctgttcag ccccgaacag ctcgcccagc caggctttaa gttcaccgcc aaatccctct
     1741 cctgggcagt ttctggtttt tctaattcgg ttatctggct cattttcgcc gcctttatgt
     1801 ttggcacagg ctatgaaaaa accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa
     1861 agatggggca tcgcacgctg tttctcggct atgcggtgat gttctccgag ctgatcctgg
     1921 cacctgtaac accgtccaac tcggcgcgtg gtgcgggtat tatctatccc atcatccgta
     1981 acctgccacc gctctatcaa tcacaaccaa acgacagcag ttcgcgcagc attggctcgt
     2041 acatcatgtg gatggggatt gttgccgact gcgtgaccag cgccattttc ctgacggcga
     2101 tggcacctaa cttgctgtta attggactga tgaaaagcgc atctcacgcc acgctgagtt
     2161 ggggcgactg gttcctcggg atgttgccgc tcagcatttt actggttctg ctggttccct
     2221 ggctggctta cgtgctgtac ccgccggtac tgaagtctgg tgatcaggtg ccgcgctggg
     2281 cagagacgga actgcaggca atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc
     2341 tgatggtagg cgcgctggtg ctgtggattt tcggcggtga ttatatcgat gccgcgatgg
     2401 tcggttacag cgtggtggcg ctgatgctgc ttctgcgcat tatcagttgg gacgacattg
     2461 tcagtaataa agcggcatgg aacgttttct tctggctggc ctcgcttatc accctcgcga
     2521 ccggactcaa caataccggt tttattagct ggtttggcaa actgttagca ggcagcttaa
     2581 gtggttattc gccgacgatg gtgatggtga cgttgattgt ggtgttttat ctactgcgct
     2641 actttttcgc cagcgccacg gcgtatacct gcgccctcgc accgatgatg atcgccgccg
     2701 cgctggcgat gccggaaatc ccgctgccgg tgttctgcct gatggttggc gcggcaattg
     2761 gtctggggag cattcttaca ccatacgcca ccggacccag tccgatttac tacggtagtg
     2821 gttatctgcc aacggcggat tactggcgac tgggggcgat ttttgggctg atattcctcg
     2881 tgttgctggt gattaccggc ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg
     2941 tgccggaaat tgctctccgg cacccaatag ttttacgcag ccggtaactc cgccagcggc
     3001 cagcgcggac gcacgctaac accgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt
     3061 ccggcatagg cgatcatcgc gccgttatca gtacaaaatt ccggacgcgc gtagaacact
     3121 tcgccgcggc gttttttcat catttcagcc agcttcgccc gtaacgtgcg gttagcactc
     3181 acgccgcccg ccatgaccag tcgcttaaag cccgtctgat ccaacgctcg cttacactta
     3241 atcatcaacg tatcgaccac cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg
     3301 tcgtcggtgc cgttgtcacg aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg
     3361 aaatccagcc ccggacggtc ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc
     3421 tgagccgcca ttttcgacag taacggtccg ccaggataat ccagccccag cagcttcgcg
     3481 gttttatcaa acgcttcacc ggcggcatca tcgatagact cgccgagcag ctcgtactga
     3541 ccaatgccag tcacgctgat taactgcgta tgaccgccgg acaccagcag cgcgacaaac
     3601 ggaaattccg gcgggttatc ttccagcatc ggcgctaaca gatgcccttc catatggtgt
     3661 accgggattg ccggaacgtt ccaggcaaac gccagagaac gccccacggt tgcgccaacc
     3721 agcagtgcgc cgactaatcc agggcctgcg gtataggcca cagcatcaat gtcttttgcc
     3781 gttaaaccag actcctttag cgccgcctgg atcaacggta cggttttacg cacatgatcg
     3841 cgggaggcca gttcaggcac gacgccgccg tagtcagcgt gcaatttcac ctgactatac
     3901 aattggttgg ctaacaaacc tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag
     3961 gaagtttcaa tacccagtac acgcatgact tgttttacct cgctttatta ccgcgcagtg
     4021 taggaccaat gcgggttgat gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac
     4081 tcctcaccct tataaaagtc cctttcaaaa aaggccgcgg tgctttacaa agcagcagca
     4141 attgcagtaa aattccgcac cattttgaaa taagctggcg ttgatgccag cggcaaaccg
     4201 aattaatcaa aggtgagagg cacatgccgg taattaaagt acgtgaaaac gagccgttcg
     4261 acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc aggtgttctg gcggaagttc
     4321 gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa gcgcgctaaa gcttctgcag
     4381 tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg ccgcactcgt ctgtactaat
     4441 tccccgagag cgttgctctc cgatcagacc gagttgtagt tgtaaggccg tgcttccgaa
     4501 aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa tcggggccta tggctggacg
     4561 aatcccacgc gtattcatta atgatctgct ggcacgcact gacatcgtcg atctgatcga
     4621 tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac gcgtgttgtc cattccacaa
     4681 cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag ttttaccact gctttggatg
     4741 tggcgcgcac ggcaacgcga tcgacttcct gatgaactac gacaagctcg agttcgtcga
     4801 aacggtcgaa gagctggcag caatgcacaa tcttgaagtg ccatttgaag caggcagcgg
     4861 ccccagccag atcgagcgcc atcagaggca aacgctttat cagttgatgg acggtctgaa
     4921 tacgttttac caacaatctt tacaacaacc tgttgccacg tctgcgcgcc agtatctgga
     4981 aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg attggttttg cgccccccgg
     5041 ctgggacaac gtcctgaagc ggtttggcgg caatccagaa aatcgccagt cattgattga
     5101 tgcggggatg ttggtcacta acgatcaggg acgcagttac gatcgtttcc gcgaacgggt
     5161 gatgttcccc attcgcgata aacgcggtcg ggtgattggt tttggcgggc gcgtgctggg
     5221 caacgatacc cccaaatacc tgaactcgcc ggaaacagac attttccata aaggccgcca
     5281 gctttacggt ctttatgaag cgcagcagga taacgctgaa cccaatcgtc tgcttgtggt
     5341 cgaaggctat atggacgtgg tggcgctggc gcaatacggc attaattacg ccgttgcgtc
     5401 gttaggtacg tcaaccaccg ccgatcacat acaactgttg ttccgcgcga ccaacaatgt
     5461 catttgctgt tatgacggcg accgtgcagg ccgcgatgcc gcatggcgag cgctggaaac
     5521 ggcgctgcct tacatgacag acggccgtca gctacgcttt atgtttttgc cggatggcga
     5581 agaccctgac acgctggtac gaaaagaagg taaagaagcg tttgaagcgc ggatggagca
     5641 ggcgatgcca ctctccgcat ttctgtttaa cagtctgatg ccgcaagttg atctgagtac
     5701 ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca ttgatatcgc aagtgccggg
     5761 cgaaacgctg cgaatatatc ttcgtcagga attaggcaac aaattaggca tacttgatga
     5821 cagccagctt gaacgattaa tgccaaaagc ggcagagagc ggcgtttctc gccctgttcc
     5881 gcagctaaaa cgcacgacca tgcgtatact tatagggttg ctggtgcaaa atccagaatt
     5941 agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat aagctccctg gacttggctt
     6001 attcagagaa ctggtcaaca cttgtctctc ccagccaggt ctgaccaccg ggcaactttt
     6061 agagcactat cgtggtacaa ataatgctgc cacccttgaa aaactgtcga tgtgggacga
     6121 tatagcagat aagaatattg ctgagcaaac cttcaccgac tcactcaacc atatgtttga
     6181 ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt gagcgcacgc atggtttaag
     6241 caacgaagaa cgcctggagc tctggacatt aaaccaggag ctggcgaaaa agtgatttaa
     6301 cggcttaagt gccgaagagc gatcgggaag cccccgacag ccgcactgag aggcagcggc
     6361 aaatatataa gtacgccctc gtaattatcg ttggcggtaa acaaccgttg gatttcagcg
     6421 ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa cctcatgaaa taagtgtgga
     6481 taccgtctta tggagcaaaa cccgcagtca cagctgaaac ttcttgtcac ccgtggtaag
     6541 gagcaaggct atctgaccta tgccgaggtc aatgaccatc tgccggaaga tatcgtcgat
     6601 tcagatcaga tcgaagacat catccaaatg atcaacgaca tgggcattca ggtgatggaa
     6661 gaagcaccgg atgccgatga tctgatgctg gctgaaaaca ccgcggacga agatgctgcc
     6721 gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa tcgggcgcac gactgacccg
     6781 gtacgcatgt acatgcgtga aatgggcacc gttgaactgt tgacccgcga aggcgaaatt
     6841 gacatcgcta agcgtattga agacgggatc aaccaggttc aatgctccgt tgctgaatat
     6901 ccggaagcga tcacctatct gctggaacag tacgatcgtg ttgaagcaga agaagcgcgt
     6961 ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag aagaagatct ggcacctacc
     7021 gccactcacg tcggttctga gctttcccag gaagatctgg acgatgacga agatgaagac
     7081 gaagaagatg gcgatgacga cagcgccgat gatgacaaca gcatcgaccc ggaactggct
     7141 cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa cgcgtgacac catcaaagcg
     7201 aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga aactgtctga agtattcaaa
     7261 cagttccgcc tggtgccgaa gcagtttgac tacctggtca acagcatgcg cgtcatgatg
     7321 gaccgcgttc gtacgcaaga acgtctgatc atgaagctct gcgttgagca gtgcaaaatg
     7381 ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa ccagcgatac ctggttcaac
     7441 gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc acgatgtctc tgaagaagtg
     7501 catcgcgccc tgcaaaaact gcagcagatt gaagaagaaa ccggcctgac catcgagcag
     7561 gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga aagcccgccg tgcgaagaaa
     7621 gagatggttg aagcgaactt acgtctggtt atttctatcg ccaagaaata caccaaccgt
     7681 ggcttgcagt tccttgacct gattcaggaa ggtaacatcg gcctgatgaa agcggttgat
     7741 aaattcgaat accgccgtgg ttacaagttc tccacctacg caacctggtg gatccgtcag
     7801 gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc gtattccggt gcatatgatt
     7861 gagactatca acaaactcaa ccgtatttct cgccagatgc tgcaagagat gggccgtgaa
     7921 ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg aagacaagat ccgcaaagtg
     7981 ctgaagatcg ccaaagagcc aatctccatg gaaacgccga tcggtgatga tgaagattcg
     8041 catctggggg atttcatcga ggataccacc ctcgagctgc cgctggattc tgcgaccacc
     8101 gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc tgaccgcgcg tgaagcaaaa
     8161 gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc acacgctgga agaagtgggt
     8221 aaacagttcg acgttacccg cgaacgtatc cgtcagatcg aagcgaaggc gctgcgcaaa
     8281 ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc tggacgatta atcggtaggc
     8341 cggatcaggc gttacgccgc acccggcact agtctctctg cacaaacgcc accttt
//
LOCUS       DAECNO010000143         8356 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.143, whole genome shotgun sequence.
ACCESSION   DAECNO010000143 DAECNO010000000
VERSION     DAECNO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.143"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            66..314
                     /locus_tag="KKO08_003646"
     CDS             66..314
                     /locus_tag="KKO08_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBD6319735.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            379..747
                     /locus_tag="KKO08_003647"
     CDS             379..747
                     /locus_tag="KKO08_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBD6319736.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            841..1494
                     /gene="nfsB"
                     /locus_tag="KKO08_003648"
     CDS             841..1494
                     /gene="nfsB"
                     /locus_tag="KKO08_003648"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBD6319737.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1602..2849
                     /gene="ybdG"
                     /locus_tag="KKO08_003649"
     CDS             1602..2849
                     /gene="ybdG"
                     /locus_tag="KKO08_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBD6319738.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2917..4293)
                     /gene="pheP"
                     /locus_tag="KKO08_003650"
     CDS             complement(2917..4293)
                     /gene="pheP"
                     /locus_tag="KKO08_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBD6319739.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4394..7537)
                     /gene="cusA"
                     /locus_tag="KKO08_003651"
     CDS             complement(4394..7537)
                     /gene="cusA"
                     /locus_tag="KKO08_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBD6319740.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7549..8067)
                     /gene="cusB"
                     /locus_tag="KKO08_003652"
                     /pseudo
     CDS             complement(7549..8067)
                     /gene="cusB"
                     /locus_tag="KKO08_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2042 a         2326 c         2031 g         1957 t
ORIGIN      
        1 gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
       61 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
      121 tcctctcttg cctgctgctg cccgcccccg cactggggct gacgctggca caaaaactgg
      181 tgaccatgtt ccatctgatg gatcttagcc agctttacac tttattgttt tgtctgtggt
      241 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
      301 cgctggcgga ttaaacgtcg attatcgccg aacttcgcgg catactttgc ttattctcct
      361 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctagct
      421 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
      481 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
      541 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
      601 tatcacatga ataaaaagca ctggatttcg gtgtattccg gcgaggaaat ctccgaagcg
      661 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
      721 gatcaaaaaa gagtgcgtcc aggctaaagc gggaatctat agcgcatttt tctcgcttac
      781 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tacttacaca tggagtcttt
      841 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
      901 aaacttaccc cggaacaggc cgagcagatc aaaacgctac tgcaatacag cccatccagc
      961 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     1021 gccaaatccg ctgccggtaa ttatgtgttc aacaaacgta aaatacttga tgcctcgcac
     1081 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     1141 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     1201 cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     1261 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     1321 gcggtgccca tcgaaggttt tgacgccgca atcctcgatg cagaatttgg tctgaaagag
     1381 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     1441 gctacgctgc cgaaatctcg tctgccgcaa aacaccacct taaccgaagt gtaattctct
     1501 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     1561 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     1621 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     1681 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     1741 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     1801 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     1861 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     1921 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     1981 agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     2041 tcggcgcaat cctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     2101 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     2161 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     2221 ggctggaaat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     2281 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     2341 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     2401 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     2461 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     2521 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     2581 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgacct
     2641 taatggtacg ccaactggct ccaggtgata acggtttacc gctcgagatc tacgcgttta
     2701 ctaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     2761 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     2821 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     2881 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa
     2941 tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc
     3001 catcgtgcac atcagcagca aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa
     3061 cggatagagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ccgcgcgaaa
     3121 acgcagatgc gccaggcaga tcataatcca gttcaacagc aacgttgcta ccaccagcgc
     3181 catcagcaga ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt
     3241 gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa
     3301 aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac
     3361 cccgctgtta tacaccgaca gcgatgccac tagaatgacg aagttcagcg cagaagctac
     3421 cacattgctg tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc
     3481 cacccacgga tagagtgcca gtaaaaccac cagtgaaccg atataaaaca gcaggatgcg
     3541 atacaccacc tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc
     3601 tgtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat
     3661 cagcccattc cagccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc
     3721 tttctcgccg ccgtaaccag aaaacagcag ccacaggcca aagccgatca taccgatgat
     3781 cgccagcact ttaatcagcg caaaccagaa ctcggtttcg ccatataagc gcacgttcac
     3841 caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc
     3901 cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac
     3961 gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata
     4021 ggcaaagtgg gcaaatgaac cggataccgg ctcttcgacc accatctcgc caagctggcg
     4081 cataatcagg aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc
     4141 catctgaatc gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccagcgc
     4201 aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc
     4261 agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt
     4321 gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac
     4381 gaggtatcct gctttaattc cgtacccgat gtcggtgcag ccacatcagc ttatacgccg
     4441 ccgggataat aaacagcgac agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg
     4501 caatccggct catcacctct gaaccagccc ccgttcccca cagaatcggc agcagaccgg
     4561 cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg caggaccgcg ccgtgataca
     4621 acgcctcatc cagcttctgc tcgctgaatg tttgtggatt attcaacgac ggctcggcct
     4681 ctatggcgtg acgtaaatac atcagcatca ccacgccaaa ttcggcggcg accccggcaa
     4741 gggcaataaa gccggtaccc gtcgccacag aaagatgaaa gcccatccac cacagtaacc
     4801 agatgccgcc caccagcgca aacggtacgc tgctgataat cagcaacgct tcgcccaccc
     4861 gacggaatgc cagatacaac agcacgaaga tgatcatcag tgtcatcggc accatgagtt
     4921 tcagcttatg gttggcgcgc tccagtagct cgaactgccc ggagaatgcc acgctggtgc
     4981 ccggttttaa ctgcactttt tcagctatcg ctttttgcaa atcgtgaacc accgacacca
     5041 tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg gcgcgcattc tcggttttca
     5101 gcatcgacgg tccggtagag actttaacgt cggccacgtc tgccagggtg atttgctgct
     5161 tcatcggcgt caggatcggc aactggcgca gtgcctgcgg actatcgcgc cagctttgcg
     5221 gataacgcag attaattgga taacgggcaa tcccttccac cgtttcgcca accatcgccc
     5281 cgcccaccgc agaagtcaca aacaactgca catccgccac cgtcataccg taacgtgcgg
     5341 ctttttcacg gttaatctca acgttgatat agcgcccacc ttccagccgc tcagcaagcg
     5401 cagaagctac gcctggcacc gttcgcgcca cttcttcaat ttgctcagcc atcgcgtcga
     5461 tatccgccag cacagtgccg gaaactttaa tgccgatggg gcttttaatg ccggttgaga
     5521 gcatatcgat acggttacga attggcggca cccacagatt cgccagcccc ggcagacgca
     5581 cggtgttatc cagttcctca atgattttgt ccattgtcat gcctggccgc cactgatcct
     5641 gtggcttaag ctggatggtc gtttctacca tctccagcgg agcagaatcg gtggcggttt
     5701 ccgctttccc ggttttgcca aatacccgcg ccacttcagg tacgctcata attagcttgt
     5761 cggttttttg cagcatactc gccgcctctg ctgcggaaat ccccggcagc gtcgatggca
     5821 tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc cccgccaact ttattgagcg
     5881 gccagagaac cgtcagcacc gaaagcgccg ccaccagcag cgtggttttc ggccagtgca
     5941 gtactttcag caacagcgga tgataaacac gaatcaaaaa gcgattgagc ggattactgc
     6001 tttccggcgg aattttgcca cggatccagt aacccatcag gatagggatc actacgatcg
     6061 ccagcagcgc cgcacccgcc atcgcatacg ttttggtgaa cgccaacggg ccaaacagac
     6121 gcccttcctg cccttccagg gtgaagatcg ggataaacga caacgtgata atcagcagac
     6181 tgataaacag cgccggcccc acttcaacag acgcatcggt gatcacctgc cagcgcgttt
     6241 tattatccag cgtggcgtca ggatgctggt gctgccactc ttccagccgt ttatgcgcat
     6301 tctcgatcat gacgatagcg gcatcgacca tcgccccgac ggcaatcgca atgccaccca
     6361 gcgacataat attggcattc agcccctgga agggcatgac aataaaagca atacacaacc
     6421 ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac atgccagaga aacagcgcgc
     6481 agactaccgc caccacaata aactcttcca gcaacttgcc gctgaggttg tcgatggccc
     6541 ggtcaatgag ctggctgcgg tcgtatgttg taactatctc cacgccttcc ggcagactac
     6601 ttttcagcgt ttccagtttg tccttcacgg cggcgatcac ttctcgggcg tttttgccgg
     6661 atcgcaggat caccaccccg cccgccactt cgccttcgcc gtttagttcg gcaatgcccc
     6721 ggcgcatctt cgggccaatc tggaccttcg caacatcgcg cagataaacg ggcacgccat
     6781 tttcactggc ttttaaaacg atgtgattaa agtcgtcgag cgtttgtaga tagccgctgg
     6841 cgcgcaccat atattctgct tccgccagtt cgattgacga accgcccgct tcctggtttg
     6901 aagcatccag cgcgcttttt acttcggcga gactgatgcc atactgcgcc aggcgctgag
     6961 gatcgataac cacctgatac tctttcacca caccgcctac cgacgccact tccgcaacgt
     7021 caggaatggt tttcagctca tatttgagaa accagtcctg caatgagcgt aaatcggcaa
     7081 gatcgtgctt accgctgcga tccaccagtg catattcata gatccagcca acacccgtgg
     7141 catctggtcc cagctcggcg ctgactcccg caggcagctt accctgtacc tggttgaggt
     7201 actccagcac ccgcgaacgc gcccagtacg gatcggtgcc atcttcgaaa atgacataca
     7261 cataagagtc gccaaactgc gagaaaccac gcacagtctt cgcgccaggc accgacaaca
     7321 tggtggtggt tagcggataa gtcacctgat tttcaacgat ttgcggtgcc tggccgggat
     7381 agctggtttt aataatcacc tgcacatcgg agagatccgg caacgcatcc actggcgtat
     7441 taatgatggt ccaggtgccc cagatgctca gaaacaacgc gcccatcagc accaaaaaac
     7501 ggttcgccac cgagcgacga ataatccatt caatcattgg ttattccctc aatgcgcatg
     7561 ggtagcactt tcagagcgca tccgctccag tgcgccagaa atattggctt cggaatcaat
     7621 caggaacagg ccgccggaaa ccaccttttc accttccgcc agaccagagc gtaatgcggt
     7681 gacgccttgc gatgcctgga aaacagcaac gcgtttcggt acaaagcgcc cgtcggcatc
     7741 aacggtaatc acccgctgtt cgttgccggt atcaatcagc gcctgtgacg gaatgagcag
     7801 catcggttcg ctggcggtgt tgagttgcaa ccaggcgttc atgcccggtt ttagcgcctc
     7861 gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg
     7921 tagcagcgtc catttgcgga tggtgagtgt tttatccggt cgcgccggaa cggtgagggt
     7981 aaactgcgag gcatctttca ccagccaggc gatggactcc gggatcgcag cagtgaccca
     8041 caccgggtcc ataccctgaa ttcgcgatta cgcgcaatcc ccctgagaac cgccatgaac
     8101 aagggtttca tgatttgtga ttttagtttg gtacgcaatt tggtacacaa cactattttc
     8161 acttcagcgg gtagtcatca aactcaccat aacgagcatc gttgatgatg tatgtgatca
     8221 ccacaaatat cacatctgga ctagttccgt cttcatgcgc aggtagactg gccccctgaa
     8281 tctccagaca accagtatca cttatttaag tgatagtctt aatactagtt tttagacttg
     8341 tcattggaga acagat
//
LOCUS       DAECNO010000144         8210 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.144, whole genome shotgun sequence.
ACCESSION   DAECNO010000144 DAECNO010000000
VERSION     DAECNO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.144"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..306
                     /locus_tag="KKO08_003653"
     CDS             <1..306
                     /locus_tag="KKO08_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBD6319741.1"
                     /translation="LLIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMAL
                     SSTLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            278..2206
                     /locus_tag="KKO08_003654"
     CDS             278..2206
                     /locus_tag="KKO08_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBD6319742.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2190..2393
                     /locus_tag="KKO08_003655"
     CDS             2190..2393
                     /locus_tag="KKO08_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6319743.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2390..3983
                     /locus_tag="KKO08_003656"
                     /pseudo
     CDS             2390..3983
                     /locus_tag="KKO08_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3973..5478
                     /locus_tag="KKO08_003657"
     CDS             3973..5478
                     /locus_tag="KKO08_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBD6319744.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5515..5862
                     /locus_tag="KKO08_003658"
     CDS             5515..5862
                     /locus_tag="KKO08_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBD6319745.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5920..6948
                     /locus_tag="KKO08_003659"
     CDS             5920..6948
                     /locus_tag="KKO08_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBD6319746.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7000..7386
                     /locus_tag="KKO08_003660"
     CDS             7000..7386
                     /locus_tag="KKO08_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD6319747.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7398..7775
                     /locus_tag="KKO08_003661"
     CDS             7398..7775
                     /locus_tag="KKO08_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6319748.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7762..>8210
                     /locus_tag="KKO08_003662"
     CDS             7762..>8210
                     /locus_tag="KKO08_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6319749.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPI"
BASE COUNT         1898 a         1933 c         2539 g         1840 t
ORIGIN      
        1 ctactcattg ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat
       61 caggtgtcaa aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg
      121 gcgatggctt tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg
      181 gatttaacgc cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag
      241 tgcgcgcgtg cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact
      301 gagtaatatg atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt
      361 gacggctgta gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga
      421 gggggaatgg aaaacgctgc cgtttcaggt cgccatcatg aactgcatgg gcaatgacga
      481 gattcgtaca gttaacctga ttaaatctgc ccgtgttggt tatacaaaaa tggtgcttgg
      541 tgtgatcggg tattttattg agcacaaatc ccgcaacact cttctttttc agccgacgga
      601 ctcagctgct gaagatttta tgaagtctca cgtggaagca acaattcgtg atgttccttg
      661 cctgaaaaaa ctttctccct ggctgggccg gaaacaccgg gataatacgc tcacactgaa
      721 gcgtttttcc tctggtgtgg ggttctggtg tttgggtggt gctgctgcca aaaactaccg
      781 tgaaaaatct gtcgatgtgg tctgctatga cgaactctcc tcgtttgaac cggatgtgga
      841 aaaagaaggt tcgccaacac tgcttggcga taaacgtatt gaaggttcgg tatggccaaa
      901 atccatacgc ggttcaacgc caaaaattaa aggctcctgt cagattgaaa aagctgcgaa
      961 tgaatctgcg catttcatgc ggttttatgt cccttgccct cattgcgggg aggagcagta
     1021 tctgaagttt ggcgatgatg cgacgtcgtt tggcctgaaa tgggagaagg gtaaaccgga
     1081 aacggtgtat tacctgtgtg aacataatgg ctgcgtgatc cgtcagtctg aacttgatca
     1141 gaccgggggg cgctggattt gtgacaatac cgggatgtgg acgcgtgacg gtctgacgtt
     1201 tttcagtgct tcgggtaatg aaattccgcc gccgcgctcc atcactttcc atatatggac
     1261 ggcgtacagt ccgttcacca cctgggtaca gatagtctgt gactggctgg atgcactgaa
     1321 agatcccaac ggcgtgaaaa cttttgtgaa caccacgctg ggcgagacct gggaagaggc
     1381 cgtgggcgaa aaactcgatc accaggtact gatggataag gttgtgcctt acacggcaac
     1441 ggtgcctgtc cgggtggttt atctgacggc gggcattgac tcgcagcgaa accgttttga
     1501 gatgtatgtc tgggggtggg ctccgggaga ggaagccttt ctggtggata aaatcatcat
     1561 catgggacgt cctgatgagg aagagacgct gttacgtgtg gatgcggcga tcaataaaaa
     1621 ataccgccat gcagacggta ccgaaatgac catttcccgt gtctgctggg atatcggggg
     1681 gatcgatggc gaaatcgtgt atcagaggtc aaaaaaacac ggtgttttcc gggtactgcc
     1741 ggtaaaaggt gcctctgtct atggcaagcc ggtgatcacc atgccgaaaa cccgcaatca
     1801 gcggggtgtg tatctgtgtg aggtggggac ggataccgca aaagaaattc tctatgcccg
     1861 aatgaaggcc gatcccaccc ctgtgaatga ggccacatcg tatgccatcc gctttcctga
     1921 taatccggag attttttcgc agacagaggc acaacaactg gtggcggagg aactggtgga
     1981 aaagtgggaa aaaggaaaga tgcgtctgtt gtgggataac aaaaagcggc gtaacgaagc
     2041 gctggactgc ctggtgtatg cctatgcggc attacgtgtg tccgtgcaac gctggcaact
     2101 tgatctggct gtgctggcgg catcccgcaa ggatgcaact gcacaaccga cccttgaaga
     2161 actggcagcg aagctgtccg gaggagtgaa tggttacagt cgctgaactg caggcgctgc
     2221 gtcaggcgcg ccttgattta ttaaccggta aacgggttgt gtcggtacag aaagacggtc
     2281 gcaggatcga atatacggca gcgtccctga atgagcttaa ccgtgcgatc aacgatgcgg
     2341 agtcggttct gggtacgacc cgacgccgcc gaccgctggg agtgaggtta tgaaacgaac
     2401 gcctgtcctg attgatgtga acggcgttcc gcttcgtgag agtctcagct acaacggagg
     2461 cggcgcagga tttggcgggc aaatggcaga gtggttacca ccggcgcaga gtgccgatgc
     2521 ggccctgttg cctgcattgc gtctggggaa tgcccgggca gatgatctgg tgcgcaataa
     2581 cggtatcgcg gctaatgcgg tggccctgca taaggatcat atcgtcgggc acatgtttct
     2641 tatcagctac cgtctgaact ggcgttggct ggggatgcgg gagaccacgg caaaaagttt
     2701 tgtcggggag gtggaagcag cctggtcgga atacgcagaa ggaatgtttg gcgagatcga
     2761 cgtggaaggg aaacgcacgt ttacggaatt tatccgtgaa ggggtgggcg tgcatgcctt
     2821 taacggcgaa atctttgtgc agccggtctg ggatacggaa accacgcagt tattccgtac
     2881 gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca ggacacggta tggggaacca
     2941 ctttctgcgg gccggggttg aggtcgatcg gtatgggcgg gcgattgcgt accatatctg
     3001 tgaggatgat tttccgttct ctggtgccgg acgctgggag cggatcccgc gtgagcttcc
     3061 caccgggcgt ccggccatgc tgcatatttt tgagccggtg gaggacgggc agacccgtgg
     3121 ggccaatcag ttttacagcg taatggagcg gatgaagatg ctggactccc tgcaggccac
     3181 gcagcttcag tcggccattg tgaaggcgat gtatgcagcc acaatcgaaa gtgaacttga
     3241 taccgaaaag gcatttgaat atatcgcggg ggcatcgcaa ggtcagcagg ataatccgct
     3301 tattaatatt ctggagaagt tcacccgctg gtatgacacg aataacgtga cgctgggcgg
     3361 tgttaaaatt ccgcaccttt tttcctggcg atgcgctgaa cctgcagaca gcgcaggatt
     3421 cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatattgca gccggtcttg
     3481 gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttactca agtgcccgtg
     3541 cctccgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt gcggcccggc
     3601 tggccacgca gatgttttcc tgctggctgg aagaggcgct tcttcggggg attattcgtc
     3661 cgccacgggc gcgttttgat ttttaccagg cgcgttcagc ctggtcacgg gcagagtgga
     3721 ttggctccgg aagaatggcc attgacgggc tcaaggaggt ccaggaatcg gtgatgcgca
     3781 ttgaggccgg actgagcacg tatgagaaag aactggcact gatgggcgaa gattatcagg
     3841 acattttccg ccagcaggtc agggaatccg cagagcgcga aaaagccgga ctctcacgtc
     3901 cggtgtggat agcccaggcg tatcagcagc agatagcgga gagtcgcagg ccggaagagg
     3961 agacaacatc acgtgagacg taatctttca catattatcg ctgcggcatt caatgagccg
     4021 ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg tgagatgggc
     4081 gcagcaagcc tttcggtacc gcaacagcag gtacagcttg acgctcccgg gatgctggct
     4141 gaaacggacg agtacatggc cggaggcaaa cgacctgccc gtgtttaccg ggtggtgaac
     4201 ggtattgctg tactgccggt gaccggcacg ctggtacacc ggctgggtgg catgcggcca
     4261 ttttccggaa tgactggcta tgacggcatt gtcgcctgtc ttcagcaggc aatggcggac
     4321 actcaggtac ggggcgtact gctggacatt gacagtcctg gcgggcaggc caccggcgcg
     4381 tttgactgcg ctgacatgat ttaccgtctc cggcagctga agcctgtctg ggcactgtgc
     4441 aatgacacgg cctgttcagc cgccatgtta ctggcatcgg cctgctcccg acggctggtt
     4501 acccagacat cccgtatcgg ctccattggg gtgatgatgg gccatgtcag ctatgccggt
     4561 catctggcac aggccggtgt ggatatcacg ctgatttacg ccggatcgca caaggtggat
     4621 ggcaatcagt ttgaatccct gccagcaaag gttcgccagg acatgcagca gcgcattgat
     4681 gcggcgcgcc ggatgtttgc tgaaaaagtg gcgatgtata ccggtttatc tgtggatgcg
     4741 gttatgggaa cagaggccgc cgtttttgaa ggtcagtccg gtattgaggc cgaactggcg
     4801 gatgaattaa tcaatgcgtc ggacgccatc agcgtgatgg ctgcgacgct gaacacacac
     4861 gatacaggag gcactatgcc gcaattaact gcaacggagg ctgccgcgca ggagaaccag
     4921 cgagtgatgg ggatcctgac gtgtcaggaa gcgaaaggac gtgaacaact ggccacgatg
     4981 ctggcagggc aacagggtat gagcgttgag caggcccggg cgattctggc cgtggcggca
     5041 ccgcaacaat ctggtgtatc tgcgcagagt gaagccgatc gcattatggc atgtgaagag
     5101 gcgaaaggac gtgaacaact ggcggcaacg ctggcggcga tgccggagat gacggtggaa
     5161 aaagcccgtc cgattctggc tgcttcaccg caggcgaatg ccggaccctc acttcgtgat
     5221 cagattatgg ccctggatga ggcaaaaggg gcagaggcgc aggctgaaaa actggcggcc
     5281 tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg cggcagcctc aggcagggca
     5341 gaaccggttt ctgcatccac aaccgccatg tttgaacatt tcatggcgaa ccattcaccg
     5401 gcagcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga cgtgaaaatg
     5461 ctcatggcca tgccatgaag tcagtgctga ccatcaatat gaggttttta caatatggta
     5521 acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa tgatccggcc
     5581 cataccgcca caggcagcag cgggatttcc tcggcaacac cggctctgac gcccctgatg
     5641 ctggatgagg ataccgggaa actggtggtc tgggacggac agaaggcagg ccatgtggtt
     5701 ggcatactgg tactgccgct tgaaggtaca gagacggtgc tgacgtatta caagtcgggg
     5761 acctttgcga cggaggccat ccactggcct gagagagtgg atacacacaa aaaggcgaat
     5821 gcctttgccg gcagtgccct gagtcacgca gcgctgccgt aacattttat caggtcactg
     5881 cggtagcctg actgatttct gaatgaaagg aactgattta tgggattgtt tacgacccgc
     5941 cagttactcg gttataccga acaaaaagtt aaatttcgtg cgctgtttct ggagctgttt
     6001 ttccgccgta cggtgaattt ccataccgaa gaggtaatgc tggacaaaat taccggaaaa
     6061 acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aggtgttgcg tcaccgtggc
     6121 ggtgaaactc gtgtactgcg cccgggctac gttaagccga aacacgaatt taattaccag
     6181 caggcggtgg agcgtcttcc cggtgaagat ccggcccagc tgaacgaccc ggcttaccgt
     6241 cgcctgcgta tcatcaccga taacctcaaa caggaagagc acgccattgt ccaggtggaa
     6301 gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca ccatggaagg agagcagttt
     6361 gaaactgttg aggtcgattt tggccggtcg gtgggaaata acattacgca ggcaggaggg
     6421 acggagtgga gcgctcagga ccgcgatacc tttgatccga cgcatgatct ggatgcgtac
     6481 tgtgattttg cttccggcac catcaatatt gccattatgg atggtaaggt ctggcgtctg
     6541 ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc gtcgtggctc aaattcacag
     6601 ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct tcaaagggta ttatggtgat
     6661 ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg acggtaccga aaaacgttat
     6721 ctgccggacg gcacactggt actgggaaat acggccgcag agggtattcg ttgctacggt
     6781 gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg cgtcttcccg ttatccgaaa
     6841 cactggctga ccgtgggtga tccggcgcgt gaattcacca tgacgcagtc cgcgccgctg
     6901 atggtgctgc cggatccgga tgagtttgtg gtggtgcagg tgaaataatc cgtgagcggg
     6961 agctaaatgc tcccgttttt cattcaatca ggagcgggta tggctacgaa agaagaaaac
     7021 ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg aaccggatgt ctccggtagt
     7081 gcggcagata ttgcgcagcg agtggcggaa ctggaggaag agcttggaga tgcgggggag
     7141 gtcgcagagc cggatacgta ctcaccatcg aaagatgatc cgaccgcgca taaaggcgag
     7201 tcagttcctg aacagccagg cattgttacc ggagacgaaa cggggcaggt tacggtggtg
     7261 gcactggcga cactgcatac aaaatgtaat ggttcagtga tatttgtttc accgggaacg
     7321 tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg caggtggtct ggcaaaaagg
     7381 cagtaacagg agatgtcgtg gacggtttcg ataatctgtt tgatgctgcg cttgccggtg
     7441 tcaatgaagt cattctccgt gatatgggga tcagcgccgt cattacgtcc ggagaacttg
     7501 aggggacaca cctgacaggg gtttttgatg atcccgaaag tatttctttt gttgccgggg
     7561 ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa aacggcagat atatcgcagt
     7621 tacgccgtca ggatacgtta actatcgggg atgattcttt ctttgtggat cgtattactc
     7681 cggatgacgg gggatgctgt tatatccggt taaggcgggg cagtcctgcc ccacagaata
     7741 atgccagaat gaggcgatat gatgaaggga cttgagaatg ccatccgcaa tctgaacagc
     7801 ctcgataccc gcatggtgcc acagaccagc gcatgggcgg taaaccgtgt ggcggcaaag
     7861 attgtttctg tcgccacacg gcaggttgcg cagaataccg ttgccgggga taaccaggtg
     7921 aaaggtattc ccctgaaaac ggtcagggag cgtgtcaggc tgcttaaagc cagcccctca
     7981 ggaaaaatgt atgcccgcat gcgtgttaac cggggtaacc tgcccgccat taagctgggt
     8041 accgcacagg tcagactggc ccgctcccgg catggcagca actcacgtca tcgtggcagc
     8101 gtactgaaag tggggaaata ccttttccgg gatgcgttta ttcagcaact ggcgaatggt
     8161 cgctggcatg tgatgcggcg tattgacggc aaaaatcgtt accccattga
//
LOCUS       DAECNO010000145         7967 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.145, whole genome shotgun sequence.
ACCESSION   DAECNO010000145 DAECNO010000000
VERSION     DAECNO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.145"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            65..553
                     /locus_tag="KKO08_003663"
     CDS             65..553
                     /locus_tag="KKO08_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBD6319750.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            550..2268
                     /locus_tag="KKO08_003664"
     CDS             550..2268
                     /locus_tag="KKO08_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HBD6319751.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            complement(3127..3484)
                     /locus_tag="KKO08_003665"
                     /pseudo
     CDS             complement(3127..3484)
                     /locus_tag="KKO08_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3676..4653
                     /locus_tag="KKO08_003666"
     CDS             3676..4653
                     /locus_tag="KKO08_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6319752.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(4999..6549)
                     /locus_tag="KKO08_003667"
                     /pseudo
     CDS             complement(4999..6549)
                     /locus_tag="KKO08_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            complement(6604..7308)
                     /locus_tag="KKO08_003668"
     CDS             complement(6604..7308)
                     /locus_tag="KKO08_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBD6319753.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEIRLVSRVFEM"
     gene            7372..7551
                     /locus_tag="KKO08_003669"
                     /pseudo
     CDS             7372..7551
                     /locus_tag="KKO08_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1929 a         2024 c         2126 g         1888 t
ORIGIN      
        1 attcagagta gtgaagattt ccaactggtt aaaatggtgg aaaataatta cagggagtat
       61 ctttatggtt aaaataatat cattcttatc cctaatgctt ttgtcaatta gtgcatatag
      121 caatattggg gatatagtta tagatggttc aatcgctaat gctaacagtg ataaaggtta
      181 tgtgattact gaaaagcagt ttgattcgtt cccacgggat accatcgtaa cgacaacacc
      241 ttggacgcca accggtaaaa aagtaaaatt caagggcgtc gacctaagtt atgttttaaa
      301 actcgctggc gctaatggag caaagctaaa attccatgct ctgaatgatt atgaaatcat
      361 tgtagacatg aatgacgttg aaaaatataa cattttattg gctacagaaa tggatggaaa
      421 aaggcttcaa ataagggatt ttggacctta ttttttggta tatcctttag atgaacatta
      481 tattgagctt aacacgccac actatcttgc acggttcatt tggcagatag acaaaattac
      541 ggtgctcaaa tgaaaaaaaa tttctttatc attttggttg ctctgctttt gttgatagca
      601 ttcactgtgt ctagtgttgt taagtacacc agtcttttat cggagaaaaa tttatacacc
      661 gtcgctggca cccaagaaaa ctacggtttc tcagtggcaa aattcattat acagttgaat
      721 gagctttaca cgctaatcaa tagcgataat actattgacg atgttaggct taaattcgat
      781 atcctttatt ctcgtttgaa tgttatttat gtaaagagtg aggcgactgc acctctatat
      841 aaacagcaag gctatgaaga aacaattgat tcgataaaca aaaagcttga agacatagac
      901 gggctgcttt cgcataatca ccctgattat aaaaaaataa gtacaatcat agctgatata
      961 aaaccgctaa ctaaaaccgt tacaaatctt gctgatatgg ctgaaatagc acaaaggaat
     1021 gacgctctta aagatttcag gagcaaacgt cagcagttat ggactctgct ttttattaca
     1081 ggtgggttga tttccttact tctttttgtc ctatttattt acataagtaa aattaaccga
     1141 ttgttactat cagaacgggc tgcttttgcc agtaaaaatg catttttagg tatggtaggc
     1201 catgaactca gaacatcttt acaggcaata gtgtctatca ttgatgtcgt aaccaataat
     1261 ctgagtggag ggattaaatc tggtcagata gaacgacttg aaactgctgt ttcgaaaatg
     1321 gagcgccagt taaatgattt ggctgaattt gcaaaaattg acaacggatc tgtagagata
     1381 aaaaacacct ataatagctt gcaagcaatt gttactaacg ctgtgcagga ttgcattgct
     1441 atttatgaaa aaaaggatgt cacagttaaa ataaaaaaca ataatgatgc agtgattttt
     1501 acagatgctc tacgccttaa tcaagtaatt gaaaacttaa ccagtaacgc tattaagtac
     1561 actgagcgcg gggaagttaa tgtagattac tttatagaaa aaggtaaggt attaaatatt
     1621 gtcatttctg ataccggaaa gggaatccct aaagacaaac taaagttcat atttaaacca
     1681 tttacacgag ttgtagattc gaaatccact gtacctggat ttgggatggg acttgctatt
     1741 gttgcaggga taatcagact attaaaaggt actattcata tatctagtga agtaaatgta
     1801 ggtacaactg ttacagtgag aatacctatc aagttgggag atagcagcct tgcaacagct
     1861 gaggtatctg cttcagctcc cggagatgtc ttcggcgttc ttagcttgct tgttattgat
     1921 gataatgaaa tggcctgttc gtctctttcc agtctgctta ctaatgctgg atacattgtc
     1981 gaggctacaa cctcgcctga acgagccctg gaaaagcttc tgcgtaaacc ttatgacgtt
     2041 gtcctgagtg atttacaaat gccaggcatg acaggtgatg agctgtatgc aactgttagt
     2101 agcagtcaga atcctaatcg atccacgcct ttcatattca tcagcgcata cagtgatgca
     2161 cactctatcg gacaggctcc tttgttaact aaacctgtca ggttaaaaga tataaacctt
     2221 aaaatacatg aggttatgtc aaatcaagga cgagtcaaaa gtgagtaggc attgtcgtaa
     2281 tatttgcgat tatagctttg gtgttctcga tcgttgcgaa ttttgaaaat ttgtggagtc
     2341 aaatggcgcc gaaagcccgg cgtagccggt tacctggcta taaactctgc caccctaccc
     2401 cctgccctga ttagccttaa cccgcttaca gcaatgaaga agtaaaagta atccctttta
     2461 accccggtga gcgggcttta cggcgtttac gacgtaaagg gcgtgatttc cggggcctta
     2521 gcggcggcag cttgctgctg acgcttaggg ggatgttctt cagttatttc agcgacacca
     2581 gggaacttaa cacggtagtt gcaaagctat acccgctaaa aatgtctcag atgcctctgt
     2641 acgcggtcgg agtggtcccg gcaaggcgtc agccgcagcc ggggcgtatc tccgcgttag
     2701 cgggccgaag ggccgcttac gagcgtgtac ccgacaactg accgcagctc cacccacagc
     2761 gatgaagaag agtagttaca acacccatca ttaaaagcga cactactccc ggccttcggc
     2821 cgggggcggc ggcgcttttc agtggtgggg aggggcaccc ggccggcaaa ccggcgccgg
     2881 ttttatgatg agctttcttt tgaatgctgt aactaatttg gttctccggt cggggccctt
     2941 cgcgaagcga tgggagctct ttctgatgtt gccgaaccgg cgcgaccgtc aggtcgctgc
     3001 aagagcgggc attctccgga gcctgtccac agggtaggcg atgaagaggt tatgtgttta
     3061 caggatttat taaaggatct ttaaaagaga tacagaactg gtgtttaaaa ggaactacgc
     3121 gccctgaaag accgacggca ggctcagaag atgctccagc gtggccatag tacgttcacc
     3181 gaagacgtgt gcaacaaccg tcctccgtat cctgtcatac gcgtaaaaca accagcgctg
     3241 gcgtgattta gcgccgacgt aaccccactg ttcgtccatt tccgcgcaaa caatgacgtc
     3301 actgcccggt tgtatgcgtg agtttaccga ctgcggcctg agaggcggtg taagtgaact
     3361 gtagctgcca tgttttacgg cagtgagagc agagatagcg ctgatgtccg gcagtacttt
     3421 taccgttacg caccacgcct tcagtagctg agcaggaggg acaactgatg gagatggaag
     3481 ccacgggagc acctcaaaac caccatcata cactaaatca gtaagttggc agcatcaccg
     3541 taatcgtaca gctaagaaca agtgcttcaa gtctacttat taaaatgtac ctttgaaaaa
     3601 gtagactttc tgcggtacgc tctgacagta agtttgcccc cgatttttta cgcagcgcgc
     3661 cggacggagc ccgacatgat cctgattccc gatgacgaac cggagctgag tctgcccgca
     3721 gccagtgagg aattcctgcc ggcgctgtca ggtgagaatg ccccggtcag cccggcccgg
     3781 gcctacctgc tttcactcaa ttcccaccgc agccggcaga ccatggcctc gttcctgaac
     3841 atcgtcgccg ttatgctcgg agctgcctcc ctggagtcct gcagctgggg cagcctgcgg
     3901 cgccatcacg tgatggccgt gaccgaactg ctgcgcgaca ccggccgggc caccgccacc
     3961 gtcaacacct atctttcggc actcaagggc gtggcgaagg aagcctggat gctccggctc
     4021 atggatgtgg agagcttcca gcatatccgg gcggtccgta acctgcgcgg cagtcggctg
     4081 cccagcggtc gggcgctgcc ccagggggag atccgcgccc tgtttgccgt ctgtgaagcc
     4141 gatcgcagct gcctcggggc gcgggatgca gcgatgctgg cggtcattct cggctgcggc
     4201 ctgcgacgat ccgaagtagt gagcctggat ttgcgtgacg ttgtcactca ggaccgtgcg
     4261 cttagagtgc tgggtaaggg aaacaaggag cgactggcat atgtcccggc cggtgcctgg
     4321 cagcggctgc agatctggat cgatgagatc cggggcgaga ccccgggccc gctgttcaca
     4381 cgcatccgtc gtttcgggga tgtgactcta aaccggctaa ccgaccaggc ggtgtaccat
     4441 atcctgcagg tgcgccaggg tcaggccggc atcacgaaat gttctccaca cgatctgagg
     4501 cgaaccttcg ccaccgcgat gctggataac ggggaggatt taattaccgt gaaggatgcg
     4561 atgggccacg cgagcgtcac caccacccag cagtatgacc gtcgcgggga gcaacgcctg
     4621 caggacgcgc gggatagact caaccttatt tagggctaag taaacacaaa caagggggct
     4681 gtaccccctc taattttaca ttaacacgat actcgacaga ctttcccagt cgtagtcagc
     4741 aaccatcgcc cgatggaggc tgggataaat gctgacagcc cgacgtgaag caaaaggctc
     4801 tcgccgctgg catggcttaa tgtcgcggtc ccctcaatca ctaacacaat ttcagcgctg
     4861 gatgtcatta ccgtttgatt gttcaccgca gtgaaaatac tgaaatggaa atcctcaaca
     4921 ggaataggat agcgcgcttc tgcggcgtgc tgttccggta acatagggga gcccgcagaa
     4981 ttcggaaaaa atcgtacgct aaggttttcc gggcatccgt aagggccgaa acttcccgtc
     5041 ttccagtctg cggctctgcc gccagacgta atcgccggtt aggttgatgt gctcccagcc
     5101 cagcggcgac aggaattgca gcagctcgcc gtccaccggc ttgccggcct cgaccaaccc
     5161 ctgggtggcg cgttccaggt acaccgtgtt ccacagcacg atagccgccg tcaccaggtt
     5221 gaggccgctg gcccggtagc gctgctgctc gaagctccga tccctgattt ccccaaggcg
     5281 gttgaagaac accgccctgg ccagcgagtt gcgcgcctca cctttgttca ggccggcatg
     5341 cacgcggcgg cgcagttcaa cactttgcag ccagtccagg atgaacagcg tgcgctcgat
     5401 ccggcccagc tcgcgcaggg ccacggccag tccgttctgg cgcgggtagc tgccgagctt
     5461 gcgcagcatc agcgaggcgg tgacggtgcc ctgcttgatc gagctggcca ggcgcaggat
     5521 gtcgtcccag tgggcacgca cgtgcttgat gttcagggtg ccgccgatca gcgggcgcaa
     5581 cgtcgggtag gcttgcacgc cctgcggcac gtacagcttg gtttcgccga ggtcgcggat
     5641 gcgcggcgcg aagcggaagc ctagcaggtg catcagggca aagacgtgat cggtgaagcc
     5701 ggccgtgtcg gtgtagtgct cctcgatccg caggtcggac tcgtggtaca gcaggccgtc
     5761 gagcacatag gtggaatcgc ggacgccgac attcaccacg cgggtgctga acggcgcgta
     5821 ctggtcggag atatgggtat agaacagccg tcccggctcg ctaccgtact tcgggttgac
     5881 gtgcccggtg ctctcgcccc ggccacccgc gcggaagcgc tggccatcgg aggatgaggt
     5941 cgtgccgtcg ccccagtggg cggcaaaggc gtggcgatac tggtggttga ccagctcggc
     6001 caaggccgcc gaataggttt cgtcgcggat gtgccaggct tgcagccagg acagcttggc
     6061 gtaggtcagg ccggggctcg actcggccat cttggtcagc ccgaggttga tcgcatcacc
     6121 gaggattgcg gacagcagca acgtcctgtc tttggcctcg gccccgtcct tcaagtgggt
     6181 gaagtggcgg ctgaagcccg tccagtcgtc cacgtccatc agcagttcgg tgatcttgat
     6241 gcgcggcagt aactggctgg tttggtcgat cagcgcctgc gcccgatccg gcaccgccgc
     6301 atccagcggg gtgattttca gccctgactc ggtgaggatg gcatcgggca gctcgttgtc
     6361 cttggccagg cgggtgacgg tggccaactg ctcgtccagc agctgcaaac gctcttccag
     6421 gtactggtcg ctgttcgggt tgatcgccag gggcagggcc tgctcgcgct tgagtgcggc
     6481 gaacttctcg gccggcagca ggtagtcgtc gaagtcgcgg aactgccgcg agcccttgac
     6541 ccagatgtcg cggcactgtt gcaaagttag cgatgaggca gccttttgtc ttattcaaag
     6601 gccttacatt tcaaaaactc tgcttaccag gcgtatttcg cccaggggat caccataata
     6661 aaatgctgag gcctggcctt tgcgtagtgc acgcatcacc tcaatacctt tgatggtggc
     6721 gtaagccgtc ttcatggatt taaatcccag cgtggcgccg attatccgtt tcagtttgcc
     6781 atgatcgcat tcaatcacgt tgttccggta cttaatctgt cggtgttcaa cgtcagacgg
     6841 gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg ctttatccgt
     6901 gttgatgaat cgcgggatct gccacttctt cacgttgttg aggattttac ccagaaaccg
     6961 gtatgcagct ttgctgttac gacgggagga gagataaaaa tcgacagtgc ggccccggct
     7021 gtcgacggcc cggtacagat acgcccagcg gccattgacc ttcacgtagg tttcatccat
     7081 gtgccacggg caaagatcgg aagggttacg ccagtaccag cgcagccgtt tttccatttc
     7141 aggcgcataa cgctgaaccc agcggtaaat cgtggagtga tcgacattca ctccgcgttc
     7201 agccagcatc tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc
     7261 ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat
     7321 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc cctccgccac
     7381 tcgttcgcga cggccttgct ccgcagcggt tacgacattc gaaccgtgca ggatctgctc
     7441 ggccattccg acgtctctac gacgatgatt tacacgcatg tgctgaaagt tggcggtgcc
     7501 ggagtgcgct caccgcttga tgcgctgccg cccctcacta gtgagaggta gggcagcgca
     7561 agtcaatcct ggcggattca ctacccctgc gcgaaggcca tcggtgccgc atcgaacggc
     7621 cggttgcgga aagtcctccc tgcgtccgct gatggccggc agcagcccgt cgttgcctga
     7681 tggatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     7741 tctgaaaacg acaaacagcc agaaaggctg ttacaggcga tttgatctgc aacctattgg
     7801 ttaaattaat gtatcaaaaa cgatggtttt tgtgacagtc ttgaaaagtc ctgacttctc
     7861 ccgaaaaatg actcccctca tgtaacaaaa ctcgttactg tatcaacata acaataaccc
     7921 cataactaat tagcgagaaa agaatgaaaa tcggctatgc acgtaaa
//
LOCUS       DAECNO010000146         7918 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.146, whole genome shotgun sequence.
ACCESSION   DAECNO010000146 DAECNO010000000
VERSION     DAECNO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.146"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            239..742
                     /gene="dps"
                     /locus_tag="KKO08_003670"
                     /gene_synonym="pexB"
     CDS             239..742
                     /gene="dps"
                     /locus_tag="KKO08_003670"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBD6319754.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1146..1892
                     /gene="glnH"
                     /locus_tag="KKO08_003671"
     CDS             1146..1892
                     /gene="glnH"
                     /locus_tag="KKO08_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBD6319755.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2031..2690
                     /gene="glnP"
                     /locus_tag="KKO08_003672"
     CDS             2031..2690
                     /gene="glnP"
                     /locus_tag="KKO08_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBD6319756.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2687..3409
                     /gene="glnQ"
                     /locus_tag="KKO08_003673"
     CDS             2687..3409
                     /gene="glnQ"
                     /locus_tag="KKO08_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBD6319757.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3670..5894
                     /gene="ybiO"
                     /locus_tag="KKO08_003674"
                     /pseudo
     CDS             3670..5894
                     /gene="ybiO"
                     /locus_tag="KKO08_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5891..6817)
                     /gene="rlmF"
                     /locus_tag="KKO08_003675"
     CDS             complement(5891..6817)
                     /gene="rlmF"
                     /locus_tag="KKO08_003675"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBD6319758.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7093..7353
                     /locus_tag="KKO08_003676"
     CDS             7093..7353
                     /locus_tag="KKO08_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBD6319759.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7618..>7918
                     /locus_tag="KKO08_003677"
     CDS             7618..>7918
                     /locus_tag="KKO08_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBD6319760.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1935 a         2026 c         1978 g         1979 t
ORIGIN      
        1 accgtattta tatatcgaat tatgcaaaag catatttatt ccgaaaattc ctggcgagca
       61 gataaataag aattgttctt atcaatatat ctaactcatt gaatctttat tagttttgtt
      121 tttcacgctt gttaccacta ttagtgtgat aggaacagcc agaatagcgg aacacatagc
      181 cggtgctata cttagtctcg ttaattactg ggacataaca tcaagaggat atgaaattat
      241 gagtaccgct aaattagtta aatcaaaagc gaccaatctg ctttataccc gcaacgatgt
      301 ctccgacagc gagaaaaaag caacagtaga gttgctgaat cgccaggtta tccagtttat
      361 tgatctttct ttgattacca aacaagcgca ctggaacatg cgcggcgcta acttcattgc
      421 cgtacatgaa atgctggatg gcttccgcac cgcactgatc gatcatctgg ataccatggc
      481 agaacgtgcc gtgcagctgg gcggtgtagc tctggggacc actcaagtta tcaacagcaa
      541 aaccccgctg aaaagttacc cgctggacat ccacaacgtt caggatcacc tgaaagaact
      601 ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa gcgattggcg aagcgaaaga
      661 tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac ctggataaat tcctgtggtt
      721 tatcgagtct aacatcgaat aaatccatcg ctgatggtgc agaactttag tacccgataa
      781 aagcggcttc ctgacaggag gccgttttgt tttgcagccc acctcaacgc acttatttag
      841 tgcatccatc tgctatcccc agctgattaa gtaaattttt tgtatccaca tcatcacaca
      901 atcgttacat aaagattgtt ttttcatcag gttttacgct aaataatcac tgtgttgagt
      961 gcacaatttt agcgcaccag attggtgccc cagaatggtg catcttcagg gtattgccct
     1021 ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc aatgagttgc aaaattggca
     1081 cgattttttc atatatgtga atgtcacgca ggggatcgtc ccgtggatag aaaaaaggaa
     1141 atgctatgaa gtctgtatta aaagtttcac tggctgcact gaccctggct tttgcggttt
     1201 cttctcatgc cgcggataaa aaattagttg tcgcgacgga taccgccttc gttccgtttg
     1261 aatttaaaca gggcgataaa tatgtgggct ttgacgttga tctgtgggct gccatcgcta
     1321 aagagctgaa gctggattac gaactgaagc cgatggattt cagtgggatc attccggcac
     1381 tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac catcaccgac gagcgtaaaa
     1441 aagcgatcga tttctctgac ggctactaca aaagcggcct gttagtgatg gtgaaagcta
     1501 acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa agtggttgct gtgaagagcg
     1561 gtactggctc cgttgattac gcgaaagcaa acatcaaaac taaagatctg cgtcagttcc
     1621 cgaacatcga taacgcctat atggaactgg gcaccaaccg cgcagacgcc gttctgcacg
     1681 atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa cggtcagttc aaagcggtag
     1741 gtgactctct ggaagcgcag caatacggta ttgcgttccc gaaaggtagc gacgagctgc
     1801 gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa cggaacttac aacgaaatct
     1861 acaaaaaatg gttcagtact gaaccgaaat aataacgcta cacctgtaaa gcgcactggc
     1921 agttccctct cccccctggg gagaggatta gggtgagggg cgcaaacccg ctccggggcc
     1981 attaattacc ctgaatttga ttatttacac cacggtaaca ggaacaacat atgcagtttg
     2041 actggagtgc catctggcct gccattccgc ttctgattga aggtgccaaa atgaccctgt
     2101 ggatttcggt cctcggtctg gctggcggtc tggtaatcgg attgctggca ggttttgcac
     2161 gcaccttcgg tggttggata gccaaccacg tcgcgctggt ctttattgaa gtgatccgcg
     2221 gcacacctat cgtcgtccag gtgatgttta tctatttcgc cctgccgatg gcgtttaacg
     2281 acttacgcat cgacccattt actgcggcgg tggtcaccat catgatcaac tccggcgcgt
     2341 acattgcgga aatcacgcgt ggtgcggtgc tgtctatcca caaaggtttt cgtgaagcag
     2401 gactggcgct cggtctttca cgttgggaaa ccattcgcta cgtcatttta ccgctggcac
     2461 tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat cagcattaaa gacacctcgc
     2521 tgtttattgt tatcggcgtg gcggaactga cccgtcaggg gcaagaaatt attgccggta
     2581 acttccgcgc ccttgagatc tggagcgccg tggcggtgtt ctatctgatt attaccctgg
     2641 tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa aatcctgtga ttgaatttaa
     2701 aaacgtctcc aagcactttg gcccaaccca ggtgctgcac aatatcgatt tgaacattgc
     2761 ccagggcgaa gtcgtggtga ttatcgggcc gtccggttcc ggtaaatcga ccctgctgcg
     2821 ctgcatcaac aaactggaag aaatcacctc cggcgatctg attgtcgatg gcctgaaggt
     2881 caacgatccg aaagttgacg agcgcctgat tcgccaggaa gcaggtatgg tgttccagca
     2941 gttttacctc ttcccgcatc tgacggcgct gggaaacgtc atgtttggtc cgctacgcgt
     3001 gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt gagctgctgg cgaaagtcgg
     3061 tctggcagaa cgtgcacatc actacccttc cgaactttct ggtggtcaac agcagcgtgt
     3121 ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg ctgtttgatg aaccgacttc
     3181 cgctcttgac ccggaactgc gccatgaagt gctgaaggtt atgcaggatc tggctgaaga
     3241 agggatgacg atggtgatcg tgacccacga aatcggtttt gccgagaaag tagcttcgcg
     3301 gctgatcttt atcgacaaag gccggattgc ggaagatggc aatccgcagg tgttgatcaa
     3361 gaacccgccg agccagcgct tgcaggaatt tttgcagcac gtctcttaat aagacacatt
     3421 gcctgatgcg ctacgcttat caggcctaca ggatatctgg caacttatta aaattgcatg
     3481 aacttgtagg acggataagg cgttcacgcc gcatccggca aaaaagcccg cacgttgtca
     3541 gcaacctgct taatatccct tcctcccttt cacccgaaag ggaggcacac cagattcctc
     3601 tcatttaaaa tcgcccctcc cccagcatct atacttatct ttttgctctg ttttctcact
     3661 ggaggagtca tgcggtggat cctgttcatc ctcttctgcc tgctgggcgc acctgcccac
     3721 gcggtatcca tacccggcgt tacaaccaca acgacaacgg actcaacgac tgaaccggcc
     3781 ccggaaccgg atatcgaaca aaaaaagcgg cctatggcgc actggcggat gtgctggata
     3841 acgacacctc gcgtaaagag ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg
     3901 cggaaccggt cccgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa
     3961 aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt
     4021 atcgcaatat caccggctcc ccgcataagc catttaatcc acaaaccttc agcaatgcgc
     4081 tgagccattt ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt
     4141 gcgcactgcc gctatatcgc aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca
     4201 gcaactggtt gcagcttccg gcgatgatta ttggggcgtt tattatcgac ctgctgttac
     4261 tggccctgac attatttgtc ggccaggtat taagcgacaa cctgaatgca ggcagtcgca
     4321 ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag
     4381 ccgtactacg cctgattttt tgcccaaacg tggcggagct gcgcccgttc acgattcagg
     4441 acgagagcgc ccgttactgg ggtcgtcgcc tgagctggtt aagcagcctg attggctatg
     4501 gcctgattgt ggccgtgccg attatctcta atcaggtgaa tgtacagata ggtgcgctgg
     4561 cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt gtacctgatc ttccgaaata
     4621 aaaaagagat tacccagcat atgctcaact ttgcggagca ttcgctggct tttttcagcc
     4681 tgtttatccg cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcatgc
     4741 tgtttttctt ttcgttgttc gatccgggca acagtctgaa atttatgatg ggtgcaacgg
     4801 tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc
     4861 tggcgaaaac catcaccctc tcgccacata ctcagcgtaa ctatccggag ctacaaaaac
     4921 ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg
     4981 cagtaatgtt gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg
     5041 gcgcggggca gaaaaccgta gatatcctga tccgcatcgc actcattctt ttcttctcgg
     5101 cggttggctg gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg
     5161 gccgcccgct acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg
     5221 cggtgattat cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg
     5281 cgccattgct ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc
     5341 tggtaaaaga tattatcacc ggggtattta ttcagtttga aaacggcatg aacactggag
     5401 atttggtgac tatcgggccg ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg
     5461 gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg
     5521 ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag
     5581 atgctgataa agccaatcag gcactgaaag atgcggtaac ggaattgatg gaaaacgaag
     5641 aaattcgcgg gctgattatt ggtgaaccga actttgccgg gattgtcggc ttaagcaata
     5701 ccgcgtttac actgcatgtt tcgttcacca cgctgccact caaacagtgg acggtacgct
     5761 ttgccctcga cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc
     5821 agacttatca ggtgctgcct gctccgggcg cgaccccggc tgaaccgctg ccgccggggg
     5881 agccaacgct ttaacgttgg cgattaacaa aacggcggcg ctgctcgtcg ttcataaagg
     5941 tccaggcaat aaagcgactc tgcttttgcc cctgggccat ctcttttttt accaccttca
     6001 ccgcgcccac gtcggtcaga gcacgataca acggcggtaa gttttcacca cgagatacca
     6061 gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga
     6121 taaagttgac ttcaccgcct tcacaccaca actcctgttg ctgaccacca aagttcagtg
     6181 catcgtcttt gttcagcccc aggttacggc gtttacgctc actacctgcc cgcgccgcag
     6241 cggcggaatc gtggaatggc gggttacaca aggtcgcgtc gtattgctcg tttttgtgga
     6301 tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa
     6361 gccccggatt agcactgata atcgcctgcg cactgcttaa cgcctggctg ctggtttcac
     6421 taccggtaaa acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac
     6481 caacgccgat atccagaatg ctggcattcg ctggaattgt tccgcttgct tcagccagta
     6541 aatcggcaag gtgatgaata taatccgccc ggcccggtac tggtgggcag agaaaaccgt
     6601 cggggatatc ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct
     6661 tcaccgccag cggattggca aagtctacgc tttgctcccc tgcgggtgta agcgtgagga
     6721 attgcctgag ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac
     6781 ggttgcgcgg atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc
     6841 gtaagatacc cgcataacgc gacagggtaa atatctggct gcgtttatca ttccccaaaa
     6901 ccttaacatg gctatggttt atggcaattc atcataaaaa tcagcattat gcatgtcatt
     6961 gatttgtctt attttgtaaa aatatatcgt tcaaaaaata agcatatctc tgtcaatttt
     7021 ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag gcactacccg ttgtatttat
     7081 gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct gagcggaatc
     7141 tctctcacgg cttatgccgc gcaaccgatg agtaacctgg acagcggtca gctacggccc
     7201 gccggtaccg tttcggcaac tggcgcatca aacctaagcg atctggagga taaactggca
     7261 gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg cggaaatgac
     7321 cagatgttag gtactgcaac catctacaaa taaccccacc aacgtaactg cattcgcctc
     7381 tggatgcagt tttcgcctgt tttgtaatca aaaaacatca aatatgaacg caatgtaaat
     7441 aaatgtattt ctttttcgcg caatgggtga tagaaaatcg catcaagtga taatgcttat
     7501 caaaattact atcacttgca cgaacactat cacgggatta acagtggcat cgcatccgca
     7561 gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg
     7621 gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt
     7681 tgtattggta ttacgcctgt ggcacaggca ctcgccgccg aagggcaagc taacgcggat
     7741 gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat
     7801 ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtgat ttctgaacaa
     7861 gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgt ccccggcg
//
LOCUS       DAECNO010000147         7866 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.147, whole genome shotgun sequence.
ACCESSION   DAECNO010000147 DAECNO010000000
VERSION     DAECNO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.147"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            54..587
                     /gene="ymdB"
                     /locus_tag="KKO08_003678"
     CDS             54..587
                     /gene="ymdB"
                     /locus_tag="KKO08_003678"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBD6319761.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            529..2010
                     /gene="clsC"
                     /locus_tag="KKO08_003679"
     CDS             529..2010
                     /gene="clsC"
                     /locus_tag="KKO08_003679"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBD6319762.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="KKO08_003680"
     CDS             complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="KKO08_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBD6319763.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3569..5104
                     /gene="mdoG"
                     /locus_tag="KKO08_003681"
     CDS             3569..5104
                     /gene="mdoG"
                     /locus_tag="KKO08_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBD6319764.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5097..7640
                     /gene="mdoH"
                     /locus_tag="KKO08_003682"
     CDS             5097..7640
                     /gene="mdoH"
                     /locus_tag="KKO08_003682"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBD6319765.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1940 a         1887 c         2079 g         1960 t
ORIGIN      
        1 taaccaaaaa gcctgctgta cacttaagaa acaagaaggt gtaaggaggc gttatgaaaa
       61 cgcgtattca tgttgtgcag ggtgatatta ccaaactggc cgttgatgtg attgtgaatg
      121 cggctaatcc gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat cgcgcagcgg
      181 gtccggccct gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat tgccctacgg
      241 ggcatgccgt tattacgctt gcaggcgatc ttcccgctaa agccgtagta cacaccgtcg
      301 ggccagtctg gcgtggtggg gaacaaaacg aagaccagct tttgcaggat gcctatctga
      361 atagcctacg actggtggcg gcaaacagct atacgtcagt ggcttttcct gcaatcagta
      421 ctggggttta tagttaccct cgtgcggcag cggctgaaat cgcagttaaa accgtttcag
      481 aatttattac ccgtcacgct ttacccgaac aggtatactt tgtctgttat gatgaagaaa
      541 acgcccacct ctacgaaaga ctccttaccc aacaaggaga tgaatgattt gccccggctg
      601 gcgagcgcgg tgctgccact gtgttcgcaa catcccggtc agtgtggtct tttccctctg
      661 gagaaaagtc tggatgcgtt tgccgcccgg tatcgtctgg ccgaaatggc agagcatacg
      721 ctggatgttc agtattacat ctggcaggac gatatgtcgg ggcggttact gttttccgcc
      781 ctgttagccg cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga caacaatacg
      841 cccggacttg acgacatttt gcgcttgctt gacagtcatc cacgcattga agtccggctt
      901 tttaatcctt tctcgtttcg cttgctacgt ccgcttggat atatcaccga cttttcccgt
      961 ctcaatcgcc ggatgcacaa taaaagtttc actgtcgatg gcgtggttac cctagtggga
     1021 ggacgaaata ttggtgatgc ctattttggt gcaggggaag agccactttt ttcggattta
     1081 gatgtcatgg caataggccc cgtggtagag gatgttgccg atgatttcgc ccgctactgg
     1141 tattgcaaat cggtttcacc cttacagcag gtgctggatg tcccggaagg tgaaatggcg
     1201 gatcgcatcg agttacccgc ctcctggcat aacgatgcca tgacgcatcg ttatttacgc
     1261 aaaatggaat ccagtccatt tataaatcat ctggttgatg gaacgttgcc gcttatctgg
     1321 gcgaagacac gtttattaag tgatgatccg gcgaaagggg agggcaaggc aaaacggcat
     1381 tcactgttac cgcagcgcct gttcgatatc atgggctcac ccagtgaacg catcgatatt
     1441 atctcttcct attttgtacc gacacgcgca ggtgtggcgc aactcttacg gatggtgaga
     1501 aaaggggtaa agattgcgat cctaaccaat tctcttgccg ctaacgatgt tgctgtcgtc
     1561 catgccggat acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga attatatgaa
     1621 ctcaagccga cgcgtgaaca aagcagtacg ttacacgatc gcggcataac cggtaattcc
     1681 ggtgccagcc tgcatgctaa aacttttagc atcgatggta aaacggtgtt tatcggctct
     1741 ttcaatttcg atccgcgttc aacattgctc aatactgaaa tgggcttcgt gatagagagc
     1801 gaaacgctgg cacagttaat tgataaacgc tttattcaga gccagtacga tgcggcctgg
     1861 cagctccgtc tggacaggtg gggacggatc aactgggttg atcgtcatgc aaagaaagag
     1921 gttattctca aaaaagaacc cgccaccagt ttctggaagc gggttatggt cagactggcg
     1981 tcgatattgc ccgtggaatg gttattgtaa atgtggctta acgggctggt gctttatcgt
     2041 tctcacgctt aacaaccggt ttaccagaga acaaaaactt cagtaacggg atgcgtaaat
     2101 gaatttcata cagaattatc gcaatcccta ctacgaatat caggccacag agaaaaccaa
     2161 gccagttgga ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt aacgggtggt
     2221 gaaccagata gataaacagc gatgcgttaa caaaataagt cacccgcgct gactggaagt
     2281 taagcaaacg gtggccaaag gagaagacca cattcaccat ccacaggccg aggaccatgg
     2341 tgatcaccga ctcggtttcg tacatccagg catcgccact gccatagcgc tggttgagta
     2401 aataagcgac aaacgccaat gctgcagcga gggtacagcc acgagacggc gtggtaaaca
     2461 aggctttaag atgagggaaa atgaaagcca gtgcgccgag gataaagaac ggtaaataaa
     2521 atagcgtttg catgacaata aaattgaaca tgccattact cagaatgggt ggatacacaa
     2581 taaaaatcgt tcttcttatt accgcatagc cgatgccgag gcataaaaaa atcaccgata
     2641 gttttaccat cgagaatttt ttattcgttt tatcagaatt ttctaaatta tttctgatgc
     2701 gcttaaatat ccatacacac agcgtcgtca tgactactaa caccagtaaa aaccacaggt
     2761 gtgatattaa ttcccaggcc aacgtattat atttgtcata caatgatagc ccaggccaac
     2821 tttccgcttt ccctttgaca tattgcagca taataaattg cggtaatgtc agtaggggga
     2881 tggctgttaa catcgggata cctacacgtt cgacacgtac tttccaccat tttttcaagg
     2941 gatagcgtaa aaaaagcatg taggaaaagt atccggatat aacgaaaaat acctgcatgc
     3001 ggaacgagtg gatgaagtca ttaaaaaggg ttagccacaa tgacggttcg gcgctattca
     3061 catgccatgt atggctcgaa tagattaaag aaatatgaaa agggatccct aacaacatca
     3121 gccaggcgcg gatggagtcg aggaaatatt cacgttgcgc gggtactgga ttcatatatg
     3181 gttaactaat ctcggatttt tcgtcttatc cctgtcgggt tatgccttta ggcttgttgc
     3241 catagcgaca ccgacctgac cgcgccaggc gcaggcttca aggtttttat gcatagcatc
     3301 atcgctacca ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata aatttgctgg
     3361 caaaccctac acgaagtcga tgcttctgtc tttaggagaa gcacggaaag tgaaaacggt
     3421 tgcaatcagg tgcttaatcc atgagccagc gtgctgaacg ataccgggat tcagttgtcg
     3481 gaatggctgg ttatccatta aaatagatcg gatcgatata agcacacaaa gggggaagtg
     3541 cttactaatt atgaaacata aactacaaat gatgaaaatg cgttggttga gtgctgcagt
     3601 aatgttaacc ctgtatacat cttcaagctg ggctttcagt attgatgatg tcgcaaagca
     3661 agctcaatcc ttagccggga aaggctatga ggcgcctaaa agcaacttgc cctccgtttt
     3721 ccgcgacatg aaatacgcgg actatcagca gatccagttt aatcatgaca aagcgtactg
     3781 gaacaatctg aagaccccat tcaaactcga gttctaccat cagggtatgt acttcgatac
     3841 cccggtcaaa ataaatgaag tgactgccac cgcagtcaaa cgaatcaaat acagcccgga
     3901 ttatttcact ttcggcgatg ttcagcatga caaagacacg gtaaaagacc ttggttttgc
     3961 cggttttaaa gtgctttacc cgatcaacag caaagataaa aacgatgaaa tcgtcagcat
     4021 gctcggggcc agctatttcc gcgtgattgg tgcaggtcag gtttatggcc tttctgcccg
     4081 cggcctagca attgataccg ctttgccatc gggtgaagaa tttccacgct tcaaagagtt
     4141 ctggatcgag cgtccaaaac cgactgataa acgtttaacc atttatgcat tgcttgactc
     4201 gccgcgtgcg acaggtgctt acaaattcgt ggttatgcca gggcgtgaca cggttgtgga
     4261 tgtgcagtcg aaaatctatc tgcgcgataa agtcggcaaa ctgggggttg caccgttaac
     4321 cagtatgttc ctgtttgggc cgaaccaacc gtcgcctgca aataactatc gtccggagtt
     4381 gcacgactct aacggtctgt ctatccatgc tggtaatggc gaatggatct ggcgtccgtt
     4441 gaataacccg aaacatttag cggtcagcag cttctccatg gaaaacccgc gaggctttgg
     4501 tctgttgcaa cgaggtcgtg atttctcccg ctttgaagat ctcgatgatc gttacgatct
     4561 ccgtccaagc gcatgggtga ctccgaaagg ggagtggggc aaaggcagcg ttgagctggt
     4621 ggaaattcca accaacgatg aaaccaacga taacatcgtc gcttactgga cgccggatca
     4681 gctgccggag ccgggtaaag agatgaactt taaatacacc atcaccttca gccgtgatga
     4741 agacaaactg catgcgccag ataacgcatg ggtgcaacaa acgcgtcgtt caacggggga
     4801 tgtgaagcag tcgaacctga ttcgccagcc tgacggtact atcgcctttg tggtcgattt
     4861 taccggcgca gagatgaaaa aactgccaga ggataccccg gtcacagcgc aaaccagcat
     4921 tggtgataat ggtgagatag ttgaaagcac ggtgcgctat aacccggtta ccaaaggctg
     4981 gcgtctggtg atgcgtgtga aagtgaaaga tgccaagaaa atcactgaaa tgcgtgctgc
     5041 gctggtgaat gccgatcaga cgttgagtga aacctggagc taccagttac ctgccaatga
     5101 ataagacaac tgagtacatt gacgcaatgc ccatcgccgc aagcgagaaa gcagcattgc
     5161 cgaagactga tatccgcgcc gttcatcagg cgctggatgc cgaacaccgc acctgggcgc
     5221 gggaggatga ttccccgcaa ggctcggtaa aggcgcgtct ggaacaagcc tggccagatt
     5281 cacttgctga tggacagtta attaaagacg acgaagggcg cgatcagctt aaggcgatgc
     5341 cagaagcaaa acgctcctcg atgtttcccg acccgtggcg taccaacccg gtaggccgtt
     5401 tctgggatcg cctgcgtgga cgcgatgtca cgccgcgcta tctggctcgt ttgaccaaag
     5461 aagagcagga gagcgagcaa aagtggcgta ccgttggtac catccgccgt tacattctgt
     5521 tgatcctgac gctcgcgcaa actgttgtcg cgacctggta tatgaagacc attcttcctt
     5581 atcagggttg ggcgctgatt aatcctatgg atatggttgg tcaggatttg tgggtttcct
     5641 ttatgcagct tctgccttat atgctgcaaa ccggtatcct gatccttttt gcggtactgt
     5701 tctgttgggt gtccgccgga ttctggacgg cgttgatggg cttcctgcaa ctgcttattg
     5761 gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc
     5821 atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct
     5881 tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacacttt gatgtctaca
     5941 ttctgagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc
     6001 ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgt cgccgcgtga
     6061 agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca
     6121 tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttatgcggc ctggtgcgct
     6181 tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggtatgg
     6241 atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag
     6301 ccggtttgca cttctggcaa cttggcgagt cgcactactg gggacataac gcgattatcc
     6361 gcgtgaaacc atttatcgag cactgcgcac tggctccgct gccgggcgaa ggttcctttg
     6421 ccggttcaat cctgtcacat gacttcgtag aagcggcgtt gatgcgccgt gcaggttggg
     6481 gggtctggat tgcttacgat ctcccgggtt cttatgaaga attgccgcct aacttgcttg
     6541 atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc
     6601 tggttaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc
     6661 tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtgcatg
     6721 cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggtg tggccgcagt
     6781 ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga
     6841 agttattgag cattttgctt atctggtgca aaggaacgaa agaatacggc ggcttctggc
     6901 gcgttacatt atcgttgctg ctggaagtgc tgttttccgt gctgctggct ccggtacgca
     6961 tgctgttcca tacggtcttc gttgtcagcg cgttccttgg ctgggaagtg gtgtggaatt
     7021 caccgcagcg tgatgatgac tccacttcat ggggcgaagc gttcaaacgc cacggctcac
     7081 agctgctgtt ggggttagtg tgggctgttg ggatggcgtg gctggatctg cgtttcctgt
     7141 tctggctggc accgattgtc ttctcgttga tcctgtcacc gtttgtttcg gtgatttcca
     7201 gccgtgccac cgttggtctg cgcaccaaac gctggaaact gttcctgatc ccggaagagt
     7261 attcaccgcc gcaggtgctg gttgataccg atcggttcct tgagatgaat cgccaacgct
     7321 cccttgacga tggctttatg cacgcagtgt ttaacccgtc atttaacgct ctggcaaccg
     7381 caatggcgac agcgcgtcac cgcgccagta aggtgctgga aatcgcccgt gaccgccacg
     7441 ttgaacaggc gcttaacgag acgccagaga agctgaatcg cgatcgtcgc ctggtgctgc
     7501 taagcgatcc ggtgacgatg gcccgtctgc atttccgcgt ctggaattcc cctgaaagat
     7561 attcttcatg ggtgagttat tacgaaggga taaagctcaa tccactggca ttgcgtaaac
     7621 cggatgcggc ttcgcaataa aaacgtagtt gcctgatgcg ctacgcttat caggcctaca
     7681 tcgttcctgc aatttattga ttttgcaaga ttttgtaggt cggataaggc gttcacgcca
     7741 catccggcaa aaacaacgag ccaataaaaa taccggcgtt atgccgtaat gccagtcagt
     7801 taagcaactg actggctctt tttcggggct gtggggtatt tccagggcct ctcctttacc
     7861 actctc
//
LOCUS       DAECNO010000148         7743 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.148, whole genome shotgun sequence.
ACCESSION   DAECNO010000148 DAECNO010000000
VERSION     DAECNO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.148"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1013
                     /gene="ydfJ"
                     /locus_tag="KKO08_003683"
     CDS             <1..1013
                     /gene="ydfJ"
                     /locus_tag="KKO08_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HBD6319766.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="KKO08_003684"
     CDS             1102..2562
                     /locus_tag="KKO08_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBD6319767.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="KKO08_003685"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="KKO08_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBD6319768.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="KKO08_003686"
     CDS             complement(2979..3665)
                     /gene="ydfH"
                     /locus_tag="KKO08_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBD6319769.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="KKO08_003687"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="KKO08_003687"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBD6319770.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="KKO08_003688"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="KKO08_003688"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBD6319771.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="KKO08_003689"
     CDS             complement(6727..7245)
                     /locus_tag="KKO08_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBD6319772.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="KKO08_003690"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="KKO08_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBD6319773.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1941 a         1812 c         1954 g         2036 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctagcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc tccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DAECNO010000149         7721 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.149, whole genome shotgun sequence.
ACCESSION   DAECNO010000149 DAECNO010000000
VERSION     DAECNO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.149"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..830)
                     /locus_tag="KKO08_003691"
     CDS             complement(<1..830)
                     /locus_tag="KKO08_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HBD6319774.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="KKO08_003692"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="KKO08_003692"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBD6319775.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="KKO08_003693"
     CDS             complement(2479..2706)
                     /locus_tag="KKO08_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBD6319776.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KKO08_003694"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="KKO08_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBD6319777.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KKO08_003695"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="KKO08_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBD6319778.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KKO08_003696"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="KKO08_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBD6319779.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="KKO08_003697"
     CDS             complement(5528..6511)
                     /locus_tag="KKO08_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6319780.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="KKO08_003698"
     CDS             complement(6557..7381)
                     /locus_tag="KKO08_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBD6319781.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KKO08_003699"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="KKO08_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBD6319782.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcactc a
//
LOCUS       DAECNO010000150         7572 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.150, whole genome shotgun sequence.
ACCESSION   DAECNO010000150 DAECNO010000000
VERSION     DAECNO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.150"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            257..2254
                     /gene="betT"
                     /locus_tag="KKO08_003700"
     CDS             257..2254
                     /gene="betT"
                     /locus_tag="KKO08_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HBD6319783.1"
                     /translation="MSENDTTPKKSTSQVNKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPIGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLAVLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWALLMGVVAIALLIAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDIIQPAMTLVQEELNKQG
                     TISHISDAAEDRIRLEVDLGNELNYIYEVRLRGYNSPTFALAALDNDEQQSEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2318..3595
                     /locus_tag="KKO08_003701"
     CDS             2318..3595
                     /locus_tag="KKO08_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HBD6319784.1"
                     /translation="MISRWKWMLKQTFKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAKVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYVDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSTPVLYLSQGQESSISTDLLETIIVSDVRSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSAYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPGIFAEAAQTLAFETL
                     EHATRADHIDSDRYLIKSIYYNLFSGEDSNKKP"
     gene            4212..5642
                     /locus_tag="KKO08_003702"
     CDS             4212..5642
                     /locus_tag="KKO08_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319785.1"
                     /translation="MSSELIAKPGTGVLSTDTTKFEGYLSYLGLPTENIIASLPERQI
                     IEKNLPTFIDSLPAEVKRDARYLSKFVAGAAIGLFDASLNYVWNEVVLNLREKVVVYG
                     LDMFFDAAVGGGRRELYSTEEDLSGLKDNTLINTCRKLELISDVVYTKLNHILTMRND
                     IGASHPNSYSINAFELLGWLQTCVQDILNDKPSASAIQIKSFLENLKRSTDVLDEATI
                     HSMERPLNDLSLQNTDNLLNSIFGIYTADSTGNVVRKNIALFAPHIWNKSSDNVKYKL
                     GVTLDGYKNNLHNEKHALGIEFFTFCDGNRYQSLDSRIISLDGHADDLLEARYAWDNF
                     YNEPPHMRKILSYLKTENDIPLERVHKLIKTVLICRVGKGIPYNTGVSPAGRPLYDQF
                     FGMLGDQNIINTVIAMHSNEVRVNLDNKYCQQHMVSVLTLLRANARSERIQEIIDFLI
                     ANQTILHKVHNDKRYRDLTKNHISFG"
     gene            5783..6385
                     /locus_tag="KKO08_003703"
     CDS             5783..6385
                     /locus_tag="KKO08_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6319786.1"
                     /translation="MNTSSWNKGRIVGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVTALIKL
                     GNLHSNDFLFRSRVGTNQHISTRQYNRIFHGWIAKLGLDDSLYSTHSMRRTKPYLIYK
                     KTKNLRVIQLLLGHRKLESTVRYLGIEVDDALEISESIEI"
     gene            complement(6482..7291)
                     /locus_tag="KKO08_003704"
     CDS             complement(6482..7291)
                     /locus_tag="KKO08_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin"
                     /protein_id="HBD6319787.1"
                     /translation="MTKISERWKHNGITEGYCNICGKYGLLTKDHVPPKCAITLGPVL
                     QKTVSEFFGIQEPVKPLNAKNGSYFRTICSHCNNKVLGGLDVAIENVTKSFKEQLSRY
                     MNGMNVYPFIRIPFDSISFTKAMIGHVLSATSVEDCKKEPVDSPFYTPLKDYVLGKNS
                     SFEETHDIYYWFYPHRMHISAQSVAFMNEGHVAFICALHFFPIGFIITMKNEGTYPAH
                     STKLELEDKFLTFNMTSINYEYTTFPFVNLKGNQMYAISNGHTCVSYPIIK"
BASE COUNT         2101 a         1617 c         1692 g         2162 t
ORIGIN      
        1 gagcagacgc tggtaattcg tgcctaatat acttcactta acttatatcc cagctttgat
       61 acagatcata caaattaaat gccccagtaa ttggcaggct gagtaactta aaactcctta
      121 gaatatagga tgatttgtat cggcactcat tccaccatgc attccgatta atcccgcata
      181 gccagttgaa ctttgctact ctgtgagagg tagtaccttc tatccagtgc ggatttaatt
      241 aatggaataa atgattatga gtgaaaatga tacaacccca aagaagtcta caagccaggt
      301 taacaaagcg gtattcttta catctgcttt gctaattttc cttcttgtcg cctttgccgc
      361 cgtattcccg gatgtcgccg acaaaaattt taaactactt cagcaacaaa tcttcacgaa
      421 tgccagctgg ttctacatac ttgctgtggc cctgatttta ctgagcgtca cgttccttgg
      481 actctcacgt tacggtgata taaagctggg cccagaccat gcgcagcctg atttcagcta
      541 ccactcctgg ttcgcgatgc ttttttcggc agggatgggg atcggcctga tgttctttgg
      601 cgttgccgaa cctgtgatgc attatctttc gccacctgtc ggcactccag agaccgttgc
      661 ggcagcgaag gaagccatgc gcctgacgtt cttccactgg ggtctgcacg catgggcaat
      721 ttatgccatc gtggcgctga ttctggcctt tttcagttac cgtcacggtc tgcctttaac
      781 gctgcgttct gcgctctatc ccattatcgg cgatcgcatt tacgggccta taggccacgc
      841 ggttgatatc ttcgccgtga taggcactgt ctttggcgtt gcaacatcgc tgggttacgg
      901 tgtattgcag gtgaatgccg gtttgaacca tcttttcggc gtgcccatca atgaaacggt
      961 acaggtcatt ttgatcgtgg tcatcacggg gttagcgacg atttcggtgg tgtccggcct
     1021 ggacaaggga atacgcatcc tgtctgagct taatctgggc ctggcggtgt tgctcctggc
     1081 gctggtcttg tgcctgggtc ccactgtgct tctgctgaag tcatttgtgg aaaatacggg
     1141 tggttatctg tcggaactgg tgagtaaaac gttcaacctt tacgcgtatg aacccaagtc
     1201 gagcaactgg ctggggggct ggacattatt atactgggga tggtggcttt catggtcgcc
     1261 gtttgtaggt atgttcattg cacgcgtttc ccgcggcagg acaattcgtg agttcgtgac
     1321 tggcgtgttg tttgttcccg ccggctttac gctcatgtgg atgacggtgt ttggaaacag
     1381 cgccatctat ctcatcatga accagggcgc cactgacctc gccaataccg ttcagcagga
     1441 tgttgcgctt gccctgttta atttcctgga gcatttcccg ttctcttccg tgctgtcatt
     1501 cattgcaatg gcgatggtca tcgtcttctt tgtaacgtct gctgattcgg gggcaatggt
     1561 tgtggatact ctggcatcag gtggggtggc aaacacgccc gtctggcagc gaattttctg
     1621 ggcattgctc atgggcgttg ttgctattgc cctgctgatc gcaggggggc tgagtgcgtt
     1681 gcaaaccgta acgatagcga gtgcattgcc cttctcagtt atcttattaa tatccatata
     1741 cggactgtta aaagccctgc gtcgggattt gaccaagcgt gaaagcctta gcatggcgac
     1801 tattgctcct acggctgcac gtaacccaat tccctggcag agaaggttac gcaatatcgc
     1861 gtatttgccg aagcgctctc ttgtgaaacg ttttatggac gacattatcc aacccgccat
     1921 gacgctggtt caggaggaac ttaacaagca gggaacgata agccacatca gtgacgccgc
     1981 cgaggatcgt attcgccttg aagtcgattt gggcaacgag ctgaattata tatatgaagt
     2041 gaggcttcgc gggtataact caccgacctt cgcgctcgct gcgctggata atgatgagca
     2101 gcagagtgaa caacatcgat attatcgcgc tgaagtctat ctcaaagagg gcggccaaaa
     2161 ttatgatgta atgggctgga atcaggaaca gctgattaat gacatactgg accagtacga
     2221 aaaacacctg cacttcctgc atctggttcg ttaacagcaa catgccgcca cagaggcggc
     2281 atttatttat cccggctgca atatgaggga atgcagaatg atttcacgct ggaaatggat
     2341 gctgaagcag acatttaaaa aactatggtt cagggcaacg ttattcgcaa ttgtcgcaat
     2401 aataacggcc cttttatcaa ttctttttaa atcaatgata cctgagtcgg tttccgtgaa
     2461 ggttggtgcg gaagcagtcg ataacattct gaacatactg gcatcgagta tgctggcagt
     2521 gaccacattt tcgctgagca tcatggtcac agcctacggt tcagccacta ctaatgtgac
     2581 tcccagagct actcgtttag ttgttgaaga cgtcaccaca caaaatgtac tggccacctt
     2641 catcggttct tttctcttca gtctggtagg gattattgcc ctcaatatgg gagcttatgg
     2701 agaaaggggg agagtcattt tattcattgt aacactggtt gtcattgcct taatcctcat
     2761 cacattgctt cgctggatac agcatttgac ctctctgggg agggttggtg agacaacggc
     2821 aaaagtagaa caggcggcca tcgaaacatt tattgcgaga gcaagaaatc cctgtctcgg
     2881 cggatatcca tggcttgaga acaatgaaca gccgaaagga acggttgcag tttatccgaa
     2941 aaagattggc tatgttgaat atgttgatat ggtgaaactc agcaagctgc tgaccaatga
     3001 tccccgtcat gtatacctcg tggcgcagcc gggcagtttc atacatccgt ccacgccagt
     3061 tttgtacctg agtcagggcc aggagtcatc aatcagcacc gatttacttg agacgattat
     3121 tgtctcggat gtacgttcat ttgctcagga cccccgattt tgtcttagcg tcatggccga
     3181 aatagcctgc cgagcccttt cccccgcagt gaacgatcct ggaaccgcca ttgatgtcat
     3241 tggcagaggt gtacgtatac tttccgctta cgcgcagaat aaatctgatg aaatagaagt
     3301 gaaatatcct tctgtacatg ttgcaccact tcagaataac gatctacttg aagacttttt
     3361 ctcacctgtt gcgcgcgatg gtgccagtat gagggaaatt cagataaggg tccttaaagg
     3421 actgtcgatg ctgagtaagg gttggcctgg aatttttgct gaagccgcac aaaccctagc
     3481 atttgaaaca ttagagcatg ctactcgtgc tgaccatata gattctgaca gatatctaat
     3541 aaaatcaatt tattataatt tatttagtgg cgaagattct aataaaaaac catagttgcg
     3601 gagcaagcga tacggttgtt ggcaagtcaa gggcaaagca ttcgcgtgag agtgggcgtc
     3661 tgctttgtgc caaaagcggg cattgggcgt tgtggtaaat gcctgataat ttcttctttt
     3721 actagcacaa gccgctggat aattcccatg tgtccagctt aagtaaatca agaacaactc
     3781 gcgtactggc tgtataaagt ttgccggacg caaatttatt gatttcagaa aaacctgttg
     3841 aaatagaggt atatcagcgt tagtttgatg tgagtaaaga tcaggaaaga aggcagaaaa
     3901 atgttccact tgcgggcaaa aaagccttct attaaattgt taaggcttta tgaagatcgt
     3961 acattacgaa tcgaattcac catgggtagg gaacatgaaa tgtccaaacc ctaagtgcgg
     4021 gagaaatgac tcctgcttga ttcgggatag gttgtactcg catgaagtat gtattggatc
     4081 taaacccaaa tactggtggc gtttgctcac aaatacctta acaagtttag gtatgggacg
     4141 ctcctaacgt cgcgcccctg ctaaaagcgt tagctgttct ctagccttca accagataag
     4201 gaaataatcg tatgagttca gaattgattg caaaacctgg aacaggggta ctttccacag
     4261 atacaacaaa atttgaagga tatttatcct atcttggttt gccgaccgaa aatataattg
     4321 cctctctccc tgagcgccaa attattgaaa aaaacctccc cacattcata gattctttac
     4381 ctgctgaggt aaaaagagat gctcgatatt tatcaaagtt cgttgctggt gcggctatag
     4441 gactatttga tgcatcgtta aattatgttt ggaatgaagt tgtactgaat ttaagggaaa
     4501 aagtagtagt ttatggcctc gatatgttct ttgatgctgc agttggagga ggacgtagag
     4561 agctttattc taccgaagaa gatctttctg gtttaaaaga caacaccctt attaatactt
     4621 gccgaaaact tgaattaata tctgacgttg tttacaccaa gctcaatcat atattgacta
     4681 tgaggaatga cattggtgct tcgcacccaa attcatactc cataaatgct tttgaacttc
     4741 ttggctggct ccaaacatgc gttcaagata ttttgaacga caaaccgtcc gcgtctgcca
     4801 tacaaataaa atcattcctt gagaatctaa aaagatccac tgatgttctt gatgaagcca
     4861 caattcatag catggaaaga cctttgaatg atctgtcgct tcagaatact gacaatctac
     4921 tcaatagtat tttcggcata tatacagctg attcaacagg aaatgttgtc agaaaaaata
     4981 tcgctctttt tgctccgcat atctggaata aaagcagtga taacgtaaaa tacaagctcg
     5041 gcgtgacgct agacggatac aagaacaatc ttcacaacga gaaacatgct ttaggtattg
     5101 agttttttac gttttgtgat ggtaatcgtt atcaatcatt agactctaga attatatcac
     5161 tggacggcca tgccgacgat ctcttagagg ctaggtatgc ttgggataac ttttataatg
     5221 aaccccctca tatgcggaag attctttcgt atttaaagac tgaaaatgac atacctttag
     5281 agagggttca taagcttata aaaacagttt tgatttgtcg tgttggtaaa ggaataccat
     5341 ataatactgg tgtttctccg gcgggacgac ctttgtatga tcagtttttt ggcatgcttg
     5401 gcgatcaaaa cattatcaat acagttatag caatgcactc taatgaagtg cgagtgaatt
     5461 tagacaacaa atactgccag caacatatgg taagcgtttt gactttgtta agagccaatg
     5521 ctcgtagcga aagaattcaa gaaattatag attttttgat cgccaaccaa acgatcctcc
     5581 ataaagtaca caacgataag agatatagag atctaactaa aaatcacatc agttttggct
     5641 aacaaagcgc tgcacacgga ctaaattaag cttcgctcac tgggacgggc tacagcccgt
     5701 cccttaacca aacgttaatg tctgcaattc gctcataagg gacaaccata ctcaaatctc
     5761 ccacattgca ggagatttga gtatgaacac ttcatcgtgg aacaaaggcc gtatcgtagg
     5821 ccaaaaaaga ccacttcaga tatctcatat ctgggggatc cgaatcaggc ttgaactgga
     5881 aggtaagacg cgcgatttgg ctctgttcaa tatggccctg gacagtaagc tacgaggctg
     5941 cgatctggta aaacttaaag tatctgatgt tgcatatggt agctctgttt caagcagggc
     6001 aacggtgttg cagcagaaaa ccggtagccc tgtccaattt gagataacca aagggacaag
     6061 agaagctgtt actgcattga taaaacttgg taatttacat agtaatgact tcttgtttcg
     6121 atcccgggtc ggtactaacc agcacatatc aacccggcaa tacaaccgga tttttcatgg
     6181 gtggatagca aagcttggtc tcgacgattc gctttacagc acacattcca tgagaagaac
     6241 aaaaccttac cttatctaca agaaaactaa gaatctcagg gtgatccaac ttctgttagg
     6301 ccataggaaa ctggaaagca cagtccgtta tctgggcatt gaagtcgatg atgcgttaga
     6361 gatctctgaa tcgattgaaa tctaaggttg tcagggctgc aatagcagcc ctgtgccaac
     6421 agcggacatt atagatggta tatgtagaaa gtgaacatgc gtagattgta tgatttagcc
     6481 gtcacttaat tattggataa ctcacacaag tatgaccgtt gcttatcgca tacatttggt
     6541 ttcccttaag atttacaaat ggaaatgtcg tatactcata atttatagag gtcatattaa
     6601 atgtaaggaa tttatcttca agttctaatt ttgtagagtg ggcagggtat gttccttcat
     6661 ttttcattgt gataataaaa ccgataggaa aaaaatgtaa agcacaaata aatgccacat
     6721 gtccttcgtt cataaatgct acactttgag cggatatgtg cattctgtgt gggtaaaacc
     6781 agtagtaaat atcatgagtt tcctcaaaac ttgaattttt acccaaaaca taatccttta
     6841 aaggtgtata gaaggggcta tctacgggtt cttttttaca atcttcaact gaagttgctg
     6901 atagcacgtg cccaatcata gccttagtga aagatatgct atcaaaaggt attctaatga
     6961 atggatatac attcatacca ttcatatatc gacttaactg ttctttaaag gactttgtta
     7021 cattttcaat tgcaacatcg agcccaccta ataccttatt gttacagtgg ctgcaaatgg
     7081 ttctgaaata agagccattt ttagcattta atggtttaac tggttcctga ataccaaaaa
     7141 attcgctaac tgttttctgc aaaacagggc ctaaggttat ggcgcacttt ggaggaacat
     7201 gatcttttgt gagcaagcca tatttcccac aaatattgca ataaccttca gtaatgccat
     7261 tgtgcttcca tcgttcacta attttcgtca tttttattcc ctcagtagca taagctatct
     7321 atacgttttt taatcctatc aaagatacaa cttaacgtca ggaaaatgat agtgtttatc
     7381 taagtaatac aattaacttc aacgcgtgac agtctgcttt tcgctcttag cggacattag
     7441 cataggctat ttaccataac gcctcattac gcgtaccgct cagacggcct gagcgcgttt
     7501 ctgacgtatc cccgttaaaa acagtaacaa accacccgaa aatgcacgcc agaaacgcta
     7561 cgtaagaatc ta
//
LOCUS       DAECNO010000151         7554 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.151, whole genome shotgun sequence.
ACCESSION   DAECNO010000151 DAECNO010000000
VERSION     DAECNO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.151"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="KKO08_003705"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="KKO08_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="KKO08_003706"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="KKO08_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBD6319788.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="KKO08_003707"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="KKO08_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="KKO08_003708"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="KKO08_003708"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBD6319789.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="KKO08_003709"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="KKO08_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBD6319790.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KKO08_003710"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="KKO08_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBD6319791.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="KKO08_003711"
     CDS             complement(6510..7202)
                     /locus_tag="KKO08_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBD6319792.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1922 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgt aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcgcact gctctttaac aatt
//
LOCUS       DAECNO010000152         7184 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.153, whole genome shotgun sequence.
ACCESSION   DAECNO010000152 DAECNO010000000
VERSION     DAECNO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.153"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(296..1198)
                     /locus_tag="KKO08_003712"
     CDS             complement(296..1198)
                     /locus_tag="KKO08_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBD6319793.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KKO08_003713"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="KKO08_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBD6319794.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="KKO08_003714"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="KKO08_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBD6319795.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="KKO08_003715"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="KKO08_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBD6319796.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="KKO08_003716"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="KKO08_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBD6319797.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="KKO08_003717"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="KKO08_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7184
                     /locus_tag="KKO08_003718"
     CDS             7090..>7184
                     /locus_tag="KKO08_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HBD6319798.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT         1673 a         1811 c         1878 g         1822 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgct
//
LOCUS       DAECNO010000153         6990 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.155, whole genome shotgun sequence.
ACCESSION   DAECNO010000153 DAECNO010000000
VERSION     DAECNO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.155"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..180)
                     /locus_tag="KKO08_003719"
     CDS             complement(<1..180)
                     /locus_tag="KKO08_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6319799.1"
                     /translation="MIVLILVFRLVIGVQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     MTVSLVARQHGVAASQ"
     gene            291..2723
                     /locus_tag="KKO08_003720"
     CDS             291..2723
                     /locus_tag="KKO08_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBD6319800.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2790..4259
                     /locus_tag="KKO08_003721"
     CDS             2790..4259
                     /locus_tag="KKO08_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBD6319801.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4259..5920
                     /locus_tag="KKO08_003722"
     CDS             4259..5920
                     /locus_tag="KKO08_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBD6319802.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6141..6482
                     /gene="symE"
                     /locus_tag="KKO08_003723"
     CDS             6141..6482
                     /gene="symE"
                     /locus_tag="KKO08_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBD6319803.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6529..6849)
                     /locus_tag="KKO08_003724"
                     /pseudo
     CDS             complement(6529..6849)
                     /locus_tag="KKO08_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2056 a         1669 c         1609 g         1656 t
ORIGIN      
        1 ctggctggct gctacaccat gttgccgggc aacgagggag accgtcatcc ccggttcaaa
       61 gctctgctga acaattgcga tcttttcctg tgtggtacgc cgtctgcgtt tctccggccc
      121 taagacatca atcatctgta ctccaatgac tagtctaaaa actagtatta agactatcac
      181 ttaaataagt gatactggtt gtctggagat tcagggggcc agtctaatca tagccaggct
      241 attgatttta tgtacaatca gaatcgtcct gataaaagcg agaacccccc atggcggaac
      301 tgaacctaag taacctgacg gaagcagaca tcattaccaa atgcgttatg ccagccattc
      361 tcaatgcagg ctgggacaac acaacgcaaa tcagacagga ggtcaaactc cgggacggta
      421 aagtcatcgt acgtggtaaa gttgcggcac gcagaacggt aaaatctgct gacatcgtgc
      481 tgtaccacaa gccaggcatt cccttagccg tgattgaagc aaaagccaac aaacatgaaa
      541 ttggcaaagg gatgcaacag ggcattgaat atgcgcgcct gctggacgtt ccttttgttt
      601 ttgccaccaa cggtgatggc tttatcttcc gcgatgccac cgcagccgaa ggtgaatgcc
      661 ttgaaaagca aataacgctg gatgacttcc cctcccccgc tgaactctgg caaaaattct
      721 gcctctggaa aggatacaca caagctcagc ttccggtgat tactcaggac tattacgacg
      781 atggcagcgg caaatcgcct cgttattatc aacttcaggc aatcaacaaa accattgaag
      841 ccgtctccaa cgggcaaaac cgcgttctgc tggtcatggc gaccggaaca gggaaaacct
      901 ataccgcatt ccagatcatc tggcgcctgt ggaaatcaaa aaataaaaaa cgcattttgt
      961 tccttgccga tcgcaacatt ctggtcgacc aaaccaaaaa taatgatttc cagccatttg
     1021 gtacggcaat gaccaaagtc agcggacgca ccattgatcc cgcttatgaa attcacctcg
     1081 cgctctatca ggctataact ggcccggagg aagaccaaaa agcgtttaaa caagtcgcac
     1141 cagatttctt cgatctgatc gtgatcgacg aatgccatcg cggcagcgca tccgaagaca
     1201 gcgcctggcg agagatcctt gattatttca gttccgccac ccaaattggc ttaaccgcca
     1261 cgccaaaaga gacgcatgaa gtctccagca cggattactt cggcgatccg gtttacgtct
     1321 actcactaaa agaagggatc gaagacggct tcctcgctcc ttataaagtt gtccgtgttg
     1381 atattgatgt cgacctgcaa ggctggcgcc caaccaaagg gcaaactgac ttaaacggcg
     1441 aagtgattga cgatcgtatc tataaccaga aagatttcga tcgcacgatg gtgatcgacg
     1501 aacgcacaga actggttgcc agaaccatta ccgactacct caagcgcacc aatccgatgg
     1561 ataaaaccat cgtcttctgt aacgacatcg atcatgcaga acgtatgcgc cgcgccctgg
     1621 ttaatctcaa cccgcagcag gtgaaaaaga acgacaaata cgtcatgaaa atcaccggcg
     1681 atgatgaaat tggcaaagct cagttggata acttcatcaa cccgaaaaaa ccgtacccgg
     1741 ttatcgcgac cacttcagag ctgatgacca ctggtgtgga tgctaaaacc tgcaaactgg
     1801 tagtactaga ccagaacatc cagtcgatga ccaaattcaa gcaaattatc ggtcgtggta
     1861 cacgcatcga cgaacgttac ggcaaactct ggtttaccat cctcgacttt aaaaaagcca
     1921 ccgaactgtt tgccgatgag cgtttcgatg gcattcccga aaaagtcatg gataccacac
     1981 cagaggatat cgccgatcca gaatctgatt ttgaagagaa actcgaagaa atcagcgaac
     2041 atgacgacga acaggtaaca ggcgttgatg aaccgcctgc gccaccatac caggttaccg
     2101 ataccgatga tgtcggccca tttccggaag aagacgagaa gaaaatccgc aagtttcacg
     2161 tcaacggtgt agcggtgggc gttattgcac agcgcgttca gtattacgac gccgacggta
     2221 aactggttac cgaatccttt aaagattaca cccgcaaaac actgctcaaa gaatatgcct
     2281 cgctggatga ctttacccgc aagtggcagg acgccgatcg caaagaagcg atcattcacg
     2341 agctggagca acaggggatc atctgggaag tactggcaga agaagtcggt aaagatctcg
     2401 acccgttcga catgctttgc cacgtagtgt atggtcagcc gccgttaacc cgcaaagagc
     2461 gcgccgagaa cgtgcacaag cggaactact tcacaaaata ctctgaagca gcgcaagccg
     2521 tgctcgataa tctgctggat aaatacgccg atgcgggcgt acaggagatc gaaagtattc
     2581 aggtgctgaa acttaaacca ttcgacagca tgggcacctt accggagatt attaaaaccg
     2641 gatttggcga ccgtaacggg tataatcagg cgctcagcga gctggaaaac gaaatctacc
     2701 aattaccgcc ccgctctgct taacaactct cttttgcgcc ttctgcaacc atcatgttgc
     2761 agaaggcctt gtttttcatg gaatacaaca tgtctatcag ctcagtaatc aaatcattac
     2821 aagatattat gcgcaaagat gccggtgtgg acggcgatgc gcagcgtctc ggtcagctct
     2881 cctggctgct gtttttgaaa atcttcgatg cccaggaaga ggcgctggaa ctggagcagg
     2941 ataactatca atatccgatc ccacagcgtt atttgtggcg cagttgggcc gccaacgcgc
     3001 agggcattac cggtgattcc ctgctggaat tcgttaatga tgatctgttt ccggcgttga
     3061 aaaacctcac tgcgcctatc gataaaaacc cacgcggcta cgtggtgaag caggcgttca
     3121 gcgatgccta taactatatg aaaaacggta cgctactgcg ccaggtgatc aacaagctga
     3181 acgaaattga ctttaccagc gccagcgaac gccatctgtt tggtgatatt tacgaacaga
     3241 tccttaaaga tctgcaatct gcgggcaatg cgggcgaatt ctatactcca cgcgccgtca
     3301 ctcgctttat ggtggatcgc gttgatccga aactcggcga atccattatg gacccggcct
     3361 gcggtacggg cggttttctt gcctgcgcgt tcgatcatgt aaagaacaaa tacgtgaaga
     3421 gcgtcgccga tcatcagaca ctgcaacaac agatccacgg tgttgagaaa aaacagcttc
     3481 cgcacctgct ggcgaccacc aatatgctgc tgcacggcat tgaagtaccg gtacaaattc
     3541 gtcacgacaa caccctgaat aaaccgcttt cctcctggga tgagcaactg gatgtcattg
     3601 ttaccaaccc gccgtttggt ggcacggaag aagacggtat tgagaagaac tttccggcag
     3661 agatgcaaac ccgcgaaacg gcggatctgt ttctgcaact gattgtggaa gtactggcga
     3721 aaaacggtcg tgcggcggtg gtattgccgg atggcacact atttggcgaa ggtgttaaaa
     3781 ccaaaatcaa aaagctactt accgaagagt gcaacctgca taccatcgtg cgtttaccga
     3841 atggtgtgtt taatccctat accggcatta aaaccaacct gttgttcttt accaaaggtc
     3901 agccaaccaa agagatttgg ttctatgaac atccgtatcc ggcgggcgtg aaaaactaca
     3961 gcaaaaccaa gccgatgaag tttgaagagt ttcaggcgga gatcgactgg tggggtaacg
     4021 aggccgatgg ttttgccagc cgcgtcgaga atgagcaagc gtggaaagtc agcattgatg
     4081 acgtgattgc gcgcaacttc aatctggata ttaaaaaccc acatcaggcg gaaaccgtca
     4141 gccatgatcc ggacgaactg ttagcgcagt atgcaaaaca gcaggcggag atccagacgc
     4201 tgcgtaatca actgcgcgat attcttggcg ctgcgctgtc tggcaaggag gttaactaat
     4261 gagtgtggaa aagctgatcg ttgatcatat ggaaacctgg acctcggcgt tgcaaacccg
     4321 ttccaccgcc gggcgcggca gctccggtaa gattgatttg tatggtatta agaaattgcg
     4381 tgagctgatt ctggaactgg cggtgcgcgg taaactggtg ccgcaggatc cgaatgatga
     4441 accggcgtcg gacctgctga agcgtattgc ggcggaaaaa gcagagctgg tgaagcaagg
     4501 caaaattaaa aagcaaaaac cactgccaga aattagtgag gaagagaagc cgtttgaatt
     4561 gccggaggga tgggagtggg tgcacctccc tgatatttat tgctcaattt ctgagtcatc
     4621 tagaaaaata aaatcctccg aaatactacc tgagggaaaa tatcctgtta tcgagcaaag
     4681 ccaagaattt atttctggtt attgcaacaa tgaatgtctg ttaataaagt taaataatcc
     4741 agttatagtt tttggtgatc acacaaggaa tattaaattt attgattttg attttgttgt
     4801 cggcgctgac ggtgtgaaga ttttatcacc tatattaatt tgtgagcgtt tctttttttg
     4861 gcagttacga tcatttaaat tagatgtaag aggatacgcg agacatttta aagtattaaa
     4921 tagctgctta tttgcattac cccccattgc agaacaagag cgaatagttg aaaaagttag
     4981 ctctttgatg tccctctgcg accaactaga acagcaatcc ctgaccagtc tggacgcaca
     5041 tcagcaactg gttgaaaccc tgttgggaac gcttacagac agccaaaaca ccgcggaact
     5101 ggctgaaaac tgggcgcgta ttagcgagca tttcgacacc ctatttacca ccgaagccag
     5161 cgtggatgcg ttaaaacaga ccattctgca actggccgta atgggcaaac ttgtgccgca
     5221 ggatccgaat gacgaacccg cctctgaact gctcaaacgt attgcgcagg aaaaagctca
     5281 attggtgaaa gaagggaaaa taaaaaaaca aaaaccactg ccgccaatta gcgatgagga
     5341 aaaaccgttt gaacttccgg aagggtggga atggtgtcgt attggtaata tagttaatat
     5401 taaatctgaa ttagtatcac caaaagatta tttaaattta tatcaagtag cacctgatat
     5461 tattgaaaag ggcactggga gagtgatatc aaagagaaca gtgaaagaat caggagtcaa
     5521 agggccaaat agtcgctttt ataaaggaca aattgtttat tctaaaatcc gcccgtcatt
     5581 atccaaagtt tttttagcag agtataatgg tctttgcagt gctgatatgt atccattaga
     5641 ctgttacatt aatccaaatt acttattgaa atatatcctt tccattccat ttcttatgca
     5701 agtcaaaaaa gcagagaaca gaataaaaat gccaaaactt aattctgaca gtttttacaa
     5761 tattattgtt gcgattcccc cctataatga acaacaagca atatttgata agataaattc
     5821 tattgaagca gtatgcaatg gtcttatttc ttatattggg atttatcata aaacccaact
     5881 gcatcttgca gacgcactca ctgacgcagc gataaactaa ttccctcgcc ctcacaaaac
     5941 caataaagcg gctaaatagc cgctttattc acatcaccaa aatttatatt tcacgctaat
     6001 tttcatctta ttttcattaa tttttagctg gactttccct gtatttactg atgatgtatc
     6061 caggtattta gcacggtgcg gatgtgcgcc aacacactcg caccgctaat cacaatcact
     6121 atttatagag aatagtaatc atgactgaca cgcattctat tgcacaaccg ttagaagcag
     6181 aagtctcccc ggcaaataat cgtcaattaa ccgtgagtta tgcgagtcgt tacccggatt
     6241 acagccgtat tcccgccatc accctgaaag gtcagtggct ggaagccgcc ggttttgcca
     6301 ccggcacggc ggtagatgtc aaagtgatgg aaggctgtat tgtcctcacc gcccaacccg
     6361 ccgccgccga agagagcgaa ctgatgcagt cactacgcaa ggtgtgtaag ctgtcggcgc
     6421 gtaaacaaag gcaggtacag gaatttattg gggtaattac aggtaaacag aaagtcgcct
     6481 gatacattga cccggtagcg ccagtgctac cgggtattat tcgccgcgtt ataccaacgc
     6541 gctctccatc tgtttttcaa atgcctgacg aattaatccc ataatgtaag gaagttgtgc
     6601 gaggtcgctg aaaccaattt ctacatcgcc attaccccag cgcccaacat ttgtcacatc
     6661 tttggcaata cctttcggat cgaccagttc atgaaactgc atattaagcg acaatcgcag
     6721 tcggcttttt tgcggcacaa catcaacaaa attcgtttcc gctttaaacg caatatatag
     6781 cttcaatact tcctgcgtga tccctgcgtc caggcgcata acttcatcgc gaagatgatc
     6841 gaataacact gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca
     6901 gaaggagcta ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc
     6961 tgtcagaacg gttcttgaaa atcaactttc
//
LOCUS       DAECNO010000154         6670 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.156, whole genome shotgun sequence.
ACCESSION   DAECNO010000154 DAECNO010000000
VERSION     DAECNO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.156"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(152..2155)
                     /locus_tag="KKO08_003725"
     CDS             complement(152..2155)
                     /locus_tag="KKO08_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319804.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2152..3288)
                     /locus_tag="KKO08_003726"
     CDS             complement(2152..3288)
                     /locus_tag="KKO08_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBD6319805.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3281..5560)
                     /locus_tag="KKO08_003727"
     CDS             complement(3281..5560)
                     /locus_tag="KKO08_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319806.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5571..6659)
                     /locus_tag="KKO08_003728"
     CDS             complement(5571..6659)
                     /locus_tag="KKO08_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBD6319807.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1530 a         1834 c         1787 g         1519 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat taccaactgg
       61 tgactctcac caggggctat ataggatatg cctaataccg tggcgtgagc agtccggaac
      121 tggagtagaa ctcttagtaa aaagcactat ttcatccttg ttgctgaagc atggggaata
      181 attgttcgca aagcaaaaca ccgttattca ttgcttctac ccgtgcctcg ctttctgtat
      241 tacgaaattg tgccaacaca tgtgccagcc gataaaaacc caccgcggtg aggtcattcg
      301 ccagcaactc tgcctgatta atagcgctct gttcctgata gcgccagccg ttatggagca
      361 gttgaataag taacgcctgg cagcgcatca gcaactgatg agcagtagac ggcacaggca
      421 gaacgctggc agaagatagt gataccacca caggcgcagt ttctgcgtcc agcgcccagg
      481 cacgagtctt tgtcatcatt acctgtggtt ccagtgtcaa ttgcccatca acaaaactga
      541 caaagccaga aaccagacac acggggtcgt ctgtttgttg caaaagcgcc gccatgcgtt
      601 caatggcata aggcgcgctg gctgaggctg gcagtgataa cgtcagcaga ttatcttcac
      661 cttcgccgct gattacctgc gcatccagcg tctggcggct gctatcccaa ccgagcgaaa
      721 tacactcagc gaccggcaga ataaataagt tatcgacctg attaagaggc cgtatgcagg
      781 cggggggacg ctggcgtaaa tattcccgca aagccacaat gcccggctgg cgtaacggcg
      841 cgctcaacat ttgccaggca tcaggcgaca gcggtacaac gctgcttaag cggttgcggg
      901 tagctaacag cagctcgcca tcggcactgc gttttgctgc ttgtgaaaca atttgcccac
      961 ccgccagtgc gccagcctga aaactaaaca gccgacgcgt agctgccggt gagtcttcct
     1021 gttcacttcg cggccaactg cgcgaaaggt gcaaaatact gccggtgtcg ggatcggtaa
     1081 accagatgcg taaaccataa tgctcaatat cctgccagca acgcatacct aaagacacca
     1141 gccgcagatg atcaagcttt gcttctccgg caatgccaga gccaacgacc gtgcgccacg
     1201 gcacaggagg aacttcacca acactgtcgc tccgggccat ctcttgtgcg caatttaatc
     1261 gactgtttaa tgccgcaagc tgacgtaagc attctccggc atgatagtgg ctggcgcggg
     1321 cgtggaaggc atcaacgctg gcgcgcagtt gccgtagcga ttcactcacc catcgccagc
     1381 tgcagcgttc cgctgcttgc tgcgcgcggc tgaaagcggc ctcgtagtga ataagcggct
     1441 ggctgatgcc gctcagccat aatgcctggc ttagttgttg aacatattga cgacacgttt
     1501 tgccttcttc gctggcaaac ggatcgtcag atgatgtgac gtgttcgctg cgcatctgcc
     1561 agattaaatg agtcaattcc gcttgctgcg cttttgcctg gacaaaggcc tgtactgcca
     1621 gtacgacatg ttcgcaaagt gtgccttcaa tacaatcaca acgggcgaaa cgaatactgc
     1681 tgcgggaata aaaacgcaca tcgctcatcg gtaagcgggc agagggaatt tcacccggcg
     1741 cacagaacaa ctcaatggtg atgcttttag cgaccagcgc ctgtgcgcgt ttgcgggtgg
     1801 catcgggaag ggtagccagt tcttccagcc agattgccgg atcccattcc tcttcttttc
     1861 ccgtaggctg ggcggtagca caaagtcgtt gataacttaa caccagcatc acgcgatgac
     1921 ggcacatgcc gctggcccca taggtgcact gagcctcttt cagtgcctgg ccgttcgcca
     1981 gctgggtacg gacaccgtca ctgaaggtgg cgattaaagc gctgttctca tggctgattt
     2041 ccgggacgtt gccattttcc agttccttaa ggctgcgctt aacaaaaccg gcattgctta
     2101 acgccgtcag tgcctgcggt gtcagttcta ataattccgg acgtagtgaa ttcatgactg
     2161 aagattctcc gcaagccatg atgccagctc gcccggtgtc atggcggcta tttgtgcgcc
     2221 gacattaatc agcgcctggg ccgtatcgcg gtcatagcaa ggtgttgctg tgctatcgag
     2281 cgctgccagt cccagcactt tgatgccgct ctggacacac tttttcactt ggtgcgtcag
     2341 caatgatgat gaaccccctt cataaaaatc gctcacgagg ataatgacgc tttttgcagg
     2401 ttgttcaata agttgccgac catactccat ggcactggcg atattggttc cgccgcctaa
     2461 ttgcactttc attaataact ctaccggatc ggcaacgtct gccgtgagat caacgacgct
     2521 tgtgtcaaac gccaccagat gggtacgaat gccgggtaac tgccataaac aggccgccat
     2581 caccgcagag tggatcaccg aatcgaccat cgatccgctt tgatcaacca gtaagaccag
     2641 ttgccattgc tcgctttggc gtttaatgcg gctgttaaag cgggggaatt cgatatacaa
     2701 cttgccgtgt tgcgggtgcc agtgttgcag gttggcgcgc agagtacttt tgaaatcaaa
     2761 gtttcgcgcc agtggaataa atgagcggcg acgacgatcg cggacaccag aaaaagcctg
     2821 acgaacttcc tttgccagtc gcgccataat ttcttccaca acctggcgca caatcctgcg
     2881 ggcggcagcc agtacttcgg ggttcatcag atgtttggtg tgcaaaacgg cgcgtagcag
     2941 gctttcggaa ggctgcatac gttccagcac gtcgagattt gtcaccacat cttcaatgcc
     3001 gtagcgcagt acggcatcgc tttccagccg ctcaatcacc tgttgcggaa acagcgtgtg
     3061 aatactgttg atccactcag gagtggtgag atttgagcca cctaatccac tagagcgttc
     3121 accacgctgg agccgttcag gatcgcgccc atacagccac tccagcgcgt gatctatctg
     3181 ccgggcgttg tcatccagcc cacaaagcgt cgtttctgcc gcttcgccaa gaattaatcg
     3241 ccagcgttgt agctcacggg tggtcagaag atcgttcagt tcagacatgg aaaactcccc
     3301 agtgttgcag cgatgccagt gcctgctgtt cgagttgttg atgatgtgca atggcttgcg
     3361 gtggacaatg caacggcatt tgcagtgccg aaacgggaag ttgcgccagt tgataatgct
     3421 caagcacctg atgcgccagc gtcccgcgtt ctcgtggcgg tagccaggcc attgccgcgc
     3481 gtaaatcggg cagggcattg gtaaaatcgg catcacttag ttgatttaaa tgactgctga
     3541 aaccggcgat aaatgccggc tgacaggcca gttgatgacg ggctaatgcc agcaaaccgt
     3601 gcagcgcctc gccggattgt gccggggata actgcgccag catcgtcagc gccgcttcgg
     3661 cactagcgtt gggatgttcc agacgcatca gcgcaccaag agtcgcgccg cgatccagtg
     3721 ccaggacatg aatcgcctga ctgtgtcgct ccagcaaggc taccgccgca gaaagcgaaa
     3781 cgccggggag ttgcacaccg ctatgtagat cgcgcaggat atgacacaac gcttgccagc
     3841 tatgcaagtg agcgtgaaac tccttttcat ccggtctgcc gttggattca cacagccaca
     3901 gcgtgcgatc gatagccgcg cataacgtcg tttgtaatat ctgcgcgcct tgcataccgc
     3961 taatttcatc cagccgccat aaggcatata gcacttccag cgcctggccc atttcggcaa
     4021 attgattttc ctgggcgatt aataatgtga gttgctccag taattgttga ctgaaggacg
     4081 ccagacccgc tgacgccgct tggctaagac atgtggtaat gctgccgatt ccacccgcgt
     4141 ccagcatatc ggcttctaat ttatggcgtg cggcttcctg gagtgtggca ccaaagcagg
     4201 cggcctcaat caatgccgca tgctggctaa gcgggcgggt taatttccag cgttcttcac
     4261 cgttgcctgc aagtgtcagt gtgcttccct gctggcgtac aatccctggg atctccagta
     4321 ttgccagccg atgtaacacc tgactttgcg ctagtccatt ggggttaaag cgatttaagg
     4381 taagctccgc cggaagagag attgcggtac gttccagttc gcaagtgaca tcttttggca
     4441 gaggcggttg tggcgtagaa ggggcaagtt taccgaatcc gtcacccgct aatgtgtcta
     4501 tcaacgttag cagaatcgga tcggtatcgg gatgaataac gccgcgatag ctccacggca
     4561 acggcgcatt cagggcttct ttaatcagcg atcctgctat ggcatccagc cagtcagtgc
     4621 gtaacggtag tgtatgaccg cgcaactgtg ccagtgccat cgcatgcaga tgagcggcag
     4681 ccatatccgc ggtcgaagca ggcaacttgt gctggcgcaa acgggtgaga accgttttta
     4741 gcagttgttc tcccgcctgc tgtaagcccc actgccagca ccagttttgc cagaccgggg
     4801 caggcattcc tgaaaggtat cctgccagca catcaaggcg cttttcactg tagggggtga
     4861 gataacaacc tgtaacggca tctgccagcg acggcaattc tggcgtgtta attacctgcg
     4921 gacattcgcg ccacattttt gccagtgccg gagcgtgcca gccaccgcag acaactaaca
     4981 cgtcgccatt attttgctgc atcgcccatc caatccagcg ggccataaag gcttcgcgct
     5041 gacgatttag agcatcgccg ggggagtcgc cccgcagttg ggcaaaatag tgcgccagtg
     5101 cagagggtaa tgcagtttgc tggctttcat cttcgaacaa gtgatcccac agggtgtcgc
     5161 tgttatccat gcgggtggca cgcagcagca aggtctggct ttcctcttgc gtatcaggcg
     5221 aatcgtcctc ttcttcactc tgcgcccagc aaggcaaatc gatgaagtaa gtttgtgcct
     5281 gaatacgacg atcggcttgt agcgcctgcc actccggcga aaattcagca aatggcgtcc
     5341 aggcaccacg cccgggggct gcaccgtcct gatactggca ataactgtaa atagctaccg
     5401 gaagctggtg ggctaaaaac agttcgtcta cccggtcatt aaaatcagca gggccttcaa
     5461 tcaacacgta tcgtggccgc tggctttcga ttaacgattt caccagccgg gcgcaggccg
     5521 gactatgatg ccggatgccg acaattaacg gctcgctcat gcatctttcc tcacggcagg
     5581 cggtggcggg cttgataata agcctgccag tgagcttctt tatgtgtggc gacacgctgt
     5641 tcgaagtaac ggcgcagacg agcgcgatct tcttcgttat ctttgacgat ggttccggca
     5701 atgcagtcca ccaggtcggc tggctcacct gcgcgattcg ctaaaaacca ggcgcggacg
     5761 cctacggcgt gggccacgtt cacggcttca gcggtggaca taatcgctgt tagtgtatcc
     5821 attgacgttt tcttttcgcc gttggcacgc agatcgcgga aggtgcggac cagtaactcc
     5881 agtaccgcgt caggcacttt atgcggaata ccgctatggg ccagcaaacg cgccgaagca
     5941 ctggcgacaa gctccagctc ctgggcgaaa tccataatcg gaaagacagt ttcaaaatcg
     6001 aagcgacgct ttagcgcagc actcatttca ttgacgccgc gatcgcgggt attggcagtg
     6061 gcaataatat taaagccttc tcgcgcatag agctgactgg cttcaccagt gagttccggc
     6121 accgtcatca ccctgtcaga aagcatacct aacagacagt cttgcacttc cagcggtgta
     6181 cgcgttatct cttcaaaacg gacgattttg ccgtcgcgca ttccctggta aagcggggca
     6241 gggaccagcg cttccgttga tggaccatga ttgataagca gggcgtagtt ccagccgtac
     6301 ttgatttgat cttcggtagt agatgcgccg ccctggatgg ttaacccggc atcgccgcta
     6361 atagaggttg ccagtaattc agacaggaga gattttgcgg tgccaggttc ccccaccaac
     6421 atcaaaccgc gtccggtggc aagagtgact aacatacgtt cgacagcgga gggactaatc
     6481 accactttac ggctaatatt ttgcgcgcta tcgccaagga taaaagcacg ggccgcaggc
     6541 aaacttaact gccaaccggg cgggcaaggt gcgttatcat tttgttttaa ttttgccagt
     6601 tcatcggcgt ataacacagc agcaggcgga cgctgcagat gattattctg cggtgacata
     6661 tattcatccc
//
LOCUS       DAECNO010000155         6666 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.157, whole genome shotgun sequence.
ACCESSION   DAECNO010000155 DAECNO010000000
VERSION     DAECNO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.157"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            22..426
                     /locus_tag="KKO08_003729"
                     /pseudo
     CDS             22..426
                     /locus_tag="KKO08_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(533..1369)
                     /locus_tag="KKO08_003730"
     CDS             complement(533..1369)
                     /locus_tag="KKO08_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBD6319808.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1369..2172)
                     /locus_tag="KKO08_003731"
                     /note="aph(3'')-Ib"
     CDS             complement(1369..2172)
                     /locus_tag="KKO08_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBD6319809.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2233..3048)
                     /gene="sul2"
                     /locus_tag="KKO08_003732"
     CDS             complement(2233..3048)
                     /gene="sul2"
                     /locus_tag="KKO08_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBD6319810.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3378..3554
                     /locus_tag="KKO08_003733"
     CDS             3378..3554
                     /locus_tag="KKO08_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBD6319811.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(3736..4740)
                     /locus_tag="KKO08_003734"
     CDS             complement(3736..4740)
                     /locus_tag="KKO08_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HBD6319812.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(4819..6585)
                     /locus_tag="KKO08_003735"
                     /pseudo
     CDS             complement(4819..6585)
                     /locus_tag="KKO08_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT         1443 a         1854 c         1921 g         1448 t
ORIGIN      
        1 agtattgcaa ggacgcggaa catgcctcat gtggcggcca ggacggccag ccgggatcgg
       61 gatactggtc gttaccagag ccaccgaccc gagcaaaccc ttctctatca gatcgttgac
      121 gagtattacc cggcattcgc tgcgcttatg gcagagcagg gaaaggaatt gccgggctat
      181 gtgcaacggg aatttgaaga atttctccaa tgcgggcggc tggagcatgg ctttctacgg
      241 gttcgctgcg agtcttgcca cgccgagcac ctggtcgctt tcagctgtaa gcgtcgcggt
      301 ttctgcccga gctgtggggc gcggcggatg gccgaaagtg ccgccttgct ggttgatgaa
      361 gtactgcctg aacaacccat gcgtcagtgg gtgttgagct tcccgtttca gctgcgtttc
      421 ctgtttgggg tcgtttgcgg gaaggggcgg aatcctacgc taaggctttg gccagcgata
      481 ttctccggtg agattgatgt gttcccaggg gataggagaa gtcgcttgat atctagtatg
      541 acgtctgtcg cacctgcttg atcgcggccg cgatagctag atcgcgttgc tcctcttctc
      601 catccgcgtt ccaagctgcg gaaaggcacc cataagcgta cgcctggtcg agcaggcgac
      661 gcggatcgac gtccagcgca cgagagaatg cgtccgccat ctgtgcaatg cgtctaggat
      721 cgagacaaag gtcgtctctg tcagccggat cgtagaacat attggcggcg ccaaagccca
      781 cttcaccgac cagaccgacg ggatctatca ccagccagcc gcgactggag aacatgatgt
      841 tttcatgatg cagatcgcca tgtagcccac gcagttccga ggcattgctc atcatttgat
      901 cggctataat cgccgcgtgg acgtagtcag tttgacaacc tgcgttttga tcatcgcgcg
      961 cccgctgaaa caaagctgca aagcgatccc ggatcgggag aagggcagaa ggcaggggtt
     1021 cctcagatgc ggcatacagc ttcgccatta gttccgctgc aatttcggtc gcctggtagt
     1081 cgccgtgctc ggcaacgatg tgagagagca ttcgctcccc ggcatattcg agcaacatca
     1141 gattgttctc acgaccgagc aaccggactg ctcccctccc attgcgccat accagatagt
     1201 cggccccgcg cagttcatca gcaatgtctt ctataggttt caatcccttg acgattgcag
     1261 gagtcccgtc tggcaatgaa actttccaaa cgaggctgga aaaggtgtcc gcaatgagaa
     1321 caggttgcga aacgtgccaa tgagcaggaa aaacaggcgg catgaacatc aaccccaagt
     1381 cagagggtcc aatcgcagat agaaggcaag gcgttcgcgg tcgggggctt cgatccccaa
     1441 tacattgaat aggacagcga aggcgcgctc tgcttcatct ggcgctgccc agttctcttc
     1501 ggcgttagca atcatgagtg ccaaatcggc atagcgatct gctgttccga gccgcccaag
     1561 gtcgatcaga cccgtgcatt gaagagtttt agggtccacc atgaagttcg gcatgcaggg
     1621 atcaccatgg caaacaacca tatcggtgcg ctcttggtcg agccgcaccg gtagctctcg
     1681 ttcgacacga gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc
     1741 gggattgacg gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct
     1801 gcgctcaaac ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat
     1861 tgacggccac gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat
     1921 tgccgttatc accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca
     1981 agccacacct cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg
     2041 gcgggaagca ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc
     2101 accagattct ccgcctctga caggcaacca gtcagaatgc gattcaccaa aaaaaatatt
     2161 agttcgattc aatggaggtt ccttcagttt tctgatgaag cgcgaatata gagaaatatc
     2221 ccgaatgtgc agttaacgaa ttcttgcggt ttctttcagc gccgccaata ccgccagccc
     2281 gtcgcgcaag gggcgcggct cgtgtgtgcg gatgaagtca gctccacctg cggcggcggc
     2341 aagctctgca gcgagtgtcg cggccccgac atcccccgga ccacggcctg tgagcgcgcg
     2401 cagaaaggat ttgcgcgaaa cagacagaag caccggcaaa tcgaagcgca gccgcaattc
     2461 atcgaaccgc gccagcaccg agagcgaggt ttcgggagca gcccccagaa aaaaccccat
     2521 gccgggatca aggacaaggc ggttgcgttt gataccggca cccgtcagcg ccgcgatgcg
     2581 cgcgtcaaag aacgccgcaa tgtgatccat gatgtcgcca gcgggtgcct cgcgccgatc
     2641 tgcctgcccg tcttgcaccg aatgcataac gacgagtttg gcagatgatt tcgccaattg
     2701 cggatagaac gcagcgtctg gaaaaccgcg aatatcattg agataggcca caccacgcga
     2761 caaggcatag gcttgcgtcg cgggttgata actgtcgagc gagacgggaa tgccatctgc
     2821 cttgagcgcg tccagcaccg gcgcgatacg cgcgatttct gtgtcggacg aaacaggcgc
     2881 ggcgtcggga ttgctggatg ccggaccgag gtcgatcaca tctgccccct cggccatcag
     2941 cttacgcgcc tgcgcaatgg ctgcgtctgg cgccagatac cggcctccat cggagaaact
     3001 gtccgaggtt atgttgacga tgccgaaaat gatgagcgat ttattcatgg gggcttctat
     3061 aataataata atcgagcatg agtctcatac ggatgctcgg gtcgaaaggg aatccccagg
     3121 cgagtaacct gtttgcggtg ctccattagc tgcaggagca gaatagcata catctggaag
     3181 caaagccagg aaagcggcct atggagctgt gcggcagcgc tcagtaggca atttttcaaa
     3241 atattgttaa gccttttctg agcatggtat ttttcatggt attaccaatt agcaggaaaa
     3301 taagccattg aatataaaag ataaaaatgt cttgtttaca atagagtggg tatcggagac
     3361 gaagaaggac aaggtacatg ctgaccgata ccaagctgcg caatctcaag cccagggaca
     3421 aactctacaa agtgaatgac cgggaaggtc tctatgtggg ggtcgcctca gaaaacggaa
     3481 tctatggtca ctcccgtttt tgcaacaccg attttgacga caagttggct tgcttgaatc
     3541 tatccggcgt ctgaatggga ttttattccc gcgcctcgat gagttccgcg cctgatgaac
     3601 ctccagaaaa tatacggctt caatgagcct ttccgtttta caggttcctc aacaggccgg
     3661 tgggccgtta gtatcatcaa tatcagtatt cgcaaaacca gatgaatgat tgtttaaact
     3721 ggtgtatttc tgcctttatg cttcgtaagt ttgctgtcgc gccgtcagtg cccaggctat
     3781 tctggccagc ttgtttgcca gagcacaggt gacgacaaag ttgcttttcc gacacaacaa
     3841 ctccctgacc cagtcggcca acttgccaga ctggtgttcc agtttttgta tgaataccct
     3901 ggcacactga accaacaaag ttcggatctt tttgttgccc cgcttgctaa tccctaacaa
     3961 tgtcgtccga cctcccgtgc tgtactgtcg gggtaccagc cctgttgccg ccgcaaagtc
     4021 acggctgctg gcgtactgct tcccgtcgcc aatctcagtt gaaatagtac tggcagtcag
     4081 cgttccaacg cagggaatac tcagcaagcg ctgtccaacc tcatcttcgt ccaactttcg
     4141 tttcaactga gattccagat ctttaatctg ctcaacaaga tagtgataat gctgttgtaa
     4201 tttcagcagt aactggctga gataaagagg caaactactg tcctcaagaa gggtactcag
     4261 tcgactaata acggcagcac ctcgcggaac gctgatacca aattccagca gaaaagcatg
     4321 catctgatta gttgttttca ccttatcctg aaccagggat tcacggacac gatgcagagc
     4381 tcgcattgcc tgctgagatt cggttctggg ctgcacgaaa cgcatagatg gacgtgatgc
     4441 tgcttcacag atagcttcag catcaacgaa gtcatttttg ttgcttttaa cgaatgggcg
     4501 gacaaattgc ggtgatatca gctttggaaa atgccctaac tcttccagct tgcgtgccat
     4561 aaagtgagaa ccgccacagg cttccatcgc gatggttgtt gccgggcatg tcgccagaaa
     4621 ttcgattagc tttggtcggg tgaatttttt acggtaaacg gccttcccac gatgatcctg
     4681 acaatgaata tggaaagagt tcttacccag atcgatacca ataagcgcaa tgttttccat
     4741 gatggttctc cgaatgaaag cctgtcctca gcatagtact gggaaggagg gagtgaccat
     4801 ctcattaaat aaagcacgct aagccggttg cagcggtcgt agcggcctga acttgcccgc
     4861 gccgatcttg gcgctgctgc gccagaggta atcgccggtg aggttgatgt gctcccaacc
     4921 gagcggcgac aggtactgca acagcgcgtc atcaacggca tggccgttgc cacgcagcgc
     4981 gtgcgcagcc cgttccagat agaccgtgtt ccacaacacg acggcagccg ttaccagatt
     5041 gaggccgcta gcccggtagc gctgctgctc gaaactgcgg tcgcggattt cacccaggcg
     5101 gttgaaaaac actgccctgg ccagcgcatt gcgcgcctcg cccttgttca ggccggcatg
     5161 cacgcggcgg cgcagttcca cgctttgcag ccagtccagg atgaacagcg tgcgctcgat
     5221 gcggcccagc tcgcggagcg ccacggccag gccgttctgg cgtgggtagc tgccgagctt
     5281 tcggagcatc agggaggccg tcaccgtgcc ctgcttgatc gaggtggcca gccgcaggat
     5341 ttcgtcccaa tgggcgcgga cgtgcttgat gttgagcgtg ccgccgatca tgggtttcag
     5401 cgcgtcatag gcggcgtcgc ccttcgggat gtagagcttg gtgtcgccca ggtcgcggat
     5461 gcgcggcgcg aagcggaagc ccaggaggtg catcagggcg aagacgtgat cggtgaagcc
     5521 cgccgtgtcg gtgtaatgct cctcgatccg caagtcggac tcgtggtaca gcaggccgtc
     5581 gagcacgtag gtcgaatcgc gcacgccgac gttcacgacc ttggtgtgaa atggcgcgta
     5641 ctggtcagaa atgtgggtgt agaacgtccg ccctgggctg ctcccgtatt tcgggttgat
     5701 gtggccggtg ctctcggcct tgctgccggt gcggaagttc tggccgtccg acgatgaggt
     5761 ggtgccgtcg ccccagtgct cggcgaaggg atggcggaac tgtgcgttga ccagatcggc
     5821 cagcgccgcc ccgtaggttt cgtcgcggat gtgccaggct tgcagccaag ccagcttggc
     5881 gtaggtcgtg ccggggcaag actccgccat cttggtcagg cccaggttga tcgcgtcggc
     5941 gaggatcgtg gtcagcaaca ggttcttgtc tttggccggg tcgcccgatt tcagatgcgc
     6001 gaaatgccga gtgaagcccg tccattcgtc cacctccagc agcagttcgg tgatcttgac
     6061 gtgcggcagg atcattgccg tctggtcgat cagcgcttgg gcggtgtcgg gtaccgccgc
     6121 gtcgagcggc gtgatcttca agcctgactc ggtgatgatg gcgtccggca gctcgttggc
     6181 cgtcgccata cggttgacgg tggcaagctg tgtttccagc agcgtcagcc ggtcgttcag
     6241 gtaccggttg cagtcggtgg ccacggccag cggcaattcg ctggcctgct tgaggctggc
     6301 gaatttcgcg ggcggcacca ggtagtcctc gaagtccttg aactggcgcg acccctgcac
     6361 ccagatgtcg ccggaacgca acgcgttctt catctccgac agcgcgcaca gttcgtagta
     6421 gcgccggtcg atgccggtgt cggtcatgac cagcttctgc cagcgcggct tgatgaactc
     6481 ggtcggcgcg tcggcgggca ccttgcgggc gttgtcgctg ttcatgccgc gcagcacctc
     6541 gatggcgtcg agcacgtcct tcgcggcggg agcggcccgc agctggcact gttgcaaagt
     6601 tagcgatgag gcagcctttt gtcttattca aaggccttac atttcaaaaa ctctgcttac
     6661 caggcg
//
LOCUS       DAECNO010000156         6663 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.158, whole genome shotgun sequence.
ACCESSION   DAECNO010000156 DAECNO010000000
VERSION     DAECNO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.158"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..236)
                     /locus_tag="KKO08_003736"
     CDS             complement(<1..236)
                     /locus_tag="KKO08_003736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319813.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEG"
     gene            complement(322..1203)
                     /gene="narH"
                     /locus_tag="KKO08_003737"
                     /pseudo
     CDS             complement(322..1203)
                     /gene="narH"
                     /locus_tag="KKO08_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
     gene            complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="KKO08_003738"
                     /pseudo
     CDS             complement(1200..4940)
                     /gene="narZ"
                     /locus_tag="KKO08_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(5022..6410)
                     /gene="narU"
                     /locus_tag="KKO08_003739"
     CDS             complement(5022..6410)
                     /gene="narU"
                     /locus_tag="KKO08_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBD6319814.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         1587 a         1746 c         1756 g         1574 t
ORIGIN      
        1 ccttcgtcat caacacgtac aaaccagaag accagctttt tgtttcccgc atccacaaag
       61 aacggaatat tcaggtctgc gcagcattca acggcatcgt caaaactatc aaagcgcaga
      121 acttctgcgt cttcttcgtc aaaaaaatca tcttcgtgaa gcttcacgac atagcgggga
      181 agtttgcttc tttgagaggc gggtttacgt ttacggggtt tagctgaacg ggccatataa
      241 ccacctgaaa gacaatgaca ttgcctgttt ttataacggt aattgcagac catgacaagc
      301 cgcagccgtc aggctgccta ctcgatagag gtcaacttcg cgctcggtgc tcgacgcttc
      361 ctcgatgcgg tcggcgtcgt aaagcagcac gcccagatac cggatgcgac ccacgcaggt
      421 ttctgagcac acggtgggtt ggcctgactc aattcgcgga taacagaaga tacatttttc
      481 tgacttgccg cttttccagt tgaagtagat ttttttgtac ggacaaccgc taatgcacaa
      541 acgccagcca cggcatttat cctgatcaat cagcacaatg ccgtcttctt cgcgtttgta
      601 gatagcgccg cttgggcagg tcgccacgca gctgggattg aggcagtgtt cgcacaggcg
      661 cggcaggtac atcatgaagg tgttttcaaa ctgcccgtac atctcctttt gcatggcctc
      721 gaagttgcgg tcgcgggcac gtttttcaaa ctcgccgccc agcagttctt cccagtttgg
      781 gccccagatc actttgtcca tccgtttgcc gtcaatcagt gaacgcgggc gagcagtagg
      841 gatatgtttg ccttccggtg tgctatgcaa atgttcgtag tcgaaggtga aaggttcgta
      901 gtaatcatca atctgctgcg gcaccaccgg gttggcgaag attttggtta ttacgcccat
      961 cttgctgccc agacgcggac ggatcttgcc attcacatcg cggacccagc cgccttgcca
     1021 ctcttcctga tcttcccagt ttttcggata accaatgccc ggtttggttt cgacgttgtt
     1081 aaaccatgcg tactccatgc cttcgcgtcc ggtccagacg ttgttacagg tcaccgaaca
     1141 ggtatggcag ccgatacatt tatcgaggtt aagcaccatg ccgacttgcg aacgtatttt
     1201 cattttttcg cctcctgtac ctgatcgcga ccttcatcat ccagccagtt aacgttcttc
     1261 atcttgcgga tcatgatgaa ttcgtcgcgg ttcgagccga cggtgccgta gtagttaaag
     1321 ccccaggcca gctgcgcgta accgccaatc atatgcgtcg gtttcgggca aacgcgggtg
     1381 accgagttat gaatgccgcc gcgcatgcca gttacttccg aaccaggaat attcataatg
     1441 cgttcctggg cgtgatacat catggtcatg cccggcggta cacgttggct gaccaccgcg
     1501 cgggcagtca gcgcgccgtt ggcgttaaat acttccaccc agtcgttatc gacaatggtc
     1561 agttctcggg catctgtttc gctgatccag acaatcggtc caccgcgaga gagcgtcagc
     1621 attagcaggt tttcactgta ggttgagtga atgccccatt tctggtgcgg cgtcaggaag
     1681 ttaagtgctt tttcctggaa gccgtttggc ggtatctggc gcatctcact gacgctacgg
     1741 gtgtcgatag gcgggcgata agccaccagg ctttcaccaa aagcacgcat ccacggatga
     1801 tcctgataga gctgctggcg tcccgacagc gtgcgccacg gaattaactc atgaacgttg
     1861 gtgtatcccg cgttatagga tacgtgatcg ctttccagac cggaccatgt ggggctggag
     1921 ataattttac gcggctgcgc ctgaatatcg cgaaagcgaa tcttctcgtc ctctttgtgc
     1981 agcgccagat gggtatgttc gcgcccggtg atctcgccca gcgcctgcca cgctttgact
     2041 gcaacatgac cgttggtttc cggtgccagt gccagaatca cttctgaagc gtcaatggcg
     2101 gtgtcaatca gcggacgccc ctgtgccggg ccatcacgct tggtgtaatt gagtttgccg
     2161 aggaaatcga tttcatcctg ggtattccac gaaatccctt taccgccatt gccaagtttg
     2221 tccatcaatg gcccgagcga ggtaaagcgt tcatacgtag cagggtagtc gcgctccacc
     2281 gccacaatat tcggcgcggt tttacccggg atcagatcgc attcgccttt gcgccagtcg
     2341 agcacttcac acggctgtga gagctctgcc ggagagtcat gcagcagtgg ttgtaatacc
     2401 acgtcggttt ctttgccaag atggcccacg cacacttgcg aaaatgcttt ggcaatacct
     2461 ttgtagattt cccagtcgct gcgtgattcc caggccggat cgaccgccgc agaaagcgga
     2521 tgaataaatg gatgcatatc cgaggtgttc atatcgtctt tttcatacca ggtggcggtg
     2581 ggcagaacga tatcggagaa caggcaggta ctggacatgc ggaagtcgag cgtcaccagc
     2641 aggtcgagct tgccttcaat cgccgcagtt tgccactcga cttcttccgg tttgatcccg
     2701 tcgctggcac cgagttcctc gccctgaatc ccgctttcgg tccccagcag atacttctgc
     2761 atatactcgt ggcctttgcc ggaggagcca agcaggttag agcgccagac aaacaaatta
     2821 cgcggatgat tgctgccgct atctggttgt tcgcaggcca tacgtaaatc gcccgatttc
     2881 agcgcctggg cggtaaattc tgtgggggat aatccggcct tgtcggcttc agctttaatc
     2941 ccgagcgggt tacgccccag ctgcggcgca gaaggtagcc agcccatacg ttcggcgcga
     3001 acgttgaaat caatcagatg accgctgtac ttactggcat cggcgagcgg tgaaagtaac
     3061 tcctgagcag agactttttc atagcgccat tggctggaat gattgtagaa aaacgaggtg
     3121 ctgttcatct ggcgcggtgg tcggttccag tcgagcgcaa aggccagtgg caaccagccg
     3181 gtttgtgggc gcagtttttc ctggccgaca tagtgtgccc agccgccacc gctttgcccg
     3241 acacaaccgc agaagatcag catattgatc atcccgcggt agttcatgtc catgtgatac
     3301 cagtggttaa cacctgcgcc gaggataatc attgagcgcc catgcgtttt atgggcggta
     3361 tcggcaaatt cgcgggcgat ggtttcaata tactggcgcg gtacgccggt aatttgctca
     3421 ccccaggctg gggtgtacgg tttgatttca gcgtaatctt tcgcgctgtt ttcatcttcc
     3481 agcccgcgat cgaggccgta attcgccagt accaaatcat aaacgctgac caccggacag
     3541 gtgttgccat cgactaacgt caggttttta acgggcaatt ggcgcaccag caccggattg
     3601 tgttttacgc tgcgaaaatg cggattttca atgccgccaa agtaggggaa ggccacgcct
     3661 gcaacagcgt catgttgacc gagcagggtt aacgacaatt cggtttccgt accggcggca
     3721 atggattcca gattccattt gcctttttct ccccagcgga aaccaatcga accgttcggc
     3781 actaccaatt cacctgcggt attaactgct acggttttcc actgcggatt gttgctttcg
     3841 cccagtccat ccaccaggtc agatgcgcgg atcatgcgcc cgggaacgta gctaccatcg
     3901 tcgcgaggct ccagcattac cagcatcggc atgtcgctgt agcggcggca gtagttgata
     3961 aagtagtcgc tgggattatc gagatgaaac tcttttaaaa tcacatgccc cattgccatc
     4021 gccagggcgc tatcagtgcc ttgtttcggt gccagccact ggtcgcacaa tttggccact
     4081 tcagagtagt caggggtaat ggcgatggtt ttagtgcctt tgtagcgtac ttcggtaaag
     4141 aagtgggcgt ccggcgtacg tgtctgcggt acgttagacc cccaagcgat gatgtagctg
     4201 gagttatacc agtcggcaga ttccggtacg tcggtttgct cgccccaggt catcggcgag
     4261 gcgggcggca ggtcgcaata ccagtcgtag aaactttaac aggtgccgcc aagcagcgac
     4321 agataacgcg ttccggcggc gtaagaaacc atcgacatcg ccgggatcgg cgagaaaccg
     4381 gcaacgcgat ccgggccgta ggttttgatg gtccagacgt tagcggcggc aatcagctgg
     4441 tttagttcct gccagttgga gcggataaac ccgccgcgcc cacgcacttg tttgtagctc
     4501 aggcactttt gcggatcgtt cataatcgat gcccacgcca gtaccggatc gctgtgttgt
     4561 ttgagggctt cgcgccacag ttcaatcagt cgtttacgaa tgagcgggta tttcaggcgg
     4621 ttagcgctgt aaagatacca ggagtaactt gcgccacgcg ggcagccgcg aggttcatga
     4681 ttgggcaggt cagggcgagt gcgcgggtag tcggtctgtt ggatttccca ggtcaccaga
     4741 ccatttttaa cgtagatttt ccagctacag gagcctgtac agttaacgcc gtgggtggaa
     4801 cgcacgattt tgtcgaactg ccaacgctgg cgatagctgt cctcccagtc gcggttgcta
     4861 tgcatcacct gtccgtgacc atcggcaaag gtttcgccct tttgtttgaa gtagcgaaag
     4921 cgatccaaaa gtttactcat gacatgactc ctgctccagg aatgaattgg gatctgatgc
     4981 cttgtcggat gcgacatggt tgtacatcag gcatcgatct cttatttttg gctgaacttc
     5041 cgccgaccat aaaccagcca ggtcagcagc acacaaacga tgtagaagat taaaaacact
     5101 ttcatcgcgc cgaccggaga gccggtcata ttgagcgaca tgccaaatgc ctgcggaata
     5161 aaaaagccgc ccactgcgcc aatggctgag ataaagccca gcgttgccgc cgtttcggtg
     5221 acggcttctc tctgagcttg ctcatcacta ccgcctttca tcttcacccg ataaatggtt
     5281 atctggcgaa agatgacggc gatcatctgg aaagtagaac cacttcccag acccgcggtc
     5341 agaaacagcc ccataaatac ggcgtaaaag gcgatgaaat taccggagcc tgtgcccggt
     5401 aaggtaagga acagcagggc actgaaaatc gccataaaaa taaagttgat caacgtcacc
     5461 cgcacgccgc cgaatttatc ggaaatagca ccaccaaccg accgcgcgat ggcaccaata
     5521 aatgggccaa agaacgccag gcgcagaata ttcacatccg ggaactgggt ttttgccagc
     5581 atggcaaaac ccgcagaaaa accgataaac gaaccgaagg tggcaaggta aagcaggctc
     5641 agcagccaga gatgcaggcg ttgtaagaca gggagctggt cggcaattga ggcgcgtgaa
     5701 ctggcgatat cattcatccc tgaccaggcg gcgatcgtgg caatcgccag tagcggtacc
     5761 caaatccatg cggcattcgc cagcgacatc accgaaccgt cggcctgcgg tacgccattg
     5821 acgccgagaa aggcaaatac aggtacaaaa atgaccagcg gtgcaaccag ctgcattaca
     5881 cttacaccta agtttcctaa tccgccatta atcccaagag cgctcccttg tttggctttt
     5941 ggaaagaaga aactgatatt gcccatgctc gaagcaaagt ttgcacctgc aaaaccgcat
     6001 agcaaagcga taacgataaa tatcccaaaa ggagtattcg gattttgcac ggcaattccg
     6061 agccagacgc aaggaataat caggattgca gtactaaaaa ccggccatcg gcgtccaccg
     6121 aatataggca ccataaagga gtagggaaca cgcaataatg cgccagaaac ggagggtaat
     6181 gcggttaata aaaagagttg atcggtagta aaattaaaac cgattttatt gagattaacg
     6241 gtaactgcgc taaatagcat ccagacacag aaggcaagaa gtagacaact gactgatatc
     6301 cagaggtttc ttcgagcaat atgttttcct ttattttccc agaaggccgg attttctggt
     6361 ttccagtcgc gtaaaagata acgactattt ttctcatttt gcagtgccat attgttcctc
     6421 atatgcacac attggtaatg aaaaaagaca aaacaggagg taaggcgcaa tagccagtta
     6481 ttagaattaa ggatgaattg ggtgaagtgc tgattgaaag aatagataag aaagcgtaac
     6541 cgcgggggca gaatggggat taagtagcca gatatgtgtt actaaatgta actaacaagc
     6601 taaccttcaa acgggggtta atctttgaac gtatgtcatg tttcaaccct tcagatcgtg
     6661 aat
//
LOCUS       DAECNO010000157         6658 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.159, whole genome shotgun sequence.
ACCESSION   DAECNO010000157 DAECNO010000000
VERSION     DAECNO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.159"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..195)
                     /locus_tag="KKO08_003740"
     CDS             complement(<1..195)
                     /locus_tag="KKO08_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6319815.1"
                     /translation="MPMSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAW
                     IKKYGPDSSTNKEQSDAQAEI"
     gene            complement(266..1480)
                     /gene="rspA"
                     /locus_tag="KKO08_003741"
     CDS             complement(266..1480)
                     /gene="rspA"
                     /locus_tag="KKO08_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBD6319816.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1686..2012)
                     /locus_tag="KKO08_003742"
     CDS             complement(1686..2012)
                     /locus_tag="KKO08_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBD6319817.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2147..2488
                     /locus_tag="KKO08_003743"
     CDS             2147..2488
                     /locus_tag="KKO08_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBD6319818.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2554..3083
                     /gene="speG"
                     /locus_tag="KKO08_003744"
                     /pseudo
     CDS             2554..3083
                     /gene="speG"
                     /locus_tag="KKO08_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(3086..3796)
                     /locus_tag="KKO08_003745"
     CDS             complement(3086..3796)
                     /locus_tag="KKO08_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBD6319819.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3904..4209
                     /locus_tag="KKO08_003746"
     CDS             3904..4209
                     /locus_tag="KKO08_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBD6319820.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4408..4731
                     /locus_tag="KKO08_003747"
                     /pseudo
     CDS             4408..4731
                     /locus_tag="KKO08_003747"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5287..5865
                     /locus_tag="KKO08_003748"
                     /pseudo
     CDS             5287..5865
                     /locus_tag="KKO08_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1768 a         1688 c         1561 g         1641 t
ORIGIN      
        1 gatctcggcc tgagcatctg actgttcttt attagtggaa gaatccggac cgtacttctt
       61 tatccaggca taaaggctgt gggtggtgat atcgagacgt gttgcaacgc tggcaacaga
      121 ataaccgcga tcaacaacct gtttgactgc ttcagtttta aactcttcgg gataacgctt
      181 accgctcatg ggcacctctc tttaagccat cttaaatgac tctgaggtgt ctgttaaacc
      241 cgtggcgatt caattatctt actccttacc tgttccacag cgtgccatct tccagacgtg
      301 cgactggtag ataagcaggt tcatagggat atttcgccgc cagcttttca tcgaattcga
      361 taccaagacc cggtttgtct cccggatgca tatagccgtt atcgaaagtc cagttgtgcg
      421 ggaagacttc gagcatttgt tcggaataac ccatgtattc ctggacaccg aaattgggga
      481 cccacagatc aaagtgcagc gccgcagcca tgcagactgg tgacaaatcg gaaggaccgt
      541 gtgagccagt acgtacctga tacagcgaag caaaatcggc aatccggcgc ataccggtaa
      601 ttccgcctgc atgggtcagc gtggtgcgga tataatcgat gagttgctct tcaatcagtt
      661 gtttgcagtc ccagatgctg ttgaagactt cacccactgc gatgggtgtg acggtatgtt
      721 ggcgaatgag acggaagcat tcttggtttt ccgctggcgt cgggtcttcc atccagaaca
      781 tgcgataatc ttcaatgctt ttaccaaagc gcgccgcttc aataggcgtt aagcgatggt
      841 gcatgtcatg cagcaaatgt tcattaaaac caaacttgtt acgtaccgcg tcaaacaatt
      901 tcggcatgaa atcgaggtat ttctccgtcg accacagctg ctcttccggc cactgtcctt
      961 tggttgcggg ttcataagcc agacctttac ctttcgacat gccgtaggtg gttttcatac
     1021 cagggattcc gcactgcacg cggatggctt tgaatcccag ctcctgatga cgggcataat
     1081 catccagagc ttcatcaata ctgtgaccgg tggtatggca ataaaccatc accccttcac
     1141 gagacgcgcc gccgagtaac tggtaaagcg gcatgttggc agctttggct ttaatatccc
     1201 acagcgccat atcaaccgct gaaatggccg acatcgtaac cggaccgcga cgccagtaag
     1261 cacctttata gaaaaactgc cagatatctt cgatacggtg cgcatcgcga ccaataagcc
     1321 gcggacaaag atgatcctgc aaataagagg ccacggaaag ctcacgtcca ttgagggtgg
     1381 catccccaag gcccgtaata ccgtcctcag tggtgatttt taatgtgacg aaattacgcc
     1441 ccggacaggt aacaaaaact tcagccttta cgatcttcat gttcgattcc ttgcatcgct
     1501 tgtcgtgatg catgaaatct acgcaactga gctactgcca tacaagtatg aagatcgaaa
     1561 aaagccggag tgatcacaaa aaaaggcgta tatttgcgct gtgaatggtt gacaaaagat
     1621 gaaatagaat accttttgtc agctgacact tcctcgtatc ttattgataa aatggattta
     1681 tgttcctacg tgcgccccca gcccgcaaca atgatcaaca ttccgcaaag cgcaatcaac
     1741 gcacccgtcc agtcataaag acttagtttc acgccatcca caacgcgcag ccacatcaat
     1801 gccgtgcaga cataaacgcc accataagcc gcgtaaacac gcccactcgc cgcaggatgc
     1861 aacgttaaca accagacaaa cagcgccagt gaaatccccg ccggaagcaa cagccagata
     1921 ctggcgtttc gttttaacca caaccagggc agaaagcatc caataatttc acacagcgca
     1981 gtagcaaaaa atagtaacgt tgttttaatc atctttgtca cttattgaca tcatgtatag
     2041 ttatagggcg acataatatc atcaatataa acatcctcct ggtacgtttt gcgttcgcag
     2101 tggatggtgt agaatcacct ttaatcattc atacagggaa tgaattatga aaatcactct
     2161 cagcaaacga atcggcctgc tcgcttttct gctgccttgc gcactggcat tgagcacaac
     2221 tgttcatgcc gaaactaaca aactggtgat tgagtctggc gacagtgcac aaagccgcca
     2281 gcacgccgct atggaaaaag agcaatggaa tgacacgcgc aatctgcgcc agaaagtgaa
     2341 taaacgcact gaaaaagagt gggataaagc cgacgccgct tttgataacc gcgataaatg
     2401 tgagcaaagc gccaacatca atgcctactg ggagcccaat actttgcgct gcctggaccg
     2461 tcgaactggc cgcgttatta ccccctaacc tgttattgat ttaaggaatg taaggacacg
     2521 ttatgccaag cgcccacagt taagctacgc ccgctggagc gtgaagattt acgctatgta
     2581 catcaactcg acaataacgc cagtgtgatg cgttactggt ttgaggaacc ctacgaagcc
     2641 tttgttgaac tctctgatct gtatgataag cgcatattca cgatcagagc gaacggcgct
     2701 ttgtggtgga atgtgacggc gaaaaagccg gtctggtgga gctggtggaa attaaccatg
     2761 ttcatcgccg ggcagaattt cagataatta tctccccgga gtatcagggg aaaggtctgg
     2821 caacccgtgc cgccaaatta gcaatggact atggcttcac cgttctcaat ctctataagc
     2881 tgtatctgat cgtcgataaa gagaatgaaa aagcgattca catttaccgc aagcttggct
     2941 ttacggttga aggtgaattg atgcatgagt tttttattaa tggtcaatat cgtaatgcca
     3001 ttcgcatgtg tatattccag catcagtatc tggcagagca caaaacaccg ggtcagactc
     3061 tcctgaagcc gaccgcacaa tagcattaat aataatcgat cgtatttttg atggtgtaaa
     3121 cccgttcgac ggcgggtttt actccttcat caacaatgat cagctgacag tccactgggt
     3181 tggcgtgact gtcatattca cagctctgtt ttacattacc aactcgttga ttatttagta
     3241 aagtaaccgc tgtgtaatct aattttttga tcggatccgt tgatggcgtg gcgctgacag
     3301 ataatgtttt gtcgttactt ttcgtggttt tacccagcgg atacccctga tcatcatagc
     3361 gatattccat ctgcatttgt ttgctgctgg ctttaatcac gaagccatta tcatcggttt
     3421 cccaactcac cccggcagaa ggtaattctg ctagctggca ttttccctgt aaacgtactc
     3481 tcttctccag cgtctcggca tcacggtaat aattggcgtc cagtaccaga gcgaccacgg
     3541 tattattttc cagatccagt aattcgagtg aatcaaaaca gccttcttcc gacaaagtcc
     3601 cagaaacacg tttcgtcact tcaccttgct catccattaa tgtctgagtg aaatctttta
     3661 ccggaccacg cagcggatca aaatcgaatt cattagagaa actggccatc tcaggggtaa
     3721 atgaaagtgt tacctctgtg cggtcacatc ctgtgaggaa tatcgcgagt aagcatggta
     3781 gtaatttgta tttcacaaca gtcaccagag agtagagatg attctcaatc atagtagcaa
     3841 atacagtact ttacacgtta aatgctatgc ttaaagaagt tatcttcgcg taaggagctt
     3901 atgatgaaac tctcaacctg ctgtgccgcg cttctgctcg ccctcgcctc acccgcggta
     3961 ctcgccgcgc cgggatcttg tgagcgcata cagagcgata tatcacagcg cattatcaat
     4021 aatggcgtac ccgaaagcag cttcacgtta agtattgtac ccaatgacca ggttgatcag
     4081 cctgattccc aggtcgtcgg gcattgcgct aatgatacgc ataaaattct ctacaaccgc
     4141 accaccagcg gtaacgtctc tgctcccgcg cagtctaccc aggatggcgc gcctgccgaa
     4201 ccgcagtaac acattatcga ctgaacgccg gatatgacac atccggcgat ttgaacatac
     4261 aacataatct caccttatta ctcataccct tctattgatg tggattaata attcttaacc
     4321 caaaatgggt agactccctc tattgttagc gcgctaaata ttcaatatat aaacatttat
     4381 ataacaataa agaacaggga gtgagttatg tccaaaaatg accgaatggt gggcatcagc
     4441 cgcagaacac tcgttaaatc aaccgcgata ggttctctgg cgctggctgc aggcggtttt
     4501 tctttaccgt tcaccctgcg cagtgcagcg gcagcggtac aacaggccag cgaaaaagtg
     4561 atctggggtt cctgttccgt caactgtggt agccgctgtg cacttcgtct acatgttaaa
     4621 gataatgaag tgacctgggt ggaaactgac aataccggca gcgatgagta cggcaaccat
     4681 caggtacgcg cctgtttgcg cggtcgctcc atccgccggc gtattaatca tcccgatcgc
     4741 ttgaatgacc caatgaaacg cgtgggtaaa cgcggtgaag gcaaattcga acggattagc
     4801 tgggatgaag ccctggatac tatcgccagt agcctgaaaa aaaccgtcga acaatatggc
     4861 aatgaagctg tatatattca gtactcttcg gggattgttg gcggcaatat gacccgctct
     4921 tcgccatcag cctcggcggt caaacgcctg atgaactgct acggcggttc actcaatcag
     4981 tatggctcct acagcaccgc gcaaatttcc tgtgccatgc cctacaccta cggcagtaat
     5041 gatggcaaca gcaccacgga tattgaaaac agcaagctgg tcgtgatgtt tggtaacaac
     5101 ccggcagaaa cccgcatgag cggtggtggc atcacttatc ttcttgaaaa agcgcgcgag
     5161 aaatcgaacg ccaaaatgat tgttatcgat ccgcgatata ccgatacggc tgccggtcgt
     5221 gaagaggaat ggctccctat tcgcccgggc accgatgccg cgctggttgc gggtattgcc
     5281 tgggtattga ttaacgaaaa tctcgttgat caaccttttc tcgataaata ctgcgtcggc
     5341 tatgacgaaa aaaccttacc cgcagatgca cccaaaaatg gtcactataa agcctatatt
     5401 cttggtgaag gtgacgataa aacagcaaaa acgccgcagt gggcttcgca aattaccggt
     5461 atcccggtag accgtatcat caaactggcg cgtgaaattg gcacaacaaa acccgcttat
     5521 atctgctagg gctgggggcc gcaacgccag gcaaacggcg agctgactgc acgcgctatt
     5581 gctatgttac ctattttgac gggcaatgtc ggcatcagcg gcggaaatag tggcgcgcgt
     5641 gaatcgacct ataccattac gatagaacgc ctaccggtgc tggataatcc ggtcaaaacg
     5701 tcaatctcct gcttcagctg gacggatgct atcgatcatg gcccgcaaat gacggcactc
     5761 cgcgacggtg tccggggcaa agataaactg gatgtgccca ttaagttcat ctggaacttc
     5821 aaggacttga agcggaccct tagtaggcct gaggtcctgc attgagtcaa gaccttgtgt
     5881 cattctttgt gccagacttg tgtcattaga cgggatgtct agacggctgg tcagcccccc
     5941 cggtggcctc ggtctggact tgaggtgggc ctatgggggg aagtctgctg gcttaatgta
     6001 ttagatggtc attcacattt ttctgtcaga aaccatcggt cccactgtga gtccaaacct
     6061 aaggacctag aagtccaccc acagccttag acagagtgtc gaacacatcc acaccgagtc
     6121 gaccagagag gaccatgtag agacccacag agaggaacac gagtttatag accagagtca
     6181 ggagggactt caggtctgcg gaagtcatag acataaagcc cctatctgtt atgttatatt
     6241 attatattaa caacaggtcc agaccattgg tccagaccat agttagttaa tcacagtgat
     6301 agcccaccac ctgaagtcct gctgatattc agtccagact tacctctgct catggtctta
     6361 cctccctcgg tctcctgacc ttgggtatcg actacagctt gctgagcgtc atctggagga
     6421 gctacaggag gcgggaccga agccccacct tgagtcccac caccacctgt caggcccttg
     6481 gcgatggacc tgaagccttt actaatgctc ttacccattg gtcttacgct tcggtcttgg
     6541 tgaacttcag gcgtttgact acaggcagga cggcagtagc tgagccagtg cccatcttga
     6601 cggtcgtagc agcttcagcg acaacgaaca ctttaccgag gatttccaag tcgtggga
//
LOCUS       DAECNO010000158         6374 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.160, whole genome shotgun sequence.
ACCESSION   DAECNO010000158 DAECNO010000000
VERSION     DAECNO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.160"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            58..1384
                     /gene="pgaC"
                     /locus_tag="KKO08_003749"
                     /pseudo
     CDS             58..1384
                     /gene="pgaC"
                     /locus_tag="KKO08_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1386..1799
                     /gene="pgaD"
                     /locus_tag="KKO08_003750"
                     /pseudo
     CDS             1386..1799
                     /gene="pgaD"
                     /locus_tag="KKO08_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="KKO08_003751"
     CDS             complement(1849..2637)
                     /gene="phoH"
                     /locus_tag="KKO08_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBD6319821.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="KKO08_003752"
     CDS             complement(3245..4510)
                     /gene="efeB"
                     /locus_tag="KKO08_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBD6319822.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="KKO08_003753"
     CDS             complement(4516..5643)
                     /gene="efeO"
                     /locus_tag="KKO08_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBD6319823.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5701..>6374)
                     /locus_tag="KKO08_003754"
     CDS             complement(5701..>6374)
                     /locus_tag="KKO08_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBD6319824.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1534 a         1680 c         1533 g         1627 t
ORIGIN      
        1 accaacctga aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg
       61 attaatcgca tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta
      121 gcgtactttc actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg
      181 tccattatgt ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg
      241 tggggagaaa acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt
      301 ccctgtttta atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt
      361 tatgagaaca ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc
      421 ctggatcgca tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaacag
      481 gggaaagcca ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc
      541 attgatggcg atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg
      601 tacaacccgc gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg
      661 gtgggtaaaa ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag
      721 cgtatctatg gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc
      781 ctggcagaag tgggttactg gaatgacgat atgatcaccg aagatattga tattagctgg
      841 aagctgcagt tgaatcagtg gacgattttt ttacgagcca cgggcgctgt gctggatatt
      901 aatgcctgaa acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc
      961 agaagtattc ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc
     1021 gctgtttttt gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat
     1081 tatttacgca gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc
     1141 gacacatact gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct
     1201 gatgatcgag aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt
     1261 cccggttatt ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat
     1321 gttgatgcct aaaaagcaac gcgcccgttg ggtaagtcct gatcgcggga ttctgagagg
     1381 ttaatatgaa caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt
     1441 atgttgccac aaccatcttg tagacattat ttgctttgtt catattctta ttcgccatgg
     1501 atctgctgac gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt
     1561 tgctggcagt ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt
     1621 ttcaaaaaca gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct
     1681 tagcaatacc tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact
     1741 tcaccagcca ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa
     1801 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
     1861 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
     1921 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
     1981 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
     2041 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
     2101 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
     2161 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
     2221 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
     2281 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
     2341 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
     2401 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
     2461 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
     2521 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     2581 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     2641 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     2701 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     2761 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     2821 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     2881 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     2941 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     3001 tatattcact tatatttaac gataaacacc catttcggtg acattaattt ctactggaaa
     3061 gatgacagag tgatgacagt gatggaaaaa gctgtgtgct ttccgcagga ttcgcagggc
     3121 tggtaaaatg tgcctcgtgt ttgccggatt cggcgcgaac gccttacccg acctacaagt
     3181 gattaataat ttgtgcacaa aactcatgcc cgataaacct gcgcatcggg catatttgcg
     3241 tcgttcaaac ctgcaatagc gcccttccca gataatcgtt cgcgtccttc actcccggca
     3301 gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga
     3361 gccttttttg tactgtcagg aagccttttt ccagatcgtg ttggtagcag acaaacagca
     3421 accccatatc cagttgtccg gagttggtga cccccaacga ataactgtag ccgcgacgca
     3481 gcatcaggct ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca
     3541 gcgcgatcac cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca
     3601 ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg
     3661 gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac
     3721 cgattgtcca cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact
     3781 tatcattctg gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg
     3841 tctctttgcc tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca
     3901 cactgagcaa atccggcgtg tgtttgatga tatcgcgcag cgcatggttc accgtgtcct
     3961 gggtgttggc gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt
     4021 cgttggggaa acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc
     4081 gctcatcaaa caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt
     4141 agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg
     4201 ctccgccctg actcagaaaa gcaaaacgct gagtcaacaa acgaaacaac cgctcaagat
     4261 cggctttatc gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg
     4321 tcaggatccc tgcctgatgc tcaccataaa acggctgttt ctcattgcgt gcatccggtg
     4381 aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca
     4441 gcgcaccaat ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt
     4501 tatactgcat aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc
     4561 cagcgcagta atcggccctt tcagcgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4621 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4681 atcgactttt gccaacagtt ccgggttggc tttttgcagt tgtggacgca gcagatcgac
     4741 aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg
     4801 atcttcttca ccgctgattt tgctggctgc cacttcctca atcagtccgg ctgcgccgcc
     4861 gaccactttt gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc
     4921 ggtataaagc tgctcagcgt actgatccat ccctttggtg gtgttgtcgc caaacaatgc
     4981 tttttccaga cggtggaaac cagtgaattt tgggtcggcg gctttttgct cgtaatcatc
     5041 ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatacg
     5101 ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt
     5161 aatcgcgtcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata
     5221 cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc
     5281 cgccgttgcc tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt
     5341 catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat
     5401 attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg
     5461 gctatggttc tgaataatga actgtgtttt cccggcgtta accgtaatgg tcatcggttc
     5521 gcactgctta tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt tagccataaa
     5581 agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt
     5641 catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg
     5701 ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc
     5761 cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggtgctt cctgatagcc
     5821 aaaaatccct tccatcagcg tgccaaacag cgagtgagtt gagaggaccg cgctcatatc
     5881 gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc
     5941 agccgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc
     6001 aagattgagg cgaataccgc cccagtagag caggaagccg agcaccacgg cagtagcaag
     6061 accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag
     6121 gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc
     6181 ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgttcca gttgcacttt
     6241 gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat
     6301 caccgccacg ataccttcaa acagttcctg ctctttttgc ggaaattcgc cggtggtttc
     6361 gttaatgaag atcc
//
LOCUS       DAECNO010000159         6356 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.161, whole genome shotgun sequence.
ACCESSION   DAECNO010000159 DAECNO010000000
VERSION     DAECNO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.161"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..371
                     /locus_tag="KKO08_003755"
     CDS             <1..371
                     /locus_tag="KKO08_003755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319825.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="KKO08_003756"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="KKO08_003756"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBD6319826.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="KKO08_003757"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="KKO08_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBD6319827.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="KKO08_003758"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="KKO08_003758"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBD6319828.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="KKO08_003759"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="KKO08_003759"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBD6319829.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="KKO08_003760"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="KKO08_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBD6319830.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..>6356)
                     /locus_tag="KKO08_003761"
     CDS             complement(5096..>6356)
                     /locus_tag="KKO08_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBD6319831.1"
                     /translation="TELIDYLDYGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRD
                     IVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHICEIAGGNLA
                     NTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTE
                     QQASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEI
                     ADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQ
                     AAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGI
                     DQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQT
                     PSRPASEQPPAQPRLRITEQDPNWETF"
BASE COUNT         1440 a         1837 c         1525 g         1554 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga tccagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaggac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgcctg ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagtt
//
LOCUS       DAECNO010000160         6335 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.162, whole genome shotgun sequence.
ACCESSION   DAECNO010000160 DAECNO010000000
VERSION     DAECNO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.162"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(53..532)
                     /gene="xdhC"
                     /locus_tag="KKO08_003762"
     CDS             complement(53..532)
                     /gene="xdhC"
                     /locus_tag="KKO08_003762"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBD6319832.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="KKO08_003763"
     CDS             complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="KKO08_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBD6319833.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="KKO08_003764"
     CDS             complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="KKO08_003764"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBD6319834.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4130..4885
                     /locus_tag="KKO08_003765"
     CDS             4130..4885
                     /locus_tag="KKO08_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBD6319835.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4964..5037
                     /locus_tag="KKO08_003766"
     tRNA            4964..5037
                     /locus_tag="KKO08_003766"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4996..4998,aa:Gly,seq:ccc)
     gene            5152..>6335
                     /locus_tag="KKO08_003767"
     CDS             5152..>6335
                     /locus_tag="KKO08_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBD6319836.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DS"
BASE COUNT         1584 a         1586 c         1639 g         1526 t
ORIGIN      
        1 gttaaacaca taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg
       61 ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat
      121 ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca
      181 gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg
      241 atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg
      301 tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc
      361 cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta
      421 gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa
      481 aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt
      541 tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg
      601 cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga
      661 ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc
      721 atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa
      781 attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc
      841 gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg
      901 aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc
      961 attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat
     1021 tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt
     1081 agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag
     1141 atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg
     1201 tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac
     1261 aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc
     1321 gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc
     1381 gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga
     1441 actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca
     1501 tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact
     1561 tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca
     1621 gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt
     1681 tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt
     1741 gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga
     1801 tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc
     1861 caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc
     1921 gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg
     1981 gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta
     2041 gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg
     2101 caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt
     2161 cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca
     2221 ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt
     2281 tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc
     2341 aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct
     2401 ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac
     2461 actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc
     2521 gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat
     2581 caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag
     2641 ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa
     2701 ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc
     2761 cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca
     2821 tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc
     2881 taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct
     2941 cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta
     3001 cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct
     3061 gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc
     3121 tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg
     3181 cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc
     3241 cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg
     3301 gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc
     3361 gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc
     3421 gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg
     3481 gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt
     3541 gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt
     3601 aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa
     3661 tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa
     3721 tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt
     3781 acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca
     3841 cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc
     3901 gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa
     3961 gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa
     4021 tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag
     4081 aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg
     4141 acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc
     4201 gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt
     4261 gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct
     4321 ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact
     4381 gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac
     4441 cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg
     4501 ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg
     4561 caataaaggg attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg
     4621 aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca
     4681 cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca
     4741 aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg
     4801 cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc
     4861 gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt
     4921 gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg
     4981 gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga
     5041 cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc
     5101 agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag
     5161 ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac
     5221 tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg
     5281 accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca
     5341 atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc
     5401 gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc
     5461 taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg
     5521 ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg
     5581 aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat
     5641 attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta
     5701 aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag
     5761 aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta
     5821 atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag
     5881 gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca
     5941 cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac
     6001 aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt
     6061 aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc
     6121 cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct
     6181 cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg
     6241 gcgagactca atgaaatagc ccaactacag gtcaaagacg tcaggaccac tgaggcggga
     6301 accgtttata ttcatatcaa tgaagacgat agtag
//
LOCUS       DAECNO010000161         6334 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.163, whole genome shotgun sequence.
ACCESSION   DAECNO010000161 DAECNO010000000
VERSION     DAECNO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.163"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            8..439
                     /locus_tag="KKO08_003768"
     CDS             8..439
                     /locus_tag="KKO08_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBD6319837.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="KKO08_003769"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="KKO08_003769"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBD6319838.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="KKO08_003770"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="KKO08_003770"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBD6319839.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="KKO08_003771"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="KKO08_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBD6319840.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="KKO08_003772"
     CDS             complement(4441..5148)
                     /locus_tag="KKO08_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBD6319841.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="KKO08_003773"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="KKO08_003773"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBD6319842.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="KKO08_003774"
     CDS             complement(6006..6209)
                     /locus_tag="KKO08_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBD6319843.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1406 a         1811 c         1649 g         1468 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccaccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gctt
//
LOCUS       DAECNO010000162         6269 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.164, whole genome shotgun sequence.
ACCESSION   DAECNO010000162 DAECNO010000000
VERSION     DAECNO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.164"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..667)
                     /locus_tag="KKO08_003775"
     CDS             complement(<1..667)
                     /locus_tag="KKO08_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HBD6319844.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(657..2414)
                     /locus_tag="KKO08_003776"
     CDS             complement(657..2414)
                     /locus_tag="KKO08_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBD6319845.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2430..3314)
                     /locus_tag="KKO08_003777"
     CDS             complement(2430..3314)
                     /locus_tag="KKO08_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBD6319846.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3325..3930)
                     /locus_tag="KKO08_003778"
     CDS             complement(3325..3930)
                     /locus_tag="KKO08_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBD6319847.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4101..>6269)
                     /locus_tag="KKO08_003779"
     CDS             complement(4101..>6269)
                     /locus_tag="KKO08_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBD6319848.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1836 a         1568 c         1316 g         1549 t
ORIGIN      
        1 atggcagtgc tattttgatt cgacgttgat caagtttctg ataattccaa cgacggtcga
       61 cgggcaccat ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat
      121 gatgctgcca tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccacagactt
      181 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
      241 tatgcagcga aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc
      301 atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcggg tagagaaaaa
      361 aaccgcagca ggccattacc gttgccagaa taagccggtg gacaagtcgg cgttgttcga
      421 atgtcgagct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa
      481 caatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctggggatat
      541 catggctgtt aaggtcctga accgcggtga actgattaag agatccatag gtgaagaaga
      601 aaaacggggc cagcaacaat aaccagccag cgccttgtag caatacgttc cgacgttcag
      661 ttatcacgac gcaccgccat cgaaaccgta aaaatgcccc acacatcaat ccgttgagtg
      721 catttatcaa atccggcatt acgcacgagc gaatccatct ccccttgcga acgcacgcgc
      781 attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt
      841 gggtgccacg gctgcccggt gtagatgaga atgccacccg gttcgatggc attggctaga
      901 cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatatag gccagagaca
      961 atcgccagcg taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc
     1021 tgttcaaaac gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca
     1081 acatttaact cactgtaatc acgtaacaaa atatcgctta cggcaggctc gtttgccagc
     1141 gcatccagta catagcgtcc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct
     1201 ttggcgtgga gatcggcaac ggcttgttta atcaatattt gcaaatgggt tttgcgctgg
     1261 cgaataccgc gccatccaac actgttcagg tagattttgt cgattaaacg cccgaatgcg
     1321 ttactgcctt gcggttgatt ttgatagaca taatccaggg aactgcctga atcaaagccg
     1381 gtactcattc cgagatgcag gcccgcagag tgcgcaccga acaatttcat cgcctttcgc
     1441 ataaagcgat acgctaaatc aaccggcgat aatggcacgg gtccaccaga aaggagccgc
     1501 catcgatccg ctgatggtcc tgtgcggtct tcatgctgat aatcaaattt ttgcgatttg
     1561 ttagcatata agcggcagat aaagctttgc attttttcaa atgcctgcgc cctgttctct
     1621 tcacccaatg tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt
     1681 aatctctgat aaaaatcaat ctgcggttgg cgatgcacca catagtcatc acctgatatc
     1741 agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc agacgtttta
     1801 tagagatcga gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa
     1861 ctcgccaccc gttgccgatc gtgggtcaaa tagcgccctt tcacatagga attaataaaa
     1921 aatagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg cacatacaat
     1981 ttaaccttaa aggccggaga agccagcacc agcccacgaa ttgcaggtgc ataatcatga
     2041 acccatgtgg caacaagcac tgcgccgacg ctttgcgcga tcactaccac ctcttccagt
     2101 ccgacctggc tgtcgctgac agcaaaacgg acaaattcat cgacatcctg cactgagcgc
     2161 ataagagacg ggctataacc acgcggcccc gaattttttc catgcccccg ggcatcccag
     2221 gcataaaaag cagtatcagg catcgccagt tcatcaacga gatgttgtag acgaccagaa
     2281 tgttcatgcc cgcgatgaaa taagacgatg acctttttcg cccctggctg taaagtgggc
     2341 cagtgccgat aatacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagggatg
     2401 cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat aaaataaaaa
     2461 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctagt
     2521 aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat cactacatcg
     2581 ccgcaaaaac cactaatacc aattaacaaa cccgctaata atgcctgtaa tgtatttagc
     2641 ggtgtcagca acggtccaat aatcagtgac gcaatcatgg tggtgattac gccccccagc
     2701 agaccttcca gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
     2761 ttcccccata aatactgtgc aatatcgttt gactcggtta acgccaaaag aaataacacc
     2821 agtaacgccc cgccctggat atttatggtt ggcaacatta atagccaggc ggcatgactc
     2881 aaggcaaaaa cggtcattat ccatccccaa aaaatggcgc tcacggtatg ctgaaaaccg
     2941 gaggggtcac ccacaaaaac tcgccaggtg gctaatatca gaaacccagt caaaggtata
     3001 aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc
     3061 caatataacc aacgcgaaaa atgtacggat ataagcgtac aatattcttt caatgccaga
     3121 aaacttatca gcgtaaaaaa cgtcaacgtc atccagcgtg ggcctgaaat caccagtgaa
     3181 aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacttcatc cccttttctc
     3241 tcaggtaacc gccatagcag aaaacggttt atcagcttgc taaaattaac aacgcgaaaa
     3301 gtgttgccag agatttttcc agcattatct ttcagcgctc ataagaagca cactccgaca
     3361 acgattaatc gcagtacaga taaacagaat tgaagcaatg ctccataaaa gattattcca
     3421 ttgcatccac tgtggataaa tggcaacggc cagaccccac agaccaaata ttaacgcgcg
     3481 atcacttttg ccaaatggac cggcataact gcgaacaccg ttaatcgtct gcgcgagtaa
     3541 accgcaaaac tcggtcaata tggtgcaaaa taacatgagt atcacgagtg atgcgttact
     3601 ttccggtaaa aataaaaacg gtaaatagag cgcaatatcg gaaataacat cgccagtctc
     3661 attcaaaatc gcccccagac gtgtttgctg attgcactca cgcgccaaca tgccatccag
     3721 cgcattgagc gccatacgga taaaaagcac gatgggcaat agcaaaaaga ggatgggttg
     3781 tgccaccaac accaacagca atccggtaaa aagagaaagc gccagtgccg taagagtgat
     3841 gtgattcgct gtaacgtggt gcttataaag ccaaaacatc gtcggcctta acagcgactg
     3901 aaagagcggt tttatctggt agagcgtcat tatcaatcct tgataattcg gtttgcgcta
     3961 tggtgcatca attcccctga cggagatatc gcaacacgcc gcttcattaa aaattcagaa
     4021 tatatcaagg tattgggggc tcactgtcag gaatgattat ccccaataaa tcaagtagtt
     4081 aaaaattatt acgctgttat tcaatacaac tcatcaagtt tcctcatcgc ttgttcccgt
     4141 attgcgcgcg tcttaccgtt aagcgccagc tggcggacta aatccagttc agggttaaca
     4201 agctctaacg ccagcaaagg atcacgtttc agcctcgcct tcaatacatc aactggaaag
     4261 cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc cagtgccgca
     4321 gagggggtgt ggcggttgct aagtgcgccg aacttgaaaa atgtgagatt gcttttcgcc
     4381 agtgtactcc accatatacg atccggttgt tttgccagta catccagttg tgctaacgca
     4441 tcggggtcga gtgcataata aacacgtaat gcttcatcaa cctgcgcacg ggagctattt
     4501 tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcgtgttttg ctgtaattcc
     4561 ttgagaacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa aaagtcccag
     4621 tcttccaaac gtatcagggc ggcggcattt aatagcatct ggtcatcaac ttcgccgtgg
     4681 ctaatcagct cagacaaaat catatcagcg ctggtttcat atgcatctga atctgctttc
     4741 ggattatttt tccgatgcga taaagcacgt tgccaaattt tttgtttcat cgacgtgctc
     4801 agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg tgtcaataac
     4861 cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc gacagcaaaa gtaggcaggg
     4921 gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa tgctaaaaat
     4981 aacacacgta ttagatcatc gtcattctcg aatttttggt ataaataggc tgcaactttg
     5041 tcactctctt gtggcgtcat ccgcaataat tgaccatggt gtttcatctc tgccagcttg
     5101 aacgctaagc ttgtgacaac attgtttgat gaatcgttcg ccaactgcat tgcctgattg
     5161 taagtgagtt gtcctccagc accgacctga cgaatagagg aatcatcact gcggatatag
     5221 ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg aatgatcata
     5281 tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc acgtagcgca
     5341 agattcaacg gaccgacacc atcaggtaga cgactcattc gcgtcattat ttcgacagag
     5401 agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg ctgatcggtc
     5461 aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt ttgttcatca
     5521 ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct tgggagagtt
     5581 cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc aacgttgcct
     5641 tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg aaacagcgct
     5701 tcttttttca tctgattaca gacattccct gccatgcgaa tattgcttcg cttaaaaatt
     5761 tctttcagag catcttcatc atttattaga ccccaataat atgcatcagg atttattatc
     5821 atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac catgaattca
     5881 ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatac cccacgcgca
     5941 ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa taccagtcca
     6001 cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt cgttgccgtc
     6061 acgttaccga gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact
     6121 gtcaaattgc caagaacaat caatttttga ttactctcta tattaccttc taccactaaa
     6181 tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag attcacgcta
     6241 ctgttgatag cttgttcaac ttgttgttt
//
LOCUS       DAECNO010000163         6250 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.165, whole genome shotgun sequence.
ACCESSION   DAECNO010000163 DAECNO010000000
VERSION     DAECNO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.165"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="KKO08_003780"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="KKO08_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBD6319849.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="KKO08_003781"
     CDS             578..1027
                     /locus_tag="KKO08_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBD6319850.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="KKO08_003782"
     CDS             complement(1028..1261)
                     /locus_tag="KKO08_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBD6319851.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KKO08_003783"
     CDS             complement(1347..1520)
                     /gene="ortT"
                     /locus_tag="KKO08_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBD6319852.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="KKO08_003784"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="KKO08_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBD6319853.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="KKO08_003785"
     CDS             complement(2212..3021)
                     /locus_tag="KKO08_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBD6319854.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KKO08_003786"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="KKO08_003786"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBD6319855.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="KKO08_003787"
     CDS             complement(4677..5471)
                     /locus_tag="KKO08_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBD6319856.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="KKO08_003788"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="KKO08_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            5836..6081
                     /locus_tag="KKO08_003789"
                     /pseudo
     CDS             5836..6081
                     /locus_tag="KKO08_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1637 a         1573 c         1419 g         1621 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt atatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcaac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgct aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
     5941 aagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc
     6001 cgggccattg cggggctgaa tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg
     6061 ctgaggtata tgccggcctg acacaggcta agtgtgcccg ggaatcggtg caagtataaa
     6121 aaacagcctg ttgcggtata agaatcgacc atgcagagag agaaaacacc ggagtggagg
     6181 gaaaagcaga aaagctcacg tggcataagg cgtggacagc ggtatcgtct ggtttttcaa
     6241 tttcctatac
//
LOCUS       DAECNO010000164         6212 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.166, whole genome shotgun sequence.
ACCESSION   DAECNO010000164 DAECNO010000000
VERSION     DAECNO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.166"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..378
                     /locus_tag="KKO08_003790"
     CDS             <1..378
                     /locus_tag="KKO08_003790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319857.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="KKO08_003791"
     CDS             complement(454..918)
                     /locus_tag="KKO08_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBD6319858.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KKO08_003792"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="KKO08_003792"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBD6319859.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="KKO08_003793"
     CDS             complement(1787..2476)
                     /locus_tag="KKO08_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBD6319860.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KKO08_003794"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="KKO08_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBD6319861.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KKO08_003795"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="KKO08_003795"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBD6319862.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KKO08_003796"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="KKO08_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBD6319863.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1575 a         1688 c         1547 g         1402 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg ac
//
LOCUS       DAECNO010000165         6052 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.167, whole genome shotgun sequence.
ACCESSION   DAECNO010000165 DAECNO010000000
VERSION     DAECNO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.167"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            430..1338
                     /locus_tag="KKO08_003797"
     CDS             430..1338
                     /locus_tag="KKO08_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBD6319864.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="KKO08_003798"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="KKO08_003798"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBD6319865.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="KKO08_003799"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="KKO08_003799"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBD6319866.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="KKO08_003800"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="KKO08_003800"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBD6319867.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="KKO08_003801"
     CDS             complement(5673..>6052)
                     /locus_tag="KKO08_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319868.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1575 c         1561 g         1425 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagtaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggaa atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgtcaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 ccgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAECNO010000166         6030 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.168, whole genome shotgun sequence.
ACCESSION   DAECNO010000166 DAECNO010000000
VERSION     DAECNO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.168"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            88..864
                     /gene="exuR"
                     /locus_tag="KKO08_003802"
     CDS             88..864
                     /gene="exuR"
                     /locus_tag="KKO08_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBD6319869.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1209..1871
                     /locus_tag="KKO08_003803"
     CDS             1209..1871
                     /locus_tag="KKO08_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBD6319870.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1875..2258
                     /gene="mzrA"
                     /locus_tag="KKO08_003804"
     CDS             1875..2258
                     /gene="mzrA"
                     /locus_tag="KKO08_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBD6319871.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2405..2759
                     /locus_tag="KKO08_003805"
                     /pseudo
     CDS             2405..2759
                     /locus_tag="KKO08_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2797..3102
                     /locus_tag="KKO08_003806"
     CDS             2797..3102
                     /locus_tag="KKO08_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBD6319872.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="KKO08_003807"
     CDS             3105..3509
                     /locus_tag="KKO08_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBD6319873.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="KKO08_003808"
     CDS             3499..3798
                     /locus_tag="KKO08_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBD6319874.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3984..4280
                     /locus_tag="KKO08_003809"
     CDS             3984..4280
                     /locus_tag="KKO08_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBD6319875.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4350..5336
                     /gene="yqjG"
                     /locus_tag="KKO08_003810"
     CDS             4350..5336
                     /gene="yqjG"
                     /locus_tag="KKO08_003810"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBD6319876.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5629..5997
                     /locus_tag="KKO08_003811"
     CDS             5629..5997
                     /locus_tag="KKO08_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBD6319877.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1526 a         1470 c         1519 g         1515 t
ORIGIN      
        1 aatcatcaaa tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc
       61 gtccgcattc cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa
      121 caacttgctg ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa
      181 ctgcctgccg aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa
      241 gccatcatca tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat
      301 gtggtttcca accagccgcg tcatcagcag gcggctgaca ataatatgga attcgccaat
      361 tacggtccgt ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc
      421 gcggcaactc aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg
      481 cgcggcgaac aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc
      541 ctggcgacgc agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc
      601 cataacccgt actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg
      661 tgtgatgacc acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag
      721 ctggcaatgt ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac
      781 gatttcgagt tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat
      841 acggccacca gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt
      901 gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc
      961 ccctgcaatc agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga
     1021 cctttgttac aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt
     1081 tttaacaggg aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca
     1141 gcaataacat tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg
     1201 aataatgaat ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa
     1261 ccctggccaa tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc
     1321 ttgaaaatgg cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg
     1381 gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg
     1441 ccgccagcct cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca
     1501 ccgtacaaaa ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt
     1561 ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac
     1621 tgctgccgac gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact
     1681 ggatgagcgg tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa
     1741 cgccggtatt tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg
     1801 tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa
     1861 atcgggggta agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca
     1921 ctgttttact tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt
     1981 ctacgctggc gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct
     2041 ggcatcacct tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc
     2101 tgttaattac atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa
     2161 cattgcccca tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc
     2221 tgacccggtt acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat
     2281 cactcacttt ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta
     2341 ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc
     2401 aagaatgaaa taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta
     2461 tgccactacc ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga
     2521 aaaacaccaa aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc
     2581 caactgttca gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga
     2641 tgaggtggcg gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgatag
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat ctttccgcca gtcgtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gcgtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccctcaaatt
     3841 ttttgaatat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaatagcg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 acattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgctg acggcatttt
     4141 tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa aacctgacaa
     4201 tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc atcgaccgcc
     4261 tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga ggaggaagct
     4321 cctcgttttt gctattggag gagagaaaaa tgggtcaact gattgacggc gtctggcatg
     4381 acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct tccgcatttc
     4441 gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt tttatcgcag
     4501 agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga
     4561 tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg aacccgctga
     4621 tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga gatacgctct
     4681 atcaacatga atttctgtat cagctttatc tccacgccga tccacactac agcggacgag
     4741 ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac gaatcagcag
     4801 aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg ggtgattact
     4861 acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat gacaccgtta
     4921 acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac gaggcggtgg
     4981 cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat cgttacctga
     5041 ccggcaacca gctaaccgaa gccgatattc gcctgtggac tacgctggtg cgttttgatc
     5101 cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac ctgaatctgt
     5161 atggcttcct gcgcgatatc taccagatgc cggggatcgc cgaaactgtc aatttcgatc
     5221 acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg attatttcaa
     5281 ttggtccgtg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa
     5341 aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat gtcggatgcg
     5401 gcgtgaacgc cttatcaaac ctacaaagcg tttatattca atatgttaca atgaccgcgt
     5461 aggccagata agcgtagcgc atctggcgat tttgcgcttg ccatcagtct tagcgcgcct
     5521 tcacggcact ttattaattc cttgaacgaa tatttactgc catttcattc acgtctattc
     5581 ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat ggactggtat
     5641 ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg
     5701 atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga taaaatgtta
     5761 ggctggcaac gcgctggtgg cgaaggcatc ctgacgataa tctacggtat tctggtgttt
     5821 ttaccgtggt gggcggttca gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg
     5881 gcactgctat tcttaatccc gtttatcggc tggcttatta tcatcgtctt taactgccag
     5941 gcgggtacgc caggcgaaaa ccgctttgga ccagatccaa aactggaaca agaataatga
     6001 ttaagcacct tgtaaaaggt gcttcaaatt
//
LOCUS       DAECNO010000167         5981 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.169, whole genome shotgun sequence.
ACCESSION   DAECNO010000167 DAECNO010000000
VERSION     DAECNO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.169"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..262)
                     /locus_tag="KKO08_003812"
     CDS             complement(<1..262)
                     /locus_tag="KKO08_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBD6319878.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            483..911
                     /gene="uspG"
                     /locus_tag="KKO08_003813"
     CDS             483..911
                     /gene="uspG"
                     /locus_tag="KKO08_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBD6319879.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="KKO08_003814"
     CDS             complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="KKO08_003814"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBD6319880.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="KKO08_003815"
     CDS             complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="KKO08_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBD6319881.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3777..4523
                     /gene="dsbG"
                     /locus_tag="KKO08_003816"
     CDS             3777..4523
                     /gene="dsbG"
                     /locus_tag="KKO08_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBD6319882.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4733..5635
                     /locus_tag="KKO08_003817"
     CDS             4733..5635
                     /locus_tag="KKO08_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6319883.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5782..5981
                     /locus_tag="KKO08_003818"
                     /pseudo
     CDS             5782..5981
                     /locus_tag="KKO08_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1605 a         1440 c         1347 g         1589 t
ORIGIN      
        1 tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt
       61 caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt
      121 tgcctcgata aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa
      181 tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc
      241 cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt
      301 aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt
      361 gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata
      421 tcagggataa atattataat catggagaat gatttgttca tgattaacag ggagaaaggg
      481 ttatgtataa ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag
      541 ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac
      601 tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag
      661 agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg
      721 atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt
      781 tggcagaaga actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga
      841 cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg
      901 tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt
      961 tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat
     1021 cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct
     1081 ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc
     1141 gctgcgaaca cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg
     1201 cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca
     1261 aaaataccgg ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga
     1321 ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg
     1381 acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca
     1441 aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg
     1501 gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcggtcc gtcgcagtgc
     1561 gggcagtagg tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc
     1621 cattttgcac ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt
     1681 tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg
     1741 tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc
     1801 ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca
     1861 ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc
     1921 gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc
     1981 tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc
     2041 atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg
     2101 cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg
     2161 ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc
     2221 gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac
     2281 tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac
     2341 tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc
     2401 aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac
     2461 agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc
     2521 gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc
     2581 atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct
     2641 gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac
     2701 ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga
     2761 atccccggga gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat
     2821 ggcgtgaaag acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt
     2881 tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg
     2941 agctgctttg attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac
     3001 ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc
     3061 ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat
     3121 cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg
     3181 agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc
     3241 agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa
     3301 gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa
     3361 tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt
     3421 gttttcgatg agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat
     3481 caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc
     3541 agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct
     3601 ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac
     3661 atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga
     3721 cagtgatagg ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt
     3781 taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt
     3841 ccccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg
     3901 gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc
     3961 ccgatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta
     4021 acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg
     4081 aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct
     4141 ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt
     4201 ctggcaaagt gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga
     4261 cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct
     4321 ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt
     4381 taagtgacaa tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt
     4441 acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta
     4501 atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt
     4561 ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta
     4621 gaaaaactta atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa
     4681 acattgacta agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa
     4741 tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca
     4801 gcatttaagc attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca
     4861 gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg
     4921 tatcgcgccg acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg
     4981 ccttgaacaa acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta
     5041 cggccctcag ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga
     5101 tgcctctgtc gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact
     5161 gttggttcac cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt
     5221 catttgcatg ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg
     5281 aattactgac aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc
     5341 caaatcagca ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa
     5401 aatttcattt cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt
     5461 actgggaata gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga
     5521 gataaaacta gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc
     5581 tttaaataat ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat
     5641 aactatagac agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa
     5701 cactctgctt tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta
     5761 acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg
     5821 gaagctgcac gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca
     5881 ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa
     5941 atgggcaaaa aaatctgcgt tttgtttatc aactgggagg c
//
LOCUS       DAECNO010000168         5784 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.170, whole genome shotgun sequence.
ACCESSION   DAECNO010000168 DAECNO010000000
VERSION     DAECNO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.170"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..941)
                     /gene="hprS"
                     /locus_tag="KKO08_003819"
     CDS             complement(<1..941)
                     /gene="hprS"
                     /locus_tag="KKO08_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBD6319884.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="KKO08_003820"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="KKO08_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBD6319885.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="KKO08_003821"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="KKO08_003821"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBD6319886.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="KKO08_003822"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="KKO08_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="KKO08_003823"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="KKO08_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBD6319887.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="KKO08_003824"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="KKO08_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBD6319888.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1643 a         1305 c         1196 g         1640 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttat
//
LOCUS       DAECNO010000169         5758 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.171, whole genome shotgun sequence.
ACCESSION   DAECNO010000169 DAECNO010000000
VERSION     DAECNO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.171"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..402)
                     /locus_tag="KKO08_003825"
     CDS             complement(<1..402)
                     /locus_tag="KKO08_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HBD6319889.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(547..1050)
                     /gene="cheW"
                     /locus_tag="KKO08_003826"
     CDS             complement(547..1050)
                     /gene="cheW"
                     /locus_tag="KKO08_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBD6319890.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="KKO08_003827"
     CDS             complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="KKO08_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBD6319891.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3040..3966)
                     /gene="motB"
                     /locus_tag="KKO08_003828"
     CDS             complement(3040..3966)
                     /gene="motB"
                     /locus_tag="KKO08_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBD6319892.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3963..4850)
                     /gene="motA"
                     /locus_tag="KKO08_003829"
     CDS             complement(3963..4850)
                     /gene="motA"
                     /locus_tag="KKO08_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBD6319893.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="KKO08_003830"
     CDS             complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="KKO08_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBD6319894.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5558..>5758)
                     /locus_tag="KKO08_003831"
     CDS             complement(5558..>5758)
                     /locus_tag="KKO08_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBD6319895.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1341 a         1553 c         1407 g         1457 t
ORIGIN      
        1 taacgctgtg taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc
       61 aggtaacggt gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt
      121 cctagcgcta tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat
      181 catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga
      241 cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg
      301 gtgaagggaa gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag
      361 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
      421 caagttgatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc
      481 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg
      541 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      601 cagtttttcg atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag
      661 atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa
      721 tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      781 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      841 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      901 gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat
      961 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
     1021 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
     1081 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
     1141 gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta
     1201 ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac cagcaaggcg
     1261 tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg
     1321 cccgcgacgt tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc
     1381 agcacccgct cgccgccggc cagtggatgg agatcggctt cacggggttg cagtgattcc
     1441 ataacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg
     1501 aggatggcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac
     1561 tggatttcga catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg
     1621 ccgcgcccgg agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc
     1681 attgcaactt cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt
     1741 gccagaattc gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg
     1801 ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc
     1861 cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg
     1921 tcgataatgc gttctatcag gctcttgtcg agctcggtgg aactgcccac cagcgtcagt
     1981 tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag
     2041 acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta
     2101 cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg
     2161 gaacgctggg caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga
     2221 tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc
     2281 cgctccacgc ggccggttgg cgcttgttcg gctgccagtt taagcacagg tggggtagct
     2341 atttttggcg aaacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag
     2401 agtaccgctg tgatgtcatc ttcggcgata tcgcccggta atattgccga gagagaatcc
     2461 gcccctttca ccacgtcatt taacgttgtc agatgcccca gctcttcttc cagcaggtcg
     2521 acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gcaactgact gcgactttgc
     2581 tcatcttgcg gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg
     2641 cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgta gctggcggca
     2701 tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc
     2761 aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc
     2821 aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct
     2881 cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc
     2941 ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa
     3001 aatgtctgat aaaaatcgct tatatcgatg ctcacgctgt cacctcggtt cggctgatgg
     3061 cattgtggga acactgacct gtggtgcaac ctcaggtttt tctagggcgc ttactggctc
     3121 attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa
     3181 caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc
     3241 cgccatgccg acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg
     3301 gcgggatgca ttggcccgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct
     3361 ggcgtaggga aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag
     3421 cacaggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt
     3481 tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag
     3541 atcgattttg agatggagac gtaacgcccg cagtttcgga tcggactcta tcaactggtc
     3601 gagatcaccc cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat
     3661 gttcggctgc ttattcactt ccccctggct ctgggtgtaa tcatcaccac cgccgggaat
     3721 tgggctttca ctattggaaa tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg
     3781 gaagtactcc gcaatctgaa tcaactcttt tgggctggag atggagatca gccacatcac
     3841 caaaaaaaag gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg
     3901 tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt
     3961 cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct
     4021 tccagttcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg
     4081 gcgataggcg gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc
     4141 attttgctgg tttcggcgct tttctgacgt aaaacactcg ctagtgggga aataaatccg
     4201 taagccaata aaataccgag gaaagtcccc accatcgcat gtgcgataag cacccccagc
     4261 tcggcggcag gacgatcggc tgaacctaac gcgtgaacgc cccccattac agccgcgaca
     4321 ataccaaacg ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc
     4381 tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg
     4441 tgaccgctga taatcaggcg cagataatcg acgataaaat caagcatgac gctatccgcg
     4501 aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc
     4561 agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga
     4621 tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgctttcagc
     4681 gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca
     4741 ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa
     4801 ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc
     4861 ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa
     4921 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgccacaccg tatcagttaa
     4981 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
     5041 agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca
     5101 acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
     5161 agtaatccac tttcaacaaa ccgcaccaat gtcctggcac gggttaatgc cagcagtggt
     5221 ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
     5281 acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca
     5341 tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc
     5401 ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt
     5461 tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata
     5521 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
     5581 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
     5641 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
     5701 caaaacggaa gtgacaaacc agctgattgg tttctgccag cttaaccatt tgcggaag
//
LOCUS       DAECNO010000170         5738 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.173, whole genome shotgun sequence.
ACCESSION   DAECNO010000170 DAECNO010000000
VERSION     DAECNO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.173"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..242
                     /locus_tag="KKO08_003832"
     CDS             <1..242
                     /locus_tag="KKO08_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319896.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(239..1180)
                     /locus_tag="KKO08_003833"
     CDS             complement(239..1180)
                     /locus_tag="KKO08_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBD6319897.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1192..1681)
                     /locus_tag="KKO08_003834"
                     /pseudo
     CDS             complement(1192..1681)
                     /locus_tag="KKO08_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1685..2905)
                     /locus_tag="KKO08_003835"
     CDS             complement(1685..2905)
                     /locus_tag="KKO08_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBD6319898.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPCKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2909..3652)
                     /locus_tag="KKO08_003836"
     CDS             complement(2909..3652)
                     /locus_tag="KKO08_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBD6319899.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3543..5009)
                     /locus_tag="KKO08_003837"
     CDS             complement(3543..5009)
                     /locus_tag="KKO08_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBD6319900.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5009..5242)
                     /locus_tag="KKO08_003838"
     CDS             complement(5009..5242)
                     /locus_tag="KKO08_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBD6319901.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5230..5709)
                     /locus_tag="KKO08_003839"
     CDS             complement(5230..5709)
                     /locus_tag="KKO08_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319902.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1227 a         1618 c         1435 g         1458 t
ORIGIN      
        1 tctttggcat tttttacctc gttacgcgcg tctgtggctt tgccaacgga tacttccgcg
       61 tgcgcacgag tgaaccagtg cgtggcaacg tcttcctcca caacatgacg gcctttaaca
      121 aactcgcgcc gagaaccgtc gggaagcgtg agcacaaacg gggtatgtac gtgtattact
      181 gcatcatttt ttgccatcgg gtcatcctta atggccccgc cagggggcca tgtggctgtt
      241 aaatgccatc aacgtacgaa atggtttctt tgtacactgg ctcgactgca cccagcttgc
      301 cgtagtaagt gacgatctga tacagaccgc gatactgcac cggcacgctc tgaagcggaa
      361 ccagcgggta gcggacgtat tttttatcgt tggtgtacgc aaccatgcga tccttattcc
      421 ccacaccacg gcctttcagc catttaaccg cgcggatatt cagcggaaca ccgttctggt
      481 gatagctgat ggtgttggtc tgaaggttcg tcaacaggga ctggttacct gcagatgaaa
      541 cgatgatgct ggacaacaga gcaaactgct caggcgggat cagcaaatca cgcggaacca
      601 cagagtaacc agaagcggcc cacgcatcag acagcacctg gttaatgctt gcgcggattt
      661 cgtccggtgt tgaggttgcc cacgttttgg cagcgttgtt gacaggcacg ccgtccaggg
      721 taacaaggcc tttcaggttt aatgcggaat cgccaacata tacctgttca tcgttatcca
      781 tctgccattt cagttgcata ccgtcatact tctgcgtatc aatcgggcgg ccgacctgct
      841 gagcagcctg caattctatg accgtccagc caagttccat cccccacagg ttcagcgggt
      901 taccggattt gccgatatcc acgttcacgc cagcaatagc ggttgagtct ttgcctaccc
      961 agttttttcc attcggattt gcgccagtac ccgcagcggc gaagctggta ttcgtccagc
     1021 tggaaatgtc atctgcgatg gaggcgtctt cacgcagttg aatatcgcgg ctccaggtgt
     1081 accccaccag tggcaggttc agcgtctggt cgagtctctc cagctccccg atgagaaagg
     1141 caccagagct gtcaacggtt gcctgatcaa aagtaatcat tcgtctgttc cttaaatctt
     1201 ccaggaaatt tctgcattgc cgtcagcatc accggcacct gtgaattcag cgttggtcag
     1261 caccacgttt ttgccactga ctgacgtgga catgaatcca cccagaggca ctttgatgga
     1321 ttcatcagtg gagatcgcct tttttgatag tgctggcatc aaaatcagaa ccgagattaa
     1381 cggtcacgta gccacgcttc atggcgtcgc ccgggaagtt cttgcctgtt cccacctggc
     1441 gaaccatgtc cggctgcgac gtggtcggat aagggcgcac gtagatcccc ttcaccttgt
     1501 ctgcggtatc accatctgcc agcggcacga aaaaaccgtc atcatcgtat ttaccagcca
     1561 ggccataggc agcgaaggcg ttatcggatt taaggaccac cggttcgacg gttaagtcct
     1621 gcgggcgaga gacagccccg gcaatgccaa caggcatccg gtacagaaat acattattca
     1681 ttttttaccc tttacggttt gcccagaatt cagcgttttg tttgttcagg gaagcgatac
     1741 tggtcatgcc catgtttagg cgctgtgcat cgccggtggt ggcgcgggtg tttcgccctt
     1801 tggcaacctc agacacggca ttaaacgcca tgtcgaccga ttgtttcggt aatttgcgga
     1861 tatccgcatc accgactatc tggcgaacca gcgttttgtc agcagaagcc agaacctcgc
     1921 gtttgaacgc ggtcggtttc atcttacggc tcagatcgat acccggaacg ataacttcgg
     1981 cacgccaggc tgagtcacca gtaatcgtgg tttcctcttc atcgtcctcg ccgtcaccgg
     2041 tcggattatc gtcaggctta ttatcgttat cgcccgtggc atttctttcc agcttagcca
     2101 gcagggcttt cagtaatgtt ttgaggtcat catcactgtc gccggttgga tctccgccca
     2161 tctctggtgc tttgtccggt agtggttgct gcggggacag gttgatgttg agattaacgc
     2221 cctgcggcaa atccccctca tctcctgtaa ccgatgcggg agccgactcc accagttcgt
     2281 tcatggtgtc ggcatctcct gtcttgatgg ccgcacgcat gcggttccac cagtttttct
     2341 tttgatttgc cattgtgtct ctgtctccaa ttgcacaacg atttccggct ctgcctttag
     2401 ggacaagagc cacatggttt ccggtaatat cgacctgctc ggctttacat ggctcggtct
     2461 gctcgtactc cgcgtcatag ccgcacgaca cttcgcgcag gccatcttcg ataagctgaa
     2521 tggcgctttc gtctttgacg ataaggtcag ccagcatcaa atcagactgc tcacccgtcc
     2581 cgcgccggac attctggagg tgcccgacag caagcacttt ccagttctcg ggatttacca
     2641 gccgcacatt cccgttttca tcttcaggat gcaggatcgt gatgctcatc ccttcgaatg
     2701 aggcaagcgt ggccggatgg aatacctgct caggagaacg cgtgacgact atttcaacga
     2761 acttatcggg tttcagtttt ggcaggtcat cagcaccata gagctgctta cctgttcgtc
     2821 ctatcggcac gtctttgcac agcaacgagc cgtcagccag gcggtatcgg gtttccccga
     2881 gcctggtctc aaaaaagtat ttcatgggtt atgctgccct tttcattgcg aatggaacgc
     2941 gaacaaacac gatctcttta tagcagcggc agtttggcag ctcgcccgcg tggccggtca
     3001 tgccgtccaa agttggaggt gaatcccagc ggacgaattt acctttcatg tgatcgtggg
     3061 aatctcggac atcgccatca tcggctgtac gccaaatata accatccgag ccaatagcta
     3121 tcgctcgggc catatccagc gcgcccgttg ctcttcccag ttccgttcgg gcaatcagat
     3181 cggccctaga cttagcaaca tcgccggtag atgcaatctc tttagcaaac tgctccgccc
     3241 gaccaccggt gacaacagcc tctatcgctt tgttctggat gtcgtacacc ctgtctgcgg
     3301 cctcgagagg cagagatttg atgtacttga cctgttcggc gatgatggat tgcatcacct
     3361 ggcccacagg agcgctttcc acaagattgc ggagctcgcg actgatgttc ttgctgtgtt
     3421 gccgccaaac tttctcgttc tgccgggtta ggtccgcagt aaagttttcc gcgacctttg
     3481 tcgcccaggg ggtgatgatt tcactgtaac gctccagcgc cgcaataatt tccgtgatac
     3541 tgtcatttga accatcgtag cgaccattta cgatgtctcc gaccgccctc gctatcctgc
     3601 gtaggctggt tcgataccgg atttccgcct gacggctcct gcggttcgtc atcaggttcg
     3661 ccgatgccgg gcggcgcttc atcttcggca ttctcgatgt cctcgttggt aatggatgcc
     3721 cctatgccgg ttacgtcaga attttctcgc aaatcagtca tagcggcttt cagtgtcatc
     3781 agaccatcac ccagcgccgt actgattgcg ttggtggtgt ttaacgccac cgttgagcga
     3841 tcgacatcag acatttgcca gagcgggtta aactcaaacg tgaaatcgtc cggcagcggc
     3901 ttgccaagtt ccgaacgatg catgatgtcc agtatccgcc gcaccggaag acgtaaacgc
     3961 ctctcctgca acgaactgat gcgatcgtaa tagttggcaa ggtctgcatc accggtagaa
     4021 aatcctttcg gggactgtcc gaacaaccgc accagtggga taccaacagc gccactaatc
     4081 tgttctgcaa actgcgaaag gatgtcatcc agaccactga agctgtactg atgggtttca
     4141 aacttatccc gcgagtccat gagcgtcatg ccttcattac tctggaactg tcgaatcagg
     4201 tcgatattct tcagcaacgc ttcatacgca ggaccaccaa gtgcgataag ctcgcgtagc
     4261 ttctccacgc tgtaggtacg caaatgcgcc ttgtagacca gctgcgccgc gccgacagta
     4321 gcgctgtcga acgcggtaag acgatcccag atacgctcta caaccgacat tccccattcg
     4381 ttctcggtca tcttctgctg aaatggcaac gtgacgccat caaagcgaat caggcgactg
     4441 tgatgaatgc gccaggcagg aattcccgtt gctgtggtca ccacatcgta aaactcaggt
     4501 ttacccaggt ccggccccat atctttaatg cggcgggtca gtgccgggtc gaccatccag
     4561 cggtcgagcg ggagaatccc cttaaacttg cccttaccga tggtttcggg tcgcagcggg
     4621 gtcattggtg cctgcccctc aatcatgatg aaacccaccg cgccgccgta gaggcgcgac
     4681 catttcagca cgtcattcag cgcatcccag atttgcaact catccagttg tgattcgaga
     4741 atgccacgat cttttgcatc aatttccgaa gtgatgcgaa tgcctttgcg ggtcatatca
     4801 tccgggatag catcgactgc ttcgccgatg atccaggatg aacgatagga ccattccacc
     4861 agcatgcggt tacgactggt gaaattagcc cggtaggtgg atgctgagtg ctggttaggt
     4921 gtctgcatcc ctactcgggc aataaaattc tcataaccat cagctgtggc ctgcacagtt
     4981 cgccgcaggg cttgtttgtt tcgtgccatc aggcctgtct ccccagctgt tcccagatat
     5041 ccagcgatgt atcgattggc gcgaaggcca taatgaatgc atcagcaacg tttggtgacg
     5101 gtatctcgcg ttttgcgagg tcttttttac tttcgaccat cacacgtccg ttacggtcga
     5161 aatcacgatg aggtgttgtc agttccagtt tcagcttttc aagcaacgga caacgagaat
     5221 ctatgctgat cagctcatcc acaggatact gttctccgtt gttaatggcg ttaaacgtat
     5281 ttctgaaacg gtcagccacc atgcctgagc cttaagattt gcgaaaaagt ctttgttggg
     5341 gatgccgttg tactcgtcat ctggctcatg cacaccagca ccggcgttaa acctctggta
     5401 attcacacgt cgcgcgtatg cgttctcgct cttccggtca gcgttaattt cagagaattt
     5461 agcaccggca gacgcaccaa caccgataga gtcgtaaaca atatctgctt cacgctccag
     5521 cgccgcctga taagtacgct ggcagctctt cagtaattca tcttctttgg ccttccattc
     5581 gtcggcccag aaaacaacgg atccgtgacg gtaaacgtta gcgcacttat ctgtaccact
     5641 gtcagccacg tcaaagccaa tacgctttct tccactgggt tcgaaattta acgttttgtg
     5701 cgcatccact gcggcttcta tccaagacag tttgatga
//
LOCUS       DAECNO010000171         5702 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.174, whole genome shotgun sequence.
ACCESSION   DAECNO010000171 DAECNO010000000
VERSION     DAECNO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.174"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..219)
                     /locus_tag="KKO08_003840"
     CDS             complement(<1..219)
                     /locus_tag="KKO08_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBD6319903.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="KKO08_003841"
     CDS             complement(216..1124)
                     /locus_tag="KKO08_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBD6319904.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1320..2087
                     /locus_tag="KKO08_003842"
                     /pseudo
     CDS             1320..2087
                     /locus_tag="KKO08_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="KKO08_003843"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="KKO08_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="KKO08_003844"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="KKO08_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBD6319905.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="KKO08_003845"
     CDS             5533..>5702
                     /locus_tag="KKO08_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319906.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DAECNO010000172         5690 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.175, whole genome shotgun sequence.
ACCESSION   DAECNO010000172 DAECNO010000000
VERSION     DAECNO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.175"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(23..589)
                     /gene="satP"
                     /locus_tag="KKO08_003846"
     CDS             complement(23..589)
                     /gene="satP"
                     /locus_tag="KKO08_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBD6319907.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(738..1451)
                     /gene="msyB"
                     /locus_tag="KKO08_003847"
     CDS             complement(738..1451)
                     /gene="msyB"
                     /locus_tag="KKO08_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBD6319908.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1477..1881)
                     /locus_tag="KKO08_003848"
     CDS             complement(1477..1881)
                     /locus_tag="KKO08_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBD6319909.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2258..4174
                     /gene="dnaK"
                     /locus_tag="KKO08_003849"
     CDS             2258..4174
                     /gene="dnaK"
                     /locus_tag="KKO08_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBD6319910.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4263..5393
                     /gene="dnaJ"
                     /locus_tag="KKO08_003850"
     CDS             4263..5393
                     /gene="dnaJ"
                     /locus_tag="KKO08_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBD6319911.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="KKO08_003851"
     CDS             complement(5497..5649)
                     /gene="mokC"
                     /locus_tag="KKO08_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBD6319912.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1496 a         1409 c         1495 g         1290 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact
      301 gtgcatttgg cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca
      361 gccagaaaga accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact
      421 ccagcagacc agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa
      481 taccgtccag agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga
      541 agcccatcag gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa
      601 aatcatcatc gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg
      661 cggcataata atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt
      721 ttccccggtt tccggtatca gacctgagtg gcgctaacca tccggcgcag gcaggcgatt
      781 tgcagtacgg ctggaatcgt cacgcgatag gtgctgccgc tgaccgcttt aaccccattt
      841 aatgctgcgc ccacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg
      901 ctcattgctg cgtgggtgcg taaaatgcgg gtcagttggc tggaaagcaa atgcgacaca
      961 ccttttgcca ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc
     1021 ctggcatcga ccgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta
     1081 ttacgcagaa attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg
     1141 gctttcagct tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt
     1201 ccgcgcagtt tgttggcgat actatcgcca ccaaaatgct gtaattctcc ggcaatcagc
     1261 tgccagttgc ggcgatgttg ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc
     1321 atcagtatgc tggagaggcg agttttgcct ttttcattat gggtgagcaa tcgggcgaaa
     1381 tttgccaact gttcctcact acaatgctga agaaaatcca gatctgaatc attcaggtaa
     1441 ttaacattca ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag
     1501 cgtggccata tccgatgagt tcaccgtatg accggaaaag gtgatttttg agacgcagcg
     1561 tttattgtcg ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc
     1621 tgaaggaata ttcaggctct gactggcgct acgggcggct ttgaaataaa ccgatgcacc
     1681 acttaactgt aaatcgccat ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac
     1741 aggaagtttc agcgccagat cgttggtttc gttacgcggc attgcaatga cgccgaggag
     1801 tttatggtcg tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga
     1861 aatcgtaaaa acggatttca taaggattct cttagtggga agaggtaggg ggatgaatac
     1921 ccactagttt actgctgata aagagaagat tcaggcacgt aatcttttct ttttattaca
     1981 attttttgat gaacgccttg gctgcgattc attctttata tgaataaaat tgctgtcaat
     2041 tttacgtctt gtcctgccat atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca
     2101 tctccccctt gatgacgtgg gttacgaccc catttagtag tcaaccgcag tgagtgagtc
     2161 tgcaaaaaaa tgaaattggg cagttgaaac cagacgtttc gcccctatta cagactcaca
     2221 accacatgat gaccgaatat atagtggaga cgtttagatg ggtaaaataa ttggtatcga
     2281 cctgggtact accaactctt gtgtagcgat tatggatggc accattcctc gcgtgctgga
     2341 gaacgccgaa ggcgatcgca ccacgccttc tatcattgcc tatacccagg atggtgaaac
     2401 tctggttggt cagccggcta aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc
     2461 gattaaacgc ctgattggcc gccgcttcca ggacgaagaa gtacagcgtg atgtttccat
     2521 catgccgttc aaaattattg ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca
     2581 gaaaatggca ccgccgcaga tttctgctga agtgctgaaa aaaatgaaga aaaccgctga
     2641 agattacctg ggtgaaccgg taactgaagc tgttatcacc gtaccggcat actttaacga
     2701 tgctcagcgt caggcaacca aagacgcagg ccgtatcgct ggtctggaag taaaacgtat
     2761 catcaacgaa ccgaccgcag ctgcgttggc ttacggtctg gacaaaggca ctggcaaccg
     2821 tactatcgcg gtttatgacc tggggggtgg tactttcgat atttctatta tcgaaatcga
     2881 cgaagttgac ggcgaaaaaa ccttcgaagt tctggcaacc aacggtgata cccacctggg
     2941 tggtgaagac ttcgacagcc gtctgatcaa ctatctggtt gaagaattca agaaagatca
     3001 gggcattgac ctgcgcaacg atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa
     3061 agcgaaaatc gaactgtctt ccgctcagca gaccgacgtt aacctgccat acatcactgc
     3121 agacgcgacc ggtccgaaac acatgaacat caaagtgact cgtgcgaaac tggaaagcct
     3181 ggttgaagat ctggtaaacc gttccattga gccgctgaaa gttgcactgc aggatgctgg
     3241 cctgtccgta tctgatatcg acgacgttat cctcgttggt ggtcagactc gtatgccaat
     3301 ggttcagaag aaagttgctg agttctttgg taaagagccg cgtaaagacg ttaacccgga
     3361 cgaagctgta gcaatcggtg ctgctgttca gggtggtgtt ctgactggcg acgtaaaaga
     3421 cgtactgctg ctggacgtta ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat
     3481 gacgacgctg atcgcgaaaa acaccactat cccgaccaag cacagccagg tgttctctac
     3541 cgctgaagac aaccagtctg cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc
     3601 ggctgataac aaatctctgg gtcagttcaa cctagatggt atcaacccgg caccgcgcgg
     3661 catgccgcag atcgaagtta ccttcgatat cgatgctgac ggtatcctgc acgtttccgc
     3721 gaaagataaa aacagcggta aagagcagaa gatcaccatc aaggcttctt ctggtctgaa
     3781 cgaagatgaa atccagaaaa tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa
     3841 gtttgaagag ctggtacaga ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa
     3901 gcaggttgaa gaagcaggcg acaaactgcc ggctgacgac aaaactgcta tcgagtctgc
     3961 gctgactgca ctggaaactg ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat
     4021 gcaggaactg gcacaggttt cccagaaact gatggaaatc gcccagcagc aacatgccca
     4081 gcagcagact gccggtgctg atgcttctgc aaacaacgcg aaagatgacg atgttgtcga
     4141 cgctgaattt gaagaagtca aagacaaaaa ataatcgccc tataaacggg taattatact
     4201 gacacgggcg aaggggaatt tcctctccgc ccgtgcattc atctaggggc aatttaaaaa
     4261 agatggctaa gcaagattat tacgagattt taggcgtttc caaaacagcg gaagagcgtg
     4321 aaatcagaaa ggcctacaaa cgcctggcca tgaaatacca cccggaccgt aaccagggtg
     4381 acaaagaggc cgaggcgaaa tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact
     4441 cgcaaaaacg tgcggcatac gatcagtatg gtcatgctgc gtttgagcaa ggaggcatgg
     4501 gcggcggtgg ttttggcggc ggcgcagact tcagcgatat ttttggtgac gttttcggcg
     4561 atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata
     4621 acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc cgcattccga
     4681 ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca cagccgcaga
     4741 cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg ccagggattc ttcgctgtac
     4801 agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg tgcaacaaat
     4861 gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg
     4921 tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac
     4981 cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc gagcgtgaag
     5041 gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa
     5101 tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg
     5161 gtaagctatt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg
     5221 atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgaaaag cagaaacagc
     5281 tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac agcccgcgct
     5341 caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc taacctcccc
     5401 aaaagcctgc ccgtgggcag gcttgggtaa aaatagggtg cgttgaagat atgcgagcac
     5461 ctgtaaagtg gcggggatca ctccccgccg ttgctcttac tcggattcgt aagccgtgaa
     5521 aacagcaacc tccgtctggc cagttcggat gtgaacctca cagaggtctt ttctcgttac
     5581 cagcgccgcc actacggcgg tgatacagat gacgatcagg gcgacaatca tcgccttatg
     5641 ctgcttcatt gctctcttct ccttgacctt acggtcagta agaggcactc
//
LOCUS       DAECNO010000173         5683 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.176, whole genome shotgun sequence.
ACCESSION   DAECNO010000173 DAECNO010000000
VERSION     DAECNO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.176"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            872..3157
                     /gene="nrdA"
                     /locus_tag="KKO08_003852"
     CDS             872..3157
                     /gene="nrdA"
                     /locus_tag="KKO08_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBD6319913.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3391..4521
                     /gene="nrdB"
                     /locus_tag="KKO08_003853"
     CDS             3391..4521
                     /gene="nrdB"
                     /locus_tag="KKO08_003853"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBD6319914.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4521..4775
                     /gene="yfaE"
                     /locus_tag="KKO08_003854"
     CDS             4521..4775
                     /gene="yfaE"
                     /locus_tag="KKO08_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBD6319915.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KKO08_003855"
     CDS             complement(4829..5479)
                     /gene="inaA"
                     /locus_tag="KKO08_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBD6319916.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1488 c         1349 g         1390 t
ORIGIN      
        1 atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca actcgctttg
       61 atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga gaaaagagat
      121 gcaatcattg acgggagtag agataaatac tccttgcata gaaagataat ccgcatatta
      181 atctgaccat cattttatat aagacggcat atttaaacgc attatacaat atgggttatc
      241 tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt tgatgaataa
      301 tatcagtgcg tcataattca agttaataac cttaagggat accagttata tttaaactaa
      361 attaaagtaa tgaataattt tcttataata taaggtaaat taacaaaatg gcttcgcatt
      421 taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac attcgcttat
      481 atattgacca caactgatac atcagattat gtgatgactc gtgcttagat caatttttgc
      541 aatcattagc aaaaagatta ataaaccatc tatatcaatt tatccaacct attctgccgt
      601 tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg actttcccgg
      661 acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt tatccacaaa
      721 gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc aaacataccc
      781 cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg aaactattgc
      841 ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg tgacaaagcg
      901 cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg attgggcggc
      961 agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca ttcagtttta
     1021 tgacggtatc aagacctctg acatccacga aactattatc aaggctgccg cagacctgat
     1081 ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct tccacctgcg
     1141 taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg tgaaaatggt
     1201 cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag agttcaagca
     1261 gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg ccgttaagca
     1321 gctggaaggc aaatatctag tacagaaccg cgtgaccggc gaaatctacg aaagcgccca
     1381 gttcctttat attcttgttg ccgcgtgcct gttctcgaac tacccgcgtg aaacgcgcct
     1441 gcaatacgtg aagcgttttt atgacgcggt ttccacattt aaaatttcgc tgccgacgcc
     1501 aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac tgatcgagtg
     1561 cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgtgaaat atgtttccca
     1621 gcgtgccggg atcggcatca acgccggacg tattcgtgcg ctgggcagcc cgattcgcgg
     1681 tggcgaagcg ttccataccg gctgtatccc gttctacaaa catttccaga cagcggtgaa
     1741 atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc cgatgtggca
     1801 tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggggttgaag gcaaccgcgt
     1861 gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgcc tgctgaaagg
     1921 tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg cgttcttcgc
     1981 cgatcaggaa gagtttgaac gcctgtatac caaatatgag aaagacgaca gcatccgcaa
     2041 gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg cgtctaccgg
     2101 tcgtatctat attcagaacg ttgaccactg taatacccat agtccgtttg atccgaccat
     2161 cgcgccagtt cgccagtcta acctgtgcct ggaaattgcc ctgccgacca aaccgctgaa
     2221 cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt tcaacctggg
     2281 cgcaattaat agcctggatg aactggaaga gctggcgatt ctggcggttc gtgcacttga
     2341 cgcgctactg gattatcagg attacccgat cccggccgcc aaacgtggag cgatggggcg
     2401 tcgtacgctg ggtattggtg tgattaactt cgcttactat ctggcgaagc acggtaaacg
     2461 ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcga ttcagtatta
     2521 cctgctgaaa gcgtctaatg agctggctaa agagcaaggc gcgtgcccgt ggtttaacga
     2581 aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc tggatgccat
     2641 cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca aaacgcacgg
     2701 cctgcgtaac tccacgcttt ctgccctgat gccgtccgag acctcttcgc agatctctaa
     2761 cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt cgaaagacgg
     2821 tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg agctgctgtg
     2881 ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga aatttatcga
     2941 tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag gaaaagtgcc
     3001 gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca aaacgctgta
     3061 ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac cgtcaatcca
     3121 ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg gatgcggcgt
     3181 aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc cagcgtcgca
     3241 tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc tcggatttgt
     3301 aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa aatgccttat
     3361 ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc acagacgaaa
     3421 aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc tcgctacgat
     3481 cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt cttctggcgt
     3541 ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc ggagcacgaa
     3601 aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat tcagggtcgt
     3661 agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac ctgggtcgaa
     3721 acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat tcgtaatatc
     3781 gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat ccagaaacgt
     3841 gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta ctggcacctt
     3901 ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct gcgcgagctg
     3961 aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat ccgcttctac
     4021 gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg taacgccaag
     4081 attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca gcatatgctg
     4141 aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga agaatgtaag
     4201 caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg ggcggattat
     4261 ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca gtacgttgaa
     4321 tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca gacgcgctcc
     4381 aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt tgctccgcag
     4441 gaagtggaag tcagttctta tctggtcggg cagattgacg cagaagtgga caccgacgat
     4501 ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc acacaactgc
     4561 tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat gtggcggttg
     4621 agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc gcaggtcagg
     4681 tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg ccctgttgtt
     4741 gccgggcaaa aggcgatatt gaaatcgagt tgtgatcggg tactagcgcc agatacgacg
     4801 cttacgcgta ttatctggcc tacaacgatt acatggcgta gtaatacgct ttcacctgct
     4861 cccagtccgc tttagggatc ggctccagat atttttccag ctggcggaag tcatgattaa
     4921 tcgctttatc gcgacgcaag cggcgacgac ttttctccag atcaagaaaa ccagcttctg
     4981 ccttaccttc tgttttcaca tagatatggc gaacatagca acagccatgc tgacgattaa
     5041 cgctatgcat tttcttaaac gccagtgcca ctgctttcaa catggcttgc cgtacttcgt
     5101 cagaataagg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     5161 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     5221 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     5281 caatcgttgg tcggccgaac ggataacgta ctgaatgaaa cagatgatgc gtcatgcgct
     5341 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     5401 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5461 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5521 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgct
     5581 acaggtgtga caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc
     5641 actaatagtg cgcccagcag ataaaatgtg aatttaatca gtc
//
LOCUS       DAECNO010000174         5644 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.177, whole genome shotgun sequence.
ACCESSION   DAECNO010000174 DAECNO010000000
VERSION     DAECNO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.177"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(30..398)
                     /locus_tag="KKO08_003856"
                     /pseudo
     CDS             complement(30..398)
                     /locus_tag="KKO08_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            924..1898
                     /gene="hemB"
                     /locus_tag="KKO08_003857"
     CDS             924..1898
                     /gene="hemB"
                     /locus_tag="KKO08_003857"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBD6319917.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2004..2855)
                     /gene="tauD"
                     /locus_tag="KKO08_003858"
     CDS             complement(2004..2855)
                     /gene="tauD"
                     /locus_tag="KKO08_003858"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBD6319918.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2852..3679)
                     /gene="tauC"
                     /locus_tag="KKO08_003859"
     CDS             complement(2852..3679)
                     /gene="tauC"
                     /locus_tag="KKO08_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBD6319919.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3676..4443)
                     /gene="tauB"
                     /locus_tag="KKO08_003860"
     CDS             complement(3676..4443)
                     /gene="tauB"
                     /locus_tag="KKO08_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBD6319920.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4456..5382)
                     /gene="tauA"
                     /locus_tag="KKO08_003861"
     CDS             complement(4456..5382)
                     /gene="tauA"
                     /locus_tag="KKO08_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6319921.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1381 a         1528 c         1410 g         1325 t
ORIGIN      
        1 gttgtctgga gattcagggg gccagtctaa tcagttgtca tgcactcgga ataaatcata
       61 ccgtgaatag tgctgttttt gattgtcaga ctattggcgt tggtattggt agaaataccg
      121 tcgtccagat agtaggtaga aatcacgccg ttaacggtag cgttattaat gaccgggtag
      181 atatcgccgt tgtaatagct atcagcggca tagttgttcc agcctacata accatcatag
      241 taaacgtcat cagcataggt ggcgtcgttg tagtgatgga aagtattgta agtttgacca
      301 gaaatatcgt tggtcgctgc attagcctga gaggtgatag ccagagtgca agccaatgct
      361 aattgtgata ctacaagttt ctttttccag gagtgcattt gtcatccctc ctcagggacg
      421 taataagttg atccatcaat tatcaatgca tagaaaacat attatgtttc gcgctaacga
      481 ttatgctgcg ttacagacga atggttcaat tattctttcc cctaagtccg aatgtggaca
      541 tatataattt atggagaaaa acacgcatca aattgataaa aggaagatat ttcagtatgt
      601 tgcagataat gttatttttg atgcgtacgc gttataaata tcaaaaacac aaaccagata
      661 aatatcttat caatatgcaa caaagtttga attattcaat ttatagcagc acaaccaaac
      721 gccaaaaaaa ataataacca agagctataa ataaagatta aaaatatttt cagtgaatat
      781 gaaattattg ctatttctat tacaaagatg tcctggtctc tctttcattc tcttcttgtc
      841 aacatcgcga caactttcgt aaaacactcc tgccccgctt caggtatact gtgcacctta
      901 attccacaaa catcaggcag accatgacag acttaatcca acgccctcgt cgcctgcgca
      961 aatctcctgc gctgcgcgct atgtttgaag agacaacact tagccttaac gacctggtgt
     1021 tgccgatctt tgttgaagaa gaaattgacg actacaaagc cgttgaagcc atgccaggcg
     1081 tgatgcgcat tccagagaaa catctggcac gcgaaattga acgcatcgcc aacgccggta
     1141 tccgttccgt aatgactttt ggcatctctc accataccga tgaaaccggc agcgacgcct
     1201 ggcgggaaga tggactggtg gcgcgtatgt cgcgcatctg caagcagact gtgccagaaa
     1261 tgatcgtcat gtcagacact tgtttctgtg aatacacttc tcacggtcac tgcggtgtgc
     1321 tgtgcgagca tggcgtcgac aacgacgcga ctctggaaaa tttaggcaag caagccgtgg
     1381 ttgcagctgc tgcaggcgca gacttcatcg ccccttccgc cgcgatggac ggccaggtac
     1441 aggcgattcg tcaggcgctg gacgctgcag gctttaaaga tacggcgatt atgtcgtatt
     1501 cgaccaagtt cgcctcttcc ttttatggcc cgttccgtga agctgcagga agcgcattaa
     1561 aaggcgaccg caaaagctat cagatgaacc caatgaaccg tcgcgaggcg attcgtgagt
     1621 cactgctgga tgaagcccag ggcgcagact gcctgatggt taaacctgct ggagcgtacc
     1681 tcgacatcgt gcgtgagctg cgtgaacgta ctgaattgcc gattggcgcg tatcaggtga
     1741 gcggtgagta cgcgatgatt aagttcgccg cgctggcggg tgctatagat gaagagaaag
     1801 tcgtgctcga aagcttaggt tcaattaagc gtgcgggtgc ggatctgatt ttcagctact
     1861 ttgcgctgga tttggctgag aagaagattc tgcgttaatt tttttcctct cagaaagggg
     1921 gagttaacct gttgaaagta cagtgaacgc cccctcaccc taaccctctc cccaaggggg
     1981 cgagggaacc gtccgcactc gtatcacccc gcccgataaa acggtttatc cccaaggatc
     2041 gtcgcccgat gcataatccg ccgctgtggc aggtaatcgg cattggcata gtgctgagtc
     2101 acgcggttat cccaaatcgc aatatcattc ggttgccagc gccaacgcac ctgaaactcc
     2161 ggtttagtga tatgggcgaa taaaaagcct aacaaggctt cgctctcttt ctcgctgaca
     2221 tcgacaattc gcgtagtaaa gccttcattc acaaacagcg cctgtttacc gctcaccgga
     2281 tgcgttcgca ccaccggatg tagcaacgga ggatttttcg cgaccgcctc gcgccagcgt
     2341 tgatgttcct cctcggtttt gcggtatttg tattccggga atgatttacg gaaatcatgc
     2401 tccgcacgca gccagctcag cagctggcgg aagggaacag agagcgcctc ataggccgca
     2461 ataccgctgg tccagagcgt atcaccgccg gtcgaaggta actctttagc tgccagaatc
     2521 gcccctgcgg gtggcgtttc aataaatgtc acatcggtat gccagttgtc gttatccggc
     2581 ggattatcgt tatgggtatc cagcacgatg atctcgtcaa ccccttcggc atgagggtaa
     2641 acagggtgaa tatgcagttc gccaaaacgc tgggccagcg cgcgttgctg ctgcggcgta
     2701 atagcttgat cgcgcagaaa taccacctga tggcgcagca ccgcatggta aagctgttca
     2761 aactgattat cgcttaacgg gcgcgtcagg tcggcacccg aaatttgtgc gccgatatac
     2821 ggccccagcg gggtaatgct cagacgttca ctcattgtac ttctccatgc cagggcgtca
     2881 gacggcgctg taacgcgcgc agacccagtt ctaaaagaaa ggcgataatc gcaatcaccg
     2941 cgatccccgc cagtaccacg tcagtcgcga gaaattcacc cgctgactga accataaatc
     3001 ctaaaccgcg tgtcgcggca atcagctccg ccgctaccag cgtagaccag cccacaccca
     3061 gaccaatgcg taatccggtg aggatttccg gcagcgcgcc gggcaaaatg acaaaccaca
     3121 gcacctgcgc acggctggca cccagcgact gggcggcacg aatgcgaacc tgctgcgcgc
     3181 ttttcacccc tgccagcgcc gacatcgcca ccggcgcaaa aatcgctaaa tagatcagta
     3241 agatcttcga ggtttcacca ataccaaacc agatcaccat cagcggcaaa taagccagcg
     3301 gtggcaccgg acgataaagt tcgattaccg gatccagaat gccgcgcacc gtcgggctta
     3361 gccccatggc gatcccgacc ggaataccga tcaccaccgc cgcgagtagc gccagcacaa
     3421 tgcgcgtcag gctggctgcc agatgctgcc acagcgtggc gtccataaat ccctgcggtc
     3481 cggcaatggt gagcagtttt gccagtactt gttgcggcgg cggcaaaaac agcgggctaa
     3541 tcagttgcag cgccgccacc gtccaccata cagtgagtaa aaccgctaac gtgccaatgc
     3601 ttaaggtcac ctgacgcgag agcggccagc gccatttcag ccgccgcgaa tgcagttttt
     3661 cattaatgag cacactcatg agaacgcctc ccgttgctca aatacgcggc ttaaaacata
     3721 ttcgcgcatg gcgatgaatt gtggatcgga cttgatgctg cggctcgact ctcccgcaac
     3781 aaagcggcga gcaaagttga gccgcagccg ctccagcaca cggccagggc cggatgaaag
     3841 cagaaccagt tcagtcgcca taaacaccgc ttcttctata tcgtgggtaa tcaacagcac
     3901 ctgcttgccc gtctcctgcc agagtttcag cagcagagtt tgcatctggt cgcgggtgaa
     3961 ggcgtccagc gcaccaaacg gttcgtcgag taataacagc tggggattcg ccgccagcgc
     4021 gcgggcaatc cccacccgct gacgttgacc atcggaaagc tgccagatat agcgtttttc
     4081 tgcgccttcc agccccactt ttttcagcat ctggtgcgcg atttccagtc gctgcatttt
     4141 ctctatacct gccaattgca ggccgaacgc cacgttgtcc tgtacattgc gccacggtag
     4201 cagcccttca ttctgaaaaa ctacgccacg ctctgctccc ggtccctcaa tacgcttacc
     4261 cgccagttga atgctgccat gctgataagg cacaaaaccg gcaatcagat tcagcagggt
     4321 ggttttaccg cagccggacg gccccagcac caccagtagc tcgccgcttt ccagcgtcag
     4381 gttgatatct tccagtgccg gtttgccgcc ataatcggca tagagatgag agatttgcag
     4441 catctgcgcc tccttttatt gcacgaagcg cgaggtaacg tactggctgt aatcattcgc
     4501 tacagccggg accttgccct gctctttcaa aaactgcgcg gtgtcgatga ttgctttgtt
     4561 caccggtccg gtcagttctg ccgtttgttg ctgcggcgtc agataggtat ttcccttcac
     4621 cagccccgga acgtcacctt caggcacgcc gcttaaacgc gccagtttgc tgatgttttc
     4681 cggctgtttc agccacgcgt cagggttagc aatgtacggt tgctgtgcat cgatggcgct
     4741 tttggcgaac gctttcacga cctcaggatg tttctcggca aaatctttgc gcaccaccca
     4801 gacgtccagc gttggcgcac cccactgccc gacctgttca gaatcggtca gcaccttgcc
     4861 gtctttttcc agggcgttaa ccgccggtgc ccagacataa gcaccatcaa tatcaccccg
     4921 ctgccatgcg gcgataatcg cgggcggctg caggttcaca atctccacta gccctggttt
     4981 aatcccccag tgtttcagcg ccgccagcag gctgtagtgg gtggtggaga taaacggtac
     5041 ggcaatgcgt ttaccaatca aatcttcggg tttgctgata gttttcttta ccaccagcgc
     5101 ttcggagtta cccagttttg acgccagcaa gaagacttca atcggcacct gttggctggc
     5161 tgcaaccgct aatgggctgg aaccgaggtt gccgatttgc acgtcgccag aagccagcgc
     5221 ccgcacgatg ctggctccgc tgtcaaactt acgccagtcc acgctcgccc cgctctcttt
     5281 agcaaaggtg ttgtcggcct gagccacttt cgccggttcg gctgaggttt gatacgccac
     5341 ggtgacgttc accgcctgtg cctgaaaagc gatgaatgcc agtgcggcaa gaagtgtgtt
     5401 atatgactag gtaatttatt caagataacc gggaatgggt aaaaaaagac gggatttgat
     5461 gggattcaat aagttatgcg ccgattgcat tcaagataaa acgagttaaa aatgctcaat
     5521 gcttcgatat cgataaaaca ttcaagatag gcgcgccgtg tattttaatt cttttaagac
     5581 tcttcaaaaa tgtcatcgta gcctttaaaa acgaatttta aaggcaccct aaaggcaggt
     5641 caat
//
LOCUS       DAECNO010000175         5593 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.178, whole genome shotgun sequence.
ACCESSION   DAECNO010000175 DAECNO010000000
VERSION     DAECNO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.178"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            726..2234
                     /gene="ltrA"
                     /locus_tag="KKO08_003862"
     CDS             726..2234
                     /gene="ltrA"
                     /locus_tag="KKO08_003862"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBD6319922.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     NLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIAFLMRWWCKPPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2429..2533
                     /locus_tag="KKO08_003863"
                     /pseudo
     CDS             2429..2533
                     /locus_tag="KKO08_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2776..3852
                     /locus_tag="KKO08_003864"
     CDS             2776..3852
                     /locus_tag="KKO08_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc21 family transposase"
                     /protein_id="HBD6319923.1"
                     /translation="MAGKVTETAVVGGVDTHKDLHVAAVVDQNNKVLGTQFFSTTRQG
                     YRQMLAWMTSFGALKRIGVECTGTYGSGLLRYLQNAGLEVLEVTAPDRMERRKRGKSD
                     KIDAECAAHAAFSGIRTVTPKTRNGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDDKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRQINSIPITA"
     gene            3872..4177
                     /locus_tag="KKO08_003865"
                     /pseudo
     CDS             3872..4177
                     /locus_tag="KKO08_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            4233..4595
                     /locus_tag="KKO08_003866"
     CDS             4233..4595
                     /locus_tag="KKO08_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBD6319924.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYGLSTSQALANFAGFVV
                     AVSFSFYANARFTFKASTSTLRYMLYVGFMGTLSAAVGWAADRCGLPPIITLIAFSAI
                     SLVCGFFYSKFIVFREAK"
     gene            4592..5518
                     /locus_tag="KKO08_003867"
     CDS             4592..5518
                     /locus_tag="KKO08_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBD6319925.1"
                     /translation="MKISLVVPVFNEEATIPIFYKTVREFEELQQYDVEIVFINDGSK
                     DGTESIINSIASWDPLVVALSFTRNFGKEPALFAGLDHATGDAIIPIDVDLQDPIEVI
                     PHLITKWQAGADMVLAKRTDRSTDGRLKRKSAEWFYKLHNKISSPKIEENVGDFRLMS
                     RDVVENIKLMPERNLFMKGVLSWVGGKTDVVEYARAERFAGDSKFNGWKLWNLALEGI
                     TSFSTFPLRMWTYIGLFVAGISFFYGVWMIFDTFAFGNPVRGYPSLLVSILFLGGIQL
                     IGIGALGEYIGRIYVETKSRPKYILKKWKSQK"
BASE COUNT         1452 a         1286 c         1486 g         1369 t
ORIGIN      
        1 ggcagttcgc cgtgaccgag ccaaatcagg tgtggtgcgg tgatgtgacc tatagtgtgc
       61 ccggagttca gggcgggcat ggatgcttaa atgaaccgcg agtctgtctg gaatattgaa
      121 ccggtaactc acgatgagaa acccaacaat cccaccgggt gtgacggtgg agaacctgag
      181 cggcagtgac ctgcggcatg cccgcagggt gatgtaaccc gctgacaacg gggattgagg
      241 cgagatcact aagccgagat gatcctcaag gttaagtact gaaaggctga agaacatgaa
      301 cccgttaatc cgcctctgtg ggttgaaaac gtcaccacgg cctacgtgat ctgacaggcc
      361 gtgcaggagg aactggcagt gatacgtaag cactgccggt cgaaggtgtt ttgacatgta
      421 tgcgaaacac cggggcagca gcgtctatca cgcttgcgtt gctgacttct gccaacttgc
      481 ggcaagcaag gataaagagt gcgacgggca gcctcctcag tatgcctgag tccaggcagg
      541 taaaccgggg aaggtcagcg acggatgtta agggggcatg gctccgatga cgcgctggct
      601 ggcggagctt ccgtagtagt ccgcgatggg gaaagcccat tacatggcga agggaagcag
      661 tttgaatgtg tttgcgacgt gaattaactg acctaacgag gtgaagacct ttgataatca
      721 gcgaaatgca acgcaagctt gccacatggg cagccaccga tccgtcccta cggattcaac
      781 ggctgctgcg tctgataaca caaccagaat ggctggctga agcggcgcgg atcacgcttt
      841 catcaaaggg ggcccatacc cccggcgttg atggcgtgaa caaaacaatg ctacaggcca
      901 gactggctgt tgagctgcaa atcctcaggg atgaattact ctcaggccac taccagccct
      961 tgcccgccag acgggtttac atccctaaaa gcaacggcaa actgcgacca ctgggtatcc
     1021 ccgcgttgcg ggatcgtatt gttcagcggg ccatgctgat ggcgatggag ccgatatggg
     1081 agagtgattt tcatacgctc tcatatggct tccggcctga gcgcagtgtc caccacgcga
     1141 tccgcacggt gaaattacag ctcacagact gtggtgaaac ccggggacgc tgggtgattg
     1201 aaggcaacct gtccagttac ttcgacaccg tacatcatcg actgctgatg aaggctgtac
     1261 gccgcaggat cagtgacgca cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac
     1321 atatcgatgt cggtctcttt cgggcggcca gtgaaggtgt gccacagggc ggtgttatat
     1381 cgccgctatt atcgaacatc atgctgaatg agttcgatca atacctgcat gagcgctacc
     1441 tgagcgggaa agccagaaaa gatcggtggt actggaataa cagtatccaa cggggccgaa
     1501 gtacggcggt cagagaaaac tggcagtgga aacccgcggt ggcgtactgc cgctatgccg
     1561 atgattttgt cctcatcgtc aaaggcacca aagcacaggc ggaagccatc agggaggagt
     1621 gtcggggtgt gctcgaaggc agtctgaaac tcaggctgaa catggataag actaaaatca
     1681 cccatgttaa tgacggcttt atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt
     1741 atggcgagat gcgagtggtc tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat
     1801 cgctgacagc actgttatca ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac
     1861 tcaaccgaaa actgaaaggc tgggccatgt tctatcagtt cgttgatttt aaggccaaag
     1921 tcttcagtta tatcgaccgt gtcgtgttct ggaagctggc tcactggctg gcccgcaaat
     1981 accgtacagg tatcgctttc ctgatgaggt ggtggtgtaa accaccgaaa ccgggtcaga
     2041 gcaaaacgtg ggttttattt ggtaaaacca atcacggcaa gctcagcggc gaaatactgt
     2101 accggttggt ggggcaaggc aagaagctgt tccgctggcg gctacccgaa ggtaatccct
     2161 atctgaggac ggagaccaga aacacgtata catcgcgctt tacagaagtg gcaatggcgt
     2221 tcgccagcat ttaaatggag agccggatgc gctgaaaggt gcacgtccgg ttcggggagg
     2281 agaggcaggg aaatagtccg actacgccct gcctcttact ctactctaga cgggtaagcg
     2341 ctgggcgtac ctcgccgttg ttctcgacct gttcgcaaga aaaccagtgg gctgagccat
     2401 gtcgttctcg ccggacagca ggctcaccat gaaatcgctg gaaatggcat gggaaacccg
     2461 tggtaagccc ggcggggtga tgttccacag cgatagtaat aatgccggta tcagttttta
     2521 tcatcactct gtttgctgtt taaccagact ggtgtgatta ctgatgcagt gaagaccttc
     2581 ccgcatcctg actcacacag cgatcgaccc tttgtgtcct gccctggacc tgtcggttgc
     2641 cggaagcgtc ttcatgcgag gcgtctcctc accgatgcgc gtgactcaag aagggcctga
     2701 cggcttgtct cgttactgtc ctgtccgggt tatctgtctg gagattcaac tctgtttcct
     2761 cacaggagct ctgttatggc aggtaaagtt acggaaaccg ctgttgtggg tggcgtggat
     2821 acacataaag atctgcacgt tgccgctgtc gtagatcaga acaataaagt tctggggacc
     2881 cagtttttct ccacaacacg gcaaggttac cggcagatgc tggcatggat gacttcgttt
     2941 ggggcattaa agcgaattgg tgttgagtgt acaggcacct atggatcagg tctgcttcgc
     3001 tatttacaga atgccgggtt agaagttctt gaggtgactg cgccagatcg gatggagcga
     3061 cgcaaacggg gtaaaagtga caagattgat gctgaatgtg ccgctcacgc cgcattctcc
     3121 ggaataagaa ccgtcacacc caaaacgcgc aatggcatga ttgagtctct gcgggtatta
     3181 aaaacttgcc gaaaaacagc aatatcagcc cgcagagtcg ctctccagat tatccattcc
     3241 aatattatct ctgccccgga tgaattacgt gaacaactca gaaatatgac gcgcatgcag
     3301 ctcatcagga ctctgggatc ctggcggcct gatgccagtg aataccgcaa tgttaccaac
     3361 gtttatcgta tttcattaaa gtcccttgcc cgacgctatc tcgagttaca tgacgaaatc
     3421 gctgatttgg atgtcatgat tgcggcaatt gtcgatgagc tggcgcctga actgattaaa
     3481 cgtaatgcta ttggatacga aagcgcttcg cagttgctga tcacggcagg agacaatccc
     3541 caacgattaa gatcagaatc aggttttgcg gcactgtgtg gtgtcagccc tgttcccgta
     3601 tcttcaggaa aaacgaatcg ttatcgactt aaccggggtg gagatcgtgc tgcaaatagt
     3661 gcacttcaca tcattgccat cggacgtttg cgaactgacg ataaaacgaa ggaatatgtc
     3721 gccagacgag tagcggaagg gcatacaaaa atggaagcaa tacgctgcct gaagcgctat
     3781 atctcacgcg aagtttatac attactgcgt aatcaaaaca ggcagatcaa cagcatcccg
     3841 ataacggctt gactcttaga agggcgtcca tggcagtcat tatacgagca ggcagttccg
     3901 gcagttactg tggcgatacc agatcaggca gagtatgagc cggcgcggaa actgctggga
     3961 taacagccca atggaacgct tcttcagaag tctgaagaac gaatggatac cggtggtggg
     4021 ttacgtaagc ttcagcgagg cagctcacgc cataacggat tatatcgttg gatattacag
     4081 cgcactaaga ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata
     4141 ctggaaaaac tctaacgcgg tggccagttt tcgttgacca cttcactgtt gatgctaaac
     4201 tctcactttt tcagaattta ctgatttttc taatgttaaa actctttgct aagtacacat
     4261 cgattggggt tctcaataca ctcattcact gggtggtgtt tggtgtttgc atttatggat
     4321 tgtcaacaag ccaagcgtta gcgaactttg ctggcttcgt ggtggcggtg agtttcagct
     4381 tttatgccaa tgcacgtttc acatttaaag catccacctc cacgctacgt tacatgctct
     4441 atgttggctt tatgggtacc ctgagcgctg ctgtaggttg ggctgctgac aggtgcgggt
     4501 taccacccat catcacgtta atagcattct ccgcaatcag tcttgtttgc ggtttcttct
     4561 attcaaaatt cattgttttt agggaagcaa aatgaaaatt tctctggtcg ttccggtctt
     4621 caatgaggaa gccacgatac cgatctttta taaaacggta agagagtttg aagagttaca
     4681 gcaatatgat gttgaaatag tattcatcaa tgacggcagt aaagatggaa cagagtcaat
     4741 tataaattct attgcctctt gggatcctct tgtggttgca ttatcgttca cccggaattt
     4801 tgggaaggaa ccagctctgt ttgctgggct ggaccatgca accggtgatg caatcattcc
     4861 gattgatgtg gacctgcaag atcctattga agtaattcct cacctcatta ccaaatggca
     4921 ggctggggct gatatggttc ttgctaaacg aacggatcgt tcaactgacg gacgattaaa
     4981 gcgtaaatca gcggaatggt tttacaagct tcacaataaa atcagctcac caaagataga
     5041 agaaaatgtt ggcgatttca ggttaatgtc acgtgacgtt gttgaaaata ttaaactgat
     5101 gcctgaaaga aacctcttta tgaaaggcgt tctgtcttgg gttggcggta aaactgatgt
     5161 tgtagaatat gcgcgagcag aacgcttcgc tggtgattcc aagttcaatg gatggaaact
     5221 atggaacctt gcgcttgaag gcataacaag tttctcaact ttcccattac gcatgtggac
     5281 atatattggc ttgtttgtag caggtatttc attcttttat ggcgtatgga tgatttttga
     5341 tacattcgcc ttcggtaacc ctgttcgcgg atacccatca cttttggtat ctatattatt
     5401 tctaggaggg attcaactga ttggcattgg cgcccttggc gagtatatag gaagaatata
     5461 tgttgaaaca aaatccagac ctaaatacat actaaaaaaa tggaaaagtc agaaatgaat
     5521 aacaaatact taaacaaata cattacaagt gctattggct tggttgctgt ttttatttgc
     5581 ttttatatat cgt
//
LOCUS       DAECNO010000176         5580 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.179, whole genome shotgun sequence.
ACCESSION   DAECNO010000176 DAECNO010000000
VERSION     DAECNO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.179"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(1..279)
                     /locus_tag="KKO08_003868"
     CDS             complement(1..279)
                     /locus_tag="KKO08_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319926.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="KKO08_003869"
     CDS             220..405
                     /locus_tag="KKO08_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319927.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="KKO08_003870"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="KKO08_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD6319928.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="KKO08_003871"
     CDS             complement(1254..1670)
                     /locus_tag="KKO08_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319929.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="KKO08_003872"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="KKO08_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2877
                     /locus_tag="KKO08_003873"
     CDS             1837..2877
                     /locus_tag="KKO08_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6319930.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2905..2994)
                     /locus_tag="KKO08_003874"
     tRNA            complement(2905..2994)
                     /locus_tag="KKO08_003874"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2958..2960),aa:Ser,seq:cga)
     gene            3088..3885
                     /gene="mtfA"
                     /locus_tag="KKO08_003875"
     CDS             3088..3885
                     /gene="mtfA"
                     /locus_tag="KKO08_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBD6319931.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3986..4061
                     /locus_tag="KKO08_003876"
     tRNA            3986..4061
                     /locus_tag="KKO08_003876"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4019..4021,aa:Asn,seq:gtt)
     gene            4223..5485
                     /locus_tag="KKO08_003877"
                     /pseudo
     CDS             4223..5485
                     /locus_tag="KKO08_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1504 a         1322 c         1292 g         1462 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt gtgatttttg tcactgactt
     2881 aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt
     2941 tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag
     3001 atggttgtca tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt
     3061 tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca
     3121 gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg
     3181 acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg
     3241 cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta
     3301 ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta
     3361 atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat
     3421 aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg
     3481 ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct
     3541 cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt
     3601 gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc
     3661 gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ttgccagtga tcctgctgaa
     3721 tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc
     3781 ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat
     3841 cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac aactttgaag
     3901 attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa
     3961 gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4021 taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat
     4081 ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcggtgc
     4141 attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga tcaggttagt
     4201 tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca ccctcaagcc
     4261 ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg tcaagccggg
     4321 tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct
     4381 gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa
     4441 aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg gctcacgaac
     4501 accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc
     4561 gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg
     4621 gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga
     4681 ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg
     4741 ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac
     4801 cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct
     4861 tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat
     4921 gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt
     4981 cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc
     5041 cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc
     5101 cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca
     5161 taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta
     5221 cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat
     5281 ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa
     5341 taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat
     5401 gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat
     5461 tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca
     5521 ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DAECNO010000177         5560 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.180, whole genome shotgun sequence.
ACCESSION   DAECNO010000177 DAECNO010000000
VERSION     DAECNO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.180"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..642
                     /locus_tag="KKO08_003878"
     CDS             <1..642
                     /locus_tag="KKO08_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HBD6319932.1"
                     /translation="VVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAIT
                     TGKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQN
                     AVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA
                     LGLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(696..1301)
                     /gene="azoR"
                     /locus_tag="KKO08_003879"
     CDS             complement(696..1301)
                     /gene="azoR"
                     /locus_tag="KKO08_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBD6319933.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1502..5404
                     /gene="hrpA"
                     /locus_tag="KKO08_003880"
     CDS             1502..5404
                     /gene="hrpA"
                     /locus_tag="KKO08_003880"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBD6319934.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1379 a         1359 c         1540 g         1282 t
ORIGIN      
        1 gtcgtaggcg cgtgcattgt gttgatggag ctaatgatga tggttcagtt atggtggtca
       61 gtctggttat gttggccagt attatcgcta ctcattattg gccgtgggtg cggtgggctt
      121 ggcgcgataa caacagggaa agatagtcag gggaaactcc cgcccgccgt ttactggctg
      181 acattgccct ggcgcatcgg gatgtggcta tctatgcgtt ggttttgtcg tcgcctggag
      241 ccggtgagca aaatgactgc tggtgtttat ttaagggcgt ttccacgaca tattccggca
      301 cagaatgcgg ttctggacgt cacctttgaa ttccctcgtg gacgagcgac aaaagatcga
      361 ctctatttct gtgtaccgat gttggatctg gtggttccgg aagaggggga gctccgacag
      421 gccgtggcga tgctggaaac attacgcgaa gagcaaggca gcgttctggt ccattgcgca
      481 ttgggattat cgcgcagtgc gctggtagtg gcggcatggc tgttatgtta cggacactgt
      541 aaaacagttg atgaagcgat tatctttatt cgagccagac gctcgcatat tgtgcttaag
      601 gaagagcaca aagcgatgtt gaaattatgg gaaaacaggt aagtggattg agatgtggac
      661 tgaatatcta cagtccacat caagaccgtg tccggttatg cagcaacaat gctgtcgatg
      721 gctgcttttg cgtcagactg tgctttcgct gccatttccg gaccgtatgc gatcccttcg
      781 gcgaagacaa atttcacatc ggtaatgccg ataaagccga ggaacgtgga cagatacggc
      841 gtcaccaggt ccgttggtcc atctttgtgg atcccaccgc ggctggtaat aacgatggct
      901 tttttacccg ttaccagacc ttccggaccg ttctcggtat agcggaaagt aacgcctgcg
      961 cgtgcaacca ggtcaaaata gtttttcaac tgagttgaga tgttgaagtt atacatcggt
     1021 gccgcaataa cgataacgtc gtgggctttc agctcggcaa tcagctcatc ggaaagcgcc
     1081 agagcttcct gctgacgcgg agtcagcggc gcatcgctcg gacgcagagc gccaaccagt
     1141 tcgccatcca gtaccggaat cggatttgca gctaggtcgc gaacggtgat ttcatcagcg
     1201 gagtgctttt cgctccattg ttcaacaaaa taatcggaca actgattaga ctgagagtac
     1261 cctgccagga tgctggattt aagaactaat accttgctca tggtgtttcc ttatagatgt
     1321 ttgaatggac gatgccccgt tgcttgctga cactttattc acaatcctgc cacagagata
     1381 gcgcaataaa tcgaagtcta tgttcgaatt tattgaacaa cgcatagaaa gccgcgatgt
     1441 ggtactctat atctatcatt taaaagaaaa ttaatcaggc agactactgc ccactaacgt
     1501 tatgacagaa caacaaaaat tgacctttac tgccttgcaa cagcggctgg attcgctgat
     1561 gctgcgcgac agactgcgtt tttctcgccg tctgcattgt gttaagaagg ttaaaaatcc
     1621 tgatgcacaa caggccattt tccaggagat ggcgaaagag attgaccagg cggcagggaa
     1681 agtcctgctg cgtgaagcgg cacgaccgga aatcacttat cctgacaatt taccggttag
     1741 tcagaaaaaa caggacatac tcgaagcgat tcgtgatcac caggtggtga tcgtcgccgg
     1801 ggaaaccggc tccggtaaaa cgactcagct accgaaaatc tgtatggagc tggggcgcgg
     1861 tattaaaggg ctgatcggtc atacccagcc gcgtcgcctg gcggcacgaa cggtggccaa
     1921 ccgtattgcg gaagagctga aaacggagcc gggcggttgc atcggttaca aagtgcgttt
     1981 cagcgatcac gtaagtgata acacgatggt caagctgatg accgacggga tcctgctggc
     2041 ggagatccag caagaccgcc tgctgatgca gtacgacacc atcattattg acgaagcgca
     2101 cgaacgcagc ctgaatatcg attttctgct cggctatttg aaagagttgc tgccgcggcg
     2161 tcctgaccta aaaatcatta tcacttccgc gactatcgac ccggaacgct tttcgcgcca
     2221 ctttaataat gcgccgatta ttgaagtctc cggtcggacc tatccggtgg aagtgcgcta
     2281 tcgcccgatt gttgaagaag ccgatgacac cgagcgcgat cagttgcagg cgatttttga
     2341 cgccgtagac gaactaagcc aggaaagccc cggcgacatt ctgatcttta tgagcggcga
     2401 gcgggaaatt cgcgataccg ccgatgcgct gaacaagctg aacttacgcc ataccgaaat
     2461 cttgccgctt tatgcgcggc tttcgaacag cgaacagaac cgcgtgttcc agtcgcacag
     2521 cggacggcgc attgtgctgg cgaccaacgt cgcggaaacc tcgctgaccg taccggggat
     2581 taaatacgtc atcgaccccg gtacagcgcg tatcagccgc tacagctatc gcaccaaagt
     2641 gcagcgtttg ccgattgaac ccatttcgca ggcgtctgcc aatcagcgta aaggccgctg
     2701 tggtcgtgtg tccgaaggga tctgtattcg tctctattcc gaagacgatt tcctctcgcg
     2761 cccggagttt accgatccgg agattctgcg taccaacctg gcctcggtta ttttgcagat
     2821 gaccgcgctg gggctgggcg atatcgctgc gttcccgttt gtcgaagcac cggataaacg
     2881 caatatccag gatggcgtgc gtctgctcga agaactgggc gcgatcacca cggatgaaca
     2941 ggccagcgcc tataaactga cgccgctcgg tcgccagctc tcgcagttgc ctgtcgaccc
     3001 acgtctggcg cgtatggtgc tggaagcgca aaaacatggc tgcgtgcgtg aggcgatgat
     3061 tatcacgtcc gcgctctcca tccaggatcc gcgcgaacgt ccgatggaca aacagcaggc
     3121 atcggacgaa aaacatcgtc gcttccacga caaagaatcc gacttcctcg cgtttgtaaa
     3181 tctgtggaat tatcttggcg agcagcaaaa ggcgctttct tccaacgcct tccgtcgcct
     3241 gtgtcgtacc gattacctca actatctgcg cgtgcgcgaa tggcaggata tctacaccca
     3301 gttgcgccag gtggtgaaag aacttggcat tccggtcaac agcgaaccgg cggagtatcg
     3361 tgaaattcac atcgccttac tgaccggttt gctttcccat atcggcatga aagatgccga
     3421 taaacaggaa tataccggtg cacgtaacgc gcgtttctcc atcttccccg gttctggttt
     3481 attcaaaaag ccgccgaaat gggtaatggt ggcggaactg gtagaaacca gccgcctgtg
     3541 ggggcgcatt gctgcgcgta tcgacccgga atgggtagaa cccgttgctc agcatttgat
     3601 taaacgcacc tacagcgaac cgcactggga acgggcgcag ggcgcggtga tggcaacgga
     3661 aaaagtcact gtttatggtt tgccgattgt tgctgcgcgc aaggtcaact acagccagat
     3721 cgattcggcg ttatgtcgtg aactctttat tcgccacgct ctggtggaag gtgactggca
     3781 gacgcgtcac gcattcttcc gtgaaaacct gaaactgcgg gccgaagtgg aagagctgga
     3841 acacaaatca cgtcgccgcg atattctggt tgatgacgaa acgctgtttg agttctacga
     3901 ccagcgcatc agccacgatg taatctccgc tcgtcacttc gatagctggt ggaaaaaagt
     3961 cagccgcgaa acgcctgatt tgctcaactt tgaaaagagc atgttgatca aagaaggggc
     4021 ggaaaaaatc agcaagctgg attacccgaa cttctggcat cagggcaatc tcaagctgcg
     4081 tttgagctat cagtttgagc caggcgcgga cgcggacggt gtgaccgtac atattccgct
     4141 gccgttactt aaccaggttg aggaaagcgg gtttgaatgg cagatccccg gtctgcgccg
     4201 cgaattggtg attgctctga ttaaatcgtt gccgaaaccg gtacgccgta attttgtgcc
     4261 tgcaccaaac tacgccgaag cgtttttagg ccgcgtcaca ccgctggagt taccgttgct
     4321 cgacagcctt gagcgcgagt tacggcggat gaccggcgtt accgttgacc gcgaagactg
     4381 gcactgggat caggtgcccg atcacctgaa aatcaccttc cgcgtggtgg atgacaaaaa
     4441 caagaagcta aaagaagggc gctcgctaca agatctgaaa gatgcgctga aaggcaaagt
     4501 gcaggaaacg ctgtccgcgg tggcggatga cggtatcgag cagagcggct tacatatctg
     4561 gagttttggt cagctgccgg aaagttacga acagaagcgt ggcaactata aagtgaaggc
     4621 gtggccggcg ctggtggatg aacgcgacag cgtgacgatc aaactgtttg ataacccgct
     4681 ggagcaaaaa caggcaatgt ggaatggtct tcgccgtcta ctgctgctaa atatttcctc
     4741 gccgatcaag tatttacatg agaagttacc aaacaaagcc aagctggggc tgtactttaa
     4801 cccgtatggc aaagtgctgg agctgatcga cgactgtatc tcctgcggtg tggatcaact
     4861 gatcgacgcc aatggtggcc cggtctggac ggaagaaggc tttgctgcgc tgcatgaaaa
     4921 agtgcgcgcc gaactgaatg acacggtggt ggatattgcg aagcaggtcg agcaaatcct
     4981 cactgcggtg ttcaatatca acaaacgtct gaaagggcgg gtggatatga ccatggcact
     5041 ggggctttct gatattaaag cgcagatggg cgggttggta tatcgcggtt ttgtcactgg
     5101 taacggcttc aaacggctgg gcgacacgct gcgatatttg caggcgattg aaaaacgact
     5161 ggaaaaactg gcggttgatc cacatcgcga tcgtgcacag atgctgaaag tcgaaaacgt
     5221 ccagcaggcg tggcagcaat ggatcaacaa actgccgcct gcacgtcgtg aggatgaaga
     5281 cgtgaaagag atccgttgga tgatagaaga gctgcgcgtt agttacttcg ctcaacaact
     5341 tggtacgcct tatccgattt cagataagcg tattttgcag gcgatggagc agattagcgg
     5401 ttaaccctgc tatttgccta ataaatctaa aacccggtaa gcatttagcg ccgggttttt
     5461 tattaattct aaaacggtaa ggacaaaaat tcaggaattc agaaaaatac aattctcagc
     5521 tgcaagatga ataatgttta tctacagcat ttccttaaaa
//
LOCUS       DAECNO010000178         5417 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.181, whole genome shotgun sequence.
ACCESSION   DAECNO010000178 DAECNO010000000
VERSION     DAECNO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.181"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..511
                     /locus_tag="KKO08_003881"
     CDS             <1..511
                     /locus_tag="KKO08_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HBD6319935.1"
                     /translation="RGAYNEIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQ
                     TRLHLIANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDM
                     AAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGM
                     DMSHAESAH"
     gene            533..2524
                     /gene="cyoB"
                     /locus_tag="KKO08_003882"
                     /pseudo
     CDS             533..2524
                     /gene="cyoB"
                     /locus_tag="KKO08_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2514..3128
                     /locus_tag="KKO08_003883"
     CDS             2514..3128
                     /locus_tag="KKO08_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBD6319936.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3128..3457
                     /locus_tag="KKO08_003884"
     CDS             3128..3457
                     /locus_tag="KKO08_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBD6319937.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3469..4359
                     /gene="cyoE"
                     /locus_tag="KKO08_003885"
     CDS             3469..4359
                     /gene="cyoE"
                     /locus_tag="KKO08_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBD6319938.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4544..4855
                     /locus_tag="KKO08_003886"
     CDS             4544..4855
                     /locus_tag="KKO08_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBD6319939.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4839..4997
                     /locus_tag="KKO08_003887"
     CDS             4839..4997
                     /locus_tag="KKO08_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBD6319940.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4990..5388
                     /locus_tag="KKO08_003888"
     CDS             4990..5388
                     /locus_tag="KKO08_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD6319941.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1183 a         1346 c         1430 g         1458 t
ORIGIN      
        1 tcggggcgct tacaatgaaa tcgctttccc ggcgaacact ccggtgtact tcaaagtgac
       61 ctccaactcc gtgatgaact ccttcttcat tccgcgtctg ggtagccaga tttatgccat
      121 ggccggtatg cagactcgcc tgcatctgat cgccaacgaa cccggtactt atgacggtat
      181 ctccgccagc tacagcgggc cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc
      241 ggatcgcgcc gaattcgacc aatgggtcgc aaaagcgaaa cagtcgccga ataccatgtc
      301 tgacatggct gcgttcgaaa aactggccgc gcctagcgaa tacaaccagg tggaatattt
      361 ctccaacgtg aaaccagact tgtttgccga tgtgattaac aagtttatgg ctcacggtaa
      421 gagcatggac atgacccagc cagaaggtga gcacagtgca cacgaaggta tggaaggcat
      481 ggacatgagc cacgcggaat ccgcccatta aaggggttga ggaagaataa agatgttcgg
      541 aaaattatca cttgatgcag tcccgttcca tgaaactatc gtcatggtta cgatcgctgg
      601 cattattttg ggaggtctgg cgctcgttgg cctgatcact tacttcggta agtggaccta
      661 cctgtggaaa gagtggctga cctccgtcga ccataaacgc ctcggtatca tgtatatcat
      721 cgtggcgatt gtgatgttgc tgcgtggttt tgctgacgcc attatgatgc gtagccagca
      781 ggctcttgcc tcggcgggtg aagcgggctt cctgccacct caccactacg atcagatctt
      841 caccgcgcac ggcgtgatta tgatcttctt cgtggcgatg cctttcgtta tcggtctgat
      901 gaacctggtg gttccgctgc agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa
      961 cttaagcttc tggtttactg ttgttggcgt gattctggtt aacgtttctc tcggcgtggg
     1021 cgaatttgcg cagaccggct ggctggccta tccaccgcta tcgggaatag agtacagtcc
     1081 gggagtcggt gttgattact ggatatggag tctccagcta tccggtatag gtacgacgct
     1141 taccggtatc aacttcttcg ttaccattct gaagatgcgc gcaccgggca tgaccatgtt
     1201 caagatacca gtatttacct aggcatcact gtgcgcgaac gtactgatta ttgcttcctt
     1261 cccaattctg acggttaccg tcgcgttgtt gaccctggat cgctatctgg gcacccattt
     1321 ctttaccaac gatatgggtg gcaacatgat gatgtacatc aacctgattt gggcctgggg
     1381 ccacccggaa gtttacatcc tgatcctgcc tgttttcggt gtgttctccg aaattgcggc
     1441 aaccttctcg cgtaaacgtc tgtttggtta tacctcgctg gtatgggcaa ccgtctgcat
     1501 caccgtgctg tcgttcatcg tttggctgca ccacttcttt acgatgggtg cgggcgcgaa
     1561 cgtaaacgcc ttctttggta tcaccacaat gattatcgcc atcccgaccg gggtgaagat
     1621 cttcaactgg ctgttcacca tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg
     1681 gactatcggt tttatcgtca ccttctcggt gggcggtatg actggcgtgc tgctggcagt
     1741 accgggcgcg gacttcgttc tgcataacag cctgttcctg attgcgcact tccataacgt
     1801 gatcatcggc ggcgtggtct tcggctgctt cgcagggatg acctactggt ggcctaaagc
     1861 gttcggtttc aaactgaacg aaacctgggg taaacgcgcg ttctggttct ggatcatcgg
     1921 cttcttcgtt gcctttatgc cgctgtatgc gctgggcttc atgggcatga cccgtcgttt
     1981 gagccagcag attgacccgc agttccacac catgctgatg attgcagcca gcggtgcagt
     2041 actgattgcg ctgggtattc tctgcctcgt tattcagatg tacgtttcta ttcgcgaccg
     2101 cgaccagaac cgtgacctga ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac
     2161 ctcttccccg cctccgttct ataactttgc cgtagtgccg cacgttcacg aacgtgatgc
     2221 attctgggaa atgaaagaga aaggcgaagc gtataaaaag cctgaccact atgaagaaat
     2281 tcatatgccg aaaaacagcg gtgcaggtat cgtcattgca gctttctcca ccatcttcgg
     2341 tttcgccatg atctggcata tctggtggct ggcgattgtt ggcttcgcag gcatgatcat
     2401 cacctggatc gtgaaaagct tcgacgagga cgtggattac tacgtgccgg tggcagaaat
     2461 cgaaaaactg gaaaaccagc atttcgatga gattactaag gcagggctga aaaatggcaa
     2521 ctgatacttt gacgcacgcg actgcccacg cgcacgaaca cgggcaccac gatgcaggcg
     2581 gaaccaaaat cttcggattt tggatctacc tgatgagcga ctgcattctg ttctcaatct
     2641 tgtttgctac ctatgccgtt ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca
     2701 ttttcgaact gccgttcgtt ctggttgaaa ctttcttgct gttgttcagc tccatcacct
     2761 acggcatggc ggctatcgcc atgtacaaaa acaacaaaag ccaggttatc tcctggctgg
     2821 cgttgacctg gttgtttggt gccggattta tcgggatgga aatctatgaa ttccatcacc
     2881 tgattgttaa cggcatgggg ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg
     2941 tcggcacgca cggtctgcac gtcacttctg gtcttatctg gatggcggtg ctgatggtgc
     3001 aaatcgcccg tcgcggcctg accagcacta accgtacccg catcatgtgc ctgagcctgt
     3061 tctggcactt cctggatgtg gtttggatct gtgtgttcac tgttgtttat ctgatggggg
     3121 cgatgtaatg agtcattcta ccgatcacag cggcgcgtcc catggcagcg taaaaaccta
     3181 catgacaggc tttatcctgt cgatcattct gacggtgatt ccgttctgga tggtgatgac
     3241 aggggctgcc tctccggccg taattctggg aacaatcctg gcaatggcag tggtacaggt
     3301 tctggtgcat ctggtgtgct tcctgcacat gaataccaaa tcagatgaag gctggaacat
     3361 gacggcgttt gtcttcaccg tgctaatcat cgctatcctg gttgtaggct ccatctggat
     3421 tatgtggaac ctcaactaca acatgatgat gcactaagag cggcggttat gatgtttaag
     3481 caatacctgc aagtaacgaa accaggcatc atctttggca acctgatctc ggtgattggg
     3541 ggattcctgc tggcctcaaa gggcagcatt gattatcccc tgtttatcta cacgctggtt
     3601 ggggtgtcac tggttgtgac gtcgggttgt gtgtttaaca actacatcga cagggatatc
     3661 gacagaaaga tggaaaggac gaagaatcgg gtgctggtta aaggcctgat ctctcctgct
     3721 gtctcgctgg tgtacgccac cttgctgggt attgctggct ttatgctgct gtggtttggc
     3781 gcgaatccgc tggcctgctg gctgggggtg atgggctttg tggtttatgt cggcgtctat
     3841 agcctgtaca tgaaacgcca ctcagtctac ggcacattga ttggttcgct ctccggcgct
     3901 gcgccgtcgg tgattggcta ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc
     3961 ctgctggcta tcttcagcct gtggcagatg cctcactcct atgccatcgc cattttccgc
     4021 tttaaggatt accaggcggc aaacattccg gtattgccag tggtaaaagg catttcggtg
     4081 gcgaagaatc acatcacgct gtatatcatc gcctttgccg ttgccacgct gatgctctct
     4141 cttggcggtt acgctgggta taaatatctg gtggtcgccg cggcggttag cgtctggtgg
     4201 ttaggtatgg ctctgcgcgg ttataaagtt gctgatgaca gaatctgggc gcgcaagctg
     4261 ttcggcttct ctatcatcgc catcactgcc ctctcggtga tgatgtccgt tgattttatg
     4321 gtaccggact cgcatacgct gctggctgct gtgtggtaac aaaacctctc tattaaaaag
     4381 gtgctacggc acctttttta ttgtctgaga tcagagatac ggctaataac cgtatctctt
     4441 gacagagtga gcagatgcac ataaattaat caaacggttg atagccgtat tatgtatgag
     4501 atcgactccc tacgatttca ttcagttaat caaagaagtc ataatgcctg gaaaaacagc
     4561 cgctctatac gatgtcgata aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc
     4621 cgacgccaaa aacaaacttc gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt
     4681 tattttgagc gctgctatgg aacgcgccga aagtgtgctt gataaccaac gtcgccgtga
     4741 gctttcgaat caaagctggg aactgatgaa ccaactcatc gctgaacctg ctcaaccgac
     4801 gctcgccctc aaggcgttaa tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga
     4861 ttactctgcc catagtcctc tcctgtaatt atcagtctga tattacttat cctgggcaaa
     4921 aacagtttga ttgcggtaac cctgttatcg ataaatttgt acgcgctacg cgcatcgcta
     4981 aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac ttattgacag acaaagtggt
     5041 gaactgattg gcatctgtac ttttacggca tattcgctgg aaagacaacg cgtttctggc
     5101 gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca gattagtcat gttgggggga
     5161 gcgcggaagt atcaaaagcg gggctttggt caggacctac tatgtgattt ttttgaacat
     5221 gtaaaaaaaa ttcacccggc attaccaatt aaaggggttt atcttgatgc tgaccctgcc
     5281 gccattaact tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt
     5341 ggtgctgtac ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc
     5401 acccgccagg gcaagtt
//
LOCUS       DAECNO010000179         5350 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.182, whole genome shotgun sequence.
ACCESSION   DAECNO010000179 DAECNO010000000
VERSION     DAECNO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.182"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(75..557)
                     /locus_tag="KKO08_003889"
     CDS             complement(75..557)
                     /locus_tag="KKO08_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319942.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="KKO08_003890"
     CDS             complement(625..2574)
                     /locus_tag="KKO08_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBD6319943.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="KKO08_003891"
     CDS             complement(2659..2994)
                     /locus_tag="KKO08_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBD6319944.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="KKO08_003892"
     CDS             complement(2994..3350)
                     /locus_tag="KKO08_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBD6319945.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="KKO08_003893"
     CDS             complement(3350..4846)
                     /locus_tag="KKO08_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBD6319946.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="KKO08_003894"
     CDS             complement(4843..5004)
                     /locus_tag="KKO08_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HBD6319947.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="KKO08_003895"
     CDS             complement(5014..>5350)
                     /locus_tag="KKO08_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319948.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAECNO010000180         5339 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.183, whole genome shotgun sequence.
ACCESSION   DAECNO010000180 DAECNO010000000
VERSION     DAECNO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.183"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="KKO08_003896"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="KKO08_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBD6319949.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="KKO08_003897"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="KKO08_003897"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBD6319950.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="KKO08_003898"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="KKO08_003898"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBD6319951.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="KKO08_003899"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="KKO08_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBD6319952.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1456 g         1283 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtt cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcggg
//
LOCUS       DAECNO010000181         5164 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.184, whole genome shotgun sequence.
ACCESSION   DAECNO010000181 DAECNO010000000
VERSION     DAECNO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.184"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..221
                     /locus_tag="KKO08_003900"
     CDS             <1..221
                     /locus_tag="KKO08_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6319953.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(393..722)
                     /locus_tag="KKO08_003901"
     CDS             complement(393..722)
                     /locus_tag="KKO08_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBD6319954.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(727..912)
                     /locus_tag="KKO08_003902"
     CDS             complement(727..912)
                     /locus_tag="KKO08_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBD6319955.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(909..3548)
                     /locus_tag="KKO08_003903"
     CDS             complement(909..3548)
                     /locus_tag="KKO08_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBD6319956.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3756..4745
                     /gene="ldhA"
                     /locus_tag="KKO08_003904"
     CDS             3756..4745
                     /gene="ldhA"
                     /locus_tag="KKO08_003904"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBD6319957.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4856..>5164
                     /gene="hslJ"
                     /locus_tag="KKO08_003905"
     CDS             4856..>5164
                     /gene="hslJ"
                     /locus_tag="KKO08_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBD6319958.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1305 a         1323 c         1205 g         1331 t
ORIGIN      
        1 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
       61 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
      121 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
      181 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgcatgta
      241 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
      301 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
      361 tgtagcgtta tcccgatatt gcggttttgc tattaaaact ttcgcaccca tttaccgttt
      421 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
      481 gcgatatcat ctacagacac attattttgc tttgcccgtt gttggtaact cgctttacgt
      541 tcggcattaa tttcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
      601 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
      661 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
      721 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
      781 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttaggtgc
      841 agcgacttca atgcgaggcg tacagccaac cagcataaaa gatgacgtca acgcagccag
      901 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
      961 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
     1021 gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt accatcaacg
     1081 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
     1141 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
     1201 atattgtctt tcaccaccgc atctgcggta tctttatcca gccgcaaggt cattggcccg
     1261 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
     1321 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
     1381 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1441 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
     1501 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1561 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1621 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1681 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1741 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1801 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
     1861 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1921 ttaaagagga aataggtcgg attgcgccca tcgacgctaa attttaaggt tgatggcggt
     1981 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     2041 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     2101 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     2161 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     2221 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     2281 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2341 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2401 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2461 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
     2521 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagttg actgttatcc
     2581 atgcttaatt ttcctgggcc aaaattaagc accgcgttgc ccttacccgc ttgcccctgg
     2641 gtcagcacat tcagtcgtcc gctgaccaga gcgttctcaa gccctgcctg ccagttgtcg
     2701 actttgacac ccagtcggcc actcaaagga aaacctgcat acagccagct ccagcgacca
     2761 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2821 ttatcccgtg ccagcacgat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2881 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
     2941 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     3001 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     3061 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     3121 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
     3181 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     3241 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3301 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3361 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3421 cgtgtcccga gcggtagcca gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc
     3481 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3541 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3601 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3661 tttttcaata tcgccatggc tttcaattat atttgaaatt ttgtaaaata tttttagtag
     3721 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3781 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3841 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3901 gtattttcgt aaacgatgac ggcagccacc cggtgctgga agagctgaaa aagcacggcg
     3961 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag
     4021 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     4081 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     4141 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
     4201 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     4261 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4321 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4381 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4441 tcgtcaatac cagtcgcggt gcattgattg attctcaggc ggcaattgaa gcgctgaaaa
     4501 atcagaaaat tggttcgttg ggtatggacg tctatgagaa cgaacgcgat ctgttctttg
     4561 aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct gcctgccaca
     4621 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4681 agactacgct gcaaaactta agcaatctgg aaaaaggtga aacctgcccg aacgaactgg
     4741 tttaatcttg cagctcccct gcaatccagg ggagctgatt cagataatcc ccaatgacct
     4801 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa ccacaatgaa
     4861 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4921 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4981 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     5041 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     5101 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     5161 taac
//
LOCUS       DAECNO010000182         5156 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.185, whole genome shotgun sequence.
ACCESSION   DAECNO010000182 DAECNO010000000
VERSION     DAECNO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.185"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            188..445
                     /locus_tag="KKO08_003906"
                     /pseudo
     CDS             188..445
                     /locus_tag="KKO08_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KKO08_003907"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="KKO08_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBD6319959.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KKO08_003908"
     CDS             complement(1597..2349)
                     /gene="fnr"
                     /locus_tag="KKO08_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBD6319960.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KKO08_003909"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="KKO08_003909"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBD6319961.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="KKO08_003910"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="KKO08_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3603..4418)
                     /locus_tag="KKO08_003911"
     CDS             complement(3603..4418)
                     /locus_tag="KKO08_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6319962.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5156
                     /locus_tag="KKO08_003912"
     CDS             4515..>5156
                     /locus_tag="KKO08_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBD6319963.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVH"
BASE COUNT         1376 a         1175 c         1226 g         1379 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccctt gttcac
//
LOCUS       DAECNO010000183         5025 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.186, whole genome shotgun sequence.
ACCESSION   DAECNO010000183 DAECNO010000000
VERSION     DAECNO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.186"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            49..123
                     /gene="tnaC"
                     /locus_tag="KKO08_003913"
     CDS             49..123
                     /gene="tnaC"
                     /locus_tag="KKO08_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBD6319964.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            344..1759
                     /gene="tnaA"
                     /locus_tag="KKO08_003914"
     CDS             344..1759
                     /gene="tnaA"
                     /locus_tag="KKO08_003914"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBD6319965.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1850..3097
                     /gene="tnaB"
                     /locus_tag="KKO08_003915"
     CDS             1850..3097
                     /gene="tnaB"
                     /locus_tag="KKO08_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBD6319966.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3229..4404
                     /gene="mdtL"
                     /locus_tag="KKO08_003916"
     CDS             3229..4404
                     /gene="mdtL"
                     /locus_tag="KKO08_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBD6319967.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4379..>5025
                     /gene="yidZ"
                     /locus_tag="KKO08_003917"
     CDS             4379..>5025
                     /gene="yidZ"
                     /locus_tag="KKO08_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBD6319968.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1209 c         1255 g         1416 t
ORIGIN      
        1 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
       61 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
      121 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
      181 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
      241 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
      301 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
      361 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
      421 tcgtaaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
      481 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
      541 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
      601 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
      661 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
      721 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
      781 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
      841 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
      901 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
      961 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
     1021 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
     1081 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
     1141 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
     1201 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
     1261 atatggcggc ctggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
     1321 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
     1381 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
     1441 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
     1501 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
     1561 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
     1621 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
     1681 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
     1741 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
     1801 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
     1861 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
     1921 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggggtgcctt
     1981 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
     2041 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
     2101 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
     2161 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
     2221 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
     2281 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
     2341 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
     2401 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
     2461 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
     2521 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
     2581 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
     2641 cggcaacatt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
     2701 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
     2761 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
     2821 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
     2881 ctttatttac gggatcggcg gtgccgggct gtgcgccacc atctgggcgg tcattattcc
     2941 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
     3001 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
     3061 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
     3121 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
     3181 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
     3241 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
     3301 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
     3361 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
     3421 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
     3481 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
     3541 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
     3601 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
     3661 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
     3721 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
     3781 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
     3841 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
     3901 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
     3961 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
     4021 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
     4081 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
     4141 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
     4201 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
     4261 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
     4321 agcatagtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
     4381 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
     4441 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
     4501 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
     4561 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
     4621 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
     4681 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
     4741 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
     4801 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
     4861 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccctg
     4921 cgtctggtta cgcaaagatc atccggcact gcatgaaacg tggaatctgg acaccttttt
     4981 acgttatccg catatcagca tttgctggga acagagcgac acctg
//
LOCUS       DAECNO010000184         5007 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.187, whole genome shotgun sequence.
ACCESSION   DAECNO010000184 DAECNO010000000
VERSION     DAECNO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.187"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..249
                     /locus_tag="KKO08_003918"
     CDS             <1..249
                     /locus_tag="KKO08_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD6319969.1"
                     /translation="SLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAA
                     MVQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(450..1307)
                     /gene="sitD"
                     /locus_tag="KKO08_003919"
     CDS             complement(450..1307)
                     /gene="sitD"
                     /locus_tag="KKO08_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBD6319970.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1304..2161)
                     /gene="sitC"
                     /locus_tag="KKO08_003920"
     CDS             complement(1304..2161)
                     /gene="sitC"
                     /locus_tag="KKO08_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBD6319971.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2158..2985)
                     /gene="sitB"
                     /locus_tag="KKO08_003921"
     CDS             complement(2158..2985)
                     /gene="sitB"
                     /locus_tag="KKO08_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBD6319972.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2985..3899)
                     /gene="sitA"
                     /locus_tag="KKO08_003922"
     CDS             complement(2985..3899)
                     /gene="sitA"
                     /locus_tag="KKO08_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBD6319973.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4372..>5007)
                     /locus_tag="KKO08_003923"
     CDS             complement(4372..>5007)
                     /locus_tag="KKO08_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6319974.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1333 a         1222 c         1199 g         1253 t
ORIGIN      
        1 tcactgggcc gcctgtcccc ggtagtcatg ctggcaaaat ctgtcacagc acaaacacat
       61 atggcgtgga gcgatgccga taatcagcag gtgaaactgt cgatgccgga actggaagaa
      121 ctggcggcag caatggtgca ggcgcaggtc gatcgcaacg atgagattta tcgccgtcag
      181 cgggagctga aggaagagct gaatagtctg aaggatttga attcggttag gaattttatc
      241 gtggaataac agaagctgcg gcacgtcgta tgcaggaacg tgccgcggtt ggctggtaaa
      301 ctttcgatag tgcgagtatt gaatgatttc taggcgttat cgattttacg tattttttgc
      361 atgatagaat tcgtacctcc tcccaacgac cttccatggc tttccggact tctgtaggct
      421 ggcggacgat gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg
      481 gcgtttccct ttgtgcgtgc gttgatgctg gtaacggtaa acgtcatgat gaacacgacc
      541 gcgaataaga cgacaatcgt cggagctggt gcgctgtcga taaaaaagga cagccagaca
      601 ccagtcattg acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt
      661 tgtgtcagga gtactgcaat ggcgccaggc gcaatgagca gagaaataga gagaatgata
      721 cctactgctt tcagcgtcgc cacaatggtc agggaaacca tgcacagcag cccataatgc
      781 agccatctcg tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa
      841 aaatcccgcc attttacgct aataacaagt gtaaccagcc cggcaataat caccgtctgg
      901 attatatcgc cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg
      961 tctggtttaa ttgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc
     1021 cccataactg tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgcg
     1081 cagaatactc cggcgacgaa tgcccctgta gccagaggta accccagaat ccaggtaagc
     1141 acgatgccag gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg
     1201 accagaaaga ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg
     1261 tgactcataa aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca
     1321 gcgctttatg cgcacgccga cgatttgcca aaagtccatg ggtgggcgca aatacaaatg
     1381 ctaaaagaaa cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa
     1441 agtaactgac ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaattatga
     1501 gcaggcgggg aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac
     1561 agatcaccag aaacgcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg
     1621 tgaagaagag gatttttaat ctgcccggat gtaatccaat agcacgggcg tgattctcgt
     1681 cgaaaaaggt caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc
     1741 caatgatagt gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga
     1801 tggtctgaat gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa
     1861 aggacgagaa gatcaggccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa
     1921 acagcatgct gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc
     1981 ccagcatata tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca
     2041 gcgaccagcc ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccattg
     2101 ccgagaccca catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca
     2161 tcttgcttcc ctctgcaccg ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt
     2221 aataatgctt tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag
     2281 ctccaggttc tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt
     2341 gaccatgacc gtataatcgc aaaatgtcgt gactgagccg agattgtgcg ttgagacaag
     2401 catcgttttg ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt
     2461 cacatcaacg ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaattgc
     2521 tctcgccaga aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gacgatggcg
     2581 aaaatccacc atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt
     2641 ggctatacgc aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag
     2701 tacaggaaat gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg
     2761 tagcgcctgt cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa
     2821 tcccataatc gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc
     2881 aatagatcca cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca
     2941 ggtgacagta acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc
     3001 ccggctttaa ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgta
     3061 ggtaccgggc cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg
     3121 ccggtttcac gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact
     3181 gccgggatat gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct
     3241 tgttgatcgg cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga
     3301 taagaaaaag ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc
     3361 tgcttacgca ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattacgt
     3421 tggtaggttt gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg
     3481 taaatcagag cattatctgg cgacatccat gcatggggat taggtttgcc ctcatagggt
     3541 ccttcggtga tccctactgg cgtcacaccc gaagagacaa ttacttctgg aaccccattg
     3601 agatgctggt aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt
     3661 tgtgccccct gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca
     3721 ccaggcttgg ttatggatga gacttctgca gcatctccag ccacgttttt ggccatatcc
     3781 gcgatgatgg taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg
     3841 atcgagcagg tgagggcgag gcaccccaag agcatggtta ctttttttat tgagtgcata
     3901 atttagtacc tatagtgagc aaaattagaa caaacatagc aacggctatg ttttatagca
     3961 taacctatac aaaatgaaaa taattatcat taacagcttt gaggcctaaa acagggatta
     4021 ggttgtttcc tgctggcgac atgatattga ggaaaaaagt agagcagcta ttaggctttg
     4081 aaatgacttt atgaggaaaa ttgttgatgg caaaaatggg gcaaaacgct gtataagggg
     4141 caaaaaaggg gcaaaaaagg ggcaaaaaag agtgggttat cgtagcttat tgttgtcgct
     4201 gataatgttt aacatattga aaaataaata aaatacttat gagtcagata gttgtgattt
     4261 ttgcccttac ttgttcaggt cgtattgttc tttctttgta atttgttgat tttcttgcat
     4321 tatttcagtt ctctggtact aaatggggca aattgggggc aaactttgca actacgataa
     4381 ccgcgcattc aacatggcta tctgttcgtc gttcatgtca tcaatccaca taccgtaaat
     4441 ttcatacacc atttgcgcag tttcatgccc catctggctg gctataaatg ccgggttcgc
     4501 tcctgccgtc aacagccagc aggcaaaagt atgtcgcgtg tggtacggat tacggcggcg
     4561 aataccagca cgttttactg ctgcattcca tcttgtaccc aaactgctta ccgagtaata
     4621 aggtttctgt ttttcgttac acaccctggg catgaaaaca aaatgcagtt tttgtttttc
     4681 agttctgccg tactcccgat gataaaaagt gatttcgctt ttgcgatgat gcccggtcag
     4741 tttgtattgc tccttcagtg cttcaagagc cggctgcagt aatgttaccg tccggatccc
     4801 ggcatttgtt tttgggggac cgaacatatc aagtatcgtc aggtttcttc tgacattcac
     4861 aattcccttc tcgaaatcca catcctccca cgccagagct gccagttccc cgtgacgaag
     4921 cccggagtaa acggcaaatt tccacaagtt ttggctctgt cctttttcac tttccattaa
     4981 tgcattgaat tctgttttag ataacgg
//
LOCUS       DAECNO010000185         4973 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.188, whole genome shotgun sequence.
ACCESSION   DAECNO010000185 DAECNO010000000
VERSION     DAECNO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.188"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="KKO08_003924"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="KKO08_003924"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBD6319975.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="KKO08_003925"
     CDS             1020..1703
                     /locus_tag="KKO08_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBD6319976.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="KKO08_003926"
     CDS             1700..2161
                     /locus_tag="KKO08_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBD6319977.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="KKO08_003927"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="KKO08_003927"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBD6319978.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="KKO08_003928"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="KKO08_003928"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBD6319979.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEELL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KKO08_003929"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="KKO08_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBD6319980.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KKO08_003930"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="KKO08_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBD6319981.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1175 a         1303 c         1241 g         1254 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt atccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcggcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agagttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAECNO010000186         4871 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.190, whole genome shotgun sequence.
ACCESSION   DAECNO010000186 DAECNO010000000
VERSION     DAECNO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.190"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..527
                     /locus_tag="KKO08_003931"
     CDS             <1..527
                     /locus_tag="KKO08_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBD6319982.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="KKO08_003932"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="KKO08_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBD6319983.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3206)
                     /gene="gap"
                     /locus_tag="KKO08_003933"
     CDS             complement(2205..3206)
                     /gene="gap"
                     /locus_tag="KKO08_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBD6319984.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="KKO08_003934"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="KKO08_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBD6319985.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="KKO08_003935"
     CDS             4170..4343
                     /locus_tag="KKO08_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319986.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="KKO08_003936"
     CDS             complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="KKO08_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBD6319987.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4665..4805)
                     /locus_tag="KKO08_003937"
                     /pseudo
     CDS             complement(4665..4805)
                     /locus_tag="KKO08_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1120 c         1208 g         1296 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
     4501 gtgtgaatgc cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgcgct
     4561 tcatagcctt tctccttgcc ggatggcggg taagaggcta acaatctgaa ttgcttggtt
     4621 cattcgttgg cctcggtttg tactgacccc aaaaagttgg acagttaaac acgaggcata
     4681 taggtctgat tccgatattc atttggagtc agacctttta atttcaggct aattcttctg
     4741 ctgttgtagt attcaatata ttccgtaaca gcatccttca gttcgcttat attactgaac
     4801 tcattagact ggccccctga atctccagac aaccagtatc acttatttaa gtgatagtct
     4861 taatactagt t
//
LOCUS       DAECNO010000187         4867 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.191, whole genome shotgun sequence.
ACCESSION   DAECNO010000187 DAECNO010000000
VERSION     DAECNO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.191"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..970
                     /locus_tag="KKO08_003938"
     CDS             <1..970
                     /locus_tag="KKO08_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBD6319988.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1551..2774
                     /locus_tag="KKO08_003939"
     CDS             1551..2774
                     /locus_tag="KKO08_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319989.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3021..3587
                     /gene="slp"
                     /locus_tag="KKO08_003940"
     CDS             3021..3587
                     /gene="slp"
                     /locus_tag="KKO08_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBD6319990.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3743..4273
                     /locus_tag="KKO08_003941"
     CDS             3743..4273
                     /locus_tag="KKO08_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6319991.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4315..4867)
                     /locus_tag="KKO08_003942"
                     /pseudo
     CDS             complement(4315..4867)
                     /locus_tag="KKO08_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1556 a          999 c          905 g         1407 t
ORIGIN      
        1 cgcagagatg tttgtgtttc tcgccgtcga gctttcgttg ctgtttatag tgattagtgc
       61 cggtgtcagc ctgataagac aaaaggtgcc agaccataaa atccagcaga tgatgggggc
      121 cagaaaaggg agaggctatc tcctggctgc tctgttggga gccgttaccc cgttctgtag
      181 ttgctcgaca atacccatgt tacgtggatt gttgtcagcg aaagccgggt ttggtccgac
      241 tctcactttt ttatttgttt caccattact taatcccatt atcgtcggac taatgtggat
      301 gacctttggt tggaaagtca ccttgttgta cgcgattatc gcagccggcg tctccgttct
      361 tgccagtatt atcctggatt ctctggggtt tgaacgtcat atcattgcca gtaaaagctc
      421 atcagcaaat tgttgtgctc cagcaaaaac ttcgccggga acgacatata cgcccataaa
      481 agtgagttgt tgtagtccag cggctaaagc cattgagaac cccgtagtta cctgttgcag
      541 taccaaagct gtagtaagta ttaatcccat aaaactagcc accaaagatg cgttgcaaca
      601 gtttaaagat gtactgccat atcttttgtt aggagtatta ataggatctt ttatttatgg
      661 ctttattccc tcagcgtgga ttgccgctca tgcaggagca gataatccct tcgccatccc
      721 attaagcgcc gttgtcggta ttccgctgta tatccgggct gaggcggtta tccctctggc
      781 atctgttctg atgacaaaag gaatgggtct gggagcatta atggcattaa tcatcggcag
      841 tgccggcgca agcctgacgg aagtgatatt acttaaatca atgttcagaa tgccgatgat
      901 agctgcattc ctgacggtta tattaggtat ggctatcttg atgggctatt tgactcaatt
      961 gctattttaa atagatgcca gcagtaaact ataagatgca tagttagtgc catatccttc
     1021 taagtagaaa atcatagaca ggataatcct taaaaatcac aaatgattta actaatgcat
     1081 tgagaatgat gacggctcgg tcaacaagtc atcaacagac aataaccacg taatcatgct
     1141 tctctgacat tttatcggag aagcaatgac tctttgtctc agtaccctcc tcctttttaa
     1201 aaggcagatt tcagtcgcta atacaacaca tttttaaata cactttaacc tggttcatta
     1261 ttacacatct aaaacatcac aaaaatatta cttcacacac tatcggagaa cataataact
     1321 ttttaaaatg agcgtttttt gatagttgtt aacgttttcc tttacgatac tatcactgcc
     1381 ctttttttat acacaaatat taccgggtgg aaatgaaagg gaatttaaag gtaattatat
     1441 caggaagata taacgcatta tatttattgt gtgtagaaga aaatccattc ctgttctaat
     1501 tatccaattt aaaaacatta gcataaaaca gatgatgaat aaggaatgtt atgtcaattg
     1561 actttacccc aggtataata aatacatatc acggcgatat ttataactgc acaacaaata
     1621 ccgataatgt taaaacgcca gacacaccga agtggccttg tgataattgg gaagaacaac
     1681 aacccattaa ttccacattc tctggagaag gatacaaccc tgaacaattt gatttagcgc
     1741 aacaacaact ccaacaaatt aatgcatgcc acacgaacac cacatataca aatgcagact
     1801 actcaaaagt tgtggttcaa cttgtaagtc ttattaataa cattgaaacg ataagttcga
     1861 caccacttac gcaacagaca caatcaatac ttaatcagat tcataacatt agatacgaaa
     1921 aggataagga ttctgtatgt caaatcattg ttgtcgttaa tccagaaccc aacaatccaa
     1981 tcataactaa aattctcgta gaagaaggaa tacccgaaag attcagtgtc caaaccgttt
     2041 cttccgataa taaaaatttt gctgggcaac gagctgactt accaactgat ataagagaca
     2101 ttcaaagctt gtacttaaaa atggcgaagc tgtatattga gcacagtaaa aatgaaaata
     2161 ggatggaagc ccttgctgga tgcgacttta ttgatttcaa catgacaggg caagatatgt
     2221 ccaagctagt cctaacctta tcaaaatttt attttgaaga tctacttaat ataaatttca
     2281 ctgacgcaaa tttatttaac actatttttt cacacaagga aaatcccatc cccaaactac
     2341 ataaatatga acaacatctt gacaaacaaa tcaatggttt gttttctaca ttgttaacta
     2401 ttaatgacaa ctctttacgc gcgaaagcgg aaattgcttc gagaataatt gatttcttag
     2461 aggcaaaggt agtgaatctt tcatttgatg acatattaaa atataagcaa gagttcaaaa
     2521 aaatatgtta taaacaactt caagagttta caacaccctc tctatacaat aaaattcaaa
     2581 aatgggcaac aatgtctaaa aatgaattca tagcatttca ttacgagaca cttcaacccg
     2641 aaaagatttc ataccccttt tatttaaaga gagatctgcc taatgaaaaa gatataaatt
     2701 atggggtgga aatagaaata ccatctggca aaagaattcg cttatcaaat cattatcaga
     2761 atataatacc ataatattga ttattagcac tttataatca ttgaataaaa atagatttta
     2821 tgtaatttta aaacaaaggg ttatattatt gggcgatgga tactcatacc acgccccaaa
     2881 ataacggaat tgaactaaac agaagggata taaacatcag acaggtttac gttactatca
     2941 ggcatatcac ctcagaatca gatgaaaact ataaagaaat atctattatg gttttaatat
     3001 ttgttgataa ggatagtaac atgaacatga caaaaggtgc actcatcctc agcctttcat
     3061 ttttgcttgc cgcatgtagt tcaattccgc aaaatatcaa aggcaataac caacctgata
     3121 ttcaaaaaag ttttgttgct gttcataacc agccggggtt atatgttggt caacaagcgc
     3181 gctttggtgg gaaggttatc aacgttatca atggcaaaac ggatacgttg ttagaaatcg
     3241 ctgtattacc gttggatagc tatgcgaagc ctgatattga agccaactat cagggccgac
     3301 tgctcgccag acaaagcggc ttccttgatc cagtgaacta tcgtaatcac tttgttacca
     3361 tcctcggtac cattcagggt gaacaaccag gctttatcaa taaagtcccg tataacttcc
     3421 tggaagtgaa tatgcagggc atccaggtgt ggcatttgag agaagtggtt aataccacct
     3481 ataacctgtg ggattacggc tatggtgcat tctggccgga accgggctgg ggtgcgcctt
     3541 actacaccaa tgcggtgagt caggtaacac ctgagctggt caaataacac caccgaaaga
     3601 tgcagatgct ttctcagcat ctgcatcatg tattacataa aattaatata cagtaagcta
     3661 actattatta ttataagccc tgtcctgtta attacctttg gcaaactgat tataaagtta
     3721 atgtccgcac caggagtcgg ttatgtttct tataattacc agggatacga tgttcttcac
     3781 cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat atacagaacg aagaagagat
     3841 cgacgtaatg ttgcatcaga atgccttcgt cattattgat acattaatga ataatgtatt
     3901 tcattctaat tttctcactc aaattgaacg attaaaacct gtccatgtca ttgttttctc
     3961 cccctttaat attaaacgct gtctggggaa agtaccggtg acctttgttc cgcggactat
     4021 cactatcatt gattttgtcg cactcatcaa tggcagttac tgctctgtgc ctgaagcgaa
     4081 tgtgtcactt tcgcgcaagc aacatcaggt tctgagctgc attgcgaatc aaatgacaac
     4141 tgaagatatt ctggagaaac tgaaaatatc gctaaaaacg ttctactgcc ataaacacaa
     4201 tatcatgatg atcctcaatc ttaagcggat caatgagctg gtacgccatc agcatattga
     4261 ttatctggtg tgaatttcag gcttacggtg agtctggcta cgctgccaca cagattagct
     4321 aattgaaacg cctttcaccc ctgccatacc ttttaataat cgcaacaggt cttctatcaa
     4381 cgttgtggca tgaagctgga tatcaatggc gaccacttca tgatcttcat tttcctgcaa
     4441 cgaaactaaa tcagttttta ttttttgctg cttaaaccag tcgagcattg ataccgtatt
     4501 accgttcact aatgtcagct gtaaatggta atttttgttc atcaggcgga aggttaattg
     4561 atggaagact tccagcacca acaaggtcat cactgaacca taaataccca gttcgtacat
     4621 accgctgcca ataaccatac ctatgcggcg gtcacccaga tatccgctgc cgtcgtcaga
     4681 cctacaatat tttggttacg aacgagaatg ttacctgcac cgataaaccc gacgcccgtc
     4741 accacctgag cagcaatacg gctggggtcg agtccgacgt gatctaaaga cagcacgtca
     4801 gcaaaaccat atttcgaaac aatcataaac agggcgcttc ccatgccaat taatacatgt
     4861 gtgcgta
//
LOCUS       DAECNO010000188         4862 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.192, whole genome shotgun sequence.
ACCESSION   DAECNO010000188 DAECNO010000000
VERSION     DAECNO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.192"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..425
                     /locus_tag="KKO08_003943"
     CDS             <1..425
                     /locus_tag="KKO08_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6319992.1"
                     /translation="PRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLG
                     VYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG
                     TVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            759..3038
                     /gene="ydeP"
                     /locus_tag="KKO08_003944"
     CDS             759..3038
                     /gene="ydeP"
                     /locus_tag="KKO08_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBD6319993.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3286..3489
                     /gene="safA"
                     /locus_tag="KKO08_003945"
     CDS             3286..3489
                     /gene="safA"
                     /locus_tag="KKO08_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBD6319994.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3557..4318
                     /gene="ydeO"
                     /locus_tag="KKO08_003946"
     CDS             3557..4318
                     /gene="ydeO"
                     /locus_tag="KKO08_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBD6319995.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4720..>4862
                     /locus_tag="KKO08_003947"
     CDS             4720..>4862
                     /locus_tag="KKO08_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBD6319996.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1389 a         1038 c         1079 g         1356 t
ORIGIN      
        1 atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg
       61 gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag
      121 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
      181 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
      241 cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
      301 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
      361 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
      421 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      481 attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag
      541 tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg atgcttaact
      601 tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct
      661 tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt
      721 acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct
      781 accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      841 agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg
      901 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      961 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc tctttctttg
     1021 ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc
     1081 gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
     1141 aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg
     1201 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
     1261 gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg
     1321 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
     1381 agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga
     1441 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
     1501 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
     1561 agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca
     1621 aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca ggtcggccct
     1681 cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1741 acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa
     1801 tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1861 ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga
     1921 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg
     1981 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     2041 tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca
     2101 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac
     2161 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac
     2221 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     2281 cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     2341 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     2401 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     2461 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     2521 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     2581 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca
     2641 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac
     2701 gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2761 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2821 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca
     2881 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2941 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     3001 ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc
     3061 tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg
     3121 attaaattaa actacatact ataagatata aacttctgcc tacagctgta agaaactccg
     3181 ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac
     3241 tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     3301 gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgc aattgtggtt
     3361 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt
     3421 tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac
     3481 tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt
     3541 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa
     3601 cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa
     3661 cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
     3721 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
     3781 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
     3841 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
     3901 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt
     3961 ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga
     4021 tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca
     4081 aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg
     4141 cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt
     4201 tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg
     4261 ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt
     4321 atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc
     4381 ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca
     4441 tatttatgca cttgcataac ctgttgcatg attatttatg atctcaattc ggcattttgt
     4501 cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta
     4561 cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct
     4621 aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa
     4681 attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa
     4741 agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct
     4801 catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctcagacttt
     4861 ac
//
LOCUS       DAECNO010000189         4713 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.193, whole genome shotgun sequence.
ACCESSION   DAECNO010000189 DAECNO010000000
VERSION     DAECNO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.193"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..908)
                     /locus_tag="KKO08_003948"
     CDS             complement(<1..908)
                     /locus_tag="KKO08_003948"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBD6319997.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCI"
     gene            complement(1026..1115)
                     /locus_tag="KKO08_003949"
     CDS             complement(1026..1115)
                     /locus_tag="KKO08_003949"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6319998.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(1551..1712)
                     /locus_tag="KKO08_003950"
     CDS             complement(1551..1712)
                     /locus_tag="KKO08_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD6319999.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            2350..2673
                     /locus_tag="KKO08_003951"
     CDS             2350..2673
                     /locus_tag="KKO08_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBD6320000.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPS
                     ISPTLLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKG
                     TDDDR"
     gene            2612..3376
                     /locus_tag="KKO08_003952"
                     /pseudo
     CDS             2612..3376
                     /locus_tag="KKO08_003952"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3351..3842
                     /locus_tag="KKO08_003953"
     CDS             3351..3842
                     /locus_tag="KKO08_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBD6320001.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKVAFSEHEQSVKAELSAS
                     ARRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILIVSALLIGTSGSILWWQGQQ
                     ITDNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRL
                     KQE"
     gene            3849..4064
                     /locus_tag="KKO08_003954"
     CDS             3849..4064
                     /locus_tag="KKO08_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBD6320002.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQREN
                     AVLNERVSDLSTQVLSLSEQLRRLSGN"
BASE COUNT         1268 a         1081 c         1255 g         1109 t
ORIGIN      
        1 tagatgcatt tagcatatga gttgaaaaaa tagaggttaa atttcttatt aattattaat
       61 gctgctttgt aaatgataat gcttatgcac attaaaggta ttattgataa ctggtgattg
      121 actatgtttg ttttgaactc attaggccct attaataata ctgtcaatat acatataaca
      181 accgatagaa taactgtggt gtttttttgt aatttatttt tgtatttaaa tgcaagcatc
      241 ccaaccataa actcaggaag tcggcaagca aaaaaccaaa atgggaagtt taaaaatgat
      301 gatgagatat ttgatattgg gaatgttgta attgttatca caaataaaaa aaatatgtta
      361 cgtttaaatg taaaggtatt cataacgtga aaaactatcg gtgatatcac gtacagcata
      421 ataacgcacc caataaacca ctcgcctgta aggaaaagaa aatcataaga gaaatgggcg
      481 tttaatatac catcaagggc aaaaaatact ggaattattc tagtgtactg gcctcctgtt
      541 gtcattattg atacgagagg gactgtatta attatctctt tatttaaata agacaataaa
      601 attaagagta aagagataaa aaagtaagtg ataatataag gagtaaggat ccttactcct
      661 ttgtcaagga aataatcaat ggtattcttt ctatctagtg aaataaatgc caaatatcca
      721 gatataaaaa aaaacaagct cacacctacc tggccaagaa cccctccata aaatgatggt
      781 atgaaagata gcctggtttg gctaaatatg tagccataat gagatataat aacaaaaatg
      841 caggctgtta ctcgcaatgt atcaattgac ggatctctat tagacataac ctttatttcg
      901 ctgttcaaat tatcgccttt agttgacgat gtaaaattaa cgctataaat aagctacaca
      961 ttctagagca gcgaaaggat gattcaagta tttttaataa taaaacgcat tgataatgtt
     1021 tatgttcaga tggtgctgat agctttgtca tgctgttcca ctgatgttga taacgacaga
     1081 gacttttgtc cttttcttgt tagatgctgc ctcagattcg gattatggcg ttaaaggggt
     1141 ttgacgctca accgaacggt ttcacacgtt aagagctttt tgtctgataa atcaaaatat
     1201 aatggacatt gaaaaacgcc ctcaaaacgg tgtcttttta atcgaagatt ggcacaaaac
     1261 ggacaccacc gtccattagt cgatttttta gacggctttt ctcgcctaac gtgagttttc
     1321 gttcggttga gcatcagacc ccttttatat tgtagatttc tatttttaga cagttggaac
     1381 aggacaatta acaacagatc tacttgagat catttgcttg tcgtcgtagt ctctttccca
     1441 gaaacgaaaa aaccgccctg caaggcggtt tctcgaaggt tctcagagcg accaactctt
     1501 tgaaccaagg taactggctt atgaggagcg cagtaaccaa atctgtcctt tcagtttagc
     1561 cttaacaggc gcataacttc aagactaact cctctaaatc cgttaccaat ggctaccgcc
     1621 actggcgatt tgtcgtgtct ttccgggttg gactcaagtt gatagttacc ggaacgggcg
     1681 tggtagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
     1741 ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc
     1801 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg gaaacgcctg
     1861 gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga ttctgtgatg
     1921 cttgtcaggg gggcggagcc tatggaaaaa cggcagcgcc gcatcctttt tctgccccgc
     1981 ttgtcatgcc gtatgccttt gctcttatcc gcgagcaggt aacgcccgcc gcagtcgaac
     2041 ggcaggagcg gcagcgaccg agtgagcgag gaggcgtcat atcatcgtaa cctccatttt
     2101 ctcctgtacg ccctgcagct gcattttccc ctgtcacatg aagcactttc attaagtgtt
     2161 actccgccgt aacatattaa gccagtatac actccgctag cgctgcatga ctggttcagg
     2221 gctgcgcccc gaaacccgct aaaagccact gacgcgcctg cggcttgtcc aaccccggcc
     2281 gaccgggaaa ggttttcccg cccgtcccgg cgttatcagg agaccggttt gtcagagaaa
     2341 cggaacaaga tgctcaccat gtgggtgacg gaggacgagc accggcggct gcttgagcgc
     2401 tgcgacggca ggcagctggc agcgtggatg cggcagacct gcctggacga aaagcctgcg
     2461 cgtaccggaa agcttccctc gatctcgccg acgctgctgc gccagcttgc cggcatgggc
     2521 aacaacctga accagatagc ccgccgcgtt aacgccggcg gcggcaccgg ccatgacagg
     2581 gtgcagattg ttgcggcgct gatggccatc gatgccggcc ttgagcggct gcggcacgcc
     2641 gttcttgaga aaggaacgga cgatgatcgt taaatttcat ccacgtggtc gcggcggtgg
     2701 cgccgggccg gtggactatc tgctgggtaa agaccggcag cgtgaaggcg ccagcgtcct
     2761 gcaggggaag ccggaagaag tccgggagct gattgacgcg tcgccttacg tgaagaaata
     2821 cacctccggc gtactgtcgt ttgcggaggc cgatctgcct cccggccagc gggaaaaact
     2881 gatggccagc ttcgagcggg tactgatgcc cggactcgat aaagatcagt acagcattct
     2941 gtgggtggag cacgcggaca aggggcgtct ggagctgaac tttctgatcc ccaataccga
     3001 gctgctgacc ggcaggcggc tccagccgta ctacgaccgc gccgaccggc cgcgcatcga
     3061 tgcctggcag accatcgtga acggcagact ggggctgcac gacccgaacg caccggaaaa
     3121 ccgccgggcg ctggtcacgc cgtccggact gccgaaaacg aagcaggagg ccgcagaatc
     3181 catcacgcgc ggactgctga accttgcttc gtccggggag ctgaaaaccc gtcaggacat
     3241 cactgaggcg ctggaacggt caggttttga ggtggtgcgc accacgaaaa gcagtatcag
     3301 cattgccgac ccggacgggg ggcgaaacat ccgactgaag ggagccatct atgagcagtc
     3361 ttttaacgct ggcgaaggac ttagagcagc aatcgaaagc gcagaagcag agtaccggcg
     3421 agatgctgaa agtcgcattc agcgagcacg agcagtctgt caaagcggaa ctgagcgcaa
     3481 gcgcgaggag aatcagcgac gccatcctcg cccacgagca gagtatgagc gaggcgatgg
     3541 agaaaaaccg gcggagcgta ctgcggactg ccgggcggac atggctcacc atcctgattg
     3601 tgtcagcgct gctgatcggc acgagcggga gcatactgtg gtggcagggg cagcagataa
     3661 ccgacaatta cacgcatctt cgtcagcagg aagataccct ggcgaaaatg accgcccgga
     3721 cgtggggagt gcgctatcag gagagcagcg acggccggag gtttcttatc ctgccgccgg
     3781 ggatgcaaac cgaagccatc ccttacgacg ggacgacatg gatccgcctg aaacaggagt
     3841 agcggaagat gacagagctg gaaaagcagt tgctgagcgc attagagcag ctacagcagg
     3901 actactcgca aaggctggac gaatgggaga gcgccttcgc ggaatggcgg agcatgtgtg
     3961 ggcttatgca acgggagaac gcggtgctga acgagcgcgt gtcggacttg agcacgcagg
     4021 tgctgagttt aagcgagcag ctgcgccgct tgtccgggaa ctgaatgcca ccgagttccg
     4081 gcagagggag aatgagtatc agaaaacact ggagctggag cgccagccgc agaaaaccta
     4141 ccgcgggccg acactttaaa ccatccaggc agctgacaac ccgcacgaag aggggctctt
     4201 acttctcccg ctaaataggt tcgtgatcaa aaagatcacg gcctatacct gggggggaat
     4261 tatgaaattg cttaacgtca ttgaagctgt acttaactgc ctgtatctgg cggccgggct
     4321 gtgggactgg ctggagaagc atttacctgt ttttaaggcg ttagcgctga ttttagtgtg
     4381 actttacacg ccatgcatct ggctgcatgg ttatgcagtc gggtaaaatt tatctgtcgc
     4441 gatcggggtg gtttaccggg tgggttgttg ttggttttgg ctgactgccg ccccgttcgc
     4501 ggcgaagctg tccggggcgg tgcgggcaat gagatgttat ggtaaattca agtcttagat
     4561 tttatgatgt ttaattagct ttttttgcgt ttataatgtt catctttttt attaaaattg
     4621 attcaatctt tgatgtgggt attaatattt tctttgacaa atagaaagtt aaaaaaaaca
     4681 ccattaatag tataaataaa tacccaaaaa cag
//
LOCUS       DAECNO010000190         4636 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.194, whole genome shotgun sequence.
ACCESSION   DAECNO010000190 DAECNO010000000
VERSION     DAECNO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.194"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..311
                     /locus_tag="KKO08_003955"
     CDS             <1..311
                     /locus_tag="KKO08_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBD6320003.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="KKO08_003956"
     CDS             468..1217
                     /locus_tag="KKO08_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBD6320004.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="KKO08_003957"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="KKO08_003957"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBD6320005.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KKO08_003958"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="KKO08_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBD6320006.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="KKO08_003959"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="KKO08_003959"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBD6320007.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="KKO08_003960"
     CDS             4084..4557
                     /locus_tag="KKO08_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBD6320008.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1162 c         1138 g         1098 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DAECNO010000191         4610 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.195, whole genome shotgun sequence.
ACCESSION   DAECNO010000191 DAECNO010000000
VERSION     DAECNO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.195"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            224..2647
                     /gene="pgaA"
                     /locus_tag="KKO08_003961"
                     /pseudo
     CDS             224..2647
                     /gene="pgaA"
                     /locus_tag="KKO08_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2656..4149
                     /gene="pgaB"
                     /locus_tag="KKO08_003962"
     CDS             2656..4149
                     /gene="pgaB"
                     /locus_tag="KKO08_003962"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HBD6320009.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREKFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNHLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIR"
     gene            4153..4610
                     /gene="pgaB"
                     /locus_tag="KKO08_003963"
                     /pseudo
     CDS             4153..4610
                     /gene="pgaB"
                     /locus_tag="KKO08_003963"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="in Yersinia this protein is involved in biofilm
                     formation and hemin adsorption; related protein PgaB in
                     Escherichia coli is an outer membrane N-deacetylase
                     involved in poly-beta-1,6-N-acetyl-D-glucosamine (PGA)
                     export; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase"
BASE COUNT         1318 a         1032 c         1061 g         1199 t
ORIGIN      
        1 tttatgccgt attcctgaag atcctcatca ttggaatgga ttttcgggcg agaaaaggat
       61 tttatatgga cactctgctc atcatttctt cttctcatca tcaacaattc acgtctctct
      121 tccgcgttta ataacggatt atgaggtgca aaaatatctt tcttttcagt tacctgtaat
      181 tagatacaga gagagatttt ggcaatacat ggagtaatac aggatgtatt caagtagcag
      241 aaaaaggtgc ccgaaaacca aatgggcttt gaaacttctt actgccgcat ttttagcagc
      301 gagtcccgcg gcgaagagtg ctgttaataa cgcctatgat gcattgatta ttgaagctcg
      361 caagggtaat actcagccag ctttgtcatg gtttgcacta aaatcagcac tcagcaataa
      421 ccaaattgct gactggttac agattgcctt atgggccggg caagataaac aggttattac
      481 cgtttacaac cgctaccgtc atcagcaatt accagcgcgt ggttatgcag ctgtcgccgt
      541 cgcttatcgt aacctgcaac aatggcaaaa ctcgcttaca ctgtggcaaa aggcgctctc
      601 tctggagccg caaaataagg attatcaacg gggacaaatt ttaaccctgg cagatgcggg
      661 tcactatgat actgcgctgg ttaaacttaa gcagcttaac tctggagcac cggacaaagc
      721 caatttactc acagaagcct atatctataa actggcgggg cgtcatcagg atgaattacg
      781 ggcgatgaca gagtcattac ctgaaaatgc atctacgcaa caatatccca cagaatacgt
      841 gcaggcatta cgtaataatc aacttgctgc cgcgattgac gatgccaatt taacgccaga
      901 tattcgcgct gatattcatg ccgaactggt cagactgtcg tttatgccta cgcgcagtga
      961 aagtgaacgt tatgccattg ccaatcgcgc cctcgcccaa tacgctgcat tagaaattct
     1021 gtggcacgat aacccagacc gcactgccca gtaccagcgt attcaggttg atcatcttgg
     1081 cgcgttatta actcgcgatc gttataaaga cgtcatttct cactatcagc gattaaaaaa
     1141 gacggggcaa attattccgc cctaggggca atattgggtt gcatcggctt atctcaaaga
     1201 tcatcagccg aaaaaagcac agtcaataat gaccgagctc ttttatcaca aggagaccat
     1261 tgccccggat ttatccgatg aagaacttgc ggatctcttt tacagccacc tagagagtga
     1321 aaattatccg ggcgcgctaa ctgtcaccca acataccatt aatacttcgc cgcctttcct
     1381 tcggttaatg ggcacgccta cgagcatccc gaatgatacc tagttacagg ggcattcgtt
     1441 tctctcaacc gtagcaaaat atagtaatga tcttcctcag gctgaaatga cagccagaga
     1501 gcttgcttat aacgcaccag gaaatcaggg actgcgcatt gattacgcga gtgtgttaca
     1561 agcccgcggt tggcctcgtg cagcagaaaa tgaattaaaa aaagcagaag tgatcgagcc
     1621 acgtaatatt aatctggagg ttgaacaagc ctagacagca ttaacgttac aagaatggca
     1681 gcaggcagct gtcttaacgc acgatgttgt cgaacgtgaa ccgcaagatc ccggcgttgt
     1741 acgtttaaaa cgtgcggttg atgtacataa tcttgcagag cttcgtatag ctggttcaac
     1801 aggaattgat gccgaaggcc cggatagtgg taaacatgat gtcgacttaa ccaccatcgt
     1861 ttattcacca ctgctgaagg ataactggcg cggttttgct ggattcggtt atgccgatgg
     1921 acaatttagc gaaggaaaag ggattgttcg cgactggctg gcgggtgttg agtggcggtc
     1981 acgtaatatc tggctcgagg cggagtacgc tgaacacttt ttcaatcatg agcataaacc
     2041 cggcgcgcgc ctgtctggct ggtatgattt taatgataac tggcgtattg gttcgcaact
     2101 ggaacgcctc tctcaccgcg ttccattacg ggcaatgaaa aatggtgtta caggcaacag
     2161 tgctcaggct tatgttcgct ggtatcaaaa tgagcggcgt aagtacggtg tctcctgggc
     2221 tttcactgat ttttccgaca gtaaccagcg tcatgaagtc tcaattgagg gtcaggaacg
     2281 catctggtct tcaccatatt tgattgtcga tttcctaccc agtctgtatt acgaacaaaa
     2341 tacagaacac gataccccat actacaaccc tataaaaacg ttcgatattg ttccggcatt
     2401 tgaggcaagt catttgttat ggcgaagcta tgaaaatagc tgggagcaaa tcttcagcgc
     2461 aggtgttggt gcctcctggc aaaaacatta tggcacggat gtcgtcaccc aactcggcta
     2521 cgggcaacgc attagctgga atgacgtgat tgatgctggc gcaacgctac gctgggaaaa
     2581 acgaccttat gacggtgaca gagaacacaa cttatacgtt gaattcgata tgacattcag
     2641 attttaagga taaatatgtt acgtaatgga aataaatatc tcctgatgct ggtgagtata
     2701 attatgctca ccgcgtgcat cagccagtca agaacatcat ttataccgcc acaggatcgc
     2761 gaatctttac tcgccgagca accgtggccg cataatggtt ttgtggcgat ttcatggcat
     2821 aacgttgaag acgaagctgc cgaccagcgt tttatgtcag tgcggacatc agcactgcgt
     2881 gaaaaatttg cctggctgcg cgagaacggt tatcaaccgg tcagtattgc tcaaattcgt
     2941 gaagcacatc gaggaggaaa accgctaccg gaaaaagcag tagtgctgac ttttgatgac
     3001 ggctaccaga gtttttatac ccgcgtcttc ccaattcttc aggccttcca gtggcctgct
     3061 gtatgggccc ccgtcggcag ttgggtcgat acgccagtgg atgaacaagt aaaatttggc
     3121 gatgagatgg tcgatcgaga atattttgcc acgtggcaac aagtgcgaga agttgcgcgt
     3181 tcccggctcg ttgaggtcgc ttctcataca tggaattctc actacggtat tcaggctaat
     3241 tccaccggca gcttaatgcc tgtatatgta aatcgtgcat attttactga ccacgcacgg
     3301 tatgaaaccg cagcagaata ccgggaaaga attcgtctgg atgctgtaaa aatgacggaa
     3361 tacctgcgta caaaggctga ggtaaatcca cacgtttttg tttggcctta tggcgaagcg
     3421 aatggcatag cgatagagga attaaaaaaa ctcggttatg acatgttttt cacccttgaa
     3481 tcaggtttgg caaatgcgtc acaattggat tccattccgc gagtattaat cgccaataat
     3541 ccctcattaa aagagtttgc ccagaaaatt attaccgtac aggaaaaatc accacaacgg
     3601 ataatgcata tcgatcttga ttacgtttat gacgaaaacc gccagcaaat ggatcgcaat
     3661 attgatgtgc taattcagcg ggtgaaagat atgcaaatat caaccgtgta tttgcaggca
     3721 tttgctgatc ccgatggtga tgggctggtc aaagaggtct ggtttccaaa tcatttgcta
     3781 ccaatgaaag cagatatttt tagtcgggtt gcctggcaat tacgtacccg ctcaggtgta
     3841 aacatctatg cgtggatgcc ggtattaagc tgggatttag atcccacatt aacgcgagta
     3901 aaatacttac caacagggga gaaaaaagca caaattcatc ctgaacaata tcaccgtctc
     3961 tctcctttcg atgacagagt cagagcacaa gttggcatgt tatatgaaga tcttgccgga
     4021 catgctgctt ttgatggcat attgttccac gatgatgctt tgctttcaga ttatgaagat
     4081 gccagtgcac cggctatcac ggcttatcag caagcaggct ttagcgggag tctgagcgaa
     4141 attcgataaa acccggagca atttaaacag tgggcccgct ttaaaagtcg tgcgttaact
     4201 gacttcactt tagaacttag tgcgcgcgta aaagccattc gcggtccaca tattaaaact
     4261 gcacgaaata tttttgcact tccggtaata caacctgaaa gtgaagcctg gtttgcacag
     4321 aattatgctg atttcctaaa aagctatgac tggaccgcta ttatggctat gccttatatg
     4381 gaaggtgtcg cagaaaaatc ggctgaccaa tggttaatac aattgaccaa tcaaattaaa
     4441 aacatccctc aggctaaaga caaatctatt ttagaattac aggcacaaaa ctggcagaaa
     4501 aatggtcagc atcaggctat tccttcgcaa caactcgctc actggatgag cctattacaa
     4561 ctgaatggag tgaaaaacta tggttattat cccgacaatt ttctgcataa
//
LOCUS       DAECNO010000192         4577 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.196, whole genome shotgun sequence.
ACCESSION   DAECNO010000192 DAECNO010000000
VERSION     DAECNO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.196"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..356
                     /locus_tag="KKO08_003964"
     CDS             <1..356
                     /locus_tag="KKO08_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6320010.1"
                     /translation="FVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRFH
                     DLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNG
                     TNTAQEENVVYLNAR"
     gene            618..980
                     /locus_tag="KKO08_003965"
     CDS             618..980
                     /locus_tag="KKO08_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBD6320011.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            977..1897
                     /locus_tag="KKO08_003966"
     CDS             977..1897
                     /locus_tag="KKO08_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBD6320012.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1900..2851
                     /locus_tag="KKO08_003967"
                     /pseudo
     CDS             1900..2851
                     /locus_tag="KKO08_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI"
     gene            2878..3118
                     /locus_tag="KKO08_003968"
                     /pseudo
     CDS             2878..3118
                     /locus_tag="KKO08_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3115..3420
                     /locus_tag="KKO08_003969"
     CDS             3115..3420
                     /locus_tag="KKO08_003969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320013.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3654..4094)
                     /locus_tag="KKO08_003970"
     CDS             complement(3654..4094)
                     /locus_tag="KKO08_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HBD6320014.1"
                     /translation="MRITLQWDINEMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINSQSVTKMITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAPLQ
                     DAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
     gene            complement(4066..>4577)
                     /locus_tag="KKO08_003971"
     CDS             complement(4066..>4577)
                     /locus_tag="KKO08_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320015.1"
                     /translation="CSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVK
                     TALENLGLGEAAKRNVGTGANQIPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPN
                     VSSTYGMRFPMTFSRVLAVTVTLNVTGASGQPTVSATNVQNTGFDITVSPGSGYGSSA
                     DAYYIAMGY"
BASE COUNT         1309 a          937 c          900 g         1431 t
ORIGIN      
        1 tgtttgtcca caccaaacct gcctaccgaa gcgacggaat aaaaacagca gcggtaagga
       61 agatgagaac cgacagcaac aaggcatgga agggagcgtt aaagcgggca ggcattagca
      121 acttccgctt ccatgacctg aggcatacct gggcaagctg gctggttcag tccggtgtct
      181 ctcttcttgc gcttaaagag atgggaggat gggaaactct cgaaatggtt caaagatacg
      241 cccacctttc agccgggcat ctcaccgagc acgcgagcaa aatcgatgcg attataagtc
      301 gcaatggcac aaatacggca caagaggaga acgtagttta cttaaatgcg aggtaactta
      361 ttgatttaaa tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaattata
      421 agaatccgct tgtaattcaa aggattatcc catcaacact gcgctcacac gtcccaccac
      481 atcaaaacat gtaaagcctt gcaagccatt gtgaggcctt atgtgtctca gttttgtctc
      541 atcagacata gcaagtatcg atcaattgag acttggatga tagacttcat gcctttcaga
      601 gctcattgat taaataaatg ttaaagttat ttgtaaagta cacatctata ggtgtgctga
      661 acacacttat acactgggtg gtttttggtg tatgtatcta taccgcacat accagtcagg
      721 ctctggcaaa cttcacaggt ttcgtagtgg ctgtgagctt tagcttcttc gcgaatgcaa
      781 gattcacatt caaagcatcg actacagcga tgcgctacat gttatacgtg ggatttatgg
      841 gaatattgag tgtgattgtt ggttgggcag ctgataaatg ctcacttcct ccaatagtca
      901 ctcttatcac cttctccgcc atcagtctgg tgtgcggttt cgtctattca aagttcattg
      961 tctttaggga tgcgaaatga aaatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1021 gatacctgtt ttctataaaa cggtacgtga attccaagag ttgaagccat atgaagtaga
     1081 aattgtattc ataaatgacg gaagtaaaga tgccacagag tcaattatta acgcgctggc
     1141 tgtttcagac ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1201 cttatttgca gggttagacc atgcaagcgg cgatgctgta attcctattg atgtcgacct
     1261 gcaagaccca attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgagat
     1321 ggtgcttgct aaacgttcag accgctcaac tgatggacga ctgaaacgta agacagctga
     1381 gtggttctat aaattacaca acaaaataag caccccaaag atcgaggaaa atgtcggaga
     1441 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctgttgcctg agcgcaatct
     1501 tttcatgaaa ggcatactga gctgggtggg tggtcagacg gatgtcgttg aatatgtacg
     1561 cgcagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1621 ggaaggtatc acaagttttt caacctttcc tcttcgcgta tggacttata taggcttgtt
     1681 tgttgcaagc atttcatttt tatatggtgc atggatgatt atagacaccc ttgtctttgg
     1741 taacccagta cgcgggtatc cctccctgct tgtatcaata cttttcttgg gtggagtgca
     1801 actgatcggg attggtgttc tcggtgaata tataggtaga atttacttag aaacaaaatc
     1861 aagacctaag tatattttga agaaaggtag aaaataacca tgagcatatg tattaaacaa
     1921 tcagcattaa aaatattatt ggctttatca gcactcctaa taacttggtt gactaccaga
     1981 tactttccag ttgaaccgga tgttgctaac agcccaattg tatggaggca tattttagag
     2041 aatggtatat catcaatcca cgactggaaa ccaaccgtgg ataattggta tttcaccgtt
     2101 tacccaatta actttttatt ttatatgctg ttaggtgatg atggcttagt agcattaaga
     2161 ttatcaactg cagttttctc tattgctata gtaatagcag cgatgttaac gctacgtaag
     2221 gcattcgggt ttacccctgc tttattttct attatacttc tatctctaat accatatttt
     2281 tcatacactt atggttttgt atcacatcct ttttctcata attcaacaaa cgctttcggc
     2341 tttttatgct tgcttatatc tgttttcaac atacagtata aaaatatatt tatcactctc
     2401 ctcttaagtt tgacagcatt attttccagc gtatccgatc cgtggtttac agctgcattt
     2461 tttattccac tattaatatc ttacttttta ttctctgtat gggataaaaa attatttaag
     2521 catactgcat taatactatt tgcatgttta atctctcttt ccaatgtatt gcaaaatcta
     2581 cttaacatac ctccccacca atttgaaatt gtatcactca atgatatgat tttaaatgca
     2641 aaatggtgca ttctgttaat aggaaaaagt cttaatttat tagttgtcga caataacgca
     2701 acatcttacg cctcatttgt catttggttc attgccatta ttacatctgc gtggtttgtt
     2761 ttatcggaca ataaaaaaaa cacataccgt atttatattg tgttattttc attattatct
     2821 atcgcaggta ttgtctcatc attcatactg agctataaat cccctgatta tattagcatg
     2881 cgtttcttta tgaatgttac ctgtttcgca ctgatattgt gctgtatcgg tacatcaaca
     2941 aaagcaaaga tattatttta tctaatcgca tttttatttt cgatcagttc aattaaatct
     3001 tacacaaata atgcttcacc attacacgat caggagaaga ttgttaaatc atacattgat
     3061 tttttaaaaa aaaataatct ccattatggg tacggctctt tttgggattt gtcaatgact
     3121 gtaaattggt tatctggtgg ggacatacag ataactcctg ttttctttaa tgctgattcg
     3181 gggaaaatta actttactgg cgtgagacaa cagacactgg cttcttggca tagcaaggag
     3241 gcgttcaatt ctgctcctga gcgacagttt atagcagttt ccatagctaa cgaacctgaa
     3301 cgatgtaaag aaatgacatc ctgccttgca ggtattcaag aacaattagg taagcctgac
     3361 gaagttttga actttgaagg aagagtaata ttagtattta acaaaaaact taacctatag
     3421 taaattgccc cctaccatat aaaccacact aacgaatgaa taacccgtct ttttcgacgg
     3481 gttatatccc tatcaggaat aaaaatggag tggttgccgt atacaacaaa ccctcattta
     3541 gagctatatt ctagagtata tgagatacta taaagttgaa ggagctatca ttagaaaaac
     3601 catatgctgt tataattaga tgtctcgctt ttcatccaat aacgaatttt ctcttataga
     3661 ttgctctctg gttttttcgg ccactctgta ttttttatat caacacgcat caatagtgcg
     3721 cgatattttt tccatgccag taacagtttc gtttcatcat ctgttgccat atctaaatca
     3781 accgcatcct gaagcggcgc tattacattc gttgcctctg ctatcagtgc agatttcttt
     3841 tcattattta ttaaggctaa ttgttctggt gttggagaag gacggtcaac aatgacaggg
     3901 tgaccattct caccacaggt aatcattttt gttacactct gcgaattaat cagtgttttc
     3961 cactctgatt ccgagatttc cactgcatca tcaggaatat tggtgccgtg aaattcagtc
     4021 gaataaaatc cgtttgtcga tgcagaataa aaatatgaca tttcgttaat atcccattgc
     4081 aatgtaatac gcatcagcag atgaaccata tcctgaaccg ggcgacactg taatatcaaa
     4141 tccagtgttc tggacatttg tcgccgatac agtcggctgc cctgacgcgc cagtaacgtt
     4201 caatgtaact gtgacagcaa ggactcttga gaatgtcatc gggaacctca tcccgtatgt
     4261 tgaagataca ttaggggagc cattcaactg accgcactgt aaaattaatc cagacggaaa
     4321 tttttgcgca gcagccgttg cggtattact tgacgcgaac aggctcatat caggtatctg
     4381 attcgcccct gtacctacat tccttttagc tgcttctccc aaaccaaggt tttcgagagc
     4441 cgttttcacc gtgccatccg atttgatatc gccaaacgga ttcttgcggc ttaacagcag
     4501 cgcgcgaagt gcggtaagca actgatcgtg ccgccccttc tccaggctgg caccggatgc
     4561 ctccaccacg ctgcaaa
//
LOCUS       DAECNO010000193         4557 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.197, whole genome shotgun sequence.
ACCESSION   DAECNO010000193 DAECNO010000000
VERSION     DAECNO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.197"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            291..851
                     /locus_tag="KKO08_003972"
     CDS             291..851
                     /locus_tag="KKO08_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HBD6320016.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALMNKVS
                     ENTKQRNLFSKKMTLCISLFLFLGGR"
     gene            794..1183
                     /locus_tag="KKO08_003973"
                     /pseudo
     CDS             794..1183
                     /locus_tag="KKO08_003973"
                     /inference="COORDINATES: protein motif:HMM:NF028467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
     gene            1163..1720
                     /locus_tag="KKO08_003974"
                     /pseudo
     CDS             1163..1720
                     /locus_tag="KKO08_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            1818..3427
                     /locus_tag="KKO08_003975"
                     /pseudo
     CDS             1818..3427
                     /locus_tag="KKO08_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3528..4019)
                     /locus_tag="KKO08_003976"
     CDS             complement(3528..4019)
                     /locus_tag="KKO08_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FidL"
                     /protein_id="HBD6320017.1"
                     /translation="MTQRYFLFSGIILCAFIAAILSHIAFHHSNEPAEQNISCNAHVI
                     NFTDHTKMDQYFSLNMISENNTGNMYLTGAYTVDGKKMGFIRRYVSFTYKKFRDTVYL
                     TTVKIQKIQKDDNISDEILENITSDFFIKADTSINYYVTRQNDYNYIFSTGRTPRFIC
                     ITH"
     gene            complement(4024..4200)
                     /locus_tag="KKO08_003977"
     CDS             complement(4024..4200)
                     /locus_tag="KKO08_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001411776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6320018.1"
                     /translation="MLSPIIEKLQKMEVNCKIHPYIYIATNDSMSGFYAFTCSQMLNS
                     ESPNNCNSLYYKAE"
BASE COUNT         1399 a          728 c          901 g         1529 t
ORIGIN      
        1 cccaatgcct ttttcaaaat aaattaggat taaaataatt aaatcagtaa attccgatgc
       61 atgatttcgg atttttcgaa agtcctgatg aaaggctgcg ctacggcact cacggattta
      121 attgttattc ctaatgtatc tatgaattaa tgttttataa ggatttttta taaccattta
      181 taagtatttt caagcctggc ttgttgcaaa caatgtataa agcacttagg caataataat
      241 tacattcagc aactatcatc atcggtattg tttgtgggcg gaatttcata ttgacaacag
      301 tacagttctt atatctatta ataatagaaa gagatctaca acctacagat tggtgtagct
      361 ttatggaaaa agactatttg agaattagta gtactgtatt agtgagctta ttgtttgggc
      421 ttgctttggt tttggtgaat agttggttta atcagccagg cgttgaggaa gttgttccac
      481 gatcaaccta tctgatggta atgattgctt tattttttat cgatactgtt gcatttattt
      541 ttatgcagtt gtatttcatt tatgaccgta ggcaattttc aaactgtata cttagtttgg
      601 cttttctgag ttgtttgatt tactttgtta aaaccgtcat tatcattcag caaattattg
      661 aggggcgttt gacaagcagt gttgtccaaa atgacattgc aatctattat ttgtttcgtc
      721 agatgagttt gtgcatatta atatttctgg cattgatgaa taaagttagt gaaaacacaa
      781 aacagcgcaa tttattttcc aaaaaaatga ctttgtgcat aagtttgttt ttgtttttgg
      841 ggggccgata gttgctcaca tactttctag tcattatgag agctacaatt tacacattgc
      901 cgagttaacc aatgaaaatg gtcaggtggt ttggaaagcc tcatatgtta ccataatgat
      961 tttcatgtgg ttaaccttgc tttcggttaa tctatacttt aatggtttac gctatgatat
     1021 ctggaatgga gtgacggtga ttgctttttg tgccgtactg tacaatatct ctctgttatt
     1081 tatgagcagg tatagcgtgt ccacttggta tattagtcgc actattgaag tagtcagtaa
     1141 gttaaccgtt atggtaatat ttatgtgcca tatattcagt gcgctacgag taacaaagaa
     1201 cattgcacat cgcgatccct taaccaatat atttaacaga aattattttt ttaatgaact
     1261 gacagttcaa tcagcatcag ccaaaaaaac gccttattgt gtcatgatta tggatatcga
     1321 ccacttcaaa gaagtcaacg acacctgagg gcatccggtt ggcgatcagg tgataaaaac
     1381 agtggtgaat atcattggga aaagcatacg accagatgac cttttagcgc gcgtcggcgg
     1441 cgaagagttt ggcgtcttgc tgacggacat cgatactgaa cgtgcgaaag ctttagcgga
     1501 aaggattcgg gaaaatgttg agcgtttaac tggcgataat cctgaatacg ctataccaca
     1561 aaaagtgacg attagtattg gcgctgttgt gactcaggaa aatgcgttaa acccgaatga
     1621 gatctatcga ctggctgata acgcactata cgaggcgaaa gagaccgggc gtaataaggt
     1681 ggttgtgagg gatgtggtga gtttttgtga gtcaccataa agcggcattt tagcgggtga
     1741 ttttggtaag cgctaaatag ctattcgcag acttgcgtaa caattttcag aaatatcata
     1801 gtactatatg atcttaaatg cttatcattg atcaatctgc tatcacccgc ttttactggc
     1861 aattgtacta taatttctta ggttgcatca ccctgatgcg tggggaaaca tgtatggtat
     1921 tcaacaaaaa aatgtttgta ctcattataa taccaggcat tctgggggtg ttactatcgt
     1981 ttgctatgtc aatatttcaa atgaatagag atacgacaat taccgccggt attctgttaa
     2041 aacagcttga taatgttacg caaattgtcc agcatacgac aaagcttacc tctattctgg
     2101 tgatgaagcc ctgcaaagac attcttgaac agttgattgc taacggagca ttgactccct
     2161 atgttcgaac aacgggtctt atagaaaata atttccaaat atgttcttca gttagcggtt
     2221 tcaaaaaaat gaatgttaac gatgtttacg gtacgagctt tcacaataaa aataaagaat
     2281 cacgtatagt atcaattagc ggtacaagtt ttgtgccagg taaaacagca atagtgttcc
     2341 ttatgccaat agggaatgat atgacagctt ttagtattgt agagtcacga tatatctatg
     2401 atctaatgga tgttcttgat gatgagaatg atgattcatt ttctcttcgt tttactgaag
     2461 ggcctgcaat tattagcggt gtaaataata atgacaggtt atatatgtta aaaagagatt
     2521 ttaattctgc gatcagtcag gctcgtctta cagttactac gccgatgata tcgttatatc
     2581 cctatatcat tagcaatgtg ctttatattt tgccgttatc tattattctc tcttttattc
     2641 tctatttctt atggcagcac tggatgtcac ggaaaatgtc gctggcagaa gaaattaaaa
     2701 aaggaatgtc atccggagaa ttttctgtgc attatcaacc agtgtgcgac acgacaacca
     2761 aagcgtgtct gggggttgaa gcgttaatgc gctggcaacg cgaagatggt aaaaatatat
     2821 ctccggtagt ttttatccgc gctgcagaag aggaaaattt aataataccg ttaactaaac
     2881 atctctttga actgattatt caggatgtac aaagctggaa agtgaaaaag ccgtttcacc
     2941 ttgggataaa tattgccgcg gaacatcttg cacatcctga ctttgttggt gatgttttac
     3001 atattaaaaa tgcaatatct gataaattta atattgtcct tgagattacc gaacgcaatc
     3061 tggttgaaga cactgatctt gcattacaga aaataaatga attaaggatc catggctgtg
     3121 aatttgcagt cgatgatttt ggcacgggat attgttccct tggccttctg caaaagttgt
     3181 ctgttgatta tcttaaaata gataaaagct ttatagacac acttactaca gcggaagacg
     3241 aaacgccagt tttagatatt attattaagc tgagtaatcg gctcaatttg ataacgattg
     3301 cagaaggtgt cagtactcct caccagcaga atatcttatt cataataacg tgacattagt
     3361 tcagggatac ttatatgaaa aaccaatgaa agcgacagag ttttatcagt ggtatgtaaa
     3421 cagataaata attaaaaata ttggaatggt tttgtattgt cttaaaatat aaacaaagca
     3481 atcagaatgt tattaactga tgacaaacgc caagatcaac aatgctgtta gtgagtaata
     3541 catatgaatc gtggtgtcct gcctgtagag aatatgtagt tataatcatt ttgacgcgta
     3601 acataataat taattgaagt gtctgctttt atgaagaaat cagaagtgat attttcaagg
     3661 atttcatcgc tgatgttatc atctttttgt attttttgga ttttaactgt ggtgagataa
     3721 acggtatcac ggaatttctt gtatgtaaaa ctcacatatc ggcggatgaa gcccattttt
     3781 tttccatcta ccgtgtatgc gcccgtgaga tacatgtttc ctgtgttatt ttcagaaatc
     3841 atatttaacg aaaaatattg atccattttt gtatgatcgg tgaaattaat aacatgggca
     3901 ttacatgata tgttttgctc tgctggttca ttggaatgat gaaacgcaat atggctcaga
     3961 attgctgcaa taaaagcaca taaaattatt cccgaaaaca gaaaatatct ttgagtcata
     4021 tgattactcc gctttataat agagagagtt gcaattattt ggtgattcag agtttaacat
     4081 ttgactacag gtgaaagcat aaaacccgct catgctatca tttgttgcaa tgtaaatgta
     4141 aggatgtatt ttgcagttga cttccatttt ctgcaatttt tcaataattg gtgatagcat
     4201 gtctacggaa ttaaagtaat tattatcata gaaataactg catccttcct gagtagggtg
     4261 ttgagcataa tttgagaaag catctatttt ttttatagtg tgagatttgc tttgaaaagc
     4321 catatcgaaa aaataagaag acatataaac tatcccccat gccgcaaaaa aaaactataa
     4381 gtaatgtatt ctttgtggta aatatacctt ttgcaacgta atttttattt atattttcag
     4441 tgtgggtgcc attctcttca ctctcaacac tttcattctc attgtgatta ttgtcgagaa
     4501 cagttatgtt gatttcttca ttgattttga agcctttccg tggcaccgtt ctgataa
//
LOCUS       DAECNO010000194         4551 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.198, whole genome shotgun sequence.
ACCESSION   DAECNO010000194 DAECNO010000000
VERSION     DAECNO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.198"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1225
                     /locus_tag="KKO08_003978"
     CDS             <1..1225
                     /locus_tag="KKO08_003978"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBD6320019.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1225..1899
                     /locus_tag="KKO08_003979"
     CDS             1225..1899
                     /locus_tag="KKO08_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBD6320020.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2427..3188
                     /locus_tag="KKO08_003980"
     CDS             2427..3188
                     /locus_tag="KKO08_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBD6320021.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3232..4311
                     /locus_tag="KKO08_003981"
     CDS             3232..4311
                     /locus_tag="KKO08_003981"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6320022.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4308..>4551
                     /locus_tag="KKO08_003982"
     CDS             4308..>4551
                     /locus_tag="KKO08_003982"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBD6320023.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          865 c         1119 g         1318 t
ORIGIN      
        1 ctttccgtat ggcattttcc agcagcgaaa tgttcttgcg agctggctgg caacaggttg
       61 cggcgtagcg ttgtatctgg ctttaacggc gcgtactcgt tctctgacgt tgatatgttt
      121 gttgtcgtta taccctctgt ctacagcttt agtccttaca caaagccgtg tcggccttct
      181 tggtttattt tgcgtcatcg tgctggccgc tctcgcggac agaccccgct ggcgcggacg
      241 tccccttagc ggtctgttgc gcttgatctt cgtcgcctcc ttgctgtgct ggtgtgtcgg
      301 tatctcaaac tatgccatgc caggggagac aacggctgac tttacgcatt catcatcgaa
      361 tgaccagcgt atacgtattc tgaaaggtgc cataagtctt attcaacagc gccccttaac
      421 cggttggggg ttaggcagct ttgaagcgac cttcccgcaa gccttagagg atgtgggcgt
      481 gacaaacata gagagtgata cggctacgca tccccataac gaactgctgt tcgtaacggc
      541 tgaaggtggc atcgtcgcct tgtttggttt tttgctgcta tcaggcgttt ggctgtggcc
      601 gtgtatatgc cgcctgaaaa tgacaaacaa taacgcgcac catcaatgtg aaaagtggtt
      661 tattgcctta atggggttgc ctttagtcat tcatatgatg accgaatatc ctctctatac
      721 gtctgttccc catttgatgc tgttgctgct ccttttccgc atcggaatgc cggaaaacgt
      781 gatgaatcag gtacgtatac ctaaaacagt ccggttgggt attctgcctg tcattgtgct
      841 cattatgacc gccgcgctgg tcatcctggt agcgggattt gatgcacagc gggaactgac
      901 cagagctgaa gctgatatga acgaaggatt tatccctcgc cttccggaac atagctggaa
      961 atacctgacg caggcagaac gtcttgagcg cgatcagcat ctgcttattg cgaataaacc
     1021 ggggttcgtt cgcgatacgg aaagcatgtc tcttttcact gtgtggggaa ataaatggtt
     1081 ggcaatccac aatgatgcag acgtaagcgc tgcaatgata ttcattgcgc acaggcgcgg
     1141 tgattatctg acagctgaaa aaataagaaa acgcgcagct cgggtatttg taaatgacga
     1201 acgttttact cgcgggggaa actaatggtt aacgatctga cgtataagaa attagaaagc
     1261 acaaacatca actgtaatat tctctcgcca gaaaatacca catggacaaa tgtcacggtg
     1321 attggcctgg atctttttgc catttgcggc gtgtgcagtt tgctcaagcg tgttggggga
     1381 gtgaaacttc aggttttcac ttccttaccc actgatatga atgttctgac tgacagtagc
     1441 atcgtgatat gggtaaaaat gcgtcacgat ggtttgcctg aattagccgg acatattgtg
     1501 aaggtttgtc gtcgataccg tctgatgcgg caactcgtta ttagcgatgc tattcctgaa
     1561 ggaatgacga atgatatcgg gcctctttcc cgagtatggc ttactcatgg gagtgcaaaa
     1621 gtggatattt tattatcttc attacggaag gtgttaaatg ctcattgttt gcctggtcct
     1681 ttgtttataa aacgcctggg aagagtgcaa tggagggttc ttttactccg cgcgaagggg
     1741 attagcacac gtaaaattgc agaaatctgt ggtattggcc tcaagaccgt tagcgcccat
     1801 gagtccgcaa ttcgagaacg attaggcacc aggaataaaa gtgagtacgt ctggttagta
     1861 cgtaatgctc aacttataca acaggcgata cccgcgtagg gaagagatgc gtcaaggtat
     1921 gaaagatgta acaatatgcg aacaaagcaa aaagtataat tgatgcgtca tagataactc
     1981 tctttttcga tgacgaaatc gctgagcatg cgtggtgagg ttagtaatag atgggtgcta
     2041 tgttttgcca cgggtttgta gctgtgaaac ctggaaaaaa ataagaatta aaaatattta
     2101 aagagagtta ataaggttct ggttgtaatt gtcttggtca aaaaaaagat attgtcaatg
     2161 ttgtttttta aattttcagg ttttggttaa atatttgctt acaaatatag gtgatttttt
     2221 tattgtaggc ttttgaaggc tattataact gtgcgctaaa ttaatgcgtt gaataaaaga
     2281 aataacaaaa ttaagaaaag ccatatttac gatatgggca acataaatat aatctaaaaa
     2341 agagtaaggc gttgtaggtt ggttccgcgt tcattacctt gttagtgaac gcggatttat
     2401 cagttgtcga aatggttttt ataataatga aaaattttaa tgtactaacc aaagtcgtat
     2461 tttgcttgtc gtttttatca agcgcatttc ccacctatgc tgcggatgat gtcaaagtga
     2521 ctcaaaacac ttcagttttt aaactgaagc ttagtgcaac gcgattaatc tatgaacaag
     2581 ggtcggatgg cgcagcgata actgtgacaa atccgcagga ttggccgatg cttgtgcaga
     2641 gtaaagtctt tgaagaagat aaagtaactg cagcgccctt tattgtcact ccgccactgt
     2701 tcaggcttaa tccaggccag caaaataaaa tgtgggttgt gcagactgcg ccagctttac
     2761 aagacaggga aaaattacac tggctatgcg ccaccggcat tacgcctaaa aagagtgatg
     2821 tttgggccga gaaagataaa gaaaaaattg ctgaaaatgt gaacctgaac atccaggtta
     2881 acgtacatac ctgcataaaa cttattacca ggccatctgg tattgaaggc aagatggtta
     2941 atgcgagtga gaagttatcg tggaaaaagc agggtgataa aattgtcgcg gtcaatcagt
     3001 cgccgttcta catcaacctt tcttctttac aggttggcgg tagcaagatt gagaaactag
     3061 attatattgc gccgttttcg caacatgagt ttgttatgcc aaaaactagc ggccaggata
     3121 ttaaatggtc tgtgattact gatgaaggtg gtgagagtcg tttgtttata tctcatttgc
     3181 aatcataatg tattacattt ttattgaata tttttatgcc atgcatattc catgttttat
     3241 cgactgtcat taattttgat aatggcctta cttgcgggtc agttaagtgc ccaggagtgg
     3301 tcatttgatt cttcacagtt ggaaggaaac gttagtgcgg acactgtggc aatgtttaat
     3361 cagggagagc aactccccgg aaattatcgt gtggaaattt atcttaacgg tgaaaaagtt
     3421 gatgttgggg agtttccctt tcatcgtccg gaatcccctg aagaaaaaga gcttgttcca
     3481 tgtttaaccg ttgatgacct tatccactat ggcattaaaa ttgataaaag cagcagtgat
     3541 actgacaata aaaagaatca gtgttttaaa tggaactcta ttgaaggttt gaaagtaaat
     3601 tatgattttg atagccagcg cgtacagata accgttccac aactgtatct gcaagataaa
     3661 aaaagtagtt tagcaccagt gtcgttatgg aatgagggtg tagctgcatt ccgcatggtg
     3721 tatcagacaa atattgatat ttccaaacaa aatgataatc aatctaccac gcgtaattct
     3781 cgttatggca ggttcactcc agggtttaat cttggtgcct ggaggtttcg tagttcagtg
     3841 acatggagca aggaacttgg ccagtcagaa agatggcaaa ggggttacat gtggtttgag
     3901 cgaggcataa atgcgattaa aagccgatta acgcttggag aatcttatac gtcgtctgag
     3961 gtgtttgaca gcataccttt tcgcggcggg atgttagcga ccgacgatgc gatgactccg
     4021 ccggaggaca gctactacac acctgtggtg catggaatag cacaaagtga ggcgcaggtt
     4081 attattaaac aaaatgggca aattattttt acccgttctg tgccgccagg gccttttgcg
     4141 ctggataatt taccaacgct ggccgttggc ggggagcttg atgttacagt gagagagagt
     4201 aatggcgagg agcagtattt ttccgtgcca tttcaaacgc cggccatcgc tctgcatgaa
     4261 ggatatttca aatatagtgt gatggggggg aatataaaga agaaggtata aaaagctccc
     4321 ctgtctccgt gggcgaaatc acggcgatgt acgggcttcc ttatggcgtc acggtgtatg
     4381 gagggattca aaacgccact cattttaatg caatatctac gggtattggc atttctctgg
     4441 ggctgttagg gagtttatcg acagatatta cgcgttcgat tgcgaattta tattatggga
     4501 ataaatatcg tattcgttat agtaaatcca ttagtgattt tggtactcaa t
//
LOCUS       DAECNO010000195         4514 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.199, whole genome shotgun sequence.
ACCESSION   DAECNO010000195 DAECNO010000000
VERSION     DAECNO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.199"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..151)
                     /locus_tag="KKO08_003983"
     CDS             complement(<1..151)
                     /locus_tag="KKO08_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6320024.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVW"
     gene            complement(203..541)
                     /locus_tag="KKO08_003984"
     CDS             complement(203..541)
                     /locus_tag="KKO08_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBD6320025.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGG"
     gene            complement(541..2618)
                     /gene="flhA"
                     /locus_tag="KKO08_003985"
                     /pseudo
     CDS             complement(541..2618)
                     /gene="flhA"
                     /locus_tag="KKO08_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2611..3758)
                     /gene="flhB"
                     /locus_tag="KKO08_003986"
                     /pseudo
     CDS             complement(2611..3758)
                     /gene="flhB"
                     /locus_tag="KKO08_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3994..4377
                     /locus_tag="KKO08_003987"
                     /pseudo
     CDS             3994..4377
                     /locus_tag="KKO08_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(4387..>4514)
                     /locus_tag="KKO08_003988"
     CDS             complement(4387..>4514)
                     /locus_tag="KKO08_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6320026.1"
                     /translation="ITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
BASE COUNT         1118 a         1295 c         1104 g          997 t
ORIGIN      
        1 cccagacacg cagagtctcc ggcgtacagc caatctttgg agcaatggaa caaattgtcg
       61 cccattgtga gtcatattcg ccctgacttt ccagaaccat acggactgcc cgttgacgga
      121 cttcggggga aaaacgagta tttttagtca tcctgtttac ctctttctca ggaagtttag
      181 tctccaggat tcccggggcg gttcacccac cgcctggcac ttcccagata aatcgcaacg
      241 gttctgctgc cgctataccg gaaaaggcca cggtggtacc gctctgcccc tctaattcga
      301 cacaacgaga ttgcgaacac aagcgaaccc gcagtcctgc aggtgtcggg ccgataagct
      361 gataacgcca cgccaccagc gtcatcaatc ctgaagcagg ttgtcgcgca gaaagaggtg
      421 cagacgacat cgactcaccg cgatgattta gcgtaattcc gacactactt gcctgccaca
      481 tcccctcccc ggcggcttgc accagcagcg gaaacaataa tatcgctaat aaggttctca
      541 tcatttaccg ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt
      601 cgacaggacc actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcag
      661 cgcgtggttc accaacaata ctggcggcgc acccagcatc tcctgacggg atagagcttc
      721 ctgagtttgc gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag
      781 cgcctgtagt aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt
      841 gccaggaaac cactgctggg taatcgcccg tcccaacgcc acgcgcacga cggcggttaa
      901 ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat
      961 atcgcgaatc ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag
     1021 cgtgacgacg ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc
     1081 caacagctgt tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg
     1141 gttaagatgc gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc
     1201 ctgttctttt agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt
     1261 cgcctcacca ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg
     1321 ataagcatca ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg
     1381 cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa
     1441 tttcttgcgg atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat
     1501 cgggatcagt cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca
     1561 cgtcgcttcg acaacggtat tattctctgc catttttacc ggtttgggtt cggcaggcgc
     1621 tttttgttcg cgtccgcgta tccaccaggc cagccccagc aatccggcag tgaacagcaa
     1681 aaataccagg ttcggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa
     1741 caacataacg cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc
     1801 ggtgctgaca cgcgtaacga tgccccccgc ggcggtagaa atcaccagcg ccggaatttg
     1861 tgccaccaga ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct
     1921 catgccagtt gcagcacgcc gaccagcaac ccgccgacaa cgttaatgac catgatgagg
     1981 atcccggcaa tggcatcgcc gcgaacaaac ttacttgccc cgtccattga gccgtaaaaa
     2041 tcggcttcct gagtcacttc ggagcggcgt tttttcgcct catcttcacc aatcaatcca
     2101 gcgttaaggt cggcgtcaat cgccatctgc ttacccggca taccatcgag aacaaagcgc
     2161 gcacccactt ctgctatacg cccggcacct ttggtaatga ccataaagtt gatgatcacg
     2221 agaatgacaa acaccacgat accgatagcg aaattgccac caacgaggaa gtgaccgaac
     2281 gcttcgacca ccttcccagc cgccgccgcg ccggtatgcc cttccatcaa aatgatacgg
     2341 gttgaagcca cgttaagtgc cagacgcaac aacgtagtaa acaacagaat ggtcggaaac
     2401 gcagcaaact caagcgtgcg ctgggtaaac atcgccacca gcaacaccat gatcgacaag
     2461 gcaatattga aggtaaacaa caggtcgagt atgaatgcgg gcagtggcag caccatcatc
     2521 gacaagatca acaggatcaa aatcggtccg gcaaggatct gccattatgt cgatttcagg
     2581 tttgcgggca ggcgcagcat cgcggccaga ttactcatgg gtcggtttct cgttaataaa
     2641 atccagggct gccggcaccg gaagatgagt aggtgtacag ggcgctgtcc accagccaga
     2701 cgccagcgtt tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga
     2761 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
     2821 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
     2881 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
     2941 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
     3001 cgccgtgcgg cagctcgctg catctgacag atccgtcctt taacatgggg gtcgccttcg
     3061 ccttgtttga actcatcacg aatatcctgc cgcgacattc gcagcttttt caggtggctg
     3121 aagatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
     3181 gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggtga ctcggccatt
     3241 aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg
     3301 atggttttca aaattgcctt aagcaactcc gcgctagtct gtgccgagaa tatccgttta
     3361 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc
     3421 agccccccca gcataaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggt
     3481 agcagcgcca gcatggcttc tctgatcagc agaataattt gcccgaggat cagattcggg
     3541 tctttgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
     3601 gccagcgaca caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag
     3661 gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt
     3721 gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta
     3781 ttaagcgtaa atgatgccag agcgcaaagc gttcaatggt ttaagtaagg ggcaaaacag
     3841 gcgggattta gggcgtttat gcaaaaatag gttcagttgc agaacgtttt gcgaaccatt
     3901 ttccagttct gatatttagc catttagcct atagaatcgc accccgcctc tctgttagtt
     3961 tgctaataat taaattatta caaaggtgat caaatggaat ggcaacaatt gcttaactgc
     4021 aaccgtcgga aagataatct aaaaaaaacc aacatagcat ttttacatcc ccttgctagc
     4081 ggaagacaag agatcgagcg ggattacgac cgattacttt ttgctgcacc aactcgtcgg
     4141 cttgcggata aaacacaagt tttcccgtta gatcaaaatg acagcatacg gacgaggctg
     4201 actcattctc acgaagtagc aaactttgct cgcgggatag gaatgaggct cgctttcgag
     4261 atgaaggagc acatctttgg cgaaataccc acggggattg tcgttgaacg cgacgttcct
     4321 gctctgttag cagcgatcgg actggcacac gatctcggta atccaccatt tgggcatctg
     4381 aagtggtcaa caaaaactgg ccaccgagtt agagtttttc cagtatcgat tttccgattc
     4441 gtttgggggt aacccaccgt tatattcgtg cggtcttagt gcactgtaat atccaacgat
     4501 atagtccgtt atgg
//
LOCUS       DAECNO010000196         4464 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.200, whole genome shotgun sequence.
ACCESSION   DAECNO010000196 DAECNO010000000
VERSION     DAECNO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.200"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(139..1041)
                     /locus_tag="KKO08_003989"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="KKO08_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="KKO08_003990"
     CDS             1101..1379
                     /locus_tag="KKO08_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320027.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KKO08_003991"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="KKO08_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBD6320028.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KKO08_003992"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="KKO08_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBD6320029.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="KKO08_003993"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="KKO08_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBD6320030.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="KKO08_003994"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="KKO08_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBD6320031.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..>4464
                     /locus_tag="KKO08_003995"
     CDS             4371..>4464
                     /locus_tag="KKO08_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="inner membrane protein involved in cell
                     density-dependent acid resistance; part of the acid
                     fitness island (AFI) of E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBD6320032.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1312 a          954 c          943 g         1255 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gctggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggt
//
LOCUS       DAECNO010000197         4324 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.201, whole genome shotgun sequence.
ACCESSION   DAECNO010000197 DAECNO010000000
VERSION     DAECNO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.201"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..158)
                     /locus_tag="KKO08_003996"
     CDS             complement(<1..158)
                     /locus_tag="KKO08_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD6320033.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="KKO08_003997"
     CDS             complement(308..469)
                     /locus_tag="KKO08_003997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320034.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="KKO08_003998"
     CDS             complement(466..654)
                     /locus_tag="KKO08_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBD6320035.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="KKO08_003999"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="KKO08_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBD6320036.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="KKO08_004000"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="KKO08_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="KKO08_004001"
     tRNA            complement(2161..2237)
                     /locus_tag="KKO08_004001"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="KKO08_004002"
     tRNA            complement(2339..2414)
                     /locus_tag="KKO08_004002"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="KKO08_004003"
     CDS             complement(2559..3380)
                     /locus_tag="KKO08_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBD6320037.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="KKO08_004004"
     CDS             complement(3395..3751)
                     /locus_tag="KKO08_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD6320038.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4324)
                     /locus_tag="KKO08_004005"
     CDS             complement(3764..>4324)
                     /locus_tag="KKO08_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD6320039.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1188 a         1179 c          856 g         1101 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaaccaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca
     4141 gcggcgcaag attttttgtg cgctgtttca gtatgctggt ggcggtctgc tctcccggta
     4201 cgatgtcgct ttcacggtac attgagcgga ttttttccgc acgcaacccc agcgaacgac
     4261 gtaataccgc ttccggtagc gcgtccgcca cctgattgcg gaccgcccac caggataatt
     4321 cagc
//
LOCUS       DAECNO010000198         4280 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.202, whole genome shotgun sequence.
ACCESSION   DAECNO010000198 DAECNO010000000
VERSION     DAECNO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.202"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            340..1398
                     /gene="rfaQ"
                     /locus_tag="KKO08_004006"
     CDS             340..1398
                     /gene="rfaQ"
                     /locus_tag="KKO08_004006"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBD6320040.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1395..2519
                     /locus_tag="KKO08_004007"
     CDS             1395..2519
                     /locus_tag="KKO08_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBD6320041.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2512..3309
                     /gene="rfaP"
                     /locus_tag="KKO08_004008"
     CDS             2512..3309
                     /gene="rfaP"
                     /locus_tag="KKO08_004008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBD6320042.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3352..>4280
                     /gene="waaO"
                     /locus_tag="KKO08_004009"
                     /gene_synonym="rfaI"
     CDS             3352..>4280
                     /gene="waaO"
                     /locus_tag="KKO08_004009"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBD6320043.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCA"
BASE COUNT         1197 a          870 c          983 g         1230 t
ORIGIN      
        1 ttttgactta aatagcaatt tacccgccaa ttcttgctga acatctatac tttttttgta
       61 tttttatgcg attcattgat ttctgctgta cgtgcaggaa ggaatcgcag agcaacagtg
      121 aaagaatatg gttgctaatg attttatttg attgatttct ttattgaaaa tcaacaatgt
      181 gcttaaaaat cattcgggtc ggattgccgc gaaagaagtg atacactagc gcgtcaatat
      241 cagtgcggta tcagtattca ggtagctgtt gagcctgggg cggtagcgtg ctttttctgc
      301 ttcaccttaa tcggataatc tcaacaaaaa gagttacttg tggataagcc atttcgaaga
      361 attttgctca ttaagatgcg ttttcatggg gatatgttat taactactcc cgtcattagt
      421 tctctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc tgctttatca ggacaccatc
      481 ccgatcctgt ctgaaaatcc agagattaac gcgctctacg gcataaaaaa taaaaaagca
      541 aaagcctcag aaaaaattgc caactttttt catctcatca aggtattacg tgccaataag
      601 tatgacctta tcgtcaatct taccgatcaa tggatggttg ctatactggt tcgcttatta
      661 aatgctcgtg tgaaaatttc ccaggattat catcatcggc agtctgcttt ttggcgtaaa
      721 agtttcaccc atttggtgcc gttgcagggt ggaaatgtgg tggaaagtaa cttatccgtg
      781 ctgacaccat tgggacttga ttcgttggtg aagcagacaa ccatgagtta cccgcctgca
      841 agctggaaac gtatgcgtcg cgaacttgat cacgctggtg ttggacaaaa ttatgtggtt
      901 atccaaccta cggcgcggca aatcttcaaa tgctgggaca acgccaagtt ttccgctgtg
      961 attgatgcct tacatgctcg tggttatgaa gtcgttctga cgtccggccc agataaagac
     1021 gatctggcct gcgtcaatga aattgcgcag ggatgccaga cgccaccagt aacggcgctg
     1081 gctggaaagg tgaccttccc ggaacttggt gcgttaatcg atcatgcgca gctgtttatt
     1141 ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta atacgccgct gatatcgctg
     1201 tttggcgcaa cagatcatat tttctggcgt ccctggtcaa ataacatgat tcaattctgg
     1261 gcgggagatt accgggaaat gccaacgcgc gatcagcgtg accgaaatga gatgtatctt
     1321 tcggttattc cggcggcaga tgtcattgct gctgtcgata aattactgcc ctcctccacg
     1381 acaggtacgt cgttatgatc gttgcttttt gtttatataa atattttccc tttggcggtt
     1441 tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc ccgaggtcat catgttcggg
     1501 tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt tgaagtgatc aaagtgccgg
     1561 ttaaatcgca taccaatcac gggcgcaatg cggagtattt tgcctgggtg caaaaacatt
     1621 tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa aatgccgggg ctggacgttt
     1681 attatgccgc tgatgtttgt tatgccgaga aagtagcgca ggaaaaaggc tttttctatc
     1741 gcctgacgtc acgttatcgc cattatgccg cctttgagcg ggcaaccttc gaacagggca
     1801 agccgacaca gctgctgatg ctgacagata agcaaatcgc cgatttccag aaacattatc
     1861 agactgaagc ggagcgtttt catattctgc caccggggat ttatcctgat cgtaaatata
     1921 gccagcagcc agccaatagc cgtgaaatct tccgtaagaa gaatggaata accgaacaac
     1981 aatatttatt gttgcaggtc ggttcagact tcacgcgtaa aggtgccgat cgttccattg
     2041 aagcacttgc ttcgttaccg gattcgctgc gccacaacac attgctatat gttgttgggc
     2101 aggataaacc gcgaaaattt gaggcactgg cagaaaaacg cggcgtgcgc agtaatgttc
     2161 acttcttctc ggggcgcaac gatgtctcgg aattaatggc ggcggcggat ttattactgc
     2221 atcctgccta ccaggaagcg gcgggaattg tgctgctgga agcgattact gcaggattac
     2281 cggtactaac aactgccgtt tgtggctatg cgcattatat tgtcgacgct aattgcggcg
     2341 aggctattgc tgagccattc cgccaggaaa cattgaatga gattttacgc aaagcgttaa
     2401 cgcaatcttc attgcgccag gcttgggcgg aaaatgcgcg acattatgct gatacacaag
     2461 atttatacag tctgccagag aaagcggcgg atatcataac gggtggtctg gatggttgaa
     2521 cttaaagagc cgtttgccac gttatggcgc ggcaaagatc cttttgagga agttaaaacc
     2581 ttgcagggtg aggtatttcg tgaactggaa actcgccgta ctctgcgctt tgaaatggcg
     2641 ggaaaaagct attttctcaa atggcatcgc ggcacgaccc tgaaagagat aatcaaaaat
     2701 ttactctcat tgcggatgcc agtattaggc gcagaccgcg aatggaatgc gattcatcga
     2761 ctgcgggatg tcggcgttga tactatgtat ggggtggcat tcggcgaaaa aggcatgaat
     2821 ccgctgacca gaacctcgtt tattattacc gaagatctga caccaaccat cagtctggaa
     2881 gattactgtg ctgactgggc gaccaaccca ccagatgttc gcgtaaagcg tatgcttatt
     2941 aagcgtgtcg cgacgatggt gcgcgatatg catgctgcgg gcattaacca tcgtgactgt
     3001 tatatctgtc atttcctgct gcacttgcct ttttccggta aggaagagga gttaaaaatt
     3061 tcggtaattg acctacaccg ggcgcagctt cgcacgcgcg ttccaggtcg ttggcgcgat
     3121 aaagatctta tagggcttta tttttcttca atgaatattg gcctgactca gcgggatatc
     3181 tggcgcttta tgaaggtgta ttttgccgcc ccgcttaaag acattctcaa gcaggaacaa
     3241 ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca gggaaagaac gattcgaaaa
     3301 tcgttgtaac ccgtaactta tttttgccaa aattttggat acagaataaa tatgtctcaa
     3361 ctcaatgata gtgacatcat cctttttgag tataattttc attatcaaaa tataagatct
     3421 aaaaatactc ttgatatagc atttggtatt gacagaaatt ttctttttgg atgtggtgta
     3481 gccatcgcat ctattctatt aaacaataga gaaatctctt gtgaatttca tgttttcaca
     3541 gattatatca gtgataaaga caaattatat ttttctgatt tagcaaaaca atataattca
     3601 agaattaata tttatgttat caattgtgat aagctgaagt cattaccaag cacgaaaaac
     3661 tggacttacg caacatattt tcgatttata attgcagatt atttctatca taaacatgaa
     3721 aaaatactat atcttgatgc agatattgct tgcaagggta gtattaaaga actcttagat
     3781 tatcaatttt ctactaatga aattgccgca gttgtagctg aaagagatat tgaatggtgg
     3841 caaaatcgag cctcggtatt aactacacca cagttagctt ctggatattt caatgctggt
     3901 tttttactga taaatattga tgagtggaat ctaaataaca tttcgtcaaa agctattgaa
     3961 atgttgcgtg acccagattg ggtaagtaaa atcacccacc ttgatcaaga tgtactgaat
     4021 gtattattga atggtaaagt gaagtttatt tcggagaaat ataatacccg atatagtatt
     4081 aactatgaat taaaagacaa agttgataat ccagtcaatg atgacaccgt gtttatacac
     4141 tatgtgggac ctacaaaacc ctggcatgag tgggctgatt atccggtttc acgtagtttt
     4201 ttgatcgcca aagcagcttc tccgtggagt aaagaagatt tacttaaacc tgtaaatagc
     4261 aatcagtatc ggtattgtgc
//
LOCUS       DAECNO010000199         4248 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.203, whole genome shotgun sequence.
ACCESSION   DAECNO010000199 DAECNO010000000
VERSION     DAECNO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.203"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            35..916
                     /gene="yddG"
                     /locus_tag="KKO08_004010"
     CDS             35..916
                     /gene="yddG"
                     /locus_tag="KKO08_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBD6320044.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1171..2271
                     /gene="ompC"
                     /locus_tag="KKO08_004011"
     CDS             1171..2271
                     /gene="ompC"
                     /locus_tag="KKO08_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBD6320045.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2435..2920
                     /locus_tag="KKO08_004012"
     CDS             2435..2920
                     /locus_tag="KKO08_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD6320046.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3030..4019
                     /locus_tag="KKO08_004013"
     CDS             3030..4019
                     /locus_tag="KKO08_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBD6320047.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1318 a          875 c          805 g         1250 t
ORIGIN      
        1 aatttttaac ggcaagagag acaaaacagc gagcatgaca cgacaaaaag caacgctcat
       61 agggctaata gcgatcgtcc tgtggagcac gatggtagga ttgattcgcg gtgtcagcga
      121 ggggctcggc ccggtcggcg gcgcagctgc tatctattca ttaagcgggc tgctgttaat
      181 cttcacggtt ggatttccgc gtattcggca gattccgaaa ggctatttac tcgccgggag
      241 tctgttattc gtcagctatg aaatctgtct ggcgctttcc ttagggtatg cggcgacccg
      301 tcatcaggcg attgaagtgg gtatggtgaa ctatctgtgg cccagcctga caattctctt
      361 tgccattctg tttaatggtc agaaaaccaa ctggctgatt gtaccaggat tattattagc
      421 cctggtcggc gtctgctggg tgttaggcgg tgagaatggg ttacattatg atgaaatcat
      481 caataatatc accaccagcc cattgagtta tttcctggcg tttattggtg cgtttatctg
      541 ggcagcctat tgcacagtaa cgaataaata cgcacgcgga tttaacggaa ttaccgtttt
      601 tgtcctgcta acgggagcat gtctgtggat ttactatttt ctcacgccac aaccagaaat
      661 ggtatttagc acgcccgtca tgattaaact catctctgcg gcatttacct taggatttgc
      721 ttatactgca tggaatgtcg gtatattgca tggcaatgtc accataatgg cggtaggctc
      781 gtattttacg cctgtacttt cctcagcgct tgcagccgtg ctgctcagcg ccccgctgtc
      841 gttctcgttc tggcaaggcg cactaatggt ctgcggcggt tccctgctct gctggctggc
      901 gacacgtcgt ggttaaaata attgccgggt ctacggcccg gcagttaatc agataaattc
      961 cgacaatatt aaaaaataaa tttcaaatac cagtaattca ccttatatct catttcgact
     1021 tcgatcacat atcgataaat aatattattc tatttttctc attgttttta atcaccattc
     1081 tattttaaaa acagtatgtc atttataaat gacgccgttt aaaattcgtt tagaaaatac
     1141 gtttaacgat aattaaaagg attctgttaa atgaaattaa aaatagttgc ggtggttgta
     1201 actggtttgt tagctgcgaa cgtagcacac gctgccgaag tctataacaa ggatggtaat
     1261 aaactcgacc tttatggcaa ggttaccgct ctacgttatt ttactgatga taagcgtgac
     1321 gatggtgata aaacttatgc ccgtctcggc tttaaaggag aaacgcaaat caatgatcaa
     1381 atgattggtt ttggtcactg ggaatatgat tttaaaggct ataacgatga agccaacggc
     1441 tcgcgcggca acaagacccg tctggcctat gctggtttaa aaattagtga atttggctct
     1501 ctggactatg gtcgtaacta cggtgtcggc tacgacattg gttcatggac cgatatgttg
     1561 ccagaatttg gtggcgatac ctggagtcag aaagatgtct tcatgacata ccgtaccacc
     1621 ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa ttgaagggct gaactttgcc
     1681 gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc atctttatgg tgctgactac
     1741 acgcgtgcca acggtgacgg tttcggtatc tcctcaactt atgtttatga tggctttggt
     1801 atcggtgcgg tatataccaa atccgatcgg acaaatgcgc aggaaagagc cgcagctaat
     1861 cctctcaatg cctccggtaa gaatgcagaa ctatgggcca caggtataaa gtatgatgcc
     1921 aacaacatct actttgcagc taattacgat gaaacattaa acatgaccac ctatggggat
     1981 ggttatatct ctaacaaagc acaaagtttt gaagtggtgg cgcaatatca attcgacttc
     2041 ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca gagatctggg ccgctacgcc
     2101 gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt atttcttcaa caaaaatatg
     2161 tcgacctatg ttgattataa aatcaacctg attgatgaaa gcgactttac ccgtgccgta
     2221 gatattcgca ccgataacat cgtcgctacg ggcattacct atcagttcta acgtcctctc
     2281 ctgaccagcc cttctctggg ctggtttttg atttaagtgc tgctgtatga ctccttaagt
     2341 aaaaatgagc aattcttgat atttctggtc attcacctca attaatatta ttccatatcc
     2401 tccggggaca tcttactata atatttattg atgagtgatt atgaccaata ttaatacagc
     2461 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
     2521 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
     2581 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
     2641 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
     2701 aaatctggaa aacaattgtc taacttgtct tgacttagct gacaatgcca gtctcgttaa
     2761 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
     2821 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
     2881 ggttaatctg gaagcgcaaa ataatagcct aaaaaaatga tatttctgat agttataaac
     2941 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
     3001 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
     3061 ccaggatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
     3121 ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
     3181 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
     3241 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
     3301 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
     3361 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
     3421 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     3481 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     3541 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     3601 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     3661 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     3721 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     3781 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     3841 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     3901 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     3961 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgac
     4021 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     4081 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gcggataacg
     4141 aattgtttca ctatactatt aaaaaagcat ataccccata taatatacac acttacaaga
     4201 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacct
//
LOCUS       DAECNO010000200         4110 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.204, whole genome shotgun sequence.
ACCESSION   DAECNO010000200 DAECNO010000000
VERSION     DAECNO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.204"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(954..1145)
                     /locus_tag="KKO08_004014"
     CDS             complement(954..1145)
                     /locus_tag="KKO08_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBD6320048.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            1209..1532
                     /locus_tag="KKO08_004015"
     CDS             1209..1532
                     /locus_tag="KKO08_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBD6320049.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1522..1833
                     /locus_tag="KKO08_004016"
     CDS             1522..1833
                     /locus_tag="KKO08_004016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320050.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            1808..2230
                     /locus_tag="KKO08_004017"
                     /pseudo
     CDS             1808..2230
                     /locus_tag="KKO08_004017"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2211..2741
                     /locus_tag="KKO08_004018"
                     /pseudo
     CDS             2211..2741
                     /locus_tag="KKO08_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
     gene            2748..2981
                     /locus_tag="KKO08_004019"
     CDS             2748..2981
                     /locus_tag="KKO08_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBD6320051.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3527..3994)
                     /locus_tag="KKO08_004020"
     CDS             complement(3527..3994)
                     /locus_tag="KKO08_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320052.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT         1000 a          938 c         1194 g          978 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtccctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggtatt tcatacccgg tatggcgcac ttttcataca
      781 atctgcactg atgccgcatg gttaagcccg tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaaaac ctgctgatgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga gcggatgccg ggagcagcat atgtcagagg
      961 ttttcaccgt catccccgaa acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     1021 tgaaacgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttcagga ggtaatgata ccgatgaaac
     1201 gcgagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgct
     1261 gtgagggtaa acagctggcg gtatggatgc ggcgggtctg cctgggggag ccggttgccc
     1321 gttccgggaa actgccgaca ctggcaccgc cgttactgtg tcagctggct gccatcggga
     1381 ataacctgaa tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtaaccggg
     1441 ttcaggtggt ggccgcactg atggccatcg gggacgagct gcgccggctg cgtctggccg
     1501 tcagggaaca ggggacgcgg gatgatagtt aagtttcatg cccggggaaa aggtggtggc
     1561 agtggtccgg ttgattacct gctgggaagg gagcgtaacc gggaaggggc aacggtgctt
     1621 cagggtaatc cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac
     1681 acgtccggtg ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg
     1741 atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa aaaatcagta cagcatcctg
     1801 tgggtggatg caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     1861 gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc ctagaattga
     1921 tgcctggcag acgctggtta atcactatta cgggctgcat gacccgaacg ccccggagaa
     1981 ccgcaggacg ctgacactcc ctgataatct gccggaaacg aaacaggcga ttgctgaggg
     2041 cgttacacgc gggatagacg cgctttacca tgccggagag ataaaaggcc gtcaggatgt
     2101 gattcaggct ctcacagagg cagggattga cgtggttagg gtgacgcgaa gcagtatcag
     2161 cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcgtttt atgagcaatc
     2221 ttttacagac gggtgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     2281 aaatgctgaa cgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
     2341 acgaatcttt aatcaggaac gctatcaacg accatacatc agccctgaaa gaacttctgg
     2401 agaggtatca gaaaacgacc gtcgatacga tggacgcaca ctggaaaacc gtactgaaaa
     2461 tgagcgtgaa acgctggctg tggctgatta tcgtctcagt actgatgttt gccacgaccg
     2521 ggagcctgct gtggtaccag ggcatgaaga taaacgcgaa catgaatatt ctccgcgagt
     2581 agaaagagag tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgaggaca
     2641 gcaacggacg ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg
     2701 aaaacgggaa acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg
     2761 aaagacattt gctgaacgca ttagaacaac tgcaacagga ctatatgcag cggctgaacg
     2821 aatgggagag cgccttcgtg gaattgcaga aaatgttttc gcttacgcaa cgggacaacg
     2881 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     2941 cagaacgctt gagccagtta tacagcgaga acaggagata agggacgaac ggctgataca
     3001 ggaatgcgaa catgcgttat ccctggagcg tgagcaccag ctggaaatac aggaacggac
     3061 actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg tcagcgcgtt
     3121 tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg cttcagtcag
     3181 accttgctga aaacatagga acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt
     3241 ttgttgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac ttcttttttc
     3301 ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc
     3361 tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt ggttaaaatt
     3421 taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg
     3481 ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac gttaaattgt
     3541 gagggaatgg tccccatata ctgactttat catagggata gggttggtag tgggtcgatt
     3601 gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg caacagaagg
     3661 cgtgagattc tcctgggcct ggaaaggata agtgtttaga attgatggca aaatatcttc
     3721 catccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgttg ttacaaactt
     3781 cttgaatttg tagtttttgc aggactctta ttactgcgtt atcatgttcc aattctttag
     3841 gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt tgttgtctaa
     3901 agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg tttggcgggg
     3961 tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt atagcatcaa
     4021 caaatacggc tataaaacaa aatttttata gagtgattca agatcttctt gagatccttt
     4081 tttgcggcgc gtaatctgct gcctgcaaac
//
LOCUS       DAECNO010000201         3714 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.205, whole genome shotgun sequence.
ACCESSION   DAECNO010000201 DAECNO010000000
VERSION     DAECNO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.205"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="KKO08_004021"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="KKO08_004021"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBD6320053.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="KKO08_004022"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="KKO08_004022"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBD6320054.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2657..2732
                     /locus_tag="KKO08_004023"
     tRNA            2657..2732
                     /locus_tag="KKO08_004023"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Thr,seq:cgt)
     gene            complement(2747..>3714)
                     /locus_tag="KKO08_004024"
     CDS             complement(2747..>3714)
                     /locus_tag="KKO08_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6320055.1"
                     /translation="LEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKM
                     TNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLD
                     KAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQ
                     IDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTK
                     APTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWES
                     IEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          853 a          934 c          993 g          934 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc
      361 cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc
      481 tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag
      601 agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg cgcagaaatc
      721 cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac taaattgcag gccgctgacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc
     1441 tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc
     1681 gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa cctgtcgcac
     1741 taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct taccggcggg
     1801 tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga
     1921 acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga
     1981 aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag
     2041 cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcgct
     2161 gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc
     2281 ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
     2461 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa
     2701 ggtcgtaggt tcgactccta ttatcggcac cattaaaatc aataagttac acatcattag
     2761 taccttcctt attttttgac tgggacaaat ttgggaccga tgggttcagg atcgagtcta
     2821 tttgccgtgc gtgttcggta aggtgattag gtgcaaggtg agcatatcga cgaaccattt
     2881 cgatagactc ccagcctccc atttcctgta acactgacaa cgggactccg gcttgaacca
     2941 gccaacttgc ccaggtgtgt ctcaagtcgt gaaatctgaa atcatcaata ccagcccgtc
     3001 tcagcgccgc tttccaggct gtgtttgcgt cataccgcat cttccttact gttggcgctt
     3061 tcgttccgtc tggtttggta cagctttcct tgtacacaaa tacccaacgg tgatgattcc
     3121 cgatttgttt tttcaatacg cgacatgcag tatcattcag cgcaacgcca attgcgcggt
     3181 ttgatttact ctcttccggg tttatccatg ccacccggcg ctgcatatct atttgttgcc
     3241 attcaaggtt gatgatgttc gagcgtctta agcctgttgc cagtgcaaat tcaacaacag
     3301 actttaatgg ctccggacat tcatcaatca gcctttgtgc ttcatggggc tccagccagc
     3361 ggatccgttt attctttggt tgaggcactt taataattgg tgccttatcc agcattttcc
     3421 attcacgctc tgcggctctt agtagggcct ttataaatga aagatgcgta gccttcgttg
     3481 caacggacgc tggttttggc gtgtattctg gaacaggttt cccttttttt ctgcatgctt
     3541 ctgccctgag tttccagttt tcctcatgac gccggttcgt cattttctgc attgctgaat
     3601 aaatttttga ttcagtgatg tctcttagtt gcatccctgc gaaatgttga agccagaatc
     3661 cgatccggct tttgtcatcg tccagtgatt tcttatgtgc tttctcttcg agcc
//
LOCUS       DAECNO010000202         3590 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.206, whole genome shotgun sequence.
ACCESSION   DAECNO010000202 DAECNO010000000
VERSION     DAECNO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.206"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            32..493
                     /locus_tag="KKO08_004025"
     CDS             32..493
                     /locus_tag="KKO08_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBD6320056.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="KKO08_004026"
     CDS             complement(534..1004)
                     /locus_tag="KKO08_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBD6320057.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KKO08_004027"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="KKO08_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBD6320058.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KKO08_004028"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="KKO08_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBD6320059.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          926 c          817 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DAECNO010000203         3548 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.207, whole genome shotgun sequence.
ACCESSION   DAECNO010000203 DAECNO010000000
VERSION     DAECNO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.207"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="KKO08_004029"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="KKO08_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBD6320060.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="KKO08_004030"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="KKO08_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBD6320061.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KKO08_004031"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="KKO08_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBD6320062.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAECNO010000204         3411 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.208, whole genome shotgun sequence.
ACCESSION   DAECNO010000204 DAECNO010000000
VERSION     DAECNO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.208"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(226..429)
                     /locus_tag="KKO08_004032"
     CDS             complement(226..429)
                     /locus_tag="KKO08_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320063.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="KKO08_004033"
     CDS             complement(431..586)
                     /locus_tag="KKO08_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBD6320064.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="KKO08_004034"
     CDS             complement(789..1196)
                     /locus_tag="KKO08_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6320065.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="KKO08_004035"
     CDS             1273..1500
                     /locus_tag="KKO08_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6320066.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="KKO08_004036"
     CDS             1484..1906
                     /locus_tag="KKO08_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320067.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2767
                     /locus_tag="KKO08_004037"
                     /pseudo
     CDS             1984..2767
                     /locus_tag="KKO08_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2774..3411
                     /locus_tag="KKO08_004038"
                     /pseudo
     CDS             2774..3411
                     /locus_tag="KKO08_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          882 a          750 c          918 g          861 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagattttta tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gttggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagaggaaga tctaaatact gatctccccc taaatccccc tcgccaaaaa
     2461 cgagcgtcta aaaaattcga gccggaggct attgagctgc ccgattggtt gccggaaaca
     2521 ctctggcatg agtgggtccg gttcagacag gcattgcgaa aaccgattcg aacggagcag
     2581 ggcgatacgg gaactggaaa aattccgtca gcagggtttt acacctgagc aggtgattcg
     2641 acacagcatc gccaatgaat accagggcct gttcgcgccg aaaggtgttc ggcctgagac
     2701 gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc caggcttcag
     2761 ggggtaacgg accatgaaaa atattgcgac aggcggcgtt ctggaacgca tccgccgact
     2821 gaccccgcaa catgtaaccg ccccattcag aacggtagcg gagtggcgcg agtggcaact
     2881 tgctgaaggc cagaaacgta gcgaggagat caaccgcctg aatcgccagt tgcgggtgga
     2941 aaaaattctg aatcgctcag gcatccagcc gttgcaccgt aaatgctcgt ttgcgaatta
     3001 ccaggtgcag aacgacggcc agcgatacgc gttaagccag gcgaaatcca tcgccgatga
     3061 actgatgacc gggtgtacaa attttgcgtt cagcggaaaa cctgataccg ggtagaatca
     3121 cttagcggca gctatcggga atcgcctgct gaaagacggt cagacagtga ttgtggttac
     3181 cgtggctgat gttatgagcg ccctgcacgc cagctatgac gatgggcagt caggcgaaaa
     3241 atttttgcgg gagctgtgcg aagtggatct gctggttctt gatgaaattg gcattcagcg
     3301 cgagacgaaa aacgagcagg tggtgctgca ccagattgtt gatcgccgga cagcgtcgat
     3361 gcgcagcgtg gggatgctga caaacctgaa ctatgaggtg atgaaaacat t
//
LOCUS       DAECNO010000205         3251 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.209, whole genome shotgun sequence.
ACCESSION   DAECNO010000205 DAECNO010000000
VERSION     DAECNO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.209"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="KKO08_004039"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="KKO08_004039"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBD6320068.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="KKO08_004040"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="KKO08_004040"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBD6320069.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1132..1890
                     /gene="tam"
                     /locus_tag="KKO08_004041"
     CDS             1132..1890
                     /gene="tam"
                     /locus_tag="KKO08_004041"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBD6320070.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1894..2808)
                     /locus_tag="KKO08_004042"
     CDS             complement(1894..2808)
                     /locus_tag="KKO08_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBD6320071.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            3009..3197
                     /locus_tag="KKO08_004043"
     CDS             3009..3197
                     /locus_tag="KKO08_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320072.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT          836 a          778 c          858 g          779 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactac cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aatgtctgac
     1141 tggaacccct ctttatatct gcacttttcc gctgaacgat cgcgtccggc ggtggagctg
     1201 cttgccagag tgccgctgga gaatgtcgaa tatgttgccg atcttggctg tggcccaggt
     1261 aacagcaccg ccctgctgca tcaacgttgg cctgcggcca ggataacagg catcgactcg
     1321 tctccggcga tgattgctga agctcgcagt gctttgccag actgccagtt cgtggaagca
     1381 gacatccgca actggcaacc ggaacaggca ctcgatctga tttttgctaa tgcctcactg
     1441 caatggctgc ccgaccacta cgaattgttt cctcacttgg tttctttact taatccgcag
     1501 ggcgtgctgg cagtacagat gccagataac tggctggagc cgacccatgt gctcatgcgc
     1561 gaagttgctt gggaacaaaa ctacccggat cgcgggcgtg agccgttggc tggcgttcat
     1621 gcttactacg atattttgag cgaagccgga tgtgaggtcg atatctggcg aaccacttac
     1681 tatcaccaga tgccgtcgca ccaggcgatt atcgattggg tgaccgccac tggattacgt
     1741 ccgtggttac aggatctgac cgagagcgaa cagcagcttt ttcttacgcg ctaccatcag
     1801 atgctggaag agcagtatcc actgcaagag aacggacaga tactgctggc atttccgcgt
     1861 ctgtttattg ttgcccgccg tacggagtaa ttatcacgtc agctggtaat gacgatcggg
     1921 aagaattttt gctggaattt ctgcttcatc gttcatctgt aacaggtcaa tttcaatagc
     1981 gttgcagatg gcatccagcg gtaaatcatt gttttcagta ccgaacggtt cttccagttc
     2041 ttccgccaga caatccagcg aaataaaagt gtaggaaatc agcacagaga taaaaggcgt
     2101 catgtaatgc aggtccacga ccagcgcgaa cggcagcatg atacaaaaca gataaacggt
     2161 acgatgcaaa atcagggtgt aggcaaaggg aattggcgtg taggcaatgc gctcacagcc
     2221 tgccagaacc gctgaaatat cattaagccg atcgttgagg ctaataaaca ggatatctga
     2281 aagctgtcca ttgcggcgct gaaccgccaa ccattctccc attattaaca agatacggtt
     2341 agctggagag ttcgaagcca gtacacgctg aagatctcca gtcttgagat aatgagccag
     2401 cacttccgcc tgtggctgtt tgcgtaatgt catgcgtaaa cagtgggcga aggcgatttg
     2461 cagcctggca aactccctta cacttgccga atccggcaat gtcgttttta cctctcgcag
     2521 taacgaccgt gaggcaatca tcaactgccc ccaaagtttt cgcgcttcaa cgtaacgggc
     2581 gtacccggca ttattacgaa aaccaagaaa aatggcgatg gcgacaccga gaatgctgaa
     2641 cggtgcgagg gtgaatttga tgcccagatg cgtgtaccag ggcagcatga atataacagc
     2701 aatagaaaag agaaaattga gtagtaagcg cgaggatatc ttggataata ctgagccgtg
     2761 ccagacaaaa atacggcgca gccagtgttg ttgtggacga acaatcatgg ttatcttcag
     2821 gcgtggaaaa gtcgccctat taaacgtgat tacgatcaca ttctcaagac gctacttaca
     2881 aattacctag aaccacggca gcctgacggc tgcaaattga aaactgcccc tgttcgtcct
     2941 gttgttgccg cacgctccgc agaatatgta tgctctgagg acctgtttta ttaaggactc
     3001 atcctcttat gttgacccgc aaaagtatag ataccgttct gctctctgtt ggtgctgaga
     3061 agctctcaca acgtgaatgg gactggatga agatgctcaa acccatggac ccaccacctg
     3121 cgatggtcgc ggcctccatc ctggagcgcc gtggcgacac agcagccctt acccggctac
     3181 aggataccgg gggttaatcc gtcctcatgt tgtccgcttt tactccccga ccgctgaaac
     3241 gcctgttcac a
//
LOCUS       DAECNO010000206         3202 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.210, whole genome shotgun sequence.
ACCESSION   DAECNO010000206 DAECNO010000000
VERSION     DAECNO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.210"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(40..666)
                     /gene="dsbA"
                     /locus_tag="KKO08_004044"
     CDS             complement(40..666)
                     /gene="dsbA"
                     /locus_tag="KKO08_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBD6320073.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(683..1669)
                     /gene="srkA"
                     /locus_tag="KKO08_004045"
     CDS             complement(683..1669)
                     /gene="srkA"
                     /locus_tag="KKO08_004045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBD6320074.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1746..2015)
                     /locus_tag="KKO08_004046"
     CDS             complement(1746..2015)
                     /locus_tag="KKO08_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBD6320075.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2085..2669
                     /gene="mobA"
                     /locus_tag="KKO08_004047"
     CDS             2085..2669
                     /gene="mobA"
                     /locus_tag="KKO08_004047"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBD6320076.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2651..3178
                     /gene="mobB"
                     /locus_tag="KKO08_004048"
     CDS             2651..3178
                     /gene="mobB"
                     /locus_tag="KKO08_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBD6320077.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          839 a          779 c          769 g          815 t
ORIGIN      
        1 cccgtgaata ttcacgggct ttatgtaatt tacattgaat tatttttcct cggacagata
       61 tttcaccgta tcagcatact gctgaacaaa aacatccata ttgctggtat ccataccctg
      121 cggattcagc tgatatttac cgttaacaaa catcgccgga acaccacgca attgcacatc
      181 agctgcggct ttttcctgct gagcgaccag agatttcacc acgaagctgt tccacgccgc
      241 gtcgtactct tcacctttaa tacctgcatt gataaataca tcgcggatat cagatgctga
      301 acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt tgtcttccac
      361 gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt caccacccat
      421 gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct tcacattatc
      481 agaaatatgc agaacttctt caaactgata gcagtgcggg cagaagaaag agaaaaactc
      541 cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt taccatcttc
      601 atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca gccaaatctt
      661 tttcatgatc tactctctcc gattaataca taggtgttaa ttgcaaaggg ggttcttgta
      721 gaacttttgc ctgttctata aaagtcgccg tctgtcgtag ccagtaatct tccccggtta
      781 accacgggaa atttttcggg aacgcgggat cagcccaatg ccgcattagc caggcaagat
      841 aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact
      901 cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc tgggctttat
      961 cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca tcatccagat
     1021 caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct ccatgtagcc
     1081 gcagaacggt gaaatcttcc cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg
     1141 ctttcagaaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca aacagcttgc
     1201 gaggctcaat gagatattca ttcagaccga tggtcgggcg atgaataaaa agctgtttgc
     1261 gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga tcgatattat
     1321 cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat
     1381 tcaataaagt ctgaccgtta aaggccacag gtgctgcgac cggaacttca tcatttacca
     1441 gctgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt tcagggcgat
     1501 aaaatttgac gacaaaacgt cgacgctctt cgtcctgaaa ttgatagaca cggttttcat
     1561 agctgttaag cggggtaaga ccggaatcca cccggatccc ttgctcaaac agagcgtcca
     1621 tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc ccatcatccg
     1681 gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc gccgcttaca
     1741 agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca
     1801 taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat tttcaggtga
     1861 tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga ctctttcgcc
     1921 agtttctgca aaaattgcaa caagttaaag tctggctctt tttgccaggc tggctggagg
     1981 agttcaataa cttcattcag acgtttacat ttcatagtaa tgctccttac tcttgagaca
     2041 gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat ctgatgacga
     2101 cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggcggc gtagataaag
     2161 gattgctgga cttaaatggc aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc
     2221 agctctctca cgtcgtggtt aatgctaatc gtcatcagga aatctatcag gcaggcggtc
     2281 tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcagga atgctttcag
     2341 taatgcagca ggaagctggt gagtggtttt tgttttgtcc gtgcgatacg ccttacattc
     2401 cccatgattt agccgcccgg cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc
     2461 atgacggtga acgcgatcac ccgactattg ctctggtaaa ccgcgctatt gagcctttat
     2521 tactggaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt ctggctggcg
     2581 gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat acgccagagg
     2641 agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg cgtggagtgg
     2701 taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatgcgcca gagggatccg
     2761 tccggggctg attaagcata cgcaccatga tatggatgtt gataagccag gcaaagatag
     2821 ctatgagctg cgcaaggctg gcgcggcaca aaccatcgtt gccagccagc aacgatgggc
     2881 cttgatgacg gaaacaccag acgaagaaga gctggatcta cattttctcg caagtcgaat
     2941 ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag agatcgcaaa
     3001 gattgtgctg tttcgcgatg gagccggaca tcgacctgaa gaattagtga tagacaggca
     3061 tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac tggatattaa
     3121 tgatgttgag gggctggctg attttgtggt ggagtggatg caaaagcaga acggataagc
     3181 aagagtaggc tggatcagac gc
//
LOCUS       DAECNO010000207         3177 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.211, whole genome shotgun sequence.
ACCESSION   DAECNO010000207 DAECNO010000000
VERSION     DAECNO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.211"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            417..1526
                     /gene="fepE"
                     /locus_tag="KKO08_004049"
     CDS             417..1526
                     /gene="fepE"
                     /locus_tag="KKO08_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBD6320078.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2551..2745)
                     /locus_tag="KKO08_004050"
                     /pseudo
     CDS             complement(2551..2745)
                     /locus_tag="KKO08_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          658 c          773 g          945 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgccctggg ggcgctgcta ggggtgtctg ttcagatatg tagcaatgag tcagggattc
      121 acgaactgaa aatgtccgaa aatgtgtctc atgggcactg tatgcgccca aaccgatcgt
      181 cattcacttt catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg
      241 tttaccccgg ggaaaacatc tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt
      301 tttagcggtg cgcgcaataa aatgttatgt taaatgtctg ctgaatggtt tttaggtttt
      361 ttcctaacat atgatagaat aaaaaaacca tatgttaata tattgtgttt ttttcaatga
      421 aaatctcaaa ggtctctgac gaacattttt ctaattacaa catgttgccg aatggtaatg
      481 aagaaattga cctttttggc ttgattagcg ttttatgggc agcaaaaata aaaattgttg
      541 gggtgattct attttttgct tgtattggct ttcttgtttc ttttgttttg ccacaaaaat
      601 ggactagttc tgctgtagtt actcctgctg agtctgttca atggagtgaa ttaaataagg
      661 agctttccaa gcttcatgtt ctgggagtgg gttttgatat caatcgtgat agcgcatttg
      721 ctctgtttat taagaaattt cagtccgtta attcgttgaa tgagtatatg agatcatctc
      781 cttatgttat ggagattatt aaaaagaata acattagcgc tctcgaactg catcgtgcaa
      841 ttgttgagtt gagtaaaaat atgaagtcga ttgatgataa tgccagtaag aaaaacgaca
      901 agtcatcatt gtatgtatca tggactctga gttttactgc tccaacaagt aaagaagctc
      961 acgatgtgtt gtctgggtat attaattatg tttcttccct tgttgtaagg gatttgatgg
     1021 aagatataag aaataaacta gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg
     1081 atgagataaa aattagaaac cagctgaatg cagatattcg acgcctcaat tattcactgg
     1141 aggttgctaa tgcggctgga ataaaaaaac ctgtatacag caatggtcag attatgaagg
     1201 atgacccaga ttttcctgtg gctctcggtt ctgatggtat agcaactaaa ttgaacatca
     1261 aaaaatcaat caaggatgtt tcggaattga gtggggagtt gcgaaatcgt caatatgttg
     1321 tgaatcaatt ggttgtggcg aaagtggggg atgttgattt tatgccattt cagtatcaat
     1381 tgagcccaac gttaccggtc agaaaggatg ggccaagtaa tgccataatt gtaattcttt
     1441 cttctgtcat tggagggatg cttgcatgtg gctatatact tttacgtcat gccatggagg
     1501 ccagaaagat gagtgtgatt gagtaaaagt tgtgctgatg catcatttta taagcaactt
     1561 ttataaaaag ttcaaaggat cttcttgaga tcattccctt acagccgtag tcttctactc
     1621 tagaaaacga aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac
     1681 ttctgaaccg tggtaacagt cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta
     1741 gcctcagtaa ggctgccact tcaagaactc tcaatacatc tctcgcacat cctgtttacc
     1801 agtggccgct gccaatggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacgaacgc ggccataaca
     1981 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccaccg gcgcggcctt
     2161 ttttcggggc ctctcttttg ctcacatgtt ttttctggtg ttatcccctg attctgtgga
     2221 taaccgtata tccgtctttg cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgccggaag ctgcattttc tccttacgta
     2341 tctgtgcagc atttcacacc tcatatgtcg ctgttctctc accatctgct ctgccgcctc
     2401 ataattaagc cagtatacac tccgttatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg
     2521 ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa
     2581 gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc
     2641 cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc tctggctttt
     2701 gatacatcgc gccatattca gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc
     2761 gtcagttccg ggtgtcgggg ggggaccctg agcaggtggt aattgtaaga cgttgcgggc
     2821 gatggtatta caattgcaca tcctgtcctg tttctttagg acgtatcatg gcacagcata
     2881 tcaactgata aataattcct gtgcagtgca gccgctgttg cataaaattt cgctctgcgt
     2941 cgagtgtttc ttaatcaata attccgctga tactccggaa aaacgcgagt ctgttctcat
     3001 gcctgaaatg cccacacccc acgcaaaaac aagtttttgc tgatttttct ttataaatag
     3061 agagttatga caaattagtt cttcttgctc tctttgtgat atttaaaaaa gcggtgtcgg
     3121 cgcggtgttg tagctgcgcc aacaccgctt tttaggggtg gtactgacta ttttcat
//
LOCUS       DAECNO010000208         3112 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.212, whole genome shotgun sequence.
ACCESSION   DAECNO010000208 DAECNO010000000
VERSION     DAECNO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.212"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            102..3005
                     /locus_tag="KKO08_004051"
     rRNA            102..3005
                     /locus_tag="KKO08_004051"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          833 a          674 c          963 g          642 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gt
//
LOCUS       DAECNO010000209         3069 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.213, whole genome shotgun sequence.
ACCESSION   DAECNO010000209 DAECNO010000000
VERSION     DAECNO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.213"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="KKO08_004052"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="KKO08_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBD6320079.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="KKO08_004053"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="KKO08_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBD6320080.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..>3069)
                     /locus_tag="KKO08_004054"
     CDS             complement(2711..>3069)
                     /locus_tag="KKO08_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBD6320081.1"
                     /translation="NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILK
                     NELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNV
                     SSGQIMSRVKIVVMYN"
BASE COUNT          721 a          678 c          753 g          917 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg
     3001 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     3061 gataatttt
//
LOCUS       DAECNO010000210         2951 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.214, whole genome shotgun sequence.
ACCESSION   DAECNO010000210 DAECNO010000000
VERSION     DAECNO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.214"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="KKO08_004055"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="KKO08_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBD6320082.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="KKO08_004056"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="KKO08_004056"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBD6320083.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="KKO08_004057"
     CDS             2603..>2951
                     /locus_tag="KKO08_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBD6320084.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAECNO010000211         2807 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.215, whole genome shotgun sequence.
ACCESSION   DAECNO010000211 DAECNO010000000
VERSION     DAECNO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.215"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            6..81
                     /locus_tag="KKO08_004058"
     tRNA            6..81
                     /locus_tag="KKO08_004058"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KKO08_004059"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KKO08_004059"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KKO08_004060"
     CDS             complement(299..1057)
                     /locus_tag="KKO08_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBD6320085.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KKO08_004061"
     CDS             complement(1065..2168)
                     /locus_tag="KKO08_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBD6320086.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="KKO08_004062"
     CDS             complement(2178..>2807)
                     /locus_tag="KKO08_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBD6320087.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          762 a          738 c          676 g          631 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg tggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAECNO010000212         2714 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.217, whole genome shotgun sequence.
ACCESSION   DAECNO010000212 DAECNO010000000
VERSION     DAECNO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.217"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            6..224
                     /locus_tag="KKO08_004063"
     CDS             6..224
                     /locus_tag="KKO08_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBD6320088.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="KKO08_004064"
     CDS             226..591
                     /locus_tag="KKO08_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBD6320089.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="KKO08_004065"
     CDS             588..1253
                     /locus_tag="KKO08_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320090.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="KKO08_004066"
     CDS             1253..1618
                     /locus_tag="KKO08_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBD6320091.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..2228
                     /gene="hokD"
                     /locus_tag="KKO08_004067"
     CDS             2073..2228
                     /gene="hokD"
                     /locus_tag="KKO08_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD6320092.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          772 a          558 c          696 g          688 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     2161 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     2221 aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt caggcatcct
     2281 caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga atacgcaggg
     2341 tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca ttgtacttgc
     2401 acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat atttttgtct
     2461 ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa aaataaagaa
     2521 actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta tagtcgaaat
     2581 ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa aggcaatttt
     2641 tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg aattgcgagt
     2701 cagagcatcc tgtt
//
LOCUS       DAECNO010000213         2690 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.220, whole genome shotgun sequence.
ACCESSION   DAECNO010000213 DAECNO010000000
VERSION     DAECNO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.220"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            230..769
                     /locus_tag="KKO08_004068"
     CDS             230..769
                     /locus_tag="KKO08_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBD6320093.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="KKO08_004069"
     CDS             complement(995..1318)
                     /locus_tag="KKO08_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBD6320094.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="KKO08_004070"
     CDS             1604..1798
                     /locus_tag="KKO08_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBD6320095.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAECNO010000214         2646 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.221, whole genome shotgun sequence.
ACCESSION   DAECNO010000214 DAECNO010000000
VERSION     DAECNO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.221"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..570
                     /locus_tag="KKO08_004071"
     CDS             <1..570
                     /locus_tag="KKO08_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBD6320096.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMP
                     VYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKD
                     FEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            571..936
                     /locus_tag="KKO08_004072"
     CDS             571..936
                     /locus_tag="KKO08_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBD6320097.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTYAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            933..1616
                     /locus_tag="KKO08_004073"
     CDS             933..1616
                     /locus_tag="KKO08_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBD6320098.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1815..1890
                     /locus_tag="KKO08_004074"
     tRNA            1815..1890
                     /locus_tag="KKO08_004074"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="KKO08_004075"
     tRNA            1892..1968
                     /locus_tag="KKO08_004075"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
     gene            1971..2046
                     /locus_tag="KKO08_004076"
     tRNA            1971..2046
                     /locus_tag="KKO08_004076"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Thr,seq:tgt)
     gene            2050..2124
                     /locus_tag="KKO08_004077"
     tRNA            2050..2124
                     /locus_tag="KKO08_004077"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gly,seq:tcc)
     gene            2417..2632
                     /locus_tag="KKO08_004078"
     CDS             2417..2632
                     /locus_tag="KKO08_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBD6320099.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          692 a          580 c          715 g          659 t
ORIGIN      
        1 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
       61 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
      121 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
      181 ccgctgcctc acgcccacca gcaacagaat ccaccacagg aagagacggt ggtcagcatt
      241 gccgttgatc cggaatctcc ggctcagtat ctccagcgcc agaaaccaca acgggaagag
      301 atgcctgtat acacgcgctg ggtaaaaacg cagaaatgca tgacgtgtgg caatcaggca
      361 gatgatccgc accacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgacgat
      421 ttgtttgtta ttccgctgtg tcgtaaatgc cataacgaac tacacgccgg ggtaaaagat
      481 tttgaagaaa aacacggtag tcagttgttg ttgctgattc gttttttaat gcacgcgaga
      541 aattcgggtg ttctgaagtg gaaagcataa atgaccgaac gcatagaatt tgttttgcct
      601 tacccgccaa cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      661 gccggtgagc gttatcgccg tgatgtggcg ctaattgttc gccagcagcg actgaaatta
      721 aacctgtccg gaaggctggc gataaagatt attgcagagc caccggataa gcgccgtcgt
      781 gacctggaca atatcctgaa ggcaccgctg gatgcgctga cgtatgctgg actacttata
      841 gacgacgagc agtttgatga aatcaatatt gtgtgcggtc agctcgttcc tggtgggcgg
      901 ctggggataa aaatcacaga actggggtgc gcatgaataa ccagtattta cagtttgtgc
      961 gtgagcagct cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag
     1021 cctggcaaga gaatgccatg ttcgatacag ggcgttacag gcgtaaaaaa atccggtacc
     1081 gcgatgaagt gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat
     1141 cgctggcgaa ggggacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt
     1201 ggcggcgggc tgttctgtct cttgaagagc actataaagc ctggttgttg tggtgttaca
     1261 gcgggagtat ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta
     1321 atgctcaatc cggtaccaga aaaattgcag ggaaaacgca ggaacgcctg aaaaaattaa
     1381 tctggctggc ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatacc
     1441 aggagctggc attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actgggttgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatact ttgccgattt tgtacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     2041 gcaccatatg cgggcatcgt ataatggcta ttacctcagc cttccaagct gatgatgcgg
     2101 gttcgattcc cgctgcccgc tccagtcaga gtctttcagt ctgcgatgat gggaaatccc
     2161 ggagtgactg aaagacgttt aagttatgaa tgatcgcttt tttttgcaaa attgctgtgc
     2221 agaaatacta accttcgggc aggcgatcat tcataagcac tctgctttta ttccgattaa
     2281 ctgtgagtgg tttgttggat agagtgcttt ccttactgta tatatcgttt cgcccgcttt
     2341 tgcggttttt tcttttcaaa tccctttcat ttctcagtgt aaaactacgc catccgttat
     2401 ttgcggaggt gaggctatga aatccatgga caaaatttca acgggcattg cctacggcac
     2461 ctccgcaggc agtgctggct actggttttt acagtggctt gatcaggtca gtccgtcaca
     2521 gtgggctgcg attggtgtac tggggagtct ggttctgggc ttcctgactt atctgacaaa
     2581 tctgtacttc aaaatcagag aagacaagcg taaggctgcg agaggtgaat aatgtcgcca
     2641 tcatta
//
LOCUS       DAECNO010000215         2538 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.222, whole genome shotgun sequence.
ACCESSION   DAECNO010000215 DAECNO010000000
VERSION     DAECNO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.222"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..87
                     /locus_tag="KKO08_004079"
     CDS             <1..87
                     /locus_tag="KKO08_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6320100.1"
                     /translation="GRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            171..1649
                     /locus_tag="KKO08_004080"
                     /pseudo
     CDS             171..1649
                     /locus_tag="KKO08_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1646..>2538
                     /locus_tag="KKO08_004081"
     CDS             1646..>2538
                     /locus_tag="KKO08_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBD6320101.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT          660 a          601 c          629 g          648 t
ORIGIN      
        1 ggacggaagt cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat
       61 tatctgaaca taaaacacta tcagtaagtt ggagtcatta cccgcttact cgtgaggatt
      121 tatgttctgc tggcagtttc gtgatcaaca cctcgcgcca ggatggcttt atgcactatc
      181 gcaatgtggt ttgtttgttg tcttgttcat tgtttttatc gtcggcatgg ggatgtcggc
      241 tttccgcacc tgaacataat atttaccaga aacagggaaa aggcgtggtg tacctgcgcc
      301 cttataaaga aacaaatctt tctctacctg acgtgaatta caagagtttg cgtcggttac
      361 ccaatttatt aatagacccg acaacactag acgaatggga taaagaaccg cctctgactg
      421 atttgacaac cgactacctc tatgagggcg cacaggcctg gtatcctcat tattcatggc
      481 atagcgatgg tcgcaatatc ctctacgcgg gtgaagttgt gcaaaaccct cccggcacac
      541 ctcccgtcga tgtcgcctcg ttcaaggcat ggggtgattt tgccgcagat aaacacagtc
      601 tctattttga aggcaagcgc accgatgaca atggtggtgg aaatagcttg gatatcaaaa
      661 cgctgcatca ggtggaattc cggcctccct gggatcctga cttactgggg ctgatattac
      721 gtgacgctaa tttcttgtat atcaatgggc atcgcctcgc tgaccccgag agtttccgtg
      781 tgctggcgca gaagtcctgg gatcagcgag gaaagttttc caccacgttt aacccctgca
      841 ttgccgtacc atttggcccc tgggataccc tggctcgtac acggaccaaa atcctgctca
      901 acggcgaaca gcttgatgcc gacccggaca ccttttccgt cgtgcgctgg atgcccggct
      961 cactcttgac ctggcgtgat aaaaacgggc tgcagcgtaa agtcctcgac aaggaaaatc
     1021 tggcgtggga tgaagattta acaaagcact gtctggattt ttctctgctg gaaaagaaag
     1081 tgttctggcg taaaggacct gcttgtaaac aggaagaatt acccggactc gatccggagc
     1141 agtttcaacc catcagtgat gctgtcgccc agcatcagga ctcgctttat accatcatcg
     1201 aaacagagtc tggtgaccgt aagctggaga tcgtgaaact tgatgatccc aatcttatta
     1261 tcaacaaacg tttcaacgcc gggaaacgcc acggctattt acttacgcgt gccgaagggt
     1321 agccatacca ttccggttta cacgtgtttg aatctgacgg accgctgatc ttactggata
     1381 accgttctcc ggatgaacgc gaagcccatc ttaatgacca tccctttttg cgcagatggt
     1441 atgcccgcga taaccgctac atttacagct ttgatggcgc gcagctctgg cgataccgca
     1501 ccgctgatcc gaaacaagtt cgcttaatct ggaaggaaca acattcggga tatgtctatg
     1561 gcgtaaatta caaaacggga tatctggacg gaaaaattac tgatgacggc gaatttattc
     1621 ctgccccgcg caatgaggcg acaaaatgaa gggtcaaata atcacagttg cactcatcct
     1681 gttgggacac acatttctga cgcctgctgt acaggccata gggttcgact attacaatga
     1741 tcacggtgtt atgtcttacg gtaaaggtta ttggggggat aagaaaatca ttgcccagtt
     1801 tccggagatg agcagggccg gtttgcgcct tgtgaaaaat atctctggga aaacgggtta
     1861 tataccttcc gatgaaataa gcatagaaaa tgaggaatac cactgggtga cagacggccg
     1921 cgttattctc tggcgtggca aaatcgttag caatccaccg ggaacactca ctgtcgatat
     1981 tgccagcttt caggctatgg gccgcttcgc ggtcgataaa tatagcctct attttgacgg
     2041 acagcgcacc gaaagtaata gcggcgcgtc acgtgttgag ctggcgacac taaaagctat
     2101 cgaaggtaac tctaccacgc tggtggatag taaaaatctc tacttgtccg gtcgacgtca
     2161 ggggagcagc agtgatgtta ctgtattaga aaaaagatgg tggggtatta atccacgtct
     2221 tatgagtgta aacagaaatt catattccaa tgatctgctt atccgcagtg ggcagaatat
     2281 ttatttaaat ggcgttcacc ttacggcgaa tgcagactca tttgagataa ttcgctggat
     2341 acctcattca ctgctggttt ttcgcgacaa taagggtctg catcgttatc cctttggtca
     2401 attatcaggc aaagcgatac ccgtagatga tgacgtctct tttgaagtag gggaaagtcg
     2461 cgttcgctgg cgtaaacagc tcacgcccga ccgtcagtgg agcaagtgga tagacctacc
     2521 aggtattgaa cctgaaca
//
LOCUS       DAECNO010000216         2390 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.223, whole genome shotgun sequence.
ACCESSION   DAECNO010000216 DAECNO010000000
VERSION     DAECNO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.223"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..107
                     /locus_tag="KKO08_004082"
     CDS             <1..107
                     /locus_tag="KKO08_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBD6320102.1"
                     /translation="QTHLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            100..1059
                     /gene="ssuA"
                     /locus_tag="KKO08_004083"
     CDS             100..1059
                     /gene="ssuA"
                     /locus_tag="KKO08_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBD6320103.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..2201
                     /gene="ssuD"
                     /locus_tag="KKO08_004084"
     CDS             1056..2201
                     /gene="ssuD"
                     /locus_tag="KKO08_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBD6320104.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2210..>2390
                     /locus_tag="KKO08_004085"
     CDS             2210..>2390
                     /locus_tag="KKO08_004085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HBD6320105.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRIFPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          527 a          653 c          672 g          538 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     1801 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     1861 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     1921 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     1981 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2041 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2101 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2161 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2221 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2281 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtatttttc cttcaccgga
     2341 aggagtggtg acggcgttct ggacgctctc cgccagcggc gaactgtggc
//
LOCUS       DAECNO010000217         2373 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.224, whole genome shotgun sequence.
ACCESSION   DAECNO010000217 DAECNO010000000
VERSION     DAECNO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.224"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..823)
                     /locus_tag="KKO08_004086"
     CDS             complement(<1..823)
                     /locus_tag="KKO08_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD6320106.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="KKO08_004087"
     CDS             complement(1424..1672)
                     /locus_tag="KKO08_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD6320107.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="KKO08_004088"
     CDS             complement(2027..>2373)
                     /locus_tag="KKO08_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBD6320108.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          610 a          437 c          577 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggtaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttaggtaa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaaaacgc ataaaaatcc
     1681 ctctgcactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg tgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DAECNO010000218         2334 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.225, whole genome shotgun sequence.
ACCESSION   DAECNO010000218 DAECNO010000000
VERSION     DAECNO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.225"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..266)
                     /locus_tag="KKO08_004089"
     CDS             complement(<1..266)
                     /locus_tag="KKO08_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6320109.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLG"
     gene            complement(308..1196)
                     /locus_tag="KKO08_004090"
                     /pseudo
     CDS             complement(308..1196)
                     /locus_tag="KKO08_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            1673..1909
                     /locus_tag="KKO08_004091"
     CDS             1673..1909
                     /locus_tag="KKO08_004091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320110.1"
                     /translation="MSALKETASMLKLFNEAKTQAEVDRAVSEINSKLSSIQLEYLSL
                     IDELTISKSNEAALKAKLTEIEKFKFQSEDCEPR"
BASE COUNT          618 a          556 c          545 g          615 t
ORIGIN      
        1 ttgccgagca ggcgctggag ttctttaatc tgcttcatgg cggcagcaag ttcagaggca
       61 ggaacaacct gttctccggc ggccacagca gtaagacttc cttcctggta ttgcttacgc
      121 cagagaaata actggctggc tgctacacca tgttgccggg caacgaggga gaccgtcatc
      181 cccggttcaa agctctgctg aacaattgcg atcttttcct gtgtggtacg ccgtctgcgt
      241 ttctccggcc ctaagacatc aatcatctgt tctccaatga ctagtctaaa aactagtatt
      301 aagactatca cttaaataag tgatactggt tgtctggaga ttcagggggc cagtctagca
      361 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
      421 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      481 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      541 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      601 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      661 aaaacgaagc cgtttttggt gatttggtac attggacgtt tttcgccttt ggtgtcggtg
      721 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      781 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      841 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      901 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      961 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
     1021 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
     1081 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
     1141 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
     1201 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
     1261 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
     1321 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1381 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1441 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1501 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1561 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1621 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1681 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1741 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1801 tctcattgat gagctgacaa tctctaaaag caatgaggct gcgctcaaag caaaactcac
     1861 ggagatagaa aagtttaagt tccaaagtga agactgcgaa cctcgctaat tgattactgg
     1921 aacgattgtg tatgccagac agtgcaatac tagctctggc aagattacca tgtatttttg
     1981 tccagaatgt tttgcgaaga gagcaatatc tatattgcaa cctttcccta tcgaggaagg
     2041 cgataaatat acgaaaactc aatgcccctc ctgtaaaaat gagtatctca tggactacaa
     2101 tttcaataat gatttttgag tcaaggtatt cagattttta aagaacatgc cggatactca
     2161 cccgtgtccg gcgcacgcac tccacctcac ccgtggagaa ctccttaatt accaaccttg
     2221 gcttcgttgg ttagccatta acgctggtat gtaatcattc tggcaatgct taatttgaag
     2281 tggcacactg aatttggcca cctgaacaga ggtgatatgc tcacctcaga acaa
//
LOCUS       DAECNO010000219         2325 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.226, whole genome shotgun sequence.
ACCESSION   DAECNO010000219 DAECNO010000000
VERSION     DAECNO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.226"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="KKO08_004092"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="KKO08_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HBD6320111.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="KKO08_004093"
     CDS             276..452
                     /locus_tag="KKO08_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320112.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="KKO08_004094"
     CDS             527..802
                     /locus_tag="KKO08_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320113.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="KKO08_004095"
     CDS             902..>2325
                     /locus_tag="KKO08_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HBD6320114.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAECNO010000220         2258 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.227, whole genome shotgun sequence.
ACCESSION   DAECNO010000220 DAECNO010000000
VERSION     DAECNO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.227"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..470
                     /locus_tag="KKO08_004096"
                     /pseudo
     CDS             1..470
                     /locus_tag="KKO08_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(574..1041)
                     /locus_tag="KKO08_004097"
     CDS             complement(574..1041)
                     /locus_tag="KKO08_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBD6320115.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(1107..1346)
                     /locus_tag="KKO08_004098"
     CDS             complement(1107..1346)
                     /locus_tag="KKO08_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBD6320116.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1481..2086)
                     /locus_tag="KKO08_004099"
     CDS             complement(1481..2086)
                     /locus_tag="KKO08_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBD6320117.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
     gene            complement(2201..>2258)
                     /locus_tag="KKO08_004100"
     CDS             complement(2201..>2258)
                     /locus_tag="KKO08_004100"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320118.1"
                     /translation="VELHDKVIGHYLNIKHYQ"
BASE COUNT          683 a          503 c          453 g          619 t
ORIGIN      
        1 caggagacat cacgtactta cgtacagatg aaggctggct gtatctggca gtggtcattg
       61 acctgtgatc acgtgccgtt attggctggt caatgtcgcc acgcatgacg gcgcaactgc
      121 cctgcgatgc cctgcagatg gcgctgtggc ggcgtaagag gccccggaac gttatcgttc
      181 acacggaccg tggaggccag tactgttcag cagattatca ggcgcaactg aaacggcata
      241 atctgcgtgg aagtatgagc gcaaaaggtt gctgctacga taatgcctgc gtggaaagct
      301 tctttcattc gctgaaagtg gaatgtatcc atggagaaca ctttatcagc cgggaaataa
      361 tgcgggcaac ggtgtttaat tatatcgaat gtgattacaa tcggtggcgg cggcacagtt
      421 ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa ccttgcttag gcctgtgtcc
      481 atattacgtg ggtaggatca caagacctac tgctatcaca attactgaga gaaaaaaaag
      541 catccgatga ttcatttatg ctgtcctaaa aaattaacaa aacaatggga aaacaggata
      601 tgtaagcaga aaagccaatg aaattggtac agcataaggc acaccgtttt cacgaattga
      661 ttttcgaaaa aaaagaagac ctgccagaac aaccagagcg ccgcaaaagg tcattgcgac
      721 gagaaaatta gccagttcat gttcatcaat agctaacgaa agaacagtta acagcttgac
      781 atcaccgccg ccaattatcc ctgcggaaaa aagaaagaag cccacgagca aaacgctccc
      841 ggctgcaacc agattggttt tttgatgctg taaaatgagt gccaaaaaaa taagcacggt
      901 taacaccagt aaataacgat ggctaataat tctgagtaat atatcccccc atgaaataaa
      961 aataagaaag agcgcaacaa ataaaagatc gactcttagt aaccatattg ctgccattga
     1021 aagactccat gtaccataca aggttgctgc cagacatatt cctttacgtc tgacagcctc
     1081 tagagagttt ttttattttt tataagttac ggattactcg ttccggaaat cgttacctgt
     1141 tgaattgctg cagcgatagc gtcgaatgca tctttaattg cgcccaggaa gcctgagttc
     1201 tgatcgccaa agatgaaagc aagcagagtt gccatagcaa caccaatcaa tgcatattcg
     1261 attgcagtaa caccacgttc atccttcgcg aaacgcgcaa agcactgttt agttttacca
     1321 tagtacttag taataaaagt attcatgttt tcctcgtata ttattattta aattcaaacc
     1381 accagcttca tctgcgtgtc accggtatcc catatttttt ggatgtggct ataattatcc
     1441 attcccgcta tttagtcttc aaaaaaaatt acatttaata tcatttatct tgattaagaa
     1501 atagtaagac taaaaaaaca agcgtcgtca gccagcacac tgctaacacg gtaagaacgc
     1561 taataaaaac actccactgc ttcgttctat accattcaat atttttgtca gtatgtttta
     1621 ttgggagcag ctcttcagta gagaattcct ggctgttatc attctcagga atgtttgcat
     1681 cagtgtttac ttccctggcc tcgctatcgt tggtatagct actactgtct tcatcactgt
     1741 cacgctcatt ctcacagaca tgtttctcga cctttcccag atatctgacg ccttttcgcg
     1801 gaatggtttt aattagagaa ctcgacaaac ctgcgacgtc taatgccttt ctaagcatgt
     1861 aaatctgccc ataataactg ctgtcgctta ccgaaccttt ctgctcccgc caaacctcat
     1921 tgataatatg ttgcttatcg gattctccct gtagtaaaag gagtaaaaat cgatagccgt
     1981 tttcagacag attgattaca ttgcctgtac tggtggtgat acgtcgagac gctgggtaaa
     2041 acttaactcc ctgccctaaa gaaaaataaa aacggtcatc gctcataagt accgacccct
     2101 gttttcttct tcacatgact actcattaca actttgtatg aagtaagatg caaaatcata
     2161 ttgtttacat taagttaagc aattaggtaa tgactccaac ttactgatag tgttttatgt
     2221 tcagataatg cccgatgacc ttgtcatgca gctccacc
//
LOCUS       DAECNO010000221         2235 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.228, whole genome shotgun sequence.
ACCESSION   DAECNO010000221 DAECNO010000000
VERSION     DAECNO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.228"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            54..2235
                     /locus_tag="KKO08_004101"
                     /pseudo
     CDS             54..2235
                     /locus_tag="KKO08_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          598 a          497 c          562 g          578 t
ORIGIN      
        1 tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga
       61 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
      121 tggccgaggt cagtgtattc aatccggcgc ttctggaaat caaccatcaa tccggagtcg
      181 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      241 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
      301 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
      361 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
      421 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
      481 accaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
      541 ggattaatgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
      601 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
      661 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
      721 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg
      781 atacgtggac ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata
      841 ctgataacga tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg
      901 caaagtccaa cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtcagccg
      961 ttccacaagg tgcttttgag atcaccgatc tcaacaccgc aagtacaggt ggcgatttgg
     1021 acgtaaccat caaagaagaa gacggtagcg aacaacgatt cacccaacct tatgcttcat
     1081 tggcgattct taaacgtgaa ggtctgacag atgttgatgt cagcgtgggt gaattgcgcg
     1141 atgaagacgg atttacaccg gacgtccttc aggcgcaaat acttcatggt ttttcccacg
     1201 ggatcacttt atatggaggt atgcaggctg ctgaaaatta tggttctgca gctctgggtg
     1261 tcggtaaaga tcttggcgct ttgggcgcaa tttctttcga tgtgacacat gctcgtgcga
     1321 attttagcca tgatgataca gaaacgggtc agtcatatcg ctttctctat tcaaaactat
     1381 ttgacgacac agacactagc ttgcgcctgg ttggctatcg ttactccacc gagggctact
     1441 ataccactca atgagtgggc atcgcggcgc aacagccctg aagacttttg ggaaacaggt
     1501 aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt tggggagaga ttatggcaat
     1561 ttatacctga cattaagccg gcaacaatac tggcataccg atgatgtcga acgattaatg
     1621 caatttggct acagcagtag ctggaagcgt ctctcgtgga acgtctcctg gagttattcc
     1681 aatactgcca gacaggggac ggggaacaac catgccagtg ataacaccag tgagcagatc
     1741 tacatgctct ctttatctgt tcctttatcg ggctggtggg gtaatagtta cgccacctat
     1801 tctgtttcgc aaaacgataa ttccggtagc tcacatcaac tcggactcag cggtacggcg
     1861 ctggaaagaa ataacctttc atggaattta atgcagtcct ataacagtca tgatgatgag
     1921 gttggcggta atatgtccct gacctatgat ggctcttatg gcacggtgaa cggcagctat
     1981 aactacagcc aaaattccca gaggctgaat tatggtatca gagggggaat tctggcacac
     2041 agcgaagggg taacgttaag tcaggagtta ggtgaaacta ttgctcttgt taaagcacct
     2101 ggggccgccg ggttagaaat agataatatg cgcggtgctg cgacggactg gcgcggctat
     2161 acggtcaaga cacagctaaa cccttatgat gaaaatcggg tagcaatcag cgataactat
     2221 ttctcgaagt cgaat
//
LOCUS       DAECNO010000222         2221 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.229, whole genome shotgun sequence.
ACCESSION   DAECNO010000222 DAECNO010000000
VERSION     DAECNO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.229"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1037)
                     /gene="ipaH3"
                     /locus_tag="KKO08_004102"
     CDS             complement(<1..1037)
                     /gene="ipaH3"
                     /locus_tag="KKO08_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBD6320119.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHGPRIYFSMSDGQQNTL
                     HRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQ
                     VAAWLEKLSTSA"
     gene            1779..>2221
                     /locus_tag="KKO08_004103"
     CDS             1779..>2221
                     /locus_tag="KKO08_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBD6320120.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          612 a          396 c          558 g          655 t
ORIGIN      
        1 gcagaggtac tgagtttttc cagccatgca gcgacctgtt cacggaatcc ggaggtattg
       61 cgtgcagaga cggtatcgga aaggcggtca aggaacgcgg aaaaggtgtt ggcgtgctct
      121 tcatgttcaa aagcatgcca tatctgtgat acatcagatt gtttgttttc cgggaaccat
      181 gctgtcacgg catcagccag ggggcgatgg agtgtattct gttgtccgtc actcatggag
      241 aagtaaatcc gtgggccgtg gtagtccggt tgggcggttt gttgcgacag agactccctg
      301 atccgtgagg acagaggatt gtcttcgagg atgatagtgc aggtcggatc aaggctaagt
      361 atattttccg gaatgtgtgt gatgcgattc tcgcggcacc ggaaaaatat ctcggtttcc
      421 tctgagtgat gatttcttac aggtacggct ggtaggcttt ccagaagatt agtacttaca
      481 tcgagcgctt ccagtgattc aggtaactca gggagaaatg tcagctggtt atttcttact
      541 gagagcactt ccagcgatgt aggtaattca ggaagcatgg ttagctgatt gttatctgca
      601 ttaatatatt ccagcaatgc aggcaattca ggaagcatgg ttagttggtt gttatctaca
      661 tcaagatgtt tcagagatgc gggtaattca ggaagtgttg acaggcgatt gtcacaggcg
      721 tcaaggtatt ccagcgatgc tggcaattct ggtaatgata ttagggcatt ctgagtaatt
      781 tccagaacag tgatttgagg tggtaagttg tcaggtagcg aggacagatt taaacgattc
      841 agttgaagct cactgaactg attgatgaga cattctttaa gtagggagac cgcttcattc
      901 cgattttcac cggggagcgc ttgtttttcc catttatccc atgctgaaaa gtaatcagca
      961 tatgtaccgg aaatagtgtt ataaaaagaa ttttgggaca atgaaaagtt attatttatc
     1021 ggtaacatga tggacattca gttaaagccg atggacaaat ggctttgtta aaagaaataa
     1081 gtaggagttt ttatgaagtt cattccatga aatgaacttc agcacttttt tattataaaa
     1141 ggacaaacaa aattggctgt aaaaaaacga tactgtttca gttaactgtg aatagcttgc
     1201 ttgcaccgta ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacgtt
     1261 ctgccggaat gcattatcaa tagaggtaaa gtcgcaaccc caaatcgtaa aggaaaccgt
     1321 agcacgtcgt atgcaagaac gtgccacggc tggctgatgg atgttcgata gcgcgagttt
     1381 gaatgaaaat cagccggaga tgattttaca taattgctac ggaattattc aatacaggaa
     1441 ttgcttgcgt ctgcatggat tgacctgaaa tattcccgaa aatttctcta aaaaactcga
     1501 aaaaaatggt aactggttga atgtattaat atgcaatggt acgtgtcagg gattaaaaga
     1561 tgaacgtaaa tttattcaac gcattaattt taaagggttt tattgtttgt tgacgaaaac
     1621 aggaatcatg ttcggtctct ttttatctgt taaaagccag aagcatttcc ttcgctgact
     1681 ttatagtcaa ccataacaca cactctactg tctgagtcca gcgtttttta acattcttgt
     1741 taagattatg tgatctttag cgcgggagga aaatattgat gaaacagcct gcgcccgttt
     1801 atcagagaat tgcgggtcat caatggcgac atatctggct ttctggcgat atacacggtt
     1861 gtcttgagca gttgcgccgc aaattatggc attgtcgttt tgatccgtgg cgagatttac
     1921 ttatctcagt gggagacgtt atcgatcgtg ggccgcaaag tttacgttgt ctgcagttac
     1981 tggaacaaca ttgggttcgt gcggtaagag gcaatcatga acagatggcg atggatgcgc
     2041 tggcattcca gcagatgtct ttgtggttga tgaatggcgg cgactggttt attgcgctgg
     2101 cagataatca acagaaacaa gcgaaaacgg cgctggaaaa atgtcagcat ttgcccttta
     2161 ttcttgaagt acacagtcgc accggcaagc atgttattgc tcatgccgat tatccagatg
     2221 a
//
LOCUS       DAECNO010000223         2187 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.230, whole genome shotgun sequence.
ACCESSION   DAECNO010000223 DAECNO010000000
VERSION     DAECNO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.230"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..698
                     /locus_tag="KKO08_004104"
     CDS             <1..698
                     /locus_tag="KKO08_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320121.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAPPGIDMNGN
                     TTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFN
                     GTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPV
                     LDVSDISVKLQEA"
     gene            700..1305
                     /locus_tag="KKO08_004105"
     CDS             700..1305
                     /locus_tag="KKO08_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBD6320122.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1292..>2187
                     /locus_tag="KKO08_004106"
     CDS             1292..>2187
                     /locus_tag="KKO08_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6320123.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          532 a          507 c          610 g          538 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg
      241 gcagcccggc agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga
      301 atggcaacac aacgttcact gttgcagata aggagtacga tccgccgtat cctgaatacg
      361 tgatcagctg gcagacactc aaacccgtca gtctgcatgt cagtgtgacg ctgaaaaaaa
      421 gtgactacct gccttcagat attacccgac aggtacagca atccgtgctg gacgcattta
      481 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
      541 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      601 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      661 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tgcagaatgt ggctgccact
      721 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      781 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      841 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagttg ctggctgacg
      901 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      961 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
     1021 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
     1081 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
     1141 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
     1201 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
     1261 tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct
     1321 ggcggtcccc tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca
     1381 gaccagagag agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc
     1441 ggtctcagcg ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat
     1501 taccgcagca atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc
     1561 gggggccatt ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt
     1621 ctggctgaat accacggacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg
     1681 ggtaaatctt cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga
     1741 ccttcagaat aaagggacgg cacgggataa tcttcaggtt tacagtaaag agcagtcaga
     1801 tcaacgctat gttcatcggg aaggcgataa aataaccgga gagctgaaaa tccgtggtgt
     1861 taatgcgctg aggattttca acgaagcttt tggcctgatt tttcgtcgtt cggaagagtg
     1921 cctgcacctt attcccacca gtgaaggtca gggggaaaat ggcgatattg gtcccctgcg
     1981 cccgttcacc attaatttgc ggacgggtga aatatccatg tcgcataaag tgtctgttgg
     2041 cggtggttcg caggtcaatg gtgcgctggg tatcggcgtt cagaacgccc tgggggggaa
     2101 ttcaattgtt cttggtgata atgacaccgg atttaaacag aatggagacg gtattctgga
     2161 tgtttatgct aatagtcagc gtgtatt
//
LOCUS       DAECNO010000224         2096 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.231, whole genome shotgun sequence.
ACCESSION   DAECNO010000224 DAECNO010000000
VERSION     DAECNO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.231"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="KKO08_004107"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="KKO08_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBD6320124.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="KKO08_004108"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="KKO08_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBD6320125.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DAECNO010000225         2072 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.232, whole genome shotgun sequence.
ACCESSION   DAECNO010000225 DAECNO010000000
VERSION     DAECNO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.232"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..367)
                     /locus_tag="KKO08_004109"
     CDS             complement(<1..367)
                     /locus_tag="KKO08_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD6320126.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFE"
     gene            complement(406..693)
                     /locus_tag="KKO08_004110"
     CDS             complement(406..693)
                     /locus_tag="KKO08_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320127.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KKO08_004111"
     CDS             complement(943..1098)
                     /gene="hokD"
                     /locus_tag="KKO08_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD6320128.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1326..1991)
                     /locus_tag="KKO08_004112"
     CDS             complement(1326..1991)
                     /locus_tag="KKO08_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBD6320129.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          533 a          533 c          466 g          540 t
ORIGIN      
        1 cctcaaacga ctttagcaat gccgtatcac gggttctgcg tgtcgcaact gtattcagat
       61 attgctctgc cgcttcgctc agagctggcg tatgttcccg gcctactgat tcacacaggt
      121 aatcaacgaa accggacacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      181 tccagtaaac gaaacccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
      241 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc
      301 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
      361 gtgccatcgg ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag
      421 attgtagatg agtttattct tcagcaaaag cagaaaacca gccttaaaac caatcttttt
      481 caaaacccgt aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag
      541 tccattacaa aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct
      601 aatgcggcaa tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc
      661 actaaaaatt tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct
      721 ctcccctgtt cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta
      781 tctgcgaagg tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg
      841 ctgagagatc acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc
      901 agaggtgggc ggggatggga tcagctcccc acccggtcac tcttacttcc tggattcgta
      961 gtctacgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt
     1021 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
     1081 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct
     1141 acatgtgcaa agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa
     1201 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc
     1261 aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca
     1321 taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt
     1381 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
     1441 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg
     1501 cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag
     1561 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
     1621 aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg
     1681 caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct
     1741 gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca
     1801 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
     1861 tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa
     1921 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca
     1981 gcaactgtca taaatatgtt tattgaggtc ataaaaatat caaagagtgt agcattagaa
     2041 cggggctatc ggaaacagat gtgtgactgc tc
//
LOCUS       DAECNO010000226         2051 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.233, whole genome shotgun sequence.
ACCESSION   DAECNO010000226 DAECNO010000000
VERSION     DAECNO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.233"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(47..316)
                     /locus_tag="KKO08_004113"
     CDS             complement(47..316)
                     /locus_tag="KKO08_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBD6320130.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            373..1041
                     /locus_tag="KKO08_004114"
     CDS             373..1041
                     /locus_tag="KKO08_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBD6320131.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1357..>2051
                     /locus_tag="KKO08_004115"
     CDS             1357..>2051
                     /locus_tag="KKO08_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBD6320132.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          618 a          450 c          381 g          602 t
ORIGIN      
        1 cgcagcacgt cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg
       61 gttcttccgg ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc
      121 gatacttctt ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc tccagatcta
      181 cagcatcctg aagtggcgca atatgctcac tgaatccctg gatgtagaac tgtgtggtga
      241 cggtcttcca gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc
      301 tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa
      361 taaaactata atatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac
      421 acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca
      481 tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac
      541 catctggata ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg
      601 atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa
      661 tcaaaaatta aacataaaat tagccatgat gtttttgaac cttatcctgc ggcgttacat
      721 ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct
      781 acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgatgatgg aactctatac
      841 ggagccacaa ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc
      901 atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat
      961 atactctcag agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt
     1021 tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctacg
     1081 gtttccttta cgatttgggg ttgcgacttt acctctattg ataatgcatt ccggcagaac
     1141 gttcaaatat gaacgtacga tatttaacta accgaaaaac aaaatatagc acaggcgaga
     1201 catttaccat ctaagaaaat agtatcgttt ttttacagtt aaatcaacat cacttcctta
     1261 aaatgaaaac aataaatttc aatcctgaaa cattttattt cagcttatta ttccccagta
     1321 tcacatttta tcaggatatc cttctgcgag gttattatgc ttcctgtaaa taatcccccc
     1381 ctatccactg gaaacgtctc tttttacaga actacatcaa tcgacaatgt tcacaataat
     1441 tatctctccg aatgggttga atggactaaa aacagcattt ccggagaaaa cagggaaact
     1501 gcttttaccc ggctccaatt atgtctggag aacagtgaaa catcgttgga cttatcttgt
     1561 ttaggtctca gatctctacc acgattgcct gacaatcttg atgaaattaa tgtaagcaat
     1621 aaccaactat caatgctccc cgagctacca agggcattga aagagctgaa tgcaagcagt
     1681 aatcaattat ctgcacttcc tgaattacca gtgtcgctgg aatatataaa tgtgagtgat
     1741 aaccatttgt tcgcacttcc tgaattacct gcgtcactag aatatattaa tgtaagtgac
     1801 aatcacctgt ctgtacttcc gaggttacca atgtcattgg aattacttga tgcagccaga
     1861 aatgctttgg aagtaatacc agattttcca gaaagagatg atcatattat aagaatattc
     1921 tggcttaatc agaaccggat cacggcaatt ccggaaagca tacttggcct cagttctgat
     1981 agcgttgtca atcttagaga aaatcaacta tctcccagaa taatgcaaac tttgttacaa
     2041 caaaccgccc a
//
LOCUS       DAECNO010000227         2033 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.234, whole genome shotgun sequence.
ACCESSION   DAECNO010000227 DAECNO010000000
VERSION     DAECNO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.234"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..363)
                     /locus_tag="KKO08_004116"
     CDS             complement(<1..363)
                     /locus_tag="KKO08_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6320133.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="KKO08_004117"
     CDS             complement(430..1644)
                     /locus_tag="KKO08_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBD6320134.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          412 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DAECNO010000228         2031 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.235, whole genome shotgun sequence.
ACCESSION   DAECNO010000228 DAECNO010000000
VERSION     DAECNO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.235"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>2031
                     /gene="sat"
                     /locus_tag="KKO08_004118"
     CDS             <1..>2031
                     /gene="sat"
                     /locus_tag="KKO08_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HBD6320135.1"
                     /translation="GMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEK
                     ITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIV
                     NWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVI
                     LNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAI
                     NNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHA
                     TEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKT
                     GVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDF
                     KQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQ
                     NSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTG
                     NIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAI
                     WHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          745 a          358 c          401 g          527 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca g
//
LOCUS       DAECNO010000229         2002 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.236, whole genome shotgun sequence.
ACCESSION   DAECNO010000229 DAECNO010000000
VERSION     DAECNO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.236"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(66..611)
                     /locus_tag="KKO08_004119"
                     /pseudo
     CDS             complement(66..611)
                     /locus_tag="KKO08_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1266..>2002)
                     /locus_tag="KKO08_004120"
     CDS             complement(1266..>2002)
                     /locus_tag="KKO08_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320136.1"
                     /translation="NGAFNPAKDAPSTTVIKGASVTVTPVLNPETNKTGILCSAATHV
                     SDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTSDLQIIEAAPLSGKPSDQ
                     AEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAAQRAGHEGISELLGCTVCGY
                     AGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVVDVLATGSVK
                     VKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          563 a          456 c          433 g          550 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcagaattc catccagtcg ttttgggttg ctaatgatgt cctgcatccg cttaatctgg
      121 tcgggcgtag acatataacc ccacagggtg agcagaacct tttttgcggc tacactattt
      181 ccttggtctg atactaatgc catatagccc cataaactat ccctcagcca gacagcatca
      241 tagtgggttt ctgccgctgt tttgtcatct tcgaaagtag aagcaagaat aaacgttccc
      301 cagggtttct ccgtcaacgt taactttcgt gtgttttctg ttaatgttag caatccttga
      361 gtttcgtctt tggtatagtg actctttatt tcatttttta atttggctga gtgaatcaga
      421 agacgataat taggatactt atctggattt aacgataaat aacttttatc tgttgctgcc
      481 cccctggaat taagaatgtt aaatacagat tcgcttgtca cttctgcctc tgtcgcctgg
      541 catgtgccgc acgaaaatat accagcgagc agaagagtta atgcagattt ccctttcata
      601 aaataatcca tcaatcgttt gttaaaagcg tagataacca cttattcaac aacttgaaaa
      661 taaggtattt tacctgggtt gttacaaaag gattgcattg cgtaaacgct ttttatttac
      721 aaaaaaatgg ggaagtgtta cagcgattgc gaaaagcctg aagtgattga attaagataa
      781 tatagaaaat cagatacagc aaaaatggca gattgatcag gtgaagataa attggagcgg
      841 gcgaagggaa cctaggtatc gagggtatcg aagataatca gccgctaacc atttgtttcc
      901 ttaaaggatg atgaatcctt gtggttacag ttatggttac acattaattt atctctggtc
      961 aagtcccgaa gtcctaactt taagacagtg gccccaatcg tccagacttg aaaggggcct
     1021 atgggggaag tcgctgacct ttatctatta aatacctaca gagatttttg tgagattttt
     1081 taatccaagg tcataaccta ctgcccccac aggccgcaaa gtgaagacct gtaagcacca
     1141 gagcaatagc gagtatctat cactccctgt agagtatctg gtagaccaag aggacacaga
     1201 ggacgaccat atacaactcc tgtaagctca tggatgtact ccttatgttc actcagggtt
     1261 tatagttact ctgcggcagt aaaacgaata acatggacca acgggaaaga ggcgtcttcc
     1321 tcaacgtcaa actcacctgc tttcacctta acggaaccag tagccaacac atcgaccaca
     1381 ctgattaact caaggtcata cccagcagtg ttgctatttc gggtcacttt gacatctacc
     1441 gtcttagcgt ctttagttga cctgaaagag atagaagcga accgcttagg caggagatag
     1501 acacctgtat catcgcccgc atagccgcat acagtgcagc ccaatagctc actaatacct
     1561 tcatgacctg cgcgttgagc ggctcggtcc aagattgcct caagtttctc agggaccaat
     1621 acagcgtaat cgctcagtgt atttcccaac tcgaccggaa gattcaacgc taatgtatcc
     1681 agaagatctt cagcttgatc agacggttta ccacttagtg gggctgcctc aatgatttga
     1741 aggtctgacg tatctttcag gacatcgacc acagcggcga acatctggcg gcagtattga
     1801 acctgcatat cgcatacagt ctggattaat gccccctcgg tccaaccgcc catcatatcg
     1861 aggtcagaca catgagtagc agcagaacac aggatgcccg tcttattggt ctcagggttc
     1921 aacactgggg tgacagttac gcttgcccct ttgatgactg tggtagacgg agcatcctta
     1981 gctggattaa acgcgccatt at
//
LOCUS       DAECNO010000230         1979 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.237, whole genome shotgun sequence.
ACCESSION   DAECNO010000230 DAECNO010000000
VERSION     DAECNO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.237"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..87)
                     /locus_tag="KKO08_004121"
     CDS             complement(<1..87)
                     /locus_tag="KKO08_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBD6320137.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLD"
     gene            complement(778..852)
                     /locus_tag="KKO08_004122"
     tRNA            complement(778..852)
                     /locus_tag="KKO08_004122"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(817..819),aa:Gly,seq:tcc)
     gene            complement(856..931)
                     /locus_tag="KKO08_004123"
     tRNA            complement(856..931)
                     /locus_tag="KKO08_004123"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(896..898),aa:Thr,seq:tgt)
     gene            complement(934..1010)
                     /locus_tag="KKO08_004124"
     tRNA            complement(934..1010)
                     /locus_tag="KKO08_004124"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(974..976),aa:Arg,seq:tct)
     gene            complement(1012..1087)
                     /locus_tag="KKO08_004125"
     tRNA            complement(1012..1087)
                     /locus_tag="KKO08_004125"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1052..1054),aa:Ile,seq:cat)
     gene            complement(1264..1953)
                     /locus_tag="KKO08_004126"
     CDS             complement(1264..1953)
                     /locus_tag="KKO08_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBD6320138.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          526 a          522 c          414 g          517 t
ORIGIN      
        1 atccaaccac tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc
       61 tgttgatatt ttttccatct gatacatatc ccgccccccc gacagacctg tgctatcgga
      121 aagaaaaaag gccatcagca aaactctgat ggcctgaatc acctttacca atattgtatg
      181 aaaaaacacg cacgactaat tgacaataat tttcatttcc attaaaaaag atcgcgtagc
      241 attcttaatt catgaggaac tttacccgcc agcaatctga gtagcgaaag ctgttcatcc
      301 ccaatgtttt tgctggcggg tccttttttc tacggtcctc ctccccggag gggggcattt
      361 cattattttt ctaatcgtta ctggtgagaa ggcggaagaa aaagccagtt cttcgcccct
      421 aaataacagc atgattccag tccatgcaaa atctgatttc cagctcacac ataccgagca
      481 taccgagcgc tgtacaaaaa accgccagcc aaggcagtta agagtgtgtt cccggggttt
      541 gcttagaata ttttgtaagt tgtccgaaga gatatttaca acaccagaat gatgaatcat
      601 cagtcccctg ccagaggttc actacgtaac ctgaccgaca atgcaccatt ctggtgctga
      661 aaaaagagca ctggaactgc aacaaaacaa catcacagaa caggagatgg taaggagtca
      721 gacattccca cacaatattg tgtcagtgca aataacaacc ttcgtctcaa atctcgctgg
      781 agcgggcagc gggaatcgaa cccgcatcat cagcttggaa ggctgaggta atagccatta
      841 tacgatgccc gcatatggtg ccgactaccg gaatcgaact ggtgacctac tgattacaag
      901 tcagttgctc tgcctgctga gctaagtcgg cgctggccca ccaccgagga ctcgaacctc
      961 gcaccgtcaa cttagaaggt tgatgctcta tccggatgag ctagtggtgg ttggtggccc
     1021 ttgctggact tgaaccagcg acctggcgat tatgagtcgc tcgctctcac cactgagcta
     1081 aagggccgga ggcagaataa taaccatatg tcatcacatc tgcaaactca tctgaccacc
     1141 agcacgttta acgtcctgtg ccatttttca ggcataaaaa aaccgctcgg cggcgggttt
     1201 tgcttacttt accatcgcgt acaaaatcgg caaagtatca gatttgcatg agatatatgc
     1261 cttttaatct acttttgcaa tactttgctt tgaaaatgcc gctttttgtt ctgaacgtgt
     1321 tctcatcaca aacaataaag cctcactatc cagtcggtga aaaatgtgtt tcattacaac
     1381 ccagtgacca gtaaatgttt tggaccagtt tttggttgtt actcccgcca gtaatgccag
     1441 ctcctgatat tcataacctt ccccaccaaa aagttctgct tttaccgcct gcgccgccag
     1501 ccagattaat tttttcaggc gttcctgtgt tttctctgca attttcctag tgccagaatg
     1561 agccttaaac tcttcccacg cccagtgggt tatggtgatc tgatactccc agcaaatact
     1621 tccgctgtaa caccacaaca accaggcttt atagtgctct tcaagagaca gaacagcccg
     1681 ccgccacgat gatgtcgaaa acgcaacctg actgaccaga ggaattgacg cccccttcgc
     1741 cagtgattgt tttcccggga ttggtggatt atcccgcgtt atcatttttc cggtcacctc
     1801 atcgcggtac cggatttttt tacgcctgta acgccctgta tcgaacatgg cattctcctg
     1861 ccaggcttca agctgacctt ttgtcgcccc actcaaatca gcggtggcga taatgagctg
     1921 ctcacgaaca aactgtaaat actggttatt catgcacact ccagctctgt gatttttat
//
LOCUS       DAECNO010000231         1913 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.238, whole genome shotgun sequence.
ACCESSION   DAECNO010000231 DAECNO010000000
VERSION     DAECNO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.238"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(53..1702)
                     /locus_tag="KKO08_004127"
     CDS             complement(53..1702)
                     /locus_tag="KKO08_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBD6320139.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAECNO010000232         1846 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.239, whole genome shotgun sequence.
ACCESSION   DAECNO010000232 DAECNO010000000
VERSION     DAECNO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.239"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..254)
                     /locus_tag="KKO08_004128"
     CDS             complement(<1..254)
                     /locus_tag="KKO08_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320140.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="KKO08_004129"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="KKO08_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBD6320141.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="KKO08_004130"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="KKO08_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBD6320142.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="KKO08_004131"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="KKO08_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBD6320143.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1465
                     /locus_tag="KKO08_004132"
     CDS             1250..1465
                     /locus_tag="KKO08_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320144.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            1568..1846
                     /locus_tag="KKO08_004133"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="KKO08_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          506 a          408 c          466 g          466 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcactt aacccgcttc ggcgggtttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctttat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tctgaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaa
//
LOCUS       DAECNO010000233         1834 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.240, whole genome shotgun sequence.
ACCESSION   DAECNO010000233 DAECNO010000000
VERSION     DAECNO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.240"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..181)
                     /locus_tag="KKO08_004134"
     CDS             complement(<1..181)
                     /locus_tag="KKO08_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD6320145.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(322..876)
                     /locus_tag="KKO08_004135"
     CDS             complement(322..876)
                     /locus_tag="KKO08_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBD6320146.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(873..1163)
                     /locus_tag="KKO08_004136"
     CDS             complement(873..1163)
                     /locus_tag="KKO08_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBD6320147.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1163..1762)
                     /locus_tag="KKO08_004137"
     CDS             complement(1163..1762)
                     /locus_tag="KKO08_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD6320148.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          497 c          422 g          470 t
ORIGIN      
        1 ggttatgggt gatcggggag agagtttcaa cagagatttg agtggtcata acgataactc
       61 cgtacatttg gaaattatcg ccaccgacga cgccaatcga actggtggcg aactgtgcag
      121 ggttggcgta accgggtacg gaaaccggcg agcctttcgg ctcccccaca cagcccgcca
      181 taaatcgcga atgtgactgt gcaaacgata tgaaaaaaga cgcgggcgcg tctcatatcg
      241 ctccgtaaac atccgggacg ccaatcccga cgccagattt tgctggcgcg tgaggaatat
      301 agccccggat aacagattga gtcaacaaac ggtttttaga tccccggaag agaatgcatc
      361 acgcatcggt agatagagca tatattcagc aagatttagc catacatcta cccgactgca
      421 gcacgtccgg aaacaccact cagggtgtgc gtcattcagc aattcagcca ttttacgctt
      481 agtcatcccc cgcccttcat accgttgccg gaggacacta atcaatccag gatgctctgc
      541 cagcacctca cttatgactc tatcaataca taacgcctct gcatcagtac aatgcgccag
      601 ccaggtcttt tgcttgccat tgatcatctc tcgcaaaaac gcttccagct caggtttctc
      661 tattcccgct tttttcatcc tgcgcagggc ttcattgacg gctgttttcg tcaatttttt
      721 ggatgccagc aactggttaa acatatttcc cgtcttaccg ccgccaatat acgaccagcg
      781 cccccacatg cgcagttttc cctgaatcca gacactttcc agcgtggtga gacgaaggtg
      841 ttccccgctt ttgcctgtat ttgttgggta aatcataaat aaccttcctt tctccagatt
      901 tcttgtgtgc gaaaaacacc ttctgcatgc atcaggcgta attcttcttt ggtgtaatcg
      961 ctggttttta cccgcccgtc gattaaatcg tggcatgagc tacaggcaat cgctgcctgc
     1021 atatcgtgtg gttttgtcgc tgttccgcac gtccccgcca gcctgtaatg cgccagcaca
     1081 gaagtttcgg gattgtgatt gcagtagcca ggaattctga cggtgcacat ctgcccccgc
     1141 gccgctttac gtaaatccac cattacgcaa actccagtaa ctgcgcggcc acattttcga
     1201 cttcctccgg agaggaaaat ttacggaaca ggatccagtt ccacagcaca ttcagtacag
     1261 atttataaac ctgctgaaac tcggtttcgt ccatgttcgc aaatgcgata gattttgccc
     1321 tgcgcccacg actaccatca ggataaatat gctcggtgta aaatccggcc tgaatggtta
     1381 cccactcgcg gaaagcgtca aacgacttta gcaatgccgt atcccgggtt ctgcatgtcg
     1441 caactgtatt aaggtattgc tctgcggcat cactcagggc tggagtgtgt tcccgaccta
     1501 ctgattcgca caggtaatca acgaagcctg ataccagttt tcgttcgcga ggcgtgatcg
     1561 ccccaccgac cggagtccag taatcaaatc ccagttgcag gagtttgaaa aaacgcttgt
     1621 ggaatgcgta gttacgcaca cgcttaaagt ctgcgtgtat ccactcacct attttgattt
     1681 gatgcaaaaa atcgcaactc tccggcgtcg ccgggagaag taatccggaa gaggtttgtt
     1741 tgaccagttg tatatgcgcc atcgtagttc tccgctggcg cagtagaatg ggtgttcagc
     1801 ccgttatgta gtataccaga attaatgcca atac
//
LOCUS       DAECNO010000234         1805 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.241, whole genome shotgun sequence.
ACCESSION   DAECNO010000234 DAECNO010000000
VERSION     DAECNO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.241"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            173..1198
                     /locus_tag="KKO08_004138"
     CDS             173..1198
                     /locus_tag="KKO08_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBD6320149.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1805
                     /locus_tag="KKO08_004139"
                     /pseudo
     CDS             1266..1805
                     /locus_tag="KKO08_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          461 g          503 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgc
//
LOCUS       DAECNO010000235         1804 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.242, whole genome shotgun sequence.
ACCESSION   DAECNO010000235 DAECNO010000000
VERSION     DAECNO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.242"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..1135)
                     /gene="pdeF"
                     /locus_tag="KKO08_004140"
     CDS             complement(<1..1135)
                     /gene="pdeF"
                     /locus_tag="KKO08_004140"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBD6320150.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
     gene            complement(1317..1469)
                     /locus_tag="KKO08_004141"
     CDS             complement(1317..1469)
                     /locus_tag="KKO08_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320151.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1487..1678
                     /locus_tag="KKO08_004142"
     CDS             1487..1678
                     /locus_tag="KKO08_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBD6320152.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          567 a          408 c          369 g          460 t
ORIGIN      
        1 attgaatccg cagcatgatg ccatagttct taaccagcat ttccatgcca gggatgcgta
       61 aataacaaag cgcagaccag ggggcatcac gcaacgcgcg attcagggcg cgaacatttg
      121 gcagatgaac caccgggtcc acatacgcaa gccgctgaat gcgtctgact accgctcgct
      181 gacgagttgc cagtacagcc atataattga caataaaaga gaataccaga taactggagg
      241 aggttatggt cagctgcgtg gtatagccag gataaatggg aatgtaattt tgatagctgt
      301 ggatgctgat catcaacacg accgcccaga gcagcgaaat cagcttataa ccatagcgca
      361 ttgctcccca catcatcagg ggcagcaata atgacaaggt ataattggtg ctaaaaattg
      421 tgcttttttc atttaacggc atgcataaca gcaacaataa agcacctaat gccagtagcc
      481 agagcgcgaa ctcttttttg gtgactttgg catcaacctg ctgttttaat tgcgaataat
      541 agctacgtaa ataaaatgga tttcgcacta cccgaatgat gaagtagcac agcgggacac
      601 cgatcagatt acccaccagt aaggcctgat aattgattaa agtcccgagg ttaaaaggca
      661 tgacaccgac cagattttct ctgctcgcca gtaatcctac aaatgcagca aactggaaaa
      721 gtatcagaaa cagcgttgca ggaaacacta tctgccagaa tatacgctgg gaaattaaac
      781 gggtatcacc atgtgaaacg ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac
      841 agagaatgat aatgataata aaattagccg ttaatgagag cgtttcgtaa aaacccagct
      901 ctgcatattt gcggacaaaa atccctaagg tgatccctgg caatgccgcc cagctgaaaa
      961 acatcatcat gctgatcatc agtgccaaag gcagataaaa gagaaaaacc tcaccggaag
     1021 aaatatgcgc gaaagtatta atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca
     1081 gcggaagccc ccaccattta tcacgtattt ttatataagt tgcattcagt ttcatagatg
     1141 ctcagcagaa tcccccacat cctgaaggag gtgtattcag acaggcatcc cacctgactt
     1201 cgaatgatga ttattcatca ctatagagag cattgattct aagtgtcata tgaaagttcc
     1261 aattgatata tatcaaacaa aataaccctg attaatgaat tattacgttt atcatgttaa
     1321 ttcatcatta ttacatcatc attgtaaata attaaattaa cttccataac attaaaatat
     1381 gtatccactg acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta
     1441 atgtaagtac cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac
     1501 tagtatgcga aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag
     1561 ctttatcttc ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc agcaccatca
     1621 gagcaaaaaa gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg
     1681 gttaccttct ccactttcac agaacataac ggcacttcgc tgtcggatgc ttttgctctt
     1741 tgggattatc aaagcggcag atattctttc atcttaaatt ttacgtcttt gtcctgactg
     1801 atgt
//
LOCUS       DAECNO010000236         1753 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.243, whole genome shotgun sequence.
ACCESSION   DAECNO010000236 DAECNO010000000
VERSION     DAECNO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.243"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            43..222
                     /locus_tag="KKO08_004143"
     CDS             43..222
                     /locus_tag="KKO08_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320153.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            274..468
                     /locus_tag="KKO08_004144"
     CDS             274..468
                     /locus_tag="KKO08_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320154.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1267..1603)
                     /locus_tag="KKO08_004145"
                     /pseudo
     CDS             complement(1267..1603)
                     /locus_tag="KKO08_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          538 a          387 c          363 g          465 t
ORIGIN      
        1 tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt atcaccagaa
       61 gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat tatcgaacag
      121 caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc tcctttagtt
      181 gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt ttcattagca
      241 cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg
      301 gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg tgccgcgctg
      361 ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa tggcaaaaaa
      421 atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca acaatctgca
      481 tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc tttatattca
      541 ctgacgagct tgccggggca cctttgtttg ccggtcattc tggtatcatg cagatattta
      601 cggcgtacaa gcccactagc atgacattgt aataaggcct gagcctaacg ctttaatgtg
      661 tgtcatacac tcagtggcgt aatcgttgac aatatggtgc atgtaatcgt aagcgtccag
      721 cgaaatccat ctggtcataa tctgaagcat ccgacaaagt ggtgtccacc aaataagtga
      781 tctgcgccca atatcctgag ccggaacttt gacaaaggcc aacgaaaaat gggagcggat
      841 gttacagagt tctcggtaca ggggaaaagt ggtacctgtc gccagttctc cgatctgttt
      901 aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg tgatggagca tcgttaatac
      961 gatgctatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct gcatatggat
     1021 cagggttggc aatatcgaat cgcaggctat aggcaaagtt aaagccccat ggagtagcac
     1081 aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac ttcttcagca
     1141 cactgctaaa acgcatagtg atcgagcgct gattctggcg aacaactgaa ctaatacatc
     1201 agaatatgca ttatgttaaa taaatatgaa aagatggttt aaataccccg ttacttttga
     1261 cttacactat acggtatcgc atcgtttaat attcgcaccg gccagatttt tatttctatt
     1321 agttgtcaca atactgaatg cgtacgacca cagtattctg gctcctgtgt ggttatgctt
     1381 taattctgcg ttccgggcag ataagcagtt gcttgcagga atccttcttg tgttaatgtc
     1441 agttcccctt tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc
     1501 cctggattat agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg
     1561 cagatccagt ccgtttttca taacccactg tactgataac cataatctaa tcagtagaaa
     1621 ttgagtcgaa aataagcact actccataca ggataattag aggtcagttt gattattcac
     1681 aattcatcat cagcattttc tatttctgac gaaatcaaca tgaaaataac catatatgat
     1741 aattattata ata
//
LOCUS       DAECNO010000237         1745 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.244, whole genome shotgun sequence.
ACCESSION   DAECNO010000237 DAECNO010000000
VERSION     DAECNO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.244"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            452..1300
                     /locus_tag="KKO08_004146"
     CDS             452..1300
                     /locus_tag="KKO08_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD6320155.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1317..1598
                     /locus_tag="KKO08_004147"
     CDS             1317..1598
                     /locus_tag="KKO08_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320156.1"
                     /translation="MNPMLDYCFTSAPQQTITHTGIKNLLWIIKKNLQQKGDPDLIPC
                     AYGLCDRQVSPDKAYTAELIYMHGTMIRKKKRKYCSEICAEKDQMAHEL"
BASE COUNT          498 a          399 c          409 g          439 t
ORIGIN      
        1 cattatatag ttctactctg gcgagatacg ttggcatatt catttcctta ctggttgtgt
       61 gagaacttca gtaaagatac caccaaagcc cggaagtggt gaaataaaac cgggcacaac
      121 acgaaggcgc atttccggta tccataaaga gtcggtcttg tctgtgaaat ttaaatggtg
      181 ggagtgcgct tccggttgta aataacgaca ttgctgtgtg aagtaccagt tggcggcatc
      241 ggtttaattg ctggctgatg tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
      301 ggctatgcgc acgcggcaca gttaaaagtc atgttagtcc ttattggttt gggtgggaaa
      361 gccaactgta attgttaact ggttaacgtc acctggaggc accaggcacc gcatcaacaa
      421 agttcacttc ggtgatgaaa ggaaagagaa aatgttgaat gtagctattg aaaaccagaa
      481 cgggtggaat tatagtgcac ctgcacctca taaaacgggt gccgggattg ctaccccgac
      541 gatgactact gcgcataacc gcgctcaggc ggttttttta tgcgtcaagc acagccacat
      601 tcagattatg gtggggcgtg cagggcagcc gcaaggctgg ccggtttcgg tagtcaccgg
      661 ttgtagcaac cctgtgcgtc tcaccaccca tgagattgct acctctggtg gtgagtcatt
      721 taaactgact atcgaggctg ccatcatggc tactatcctt accctttctc accctgacgc
      781 aaccatcgaa aatggtcgcg ctgtcactac gtctgttgcg gttgcggagt tcttccggaa
      841 aatgcacaaa aacgttattc agaagatcga aactctcgaa tgctcgccgg agtttaaccg
      901 gcttaatttt aagcccgtca cctataccga cgccaaaggc gaaaaacgcc caatgtacca
      961 aatcaccaaa aacggcttcg ttttcctggt gatgggcttc actggcaaaa aagccgccgc
     1021 attcaaggaa gcctacattg ctgagttcga ccgcatggag gccgaactgc gccagaataa
     1081 caccccacct gctgacaaga tgattccggg tgatggtcgc actctggttg ttcacttcga
     1141 caaattcggc aatgtcgaat tcaccgaaac cgttcctgat ggcgcactgg tctgtaccct
     1201 ggagactttc cggttttatc tggaaaaaca gggatggact cttgttaacc ggggcgcaat
     1261 taaaaatatg actgtggaac aattactttc tttaaaataa aaatggagct acggatatga
     1321 accctatgct tgattattgt tttacctctg cacctcagca gaccataaca cacaccggaa
     1381 tcaaaaattt gttatggata atcaaaaaaa acctccagca gaaaggtgat cccgatttaa
     1441 ttccctgtgc ttatggcctg tgtgacagac aagtaagccc tgataaggct tacactgctg
     1501 aacttattta catgcacgga accatgataa ggaaaaagaa acgcaaatat tgtagtgaaa
     1561 tctgcgccga aaaagaccag atggcacacg aactttaatt aactgactat tcgaaactga
     1621 atttatgcca gcaatggcag ggattcgctc aaccttaatt aaggagaaaa acatgaacac
     1681 caattatgaa gccactgttg caactactga taacattgtt cacgaggttt acctggaagg
     1741 aaagc
//
LOCUS       DAECNO010000238         1728 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.246, whole genome shotgun sequence.
ACCESSION   DAECNO010000238 DAECNO010000000
VERSION     DAECNO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.246"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..243
                     /locus_tag="KKO08_004148"
     CDS             <1..243
                     /locus_tag="KKO08_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBD6320157.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(332..844)
                     /locus_tag="KKO08_004149"
     CDS             complement(332..844)
                     /locus_tag="KKO08_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBD6320158.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1039..>1728
                     /locus_tag="KKO08_004150"
     CDS             1039..>1728
                     /locus_tag="KKO08_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320159.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGID"
BASE COUNT          507 a          386 c          438 g          397 t
ORIGIN      
        1 gctgaaaatg atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga
       61 aaatgccctg tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc
      121 accgtcgaac tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc
      181 agcgaccaga cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg
      241 tagcggtaat tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa
      301 gctggattcg caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa
      361 aactaccaac ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt
      421 tcactggatg aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga
      481 taaagcgcga agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt
      541 ctattgtatt ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta
      601 taccaagatg gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag
      661 cttcccatgg tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg
      721 ctggtggcaa tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa
      781 gcccccatag gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca
      841 tcaatgtctt tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta
      901 ggcttaaaca ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc
      961 aggagtgagt gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa
     1021 atcacaggaa gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg
     1081 gccggagtcc ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc
     1141 aggaagaggg ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat
     1201 ggtacgcaaa aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa
     1261 aaaagtacgc aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt
     1321 accgatcccc cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt
     1381 tatgcccgtc gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc
     1441 gaggacgaat tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg
     1501 tggttggtgt cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat
     1561 atcagcgccg ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc
     1621 acgcttgcga cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat
     1681 aaggtgtctc tggaggctga tcgtcttcgc cgtgatgcag gtattgat
//
LOCUS       DAECNO010000239         1696 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.247, whole genome shotgun sequence.
ACCESSION   DAECNO010000239 DAECNO010000000
VERSION     DAECNO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.247"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..77)
                     /locus_tag="KKO08_004151"
     CDS             complement(<1..77)
                     /locus_tag="KKO08_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBD6320160.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFL"
     gene            complement(758..832)
                     /locus_tag="KKO08_004152"
     tRNA            complement(758..832)
                     /locus_tag="KKO08_004152"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(797..799),aa:Gly,seq:tcc)
     gene            complement(836..910)
                     /locus_tag="KKO08_004153"
     tRNA            complement(836..910)
                     /locus_tag="KKO08_004153"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Thr,seq:tgt)
     gene            complement(913..989)
                     /locus_tag="KKO08_004154"
     tRNA            complement(913..989)
                     /locus_tag="KKO08_004154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(953..955),aa:Arg,seq:tct)
     gene            complement(991..1067)
                     /locus_tag="KKO08_004155"
     tRNA            complement(991..1067)
                     /locus_tag="KKO08_004155"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Ile,seq:cat)
     gene            complement(1257..>1696)
                     /locus_tag="KKO08_004156"
     CDS             complement(1257..>1696)
                     /locus_tag="KKO08_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBD6320161.1"
                     /translation="GKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRD
                     MINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELLN
                     DAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          464 a          446 c          348 g          438 t
ORIGIN      
        1 tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc tgttgatatt
       61 ttttccatct gatacatatc ccgccccccc gacagacctg tgctatcgga aagaaaaaag
      121 gccatcagca aaactctgat ggcctgaatc acctttacca atattgtatg aaaaaacacg
      181 cacgactaat tgacaataat tttcatttcc attaaaaaag atcgcgtagc attcttaatt
      241 catgaggaac tttacccgcc agcaatctga gtagcgaaag ctgttcatcc ccaatgtttt
      301 tgctggcggg tccttttttc tacggtcctc ctccccggag ggggcatttc attatttttc
      361 taatcgttac tggtgagaag gcggaagaaa aagccagttc ttcgccccta aataacagca
      421 tgattccagt ccatgcaaaa tctgatttcc agctcacaca taccgagcac tgtacaaaaa
      481 accgccaacc aaggcagtta agagtgtgtt cccggggttt gcttagaata ttttgtaagt
      541 tgtccgaaga gatatttaca acaccagaat gatgcattat cagtcccctg ccagaggttc
      601 actacgtaac ctgaccgaca atgcaccatt ctggtgctga aaaaagagca ctggaactgc
      661 aacaaaacaa catcacagaa caggagatgg taaggagtca gacattccca cacaatattg
      721 tgtcagtgca aataacaacc ttcgtctcaa atctcgctgg agcgggcagc gggaatcgaa
      781 cccgcatcat cagcttggaa ggctgaggta atagccatta tacgatgccc gcatatggtg
      841 cgactaccgg aatcgaactg gtgacctact gattacaagt cagttgccct gcctgctgag
      901 ctaagtcggc gctggcccac caccgaggac tcgaacctcg caccgtcaac ttagaaggtt
      961 gatgctctat ccggatgagc tagtggtggt tggtggccct tgctggactt gaaccagcga
     1021 cctggcgatt atgagtcgct cgctctgacc aactgagcta aagggccgga ggcagaataa
     1081 taaccatatg tcatcacatc tgcaaactca tctgatcacc agcacgttta acgtcctgtg
     1141 ccgtttttca ggtataaaaa aaaccgcata aagcgggctc tttcaaatgt ccatgtctgc
     1201 tattcgcctc gcggtacagc tttgcgaagc gtacaagaat tgaagcagtt tttacgtcaa
     1261 aaagcaataa cttttttctc tataccaaaa gccataacca ttggtttgta caaaataaat
     1321 tctgccacct ttagccaatg ctcaatgcgt ctttcacagg ttcttaaact ccatttcggg
     1381 tgtgcatcat tcagcagttc agccattttg catttggtca tcccccgccc ctcataccgt
     1441 tgccgaagga cgctaatcaa tcctggatgc tctgccagca cctcacttat gacccgatca
     1501 atacataacg cctctgcatc agtacaatgc gccagccagc tcttttgctt accgttaatc
     1561 atatcccgca aaaaagcctc aagttcagac ttgttcagac ctgctttttt catcctccgg
     1621 agcgcctcgt taattgccgt ttttgtcagc tttttagagg tcaacatcag gttaaacata
     1681 ttccccgtct taccgc
//
LOCUS       DAECNO010000240         1650 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.248, whole genome shotgun sequence.
ACCESSION   DAECNO010000240 DAECNO010000000
VERSION     DAECNO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.248"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(181..654)
                     /locus_tag="KKO08_004157"
     CDS             complement(181..654)
                     /locus_tag="KKO08_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBD6320162.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            810..>1650
                     /gene="intI1"
                     /locus_tag="KKO08_004158"
     CDS             810..>1650
                     /gene="intI1"
                     /locus_tag="KKO08_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBD6320163.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLR"
BASE COUNT          358 a          453 c          435 g          404 t
ORIGIN      
        1 ctgcgaatat gtgagttttg acctaatgag ccacaaaaac tcattttcgc ccacgataat
       61 ttttctactt cctttctttg gaatgctcat ttcaatattc acataacgtc gctatcaatc
      121 gcggaaaagg cgtagtgcgc gaagcgaacg aagctttttg ccgtcgattg catagctttg
      181 ttaacccttt ttccaaattt gatagcaata gttaatgttt gaagtaaagt gttgctcaaa
      241 aacaacttcg aaggtatttg gaatactcgg gaagaaaaca tccccctctg gctcgatgtc
      301 gatcgtcgat aagtggagcg tagaggccat gggtaatgtt tctcggtaaa tttctccgcc
      361 accagacact ataacgtgac cggtgaattc agctagcctg tccatggcct cttcgattga
      421 ctgaaataca actacattgt catcatttga tgtccaacct gagcgggtaa cgaccgcgta
      481 tttcctattg gggagtgcgc ccatagattc aaacgtcttg cgacccacca gaagccactg
      541 attgtaggtc aatgctttaa aaagtagctg ctcccctttc gcggaccagg gtatgtctgg
      601 accgcaacca atcacgccgt ttttcgcttt cgcagccatc aatgatactt tcaaggttct
      661 catcctgggt taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc
      721 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
      781 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
      841 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
      901 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc
      961 gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg
     1021 agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg
     1081 gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc
     1141 ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag
     1201 gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt
     1261 tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca
     1321 agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc
     1381 agctgtcgcg tgcacgggca tggtggctga aggaccaggc cgagggccgc agcggcgttg
     1441 cgcttcccga cgcccttgag cggaagtatc cgcgcgccgg gcattcctgg ccgtggttct
     1501 gggtttttgc gcagcacacg cattcgaccg atccacggag cggtgtcgtg cgtcgccatc
     1561 acatgtatga ccagaccttt cagcgcgcct tcaaacgtgc cgtagaacaa gcaggcatca
     1621 cgaagcccgc cacaccgcac accctccgcc
//
LOCUS       DAECNO010000241         1616 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.249, whole genome shotgun sequence.
ACCESSION   DAECNO010000241 DAECNO010000000
VERSION     DAECNO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.249"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(349..495)
                     /locus_tag="KKO08_004159"
                     /pseudo
     CDS             complement(349..495)
                     /locus_tag="KKO08_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            549..731
                     /locus_tag="KKO08_004160"
     CDS             549..731
                     /locus_tag="KKO08_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBD6320164.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(832..1161)
                     /locus_tag="KKO08_004161"
     CDS             complement(832..1161)
                     /locus_tag="KKO08_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBD6320165.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1333..>1616)
                     /locus_tag="KKO08_004162"
     CDS             complement(1333..>1616)
                     /locus_tag="KKO08_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320166.1"
                     /translation="FVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          439 a          372 c          381 g          424 t
ORIGIN      
        1 ccggggcggt tcaaatagca atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa
       61 tgagaataac cagttccctg aaggcgacgt agcccggtac aagagtaacc cacgttagcg
      121 cttggcgacg gcggaacaaa gctgattcgc gacgtaagaa agtggcaggc tcatatgacg
      181 gacctctgta atgcggtatc caacccgcga atatcagcat gattcaccgt cggatttact
      241 gctacgtcgc cctcagggaa ttaaacctaa aaagaaaatc gtcaaaatta gagattccgg
      301 tttgacacgg aaagtcatat cagcgtcaac ggagcaccgt attgacgctt atttattggt
      361 gagaactacg ttccatggca ggagttcgtc aacacggttg gagggccatt caggcagtat
      421 gctcagaata tggcgcagat acgcttccgg atcgataccg ttcagacggc aggtgcctgc
      481 ttttccggca acaagcagga atctcagcgc tgaagcagga attagaggta aaaacgccct
      541 accgggccat gaatcacccg gtcattggag tagtaaccaa agcagattta gccagcatgg
      601 aacaaatatc tttggtgaaa agttggttac gggaagctgg agcgcacaac gtgttagtaa
      661 ccagtgcagt taataacaat ggagttacag aactttttgc cttgctgcat acagaagaag
      721 gctgttgtta atttcgattg gttctcagat gagaatgatg acggtgatac acagataaga
      781 atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacggga attacttcgc
      841 ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc ggcgttcttt
      901 ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt cacctttcat
      961 gctggcgatg atgccctgga ttgcttcaac gctcatccct ggcttgatgt aatcgctcag
     1021 gttgtccagc aacaggacgc gcttctggtt gtcacggatc tttttctcaa cgtcctgaat
     1081 ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta cgtcctggaa
     1141 tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg attcgtttaa
     1201 gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac aatgctttta
     1261 tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca agatgattct
     1321 gaagttcaga aattatttgc gcaaggcccg gcaaatcaga ttcgatagca actcaagctg
     1381 atgtgccgtt tccatgttca gccacacata accatagatt ggtgtttcca cgactttcag
     1441 gtcatggtgg ttatcgagca actgacgcag ctcttccaca gcatcgttca atttttccgt
     1501 attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaggc tcagcagtat
     1561 ttgctgcatc atgtgctggg tatcacaaag tttttgcgcg ttcaataaca cgaaat
//
LOCUS       DAECNO010000242         1598 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.250, whole genome shotgun sequence.
ACCESSION   DAECNO010000242 DAECNO010000000
VERSION     DAECNO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.250"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            154..477
                     /locus_tag="KKO08_004163"
     CDS             154..477
                     /locus_tag="KKO08_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBD6320167.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="KKO08_004164"
     CDS             474..890
                     /locus_tag="KKO08_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBD6320168.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="KKO08_004165"
     CDS             856..1380
                     /locus_tag="KKO08_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320169.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1598
                     /locus_tag="KKO08_004166"
     CDS             1377..>1598
                     /locus_tag="KKO08_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320170.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          389 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagc
//
LOCUS       DAECNO010000243         1597 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.251, whole genome shotgun sequence.
ACCESSION   DAECNO010000243 DAECNO010000000
VERSION     DAECNO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.251"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..636)
                     /locus_tag="KKO08_004167"
     CDS             complement(<1..636)
                     /locus_tag="KKO08_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBD6320171.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="KKO08_004168"
     CDS             complement(750..1322)
                     /locus_tag="KKO08_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBD6320172.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          336 c          318 g          468 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAECNO010000244         1554 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.252, whole genome shotgun sequence.
ACCESSION   DAECNO010000244 DAECNO010000000
VERSION     DAECNO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.252"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            72..329
                     /locus_tag="KKO08_004169"
                     /pseudo
     CDS             72..329
                     /locus_tag="KKO08_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(342..1493)
                     /locus_tag="KKO08_004170"
     CDS             complement(342..1493)
                     /locus_tag="KKO08_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HBD6320173.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT          392 a          362 c          345 g          455 t
ORIGIN      
        1 gaagtggcac actgaatttg gccacctgaa cagaggtgat atgctcacct cagtacaaca
       61 caggtgctcc aatgaaaaaa agaaatttca gcgcagagtt taaacgcgaa tccgctcaac
      121 tggttgttga ccagaactac acggtggcag atgccgccaa agctatggat gttggccttt
      181 ccacaatgac aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa acaccaaaat
      241 cctctccgat aacaccagaa caaatcgaaa tacgtgagct gaggaaaaag ctacaacgca
      301 ttgaaatgga gaatgaaata ttaaaaaagg ctactgtaga ttcaatctgt caatgcaaca
      361 cccctttcaa ttatctcttt cggtgttttg aacttcagtg tctttctcgg tctgttgttt
      421 agctgagcag caaccagatc tagttcatgt tgagtatatt gggcaagaca tgtcttttta
      481 ggaaagtact gccgaattag cccatttgtg ttctcatttg ttccccgctg ccaaggactc
      541 tgaggatcgc agaagtaaac tttaacgccg gtgctgacag taaattctag atgtctggcc
      601 agttccattc ctctgtccca tgtcagtgat tttctgagtt ctgacggtaa actcaggaat
      661 ttgtcggtaa gagcctgatt tactgagaca gaatctttgc ccctgagtct gaggatgatc
      721 gtataacgtg attttcggtc tacaagtgtg gctatatgag agttttttgt acctgagact
      781 aaatcgccct cccaatgccc cagagagcgt ctgttatcga tatttcggga acgttcgtga
      841 attggtgttc cgttcactat gttaatcgta cctctttcgc ctttgcgggt atgacgcctg
      901 ccatggcgaa ggctatgcga ccgtcgcaga tgctgtatat tcaggtggtg tagcgcttca
      961 cggctacgaa agtacagcgt tttataaatt gtctcaggtg atattcgcag cgttttttga
     1021 cgtggttttg ttcgccttaa ccatcctgat atttgctctg gagaccattt catctccagc
     1081 ttttccagaa caagctttcg caatggtaaa ttttgatcca gtaagcacgg ttttggcctt
     1141 ttcgccattc tgttggctcg gttattagca tcaacagctt tgtaatagcg tctgccccga
     1201 ttacgctgaa cttcacgtga gatcgtcgaa ggactgcgat tcagcgcagt agctatcgca
     1261 cgaatgctca ttttggctga caaaccagct cgtatctcct cgcgctcaga cagtgtcagg
     1321 tgagctacag cccgcttacg ctcatggggt tttatgccgc cagtatccct taacatagtg
     1381 aagatcgttc cgggttttga acccaggata ttcgctattt cactgaagcc tgttccgttc
     1441 ttccatagtt caaaaacaga ggctttttcc tctgctgtaa atgttcgtct cattcaaaaa
     1501 acctccgcaa ccccatgttt tcacataact gttgcgttga ccaattgaat ctac
//
LOCUS       DAECNO010000245         1538 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.253, whole genome shotgun sequence.
ACCESSION   DAECNO010000245 DAECNO010000000
VERSION     DAECNO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.253"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(910..1386)
                     /locus_tag="KKO08_004171"
     CDS             complement(910..1386)
                     /locus_tag="KKO08_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6320174.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          348 a          410 c          385 g          395 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DAECNO010000246         1487 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.254, whole genome shotgun sequence.
ACCESSION   DAECNO010000246 DAECNO010000000
VERSION     DAECNO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.254"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..283)
                     /locus_tag="KKO08_004172"
     CDS             complement(<1..283)
                     /locus_tag="KKO08_004172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBD6320175.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
     gene            complement(510..1052)
                     /locus_tag="KKO08_004173"
     CDS             complement(510..1052)
                     /locus_tag="KKO08_004173"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320176.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            1129..1487
                     /locus_tag="KKO08_004174"
                     /pseudo
     CDS             1129..1487
                     /locus_tag="KKO08_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    1410..>1487
                     /locus_tag="KKO08_004174"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          398 a          364 c          323 g          402 t
ORIGIN      
        1 caccagttaa cgtacaacca cttacaaaga cgaagatcgc cgactgttct atcgctatac
       61 tttgattgtt tgcagccgtg tttttgtttg cagtgttgat atgaacacca ctgacaattt
      121 tatgtgactg cgttgaggcc ggcgttctaa aatatatccc cgtaaattgt tcagtgccat
      181 cggcatctgc tgctatccat gagtttgaga aagaaaatcc tccgtttaca ttctctataa
      241 ccacgcctgt tgagccgcaa taatctaaat ccagttgttc catatgcaaa tcaaggcaat
      301 catgaacata gacggcattt ttaaaaccaa tgcacatggt ctcactgtct aaaattattg
      361 cttctgacct aactgcctct gacgaccacg catacgtttg aggctgaatc ctgatgccaa
      421 agctttcatc agcagaataa tttcctctgc tgaaatggca ggagctgaca ctaacactta
      481 cgcactggcc taaaaagtga agaccgactc tacaggttcc agcctgacat gctataaatt
      541 ttgagtgcca gagcccttgc attaaataat gcggtttcaa cttcattcac cattacatta
      601 tcaaataccc agccataaac ctgtctgatg acagagtctt ctgctgtaat gtttgtgtca
      661 ataccattgc aggtgttatt accagttact cgtatatttc tcagataacg gccgatagtg
      721 tctgaatcat aagacagcct taacacagat cctgtaagtc cgctttttgc tcggagttgc
      781 gatgccttac caactcctct gatgttaaca ccgccaggaa taagcaaagt ggatccaaca
      841 atatacccca ccgcttggtt gctcgcaggg ataaacagct ccccgccgga gggtagtgaa
      901 gcaacggcat ctatagcatt ttgtattgcc tggtaattat ctgtcacacc atcatcaata
      961 acaccaaaat ccaggatact tattgcttct ttatttttgt catgctgagt tcttaaaact
     1021 gcttttggta aaggctgctt gacgccaatc atggcatcgc ctttggtgtt atcctgaacc
     1081 gccccgggaa tcctggagac taaacttcct gagaaagagg taaacaggat gactaaaaat
     1141 actcgttttt cccccgaagt ccgtcaacgg gcagtccgta tggttctgga aagtcagggc
     1201 gaatatgact cacaatgggc gacaatttgt tccattgccc caaagattgg ctgtacgccg
     1261 gagactctgc gtgtctgggt tcgccagcat gagcgggata ccgggggcgg tgatggaggg
     1321 ctcaccaccg ctgaacgtca gcgtctgaaa gagccggagc gtgaaaatcg tgaactgcgc
     1381 cgcagtaacg atatccttcg ccaggcttcc gcttattttg cgaaggcgga gttcgaccgc
     1441 ctctggaaaa aatgatgcca ctgctggata agctgcgtga gcagtac
//
LOCUS       DAECNO010000247         1450 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.255, whole genome shotgun sequence.
ACCESSION   DAECNO010000247 DAECNO010000000
VERSION     DAECNO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.255"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..656
                     /gene="ipaH9.8"
                     /locus_tag="KKO08_004175"
     CDS             <1..656
                     /gene="ipaH9.8"
                     /locus_tag="KKO08_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBD6320177.1"
                     /translation="VAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSL
                     GREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGV
                     TANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQKYEMLENEYP
                     QRVADRLKASGLSDDADAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSQLHHS"
     gene            complement(657..836)
                     /locus_tag="KKO08_004176"
     CDS             complement(657..836)
                     /locus_tag="KKO08_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320178.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPPGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(836..>1450)
                     /locus_tag="KKO08_004177"
     CDS             complement(836..>1450)
                     /locus_tag="KKO08_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD6320179.1"
                     /translation="SLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDRD
                     QAVPYTATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRTE
                     QEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIVRDVMTWGDADNQQVKLSMPEL
                     EELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
BASE COUNT          327 a          416 c          377 g          330 t
ORIGIN      
        1 ctgttgctgc tgatgccact gagagctgtg aggaccgtgt cgcgctcaca tggaacaatc
       61 tccggaaaac cctcctggtc catcaggcat ctgaaggcct tttcgataat gataccggcg
      121 ctctgctctc cctgggcagg gaaatgttcc gcctcgaaat tctggaggac attgcccggg
      181 ataaagtcag aactctccat tttgtggatg agatagaagt ctacctggcc ttccagacca
      241 tgctcgcaga gaaacttcag ctctccactg ccgtgaagga aatgcgtttc tatggcgtgt
      301 cgggagtgac agcaaatgac ctccgcactg ccgaagccat ggtcagaagc cgtgaagaga
      361 atgaatttac ggactggttc tccctctggg gaccatggca tgctgtactg aagcgtacgg
      421 aagctgaccg ctgggcgcag gcagaagagc agaagtatga gatgctggag aatgagtacc
      481 ctcagagggt ggctgaccgg ctgaaagcat ccggtctgag cgatgatgcg gatgcggaga
      541 gggaagcagg agcacaggtg atgcgtgaga ctgaacagca gatttaccgt cagctgactg
      601 acgaggtact ggccctgcga ttgtctgaaa acggctcaca actgcaccat tcataatcac
      661 atcgcataaa ccacagaccg gactgactcc ggaaaaacag aggcccgccc ccgggcctcc
      721 ccggattcat ccgtttccct gttcagcctg acagcacgcc ccggcggccg gatgacagac
      781 tccgcttcgg taagcaaagc ggtcttctgt gattccgcca gttgcggctt attcattact
      841 caacgtcaaa cgcccgaatt gaagccaaat catccagacc gctcagctcc tctttcattt
      901 cacgctgacg gcgataaatc tcatcgttgc gatcgacctg cgcctgcacc attgctgccg
      961 ccagttcttc cagttccggc atcgacagtt tcacctgctg attatcggca tcaccccacg
     1021 tcatcacgtc ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca
     1081 gtgagttggg gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct
     1141 cctgttctgt gcgccatgct tcaatttcct gacgtctggc ctctctggcc gcttccagca
     1201 tttctggtgt cacagtgaat ggggctatct caccccattt gccactttgc agttcctgcc
     1261 agattcgctg acccgtcggt gcgacatcat cagcggtggc tgtgtagggg actgcctggt
     1321 ccctgtcgtc aaaaaaaacg tcacagtcta ctgcgccact ttcggtataa cggggattaa
     1381 tgattttttt aatttccacg gtgcattcct cacgatgtgc gaataaaaag cccgggcatt
     1441 gcgccagaga
//
LOCUS       DAECNO010000248         1399 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.256, whole genome shotgun sequence.
ACCESSION   DAECNO010000248 DAECNO010000000
VERSION     DAECNO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.256"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..161)
                     /locus_tag="KKO08_004178"
     CDS             complement(<1..161)
                     /locus_tag="KKO08_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBD6320180.1"
                     /translation="MSQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLR
                     DIRKMLEEL"
     gene            complement(1001..>1399)
                     /locus_tag="KKO08_004179"
     CDS             complement(1001..>1399)
                     /locus_tag="KKO08_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBD6320181.1"
                     /translation="NAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLR
                     LMKNGYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGH
                     SSGSITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          391 a          317 c          296 g          395 t
ORIGIN      
        1 ttaagttctt cgagcatctt acgaatatca cgaaggacat ttcggtgctc cttgcctgtc
       61 agctcagcaa tttcaactga ggtcatggac acaagctggt cgaggttaat gatgctgatt
      121 tcttgttgtg gttgttgtgg ttgttgtggt tgttgagaca tcgcttgatt tcctttttgt
      181 ttgcaccctg aagtcgcacc cccactgacc tactcaaagt ccccaagcga agggagacca
      241 gcaggacaac aggatgcaaa cagaatgact ttgagattga atactatgat gatgacgatg
      301 tagctgtgcg gagctactgg gagggtctct ctaggagcca ctctagggtc tctctaggca
      361 tcaggctata cgaaactaca gggcatccta aagaatgacg tacagggcca tagaggacca
      421 tagagggcca tagagttagc ttctgggggt aatgcctaga ggagacccta gagagaccta
      481 tagagagacc ctagagaaga cctataggtt tggtgattga ttattataaa tatcatcacc
      541 ccttaaggta ttcccttaga gttaatcttt aattggtctt taattaaatc ttttatttaa
      601 tacttaaagt taatctctaa ttgtgtcttt aagttaactt taagtaatac tataagttaa
      661 tctttaattg tgtctttaag ttaactttaa gttaagcccc tgtagggact agagaccata
      721 attaagatga ttgtgtctta ttgctattca cttatcgact gacccagagc acacaggaag
      781 cattcacttt aagtagaacg cttggtgtct gtaattggtc atcccgcccc tgaaaggatg
      841 cgataagtta cggttagttg tcctagaaga gaaaactttt tgtgtccctt ctccctatag
      901 tgagtcgtat taattatccc ttgataagta gagaaaacta tgagtcaaaa acatgaccac
      961 acaggtctgt agtaagtgtc atcctagact atggtctcct ctaggtctcc ttataaacat
     1021 cagccatcac attgaccagc ctatcaacct cgacctcttt agcataacgg tcgtacgtaa
     1081 tagaccctga actatgcccc atgatcgcct gtgcatacgc caactcgacc ccatgttgtt
     1141 ttaactgggt cgcaactgta tgtctaaacg agtgaaacgc taagccgcta cggtctacga
     1201 ggactttagg caacaacgtc cgattaaacc atttactcac ctgctcaccg taaccgttct
     1261 tcatgagacg cagaccatcg aacaccatag cgtcatcacc gtcagcgcct ctacgggtct
     1321 ctacaagcgc cataaagtca gccaatataa aaccataggc cccatcgacc agaggcacac
     1381 agcggtcact atgagcgtt
//
LOCUS       DAECNO010000249         1377 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.257, whole genome shotgun sequence.
ACCESSION   DAECNO010000249 DAECNO010000000
VERSION     DAECNO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.257"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..250
                     /locus_tag="KKO08_004180"
                     /pseudo
     CDS             1..250
                     /locus_tag="KKO08_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            743..>1377
                     /locus_tag="KKO08_004181"
     CDS             743..>1377
                     /locus_tag="KKO08_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBD6320182.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          338 a          319 c          420 g          300 t
ORIGIN      
        1 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc
       61 caaacccttc ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag
      121 attatctggc ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc
      181 tggcctggat gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg
      241 gattgatatc gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa
      301 ttctccgcga cagaaatcac tgctcaaaat cggtgctgtt gaaaccggga tatgtgtctt
      361 gcggcgcagt gatatcgcaa ggcacaccga gcagggaatt ctggttatcg gtggggctgt
      421 tcgccagaaa aaatttgacc cgttgtcagt tgatctgccc gactggttgc cagaaacact
      481 ctggcatgag tgggtccagt tcaggcaggc attgcgaaaa ccgattcgaa cggagctggg
      541 cgctaacggg gcgatacggg aactggaaaa attccgtcag cagggtttta cacctgagca
      601 ggtgattcga cacagcatcg ccaatgaata ccagggtctg ttcgcgccga aaggtgttcg
      661 gcctgagacg ttgctccgac aggttaacac cgtctcgttg ccggacagtg cgatcccgct
      721 aggcttcagg gggtaacaga ccatgaaaaa tattgcgaca ggaggcgttc tggagcgtat
      781 ccgcagactg accccaccac atgtaaccgc cccattcaga acggttgcgg agtggcgcga
      841 gtggcaactt gctgaaggcc agaaacgttg cgaggagatc aaccgcctga atcgtcagtt
      901 gcgggtggaa aaaatcctcc gctccggcat ccagccgttg caccggaagt gttcgtttgc
      961 gaattaccgg gcgcagaacg acggtcagcg acatgcactg agtcaggcga aatccatagc
     1021 tgacgaactg atgacaggct gtacgaattt tgtgttcagc ggtaagcccg gcaccggaaa
     1081 aaatcatctt gcagccgcga ttggcaaccg gctgatggcg aaggggcgta gcgtgattat
     1141 cgtcaccgtg tccgatgtca tgagcgtgtt gcatgagagc tacgacaacg gcaaatccgg
     1201 tgaaaaattt ttacaggagc tttgtggtgt tgacctgctg gtcctggatg aaattggcat
     1261 gcagcgggat acgaaaaacg agcaggtggt actgaaccag attgttgatc gccggacggc
     1321 atcgttacgc ggtgtgggga tgctgacaaa tattaaccat gcagcgatga atacact
//
LOCUS       DAECNO010000250         1359 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.258, whole genome shotgun sequence.
ACCESSION   DAECNO010000250 DAECNO010000000
VERSION     DAECNO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.258"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..791
                     /locus_tag="KKO08_004182"
     CDS             <1..791
                     /locus_tag="KKO08_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBD6320183.1"
                     /translation="ATIRVMGFLVFPLIAYFLFLSIYLVGSWQPDLLTTQVEFNQNTL
                     HQIWISIPVMVFAFSHTPIISTFAIDRREKYGEHAMDKCKKIMKVAYLIICISVLFFV
                     FSCLLSIPSSYIEAAKEEGVTILSALSMLPNAPAWLSISGIIVAVVAMSKSFLGTYFG
                     VIEGATEVVKTTLQQVGVKKSRAFNHALSIMLVSLITFIVCCINPNAISMIYAISGPL
                     IAMILFIMPTLSTYLIPALKPWRSIGNLITLIVGILCVSVMFFS"
BASE COUNT          388 a          283 c          249 g          439 t
ORIGIN      
        1 atgccactat tcgggtaatg ggatttttgg tattcccatt gattgcctat ttcttatttc
       61 tttccattta ccttgtcggt agttggcaac ctgatctatt aacaacccag gtagagttca
      121 atcagaatac ccttcaccag atatggatat cgattcccgt gatggttttc gcctttagcc
      181 atacgcccat tatttctacg tttgccatag acagacgtga aaaatatggc gaacacgcta
      241 tggataaatg caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtactgt
      301 tctttgtctt tagctgcctg ctttctattc catcttcgta tattgaagcg gctaaagaag
      361 aaggggtcac cattttatcg gcgctttcta tgctgccgaa cgccccagca tggttgtcaa
      421 tttccgggat tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg
      481 gcgttattga aggtgccaca gaggtcgtca aaacaacact acagcaggtt ggtgtaaaga
      541 aaagtcgtgc atttaaccac gcactatcaa ttatgttggt atcgctgatt accttcattg
      601 tttgttgcat taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg
      661 ccatgatact tttcatcatg cctacgctgt caacgtatct catcccggcg cttaaaccct
      721 ggcgttccat cggaaatctg attacgctga tcgtgggtat cctgtgcgta tcggtaatgt
      781 tctttagcta atatccgttg tggatggttc aaaattgcta caattttgaa ccattccatt
      841 tttcataata ttcatttgtt attattccgc cctatcactt ctctcgtcat atcctagaat
      901 tatcagttag ttaaacacac aaagagatct gaacatctta aataagacta tttaagatgc
      961 ataacttaga ttcgcaagat atcttctggt cattcaaaaa caatttgcga tatagatatt
     1021 ctcataccat gagatagtta tctggaccac gttcgaccat gcaagattaa atcagtgaaa
     1081 cataatcata tttaataacg agaaatgcat ttttaaatat cttaatcaga attatagcaa
     1141 atacagatta atcgcaattt ttcccagcaa aattacaaaa tcaaggtgta ttgacatcat
     1201 caatcaccaa tgaaattatg caattatata cggataggga ggttcttaac atggggcttt
     1261 gtagtcgtta taaaagtctt acatgcaata gttgctcaat gcattgccaa ataatgccag
     1321 aagagtcacc gcgtttgcag tactgcgcaa actcgtgtt
//
LOCUS       DAECNO010000251         1356 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.259, whole genome shotgun sequence.
ACCESSION   DAECNO010000251 DAECNO010000000
VERSION     DAECNO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.259"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(41..100)
                     /locus_tag="KKO08_004183"
     CDS             complement(41..100)
                     /locus_tag="KKO08_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBD6320184.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="KKO08_004184"
     CDS             complement(339..1247)
                     /locus_tag="KKO08_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320185.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVEGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
     gene            complement(1281..>1356)
                     /locus_tag="KKO08_004185"
     CDS             complement(1281..>1356)
                     /locus_tag="KKO08_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBD6320186.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          274 a          361 c          326 g          395 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccctccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca actccaccga ttttgagaac gacagt
//
LOCUS       DAECNO010000252         1337 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.260, whole genome shotgun sequence.
ACCESSION   DAECNO010000252 DAECNO010000000
VERSION     DAECNO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.260"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..350
                     /locus_tag="KKO08_004186"
     CDS             <1..350
                     /locus_tag="KKO08_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320187.1"
                     /translation="PSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFD
                     ARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDG
                     VYYRPLQKLINGT"
     gene            374..918
                     /locus_tag="KKO08_004187"
                     /pseudo
     CDS             374..918
                     /locus_tag="KKO08_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            942..1337
                     /locus_tag="KKO08_004188"
                     /pseudo
     CDS             942..1337
                     /locus_tag="KKO08_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
BASE COUNT          378 a          291 c          352 g          316 t
ORIGIN      
        1 atccatccag gcctgttgtt gcagagcttg gcgatgattc aggctggcat tttttcagcc
       61 agagaaaccc ggataacagc atagtgttca ctgttaacgg acaggtaatt ccgttaaatt
      121 acggaaactt cgatgcccgc tataaatatc gaacagaggg ggtacaggat gtacggtatg
      181 gccatgaaat gtattacagc cccggcagta acaccgtttc gtggagattt tgcgcacctt
      241 cgggacacgg gctgtcaggg atggcgatat cggataccgg ccgtaactca gcggataacg
      301 ttgacggtgt gtattaccga ccactgcaaa aactgattaa tggcacctag tataacgtag
      361 cgagtattta acaatgttgc atttaaaaaa tattactacg ggtaatccga aaacagcaga
      421 acaatatcag atgacaaaac gatattcggt cacctggctt ttttcagaag acggaaaaaa
      481 ctggtatgaa gagctgaaga atttcgccag gacacaatta aaataactta caccggagac
      541 ggtcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
      601 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
      661 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
      721 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgttgct ggggctaatc
      781 agcgacgaag accgggaaaa actgaaagcg taccgcattt acgcgaaatc gctgcaggcg
      841 atggatttca gtgtcatcac tgataaaacc tcatacaacg ccattgaatg gcccgcttct
      901 ccggaaattt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
      961 gtgcgctgac tgatggcgca ggtatctcca tgtccggata ccatattatt ctgaaatccc
     1021 gggtaaacac cccggaagtg gtgatgcaca ctgttgctga tgtgatgaca ggaaacgatg
     1081 gtgaatactg tttccatgcg cggactggaa aatatggtgt gtatctgaaa caggactggc
     1141 acaacgagta caacgttggc gacattgctg tatatgagga ctcaaagccc ggtacgctga
     1201 atgactttct gattgctcct gatgagggcg acctgaaacc agatgtcgtc aaacgctttg
     1261 aggaaatggt ggcgcaggcg cagcagagca ggccaaaaat gacattgatg ctgcgctgac
     1321 cggcactctg aaaacgg
//
LOCUS       DAECNO010000253         1336 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.261, whole genome shotgun sequence.
ACCESSION   DAECNO010000253 DAECNO010000000
VERSION     DAECNO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.261"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..171)
                     /locus_tag="KKO08_004189"
     CDS             complement(<1..171)
                     /locus_tag="KKO08_004189"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320188.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1336
                     /locus_tag="KKO08_004190"
     CDS             308..>1336
                     /locus_tag="KKO08_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HBD6320189.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNST"
BASE COUNT          352 a          334 c          381 g          269 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag
     1321 cgccaacaac tccacg
//
LOCUS       DAECNO010000254         1279 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.262, whole genome shotgun sequence.
ACCESSION   DAECNO010000254 DAECNO010000000
VERSION     DAECNO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.262"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..1132
                     /locus_tag="KKO08_004191"
     CDS             join(<1..750,750..1132)
                     /locus_tag="KKO08_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBD6320190.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLSFRYLDHKTGETATETLTQRELVA
                     RLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLS
                     RDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          310 a          307 c          421 g          241 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatgcgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt
      961 ggcgaaagtg tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg
     1021 cgtgctgtgt ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg
     1081 gctgaagaaa aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct
     1141 aagtgtgccc gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac
     1201 catgcagaga gagaaaacac cggagtggag ggaaaagcag aaaagctcac gtggcataag
     1261 gcgtggacag cggtatcgt
//
LOCUS       DAECNO010000255         1272 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.263, whole genome shotgun sequence.
ACCESSION   DAECNO010000255 DAECNO010000000
VERSION     DAECNO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.263"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            482..>1272
                     /gene="uacT"
                     /locus_tag="KKO08_004192"
     CDS             482..>1272
                     /gene="uacT"
                     /locus_tag="KKO08_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBD6320191.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAV"
BASE COUNT          294 a          292 c          279 g          407 t
ORIGIN      
        1 gacagcctat acattccgca gcttcagcag cgataaactt attcatttgc atccctttca
       61 tttaatgagt tatgtcttct caaatttcgc gatgcaataa acgggcatat attaggtagc
      121 atgaccgttt taattataat tttgcgactg agttcaaatt attgcccctc aatctatgcc
      181 gctttcatta tttcaacatt gccgatcaaa agagcgctat ccagataaaa acgattaaat
      241 gcgagagtgc gacatgccag aatgattaat gaaatatcaa tatgagaaaa ttacagataa
      301 ttccattgtc aggttctctt gtcattttcg tggtttaagc acgattatgt gattgcctcc
      361 gcaattcctg tctctaactc cccttcctcg caaaaactgg cactccacga gcatgtgttt
      421 agacagtttc attaacgtaa acggttgctt tttattctgg cgggcgaaag gagaaacact
      481 gatgagcgcc atagattccc aacttccctc atcttctggg caagaccgcc caactgatga
      541 ggttgaccgc atattatcac caggaaagct gatcatactc ggtctgcaac acgtccttgt
      601 catgtacgca ggtgcagtcg ctgttcctct tatgattggt gaccgactgg gcctctcaaa
      661 agatgctatt gcgatgctca ttagctcgga tctcttttgc tgcgggatcg tcacattatt
      721 gcaatgtatc ggtatcggcc gctttatggg gatccgcctg ccggtgatta tgtcggtgac
      781 ctttgctgct gtaacaccaa tgatagccat tgggatgaac ccggatatcg gcctgctggg
      841 gatatttggt gccactatcg ccgcgggttt tatcaccaca ttattagcgc cacttattgg
      901 tcgcttgatg cctttattcc cgccactggt taccggtgtg gttattactt ctattgggct
      961 tagcatcatt caggtgggta ttgactgggc cgctggaggt aaagggaatc cgcaatatgg
     1021 taatcccgtt tatttaggta tctcctttgc cgtcttaatt tttatcttgc tcattactcg
     1081 ctatgcgaaa ggattaatgt ccaacgtcgc cgtattactg gggattgtat ttggcttttt
     1141 actttcgtgg atgatgaatg aagtcaattt atccgggcta catgatgcct cgtggtttgc
     1201 gattgtcacc ccgatgtcgt ttggtatgcc gattttcgat cccgtttcca ttctgaccat
     1261 gactgccgtg tt
//
LOCUS       DAECNO010000256         1259 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.264, whole genome shotgun sequence.
ACCESSION   DAECNO010000256 DAECNO010000000
VERSION     DAECNO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.264"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            34..435
                     /locus_tag="KKO08_004193"
     CDS             34..435
                     /locus_tag="KKO08_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBD6320192.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="KKO08_004194"
     CDS             447..818
                     /locus_tag="KKO08_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6320193.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="KKO08_004195"
     CDS             805..>1259
                     /locus_tag="KKO08_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBD6320194.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DAECNO010000257         1247 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.265, whole genome shotgun sequence.
ACCESSION   DAECNO010000257 DAECNO010000000
VERSION     DAECNO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.265"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..769
                     /locus_tag="KKO08_004196"
     CDS             <1..769
                     /locus_tag="KKO08_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBD6320195.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
     gene            790..1041
                     /locus_tag="KKO08_004197"
                     /pseudo
     CDS             790..1041
                     /locus_tag="KKO08_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            1097..>1247
                     /locus_tag="KKO08_004198"
     CDS             1097..>1247
                     /locus_tag="KKO08_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6320196.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVW"
BASE COUNT          391 a          282 c          290 g          284 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
     1021 ttactgactt acctgcaaag cgtgaaccgc cccgggaatc ctggagacta aacttcctga
     1081 gaaagaggta aacaggatga ctaaaaatac tcgtttttcc cccgaagtcc gtcaacgggc
     1141 agtccgtatg gttctggaaa gtcagggcga atatgactca caatgggcga caatttgttc
     1201 cattgcccca aagattggct gtacgccgga gactctgcgt gtctggg
//
LOCUS       DAECNO010000258         1208 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.266, whole genome shotgun sequence.
ACCESSION   DAECNO010000258 DAECNO010000000
VERSION     DAECNO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.266"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            163..>1208
                     /locus_tag="KKO08_004199"
     CDS             163..>1208
                     /locus_tag="KKO08_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBD6320197.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          392 a          276 c          191 g          349 t
ORIGIN      
        1 tcaaaaaaag atgaaccaaa tatacgaaaa aagcaaatat acttaccagg gtagaaagca
       61 tgatataaca ctcattccct tatggcaaat attctgaaac accctcctca ttaggagaga
      121 tacattaaat acaatttatt cacaatctaa ttccatatcc atatgcgaga aattaatatg
      181 ctcagaaata tttcatcctg tttatttcca catatcagca caattacatc ccccaaccat
      241 tatttgtccg aatgggatga ttgggagaaa caggggttac cggaagaaca gcgtactgag
      301 gcggtaagaa gacttcgtgc atgtcttacc tctaaggggc ataaactgga cctgcgagcc
      361 ttggcgcttt cctcgttacc tgtactccct gcttgcatta aaaagcttga tgtgagctgt
      421 aataaattaa ccatccttac tgatttacct gaaaatatta aagaacttat tgcaagagat
      481 aatttcttaa cacatatatc tgcattacca cattatctaa taactttgga tgtgtccgaa
      541 aatcaattag agaatctgcc gttattacca gacaccatca aatcactaag cgcagagtat
      601 aataggttat ccacactgcc ttcattaccc ttgaatttaa aaaaacttga ggttaggaac
      661 aacgaactgc aaactcttcc atctctgcct tctaatctta agatacttaa ggttgcgcac
      721 aaccatctta ctgaactgcc ccctttacct aggagactgc aacttctttt tgcatatagc
      781 aatagattaa gcaacttacc aaacatccaa gaaaatatta tcatgagaag atttttttat
      841 tttgaaaaca accaaataac tacaatcccg acaaatcttt ttcgtttaga tcctcatata
      901 actattgaga ttgcaaataa ccccttatca gatcaaactc tgctattctt aatacagcaa
      961 acttcggttc caaattttaa cgggcctcag tttcgtattt ccctgtcaga ccaaaacaga
     1021 ctgtttttac gccagatgtt gccgcaaaat ttacattcgc gccatatcag agtcatcact
     1081 gaaggggggc agaactttca gatcccccct cttcccgaaa ctgtggcagc ctggtttcct
     1141 gaagcagatc gtcgggaggt ttctacacaa tggacttctt tttccaccga ggagaattcc
     1201 cgggcatt
//
LOCUS       DAECNO010000259         1161 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.267, whole genome shotgun sequence.
ACCESSION   DAECNO010000259 DAECNO010000000
VERSION     DAECNO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.267"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            117..>1161
                     /gene="cadB"
                     /locus_tag="KKO08_004200"
     CDS             117..>1161
                     /gene="cadB"
                     /locus_tag="KKO08_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBD6320198.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASAILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          245 a          256 c          299 g          361 t
ORIGIN      
        1 tctttaggat taatcctttt ttcgtgagta atcttatcgc cagtttggtc tggtcaggaa
       61 atagttatac atcatgaccc ggactccaaa ttcaaaaatg aaattaggag aagagcatga
      121 gttctgccaa gaagatcggg ctatttgcct gtaccggtgt tgttgccggt aatatgatgg
      181 ggagcggtat tgcattatta cctgcgaacc tagcaagtat cggtggtatt gctatctggg
      241 gttggattat ctctattatt ggtgcaatgt cgctggcgta tgtatatgcc cgactggcaa
      301 caaaaaaccc gcaacaaggt ggcccaattg cttatgccgg agaaatttcc cctgcatttg
      361 gttttcagac aggtgttctt tattaccatg ctaactggat tggtaacctg gcgattggta
      421 ttaccgctgt atcttatctt tccaccttct tcccagtatt aaatgatcct gttccggcgg
      481 gtatcgcctg tattgctatc gtctgggtat ttacctttgt aaatatgctc ggcggtacct
      541 gggtaagccg tttaaccact attggtctgg tgctggttct tattcctgtg gtgatgactg
      601 ctattgttgg ctggcattgg tttgatgcgg caacttatgc agctaactgg aatactgcgg
      661 ataccactga tggtcatgcg atcattaaaa gtattctgct ctgcctgtgg gccttcgtgg
      721 gtgttgaatc cgcagcagta agtactggta tggttaaaaa cccgaaacgt accgttccgc
      781 tggcaaccat gctgggtact ggtttagcag gtattgttta tatcgctgcg actcaggtgc
      841 tttccggtat gtatccgtct tctgtaatgg cggcttccgg tgctccgttt gcaatcagtg
      901 cttcagctat cctcggtaac tgggctgcgc cgctggtttc tgcattcacc gcctttgcgt
      961 gcctgacttc tctgggctcc tggatgatgt tggtaggcca ggcaggtgta cgtgccgcta
     1021 acgacggtaa cttcccgaaa gtttatggtg aagtcgacag caacggtatt ccgaaaaaag
     1081 gtctgctgct ggctgcagtg aaaatgactg ccctgatgat cctcatcact ctgatgaact
     1141 ctgccggtgg taaagcatct g
//
LOCUS       DAECNO010000260         1160 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.268, whole genome shotgun sequence.
ACCESSION   DAECNO010000260 DAECNO010000000
VERSION     DAECNO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.268"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..387
                     /locus_tag="KKO08_004201"
     CDS             <1..387
                     /locus_tag="KKO08_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320199.1"
                     /translation="LVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(388..1032)
                     /locus_tag="KKO08_004202"
     CDS             complement(388..1032)
                     /locus_tag="KKO08_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6320200.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          296 a          263 c          277 g          324 t
ORIGIN      
        1 ctggtgctgg tggcaattct gattatgctg ccgactctcg gcatcggtga tatgaacaat
       61 ctctataaat ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta
      121 tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt
      181 attcgtaata agcctctggc aatgaccgtc gggatttggt gttttgcctt taccgcctgt
      241 gcctgtttga cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag
      301 ttggcgctga atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg
      361 ctggcgcgta aagcgaatag caaataatta ttgtggtcgg tgacgctgac tctggcagat
      421 ctgacccgtt tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt
      481 tgggtaatag acttggaagc ctggacgctc tgtgatccag tcttccagta cacattctaa
      541 acgaccagta tcaaggtact ccttgatctg ttcatagagc agatacccga taccagcacc
      601 catcaaaacg gcctccagtt cagcatcaac atcatccaga atgatatttc ctgcgacagc
      661 aatttccagc tcctttgcaa attgccagtg gaaaggtttg ccgctgggat aacgaaaaac
      721 aacacattga tgattaagca gatcatgagg atggcggggt ttgccatatc ttgcaaagta
      781 ctctggcatt gctgctacac ataatttcac tggtgggccg atcgccacgc tgatcatatc
      841 tttttcgacg atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat
      901 aagagaatcg tcagttgtga gctctacttt aatatcagga tattcacgtg tgaaaccaac
      961 cagtaaaggc attaaaaata tacgtgcagc tacccgggcg gcattgattt tgagcgttcc
     1021 tgtcggtgtc agacgaaaat cattcatctc atctaacatg atctgaattt cgtcgaaagc
     1081 tgggcgcaat cgttcataga gattagaacc cgcttccgtt aacgatacgc ttcgggttgt
     1141 ccgattgaat aagcgaattt
//
LOCUS       DAECNO010000261         1111 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.269, whole genome shotgun sequence.
ACCESSION   DAECNO010000261 DAECNO010000000
VERSION     DAECNO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.269"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..1019
                     /locus_tag="KKO08_004203"
                     /pseudo
     CDS             1..1019
                     /locus_tag="KKO08_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          272 c          259 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag c
//
LOCUS       DAECNO010000262         1076 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.270, whole genome shotgun sequence.
ACCESSION   DAECNO010000262 DAECNO010000000
VERSION     DAECNO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.270"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..1076
                     /locus_tag="KKO08_004204"
                     /pseudo
     CDS             1..1076
                     /locus_tag="KKO08_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          328 a          191 c          254 g          303 t
ORIGIN      
        1 taggcattca aggttctgaa gaacttttta gaaatatgat tggctattct cgtctgatgg
       61 ggcgaagtga agaagagatt aagcgagcac tgacagcatt atcacagatg gctggtaagg
      121 gtcagattat ggctgaagag cttaaggggc aactagctga agctgttcct ggtatggttc
      181 aggtattcgc taaagcaacc ggaaagactg aacaggaatt atttgatgct atgaagaagg
      241 gggctttaaa atctgccgat actcttcaga aggtcactca ggaactgaat aaacaaatca
      301 ccgctaaagg tggctggaaa gctatttcag aaagtacaca ggcacaacta gggaacttga
      361 aaaactcttg gaataccacg cttgatagta tcttcagagg ttctgaaaat ggtctgcaag
      421 attttacaag aagcttaacc aatcttttaa acgctcttgg tggaagtggt aagagtcttg
      481 gtgaatctct tggtagtctc atgacttcta tgtcacatgg tgtcgattca ttgaccgata
      541 ttagttacag ggtaagggcg ttctttgatg aggtgacatt agcttaccgt ccactaaatg
      601 acactcagag gaaaattgtt gatggtttat ctggtgggtt aattgatggt ctgaagatgc
      661 ttgctgtggc tgttactgct caaaaggctg caaatgtagc aggaggcttg ttaaaccttg
      721 caaacgctgt tacttcattc ggtgacgctg ttaataaagc taaagctggc aaggctggtt
      781 cttcaggtgg tgcgctaaat aaagtcggta gagttgtcct ttatgctgag ttggtaaatt
      841 ttgctcttga taatttaaac cctgaagcag ctaagaatcc gaacttacat caatcaggta
      901 ttggcacaga aacaaacttc aaccctgaat acaaaggtga ttcatctaag ccaatagttc
      961 tatcgctgac tggtttaatg gtgcaactaa taaaattgct aacttccaag aaccgatgaa
     1021 taacttcgta aataacacac aatcaactcc atcagctatc acaggtgaca tggtta
//
LOCUS       DAECNO010000263         1063 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.271, whole genome shotgun sequence.
ACCESSION   DAECNO010000263 DAECNO010000000
VERSION     DAECNO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.271"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(567..746)
                     /locus_tag="KKO08_004205"
                     /pseudo
     CDS             complement(567..746)
                     /locus_tag="KKO08_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            840..>1063
                     /locus_tag="KKO08_004206"
     CDS             840..>1063
                     /locus_tag="KKO08_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6320201.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          284 a          193 c          277 g          309 t
ORIGIN      
        1 gggtagtcac tatatttgcg actgagctgg tcgctatttt taaccaggct gaaaaattga
       61 gtcaatatat cgaaagcggt agttggcggg cataagtatg taatgatgat gctgcactgg
      121 tgttgaatat tattcacgcc aaactttcct cattgagcaa tgtcgtcgca agtggcctca
      181 atgattttag accattatca gaggtccgga gcggcgggaa gagatttatt gctgtggaat
      241 aattgtggta ctgatgatta tttatatccc ggattggtag ggaaaagctt ttttcatgca
      301 ctatttttgc aaatatttct atatgggaag tggttttgtt acagattaaa tttataaaga
      361 aatgcatttg gggtgttggg ttgaagagta gctggtagat gttattgtgc cagcaggggc
      421 aggggaatgt gccgtatcga tatgctttgg caattgcgtg tgccgcagat ggagatgaaa
      481 tgccaataaa aaagcctgag ttcgatgagg gacgaactca ggctggtgtt atgttttaca
      541 gtaacacgcc tcggcgcgtt ggctgattag aactggtaga cgataccaac tgccagttgg
      601 tcgtcggtgt tcagtccatg agcgagaaca taatcactct tatcaagcag gttgatctga
      661 taagcggtat atatgttcat attcttattg aagtaatacc aggtaccgat ttcaatatag
      721 ttcagacgat cagaatcttt gtaacctgta gactggcccc ctgaatctcc agacaaccaa
      781 tatcacttat ttaagtgata gtcttaatac tagtttttag actagtcatt ggagagcaga
      841 tgattgatgt cttaggaccg gagaaacgca gacggcgtac tacacaggaa aagatcgcta
      901 ttgttcagca gagttttgaa ccgggaatga cggtctccct tgttgcccgg caacacggtg
      961 tggcagccag ccagttattt ctctggcgca agcaatacca ggagggaagt cttactgctg
     1021 tggccgcagg agaacaggtc gttcctgcct ctgaacttgc tgc
//
LOCUS       DAECNO010000264         1057 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.272, whole genome shotgun sequence.
ACCESSION   DAECNO010000264 DAECNO010000000
VERSION     DAECNO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.272"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            26..>1057
                     /locus_tag="KKO08_004207"
     CDS             26..>1057
                     /locus_tag="KKO08_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBD6320202.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAE"
BASE COUNT          324 a          198 c          278 g          257 t
ORIGIN      
        1 tctggagatt cagggggcca gtctattgtc tataaccacg aaccgttatg catcaggtta
       61 tgccacactg actgaagcag taagtgcaca ggatgaacgg aacagaaaaa gagataagaa
      121 tagtcacgat ggctctacga tatccttatc ccagcccctt ggtaatatcg ggaatttgaa
      181 cttcaatacc acgaggtata attcttcccg gggcacagga aacacgcggt ctaccagcct
      241 gagttatagt acggtatgga gaggcataac attctccatt aactgggcaa aaaacgactt
      301 gctgacatcg cataaatgga aagtagatcg gaaattaagc gtgggtattt cagtgccatt
      361 atcgctgggt gacgaaaatc aaatttatgc gtcttcacag atgagtcgtt cgggagagca
      421 gggtaataac tatcaggttt cattatcagg tcagaatagt ggtggtgtat ggtgggatgt
      481 agcgacaaat ataactaacg ctcatcagtc acaaccgaag agcacgatga acattgtgca
      541 agttggcaaa aatggatcat atggtcagtt tagtagccat tacagctcca gcgaaaacat
      601 gaaacagcta ggtgcaaatt taagcggtgg gatacttatt acaagggatg gacttacatt
      661 tggtcaaaat gtggatggga cattagccct tatagaggca cccggagcaa cgggggttaa
      721 cgttaatggc tggccgggac tgagtactga ttttcgtggg tatgcaatac tgccagtgca
      781 gccataccga agagacgatg ttattctgga tgaaaaaacg ataggtaaga attacgactt
      841 accacaaact agtcaattgg ttgtaccgac agctggagcc gttgtgcctg cgacattggc
      901 agtgaagagc ggggataaag gattagtaac cttaaaacag aaagagggta aacccgtccc
      961 cttcggcgct gtgattagtt atagcaaaga cactgaaaat atggccggca ttgtaggtga
     1021 agatggtatt gcttatgttt ctggcttgag tgcggaa
//
LOCUS       DAECNO010000265         1021 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.273, whole genome shotgun sequence.
ACCESSION   DAECNO010000265 DAECNO010000000
VERSION     DAECNO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.273"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(50..190)
                     /locus_tag="KKO08_004208"
     CDS             complement(50..190)
                     /locus_tag="KKO08_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320203.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="KKO08_004209"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="KKO08_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBD6320204.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          206 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
     1021 t
//
LOCUS       DAECNO010000266         1003 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.274, whole genome shotgun sequence.
ACCESSION   DAECNO010000266 DAECNO010000000
VERSION     DAECNO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.274"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..87
                     /locus_tag="KKO08_004210"
     CDS             <1..87
                     /locus_tag="KKO08_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBD6320205.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            102..524
                     /locus_tag="KKO08_004211"
     CDS             102..524
                     /locus_tag="KKO08_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBD6320206.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            582..938
                     /locus_tag="KKO08_004212"
     CDS             582..938
                     /locus_tag="KKO08_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320207.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          288 a          201 c          276 g          238 t
ORIGIN      
        1 cgcatggtca tgaacggcgg gcgctgggtt aattttaact gggagagctg gcgtccgaat
       61 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
      121 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
      181 tcacgattcg gcagttgtcc gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
      241 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
      301 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
      361 aaccggttgt tgttgatccg gaccttatct ggtcattacc tgacggagaa atacgccgct
      421 acgacaggcg cctgaatata atctgtcgcg agtgccggaa gagcgaagct atgcagcgtg
      481 tactggcttt ctatcagggt aattttcagg aggcgatact gtgaatgaaa ttagctatca
      541 ggcttcaatt accgctggca ttcgcatcaa aggagaggag catggaaata aaaccagaag
      601 atgagttaag caatatcgtt ttatttccga taaaagagga tgaccctcgt aatcaggtta
      661 attttcttta taagccatcg gaaagaccat attgccatca cgcctctgta cgggttgacg
      721 aaaaagagcg tcaggtccgc tgtaaaatct gcggtgcggt tgtggagccg tttgactgga
      781 tgctctctgt ggcaaaaaga gaaaccagac tggcagatga tgtaaggcta ttgcgccagg
      841 aggaacagga aaggcggaga aatatagaaa agctgataca gattgagcgt aacgcgaaag
      901 cgcggatacg cagggcgaca aaatccagaa ctgaataatt aaatttagca ctgttaaaaa
      961 tttaatcctt aaccggaggg atttctgcac cctcaaatca tca
//
LOCUS       DAECNO010000267          983 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.275, whole genome shotgun sequence.
ACCESSION   DAECNO010000267 DAECNO010000000
VERSION     DAECNO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.275"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KKO08_004213"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="KKO08_004213"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBD6320208.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAECNO010000268          973 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.276, whole genome shotgun sequence.
ACCESSION   DAECNO010000268 DAECNO010000000
VERSION     DAECNO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.276"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(27..102)
                     /locus_tag="KKO08_004214"
     tRNA            complement(27..102)
                     /locus_tag="KKO08_004214"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Thr,seq:tgt)
     gene            complement(105..181)
                     /locus_tag="KKO08_004215"
     tRNA            complement(105..181)
                     /locus_tag="KKO08_004215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Arg,seq:tct)
     gene            complement(183..259)
                     /locus_tag="KKO08_004216"
     tRNA            complement(183..259)
                     /locus_tag="KKO08_004216"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Ile,seq:cat)
     gene            complement(468..965)
                     /locus_tag="KKO08_004217"
     CDS             complement(468..965)
                     /locus_tag="KKO08_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBD6320209.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          250 a          249 c          226 g          248 t
ORIGIN      
        1 aatagccatt atacgatgcc cgcatatggt gccgactacc ggaatcgaac tggtgaccta
       61 ctgattacaa gtcagttgct ctgcctgctg agctaagtcg gcgctggccc accaccgagg
      121 actcgaacct cgcaccgtca acttagaagg ttgatgctct atccggatga gctagtggtg
      181 gttggtggcc cttgctggac ttgaaccagc gacctggcga ttatgagtcg ctcgctctga
      241 ccaactgagc taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact
      301 catctgacca ccagcgcgtt taacgccctg tgccattttt caggcacaaa aaaacccgca
      361 taaagcgggt tctttcaagt gtccatgtct gctattcgcc tcgcgataca gctttgcgaa
      421 gcttaccgga attgaagcag tttatgtgca aaaatgcaag aactttttta aagctgcatc
      481 aacctttcca ccagtttatc tctgcgaaca acaaaccaac cattggctct cgccagttcc
      541 agccatgact caagggaaat aacaatatca tcatcccgta actgaattgt ggaaacagtg
      601 acaccgcctc tctgataaca gagaactcgc gtgtcgtaac ttttctggca tgaaactggc
      661 gttgacggat ccctttgact gaaatagcag tcttccagtt tttcgaacac atcccacgcc
      721 tgatcggttt cgagcatttt gccgtggcgt gctgcgcctc gttctgtcca gaggatgagg
      781 gagcgggctt tgggtgaaac tggattttgt gagtagttta aagcgacccg caattcttta
      841 aggtcattac caacaacttt gaaaaagtgt ttcccttcaa cgaagcgtac tttgttctca
      901 tgatgattct ggcgaatacg caccggctca gtgccgtaaa gctgcgccaa aagttcggtg
      961 gtaataacag gaa
//
LOCUS       DAECNO010000269          933 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.277, whole genome shotgun sequence.
ACCESSION   DAECNO010000269 DAECNO010000000
VERSION     DAECNO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.277"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
BASE COUNT          245 a          180 c          217 g          291 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc atctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAECNO010000270          883 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.278, whole genome shotgun sequence.
ACCESSION   DAECNO010000270 DAECNO010000000
VERSION     DAECNO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.278"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..463)
                     /locus_tag="KKO08_004218"
     rRNA            complement(<1..463)
                     /locus_tag="KKO08_004218"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          197 a          245 c          188 g          253 t
ORIGIN      
        1 actttactcc cttcctcccc gctgaaagta ctttacaacc cgaaggcctt cttcatacac
       61 gcggcatggc tgcatcaggc ttgcgcccat tgtgcaatat tccccactgc tgcctcccgt
      121 aggagtctgg accgtgtctc agttccagtg tggctggtca tcctctcaga ccagctaggg
      181 atcgtcgcct aggtgagccg ttaccccacc tactagctaa tcccatctgg gcacatccga
      241 tagcaagagg cccgaaggtc cccctctttg gtcttgcgac gttatgcggt attagctacc
      301 gtttccagta gttatccccc tccatcgggc agtttcccag acattactca cccgtccgcc
      361 actcgtcagc gaaacagcaa gctgtttcct gttaccgttc gacttgcatg tgttaggcct
      421 gccgccagcg ttcaatctga gccatgatca aactcttcaa tttaaaagtt tgatgctcaa
      481 agaattaaac ttcgtaatga attacgtgtt cactcttgag acttggtatt catttatcgt
      541 cttgcgacgt taagaatccg tatcttcgag tgcccacaca gattgtctga taaattgtta
      601 aagagcagta ccgcttcgct ttttctcagc ggcgcggggt gtgcataata cgctttcccg
      661 ctacagagtc aagcatttat ttttgctttc tctgccggaa ttctcaggag aaccccgctg
      721 acccggcggc gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag ggagttattc
      781 cggcctgaca agcgaaaaat ataaaaactt tatcgttcgc tcacttttca ggcaaaacat
      841 cttaaatata gtcttttccg tctaacttat agacaaaaac gag
//
LOCUS       DAECNO010000271          866 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.279, whole genome shotgun sequence.
ACCESSION   DAECNO010000271 DAECNO010000000
VERSION     DAECNO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.279"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>866
                     /locus_tag="KKO08_004219"
     CDS             <1..>866
                     /locus_tag="KKO08_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBD6320210.1"
                     /translation="LLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAGEA
                     KTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGL
                     LTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLA
                     ADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVL
                     TSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKA
                     LKRALFLSAFAA"
BASE COUNT          165 a          293 c          232 g          176 t
ORIGIN      
        1 tactgctggt tgttgatcag ccagctacca tcggtgcgtt acctgtcgcc gttgcccgct
       61 cagaaggagt ccttgtcgga tacctccctg gactggccat gcgccgcata gccgacttac
      121 acgccggtga agctaaaact gatgctcgtg acgctgccat cattgccgaa gctgcccgta
      181 ccctgcctca cgcgctacgc acgctgaaac tggctgacga gcaaatcgcc gaactctcca
      241 tgctctgcgg cttcgatgat gatcttgccg cacagacaac gcaggccagc aaccgtatcc
      301 gcggccttct gacccagata catccggcac cggagcgcgt tctcggtccg agacttgagc
      361 acccggcggt actcgatctt ctccagcgat atccctcacc agaaaaactc gcttcgctgg
      421 gtgagaagaa gctggcagcc cagctctgca aacttgcgcc tcgtctgggt aaacgccttg
      481 cagcagacat agctcaggca ctggccgaac aaaccgtcgt cgttcccggc acgaatgccg
      541 ctgccgtagt actgccacgt ctggcactcc agctcatcac gctgcgtaag caaagagacg
      601 aggtggcgct tgaggtagaa cagcgagttc ttgctcaccc tctttacccg gtcctgacca
      661 gtatgcccgg agtcggtgtc aggaccgcag ccagactcct caccgaggtc gcctgccgcg
      721 ccttcgcctc tgccgcacat ctcgctgctt atgctggcct tgcgccggta actcggcgat
      781 ccggctcgtc aatacgcggt gagcatccct cgcgacgggg taataaagct ctcaaacggg
      841 cgttgttcct gtcggccttc gccgcg
//
LOCUS       DAECNO010000272          831 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.280, whole genome shotgun sequence.
ACCESSION   DAECNO010000272 DAECNO010000000
VERSION     DAECNO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.280"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..326
                     /locus_tag="KKO08_004220"
     CDS             <1..326
                     /locus_tag="KKO08_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBD6320211.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="KKO08_004221"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="KKO08_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBD6320212.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          154 g          241 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAECNO010000273          812 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.281, whole genome shotgun sequence.
ACCESSION   DAECNO010000273 DAECNO010000000
VERSION     DAECNO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.281"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            79..351
                     /locus_tag="KKO08_004222"
     CDS             79..351
                     /locus_tag="KKO08_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320213.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            351..656
                     /locus_tag="KKO08_004223"
     CDS             351..656
                     /locus_tag="KKO08_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320214.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            656..>812
                     /locus_tag="KKO08_004224"
     CDS             656..>812
                     /locus_tag="KKO08_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBD6320215.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQAC"
BASE COUNT          262 a          181 c          195 g          174 t
ORIGIN      
        1 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca gaaagtaaat
       61 gaaacagatg ctgaatacct ggtgcgtaaa ttcgatgagc ttgaagctaa atgtgcagca
      121 caggaaaaca aagtaatacc agtgtcaact gaactgccac cagcaaatga aagtgttttg
      181 ttattcgatg ctaacggaga aggctggcta attggctggc gttctctctg gtacacctgg
      241 ggacaaaaag aaaccggaga atggcagtgg acatttcagg tcggggacct tgaaaacgtc
      301 aatatcactc actgggcagt aatgccaaaa gcaccggagg ctggagcata atgaccactt
      361 ttaccgacaa agaactgatt aaagaaatta aagagcgtat cagcagcctt gacgtgcgag
      421 acgatattga gcgccgtgct tatgaaatcg cactcctatc tctggaagta gaaccagatg
      481 aacgcgaagc ttatgaatta ttcatggaaa agcgttttgg tgacttagta gatcgtcgga
      541 gagcaaaaaa cggcgataac gaatacatgg catgggatat gactctcggt tggatcgtct
      601 ggcagcaacg agctggtatc catttctcaa caatgtcaca gcaagaggtg aaataatgga
      661 gccatacagc ctcacactcg atgaggcctg tcattttctc aagatatcca gaccgactgc
      721 cattaactgg atacgcacag ggcgtcttca ggcaacacgc aaagatccca ctaagaataa
      781 atctccttac ctcacaacac gacaagcctg ca
//
LOCUS       DAECNO010000274          801 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.282, whole genome shotgun sequence.
ACCESSION   DAECNO010000274 DAECNO010000000
VERSION     DAECNO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.282"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>801
                     /locus_tag="KKO08_004225"
     CDS             <1..>801
                     /locus_tag="KKO08_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBD6320216.1"
                     /translation="RRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLR
                     RQAELDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGF
                     EFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSP
                     VEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPK
                     PDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          194 a          192 c          248 g          167 t
ORIGIN      
        1 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
       61 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      121 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      181 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      241 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      301 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      361 ctgtgatcgt gaggcactgc actgggcggt gactaccggc ggcttcaaca gtgaaacagt
      421 acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgatcttc cgtcgtctcc
      481 agtggagtgg ctgacggata atggttcatg ctaccgggct aatgaaacac gccagttcgc
      541 ccggatgttg ggacttgaac cgaagaacac ggcggtgcgg agtccggaga gtaacggaat
      601 agcagagagc ttcgtgaaaa cgataaagcg tgactacatc agtatcatgc ccaaaccaga
      661 cgggttaacg gcagcaaaga accttgcaga ggcgttcgag cattataacg aatggcatcc
      721 gcatagtgcg ctgggttatc gctcgccacg ggaatatctg cggcagcggg cttgtaatgg
      781 gttaagtgat aacagatgtc t
//
LOCUS       DAECNO010000275          799 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.283, whole genome shotgun sequence.
ACCESSION   DAECNO010000275 DAECNO010000000
VERSION     DAECNO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.283"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>799
                     /locus_tag="KKO08_004226"
     CDS             <1..>799
                     /locus_tag="KKO08_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320217.1"
                     /translation="QKLSYENILPLNNSLIKNWNIQKFSSDYSEIEHLNVINGMGVTL
                     GDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYVEELYELASSVIDELHYMPFHIQKL
                     PCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVKLDDVNHNELSNKWLGNIDLGAPVF
                     ENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREGKTVLGFVDVNHKNYINISKYSPHT
                     KDFIALIKYASNVYTCDSSALHIAAGFGVPTECVFNTIPPELRTKYY"
BASE COUNT          268 a          136 c          148 g          247 t
ORIGIN      
        1 gtcagaagct aagctatgaa aatattttac cattaaacaa tagtcttata aaaaactgga
       61 atatacaaaa attctcaagt gattactctg aaatagaaca cctaaatgtg atcaatggca
      121 tgggagtgac attaggtgat tctatcgtcg ggatttctgt gctgtcagca atcaaaaaaa
      181 agaatcccaa gattaaaatt aagcttattc gaccggagac tgcaccgtca tatgttgagg
      241 aactgtatga attagcatcc tcggttatag atgagcttca ttacatgcct tttcatattc
      301 agaaattgcc ttgtactgca ttgaatattg atgtgggaaa tcagctatat tctccagatt
      361 tccatattat ggaaatgcat gattatttta tccaaggtct tggcgtgaaa cttgatgatg
      421 ttaaccataa tgagctatct aataaatggt taggcaatat tgatctcgga gcgccagttt
      481 ttgaaaacta cacacttttt gcacctattg ctagcacaaa aataagaagt ataccgtcga
      541 agttctatta tgacatagtt gatatgcttt ctaggcgcga aggaaaaacg gtactaggtt
      601 tcgtggatgt gaatcataaa aattatatta acatatcaaa gtattcaccc catacgaaag
      661 atttcatagc gctaattaag tatgctagca acgtttatac ttgtgactcc tctgcattgc
      721 atatagccgc tggatttggt gtacctactg aatgcgtgtt taatacaata ccgccagaat
      781 taagaacaaa gtattacag
//
LOCUS       DAECNO010000276          791 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.284, whole genome shotgun sequence.
ACCESSION   DAECNO010000276 DAECNO010000000
VERSION     DAECNO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.284"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(137..760)
                     /locus_tag="KKO08_004227"
     CDS             complement(137..760)
                     /locus_tag="KKO08_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBD6320218.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          155 a          253 c          189 g          194 t
ORIGIN      
        1 gaggcccgcc cccgggcctc cccggattca tccgtttctc tgttcagcct gaccgcacac
       61 cccggcggcc ggatgacaga ctccgcctcg gtaagcaaag cggtcttctg tgattccgcc
      121 agttgcggct tattcattac ttaacgtcaa acgcccgaat tgaagccaaa tcatccagac
      181 tgctcagctc ctctttcatc tcccgctgac ggcgataaat ctcatcgttg cgatcgacct
      241 gcgcctgcac cattgctgcc gccagttctt ccagttccgg catcgacagt ttcacctgct
      301 gattatccgc atcactccac accatatgtg tttgtgccgc gacagatttt gccagcatga
      361 ccaccgggga caggcgggca actgacgagg cgtcagcgtt ccacgtgcgg ccgttccatt
      421 caaacgtgaa cggcttcgcc tcctgttctg tgcgccatgc ttcaatttcc tgacgtctgg
      481 cctctctggc cgcttccagc atttctggtg tcacagtgaa tggggctatc tcaccccatt
      541 tgccactttg cagttcctgc cagatttgct gacccgtcgg tgcaacatca tcagcggtgg
      601 ctgtgtaggg aactgcctgg tccctgtcgt caaaaaaaac gtcacagtct actgcgccac
      661 tttcggtata acggggatta atgatttttt taatttccac ggtgcattcc tcacgatgtg
      721 cgaataaaaa gcccgggcat tgcgccagag acatgagcat ccggcacccc ggacagggcg
      781 caatatgacc c
//
LOCUS       DAECNO010000277          759 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.285, whole genome shotgun sequence.
ACCESSION   DAECNO010000277 DAECNO010000000
VERSION     DAECNO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.285"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>759
                     /locus_tag="KKO08_004228"
     CDS             <1..>759
                     /locus_tag="KKO08_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBD6320219.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAECNO010000278          721 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.286, whole genome shotgun sequence.
ACCESSION   DAECNO010000278 DAECNO010000000
VERSION     DAECNO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.286"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            173..238
                     /locus_tag="KKO08_004229"
                     /pseudo
     CDS             173..238
                     /locus_tag="KKO08_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            466..714
                     /locus_tag="KKO08_004230"
     CDS             466..714
                     /locus_tag="KKO08_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBD6320220.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          228 a          154 c          169 g          170 t
ORIGIN      
        1 cgagagtggt aaaggagagg ccctggaaat accccacagc cccgaaaaag agccagtcag
       61 ttgcttaact gactggcatt accctataga tgcacaaaaa acgggcaaaa ctacctggtt
      121 cgcaaaactg cgtctaaagt taaactggga cctcgcgagc aagggtgaga cgatggcgct
      181 ttacacaatt ggtgaagtgg cgttgctttg tgatattaat cctgtcacgt tacgcgcgtg
      241 acgcaacatt tttaaaaatc gacgaagatc gtccaggagc gccgtttttc aagggttgga
      301 tagatgattc gtttaccatg tcgacgaact tcgacgaatt tcgccgataa aaagtgtccc
      361 atacatgccc cattgatgcc ccataaacaa tatcaggaat tgacactggt tatccataca
      421 gataaaaata acacctgtac ataactacag ttacaggagg gtgttatgcg tgttgagatc
      481 tgtatcgcta aagaaaaaat cactaaaatg ccaaacggtg ctgtggatgc gttaaaggaa
      541 gaattaaccc gacgcatcag taaacgttat gacgatgtag aggtgatcgt aaaagccacc
      601 agcaacgatg gcctttctgt tacacgcacc gcagataagg attctgcaaa aacatttgtt
      661 caggagactc tgaaagatac ctgggagtct gctgacgagt ggtttgttca ctaataaaca
      721 c
//
LOCUS       DAECNO010000279          693 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.287, whole genome shotgun sequence.
ACCESSION   DAECNO010000279 DAECNO010000000
VERSION     DAECNO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.287"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..525
                     /locus_tag="KKO08_004231"
                     /pseudo
     CDS             1..525
                     /locus_tag="KKO08_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>693
                     /locus_tag="KKO08_004232"
     CDS             589..>693
                     /locus_tag="KKO08_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBD6320221.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          193 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acc
//
LOCUS       DAECNO010000280          687 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.288, whole genome shotgun sequence.
ACCESSION   DAECNO010000280 DAECNO010000000
VERSION     DAECNO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.288"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..667)
                     /locus_tag="KKO08_004233"
     CDS             complement(<1..667)
                     /locus_tag="KKO08_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBD6320222.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          185 a          173 c          154 g          175 t
ORIGIN      
        1 ttgccgcatc atcatcagac tccggaacgc cctcatacac atgtttaaaa ccatccggat
       61 cacggcgtcg cgccgcgtcg ataaccttaa gcatggtgtc agacagaaac ggattttcgt
      121 catagttaat tttgcggatg agagtgcctt cgggcggatc aacaacgaag ttgcgccaga
      181 cgaaatcagt aacaagtccg gggttgaata tgaaccagca ttccgaaccc tctttacgga
      241 tcgttggctc cagaattttc cactggtatt ccgtcagtgc gtgggcttct tccagccaca
      301 acatatcgat cccctccagt gacttaattt cttcgatgtt gcgccataat ccataaaaaa
      361 caaattccga gccagtaacc cggttaatga ttttgttgtt cagaatacgg aaacggtgcc
      421 gcagaccaaa cctgtctatc tgaattttga gcagggtata caccgactct tcgattttat
      481 tctggatctg acgggcacaa caaaaacgca gggtgtattt attcgacaga aatatggcaa
      541 tgccagcggc atcccatgat tttgacgatg accgaccacc ataaagcact ttgttacgtg
      601 cccgcatagt ccagaaacta cgtaaagccg gattcagcgt cggtttggat gtcagagtag
      661 aagtcattga ggtcacgctc tccattg
//
LOCUS       DAECNO010000281          655 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.290, whole genome shotgun sequence.
ACCESSION   DAECNO010000281 DAECNO010000000
VERSION     DAECNO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.290"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..491
                     /locus_tag="KKO08_004234"
     CDS             <1..491
                     /locus_tag="KKO08_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBD6320223.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EM"
     gene            553..>655
                     /locus_tag="KKO08_004235"
     CDS             553..>655
                     /locus_tag="KKO08_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBD6320224.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRL"
BASE COUNT          166 a          142 c          161 g          186 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc
      241 ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtg aggtagcctg agtttaacgg acactccttc ctgaaataga atggcatcag
      541 aaggagctaa taatgagcag aaaaacccga cgttactcta aagagttcaa agccgaagct
      601 gtcagaacgg ttcttgaaaa tcaactttcg atcagtgaag acgcttcccg attat
//
LOCUS       DAECNO010000282          644 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.291, whole genome shotgun sequence.
ACCESSION   DAECNO010000282 DAECNO010000000
VERSION     DAECNO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.291"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(230..>644)
                     /locus_tag="KKO08_004236"
     CDS             complement(230..>644)
                     /locus_tag="KKO08_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBD6320225.1"
                     /translation="FDCVARPWLTEAGSRSARGIWEIEFNHKLLRYIYGLTNQFTTYS
                     LRDCGSLRNPRTIRLYESLAQFKSSGLWVTTHAWLNDRFLLPESQQKNLAELKRSFLD
                     PALKQINEKTPLLAKYSIDDSGKFLFSIIDKQNPV"
BASE COUNT          150 a          157 c          180 g          157 t
ORIGIN      
        1 gggcgccgga gccgtgtggg aatccgccca ccttgccggg cgccggtgcc gtgtgggaat
       61 ccgcccacct tgccgggcgc cggagccgtg tgggaatccg cccaccttgc cgggcgccgg
      121 tgccgtgtgg gaatccgccc accttgccgg gcgccggtgc tgtgtggaaa ttcgcccatc
      181 ttcatgacaa aaatttgtca aatgacaggc tcatgtgtgc tgatttatgt cagacgggat
      241 tttgcttatc aattattgag aacagaaatt ttcctgaatc atcaatacta tacttagcaa
      301 gtaaaggtgt tttctcattt atctgcttga gtgcaggatc aaggaaagat cgtttcaact
      361 ctgccaagtt cttctgttgg gattccggca aaaggaaacg gtcatttaac caagcatgag
      421 tagtaaccca taagcctgaa gatttgaatt gagcaagact ttcataaagg cggatcgtcc
      481 gtggatttcg aagactgcca caatcgcgga gcgagtaggt ggtgaactgg ttcgtcaggc
      541 cgtaaatgta ccgcaggagt ttatggttaa attcgatttc ccagatacca cgagctgatc
      601 ggctgccagc ctctgttagc caggggcgcg cgacgcagtc aaaa
//
LOCUS       DAECNO010000283          635 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.292, whole genome shotgun sequence.
ACCESSION   DAECNO010000283 DAECNO010000000
VERSION     DAECNO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.292"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..>635)
                     /gene="rfbG"
                     /locus_tag="KKO08_004237"
     CDS             complement(<1..>635)
                     /gene="rfbG"
                     /locus_tag="KKO08_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBD6320226.1"
                     /translation="RLIKDVDLENYDYICFSDQDDIWYKDKIKNAIDCLVFNNANCYS
                     SNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIEFKKFIINNKNAA
                     QDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFKAKYKRLGLVRNK
                     WYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKADKIYIL"
BASE COUNT          201 a          127 c           75 g          232 t
ORIGIN      
        1 ataaaatata aattttatca gcctttttcc gccttaactt ccaaaaactt aaagccaaga
       61 tcaaattgcc tatataacct ttacccaatt gattattcac aaagctatca tcagctaagg
      121 cattagctat tttagttact tcttttcgat accacttgtt tctcaccaaa ccaagccgtt
      181 tgtattttgc tttaaaacta atgtttgctc ccacttggtt gttttcatgc tgtctgtaca
      241 gcattgttgg ttttctatca atataccacg aataatttct cgtccgcgca aatgaatata
      301 aaaaccaatc atgcagacag atatcttgtg cagcattttt attatttatg atgaacttct
      361 taaattcaat aagcgtttct ttttttataa catatgtaca ccccggacct gctgcttcaa
      421 aaaaataatc gaattgagtt tgcgactgag ccttatcaac taaatttttt cgccctgatg
      481 gataatatgc aattacattg ctggaataac aattcgcatt attaaatacc aagcagtcaa
      541 ttgcattttt tattttatct ttataccaaa tatcgtcctg atcactaaaa cagatataat
      601 catagttttc aagatcgaca tctttaatta atcta
//
LOCUS       DAECNO010000284          630 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.293, whole genome shotgun sequence.
ACCESSION   DAECNO010000284 DAECNO010000000
VERSION     DAECNO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.293"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..112)
                     /locus_tag="KKO08_004238"
     CDS             complement(<1..112)
                     /locus_tag="KKO08_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBD6320227.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYI"
     gene            complement(109..399)
                     /locus_tag="KKO08_004239"
     CDS             complement(109..399)
                     /locus_tag="KKO08_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBD6320228.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(399..>630)
                     /locus_tag="KKO08_004240"
     CDS             complement(399..>630)
                     /locus_tag="KKO08_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBD6320229.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          149 a          181 c          136 g          164 t
ORIGIN      
        1 caatatacga ccagcgcccc cacatacgta gttttccctg aatccagaca ctttccagcg
       61 tggtgagacg aaggtgttct ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc
      121 ctcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc
      181 ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc acgagctaca
      241 ggcaatcgct gcctgcatat cgtgtggctt tatcgctgtt ccgcacgttc ccgccagtcg
      301 gtaatgcgcc agcacagacg tttccggatc gtgattgcag tagccaggaa ttctgacggt
      361 gcacatctgc ccccgcgccg ctttaagtaa atccaccatt acgcaaactc cagcagctgc
      421 gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacagaat ccagttccac
      481 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat attcgcaaac
      541 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
      601 ccggcctgaa tggctaccca ctcgcggaaa
//
LOCUS       DAECNO010000285          629 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.294, whole genome shotgun sequence.
ACCESSION   DAECNO010000285 DAECNO010000000
VERSION     DAECNO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.294"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>629
                     /locus_tag="KKO08_004241"
     CDS             <1..>629
                     /locus_tag="KKO08_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBD6320230.1"
                     /translation="TLADNFKQKNEALDTWYVGMNDFARIAGGQNSRSNILSPRAFLE
                     FLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGERADLS
                     KVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDDSSLHLITNKVLAC
                     YQREANKKIARLLNNHQKLNNSQELNNSQVSCKGSVDSTITDLLEKALN"
BASE COUNT          216 a           94 c          137 g          182 t
ORIGIN      
        1 acattagccg acaattttaa acaaaaaaat gaagccttag atacctggta tgtgggaatg
       61 aatgattttg cccgaattgc cggggggcag aatagcagaa gcaatattct ttctcctaga
      121 gcatttttgg agtttttggc aaagatattt accctgggtt atgtggattt tagcaaacgc
      181 tccaacgaag cgggtagaaa tatgatggct catattgagt tctcatctta ttctaaagat
      241 actgatggca atgaaaaaat gaagttttac atgaataatc ctgaagggga gcgagcggat
      301 ttatctaagg tgaagataga aattacactt gcctcagctt ctactaaagg gattcgtgaa
      361 ggacatacag tcattatatt taaacaatct gatggttcga ctaatcgtta tgaagggaag
      421 tcttttgaaa gaaaagatga tagttcatta cacctgatta ctaacaaggt tctggcgtgt
      481 taccaacgcg aagctaacaa gaaaatagcg cgtctattaa ataatcatca gaagttaaat
      541 aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga ttctacgatt
      601 acggatttat tagaaaaagc attgaataa
//
LOCUS       DAECNO010000286          628 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.295, whole genome shotgun sequence.
ACCESSION   DAECNO010000286 DAECNO010000000
VERSION     DAECNO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.295"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KKO08_004242"
     CDS             complement(<1..131)
                     /gene="csgC"
                     /locus_tag="KKO08_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBD6320231.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQI"
     gene            complement(190..555)
                     /gene="csgA"
                     /locus_tag="KKO08_004243"
     CDS             complement(190..555)
                     /gene="csgA"
                     /locus_tag="KKO08_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBD6320232.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT          164 a          142 c          150 g          172 t
ORIGIN      
        1 aatatttgta ctctgcacag acaagattga gtaagagtaa cttcaggaat aatggtgtac
       61 atatcccctt gctgggtcgt attaaaggtt atctggctgg aaagtgccgc aaggagtaat
      121 aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca
      181 aatgatgtat taatactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
      241 gacggaggag ttcgatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
      301 aacagtcatt tcagaatttt tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc
      361 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
      421 accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc
      481 agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc tattattact
      541 gaggtgtact ggcaatagcg gacactacca tttgttcttt tttaagcagc catctgatga
      601 tatttttccc tgaaggctgc cggggaga
//
LOCUS       DAECNO010000287          621 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.296, whole genome shotgun sequence.
ACCESSION   DAECNO010000287 DAECNO010000000
VERSION     DAECNO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.296"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            78..621
                     /locus_tag="KKO08_004244"
                     /pseudo
     CDS             78..621
                     /locus_tag="KKO08_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          144 a          158 c          165 g          154 t
ORIGIN      
        1 gatggggcgc ttacaaacga atcggaaaat cgatactgga aaaactctaa ctcggtggcc
       61 agtttttgtt gaccacttca ggtcaggaga ttgtcgtcga accgattgaa caggctattc
      121 tcatctctga gcagactaag ctgtgggacg ccaagtcatc caccggattc actcagggca
      181 cacttcagca ggacgctatg aacatctgtg agaatgtggt ccgtaccatc aacgctcgca
      241 tggtcgacgt tctggaatcc tctaagctgc tgaagaccgt agagcttcct gtcctgactg
      301 gtagcctgac cgctaaggca gacgctatca tggacgctct gtatgagaac accgagcctt
      361 ctttcggttc tgaggtgtct gactacggta ttatcgccca tgagagccaa ctgaaagcac
      421 tgtcccgtct ggctgctaaa cagggcttct ctggtgaaga cgctatcgtg gatatgctgg
      481 gtaccgacat cgcgtattac aacggtgagg acaaaggtgt gttcatgttg gctaaacgct
      541 tcacggccct gagcttcggc tgcttccgtc acgacggtga gaatattacg gtcgtccttt
      601 cacgtgatgg cgactctcag t
//
LOCUS       DAECNO010000288          601 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.297, whole genome shotgun sequence.
ACCESSION   DAECNO010000288 DAECNO010000000
VERSION     DAECNO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.297"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..127
                     /locus_tag="KKO08_004245"
     CDS             <1..127
                     /locus_tag="KKO08_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBD6320233.1"
                     /translation="VTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(161..364)
                     /locus_tag="KKO08_004246"
                     /pseudo
     CDS             complement(161..364)
                     /locus_tag="KKO08_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            464..>601
                     /locus_tag="KKO08_004247"
     CDS             464..>601
                     /locus_tag="KKO08_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBD6320234.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          156 a          146 c          137 g          162 t
ORIGIN      
        1 ggtcacctgc aaacagaacg aagtggagcc ggaggactgg ttgcgcgaag tgatcgagaa
       61 gctcaatgac tggccgtcga accaagtgca tgaactgctg ccctggaact tctcgtctgt
      121 aaaataatcc ttacgctacg tacttctcgg ggcgcttacc tggagaataa cgtttatcca
      181 tattcttcca gtctggttca tcttccgacg tcatcaccgg gttgaaatta cgccgatata
      241 aatcgagttc cgtcacctgt attttccgct ccattgcccg ctgatggatc gcatcagcaa
      301 tatttgcggt caatgaatca tgacgaggat gggcccatac caaataaata cgttcagact
      361 gcataatttt acctccacgt taagttctgg agcgcacttt acgcaggcgg cataatcctg
      421 taaacgtgat gattcaggat gagttaatga aggattttca ctaatgcgga tgaatatgtc
      481 tgactttgcc acttcctttg ccgtggcccg taatcaaagc tttcgtgcag caggcgatga
      541 gttaggctta tcctcgtccg ccattagcca tagtattaaa acactggaac aacgtcttaa
      601 a
//
LOCUS       DAECNO010000289          601 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.298, whole genome shotgun sequence.
ACCESSION   DAECNO010000289 DAECNO010000000
VERSION     DAECNO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.298"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            245..320
                     /locus_tag="KKO08_004248"
     tRNA            245..320
                     /locus_tag="KKO08_004248"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Ile,seq:cat)
BASE COUNT          169 a          109 c          144 g          179 t
ORIGIN      
        1 tatattatta gatacaaaat cattacaaag tatcattgaa tcgatgataa ctatagatag
       61 tgctaattat taacatttgc ttgttgactg cgagtatcga ctttgcgaat ggaggcggtg
      121 tcgggtttta tgtcgcgtgt aatcacgacc caaggtgaat gggaacgcat aaaaatctgt
      181 tatttacatc ataagatcat catgtttatt gcgtggttta taagtattgt tattgcatac
      241 ttaaggccct ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc
      301 aagtccagca agggccacca aatcatcatt catgtatcag gaagagttaa gggtacaagg
      361 ggtactcgat ggcggtctat atttcatgac taacgccatt tcaaggaaga agtgcggtgg
      421 gtggtttcga atgagggctg gtgttttggt acgtggcacg ccactgcaat gtatttattc
      481 atcagtactc attcaaaagt acctggaaaa aatgcccgct atgccaagcg ggcaaattga
      541 tcctacccac gtaatatgga cacaggccta agcgaggttc tggttttcaa attgttccgg
      601 a
//
LOCUS       DAECNO010000290          580 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.299, whole genome shotgun sequence.
ACCESSION   DAECNO010000290 DAECNO010000000
VERSION     DAECNO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.299"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..580
                     /locus_tag="KKO08_004249"
                     /pseudo
     CDS             1..580
                     /locus_tag="KKO08_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a          149 c          160 g          129 t
ORIGIN      
        1 aacgggttac cgacgaacgg gacatattaa aaaaagccgc ggcgtacttc gcaaagctgt
       61 ccgactgagg tacgccttta tccgtgacaa ctcctgttgc tggcctgttc gcctgctctg
      121 tcgggtgctg gatgttcatc ccagtggttt ttacgcctgg cttcagcagc cgcattcaca
      181 acgccatcag gcagacctga gactgacagg acagattaaa cagttctggc tggaatcggg
      241 atgcgtctat ggttatcgca aaatccatct ggatctgcgt gacagcgggc aacagtgcgg
      301 agtaaacaga gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg
      361 aagcccgcgg gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca
      421 gttcaatccg gatgctcctg atgagcgttg ggtaacggac ataacctaca tcaggaccca
      481 cgaaggctgg ctgtatcttg ccgttgttgt tgatctgttc tcacgcaaaa ttatcggctg
      541 gtccatgcaa tcccggatga caaaggacat tgtcctgaac
//
LOCUS       DAECNO010000291          578 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.300, whole genome shotgun sequence.
ACCESSION   DAECNO010000291 DAECNO010000000
VERSION     DAECNO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.300"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>578
                     /locus_tag="KKO08_004250"
     CDS             <1..>578
                     /locus_tag="KKO08_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HBD6320235.1"
                     /translation="ARSIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGH
                     EHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSP
                     DSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWD
                     NSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT          138 a          143 c          185 g          112 t
ORIGIN      
        1 gcaagaagca tcgccacaat ggcaacccgg agaggctacc agatgggacg ctggcttgct
       61 ggcaggctca tgaaagagct ggggctggtc agctgtcagc agccgactca ccggtataaa
      121 cgtggtggtc atgaacatgt tgctatccct aactaccttg aaaggcagtt cgccgtgacc
      181 gagccaaatc aggtgtggtg cggtgatgtg acctatatct ggacgggtaa gcgctgggcg
      241 tacctcgccg ttgttctcga cctgttcgca agaaaaccag tgggctgggc catgtcgttc
      301 tcgccggaca gcaggctcac catgaaagcg ctggaaatgg catgggaaac ccgtggtaag
      361 cccggcgggg tgatgttcca cagcgatcag ggcagtcatt atacgagcag gcagttccgg
      421 cagttattgt ggcgatacca gatcagacag agtatgagcc ggcgcggaaa ctgctgggat
      481 aacagcccaa tggaacgctt cttcaggagt ctgaagaacg aatggatgcc ggtggtgggt
      541 tacgtaagct tcagcgaggc agctcacgcc ataacgga
//
LOCUS       DAECNO010000292          566 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.301, whole genome shotgun sequence.
ACCESSION   DAECNO010000292 DAECNO010000000
VERSION     DAECNO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.301"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..297
                     /locus_tag="KKO08_004251"
     CDS             <1..297
                     /locus_tag="KKO08_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBD6320236.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="KKO08_004252"
     CDS             complement(405..>566)
                     /locus_tag="KKO08_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320237.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAECNO010000293          530 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.302, whole genome shotgun sequence.
ACCESSION   DAECNO010000293 DAECNO010000000
VERSION     DAECNO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.302"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
BASE COUNT          172 a           78 c          107 g          173 t
ORIGIN      
        1 tgaatatttc agagttggag ttcgaatgtt gacacctgag tatttcctgc taaaaatagc
       61 cctgctttac ggatggttgt attgcaataa cacgaaagta gtaagagatt aaaggtatct
      121 gtcagcgata actctgcaag tgatgacatt ttgtaaacca aataatgtaa atcagcaatg
      181 tatcttttca cttaattata acaaatgtgg tattcactaa gaatctgata cgagcttaca
      241 tcacgactat tttagtctca tcgcgggata cagggatgca ccgatggatc tcagcacacc
      301 gtaatgcaat acctgaacaa atagaacagt aaagtggttc agtattaatg aaagctctta
      361 ttttccgttg ggaaaactta aaatagatga gaaggctttt tgatttctta tgtgatctca
      421 tttcgagtat tgagaaattt gtgattttta aatttttcaa taagccctgt aagggattgg
      481 ggttttatga gaaataaaga gacgtattgt ctttgtttaa acggtacgta
//
LOCUS       DAECNO010000294          529 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.303, whole genome shotgun sequence.
ACCESSION   DAECNO010000294 DAECNO010000000
VERSION     DAECNO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.303"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..238
                     /locus_tag="KKO08_004253"
                     /pseudo
     CDS             1..238
                     /locus_tag="KKO08_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            338..>529
                     /locus_tag="KKO08_004254"
     CDS             338..>529
                     /locus_tag="KKO08_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320238.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDL"
BASE COUNT          181 a          109 c          104 g          135 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcatat tggctacgta attaaaacag
       61 aaaataaaga aaccccattc actgtagttg atatcgacgg tctatcagga aactttaaaa
      121 ctcttgttga aggtgccaca aaaatgtgcc tagtttacat cggaaataat ctgcccgcag
      181 aaaaaaagcc gaatttctgg caaatctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
      241 aaaagaaagc ctgcacagtg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      301 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg agcacaaaac cactcttcct
      361 gttacggaaa gcgaaaaaat catccggtga acctgacgtc gtcctgtggg caagcgacga
      421 ttttgaatcg accagcacca ctctggacta tctgctcgtt aagtcaggta aaaaactgag
      481 caactatttt aaagctgttg ccacaaattt tcctgtcgtt aatgacctt
//
LOCUS       DAECNO010000295          510 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.304, whole genome shotgun sequence.
ACCESSION   DAECNO010000295 DAECNO010000000
VERSION     DAECNO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.304"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..>510)
                     /locus_tag="KKO08_004255"
     rRNA            complement(<1..>510)
                     /locus_tag="KKO08_004255"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           96 a          169 c          114 g          131 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcagaccag ctagggatcg
//
LOCUS       DAECNO010000296          509 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.305, whole genome shotgun sequence.
ACCESSION   DAECNO010000296 DAECNO010000000
VERSION     DAECNO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.305"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>509
                     /locus_tag="KKO08_004256"
     rRNA            <1..>509
                     /locus_tag="KKO08_004256"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          131 a          124 c          149 g          105 t
ORIGIN      
        1 acggccgcaa ggttaaaact caaatgaatt gacgggggcc cgcacaagcg gtggagcatg
       61 tggtttaatt cgatgcaacg cgaagaacct tacctggtct tgacatccac ggaagttttc
      121 agagatgaga atgtgccttc gggaaccgtg agacaggtgc tgcatggctg tcgtcagctc
      181 gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa cccttatcct ttgttgccag
      241 cggtccggcc gggaactcaa aggagactgc cagtgataaa ctggaggaag gtggggatga
      301 cgtcaagtca tcatggccct tacgaccagg gctacacacg tgctacaatg gcgcatacaa
      361 agagaagcga cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt ccggattgga
      421 gtctgcaact cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg
      481 tgaatacgtt cccgggcctt gtacacacc
//
LOCUS       DAECNO010000297          483 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.306, whole genome shotgun sequence.
ACCESSION   DAECNO010000297 DAECNO010000000
VERSION     DAECNO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.306"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(52..483)
                     /locus_tag="KKO08_004257"
                     /pseudo
     CDS             complement(52..483)
                     /locus_tag="KKO08_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
BASE COUNT          100 a          122 c          126 g          135 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc cgatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg tgggcgtctg cctgcgcgtt ttcttcctgg cctcccgcca gttctttggt
      361 ttcttgagca ttatcctggt ggtgaacgtc gtctggcgct gctcccggtt ttagttccca
      421 tgtcatggag tctttgctga gttgatagcg ttcactccag gtaaaatcga tctcaccttc
      481 agg
//
LOCUS       DAECNO010000298          455 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.307, whole genome shotgun sequence.
ACCESSION   DAECNO010000298 DAECNO010000000
VERSION     DAECNO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.307"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..>455
                     /locus_tag="KKO08_004258"
     CDS             <1..>455
                     /locus_tag="KKO08_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBD6320239.1"
                     /translation="IQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVF
                     WRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAE
                     QLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEEL"
BASE COUNT          115 a          133 c          128 g           79 t
ORIGIN      
        1 attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac gaaaacagcg
       61 cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct gctggccatc
      121 gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt cccccgccag
      181 acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca gatggaactg
      241 accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga aaatgagctt
      301 gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga taaaggttat
      361 tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca ctggatgata
      421 cctctcagaa agggagcgca atatgaagag ctcag
//
LOCUS       DAECNO010000299          446 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.308, whole genome shotgun sequence.
ACCESSION   DAECNO010000299 DAECNO010000000
VERSION     DAECNO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.308"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..395)
                     /locus_tag="KKO08_004259"
     CDS             complement(<1..395)
                     /locus_tag="KKO08_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320240.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          105 a          123 c          123 g           95 t
ORIGIN      
        1 gtaccgctta ttgcgtgacc gatgacgtca atcatccatg ctgacaggtt ttgatgagca
       61 agttgctcga gtgattcgga tgtctggtca cgcagctggt tgtcgcagtg ccagcacaat
      121 accattgcgc cggtaccata acggtgaatg acggtttcgc tgtgatgata atcgccgtgt
      181 ggccactggc aggatttaat atggcgcaac agccagtcag acaatgcgcc agcaccacca
      241 gcagcacgaa tcacccgtgc gttactgaaa aacggcagca atgttttgtc ttccactagc
      301 ggctggcgaa cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc
      361 tccaccagta cccgggtatt gtggaatacc ggcatggatt cacgaccggg cttaaggacc
      421 accagcccga gttccggaac cagaac
//
LOCUS       DAECNO010000300          445 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.309, whole genome shotgun sequence.
ACCESSION   DAECNO010000300 DAECNO010000000
VERSION     DAECNO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.309"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..445)
                     /locus_tag="KKO08_004260"
     CDS             complement(<1..445)
                     /locus_tag="KKO08_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HBD6320241.1"
                     /translation="MLSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          101 a          113 c           86 g          145 t
ORIGIN      
        1 catgttcgaa caccactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
       61 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
      121 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
      181 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
      241 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
      301 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
      361 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
      421 atgatgctac ccggttgctt agaat
//
LOCUS       DAECNO010000301          444 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.310, whole genome shotgun sequence.
ACCESSION   DAECNO010000301 DAECNO010000000
VERSION     DAECNO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.310"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(<1..373)
                     /locus_tag="KKO08_004261"
     CDS             complement(<1..373)
                     /locus_tag="KKO08_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBD6320242.1"
                     /translation="MVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFGGM
                     VRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEFDI
                     WNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           95 g          109 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcg
//
LOCUS       DAECNO010000302          434 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.311, whole genome shotgun sequence.
ACCESSION   DAECNO010000302 DAECNO010000000
VERSION     DAECNO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.311"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            35..434
                     /locus_tag="KKO08_004262"
                     /pseudo
     CDS             35..434
                     /locus_tag="KKO08_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAECNO010000303          418 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.denovo.312, whole genome shotgun sequence.
ACCESSION   DAECNO010000303 DAECNO010000000
VERSION     DAECNO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.denovo.312"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            1..418
                     /locus_tag="KKO08_004263"
                     /pseudo
     CDS             1..418
                     /locus_tag="KKO08_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          102 a          116 c          123 g           77 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaa
//
LOCUS       DAECNO010000304         7544 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.guided.152, whole genome shotgun sequence.
ACCESSION   DAECNO010000304 DAECNO010000000
VERSION     DAECNO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.guided.152"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..304
                     /locus_tag="KKO08_004264"
     CDS             <1..304
                     /locus_tag="KKO08_004264"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBD6320243.1"
                     /translation="EKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVAS
                     ALLKPFIKSESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            550..1593
                     /locus_tag="KKO08_004265"
     CDS             550..1593
                     /locus_tag="KKO08_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBD6320244.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            1655..2269
                     /locus_tag="KKO08_004266"
                     /pseudo
     CDS             1655..2269
                     /locus_tag="KKO08_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2326..3783)
                     /gene="pepD"
                     /locus_tag="KKO08_004267"
     CDS             complement(2326..3783)
                     /gene="pepD"
                     /locus_tag="KKO08_004267"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBD6320245.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4045..4503
                     /gene="gpt"
                     /locus_tag="KKO08_004268"
     CDS             4045..4503
                     /gene="gpt"
                     /locus_tag="KKO08_004268"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBD6320246.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4595..5839
                     /gene="frsA"
                     /locus_tag="KKO08_004269"
     CDS             4595..5839
                     /gene="frsA"
                     /locus_tag="KKO08_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBD6320247.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5897..6298
                     /gene="crl"
                     /locus_tag="KKO08_004270"
     CDS             5897..6298
                     /gene="crl"
                     /locus_tag="KKO08_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBD6320248.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(6337..7392)
                     /gene="phoE"
                     /locus_tag="KKO08_004271"
     CDS             complement(6337..7392)
                     /gene="phoE"
                     /locus_tag="KKO08_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBD6320249.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GIIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1844 a         1840 c         1913 g         1947 t
ORIGIN      
        1 ggagaaactc gcgaaatcac aagtgcgggt tgcagttatt aatgcaaaac tggtcggctt
       61 tattacctgc gttgaacact atatcgatat gttatttgtt gaccctgaat acacccgccg
      121 tggggttgcc agcgctttgt taaaaccttt tattaagtct gaatccgaac ttacggtgga
      181 cgcaagcata accgcaaaac ccttttttga acgttatggt tttcagacag ttaagcagca
      241 gcgcgttgaa tgccggggag cgtggtttat taatttttat atgcgttata aaccgcaaca
      301 ttaaatccag cttgcaatga aaataacgcc cgcctagtat gtgcgcgttt tcttattcca
      361 caaactgcaa ggaggtaaat catgacaaat cctttatcaa tgactctttg cagacctttc
      421 caggattaat tctttttttc ttgccctgga ttcgtctgcc atttcctgat tttttaattg
      481 ttactctcaa taattcaagt cgcagcacat gcaacttgaa gtatgacgag tctatttacg
      541 gaatggtata tgggcaatta tattcgtccc ttatccgatg cggtatttac catcgcatct
      601 gatgacctgt ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc
      661 aacatgcagc gcgtagtggg gatgccagat ttacaccccg gacgcggcta cccgattggc
      721 gcagcgttct tctccgcagg ccgtttttac ccagcactgg tcggcaatga tatcggctgt
      781 ggcatggcgc tatggcaaac agatattctc gctcgcaaat acaacgccga taagtttgaa
      841 aagcgattat cagctttgga tgacgttgct gaagaaagct ggctggagga aaacctgccg
      901 tcagcatttg cacagcatcc gtggcgcagc tcgcttggct ctatcggtgg tggcaatcat
      961 ttcgctgaac tgcaacaagt tgatcaaatc atcaacgctg aactgtttgc actggcaggt
     1021 ctggatgcgc agcatctgca actgctggtt catagcggct cgagggggct agggcagtct
     1081 attttacagc ggcatattgc ctcgttttcg catcatggtt tgcctgaagg cagtgacgac
     1141 gcgctggcgt ttgcgcgttt taatcgccac ctgatcgctt tgcgcataat gcaacaggtt
     1201 aaggccaccg gaagtccggt tctggatgtg gcgcataact ttgttagcgc gtgtcgaatc
     1261 ggtgatcaac aaggggtctt acatcgtaaa ggtgccacgc ctgatgactg tggtctggtg
     1321 gttattcccg gttcacgcgg tgattactcc aggctggttc agcccgtcag gagtgaggaa
     1381 acattgcatt cgctggcgca tggggctggg cgtaaatggg ggcgcaccga gtgtaaaggg
     1441 cgtctggcag cgaaatacac agcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta
     1501 atttgtcgcg ataaacaact catcttcgaa gaagcgccac aagcctataa atcggctgaa
     1561 agtgtggtgc aatgtctggg caggccggat taattattcc tgtcgcgcgg ctgcgtccgg
     1621 tgctaacgct caaaaacagt ggagggaaaa aaggatgatc ttgctataac tctcctcagc
     1681 gcaggggccg gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga
     1741 agctgcccga caagacgtcg cggtaacggt gctggaaaca gaaacaggcc gctactctga
     1801 cacgctgcgt tcggcgctga tttctctgga tggcgacaac gcatgggcgt taagcgaaag
     1861 ctggtgtggc actattcagt ggatttgccc gagcccgtat cgcccacatc acgggcgtaa
     1921 aaactggttt ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat
     1981 ccggtatgag acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc
     2041 ggcggtacgc gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag
     2101 ccagcatgct aacaaacggc tggcgcgatt gctgattgcc tggaagctgg atcagcagca
     2161 ataggaaaat agcgcggtgc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg
     2221 tggcaacccg cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa
     2281 actgccggaa ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat
     2341 ttctttcagc agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg
     2401 ctcatccgga gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg
     2461 atacggtttt ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt
     2521 cttgttgaac aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc
     2581 cggctgccag ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag
     2641 cgaatccagc atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca
     2701 gtgaatttct acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac
     2761 cacacctttt gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg
     2821 aataaaggta tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag
     2881 caacaaggcc agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt
     2941 attcaccaga gatttcagtg cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc
     3001 ttcacgcggg atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag
     3061 ttcttctgca tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac
     3121 gtggatttca ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc
     3181 aaaaccagct ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgatcccccc
     3241 cgcacaaccc atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc
     3301 ctgcaaccag ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt
     3361 cagcagcact tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga
     3421 ggccatacca atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc
     3481 atcaatataa ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg
     3541 cggcaccata tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt
     3601 agcaggttta cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc
     3661 ttttgcccaa ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat
     3721 agaacagatt ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga
     3781 cacgttaagt ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag
     3841 aaaatgttgt caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc
     3901 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3961 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     4021 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     4081 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     4141 attgccgtaa gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt
     4201 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     4261 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     4321 accggtggta ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     4381 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     4441 acctggatcg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     4501 taatcttttc aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca
     4561 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     4621 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     4681 cggcgcacaa ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg
     4741 catgattaac cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt
     4801 ccaggcacgt attgtgatga gcgatgctga acgtaccgac gatgatttat acgataccgt
     4861 gattggctac cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca
     4921 acaaaaagcc tgtacggaag aagatccgaa actcagtggt cgacactggc tgcatgcggc
     4981 tacgttgtac aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc
     5041 gcaggcttta tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg
     5101 gcagatggag tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa
     5161 aggcgatggc ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga
     5221 ctattacagc ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga
     5281 tatgccgtcg gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca
     5341 tcagcacgtc ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc
     5401 ctttggtttc cgtttcggcg ctaacgttgc cgtgcgtctg gcataccttg aatcgccgcg
     5461 tctgaaagcg gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg
     5521 ccagcaacag gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga
     5581 tgcttccgac gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt
     5641 gctggggcgt cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag
     5701 cccggaagag gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat
     5761 cccatttaac ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat
     5821 cgaaaaacgc ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa cagttgcatc
     5881 acaacaggag atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa
     5941 atttaccgca ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt
     6001 cgattgtctg gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg
     6061 ctggtggatg gagctggaag cgcaggaatc ccgttttacc tacagctacc agtttggtct
     6121 gttcgataaa gcaggcgact ggaagagtgt tccggtaaaa gatactgaag tggttgaacg
     6181 actggagcac accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa
     6241 tctgaagctg gaaccggcga atgattttcg tgatgaaccg gtgaagttaa cggcgtgagt
     6301 gaaatgtgcc ggatgcatca catccggcaa tattcattaa aactgatacg tcatcccaac
     6361 cgcgacagta tcatcattat taatattcag cttattatcg ctatccagtt ggttgatttt
     6421 ataatcaaca aacgctgaca tatttttgtt gaaataatac gtagcaccga cgtcgatata
     6481 attgaccaga tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc
     6541 cagcgatgga cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgtgt
     6601 cttattggca aagccgccag ttattggcgt cattttgcgt gtttcagaat agaaagttgc
     6661 cagataaata ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc
     6721 tgtgccacgg ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat aggccccact
     6781 aatggcgaaa tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc catcgccgtt
     6841 ttgcttttta acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc
     6901 atcgataatg ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt
     6961 cataaagttg tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca tatcggtcca
     7021 ggcttccacg tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt
     7081 atatttcaac ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt
     7141 atttccggca aactccgctt cccaccgacc ataaccagtc agttgatcgt taatttgagt
     7201 ttcaccttta aaaccaaaac ggatataact ctggtcgcca tctttactgg cgttatcact
     7261 catataatgc atggctttaa ctttgccata gacatccagt ttattaccgt ctttattata
     7321 tatttctgca gcctgtacag atgcagatgc cacaatgccc atcaccacta atgccagagt
     7381 gctctttttc attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc
     7441 cgttgaaaac aggaaagttt ttaacctgag attgttaaag atatattaca gattaatgat
     7501 attctgaaaa tgggtaatgc tgccaactta ctgatttagt gtat
//
LOCUS       DAECNO010000305         7172 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.guided.154, whole genome shotgun sequence.
ACCESSION   DAECNO010000305 DAECNO010000000
VERSION     DAECNO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.guided.154"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..984
                     /locus_tag="KKO08_004272"
     CDS             <1..984
                     /locus_tag="KKO08_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBD6320250.1"
                     /translation="GEPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRH
                     LGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFA
                     VGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQ
                     RLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRIT
                     DTSSVFNTDLLYTIELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKH
                     TLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            977..1711
                     /gene="frdB"
                     /locus_tag="KKO08_004273"
     CDS             977..1711
                     /gene="frdB"
                     /locus_tag="KKO08_004273"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBD6320251.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1722..2117
                     /gene="frdC"
                     /locus_tag="KKO08_004274"
     CDS             1722..2117
                     /gene="frdC"
                     /locus_tag="KKO08_004274"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBD6320252.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2128..2487
                     /gene="frdD"
                     /locus_tag="KKO08_004275"
     CDS             2128..2487
                     /gene="frdD"
                     /locus_tag="KKO08_004275"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBD6320253.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2550..3683
                     /locus_tag="KKO08_004276"
     CDS             2550..3683
                     /locus_tag="KKO08_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBD6320254.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            3772..4305
                     /gene="blc"
                     /locus_tag="KKO08_004277"
     CDS             3772..4305
                     /gene="blc"
                     /locus_tag="KKO08_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBD6320255.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4302..4619)
                     /gene="sugE"
                     /locus_tag="KKO08_004278"
     CDS             complement(4302..4619)
                     /gene="sugE"
                     /locus_tag="KKO08_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBD6320256.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(4795..4941)
                     /gene="ecnB"
                     /locus_tag="KKO08_004279"
     CDS             complement(4795..4941)
                     /gene="ecnB"
                     /locus_tag="KKO08_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBD6320257.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(5052..5177)
                     /gene="ecnA"
                     /locus_tag="KKO08_004280"
     CDS             complement(5052..5177)
                     /gene="ecnA"
                     /locus_tag="KKO08_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBD6320258.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(5229..5795)
                     /gene="efp"
                     /locus_tag="KKO08_004281"
     CDS             complement(5229..5795)
                     /gene="efp"
                     /locus_tag="KKO08_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBD6320259.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            5837..6865
                     /gene="epmB"
                     /locus_tag="KKO08_004282"
     CDS             5837..6865
                     /gene="epmB"
                     /locus_tag="KKO08_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBD6320260.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1801 a         1842 c         1851 g         1678 t
ORIGIN      
        1 ggcgagccga aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc
       61 tggcacgaat ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc
      121 gacctgcgtc acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg
      181 gcgaaagcgt acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca
      241 cactacacca tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg
      301 ttcgccgtgg gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac
      361 tccctggcgg aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca
      421 gcaactgccg gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa
      481 cgtctgaaag atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa
      541 atgggcctgg caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa
      601 accatcgaca agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc
      661 tccagcgtgt tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt
      721 gctgaatgta tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt
      781 ctggacgaag gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc
      841 cgcgatgctg atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg
      901 ccagctaaac gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat
      961 aataaggaga aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa
     1021 cccggaagtc gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac
     1081 ctcattactg gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg
     1141 ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa
     1201 actggcatgt aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc
     1261 taacttcccg attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga
     1321 agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca
     1381 gaccccggcg cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg
     1441 ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac
     1501 gctggcgcat cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca
     1561 gttgaacagc cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg
     1621 cccgaaacac gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga
     1681 ctttcttatc gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac
     1741 cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca
     1801 tgctgcgcga aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc
     1861 tgtttgccct gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc
     1921 cggttatcgt gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct
     1981 ggtttgaact ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag
     2041 agccaattat caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg
     2101 ttgccctgta ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa
     2161 ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg
     2221 atgatcctgc tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc
     2281 tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg
     2341 atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa
     2401 atccacgtgc ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc
     2461 acgctgattg gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg
     2521 cgggccgttt tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa
     2581 ttaccgcctc ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca
     2641 caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc
     2701 agggtaaacc ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca
     2761 cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg
     2821 gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg
     2881 aacttaccgc taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg
     2941 gcggcctgcc attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct
     3001 atcaaaactg gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta
     3061 tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc
     3121 aaactcgtgt cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag
     3181 aagaaaagaa ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg
     3241 cgttagatgc tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc
     3301 aaagcaattt accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg
     3361 cacaatctcg ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg
     3421 actggccggt caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag
     3481 cacgcccggt aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata
     3541 aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg
     3601 gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc
     3661 agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg
     3721 cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg
     3781 ctccctctcg ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg
     3841 ccgccgcgtg gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg
     3901 tatgagattg cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca
     3961 tacagcctgc gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga
     4021 ggaatgtggc agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg
     4081 ctgaaagtgt cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg
     4141 gaataccgcc atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc
     4201 acgccaacca tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg
     4261 tttgatgtca gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc
     4321 agaccaataa tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac
     4381 tcaccgagca gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg
     4441 gcataagccg tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact
     4501 atcatcgccg tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc
     4561 aggccaacgg cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata
     4621 tcaggctccg gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc
     4681 aaagctgatg gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt
     4741 atgacgatcg cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg
     4801 ctgcgctttc gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac
     4861 gccacgcgtg gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc
     4921 tgcaattgtc ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta
     4981 tagcaaatta ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa
     5041 gagaagaaaa attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg
     5101 ccgaaaccgc aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg
     5161 ataaggcgtt tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca
     5221 caaccgcatt acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact
     5281 tcgccgattt gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt
     5341 ttgccgccag tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt
     5401 tcaacgaagt tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac
     5461 tctgcctgat ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc
     5521 tgctcgaaag tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc
     5581 aggttcatat cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct
     5641 acgcgagtac cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc
     5701 ggttttacga attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga
     5761 ccagcacgaa aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg
     5821 gtagctaagc cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg
     5881 gttaacgcaa cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat
     5941 agacgcggac gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt
     6001 gccccgctca tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca
     6061 ggtacttacc tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga
     6121 agaacagcac agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct
     6181 ggtcaaaggc ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc
     6241 cgaaaatcag ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc
     6301 ggaactggac gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct
     6361 ggactggttg ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag
     6421 ccgtctgccg attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg
     6481 ttctacgctg caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac
     6541 gttccgtcag gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt
     6601 tctgttacgt ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt
     6661 cgatgccggc gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca
     6721 ttttatggtg agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc
     6781 gggatatctg gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct
     6841 ggatctccag ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc
     6901 tgatgcgcta cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa
     6961 ctgtaggccg gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc
     7021 aacctgtcat taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat
     7081 atgcattgct gttgttactt tttatatagc taacaccaat aattaacctt tcttttcggt
     7141 aatgactcca acttactgat agtgttttat gt
//
LOCUS       DAECNO010000306         5751 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.guided.172, whole genome shotgun sequence.
ACCESSION   DAECNO010000306 DAECNO010000000
VERSION     DAECNO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.guided.172"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(45..1060)
                     /locus_tag="KKO08_004283"
                     /pseudo
     CDS             complement(45..1060)
                     /locus_tag="KKO08_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1455..2111
                     /locus_tag="KKO08_004284"
     CDS             1455..2111
                     /locus_tag="KKO08_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="HBD6320261.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFV
                     NCKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRT
                     NFAHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLS
                     RGVFSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD
                     "
     gene            complement(2430..2846)
                     /locus_tag="KKO08_004285"
     CDS             complement(2430..2846)
                     /locus_tag="KKO08_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD6320262.1"
                     /translation="MDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTTTAAGKL
                     VFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMGQSETK
                     VSTLCQELGITRQTLYRHISPVGQLRADGIKLLNRG"
     gene            complement(2891..4282)
                     /locus_tag="KKO08_004286"
     CDS             complement(2891..4282)
                     /locus_tag="KKO08_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="HBD6320263.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLL
                     LNTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCR
                     CEESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNAST
                     VRNHLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRN
                     FEIIAGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADN
                     LRDLQFGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLW
                     HGNVVAALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRY
                     GEPVSTAFVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYP
                     GFQSDGKGPAMAA"
     gene            complement(4319..4891)
                     /locus_tag="KKO08_004287"
     CDS             complement(4319..4891)
                     /locus_tag="KKO08_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD6320264.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNR
                     PGLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGT
                     NNPMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALR
                     SRGVRLRIACDHAGLGVSTYYKLRHQMNNQ"
     gene            complement(5028..5618)
                     /locus_tag="KKO08_004288"
     CDS             complement(5028..5618)
                     /locus_tag="KKO08_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HBD6320265.1"
                     /translation="MKIYVIKIAVHGVSPMVWRRLRIAADTSLAALHFIFQIVQGWGD
                     DHLHQFHIYGKDYGISYEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIR
                     VEAIYENSTLKAPFCISGHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADD
                     LDAVRFNRHKINRQLSRLDLASPVLEPEVIWLGRRR"
BASE COUNT         1365 a         1518 c         1372 g         1496 t
ORIGIN      
        1 aacgtgagtt ttcgttccac tgagcgtcag acccctaaaa tcctgatgct gatgtagtcc
       61 cgttttatcg tcatcacgaa gctttctgct atgccattgc tttccgggct tcgcactgct
      121 gtcgtacaag gctccagtcc cagcaaccgg gcgaacgccg gcactgttgg caactgcctt
      181 ccgaagcgtt tttccaccgc acccagcatg acatcctgca ccgtttcttt gtcatagcct
      241 ccggtgctcg ctgcccagtc aatgatctcc ctgtcgcagc agtcctgcgc gaaggtcacc
      301 cgcagctttt cgccattatc acagcggaac tcaaagccat ctgagcacca gcgccggtta
      361 ctttccgcta ctgctacgcg ccccttatgg gcccgcttac ggcaaggatc agcgggttta
      421 cgttcaagaa gcagattatg cgttctcata atgcgataca cccgctttgc attaaccaca
      481 ggcaggccgt cccgctccga ctcccgtcgc agcagcgccc acacacggcg ataaccatac
      541 gtcggcaggt cagccaccgc catgtttatc cgggacaata cggtggtatc gtcagaacgg
      601 ggctgccgtc tgcgatcctg tcagtcagat ggccggtgaa cccgaatgct caactgcgca
      661 cgcgacacgc caagacttct gcaaacgtcg cttattcgtc gttcccaggc aacgagggcg
      721 catgcgcaat ccattttttt gcccggccga actccacggc ctctttgagt atctcggctt
      781 ccatcgtctt tttgcccaga aggcgctgga gttcgcgaat ttgcttattg gcggcagcga
      841 gttcggaggc cggcaccact tcctcgcccg atgcaacagc cgtcagtgaa ccgtcttaat
      901 attgcttgcg ccatttgaat atctggtttg cattgatgcc atgcaggcgc gcgacatgag
      961 acacggtcat accgggctcc atagtctgct gaataatggc aattttttcc tgcggtgtac
     1021 gacgccgacg ccgctcaggt cctgataaca cttcaaccat cttatcgttc tgactggtat
     1081 taaacatagt tccaagacta cctcttattt taagagagtc gaagtgtctg gtgatctatg
     1141 gggccagtct agccctcttc caagtgtagg gagatatagt caaagtttgc ttctccgccg
     1201 atgagtccgc cctgcgtcgg atagtccgat tttttaccgg cgaagcacgg ctctcctaac
     1261 cctatctaaa gcgaataact tttttcaaca gggccgcgtg ccaatgcaag agcgataggc
     1321 gctctacgcg ccaatttgac cacttaaaac aggtaaattg aggggttgta atgtgttgat
     1381 gtaacaggct tttattttaa tgtcttggca tatgtataat ggtagtctag ccctcctttc
     1441 aacaaggagt actcatggaa acctacaatc atacatatcg gcaccacaac ttttcacata
     1501 aagacttaag tgatctcacc ttcaccgctt gcacattcat tcgcagcgac tttcgacgtg
     1561 ctaacttgcg tgatacgaca ttcgtcaact gcaagttcat tgaacagggt gatatcgaag
     1621 gctgccactt tgatgtcgca gatcttcgtg atgcaagttt ccaacaatgc caacttgcga
     1681 tggcaaactt cagtaatgcc aattgctacg gtatagagtt ccgtgcgtgt gatttaaaag
     1741 gtgccaactt ttcccgaaca aactttgccc atcaagtgag taatcgtatg tacttttgct
     1801 cagcatttat ttctggatgt aatctttcct atgccaatat ggagagggtt tgtttagaaa
     1861 aatgtgagtt gtttgaaaat cgctggatag gaacgaacct agcgggtgca tcactgaaag
     1921 agtcagactt aagtcgaggt gttttttccg aagatgtctg ggggcaattt agcctacagg
     1981 gtgccaattt atgccacgcc gaactcgacg gtttagatcc ccgcaaagtc gatacatcag
     2041 gtatcaaaat tgcagcctgg cagcaagaac tgattctcga agcactgggt attgttgttt
     2101 atcctgacta attgctttga tgtgtgattt taaacgctca aatttataaa acgaataata
     2161 ttgtcaacct tgcaataata gacataggga caaccatgta taaattttta ccctcaggcg
     2221 tcttcacaat agagccatat agcagtgggt cacttagtgg gcttaatttt tccattaaag
     2281 ataatattaa tattgctcag tataaaacat catatggtag cccatcttgg caagtaaaca
     2341 taaggctgcc atttataagg cactgttgca aaaatgtgga ggtgataggg gtactacgcc
     2401 ggaaccccag atttttccgt ccgttcaaat tatccacggt tgagcagctt gatcccgtcc
     2461 gcccgcagtt gaccaacagg ggaaatatgc ctgtacaaag tttgccgggt tatcccaagt
     2521 tcctggcata gcgtactgac ctttgtttct gattgtccca ttgatgccat tgccagtcgc
     2581 agtttgactg gcgtcatttt ataaggtcgg ccgccgttcc gcccacgggc tcttgctgat
     2641 gccagacctg cagtcgttct ttcagcaatc agttcgcgct caaactccgc cagtgcggca
     2701 aagataccga agacaagctt gccagcggcc gttgttgtgt cgatagtcgc cccgtgaccg
     2761 gtcagcactt tcagaccggt ccccctcgcg gtcaggtcgt gcactgtatt aatgagatga
     2821 cgaagatcac gaccgaggcg gtccagcttc catacgaccg agagcataaa aaatattggg
     2881 gcagcgtgag ctatgctgcc atcgctggtc ccttcccgtc actttgaaaa cccggatacc
     2941 agcagacaaa tttatcctgc agttcgttat tcaaaacggc ggtccgggtc tgtaacaaat
     3001 aatgagcccc ttttctgctc cactgcatct gctgcttttt ggccattcgt ctggcgatca
     3061 cttcattgat cgtggattcc acaaacgcgg ttgataccgg ctctccgtac cgacgcattt
     3121 cgccgtagtt tgggatcatc atcttattat tccggatgta ggtatacatt tcatccagat
     3181 gtttttgcag ggatttcagg ctaggatagc tgagttcagg gtcatcgcaa tacataacgc
     3241 aattatcaat atgttccagc gcagcgacaa cattgccatg ccagagatat cgtttaatgc
     3301 tttccagcag tgccagaact ttactccccg cctctggatc tgataccagc agtccccggg
     3361 catattgcat gagcaccttc agcctcatgg tgatatgaaa ccagtccagc acatgcgttg
     3421 actcagggta cataccgaac tggaggtccc tgagattatc cgcgccgtcg gacagaaaaa
     3481 atatctgctg gttggcctgc attccctgcg ctgaaaggtg ggtcatgagc ctgcgttccg
     3541 gatgacagtc atccttctga acaaaaccga agcggcgggt gtcagcaggc gcaccaacgg
     3601 aaaaagactt cccggcaata atttcaaaat tacgcttttt gtcatcccga tcgcgaacat
     3661 aaccaccatc tatacccacc acaagcggtt ttcctggcct gggcaggttc ccccagtcgc
     3721 gggggcagcc ggaaagaaaa cctgaatgag cttcagcctc agcgtcaaga cgctgcgcca
     3781 cctggcataa atgattcctc accgttgagg cattcaggct gtggcccacc ggcagaatat
     3841 ctttcagcag acgagtcgtc atttcatagg agatcatcga ggcccagcgg gtttcaatat
     3901 atttcagtgc cgggtgagaa taatcgccgg cccagtcgct gagcaaactg actgtctttg
     3961 tatcactctc ttcacaacgg caccggtaca cccgaagccc ggataccgga ataacgccaa
     4021 acagcgtccg gtaccgtatt ttctgtttgc ctttgattct gcgcgcagca aggcagtgag
     4081 ggcaccggat atggtgttga gtatattcgt ctgcctgcag ctggaccacc gactgctgga
     4141 ccgtattcag caacagcttg gattctgaca ccgataatcc gatgtcgttc cgggtctccc
     4201 ctgatttctg gattgtcatc agttcctccg ttcgactgga gcctgattca tcggtgatga
     4261 ctatctggag cgtcagttgc atcattgcct cctgctgtgg tggatgacta tctcccggct
     4321 actggttgtt catctggtga cgaagcttat aatatgtgga aacgccaagg cccgcatggt
     4381 cgcaggcgat cctgagcctg acccctctgc ttctgagtgc gtcaatttcc tggatctttt
     4441 tgatatcggc gccggggcgc cctgtttttt caccccgtgc ttttttcgcg gcgatgccgt
     4501 cggcctgcct ttccttcagt aaggctcgtt caaactggct gaatgccgac atcatgtgca
     4561 gctgcagttc ctgcatcgga ttgtttgtgc cggcgctgaa cgtcagctgc tctttcagaa
     4621 aaataagggt aacgccctgg tcacgaagcc gggtggtgac agcgcacata tccgacatat
     4681 tccggcaaag gcgatcgatg gaatgaacca gaagcgtatc accagggcgt aattgcccca
     4741 ggagttcctg taaccccggg cggtttgtgt ccttcgcgct ggcatgctca acacaaattt
     4801 tatcaggctg aaaacccgcc tttgacaatg cctcttcctg acgggccgta ttctgatcgg
     4861 ccgagcttac cctgatgtag gcatattgca cgcaaaacct ccggaaggtc tttaaacatc
     4921 atagagattt aaagaataat ccataaacac tgatttaggg aggtttatag acattttttc
     4981 ggggatctga gaggcatgct gaaaacgtat acctttatag acatcagtca gcgacgacgg
     5041 cccagccaga taacttcagg ctccagtacc ggagatgcaa ggtcgagcct gctcagttgc
     5101 cggttgattt tgtggcggtt gaacctgaca gcatccaggt cgtcagcaaa agggcggatc
     5161 tcgccgaccg ttgtttcatc atccgcatta acgatagctt caagaaacgc cagggtttta
     5221 tcgaactcat ccgcagctgt ggctccgggc ataccatggc cgcttataca aaacggcgct
     5281 ttcagcgtag agttttcata aatggcctca acacgaatgt catgaagcca gtgctcaaag
     5341 aaattgtatt cataggtaaa gcgatcgcca gcatcaaaag caaaatcatc aatcacgacc
     5401 cggaacggat tatcgacaaa accgatgcct ccttcgtagg agataccgta atctttgcca
     5461 taaatgtgaa attgatggag atggtcgtcc ccccagccct gcactatctg gaaaatgaag
     5521 tgaagcgcgg ccagtgacgt gtcggcagca attctcagcc gacgccagac catcggactg
     5581 actccatgaa ctgctatttt gatgacgtag atcttcatag tcagcaggta ttgttatgaa
     5641 atgatggtgg ttattttaac ccctgtagat ggcatcattc gatggctgcc ccgaggtttt
     5701 tcatgctctc catcttgtaa caatcaggca taccacggag cttgtttgct t
//
LOCUS       DAECNO010000307         4954 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.guided.189, whole genome shotgun sequence.
ACCESSION   DAECNO010000307 DAECNO010000000
VERSION     DAECNO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.guided.189"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(36..3041)
                     /locus_tag="KKO08_004289"
     CDS             complement(36..3041)
                     /locus_tag="KKO08_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HBD6320266.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            3205..3762
                     /locus_tag="KKO08_004290"
     CDS             3205..3762
                     /locus_tag="KKO08_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBD6320267.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            3945..4805
                     /locus_tag="KKO08_004291"
     CDS             3945..4805
                     /locus_tag="KKO08_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HBD6320268.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         1244 a         1222 c         1233 g         1255 t
ORIGIN      
        1 taggggtctg acgctcagtg gaacgaaaac tcacgttaag caacgttttc tgcctctgac
       61 gcctctttta atggtctcag atgtcctttg gtcaccagtt ctgccagcgt gaaggaataa
      121 tggccgagca tattgatatg tccgtggcaa agcggggaga ggcgtgcgat atcttcatca
      181 ttcagtgttt caccctgcgc ccggagatga tccagggctg cctgcatata aatagtgttc
      241 cataacacga cggcgttagt gaccagcccc agtgtgccca gttgatcttc ctgaccgtcg
      301 gtatatcgtt ttcttatctc acctttttga ccgtgacaga tggctctggc aacggcatgg
      361 cgactttctc cccgattaag ctgggtcaga atgcgccggc ggtaatcttc atcatcaata
      421 taattaagca gatacagcgt tttgttgatg cgccccactt caatgattgc ctgagtcagt
      481 ccggaaggac gttcactttt cagcaatgaa cggaccagca ctgaaacctg tactttgccc
      541 agcttcaggg agccagcggt ccggatcatt tcgtcccact gaaggactat ttttcgggga
      601 tctgattgcc ctctggcaat atcattcagc acgccatagt cggcatcatg gtccattcgc
      661 cagaaaaccg aagcaccggc atcagccagg cgtggagaaa actggtatcc cagcagccag
      721 aaaaggccaa agacaagttc gctggcacct gctgtatcgg tcataatttc ggttggattc
      781 agcccggtct cctgttccag aagaccttcc agcacaaaga tagagtccct cagcgtcccc
      841 ggtataacga tgccatgaaa gccggaatac tgatcggaca caaagttgta ccaggtgatc
      901 cctctgttat taccaaagta tttgcggttc ggtccggcat tgattgttct gactggcgta
      961 acaaagcgca ttccatctgc agatgccact tctcctccac cccatatctg tgccagtggc
     1021 agcgttgcct gaaaatcaac cagtctggca ttagcgctgg tgatagtttc agcccgcaga
     1081 tagttcgctt ttgtccagtt cagccggtgt cgggtcagtg caggaacatt tgatctgatc
     1141 agtggttcca gaccgatatt gcaggcttca gccatcagca cggcgctgat gctgacgggc
     1201 agatcatcaa ctctggcact ggcttcacta gcatggaaaa actcatcagc aaatccggta
     1261 tgggcgttaa tttcgagcag caactccgtt aaatccaccg gagggagtag atcactgatc
     1321 attttgctca gtcgtttcag actgtccggc tcatcaagac tggcgagggg agaaattgtc
     1381 aaccggggct tcgggccaga aacatcgagt tcgacagcct cattttcgca aagacgtgca
     1441 gcaacctgtc tgtaacgact atcaagctga tgacccagag attttattgc ttcctgcggg
     1501 tctgtcgggt gccccaaaga acgataaacc ttaatccggt ttgcctgcca gtcagcaccc
     1561 tgtagtaatc ttgcacgagg atctccccac cggttactgc cggtaacgta gacatccctc
     1621 cgcctcagac tatcctgcag tttactgaga aagcagagcg tgtatcccct gcgggtgata
     1681 tgtttttcct tgttaatcac cagccgtttc catgaccgac tgataatttc cgttggtgcg
     1741 tcgtcaaaaa actgccgccg tgagctgaac tcccggctga ggtagtcaca ggcattcaga
     1801 gtggtaaccc cggcaggtgc ggatgaaaat ttaacggtat tcagcagatg gggcaggaaa
     1861 cgacgaacgc gcccgtactg ctccaccatt tcttcatgaa aattatcgtc tgagggccgg
     1921 gcaatttcac ggacaagcgt gatgatttca gccagctttt gccttgggat gtagctgaac
     1981 acctcagcac gaatcgattc gtccggtgtt tcttctttca gcaggtacga acatgcgctg
     2041 gcgagcgcca atgcagattt atccagatcc ttcagcgagc ggagccgttt tttctgccca
     2101 atctttctgg cgtcacggat gataacggcc agcatggcgt ccagaacgtc caatgcatca
     2161 tccagcgcca gcgtttccca tgcaaggaca aaggcaacca gaaccgccat ccttttctgc
     2221 ggtgacatcc tggcaatatt gaacaccgaa gtcataccag cataacgtgc gagatttttc
     2281 aggcgcacag ccgggagtgt actcaggttt tcagcatgca ggccaaaatc gttcagagtt
     2341 ttccagcgtt caattgcttc attaaacgcc ggaccactga tggtcacagg gccctttttc
     2401 agtgattcca gtaaagacag gcggctgcaa tcagttggcc ccagcagcat ctccagctgt
     2461 gaacgctgtt cggctgacgg tatcagtgcc agtttgttcc acaggcgcaa cgtcgccttt
     2521 tcccttacct ctgaaatcaa ccgggtcagc gtagtggctc cggggagaat aatacgatgt
     2581 tgcataagcc accctgtcgc cagatcgaaa agcaggccag gacgttcgtt gcttatccag
     2641 ctccgggtat ataaaagacg ggtaaggcga aatgtccagg gccaggcaaa ttcacgatac
     2701 tgatagtgct gacgtatcag cgctgcatgc tcacggcggg tattttccct ctgaccgtat
     2761 tctgcaagaa cggtgatatc acgaatcccg agctgtctgg cggtaaaatg ccggacgccg
     2821 gaaggaatat gattcatatc ggtgaggaag gtgcccagaa aacggacaca tccaatttgc
     2881 agggcaatgc ccagacggtt gtgatcacct ctgctttttc cgataaattc cttgtctgct
     2941 tcatcaaggt gaaaatatcg tgccagctga agctcatccg gttcaccggt gaatctgcca
     3001 tagctttcag tctgctcagt ggtcagaaag tcaacgggca tatcggcctc cctgcctgac
     3061 gggcatttag taacattttt ccaaccgtac gaaatgttat aaattatcgg acatcgtaaa
     3121 actgttacat taatatgtct attaaatcgt aaatttgtaa taatagacat gagttgtccg
     3181 atattcgatt taaggtacat ttttatgcga ctttttggtt acgctcgggt ctcaaccagt
     3241 cagcagtctc ttgatcttca ggtcagagca ctcaaagacg caggtgtgaa agcaaaccgt
     3301 atatttaccg ataaggcatc cggcagttca acagaccggg aagggctgga tttgctgagg
     3361 atgaaggtgg aggaaggtga tgtcattctg gttaagaagc tcgaccgtct tggccgcgac
     3421 actgccgata tgatccaact gataaaggaa tttgacgctc agggcgtggc agtccggttc
     3481 attgatgacg ggatcagtac cgacggtgat atggggcaaa tggtggtcac catcctgtcg
     3541 gctgtggcac aggctgaacg ccggaggatc ctagaacgca cgaatgaggg ccgacaggaa
     3601 gcaaagctga aaggaatcaa atttggccgc aggcgtaccg tggacaggaa cgtcgtgctg
     3661 acgcttcatc agaagggcac tggtgcaacg gaaattgctc atcagctcag tattgcccgc
     3721 tccacggttt ataaaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     3781 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3841 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3901 aataaccctg gtaaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3961 ttcgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4141 tgatgagcac ttttaaagtt ctgctatgtg gtgcggtatt atcccgtgtt gacgccgggc
     4201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4321 ccatgagtga taacactgct gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     4501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4801 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4921 gtgagttttc gttccactga gcgtcagacc ccta
//
LOCUS       DAECNO010000308         2767 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.guided.216, whole genome shotgun sequence.
ACCESSION   DAECNO010000308 DAECNO010000000
VERSION     DAECNO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.guided.216"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            177..1181
                     /locus_tag="KKO08_004292"
     CDS             177..1181
                     /locus_tag="KKO08_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HBD6320269.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(1363..1539)
                     /locus_tag="KKO08_004293"
     CDS             complement(1363..1539)
                     /locus_tag="KKO08_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBD6320270.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            1869..2684
                     /gene="sul2"
                     /locus_tag="KKO08_004294"
     CDS             1869..2684
                     /gene="sul2"
                     /locus_tag="KKO08_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBD6320271.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          649 a          722 c          711 g          685 t
ORIGIN      
        1 gggagcacat caacctcacc ggcgattacc tctggcgcag cagcgccaag atcggcgcgg
       61 gcaagttcag gccgctacga ccgctgcaac cggcttagcg tgctttattt aatgagatgg
      121 tcactccctc cttcccagta ctatgctgag gacaggcttt cattcggaga accatcatgg
      181 aaaacattgc gcttattggt atcgatctgg gtaagaactc tttccatatt cattgtcagg
      241 atcatcgtgg gaaggccgtt taccgtaaaa aattcacccg accaaagcta atcgaatttc
      301 tggcgacatg cccggcaaca accatcgcga tggaagcctg tggcggttct cactttatgg
      361 cacgcaagct ggaagagtta gggcattttc caaagctgat atcaccgcaa tttgtccgcc
      421 cattcgttaa aagcaacaaa aatgacttcg ttgatgctga agctatctgt gaagcagcat
      481 cacgtccatc tatgcgtttc gtgcagccca gaaccgaatc tcagcaggca atgcgagctc
      541 tgcatcgtgt ccgtgaatcc ctggttcagg ataaggtgaa aacaactaat cagatgcatg
      601 cttttctgct ggaatttggt atcagcgttc cgcgaggtgc tgccgttatt agtcgactga
      661 gtacccttct tgaggacagt agtttgcctc tttatctcag ccagttactg ctgaaattac
      721 aacagcatta tcactatctt gttgagcaga ttaaagatct ggaatctcag ttgaaacgaa
      781 agttggacga agatgaggtt ggacagcgct tgctgagtat tccctgcgtt ggaacgctga
      841 ctgccagtac tatttcaact gagattggcg acgggaagca gtacgccagc agccgtgact
      901 ttgcggcggc aacagggctg gtaccccgac agtacagcac gggaggtcgg acgacattgt
      961 tagggattag caagcggggc aacaaaaaga tccgaacttt gttggttcag tgtgccaggg
     1021 tattcataca aaaactggaa caccagtctg gcaagttggc cgactgggtc agggagttgt
     1081 tgtgtcggaa aagcaacttt gtcgtcacct gtgctctggc aaacaagctg gccagaatag
     1141 cctgggcact gacggcgcga cagcaaactt acgaagcata aaggcagaaa tacaccagtt
     1201 taaacaatca ttcatctggt tttgcgaata ctgatattga tgatactaac ggcccaccgg
     1261 cctgttgagg aacctgtaaa acggaaaggc tcattgaagc cgtatatttt ctggaggttc
     1321 atcaggcgcg gaactcatcg aggcgcggga ataaaatccc attcagacgc cggatagatt
     1381 caagcaagcc aacttgtcgt caaaatcggt gttgcaaaaa cgggagtgac catagattcc
     1441 gttttctgag gcgaccccca catagagacc ttcccggtca ttcactttgt agagtttgtc
     1501 cctgggcttg agattgcgca gcttggtatc ggtcagcatg taccttgtcc ttcttcgtct
     1561 ccgataccca ctctattgta aacaagacat ttttatcttt tatattcaat ggcttatttt
     1621 cctgctaatt ggtaatacca tgaaaaatac catgctcaga aaaggcttaa caatattttg
     1681 aaaaattgcc tactgagcgc tgccgcacag ctccataggc cgctttcctg gctttgcttc
     1741 cagatgtatg ctattctgct cctgcagcta atggagcacc gcaaacaggt tactcgcctg
     1801 gggattccct ttcgacccga gcatccgtat gagactcatg ctcgattatt attattatag
     1861 aagcccccat gaataaatcg ctcatcattt tcggcatcgt caacataacc tcggacagtt
     1921 tctccgatgg aggccggtat ctggcgccag acgcagccat tgcgcaggcg cgtaagctga
     1981 tggccgaggg ggcagatgtg atcgacctcg gtccggcatc cagcaatccc gacgccgcgc
     2041 ctgtttcgtc cgacacagaa atcgcgcgta tcgcgccggt gctggacgcg ctcaaggcag
     2101 atggcattcc cgtctcgctc gacagttatc aacccgcgac gcaagcctat gccttgtcgc
     2161 gtggtgtggc ctatctcaat gatattcgcg gttttccaga cgctgcgttc tatccgcaat
     2221 tggcgaaatc atctgccaaa ctcgtcgtta tgcattcggt gcaagacggg caggcagatc
     2281 ggcgcgaggc acccgctggc gacatcatgg atcacattgc ggcgttcttt gacgcgcgca
     2341 tcgcggcgct gacgggtgcc ggtatcaaac gcaaccgcct tgtccttgat cccggcatgg
     2401 ggttttttct gggggctgct cccgaaacct cgctctcggt gctggcgcgg ttcgatgaat
     2461 tgcggctgcg cttcgatttg ccggtgcttc tgtctgtttc gcgcaaatcc tttctgcgcg
     2521 cgctcacagg ccgtggtccg ggggatgtcg gggccgcgac actcgctgca gagcttgccg
     2581 ccgccgcagg tggagctgac ttcatccgca cacacgagcc gcgccccttg cgcgacgggc
     2641 tggcggtatt ggcggcgctg aaagaaaccg caagaattcg ttaactgcac attcgggata
     2701 tttctctata ttcgcgcttc atcagaaaac tgaaggaacc tccattgaat cgaactaata
     2761 ttttttt
//
LOCUS       DAECNO010000309         2711 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.guided.218, whole genome shotgun sequence.
ACCESSION   DAECNO010000309 DAECNO010000000
VERSION     DAECNO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.guided.218"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..471
                     /locus_tag="KKO08_004295"
     CDS             <1..471
                     /locus_tag="KKO08_004295"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBD6320272.1"
                     /translation="PQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIA
                     ALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLR
                     ALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            532..1335
                     /locus_tag="KKO08_004296"
                     /note="aph(3'')-Ib"
     CDS             532..1335
                     /locus_tag="KKO08_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBD6320273.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1335..2171
                     /locus_tag="KKO08_004297"
     CDS             1335..2171
                     /locus_tag="KKO08_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBD6320274.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2278..2682)
                     /locus_tag="KKO08_004298"
                     /pseudo
     CDS             complement(2278..2682)
                     /locus_tag="KKO08_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          548 a          769 c          781 g          613 t
ORIGIN      
        1 ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag
       61 gcagatcggc gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac
      121 gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc
      181 ggcatggggt tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc
      241 gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt
      301 ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag
      361 cttgccgccg ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc
      421 gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt
      481 cgggatattt ctctatattc gcgcttcatc agaaaactga aggaacctcc attgaatcga
      541 actaatattt tttttggtga atcgcattct gactggttgc ctgtcagagg cggagaatct
      601 ggtgattttg tttttcgacg tggtgacggg catgccttcg cgaaaatcgc acctgcttcc
      661 cgccgcggtg agctcgctgg agagcgtgac cgcctcattt ggctcaaagg tcgaggtgtg
      721 gcttgccccg aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt ggtgataacg
      781 gcaattccgg gagtaccggc ggctgatctg tctggagcgg atttgctcaa agcgtggccg
      841 tcaatggggc agcaacttgg cgctgttcac agcctatcgg ttgatcaatg tccgtttgag
      901 cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa tgccgtcaat
      961 cccgacttct taccggacga ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc
     1021 gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg atatggttgt ttgccatggt
     1081 gatccctgca tgccgaactt catggtggac cctaaaactc ttcaatgcac gggtctgatc
     1141 gaccttgggc ggctcggaac agcagatcgc tatgccgatt tggcactcat gattgctaac
     1201 gccgaagaga actgggcagc gccagatgaa gcagagcgcg ccttcgctgt cctattcaat
     1261 gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct tctatctgcg attggaccct
     1321 ctgacttggg gttgatgttc atgccgcctg tttttcctgc tcattggcac gtttcgcaac
     1381 ctgttctcat tgcggacacc ttttccagcc tcgtttggaa agtttcattg ccagacggga
     1441 ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat tgctgatgaa ctgcgcgggg
     1501 ccgactatct ggtatggcgc aatgggaggg gagcagtccg gttgctcggt cgtgagaaca
     1561 atctgatgtt gctcgaatat gccggggagc gaatgctctc tcacatcgtt gccgagcacg
     1621 gcgactacca ggcgaccgaa attgcagcgg aactaatggc gaagctgtat gccgcatctg
     1681 aggaacccct gccttctgcc cttctcccga tccgggatcg ctttgcagct ttgtttcagc
     1741 gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg gcgattatag
     1801 ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct acatggcgat ctgcatcatg
     1861 aaaacatcat gttctccagt cgcggctggc tggtgataga tcccgtcggt ctggtcggtg
     1921 aagtgggctt tggcgccgcc aatatgttct acgatccggc tgacagagac gacctttgtc
     1981 tcgatcctag acgcattgca cagatggcgg acgcattctc tcgtgcgctg gacgtcgatc
     2041 cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct ttccgcagct tggaacgcgg
     2101 atggagaaga ggagcaacgc gatctagcta tcgcggccgc gatcaagcag gtgcgacaga
     2161 cgtcatacta gatatcaagc gacttctcct atcccctggg aacacatcaa tctcaccgga
     2221 gaatatcgct ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgaccccaaa
     2281 caggaaacgc agctgaaacg ggaagctcaa cacccactga cgcatgggtt gttcaggcag
     2341 tacttcatca accagcaagg cggcactttc ggccatccgc cgcgccccac agctcgggca
     2401 gaaaccgcga cgcttacagc tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg
     2461 aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa attcccgttg
     2521 cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata
     2581 ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt
     2641 atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat
     2701 actgtgttta c
//
LOCUS       DAECNO010000310         2710 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.guided.219, whole genome shotgun sequence.
ACCESSION   DAECNO010000310 DAECNO010000000
VERSION     DAECNO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.guided.219"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            <1..471
                     /locus_tag="KKO08_004299"
     CDS             <1..471
                     /locus_tag="KKO08_004299"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBD6320275.1"
                     /translation="PQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIA
                     ALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLR
                     ALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            576..1334
                     /locus_tag="KKO08_004300"
     CDS             576..1334
                     /locus_tag="KKO08_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBD6320276.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1334..2170
                     /locus_tag="KKO08_004301"
     CDS             1334..2170
                     /locus_tag="KKO08_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBD6320277.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2277..2681)
                     /locus_tag="KKO08_004302"
                     /pseudo
     CDS             complement(2277..2681)
                     /locus_tag="KKO08_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          548 a          769 c          781 g          612 t
ORIGIN      
        1 ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag
       61 gcagatcggc gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac
      121 gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc
      181 ggcatggggt tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc
      241 gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt
      301 ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag
      361 cttgccgccg ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc
      421 gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt
      481 cgggatattt ctctatattc gcgcttcatc agaaaactga aggaacctcc attgaatcga
      541 actaatattt ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg
      601 gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc
      661 gccgcggtga gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg
      721 cttgccccga ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg
      781 caattccggg agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt
      841 caatggggca gcaacttggc gctgttcaca gcctatcggt tgatcaatgt ccgtttgagc
      901 gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc
      961 ccgacttctt accggacgag gacaagagta cgccgcagct cgatcttttg gctcgtgtcg
     1021 aacgagagct accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg
     1081 atccctgcat gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg
     1141 accttgggcg gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg
     1201 ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg
     1261 tattggggat cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc
     1321 tgacttgggg ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc
     1381 tgttctcatt gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac
     1441 tcctgcaatc gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc
     1501 cgactatctg gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa
     1561 tctgatgttg ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg
     1621 cgactaccag gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga
     1681 ggaacccctg ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg
     1741 ggcgcgcgat gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc
     1801 cgatcaaatg atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga
     1861 aaacatcatg ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga
     1921 agtgggcttt ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct
     1981 cgatcctaga cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc
     2041 gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga
     2101 tggagaagag gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac
     2161 gtcatactag atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag
     2221 aatatcgctg gccaaagcct tagcgtagga ttccgcccct tcccgcaaac gaccccaaac
     2281 aggaaacgca gctgaaacgg gaagctcaac acccactgac gcatgggttg ttcaggcagt
     2341 acttcatcaa ccagcaaggc ggcactttcg gccatccgcc gcgccccaca gctcgggcag
     2401 aaaccgcgac gcttacagct gaaagcgacc aggtgctcgg cgtggcaaga ctcgcagcga
     2461 acccgtagaa agccatgctc cagccgcccg cattggagaa attcttcaaa ttcccgttgc
     2521 acatagcccg gcaattcctt tccctgctct gccataagcg cagcgaatgc cgggtaatac
     2581 tcgtcaacga tctgatagag aagggtttgc tcgggtcggt ggctctggta acgaccagta
     2641 tcccgatccc ggctggccgt cctggccgcc acatgaggca tgttccgcgt ccttgcaata
     2701 ctgtgtttac
//
LOCUS       DAECNO010000311         1732 bp    DNA     linear   BCT 24-MAY-2021
DEFINITION  TPA_asm: Shigella flexneri strain SFL1416 isolate 59
            SAMN14086404-rid12263853.guided.245, whole genome shotgun sequence.
ACCESSION   DAECNO010000311 DAECNO010000000
VERSION     DAECNO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN14086404
            Sequence Read Archive: SRR11285421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001043781.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001043781.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2021 09:04:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,206
            Genes (coding)                    :: 3,857
            CDSs (with protein)               :: 3,857
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 349
            CDSs (without protein)            :: 349
            Pseudo Genes (ambiguous residues) :: 0 of 349
            Pseudo Genes (frameshifted)       :: 157 of 349
            Pseudo Genes (incomplete)         :: 84 of 349
            Pseudo Genes (internal stop)      :: 140 of 349
            Pseudo Genes (multiple problems)  :: 30 of 349
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN14086404-rid12263853.guided.245"
                     /strain="SFL1416"
                     /isolate="59"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
     gene            complement(43..882)
                     /gene="intI1"
                     /locus_tag="KKO08_004303"
                     /pseudo
     CDS             complement(43..882)
                     /gene="intI1"
                     /locus_tag="KKO08_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
     gene            1038..1511
                     /locus_tag="KKO08_004304"
     CDS             1038..1511
                     /locus_tag="KKO08_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HBD6320278.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          428 a          454 c          472 g          378 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc tggcggaggg tgtgcggtgt
       61 ggcgggcttc gtgatgcctg cttgttctac ggcacgtttg aaggcgcgct gaaaggtctg
      121 gtcatacatg tgatggcgac gcacgacacc gctccgtgga tcggtcgaat gcgtgtgctg
      181 cgcaaaaacc cagaaccacg gccaggaatg cccggcgcgc ggatacttcc gctcaagggc
      241 gtcgggaagc gcaacgccgc tgcggccctc ggcctggtcc ttcagccacc atgcccgtgc
      301 acgcgacagc tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc
      361 cttggagccc ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac
      421 ccgcagttgc aaaccctcac tgatccgcat gcccgttcca tacagaagct gggcgaacaa
      481 acgatgctcg ccttccagaa aaccgaggat gcgaaccact tcatccgggg tcagcaccac
      541 cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga agccagggca gatccgtgca
      601 cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc tgacgatgcg tggagaccga
      661 aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc tgcccaaggt
      721 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
      781 ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
      841 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
      901 ttttgtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
      961 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     1021 aacccaggat gagaaccttg aaagtatcat tgatggctgc gaaagcgaaa aacggcgtga
     1081 ttggttgcgg tccagacata ccctggtccg cgaaagggga gcagctactt tttaaagcat
     1141 tgacctacaa tcagtggctt ctggtgggtc gcaagacgtt tgaatctatg ggcgcactcc
     1201 ccaataggaa atacgcggtc gttacccgct caggttggac atcaaatgat gacaatgtag
     1261 ttgtatttca gtcaatcgaa gaggccatgg acaggctagc tgaattcacc ggtcacgtta
     1321 tagtgtctgg tggcggagaa atttaccgag aaacattacc catggcctct acgctccact
     1381 tatcgacgat cgacatcgag ccagaggggg atgttttctt cccgagtatt ccaaatacct
     1441 tcgaagttgt ttttgagcaa cactttactt caaacattaa ctattgctat caaatttgga
     1501 aaaagggtta acaaagctat gcaatcgacg gcaaaaagct tcgttcgctt cgcgcactac
     1561 gccttttccg cgattgatag cgacgttatg tgaatattga aatgagcatt ccaaagaaag
     1621 gaagtagaaa aattatcgtg ggcgaaaatg agtttttgtg gctcattagg tcaaaactca
     1681 catattcgca ggacggcact gttgcaaata gtcggtggtg ataaacttat ca
//
